LOCUS       DASSRL010000001         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1242311, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000001 DASSRL010000000
VERSION     DASSRL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242311"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1150
                     /locus_tag="VFL49_00005"
     CDS             11..1150
                     /locus_tag="VFL49_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7910392.1"
                     /translation="MDFELSEEQRAFQATARIFARSEMMPNARAWDEGEVFPVDALRK
                     AAALGFGGIYVKEDVGGSALSRLDAALIFEELAQGCTSTAAYISIHNMAAWMIDAFGS
                     AEARKKFLPKLCAMDHFASYCLTEPGAGSDAANLSTRARRDGEHYVLDGSKAFISGGG
                     VSDIYVVMARTGESGPRGISCIVLEKGTPGLSFGAPEKKLGWKTQPTAMVIFENCRVP
                     VSNLIGTEGQGFKIAMAGLDGGRLNIGACSIGGAQFCLDRTVDYMKERRQFGSRLADF
                     QALQFRIADYATEIDAARLLLHRAAVAVGAGEPAATRLAAQAKRLATDTGFEVVNGCL
                     QLHGGYGYLRDHPIERVLRDVRVHQILEGTNEIMRLIVSRAMLGN"
     gene            1169..1732
                     /locus_tag="VFL49_00010"
     CDS             1169..1732
                     /locus_tag="VFL49_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910393.1"
                     /translation="MEQLPRLYLTRAEMEKRIARFKDLKGFDGGLPDSKMPGCERTLF
                     NVIGFQPPAGGGGAVTSPVGDDAARLAAIKISEGFNLGYCKAKPGHGPEMHNHDTNET
                     FIPMTGTWRASWEDKEGNVHFVDLGPLDVVSFPPGMIRRFENVTKGDPTAESVLMFVI
                     GGDAPRAEFTDQAMQRLENAGVWPPHA"
     gene            1747..>2456
                     /locus_tag="VFL49_00015"
     CDS             1747..>2456
                     /locus_tag="VFL49_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyryl-CoA hydrolase"
                     /protein_id="HET7910394.1"
                     /translation="MGSLDDDILFERRGAAGLVTLNRPKALNAVTHDMVRALRVQLDR
                     WADDAAVARVVIMAAGERAFSAGGDIRALYDLGKAGRVEDALQFWRDEYPLNMAIKNY
                     RKPYVALIEGIAMGGGVGVSVHGSHRVAGDRFSFAMPEVGIGFFPDVGATWFLPRMPG
                     ELGAYCALTGERFGPADAISSGVATHRIASKHFPALLAGLTGMVSVDAMLAAFALPAE
                     RGPIALRQAAIDRLFAANR"
BASE COUNT          436 a          722 c          797 g          501 t
ORIGIN      
        1 cggggctttc atggattttg agctttccga agaacaacgg gcgtttcagg caaccgcacg
       61 catctttgcg cgtagcgaga tgatgccgaa cgcgcgtgcc tgggacgaag gcgaggtctt
      121 tcctgtcgat gccttgcgca aggcggccgc tctcggtttt ggcggcatct acgtcaaaga
      181 ggacgtcggc ggatcggccc tgtcgcgtct cgacgccgca ttgatcttcg aggagttggc
      241 gcaaggatgc acgtcgaccg ccgcctatat ctcgatccac aacatggcgg cctggatgat
      301 cgatgccttc ggcagcgcgg aggcgcgcaa aaagtttttg ccgaagctct gcgccatgga
      361 tcatttcgcg agctattgcc tgactgagcc aggcgcgggc tccgacgcgg ccaatttgtc
      421 cacgcgcgcg cgccgcgacg gcgagcacta tgttctcgac ggcagcaagg cttttatttc
      481 gggtggtggc gtctcggaca tctatgttgt gatggcacgc acgggcgaga gcggtccgcg
      541 cggaatatcc tgcattgttc tcgagaaggg gacgcctggc ctgtcgttcg gcgcgccgga
      601 aaagaagctc ggctggaaaa cgcagccgac cgccatggtg atattcgaaa attgccgcgt
      661 cccggtttca aacctcatcg gtaccgaggg gcagggcttc aagatcgcaa tggccgggct
      721 cgatggcggc cggctgaata tcggggcctg ctcgatcggg ggtgcgcagt tctgtctcga
      781 ccgcactgtc gactacatga aggagcgtcg acagttcggc tccaggctcg ccgattttca
      841 ggcgttgcaa ttccgcatcg ccgactatgc gaccgagatt gatgccgcgc ggctgctgct
      901 gcaccgtgcc gcagtcgccg tcggcgcagg cgagcccgcc gcgacaaggc ttgccgcaca
      961 ggccaagcgc cttgccactg acacagggtt tgaggtggtc aacggctgcc tgcaactgca
     1021 tggcggttat ggttatctgc gcgatcaccc gattgagcgc gtgttgcgcg atgtgcgcgt
     1081 gcatcaaatc cttgaaggca ccaacgagat catgcgcctg atcgtcagcc gcgctatgct
     1141 ggggaattag aaggtcaggg aggataggat ggaacagctt ccgcgtctct atcttacgcg
     1201 cgccgagatg gaaaagcgca tcgctcgttt caaggacttg aagggattcg acggaggtct
     1261 gccggacagc aagatgccgg gctgcgagcg cacgctattc aatgtgatcg gctttcagcc
     1321 gccggcgggc ggtggaggtg cggtgacttc gccagtcggc gacgacgcgg ccaggctcgc
     1381 cgccatcaag atcagcgagg ggttcaatct cggttactgc aaggcgaagc ccggccacgg
     1441 gccggaaatg cacaatcacg atacaaatga gactttcatc cccatgacgg gcacttggcg
     1501 tgcgagctgg gaggataagg aaggcaacgt tcacttcgtc gatcttgggc ccctcgatgt
     1561 tgtctctttc ccacccggca tgatccgccg ctttgagaac gtcacaaaag gcgacccgac
     1621 tgccgaatcg gttcttatgt tcgtgattgg tggtgatgcg ccacgcgcgg aatttaccga
     1681 tcaggccatg cagcgcctgg aaaatgcggg cgtgtggccc ccgcacgcct aatcacgtca
     1741 agcgacatgg gttctctcga cgacgacatt ctgttcgagc gtcgtggtgc ggccggcctc
     1801 gttacgctca accggcccaa ggcgctcaat gccgtcaccc atgacatggt gcgtgcgctg
     1861 cgcgtgcagc tcgatcgttg ggccgatgat gcggcggtcg cgcgggtcgt catcatggcg
     1921 gcaggggagc gcgccttctc ggcgggcggc gacatccgcg cgctctacga tctcggcaaa
     1981 gccggccgcg tcgaggacgc gctgcaattc tggcgtgatg aatacccgct caatatggcg
     2041 atcaaaaact atcgcaagcc gtatgtcgcg ttgatcgagg gcatcgcgat gggcggcgga
     2101 gtcggcgtat cggtgcacgg ttcgcatcgc gttgccggtg accgattttc gttcgcgatg
     2161 ccggaagtcg gcatcggctt ctttccggat gtcggcgcaa cctggtttct gccgcgcatg
     2221 ccgggcgaac ttggtgcgta ttgtgcgtta accggcgaac gcttcggacc tgccgacgcg
     2281 atatcgtccg gtgttgccac tcaccgcatt gcctcaaagc actttcctgc gcttctcgct
     2341 gggctgaccg gaatggtttc cgtcgatgcc atgctcgccg cctttgccct gccggcggag
     2401 agggggccga tcgcgctgcg ccaggccgca atcgatcgtc tttttgcggc aaatcg
//
LOCUS       DASSRL010000002         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1207385, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000002 DASSRL010000000
VERSION     DASSRL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207385"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFL49_00020"
     CDS             complement(<1..760)
                     /locus_tag="VFL49_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7910395.1"
                     /translation="MTAAVVLLATNSVSMAEKGEVDPGQVSEGLKAIFSYGQGRTTRD
                     RLNANTTTIITGTIGGTYVQIGADLASALDNGDDLRILPIVGRGSVQSIADILYLKGV
                     DVGVIRSDTLDYLEKKGYATNLKNQLSYITKLYNEEMHVVAPKSIAKLSDLAGKNVAV
                     DLPNGGTFITAITVFERLGIKANFVYIEQRVAYEKLRKGEIDAMIAVQGVPSKFTTTI
                     KDERFHLVPVDYAPALQGDYLPSQLKAEDYPALIP"
     gene            complement(823..1935)
                     /locus_tag="VFL49_00025"
     CDS             complement(823..1935)
                     /locus_tag="VFL49_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910396.1"
                     /translation="MSSPIHDGSISPNDPSYYAPRRSGVEAKTPLPDRLSSPSALATT
                     DSGSARTEDAEVIVPFPRAREYLNVFDHGTRKTWRTKALVVSAIAAALTGGIAFFGFV
                     SFAPRVSEKQTQQPELSLATRLQTAAVDIQKAAQRVVTPTLVVNESAGEMNAPLALGL
                     EVKNYSPGAVIALSDFPAGTSISSGAAEGDRHWRIAVEELPNAKVTPPSGYIGVMTIL
                     AELRIGKDIPIVRSPVRLIWRALPSPASPAPGPSSAPEPSPPRTTAVDQPVRHLDARE
                     SASLLRRAEEMKAAGDLAAARLLLQRVAETNNARAAFELAETYDPIAIKKAGGSSVTA
                     DAALAQHWYQRAREWGHPSGSQELDAIASRAAASPK"
     gene            complement(2038..2619)
                     /locus_tag="VFL49_00030"
     CDS             complement(2038..2619)
                     /locus_tag="VFL49_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7910397.1"
                     /translation="MSKATPSTQVILRHWREAVPDDRLAHLVKDATRALVRALQMRLG
                     EHGVSFGHWTFLRILWESDGLTQRQLSEQAGVMEPTTFSAVKAMERLGYVVRRRHPGN
                     RKKMYVFLTPKGRGLRKRLVPLAEDVNRIAVRHVRSADIARARKLLLSIIENLARDEH
                     RADMRMPSTRELGRLNGSVRSTRPKGKRKGRVS"
     gene            complement(2616..3776)
                     /locus_tag="VFL49_00035"
     CDS             complement(2616..3776)
                     /locus_tag="VFL49_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7910398.1"
                     /translation="MADPTTPPLRLGVAGLGRAFSVMLPTFIRHPAVTLIAAADPRPE
                     ARKRFADDFSAKAYDTIAELCADPAVDAVYIATPHQFHSEHATLAARARKHLLIEKPM
                     ALTLKDCKAIVEATRRSGVQVVVGHSHSFDPPVRHLRKLIESGAYGAVRMITALNYTD
                     YLYRPRRPEELDTSKGGGAIFNQAAHQVDIVRLVAGAAVTRVRAMTGAWDPKRPTEGA
                     YAALLTFANGGFASLIYNGYGHFDSDEFDGWVGEMGQSKTPYPAQARPSFKTQDDEIA
                     FKNDRNYGGAKFRPPIETDAHQHFGTLLVSCERGDLRPVPNGVMIYQNGTARIDKIPS
                     PRVARAEVIDELYDAVVHGKTPLHDAVWGTATVEVLLAMLQSAHDGADVNLT"
     gene            3869..>4431
                     /locus_tag="VFL49_00040"
     CDS             3869..>4431
                     /locus_tag="VFL49_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7910399.1"
                     /translation="MLTSEENDLLCRVEGNAPMGRIMRRHWIAACLSEEVAEPDGAPV
                     KVRLLGEDLVVFRDSKGRVGVLDEYCSHRRASLLFARNEDCGLRCLYHGWKFDVDGNI
                     VEMASEPDNSLIFDRVKHKSYPTREAGGFVWAYMGPPEEMREFEPPAFAPTPDARVSA
                     TKVRVNCNWAQILEGQIDSAHSSHLHSS"
BASE COUNT          777 a         1311 c         1436 g          907 t
ORIGIN      
        1 caggtatcag ggcgggatag tcctcggcct ttagctgtga gggcagataa tcgccttgca
       61 gtgcgggcgc atagtccacc ggcaccagat gaaatctttc gtccttgatc gtggtcgtga
      121 acttcgaggg aacaccctgc acggcgatca tcgcgtcaat ttcgcccttc cgcagctttt
      181 cataagccac gcgctgctcg atgtagacga agttggcctt gatgccgagc cgctcgaaaa
      241 ccgtgatcgc ggtaatgaat gtgccaccat tcggcaggtc gacggcgaca ttctttcccg
      301 ccaggtcaga cagcttcgcg atcgatttgg gcgcgacgac gtgcatctcc tcgttgtaga
      361 gctttgtaat gtaggaaagc tggttcttga ggttggtggc gtatcccttc ttttccaggt
      421 agtcgagcgt gtcggatcgg ataacgccga cgtcgacacc tttgagatac agaatatcgg
      481 caatgctctg gaccgagccg cgtccaacga tcggaaggat gcgaagatcg tcaccgttat
      541 cgagcgcgga agcgagatcg gcgccgatct ggacataggt gccgccgatt gtgccggtga
      601 tgatcgtggt cgtattggcg ttgaggcgat ctctggtcgt gcggccttgc ccgtagctga
      661 agattgcctt cagcccttcg ctcacctggc ccggatcaac ttcgcctttt tcggccatgc
      721 tgacggaatt tgtggcaagc agcacgacgg ctgccgtcat gaggcatgca aagcggacgg
      781 cactcaacat tgtagacatg gtcattggcc cccattctgg cgttatttcg gactagcggc
      841 cgcgcggctt gcaattgcgt cgagctcctg agatccgctc ggatgccccc attcgcgggc
      901 gcgctgatac cagtgctggg cgagagcagc gtcggcggtg acgctgctcc cgcctgcttt
      961 cttgatggcg atcgggtcat aggtctcggc aagctcaaat gccgcgcgcg cgttgttcgt
     1021 ctccgccacg cgctgcagca ggagccgggc agccgcgaga tcgccggcag ccttcatctc
     1081 ttccgcgcgc cgcagaagtg acgcgctctc cctcgcatcg agatgcctga ccggttggtc
     1141 gaccgccgtc gttctgggcg gcgaaggttc tggcgcggac gaggggccgg gcgcaggtga
     1201 ggcaggtgag gggagcgcgc gccagatcag gcgcacgggg ctgcgcacga tcggaatatc
     1261 tttaccgatc cgcagctcgg ccaagatcgt catgacaccg atgtaaccgc tcggcggagt
     1321 gaccttggcg ttcggcaatt cctccaccgc aatccgccag tgtcggtctc cttcggcggc
     1381 tccgctgctg atgctcgtgc cggcgggaaa atcgctcagc gcaatgacgg cgccggggct
     1441 gtagtttttg acctcgaggc caagcgcgag cggcgcgttc atttctcccg cggactcatt
     1501 taccaccaaa gtcggggtca ccacccgttg cgctgccttc tggatatcga cggcggcggt
     1561 ttgcaggcgg gtcgcaagcg agagttccgg ttgctgtgtt tgcttttcac tgactcgcgg
     1621 tgcgaagctc acgaatccga aaaacgcaat accgcccgta agggcggcgg cgattgccga
     1681 gacaacgagc gctttggtgc gccaggtctt gcgtgtcccg tggtcaaaca cgttcaggta
     1741 ttcgcgtgcg cgcgggaaag gaacaatgac ctcggcgtcc tcggttcgcg cgctcccgct
     1801 gtcggttgtc gctaaagctg agggggatga gagccggtcc gggagcggag tcttggcctc
     1861 aacgcctgac cggcgcggtg cgtaatagct gggatcgttc ggggaaattg acccatcgtg
     1921 gatcggagaa ctcatacgct gcccccacgg cacagaagtt ccggctggta ttgtgggagc
     1981 ggcaacgtgg caaatttctg ttcgtccaat tctccgaatg caaagtttat ggaggtatta
     2041 cgacacccga cccttccgct tgccctttgg ccgggtactc cgcaccgatc cgttgagccg
     2101 tccgagctcg cgggtcgacg gcatccgcat gtcggccctg tgctcgtcgc gcgccaggtt
     2161 ttctattatg gagagcaata gcttacgtgc gcgggcgatg tcggctgaac ggacgtggcg
     2221 gacggcgatc cggttcacgt cttcggcgag cgggacaagc cttttacgca agccgcggcc
     2281 cttcggggtc aggaagacat acatcttctt ccggttgccg ggatgccggc ggcgaacgac
     2341 gtaaccgaga cgctccatcg ccttgaccgc cgaaaaggtg gtcggttcca tgacgccggc
     2401 ctgctcgctt aattggcgct gggtcagtcc gtcggattcc caaaggatgc gcaggaacgt
     2461 ccagtgtcca aaagatacgc catgttcgcc tagtcgcatt tgcagggccc gcactagcgc
     2521 gcgcgttgca tcctttacga gatgcgcgag gcggtcatcc ggcaccgcct cgcgccagtg
     2581 ccggaggatc acctgcgtgc tcggcgtggc tttgctcatg tgaggttcac gtcggcgccg
     2641 tcgtgtgccg actgcagcat cgccagcagc acctcgaccg tcgccgttcc ccagacagca
     2701 tcatgcagag gtgttttgcc gtgcacgacg gcgtcgtaaa gctcgtcgat gacttcggcg
     2761 cgcgccacgc gaggcgaggg gattttgtcg atccgcgccg ttccgttctg gtagatcatg
     2821 actccattgg gcaccggacg caggtcgccc cgctcgcagc tcaccagcaa cgtgccgaaa
     2881 tgctgatgcg catccgtttc gatgggcgga cggaatttgg caccgccgta gttgcggtca
     2941 ttcttgaagg caatttcgtc gtcctgtgtt ttgaaagatg ggcgcgcttg cgcagggtaa
     3001 ggtgtcttgc tctgccccat ttcaccgacc cagccgtcga attcgtcgga atcgaagtga
     3061 ccgtagccat tgtagatgag cgaggcgaag ccgccgttgg cgaatgtaag aagtgcggca
     3121 taggcgccct cggtcggtct tttcgggtcc cacgctcccg tcattgcgcg taccctcgtg
     3181 accgcggcgc cggcgaccag gcgcacgatg tcgacctgat gcgccgcctg attgaagatt
     3241 gcgccgccgc cctttgacgt gtcgagctct tcgggacggc gtggccggta aagatagtcg
     3301 gtgtagttga gcgcggtgat catgcgcaca gccccgtaag caccgctctc gatcagtttg
     3361 cgcagatgcc ggaccggcgg atcgaaggaa tggctgtggc cgaccacgac ctgcacaccg
     3421 gaccggcgcg tagcctcgac gatggccttg caatccttca gcgtcagcgc catcggcttt
     3481 tcgatcaaca gatgcttgcg ggcgcgtgcc gcaagggttg cgtgctcgct gtggaattga
     3541 tgcggcgttg cgatatagac ggcgtcgacg gccggatcgg cgcagagttc cgcgatcgtg
     3601 tcgtaggcct ttgcggaaaa gtcgtcggcg aaccgcttgc gggcttccgg acgcgggtcg
     3661 gcggcggcaa tcaacgtcac cgcggggtgc cggataaatg tcggcagcat cactgaaaaa
     3721 gcgcggccga gcccggcgac gccaagccgc agcggaggag tggtcgggtc agccatcgac
     3781 aaggtccggt gctttcacta gtcttatctt agatatctaa gtaagaagac gcgacgggaa
     3841 tttctgccgc gcagaggaaa gcgccgccat gctgacttcc gaggaaaacg acctgctgtg
     3901 ccgtgtcgaa ggcaatgcgc cgatgggccg gatcatgcgt cggcactgga tcgcggcctg
     3961 cctgtcggag gaggtcgccg agcctgacgg cgcgccggtc aaggtacggc tgctgggtga
     4021 agacctcgtt gtatttcgcg acagcaaggg ccgcgtcggc gtgctcgacg agtactgctc
     4081 gcaccgccgg gcgtcgctgt tatttgcgcg caacgaggat tgcggcctgc gctgcctgta
     4141 tcacggctgg aagttcgacg tggacggcaa catcgtcgag atggcgtcgg aaccggacaa
     4201 cagcctgatc ttcgatcgcg tcaagcacaa atcctatccg acgcgcgagg cgggcgggtt
     4261 cgtgtgggcc tatatgggtc cgccggagga gatgcgcgaa ttcgagccgc cggcttttgc
     4321 gccgacgccg gacgcgcgcg tgagcgccac caaagttcgc gtgaactgca actgggcgca
     4381 gattctcgag gggcagatcg actccgcgca ttcctcacat ctgcattcgt c
//
LOCUS       DASSRL010000003         6688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_577529, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000003 DASSRL010000000
VERSION     DASSRL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577529"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..608)
                     /locus_tag="VFL49_00045"
     CDS             complement(57..608)
                     /locus_tag="VFL49_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333449.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HET7910400.1"
                     /translation="MIALNLQPAKLSPTMAAYFKKCQDKLGFVPNVLLAYAFDMAKLE
                     SFVAMYNDLMLGDSGLSRLEREMIAVAVSSQNRCYYCLTAHGAAVRQYSGNPLLGEHL
                     VMNYRVARLNKRQRAMLDFAVKLTASPWSVEEGDRERLRRVGFTDRDIWDISAVAGFF
                     NMSNRVASATDMRPNAVYHGQAR"
     gene            1043..5842
                     /locus_tag="VFL49_00050"
     CDS             1043..5842
                     /locus_tag="VFL49_00050"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HET7910401.1"
                     /translation="MAVPASSAESAARATLEKAAASLAGQRADIPAGFVEQLFGRAVP
                     EDVARYGSDDLATLAEQAYDFLIERKPGTPKIRCETITLEHSADRNSVTVVEIVNDDM
                     PFLVDSVMGELTERRLDVRLVVHPMFGVRRSGDKLAGIDAPEAAGTRESFIHIHLSPI
                     ADVACAELVHALENVLGEVRLAVQDWRPMRERVTVIIAELKATPPPLPVGEIAEAIQF
                     LQWLLADNFTFLGVRDYTVEGQALNPDFATALGVMRSREMRVLRRGNELLESTPEILA
                     FMKEPRLLIIAKANVHARVHRRVYLDYVGVKRFDASGKLVGEQRIIGLFTSTAYTRTV
                     HSIPYLRRKIAAVEERAGYDSASHSGKALANVLEHYPRDELFQIDEDTLYNFALAILQ
                     LHERPRVRVLARRDRFDRFVSVLVYVPRERYDSHIRAKIGAYLADVFIGRLSAFYPFF
                     PDEGSLVRVHFIIGRSGGATPAVERATLEQEVQSIIRTWSDALSEALALVNPPDQARE
                     LFARYRDAFSEGFHEAYAPAIAAGDIRVVEGLSEQRALGVDFHHRLEEDQRSVGLKVW
                     SLARPLPLSERVPVLENMGFRVVDERTYHIEPHDRAGVWFHDMLLTRAGGGVIDLDAG
                     KARLEAAFLMVMRGVAENDGYNALTLHGGLAWRDVALIRALSRFLRQIRVPYSQDYMW
                     TTLVKHSGIAAGIVGLFHARFDPRTEPGRSEKQSALAARLEEELQNVESLDEDRILRR
                     FINAVQSAIRTNFYQSGKDGQPKPLISVKFESRKLTDLPLPRPLYEIFVYSPRVEGVH
                     MRFGKVARGGIRWSDRPQDFRTEILGLVKAQQVKNAVIVPVGAKGGFVPKLMPKGAAR
                     DVVQSEGVATYKLFISSLLDITDNIGPDGRIIPPANVIRHEGDDPYLVVAADKGTATF
                     SDIANAISIEHDYWLGDAFASGGSAGYDHKAMGITARGAWEAVKRHFREMDVDIGKTP
                     FNVVGVGDMSGDVFGNGMLREDTIRLLAAFDHRDIFIDPDPDPKRSFAERKRLFALPR
                     SSWQDYDKALISKGGGVYPRTSKEIRLSAEARKIFGVAEAVTPQELMKAVLRAQADLL
                     FFGGIGTYIRASNESDEAAGDRANDAIRVTGAELNCKVIGEGANLGMTQRGRIEAAQR
                     GIRLNTDAIDNSAGVNTSDVEVNIKIALSAPVRAGVLSIDKRNTFLAEMTDEVARLVL
                     RNNYQQTLALSLAQRRGLEDLGFQQRLMQTLEQRGELDRPVEFLPDDMTLGERRKRNS
                     GLTRPELAVLLAYAKLSLNGELLDSKVPDDPYLGRELNRYFPREMSERYADALHAHRL
                     RREIIATQLTNSMINRGGATLIVRIADQTGAAPSSIAAAFAAVRNSYDLIELNGEIDA
                     LDNKVSGKVQLDLYAAVQSLLLDRLVWFLRNVDLAQGLEKIIGHYRDGIAQVEKALDT
                     ALSKAAASVRDGRIAELSKAGVPEALARRVANLPVLKAAPDIALVSDRAGKPVGEVTA
                     TYFATEAFFQLDSVASAVPNIVVSDYFDRLALDRALDSIGDAERRLTAAMVANGHAGA
                     DAVEEWVRPRQAEVERIRSAIHQIAGSGLTLSKLSVAASLLGDLARQ"
     gene            6153..6362
                     /locus_tag="VFL49_00055"
     CDS             6153..6362
                     /locus_tag="VFL49_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7910402.1"
                     /translation="MATGTVKFFNAQKGFGFIIQDNGGPDVFVHISAVERAGMHSLNE
                     GQKLSFDVQADPRSGKSAAANLQAA"
     gene            complement(6359..6604)
                     /locus_tag="VFL49_00060"
     CDS             complement(6359..6604)
                     /locus_tag="VFL49_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910403.1"
                     /translation="MIDVVVAHKNLQLMFLYFERQPSPEDKPIRTIRAADEKHRRRSQ
                     LVFLSDALEHFRRALDSIKDLVVLLDLHSPNDLRDGS"
     gene            6569..>6688
                     /gene="rpsU"
                     /locus_tag="VFL49_00065"
     CDS             6569..>6688
                     /gene="rpsU"
                     /locus_tag="VFL49_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7910404.1"
                     /translation="MQVFVRDNNVDHALRVLKKKMQREGVFREMKQRRSYEKPS"
BASE COUNT         1257 a         2049 c         2097 g         1285 t
ORIGIN      
        1 tggattcccg cgtgcgcggg aatgagcggg cggagaggcc tcggcgtggg aattctttac
       61 cgcgcctgcc cgtgatagac agcgttcggc cgcatgtcgg tcgccgaagc gacgcggttc
      121 gacatgttga agaagccggc cacggccgag atgtcccata tatcgcggtc ggtgaaacca
      181 acgcgtcgca gccgttcacg gtccccttct tcgacactcc agggcgaggc ggtcagcttg
      241 acggcaaaat cgagcatcgc acgctggcgc ttgttcaacc gcgccacgcg atagttcatg
      301 acaagatgct cgccgagcag cggattgccc gaatattgcc tgaccgcggc gccgtgggcc
      361 gtgaggcagt agtagcagcg gttctgcgag gacacggcga ccgcgatcat ctcgcgctcg
      421 agcctggaaa ggccggaatc gccgagcatc aggtcgttgt acatggcgac gaaggattcg
      481 agctttgcca tgtcgaaggc ataggcgagg agcacgttgg gcacgaagcc aagcttgtcc
      541 tggcacttct tgaaataggc ggccatggtg ggcgacagct tcgccggctg gagattgagc
      601 gcgatcaccg gcgcatcatc ggcacccgcc gctgcaaccg gctttgcggt ttgcttcgcg
      661 gagacaggct ttgccccagc cttgccgcct ttgatcgcgc gcagcacggg catgtctttg
      721 cccgcgttcg cgggcatcgt ctttcctgcg gctttcgccg atttgttggt cttgttgttg
      781 gtgttgggca tttccacgtc cctgacgttc gatcctgacc ccgctcaagc ctacaaggtt
      841 acatgcatgc gcgatacgat ctaacgggta ccctttcgcc ttttgtttca ggcggttaac
      901 ggccttcaat aggcctgctt tcgcgggttg tcccagcccc gtcatcaaat gccgcgccgc
      961 tctcgacgcg cctacaggga atgtgaaaag ttcaagctac ctcgaccgat tctctcaccg
     1021 cgcgtcaggc gcacgaggtt ccatggccgt tcctgccagc agcgcggaaa gcgcagcccg
     1081 cgccaccctt gaaaaagcgg ctgcgtcgct cgccgggcag cgcgcggaca tcccggcagg
     1141 cttcgtcgaa cagcttttcg gtcgcgcggt gcctgaagat gtcgcgcgtt acggatccga
     1201 cgatctcgcg acgctggccg agcaggctta tgactttctg atcgagcgca agccgggcac
     1261 accgaaaatc cgctgcgaga caatcacgct cgaacactca gccgatcgca attcggtgac
     1321 ggtggtcgag atcgtcaatg acgacatgcc gttcctggtc gactcggtga tgggcgaact
     1381 caccgaacgc agactcgatg tccgtcttgt cgtgcatccg atgttcggcg ttcgccgctc
     1441 aggcgacaag ctcgccggca tcgatgcacc ggaagccgcc ggcacacgcg agagcttcat
     1501 ccatattcat ttatcgccga ttgcggacgt cgcctgcgcc gagctggtgc atgcgcttga
     1561 aaacgtcctc ggcgaggtgc gactggcggt gcaggattgg cgcccgatgc gtgagcgcgt
     1621 caccgtgatc atcgccgaac tcaaggccac ccctccgccg ctgccggtcg gcgaaatcgc
     1681 cgaagccatc cagtttctgc agtggctgct ggccgacaat ttcacgttcc tgggtgtgcg
     1741 cgactacacc gttgaaggcc aggcgttgaa tccggacttc gcgacggcgc tcggggtcat
     1801 gcgctcgcgt gaaatgcggg tgctcaggcg cggcaacgag cttcttgagt ccacgcccga
     1861 aatcctggct ttcatgaagg agccgcggct gctgatcata gccaaagcca acgtgcatgc
     1921 gcgcgtgcac cggcgcgtct atctcgacta tgtcggcgtc aagcgtttcg atgcctcggg
     1981 caagctcgtt ggtgaacagc gcatcatcgg attgttcacg tcgacggcct acacgcgcac
     2041 ggtgcacagc attccctatc tgcgccggaa aattgccgcg gtggaagaac gcgccggata
     2101 cgattccgcc agtcattccg gcaaggcgct ggccaacgtc ctggagcact acccgcgcga
     2161 tgaactgttc cagatcgacg aggacacgct ctataacttt gcgcttgcca ttcttcagct
     2221 gcatgaacgt ccgcgcgtcc gcgtgctcgc gcggcgcgat cgctttgacc gctttgtgtc
     2281 ggtgctggtg tatgtgccgc gggaacgcta cgacagccat atccgcgcga aaatcggcgc
     2341 ctaccttgcc gacgttttca tcggccgtct gtcggccttc tacccgttct tcccggatga
     2401 aggctcgctg gtgcgcgtgc atttcatcat tgggcgttcc ggcggtgcga cgcccgcggt
     2461 ggagcgcgcg acgctcgagc aggaggtcca gtcgatcatc cggacctgga gcgacgcgct
     2521 cagcgaggcg ctggcgctgg tcaatccgcc tgaccaggcg cgtgaactgt tcgcccgtta
     2581 ccgggatgcg ttctcggagg gcttccatga agcttacgcg ccggccatcg cggccggtga
     2641 cattcgtgtc gttgaaggcc tgtccgaaca gcgcgcgctc ggcgtcgatt ttcaccatcg
     2701 gctggaagaa gaccagcgca gcgtcgggct gaaagtatgg agcctggcgc ggccgctgcc
     2761 gctgtcggag cgcgtcccgg ttctggaaaa catgggcttc cgggtcgtcg atgagcggac
     2821 ctatcacatc gaacctcacg accgtgccgg cgtctggttc catgacatgc tgctgacgcg
     2881 tgccggcggc ggcgtgatcg atctcgatgc gggcaaggca cggctggaag ctgcatttct
     2941 gatggtgatg cgcggggtgg ccgaaaatga cggctataac gcactcacct tgcacggcgg
     3001 tcttgcctgg cgcgacgtgg cgctgatccg cgccctatcg cgcttcctgc ggcaaattcg
     3061 cgtgccgtat tcgcaggact acatgtggac gacgcttgtg aaacattccg gcatcgccgc
     3121 cggcatcgtt ggattgtttc acgcccgctt cgacccgcgg acggagccgg gtcgcagtga
     3181 aaagcagagc gcgcttgccg cccgcctcga agaggagctg cagaatgtcg agagcctgga
     3241 tgaggaccgt atcctgcgtc gtttcatcaa cgcggtgcag tcggccatcc gcacgaattt
     3301 ttatcaatca ggcaaggacg gccagccgaa accgctgatc tcggtcaagt tcgaaagccg
     3361 caagctcacc gatctgccgc tgccgcgccc cctctacgag atcttcgtct attcgccgcg
     3421 cgtcgaaggc gttcacatgc gcttcggcaa ggtggcgcgc ggcggcatcc gctggtcgga
     3481 ccggccgcag gattttcgca ccgagattct cgggctggtg aaggcacaac aggtcaagaa
     3541 cgcggttatc gttccggtcg gcgcgaaagg cggcttcgtg ccgaagctga tgccgaaagg
     3601 cgcagcgcgc gacgtggtgc agtcggaagg cgtcgcgaca tacaagcttt tcatttcgtc
     3661 gctgctcgac atcaccgaca atatcgggcc ggacggcagg atcattccgc ccgcgaatgt
     3721 gattcgccac gaaggcgatg atccgtatct tgtggtcgcg gcggacaaag gcaccgcgac
     3781 attttccgac atcgccaacg caatttcgat cgagcacgat tactggctgg gcgatgcctt
     3841 cgcatcgggc ggctcggccg gatacgacca caaggccatg ggcatcaccg cgcgcggcgc
     3901 ctgggaagcc gtgaagcgcc atttccggga aatggacgtc gatatcggca agacgccgtt
     3961 caacgtggtc ggagtcggcg acatgtcggg cgatgtgttc ggcaacggca tgctgcgcga
     4021 ggatacgatc aggctgctgg cggccttcga tcatcgcgac atctttatcg acccggatcc
     4081 tgacccgaaa aggagtttcg ccgagcggaa gcggttgttt gcgctgccgc ggtcgagctg
     4141 gcaggactac gacaaggcgc tgatctccaa gggcggcggc gtctatccgc gtacgtcgaa
     4201 ggaaatccgc ctgagcgccg aggcacgcaa gatttttggc gttgccgaag ctgtgacgcc
     4261 gcaggaactg atgaaagccg tcctgcgggc gcaagccgac cttctcttct tcggcggcat
     4321 cggcacgtac attcgcgcgt cgaacgaaag cgacgaggcg gccggcgacc gcgccaacga
     4381 cgcgatccgc gtcacgggcg ccgagctcaa ctgcaaggtg atcggcgagg gcgccaatct
     4441 cgggatgacg cagcgcggtc gcattgaggc ggcgcaacgc gggatcaggc tcaacaccga
     4501 cgcgatcgat aattccgccg gagtgaacac ctccgacgtc gaagtcaaca tcaagatcgc
     4561 tctcagcgca ccggtgcggg ccggcgtgct ctccatcgac aagcgcaaca ctttcctcgc
     4621 ggaaatgacc gacgaggtcg cgcgcctggt tttgcgcaac aattatcagc aaacgctcgc
     4681 gctctcgctg gcgcagcgcc gcggcctgga ggaccttggc tttcagcaac gcctgatgca
     4741 gacgcttgag cagcgcggtg agctcgaccg gccagttgaa ttcctgcccg acgacatgac
     4801 gctgggtgag cggcgcaagc gcaatagcgg cctgacgcgg ccggagcttg cggtgctgct
     4861 cgcctacgcc aagctgtcgc tcaacggcga attgctcgac agcaaggtgc ccgacgatcc
     4921 ctatcttggt cgtgagctca accgttattt ccccagggaa atgtccgagc gctatgccga
     4981 cgcgctgcat gcgcaccggc tgcggcgtga gatcatcgcc acgcagctga ccaattcgat
     5041 gatcaaccgc ggtggcgcca cgctgatcgt gcgcatcgcc gaccagaccg gagcggcgcc
     5101 gtcatcgatc gccgccgctt ttgccgcggt ccgcaacagc tatgacctga tcgagctgaa
     5161 cggcgagatc gacgcgctcg acaacaaggt gtccgggaag gtccagctcg atctttatgc
     5221 ggccgtgcag agcctactgc tcgaccggct ggtctggttc ctgcgcaatg tggacctggc
     5281 gcagggactt gagaaaatca tcggccacta tcgggatggc atcgcgcagg tcgaaaaagc
     5341 gctcgacacg gcgctctcca aggcggccgc ctcggtgcgc gatggccgga ttgccgagtt
     5401 gagcaaggcg ggagtgccgg aggcgctggc gcgccgcgtc gccaatctgc ctgtgctgaa
     5461 agctgcgccc gacattgcgc tcgtctccga ccgcgccggc aagccggtcg gcgaagtcac
     5521 cgccacgtat tttgcgacgg aagccttctt ccagctcgac agcgtcgcga gcgctgtccc
     5581 caatatcgtc gtctcggatt atttcgatcg tctggcgctc gatcgcgcgc ttgattcgat
     5641 cggagatgcc gagcgccgcc tcaccgcggc catggtcgcc aacggccatg ccggcgccga
     5701 tgcggtggaa gaatgggtac ggccgcggca ggccgaggtc gagcgcatcc gctcggcgat
     5761 ccatcagatc gcagggtcag gcctcacgct gtccaagctg tcggtggcgg cgagcctgct
     5821 gggcgacctg gcgcggcaat agccgcgata gaggcaccgg aacgtcgacg cattgtccgc
     5881 gaagccacga tgcccgctta cggggcatag cggacatgga tcggcaggca aaaccggatg
     5941 agtcggtcga aaatgacgaa agcggacaca cacccccgat cattgcaatg cgcggccgtc
     6001 gtcgttatat gagcgatgtt cgcgattgcg cgaccttgca atttcggcct taacggccct
     6061 cctccaagac acgcaaaatc ccggatattt gagcccaggc gaatggtttg catgggtaac
     6121 gacgtctttg cgtctatata aggaggccat tgatggccac aggtaccgtt aagttcttca
     6181 acgcccaaaa gggcttcggc tttatcattc aagacaatgg cggcccggat gtgttcgttc
     6241 acatttcggc agtcgagcgc gctggcatgc acagtctcaa tgagggtcag aagctttcgt
     6301 tcgatgtgca ggccgatccg cgcagcggca agtccgctgc cgcaaaccta caagcggcct
     6361 aacttccgtc ccgaagatcg ttcggcgagt gcaaatcgag aagaacaaca aggtctttta
     6421 tcgaatcaag agcccggcgg aagtgttcga gcgcatcgct gagaaacacc agctgactgc
     6481 gcctgcggtg tttctcatct gcggcgcgaa tggttcgaat cggtttgtct tcgggtgagg
     6541 gctgtctctc aaaataaagg aacatcaatt gcaagttttt gtgcgcgaca acaacgtcga
     6601 tcatgctttg cgcgtcttaa aaaagaaaat gcagcgtgag ggtgtatttc gtgaaatgaa
     6661 acagcgacgt tcctacgaaa agccttcg
//
LOCUS       DASSRL010000004         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1662290, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000004 DASSRL010000000
VERSION     DASSRL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662290"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3282)
                     /locus_tag="VFL49_00070"
     CDS             complement(<1..>3282)
                     /locus_tag="VFL49_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HET7910405.1"
                     /translation="KDAVANDYGQQATQWFVVSDIGLTAYTTHDGIDVFIHSLATAEP
                     RGSVEVRLMARNNEQLAVRQTDRNGFIHFEAGLARGEGGLAPAAIVAAEKNDYAFLSL
                     KSPAFDLSDRGVAGRPIPAGLDAFVYTERGVYRTGESVAITALLRDAQGSAALNVPLT
                     MVVERPDGVEYKRAVVADGGLGGRSLSVPIVASASTGTWRVAAYTDPKRPPVGETTFM
                     VEDYVPDRIEFSLTSAAKGISRNAPAQVSVDGHFLYGAPASKLDLSGDVTIGVAKERA
                     GFPGYSFGLSDDEVTAVRDELDDLPGTDPAGRATFPVTLDDLPASTRPLEAQITVRMA
                     ESGGRAVERKLTLPVTPDSPMIGVKPLFSGRSLADGANAEFDVVMAAPDGKMLAKPGL
                     RYDLLRVETSYQWYRQNGQWEYEPVKRTERVANGTLDAAADKPARLSLPVKWGRYRLE
                     VSTGEPNGAITSVAFDAGFYADASADTPDLLEVALDKPDYKPGETMNVSVTARAAGRL
                     TVNVFTDRLVASQSQDVKPGAARVSLNVGRDWGTGAYMVATLRRPLDAPAQRMPGRAI
                     GVQWFSIDKLAHTLAVDMKLPATMRPNSTLSVPITLAGLTAGEEARVVVAAVDVGILN
                     LTNYKPPAPDDYYLGQRRLTAEIRDLYGQLIDGMQGVRGQIRSGGDAAGAELSGSPPA
                     QAPLALYSGIVTVGRDGAAQVQFDIPAFAGSVRVMAVAWTKEKVGKASGDVVVRDPVV
                     LTATLPRFLRTGDRGAVQLELDNVEGPAGDYNLAVVAGGAARLDGDQPQALKLAAKQR
                     DRVSIPVTASGAGPGTMTVRVSGPDNFALERNYPLDVRPATQILTRRTVRTLAKGETL
                     TLSQDMFADFVPGTGRVGLSVAVSTALDAATLINALDRYPFGCSEQIASRAMAMLYVN
                     DLAAQAQLAPDGDVDQRIKDAIPRLLARQGSNGSFGLWSVGGDDPWLDAYITDFLTRA
                     RARGFDVPQTAYMLAIDRLRNYVAAAPEPSKSGGRELAYALYVLARNGAAPIGDLRYI
                     ADVKLSDMATPIAKAQIAAALAMVGDKTRADNAYLAALDAIAPQPKLDLGRSDFGSAL
                     RDAAAL"
BASE COUNT          556 a         1097 c         1067 g          562 t
ORIGIN      
        1 agagccgccg catcgcgcag cgccgagcca aaatcgctgc gtccgagatc gagcttcggc
       61 tgcggcgcga tggcgtcgag cgccgcaaga taggcgttgt cggcgcgcgt tttgtcgccg
      121 accatcgcca gcgccgccgc aatctgtgcc ttggcgatcg gcgttgccat atcggacaac
      181 ttgacgtcgg cgatgtagcg aaggtcgccg atcggcgcgg cgccgttacg cgccagcaca
      241 tagagcgcat aggccagctc gcgcccgccg ctcttggacg gctccggcgc agcggcgacg
      301 taattccgca atcggtcgat tgcgagcatg tatgccgtct gcggcacgtc gaaaccgcgc
      361 gcccgcgccc gtgtcagaaa atcggtgatg taggcatcga gccacggatc gtcgccaccg
      421 accgaccaca ggccgaacga cccattcgag ccctgtcgtg ccagcagccg cgggattgca
      481 tctttgatgc gctggtcaac atcgccatcg ggcgcaagct gagcctgcgc ggccagatcg
      541 ttgacgtaaa gcatcgccat tgcgcggctc gcaatctgct cggagcagcc gaagggatag
      601 cggtcgagcg cattaatgag tgtcgcggca tcgagtgcgg ttgaaacggc caccgacagg
      661 ccgacccggc cggtacccgg cacgaaatcg gcgaacatat cctgcgacag tgtgagcgtc
      721 tcgcctttgg cgagcgtgcg cactgtacgc cgtgtcagga tttgcgtcgc gggccgcacg
      781 tcgagcgggt agttccgctc gagcgcaaaa ttgtccggtc cgctcaccct taccgtcatc
      841 gtgcccggtc cggcaccgga cgccgtaaca gggatggaca cgcggtcgcg ttgcttggcc
      901 gcaagcttga gcgcttgcgg ttgatctcca tcaagcctgg cggcgccgcc ggcgaccaca
      961 gccagattat agtcacctgc cggcccttcc acgttgtcga gctcgagctg cacagcgccg
     1021 cggtcgccgg tgcgcaggaa gcgcggcagt gtcgccgtca gcaccaccgg atcgcgcacg
     1081 acgacgtcgc cggacgcctt gccgaccttt tcctttgtcc aggccaccgc catcacccgc
     1141 acgctgccgg cgaaagccgg gatatcgaac tgcacttgcg cggcgccgtc gcggccgacc
     1201 gtaacaatgc cggaataaag agcgagcggc gcctgcgccg gcggcgagcc ggagagttcc
     1261 gcgccggcgg catcgccgcc cgagcggatt tgtccccgca cgccctgcat gccgtcgatc
     1321 agctggccgt agaggtcgcg aatctcggcg gtcagccggc gttggccgag gtaatagtca
     1381 tcgggcgccg gcggcttgta gttcgtgaga ttgagaatgc cgacatcaac ggccgcaacg
     1441 acgacgcgcg cttcctcgcc tgcggtcagg ccggcaagcg tgatcggcac gctcagcgtg
     1501 gagttcggcc gcatcgtcgc cggcagtttc atgtcgacgg cgagcgtgtg cgcgagcttg
     1561 tcgatcgaga accattgcac gccgatggcg cggccgggca tgcgttgtgc cggcgcgtcg
     1621 agcggccgcc gcaatgtcgc gaccatgtag gctcctgtgc cccagtcgcg gccgacgttg
     1681 aggctcacgc gcgcagcgcc cggcttgacg tcctgagact ggctggcgac cagccggtcg
     1741 gtgaagacat tcactgtcag ccggcccgcg gcacgcgcag tgaccgacac gttcattgtc
     1801 tcgcccggct tgtagtccgg cttgtcgagc gcgacttcga gcaggtcggg cgtgtccgcc
     1861 gacgcgtccg catagaagcc ggcgtcgaac gccaccgacg tgattgcgcc attcggctcg
     1921 ccggtcgaga cttccaggcg gtagcgaccc catttcaccg gcagcgacag ccgcgccggc
     1981 ttgtcggcgg cggcatcaag ggtgccgttc gcaacacgct ccgtgcgctt gaccggctcg
     2041 tattcccatt gcccgttctg ccggtaccat tgataactgg tctcgactct cagcagatcg
     2101 taacgcagtc ccggcttggc gagcattttg ccgtcgggcg ccgccatcac gacgtcgaac
     2161 tcggcgttcg ccccgtcggc gagcgagcgg ccggaaaaca gtggcttgac gccgatcatc
     2221 ggcgaatcgg gcgtaaccgg caaagtcagc ttgcgttcga cggcacgccc acccgactcc
     2281 gccatgcgta cagtaatttg cgcttccagg gggcgcgtgc tggcgggcag atcatcgagc
     2341 gtcacgggaa acgtcgccct gcctgccggg tcggtgccgg gcaagtcatc gagctcgtca
     2401 cgaaccgccg tcacctcgtc atcggacagt ccgaaactgt aacctggaaa gccggcacgt
     2461 tctttcgcaa cgccgatcgt gacgtctccg gaaagatcga gcttggatgc cggcgctccg
     2521 tagagaaaat gcccgtccac gctcacctgc gccggcgcgt tgcgtgaaat gccttttgcc
     2581 gcggatgtga gcgaaaactc aatgcggtcg ggaacgtaat cctcgaccat gaaagttgtt
     2641 tcgccgaccg gcggacgctt cgggtcggta taggccgcca cccgccatgt tcccgtcgac
     2701 gccgaggcga cgatcggcac gctcagcgag cgtccgccga ggccgccatc ggccaccacc
     2761 gcacgtttgt attcgacgcc gtccggacgc tcgaccacca tggtcagcgg cacattcaat
     2821 gccgccgagc cttgggcatc gcgcagcaat gcggttatgg cgacgctctc gccggtccga
     2881 taaacgccgc gctccgtgta gacgaaagca tcgaggccgg cggggatcgg gcggcccgcg
     2941 acgccgcggt cggacaaatc gaaggccggc gatttcaggc tcaggaaggc gtagtcattc
     3001 ttctcggccg cgacgatcgc cgcgggcgca agccccccct cgccgcgcgc caggccagct
     3061 tcgaaatgaa tgaagccgtt gcgatcggtc tgccggaccg cgagctgctc gttgttgcgc
     3121 gccatcaggc gtacctcgac gctgccacgc ggttcggcag tcgcgaggga atgaataaag
     3181 acgtcgatgc cgtcgtgggt tgtgtaggcc gtgagcccga tatccgaaac gacgaaccat
     3241 tgcgttgcct gctggccata gtcatttgcc acggcgtctt tc
//
LOCUS       DASSRL010000005         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1679793, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000005 DASSRL010000000
VERSION     DASSRL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679793"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFL49_00075"
     CDS             complement(<1..765)
                     /locus_tag="VFL49_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HET7910406.1"
                     /translation="MADYTPNEMMTIAAARALKDDDVCFVGIGMPSAAANLARLTHAP
                     NITLIYESGTLATKPNILPLSIGDGELCETAVTTVSVPEMFRYWLQGGRIKVGFLGGA
                     QIDKFANLNTTVVGPYDKPKVRLPGGGGAPEIATSCGEIFIIMAQGKRSFVDKLDFVT
                     SIGHVSGGDSRSKLGVKTKGPTKLITDLAILEPDAATKEMTVVSIHPGVTREQIQENT
                     GWKVAYATKVAETPPPTKRELDVLRDLQTRTKRAQEA"
     gene            complement(770..1630)
                     /locus_tag="VFL49_00080"
     CDS             complement(770..1630)
                     /locus_tag="VFL49_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HET7910407.1"
                     /translation="MARITSLAEAIKSAIHDGDTIALEGFTHLIPHAAGHEIIRQGRK
                     NLTLIRMTPDLVYDQLIGMGCVAKLIFSWGGNPGVGSLHRLRDAVENGWPHKLELEEH
                     SHAAMANAYEAGAAGLPCAIFRGYIGGDLPKVNSRIKRVTCPFTGEELAAVPAHRPDV
                     AVIHAQRADKAGNVMIEGVLGVQKEAVLASRRSVVTVEEIVDELPERSLNAVVLPHWT
                     IGHVVEVPGGAFPSYAQGYYPRTNSFYKEWDAISRERGSFLGWMKDNVLTKGPEVFAQ
                     HVRKFRTAAE"
     gene            1728..2180
                     /locus_tag="VFL49_00085"
     CDS             1728..2180
                     /locus_tag="VFL49_00085"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HET7910408.1"
                     /translation="MLGKQPIFTISAELEGITSLGRTPYGERRVIGILGGSVHGPKLN
                     GRIVPGGADWQIIRTDGAADIKARYIIETDDGARIMVNSEGLRHGPPEIMERLARGDN
                     VDPAHYYFRTVMRFETADAKFDWLNRILALARGQREARAVRLEVYEVE"
BASE COUNT          438 a          707 c          703 g          454 t
ORIGIN      
        1 cgcctcttgc gcgcgcttgg tgcgcgtctg caaatcgcga agcacgtcga gctcgcgttt
       61 cgtgggtggt ggagtttccg cgaccttcgt cgcgtatgcg actttccagc cggtattctc
      121 ctgaatctgc tcacgcgtga caccgggatg aatcgaaaca acggtcattt ccttcgtcgc
      181 cgcatccggt tcgagaatag ccagatcggt gatcagcttc gtcgggcctt tggtcttcac
      241 cccgagcttg ctacggctgt cgccccccga cacgtgaccg atcgaggtga caaaatcgag
      301 cttgtcgaca aagctgcgct tgccttgcgc catgatgatg aaaatctcac cgcatgacgt
      361 cgcgatctcc ggggcgccgc cgccgcccgg caaccggact ttcggcttgt catagggtcc
      421 aaccacagtc gtgttgaggt tggcaaattt gtcgatctgc gcgccgccga ggaacccgac
      481 cttgatgcgg ccgccctgga gccagtagcg aaacatttca gggaccgaaa cggtcgttac
      541 ggccgtctcg cacagttcgc cgtcgccgat cgaaagcggc aggatattcg gcttggtcgc
      601 aagcgtgccc gactcgtaga tgagcgtgat gtttggcgca tgcgtcaggc gcgcgagatt
      661 agcggcagcc gacggcatgc caatgccgac aaaacaaaca tcgtcatcct tcagcgcacg
      721 ggccgcggca atcgtcatca tttcgttcgg cgtatagtcg gccatgatct cactccgcag
      781 ccgtccggaa cttgcgcacg tgttgtgcga aaacctcggg tcccttcgtc agcacgttgt
      841 ctttcatcca accaaggaac gagccgcgct cgcgggagat cgcgtcccat tccttataga
      901 agctgttggt gcgcggatag taaccttgcg cgtacgacgg aaacgctccg cccggcactt
      961 cgaccacatg accgatggtc cagtgcggca gcacgaccgc gttgaggctg cgctcgggaa
     1021 gttcatcgac gatctcttcg accgtcacca ccgagcgcct ggaggcaagt acggcctcct
     1081 tctgcacgcc gagcactcct tcgatcatga cattgcccgc cttgtcggcg cgctgcgcat
     1141 gaatcacggc aacgtccgga cggtgcgccg gcacggcggc aagctcttcg ccggtgaacg
     1201 gacacgtcac acgtttgatc ctggaattga ccttcggcag atcgccgccg atataaccgc
     1261 ggaaaatcgc gcacggaagc ccggccgcgc cggcttcgta ggcatttgcc atcgcagcat
     1321 gcgagtgctc ctccagttcg agcttgtgcg gccacccgtt ctcgaccgcg tcgcgcagcc
     1381 gatgcagcga accgacgccc gggttcccac cccaggaaaa gatgagcttg gcgacgcaac
     1441 ccatcccgat cagctggtcg tagacgaggt caggggtcat tcggatcagc gtcaggttct
     1501 tacgtccctg gcgaataatt tcgtgcccgg cggcgtgagg aataaggtgg gtgaagcctt
     1561 ccagagcgat ggtgtcgcca tcatggatgg cggatttaat ggcttccgcg agcgaggtga
     1621 tacgcgccat agagactgga tcccggttgc gtcccgtatt tacggctggc caccgacagg
     1681 gtcaaatggc cgctggttac aagttggggc tccaacagat tgccgtcatg ctgggaaagc
     1741 aacccatttt cacgatttct gcagagcttg agggcatcac gagtctcggc cgcacaccct
     1801 atggcgaacg gcgcgtcatc ggcatcctcg gcggcagtgt gcacggccca aagctgaacg
     1861 gacggatcgt tccgggcggc gccgattggc agatcatccg cacagacggc gccgccgata
     1921 tcaaggcgcg ctacattatc gaaacggacg atggcgcccg catcatggtc aacagcgagg
     1981 gccttcggca cggaccgccg gagatcatgg agcggctggc gcggggcgac aatgtcgatc
     2041 ctgctcacta ctatttccgg accgtgatgc ggttcgaaac tgccgacgcg aagttcgatt
     2101 ggctcaaccg gattctggct ctggcacgag gccagcgcga agcccgtgcg gtgcgtcttg
     2161 aggtctatga ggtggaataa tggcggagcg tcttgcagtc aggcttccgg cctcgcttat
     2221 tgcggagcga gaaggcgcag tcgctattct gcggctcaat gcggccatcg cgcgtgaaca
     2281 tgcagcacag agcggccggc tg
//
LOCUS       DASSRL010000006         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1049987, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000006 DASSRL010000000
VERSION     DASSRL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049987"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFL49_00090"
     CDS             <1..644
                     /locus_tag="VFL49_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7910409.1"
                     /translation="VDGNIERVIARLFAVEEELPGAKPTIKQLAASLLPAERSGDFAQ
                     AMMDLGATICSPRRPACVLCPWSRACAAHARGDQETFPRKAPKREGKLRRGAAFVALR
                     ADASVLLRRRPEKGLLGGMSEVPGSEWVHNFDFDRALQFVPRIASAKWRRLPGVVRHV
                     FTHFPLELAVFVTRVPRAAKAPKGARWVRLADAPGEALPNVMRKVLAHALDGR"
     gene            complement(725..1225)
                     /locus_tag="VFL49_00095"
                     /pseudo
     CDS             complement(725..1225)
                     /locus_tag="VFL49_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            1346..1696
                     /locus_tag="VFL49_00100"
     CDS             1346..1696
                     /locus_tag="VFL49_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7910410.1"
                     /translation="MAIERHDIGPRMSKAVVHGNTVYLAGIVAENPKGKSVKEQTQDI
                     LGQIEGFLGKAGTDKSKLLSANIWITDMANFAEMNAVWDAWVAPGNTPARATVEARLA
                     APDYKVEIMAVAAK"
     gene            1699..2343
                     /locus_tag="VFL49_00105"
     CDS             1699..2343
                     /locus_tag="VFL49_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910411.1"
                     /translation="MAEQQPGVLAIWNDCRAGREGEFEAWFQGEHLQERLAVPGFLFG
                     RRHQAISGSAGYFNFYLVESPDVLTSKPYLERLDNPTPMTKRIMSEVFLNMNRTVCRR
                     TIRRGELRGAFAVTARFNETPDVDELTATLDDLVRATNVAGGEIWVALDPKGMPVSQE
                     EKLRGGDRKIKGCLMVDTLRQSDAEALGALLARHFPGAELGIFRVLCQLGRGDL"
BASE COUNT          421 a          708 c          770 g          478 t
ORIGIN      
        1 cggtcgacgg caatatcgaa cgggtcattg cgcgcctgtt cgccgtcgag gaggagttgc
       61 cgggcgccaa gccgacgatc aagcagctgg cggcgtcgtt gttgccagcg gaacgctcgg
      121 gcgacttcgc acaggcgatg atggatctcg gggcgacgat ctgttctccg aggcggcccg
      181 cctgcgtgct gtgtccgtgg agccgcgctt gcgccgcgca cgcgcgcggc gaccaggaaa
      241 cattcccgcg caaggcaccg aagcgcgaag gcaaattgcg gcgcggcgca gcattcgtcg
      301 cgctgcgtgc cgatgcgagc gtgctcttgc ggcgtcgccc ggaaaaaggc ctgctcggcg
      361 gcatgagcga ggttcccggc agtgaatggg tccacaattt tgatttcgac agggcgctgc
      421 agttcgtgcc gcgtattgca agcgccaaat ggcggcgttt gccgggcgtc gtgcgacacg
      481 tctttacgca ttttcccctc gagcttgcgg tgtttgtcac ccgagttccg cgggcggcaa
      541 aggcaccgaa aggcgcgcgc tgggtcaggc tcgccgatgc gcccggtgaa gctttgccga
      601 acgtcatgcg caaggtattg gcgcacgccc tcgacgggcg ttaaaccgcc ggaattcccg
      661 ccgccgccgc aagcatcgag ttttcagccc gctccaccgc atcaggccgc ttgcgttgtc
      721 gcgccgccgc cttgcggttg atcttgcgct tggcgattcc attcagcttt ttgtcggtcg
      781 ccttttcttc cttgaggttc gtgttcagca ctttggcaac gtcgttgcgg ccgagcagct
      841 tggcccaggc caccagcgtt ccgtatcgcg tgatctcata gtgctcaacg gcctgcgccg
      901 ctgcgattaa ggcggcgttg agcaccatct tgtcctcgat ctcgccggcg atttccttag
      961 cctcgtcgat aataccgtcg atcgcggggc actgggttcc ttgcgggctg accttcaatt
     1021 tcttgaaggc ctggtcgagc cgcttgatct gccctttggt ctgcttgaga tgcgtgcgaa
     1081 agcccttctt gagctcggca tcggaagcct tctcgaccat ctcgggcagt gccttctcga
     1141 tctgatgctc tgcgtaatag atatcctgca tgctgtgcag gaacaggtcc tgcatcgtcc
     1201 gcatgtccgg tgaaaaccaa cccatggcgc gcctccttga gtgctgttgc aagcattaag
     1261 tcgcgccgac cgtgccggtt cccacggccg ccaaagatcg gtaggaatgt cgcggttcgt
     1321 tttgagtttt tgaaagggga gtggcatggc catcgaacgt cacgacatcg gaccgcgcat
     1381 gagtaaggcg gttgttcacg gcaacacggt ctacctggcc ggcatcgttg ccgagaatcc
     1441 gaagggcaaa agcgtcaaag agcagacgca agacattctc ggacagatcg aggggttctt
     1501 ggggaaggcc gggaccgaca agagcaagct gctgtcggcg aacatctgga tcaccgacat
     1561 ggccaatttc gcggagatga acgcggtctg ggatgcctgg gtggctcccg gcaatacgcc
     1621 cgcccgcgcg acggtcgagg cgaggctggc cgcccccgac tacaaggtcg agatcatggc
     1681 ggtggcggcg aagtagcgat ggcggagcag caaccgggcg tcctggcgat ctggaacgac
     1741 tgccgcgccg gacgcgaggg cgaattcgaa gcgtggttcc agggcgagca tctgcaagag
     1801 cgcctcgccg ttccgggctt tctgttcggg cggcggcacc aggcgatttc cggaagcgcc
     1861 ggatatttca acttctatct cgtcgagtct cccgacgttc tgacctcgaa gccctatctc
     1921 gagcgtctcg acaatccaac cccgatgaca aagcggatca tgtccgaggt gttcctcaac
     1981 atgaaccgca cggtgtgccg caggacgatc cggcgcggcg agttgcgcgg cgcctttgcc
     2041 gtgacggcac gcttcaacga aacaccggat gttgatgagc tcaccgcgac gctggatgat
     2101 ctggtgcgag ccacaaatgt cgccggcggc gaaatctggg tcgcgctcga tccgaaaggc
     2161 atgccggtgt cgcaagagga gaaactgcgc gggggcgaca ggaagatcaa aggttgtcta
     2221 atggtcgata cgctgcgcca gtccgacgcg gaggcattgg gcgcactgct agcgcgtcac
     2281 tttcccggag ccgagctggg aatttttcgt gtgctctgtc agcttggacg aggtgatctg
     2341 tgaaagcgct gttgccgctc gcggaaaaaa tcgccgc
//
LOCUS       DASSRL010000007         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1399885, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000007 DASSRL010000000
VERSION     DASSRL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399885"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..498
                     /gene="rbfA"
                     /locus_tag="VFL49_00110"
     CDS             82..498
                     /gene="rbfA"
                     /locus_tag="VFL49_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7910412.1"
                     /translation="MARHKHHRESSPGGSQRQLRVGELVRHALAEMLTRGDVHDPVIE
                     GHMITIPEVRVTADLRLATIYVMPLGGRDAEEVVAAFERHKKFLRSEIARRINLKFAP
                     DIRFRVDDRFAEAERIDKLLRSPAVQRDLKHDEGGE"
     gene            576..1526
                     /gene="truB"
                     /locus_tag="VFL49_00115"
                     /pseudo
     CDS             576..1526
                     /gene="truB"
                     /locus_tag="VFL49_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            1530..1799
                     /gene="rpsO"
                     /locus_tag="VFL49_00120"
     CDS             1530..1799
                     /gene="rpsO"
                     /locus_tag="VFL49_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7910413.1"
                     /translation="MSVTTGRKAEVIKTYAKKPGDTGSPEVQVAILSERINNLTEHFK
                     SHVKDNHSRRGLLKMVGQRRQLLDYLRRTNEASYKNLIEKLGIRR"
     gene            2066..>3091
                     /locus_tag="VFL49_00125"
     CDS             2066..>3091
                     /locus_tag="VFL49_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7910414.1"
                     /translation="MFDMHRVELDWGGRKLTLETGRVARQADGAVLATYGETTVLAAV
                     VAAKQPKEGIDFLPLTVNYTEKYYAAGRIPGGYFKRERGPTEKDTLTSRLIDRPIRPL
                     FAEGWRCDTQVTVTVLSHDMENDPDIVAMVAASAALTLSGAPFMGPIGGARVGFINNE
                     YVLNPALDEMPESQLDLVVAGTNDAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIEA
                     IIKLAEKAAKEPRELTLPENAKLEKEILGLVEKDLRAAYSIARKQERQDAVAAAKAKV
                     IEHFFPDAAENQEYDKLRVAAVFKELEAKIVRWNILDTNKRIDGRDLNTVRPIVAEVG
                     VLPRTHGS"
BASE COUNT          634 a          946 c          966 g          545 t
ORIGIN      
        1 cgacttcctt cggtgaagga agaaagacgt cgatggccgg gacaagcccc ggccatgacc
       61 gattgagggt ttcaaagcgg aatggcacgg cacaagcatc atcgcgagag ttcgcccggc
      121 ggctcgcagc ggcagctgcg cgtcggcgag ctggtccggc acgcgctggc cgagatgcta
      181 acgcgcggcg acgtgcacga tcccgtgatc gaagggcaca tgatcacgat tcccgaagtg
      241 cgggttaccg ccgacctgcg gctcgcgacg atctacgtca tgccgcttgg cgggcgcgac
      301 gccgaagagg tcgttgccgc cttcgagcgt cataaaaaat tcctgcgttc cgaaattgct
      361 cgtcggatca atctgaagtt tgcacctgac atccgcttcc gggtcgacga tcgttttgct
      421 gaagccgaac gaatcgacaa gctgctgcgc tcgccggcgg tacagcgcga tctcaaacat
      481 gacgagggcg gcgagtgact gaacacaatt tgaacgatat cgatcaagct cgctccgttc
      541 ggacgccgga tgacggcgag agaccgaagc agaagcggcg cgacaagcgc gacgtgcacg
      601 gctgggtcgt gctcgacaaa cctgtcggca tgacgtcgac acacgccgta agcgtggtga
      661 agcggctgtt cagtgcgaag cgctgcggcc acgcaggcac tcttgatccc ttggcttccg
      721 gtgcgctgcc gatcgcactt ggcgaagcaa ccaaaaccgt tccattcgtt atggacggcc
      781 gcaagctcta tcgcttcacg atacggtggg gcgaagaacg cgacaccgac gatgccgaag
      841 ggcgcatcgt tgccgcgagc gcggaccggc cttcgcccga ggcggtgcgt gcgcttctgc
      901 cctcctatac ggggctcatc cagcaaatcc cgccgcgtta ctcggcgatc aagatcgagg
      961 gcgagcgggc ttacgatctg gcccgtgatg gcgaacaggt ggaacttgca gcccggccgg
     1021 ttgaaatcac tcgccttgaa ctcgttaacg gccttgaccg ggatcatgcg gtcctggagg
     1081 ccgaatgcgg caagggcacc tacgtgcgct cgctcgcccg cgatataggc cgtgccttgg
     1141 gctgttttgg ccatgtcagc gccttgcgcc gggccgcggt cgggcccttt ggcgaagaaa
     1201 ccatgatttt gctggaacaa cttgaggctc tatgccatag agccgcgtcc ggcgaggcta
     1261 gcctcgccga cgctctaatg cccgttgaga ccgcgctgga cgacatcccg gcgctggccg
     1321 tcagccgggc tgatgcggca aggctgcagc gaggccaagc cgttttgttg cgcggacggg
     1381 acgcccccaa tttccgcggc acggtttacg cgacggtttc gggccaactt ctggctcttg
     1441 ccgaactgga tcgtggcgag atcgtcccca agcgcgtgtt caacctcgcc ggcctgatgg
     1501 gcagagccgc gcgcaagaaa ggatgaccga tgtcggtaac caccggccgc aaggccgaag
     1561 taatcaagac gtatgccaag aagcccggag acaccgggtc gccggaagtg caagtggcga
     1621 tcctgtccga gcgcatcaat aatctcaccg agcatttcaa gtcccacgtc aaggacaacc
     1681 actctcgtcg tggattgctc aagatggtgg gccaacgccg tcagttgctc gattatctgc
     1741 gtcgcaccaa cgaagcgagc tacaaaaatt tgatcgagaa gctcggcatt cgccgatgat
     1801 ttgttccgcg cggggcgctt gcgcccggcg cgttccgtgg agcggcgcga ggacgcgctt
     1861 cgctcgatca agccggcggc gatgggcgcg gccggtgatt tggcaagagg cgacaaggat
     1921 cgctcttgct acaaggctag gttcgaaacg ccatggcagg atcgccagac gctgttgctt
     1981 cccgcgccgc ggtcacgcgc gacagcttct cgccgtcttg ctcatggctt tcggggctcg
     2041 ccgaaaacca tgaaagaaag acacaatgtt cgatatgcat cgcgttgaac tcgattgggg
     2101 cgggcgcaag ctaaccctcg agaccggccg cgtagcacgc caggccgacg gcgccgtgct
     2161 cgccacctac ggcgagacca ccgtactcgc cgccgtcgtg gcggcaaagc agccaaagga
     2221 agggatcgat ttccttccgc taaccgtcaa ctataccgaa aaatactacg cagccggccg
     2281 aattccaggc ggttatttca agcgcgagcg cggcccgact gaaaaggaca cgctgacctc
     2341 gcggttgatc gatcgtccga tacgcccgct gttcgctgaa ggctggcgtt gcgataccca
     2401 agtcacagtc accgtgcttt cgcacgacat ggagaacgac cccgacatcg tcgcgatggt
     2461 cgcggcctcc gcggcgctca cgctgtccgg cgctcccttc atgggtccga tcggcggcgc
     2521 gcgcgtcggc ttcatcaaca acgagtatgt acttaatccg gcactcgacg agatgccgga
     2581 aagccagctg gacctcgtgg tcgcaggcac gaatgacgcg gtgctgatgg ttgaatcgga
     2641 agcaaaggaa ttgtccgaag aaatcatgct cggcgccgtg atgtttggtc accggcattt
     2701 ccaaccggtc attgaggcga tcatcaagct cgcggagaag gcggccaagg agccgcgcga
     2761 gcttacgctg cccgagaatg ccaaactcga gaaggaaatc ctcggtctgg tggagaaaga
     2821 tctacgcgcg gcctattcaa tcgcgcgcaa gcaggaacgc caggatgccg tcgccgccgc
     2881 caaggccaag gtgatcgagc acttcttccc ggacgccgcc gaaaatcagg aatacgacaa
     2941 gctgcgtgtt gcagcggtct tcaaggaact cgaagcgaag atcgtgcgct ggaacattct
     3001 cgacaccaac aagcgtatcg acggtcgcga tctcaacacc gtacgcccga tcgtggcgga
     3061 agtcggtgtg ctgccgcgca cccatggctc c
//
LOCUS       DASSRL010000008         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_87715, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000008 DASSRL010000000
VERSION     DASSRL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87715"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFL49_00130"
     CDS             complement(<1..692)
                     /locus_tag="VFL49_00130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HET7910415.1"
                     /translation="MLLHRRELLATAAYFLAGTAIARASVISGRLPWHPNAGTPPSPA
                     RPGPWLYFSADEAAAVEAIADRFIPPDPQTPGGKDAGCGVFIDRQLAGPYGRDEGLYR
                     EGPFKKGSTQQGRQSGATIAEMYRKALAALDRHCRSTFGGKRFAELSNADKDRVLHDI
                     EDEKLKFDELDAKSFMEQILKDVQQGFFADPLHGGNRDMVAWKMIGFPGARYNYLDWI
                     DRHNQRYPLPPV"
     gene            833..1171
                     /locus_tag="VFL49_00135"
     CDS             833..1171
                     /locus_tag="VFL49_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910416.1"
                     /translation="MKTLTMIAVVAALAGGMSLAAAQNAPTSKAPISPNSINKGSEPG
                     TPSGAESQSKATGKPMHVSGKGKYCSETSAKGSLNCVYASMSSCEKHSKSSNMHCVAN
                     PKLGTTGSRH"
     gene            1321..2337
                     /locus_tag="VFL49_00140"
     CDS             1321..2337
                     /locus_tag="VFL49_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7910417.1"
                     /translation="MARASRRTKAKAGRSGFRRQGQKTARKERSIQRTIDRRDARRGK
                     PARSRGAMQAGARRYPEPKLPGQHLSKPGREAGLDLAPMYKAPFYRGSEKLRDMVALV
                     TGGDSGIGRAVAVLFAREGADVAVAYLNEDEDAEETKRAVEDEGRRCILLPGDVADPA
                     YCRNAIRKTIDAFGKLDILVNNAAFQGHANKLEDLSEEHFDRTIRTNLYGYFNMVKAA
                     VPRMKKGGSIVMTGSVTGLLGSKHLLDYAMTKGGIHAFARSLASQLVDRGIRVNVVAP
                     GPVWTPLNPADKPAKAVEKFGEDTPMGRPAQPEEIAPAYVFLASPQCSSYITGEILPI
                     VGGY"
BASE COUNT          470 a          726 c          753 g          421 t
ORIGIN      
        1 cttaccggcg gcagcgggta gcgctggtta tggcggtcga tccagtcgag gtagttgtag
       61 cgcgcgcccg gaaagccgat cattttccac gcgaccatgt cgcgattgcc gccgtgcagc
      121 ggatcggcga agaacccctg ctgtacgtcc ttcagaattt gctccatgaa tgacttggca
      181 tcgagttcgt cgaacttcag cttttcatct tcgatgtcgt gaagcacccg gtccttgtcg
      241 gcattactga gctcggcaaa acgctttcca ccgaacgtcg agcggcagtg gcgatcgagc
      301 gccgccagcg ccttgcgata catctcggcg atggtcgcgc ctgactgccg accctgctgc
      361 gtgctgcctt tcttgaatgg accttcgcga tagagacctt cgtcgcgccc ataggggccg
      421 gccagttgac ggtcgataaa cactccacaa cccgcatcct ttccgcccgg cgtttgcgga
      481 tcgggcggaa tgaagcggtc tgcgatggcc tcgaccgcgg cggcttcatc agccgagaaa
      541 taaagccacg gtccgggccg cgcaggcgag ggcggagtgc ccgcgttcgg atgccaggga
      601 agccgtccgc tgatgaccga cgcgcgggcg atcgcagtcc cggcgagaaa atacgccgcg
      661 gttgccaata gctccctgcg atgaagcagc atttgtgcaa aaccggcgcg cgcccttgcg
      721 tgcataatcg ctgcgcacgg caatggttcc tccggcaacg gtcaggaacg cctctgcgcc
      781 tttgccgttg atctgctggg cggcgacgct tcttccgcaa aggatcacag cgatgaagac
      841 actcaccatg atcgcggtcg tcgccgcgct ggcaggcggc atgtcacttg ccgcagccca
      901 gaatgcaccg acctccaagg ctccgatttc gcccaacagc atcaacaagg gatccgagcc
      961 cggaacccca tccggcgcag agagtcagtc aaaggcgacg ggaaagccga tgcacgtgtc
     1021 cggcaagggc aagtattgct cggagacatc ggccaaggga tcgctcaatt gcgtttatgc
     1081 gagcatgagc tcctgcgaga agcacagcaa gtctagcaac atgcactgcg tggccaatcc
     1141 gaagctgggt acgaccgggt cgcgtcacta aacgcgtctg cgtcactgca tcgagagggc
     1201 ggggcccgtt agccggccgc ttccgggcct tctctccgtg ttggctgcgg gaacgccggt
     1261 catggagccg agttatcgag caaggaatac gacgcacctc tcgggaatga cggggcaccc
     1321 atggccagag caagccggcg cacgaaagca aaggccggca ggtccggttt tcgcagacaa
     1381 gggcagaaga ccgcgcgcaa ggaaagatcg atccagcgca ccatcgatcg ccgcgatgcg
     1441 cgccgaggca agcccgcccg gagccgcggt gccatgcagg caggtgcgcg ccgctatcct
     1501 gaaccgaaat tgccgggtca gcatctctcc aagcccgggc gcgaggccgg cctcgatctg
     1561 gcgccgatgt acaaggcgcc gttttatcgg ggctccgaaa agctgcgcga tatggttgcg
     1621 ctggtgaccg gcggagattc cggcattggc cgcgcggtgg ccgtattgtt tgcgcgcgag
     1681 ggcgcagatg tcgccgtagc ctatctgaac gaagacgagg atgcggaaga aacaaagcgc
     1741 gcagtcgagg acgagggccg ccgctgcatt ctgttgccgg gcgacgtcgc cgatccggcc
     1801 tattgccgca acgcgatcag gaaaacgatc gatgcgttcg ggaagctcga catcctcgtc
     1861 aacaatgctg cgtttcaagg gcacgccaac aagctcgaag atctttccga ggagcacttc
     1921 gaccgcacga taaggacgaa cctttacggc tacttcaaca tggtcaaggc ggctgttccc
     1981 aggatgaaaa agggcggttc gattgtgatg accggctcgg tcaccggctt gctcggcagc
     2041 aagcacctgc tcgattatgc aatgaccaag ggcggcattc atgcctttgc gcgctcgctg
     2101 gcatcgcaac ttgtcgatcg cggcattcgc gtcaatgttg tggcccccgg gccggtgtgg
     2161 acgccgctca atccggccga caagccggca aaggcggttg agaaattcgg cgaggacaca
     2221 ccgatgggcc gtccggcgca gccggaggag atcgcgccgg cctacgtctt tctcgcctcg
     2281 ccgcagtgct cgagctacat caccggggag atcctgccga tcgtcggggg gtattgaatc
     2341 gctggaacgg caaaggttta cggcgtttcg
//
LOCUS       DASSRL010000009         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1155019, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000009 DASSRL010000000
VERSION     DASSRL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155019"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..589)
                     /locus_tag="VFL49_00145"
     CDS             complement(164..589)
                     /locus_tag="VFL49_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7910418.1"
                     /translation="MPLSDTIKTLIRNAWDDGYPCLVATNGPQGPNITPKGSMIVFDD
                     EHLAWWERSKRAVLENLGHDKRVCVMYANFKAQRDGVLDSGFLRFFGSVALHETGPLR
                     EAIFAKLLPREQTHAGAEAGIGVLIRIEKAIDVRGKPIM"
     gene            713..1024
                     /locus_tag="VFL49_00150"
     CDS             713..1024
                     /locus_tag="VFL49_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HET7910419.1"
                     /translation="MSEPLVVVIPHRLGKDEALRRIKDGLGRAKGEFAWLLSIDQDVW
                     TGDRLSFRASALGQTATGFIDVYEAGVRVEVTLPWLLARFAHAAQRVIGQKGQLMLEK
                     K"
     gene            complement(1098..1430)
                     /locus_tag="VFL49_00155"
     CDS             complement(1098..1430)
                     /locus_tag="VFL49_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7910420.1"
                     /translation="MRRGLVIVVSTLILLADSAHALDLNSFRAQHGRRPLSPSGALSG
                     AAYSHAQSLAGRGRLDHAGFRQRVSIERGTAAENVAFGCETEDCVIRMWARSGRHRAN
                     MLRRDVTS"
     gene            complement(1440..1703)
                     /locus_tag="VFL49_00160"
     CDS             complement(1440..1703)
                     /locus_tag="VFL49_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910421.1"
                     /translation="MRNRALLVAALSLALTSCVGVYGPKGNDTGGIIPWSPDAERDAL
                     NIAQANCSQFNKYAVITSVHRVYGDYIAYACWWHPPRGKRHRS"
     gene            1998..>2603
                     /locus_tag="VFL49_00165"
     CDS             1998..>2603
                     /locus_tag="VFL49_00165"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7910422.1"
                     /translation="MLALFGDIMRHLFLPACICLMTLSGASGADEIPSGPLSTTKAEE
                     SKAPATVADTAADEAAVQVDSGELKSETTETLPVAVDVPLPVKRPPPIKDRVVARSRH
                     EICATVIESAHSNDLPLPFFIHLLFRESGFKPDVVSRAGAQGIAQFMPETAATVGLNN
                     PFDPIQAIAASARLLGNLVQQFGNLGLAAAAYNAGPRRIQDW"
BASE COUNT          474 a          815 c          835 g          479 t
ORIGIN      
        1 tgtgggccaa tctcgattca aagatctatc acttcagcgg caacaagacc tacgggcaga
       61 ccaaatccgg cgcgtacatg tgcgagcggg atgcgatgag ccagggcatg cgggccgcga
      121 agaacgagaa gcatccctga aatcagatcg tccgcggcgc cggtcacatg atcggcttgc
      181 cgcggacgtc aatcgccttt tcaatgcgga tcagcacgcc gatgccggcc tccgcgccgg
      241 cgtgcgtttg ctcgcgcggc aacagcttcg cgaagatcgc ttcgcgcagc ggcccggttt
      301 cgtgcagcgc gaccgagccg aagaaacgca agaaaccgga atccagcacg ccgtcgcgtt
      361 gcgctttgaa gttcgcgtac atcacgcaga cgcgcttgtc gtgaccgagg ttttccaaga
      421 cggcgcgctt gctgcgctcc caccaggcca gatgctcgtc gtcgaagacg atcatgctgc
      481 ctttcggcgt gatgttcggt ccttgcggtc cgttggtcgc gacgaggcac ggatagccgt
      541 catcccaggc gttgcggatc agcgtcttga tcgtgtcgga gagaggcatg cggcgtttcc
      601 ctgttcggcg ccgagcttaa gataaatcgc cggaacctga catccggtca gcgcgtccga
      661 gccttccgcc gcgtgggcga catccccata tttccgcgag agggagaacc caatgtcaga
      721 gccgctcgtg gtggtcatcc cgcatcgcct cggaaaggac gaagcgctgc gccggatcaa
      781 ggacgggctc ggccgcgcca agggcgaatt cgcctggctg ctgtcgatcg accaggacgt
      841 ctggaccggt gaccggctgt cgttccgtgc ctccgctctg ggccagacgg cgacaggctt
      901 catcgatgtc tacgaagccg gcgtccgggt tgaggtcacg ctgccctggc tgctcgcgcg
      961 ctttgcccat gccgcgcagc gcgtgatcgg ccagaagggc caactgatgc ttgaaaagaa
     1021 gtagggcgtg cccgtcagtt cccgagctcg agcacccaat agcgccggcc gttgctgccg
     1081 acggccgaag caatccccta gctcgtcacg tctcgccgca gcatgttggc gcgatggcgg
     1141 cctgatctcg cccacatgcg gatgacgcaa tcttccgtct cgcagccaaa ggcaacgttt
     1201 tccgcagcgg tgcctcgctc gatcgaaacg cgctgacgga aaccggcgtg gtcgagccgg
     1261 ccgcgcccgg caaggctctg cgcatggcta taggcggcac ccgacagcgc gccgctcggc
     1321 gacagcggcc ggcggccatg ctgggcgcgg aatgaattga gatccagtgc gtgtgcagaa
     1381 tccgcaagga gaatcaaagt cgaaacaacg atgaccagcc cgcgccgcat gaggcgaggc
     1441 tacgaacggt gccttttgcc gcgtggcggg tgccaccagc aggcataggc gatgtagtcg
     1501 ccgtaaacgc gatgaacgct ggtgatcacc gcgtacttgt tgaattggct gcagttggct
     1561 tgcgcgatgt tgagcgcgtc gcgctcggcg tccggggacc agggaataat gccgccggta
     1621 tcgttgccct tcgggccata gacgccgacg catgaggtaa gcgccaggct cagcgccgcc
     1681 acaagaagtg cccggtttct catttggatt tccccgcccg ccaaagcctc aaccgactca
     1741 atgctaagtg gtcgctgcgc gatataccag cccttgcctg aacaggcgat aaaatggccg
     1801 cgttgtggct tggttcgggg cgcacggggt gaggcgattc ggcaaggaac tgcggcaatc
     1861 ccggggcgga tttgctctta ttctctccac acagtctcca aaaatcggtc ccgtttcaac
     1921 cgcatcaccc ggctcacgcg aggggacctt tccttttcga aatcagcgtc ggcgcattgg
     1981 gatgcgcgcg cgagggcgtg ctcgctctgt tcggggacat tatgcggcat ctgtttctgc
     2041 ctgcctgtat ctgcctgatg actttgtcgg gcgccagcgg cgcggacgaa attccgtccg
     2101 ggccgctttc cacgaccaag gccgaagaga gcaaggcgcc ggcgacggtg gccgacacgg
     2161 cggccgatga agccgctgtg caggtcgaca gcggcgaact gaaaagcgag acaacggaga
     2221 ccttgccggt cgcggtcgat gtgccattgc cggtcaagcg cccgccgccg ataaaggatc
     2281 gcgtggtggc ccgctcgcgc cacgagatct gcgcgaccgt gatcgagtcg gcgcattcga
     2341 atgatctgcc gctgccgttt tttattcatc ttctttttcg ggaaagcggc ttcaagcctg
     2401 acgttgtgag ccgcgccggc gcgcagggga ttgcgcaatt catgccggag acggcggcga
     2461 ccgtcggtct caacaatccc ttcgatccga ttcaggcgat cgcggcgtcc gcgcgcttgc
     2521 tcggcaatct cgtgcagcag ttcggaaatc tcgggcttgc cgcagccgcc tacaatgccg
     2581 gaccgcggcg catccaggac tgg
//
LOCUS       DASSRL010000010         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_840108, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000010 DASSRL010000000
VERSION     DASSRL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840108"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1234
                     /locus_tag="VFL49_00170"
     CDS             314..1234
                     /locus_tag="VFL49_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910423.1"
                     /translation="MGPSVRTLLTSFLIAAVAPVGMAILDLDPPAFAKNGGGNGGGHG
                     GGNGGGHGNGHGGGNSGNHGNANGHAKTADTDTATDSDIGTVADTKSHKSKAGLTGED
                     SLSPSALGKLNGVLHASPNAIAHASINSPIGTARAFGEALAGFLDAAATDDTTDDTTD
                     DTTGEETTTGDETQPDIDQLGEMMARMTNKPVTAEQVEAVAAKLGIETAPEEATGDET
                     TGDETTDGETVGDETSTDEPTDQPEAPKLDQATAEAIAEKANEIHGFTDGTDTTDDTD
                     TTDDTDGDAADAGDGTDGGDTGGTDETVTN"
     gene            complement(1297..2802)
                     /locus_tag="VFL49_00175"
     CDS             complement(1297..2802)
                     /locus_tag="VFL49_00175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7910424.1"
                     /translation="MTFEDLGLSPSILQAIADLGYQQPTPIQAQAIPVVLMGRDVLGC
                     AQTGTGKTAGFTLPMIEILSSGRARARMPRSLIIEPTRELAAQVADSFEKYGKNANLS
                     YALLIGGESFVDQEKKLDRGVDVLIATPGRLLDLFERGKILMNDVKILVIDEADRMLD
                     MGFIPDVERIVGLLPKLRQTLFFSATMAPEIRKLADAFLMNPKEISVSPPATAPTTVI
                     QGIAVVEMEQKREALRRLIRSENVTNALIFCNRKRDVEILFKSLTKHGFDAVRLHGDM
                     SQPARTETLDKFKRGEARLMVCSDVAARGLDIKGLSHVFNFDVPMHSEDYVHRIGRTG
                     RAGREGHAFTLAAPIDGKYVAAIEKLLGTTIPRITIEGLESAALSSGRDHRRGGRHDR
                     AHQQQEKRQRKAAEVIDAVVPHPELEGGRHAMLEASVALPQLEPEQQVNRHHRRAGGR
                     DHQEPQQHSNGRERRAPRRDYRGQDEGLPGVAFGDHTPAFLLRRGRSGEAR"
     gene            complement(2978..3505)
                     /locus_tag="VFL49_00180"
     CDS             complement(2978..3505)
                     /locus_tag="VFL49_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910425.1"
                     /translation="MSRLLAIILIGALAAGCTPGRIDRVRVEDGYDPVQYQFVPYTRA
                     MRADVSGNPFGMDQEAFNNLVSDTIQPRGVLASDGSPYRVHLAFNGSTSVNRDLACRA
                     SGGGASHGDMTLVAALCPGDGPALTFLTGSVSDITGPDDPRFHQFVRSSVVKLFPGPT
                     IDNRNQNDRCFVPMC"
     gene            3585..4040
                     /locus_tag="VFL49_00185"
     CDS             3585..4040
                     /locus_tag="VFL49_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HET7910426.1"
                     /translation="MTVHLIKLCVGVSDVSQLLQEHERLTRQALKRKKSIEDHHWTRM
                     RPKRAEEVIEGGSLFWVIRGIIRVRHRILRLDHVRDADGQEYCAIIYDPRVILVEPRP
                     RRAFQGWRYLEVEDAPPDLPNQRQALANAAAEGDMPPQMLAELRILGLF"
     gene            4564..>4701
                     /locus_tag="VFL49_00190"
     rRNA            4564..>4701
                     /locus_tag="VFL49_00190"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          897 a         1403 c         1509 g          892 t
ORIGIN      
        1 cggtggacag caaagttgcc ggggcatgcg atcccatccg caaagctgag atgcaggcga
       61 tgcgctctct gatctgggct aggtcaatcc gggcattcat ttcgcttggc ttagactgta
      121 tgttctcttt ttgttctaaa cactccaagc tgtcaagaca gtcgctaaac tccccgaaat
      181 ctcactaaat gttttcaaaa aaacacaata aaatgaatga attaaattcg tttttgcccg
      241 ccccggttca tgcgccattc aggcatccgt tgctagcgtc ggaccaccgt ctgaacaaag
      301 tcaggagcac gatatgggcc cctcggtccg tactcttctt acctctttcc tcatcgcggc
      361 ggtggccccg gttggaatgg caattctcga cctggacccc ccggctttcg ccaagaacgg
      421 cggagggaac ggaggcggcc atggcggcgg caatggcggt ggccacggta atggtcatgg
      481 cggtggcaat agcggcaacc atggcaatgc caatgggcat gccaaaaccg ccgacaccga
      541 tacggctacc gacagcgata tcggcactgt cgccgatacc aagtcccaca agtccaaggc
      601 tggcctgacc ggcgaggatt cactgtcgcc cagtgccctt ggcaagctca acggcgtcct
      661 gcacgcttct ccgaacgcaa ttgcacatgc cagcatcaac tcgccgatcg gcacggcccg
      721 ggcattcggc gaagcgctcg cgggtttcct ggacgctgcc gcgaccgacg acaccaccga
      781 tgatacgacg gacgacacga ccggcgaaga gaccaccacg ggcgatgaaa cacagccgga
      841 tatcgaccaa ctcggcgaaa tgatggccag aatgaccaac aagccggtca cggcggagca
      901 ggtcgaagcg gtcgccgcga aactcggcat cgagaccgca cccgaggaag cgaccggcga
      961 cgaaaccacc ggcgacgaga cgaccgatgg tgaaacggtc ggcgatgaga ccagcaccga
     1021 tgagcctacc gaccagcccg aagcgccgaa gctggatcag gcaacggccg aggcgatcgc
     1081 cgagaaagcc aacgagatcc acggctttac ggacggcacg gatacgaccg acgatactga
     1141 cacgaccgat gacaccgacg gcgacgccgc cgatgccggc gacggcaccg atggtggcga
     1201 taccggcggc accgacgaga cggtcaccaa ctagaacgct tcaaacttgc ggaacacctc
     1261 agcgcgggag acttctcccg cgcttcttct ttgcgctcag cgcgcttcgc cggaccgacc
     1321 gcggcggagc aggaaagccg gcgtgtgatc gccgaaggcg acgcccggca agccttcatc
     1381 ctggccgcga tagtcgcggc gcggcgcgcg acgctcacga ccattggaat gctgctgcgg
     1441 ctcctgatgg tcgcgcccgc cggcccggcg gtgatggcgg ttgacttgct gctcgggctc
     1501 gagctggggc agcgcgacgc tggcttccag catggcgtgc cggccgcctt caagctcggg
     1561 atgcggcacc accgcatcga tgacctcggc ggccttgcgc tggcgttttt cctgctgctg
     1621 gtgcgcccgg tcatggcggc cgcccctgcg atgatcccgg ccggaagaca aggccgcgct
     1681 ctccagccct tcgattgtga tgcgcggaat cgtggtgccc agcagcttct cgatcgccgc
     1741 cacatacttg ccgtcgatcg gcgcggcaag ggtgaaggcg tggccctcgc gcccggcgcg
     1801 gccggtgcgg ccgatgcgat gcacgtaatc ctcggaatgc atcggcacgt cgaaattgaa
     1861 gacatggctg aggcccttga tgtcgaggcc gcgggcggcg acatcgctgc acaccatcag
     1921 acgcgcctcg ccccgcttga acttgtcgag cgtctcggtg cgtgccggct gcgacatatc
     1981 gccatgcagg cggaccgcat cgaagccgtg cttggtgagc gacttgaaca ggatctccac
     2041 atcgcgcttg cggttgcaga agatgagcgc gttggtgacg ttctcgctgc ggatcagccg
     2101 gcgcagcgcc tcgcgcttct gctccatttc gaccaccgca atgccctgga tcaccgtggt
     2161 tggcgccgtc gcgggcggag aaacgctgat ctccttcggg ttcatcagga atgcatccgc
     2221 cagcttgcgg atttccggcg ccatggtggc ggagaagaac agggtctggc gcagcttcgg
     2281 cagcaatccg acgatccgct cgacgtccgg gatgaagccc atgtcgagca tacggtcggc
     2341 ttcgtcgatc accaggatct tgacgtcgtt catgaggatc ttgccgcgct cgaacaggtc
     2401 gagcaggcgg cctggcgtcg cgatcagaac gtccacgccg cgatccagct tcttctcctg
     2461 gtcgacgaag ctttcgccac ctatgagcag cgcgtagctc aggtttgcgt tcttgccata
     2521 cttctcaaag ctgtcggcaa cctgcgccgc cagctcccga gtcggctcga tgatcagcga
     2581 gcgcggcatg cgggccctgg cccggccgct gctcaggatc tcgatcatgg gaagggtgaa
     2641 gccggcagtc tttccggttc cggtctgggc gcagcccagg acatcccggc ccatgagaac
     2701 cactggaatt gcttgggctt ggatgggggt tggctgctga tagccgaggt cggcgatcgc
     2761 ctgcaggatg gaggggctta gccccagatc ttcgaacgtc attatctcaa gatgcgggcg
     2821 tttaccggga tcgccgaagg gcgaaagtgg gcagggggag cccgatcccg ctgccggaaa
     2881 gtcgcgcgca ctatagggac aaggcaagct ttgtcaattc aaccggttgc gcgctacttt
     2941 gcagctaagc caaattgcat agctcaattg gcggcaatca acacatcggc acgaagcagc
     3001 ggtcgttctg gttgcggttg tcgatcgtcg ggccggggaa caacttcacg acggaagacc
     3061 ggacgaattg gtggaatcgc ggatcatcgg gccccgtgat atcggagacc gatcccgtca
     3121 ggaacgtcag cgccggcccg tcgccagggc acaaggccgc gacgagggtc atatcgccgt
     3181 gcgaggctcc gccgccggag gcccggcagg cgaggtcgcg attgacgctg gtcgatccgt
     3241 tgaaggccag gtgcacccgg taagggctgc catcgctcgc cagcacgcca cgcggctgga
     3301 tggtatcgga aaccagattg ttgaaggcct cctggtccat gccgaagggg ttgccggaaa
     3361 cgtcggcccg catggcccgc gtatatggga cgaactggta ttgcacggga tcatacccgt
     3421 cctcgacgcg gacgcggtct atccgccccg gcgtgcagcc ggctgccaga gcgccgatca
     3481 gaatgattgc gagaagcctg gacatcgtcc tctcctcagg gaatctgttc aattctattt
     3541 gacgaccgat cgtccgagaa ccaatcatca cgatttcgcg agacatgacc gttcacctca
     3601 tcaagctctg tgtcggcgtc agcgacgtca gtcaactgct ccaggagcac gagcgcctga
     3661 ccagacaggc cctgaagcgg aaaaagtcga tcgaggatca ccattggacg cgcatgcggc
     3721 caaagcgggc tgaagaggtg atcgagggcg gttcgctctt ctgggtcatc cgcggcatca
     3781 tccgtgtccg acatcgaatt ctgcgcctgg atcacgtgcg ggatgctgat ggccaggaat
     3841 attgcgcgat catctatgac ccgagggtga tcctggtcga gccgcggccg cgccgcgcct
     3901 tccagggttg gcgatatctg gaagtcgagg atgcgccgcc ggatctgccc aatcagcgcc
     3961 aggcgctcgc caacgcggcg gcggaagggg atatgccgcc gcagatgctc gcggagctgc
     4021 gcatcctcgg cttgttctag gtgactccgc cgcgtgcaga ttggccggat gttgatggca
     4081 cggggtgtgg ataagattgt tgcgttttcc cctttacacc ccgtgcggac tcgactagaa
     4141 accgcgccgt cgaacggagc cgctggttct tggtcggctc cggaaacagc agcaaaaatc
     4201 ggctgttggt tgaacgggcg atgcgctctt ggagtgcttg aatcgcccat ccggcatcgc
     4261 tatattgcgc gtttgccttt ggccgaatca ggtcgaggcg cggcgtgtgc ggcgccctgc
     4321 tatttgtcat tgttgttatg attgagaagg gattcgcggg cggcgggttt tgggttggta
     4381 tccggagctt ttgctgtttc gtggatcttg ggtctggttg ttgttggtat ctgctgtgcg
     4441 gagcgatctg cgtggcgagg tcttgcgatg atcggatctg gtgagacgcg ttatgtgcag
     4501 aggcgaattg tcagttggtt tgcctggctt gattgatctt cggattgatt gggtttggcg
     4561 gattaacctg agagtttgat cctggctcag aacgaacgct ggcggcatgc ctaacacatg
     4621 caagtcgaac gggatccgga cttcggttcg gtgagagtgg cggacgggtg agtaacgcgt
     4681 gggaacctgc ccagtggtgg g
//
LOCUS       DASSRL010000011         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_297679, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000011 DASSRL010000000
VERSION     DASSRL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297679"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFL49_00195"
     CDS             <1..265
                     /locus_tag="VFL49_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HET7910427.1"
                     /translation="EAIRRIRQGFARGEGPLGPMIRMEQPTWEGDTLRFVMRALGQSA
                     AAKVDVFDDALRIEVSLPWLLAKAAKRLVPILRKEATLLLEKK"
     gene            complement(282..686)
                     /locus_tag="VFL49_00200"
     CDS             complement(282..686)
                     /locus_tag="VFL49_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910428.1"
                     /translation="MSPRHTARRFLALATAACRSRGIACLAATLFVGATVSHAQPAPG
                     PAASAPAAVPQQIPAPTDPQTLSCNDLKARLHTTGQLPILVGPRGGWADTFYGPQVPR
                     CQFWQMPWFQYVRTNDGLCGVGYICIDKLSFD"
     gene            907..1458
                     /locus_tag="VFL49_00205"
     CDS             907..1458
                     /locus_tag="VFL49_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910429.1"
                     /translation="MTARNWIHIVALSSAFGLAGAAIAQNTEGTTSTEDRAGMTSPGA
                     ETPSAGAIVTPEDKARGMGQDSERSGILDNGVDESAPGSDDDASGSSPAPLNGDDSSA
                     NPGASEDQQMGIDPGPSSDDESSADPGLSDDDELSINPGPTEDDESSVNPGLSADEEL
                     SVNPGLSSRDEASRNTSSTVRTQ"
     gene            1673..>2634
                     /locus_tag="VFL49_00210"
     CDS             1673..>2634
                     /locus_tag="VFL49_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HET7910430.1"
                     /translation="MSDQPQQPGKPDRDEKAHARLVSQVRMMLAAAWASPVRNTLLWL
                     AGATFLVIVATAYGQIRLNRWNQPFYDALARRDVSEFVVQLGVFAVIAGVLLVLNVCQ
                     RWLGETIKLKLREGLTRDLIDLWMLPRRAFRLANAGPIGVNPDQRIHEDARHLTELSA
                     DLGIGLLQAAILLATFVSVLWKVSADFAFVVEGRVVAIPGYMVWAGLLYAGSASLLSY
                     WVGRSLVGRNAERYAREAELRFSLVRVNEHIDAISLAGGEAEEARRIKLDLAAVLSAM
                     RALVIGHTNLTWVTAGYGWLTIVAPILVAAPLYFGGNVTFGGLM"
BASE COUNT          497 a          822 c          867 g          448 t
ORIGIN      
        1 cgaggcgatc cgccggatca ggcaagggtt cgcgcgcgga gaagggccgc tcggaccgat
       61 gatcaggatg gagcagccga cctgggaagg cgatacgctg cgcttcgtga tgcgcgcgct
      121 cggtcaaagt gctgcagcca aggtggacgt gttcgacgat gcgctgcgca tcgaggtgtc
      181 gctgccgtgg ctgctcgcaa aagcggcaaa acgcctggtt ccgatactgc gcaaggaagc
      241 gacgctgctg ctggaaaaga aataggcgct tcttttccgc ctcaatcgaa cgatagcttg
      301 tcgatgcaga tgtaaccgac gccgcacagg ccgtcgttcg tcctgacgta ctgaaaccag
      361 ggcatttgcc agaactggca ccgaggtacc tgcgggccat agaacgtatc tgcccaaccg
      421 cccctgggcc cgacgaggat cggcagttgc ccggtcgtgt gcagccgagc cttcaagtca
      481 ttgcaggaca gtgtttgcgg atcggtgggg gccggaattt gctgtgggac tgcagcaggt
      541 gcactggcgg ctggtccggg cgccggttga gcgtggctca cagtcgcgcc gacgaacagg
      601 gtcgcagcaa ggcaggcaat gccccggctg cggcaggcgg cggtagccaa tgcaagaaag
      661 cggcgagccg tgtgtcttgg cgacatgatg acgtcctcgg aaagagggat tttgacgcta
      721 acactccagc tgtaagtctt gttccaatga cgtaaacccc cgcttgcctt cacaagcgcc
      781 tacaaccgaa tgcggaccgc cccggcagtc tggggccggg ctacccggga aaatggacgc
      841 atgcgcttgc gatacggcct tttgggcctg ccagcgcggg catccacggc ttatgaagga
      901 gagagcatga cagcaaggaa ttggatacac atcgtcgcac tgagttcggc tttcgggctc
      961 gccggcgccg cgatcgcgca gaacacggaa ggcacgacga gcaccgaaga ccgtgccggc
     1021 atgacgagcc ccggcgccga gacgccctcc gcaggtgcca tcgtgacccc cgaggacaag
     1081 gcgcgcggaa tgggtcagga cagcgagcgc agcgggatcc tcgacaatgg cgtcgatgaa
     1141 agcgcacccg gatcggacga tgatgcgtcg ggctcgagcc ccgcgccgtt gaacggtgat
     1201 gactcgagcg ccaatcccgg ggcctcggaa gaccaacaga tgggcatcga tcccgggcca
     1261 tcgagcgacg atgaatcgag cgctgaccct gggctctccg atgacgacga gttgagcatc
     1321 aatcccgggc ccacggaaga cgacgagtcg agcgtcaatc ccgggctctc ggccgatgaa
     1381 gagctgagcg tcaatcccgg attgtccagt cgcgacgaag caagcagaaa tacatcgtca
     1441 accgtgcgca cgcaatgaac aagcaaggag tgttcggcgg aactcaatag caatccgaag
     1501 ttcggcagca acaacgcgaa gttcggcagc aacaacggcg gcgttccacg tcgccgtttt
     1561 tttgggtagt cgcctcgcgc ggaacgcggt cgcccatccg attgccttgc tcgtgccctg
     1621 actgccgccc cggcaagtag actggctgta gcgatcgaaa tcggggcgac tcatgagcga
     1681 ccagccgcaa caaccaggca agcccgaccg cgacgaaaag gcccacgcgc gactcgtctc
     1741 gcaggtccgc atgatgctcg cggccgcgtg ggcgtcgccc gtacgcaaca cactgctgtg
     1801 gctcgcgggc gccacctttc tggtcatcgt cgcgaccgcc tatgggcaga tccggctcaa
     1861 ccgctggaac cagccgttct acgacgcgct ggcgcgacgc gacgtttcgg agttcgtcgt
     1921 gcagctcggc gtcttcgccg tcatcgcggg cgtgctgctc gtgctcaacg tctgccagcg
     1981 gtggctgggt gaaacgatca agctcaagct gcgcgaaggc ttgacccgtg acctgatcga
     2041 cctctggatg ctgcctcgcc gtgcctttcg cctcgcgaac gccgggccta tcggcgtcaa
     2101 tcccgaccag cggattcacg aggatgcgcg acacctgacc gagttgtccg ccgacctcgg
     2161 catcgggctc ctgcaagccg ccatcctgct cgccacgttc gtaagcgtgc tttggaaggt
     2221 gtcagccgac ttcgccttcg tcgtcgaagg tcgggtggtt gccattccag gctacatggt
     2281 ctgggcgggg cttctctacg cgggctccgc ttcgctgctg agctattggg tcgggcgcag
     2341 cctggtcgga cggaacgcgg agcgctatgc acgcgaagcc gagcttcgtt tttcgctggt
     2401 gcgcgtcaac gagcacatcg atgccatctc gctcgccggc ggcgaggcgg aggaggcgcg
     2461 gcgcatcaag ctcgacctcg ccgcggtgct ctccgcaatg cgcgcgctgg tcatcggcca
     2521 caccaacctg acgtgggtga cggcgggata cggatggctg acgatcgtcg cgccgatcct
     2581 cgtggcggcg ccgctctact tcggcgggaa cgtgacgttc ggcggcctca tgat
//
LOCUS       DASSRL010000012         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1609995, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000012 DASSRL010000000
VERSION     DASSRL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609995"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..861)
                     /locus_tag="VFL49_00215"
     CDS             complement(130..861)
                     /locus_tag="VFL49_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET7910431.1"
                     /translation="MTPASVSSTVRGGIIAAGEGSRLRADGYRVSKPMVPVGGRPLID
                     HALDRFRSAGIRRLTIVINEASDDCRQWLKDHAGDFDLDMIVRSTPSSYVTFRLVADR
                     LADGPAVVTTVDAILPVDQFRTFATSASQFPDDAVVLGVTGHVDDENPLWVTLDTSGG
                     RIRQIGGSNGTHVTAGLYWLPAKRPAAPATDFARLRDYLGALVAGDEPIYGIVLPCVF
                     DIDRARDVAAAESAVFGPQREKSAP"
     gene            complement(858..1544)
                     /locus_tag="VFL49_00220"
     CDS             complement(858..1544)
                     /locus_tag="VFL49_00220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7910432.1"
                     /translation="MNAPIKTLLFDFGGTLDADGVAWKERFHALYRAQGLNLDGEAFA
                     PAFHAADDPLVGGLPSTMDLSGTVHALTANLESELARRAGNGSGDRGRRIAAGFLSEA
                     VATFERNRPMLEALSERYRLGIVSNFYGNLEAVCESAGLCSFFEVMVDSHCVGAEKPD
                     PAIFRAALDRLAATPEETVFIGDSLRRDREGARRLGMRFIWVAPQDVQAEQSGQQAVT
                     DLRDLTKFLQ"
     gene            complement(1620..>2735)
                     /locus_tag="VFL49_00225"
     CDS             complement(1620..>2735)
                     /locus_tag="VFL49_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET7910433.1"
                     /translation="VGNCASSLVQGVEYYRDRQGAELEGVMRHLIGGYRCSDVEFVAA
                     FDVDRRKVGHPLEEAIFAKPNCTRIFQSALPVSNVIVQAGPLLDGVAAHMADYPDDSA
                     FRPVDLEPTDVAAALRASGAEVLVCYLPVGSERAVKHYAQACLDAGVAMVNCVPVFLA
                     SDEVWGGKFRDAGLPIIGDDIKSQVGATIVHRALARLFADRGVGLDRTYQLNTGGNTD
                     FLNMLEKSRLKSKKVSKTESVQSQLDERLDARDIHIGPSDYVPWQQDNKICFIRMEGR
                     GFGNAPIELELRLSVQDSPNSAGVVIDAIRCVKLALERGEAGPLEAPSAYYMKSPPRQ
                     LRDSVALDACNAFIEGHRFDLPGEEPNAKLASSPQRS"
BASE COUNT          537 a          882 c          837 g          479 t
ORIGIN      
        1 gctcgacgtt gaaaccgcgc gacgtcatcg cctcgccgac gcaatccatg atggcgcgat
       61 catcgccgac gcggcccggc gaatgatccg gctctctgta gatgcccttg caggatttgg
      121 ccaatgcgct catggcgcgg acttctcgcg ctgaggacca aaaaccgcgc tttcggcggc
      181 cgcgacatcg cgcgcgcggt cgatgtcgaa gacgcagggc aggacgatgc catagatcgg
      241 ttcgtccccc gcaaccagcg caccaaggta atcgcgcaac cgcgcgaagt ccgtcgcagg
      301 agcggcgggt cttttggccg gcagccaata gaggccggcg gtcacgtggg taccgttgct
      361 tccgccgatt tggcggatac gcccgcccga agtatccaat gtgacccaca gcggattttc
      421 atcgtcgaca tggccggtca cgccaagcac caccgcatcg tctggaaact gcgaggccga
      481 tgtcgcaaag gtccggaact ggtcgacagg caggatcgcg tcgaccgtcg tgaccacggc
      541 gggtccatcg gccagccggt ctgcgacaag ccggaaggtg acgtaagatg acggcgttga
      601 gcgaacgatc atgtcgagat cgaaatctcc ggcatgatcc ttgagccatt gccggcaatc
      661 gtcgctggcc tcgttgatga cgatcgtcag gcgacgaata ccggcggagc gaaaacgatc
      721 gagggcgtga tcgatcagcg gacgccctcc caccggaacc atcggcttgc tgacgcgata
      781 accgtccgcg cgcaggcggc ttccctcgcc tgcggcaatg atgccgccac ggacagtgga
      841 agatacggat gccggggtca ttgcaggaac ttcgtgagat cgcgcaagtc ggtcaccgcc
      901 tgctgtccac tttgctccgc ctggacgtcc tgcggcgcga cccagatgaa gcgcatgcca
      961 agccgccgcg caccctcgcg atcgcggcgc aatgaatcgc cgatgaacac agtctcctcc
     1021 ggcgttgccg ccagtctgtc gagtgccgcg cggaaaatcg ccggatcagg cttctcggcg
     1081 cccacgcagt ggctgtccac catcacctcg aaaaaggagc acaggccggc gctttcgcac
     1141 actgcttcga gattaccgta gaaattcgac acgatgccga gccgatagcg ttcgctcagc
     1201 gcctcgagca tcggccgatt gcgctcgaag gtagccacgg cttccgagag aaagcccgct
     1261 gcaatacgcc ggccgcggtc gccgcttccg ttgcccgcac ggcgcgcgag ttcgctttcc
     1321 aggttggcgg tcaatgcgtg aactgtcccc gagagatcca ttgtggacgg caggcctccg
     1381 acaagcggat catcggccgc atggaacgcc ggcgcaaaag cctctccatc aagatttagc
     1441 ccctgggcgc gataaagtgc gtgaaaacgc tccttccacg cgacgccgtc agcgtcgagc
     1501 gtgccgccga aatcgaacag cagcgttttg atcggcgcgt tcattgcatg gcccgtcgct
     1561 gagccgcttg acacacaaca cccgggatgg ccgcgagcac gaagtcccgt tcgcgcctgc
     1621 tatgagcgct gcgggctcga tgccagcttg gcgttcggct cctccccggg aaggtcaaac
     1681 cggtggccct cgatgaaggc gttgcatgcg tcgagcgcaa cactgtcgcg caactgtcgc
     1741 ggcgggcttt tcatgtaata cgccgaaggc gcctcgagcg ggccggcttc gccgcgttcc
     1801 agcgcgagct tcacgcagcg aatcgcgtcg atgacgacgc ccgcgctgtt cggcgaatcc
     1861 tgcaccgaca gccgaagttc cagctcgatc ggtgcgtttc caaagccgcg cccttccatc
     1921 cggatgaagc agatcttgtt gtcctgttgc caaggcacat agtccgacgg accgatgtgg
     1981 atgtcacgcg catccagacg ctcatccagc tgcgattgaa cggactctgt cttggatact
     2041 tttttggact tgagccggct tttttcgagc atgttgagga agtcggtatt gccgccggtg
     2101 ttgagctgat aagtgcggtc gaggccgacg ccgcgatcgg caaagagtcg cgccagcgcg
     2161 cggtgcacga tggtcgcgcc gacctggctc ttgatgtcgt cgccgatgat cggcaggccg
     2221 gcatcccgga atttccctcc ccagacttca tccgaagcga ggaacacagg aacgcaattg
     2281 accatggcga cgccggcatc aagacaggcc tgcgcgtaat gcttgaccgc gcgttccgag
     2341 ccgacgggca gatagcagac aagcacctcg gcacccgacg cgcgcagcgc tgcagcgaca
     2401 tcggtcggct cgagatcgac gggacggaaa gcgctatcgt ccgggtagtc ggccatgtgc
     2461 gccgcgacac cgtcgagaag cgggccggcc tgcacgatga cgttcgacac cggcaacgcg
     2521 ctttgaaaaa tgcgcgtgca attcggcttg gcgaaaatgg cctcttccag cggatgaccg
     2581 accttgcgcc ggtcgacgtc gaatgcagcg acaaattcga cgtcgctgca gcgatacccg
     2641 ccgatcaaat gacgcataac tccttcaagc tcagcaccct gacgatcccg gtaatattct
     2701 acgccctgca ccaatgaact ggcgcaattg ccgac
//
LOCUS       DASSRL010000013         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_122814, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000013 DASSRL010000000
VERSION     DASSRL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122814"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VFL49_00230"
     CDS             complement(<1..2056)
                     /locus_tag="VFL49_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7910434.1"
                     /translation="MVQIVILDDRETNRKIFSKLAGSIEPGVTVHSFGNPVELLGWLE
                     HNTPDLIVTDFKMPSMDGAEFICRLRALPHCGEVPVIVITVYEEREFRLRALEAGATD
                     FLNSPVDHQEFVTRARNLLKLRKQQIELAARAENLAQELKDSERSREEALRDSSERLA
                     QVIDTVPAMISATDSQGRVLFMNAYLASLAGIGPTEATGSDIRPVFGEDHAARSKALD
                     RKVVSTQAPLPSFEEEIIDCNGAKRVFLTTKTPLRDYSHAVVGVLTSSLDITDRKRAE
                     NHLFYMAHHDVLTNLANRALLSDRLRREIARTRRGDSPFALHLIDLDGFKDINDGLGH
                     QVGDQFLVEVANRLRSVVRDTDTVARLGGDEFAILQTRVTRNEDTVDMANRILEAINK
                     PWSHAGEQVSLSASIGITVHPTDGTDVQELLRNSDLAMYRAKHDGGKVFRFYASDMDR
                     RARASLILDTDLRAAVDKNQFILHYQPQIALRTGRVTGAEALLRWQRPGHGLIGPSEF
                     LARAEENGLIIPINEWALREACRQAKLWQQRGHPPTRVGVNLSSIQFFKQNVPLMVAK
                     VLAETGLDPWLLDLELTESIVMHNADAVSKDLQQLRELGVGISIDDFGTRYSTLAYVK
                     HFPVDRLKIDQCFVRDIATNPSDAAIVRAIVSLGHSLDLEIVAEGVETVQQSTMLRAE
                     GCD"
     gene            complement(2312..3322)
                     /locus_tag="VFL49_00235"
     CDS             complement(2312..3322)
                     /locus_tag="VFL49_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7910435.1"
                     /translation="MTVVILKEGYSSTAIAPIEIFHSAGVIWNWLHGEAVQPRFRVRA
                     ASVDGRKVKTVCSLGLDPECAIGDIKSSDIVIVPASGWDVLEQIAHNTPLLPWLKKQY
                     EQGACIAGVCSGVAFLAECGILDGRRATTHWALADILQKRYPKVRWQPEQFVTEDGAL
                     FCSGGVYAAIDLSLYLVEKFCGHEVALKVAKSLLLSMPRGRQSGYSVVQLSRPHSDEK
                     VRQTEEYLQQHFDSDVSIEHLAERAGMGPRNFIRRFKAATGRVPGAYLQTLRVSAAKE
                     LLETGTSSIQAICAKVGYEDIGFFRNLFKRHTGMTPGEYRDRFSQMSFNRGDLAGGRQ
                     SA"
     gene            complement(3339..>3590)
                     /locus_tag="VFL49_00240"
     CDS             complement(3339..>3590)
                     /locus_tag="VFL49_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910436.1"
                     /translation="MTSHWTPYVRVSNICHAAQRATALGGTVVVDPFVVTGIAQIALI
                     LDSVGAIVGLWEPITSDGKANDRGQEASIAYKTSAPLRR"
BASE COUNT          664 a         1069 c         1156 g          701 t
ORIGIN      
        1 cgtcgcatcc ctcggcacgc agcatggtcg actgctgcac cgtttcgacg ccttcggcga
       61 cgatctcaag gtccaggctg tgcccgaggc tcacgatcgc gcgcacgatc gcagcgtcgc
      121 tggggttggt cgcaatgtcg cgcacgaaac actggtcgat tttcagccgg tcgacgggga
      181 aatgcttgac gtaggcgagc gtcgaatagc gggtaccgaa gtcgtcgatc gagatgccga
      241 cgcccagctc gcgcagctgc tgaaggtcct tcgacacggc atcggcgttg tgcatcacga
      301 tgctttccgt gagctcgagg tcgagcagcc acggatcgag cccggtttcg gccaggacct
      361 tggccaccat cagcggcacg ttctgcttga agaattggat tgacgacagg ttgacgccga
      421 cgcgggtcgg cggatggccg cgctgctgcc agagcttcgc ctgccggcaa gcctcgcgca
      481 gtgcccattc gttgatcgga ataatcaggc cgttctcttc ggcccgggcc aggaattcgc
      541 tcggaccgat caggccgtgc cccggacgct gccagcgcaa cagggcttcc gcgccggtaa
      601 cccgtccggt ccgcagcgcg atctgcggct ggtagtgcag gatgaactgg ttcttgtcga
      661 ccgcggcgcg caggtcggtg tccaggataa ggctggcgcg ggcgcggcgg tccatgtccg
      721 aggcgtaaaa gcggaagacc ttgccgccat cgtgcttggc ccggtacatc gccaggtcgc
      781 tgttgcgcag aagttcctgc acgtcggtgc cgtcagtcgg gtgcaccgtg atgccaatgc
      841 tggcgctcag cgaaacctgc tcgccggcgt gggaccacgg cttattgatc gcctcgagaa
      901 tgcggttggc catgtcgacg gtgtcttcgt tgcgcgtgac acgcgtctgc aggatcgcga
      961 actcgtcgcc gccgagacgg gcgaccgtat cggtgtcgcg cacgaccgag cgcagccggt
     1021 tggcgacttc gacgaggaac tggtcgccga cctggtggcc cagtccgtca ttgatgtcct
     1081 tgaagccgtc cagatcgata agatggagcg caaatgggct gtcgccgcgg cgggtccgtg
     1141 caatctcgcg gcgaagccgg tcggacagca gcgcgcggtt ggcgaggttg gtcagcacgt
     1201 catggtgcgc catgtaaaag agatggtttt cggcgcgctt gcggtctgtg atgtcgagcg
     1261 aggacgtcag cacgccgacc acggcatggg agtagtcgcg caacggcgtt tttgtagtca
     1321 gaaaaacgcg cttggcgccg ttgcagtcga tgatctcttc ctcgaaactc ggtagcggcg
     1381 cctgcgtgct gacgactttg cgatcgagtg ccttgctgcg cgcggcatgg tcttcgccga
     1441 agaccgggcg tatgtcgctg ccggtcgcct cagtcgggcc gataccggcc agtgaggcta
     1501 gataggcatt catgaacagc acgcgaccct ggctgtcggt cgcgctgatc atggctggaa
     1561 cggtgtcgat aacctgcgcc agccgctcgc tcgaatcgcg caatgcttcc tcgcgcgagc
     1621 gctcggagtc cttgagttcc tgcgcaagat tttctgcgcg ggccgcgagc tcgatctgct
     1681 gcttgcgcag cttcaggaga ttgcgcgcgc gggtcacgaa ctcctgatgg tcgaccgggc
     1741 tgttaaggaa gtcggtcgcg ccggcctcga gcgcgcgcag ccggaattca cgctcctcgt
     1801 agaccgtaat gacgatgacc ggcacttcac cgcagtgcgg cagagcgcgc aggcggcaga
     1861 tgaattcggc gccgtccatc gacggcatct tgaaatcggt gacgatgagg tcaggcgtat
     1921 tatgttcaag ccagccaagg agttcgaccg gattgccgaa gctgtggacg gtcacgccgg
     1981 gctcgattga accggcgagc ttcgagaaga tcttccgatt ggtttcccgg tcgtcgagaa
     2041 ttacaatctg taccattgcg tcccaggtct gctgcggccc cgagcaaaga ctgctccacc
     2101 ggacagcgga cactacgcag tggctcatta agcgattgga gggacattac ctcaacttgt
     2161 aataaaaaac ttcctattaa cgctgggcgc gcgcgtagtt tgcgcagccg cgcgcgggta
     2221 ccgcgaaaaa gccgcgtcgt tttagaccgc gccgcgttaa aatcggcgtg aagttcaagg
     2281 caaagcccgc acgaccgtaa cgcccggcgc gtcaggcact ctgccttccg ccagccagat
     2341 cgccgcgatt gaagctcatt tgcgaaaacc ggtcgcggta ttcgccgggc gtcattccgg
     2401 tgtgacgttt gaacaggttg cggaagaagc cgatgtcctc gtaaccgacc ttggcgcaga
     2461 tggcctggat cgaggaagtg ccggtttcca gcagttcttt ggctgccgag acgcgcaggg
     2521 tttgcagata ggcgccaggt acgcggccgg tcgcagcctt gaagcggcgg atgaaattac
     2581 gcgggcccat gccggcgcgc tcggcgagat gctcgatcga gacgtcgctg tcgaagtgct
     2641 gttgcagata ttcctcggtc tggcggacct tctcatcgga atgagggcgc gagagctgca
     2701 ccaccgaata tcccgactgg cggccgcggg gcatgctcag cagcagcgat ttggcgactt
     2761 tcagagcgac ttcgtgaccg cagaatttct cgacaagata gagacttagg tcgatcgcgg
     2821 cataaacgcc gccgctgcag aacagtgcac cgtcctcggt cacgaactgt tccggctgcc
     2881 agcgcacctt tgggtagcgc ttctgcaata tgtcggcgag agcccaatgc gttgtcgcgc
     2941 gcctgccgtc gagaatgccg cactcggcaa gaaaagcgac accggaacac acgcctgcga
     3001 tgcaggcgcc ctgttcatac tgctttttca gccacggcag cagtggtgtg ttgtgggcga
     3061 tctgctccag cacatcccat ccggacgcgg gaacaatgac gatgtcgctg ctcttgatgt
     3121 ctccgatggc acattcggga tcaagcccaa gcgagcagac cgttttgacc ttgcggccgt
     3181 cgaccgaagc agcgcgtacg cggaatcgtg gttgcacggc ttcgccgtga agccagttcc
     3241 agattacgcc ggcagaatga aatatctcga tgggagcgat tgcagtcgac gagtagcctt
     3301 cctttagaat aacgacggtc acgtccagca accgatcgct atctgcggag aggggcgctc
     3361 gttttgtacg cgatgctggc ttcctggcca cggtcgtttg cctttccgtc tgacgtgatc
     3421 ggctcccata ggccgacaat tgcgccaacc gaatcaagga tgagcgcaat ttgcgcgatc
     3481 ccggtcacga cgaacggatc gacgaccacc gtaccgccga gcgcagtcgc ccgctgtgcg
     3541 gcatggcaga tgttgctcac acggacatag ggcgtccaat gcgaggtcat
//
LOCUS       DASSRL010000014         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1120129, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000014 DASSRL010000000
VERSION     DASSRL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120129"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFL49_00245"
     CDS             <1..577
                     /locus_tag="VFL49_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET7910437.1"
                     /translation="RALMKKHGFLDVLYSLGIANPGAMRIQNYPNFLRKFSKKGQPLL
                     DMAAVDIMRDRERGVPRYNRFRELVGKKRVTSFEEITSIPGIAGKMRKIYKDDVDRVD
                     LLVGLLSEDLPEGFGFSDTAFRVFALMASRRLKSDRFFTDDFRPEIYTDFGMDWIRNN
                     SMKSVLLRHFPRLGPALEGVNNGFAPWNVVA"
     gene            587..1480
                     /locus_tag="VFL49_00250"
     CDS             587..1480
                     /locus_tag="VFL49_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910438.1"
                     /translation="MAIRITIPGIVRLAWLRQPDEVIAANDSAVVQRSLSGRGGLWQR
                     SIAAKLAVFQTADGNIWPAFRDRLDPLRAKQQSELEAAMSDTSGLLARLAPETDALAA
                     YVRSGQTNRPPEIIVQQMVGRLFFPDYAASEESYDAAWTLQAWLASGPIKSYSLKCSG
                     ELERAIDRIISLAHGNTSCAHATAIAMENIVNAIERMRRLARRGNNLGKLSPEEVLAC
                     TLRAPKRVIRETRDGGRVGGTRFGARTLLILGLEAARRHNPEAGIGFFVHGWNRCPAH
                     AIVPALLAEVWKKAKARAETA"
     gene            1564..2589
                     /locus_tag="VFL49_00255"
     CDS             1564..2589
                     /locus_tag="VFL49_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7910439.1"
                     /translation="MRHHKFLTFLGAAALAVLSVVTPAHAQVLKAVKDRGVLNCGVSE
                     GLNGFSARDSNGNWSGFDVDTCRAIAAAVFNDAGKIKFVPLDANRRFEALRSGEIDVL
                     SRNTTWTISREVDLGLIFAATNYYDGQGFLIPRSMNKETTLDLNGVKVCVQTGTTTEL
                     NLADYFRVNNMKYEALASPTLEAAIKGYDSGQCNALTADVSALWGIRLLLSKPEEHVI
                     LPDVISKEPLGPAVRQGDMQWLNIVKWVSFALVNAEELGVTSKNIDEALKSEKPDIKR
                     LVGIEGGYGERMGLANDWAARVIRLVGNYGEIYERNVGVGSPLGIPRGINQLWNKGGI
                     LYAPPIR"
     gene            2678..>3247
                     /locus_tag="VFL49_00260"
     CDS             2678..>3247
                     /locus_tag="VFL49_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910440.1"
                     /translation="MVDYVQLISRAVATLNPNTREARQALYQRARQTLADKLRSIDPN
                     LSHTDLNAERAALESAIQQVERNTIRHAAPPQPRPVRERYDYRDRPPLKENRNWLRTA
                     AVALGVVLVFAAGAALYSFWPRSLDDVRSIAKERKERSASSISDTAEIKSDYVRLRQL
                     VYYRTTQPEGTIVVDKSQTFLYVVRPNLSA"
BASE COUNT          679 a          985 c          973 g          610 t
ORIGIN      
        1 gcgcgcgttg atgaaaaagc acggatttct cgatgtgctt tactcgctcg gcatcgcgaa
       61 ccccggtgca atgcgcatcc agaactaccc gaactttctg cgtaaattca gcaagaaggg
      121 acagccgctc ctggatatgg cggcggttga catcatgcgc gatcgcgagc gcggcgttcc
      181 gcgctacaac cggtttcgcg aactcgtggg aaagaagcgg gtgacgtcct tcgaggaaat
      241 caccagcatc ccgggcatcg ccggcaagat gcgcaaaatc tacaaggacg acgtcgatcg
      301 cgtcgatctg ctggtggggc ttctttccga agacctgccc gaaggcttcg gcttcagcga
      361 taccgctttc cgtgtctttg ctctgatggc atcacggcgc ctgaagagcg accgtttctt
      421 tacggacgac ttccggccgg aaatctacac cgacttcggc atggactgga ttcgcaacaa
      481 ctcgatgaag tcggtacttc tccgtcactt cccgaggctc gggccggcgc tcgaaggcgt
      541 gaacaacggc ttcgcaccct ggaatgttgt ggcttgagtg actggcatgg caatcaggat
      601 cacaattccc ggcattgttc ggctcgcctg gttgaggcag ccggacgaag tgatcgcggc
      661 caatgacagc gccgtcgtgc agcgctcgct ctccgggcgc ggagggctgt ggcaacgctc
      721 catcgcggca aagctggcgg tcttccagac ggctgacgga aatatctggc cggcctttcg
      781 cgaccggctg gaccccctgc gcgcgaaaca gcagagcgag ctcgaagccg cgatgtccga
      841 tacatcgggc ctgctcgcgc gccttgcgcc ggaaacggat gcgcttgcgg cttacgtccg
      901 cagcggccag acgaaccgcc cacccgaaat tatcgtccag cagatggtcg gccggctttt
      961 ctttcccgac tatgcggcaa gcgaggaatc ctacgacgct gcgtggacgc tgcaggcctg
     1021 gctggcgagc ggaccgataa agtcatattc gctgaagtgc agcggtgaac tcgagcgcgc
     1081 gatcgaccgg attattagcc ttgcacatgg caacacatca tgcgcgcacg ccaccgcaat
     1141 cgcgatggag aacatcgtca acgccatcga gcggatgcgc cggctcgcac gcaggggaaa
     1201 caatctcgga aaactcagcc cggaggaagt gctggcatgc acattgcgcg cgccgaagcg
     1261 cgtcatccgc gagacgcgcg acggcggccg cgtcggcggc actcgcttcg gcgcgcgcac
     1321 gctcctcatt cttggccttg aggccgcacg gcggcacaat ccggaagccg gcatcggctt
     1381 ctttgtccac ggctggaacc gctgccctgc ccacgccatc gtgcccgcgc tgctcgccga
     1441 ggtctggaaa aaagcaaaag cacgcgcaga gaccgcttag tcggttgcgc ggctgtgacg
     1501 ggcaagccct tccattggca agccaaatcg ctacgatgcc ctctcgtgaa attcggggcc
     1561 gtaatgcgac atcacaaatt tctgacgttt ctaggcgccg cagcactggc ggtgctttcc
     1621 gttgtcacgc cggcgcatgc gcaggttctc aaggccgtta aggatcgcgg cgtgcttaat
     1681 tgcggcgtga gcgaaggcct gaacgggttt tccgcccgag acagcaacgg caactggagc
     1741 ggcttcgacg tcgatacgtg ccgggcgatc gccgcggcag tcttcaacga tgccggcaag
     1801 atcaaattcg ttcctctgga cgccaaccgg cgatttgaag ctctccggtc cggcgagatc
     1861 gatgtccttt cgcgcaacac cacctggacg atctcgcggg aagtcgacct cgggctgatc
     1921 tttgcagcga cgaactacta cgacggacaa ggattcctta ttcccagatc catgaacaag
     1981 gagacgacgc tcgatctcaa cggcgtgaaa gtctgcgtgc agaccggaac gacgaccgag
     2041 ctcaaccttg ccgactattt ccgcgtcaac aacatgaagt acgaggcgct cgcctcgccg
     2101 acacttgagg cggcgatcaa aggatatgat tccggtcaat gcaatgcgct cacggcagac
     2161 gtatcggcgc tgtgggggat tcgacttctg ttgtcgaagc cggaagagca cgtcattctg
     2221 ccggatgtca tttccaagga gccgcttggc cctgccgtgc gccaagggga catgcaatgg
     2281 ctgaacatcg tgaaatgggt gagcttcgca ctggtcaacg ccgaggaact cggcgtgacc
     2341 tccaaaaata ttgacgaggc tctcaagtct gaaaaaccgg atatcaaacg actcgtcgga
     2401 atcgaaggcg gctacggtga gcggatgggc ctggccaatg attgggccgc ccgcgtcata
     2461 agacttgtgg gaaactatgg agaaatatac gagcgaaacg tcggcgtcgg ctcgccgctc
     2521 ggcattccac gcggcatcaa ccagctgtgg aacaaaggcg gtatccttta cgctccgccg
     2581 atccgatgat cgtgatcgcg ggtaacaagt caggcataat gcgctcgtca atgatgaatg
     2641 gtaacgtggt tgcgtttctg ggcgagttcg aggtccgatg gtcgactacg ttcaactgat
     2701 cagtcgcgcg gttgcgacac ttaatccgaa tacccgggaa gcgcgtcagg ctctctatca
     2761 gcgggcccgg caaacactgg ccgataagct ccgcagcatc gacccgaatt tgtcccacac
     2821 cgacctgaat gcggaacggg ccgcgctcga atcagcgatc cagcaggtcg aacgaaacac
     2881 aatacgccac gccgcgccgc ctcaaccgcg gccggtccgc gaacgctacg attatcgcga
     2941 cagaccacct ttgaaggaaa acaggaactg gctgcgtacc gctgctgtcg cgcttggggt
     3001 ggtgctcgtt ttcgccgctg gcgcggccct ctattcgttc tggccgcgca gcctcgatga
     3061 tgtacgcagc atcgcgaagg agcggaagga gcggagcgcc tcctccattt cggacacggc
     3121 ggagatcaag tcagactacg tacggttgcg ccagctggtt tactaccgca ccactcagcc
     3181 tgagggaacg atcgtggtcg acaaatcgca gacctttctc tacgtcgtgc gacccaatct
     3241 ctcggcc
//
LOCUS       DASSRL010000015         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1277651, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000015 DASSRL010000000
VERSION     DASSRL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277651"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /locus_tag="VFL49_00265"
     CDS             <1..1732
                     /locus_tag="VFL49_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910441.1"
                     /translation="NNKPASASYSNGMTATWTYNADGSLHEIVYNGIVGARYTASETL
                     YANGKPVSAVWTNGSTVMQTETWNADGTVHDIHYYGLTGAYADYEVIYANNKPASATY
                     SNGMTQAWNYNADGSLHEVVYNGIAGARYTSSETLYANGKPVSAVWTNGATVMQTQAW
                     NADGTVHDIHYYGLTGAYTDYDVLYANNKPASATYSNGMTASWSYNADGSLHEVVYDG
                     IAGARYTSSETLYANGKPVSAVWTNGETVMQTQTWNADGTVHDVHYYGLTGAYSDYDV
                     VYANNKPASASYSNGMTQSWGYNADGSLHEVVYNGIVGQPWTSSETFYENGKPVSAVW
                     TTGDATSQSEAATIVRSQTWNADGSVHDIHYFGITGQAYTDYDVIYANNKPVSALYSN
                     GMTQTWTYNSDGSSVISFDNVEGKSYTATASIYDPHNDVSGHLALQQVTNTNGTQTLR
                     SYDDGLTVTIGSAGANVQLPGASGNAFHFDFKPETAVTGSTGADNLVLQSGFGHVTVT
                     AFGLESGAGTNNDVVTISGGAFKDFADMLAHASQDAAGNTHITDAAGDVLTLSHVSIA
                     ALQPNHFIIT"
     gene            1781..2008
                     /locus_tag="VFL49_00270"
     CDS             1781..2008
                     /locus_tag="VFL49_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910442.1"
                     /translation="MSDWMAIAQWQECQKLARPGIVFELRNAEGLTMLSPCVVQVPAK
                     PFDWKSPPLVFRAVAAPKPQHSTPMPEPRGR"
     gene            complement(2382..3230)
                     /locus_tag="VFL49_00275"
     CDS             complement(2382..3230)
                     /locus_tag="VFL49_00275"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET7910443.1"
                     /translation="MPPIGVAFDMGPAAAPGAVAPVQDAALNLAHLESKRIIAHNVAD
                     LRSRSFDMLRTQVLQSMDLKSWQFLGVTSATEGCGKSVVAINLAMSIARQPERSVLLV
                     DLDLQKPQVASYLGLSCDRGVVSALQGRTSLQGSVIRARARNEELLVLPCAAPTINSS
                     ELIASRAMRGLMTDIKRDFRACTVIFDLPPILSSDDVISLLPQIDCVLFVVGAGTTTA
                     EEIKECNRHLDSAEVVRVVLNKAEDADPGHYSYPQPVQGPAPGKKPGSDRVKPVSKLL
                     KKLGEI"
     gene            complement(3317..4678)
                     /locus_tag="VFL49_00280"
     CDS             complement(3317..4678)
                     /locus_tag="VFL49_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HET7910444.1"
                     /translation="MLQRQTYHDPQLDAEAGLSLTPDYLLGVLRRRALYFALPCALVF
                     VIGSAIAWLWPARYISQGTILVQSQEIPSDLVRPTIAALANDRIQIIEQRVMTRDNLL
                     GIAKKYGLTSGWLERLSGTEIVDFLRKRTLIKPSEQKLESRKKEAIAFTIGFEYENPA
                     IATKVANELVTMMLSEDVRSRTEFAAQTTRFLAQEVTRLETRLASLDAQIIALRKTRL
                     GTLGSKFQLDGEKSLETLKAELMVKSATYSANHPDIQALKRKIAALEKGGVSDTTTAG
                     IGTDGTAGSTRAAGSAGLDTLETQRDTVKEELNKATQKLSAARMGENLEKGQHSERLE
                     VIEQPTMPYKPVSPNRPKIFIMVMALSLMAGGGMVFAAEMLNQSIRTRADLYSLIDRQ
                     MIVSIPYIATHDEVTSRRTKRLRWIVLGALAVIAGLTVLYFVLPPLDILLDNVMRLVT
                     RRF"
BASE COUNT         1023 a         1767 c         1740 g          989 t
ORIGIN      
        1 caacaacaag ccggcgagcg cgagctactc caacggcatg accgcgacct ggacgtacaa
       61 tgccgacggc tcgctgcatg agatcgtcta taacggcatt gtcggcgcgc gttacacggc
      121 gagcgaaacg ctctacgcca acggcaagcc ggtgagcgcg gtgtggacga acggcagcac
      181 ggtcatgcag accgagacct ggaatgccga cggcaccgtg cacgacattc actactacgg
      241 gctgaccggg gcgtatgccg actacgaggt catttacgcc aacaacaagc cggcgagtgc
      301 gacttactcg aacggcatga cgcaggcctg gaactacaac gccgacggtt cgctgcacga
      361 ggtcgtctat aacggcattg ccggcgcgcg ttacacttcg agcgagacgc tctacgccaa
      421 cggcaagccc gtcagcgccg tgtggaccaa tggcgccacg gtcatgcaga cgcaggcctg
      481 gaatgccgac ggcaccgtgc acgacattca ctactacggc ctgaccggcg cctataccga
      541 ttacgacgtg ctctatgcca acaacaagcc ggccagcgcg acctactcca acggcatgac
      601 cgctagctgg agctataacg ccgacggctc gctgcacgag gtcgtgtatg acggcattgc
      661 tggcgcgcgt tacacttcga gcgagaccct ctacgccaac ggcaagccgg tgagcgcggt
      721 gtggaccaac ggcgaaacgg tcatgcagac ccagacctgg aatgccgacg gcaccgtgca
      781 cgacgttcac tattacggcc tgaccggtgc ctacagcgac tacgacgtgg tctatgccaa
      841 taacaagccg gccagcgcca gctattcgaa cggcatgacg cagagctggg gctacaacgc
      901 cgacggctcg ctgcacgagg tcgtctacaa cggcatcgtc gggcagccct ggacgtcaag
      961 cgagacgttc tacgagaacg gcaagccggt gagcgcggtg tggaccaccg gcgacgcaac
     1021 cagccagagc gaggcggcaa cgatcgtgcg cagccagacc tggaatgcgg acggctcggt
     1081 gcacgacatc cattatttcg gcatcaccgg ccaggcctat accgactacg atgtcatcta
     1141 tgccaacaac aagccggtca gcgcccttta ctccaacggc atgacgcaga cctggaccta
     1201 taacagcgac ggctcgtcgg ttatcagctt cgacaacgtc gagggaaaaa gctacaccgc
     1261 gaccgccagc atctatgacc cgcacaatga cgtcagcggt catctcgcct tgcagcaggt
     1321 gaccaatacc aacggcaccc agacgctgcg cagctatgat gacgggctga cggtcacgat
     1381 cggatcggcg ggcgcaaacg ttcagttgcc aggcgcgagc ggcaacgcct tccattttga
     1441 cttcaagccg gagacggccg tcaccggcag cacgggcgcc gacaatctcg ttcttcagtc
     1501 aggctttggc cacgtcaccg tcaccgcctt tggcctcgag tcaggggcgg gcacgaacaa
     1561 cgatgtcgtc accatcagcg gcggcgcctt caaggatttt gccgacatgc tggcgcacgc
     1621 atcccaggat gccgcgggga atacccacat caccgatgcg gccggcgacg tgctgacgct
     1681 cagccatgtc tcgatcgccg cgcttcagcc caaccatttt atcatcacgt aggtacgacc
     1741 cgtggccgcg gcggtaccgt cggaggaggg gaggatcccg atgagcgact ggatggcgat
     1801 cgcccaatgg caggagtgcc agaaactggc gcggcccggc attgtgttcg agctcaggaa
     1861 tgcggaaggc ctgacgatgc tgtcgccctg cgttgtacag gtgccggcaa aaccgttcga
     1921 ctggaaatcg ccgccgctgg tcttccgcgc ggtcgctgcg ccgaagccgc agcactcgac
     1981 gccgatgccg gagccgaggg gcaggtgacg cgccttggcg cgccttggcg cgccttggca
     2041 cgtcatggcc ggacttgttg ttcagccatc cacgtctttc cggtctgtga gggcgtaaag
     2101 acgtggatac gcgggtcaag cccgcgcatg acgctgtgct tgtggcaccg ccctcctcgt
     2161 gatggccgga cttgcccgcc ttcgcgctcc gcgcttccgc ctacgcgctg acgcgcttcg
     2221 gcggactcag gcccgccgta gctcgcgcag cgagcgtaga cgggttccgg ccatccacgc
     2281 ctgccttgct tcaagacgtg gatggccggg tcgagcccgg ccataacaac cggccatgac
     2341 aaattgagcg acagtgcgtg gatagagcga gagcggcggg gtcagatctc gcccagcttc
     2401 ttgagcagct tggagaccgg cttcacgcgg tcgctgcccg gcttcttgcc tggcgccggt
     2461 ccctgcaccg gctgcggata agaataatgg ccgggatcgg catcctccgc cttgttgagc
     2521 acgacccgca ccacctcggc cgaatcaaga tgccggttgc attccttgat ttcctcggcg
     2581 gtggtcgtgc cggcgcccac gacaaacagc acgcagtcga tctgcggcag cagcgagatg
     2641 acgtcgtcgc tcgacaggat gggcggcagg tcgaagatca ccgtgcaggc gcggaaatcg
     2701 cgcttgatgt cggtcatcag gccgcgcatg gcgcgcgagg cgatcagctc ggaggagttg
     2761 atggtcggcg cggcgcacgg cagcaccagc agctcctcgt tgcgcgcgcg cgcccggatg
     2821 accgagcctt gcagggatgt gcggccctgc agcgcgctga ccacgccgcg gtcgcagctc
     2881 agccccagat aagaggccac ctgcggcttc tgcaggtcga ggtcgaccag cagcaccgag
     2941 cgttccggtt gccgcgcaat gctcatcgcc aggttgatgg cgaccaccga tttgccgcag
     3001 ccctcggtcg ccgaggtgac gccgaggaat tgccaggact tcagatccat cgactgcagg
     3061 acctgcgtcc gcagcatgtc gaacgaccgc gaccgcaggt cagcgacatt gtgcgcgatg
     3121 atccgtttgc tctcgagatg cgcgaggttg agcgcggcgt cctgtaccgg tgcaacggcg
     3181 ccgggtgcgg ccgccggtcc catgtcgaat gcaacgccga tggggggcac aggcgagggc
     3241 ccgtctcccc ttagcggggt ccttgcccgc tccaccgctt tcctgatgtt gtccatcact
     3301 tgctcaatct gcgaactcag aaccggcggg tcaccagccg catgacattg tcgagcagaa
     3361 tgtcgagcgg cggcagcacg aagtacagga cggtgaggcc ggcgatcacc gccagcgcgc
     3421 cgagcacaat ccatcgcagt ctttttgtcc tgcggctggt aacttcgtca tgggtcgcga
     3481 tatagggaat cgacacgatc atctgccggt cgatcagcga atagaggtcg gcgcgcgtgc
     3541 ggatcgactg gttgagcatt tcggcggcaa acaccatgcc gccgccggcc atcagcgaca
     3601 gcgccatcac catgatgaag atcttcggcc ggttcgggct gaccggcttg tacggcatgg
     3661 tcggctgctc gatcacctcc agccgctccg agtgctggcc tttttccagg ttctcaccca
     3721 tcctggccgc cgacaatttc tgcgtcgcct tgttgagctc ctccttgacg gtgtcgcgtt
     3781 gcgtctcgag cgtgtcgagg cccgcgctgc cggcggcgcg ggtcgagccg gccgtcccgt
     3841 cggtgccgat gccggcggtc gtggtgtcgc tgacgccgcc tttttccagc gccgcgattt
     3901 tccgcttcag cgcctggatg tccggatgat tggccgaata ggtcgcgctc ttgaccatca
     3961 gctcggcctt cagcgtctcg agactctttt cgccgtcgag ctggaatttg ctgccgagcg
     4021 tgccgaggcg ggttttgcgc aaagcgatga tctgcgcgtc gagcgaggcg aggcgcgttt
     4081 ccagccgtgt cacctcctgg gcgagaaagc gcgtcgtctg cgcggcgaac tcggtgcgcg
     4141 agcgcacgtc ctcgctcagc atcatggtga ccagctcgtt cgccaccttg gtcgcgatcg
     4201 ccgggttctc gtattcgaag ccgatcgtga aggcgatcgc ctctttcttt ctggattcga
     4261 gtttctgttc cgacggcttg atcagcgtgc gcttgcgcag gaagtcgacg atctccgttc
     4321 ccgataaacg ctcgagccag cccgaggtca ggccgtattt cttcgcgatg ccgagcagat
     4381 tgtcgcgcgt catcacgcgc tgctcgatga tctggatgcg gtcattggcg agcgcggcga
     4441 tggtcgggcg cacgaggtcg gacggaattt cctgcgactg caccaggatc gtgccctgcg
     4501 agatgtagcg cgccggccag agccaggcga tcgccgaccc gatcacgaac accagcgcgc
     4561 aggggagggc gaaataaagc gcgcgccgcc gcagcacgcc gagcaggtaa tcgggtgtca
     4621 gcgacagccc ggcctcggcg tccagttgcg gatcgtgata ggtttgccgt tgcagcatga
     4681 ctacttgact acttgcaaga cagcgaagcc cgcgcggaca gcccgaatga ccgcaatccg
     4741 ccggcgcagc tcactgcgtc tccagactca ggaccgttaa cgtcaccccg cgcatcaggt
     4801 tgatctacta ccgtagatta ccggtgggag taaggccaag ttttgcccgt gacggaatga
     4861 aagttaacct gcaaggccgg atttgttccg gcaggtcttc caaagccgct tgccgcaaga
     4921 cgtggatgcc cggcctaaag ccgggcatga cgacgtattt gtggcgcgtc ctatcagccc
     4981 gtcatggccg gacttggtcc ggccatctac gtctttcttg gagggcgtga agacaactcc
     5041 agcgtcatgc ccggccagcc gggtccggtc ttcggccggg ccgggcatcc acgccttcca
     5101 aagaacaaaa aggccgaatt tgttgccctt tttcaccaat ccccgtggaa aagcgccccc
     5161 aacaccgggt attcgacaca agaacgccca atggcggtac aatcatgggt attgcagccg
     5221 ggcaacgggc ccgcgctttt gcgcgctttg gggtaaccta aaaccgcaat tgcattggct
     5281 aacgccctgc agttcgggca catctgtatg ggggtgccgt tgggtcaggc gggcacggcc
     5341 ggcagcgcag gcgccgcggg ccacggggcc aaagggcctc gtgccggctg catgctgtgc
     5401 gggtttggga gtgctgatgt acgaggcgtt ttacgggctg cgtgaaaagc cgttctcgat
     5461 tctgcccgac cccgatctga tctattgggg ccgcgcgcac cgcatggcct tttccatgc
//
LOCUS       DASSRL010000016         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_647892, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000016 DASSRL010000000
VERSION     DASSRL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647892"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..91
                     /locus_tag="VFL49_00285"
     tRNA            15..91
                     /locus_tag="VFL49_00285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Arg,seq:acg)
     gene            complement(193..1452)
                     /locus_tag="VFL49_00290"
     CDS             complement(193..1452)
                     /locus_tag="VFL49_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910445.1"
                     /translation="MSSTEKAVSTSDALVAATKDKSVHHIVVHGNLTGAPTINLLPGQ
                     SLRGDGDAAAVSFAEGSDGLRLSSDNRIHNIRLNVAVEKRAIFNDTSVDSFGRIELRG
                     VTTTGRVQILARDKVRGGHVDVIGLDIVAADARAEKDRPQGYGVYVLQGAFTLWNMQQ
                     DTDVTISADLVGLSAGRDGAPVRGSGIFVSGGGDKAGKLAVRRLETDAVYSDGGIAAG
                     TPDQITGGVFTVYGAHVDVVRNRGPVTTYGVNDMVLDNWGVVDRWTAEAKITSHGPSG
                     IGFVNFGIVHELKVNAPIETFGQGARGFNVYTGTVNLAEFDRVTTHADGSVGIQISQP
                     IGRLVVHRGIETFGGTGPSLVKGKVVTLSAIGLSIKPGGSAREIEISGGIKTNGAGVS
                     PIEQHGAIEKLSVSGGFVAAGGGFDKI"
     gene            complement(1895..>2516)
                     /locus_tag="VFL49_00295"
     CDS             complement(1895..>2516)
                     /locus_tag="VFL49_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7910446.1"
                     /translation="EIVGAAGSVVGLDANPQMLAVARRKPARIEWLEGKAEALPLTDT
                     SFDAVVSQFGFMFFEDKPQALREMIRVLRPGGRLAVAVCDAVENSPGYNAFALLLDRL
                     FGTKVGDTFRGPFNLGDAGRLCEICRDAGIEDPEVVQRNGKVQFESINALVSTERACV
                     WTLGGVLTDEQFERLLKESETALKPFVTGGIVEFDMPSLIIRARKC"
BASE COUNT          474 a          819 c          752 g          471 t
ORIGIN      
        1 gtccgccttc cgaagcgccc gtagctcaac tggatagagc accagactac ggatctgggg
       61 gttaggggtt cgaatcctct cgggcgcgcc aaatttccac cactccagaa caaaactggg
      121 caccaagttt acattgatcg agacgggctt tcgaagaccg gatgctcagt tgtgcagcgc
      181 ctcagtcgcg tttcagattt tgtcgaagcc gccgcccgcc gcgacaaagc ctccgctgac
      241 gctcagcttc tcgatcgcgc cgtgctgctc gatcggcgac acgccggccc cattggtctt
      301 gatgccgcct gaaatctcga tctcgcgcgc cgagccgccg ggcttgatgc tgaggccgat
      361 ggccgaaagc gtgaccacct tgcccttgac cagcgacgga ccggtgccgc caaacgtctc
      421 gatgccgcga tggacgacga gcctgccgat tggctggctg atctggatgc cgaccgagcc
      481 gtcggcatgg gtggtgacgc ggtcgaattc ggcaagattg accgtgccgg tgtagacgtt
      541 gaagccgcgc gcgccctgac cgaaggtttc gattggcgca ttgaccttca gttcatgcac
      601 gataccaaaa ttgacgaaac cgatgccgct tgggccatgc gaggtgatct tcgcctcggc
      661 cgtccagcgg tcgacgacgc cccaattgtc gagcaccatg tcgttgacgc cgtaggtcgt
      721 caccgggccg cggttgcgca ccacgtcgac atgggcgcca tagacggtga agacgccgcc
      781 ggtgatctgg tccggcgtgc cggccgcaat gccgccatcg ctgtacacgg cgtcggtttc
      841 gagccgccgc accgcgagtt tgccggcctt gtcgccgccg ccactgacga agatgccgct
      901 gccgcgcacc ggggctccgt cacggccggc cgaaaggccg acgagatcgg cactgatggt
      961 cacgtcggta tcctgctgca tgttccacag cgtgaacgcg ccttgcagca catagacgcc
     1021 atagccctgc ggcctgtcct tttctgcgcg cgcatccgcc gcaacgatat cgaggccgat
     1081 cacgtcgaca tgaccgccac gcaccttgtc gcgcgccagt atctgcacgc ggccagtggt
     1141 ggtgacgccg cgcaactcga tccgcccaaa actatccacg gaggtgtcgt tgaagatcgc
     1201 acgcttttcc acggcgacgt tgaggcggat attgtgaatg cggttgtcgg acgagagccg
     1261 aagcccgtcg ctaccctcgg cgaaactgac ggcggcggca tcgccgtcgc cgcgcaaaga
     1321 ctgtccaggc aaaagattga tggtgggcgc gccggtcaaa ttgccgtgca ccacgatgtg
     1381 atgaacactt ttgtccttgg ttgccgcaac cagggcatcg gatgtcgaaa ccgctttttc
     1441 cgtactactc atgaaacgtc tccggcgaaa ggtttcgcac accacacagt tgcgcctgat
     1501 tgtgcgctct ctcgccccgg ggcatgcaaa cttttttcgt tgcaccgcat tatcgcgaag
     1561 acgctggagg ccgcagtgta gaacagcccg cattccagac acgacctcgg aaattaaatt
     1621 gcacaaacaa tgacatacga gtgcccagtt tcatcctctg tggcgcgcca aactctgccg
     1681 aatctgcgat ttcctccgac cggctacgca aggctgcgcc ggaagaaact cgttgtcgca
     1741 ggatgggcgg cattcggtga cattggcgtt cgcatcaatg gcactgctct ccagcgacca
     1801 aagaggcgat ggcaaagcat cgggagcgga cgggacggcc gggtgccgat gtccctcgtc
     1861 actagccgaa ctcgataccc tcggcgtcac cgcgttagca tttccgcgct cttatgatga
     1921 gcgacggcat gtcgaactcg acgatcccgc cggtcacgaa cggcttgagc gccgtctccg
     1981 actctttcag aagtcgctcg aattgttcgt cggtgagcac gccgcccagc gtccacacgc
     2041 acgcgcgttc ggtggatacc agcgcattga tcgactcgaa ctggaccttt ccgttgcgtt
     2101 gcacgacctc gggatcctcg atacccgcat cgcggcagat ttcacacagc cgtccggcgt
     2161 cgccgaggtt gaaggggcca cggaaggtgt cgcccacctt tgtgccgaag agtcggtcga
     2221 gcaacagcgc aaaggcgttg tagcccggcg aattctcgac cgcatcgcat acggcgactg
     2281 ccagccgacc gcccggtcgc aggacccgaa tcatctcacg aagcgcctgc ggcttgtcct
     2341 cgaagaacat gaagccgaac tggctgacga cggcgtcgaa gctggtgtcc gtgagcggca
     2401 gggcctcggc cttcccttcc agccactcga tccgagccgg cttgcggcgc gcgaccgcaa
     2461 gcatctgcgg attggcgtcc agcccgacga ccgagcctgc cgcgccgacg atctcg
//
LOCUS       DASSRL010000017         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1645150, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000017 DASSRL010000000
VERSION     DASSRL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645150"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1689)
                     /locus_tag="VFL49_00300"
     CDS             complement(244..1689)
                     /locus_tag="VFL49_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HET7910447.1"
                     /translation="MGLFGILLALALLMWLAYRGWSILLVAPLAALVAAAVSREPLLA
                     HWTQTFMGGAARFFIQWFPMFLLGGLFGKLMDDSGSISSIARFLTERLGTRNTMLAVV
                     LASALVTYGGVSVFVAFFVLVPMAQTMFKSADLPRRLLPATIGLGAFTFTMSALPGSP
                     SVNNAIPMPYFGTTTFAAPGIGIVASVITLAFGLWWLRRAEAAARKAGEGYGGDVDTA
                     PVIDEKTREHATAAVEFDPAEAHHGKHASSEPSFVLAVLPLFVVIVVNFLMSLIVFPR
                     LDFAFLAKEVWGGTTIGAVAGLWSLLLALGAGTLTIVIVNYSRLPSLRESLDAGANSS
                     ALPILTISSLVGFGAVVAALPAFSTVRDAVLSIPGGPLVSLTVSMNVLAGLTGTASGG
                     MAIALNALGDTFLRLGAEHGVDPALMHRITTISAGTLDALPHNGTVLMLLQISKLTHK
                     ESYGDMVMTVIVSVIISLVAILVLGSVFGSF"
     gene            complement(1703..2704)
                     /gene="yut"
                     /locus_tag="VFL49_00305"
     CDS             complement(1703..2704)
                     /gene="yut"
                     /locus_tag="VFL49_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HET7910448.1"
                     /translation="MKELLAGWEKLCASSAGLRFVDINLRGTGQVMLQDNPLSGALFL
                     AAVIWGAYAAGAPQIAVGGIVALLAATLTAYWVRADEKSLAEGLYGFNGILVGLALPT
                     FLAPGPLLWAYVILGGAVSTIVMIWTVKLVTPWGAALTFPFVIATWLLLLATYGFAGL
                     TGTALPAGKVVGAFQAVASDPLRLGDFVQGTLHSISQVFLKGNGLSALLLLAGLAVNS
                     LAAAAFGLGGAILAVIVAHLLGAESDLITGGLLGFSPVLTAIALGTVFYRPSLRVALY
                     TALGTAMTVVAQAALNVALTPFAIPALTAPFVLVTWMFMAPREHFERSTNRRSRKAA"
     gene            complement(2766..>3099)
                     /locus_tag="VFL49_00310"
     CDS             complement(2766..>3099)
                     /locus_tag="VFL49_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET7910449.1"
                     /translation="RLLFTEKLIIEPQRYSMRQTGVMDSFDVFGNVILCTPKDKAEHI
                     YARVENDIDLSRGVACGACRLPNDAGLIFKVVGRECAQVKTKIREFWGLVRKEVTGAD
                     LPQPFLWR"
BASE COUNT          765 a          927 c          885 g          522 t
ORIGIN      
        1 gcctgcacat ggaaatgaag aggatcatgg ccgatccgga aatgatcaag cttaccgaaa
       61 agatcggtct gcttccggtc gattcgcccg acatcgacgg catccagaga tatctggcct
      121 ccgagcgcga gaaatggggc accctggtga agcaactcgg cttggctggg acgatgtaac
      181 cagcccgccg ggcgggtata cgtttggacc aaattcataa ggcgttggca gttgtgcctt
      241 ccgtcaaaac gacccaaaca cggaccctag tacaaggatc gccacaagcg agataatcac
      301 gctgacgatc acggtcatca ccatgtcacc gtagctttcc ttgtgagtca gtttgctgat
      361 ttgaagcaac atcaggaccg taccattgtg aggaagagcg tcgagcgtgc cggcgctgat
      421 cgtggttatg cgatgcatca aagcgggatc gacgccatgt tcagcaccga gacgcagaaa
      481 tgtgtcgccg agtgcattga gtgcaatcgc catgccgccg gaagccgtac cggtcagccc
      541 ggcgagaacg ttcattgaga cggtgagcga gaccagtggc ccccccggaa tcgatagtac
      601 cgcgtcgcgt accgtagaaa acgccggcag ggcagcaacg acggcgccga aaccgacaag
      661 gctggagatc gtcaggatcg gcaaagccga agagttggcg ccagcatcca ggctttcgcg
      721 caaagagggc agacgactgt agttgacgat gacgatggtc agggtcccgg ccccaagtgc
      781 caggagcagc gaccacagac ccgccaccgc gccgatcgtg gttccccccc acacttcctt
      841 tgccagaaat gcaaaatcga ggcgtggaaa cacgatcaac gacatcagga aatttacgac
      901 gatgacaaca aaaagcggca gtacagccaa aacgaaggat ggctcgctgc tggcgtgttt
      961 gccgtggtgt gcctcggcag gatcgaattc gactgcagcc gtcgcatgct cgcgggtctt
     1021 ctcgtcaatg acaggagcag tgtccacatc gccgccgtag ccctcgccag ccttgcgcgc
     1081 ggccgcttcg gcacggcgga gccaccatag tccaaacgcc aaagtgatga ccgacgcgac
     1141 gatcccgata ccgggagccg caaatgttgt tgttccaaag tacggcatcg gaatggcgtt
     1201 attgaccgac ggtgagcccg gaagcgcaga catcgtgaac gtaaatgcgc cgagaccgat
     1261 cgtggcgggc aataaccgcc gcggaagatc ggcggactta aacatcgttt gcgccatcgg
     1321 taccaacacg aaaaaggcaa cgaagacgct gaccccgcca taggtgacga gggctgaggc
     1381 aagcacgact gcgagcatcg tgttgcgagt tcccaatcgt tcggtcaaaa aacgtgcgat
     1441 cgagctgatc gatccgctgt catccatgag cttgccgaac aaaccgccga gcaggaacat
     1501 cggaaaccac tggataaaga agcgggcggc accacccata aaagtctgcg tccaatgggc
     1561 gagaagcggc tcgcgcgata ctgcagcagc cacaagcgca gccaaggggg caaccagcaa
     1621 aatactccag cctcggtagg ccagccacat cagcaaggcc agcgcgagca atatcccgaa
     1681 aagtcccatg actcaggcct ccctacgccg ccttgcggct tcgtcgattg gtgctccgtt
     1741 cgaaatgctc acgcggcgcc ataaacatcc aggtcaccag tacaaatggt gcggtcagtg
     1801 caggaatggc gaacggcgtt aacgcgacgt tcagcgccgc ttgcgcgacg accgtcatcg
     1861 ccgtacccag tgcagtatac aacgcgacac gaagactcgg ccggtagaag acggtgccga
     1921 gcgcgatggc ggtcagtacc ggactgaagc ccagcagccc gccggtgatc aaatcgcttt
     1981 ctgcgccgag aagatgcgcg acaattacgg ccagtattgc gcccccaagc ccaaatgcgg
     2041 cggcggcaag cgaattaacc gccaatccgg caagcaacag cagcgcagac aagccgttcc
     2101 ctttcagaaa cacctgcgaa atgctgtgca atgtaccctg cacaaaatca ccaagccgaa
     2161 gcggatccga ggcaaccgcc tgaaaggccc cgacaacctt gccggccggt agtgcggtgc
     2221 ctgtcagccc cgcaaatcca taggtcgcca gaagaagaag ccaggtcgcg atcacaaatg
     2281 gaaaggtcaa agcggcgccc catggcgtca ccagcttgac cgtccaaatc atgacaattg
     2341 ttgagacagc gccgccaagt atcacatagg cccaaagcag cggacccggc gccagaaatg
     2401 tcgggagcgc caatcccaca agaataccgt tgaagccgta gagaccttcg gccaatgact
     2461 tttcgtccgc gcgaacccaa tacgccgtaa gcgttgctgc gaggagcgcc acgattcctc
     2521 caactgcaat ctgtggcgcg ccggcggcat aggcccccca gatgacggca gcgagaaaca
     2581 gcgcgccgct taatggattg tcctgaagca tgacttggcc ggtgccgcgc aggttgatgt
     2641 cgacgaagcg cagacccgca gacgacgcac acagcttttc ccagcctgcc aaaagttcct
     2701 tcattatcga ctcctgtaat gcagtgaact gtaatttggc gatgtgtccg ttctcgtttt
     2761 gcagttcagc gccagagaaa tggttggggg aggtcggcgc cagtcacctc cttgcgaaca
     2821 aggccccaaa actcgcgtat cttcgtcttc acctgcgcgc actcccggcc gacgactttg
     2881 aagatgaggc cggcatcgtt gggcagccgg caggcgccac atgccacacc gcgcgagagg
     2941 tcaatatcgt tctccacgcg cgcataaatg tgctcggctt tgtctttggg cgtgcacaag
     3001 atcacgttgc cgaatacatc gaacgagtcc atcacaccag tctgccgcat tgaatatcgc
     3061 tgcggctcga tgatcagctt ttcggtaaat agtagccgg
//
LOCUS       DASSRL010000018         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1470101, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000018 DASSRL010000000
VERSION     DASSRL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470101"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFL49_00315"
     CDS             <1..743
                     /locus_tag="VFL49_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7910450.1"
                     /translation="WASVLSLGKAPTVMSWTGAYAGLGLGARASRTDLQTTSATQGAV
                     LVNLTQAATSMPLDGTAFRASPYLGVNWQFAPRWVAGLEGDAGFAKQTKTLAGFPFSP
                     GVLAARRADDSLAVKTTWDAAVRGRLGFLLTPATLAYVTGGAAWQHYEVTSVCASPDV
                     CGVFGFAPVVIANSTTKAGWTAGGGIETALWGHWLARGEYRYSDFGAAPFSIARSSPI
                     PVLNPTVAAFEAKLRTHIVSFGLAYKFN"
     gene            complement(764..2407)
                     /locus_tag="VFL49_00320"
     CDS             complement(764..2407)
                     /locus_tag="VFL49_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HET7910451.1"
                     /translation="MSRGLDTFIYYATAPIRMLARSWYLRWGVAALCIVGFFFAATLW
                     ALNRFVPPADTGLAEALAILKPPPPLQPVTRASHVIAPVAVSLAAIGSALDSTTPREF
                     AGKNDNPVTQLLGQAEIGLTVSRGAMYATGQSNTLTITTPLNGSIRITGQIGNTAGKM
                     VGGLGGTIGGLIGGGAVGKQIDNLAGKALDQRTNFRGNVIVTSRPQLTAAWRIEPNLA
                     GRLDFSDAGTNIAGVKVNLANEIKPVLDPVVSNQIAALQSRLRNDPTIERTAREQWTK
                     MCRSIPLGGGKTGLPPLWLEMRPVRAAAAQPQVDARNVTLTIGVQAETRIVPNETKPN
                     CPFPAQLELVPPMQDGVLAVGVPIDMPFTELNKLLEMQLKGRHFPDDGSGPVDVEVLR
                     ATLSASGDRLLISLLVKAREKKSWFGFGTEATVHVWGKPVLDQQNQILRLNDISLAVE
                     SEAAFGLLGAAARAAMPYLQKALAEKAVIDLKPFANDARAKIAAALKDFQQVGDGVRV
                     DAAVNGLRLTGIEFDAKTLRVIAEANGTVKVAVTQLPRM"
     gene            complement(2494..>3136)
                     /locus_tag="VFL49_00325"
     CDS             complement(2494..>3136)
                     /locus_tag="VFL49_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910452.1"
                     /translation="SAADFKTVAIVASSPEALVTSPSNPAKNLAEFVKAAQGKTINFG
                     SAGVGSGSHIEAEYFFKKIAKIEATHVPFQGGAPAINATLGNQIDLLATTLGGGAAAQ
                     IAGGKLKGLGVASEKRVSVTPDVPTFAESGFPNFFAASWVGVFAPAKTDPQIIVTLNA
                     AINDTIRNPDVRKKLTDIGFDTIEGSPAQAEALFKSEVGKWGDMVKALNLSIK"
BASE COUNT          512 a          994 c         1034 g          596 t
ORIGIN      
        1 gttgggcgag cgtgctcagc ctggggaagg cacccacggt gatgtcgtgg accggggctt
       61 atgccggcct tgggctcggc gcgcgtgcgt cgcgcaccga cttgcagacc acatcggcta
      121 cccaaggcgc ggttttagtc aatttgaccc aggcggcaac cagtatgccg ctcgacggga
      181 ccgcattccg tgcgtccccc tacctcggcg tcaactggca gttcgcaccg cggtgggtcg
      241 ccggcctcga aggcgatgct ggttttgcaa agcaaaccaa gacgctggcc ggttttccgt
      301 tcagcccggg cgtgttggca gccaggcggg cggatgattc cctcgcggta aagacaacct
      361 gggacgcggc cgtgcgcggg cgtctcggtt ttctgctcac tcccgccaca ttggcctacg
      421 tcactggcgg cgcggcatgg cagcactatg aagtcacttc tgtctgtgcg agtcccgatg
      481 tctgtggagt tttcggtttt gcgccggtgg tcatcgcaaa ctctacaacc aaggcgggct
      541 ggacggccgg aggaggtatc gaaaccgcgc tctggggtca ctggctggcg cgcggcgaat
      601 atcgttattc ggattttggc gccgcaccgt tttccatcgc gcgatccagt cctattccgg
      661 tgctcaaccc gaccgttgcc gcgttcgagg cgaaattgcg tacccacatc gtgagcttcg
      721 gcctggccta caaattcaac taagcggctg ggaggctgga cggttacatc ctcggcaatt
      781 gcgtcaccgc aaccttcacc gtgccgtttg cttcggcgat cacgcgcagg gtcttggcgt
      841 cgaactcgat gccggtgaga cgaagtccgt tcaccgcagc gtcgacccgc acgccgtcgc
      901 caacctgctg gaagtccttg agcgccgcgg cgatcttcgc cctggcgtca ttggcaaaag
      961 gcttgaggtc gatcacggct ttttcggcga gcgctttctg cagataaggc atggccgcgc
     1021 gcgccgcggc gccgagcagg ccgaaggctg cctcggattc aaccgcgagc gagatgtcgt
     1081 tgagtcgcag gatctggttc tgctggtcga gcacgggctt gccccagaca tgcaccgtcg
     1141 cttccgtgcc gaagccgaac cagcttttct tctcgcgcgc cttcacgagc agagaaatca
     1201 gcagacgatc accggatgcg gagagggttg cacgcagcac ctcgacgtcg accggaccgc
     1261 tgccgtcatc ggggaaatgg cggcccttga gttgcatctc gagcagcttg ttgagctcgg
     1321 tgaacggcat atcgatcggc acgccgacgg ccagcacgcc gtcctgcatc ggcggcacca
     1381 gctcgagctg cgcggggaac gggcagttcg gcttggtctc attcggcacg atgcgcgtct
     1441 ccgcctgtac gccgatggtg agggtgacat tgcgcgcatc gacctgcggc tgcgctgccg
     1501 cggcgcgcac cggccgcatc tccagccaga gcggcggcaa accagtcttg ccgccgccga
     1561 gcgggatgga gcggcacatt ttcgtccatt gctcgcgcgc tgtgcgctcg atcgtcggat
     1621 cgttgcgcag gcgcgactgc agtgcggcga tctggttgct gacgacggga tcgagtaccg
     1681 gcttgatctc gttggcgagg ttgaccttga cgcctgcgat gtttgtgccg gcatcggaga
     1741 aatcaagccg gccggcgagg ttcggctcta tgcgccaggc ggcggtgagc tgcggccgcg
     1801 acgtgacgat cacgttgccg cggaaattcg tgcgctggtc aagcgccttc ccggcaagat
     1861 tgtcgatttg cttgccgacc gcaccgccgc cgatcagtcc gccgatcgtg ccaccgagcc
     1921 cgcccaccat ctttccggca gtgttgccga tttgcccggt gatgcgaatg ctgccgttga
     1981 gcggggtcgt gatggtcagc gtgtttgatt ggccggtggc atacatcgcg ccgcgcgaga
     2041 ccgtcagccc gatctcggcc tgaccgagca gctgtgtcac cgggttatca ttcttgccgg
     2101 cgaattcacg cggcgtcgtc gagtcgagcg cgctcccgat ggcggcgagc gagaccgcga
     2161 ccggcgcaat cacgtgcgag gcgcgcgtga ccggttgcaa cggcggcggc ggcttcagga
     2221 tggcgagcgc ttccgccaag ccagtgtcgg cgggaggaac gaaacggttg agcgcccaca
     2281 gcgtcgccgc gaaaaaaaat ccgacgatac acagggcggc gacgccccag cgaagatacc
     2341 acgaacgtgc caacatccgg atcggagccg tcgcgtagta gataaaagta tcgagccctc
     2401 ggctcatgcc cgtcccgccc gattcgccgc cgcgcgaatc ctaagccgat ggtcgcggct
     2461 tcgccagcgc cggaaatgtc gcgggcgctt gcgctacttg atcgacaggt tcagcgcctt
     2521 gaccatgtcg ccccatttgc cgacctcgga cttgaacaag gcctcggcct gcgcgggcga
     2581 gccctcgatg gtgtcaaaac cgatgtcggt gagcttcttg cggacgtccg gattcctgat
     2641 ggtatcgttg atcgcggcat tgagcgtcac aatgatctgc gggtcggtct tggccggcgc
     2701 gaagacgccg acccaagacg cggcaaagaa gttgggaaag cccgactcgg cgaacgtcgg
     2761 aacgtcgggc gtcaccgaca cgcgtttctc gctggcgacg ccgagcccct tgagcttgcc
     2821 gcccgcgatc tgcgcggcgg cgccgccgcc gagcgtcgtc gcgagcaggt cgatctggtt
     2881 gccgagcgtc gcattgatcg cgggggcgcc gccctggaac ggcacatgtg tcgcctcgat
     2941 ctttgcgatc ttcttgaaga aatattcggc ctcgatgtgc gagccgctgc cgacgccggc
     3001 cgagccgaaa ttgatggtct tgccctgcgc cgccttgacg aactccgcga gattcttggc
     3061 gggattgctc gggctcgtca ccagcgcttc ggggctcgac gcgacgatcg cgacggtttt
     3121 gaaatccgcg gccgag
//
LOCUS       DASSRL010000019         5787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_385457, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000019 DASSRL010000000
VERSION     DASSRL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385457"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..372
                     /locus_tag="VFL49_00330"
     CDS             40..372
                     /locus_tag="VFL49_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910453.1"
                     /translation="MTRLAILAAMAAISVGIQTRAAVADDVPTYDVRKSCKADVQAYP
                     TAGDTTGCLRDEQDARTTLVSQWGQFTPDSRVRCTQMVGDPAGPQSYVELLTCLQMAR
                     DVKTLPKN"
     gene            414..1109
                     /locus_tag="VFL49_00335"
     CDS             414..1109
                     /locus_tag="VFL49_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET7910454.1"
                     /translation="MTTGGKSRLGQLQERHSDQLLTVLTVLLLVMMFVVAPVQAAGIS
                     IVGIEAFGVLVALVMVAGVLVISASPAITFVILCGFLLNIVVVIARLTNPLSYDLYFV
                     SIAWLIIVGTLGFVVGRKVFEQGQVTVHRIIGAVLLYLLIALAFVCLFMLVAFRVPNA
                     FNGLVLEDNQKLASNFIYFSFVTLTSTGYGDIYPVHPVARSLCNLETIIGQLYPATLL
                     ARLVSLEIEGRRR"
     gene            1313..1990
                     /locus_tag="VFL49_00340"
     CDS             1313..1990
                     /locus_tag="VFL49_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HET7910455.1"
                     /translation="MTKRFMLAAAACALAFASALPSFAADMPRGRYAEPAPSYFSWSG
                     FYVGLNAGYGWGTSDWTGGITSGSTSPSGGLFGGTIGFNVQNWGLVFGVEGDAAGNWM
                     RNDTSSGTGICAVPGCEVQTSWFGTARGRVGYAFDRALFYVTAGGAFGDVQMTAGGAT
                     ATVSRAGWTAGVGLEYGILGPWSAKIEYLYADLGNATCGAVACGVDTTVSFKTSIVRL
                     GVNYRFW"
     gene            complement(2001..2876)
                     /locus_tag="VFL49_00345"
     CDS             complement(2001..2876)
                     /locus_tag="VFL49_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018243453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7910456.1"
                     /translation="MLTRRGLITAGAGLGIGGLMLSSTGAYAAAQAANDLVITDYRLS
                     PPAWPKGRRLTISAIADLHAGGPNMGISRVRQVVDAANGLGSDLVVVLGDYFATHRFV
                     TERVPPPAWAAELARLRAPLGVYAILGNHDWWFGIDGVRNALRNVGVPLLRNSVVHVG
                     SPDQRIWLAGLDDQLAHWLGPSRFRGEDDLPGTLARINTDDPVILLAHEPDIFVDVPA
                     RVALTIAGHTHGGQIRLPFGPPIWVPSAYGRRFAYGHIVESNRHMVVSGGLGCSGVPL
                     RLGVPPEIVRITLGA"
     gene            complement(2987..3772)
                     /locus_tag="VFL49_00350"
     CDS             complement(2987..3772)
                     /locus_tag="VFL49_00350"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HET7910457.1"
                     /translation="MRLLIVILWLAGAGGGIAVAREVPLPRPRPPIWTEPQSFREIAG
                     PDFDSTGITSEPTECDKRIRTIAVVESLPRLIGPGACGGSDMLRLEAALRPDGARIEL
                     RPAPVLRCEFAESVAAWLRDEAAPRVDKLGALLRAVETFDDFECRGRNRVSGAKLSEH
                     GKGNAVDLRAFLLADGRSLGLTDISVAKDFREEIRDSACHRFTTVLGPGADAYHESHI
                     HLDLLERRQGYRMCQWDVREPPKTEIVAQVPLPPPRPAVPVAR"
     gene            3947..5587
                     /locus_tag="VFL49_00355"
     CDS             3947..5587
                     /locus_tag="VFL49_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HET7910458.1"
                     /translation="MHGLMQDWPLLCHRIIDHAATFHGERKVISRSVEGPMHTTTYAQ
                     VRARALKVAQRLDKDGVRLGDRVATLAWNTWRHLEAWYGILGVGAVYHTVNPRLFPEQ
                     IAWIINHAGDRVMITDLTFVPLLEKIADKLPTIERYIVLTDAAHMPKTTLKNAVAYED
                     WIGNVDGDFVWKSFDENTAAGMCYTSGTTGHPKGVLYSHRSNVLHAFMASLPDCKGLS
                     SRDVVLPVVPLFHANGWSLAFSTPMVGASMVMPGAKLDGASIYELLNTYKVTFTAAVP
                     TVWLMLLQDLEKTGGKLPHLKRVVIGGSACPRAMTRTFQDKYGVEVVHAWGMTEMSPL
                     GSLCTMKPEYAGLSGEAKLDIEMKQGHPPFGVEMKITDDHGKDLPWDGKTFGRLKVRG
                     PAVARSYFKVDDDAVFESDGWFDTGDVATMDAYGYMQITDRAKDVIKSGGEWISSIDL
                     ENLAVGHPKVAEAAVIGVSHPKWDERPLLVIVLKKGESAAKDDILGFMRGKVANWWMP
                     DDVVFVDEIPHTATGKIQKLTLRQQFKDYRLPTAQAAE"
BASE COUNT         1104 a         1781 c         1849 g         1053 t
ORIGIN      
        1 tgtttgtttc gacgagccac acgtgctcgg gaggcgacta tgacaagact tgctatattg
       61 gccgctatgg cggcaatttc cgtcggtatt cagacgcgcg ctgcggttgc cgacgacgtg
      121 ccgacttatg atgtccgcaa gagctgcaaa gccgacgtgc aggcttatcc gactgccggc
      181 gatacgacag gttgcctgag ggatgagcag gacgcgcgga cgacgcttgt cagccagtgg
      241 ggccaattta cacccgacag cagggtgcgc tgcacccaga tggtcggcga cccagcgggc
      301 ccacagagct acgtcgagct tctgacctgc cttcagatgg cacgggatgt gaagaccctg
      361 ccgaaaaact gaacgcaagc ggtgcatcgg acgggagcaa aggtcggcgc gcaatgacga
      421 ccggcggcaa atcccgtctc ggccagttgc aggagcggca tagcgatcag ctgcttaccg
      481 ttctgacggt tcttttgctc gtgatgatgt ttgtcgtcgc gccggtgcag gctgccggaa
      541 tctccatcgt cgggatcgag gccttcggcg tgctcgtcgc actggtcatg gtcgccggcg
      601 tgctggtcat ttcggcaagc ccggcgataa cgttcgtcat tctctgcggg tttttgttga
      661 atattgtggt ggtcattgca cggctgacca acccgctcag ctacgacctc tatttcgtgt
      721 cgattgcctg gttgatcatt gtcggcacgt tgggttttgt cgtgggacgg aaagtctttg
      781 aacaaggaca ggttacggtc catcgcatta tcggagccgt tctgctctat cttttgattg
      841 ccctcgcctt tgtgtgcctg ttcatgctgg tcgcgtttcg cgttccgaat gcgttcaatg
      901 gccttgtcct ggaggacaac cagaagctgg caagcaactt tatctacttc agcttcgtca
      961 cgctgacgtc gaccggatat ggcgacatct accccgtgca tccggtggcc cgcagcctgt
     1021 gcaatctgga aacgatcatc ggccagcttt atccggcgac attgcttgcc cggctggtca
     1081 gcctcgagat cgagggacga cgccgatgaa agacgcgcga tttctcatcg ccagtcccta
     1141 aaaagccggg tcaaatcgcc ggctcgcaac gattcgttaa ctacgaatct ggccttctac
     1201 cggcgctcgc ctcccgaatt tgcgtatcgg tttgtcgcgt cacgattgcg gtccttcgca
     1261 gatcgtcatc cgcgaatgcc gcactttgag ccggcacata ccaggagatc ccatgaccaa
     1321 acgattcatg cttgctgcag cggcgtgcgc cctggctttc gcatcggcct tgccgtcatt
     1381 cgcggccgat atgccgcgcg gacgatacgc cgagcctgct ccctcctatt tcagctggag
     1441 cggattttat gtcgggctca acgccggata cggctggggc acatccgact ggaccggcgg
     1501 cataacgtcc ggctcaacca gcccgtcggg aggcctgttc ggcggcacca tcggtttcaa
     1561 cgtgcagaac tggggcctgg tcttcggcgt tgagggcgac gccgccggca actggatgcg
     1621 gaacgacacc agttcgggaa ccgggatctg cgcggtgcca ggctgcgaag tgcaaaccag
     1681 ctggtttggc actgcgcgcg ggcgcgtggg ttacgccttc gaccgcgcct tgttctacgt
     1741 caccgcgggc ggcgccttcg gcgatgttca gatgacggcc ggcggcgcga ccgcgacggt
     1801 gagccgggcc ggctggaccg cgggcgtggg ccttgaatac ggaatcctcg gtccctggtc
     1861 ggcgaagatc gaatatcttt atgccgacct cggcaacgcg acgtgcggcg ccgtcgcctg
     1921 cggcgtcgac acgaccgtga gcttcaagac cagcatcgtg cggcttggtg tgaactatcg
     1981 cttctggtag ccgcccgaca tcacgcgccc agcgtaatcc gcacgatctc cggcggcaca
     2041 cccaggcgca gcggcacgcc gctgcagccg aggccgcccg agacgaccat gtggcggttg
     2101 ctctctacaa tgtggccata agcgaaacgc ctgccgtaag ccgacggcac ccaaatcggc
     2161 ggcccaaaag gcaggcgaat ttggccgcca tgggtgtggc cggcgattgt cagcgcgacg
     2221 cgggccggca cgtcgacaaa aatatccggc tcgtgcgcaa gcagaatgac ggggtcatcg
     2281 gtattgatgc gcgcaagcgt gccgggcaga tcgtcttcgc cgcggaagcg actggggccg
     2341 agccaatgcg caagctggtc gtcgaggccg gccagccaga tacgctgatc cggcgagccc
     2401 acatgcacga cgctgttacg caacagcggc acgccgacat tgcgcagcgc gttgcgcacg
     2461 ccgtcgatgc cgaaccacca gtcgtgattg ccgaggatgg cgtagacgcc gagcggcgcg
     2521 cgcaggcgtg ccagctcggc cgcccaggcc ggcggcggca cccgttcggt gacgaaacgg
     2581 tgcgtcgcga aataatcgcc aagcacgacc acgagatcgc tgcccagccc attggccgca
     2641 tcgaccacct gccggacgcg actgatgccc atattggggc cgccggcatg caaatcggcg
     2701 attgccgaaa tggtaagccg ccggcccttc ggccaggccg gcggagacag gcgataatcg
     2761 gtgatcacca ggtcgttcgc ggcctgtgcg gctgcgtagg cgccggtcga agacagcatc
     2821 agcccgccaa taccgaggcc ggcgcccgcc gtgatcaggc cgcgccgggt gagcatgcgc
     2881 ggctcaagat ggccacgcgc gatggggtca ttcgtcgtca tgggagtttg ctagccgcaa
     2941 cctatgaacg gagggtggat ttgcaatcgg aatttcgtcg catggtttac cgggcgacag
     3001 gaaccgccgg gcggggcggc ggcagcggaa cctgggcgac aatttccgtt ttgggaggct
     3061 cgcgcacgtc ccactggcac atccgatagc cctgcctgcg ctcgagcagg tcgagatgaa
     3121 tgtgagactc gtgataggca tccgcgccgg gaccaagcac cgtcgtgaag cgatggcagg
     3181 cgctgtcgcg gatctcctcg cgaaaatctt tcgccactga aatatcggtg agcccgagcg
     3241 aacggccgtc cgccaacaag aatgcgcgga gatcgaccgc gttgcccttg ccgtgttcgc
     3301 tcaacttcgc accggaaacg cgattgcggc cgcggcactc gaaatcgtca aaggtctcga
     3361 cagcgcgcag cagggcaccg agcttgtcga cgcgcggcgc cgcctcatcg cgcagccagg
     3421 cggcgacgga ttcggcaaat tcgcagcgca ggaccggcgc cggtctcagt tcgatgcggg
     3481 caccatcagg ccgcagcgcc gcctcgagcc gcagcatatc gctgccgccg caggcgccgg
     3541 gaccgatcag ccgtggcagc gactctacga cggcgattgt ccggatgcgc ttgtcgcact
     3601 ccgtcggctc gctggtgata cctgtcgagt caaaatcagg gccggcaatt tcgcggaagg
     3661 attgcggctc cgtccagatt ggcggacgcg ggcgcggcag cggcacctcc ctggcaacgg
     3721 caataccgcc gccggcaccg gccagccaga ggatcacaat caaaagacgc atgaaatgga
     3781 tgtctgccgc caatacggca tttataggag ttcgctatgc aaccgagcag gcaggccgga
     3841 cgtatctcga caggaaaccg ttgcaccgcg cgacccgccg gataaccttg agccgggctg
     3901 gcgaggggcg ccggccggaa aggcgaatat ccaggagggg aatgtcatgc acggcctgat
     3961 gcaggattgg ccactgctgt gccatcgcat catcgaccat gccgccacgt ttcatggcga
     4021 gcgcaaggtc atctcgcgct cggtggaagg gccgatgcac accacgacct atgcgcaagt
     4081 gcgcgcccgc gcgctgaaag tcgcgcaaag gctggacaag gacggcgtca ggcttggcga
     4141 tcgggtggca acccttgcat ggaatacctg gcggcacctg gaggcctggt acgggattct
     4201 cggcgtcggc gccgtttatc acacggtcaa tccgcggttg tttcccgagc agatcgcctg
     4261 gatcatcaat cacgccggcg accgcgtgat gataaccgat ctcaccttcg tgccgttgct
     4321 ggagaagatc gccgacaagc tgccgaccat cgagcgctac atcgtgctca ccgatgcggc
     4381 gcatatgccg aagacaacgc tgaaaaatgc ggtcgcctac gaggactgga tcgggaacgt
     4441 cgacggcgac ttcgtctgga aaagtttcga cgagaatacc gcggccggca tgtgttacac
     4501 gtccggcacg accggacacc cgaagggcgt gctctattcg catcgctcga acgtgctgca
     4561 tgctttcatg gcgtcgctgc cggactgcaa gggcctttcc tcgcgcgatg tcgtgctgcc
     4621 ggtggtgccg ctgtttcacg ccaacggctg gtcgctcgca ttttccacgc cgatggtcgg
     4681 tgcctcgatg gtcatgccgg gcgcaaagct tgacggcgcc tcgatctacg aattgctcaa
     4741 tacgtacaaa gtcacattca ccgcggcggt gccgacggtg tggctgatgc tgttgcagga
     4801 tctcgaaaag accggcggca agctgcccca tctcaagcgg gtcgtgatcg gcggatcggc
     4861 gtgcccgcgc gcaatgaccc ggaccttcca ggacaaatac ggtgtcgagg tcgtgcacgc
     4921 ctggggcatg accgagatga gcccgctcgg ctcactctgc acgatgaagc ccgaatatgc
     4981 cgggcttagc ggcgaggcga agctcgatat cgaaatgaag cagggtcacc cgcccttcgg
     5041 cgtcgagatg aagatcaccg acgaccatgg caaagatttg ccgtgggacg gcaagacctt
     5101 cggccggctg aaagtgcgcg gaccggcggt cgcgcgcagc tacttcaagg tcgacgatga
     5161 cgccgtgttc gagagcgatg gctggttcga cacgggcgac gtggcgacga tggatgcgta
     5221 cggctatatg cagatcaccg atcgcgccaa ggacgtcatt aagtcaggcg gcgaatggat
     5281 ttcctcgatc gacctcgaaa acctcgcggt cggccacccg aaagtagctg aagctgcggt
     5341 gatcggcgtt tcgcatccca agtgggacga gcggccattg ctcgtgatcg tgctcaaaaa
     5401 gggcgagagc gcggccaagg atgacatcct cggcttcatg cggggcaagg tcgcgaactg
     5461 gtggatgccg gacgatgtgg tttttgttga cgagattccg cacacggcga ccggaaaaat
     5521 ccagaagctc acgctgcgcc agcaattcaa ggactaccgc ctgccgaccg cgcaggcggc
     5581 ggagtagtcc tcaactactc attcccgcga aagcaggaac caaacccagc gctgcacgga
     5641 cggcggcctc tcgctgggtc cccgctttcg cggggacgag cggaaattga gtcgctgccc
     5701 tctccccgct cattcccgcg aaagcgggaa tccagccgtc tttacggcga cgagagatct
     5761 cgccacagat ctctccaata cggatta
//
LOCUS       DASSRL010000020        11267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_280485, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000020 DASSRL010000000
VERSION     DASSRL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280485"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFL49_00360"
     CDS             <1..934
                     /locus_tag="VFL49_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7910459.1"
                     /translation="GNVKDWFRNNQPVKETGYVTHLWGSDAVAQINAHDSKTPLFLYL
                     AFTAPHAPYQAPKEYFDKYKQIADPTRRAYAAMVTAMDDEIGQVVAALEKKKMRENTL
                     IVFMSDNGGNRTAMLSGDVDVSKLKLPADNGPYRGGKGMLYEGGTRVVALANWPNRIK
                     SGEVKEVMHVVDMFPTLAGLAGGNLKKGKPLDGVDVWSTVSDGRPSPRKEVVYNIEPF
                     RGGVREGDWKLIWRTPLPSALELYNIAQDPFEKTNVADKNPQIVKELQKRIEELARES
                     AKSLFLVDAFTAVKASTHGPPALPSEDTFFTQGD"
     gene            complement(973..1518)
                     /locus_tag="VFL49_00365"
     CDS             complement(973..1518)
                     /locus_tag="VFL49_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7910460.1"
                     /translation="MALTRQSFRTALGALALAAGIVALQPGPARSAGDAPTGSVSGLP
                     IPRFVSLKSDRVNVRSGPNKDQEVRWVYTHAGMPVEITAEFENWRRVRDWEGAEGWVY
                     HSLLSGKRTAMVRPKAKDELVPLYENADIESAVTARLQSGVLGSLKSCNGNWCQFSGK
                     GFEGYIRQERLWGAYPNEKVE"
     gene            1636..2637
                     /locus_tag="VFL49_00370"
     CDS             1636..2637
                     /locus_tag="VFL49_00370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET7910461.1"
                     /translation="MAKTKRPLVIVTRKLPDSVELRMRELFDTRLNPDDTPMTQGQLA
                     EAVKTADVLVPTVTDRIDASVLSKSGEQLKLIANFGNGVDNIDVATAIQRGITVTNTP
                     GVLTEDTADMTMALILAVPRRLVEGSHVLTADKDWTGWSPTWMLGHRIWGKRLGIIGM
                     GRIGQAVARRARAFGLQIHYHNRRRVAPKIEQELDATYWESLDQMLARMDIVSVNCPH
                     TPATYHLLSARRLKMLRPEAYIVNTSRGEVIDENALARLIEAGDIAGAGLDVFEHEPA
                     VNPKLVKLARGGKVVLLPHTGSATIEGRVDMGEKVIINIRTFMDGHRPPDRVLPSML"
     gene            complement(2620..3423)
                     /gene="moeB"
                     /locus_tag="VFL49_00375"
     CDS             complement(2620..3423)
                     /gene="moeB"
                     /locus_tag="VFL49_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET7910462.1"
                     /translation="MLDASELERYARHIVLREVGGPGQAKLKAARVLVVGAGGLGAPV
                     LLYLSAAGVGTIGVVDDDSVSLSNLQRQVIFATRDIGKPKVERAASAMSSLNPHVSVR
                     THHVRLTAANALDLIANYDIVADGSDNFATRYLVSDACYFAKKPLVTAALGTFDGSLT
                     SIRAHEMSASGKPNPTYRCLFPEPPPPGTVPACAEAGILGALPGVLGALMALEAIREI
                     VGFGEGLVGRLLMVDARSMRFETLTYAWDPNNPLSGKKPTIKDLSQHRW"
     gene            3565..4998
                     /locus_tag="VFL49_00380"
     CDS             3565..4998
                     /locus_tag="VFL49_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET7910463.1"
                     /translation="MHVSGPTAALALLLGVAIAAAQESPGIQPAPAPADVKPTPPRAA
                     KPAKAVAPKISAPATVSAKTAAVVSEPAKPEPPTEPALSAGERLKIRSALLWSGDYTA
                     SIGGEDPLVSAIKNYQKRAKSRITGVLSSTERTNLLTAAQSHEDEFGWTVVVDPATGV
                     RIGLPMKLVPQARDTPRGTRWSAKHGEVQVETFRIKNSEVKLATLFEQQKKEPSTRKI
                     EQSALRDDNFVITGMQGLKKFSVRAYARDGEIRGVTVLFDQMMETIVAPVTAAMVSAF
                     SPFPERSLPFAAPAKSVEYGTGLVVSARGHIITDRKLASGCQIIIADDLGHADRIAED
                     HDNGLALLRVYGVRELSPLALAANTARKGDLTLVGIPDPKEQNGGKRPTEIKARLAEN
                     NAIELRQPVPMAGFSGAAALDAQGQVLGMMEMRNFVLASIEPAAPPVRLISAEKIRAF
                     LGEYNVPLASHNADARSAVVRVICVRK"
     gene            complement(4999..5772)
                     /locus_tag="VFL49_00385"
     CDS             complement(4999..5772)
                     /locus_tag="VFL49_00385"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7910464.1"
                     /translation="MTYENIVVETKGRVGIIRLNRPHALNALNKALIAELTKAIEGFD
                     ADDKIGCMLITGSDKAFAAGADIKEMADKPFIEAYLGDFVSDWNAAAKARKPIVAAVA
                     GFALGGGCELAMQCDIVIAADTAKFGQPEIKLGVIPGIGGTQRLTHAIGKAKAMDLIL
                     TGRMMDAAEAEKSGLVARVVPAARLLEEAMKVADTIANMSLPSAMAGKEAVNQAFESG
                     LSEGIAFERRIFHSLFATEDQKEGMKAFVEKRPPKWKHK"
     gene            complement(5800..6762)
                     /locus_tag="VFL49_00390"
     CDS             complement(5800..6762)
                     /locus_tag="VFL49_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7910465.1"
                     /translation="MRKVLMIALTLAATLVTASAADYPDHPVRLIVPQAPGSATDNAA
                     RLLGAVLTQELGQQIIVENRPGGALTIGLDLVAKSPPDGYTIGMGPIGALAITRHMVA
                     KLPYDIERDFQPIALVTRGQMLLAVSPKLPVNSVKELIDYARQNPGKLTNASSSNGSP
                     GHVGAELLKHMTGTKIVHVPYRGGSLAINDLIAGHVDLMFESLQSIAPFGRDGKVRAL
                     AVTGDHRSPSFPDLPTVAEAGVPGYVAATWTGVIAPAAVPRPIIDKLNAAINRVLKSE
                     AFLEKFSKFGDEPGGGTPEEFAATIKADSAKWADVVKRSGAKLD"
     gene            complement(6796..7662)
                     /gene="mutM"
                     /locus_tag="VFL49_00395"
     CDS             complement(6796..7662)
                     /gene="mutM"
                     /locus_tag="VFL49_00395"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET7910466.1"
                     /translation="MPELPEVETVRRGLAPAMEGERFDKVEVRRGDLRWPLPKGFAEK
                     LHGKLVKGLGRRAKYLLADLSSGDVLLMHLGMSGSFRVGKNSTPGKYHHERSRSTAHD
                     HIIFHMSNGVTVTFNDPRRFGSMKLVARARLEQEPLLRSLGPEPLGNAFDAAMLAKAC
                     VGKRTSLKAALSDQRVVAGLGNIYVCEALFRARLSPKRQASTLADRNGRPNERAQNLV
                     EAIKAVLMDAIEAGGSSLRDHRRADGSLGDFQHNFRVYDREGAPCPGCKGRIKRIVQS
                     GRSTFYCPSCQK"
     gene            7741..8499
                     /gene="ubiE"
                     /locus_tag="VFL49_00400"
     CDS             7741..8499
                     /gene="ubiE"
                     /locus_tag="VFL49_00400"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET7910467.1"
                     /translation="MPTDGEQTHFGYRSVPLDGKQRRVDDVFHSVARRYDLMNDLMSG
                     GLHRAWKDALVTAINPPRSERNFRALDLAGGTGDVAFRVIEAGGHGTRVTVCDINPDM
                     LEVGRERAAARGIDRVVFEQGNAERLAYPDRSFDCVTIAFGIRNVPRIEHALEEAHRI
                     LRIGGRFLCLEFSSVDVPGIDTLYELYSFQVIPRLGEIVTGDHDAYQYLVESIRKFPK
                     PKVFARMLETAGFRRVSFTPMTGGVVGLHSGWRL"
     gene            8496..10067
                     /gene="ubiB"
                     /locus_tag="VFL49_00405"
     CDS             8496..10067
                     /gene="ubiB"
                     /locus_tag="VFL49_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HET7910468.1"
                     /translation="MITAFSHLLRLARAGFVFAREGVFALVDTRPLPVPAKTAIALAR
                     LLERPSAKHGENRLAAALTRLGPTYVKLGQFLATRPDVVGAAIARDLESLQDKMPPFP
                     QSEAEAAVVAAFDKPLSALYASFGSVVAAASIAQVHRAEVEANGERKAVAVKILRPGI
                     ERRFKSDLDAFFFAARNAEAFSAEARRLRLIEVVSTLARSVAIEMDFRLEAAAASEMA
                     ANTREDVDFRVPAVDWDRTAREVMTLEWIDGIRLSDHGSLRARGFDLPELGRNVIQSF
                     LRHALRDGFFHADMHPGNLFVDDDGKLVAVDFGIMGRLGPKERRFLAEILLGFITRDY
                     RRTAQVHFDAGYVPRHHSVESFAQAIRAIGEPIHNRTAEDISMAKLLMLLFEVTGLFD
                     MRTRPELLLLQKTMVVVEGVARSLDPKLDMWGTADPVVREWIARNLGPVGRLEDAVEG
                     AGEIGRFFGEVPGLLTRAGTLVDQFDDITRDGLVLSPETVSAIGAAEARRNRWTAIAL
                     WVIAILLAFLIWRLI"
     gene            complement(10481..10840)
                     /locus_tag="VFL49_00410"
     CDS             complement(10481..10840)
                     /locus_tag="VFL49_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HET7910469.1"
                     /translation="MTYVDGFVLPVKKRQLATYRRMAKQAGKVWRQHGALEFHECVAD
                     DVPHGKRTSFPRSVKLKRGEIVVFSYIVYRSRGDRDRVMTKVMKDKRIDAMMKSKKIP
                     FDGKRMIFGGFKTMVSA"
     gene            complement(11002..>11267)
                     /locus_tag="VFL49_00415"
     CDS             complement(11002..>11267)
                     /locus_tag="VFL49_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7910470.1"
                     /translation="NKVMHASLKIGDARVMASDGENKGNPDFKGFALSLDARDEADAD
                     RMFNALSSGGKVTMPLTKTFFSPRFGMLADKFGVNWMILVHPK"
BASE COUNT         2195 a         3413 c         3384 g         2275 t
ORIGIN      
        1 gggcaacgtg aaagattggt tccgcaacaa ccagccggtt aaagaaaccg gctacgtgac
       61 tcacttgtgg ggcagcgatg cggtcgcgca gataaacgca cacgattcaa aaacgccatt
      121 gtttctctat ctcgccttca cggcgccgca tgctccgtat caggccccga aggaatattt
      181 cgacaaatac aagcaaatcg ctgatccgac gcgacgcgct tacgcggcca tggttaccgc
      241 catggacgac gagatcggtc aggttgtcgc cgcccttgaa aaaaagaaaa tgcgcgaaaa
      301 cacgctgatc gtcttcatga gtgacaacgg cggcaaccgt accgccatgc tctcaggaga
      361 tgtcgatgtg tcgaagctga aattgccggc cgacaacggc ccgtatcgag gagggaaagg
      421 aatgttgtac gagggcggca ctcgggttgt cgcactcgcc aactggccga accggattaa
      481 atccggcgaa gtcaaagaag tcatgcacgt ggtagacatg tttccgacgc ttgccggact
      541 cgcaggaggt aacctgaaaa agggaaaacc acttgatgga gtcgatgttt ggtccacagt
      601 tagcgacggc aggccttcgc cgcgtaagga agtcgtctac aacatcgagc cattccgtgg
      661 cggggtgcgg gagggcgact ggaagctcat ctggcgaact ccgttgccgt ccgctttgga
      721 actttacaac atcgcgcaag acccttttga gaagaccaac gttgccgaca aaaatccaca
      781 gatcgtgaag gaactacaaa agcggattga ggaactggca cgggaaagcg ccaaatcgct
      841 ttttctcgtt gatgcattca ctgcggtcaa ggcttccact cacgggcctc cagcgttgcc
      901 aagcgaggat acgtttttta cgcagggtga ctagatgccg ccgccccata aacatgccgg
      961 caattgaacg agttactcca ccttttcgtt cggataggcg ccccaaaggc gctcttgccg
     1021 gatatatcct tcaaagcctt tcccgctgaa ctggcaccag ttgccgttgc aggacttgag
     1081 cgacccgagc acgccggact gcaagcgggc ggtgacggcc gactcgatat ccgcgttctc
     1141 gtatagcggc acgagttcgt ccttggcctt tggccgcacc attgccgtgc gcttgccgga
     1201 caacaatgag tgatagaccc agccttcggc accctcccag tcccgaacac ggcgccagtt
     1261 ttcgaattcg gcggtaatct cgaccggcat gccggcatgg gtatagaccc accgtacctc
     1321 ctggtctttg ttcgggccgg aacgcacatt gacccgatcg gatttgaggc tgacgaaacg
     1381 cgggatcggg aggccgctga ccgagccggt gggagcgtcg cctgccgacc gggcaggacc
     1441 gggctgcaat gccacgatgc cggccgccaa tgccaaggcg cccagcgcgg tccggaagct
     1501 ttgtcttgtc agggccatct ggtaggaaaa agcatgggat ttcggctgac acgcagtgtc
     1561 ggcgaccacg gttaaagagg gctaaatcgg cgcttccttg gcggtaccac accaaatttc
     1621 cgcagttgag aatcgatggc gaagacgaaa aggccgctgg ttatcgtgac gcgcaagctc
     1681 cccgattctg tggagctgcg gatgcgggaa ctttttgata cccgcctcaa tcccgacgac
     1741 acgccgatga cgcaaggcca gctcgccgag gcggttaaga ccgccgatgt cctggtgccg
     1801 acggtcaccg accggatcga tgcatcggtg ctcagcaagt cgggggaaca gctcaagctc
     1861 atcgccaatt tcggcaatgg tgtcgataac attgacgtgg cgacggccat tcaacgtgga
     1921 ataacggtca ccaatacgcc gggcgtcctc accgaagaca ctgcggacat gacgatggcg
     1981 ctgatcctcg cggtgcctcg ccggctggtg gaaggctcgc acgtcctgac ggccgacaag
     2041 gactggaccg gctggtcgcc gacctggatg ctcggccacc gaatctgggg caagcgtctc
     2101 ggcatcatcg gtatgggacg catcggtcag gccgttgcgc ggcgagcccg cgcgttcggg
     2161 ctgcagattc actatcacaa caggcgacgg gtcgcgccca aaatcgagca ggaactcgac
     2221 gcgacttatt gggaaagcct cgaccagatg ctggcccgca tggacatcgt gtcggtcaat
     2281 tgcccgcaca cgccggcgac ctatcatctg ctttcggcgc gccgtctcaa aatgctgcgg
     2341 ccggaggctt acatcgtgaa cacgtcgcgc ggcgaggtga tcgatgagaa cgcgctcgcg
     2401 cgtctgatcg aggctggcga catcgccggc gccggcctgg acgtgttcga gcatgagcct
     2461 gcggtgaatc caaagctggt gaagctggcg cgtggtggaa aggtcgtgct cttgccgcac
     2521 acaggatcgg cgacgatcga aggccgcgtc gacatgggcg agaaggtcat catcaacata
     2581 cgaaccttca tggacgggca tcgaccgccg gatcgtgtgc taccatcgat gctgtgacag
     2641 atccttgatt gtcggtttct tgccgctgag cggattgttc ggatcccacg cataggtcag
     2701 cgtctcgaaa cgcatcgagc gggcgtcaac catgagaagg cggccgacca ggccttcgcc
     2761 aaagccgacg atctcgcgga tcgcctccag cgccatcagc gcgccgagca cgcctgggag
     2821 cgcgccgaga ataccggcct cggcgcaggc aggcactgtg ccgggtggag gcggttcggg
     2881 aaacaagcaa cggtaggtgg gattcggctt accgctcgcg gacatctcgt gcgcccgtat
     2941 ggatgtgagc gatccgtcga atgttccaag ggcggcggtg accagcggct tcttcgcgaa
     3001 atagcaggcg tcggaaacaa gatagcgggt cgcgaaatta tcggaaccgt cggcgacgat
     3061 atcgtagttg gcaatcaagt cgagcgcgtt cgctgctgtc agtcgcacgt gatgcgtcct
     3121 gacgctgaca tgtggattga gactgctcat cgcactggcg gcgcgctcga cttttggctt
     3181 gccgatatcc cgggtggcga agatcacttg ccgctgtaga ttcgagagcg atacgctgtc
     3241 gtcgtcgacg acgccgatcg ttccgacgcc ggccgcagaa agatagagca gcaccggtgc
     3301 gccaagaccc ccagcgccca cgacaagcac tcgcgcggcc ttgagcttcg cctggccggg
     3361 accgcccacc tctcgcagca caatgtggcg ggcataacgt tcgagttcgc tggcatcgag
     3421 catcgtggtt aaccctatag ggtggcgtaa ggctttgggc cagtcctcca gcggatttgc
     3481 acggcggtgc gcggtcccca ggtccatttt gcggatcgat cgccatcagc taaaatagca
     3541 aaaatccgtg gggacggcgt tacgatgcac gtgtcagggc cgacagcggc gcttgcgctt
     3601 ttgctcggcg tcgcaattgc ggcggcgcag gaatcgcctg gaattcaacc ggcgcccgcg
     3661 ccggcagacg tgaaaccgac gcccccgcgc gcggccaaac cggcgaaagc ggtcgcgccg
     3721 aaaatttccg cgcccgcaac ggtctcggcg aaaaccgctg ctgttgtatc tgagcccgcc
     3781 aagcccgaac cacccacaga gccggcgctt tccgccggcg agcggctcaa gattcggtct
     3841 gcattgctct ggtccggtga ttacaccgcg tcgatcggcg gcgaggatcc gcttgtctcc
     3901 gcaatcaaga attatcaaaa acgcgcaaag tccaggatca cgggagttct ttcctccacc
     3961 gagcgaacga atttgctcac cgccgcccag tcgcacgaag atgaatttgg atggacggtc
     4021 gtcgtcgatc cggccaccgg cgtgcgcatc gggctgccga tgaagctggt gccgcaggct
     4081 cgcgatacgc cgcgaggaac gcgctggtcg gcgaagcacg gtgaggtgca ggtcgaaacc
     4141 ttccgtatca agaattccga agtcaagctc gccacccttt tcgaacagca aaagaaagaa
     4201 ccctcgacgc gcaagatcga acaaagcgcg ctgcgcgacg acaatttcgt cattaccggc
     4261 atgcaaggtc tgaagaaatt ctccgtgcgc gcctatgccc gcgatggcga gattcgtggt
     4321 gtcacggtgc ttttcgatca gatgatggaa acaatcgtcg cacccgtgac ggcggcgatg
     4381 gtcagcgcat tctcgccgtt tcccgagcga agcctgccgt tcgccgcgcc ggcaaaatcg
     4441 gtcgaatatg ggaccgggct ggtcgtgagc gcccgcggcc atatcatcac agatcggaaa
     4501 cttgcgtcgg gttgccagat catcattgcg gatgatcttg gccatgccga tcgtattgcc
     4561 gaggatcacg acaatggcct ggcgctattg cgcgtttacg gggtgcgcga gctgtctccg
     4621 ctcgcgctcg cagcgaatac cgcacggaag ggcgatctga cactggtcgg catccctgac
     4681 ccgaaagaac agaatggcgg caaaagaccc acggaaatca aggcgcgtct cgccgagaac
     4741 aatgccattg aactgcgtca gccggtgccg atggcgggct tttcaggcgc cgctgcactc
     4801 gatgcgcaag gtcaagtcct cggcatgatg gagatgcgca atttcgtgct cgccagtatc
     4861 gaaccggctg caccaccggt gcggctgatt tcggcggaaa agattcgtgc ttttcttggc
     4921 gaatacaacg tgccgcttgc ttcgcacaac gcggatgcca gatcggccgt cgtgcgagtt
     4981 atctgcgtcc gcaaataact atttgtgctt ccacttcggc ggacgttttt cgacaaaagc
     5041 tttcatgcct tccttctggt cttctgtcgc aaacagcgag tggaaaattc ggcgctcgaa
     5101 agctatgcct tccgaaaggc cactttcgaa agcctgattg actgcttcct tgcccgccat
     5161 cgcagacggt aacgacatgt tcgcgatggt atccgcgacc ttcatggctt cctccagcaa
     5221 acgcgccgcc ggcacgacgc gcgccaccag cccggacttc tcagcctcgg ccgcgtccat
     5281 catgcgcccg gtcaagatga gatccatcgc ctttgccttt ccgatcgcgt gcgtcaggcg
     5341 ctgcgtgccg ccgatgccgg ggatgactcc gagcttgatc tccggctggc caaattttgc
     5401 ggtatcggcc gcgatcacga tatcgcactg catcgcgagc tcgcagccgc ctccgagcgc
     5461 gaagcccgcc actgccgcaa cgatcggctt gcgcgctttt gccgcagcat tccagtcgga
     5521 gacgaaatcg cccaggtagg cctcgatgaa cggcttgtcc gccatttcct tgatatcggc
     5581 cccggcggcg aaggctttgt cgctgccggt gatgagcata cagccgatct tgtcgtccgc
     5641 atcgaaaccc tcgatcgcct tcgttaactc ggcgatcaac gctttgttga gggcgtttag
     5701 cgcgtgaggg cggttaaggc ggatgattcc aacacggccc ttcgtttcga cgacgatatt
     5761 ctcgtaggtc atggctgatc ctgatttaac cgagattgtt cagtcgagct tggcgcccga
     5821 acgtttcacc acgtccgccc acttggcgga atcggccttg attgttgccg caaattcttc
     5881 gggcgtgccg ccgccaggct cgtcgccgaa tttagagaat ttttcgagga aagcctccga
     5941 tttgagtacc ctgttaatcg ctgcgttcag cttgtcgatg attgggcgcg gcacggccgc
     6001 cggcgcgatg actcctgtcc aggttgcggc aacgtatcca ggcacgcccg cttccgcgac
     6061 agtcggcaaa tccggaaatg atggcgagcg atggtcgccg gtgaccgcca gcgcccggac
     6121 cttgccgtca cggccaaaag gcgcgatgga ttgcaaactt tcaaacatca aatcgacatg
     6181 ccccgcgatg agatcattga ttgccagtga gccgccgcga tagggcacgt gaacgatttt
     6241 ggttccggtc atgtgcttta aaagctctgc accaacatgg cccggcgaac cgttgctgga
     6301 cgaagcattg gtgagtttcc ccggattctg cctggcgtaa tcgatcaact ccttgaccga
     6361 attcacaggc agtttcggcg agaccgcaag taacatttgc ccgcgcgtga caagggcaat
     6421 cggctggaag tcgcgctcaa tgtcgtacgg aagctttgca accatatggc gcgtgatcgc
     6481 gagcgcgccg atcggcccca tgccgatggt gtagccgtcc ggcggagact tcgcgaccag
     6541 atcgagcccg atggtcagcg cgcccccggg acggttctcg acaatgatct gttgtcccag
     6601 ctcctgggtc agcaccgccc cgagaagtcg cgcagcgttg tcggtggcac tgccgggtgc
     6661 ctgcggcacg atcagacgaa ctggatggtc cggataatcc gccgccgacg cggtgaccag
     6721 cgtcgccgca agcgtcagtg caatcatcaa gaccttgcgc atgtttttga aaccccgcgt
     6781 gggcgcagcc atggctcatt tctggcacga cgggcaatag aaggtcgagc gcccggattg
     6841 cacgatacgc ttgatccggc ctttgcagcc cgggcacggc gcgccctcgc gatcgtagac
     6901 cctgaaattg tgctggaaat cgccaagcga gccgtcggcg cgccgatgat cccgcagcga
     6961 ggatcccccg gcttcaatcg catccatgag caccgccttg atggcctcaa ccagattttg
     7021 agcgcgctca ttcggtctgc cattgcgatc ggcgagggtc gaagcctgcc gcttgggcga
     7081 gagccgtgcg cggaataagg cttcgcaaac ataaatgttg ccgagaccgg ccacgacacg
     7141 ctggtcgctg agtgccgctt tcagcgacgt cctcttgccg acgcacgctt tcgccagcat
     7201 cgctgcatcg aatgcatttc ccagtggctc aggcccgagc gagcgcagca atggctcctg
     7261 ctcaagcctg gcgcgagcca cgagcttcat cgagccgaag cgccgcggat cgttgaacgt
     7321 cactgttacg ccattcgaca tgtggaagat gatgtgatcg tgcgcggtgc tcctggatcg
     7381 ttcgtgatgg tactttccag gcgtgctgtt cttgccgacg cgaaacgatc cggacatgcc
     7441 gagatgcatc agcaacacgt caccggacga aagatcggcg agcagatatt ttgcgcggcg
     7501 ccccaatccc tttacgagct tgccgtgcaa cttttccgca aagccttttg gcaacggcca
     7561 acggagatcg ccacgccgca cttcgacctt gtcgaagcgc tcgccttcca tggctggcgc
     7621 caggccgcgc cgcacggtct cgacctccgg taattcgggc atgattaacc gctcccgcgc
     7681 tgacacatag cgccctcgga gcccccgcgc tatggtcttg cccgcaaagg actatcggtc
     7741 atgcctacag acggcgagca gacccatttc ggctaccgga gcgtgcctct cgacggcaag
     7801 cagcggcggg tcgatgacgt ttttcactcg gtcgcccgtc gctacgatct gatgaacgac
     7861 ctgatgtcgg gtggtctgca tcgcgcctgg aaggacgcgc tggtgacggc aatcaatcca
     7921 cccaggagcg agcggaactt ccgcgcgctc gatctcgccg gtggcaccgg cgacgttgcg
     7981 tttcgcgtca ttgaggcagg cggccacggt acccgggtca ccgtgtgcga tatcaacccc
     8041 gacatgctcg aggtgggacg cgaacgcgcc gcggcgcgcg gaattgaccg cgtggtgttc
     8101 gagcaaggca atgccgagcg gctggcctat cccgatcgga gcttcgattg cgtcaccatc
     8161 gccttcggca tccgtaatgt gccgcgcatc gagcatgcgc tcgaggaggc acaccgcata
     8221 ctcaggatcg gcgggcgatt tctctgcctc gagttttcct cggtcgatgt acccggcatc
     8281 gataccttgt atgagctcta ttctttccag gtcatcccgc gcttggggga aatcgtgacg
     8341 ggagaccacg acgcctatca atacctcgtc gaatcgatcc gtaagtttcc caagccgaag
     8401 gtatttgcgc ggatgctcga aactgcaggc ttccgccggg tttcgttcac gccgatgacc
     8461 ggcggcgttg tggggctgca ctcgggctgg cggttgtgat caccgctttt tctcacctgc
     8521 ttcgtttggc gcgcgccggt ttcgtgtttg cgcgcgaggg cgtctttgcg ctggttgata
     8581 cgcggccgtt gccggtcccc gccaagaccg ccattgcgct tgcccggctg ctcgagcggc
     8641 cgtccgcaaa acacggcgaa aaccgcctgg ctgccgcatt gacccggctt gggccgacat
     8701 atgtcaaact cggccaattt ctggcgacgc gtccggatgt cgttggggcg gcaatcgcgc
     8761 gtgaccttga aagtctccag gacaagatgc cgccgtttcc gcaaagcgaa gccgaggcgg
     8821 cggtcgttgc cgccttcgac aagccgctgt ccgcgcttta cgcgagcttc ggatccgtgg
     8881 tcgccgccgc atcgatcgcg caggtccacc gcgcagaggt tgaggcaaac ggggagcgca
     8941 aggcggttgc ggtcaagatc cttcgtcctg gcatcgaacg ccgcttcaag tctgatctcg
     9001 atgcattctt ctttgcggcg cgcaacgctg aagctttctc agcggaggcg cgccgcctac
     9061 gtctgatcga agtcgtgtcc acattggcgc gctcagtcgc gatcgagatg gattttcgtc
     9121 tggaggcagc tgccgcttcc gaaatggcgg cgaatacccg cgaagacgtt gactttcgcg
     9181 tgcccgccgt cgattgggac cggaccgccc gcgaggtgat gacactagag tggatcgacg
     9241 gaattcgtct cagcgaccat ggctcactgc gggccagggg gttcgatctg ccggaactcg
     9301 gccgcaacgt gatccagagc tttctgcgcc atgcgctgcg cgacgggttc tttcacgccg
     9361 acatgcatcc cggcaattta ttcgtggacg atgacggcaa actggtcgcg gtcgacttcg
     9421 gcattatggg tcggctgggt ccgaaggagc gccgtttcct tgccgaaatc ctgcttggct
     9481 ttatcaccag ggactatcgc cgcacggcgc aggtacattt tgatgcggga tatgtaccaa
     9541 ggcaccattc cgtcgaaagt tttgcgcaag ccattcgtgc gatcggcgaa cccattcaca
     9601 accgcacggc ggaagacatc tcgatggcga agctgctgat gctgctgttc gaggtgaccg
     9661 gcttgttcga catgcgcacg cggccggagc tgctgctgct gcaaaagacc atggtggttg
     9721 tagagggcgt cgcccgctcg ctcgatccca agctcgacat gtggggcacc gccgatccgg
     9781 tcgtgcgcga atggatcgcg cgcaatctcg gaccggtggg acgactggaa gatgccgtcg
     9841 agggcgccgg cgaaatcggt cgatttttcg gcgaggttcc cggcttgctg acccgcgccg
     9901 ggacgctggt cgatcagttc gacgacatca cacgggatgg gctggtgctt tcgcccgaaa
     9961 ccgtatcggc aattggcgcg gcggaggcac ggcgcaaccg ctggaccgcg atcgcactat
    10021 gggtgattgc gatcctgctg gccttcctta tctggcggct gatctgagaa acagctctgc
    10081 caacttgtcg atgctctgat tccagacggc ctccatgcct gcgagcattt gcagagcgat
    10141 cggaacgagg ccgatggcgt aactctgcag cgtcaggttg tccttccatc gctctcgctg
    10201 agaatgacgg ttgtcttccc ttcgagcagg tgcttgccgt ccttgtcgat ggcaatattc
    10261 ctgaacacaa gcctccccgg tggttcaagt tcgctaaact cggcctttca tcgggtagtc
    10321 gttgccgtcg ggcccgcgca tcacgatgta caggctgccg ccggcagttg cggtcattct
    10381 tgcttaaccc ttataggcac gctcgatctt gcttaagcct tatcggcacg ctcgatcgct
    10441 gcgaggtcgg agagcttcat tttgtttcct ttcgtactga ctaagcgctg accatcgttt
    10501 tgaagccgcc gaagatcatg cgcttgccgt cgaacggtat ttttttcgat ttcatcatcg
    10561 cgtcgatacg tttgtctttc atgaccttcg tcatgacgcg atcgcggtct ccgcgcgacc
    10621 ggtagacaat gtaagaaaac actacgatct cgccccgctt gagcttgacg ctgcgcggaa
    10681 acgacgtgcg ttttccgtgc ggaacgtcgt cggcgacaca ttcgtggaat tccagggcgc
    10741 cgtgctggcg ccagaccttg ccagcctgct tggccatgcg gcggtaggtg gccaactgcc
    10801 tcttcttcac cggaagtaca aagccgtcga cataggtcat cgatccgctc ctctctcatg
    10861 actggcaatt tgggggctgc aggccgtatt gataggtacc acccggccac agatccatct
    10921 ttgacgtgat cgccgacgca tcaaacgtgc gcgatatctc aaaatcctcg actgttgcgt
    10981 cgtcccttcc gaacgccgcg ctcacttcgg gtgaacgaga atcatccagt tcacgccgaa
    11041 tttgtcggcc aacatgccga agcgtggcga aaagaacgtc ttggtcagcg gcatcgtgac
    11101 ctttccgccg gacgacaggg cattgaacat gcgatcggcg tcggcctcgt ctctggcatc
    11161 gagcgaaagc gcgaaacctt tgaaatccgg attgcccttg ttttctccat ctgatgccat
    11221 gacccgcgcg tcaccgatct tcagagaggc atgcatcacc ttgttct
//
LOCUS       DASSRL010000021         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_315329, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000021 DASSRL010000000
VERSION     DASSRL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315329"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VFL49_00420"
     CDS             <1..1440
                     /locus_tag="VFL49_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7910471.1"
                     /translation="AYTDHEIGRVIQAVEDMDKLDNTLVIYINGDNGTSAEGGPLGTP
                     NEVAFFNGVTVPVADQLKWYEVWGTEQTYNHMSAGWSWMFDTPFSWFKQNASQLGGIN
                     QNMVVSWPARIKDKGGLREQFVHVIDVVPTILEAAGIRAPEIVDGIRQKPIEGTSFAY
                     TFDAKNAKAPSRHKTQYFEMMGQWALYHEGWFLSTKVNRAPWEAFGPANPDPLNNQVL
                     ELFDLNKDFSQSENVAAKYPQKAKEMKEMFIAEAKKFQVFPMDASVAARIVAPRPNIT
                     AGRTEFVYTRPMTGLPQGDSPLLLNTSYTVAADIEVPPGGAEGMILTSGGRFAGYGFY
                     LLKGKPVWLWNMVDLERLKWEGPDVLSPGRHTVEFDFKYDGLGAGTLAFNNFSGVGRP
                     GTGTLKVDGKVVATKTMEKTLPMILQWDESFDIGSDTLTGVNDADYKPPFTFTGKINK
                     ITLTIDRPKLSPEDIAKLQQAQRNNKVSE"
     gene            1546..1974
                     /locus_tag="VFL49_00425"
     CDS             1546..1974
                     /locus_tag="VFL49_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HET7910472.1"
                     /translation="MPTLNQLQLVISQAAAPAFILGAVASFLSVLLSHMGRIIDRSRA
                     IHDLPENHPRKKKLDASLPQLRLRARILHRAIYWAVGSGVATCLLMIVGFASAYFGIR
                     HEPGAALLFTVALGLFTAALVSFAREIGIALNRPEADWGE"
     gene            2031..>2800
                     /locus_tag="VFL49_00430"
     CDS             2031..>2800
                     /locus_tag="VFL49_00430"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7910473.1"
                     /translation="MPAAILLAAGLLIFATLPPRTVTMATGAPGGANHELGLRYQEVL
                     ARSGIRLRLVNTTGGLDNLARLRDRRSGVQIGFIQGGTTTKQESPQVESLGTIFFEPL
                     WLFYRSEIGHDIQAFRGRRVSIGPEGSAGRALALEILKRTKIDAVVGELLSFPPQEAA
                     DKLIAGDIDIAFIVSGWDSPAVRQLIEAEKIALVSVPRPDAFIALYPFLNKVVLPAGV
                     ADLATNRPPADVTLLAPKASLAVRSDLSSAIQYLLLNAA"
BASE COUNT          544 a          847 c          862 g          547 t
ORIGIN      
        1 gcctacacgg accacgagat cgggcgggtg atccaagccg tcgaggacat ggacaagctg
       61 gacaatactc ttgtcatcta catcaacggc gacaacggga ccagcgccga aggcggtccg
      121 ctcggcacac ccaatgaagt cgcgttcttc aacggcgtca ccgtccctgt tgcggaccag
      181 ttgaagtggt atgaagtctg gggcacggag cagacctaca accacatgtc ggccggctgg
      241 tcatggatgt tcgacacgcc gttctcgtgg ttcaagcaga acgcgtcgca gctcggcggc
      301 atcaatcaga acatggtggt gtcgtggccg gcacgcatca aggacaaggg cggcttgcgc
      361 gagcagttcg tgcacgtcat tgacgtcgtg ccgacgatcc tcgaagccgc aggcattcgt
      421 gcgcctgaaa tcgtggacgg catcaggcag aagccgatcg agggcacaag cttcgcctat
      481 accttcgacg cgaagaatgc caaggcgccg tcgcgccaca agacccagta tttcgagatg
      541 atgggccagt gggcactgta tcacgaaggc tggttcctca gcaccaaggt gaatcgggcg
      601 ccatgggagg cgttcggccc ggcaaatccc gatccgctca acaatcaggt gctcgagctc
      661 ttcgacctga acaaggactt cagccagtcc gagaacgtcg cggcgaagta cccgcagaag
      721 gccaaggaga tgaaagagat gttcatcgcc gaggcgaaga agttccaggt cttcccgatg
      781 gacgcgtcgg ttgcggcacg catcgttgcg ccgcgcccga acatcaccgc cgggcgcacg
      841 gagttcgtct acacccggcc gatgacgggg ctgccgcagg gcgactcgcc gctgctgctc
      901 aacacctcct acaccgtcgc ggccgacatc gaggtgccac cgggcggcgc cgagggcatg
      961 atcctcacgt ctggcggtcg tttcgccggc tacggcttct acctgctgaa gggcaaaccg
     1021 gtgtggctgt ggaacatggt cgatcttgag cgcctgaaat gggaaggccc cgacgtgctc
     1081 tcacccggca ggcacaccgt cgagttcgac ttcaagtacg acggcctggg cgcgggtacg
     1141 ctcgctttca acaatttctc cggtgttggc cgccccggca ccggcacgct gaaggttgac
     1201 ggcaaggtcg ttgccaccaa gacgatggaa aaaaccttgc cgatgatcct gcagtgggac
     1261 gagagcttcg acatcggctc cgataccctg accggtgtca atgatgccga ctacaagccg
     1321 ccctttacct tcaccggcaa gatcaacaag atcacgctga cgatcgaccg gccgaaactc
     1381 tcgccggaag acattgcgaa gctccagcag gcgcagcgca ataacaaggt cagtgaataa
     1441 acgcggtcag aatgggcttg atggccacgc cagccgaaac cctcggaaca tcttccggag
     1501 ggtttcctgg ttttgtcaac gcgtgaaacc ctgcggagga ttgcgatgcc aacactgaac
     1561 cagctgcagc ttgtgatatc gcaggcggca gcgcctgcat tcattcttgg cgcggtggcg
     1621 tcatttcttt cggtcttgct gtcccacatg gggcgcatca tcgacaggtc acgtgcgatc
     1681 cacgatctcc cggagaacca tcccaggaag aagaagctcg acgccagttt gccccaactg
     1741 cggctgcgcg cacgcatctt gcaccgtgcc atttactggg cggtcggcag cggcgtcgca
     1801 acctgcctgc tgatgattgt cggttttgcg agcgcctatt ttggtatcag gcacgagccc
     1861 ggcgctgctt tgcttttcac agtggcgctc gggctgttta ccgcggctct ggtttctttt
     1921 gcccgcgaga tcggcatagc tttaaaccgt ccggaagcgg actggggtga atgagttcgc
     1981 gttgatcgga gaatagcctg gaaagattct ggcactggat ttttttcgcg gtgcccgccg
     2041 ccattctgct ggcggcgggg ttgcttattt ttgcgacctt gcccccgcgc actgtgacca
     2101 tggcgaccgg cgctccgggc ggcgccaatc atgagctggg tctgcgctat caggaagtcc
     2161 tggcgagatc cggcatccgg ctgcggctgg tcaatacgac gggcggactg gacaatctcg
     2221 cgcgcctgcg cgatcgccgg tcaggcgtcc aaatcggatt tatccagggc ggcacgacga
     2281 cgaaacagga atcgccgcaa gtcgagtcgc ttggcaccat atttttcgag ccgctatggc
     2341 tgttttatcg gagcgagatc ggtcatgaca tccaggcctt ccgcggccgc cgcgtttcga
     2401 ttggcccgga aggaagcgcc ggccgcgcgc ttgcgctcga gatcctgaag cggacaaaga
     2461 tcgacgctgt tgtcggtgag ttgctgagtt tcccgcccca ggaggcggcg gacaagctga
     2521 tcgcgggcga tatcgacatc gctttcatcg tctcgggctg ggattcgccg gcggtgcgac
     2581 aactcataga agcggagaag atcgcgcttg tgagcgttcc gcggcccgac gccttcatcg
     2641 cgctttatcc gttcttgaac aaggtcgtgc taccggccgg cgtcgccgat ctcgcgacga
     2701 atcggccgcc tgcggacgtc accttgctgg ctcccaaggc gagcctggct gtgcgcagtg
     2761 atctgtcttc ggctatccaa tatcttctgc tcaatgccgc
//
LOCUS       DASSRL010000022         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_980331, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000022 DASSRL010000000
VERSION     DASSRL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980331"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFL49_00435"
     CDS             complement(<1..449)
                     /locus_tag="VFL49_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET7910474.1"
                     /translation="MIDVSLGKIAGVKLIRPAAQHDFRGTFVKTMHAEIFSKHGIPTQ
                     YAEQYYSVSKKNVLRGLHFQTPPHEHYKLVTCIEGEAFDVVVDLRNGSPSYGQHESFE
                     LNGAGGDSVFVPAGCAHGFYVRSETATLLYNVSSVHAPSHEAGIRWDS"
     gene            complement(446..1789)
                     /gene="rfbH"
                     /locus_tag="VFL49_00440"
     CDS             complement(446..1789)
                     /gene="rfbH"
                     /locus_tag="VFL49_00440"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HET7910475.1"
                     /translation="MLTRPKQVQQSDSLREQILSLGEQFFAEREADKAFIPGQTYIPC
                     SGKVLDARDLRNLLDASLDLWLTAGRYAGEFEAALAAQFGLAHCRLTVSGSAANLLAF
                     SALTSWKLHERRIAEGSEVITVAAGFPTTVAPIIQNRCVPVFVDVDLETANVNVDRLA
                     AAVGPKTRAIMLAHTLGNPFNLAAVTAIAKQHKLYVIEDCCDALGATYGGKPVGTFGD
                     LATISFYPAHHITTGEGGAVLTNRKSFATLVESYRDWGRDCWCPPAEANTCGKRFEWR
                     IRGMPEGYDHKYTYAHIGYNMKATDMQAAVGISQLQKADGFIAARRENFARLTATFRA
                     QDLDEHFILPQATPDSDPSWFGFLLTIRDGSPLKRREVVTYLENNKVGTRLLFGGNLT
                     RQPAFRNVEYRVAGNLANTDKLMNDAFWIGVWPGIGEAERSYIVSIFAGMVRHFT"
     gene            1871..2710
                     /locus_tag="VFL49_00445"
     CDS             1871..2710
                     /locus_tag="VFL49_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7910476.1"
                     /translation="MAISAPLILLTGATGFVGRHVLRALGKRGCRVCAVVRSGKQEEI
                     ARSKATEGIVASPDIFAENASWWADACRGVDTVIHAAWYAEPGQYLQSPKNQECLAGT
                     LRLAEGAVQAKVRRFVGIGTCFEYDPGDGRLSIKTPLKPSTLYARAKVDAFNALSASL
                     PRSGITFAWCRLFYLYGEGEDARRLVPYVRARLQGGEPAELSSGSQIRDFLDVRDAAR
                     MIVDVALGPEEGPVNICSGEPVTVREMAERIADEFGRRDLLRFGARAENPLDPPCVVG
                     IRG"
     gene            2786..3865
                     /locus_tag="VFL49_00450"
     CDS             2786..3865
                     /locus_tag="VFL49_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HET7910477.1"
                     /translation="MKARLEPRINLVGRTPLETVIPLAAPFIVFVDPASSCNFKCTFC
                     PTGHRDMIAETGRFQGVMKFEVFQKIIDDFSEFEKPIKVLRMYKDGEPFLNKRIADMV
                     AYAKRSGQVDYIDTTTNGTFLSPDRIGPVLEAGIDKINISVDGMNEETYFRFTGFRFD
                     FKKFVDNIKWIYANRGNCEIVVKIPAELITEGQRQEFFDTFGDHCDRIFVENFAPCWP
                     EFDIEKHTGVTISKGIYQQDIGETDTCPYIFYGYSVNSDGLVSSCFLDWGRKLVIGDV
                     RTQSMKAIWNSQAMNALRLQHLEGRRKQNQVCGNCGQLSHCLPDSIDAHRPMLLEKFK
                     SRVAVNPDLEPPGGSNRRFAAVAAE"
     gene            3862..>4140
                     /locus_tag="VFL49_00455"
     CDS             3862..>4140
                     /locus_tag="VFL49_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910478.1"
                     /translation="MRILHVTPHLGGGVGKAHAALGAVLPEIVQQTFVLLEAPRERRY
                     IDQIEAAGARVLTADNLSHVAALARDSDIVQFEFWNHPRMFECLARADF"
BASE COUNT          777 a         1245 c         1293 g          825 t
ORIGIN      
        1 gaatcccagc gaatgcccgc ctcgtgagag ggtgcgtgaa ctgagctgac attgtagagc
       61 agcgtcgcag tttcagatcg gacgtaaaaa ccgtgcgcgc atccggctgg aacgaagaca
      121 ctgtcgccgc ccgctccatt caattcgaaa ctttcatgct gcccgtaaga cggcgagccg
      181 ttccgcaggt cgacgacgac gtcgaaagcc tcgccttcga tgcaggtcac cagcttgtaa
      241 tgctcgtgcg gcggcgtttg aaaatggagg ccgcgcagga cgtttttctt cgacacggaa
      301 tagtattgct ccgcgtactg cgtggggatt ccatgctttg agaaaatctc cgcatgcatg
      361 gtcttcacaa atgtgccgcg gaaatcgtgc tgcgccgccg gtcggatcaa tttgacgccc
      421 gcgatctttc cgaggctgac gtcgatcatg tgaagtgccg taccatgccg gcgaagatcg
      481 acacgatgta gctgcgttcc gcctctccga ttccgggcca tacaccgatc cagaacgcat
      541 cgttcatcag cttatcggtg ttggcgaggt tgcccgcgac gcggtactcg acgttgcgaa
      601 acgccggttg gcgcgtcagg tttcctccga acagcagccg cgtgccgact ttattgtttt
      661 caaggtacgt cacgacctcg cggcgcttga gcgggctgcc gtcgcgaatg gtgagcagga
      721 agccgaacca gctcggatcg gaatccggcg ttgcctgcgg caggataaag tgctcgtcga
      781 ggtcctgtgc acggaacgtc gccgtcagcc tggcgaaatt ctcgcgacgg gcggcgatga
      841 agccatcggc cttctgcagc tggctgatcc cgaccgccgc ctgcatgtcg gtggccttca
      901 tgttgtagcc gatgtgggca taggtgtact tgtggtcgta gccttccggc attccgcgga
      961 tgcgccattc gaatctcttg ccgcaggtgt ttgcttcggc cggcggacac cagcagtcgc
     1021 gaccccagtc gcgataggat tcaacaagcg tggcgaagga tttccggttt gtcagcaccg
     1081 cgccgccttc gccggtcgtg atgtggtgcg ctggatagaa gctgatggtg gcgaggtcgc
     1141 cgaatgtgcc gacgggcttg ccgccgtagg tcgctcccag tgcgtcgcag cagtcctcga
     1201 taacgtaaag cttgtgctgc ttcgcaatcg cggtgaccgc ggcgaggttg aacggattcc
     1261 ccaaggtatg cgcgagcatg attgcgcgcg tcttcggtcc gaccgccgcc gccaggcgat
     1321 cgacattcac attggctgtc tccagatcaa cgtcgacaaa aacggggacg cagcggttct
     1381 ggataatggg cgcgaccgtg gtcggaaatc cggcggcgac agtgatgact tcgctgcctt
     1441 ccgcaatcct tcgctcgtgc agcttccacg atgtcagcgc cgaaaatgcc agaaggttgg
     1501 cggccgaacc cgagaccgtg agtcggcagt gcgccaggcc aaattgtgcg gcaagcgccg
     1561 cttcaaactc accggcgtaa cggcctgcgg taagccatag gtcgagcgat gcgtcgagaa
     1621 ggttgcgcag gtcgcgtgca tcgagaacct tgccggagca ggggatgtag gtctgtccgg
     1681 gaatgaaagc cttgtcggcc tcgcgctcgg cgaagaattg ttctccaagc gacagaatct
     1741 gctcgcgcag cgagtctgat tgctggactt gcttcggcct cgtcagcatc gggcggtagt
     1801 ctcgacgcgc cgcgacagct tggcgcgccc gaatcacata ccatcaggat gcccgattcg
     1861 cgatcacgtc atggccatct ctgctccatt gattttgttg accggcgcta ccggatttgt
     1921 cggtcggcac gtgctgcgcg ctcttggcaa gcggggctgc cgcgtgtgcg cggtcgtgcg
     1981 cagcggcaag caggaggaga tcgcgcgcag taaggccact gaagggattg tcgccagtcc
     2041 tgatattttc gcggagaatg cgtcgtggtg ggcggacgcg tgccgtggcg tcgacacggt
     2101 gatccacgcg gcctggtacg ccgaacccgg ccagtatctg cagtcgccga aaaaccagga
     2161 atgtctggcc ggtacgctgc ggctcgccga gggggcggta caggccaagg tgcgccgctt
     2221 tgtcgggatc ggcacatgct ttgaatacga tcccggcgat ggccggttga gcatcaagac
     2281 gccgctcaag ccatcgacgc tctatgcgcg cgccaaggtc gacgccttca atgcgctttc
     2341 ggcgtcgctg ccccggagcg gcattacgtt cgcctggtgc cggctcttct atctctacgg
     2401 cgaaggcgag gacgcccgcc ggctcgtgcc ctatgtgcgg gccaggctgc aaggcggcga
     2461 gccggcggag ttgtcgagcg gcagccagat ccgcgacttt ctcgatgtgc gtgacgccgc
     2521 gcgcatgatc gtggatgttg cgctcgggcc ggaggagggg ccggtcaaca tctgctccgg
     2581 cgaaccggtc accgttcgcg agatggccga gcgcatcgcc gacgagtttg gccgccgcga
     2641 tctgctccgc tttggcgccc gcgccgagaa tccactcgat ccgccctgcg tcgtcgggat
     2701 ccgcgggtga cgagcttacg tttggttaac tactgttaac tattctcgga caggaacgat
     2761 cgcgctctct actgcaggat atcccatgaa ggcccggctc gaaccgcgaa tcaatctcgt
     2821 cggacgcacg ccactcgaaa ccgtcatccc gcttgccgcc ccgttcatcg tcttcgtcga
     2881 cccggccagc tcctgcaact tcaaatgcac gttctgcccg accgggcacc gcgacatgat
     2941 cgcagagacc ggccgcttcc agggcgtgat gaagttcgag gtctttcaga agatcatcga
     3001 cgatttcagc gagttcgaga agccaatcaa ggtgctgcgc atgtacaagg acggcgagcc
     3061 gttcctgaac aagcgcatcg ccgatatggt tgcctacgca aagaggtcgg gtcaggtcga
     3121 ttacatcgac acgacgacga atggcacgtt tctttcaccc gaccggatcg ggcccgtact
     3181 tgaggccggc atcgacaaga tcaatatctc cgtcgacggc atgaacgagg aaacctattt
     3241 ccgcttcacc ggcttcaggt tcgacttcaa gaagttcgtc gacaacatta aatggatcta
     3301 cgcgaacagg ggcaattgcg agatcgttgt caagatccct gccgagctca ttaccgaagg
     3361 gcaacgtcag gaattcttcg acaccttcgg cgatcactgc gaccgcatct ttgtcgagaa
     3421 cttcgcgccg tgctggccgg aattcgacat cgaaaagcac accggcgtga cgatcagcaa
     3481 gggcatctac cagcaggaca tcggcgaaac tgatacgtgc ccctacatct tctacggcta
     3541 ctcggttaat tccgacgggc tggtcagctc gtgcttcctc gactggggac gcaagctggt
     3601 gatcggcgac gtgcgcaccc agtcgatgaa ggcaatctgg aattcacaag cgatgaacgc
     3661 gctgcggctg cagcatctcg agggtcggcg caaacagaac caggtttgcg gcaattgcgg
     3721 ccagttgagc cactgcttgc cggacagcat cgacgcgcat cgtccgatgt tgctggagaa
     3781 gttcaagtca cgcgtcgccg ttaatcccga tctcgagccg ccgggcggca gcaatcgccg
     3841 gtttgctgcg gtggcggccg aatgagaatc ctgcacgtca cgccgcatct gggcggcggt
     3901 gtcggcaaag cgcacgcagc tctcggcgcg gtcctgcccg aaattgtgca gcagacgttt
     3961 gtcctgctcg aggcgccgcg cgagcgccga tacatcgatc agatcgaggc ggccggcgcc
     4021 cgcgtgctga cggccgacaa tctctcgcac gtcgccgcgc tggcgcgcga ctccgatatc
     4081 gtgcagttcg aattctggaa tcatcctcga atgttcgagt gcctggcgcg ggcggacttt
//
LOCUS       DASSRL010000023         4491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1295393, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000023 DASSRL010000000
VERSION     DASSRL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295393"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFL49_00460"
     CDS             <1..946
                     /locus_tag="VFL49_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET7910479.1"
                     /translation="ISLGMKQLLDDPWQGIEGKYPVGARFKGRVTNITDYGAFVELEP
                     GIEGLIHVSEMSWTKKNVHPGKIVSTSQEVEVQILEVDPVKRRISLGLKQTMRNPWEV
                     FVEKHPPGSTVEGEVKNKTEFGLFLGLDGDVDGMVHLSDLDWKRPGEQVIEEYKKGDI
                     VKAQVLDVDVEKERISLGVKQLAGDPMESAGDLKKGSVVTCEVTEVKESGIDVKIVGT
                     DLTAFIKRADLARERGEQRPERFSVGQKVDARVTQFDRKTHKVGVSIKALEVAEEKEA
                     IAQYGTADSGASLGDILGAALKQRAGDKSDVEAEKAEE"
     gene            1141..2115
                     /gene="sppA"
                     /locus_tag="VFL49_00465"
     CDS             1141..2115
                     /gene="sppA"
                     /locus_tag="VFL49_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7910480.1"
                     /translation="MSLDADAIVDRRRMRRKLTFWRVTAVLVALLAIGGGALLLVPGS
                     RLMAPGAYISRIKIQGLIRGNQDRVEALDRLGRSRASAVIVHIDSPGGTTAGSEQLYD
                     ALRVLQSKKPMVVVVDGLAASGAYIAALSADHIVANETSLVGSIGVLFQYPNFTEVLK
                     TIGIKVEEVKSSPLKAAPNGFEPTSPEARAAIEAIVLDSYAWFKDLVKSRRKMDDGQL
                     TRVADGRVFTGRQAVGLKLIDGLGNEKTALAWLEKEKKVPANTPVRDYSLQPRFSELS
                     FLHVAAWTFEAVGLSAVAHRIEESGAVQAVERLNLDGLLALWHPPSTN"
     gene            2144..2434
                     /gene="ihfB"
                     /locus_tag="VFL49_00470"
     CDS             2144..2434
                     /gene="ihfB"
                     /locus_tag="VFL49_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET7910481.1"
                     /translation="MIKSELVQRIASQNPHLYQRDVENIVNAILGEITSAMAKGDRVE
                     LRGFGAFSVKHRPARTGRNPRTGAHVAVDKKSVPFFKTGKEMRERLNKPGTV"
     gene            2519..2884
                     /locus_tag="VFL49_00475"
     CDS             2519..2884
                     /locus_tag="VFL49_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HET7910482.1"
                     /translation="MFRKIVSAVILVPLAAVIILFAVANRQAVTVSFDPFSTASPAYA
                     VTLPLFVLVVMLIILGVLIGGTAAWAGQARWRRAARRLDADVRALHQELDVIRRRFAP
                     ETSRPPDPSPYTVIPPPLP"
     gene            2936..3640
                     /locus_tag="VFL49_00480"
     CDS             2936..3640
                     /locus_tag="VFL49_00480"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7910483.1"
                     /translation="MPLSIKICGLKNPQALDVALESGANLVGFVFFPPSPRNIGLEAA
                     RALGQRVQGRAAKVALTVDANDETLLDIVNALNPDMLQLHGSETPERVVAVRTRFGLP
                     VMKALAIAERRDLSPVREYAQFADRLIFDARPPKDATRPGGLGRAFDWALLKNINAGV
                     PFMLSGGLDSDNVAEALRITRAPGLDVSSGVERSPGEKDPEKIRAFIRAARAAEAEVF
                     SPPLQLGEASIVRPVK"
     gene            3649..>4491
                     /gene="trpB"
                     /locus_tag="VFL49_00485"
     CDS             3649..>4491
                     /gene="trpB"
                     /locus_tag="VFL49_00485"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7910484.1"
                     /translation="MTVQQPNSFRTGPDERGHFGIYGGRFVAETLMPLILELEVAYNK
                     AKADPKFQKEMDAHLATYVGRPSPLYFAERLTQHCGGAKIYFKREELNHTGAHKVNNV
                     LGQIMLAQRMGKGRIIAETGAGMHGVATATLCAKFGLPCIVYMGALDVERQKPNVLRM
                     KILGAEVRPVESGACTLKDAMNEALRDWVTNVSDTFYCIGTVAGPHPYPAMVRDFQSI
                     IGRETRDQLHAAEGRLPDSLIACIGGGSNAMGLFHPFLDERSVEIYGVEAAGLGLNSK
                     HAASI"
BASE COUNT          880 a         1337 c         1386 g          888 t
ORIGIN      
        1 catctcgctc ggtatgaagc aactgctcga cgatccgtgg caaggcatcg aaggcaagta
       61 tccggtaggc gcccgcttca aaggtcgcgt caccaacatc accgactacg gggcctttgt
      121 ggagcttgaa cccggcatcg agggtctgat ccacgtgtcc gagatgtcat ggacaaagaa
      181 gaacgtccat ccgggcaaga tcgtctccac ctcgcaggaa gtcgaggtgc agattctcga
      241 ggtcgatccg gtcaagcgcc gtatttcgct tggcctcaaa caaaccatgc gcaacccgtg
      301 ggaggtgttc gtcgagaaac atccgccggg ctcgacggtc gaaggtgaag tcaagaacaa
      361 gaccgaattc ggcctgttcc tcggccttga tggcgatgtg gacggaatgg ttcacctgtc
      421 cgatctcgac tggaagcgtc ccggcgagca ggtgatcgag gagtacaaga aaggcgacat
      481 cgtcaaggcg caggttctgg atgtcgacgt cgagaaggag cgcatttcgc tcggcgtcaa
      541 gcagctcgcc ggcgatccga tggaatcagc aggcgacctc aagaagggat cggtcgtcac
      601 ctgcgaggtt actgaggtga aagaatccgg gatcgacgtc aagatcgtcg gcaccgatct
      661 cactgccttc atcaagcgtg ccgatctcgc ccgcgagcgc ggcgagcaac gtccggagcg
      721 cttctcggtc ggccagaaag tggacgcccg cgtcactcag ttcgaccgca agacccacaa
      781 ggtcggcgtt tccatcaagg cgctggaagt ggccgaggag aaagaggcaa tcgcccagta
      841 cggtacagcc gattcaggcg cttccctggg cgatattctc ggtgcggctc tcaagcagcg
      901 ggccggcgac aagtccgacg tcgaagccga aaaagccgaa gaataggccg gttgcatcct
      961 tgaaagctga atgccccggg ctggtccccg gggtttccat ttgccatcgc ctaaactcgg
     1021 ccacatcgtt gcgcgatgcg cggcaatgac cggtacaatc tgattacgat tcgacctatg
     1081 tgatgtggag ccggtgcacg atttgctgca ctatcacgcg tgaacaaatg gagcgaagcc
     1141 atgtcgctcg atgccgatgc catcgttgat cgccgccgca tgcggcgcaa gcttacgttc
     1201 tggcgcgtaa ccgccgtcct ggtggcgctg cttgcaatcg gtggtggcgc gcttttgctc
     1261 gttcctggga gccgccttat ggcgccaggg gcgtatatct cgcgcatcaa gatccagggc
     1321 ctgatccgcg gtaaccaaga cagggtcgag gcacttgacc ggcttgggcg ctcgcgcgcc
     1381 agcgcggtga ttgttcacat cgacagcccc ggcggtacca cggctggctc cgaacagctt
     1441 tatgacgcgc ttcgcgtgct gcagagcaaa aagcctatgg tggtggtcgt tgacggcctt
     1501 gcggcatccg gagcctatat cgccgcgctt tcggccgacc acattgttgc gaatgaaacc
     1561 tcgctggtcg gctcgattgg tgtccttttt caatatccaa atttcaccga ggtgctgaaa
     1621 acgatcggta tcaaggtgga agaggtgaag tcgtcgcccc tgaaggcggc accgaacggc
     1681 ttcgagccta caagtccgga agcacgtgcc gcgattgagg cgattgtgct ggattcctat
     1741 gcctggttta aagatctcgt aaagagccgc cggaaaatgg acgacggcca attgacccgc
     1801 gtcgccgatg ggcgtgtctt taccggccgg caggcggtag ggctcaagct gatcgatggg
     1861 ctcggcaacg aaaagaccgc tttggcctgg ctggagaagg aaaaaaaggt gcccgccaat
     1921 acgcccgtgc gggactattc gttgcagcca cgcttcagcg agctttcttt tctgcacgtc
     1981 gctgcctgga ctttcgaagc ggttgggctt agcgccgttg cccaccgcat cgaagaatcg
     2041 ggcgcggtgc aggcggtcga gagacttaat cttgacggtc tcttggcgct ttggcaccct
     2101 ccgtccacca attgagtaac actcgaccgg cggggcggca tcaatgatca agtcagagct
     2161 tgtccagcga attgcctccc agaatcctca cctctaccaa cgcgacgtcg aaaatatcgt
     2221 caacgccatt cttggcgaga tcacgagcgc catggcgaaa ggcgaccgcg tcgagttgcg
     2281 aggctttggt gctttttcgg tgaagcaccg gccggcgcga accggccgga atccccggac
     2341 cggtgctcac gttgccgtcg acaagaaatc ggtgccgttc ttcaagaccg gcaaggaaat
     2401 gcgtgagcgg ttgaacaagc cgggtacggt ctagagacgg tttttccggt ttacggagat
     2461 gctatggtta cggccgggca acgtcccggc cgtcgatttt ttgactaggg cgtcgacaat
     2521 gtttcgcaaa attgtgtcgg cggtgattct cgtgccgttg gcggcggtca tcatcctctt
     2581 tgcggtggcc aaccggcaag cggtgacagt gtcgttcgat cccttctcaa cggcgagccc
     2641 ggcctatgcc gtgacgttgc cgctcttcgt gctcgtcgtg atgcttatca ttcttggcgt
     2701 gttgattggc ggcactgccg cctgggccgg ccaggcacgc tggcgccgcg ctgcccgtag
     2761 gctcgatgcc gacgtgcgtg ccctgcatca agagctcgat gtgatccgac ggcgctttgc
     2821 acccgaaacc tcgcgcccgc ccgacccgag cccgtacacg gtcattccgc cgccgcttcc
     2881 gtaacggacg ttccgctttg cggccggctg gataaggcgg tagaacgcgc tcataatgcc
     2941 tttgtcgatc aaaatctgcg gccttaagaa cccgcaagct cttgatgtgg cgctggaatc
     3001 cggggcgaac ctcgttggat tcgtgttctt tcccccgagc ccccgtaaca tcggcttaga
     3061 ggcggcgcgc gcgctcggcc agcgcgtgca aggccgtgcc gccaaggtcg cgctcacggt
     3121 cgatgcgaac gacgagacgt tgcttgacat cgtcaatgcg ctcaatcccg acatgctgca
     3181 gctgcacggc agcgaaacac ctgagcgtgt cgtcgccgtc cgtacgcgtt tcggtctgcc
     3241 ggtgatgaag gcgctggcga ttgccgagcg ccgggatctc tcgcccgtcc gcgagtatgc
     3301 gcagtttgcc gaccggctga ttttcgatgc gcggccaccg aaggacgcaa cccgcccggg
     3361 tggactggga cgggccttcg actgggcctt gcttaagaat atcaacgcgg gcgttccctt
     3421 catgctgtcg ggcggactcg attccgacaa tgtcgcggag gcgcttcgca tcacgcgtgc
     3481 gccgggcctc gatgtatcct cgggtgtcga acgatcgccg ggcgaaaagg acccggaaaa
     3541 aatccgggca ttcatccggg cggcccgcgc ggcggaagcc gaagtctttt cgccgcctct
     3601 ccagcttggc gaggcttcga tcgtccgtcc ggtcaaatag gctgcaccat gaccgtgcaa
     3661 caacccaatt cattccgtac cggccccgac gagcgcgggc atttcggcat ctacggaggt
     3721 cgtttcgttg ccgaaacgct gatgccgctg atccttgaac ttgaggtagc ctacaacaag
     3781 gccaaagccg atcccaaatt ccagaaggaa atggatgccc atctggcgac ctatgtcggc
     3841 cgcccgtcgc ccctttattt cgcggagcgc ctcacgcagc actgcggcgg agcaaaaatc
     3901 tatttcaagc gcgaagagct caaccatacc ggcgcgcaca aggtgaacaa cgtgcttggc
     3961 cagatcatgc tggcgcagcg catgggcaag ggacgcatca tcgccgagac gggtgccggc
     4021 atgcatggtg ttgcgacggc gaccttgtgc gccaaattcg gcttaccgtg catcgtctac
     4081 atgggggcgc tcgacgtcga gcgacagaaa ccgaacgtcc tcagaatgaa gatcctcgga
     4141 gcggaggtgc gtccggtcga atcgggtgcg tgcacgctca aggacgcaat gaatgaagcg
     4201 ttgcgcgact gggttaccaa tgtcagtgac acgttctatt gcatcgggac cgtcgccggc
     4261 ccacacccgt atccggcgat ggtgcgcgat ttccagtcga ttatagggcg cgaaacccgc
     4321 gatcagctac acgcagcaga gggccggctt cccgactcgc tcattgcctg catcggcggc
     4381 ggctcaaacg ctatgggtct gtttcacccg tttctcgatg agcggtcggt cgagatctac
     4441 ggcgtcgagg cggccggact tggactcaac agcaagcacg cggcctcgat t
//
LOCUS       DASSRL010000024         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_998061, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000024 DASSRL010000000
VERSION     DASSRL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998061"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="galE"
                     /locus_tag="VFL49_00490"
     CDS             <1..948
                     /gene="galE"
                     /locus_tag="VFL49_00490"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7910485.1"
                     /translation="HELVDAGEPVVVLDNLSSGFRFLIPTSVPFIAGSTGDRPLVGEL
                     IAHHRVTAIIHFAASIVVPDSVTDPLGYYSNNTMNTCALLDVAIKSGVREFIFSSTAA
                     VYGSAGDAPVREDAPTVPISPYGTSKLMSEIMLHDAAKAHGLRFVALRYFNVAGADPK
                     LRTGQATPAATHLIKIACETAIGRRPKMNVFGTDYPTPDGTCIRDYIHVSDLAKAHSA
                     ALAYLRSGGQSVTFNCGYGHGSSVLEVIAAVRRASGRDFPVEISGRRPGDPTSLVANV
                     DRIRSVLSWRPQFQNLDTIVSHALAWEKQLAARRKSAVA"
     gene            1076..>2156
                     /locus_tag="VFL49_00495"
     CDS             1076..>2156
                     /locus_tag="VFL49_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET7910486.1"
                     /translation="MNEQSLPAAEERYGALTLIRRLLAEQGLVHWRKYAVAFILMAVA
                     AGCTAFSAYLIGDVINQAYVNRNLPGIIVLGAITVLLFSLKGLATYGHSVLLSRIGNR
                     IVADNQRAVFSKLLNEGLGFFSDRHSTEFIARLSTGAAAATQVINLLITSVGRDLLSL
                     IGLVAVMVIQDPLMSFFSFVVAPPAFFVLRKLIRRIYSIARSQFHGGTRIIETLQETL
                     QGIRIVKAFTLEDIMRERFDQNVAAVEHEANKMARVSNRASPLMETLGGCAIAIAITY
                     GGYRVIETGATPGQFFSFITAFLLAYEPAKRLARLNIELNSALVGVRILFEVLDTPPS
                     EAPDGAKPDLRTTAGRLEFKDVHFAY"
BASE COUNT          399 a          665 c          611 g          481 t
ORIGIN      
        1 cacgaactcg tcgatgcggg cgagcctgtc gtcgtgctcg ataatctgtc gagcggcttc
       61 cggttcctga taccgacatc cgttcccttt atcgccggca gcacgggtga ccggcctctt
      121 gtcggcgaac ttatcgcgca ccatcgcgtc accgcgatca tccattttgc cgcatcgatc
      181 gtggtgccgg attccgtcac cgaccctctc ggctactaca gcaacaatac gatgaatacc
      241 tgcgcgcttc tcgacgtagc catcaagtcc ggcgtgcgcg aattcatttt ttcttcaacc
      301 gccgccgtct atggcagcgc gggagacgcg cccgtgcgcg aggatgctcc gaccgttccg
      361 atttcgcctt acggcacttc aaagctgatg tccgagatca tgcttcatga tgccgccaag
      421 gcgcatggtc ttcgtttcgt tgcgcttcgc tattttaatg tcgccggcgc cgaccccaaa
      481 ttacgcacgg gccaggcaac gccggcagca acgcatctga tcaagatcgc ctgcgagacc
      541 gcgatcggca ggcggccgaa aatgaacgtg ttcggcaccg actatccgac gcccgacggc
      601 acctgcatcc gcgactacat tcatgtcagc gatctcgcca aggcgcactc ggcggcgctt
      661 gcctatctgc gcagcggggg gcaaagcgtc acgtttaatt gcggctatgg ccacggctct
      721 tccgttctcg aggtgattgc agcggtacgg cgcgcaagcg gccgtgattt tccggtggaa
      781 atatccgggc gccgccccgg cgatccgacc tcgctcgtgg cgaatgtgga ccgcatccgt
      841 tcggtgctgt cctggcgtcc gcaattccag aatctggaca cgatcgtttc ccatgcgctc
      901 gcatgggaaa agcagctcgc ggcccgacga aagagcgcag tggcctgatt gggggccgca
      961 cagccgtttc ccgcgctttt taccaagttt ttggcttgaa aatttctttg ctgacaggca
     1021 aggaaacgct cattcctgcc cgacgtgtca tttttaacag cccgacagag ggcggatgaa
     1081 cgaacagagt ctgccagcgg ccgaagagcg ttacggcgcc ctgaccctga tccggcgttt
     1141 gctggctgaa cagggcttgg tgcactggcg caaatacgca gttgcattca ttttaatggc
     1201 ggtcgccgca ggctgtacgg ccttcagtgc gtatctgatc ggcgacgtca tcaatcaggc
     1261 atatgtcaat cggaacttgc cgggcatcat cgttctcggc gcgatcacgg tccttttgtt
     1321 ttcgttaaag ggcctcgcga cctacggcca tagtgtgctg ctctcgcgca tcggaaaccg
     1381 aatcgtcgcg gataatcagc gcgccgtatt ctcaaaactg ctgaacgaag gtcttggatt
     1441 tttttccgac cgtcattcga ctgaattcat cgcccgactg tcgaccggcg ctgccgccgc
     1501 aacgcaggtc atcaatctgc tgatcacttc ggtcggacgc gacctgctct cgttgatcgg
     1561 gctggtcgcg gtgatggtca ttcaggaccc cttgatgtcg ttcttcagct tcgtggtggc
     1621 gccgcctgca ttctttgttc ttcgcaaatt gatccgtcgc atctactcaa tcgcccgcag
     1681 ccagtttcat ggcggaacgc gcattatcga aacgctgcag gagacgcttc agggcattcg
     1741 catcgtaaaa gcctttacgc tcgaagacat catgcgtgag cgcttcgacc agaacgtcgc
     1801 ggcggtcgag catgaggcca acaagatggc gcgggtctcg aaccgggcca gccccttgat
     1861 ggagactctc ggcggctgcg caatcgcaat tgccatcaca tatggcggct atcgtgtgat
     1921 cgaaacgggc gccacccccg gccagttctt ttctttcatc accgcattct tgctggctta
     1981 cgagccggcc aagcggcttg ccaggctgaa cattgagctc aacagcgcgc tggtcggggt
     2041 gcgcattttg ttcgaggtgc ttgacacgcc gccgagcgag gcgcccgacg gcgccaagcc
     2101 tgacttgcgg acgaccgccg gacggcttga attcaaggac gtgcatttcg catatc
//
LOCUS       DASSRL010000025         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_70717, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000025 DASSRL010000000
VERSION     DASSRL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70717"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFL49_00500"
     CDS             complement(<1..766)
                     /locus_tag="VFL49_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910487.1"
                     /translation="MAELIVKDVALRLGDNEILKGVSLSVPAGEVVALLGPSGSGKTT
                     LLRAVAGLETPHQGSIAIGNTVFFDASREIDLPAEKRGLGLVFQSYALWPHRTVFDNV
                     AYGLKLRGIPAEEIKARVEKTLSQIGLGHLAARYPHQLSGGQQQRVAIARALVYEPPV
                     ILLDEPLSNLDAKLREEARAWLRTLIVTLGLSAIHVTHDQVEAMAIADRVVLLDAGLI
                     AQDGPPTTLYNEPATLFAAEFMGANNRLNGTLTENSG"
     gene            complement(759..>2105)
                     /locus_tag="VFL49_00505"
     CDS             complement(759..>2105)
                     /locus_tag="VFL49_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7910488.1"
                     /translation="VVIAFGYVVAIGPVGIFSTLAKDILGFVPWNLYSLLALIVIAGL
                     THVPHVYLYSAAALRGLSTDLEEAARVSGANPFRVAVNVSLPMILPAILFAGVLVFFL
                     GFELFGLPLVLGDPQDLLVLSTYLFKLTNKLGVPSYQLMAVVVVAIIAITAPLVFMQR
                     LLLRQAQRYVSVRGKGLKTQPLPLHRWRWVAFAGIILWLFVTVVVPISGITLRSFVVT
                     WGEGVRLLDVLTLDHYRELTDYPNVVRGIFNTLGIGVIGGAVAVACYTAIALAVHRWN
                     SGWTRLVDYLVMVPRAMPGLVAGLALLWVFLFVPFLSPLKSTMVSIWLAYSIVWLAYG
                     MRLVSGTLLQVAPELEEAARTAGASDLRVKRDVTVPLIKYGMLASWLLVFLIFVREYS
                     TGIYLLGPGTEVIGSLLVSLWGTGAVDLVSALSVVNVVMIGVGLAVAVRLGVRLHG"
BASE COUNT          475 a          650 c          673 g          307 t
ORIGIN      
        1 caccggaatt ctcggtcagc gtgccattga gccggttgtt cgcgcccatg aattccgcgg
       61 cgaaaagcgt cgccggctcg ttgtagagcg tggtgggcgg gccgtcctgg gcgatcaatc
      121 cggcatcgag caacacgacg cgatcggcga tcgccatggc ctcgacctgg tcatgcgtga
      181 cgtgaattgc cgacaatccc aaagtgacga tcagcgtgcg cagccacgca cgcgcttcct
      241 cgcgcagctt ggcgtccaga ttggacaatg gttcatcgag aaggatcacg ggcggctcat
      301 aaacaagagc acgcgcgatc gcgacgcgct gttgctggcc acctgaaagc tggtgcggat
      361 agcgcgcggc gagatggccg agtccgatct gcgacagcgt cttctcgacg cgagccttga
      421 tttcctcggc cgggataccg cgcagcttga ggccataggc gacgttgtcg aatacggtgc
      481 ggtggggcca cagcgcgtag gattggaaca ccagcccgag gccgcgcttc tccgccggca
      541 aatcgatctc gcggcttgcg tcgaaaaata ccgtgtttcc gatcgcgatc gaaccctgat
      601 gcggcgtctc cagaccggca acggcgcgca gcaaagtcgt cttgccgctg cccgatgggc
      661 cgagcagggc gaccacttcg cctgccggca ctgaaagcga aacgccttta agtatctcgt
      721 tgtcgccgag tcgaagggcg acgtccttga cgatgagctc agccatgcag gcgcactcca
      781 aggcggaccg cgacggcaag gccgacgccg atcatgacca cattaacgac tgataacgcc
      841 gataccagat cgaccgcgcc ggtgccccag agcgacacca gaagcgagcc gattacctcg
      901 gtgccgggac caagcaggta aatgccggtc gagtattcgc gcacgaagat caggaagacc
      961 agcagccagc tcgccagcat gccgtatttg atcaacggta cggtgacgtc gcgcttgacg
     1021 cgcagatcgc tcgcgccggc ggttcgcgcg gcttcctcaa gctccggtgc gacctgcagc
     1081 agcgtgccgg aaacgagccg catgccataa gcaagccaga cgatggaata ggcaagccag
     1141 atcgaaacca tcgtcgactt gagcggcgac aggaacggca cgaacaggaa gacccataaa
     1201 agcgccaggc cggccacgag gcccggcatg gcgcgcggga ccatcaccag atagtcgaca
     1261 aggcgggtcc agcccgagtt ccagcgatgc accgcaagcg caatcgcggt gtagcaggcg
     1321 acggccacgg cgccgccgat gacgccgatg ccgagcgtgt tgaagatgcc gcgcaccacg
     1381 ttcggataat cggtgagctc gcggtagtga tcgagcgtga gcacgtcgag cagcctcacg
     1441 ccttcacccc aggtcacgac gaacgagcgc agcgtaatcc cggagatcgg cacgaccacg
     1501 gtcacaaaaa gccagagaat gattccggca aaggcgaccc agcgccagcg atgcagcgga
     1561 agcggctgcg tcttcaggcc tttgccgcgc accgacacat agcgctgggc ctggcgcaac
     1621 agcaggcgct gcatgaaaac cagcggcgcg gtgatcgcga tgatggcaac gacgacaacg
     1681 gccatcagct gatacgatgg cacgccgagc ttgttggtga gcttgaacag ataggtcgac
     1741 agcaccagca gatcctgtgg atcgccaagc accagcggca ggccgaacag ctcgaagccc
     1801 agaaagaata ccagaacgcc ggcgaacagg attgcaggca ggatcatcgg caggctgaca
     1861 ttgacggcga cgcggaaggg gttggcgccg gacacccgcg cggcttcttc aagatcggtg
     1921 ctcaggcctc gcagtgcggc cgcgctgtag aggtagacat gcgggacgtg ggtaagaccg
     1981 gcaatcacga tcagcgcgag caatgaatag agattccacg gcacgaagcc gagaatgtcc
     2041 ttcgcgagcg tggagaaaat tccgaccggg ccgatggcca cgacgtaccc gaacgcgatc
     2101 accac
//
LOCUS       DASSRL010000026         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1208136, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000026 DASSRL010000000
VERSION     DASSRL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208136"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFL49_00510"
     CDS             <1..943
                     /locus_tag="VFL49_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7910489.1"
                     /translation="PGFFGSLAATPQQPGFSYASIYYHTSVSAGGNVAFARQVTAGRL
                     TANFNGNLNANLDASADLYMGIPQYVFATPVLGGQAAIAAAVPYGRSRGSVDATLTGS
                     LGPLGFNVSGSRDDAVTGWGDIAPMFTLRWNQGVHNFMTYLTGNLTTGRYDATRLANL
                     GIGHNAIDGGGSYTYFDPQKGHEFSATLGFTYNFENVHTDYQNGIDMHLDWGASQFLS
                     KQLQVGLVGYLYNQLSCDSGSGDRVGCFKSRVAGIGPQIGYIIPISNDYQGYMNLKGY
                     KEFAAENRADGWNLWLTFAISPAEKRPPTAARPMITK"
     gene            975..2207
                     /locus_tag="VFL49_00515"
     CDS             975..2207
                     /locus_tag="VFL49_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7910490.1"
                     /translation="MPQNIFCTSGALRKLRTGICVSVGLLAAVAVSGCATLERLPAVT
                     YAEARQLDILDIPDARFYVSDVNRIYDVAIKAYQRSNRVRPAHTRNFLALSGGGDDGA
                     FGAGLMVGWSAHGDRPQFDMVTGVSTGALSAPFAFLGRAYDQKLAGMYTETNAGDIFQ
                     RRPVLIAAVASDSLVDNTPLRKLIESSVDAAMVRHIAEEYGKGRLLFVLTTNLDQSRP
                     VVWNIGAIAASNSPQARELIVNVLLASASIPAVFPPVMLDVTVDGQKRQEMHVDGGTI
                     AQVFFYPPSFSLKGAAARLGIDAQKLRARKRVAYVIRNGRFFRPEESVQLRTLAIAKE
                     ALSTMTMSSGINDTYRMYTLARRDGVDFNLASIGEDFTVPYKGPFDPGYMQALFAYGY
                     EKGRAGYPWKKVPPGYSN"
     gene            2329..2715
                     /locus_tag="VFL49_00520"
     CDS             2329..2715
                     /locus_tag="VFL49_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910491.1"
                     /translation="MLIVATLPSLADSGTVRLRIGSAGFIIGAGGGSGTLTFHGKTYP
                     LRIGGVSLGMIGVSSADLVGRARNLRRASDIAGSYTAVGAGLAVAGGAATARLQNANG
                     VVLEVKGTKVGFSASVNLSGLTISMQ"
     gene            2936..3670
                     /locus_tag="VFL49_00525"
     CDS             2936..3670
                     /locus_tag="VFL49_00525"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7910492.1"
                     /translation="MRLHHFAFPLFAFPVAAIIALLTFAQAAHANVMIIIDKSAQKMT
                     VTVNGEDRYTWPVSTGRNGYDTPSGDFQPFRMEKDHFSREWDDAPMPNSIFFTKIGHA
                     IHGTYEVRNLGKPASHGCVRLAPQNAATLYALVKDEGVFNTRVRLIGEIPKSSDLVAS
                     RDRGNSTVREPARNPDSDQPRRSYVRNYTTDDADADDAPPPQRNYQVRSGRETQYGPR
                     YYYRERPYPYYERRTYGRGLFGSSGW"
BASE COUNT          758 a         1191 c         1143 g          723 t
ORIGIN      
        1 gccgggattt ttcggaagcc tggcggcgac accgcaacag ccgggatttt cgtacgcatc
       61 gatttattat cacacgtccg tatcggcagg gggcaatgtg gccttcgcgc ggcaggtcac
      121 cgccggccgc ctcaccgcga atttcaacgg aaatctgaat gcaaatctcg atgcgagcgc
      181 cgatctttat atgggcatac cgcagtacgt atttgccact ccggtgctcg gcggccaagc
      241 ggctattgca gctgcggtgc cgtacgggcg cagccggggt tctgtcgatg ccacgctgac
      301 cggatcgctg ggccctctgg gcttcaatgt ttcaggcagc cgggacgatg cagtcaccgg
      361 gtggggcgat atagcaccaa tgtttacgct gcgttggaat cagggcgtgc ataatttcat
      421 gacctacctt acaggcaatc tgacaacggg ccgctacgac gcgacgcggc ttgccaatct
      481 gggcatcggc cacaatgcga tcgatggcgg cggcagctac acctacttcg acccgcaaaa
      541 gggacatgaa ttctcggcca cgctcggctt cacctataat ttcgagaacg tgcacaccga
      601 ttaccagaac ggcatcgaca tgcatctgga ctggggtgcc tcgcagtttc tgtcaaagca
      661 actgcaggtc ggacttgtcg gctatttgta caaccagctg tcctgtgaca gcggctcggg
      721 cgatcgtgtc ggctgcttca agtcgcgggt cgcgggcatc ggaccgcaga tcggctacat
      781 cattcctatc agcaatgact accagggcta catgaacctg aagggctaca aggaattcgc
      841 agcggaaaat cgcgccgacg gctggaacct ctggttgacg tttgcgatct cgccggcgga
      901 aaaacggccg ccgacagctg ccaggcccat gatcacgaaa taacagacgc gggcgtatcg
      961 gggcgcgtag caatatgcct cagaatattt tctgcacgtc gggtgcgctt cggaagctgc
     1021 gcaccggcat ttgcgtgtcc gtcggcctgc tcgcggccgt cgccgtttca ggctgcgcga
     1081 cacttgaacg cctgccggcc gtgacctacg cggaggcccg gcaactcgac attctcgaca
     1141 ttcccgacgc gcgtttttac gtgagcgacg tcaaccggat ctacgatgtc gcgataaagg
     1201 cctatcagcg cagcaaccgc gtgcggccgg cacacacccg caatttcctt gcgctgtccg
     1261 gcgggggcga cgacggcgca ttcggagccg gtcttatggt cggatggagc gcgcacggcg
     1321 accggcctca attcgacatg gtgaccggcg tcagcacggg agcgctttcc gcgcctttcg
     1381 ccttcctggg ccgcgcctac gaccaaaaac tggccgggat gtataccgaa acgaatgcgg
     1441 gcgatatctt ccagcggcgc ccggtgctga tcgctgccgt tgcctccgat tccctcgtcg
     1501 acaacacgcc gctgcgcaaa ctcatcgaaa gcagtgtcga tgcggcgatg gtccggcaca
     1561 ttgccgagga atacggtaaa ggccggctgt tgttcgtgct gacgacgaac ctcgaccaga
     1621 gccgtcccgt cgtctggaat atcggggcga ttgccgcaag caatagtccg caggcccgcg
     1681 aactcatcgt caacgttctg ctggcgtcgg cctcaatccc cgccgtgttt ccgccggtca
     1741 tgctggatgt caccgtcgac ggccagaagc gccaggaaat gcacgtcgac ggcggaacga
     1801 tcgcgcaggt gtttttctac ccgccctcgt tctccctcaa aggcgccgct gcacggcttg
     1861 gcatcgacgc gcagaagctg cgggcgcgca agcgcgtcgc ctacgtgatc cgcaacggcc
     1921 ggttcttccg tcccgaagaa agcgtgcagc tgcggaccct ggcgattgcc aaagaagcgc
     1981 tgtcgacgat gaccatgtcc agcggtatca atgacaccta tcgcatgtat acgcttgcca
     2041 gacgcgacgg cgtcgatttc aatcttgctt cgatcggcga agatttcact gtgccttaca
     2101 aagggccctt tgatccaggc tacatgcaag cgcttttcgc ctatggatac gaaaaaggcc
     2161 gagccggcta tccatggaaa aaagtgccgc cgggctactc gaactgagac ataagtcagg
     2221 atgctttccc aaagcacatt ctttcccgat gcaacaacca aggaggcaat gatgattggt
     2281 tttacgcgta cgattaagtg tgccgcagtg gcatttgcgg cagcggccat gttgattgtg
     2341 gcaacgctgc cgtcgctggc agacagcggc acggtgcggc tccgcatcgg cagcgccggc
     2401 ttcattatcg gcgccggcgg cggctcgggt accttgacct ttcatggcaa gacttacccg
     2461 ctgcgtatcg gcggcgtcag cctcggcatg atcggcgtga gcagcgccga ccttgtcggc
     2521 cgtgcgcgca atctgcgcag ggccagcgat atcgcaggtt cttacacagc ggtgggtgcc
     2581 ggccttgccg ttgcgggagg cgcggcaact gcccggctgc agaatgcgaa tggcgtcgtg
     2641 cttgaggtca aaggcaccaa ggtcggattt tcggcgtcgg tcaatctcag cggcctgacg
     2701 atatcgatgc aatagaccgc cagcgctcgt cgcactggaa cgagacttct tcgacggcgt
     2761 tacctctctg gccccggcag cgaatacgcc ggggccggcg catcgtcggt gcgatgcaag
     2821 acggtatcgt taccctcccc tcgcccgcac gaaatgacta ttgcgtgaat tgacgtaggc
     2881 aacggctgcg cgcgtgctag gctgcagcgg tcggggttga agttggagtc ttcgtatgcg
     2941 tctccaccat ttcgcattcc ccttatttgc gtttcccgtg gcggcaataa ttgcactcct
     3001 gacattcgct caagccgctc acgccaacgt catgatcatc atcgacaagt cggcgcagaa
     3061 aatgacggtc accgtcaacg gcgaggaccg ctacacctgg ccggtgtcga cgggccgcaa
     3121 cggttacgac acgccgtcag gcgattttca gccgttccgc atggagaagg accatttctc
     3181 gcgcgaatgg gacgatgcgc cgatgccgaa ctcgattttc ttcaccaaga tcggccatgc
     3241 cattcacggc acttacgaag tccgcaatct cggcaagccg gcctcgcatg gctgcgtgcg
     3301 gctggcgccg cagaacgccg cgaccctcta cgccctggtc aaggacgaag gcgttttcaa
     3361 cacgcgcgtg aggctgatcg gcgaaattcc gaaatccagc gatctcgtcg caagccgcga
     3421 ccgcggcaac agcaccgtgc gcgagccggc ccgcaacccg gacagcgacc agccgcgccg
     3481 gagctacgtg cgaaattaca ccacagacga cgccgatgcg gatgacgcgc cgccgccgca
     3541 acggaactac caggtgcgca gcggccgcga aacgcaatat ggcccgcgct actactaccg
     3601 cgagcgcccg tatccttatt atgagcgccg cacgtacggc cgcggcctct tcggctcgtc
     3661 cggctggtga cggcatgatc cgcaagcttg cgggcgggaa atacaggctc tattcgcgca
     3721 agaagaacgc gcgcaccggc aagcgccgca acctcggcac gttcagctca aaacgggcgg
     3781 cgcagaagca cgagcgcgac atccagtact tcaag
//
LOCUS       DASSRL010000027         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_210823, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000027 DASSRL010000000
VERSION     DASSRL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210823"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..524)
                     /locus_tag="VFL49_00530"
     CDS             complement(114..524)
                     /locus_tag="VFL49_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET7910493.1"
                     /translation="MSAFHFELVSPEKLLFSGDVEQVDVPGAEGDFGVLADHAPMVAT
                     LRPGILTVHAGGGTQKIVVLGGFAEVSSEGLTVLADLAEAVEDIDRTVVTTRIGELES
                     RIAKTEPGSELDKLMTRLDHYKEVDRHLTGTAMH"
     gene            complement(532..786)
                     /locus_tag="VFL49_00535"
     CDS             complement(532..786)
                     /locus_tag="VFL49_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910494.1"
                     /translation="MFGRSNLVAFTVVGLLIGALAGYLTRPESSEIQLGPLKIEVTGD
                     RPARDGGPLTSKQVEHIALITLIGGVIGLGLGFASGRNRG"
     gene            complement(790..2226)
                     /gene="atpD"
                     /locus_tag="VFL49_00540"
     CDS             complement(790..2226)
                     /gene="atpD"
                     /locus_tag="VFL49_00540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7910495.1"
                     /translation="MATPNAVGKITQVIGAVVDVQFEDHLPAILNALETENGGNRLVL
                     EVAQHLGESTVRTIAMDSSEGLVRGQDVTDTGQPIEVPVGNETLGRIINVVGEAVDEA
                     GPVKTKEKRAIHQPAPEYIEQSTEAQILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKT
                     VLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVNKDPKKGSVEGSKCAL
                     VYGQMNEPPGARARVGLTGLTVAEYFRDQGQDVLFFVDNIFRFTQAGSEVSALLGRIP
                     SAVGYQPTLATDMGALQERITTTTKGSVTSVQAIYVPADDLTDPAPATSFAHLDATTV
                     LSRDIAAKGIYPAVDPLDSTSRMLSPLIVGEEHYAVARQVQQILQRYKSLQDIIAILG
                     MDELSEDDKLTVARARKIERFLSQPFHVAEVFTGSPGKLVDLQDTIKGFKGLCDGKYD
                     HLPEAAFYMVGTIEEAVEKGKKLAAEAA"
     gene            complement(2245..>2609)
                     /locus_tag="VFL49_00545"
     CDS             complement(2245..>2609)
                     /locus_tag="VFL49_00545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET7910496.1"
                     /translation="VCTLFFSRFKSVIAQIPTAQQIIPPVFEEGAENGANATYEYEPE
                     EDEILAELLPRNISVQVFRALLENAASEQGARMSAMDNATRNAGEMIRKQTLTYNRTR
                     QAMITKELIEIISGAEAL"
BASE COUNT          472 a          810 c          782 g          545 t
ORIGIN      
        1 tgaatttgcg cgatctttaa gacgtggatg cccgcgacat aggcgtgcga aagcacgccg
       61 tccttcggac ggctatgcgc gggcatgacg attcaatttg aaacgatcct gctctagtgc
      121 atggctgtgc cggtgagatg ccggtcgact tccttgtaat gatcgagccg cgtcatcagc
      181 ttgtcgagct ccgaaccagg ttcggttttc gcgatgcggc tttctagttc gccgatgcgc
      241 gtcgtaacca ccgtacggtc aatatcttcg acggcctcgg ccaagtcggc caataccgta
      301 agtccttcag acgatacttc ggcaaaacct ccaagcacga cgatcttctg tgttccgccg
      361 ccggcgtgaa cggtcagaat gccggggcgc aaagtcgcca ccatcggcgc gtgatcggca
      421 agcacgccga aatcgccttc ggctcccggc acgtccacct gctcgacgtc gccggaaaac
      481 agcagtttct ccggcgaaac cagctcaaaa tgaaatgcag acattgcgca atcagcccct
      541 gttcctgccg gacgcaaatc ccaatccgag cccgatgacc ccgccgatca gcgtgatcag
      601 cgcgatgtgt tcgacctgtt tcgaggtgag cggcccgcca tcgcgggcag ggcgatcccc
      661 cgtcacctcg atcttcaacg gccccagttg tatttccgac gattccggac gtgtcagata
      721 gccggccaag gctccaatca gcaggccaac cacggtaaac gcgaccagat tcgatcggcc
      781 aaacatgtat tacgcggctt ccgccgcaag cttcttgcct ttttcgactg cctcttcgat
      841 ggtgccgacc atgtaaaagg cggcctcggg caagtgatcg tatttgccgt cgcagagccc
      901 tttgaagcct ttgatcgtat cctgcagatc gacgagcttt ccgggcgaac cggtgaacac
      961 ttccgcgacg tggaacggtt gcgagaggaa gcgctcgatc ttgcgcgcgc gggcgaccgt
     1021 caatttgtcg tcttccgaca attcgtccat gccgaggatg gcgatgatgt cctgcagcga
     1081 cttgtagcgc tgaagaatct gctgcacctg ccgcgcgacg gcgtagtgct cctcgccgac
     1141 gatcagcggt gacagcatgc gcgaggtcga atcgagcggg tcgaccgccg gatagattcc
     1201 cttggcggcg atgtcgcgcg agagcacggt ggtcgcatca agatgggcga aagaggtcgc
     1261 cggcgccggg tcggtaaggt cgtcggccgg cacgtagatc gcctgcacgg acgtgacaga
     1321 acccttggtg gtcgtcgtga tgcgctcctg cagcgctccc atgtcggtcg caagcgtcgg
     1381 ctgatatccc accgcggatg gaatacggcc gagcaacgcc gacacttccg agccggcttg
     1441 cgtgaagcga aagatgttgt cgacgaagaa caacacgtcc tgtccctgat cgcggaaata
     1501 ttcggcgacc gtcagtccgg tcaggccgac gcgggcgcgg gctccgggcg gctcgttcat
     1561 ctgaccgtac accagcgcgc actttgagcc ttcgaccgac cccttcttcg ggtccttgtt
     1621 gacgccggat tcgatcatct cgtgatagag gtcgttgccc tcgcgcgtcc gctcgccgac
     1681 gccggcgaac accgaataac cgccgtgcgc cttggcgacg ttgttgatga gttccatgat
     1741 cagcacggtc ttgccgacgc cggcgccgcc gaaaaggccg atcttgccgc ccttcgcgta
     1801 aggcgcgagc aggtccacga ccttgatgcc ggtgacgaga atttgcgctt ccgtcgactg
     1861 ctcgatgtat tccggcgccg gctggtgaat ggcgcgcttc tccttggttt tcaccggacc
     1921 tgcctcgtcc accgcctcgc ccaccacgtt gatgatgcgg ccgagcgtct cgttgccgac
     1981 cggcacttcg atcggctggc ctgtatcggt gacatcctga ccacgcacca ggccttcgga
     2041 tgaatccatc gcgatggtgc gcacggtcga ttcaccgaga tgctgcgcca cttcgagcac
     2101 caggcggttg ccgccattct cggtctcgag cgcgttcagg atcgccggca gatgatcctc
     2161 gaactgcacg tcgacgacgg cgccgatgac ctgcgtgatt tttccgactg cgttgggtgt
     2221 agccatgtcg tcctcgttac ggaattaaag cgcttcggcg ccggagatga tctcgatcag
     2281 ctccttggtg atcatcgcct gacgggtgcg gttgtaggta agggtttgct tgcggatcat
     2341 ttcgccggca ttgcgcgtag cgttgtccat cgcgctcatg cgggcgccct gctcggaggc
     2401 ggcattctcg agcaacgcgc ggaatacctg tacggagatg ttccgcggca gaagctcggc
     2461 gaggatctca tcctcttccg gctcatattc gtacgtggcg ttcgcgccgt tttcggcgcc
     2521 ttcttcgaac accggcggaa tgatctgctg tgccgtcggg atttgcgcga tcaccgactt
     2581 gaaacgggaa aagaacagcg tgcagacgt
//
LOCUS       DASSRL010000028         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1645602, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000028 DASSRL010000000
VERSION     DASSRL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645602"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFL49_00550"
     CDS             complement(<1..770)
                     /locus_tag="VFL49_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7910497.1"
                     /translation="MDRPWLRHYPPGVPGEIEASRYPSLVAMFEESFQKYSEREACAC
                     MGKSLTYAAVEEASRALAAWLQGLGLKRGARVAIMMPNVLQYPIAIAAILRAGCTVVN
                     VNPLYKPRELEFQLADAGAEAIIVLENFAGVLQQALPNLQVKHVVIASLGDMLGPVKG
                     AIVNAVVRHIKKMVPDYSIPGATRFNDAVAAGRKMALEVSSSEPDDIAFLQYTGGTTG
                     VAKGAMLLHRNLVANMLQVEAWNQPMVDIPPKVDHMIIV"
     gene            complement(869..1996)
                     /locus_tag="VFL49_00555"
     CDS             complement(869..1996)
                     /locus_tag="VFL49_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7910498.1"
                     /translation="MSEPAPPAERKTPPTPIPFTVLTGFLGAGKTTLLNRLLKDPALA
                     DTAVVINEFGEVGLDHLLVEYVSDNMVLLQSGCLCCTMRGDLVDALETLLRDLDNRRC
                     TFKRLLLETTGLADPAPVLHTAMSHPYLVLRYRLDGVITVVDAATGEATLDAHAEAVK
                     QAAVADRIVLTKTDLVDETQRQRIVARLRGLNPAAPILEAANGEATTEKLLGSGLYDP
                     DRKIPDVKKWLAAEAYAGGHGHHHDHHHHDVNRHDEHISSFVLTAADAIPSGTLEMFL
                     ELLCATHGEKLLRVKGIVKLAEMPDTPVVVHGVQHIFHPTARLDRWPDDDRRTRLVFI
                     TRDLPQRTVQELFEAFLGVAAPDRPDRTALVDNPLVPFGGR"
     gene            complement(1993..3252)
                     /locus_tag="VFL49_00560"
     CDS             complement(1993..3252)
                     /locus_tag="VFL49_00560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7910499.1"
                     /translation="MILGLAAAVLALVTAERARAEAQLLIEASTGKVLHAENATYPWY
                     PASVTKLMTAYTTLRAVKEGRITLNTLLTISRNAAAQQPTKMGFKVGTTVTVDNALKM
                     LMVKSANDIAVAIAEGVGGSIPGFADLMNANARRLGMSQSNFVNPNGLPAENHISSAR
                     DLGILARALIREFPEYDSYWHISSIRYGNRVMRNYNSLIDRYPGADGMKTGFICASGY
                     NVVASATRNGRRLIAVILGSWSGAVRAQKAAQLLERGFNSGGLSWLTPSLGTVDALAP
                     IDAQPPNLREEMCGGHRRKPPSEENEEEPEESTTAANGESENGQAFMLSSLKPANGKF
                     VLGPPVETTPPVVVFTGPADHPDPVAQTASATPKKKKKAAAKADGAEKPAKGAAAEKS
                     KKTDDAKPAKTAKPAKPAKPKVTSTAQ"
     gene            3452..>3584
                     /locus_tag="VFL49_00565"
     CDS             3452..>3584
                     /locus_tag="VFL49_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET7910500.1"
                     /translation="MTACIVGWAHSPFGKLAGETLESLIVKVAGEALADAGIAAKDVD
                     "
BASE COUNT          631 a         1066 c         1172 g          715 t
ORIGIN      
        1 acgatgatca tgtgatcgac tttcggcggg atgtcgacca tcggttgatt ccatgcttcc
       61 acctgcaaca tgttggcgac cagattgcga tggagaagca tcgcgccttt cgcaactccc
      121 gtggtgccgc cggtgtattg gagaaatgca atatcgtccg gctctgacga cgacacttcc
      181 agcgccatct tgcgacccgc cgcaacggcg tcgttgaatc ttgtagcgcc cgggatcgag
      241 tagtccggca ccatcttctt gatgtggcgg acgacggcgt tgacgatcgc gcccttcact
      301 gggccgagca tgtcgcccag gctcgcgatg accacatgct tgacctgcag atttggcaat
      361 gcctgttgca aaacgcccgc aaaattttcc agcacgatga tggcttcggc gcccgcatcg
      421 gcaagctgga attccagttc gcgcggcttg tagagcggat tgacgttcac gacggtgcag
      481 ccggcgcgca ggatggcagc gatggcgatc ggatattgca gcacgttcgg catcatgatg
      541 gcgacgcgcg cgccgcgttt caggcccagg ccttgcagcc acgccgcaag cgcgcgtgaa
      601 gcttcttcca cggcggcata ggtcagggac ttgcccatgc aggcacaggc ttcacgctcg
      661 ctatatttct ggaagctttc ctcgaacatc gcgaccagcg acgggtaccg cgacgcttca
      721 atctcgccgg gcacgcccgg cggatagtgc cgaagccaag gccggtccat gatgtttccc
      781 ccgcgaccgt cttttctcgc aatcgcgccg acggtcgatt ccatgtcagc atgagtccaa
      841 ccgggcccgc atgcaagacc gccaatcgct agcggcctcc aaacggaacg agcgggttgt
      901 cgacgagggc ggtccgatcg ggccggtcgg gcgccgcaac cccgagaaag gcctcgaaaa
      961 gctcctgcac ggtgcgctgc ggcaaatcgc gggtgatgaa aaccagccgg gtgcgccggt
     1021 cgtcgtcggg ccagcggtcg agccgagctg tcgggtggaa tatgtgctgc acgccgtgca
     1081 caacgaccgg cgtatccggc atttctgcga gcttgacgat gcctttgacg cgcaaaagct
     1141 tttccccgtg cgtggcgcac aacagctcga gaaacatctc cagcgttccc gacgggatcg
     1201 cgtcggcggc tgtgagaaca aaagagctga tgtgctcgtc gtgccggttg acgtcgtgat
     1261 gatgatggtc gtggtgatgg ccatggccgc cggcataagc ttcggcggca agccattttt
     1321 tgacgtcggg gattttccgg tcgggatcat aaagcccgct tccgagcagc ttttcagtgg
     1381 tcgcttcgcc gttggcggcc tcgaggatag gcgcagccgg attgaggccg cgaagccggg
     1441 caacaattcg ttgccgttgc gtctcatcga caaggtcggt cttggtcagc acgatacggt
     1501 cggcgacggc ggcctgcttc accgcctcgg cgtgcgcatc gagcgtcgct tcgccggtgg
     1561 cggcgtccac gacggttatg acgccgtcca gacggtagcg taagacgaga tagggatgcg
     1621 acatcgctgt atgcagaacc ggcgccggat cggcgaggcc cgtggtttcg agcaagagcc
     1681 gcttgaatgt gcagcgccgg ttgtcgaggt cgcgcaacag tgtttccagc gcgtcgacga
     1741 ggtcgccacg catcgtgcag cacaggcagc ccgactgcag cagcaccatg ttgtcgctga
     1801 catattccac cagcaggtgg tcgaggccga cctcgccgaa ttcgttgata acgaccgcgg
     1861 tgtccgcgag cgccggatcc ttcagcagcc ggttgagcag ggtcgtcttc ccggcgccca
     1921 ggaagccggt gagcacagtg aacggaatcg gcgtcggcgg ggtctttcgc tcggcgggcg
     1981 gggcaggctc gctcattggg ccgtcgaggt gactttcggt ttcgccggct ttgccggctt
     2041 ggcagtcttt gccggcttcg cgtcatcagt ctttttggac ttttcggccg ccgcgccctt
     2101 ggcgggcttt tcagcgccgt cggccttggc ggcagccttc tttttcttct tcggcgtcgc
     2161 gctcgcggtc tgcgcaaccg ggtcgggatg gtcggccgga cccgtgaata ccaccacggg
     2221 cggcgtggtc tcgaccggcg gtccgagcac gaatttgccg ttggccggct tcaggctgga
     2281 gagcatgaac gcctgcccgt tttcagattc gccgttcgcg gcggttgtcg actcttccgg
     2341 ttcttcctcg ttttcctcgg agggtggttt gcgacgatgt ccgccgcaca tttcttcacg
     2401 caagttaggc ggttgcgcat cgatcggcgc cagcgcgtcc accgtaccga gcgacggcgt
     2461 gagccacgac aggccgccgc tgttgaagcc gcgctcgaga agttgcgcgg ccttttgtgc
     2521 gcgcactgcg ccggaccatg atccgaggat caccgcgatc agccggcgac cgttgcgggt
     2581 ggcggaggcg acgacgttgt agcccgaggc gcagatgaag ccggtcttca tgccatcggc
     2641 gccgggataa cgatcgataa gcgagttgta gtttcgcatc acgcggttgc cgtaacggat
     2701 cgacgaaatg tgccagtaac tgtcgtactc gggaaactcg cggatcagcg cgcgtgccag
     2761 gataccgagg tcgcgggccg aggagatgtg gttctccgca ggcaggccgt tcggattgac
     2821 gaagttgctc tgtgacatgc cgagacggcg cgcgttggca ttcatcagat cggcaaagcc
     2881 cgggatggag ccgccgacgc cctcggcgat cgctaccgcg atgtcgtttg cggatttcac
     2941 catcagcatc ttcagcgcat tgtcgacggt gacggtggtg ccgaccttga agcccatttt
     3001 cgtcggctgc tgtgcggcgg cattgcgcga tattgtcagc agcgtgttga gcgtgatgcg
     3061 accttccttc accgcgcgta gcgtggtgta ggcggtcata agttttgtga ccgaagccgg
     3121 ataccacgga taggtggcgt tctcggcatg caacaccttg ccggtcgacg cttcgatcag
     3181 cagttgcgct tccgcgcgcg cgcgttcggc cgtgacaagc gcaagcacag ccgcggcgag
     3241 gccgaggatc atgacgcgac ggcaatggct tgaacggaca gtcattcgcg gttcagaatc
     3301 ccaaggcctc agaaacggga aagttccata gacagccgtg ctaggctatc taagcttcga
     3361 atgctaggca aagcgcgtga accgccgctg aatataccgg gtatcagcag agcccgatga
     3421 aaacgaccaa agttcggcaa aggaggcgtt aatgacggcc tgtatcgtcg gttgggcgca
     3481 cagtcccttc ggcaagcttg ccggtgaaac gctcgaaagc ctgatcgtga aggttgcggg
     3541 cgaggcgctg gctgatgccg ggattgcggc caaggacgtc gacg
//
LOCUS       DASSRL010000029         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_245853, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000029 DASSRL010000000
VERSION     DASSRL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245853"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..727)
                     /locus_tag="VFL49_00570"
     CDS             complement(74..727)
                     /locus_tag="VFL49_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HET7910501.1"
                     /translation="MNVSIDQILLRMTALCMSLILLAGNGALAADAPASAVSSGPVTA
                     PADSKVAGDYVIGPGDTIQVFVWRNPELTTTVPVRPDGKISTPLVEDMTAVGKTPSDL
                     ARDIEHVLAEYVRSPQVNVIVTQPASVFSQIKVIGQVTRPQSVPYRDGLTVLDAVLAV
                     GGLGPFAAGNRSKIVRTENGKTKEIKVKIASLVNDGDMSQNLHLRPGDVIVIPESRF"
     gene            complement(861..>2032)
                     /locus_tag="VFL49_00575"
     CDS             complement(861..>2032)
                     /locus_tag="VFL49_00575"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7910502.1"
                     /translation="DTSNTAPRLALVELALREGDAAGATQQLEQMRAADPKAVEPRLA
                     LIRLYMQQKKTRDADTIVREIQSRGADAATSQALGRIYLDAGRFDEALNFFRNAVSQD
                     AGNPSYAMDVARAQLALGNTGSARETLQKSAQANPQSVAAAATLVLLDLREGRRDSAM
                     SRLEALKQAHPHDASVAVLEGEVAAHAGDYSSAAAAYDRAYRLSPSAGTALRSYRART
                     QARQPDAPSLLEDWLRKHPGDESLRMVLAEAYMQSGKQSQAIAQYERLADNAKPHPMA
                     LNNLAWLYQQNSDPRATEVAKRAYEAAPAVPAIADTYGWILVGSGRIEEALPVLERAA
                     SAKDVAPDIRYHYAVALTKAGRKPEARAVLEPVVAQAGNSPVGAAARKLLGELGG"
BASE COUNT          350 a          658 c          624 g          400 t
ORIGIN      
        1 atagcgtctg aatcgccatg cgccgtgggc gtactcgatg atctgatcga gaatttcttg
       61 catgagttct tgtctagaaa cgcgattcgg gaatcacgat gacatcgccc gggcgcaggt
      121 gcaggttctg actcatgtca ccgtcgttga ccagtgaggc gatcttgacc ttgatctctt
      181 tcgtcttgcc attttcagtg cgcacgatct tggaccgatt gcctgccgca aacggaccca
      241 gaccgcctac ggcgagcacc gcgtcgagca cggtcagacc gtcgcgatac ggcaccgatt
      301 gcggccgcgt gacctggcca atgaccttga tctggctgaa gacgctcgca ggctgggtca
      361 cgatgacatt gacctgcggc gaacggacgt actcagcgag cacatgttcg atgtcgcggg
      421 cgaggtcgga tggcgtcttg ccgaccgccg tcatgtcctc gaccagcggc gtcgaaatct
      481 tgccatcggg ccgtaccggc acggtggtcg tcagttccgg gttgcgccag acgaaaacct
      541 ggatggtgtc gcccgggccg atgacgtaat cccccgcgac cttgctgtcg gctggagccg
      601 ttacgggccc gctgctgacc gcactcgcgg gcgcgtccgc ggccagggcg ccgttgcctg
      661 caagcagaat caacgacatg cacaaggccg tcatacgcaa aagaatctgg tcgatggaga
      721 cgttcattcg gacagcaaac cgtttgacag aatcgaccga gcgcgcagcg ccggaccctg
      781 ggtgatttgt cgcaagagta tcagatacct gcatggcacg acttcaagct gttactcaca
      841 tctgaagggt gactccccga ctacccgccc agttccccca gcagcttccg tgcggccgcg
      901 ccgacgggtg aatttcctgc ctgggcaacg acaggttcca gtacggcacg agcctctggc
      961 tttcgtccgg ccttggtcaa tgcgaccgcg tagtggtatc ggatgtccgg agcgacgtcc
     1021 tttgccgatg ctgcccgctc gagaacaggc agcgcctcct caatgcgtcc cgaccccacc
     1081 aggatccatc cataagtgtc agcaatggcg ggtactgccg gtgccgcttc ataggctcgc
     1141 ttggcgacct ccgtggcgcg cggatcgctg ttctgctggt acagccacgc cagattgttg
     1201 agcgccatcg gatgaggctt tgcgttgtcc gccagcctct cgtactgggc gatcgcctgc
     1261 gactgcttgc cgctctgcat ataggcctcc gccagcacca tgcgtaacga ctcgtcaccg
     1321 ggatgcttgc gcagccagtc ctccagcagg gacggcgcgt caggctgtct ggcctgagtg
     1381 cgcgcgcggt aagaacgcaa tgccgtaccc gccgatggcg acaatcggta cgcgcggtcg
     1441 taagcggcgg cggcgctgct gtaatccccg gcgtgggcgg ccacttcgcc ttcaagcacc
     1501 gcaacgctcg cgtcgtgcgg atgcgcctgc ttcagcgctt cgagtctgga catcgccgag
     1561 tcccgcctgc cctcgcgaag atcgagcagc accagggttg cagcggccgc aaccgactgc
     1621 ggattcgcct gcgcgctctt ctgcagcgtt tcgcgcgcac tgccggtatt gccgagcgcc
     1681 aactgggctc gcgcaacatc catggcataa gacggattcc cggcatcctg gctgacggca
     1741 ttgcggaaga agttgagcgc ctcatcgaac ctgcccgcgt ccagataaat gcgtccgagc
     1801 gcctgactgg tcgccgcgtc cgcaccgcgg gactgaatct cgcgcacgat cgtatcggca
     1861 tctcgcgtct tcttctgctg catgtacagc cggatcagcg ccagtcgcgg ttccacggct
     1921 ttcggatctg cagcgcgcat ctgctcgagc tgctgcgtcg cgccggcagc gtcgccttcc
     1981 cgcaatgcga gttccaccag ggcgagtcgt ggggcggtat tggaagtgtc ga
//
LOCUS       DASSRL010000030         7540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1733092, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000030 DASSRL010000000
VERSION     DASSRL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733092"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFL49_00580"
     CDS             <1..746
                     /locus_tag="VFL49_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7910503.1"
                     /translation="VPVGKHLAGLAAKGMKKATMELGGHSPVVVFEDADPEKTADTIA
                     AFKYRNAGQVCISPTRFDVHEKNYSKFVARFTEYAKGLKLGDGLEKGITLGPLANPRR
                     LDAMEGFVNDAKDRGGKVVTGGSRHGNQGFFYQPTVITDVPDDSKIMTEEPFGPVAPI
                     VPFKTFDEVVERANSLQFGLAAYAFTTSDKNAAAISDALQSGMVGVNSVAISTPETPF
                     GGVKESGYGSEGGIEGLQAYMNIKFVSQA"
     gene            complement(763..3084)
                     /locus_tag="VFL49_00585"
     CDS             complement(763..3084)
                     /locus_tag="VFL49_00585"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7910504.1"
                     /translation="MTTTPARTRQTPRVEDNALVRGAGHFMDDPRLPNTVYAAFVRSP
                     HAHARVVAVRTDEARKAKKVLAVLTAQDMKAANVGTVSRHPPVPGRGGAKMVMPFRPS
                     LAGDKVMHVGDPVAMVVAETAAAALDAVELVQVDYEELPAVITLDDAMKGATQLYAEA
                     PGNLCVDWPGPIADEQNEHEVADAIASAPHVAKVRVTNQRMVVASMETRGATGVYDKA
                     SDSYTLYACSQGADSLRAQAAAIMGVPNEKLRVITKDVGGAFGMKTPVYPEYPALLIA
                     ARQLGRPVQWQSTRSEAFITDTQARDTVTEVELALDEKGKFLALRMRHLCNQGSYVST
                     AGVGINTNNFARCLPGMYRIPKIDASVACYFSNTIPIGPYRGAGRPEANYALERVVEE
                     AARVIGMDPVRLRRKNLIPPSAMPFKTPINTYDSGDFPAVVDKALELADFVNFNKRRR
                     ESARRRKLRGIGVSFMLEHAGALPMEEASVSFPAGDQLILGCNVQSTGQGHATVFPRL
                     LADKLGIEPERIQHRHGDTAQGLTGFASVGSRSAMCAGNAIVHTADVLLDKGKKVASA
                     LLEASEADIQYRNGNFEVVGTDRKISLFETARRAREIGESLDTKEKAETPLTFPNGCH
                     IAEVEVDPETGAVDLVTYTAVDDPGVMLDPIVVEGQVQGSIANGLGQALTENAIYDPQ
                     SGQLMAGSFMDYGMPRAHHMPIELREAVHSVPATSNPLGVKGTGEAGTTAAIAAVMNA
                     IANAIPNGAGDHMDMPATSAKVWEACQRGLAAK"
     gene            3224..4225
                     /locus_tag="VFL49_00590"
     CDS             3224..4225
                     /locus_tag="VFL49_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7910505.1"
                     /translation="MNNLRTIDTHTHILTEETAALLRKEAPKVSVTITPVDAEFATLD
                     VGGVVYRPFPRGGFDVAHRLRDMDAMGVDVQVLSATPQTYLYNQDASLGAATAAIQND
                     QIAKLVKENPQRFMGIATLPMQAPDKAAEELKRAMTKLGLRGTMFASNIMGKNLDDPS
                     FEPVWATAEELGAFVFIHPNNVAGADRMKSYYLNNLIGNPLDTTIGAACLYFGGVLDR
                     HPKLAVVLAHGGGFTPYQAARWEHGWHVRPEPKKNIARQPVDIARRFYYDTILHSAPV
                     LEFMIEHVGADRVMLGSDYPYDMGMMDCVKHVRGLKISDTDKATILNARAQALLGKT"
     gene            4222..5298
                     /locus_tag="VFL49_00595"
     CDS             4222..5298
                     /locus_tag="VFL49_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET7910506.1"
                     /translation="MNIQATKAARIPTKAVTGLIIDAMIKAHVPKDDAGKIAELMLEA
                     DLVGADAHGVFRLPQYVQRLKLGSTNARPNITVSRSAPATALVDGDNGMGHLVVARAA
                     ETAIELARECGVAWIGCRMSGHAGAAGVYAALPLNADMVGLYSAVANANHMPLAGGAE
                     PLLGTNPLAIAIPAGEEPPIVLDIATSIVSYGTIKNHRLQNKPLRNDWMIDPKTGAAV
                     VDPHKSVEALLLPMAGYKGAGLALTLGLLAGTLNGALFGRDCVDFNAEPDRVTNTGQF
                     VVALDPSRFQPLAQFKSEVDRHIREMRDTKPLPGHTVRLPGEQRAKRRADRLHNGLAL
                     PPELLAQLDNLAGELSIAPLRQRM"
     gene            5581..6714
                     /locus_tag="VFL49_00600"
     CDS             5581..6714
                     /locus_tag="VFL49_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7910507.1"
                     /translation="MTAGILLSGQTRGDWVELSAAGSWTAEYASAIEPQVEAATRGIT
                     ARGVAININRIEYLDTYGAWLIERAMRQWTREGREARLVGLREDFRGLFEKVHSGTQT
                     LSPPPARPNSIVATLVSVGMTMSAVAADLYLFVQMLGSLIAAFGRVILRPTSFRFTST
                     VHQLERVGWRAVPIVLLITVLIGAILAQQGIFHFRKFGADIYVIDMVGVLVLREVGVL
                     IVCIMVAGRSGSSYTAELGAMRMREEVDALRTMGFDPVEVLILPRIVALVIAVPILTF
                     LGSMAALYGGGLVAWLYGGIAPEVFLTRLREAISTTTFEVGIIKAPFMALVIGVVACV
                     EGLQVQGSAESLGEQTTNSVVKSIFLVIVLDGIFAMFFASIGM"
     gene            6716..7501
                     /locus_tag="VFL49_00605"
     CDS             6716..7501
                     /locus_tag="VFL49_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910508.1"
                     /translation="MAIDQRTPIIEVHDLVVDLNGHRILNGVTLDVYRGEILGFVGAS
                     GAGKSVLTRTIVGLLPKQQGRIDVFGVDLDQAGEAQRQEIERRWGILFQQGALFSSLT
                     VGENVEFPIREYLDVSDRLMTEIAITKLEMVGLRPDVYRKYPSELSGGMIKRVALARA
                     LALDPEILFLDEPTSGLDPISAGDFDQLILTLQRTSAMAVFMVTHDLDSLHTICDRIA
                     VLGEGKILEAGTMDTMLRSQHPWLKAYFHGARARGVLHAAARN"
BASE COUNT         1417 a         2313 c         2307 g         1503 t
ORIGIN      
        1 cggtgccggt cggcaagcat cttgccggcc tcgccgccaa gggcatgaag aaagcgacaa
       61 tggaactcgg cggacactcg ccggtggtcg tgttcgaaga tgccgatccc gagaaaacgg
      121 ccgataccat agcggcgttc aagtaccgca atgccggcca ggtctgcatc tcgccgacgc
      181 gattcgacgt gcacgaaaag aactacagca agttcgtggc gcgcttcacc gaatatgcca
      241 agggcctgaa acttggcgac ggcctggaga aaggcatcac gcttggcccg ctcgccaatc
      301 cgcgccggct cgatgcgatg gagggctttg tcaacgacgc caaggatcgt ggcggcaagg
      361 ttgtcaccgg cggttcgcgg cacggcaacc agggtttctt ctatcagccg accgtgatca
      421 ccgacgtacc ggacgattcc aagatcatga ccgaggagcc gttcggaccg gtggcgccga
      481 tcgtgccttt caagaccttc gacgaggttg tggaacgcgc caactcgctg cagttcgggc
      541 ttgccgccta tgccttcacg acgtcggaca agaatgcggc ggcaatcagc gatgcgcttc
      601 agtccggcat ggtcggcgtc aacagcgttg ccatctcgac gcccgagacg ccgttcggcg
      661 gtgtcaagga atccggctac ggctccgaag gcggcatcga gggcctgcag gcctacatga
      721 acatcaagtt cgtgtcgcaa gcatagagcg cgacggcaaa agctattttg cagcgagtcc
      781 ccgctggcag gcttcccata ctttcgcgga cgtcgccggc atgtccatgt gatcgcccgc
      841 cccgttgggg atagcgtttg cgatcgcatt catcaccgct gcgatcgccg cggtggtgcc
      901 cgcctcgccg gtgcccttca ccccgagcgg attgcttgtc gcgggcaccg aatgcaccgc
      961 ctcacgcagc tcgatcggca tatgatgcgc tcgcggcatg ccgtaatcca tgaacgagcc
     1021 ggccatcaat tgcccgctct gcggatcgta aatggcgttt tcggtcagcg cctgacccag
     1081 tccgttggcg atggagccct gcacctggcc ttctacgacg atggggtcaa gcatgacgcc
     1141 cggatcatca accgcggtat aggtgacgag atcgaccgcg ccggtttcgg gatccacttc
     1201 aacctcggcg atatgacaac cgttcgggaa agtgagcggc gtttcggcct tttccttggt
     1261 gtcgagactt tcgccgatct ccctggcgcg tcgggcagtc tcgaatagcg aaatcttgcg
     1321 gtcagtaccg acaacttcga agtttccgtt gcggtactga atgtcggctt ccgaggcttc
     1381 cagcaatgcg gacgcgactt ttttgccttt gtccagaagc acatcagccg tgtgcacgat
     1441 cgcgttgccc gcgcacatag ccgagcgcga accaaccgac gcaaaacctg tgagcccttg
     1501 cgccgtgtcg ccgtgcctgt gctgaatcct ttcaggctca ataccgagct tgtcagcgag
     1561 caagcggggg aagacggtgg catggccctg gccggtcgat tgcacattgc agcccaggat
     1621 caactggtcg ccagcgggaa atgaaactga agcctcctcc atcggcaacg ccccggcgtg
     1681 ctccagcatg aaggagacac cgatcccgcg cagcttccgg cgtcttgcgg actcgcgccg
     1741 gcgtttgttg aagttgacaa aatccgcgag ctccaatgcc ttgtcgacga ctgccgggaa
     1801 atccccgctg tcgtaggtat tgatcggtgt cttgaacggc atcgccgacg gcgggatcag
     1861 atttttcctg cgcagccgca ccggatccat cccgataacc cgtgcagctt cttccaccac
     1921 acgttcgagt gcgtagttcg cctccggccg tccggcaccc cgataagggc cgatcggaat
     1981 tgtgttgctg aagtagcagg caacggaagc gtcgatcttc gggattctat acatgcccgg
     2041 cagacagcgc gcgaaattgt tggtgttgat gccgacgccc gccgtgctca cataggagcc
     2101 ctggttgcag aggtgccgca tccgcagcgc cagaaacttg cctttctcgt caagcgcgag
     2161 ttccacctcg gtaaccgtgt cgcgcgcctg cgtgtcggtg atgaaagcct ccgatcgcgt
     2221 cgactgccac tgcacgggac ggccgagctg gcgggcggcg atcagcaagg cgggatattc
     2281 ggggtaaacc ggcgtcttca tgccgaacgc gccgccgaca tctttggtga taacgcgcag
     2341 cttctcgttg ggaactccca tgatggcggc agcctgcgca cgcagcgagt cggcgccctg
     2401 cgagcaggcg taaagcgtgt agctgtcgct cgccttgtca tagacgccgg ttgcgccgcg
     2461 cgtttccata gacgcgacca ccatgcgctg attggtgacg cgcacttttg ccacgtgcgg
     2521 cgcacttgcg atcgcgtcgg cgacttcatg ttcgttctgc tcgtctgcga ttggccccgg
     2581 ccagtcgaca caaagattgc cgggtgcctc ggcgtagagt tgcgtggcgc ccttcatggc
     2641 gtcgtcaagc gtgatgacgg ccggtaattc ctcatagtcg acctgcacca actcgaccgc
     2701 atcgagtgcc gctgccgcag tctcggcgac caccatcgcg accggatcgc cgacatgcat
     2761 caccttgtcg ccggccagcg acggacgaaa cggcatgacc atcttggcgc cgccgcgccc
     2821 cggcaccggc ggatgacgcg agacagtccc gacgttcgcc gccttcatgt cctgtgcggt
     2881 gagcacggcc agcacttttt ttgccttgcg cgcttcgtct gtcctcaccg caacgacgcg
     2941 cgcatgcgca tgtggcgaac gcacgaaagc tgcatagaca gtattgggca ggcgcggatc
     3001 gtccatgaag tgaccggcgc cgcgcaccag ggcattgtct tcgacgcgcg gcgtttgtct
     3061 ggtgcgtgca ggggtcgtag tcatgagaat tccggaaata gaatggcagg acccctgttt
     3121 atagcggaaa ggaccgcgcc cgacaggggc ttgcgtcgat gcgccggctg cggcactcag
     3181 gcgctccttg ccgcgtgtcg cgtggcgtct ttcgagtggt gaaatgaaca acctgcgaac
     3241 aatcgatacc catacccaca tccttaccga ggaaaccgct gccctgttgc gcaaggaagc
     3301 gcccaaggtt tctgtaacga tcacgcctgt cgatgctgag tttgcgacgc tcgacgtcgg
     3361 cggcgtcgtc taccgccctt ttccgcgcgg cggcttcgat gtagcgcatc gcttgcgtga
     3421 catggacgcg atgggtgtcg acgtgcaggt gctttcagcc acgccgcaga cctatctcta
     3481 taaccaggat gcttcgctcg gcgccgcgac ggcggctatc cagaacgatc agatcgcgaa
     3541 gctggtgaaa gaaaatccgc aacgcttcat gggtattgcg acgttgccga tgcaggcacc
     3601 ggacaaggct gccgaggagc ttaagcgtgc aatgaccaag ctcggcctgc gcggaacgat
     3661 gtttgcgtcc aacatcatgg gtaaaaatct cgacgatccg agtttcgagc cggtctgggc
     3721 gacggccgag gaactcggcg ctttcgtctt cattcatccg aacaatgtcg ccggcgcaga
     3781 ccggatgaag tcctactatc tgaacaatct tatcggcaat ccgcttgaca cgaccatcgg
     3841 cgctgcctgt ctttatttcg gcggcgtgct cgaccgacac cccaagctgg ccgtcgtcct
     3901 cgcgcacggc ggcgggttca cgccctatca ggcggcgcga tgggaacatg gctggcacgt
     3961 gcggccggag ccgaagaaaa atatcgccag gcagccggtc gacatcgcca gacgcttcta
     4021 ttacgacacg atcctgcatt cggcgccagt gctggaattc atgatcgagc atgtcggcgc
     4081 ggaccgtgtc atgctcggta gtgactatcc gtacgacatg ggcatgatgg actgcgtaaa
     4141 gcacgtgcgc ggcctgaaga tctcggacac cgacaaggcg acgatcttga acgcgcgcgc
     4201 tcaggctctg ttggggaaga catgaacatc caggcgacca aagctgcgcg cattccaaca
     4261 aaggccgtca caggtttgat catcgatgcg atgatcaagg cacatgtgcc caaggatgac
     4321 gccggcaaga tcgccgagtt gatgctggag gccgacctgg tcggcgccga tgcgcacggc
     4381 gtgtttcgcc tgccgcaata tgtacagcgc ctgaaactcg gctcaacaaa tgcgcgcccg
     4441 aacatcactg taagccgcag tgctccggca acggcgctgg tcgacggtga caacggcatg
     4501 ggccaccttg ttgttgctcg cgctgccgaa actgcgatcg aactcgcccg tgagtgcggc
     4561 gttgcgtgga tcggctgccg catgtccggg catgccggcg ccgccggcgt gtatgccgcc
     4621 ctgccgctga acgccgacat ggtcggactc tattcggccg tcgcgaacgc caaccacatg
     4681 ccgctcgccg gcggtgcgga gccgctgctc ggcacgaacc cgctggcgat tgcgataccg
     4741 gccggcgagg agccgccaat tgttctcgac attgcgacgt cgattgtctc ctacggcacc
     4801 atcaagaatc atcgtctgca gaacaagccg ctgcgcaacg actggatgat tgatcccaag
     4861 accggcgccg ccgttgtcga tccgcacaag agtgtagaag cgctgttgct gcccatggca
     4921 ggttacaagg gggcaggact tgccctcacg ctcggccttc tcgccggcac cctcaatggc
     4981 gcgctgttcg ggcgcgactg tgttgacttc aatgccgagc cggacagggt caccaacacc
     5041 ggtcagttcg tggtcgcgct cgatccaagc cgatttcagc cgctcgccca gttcaagtcg
     5101 gaggttgacc ggcacatccg cgaaatgcgc gacacaaagc cgctgcccgg ccatacggtc
     5161 aggctgcccg gcgaacagcg cgccaagcga cgcgcggatc ggcttcacaa cgggctggca
     5221 cttccccccg agttgcttgc gcaactcgac aatctcgccg gcgaactgtc gatcgcgcca
     5281 ctgcgccaac gcatgtgact ggtgacgtcg gtcaatttct cccgccggaa ggccggaagt
     5341 atagttaatc atgcattaac ctaattggtt aatcgccgtt taagaaatcc taccaatttg
     5401 ttaatttttg atggtaacgg ggcggggcga gatcgcgctc aatgcggccc tagcgatgcg
     5461 gcccgtgtcg atcagccgga tgtggccgcg aagtcccgcc gcaacgcatc cagtcctgtc
     5521 tcgtgtgccc gtcgcgcgct attcgcgcgc ggtgttgtgt cgtggatgga ggaacgttgc
     5581 gtgacggcgg gaattttgct cagcggccaa acacggggcg actgggtcga gctcagtgcc
     5641 gccggatcat ggactgccga atacgcaagc gcgattgagc ctcaggttga agcggctacg
     5701 cgcggcatca ccgcccgcgg cgtagcgatc aatatcaatc gcatcgaata tctcgatacc
     5761 tacggagcct ggctgatcga gcgcgcgatg cggcaatgga caagggaagg gcgcgaagcg
     5821 cgcctggtag ggttgcgtga ggattttcga gggctcttcg aaaaagtcca ttccggcacg
     5881 cagacactct caccgccgcc ggctcgcccc aacagtatcg tcgcaacgct ggtcagcgtc
     5941 ggcatgacaa tgtcggccgt cgccgccgac ctttacctgt tcgtccagat gctcggctcg
     6001 ctgatagccg ccttcggtcg cgtcattctg cggccgacga gcttccgctt cacgtcgacc
     6061 gtccaccagc tcgagcgagt cggatggcgg gcggtgccga ttgtcctcct gatcactgtt
     6121 ttgatcggtg cgattctcgc gcagcagggc atctttcatt tccgcaagtt cggagccgac
     6181 atttacgtca tcgacatggt cggcgtgctg gtgttgcgcg aagtcggcgt gcttattgtc
     6241 tgcatcatgg tcgcggggcg gtcggggagc tcttacacgg ccgagcttgg tgcaatgcgc
     6301 atgcgtgaag aggtggatgc gctgcgtaca atgggatttg accccgtcga ggtcctcatc
     6361 cttccccgca tcgtcgcgct ggtgatagcg gtccctatcc tcacgtttct cggctcaatg
     6421 gccgcgcttt acggtggcgg actggtcgcc tggctctacg gcggcatcgc gccggaggtt
     6481 ttcctgacgc gcctgcgcga ggcgatttcg accacaacct ttgaagtcgg catcatcaag
     6541 gcgccgttca tggcgctggt tatcggcgtg gttgcctgtg tcgaaggcct ccaggtgcag
     6601 ggtagcgcag aatctcttgg cgagcagacc accaattcag tggtcaaatc gatcttcctc
     6661 gtgatcgtgc tcgacggcat ttttgccatg ttcttcgcgt cgatcggaat gtgacatggc
     6721 tatcgatcag aggactccga tcattgaagt gcacgacctg gtcgtcgatc tgaacgggca
     6781 ccgaatcctc aatggcgtca cgctcgacgt ctaccggggc gaaatcctcg ggttcgtggg
     6841 tgcttcggga gcgggcaagt ctgtgcttac gcgcacaatt gtcgggcttt tgccgaaaca
     6901 gcagggccgc atcgatgttt tcggcgtcga cctcgatcag gccggcgagg cgcagcgcca
     6961 ggagatcgag cgccgctggg gcatattgtt tcagcagggg gcattgtttt cctcgctgac
     7021 ggtcggcgaa aacgtcgagt ttccgatccg cgagtatctg gatgtttcgg acaggctgat
     7081 gaccgaaatc gccatcacca agctggaaat ggtcggcctc aggcccgatg tctatcgtaa
     7141 atacccttcg gaactttccg gcggcatgat caagcgcgta gcgctcgcac gcgccctcgc
     7201 gctcgaccca gaaattttat ttctcgacga gccgacctcg ggcctcgacc cgatcagcgc
     7261 gggcgatttc gaccagctga tcctgacctt acaacggact tcggcaatgg ccgttttcat
     7321 ggtaacccat gacctcgaca gccttcacac gatctgcgac aggatcgcgg tccttggcga
     7381 aggcaagatt cttgaagccg gaacaatgga tacgatgctc aggtcgcagc atccgtggtt
     7441 gaaggcctat ttccatggcg cgcgcgcgag gggggttctc catgccgcgg ccaggaactg
     7501 acggagcgaa gtgatggaga gaaaagcgca ctatgcactg
//
LOCUS       DASSRL010000031         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_350948, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000031 DASSRL010000000
VERSION     DASSRL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350948"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFL49_00610"
     CDS             <1..182
                     /locus_tag="VFL49_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910509.1"
                     /translation="GGGLNRALSVYRLPEHPWDRHVRFEHAVTFSGGADLPVYVRVTQ
                     ADGNQAWSSPIYLIA"
     gene            complement(305..613)
                     /locus_tag="VFL49_00615"
     CDS             complement(305..613)
                     /locus_tag="VFL49_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7910510.1"
                     /translation="MAMVPGKAPTPPTVTLKHLAAALAEQHEMSKKQAETVLGDLVGN
                     IVKHLKKGERIRIGGLGILQVRKRAARIGRNPATGEQIQIKASKKVAFRASKELKESI
                     "
     gene            791..867
                     /locus_tag="VFL49_00620"
     tRNA            791..867
                     /locus_tag="VFL49_00620"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:825..827,aa:Asp,seq:gtc)
     gene            949..1650
                     /locus_tag="VFL49_00625"
     CDS             949..1650
                     /locus_tag="VFL49_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET7910511.1"
                     /translation="MTAVRAMFFDVFGTLVDWRTGVAREAERVLKPVGHDLDWLAFAD
                     AWRGEYQPGMEEVRSGRIPFCKLDVLHRRNLERFMPRFGLRNLSDAVLSELTLAWHRL
                     DAWPDVPAALSRLRKKFLLAPVSNGNISLMVDLARHNKFPWDAILGAEVAGDYKPKPD
                     VYLAACEAFDLKPEQCMMVAAHSSDLAAAAKCGLRTGHTARPDEYGPDTGERAPTVKV
                     DLAAKDLAGLADKLC"
     gene            complement(1750..>2127)
                     /locus_tag="VFL49_00630"
     CDS             complement(1750..>2127)
                     /locus_tag="VFL49_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7910512.1"
                     /translation="KAFIHSIFDRDHPGTAEEKVIDAFADAVVALDTSVPTGTYVDMC
                     SKLPVVNPEKIAVPTLIMRGQWDGIASIEDLLKFYAKLPNPDKQFTVMPGISHASFQQ
                     KNYMLVYHILASFFAQPAPVYRG"
BASE COUNT          385 a          615 c          663 g          464 t
ORIGIN      
        1 acggcggcgg tctcaaccga gcgctcagcg tgtaccggct cccggagcat ccctgggatc
       61 ggcatgtcag atttgagcac gctgtgacgt tcagcggcgg agctgatctg cctgtgtacg
      121 tgcgtgtcac ccaggccgac ggcaatcagg cgtggtcaag tccaatctac ctgattgcat
      181 gagcgctgcc tttgtcatgc tggaatggtt ggtgcattca cggtgcaaag acaaacgaaa
      241 cggcaggcgg gagcctatcg ctccacgcct gccgaatttc cccggagggt caggcaggaa
      301 gtcgtcagat cgactctttc aactcttttg acgcgcggaa ggcgaccttc ttgctcgcct
      361 tgatctgaat ttgctcgccg gtcgcaggat tgcggccaat gcgtgctgcg cgcttacgaa
      421 cctgaaggat accgagaccg ccgatgcgga tgcgctcgcc ctttttcaga tgcttcacga
      481 tattgccgac gagatcgccc agcacggttt ccgcctgctt cttcgacatc tcgtgttgtt
      541 ccgcgagagc cgcagcaaga tgcttcagcg tgacggtcgg tggtgtcgga gcttttccag
      601 gaaccattgc catcagtggg accctcctct tgaatccgcg agcttggcga agcgccaaac
      661 acaccgcccg aatagacgat tcgggggcgg tctgaccatc gattcttctc ggtaaataag
      721 gctttttcgc aatttttggt gtttaccttg actatgcatt gagccgccta taactcgtcc
      781 ggacgcccgc gggggcgtag ctcagttggt tagagcgctg ccctgtcacg gcagaggccg
      841 cgggttcgag tcccgtcgct cccgccattt tatcaatgac ttagctgggg ttttgggctg
      901 cctcgccggc gctcgtcgag gccggcaaac ggctccgcgg gtactgtcat gacagccgtt
      961 cgggcgatgt tctttgacgt gttcgggact ctggtcgact ggcggaccgg ggtcgcccgg
     1021 gaagcggagc gggtgctcaa gcccgtcggg catgaccttg actggttggc ctttgcggac
     1081 gcctggcggg gcgaatacca gccgggaatg gaggaagtcc gttccggccg catcccgttt
     1141 tgcaagctgg atgtgttgca tcggcgcaat ctcgagcgct tcatgccgcg ctttggcttg
     1201 cgcaatttgt ccgacgctgt tctgagcgaa ctcacgcttg cttggcatcg gctcgacgcc
     1261 tggccagatg tgccggcggc gctcagtcgc ctgcgaaaga aatttctgct tgcgcccgtg
     1321 tcgaacggca atatttcgtt gatggtcgat ctcgcgcggc acaacaaatt tccctgggac
     1381 gccatcctcg gcgccgaggt cgccggcgac tacaagccga agccggatgt ctatcttgcc
     1441 gcctgcgagg cgttcgacct caagccggag cagtgcatga tggtggccgc gcactcgagc
     1501 gatcttgctg cggcggcgaa atgcgggttg cgcaccggcc ataccgcgcg gcccgacgag
     1561 tacgggcccg acacaggcga gcgcgcgccg acggtgaaag tcgatttggc cgcgaaggat
     1621 ttggcgggct tggcagacaa gctttgctag caaattcgtt atggccgggc ttgtcccggc
     1681 catccacgtt ttcattctca aagagacgtg gatgcccggc acgaggccgg gcatgacgaa
     1741 tgagaggcct cagcccctat aaaccggtgc cggttgcgcg aagaagctcg ccaatatgtg
     1801 gtagaccagc atgtagttct tttgctggaa gctggcgtgt gaaatgcccg gcatcactgt
     1861 gaactgcttg tcgggattcg gcagcttcgc atagaacttc agcaaatcct caatcgaagc
     1921 aattccgtcc cactggccgc gcatgatcag cgtcgggacg gcgatcttct ccggattcac
     1981 cacgggcaat ttcgagcaca tatcaacgta ggtgccggtc gggacggatg tgtcgagtgc
     2041 gaccaccgca tcggcgaaag catcgatgac cttttcttcg gcagtgccgg gatgatcgcg
     2101 gtcgaagatc gagtgaatga aggcctt
//
LOCUS       DASSRL010000032         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_893438, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000032 DASSRL010000000
VERSION     DASSRL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893438"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFL49_00635"
     CDS             <1..713
                     /locus_tag="VFL49_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910513.1"
                     /translation="DRPYTEDKQDVVQGACLWGTICNASAGMGRVVTEGYGDSPWAIF
                     VRQDSRIRKPEDLKDVPVSVGMRAGSHFNVPYRLEKYLPLEHIKTVNTGGFGARLKAL
                     LDGEVEAASLLPPQIAMAEQLGLRKIIEDRFKTLWWVPDSATPDVVHGYMRALNRAEQ
                     AMDADLPKYLPLWKLAVPAEFETYHSWDFSKFGRGERFMNKPLPRGEFGEVFAQVERW
                     GLDQFLKDRSPETLSYQT"
     gene            complement(770..2011)
                     /locus_tag="VFL49_00640"
     CDS             complement(770..2011)
                     /locus_tag="VFL49_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910514.1"
                     /translation="MTLRSNRRQFLAGSAAAAGAAAFPMPAIAQNAPMKVGLLTVKTG
                     PLAAGGQHAEEGITSFLKDKNFTLSGRKIELLVADTGGSPAGAKTKAQELVERDKVNV
                     VLGPFAAFELLATLDYFAQAKMPTLAFAGAEDVTQRKGNPYLTRTSYTSAQCLYPLAD
                     YMQSEMKLKTAATIADDFAFGYEQVGGFQRVLEEEGGRVVKKLWSPLNTADYAPYVAQ
                     IPECDAVCVGLAGSNPIKFIKQAKGLGIKQQIVGGSTTADDTIIQAYGDEAQGLINTN
                     PYSLDIDSEANKRFIAAMRKNYGADVRIGHYAACFYIDGQVIEAALTKTAGKSDNPDE
                     FVKAVRSITLADTPRGPISFDDYGNVVIDVYVRRAEKQGDKMVNKTIKTYHKVSQFWR
                     MDPTKFLSQPVFSRDFPPMKG"
     gene            complement(2148..>2756)
                     /locus_tag="VFL49_00645"
     CDS             complement(2148..>2756)
                     /locus_tag="VFL49_00645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197125.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the delta' subunit seems to
                     interact with the gamma subunit to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7910515.1"
                     /translation="IVDAVDDLQREGANALLKILEEPPQRSLILLVSHAPGRELPTIR
                     SRCRRLLLRPLEVDDVTLALAEATGKDRRDDDVRETAAAAEGSVGRALSLLEGQTLAL
                     RQRVLDLLEQLPNVDTRALHALGDAMGGTDPKVLEAFMDLINGWLSARLGEHAQETSR
                     MARIAETWERVNSAARDVDTYNLERKPLVFSVFGALADAARP"
BASE COUNT          495 a          870 c          859 g          532 t
ORIGIN      
        1 aggaccgccc ctacacagag gacaaacagg acgtcgtgca aggcgcatgc ctgtggggca
       61 cgatctgcaa tgcgagtgcc ggcatgggcc gcgtcgtcac cgaaggttat ggcgattcac
      121 cctgggcgat ctttgtgcgg caagattcca ggatccgcaa gccggaagac ctgaaggacg
      181 tgccggtctc cgtcggcatg cgcgccggca gccatttcaa cgtgccctat cgcctcgaga
      241 aataccttcc gctcgagcac atcaagaccg tcaataccgg cggcttcggt gcgcggctca
      301 aggcgctgct tgacggcgag gttgaagcag cgagtctgct gccgccacaa atcgcgatgg
      361 ccgagcaact cgggcttcgc aagatcatcg aggatcgctt caagaccctg tggtgggtgc
      421 cggacagcgc cacgccggac gtcgtgcacg gctacatgcg cgcgctcaat cgcgccgagc
      481 aggcgatgga cgcggacctg ccgaaatacc tgccgctctg gaaactcgcg gtccctgccg
      541 aattcgagac ctaccactcg tgggacttca gcaagttcgg ccgcggcgag cgctttatga
      601 acaaaccgct gccgcgcggg gagttcggcg aagtcttcgc gcaggtcgag cgctggggtc
      661 tcgaccagtt cctcaaggat cgcagccccg agacgctgtc ctaccagacc tgaacaatgt
      721 ccctggcaca ggacaaagcg cgcgacctca ggccgcgcgc ttttttcgtt cagcccttca
      781 tcggcggaaa atcgcgcgag aacaccggct ggctgaggaa tttggtcggg tccatcctcc
      841 agaactggct gaccttgtga taggtcttga tggtcttgtt gaccatcttg tcgccttgct
      901 tctcggcgcg ccgcacataa acgtcgatca ccacgttgcc gtagtcatcg aagctgatcg
      961 gcccgcgcgg cgtgtccgcg agcgtgatgg agcggaccgc cttcacgaat tcgtccgggt
     1021 tgtcggattt gccggcggtc ttggtgaggg cggcttctat gacctgcccg tcgatataga
     1081 agcacgccgc gtagtgaccg atgcgcacat ccgcgccgta gttctttcgc atcgccgcga
     1141 tgaaccgctt gttcgcctcg ctgtcgatat ccaatgaata gggattggtg ttgatcagcc
     1201 cctgcgcctc gtcgccgtaa gcctgaatga tggtgtcgtc ggctgtggtc gagccgccga
     1261 cgatctgctg ctttatgccg agcccctttg cctgcttgat gaacttgatt ggattcgaac
     1321 ccgccaatcc aacgcaaact gcatcacatt ccggaatctg cgcgacatag ggcgcgtagt
     1381 cggcggtatt gagcggcgac cacagcttct tgacgacgcg tccgccctcc tcctcgagca
     1441 cgcgctggaa gccgccgacc tgctcgtagc cgaatgcgaa gtcatcggcg atcgtggccg
     1501 cggtcttgag cttcatttcc gattgcatgt agtccgcaag cggatagaga cattgcgccg
     1561 aggtatagga cgtgcgggtg agataggggt tgcccttgcg ttgggtcacg tcctcggcgc
     1621 cggcaaaggc aagcgtcggc atcttcgcct gcgcgaaata gtcgagtgtt gccaggagct
     1681 cgaacgccgc gaacgggcca agcacgacat tgaccttgtc gcgctcgacg agttcctgcg
     1741 ctttcgtctt tgcgccggca ggcgagccgc cggtatcggc gaccaggagc tcgatcttgc
     1801 ggccggacag cgtgaagttt ttgtccttga gaaacgacgt gatgccttcc tcggcgtgct
     1861 gaccgccggc agcgagcggg ccggtcttta cagtcaacaa tccgaccttc atcggcgcgt
     1921 tttgagcgat ggcgggcatc ggaaaggcgg cggcgccggc cgcagctgcc gaaccggcaa
     1981 gaaattgccg gcggttcgat ctgagagtca tggcatcctc ccctgggctt cggccgttct
     2041 ggcgcggccg gatattctgg acgtgatgcg aatgccgcaa attcaatcac aaggcggcgt
     2101 ccaggcgaac ccctttgcgg cccacttcgc ccacgcgaag aaaccgccta tggccgggcc
     2161 gcatccgcga gcgcgccaaa gacactaaag accagcggct tgcgttcgag gttgtaggta
     2221 tccacgtcgc gtgccgcgct gttgacacgc tcccacgtct cggcaatccg cgccatccgc
     2281 gacgtttcct gcgcatgctc gccgagtcgc gctgaaagcc agccgttgat cagatccatg
     2341 aaggcttcga gcaccttcgg gtcggtcccg cccatcgcgt cgccgagcgc atgcaaggcg
     2401 cgcgtatcga cgttcggcaa ctgctccagc aggtcgagta cgcgctgacg caatgccagc
     2461 gtctgtcctt cgagcaaact gagcgcccgc ccgacgctcc cctcggccgc cgctgccgtc
     2521 tcgcggacat cgtcgtcgcg gcggtctttt ccggttgcct cagcaagcgc caacgtaacg
     2581 tcgtcgactt cgaggggtcg gagcaggagc cgccggcacc gcgagcggat ggtcggcaat
     2641 tcacggcccg gagcgtggct gacgagcaga atgagcgatc gctgaggcgg ctcttccaga
     2701 atcttcagga gcgcattggc gccttcgcgt tgcaggtcgt cgacggcatc gacaat
//
LOCUS       DASSRL010000033         8964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1733146, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000033 DASSRL010000000
VERSION     DASSRL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733146"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFL49_00650"
     CDS             complement(<1..376)
                     /locus_tag="VFL49_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910516.1"
                     /translation="MRSTIKLIAAGVTLSMVALGIWPAAAAGYPDHPVKIVVPFAPAG
                     PTDVMARLIAQKLSESLKQQFYVENHPGAGGNIGMTQVAKSPPDGYTILVASSSYVVN
                     PSLYAKNPYDPFKDFAPVTLAAA"
     gene            502..1473
                     /locus_tag="VFL49_00655"
     CDS             502..1473
                     /locus_tag="VFL49_00655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7910517.1"
                     /translation="MPSRKITRQDMRDAAEKYKNWGKWGPNDEIGTLNYTTAEDIVAA
                     TRLVKKGKVISLALNFDHTGPQGAKSKYPSMGRTNPIHTMLRTGTDAYSGVLDKRGIR
                     AADDMVTMPLQCGTQWDGLGHVFYEDYMWNGYDCREVTSAGAQKCGIEKTKNKMVGRG
                     VFLDVARYKGVESLDDGYGITNDDLYNTAKKQKLELKRGDYVIVRTGMMERCQKAASW
                     DGYPGGDAPGFSFETLDFVKETHLAALASDTWGCEVRPNESEEGINQPWHWITIPIMG
                     MTMGEIFQLKELADDCEQDKVYEFLFVAPAIPITGAVGSPVNPLAIK"
     gene            1497..1895
                     /gene="msrB"
                     /locus_tag="VFL49_00660"
     CDS             1497..1895
                     /gene="msrB"
                     /locus_tag="VFL49_00660"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7910518.1"
                     /translation="MAKEYPVTHTDAEWRKLLTPEQYQIMRNHGTEMPGSCALLHEKR
                     AGTFRCAACGQPLFVSKVKFESGTGWPSFNDPVAGAVETDTDRSHGMVRDEVHCSRCG
                     SHLGHVFDDGPPPTHLRYCINGVALKFEPA"
     gene            1898..3592
                     /locus_tag="VFL49_00665"
     CDS             1898..3592
                     /locus_tag="VFL49_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7910519.1"
                     /translation="MATVGHNARRVGAEVLADMLAAYGVTHVFMVPAVLRRTFAEMER
                     RTNIARIHCHGEKSAAYMADGYARASGKPGVCMAQVIGALNLAAGLRDAYLAHSPVIA
                     MTGGRDPKTKFRKVYQEIDDVPAFEPVTKMNVTIDDVARIPDMVRQAFRTATTGAPGP
                     VHLQFRGNEGQVDLEEAEMDGLVEAQFSRVPPFRPEPEAAHVKAALELLQKAERPVIV
                     AGGGARASGAGKELVALAEALQIPIATSLNGKDIIPGIHPLSVGVVGSYSRESANRAV
                     NAADLVCFIGTETGGMTTHFWAVPKIGTPAIQIDLEPEALGRNYPLQASVLGDAKSVL
                     VRMLGQIDKSTATRRAAWIKEIGSLRSDWYAKYQPLLESDAVPIHPARICGELSKHMP
                     DDAIVVVDTGHAGMWMGGMFDLRTPAQSYMRSAGHLGWAFPAGIGAKAACPERPVVVF
                     TGDAGFWYHIGEVETAVRWNIASVTIVNNNGGGNQSKRGFDRAYGGEQTAQARELWTY
                     NKMNFARLAEDMGALGIRVEDAKDIGPALERALSAKRPAVIDIATDIEALAPTAVT"
     gene            complement(3598..4689)
                     /locus_tag="VFL49_00670"
     CDS             complement(3598..4689)
                     /locus_tag="VFL49_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910520.1"
                     /translation="MKLASSFAVFALSIAPALADDLPKFQVDASWPKTLPNNWIMGQA
                     AGVAVDSQDHIWVIQRPRTLTDDEKAATFNPPRTKCCVPAPPVLEFDQDGNLLKAWGG
                     PAHGYDWFENEHGINVDHKGFVWVGGNGSNDGHVLKFTQDGKFVMQIDMPGPQTGSAD
                     TTRLGRPADTEVDPATNEIYIADGYGNHRVIVFDADTGAYKRHWGAYGRPPGDAKAPA
                     YNPAEPPSQQFANPVHCVKIAKDGLVYVCDRSNDRIQVFRKDGTFVKEFLVAKQTLQQ
                     GSVWDLDFWPDARQTFLMNADGANNEVRTLVRETGEVVGAFGRNGRNAGEFHWIHNLA
                     VDSKGNIYTTEVDTGKRAQKFRYVGPPLK"
     gene            4835..5305
                     /locus_tag="VFL49_00675"
     CDS             4835..5305
                     /locus_tag="VFL49_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7910521.1"
                     /translation="MKRATLYICGLLTGLVLAFAIPGGQAHDADVLASSAPQKNPLFI
                     NMTSGDSWRGWMGLHFAHATLKQGHPVAVFLNLDAVRLAAKSGEQEKRPSMQRVPREI
                     LSDFIKDGGIVLLCGPCMIEFGLKLEDLIPGVQMGKPGYTQSFIFAENARTLTW"
     gene            complement(5311..6483)
                     /locus_tag="VFL49_00680"
     CDS             complement(5311..6483)
                     /locus_tag="VFL49_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET7910522.1"
                     /translation="MPKPLAGLRVLELARILAGPWAGQVLADLGADVIKVERKGSGDD
                     TRGWGPPFVEGTDGNHIGSAYFHAANRGKRSIEMDFESDEGRRIVRKLAARSDVLIEN
                     FKVGGLAKFGLDYKSLAPECPRLIYCSVTGFGQEGPYAKRAGYDLMAQGMGGIMDLTG
                     MADGEPTRIGIPVSDIFTGVYSVVGILAALAEREKTGKGCYVDTALVDSTVGVLSNQA
                     LNYLVSGKVPKRIGNAHANIVPYQVFPVADGHIIVATGNDNQYVKFCNVIGGAELAQN
                     PDYKDNVGRLKHRSELVAKLSALTARMKRDDLLAKLEAQAVPAGPINDLEQVFNDAQV
                     KHRGMKLSLPSKAAKAGMIPGVRTPIVIDGWRAASERPSPLLGEHTAEILREIGEG"
     gene            6734..7729
                     /gene="dctP"
                     /locus_tag="VFL49_00685"
     CDS             6734..7729
                     /gene="dctP"
                     /locus_tag="VFL49_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET7910523.1"
                     /translation="MRRFSALLATAAATILITASAQAQVILKASHQFPGGKGDPRDEM
                     VQIIAKEAKAANVGLEVQVFPGASLFKPNDQWNAMVNGQLDISSFPLDYASGKVRAFG
                     ATLMPGLVRSHDRAARMKNSEFMKDIKAKIEKAGVVVLADAWLAGAVASKKACIRKPD
                     DMKGLKIRSAGPTFAAMWQEAGASIVSIPSNEVYNALQTGVADATDTSTGSFVSFRIY
                     EQVKCITAPGENALWFMYEPVLMSKKSFDKLDKKQQDALIAAGKKSEEYFAKESKALD
                     DKMVKAFKDHNVEVVSMTPAEYQAWLDVAKKSSYAHFAKEVPDGKKLIDEALAVK"
     gene            7818..8384
                     /locus_tag="VFL49_00690"
     CDS             7818..8384
                     /locus_tag="VFL49_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET7910524.1"
                     /translation="MDFFILTVKRISQICGVIAAGLIAAGVLVVCEMVFVRYVLNQNT
                     IWETDFTTYCLVGATFIGSPYVLLTRGHVNVDVLPHYLGQEGRYWLALASILMSLAFC
                     LIMTVLTFNFWHEAWTQNWVSDTMWRARLWIPYSTMPIGLGLITLQYVVDLIALLTGR
                     EPPFGLSPRETAEDAARSQARQALGDVP"
     gene            8381..>8964
                     /locus_tag="VFL49_00695"
     CDS             8381..>8964
                     /locus_tag="VFL49_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7910525.1"
                     /translation="MSPTTQGAIVLVVTLIVLLSGAPVAFGLGAIAIAFLVIFQGFDS
                     LHVAAETFYTALQDFTLVSIPMFVMMGAAIGSSPAGKDLYEALDRWLYRVPGGLVISN
                     LGACAIFAALTGSSPACCAAIGKMGIPEMRSRGYPDDVATGSICAGGTLGILIPPSIT
                     FILYGIATETSIGRLFLAGVVPGLMLTGLFILWT"
BASE COUNT         1724 a         2697 c         2808 g         1735 t
ORIGIN      
        1 aagccgcggc gagcgttacc ggcgcaaagt ccttgaacgg gtcgtacggg ttcttggcgt
       61 aaaggctggg attgaccacg tagctcgagc tcgcgaccag gatcgtgtag ccgtccggcg
      121 gtgatttcgc gacctgcgtc atcccgatgt taccgccggc accgggatga ttttcgacgt
      181 agaactgttg cttcaggctt tccgacagct tctgcgcgat caggcgcgcc atcacgtcgg
      241 tcggtccggc gggggcgaac ggcaccacga ttttgaccgg atggtccgga tagccagcgg
      301 cggcggccgg ccagatgccg agcgcaacca tggacagcgt cacaccggcg gcgatgagtt
      361 ttattgttga gcgcattcgt ctcctcccaa tttttttcat cgtagcattg cacgggaacg
      421 ttgccagtgg ggcttgcatc gcccgcgcgc acagggtgta ttccggcggc ttgtcgttcc
      481 attttcgtca gaggaaattc aatgccgtcc cgaaagatca cccgccagga catgcgcgat
      541 gccgcggaga agtacaagaa ctggggcaaa tggggcccca atgatgaaat cggaacgctc
      601 aattatacga ctgccgaaga catcgttgcg gcgacgcggc tcgtgaagaa aggcaaagtc
      661 atctcgctcg cgctcaattt cgaccacacc gggccgcagg gcgcgaagag caaatatccg
      721 tcgatgggcc gcacgaatcc gatccacacc atgctgagga cagggaccga cgcctattcc
      781 ggcgtgctcg acaagcgcgg catccgcgcc gccgacgaca tggttacgat gccgctccag
      841 tgcggcacgc aatgggacgg ccttggccac gttttttacg aggactatat gtggaacggc
      901 tacgactgcc gcgaggtgac gagcgccggc gcgcagaagt gcggaatcga aaagacgaag
      961 aacaaaatgg tcggccgcgg tgtttttctt gatgtcgccc gctacaaggg cgtcgagtca
     1021 ctcgacgacg gctacggcat caccaatgac gatctctaca acacggcgaa gaaacagaaa
     1081 ctcgagctca agcgcggcga ctacgtcatc gtgcgcaccg gcatgatgga gcgctgccag
     1141 aaggcggcca gctgggacgg gtatccgggc ggcgacgcgc ccggcttttc attcgagact
     1201 ctcgatttcg tgaaggaaac gcatcttgcg gcgcttgcga gcgatacctg gggatgcgaa
     1261 gtgcgtccga atgagagcga ggaggggatc aatcagcctt ggcactggat cacgatcccg
     1321 atcatgggca tgaccatggg cgagattttc cagctcaagg aacttgccga cgactgcgag
     1381 caggacaagg tctatgagtt tctgttcgtt gctccggcca taccgatcac gggtgcggtg
     1441 gggtcgccgg tcaacccgct ggcgataaaa taagaggcgt cggaggagtt ctggtcatgg
     1501 cgaaggaata tccggtcacg cataccgacg ccgaatggcg caagctgctc acgcccgagc
     1561 agtaccagat catgcgcaac cacggcaccg agatgccggg aagttgcgcg ctgctgcatg
     1621 aaaagcgcgc cggcacgttc cgctgcgcgg cctgtggtca gccgttgttt gtctccaagg
     1681 taaaattcga gagcggaacg ggctggccaa gctttaatga tcccgtcgcc ggggccgtcg
     1741 aaaccgacac cgaccgcagc cacggaatgg tccgcgacga agtgcattgt agccgctgcg
     1801 gaagccacct cggtcacgta ttcgatgacg ggccgccgcc gacgcatttg cgctattgca
     1861 tcaacggcgt tgcgctgaag ttcgagccgg cctgagcatg gcgacggtcg gacacaacgc
     1921 caggcgcgtc ggtgccgaag tgcttgccga catgctggcc gcctacggcg tcacacatgt
     1981 tttcatggtg cctgccgttc tccgccgcac cttcgccgaa atggagcgcc ggaccaacat
     2041 cgcgcgcatc cattgtcatg gcgagaaatc cgccgcctac atggccgacg gctatgcgcg
     2101 cgcgtcgggc aagcccggcg tctgcatggc gcaagtcatc ggcgcgctca atctcgccgc
     2161 gggcttgcgc gatgcctacc tggcacactc gccggtcatc gcgatgacgg gtgggcgcga
     2221 tccaaaaacg aaattccgca aggtctatca ggaaatcgac gacgtgccgg cattcgagcc
     2281 ggtgacaaaa atgaacgtca ccatcgacga cgtggcgcgg atccccgaca tggtgcggca
     2341 ggcgtttcgt acggcgacca ccggcgcgcc gggccctgtg cacctgcaat tccgcggtaa
     2401 cgaggggcaa gttgatctcg aggaagccga aatggacgga ctcgtcgagg cgcagttttc
     2461 acgcgtgccg ccgttccgtc ccgagccgga agccgcgcat gtaaaggcag cgctcgaact
     2521 gctgcagaag gcggagcggc cggtcattgt cgcaggcggc ggcgcccgcg cctccggcgc
     2581 cggcaaggaa ctcgttgctc tcgccgaagc gctgcagatt ccgattgcga cctcgctcaa
     2641 tggcaaggac atcattcccg gaattcaccc attatctgtc ggcgtggttg gcagttattc
     2701 gcgcgaaagc gccaaccgcg cggtgaatgc ggccgatctc gtctgcttca tcggcaccga
     2761 aaccggcggc atgacgacgc acttctgggc ggtgccgaaa atcggcacgc cggcgatcca
     2821 gatcgacctc gagccggaag cgctcggccg taactatccg ctgcaggcaa gcgtgctggg
     2881 cgatgccaag agcgtgcttg tgcgcatgct ggggcagatc gacaagtcga ccgccacgcg
     2941 ccgcgcggcc tggatcaagg aaatcggttc gctacggagc gactggtatg cgaagtatca
     3001 accgctgctg gaatccgacg cagtgccgat tcaccctgcg cgcatctgcg gcgaactgag
     3061 caagcacatg cccgacgacg ccatcgtcgt cgtcgatacc ggtcacgccg gaatgtggat
     3121 gggcggcatg ttcgacctgc gcacgccggc gcagagctac atgcgcagcg ccggacacct
     3181 cggctgggcc ttcccggccg gcatcggcgc gaaagccgcg tgccccgaac ggcccgtcgt
     3241 tgttttcacc ggcgacgccg gcttctggta tcacatcggc gaagtcgaga cggccgtgcg
     3301 ctggaacatc gcctcggtca ccatcgtcaa caacaatggc ggcggcaatc agtccaagcg
     3361 cggctttgat cgcgcttacg gcggcgagca gaccgcgcag gcgcgcgaat tatggaccta
     3421 caacaagatg aattttgcgc gattggctga ggatatgggc gccctcggca tccgcgtcga
     3481 ggatgcaaag gacatcgggc cggcgcttga gcgcgcgctc tccgccaaac gtccggcagt
     3541 gatcgacatc gccaccgata tcgaagcact ggcgccgacg gcggtaactt aacgcctcta
     3601 ttttagcggc ggaccgacat agcggaattt ctgcgcgcgc ttcccggtgt cgacttccgt
     3661 cgtgtagatg ttgcctttgc tgtccactgc gagattgtgg atccagtgga attcgccggc
     3721 attgcggccg ttgcggccga aagctccgac gacttctccg gtttcccgca ccagcgtgcg
     3781 cacctcgtta ttggcgccat cggcattcat caggaatgtc tgcctggcat ccggccagaa
     3841 atcgagatcc caaaccgaac cctgttgcag cgtctgcttc gcgaccagga attccttcac
     3901 gaaggtcccg tctttacgga agacctggat gcggtcattg gaccggtcgc aaacatagac
     3961 gagtccgtct ttcgcgatct tcacgcagtg aactgggttt gcgaactgct gtgatggcgg
     4021 ctcggctgga ttgtaggcgg gcgccttggc atcgcccggc ggcctgccgt aggcgcccca
     4081 atgccgcttg tacgcgccgg tgtcggcgtc aaatacgatg acgcggtgat tgccgtaacc
     4141 gtccgcgatg tagatctcgt tcgttgccgg atcgacttcg gtatcggcgg gacgaccgag
     4201 gcgcgtggta tcggcactgc cggtctgcgg gcccggcatg tcgatctgca tcacgaattt
     4261 cccatcctgc gtgaatttca gcacgtgacc gtcgttgctg ccgttgccgc cgacccagac
     4321 gaagcctttg tgatcgacat tgatgccatg ctcgttctcg aaccagtcgt aaccgtgtgc
     4381 ggggccgccc catgccttga gcaggttgcc gtcctggtcg aattcaagaa ccggcggtgc
     4441 gggcacgcag catttggtgc gcgggggatt gaacgtcgcc gccttctcgt cgtcggtaag
     4501 cgtgcgcggc cgttggatca cccagatatg atcctgcgaa tcgacagcga cgcccgcggc
     4561 ctgccccatg atccaattgt tcggcaaggt tttcggccag gacgcgtcga cttggaattt
     4621 cggcaaatca tcagcgagcg cgggtgcaat cgacagcgcg aacacggcaa acgatgaagc
     4681 taatttcatg agcgtctccc ccggccagcg gccaggcgct ggcatcgcac cgatggtcca
     4741 ccttcgggct gccgcgttca agagtggagc ggtatggccc ggtacgatcc tgcgatagta
     4801 tcggtatgga tttgcctttc cggagggtga cacgatgaaa cgggcgaccc tttacatatg
     4861 cggtctcttg accggcctgg tcttggcctt cgcgattccc gggggacagg cgcacgatgc
     4921 cgatgtgctc gcaagcagcg cgccgcagaa gaacccgctg tttatcaata tgacgagtgg
     4981 tgattcatgg cgtggctgga tgggtttgca cttcgctcat gcgacgctga agcaggggca
     5041 cccggttgcc gtttttctca acctcgacgc ggtcaggctg gcggcaaaaa gcggcgagca
     5101 ggagaaaagg ccgtccatgc agcgtgtgcc gcgtgaaatc ctgtccgact tcatcaagga
     5161 tggcggtatc gtgctgctgt gcggcccgtg catgatcgag ttcggcctga aactcgaaga
     5221 cctgataccc ggcgtgcaga tgggcaagcc gggttacacg cagagcttta ttttcgcgga
     5281 aaacgcacgc acgctgacct ggtagcgcgc tcagccctcg ccgatctcgc gcaggatttc
     5341 cgccgtgtgc tcgccaagca ggggcgaggg acgctcggaa gcagcccgcc agccgtcgat
     5401 cacgatcggc gtgcgcacgc cgggaatcat cccggccttg gccgccttgc tcggcagact
     5461 gagtttcatg ccccggtgtt tgacctgcgc gtcattgaac acctgctcga gatcgttgat
     5521 cgggcccgcc ggcaccgcct gagcttcgag ttttgcgaga agatcgtcgc gcttcatcct
     5581 ggcggtaagc gcagacagtt tggcgaccag ctcgctgcgg tgcttgagac gtccgacgtt
     5641 gtctttgtag tccggatttt gcgcgagctc agcgccgccg atgacattgc agaacttgac
     5701 gtactggttg tcgttgccgg tcgcgacaat gatgtggcca tccgcaacgg gaaatacctg
     5761 gtagggcacg atgttggcat gcgcgttgcc gatgcgcttc ggcaccttgc ccgaaacgag
     5821 atagttcaaa gcctggttcg acaatacgcc gacagtcgag tcgaccaggg ctgtatcgac
     5881 gtagcaacct ttgcccgtct tctcgcgttc ggccagcgct gccaaaatcc cgacgacgga
     5941 atagacaccg gtgaagatgt cggagaccgg aatgccgatg cgggtcggct cgccgtcggc
     6001 catgccggtc agatccatga tgccgcccat gccctgcgcc atcaggtcgt agccggcgcg
     6061 tttggcgtag gggccttcct gaccgaagcc ggtgacggag cagtaaatca ggcgcgggca
     6121 ctcgggcgcg aggctcttgt agtcgaggcc gaacttggcg agaccgccga ccttgaaatt
     6181 ctcgatcagc acgtccgagc gcgccgccag cttgcgtacg atgcgccggc cctcgtcact
     6241 ttcgaaatcc atttcgatcg agcgcttgcc ccggttggcc gcatgaaaat aagcagaacc
     6301 aatgtgattg ccgtcggtgc cttcgacgaa aggcgggccc cagccacggg tatcgtcgcc
     6361 ggagcctttg cgctcgacct tgatcacgtc ggctccgaga tcggcgagca cctgtccggc
     6421 ccaaggcccg gccaggatac gggcaagttc gagtacgcgg aggcccgcta acggcttcgg
     6481 catgtaaaga agctcccagg gaagaaggcg tgatgccggg ttataaggtc atcgttgcag
     6541 gctgactata gccggaattg tccattgtct gcctgcagtt acaggaaaat tccgccttcg
     6601 ggcctatggc gctacgccgc accggcggct aatgtgaaat cgggcgcgac gaggggattt
     6661 tggttattgt tggtcaccaa ccaaacgcga cggtatttct gtcgccaaaa aagaccccac
     6721 ggaggaacac acgatgagac gattttcggc actgcttgca accgcggctg caacgatcct
     6781 gataacggca tcggcgcaag cgcaggtgat tttgaaggcc tcgcaccaat tcccgggcgg
     6841 gaagggcgat ccgcgcgacg aaatggtgca gatcatcgcc aaggaagcca aggctgccaa
     6901 tgttggcttg gaggtccagg tttttccggg tgcgtcgctg ttcaagccga acgatcaatg
     6961 gaacgccatg gtcaacggcc aactcgatat ctcttccttc ccgctcgact acgccagcgg
     7021 caaggtgcgc gccttcggtg ccacgctgat gccgggactc gtgcgcagcc acgatcgcgc
     7081 cgcgcgtatg aaaaattcgg agttcatgaa ggacatcaag gcaaaaattg aaaaggcagg
     7141 tgtggtcgtc ctggctgacg cgtggctcgc cggcgcggtc gcgtccaaaa aggcctgcat
     7201 ccgcaagccg gacgacatga agggactgaa aatccgttcc gccggcccga ccttcgcagc
     7261 gatgtggcag gaggcgggcg cttcgatcgt ctcgatcccg tcgaacgaag tctataatgc
     7321 gctgcagacc ggtgtcgccg acgccaccga cacctccacc ggaagcttcg tgtcgttccg
     7381 catctatgag caggtcaagt gcattaccgc gccgggtgaa aacgcgctgt ggttcatgta
     7441 cgagccggtg ctgatgtcga agaagagttt cgacaagctc gacaagaagc agcaggacgc
     7501 gctgatcgcg gccggcaaga agtcggagga gtatttcgcc aaggaaagca aggcgctcga
     7561 cgacaagatg gtcaaagcct tcaaggacca caatgtcgag gtcgtgagca tgacaccggc
     7621 cgagtaccag gcatggctcg acgttgccaa gaaaagttca tacgcccatt tcgccaagga
     7681 agttccggac ggcaagaagc tgatcgacga ggccttggct gtgaagtgat ttgcgatgcg
     7741 cagacgcggc cggcggcgac acgaccgccg gccgcaatgt gtctgggcat aagacacttt
     7801 aaggggcacc cggtccgatg gacttcttca tcctgacggt aaagcgtatt tcccagatct
     7861 gcggggtcat cgccgccggc ctgatcgcgg ccggtgtgct ggtggtctgc gagatggtgt
     7921 tcgtgcgcta cgttctcaac cagaacacga tctgggaaac cgacttcacc acctactgcc
     7981 tggttggcgc aaccttcatc ggcagtccct atgttctgct cacgcgtgga cacgtcaatg
     8041 tggacgtgtt gccgcattat ctcggccagg aggggcgcta ttggctggcc ctggcttcaa
     8101 tactcatgtc gctcgccttc tgtctcatca tgacggtgct tactttcaat ttctggcatg
     8161 aggcctggac ccaaaactgg gtgtccgaca cgatgtggcg cgcgcgcttg tggattccct
     8221 acagtaccat gccgatcggc cttggcctga tcacgctgca atatgttgtc gatctgattg
     8281 ccctgttgac cggtcgcgaa cccccatttg gcctctcgcc acgcgaaacc gccgaagatg
     8341 cggctcgctc gcaggcccgg caggcactgg gagacgttcc gtgagtccga ccactcaggg
     8401 ggcgatcgtc cttgtcgtga cgttgatcgt gctgctgtcc ggtgcgccgg tcgcgttcgg
     8461 gctcggtgcg atcgcgatcg cgttcctggt catcttccag ggctttgact cgctgcacgt
     8521 ggcggccgaa accttttaca ctgcattgca ggatttcacg ctcgtctcga tcccgatgtt
     8581 cgtaatgatg ggcgcggcca tcggctcttc gcccgcgggc aaggacctct atgaggcgct
     8641 cgaccgctgg ctttaccgcg tgccgggcgg gctcgtgatc tccaatctcg gtgcctgcgc
     8701 gatcttcgcg gcgctcaccg gttcatcgcc agcctgctgt gcggccatcg gcaagatggg
     8761 catcccggag atgcgcagcc gtggatatcc ggacgacgta gccaccggtt cgatatgcgc
     8821 cggcggtacg ctcggcatcc tgattccacc gtcgattacc ttcatcctct acgggatcgc
     8881 cacggagacg tcgatcggac ggctgttcct cgccggcgtg gtgccgggac tgatgctcac
     8941 cggcctgttc atactgtgga cgat
//
LOCUS       DASSRL010000034         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_508456, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000034 DASSRL010000000
VERSION     DASSRL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508456"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFL49_00700"
     CDS             <1..861
                     /locus_tag="VFL49_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET7910526.1"
                     /translation="SAVDIIRIAEGRVIVTGMGKSGHVARKIASTLASTGTPAFFVHP
                     SEASHGDLGMIANNDVILALSWSGETAEMKNITDYSRRHGIKLIAMTAIADSALAKVA
                     DVVLVLPQAREACPHNLAPTTSSLMQLALGDALAIALLESHGFTAVDFGLLHPGGKLG
                     TLLKTVRDIMHIGAAMPLAPLGTRMSDGILEMTTKGFGCVGIVDGRGLLVGIITDGDL
                     RRHMRNDLLDARVDDVMTRSPKTVRPDQLLSETLDILNSMKVTALFAVEAGKPVGMIH
                     VHDLLRTGAA"
     gene            complement(871..1818)
                     /gene="waaC"
                     /locus_tag="VFL49_00705"
     CDS             complement(871..1818)
                     /gene="waaC"
                     /locus_tag="VFL49_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HET7910527.1"
                     /translation="MPDILFIKTSSLGDVIHHMPAMIDARKAHPQSGFVWLVEEAFAP
                     LVRLHPAVHDVIPVAWRRWRKSLYAPATLSEITASLRAIRGRQFDDIVDTQGLLRSAL
                     IARAAQGRRHGYDSSSIREPLASVFYNERYNVSRDLHAVERNRTLSGLALGYSPQGAP
                     DFGLERTRFSCVGARYGLLLHSTARAEKLWPEEHWIALARRLGRDIELRLPWGTDAER
                     ARSERIAGAVPGARVEERRPLDQVATLIAGAQFVIGVDTGLLHLAAALGVPLVAIFAG
                     SRPGLTGPVGSGRIAVLGSNGAPPPVDAVLGAVEEVVVR"
     gene            1947..>2078
                     /locus_tag="VFL49_00710"
     CDS             1947..>2078
                     /locus_tag="VFL49_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7910528.1"
                     /translation="MTILVTGGAGYIGSHMVHELVDAGERVVVLDNLSTGFRFLIPAS
                     "
BASE COUNT          388 a          672 c          660 g          358 t
ORIGIN      
        1 tctgccgtcg atatcatccg gatcgcggaa ggccgggtga tcgtcacggg aatgggcaag
       61 tccggtcacg tcgctcgcaa gatcgcatcg actttggcat cgaccggcac gcctgctttt
      121 ttcgtgcatc cgagcgaggc aagccacggc gaccttggca tgatcgccaa caacgacgtg
      181 atcctcgcgc tgtcgtggtc gggcgagacg gcggagatga agaacataac cgactattcc
      241 cggcgccacg gcatcaagct catcgcgatg accgcgatcg ccgatagcgc gcttgccaaa
      301 gtggccgacg tggtcctcgt gttgccgcag gcgcgcgaag cctgcccgca caatctcgcg
      361 ccgacgacat catcgctcat gcagcttgct cttggcgatg cgcttgcgat tgccctgctg
      421 gaaagtcacg gctttaccgc ggtcgatttc ggccttctgc accccggcgg aaagctcggc
      481 acgctgctga aaaccgtgcg cgatatcatg catatcggcg cagcgatgcc gctggcgccg
      541 cttggtaccc gcatgtccga cggcattctc gagatgacca ccaagggatt cggctgtgtc
      601 ggcatcgtgg acgggcgcgg acttctggtc ggcatcatca ccgacggcga cttgcggcgg
      661 catatgcgca acgatctcct cgatgcgcgc gtcgacgacg tcatgacacg cagccccaag
      721 acggtacggc ccgaccagct gctgagcgag acgctggata tcctcaattc catgaaggtg
      781 accgcgctgt tcgctgtcga ggccggaaag ccggtcggca tgatccacgt gcacgacctc
      841 cttcgcaccg gcgcggcgta gaaatcatcg tcagcggacg acgacttcct caacggcgcc
      901 gagcacggca tcgaccggcg gcggcgctcc gttactgccg agtacggcga tgcggccact
      961 gccgaccggc cccgtgaggc cgggccggct gccggcgaag atcgcgacca gcggaacacc
     1021 cagagccgcg gcaaggtgca acagccccgt atccacgccg atcacaaatt gcgcgcccgc
     1081 tatcaatgtt gctacctggt cgagcggcct gcgctcctcg acgcgcgcgc cggggaccgc
     1141 acccgcgatg cgttcgctgc gcgcccgttc cgcatcggtg ccccacggca gccgcaactc
     1201 gatgtcccgg ccgagtcttc gcgcgagcgc gatccaatgc tcttcgggcc aaagtttctc
     1261 cgcccgcgcg gtgctgtgaa gcaacaggcc atagcgcgcg ccgacgcagg aaaaccgcgt
     1321 ccgctcgagg ccaaaatccg gcgcgccttg cggcgaataa cccagcgcaa gcccgctcag
     1381 agtgcggttg cgctcgaccg cgtgcaaatc gcggctgacg ttgtaccgct cattgtaaaa
     1441 taccgaagcc aaaggctcgc ggatgctgga ggagtcgtag ccgtgacggc gtccttgcgc
     1501 cgcgcgggcg atcagcgcgg aacgcagaag cccctgcgtg tccacgatat cgtcgaactg
     1561 acggccccgg atggcgcgca ggctggcggt aatttcgctc aaagtcgcag gcgcatagag
     1621 ggattttcgc cagcgccgcc acgcgacggg gatgacgtcg tggaccgcgg gatggagtcg
     1681 cacgagcggc gcgaaagctt cctcgacgag ccagacaaag ccggattgcg gatgcgcctt
     1741 tcgggcgtca atcatggccg gcatgtgatg aatcacatcg ccgagcgaag atgtcttgat
     1801 gaaaaggatg tcgggcatgg cgcgtttgtc gcacccatat accggtcatt aaggatggcc
     1861 gccataacca tgccatcgga cagcgcgatc aagacgcagt ttttactgtc ggccggcaca
     1921 ttggcgcacg tgcgagggac aatggaatga cgatcctggt gactgggggt gccggctata
     1981 tcggcagcca catggtgcac gagctcgtgg atgccggcga gcgcgttgtc gtgctggaca
     2041 acctgtcgac cggctttcgt tttctcatcc cggcatcc
//
LOCUS       DASSRL010000035         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_875850, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000035 DASSRL010000000
VERSION     DASSRL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875850"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VFL49_00715"
     CDS             <1..1711
                     /locus_tag="VFL49_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HET7910529.1"
                     /translation="IPEIFPEVFRGHGGAPAVYFEAAAVITVLVLMGQVLELRAREAT
                     SGAIRALLDLAPKTARLVKDDGNDEEVSLDSVHPGDRLRVRPGDKVPVDGVVLEGRSA
                     LDESMVTGESMPVTKEKDSRVIGGTLNKSGSFVMRAEKVGRDTLLSQIVQMVAGAQRS
                     RAPIQRLADQVSAWFVPAVIAVAIIAFAAWATFGPEPRFAYGLVAAVSVLIIACPCAL
                     GLATPMSIMVGVGRGAQAGVLIKNAEALERMEKIDTLVIDKTGTLTEGRPKVVAISPM
                     AGFDETQVLQLAASVERGSEHPLAAAIVAAAKERNIELMNVTGFDSPVGKGVVGTVNG
                     KKLALGNARFLNELGINTSALAPEAERLRADGATAIMLAVNGKPAGAIAIADPVKATT
                     AEALKALADDKIRVVMLTGDNRTTAHAVARRLGIGEIEAEVLPDQKSAVVEKLRREGR
                     VVAMAGDGVNDAPALAAAEVGIAMGTGADVAIESAGVTLLKGDLTGIVKARALSEAVM
                     RNIRQNLFFAFIYNALGVPVAAGVLYPVFGILLSPVIAAAAMALSSVSVVGNALRLRR
                     VRL"
     gene            complement(1715..1876)
                     /locus_tag="VFL49_00720"
     CDS             complement(1715..1876)
                     /locus_tag="VFL49_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HET7910530.1"
                     /translation="MGILISILITFLVIILVLYLVNMLPLDGRAKQIVRVIVIIIGVL
                     SLLKYLAVF"
     gene            complement(1970..2332)
                     /locus_tag="VFL49_00725"
     CDS             complement(1970..2332)
                     /locus_tag="VFL49_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HET7910531.1"
                     /translation="MTVRASLLISALAAAVLSGCAASEDTAGRFLVQPDKYVLYSCKE
                     LAEAAQAISARQLELERLIAKARTGSGGELVSDLAYRPEYVQLRGQMNEVHRTSAEKN
                     CKFSPAAPGPHASDQVIR"
     gene            complement(2429..2812)
                     /locus_tag="VFL49_00730"
     CDS             complement(2429..2812)
                     /locus_tag="VFL49_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7910532.1"
                     /translation="MRRVLYVEDNDDNVYMLKLRFDALDGFEFLTAADGEAGYAKARA
                     ELPDVILMDLDLPVIDGWEVTRRLKQDVATRDIPIIALTAHAMAGSREKAIAAGCDEF
                     DTKPINFDRLMQKIDRLIASSCSRE"
     gene            complement(2809..>3396)
                     /locus_tag="VFL49_00735"
     CDS             complement(2809..>3396)
                     /locus_tag="VFL49_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7910533.1"
                     /translation="GWTVLSALRGNPQLADIPVVMATITDEQRKGMALGAAGYLTKPI
                     DRDQLIALLTPFQARARRTHVLIVEDDFAQRILMRSVLEPQQWVVTEADNGRVALDRL
                     AEETPDIVLIDLMMPEMDGFELIKALRGRPDWRCIPVIVITALDLTSADRQRLNSGIE
                     AILSKNSLDPAQLVETVRQVVANARRRNLISEVAS"
BASE COUNT          647 a         1067 c         1041 g          641 t
ORIGIN      
        1 catccccgaa attttcccgg aggtgttccg cggccatggt ggcgcgccgg ccgtctattt
       61 tgaggcagct gccgtcatca ccgtgctggt cctgatgggc caggtcctcg agctgcgcgc
      121 gcgcgaggcg acctcgggcg caatccgcgc cctgcttgat cttgcgccga agactgcccg
      181 cctcgtgaaa gacgacggca acgacgaaga agtctcgctc gatagcgtgc atcccggcga
      241 tcgcttgcgc gtgcgcccgg gcgacaaagt gccggtcgac ggagtcgtgc tggagggccg
      301 ttccgcgctt gatgaatcga tggtgaccgg cgagtcgatg ccggtgacca aggaaaagga
      361 ttcgcgggtc attggcggta ccctcaacaa gtccggcagc ttcgtgatgc gcgccgagaa
      421 agtcggccgc gataccttgt tgtcgcagat cgtgcagatg gtcgcaggcg cgcagcgcag
      481 ccgggcgccg atccagcgcc ttgccgatca ggtctctgcc tggtttgtac cggcggtgat
      541 cgccgtggcg atcattgctt tcgcggcctg ggcaacattc ggccctgagc cgcgcttcgc
      601 ctatggcctg gtcgccgccg tcagcgtcct gatcatcgcc tgcccttgcg cgctcggcct
      661 ggcaacaccg atgtcgatta tggtcggcgt cggccgcggc gcccaggccg gcgtcctgat
      721 caagaacgcc gaggcgctgg agcgcatgga aaagatcgac acgctcgtca tcgacaagac
      781 cggcaccctc actgaaggca ggccgaaggt cgtcgccatt tcgccgatgg ccggcttcga
      841 tgaaacgcaa gtccttcagc ttgccgcaag tgtcgagcgc gggagcgagc accctctcgc
      901 ggccgcgatc gttgccgcgg cgaaggaacg caacatcgag ctgatgaatg tcaccggctt
      961 cgattcgccg gtcggaaagg gcgtcgtcgg cacggtcaac ggcaagaagc tcgcactcgg
     1021 caatgccaga tttctcaacg aactcggcat caatacatcc gccctcgccc ccgaagccga
     1081 gcgcctgcgc gcggacggag cgacggccat tatgctcgcc gtcaacggca agcctgcggg
     1141 cgccatcgcg attgccgatc cggtgaaggc cacaacggcc gaagccctga aggcgctcgc
     1201 cgatgacaaa atccgcgtcg tcatgctcac cggtgacaat cgcaccacgg cgcacgcggt
     1261 tgcgcgccgg ctcggcattg gcgaaatcga ggccgaagtt ctgccggatc agaaaagcgc
     1321 ggtggtcgag aaactgaggc gcgaaggccg ggtcgtcgcc atggccggtg acggcgtgaa
     1381 tgacgcccct gccctggcag cagccgaagt cggcattgcc atggggacag gcgcagacgt
     1441 cgccatagaa agtgcgggcg tcaccctgct gaagggcgac ctgaccggaa tcgtcaaggc
     1501 gcgcgcattg tcggaagctg tgatgcgcaa catccggcag aacctgttct tcgctttcat
     1561 ctacaatgcg ctcggcgtgc cggtcgcggc cggcgtgctc tatcccgtct tcgggatact
     1621 gctgtcgccg gtcattgcag ccgctgcaat ggcactttca tcggtcagcg tcgtcggaaa
     1681 cgccctgcgc cttcgtcgcg tgaggctgtg agcgctagaa gacagcgaga tatttcagca
     1741 gcgacagcac gccgatgatg atgacgatga cgcgaacaat ctgtttggcg cgcccgtcga
     1801 gcggcaacat gttgacgagg tagaggacga ggataatgac aagaaacgta ataagaatgc
     1861 tgatcagaat tcccatgcta gcccccttgt gatgccacga acatggcagg ttccaagcct
     1921 tacaagttcc acagtctatg aaagttccat cacaggcggt acgccgaatt cagcggatga
     1981 cctgatcact ggcatgcggg ccgggagcgg cggggctgaa tttgcagttc ttttcggcag
     2041 acgtcctgtg cacctcgttc atctgaccgc gcaattgcac atattcgggc cggtacgcga
     2101 gatcgctgac gagttcgccg cccgagcccg tccgcgcctt ggctatcagc ctctcgagct
     2161 ccagctgccg cgcactgatc gcctgtgccg cctcggcaag ctctttgcag ctatagagaa
     2221 cgtatttgtc gggctggacc aggaagcgcc cggcggtgtc ttccgacgcc gcgcagccgg
     2281 acaacacggc agctgcaagc gcgcttatca gcagtgaggc ccggacggtc atcgaaagtt
     2341 gctatcgcac gattctggca ttgcggtgga gccgatcatt ctccggcgtc ggcacccagc
     2401 cagggccgcc gtctcgggcg cggcgcgatc attcacgcga gcagctgctc gcaatcaggc
     2461 gatcgatctt ctgcatcagg cggtcgaaat tgatgggttt ggtatcgaat tcgtcgcagc
     2521 ctgccgcaat cgccttttcg cgggagcctg ccattgcatg agccgtcagc gcgatgattg
     2581 gaatgtcccg ggtcgccaca tcctgtttga gccggcgcgt cacttcccag ccatcaatca
     2641 ccggcaagtc cagatccatc aggataacat cgggcaattc ggccctggct ttcgcatacc
     2701 cggcttcgcc atcagcggct gtcagaaatt caaatccgtc gagcgcatca aatcgcaatt
     2761 tgagcatata gacattgtcg tcgttatcct cgacgtaaag aactctcctc acgaggccac
     2821 ctccgatatc aggttcctgc ggcgcgcatt ggcgacaacc tggcgaacgg tttccacaag
     2881 ctgggccgga tcgagcgagt tcttcgacag gatcgcttcg attccggaat tgagtcgttg
     2941 gcggtccgcc gacgtgaggt caagcgccgt tatcacgatc accgggatgc agcgccagtc
     3001 cggccgcccg cgcaacgcct tgatcagctc gaacccgtcc atttccggca tcatcaggtc
     3061 gatcagaacg atgtccggtg tttcttccgc cagccgatcg agcgccacgc ggccattgtc
     3121 ggcttcggtc acaacccatt gctgaggctc cagaacggat cgcatcaata tccgctgggc
     3181 gaaatcgtct tcgacaatga gcacatgggt tcgccgcgcg cgggcctgaa atggcgtcag
     3241 cagcgcaatc aattgatccc ggtcaatcgg cttggtcagg tagccggccg cgccaagcgc
     3301 catgcccttt cgctgttcgt cagtaatcgt cgccatgacc accggaatat cggcaagctg
     3361 cggatttccg cgaagcgccg agagaacggt ccatcc
//
LOCUS       DASSRL010000036         6355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1418314, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000036 DASSRL010000000
VERSION     DASSRL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418314"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFL49_00740"
     CDS             complement(<1..342)
                     /locus_tag="VFL49_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7910534.1"
                     /translation="MTALHYDVLGIGNAIVDVIARAEDDFLVAQGMNKGTMALIDEAR
                     AQAIYAAMGPAVESSGGSAANTIVGVASFGARAAFVGKVKDDELGRSFIHDIRAAGVA
                     FDTKPETTGPST"
     gene            466..2010
                     /gene="murJ"
                     /locus_tag="VFL49_00745"
     CDS             466..2010
                     /gene="murJ"
                     /locus_tag="VFL49_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7910535.1"
                     /translation="MSLARDITTVGGGTLMSRLLAYARDAWIAALLGAGPYSETLFAV
                     LQVVNFFRRLLSEGALNSAFVPIWSKLHGSTNGAANANRFTRRVLLMMFCVAGSIVLL
                     AVFLGRYIIAGIAPGFDDAQQFFAALLLSITAPYILLAGLVAVIAAALSAEGRVGAVA
                     ITTVLFNLTMLLALLFVLSVSLERYETTAWVALAVTAAGTVQLLTIVTVWLMTGKRWQ
                     RTNAAVRDQVGLFFGRALPGLIATGIPQLKLIAAIAIVSASPSAVSWLYYANRLYELP
                     LGIASIGVAAAIVPRIASSVHARDTGRFAAVQSRAYELSVGLALPAATGFALLAEPIA
                     RGLFERGAFGAQDTAAVAAALVAICAGLPGHVLEKVFGAVSFAHEDTHTPMLAALSGL
                     AAAIAGGVLLLSRFDCVGVAAAIAMSGWVGAGLLGATLWRRGWLRIDTDARRRLPRIC
                     LATLAMAGVVGYSNHAIGTAGASSAARLAILAALVTLGIAVYLAALRLLRVMTLKELA
                     ALQRKS"
     gene            2071..3105
                     /gene="trpS"
                     /locus_tag="VFL49_00750"
     CDS             2071..3105
                     /gene="trpS"
                     /locus_tag="VFL49_00750"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7910536.1"
                     /translation="MAFRERVFSGVQPTGNLHLGNYLGAITKFVALQERYDCIYCVVD
                     LHAITVWQDPSELPRATREVTAAFIACGIDPKKHIIFNQSQVAEHAELAWVFNCVARV
                     GWLNRMTQFKEKAGKDRENASVGLYVYPSLMAADILVYRATHVPVGEDQKQHLELSRD
                     IAQKFNNDFSASIQAHGYGDVFFPQPEPIIQGPATRVMSLRDGTKKMSKSEASDYSRI
                     NLTDDADAIAQKIRKAKTDPEPLPGEEKGLVPRPEADNLVGIYAALQGVSKAEVLGQF
                     GGGQFSTFKTALADLAVAKLGPIGAEMKKLLQDPVYIDSILADGSQRAQAIAAETMKA
                     VKDIVGFVRR"
     gene            3213..3707
                     /locus_tag="VFL49_00755"
     CDS             3213..3707
                     /locus_tag="VFL49_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7910537.1"
                     /translation="MSSKRRSYEAGHKRKYLVVIDDSEECDRAVYWAAKRAGRTKSQI
                     VMLRVIEPGDRNQQWLGVADIMKAEAQEAANAALDKFAARAKKIAKITPDRVIREGNT
                     SEEIIKLIDEDADIGILVLAAGIGKEGPGPLVSNLAKTAGTFPIPVALVPGHLSDDDI
                     DASS"
     gene            3816..4376
                     /locus_tag="VFL49_00760"
     CDS             3816..4376
                     /locus_tag="VFL49_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET7910538.1"
                     /translation="MFIQTETTPNPATLKFLPGRPVLESGTLDMPTKEAAAQSPLAAK
                     LFDVPNVGAVFFGSDFIAVTKTGGEWQQLKPAILGAIMEHYMSGAPLVSGDAAQPDAP
                     ADEFFDAKDADTVATIKDLIETRVRPAVASDGGDITFRGYKEGIVYLNMKGACSGCPS
                     STATLKHGIQNLLRHFVPDVVEVRPI"
     gene            4534..5217
                     /gene="tsaB"
                     /locus_tag="VFL49_00765"
     CDS             4534..5217
                     /gene="tsaB"
                     /locus_tag="VFL49_00765"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7910539.1"
                     /translation="MRVLAIDTALEACSVAVLDTERADLRAHESLPMQRGHAEALMPL
                     IARVLDRVQLDFSAIDRVAVTTGPGSFTGLRVGIAAARGIALAAGKPAIGLTTLAAFA
                     APFIAADDTLPVVAAIDARHDHVYLQVFGPGGRTLVAPRLASLRDALRVSATGAPRVA
                     GTAAAKLATQWPAGERAPTKVEQRSAPDINWVARLGAAATDTGTPPRPLYLRPPDAHP
                     QDAVQLARR"
     gene            5214..5699
                     /locus_tag="VFL49_00770"
     CDS             5214..5699
                     /locus_tag="VFL49_00770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7910540.1"
                     /translation="MIGFFSSLFGRAEPAIMEASQRDAPSIAALHGSSFRRGWSEQEV
                     EGLLLDRHVIAHRATARTHLTGFIMSRLVEDEAEILSVAVADRQRGRGLARQLLNFHL
                     RRLAGLGARAIFLEVDEHNIPALRLYQRAGFQEVSRRPGYYPGTNMTAATALILRRDL
                     V"
     gene            5847..6230
                     /locus_tag="VFL49_00775"
     CDS             5847..6230
                     /locus_tag="VFL49_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7910541.1"
                     /translation="MRMTEQRRVIARVLAESDDHPDVEELYRRCAKVDEHISISTVYR
                     TMRLFEDAGIIERHDFRDGRARYETSPDAHHDHLINLRTGEVIEFQSEEIERLQAEVA
                     RKLGYKLVDHRLELYAVPLSDDKKR"
BASE COUNT         1134 a         2031 c         1989 g         1201 t
ORIGIN      
        1 cgtggatggg ccggtcgttt ccggcttggt gtcgaaggca acgccggcgg cacgaatgtc
       61 gtgaatgaaa ctgcgcccga gctcgtcgtc tttcaccttg ccgacgaatg ccgcgcgcgc
      121 cccgaaactg gcgacgccga cgatcgtgtt ggccgcagag cctcccgaac tttccaccgc
      181 cggacccatc gccgcgtaga ttgcctgcgc gcgggcttca tcgatcagcg ccatcgtgcc
      241 tttgttcatg ccctgtgcca ccagaaaatc gtcttcagcg cgggcgatca catcgacgat
      301 ggcattgccg ataccgagca cgtcatagtg aagagcagtc atgtgtcatc cttttgaatg
      361 gcgcgcgcac tatatcggcg catcgagcga cggcaagctt atgcgcattg catgatggcg
      421 ggcgcgtgcc cacgggccta aagtagcggt cagggaatca ccaaaatgtc gctcgcacgc
      481 gatatcacca ccgtcggcgg cggcacattg atgtcgcgtc ttcttgccta tgcgcgcgac
      541 gcctggatcg cggccttgct cggtgccgga ccctactccg aaaccttgtt cgcggttctg
      601 caggtggtca atttttttcg ccgcctgttg tcggagggcg cgctcaacag cgccttcgtt
      661 ccgatctgga gcaagctgca cgggagcacg aacggcgcag ccaatgcaaa ccgttttacg
      721 cggcgcgtgc tgctcatgat gttctgcgtg gcaggcagca tcgtcctgct cgcggttttt
      781 ctcggccgtt atatcatcgc cggcatcgca ccgggctttg acgacgcgca gcaatttttt
      841 gcggcacttc tgctgtccat aaccgcacca tatattctgc ttgcgggact tgtcgcagtc
      901 atagcggccg ctctgagcgc cgaaggccgc gtcggcgccg tcgccatcac gacggtcttg
      961 tttaatctga cgatgctgct ggcgctgctg tttgtgctca gcgtgtcact cgagcgctat
     1021 gaaaccaccg catgggtcgc gcttgccgtc accgcagcgg gcacggtcca gcttctgacc
     1081 atcgtcaccg tctggctgat gaccgggaag cgctggcaac gaacgaacgc ggcagtgcgc
     1141 gatcaggtcg gactgttctt cggacgggca ctccctggct tgatcgcgac cggcattccg
     1201 cagctgaagc tgatcgcagc gatcgccatc gtctcggcat cgccttccgc agtctcatgg
     1261 ctctattatg ccaatcggct ctacgaactg ccgctcggca tcgcctctat cggcgtcgcg
     1321 gccgcgatcg tgccgcgcat cgcgagcagc gtgcatgccc gcgacacagg ccggttcgcc
     1381 gcagtgcaat cacgcgccta tgagctctcc gttggcctcg ctttgccggc ggccacgggt
     1441 ttcgcgctgc tggcagagcc gattgcacgc gggctgttcg aacgcggcgc cttcggcgcg
     1501 caggacacgg cggcggttgc cgctgcactg gtcgcgatct gcgccggact tccggggcat
     1561 gtgctggaaa aagtgttcgg cgcggtctct ttcgcacacg aggacacgca tacgccgatg
     1621 cttgcggcgc tttctggcct cgccgcggcc attgccggcg gcgtgctgct gttgtcgcgc
     1681 ttcgattgcg tcggcgtcgc ggcggcgatc gccatgtcgg gctgggtcgg cgccggcctg
     1741 cttggcgcca cgctttggcg gcgcggttgg ctgcggatcg atactgatgc ccgacgccgc
     1801 ctgccgcgta tctgccttgc cacgctcgcg atggccggcg tggtcggcta ttccaatcat
     1861 gcgatcggca cggctggtgc gtcatcggcc gcacggctgg cgatcctcgc cgcgctggtc
     1921 acgctcggca tcgccgtcta cctcgcggcg ttacgactgc tccgcgtcat gacacttaaa
     1981 gagctggccg ccctgcagcg aaaatcgtaa aggccttgcg cggcgcgcct cgggcgtggc
     2041 atggctcccg cgcaaaggaa gagggtggac atggcgttca gggagcgcgt tttttccggc
     2101 gtgcagccga ccggcaacct gcatctcggc aattacctcg gcgcgatcac caagttcgtg
     2161 gctttgcagg aacgctatga ctgcatctac tgcgtcgtcg atcttcacgc catcaccgtt
     2221 tggcaggatc cgagtgaatt gccgcgcgcc acgcgcgagg taaccgcggc gttcattgcc
     2281 tgcggcattg atccgaagaa gcacatcatc ttcaaccaga gccaggtcgc cgagcacgcc
     2341 gaactcgcct gggtcttcaa ttgcgtcgcg cgcgtcgggt ggctcaaccg gatgacccag
     2401 ttcaaggaga aggccggcaa ggaccgcgag aacgcgtcag tcggtctcta cgtgtatccg
     2461 agcctgatgg cggccgatat cctcgtctac cgcgcaacgc acgttcccgt cggcgaggac
     2521 cagaagcagc acctggaact gtcgcgcgac atcgcgcaga aattcaacaa cgacttctcg
     2581 gcgtcgatcc aggcgcacgg ctacggcgac gttttctttc cgcaaccgga gccgatcatc
     2641 cagggtccgg cgacccgcgt gatgtcgctg cgcgacggta cgaagaaaat gtcgaagtcg
     2701 gaggcatctg attactcccg catcaatctg accgacgatg cggacgccat cgcgcagaag
     2761 attcgcaagg ccaagaccga tccggaaccg ttgccgggcg aggagaaagg tctcgtaccg
     2821 cgtccggaag ccgacaatct ggtgggcatc tacgcggcgc tgcaaggcgt gagcaaagcc
     2881 gaagtgcttg gccaattcgg cggcggccag ttttcgacct tcaagaccgc gcttgccgat
     2941 ctcgccgttg cgaaactcgg cccgatcggt gccgagatga agaaactgct gcaggatccg
     3001 gtctacatcg actcgatcct ggccgatggc tcgcagcgcg cgcaagcgat cgccgccgaa
     3061 accatgaaag ccgtcaagga tatcgtcggc ttcgtacgcc gctagtttgc agcgcggaac
     3121 acccgccttg tcgtcgcgcc gggcggcgtg ccaccattgc ccgcttgtgc attcatcaca
     3181 gcgcatatga atcggacggg agcgtcgcga cgatgagcag taagcgccgc agctatgagg
     3241 ccggccacaa gcgaaaatat ctggtggtca tcgatgacag cgaggaatgc gaccgcgctg
     3301 tttactgggc ggcgaagcgc gcgggccgca cgaagtcgca gatcgttatg ctgcgcgtaa
     3361 tcgagcctgg cgatcgcaac cagcaatggc tcggcgttgc agacatcatg aaagcagagg
     3421 cgcaggaagc ggcaaacgcc gcgctcgaca aattcgccgc gcgcgcaaag aagatcgcca
     3481 agatcacgcc cgaccgcgtc attcgcgagg gcaacacgtc ggaagaaatc atcaaactga
     3541 tcgacgagga cgccgacatc ggcatactgg tgctggctgc aggcatcggc aaggaaggtc
     3601 cgggaccgct ggtgtcgaac ctcgccaaaa ccgccggcac gttccccatc ccggtcgcgc
     3661 tcgtgccggg tcatctgagc gacgacgaca tcgacgcaag ctcgtgattt gcggccattt
     3721 ccgactggtt gaccggaagc tccgcaagcg ccatttatgg ttcatggttt gaccgcccgc
     3781 gggccttgaa cccgttgagg caaggagcga tttcgatgtt catccagacc gagaccaccc
     3841 ccaatcccgc gacgctgaaa ttcctgcccg gccgccccgt gctcgagagc ggcacgctcg
     3901 acatgccgac gaaggaagca gcggcgcaat cgccgctcgc tgccaaactg ttcgacgtgc
     3961 ccaatgtcgg ggcggtattc ttcggatccg atttcatcgc ggtgaccaag accggcggcg
     4021 aatggcagca actcaagccg gcgatcctcg gcgccatcat ggagcactac atgtcgggtg
     4081 cgccgctggt ttccggcgac gcggcccagc ccgatgcccc ggcggatgaa ttcttcgatg
     4141 ccaaggatgc cgacactgtc gccaccatca aggatctgat cgagacgcgc gtgcgtcctg
     4201 ccgtggcgag tgacggcggc gacattactt tccgcggtta caaggaaggg atcgtctatc
     4261 tcaacatgaa gggcgcctgc tcaggttgcc cgtcatcgac ggcgaccctc aagcacggca
     4321 tccagaatct gctcaggcat ttcgttcccg atgtcgtcga agtgcggcca atatagccca
     4381 tctgagcaat ttccaaagtc atgcccggcc atacccgtcc gaaggacggc gtgcttcgca
     4441 cgcctgtccg cgaggtatcg acggtttagc gttgttgtga agacgctgat agcgcgctga
     4501 caagcctggc catgacggtg gtaaactcaa gtcatgcgcg tcctcgccat cgataccgcg
     4561 cttgaggcct gctccgtcgc tgtgctcgac accgagcgcg cggatttgcg cgcgcatgaa
     4621 tcgctgccga tgcagcgcgg gcacgccgag gcgctgatgc cgctgattgc gcgggtgctg
     4681 gaccgcgtgc agctcgactt ctcggctatc gaccgcgtcg cggtcactac tggacccgga
     4741 agcttcacag ggttgcgtgt gggcatcgcc gccgcgcgcg gcattgcgct tgccgcaggc
     4801 aagccggcga tcgggctgac gacgcttgcg gcctttgccg cgccgttcat tgcggccgac
     4861 gatacgcttc ccgtggtggc cgcgatcgac gcgcggcacg accacgtata tctccaggtg
     4921 ttcgggcccg gaggtcgcac gctcgttgcg cctcgccttg catcgctgcg cgatgccttg
     4981 cgcgtttcgg caaccggtgc gccacgcgtc gctggaaccg ctgccgccaa actcgccacg
     5041 cagtggcctg caggcgagcg ggcgccgacg aaagtcgagc agcgcagcgc acctgacatc
     5101 aattgggttg cgcgtctcgg cgccgctgcg accgacaccg gcacaccgcc cagacctttg
     5161 tatctccgtc cgcccgacgc gcacccgcag gatgcggtgc aattggcacg tcgatgatag
     5221 gcttcttcag cagcctgttc ggccgcgccg agccggcgat aatggaggcg agccagcgcg
     5281 acgcaccttc gatcgcggcg cttcacggca gctcattccg gcgcggctgg agcgagcagg
     5341 aggttgaagg cctgctgctc gatcggcatg tgatcgcgca tcgggcgaca gcccgcacgc
     5401 atcttacggg cttcatcatg tcacggctcg tcgaagacga agcggaaatt ttgtccgttg
     5461 ccgtcgccga ccggcagcgc ggccgcgggc tcgcgcggca gcttttgaac tttcatctgc
     5521 ggcgccttgc cggcctcggt gcgcgcgcca tttttctgga agtcgatgag cacaacattc
     5581 cggcgttgcg gctttatcag cgtgccgggt ttcaggaagt ctcccgccgc cccggttatt
     5641 atccggggac caacatgacc gctgccaccg cgctgattct gcggcgcgac ctggtctgaa
     5701 cgcgcggtgg acacacgcct gacgccgcaa atatgatatc aggggcggca gcgaaaggtc
     5761 tgccccagag ggatgccgac gtgagcgccg tgagcaagca cactatcgaa aagaagaaca
     5821 tcgaggcgct atgtgcagct aaaggcatgc gcatgaccga gcagcgacgc gtaatcgcgc
     5881 gcgtgctggc tgaatcggac gaccatcccg acgttgagga gctgtaccgg cgctgtgcga
     5941 aggtggacga acatatttcg atttcgaccg tctaccggac catgcggctt ttcgaggatg
     6001 ccggcatcat cgagcggcac gactttcgcg acggacgggc gcgctatgaa acaagcccgg
     6061 acgctcatca cgatcatctg atcaacctgc gtaccggcga ggtgatcgaa ttccagtccg
     6121 aggaaatcga gcgtctgcag gccgaagtcg cgcgcaagct cggctacaag ctggtcgatc
     6181 atcggctcga gctttatgcc gtgccgctga gcgacgacaa gaagcgctag gcatgattcg
     6241 ccggaactga acgccgatgg cgcgcgcgct tttcattgcg gcattttttt tcacgatgac
     6301 ccccctgctg atctcgatgc agtggctgct gggcaagctc aggttgcccg gttgg
//
LOCUS       DASSRL010000037         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_71174, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000037 DASSRL010000000
VERSION     DASSRL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71174"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /gene="rpsH"
                     /locus_tag="VFL49_00780"
     CDS             <1..196
                     /gene="rpsH"
                     /locus_tag="VFL49_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7910542.1"
                     /translation="DGEPVIREIERVSKPGRRVYVAVRNLPRINNGLGVAILSTPKGV
                     MADHAAREANVGGEIICTVF"
     gene            196..729
                     /gene="rplF"
                     /locus_tag="VFL49_00785"
     CDS             196..729
                     /gene="rplF"
                     /locus_tag="VFL49_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7910543.1"
                     /translation="MSRIGKKAVVVPTGVTANVEGQTVKVKGPKGALQVVVHDDVSVA
                     MDKTGIKLEPRSETKRARSMWGTSRTLVANVMAGVTKGFEERLEITGVGYRAAVQGKS
                     LQLQLGFSHDVNFPIPEGIAITTPKPTEIVVTGVDKQKVGHVAAEIRGFRPPEPYKGK
                     GVKYVNEYIFRKEGKKK"
     gene            740..1102
                     /gene="rplR"
                     /locus_tag="VFL49_00790"
     CDS             740..1102
                     /gene="rplR"
                     /locus_tag="VFL49_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7910544.1"
                     /translation="MSRLTDRTARRTATVRRKIRLVARGRTRLSVFRSSKHIYAQLID
                     DNKGVTLTAASSLEKAMREGAKTGANIAAAKAVGKLIAERAKEKGITDVVFDRGGYLY
                     HGRIKALADAAREGGLNF"
     gene            1117..1695
                     /gene="rpsE"
                     /locus_tag="VFL49_00795"
     CDS             1117..1695
                     /gene="rpsE"
                     /locus_tag="VFL49_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7910545.1"
                     /translation="MAHEPRRERGERGGRDREERDSEFTDKLVHINRVAKVVKGGRRF
                     GFAALVVVGDQKGRVGFGHGKAREVPEAIRKATDAAKRGLTRVPLREGRTLHHDVLGR
                     HGAGKVFLRAAPPGTGIIAGGPMRAVFETLGMQDVVAKSLGSSNPYNVVRATFDALKN
                     QDSPRSVAARRNIKVSTLQSRRRDGDAEAAAD"
     gene            1707..1901
                     /gene="rpmD"
                     /locus_tag="VFL49_00800"
     CDS             1707..1901
                     /gene="rpmD"
                     /locus_tag="VFL49_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7910546.1"
                     /translation="MVQGKTIKVEQVGSPQRRHFDQRATLIGLGLNKIGRTRDLPDTP
                     STRGMIRKVQHLVKVVGEAK"
     gene            2022..>2228
                     /gene="rplO"
                     /locus_tag="VFL49_00805"
     CDS             2022..>2228
                     /gene="rplO"
                     /locus_tag="VFL49_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7910547.1"
                     /translation="MKLNQIADRPGARKKRMRIGRGIGSGMGKTGGRGGKGQTARAGV
                     RLGGFEGGQMPLHRRLPKRGFNSRD"
BASE COUNT          470 a          656 c          761 g          341 t
ORIGIN      
        1 cgacggcgag cctgtgatcc gcgagatcga acgagtctcg aagccgggac gccgcgtcta
       61 tgtcgcggtg cgtaatttgc cccgcatcaa taacggcctc ggcgttgcga ttctttcgac
      121 accgaaaggc gtgatggccg accatgctgc ccgcgaggcc aatgtgggcg gcgaaatcat
      181 ctgcacggtg ttctgatgtc gcgcatcggc aaaaaagcag tcgtggtccc gaccggcgtt
      241 accgccaacg tcgaaggtca gaccgtgaag gtgaaggggc caaagggcgc cctccaggtc
      301 gttgtgcacg acgacgtctc tgtcgccatg gataagaccg gaatcaagct cgagccgcgc
      361 agcgagacaa agcgcgcgcg ctcgatgtgg ggcacgtcgc gcaccctggt cgctaacgtg
      421 atggcgggcg tgaccaaagg tttcgaagag cggctggaga tcaccggtgt cggctatcgc
      481 gctgcggtac agggcaagag cctgcagcta cagctcgggt tctcgcacga tgtcaacttt
      541 ccgattccgg aaggcatcgc gatcacgacg ccgaagccga ccgaaatcgt ggttaccggt
      601 gtcgacaagc agaaggtcgg gcacgtagcc gccgaaatcc gcgggttccg gccgcctgag
      661 ccgtacaagg gcaagggcgt gaaatacgtc aacgagtaca tcttccgcaa ggaaggaaag
      721 aagaagtaga ggacgcgcca tgtcgagact tacagaccgg accgcccggc ggacggcgac
      781 ggtgcgacgc aagatcagac ttgtggcgcg cggacgcacg cggctgtccg tattccgctc
      841 gtcgaagcat atctatgcgc agctcatcga cgataataaa ggcgtcaccc ttacggcggc
      901 gtcatcgctg gaaaaagcga tgcgcgaggg cgccaagaca ggtgcaaaca tcgctgcggc
      961 aaaggcagtt ggcaagctga tcgccgagcg agccaaggaa aaaggcatca cggacgtcgt
     1021 gttcgatcgc ggcggctatc tctatcacgg acgcatcaag gcgctggcgg atgccgcgcg
     1081 tgaaggcgga ctcaactttt aacggaacga gcgcacatgg cacatgaacc ccgacgagag
     1141 cgcggcgaac gcggcgggcg cgaccgcgaa gagcgcgaca gcgagttcac cgacaagctg
     1201 gtacatatca accgcgtggc aaaggtggtg aaaggtggcc gccgtttcgg ttttgccgcg
     1261 ctggttgttg tcggcgatca gaaaggccgg gtcgggttcg gccatggcaa ggcgcgcgag
     1321 gtgccggaag cgatccgcaa ggcgacagat gcggcgaagc gtggcttgac ccgcgttccg
     1381 ctgcgcgagg gccggacgct gcaccacgat gtgctcggac ggcacggcgc cggcaaggtg
     1441 ttcctgcggg cagcccctcc gggcaccggc atcatcgccg gcggtccgat gcgcgcggtg
     1501 ttcgagacac tcggcatgca ggacgtcgtt gcgaaatcgc tgggctcgtc caatccgtac
     1561 aacgtcgtgc gggcgacatt tgatgcgctc aagaatcagg attcaccgcg ttcggtggca
     1621 gcccgccgca acatcaaggt ctcgacgttg caatcgcggc gccgcgatgg cgatgccgag
     1681 gcggcggctg actaagagga ctgggcatgg ttcagggaaa gacgatcaag gtagaacagg
     1741 tgggcagccc gcaacgacgc cacttcgatc agcgggcgac gctgatcggg ttgggtctca
     1801 acaagattgg gcgcacacgc gatttgccgg acacgccatc gacccgtggc atgatccgca
     1861 aagtgcagca tctggtgaaa gtagttggcg aggcgaaata acagtttcgt cgtcgccggg
     1921 cttgccccgg tgacttcgct tggcgaggca tagtgccgca ccaagcgaga tggccgggac
     1981 gaccccggcc atgacaaacg agaggtcaaa ggacgagaac gatgaaactc aaccagatcg
     2041 ccgacaggcc tggcgcgcgt aagaagcgca tgcgtatcgg ccgcggcatc ggctccggca
     2101 tgggtaagac cggcggccgc ggcggcaagg gccagaccgc gcgcgcgggc gtccggctcg
     2161 gcggcttcga gggtggccag atgccccttc accgccgcct gcccaagcgc ggcttcaact
     2221 cgcgcgac
//
LOCUS       DASSRL010000038         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_980873, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000038 DASSRL010000000
VERSION     DASSRL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980873"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFL49_00810"
     CDS             complement(<1..896)
                     /locus_tag="VFL49_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7910548.1"
                     /translation="MNIQNQPATAANGKGRRRPPRTPKGRQVDPQALEEIRALLTDRP
                     RRRDLLIEHLHLIQDHYGCLSAAHLAALAAEMKMALTEVYEVATFYAHFDVVKEGALP
                     PPPITVRVCDSLSCAMAGAERLLKELPGRLGKDVRVVRAPCMGACDRAPVCAIGHVQT
                     FKATADSVTAAVKQKPHAHAWKPDVDFAACQKSGGYALLKSCLTDKRTRDELIKIVSD
                     AELRGLGGAGFPTGRKWALVRAEPAPRMFTVNADEGEPGTFKDRYYLERDPHRFIEGV
                     LIAAWVIEARETYIYIRDEYPEL"
     gene            1074..1967
                     /locus_tag="VFL49_00815"
     CDS             1074..1967
                     /locus_tag="VFL49_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7910549.1"
                     /translation="MIDKLEFILALAREKHFGRAAETCGVTQPTLSAGVKQLEEQMGV
                     LLVNRGSRFQGFTPEGQRVLEWARRIVGDSRTMREEINSLRLGLSGRLRLAAIPTALA
                     MVAALTTPYREKHPNVQFTIYSRTSIEVLELLDNLEIDAGITYVENEPLGRVSTVPLY
                     REHYRLLTSAEAPLGNRETVTWSEVAQVPLCLLTPDMQNRRIIDRLLKSAGGESRPTL
                     ESDSMILLYSHVRTGRWASVMPAKIADTLGLTDTIRAIPITQPKAVQTIGLVVPQREP
                     MTPATAALVTEARRLAPQLSG"
     gene            complement(1978..3198)
                     /locus_tag="VFL49_00820"
     CDS             complement(1978..3198)
                     /locus_tag="VFL49_00820"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET7910550.1"
                     /translation="MATSTASNRASALAESRSLLDDLISPVAAATAKHRLRIIDRVTD
                     LFAAGSRSYSDEQISLFDDVLQRLATDIEVKARAKLAHRLSVIDNAPPVLIRSLAFDN
                     AIEVAEPVLIFSERLTDDDLVENATTKGQDHLYAIAQRLKLSEKVTDVLVERGDQRVV
                     HKVVKNKGARFSLAGYGKLTTRARYDRKLTLALGERSDIPRQYFLKLLEAASSKVRAR
                     LEASNPQMVAAIRDTVDDVATAMQREVRKGSHAHAVAVREAKRRFNVRPITEASVHGP
                     AHAQDFEKTVVALARLGVFPVDLVERALLDEGEDMLLLLAKAAGCSWTTAREMLVMYA
                     ANRNLQPDDITVAFERYRKLSQRTARNIVTFYERRMKLRAAANKKNAHGKRAKDGAED
                     ETLVLLPSPTIAPA"
BASE COUNT          647 a         1005 c         1073 g          695 t
ORIGIN      
        1 agctcggggt attcgtcgcg gatgtagata tatgtctccc gcgcctcaat cacccaggcg
       61 gcgatcagca ccccttcgat gaacctgtgc ggatcgcgtt caaggtaata gcgatccttg
      121 aacgtgccgg gctcgccctc gtcggcgttg acggtgaaca ttcgcggcgc aggctcggcg
      181 cgcacgagtg cccatttccg tccggtcgga aagcccgcac cgccgaggcc gcgcagttcc
      241 gcatcgctca cgatcttgat cagctcatcg cgtgtccgct tgtcggtcag gcaacttttc
      301 agaagcgcgt agcccccgga tttctgacag gcggcaaaat cgacatcggg cttccacgcg
      361 tgtgcgtgcg gtttctgctt gacagcggcg gtcacagagt cggccgttgc cttgaatgtc
      421 tggacgtgac cgatcgcgca aaccggtgcg cgatcgcacg cgcccatgca gggcgcgcgg
      481 acaacacgca cgtccttgcc gagcctgccg ggcaattcct tcagcaatcg ctcggcgccg
      541 gccattgcgc acgacaatga atcacagacg cgtacggtga tcggtggcgg cggcaacgca
      601 ccttccttga ccacgtcaaa atgtgcgtaa aaggtcgcga cttcgtagac ttcggtgagt
      661 gccatcttca tctcggcggc gagcgcagcg agatgtgcag cggagaggca gccgtaatga
      721 tcctgaatca gatgcagatg ttcaatcagc agatcgcgcc ggcgcggcct gtcggtcaga
      781 agcgcgcgaa tttcctcgag ggcttgcgga tcgacttgtc ggcccttggg cgtgcgcggc
      841 ggacggcggc ggccctttcc attggccgca gttgcaggct gattttgaat attcatagcg
      901 ttcgcgtttc ctcgggacac gatttgaagc tgttccagag ttcagacaat cccttgctcg
      961 gtatgggttg atagagaata tctatcggca gtatagcaat tggtgcttat ttttttcgcg
     1021 atccggcagc ggtccaggtt atataaatgt gctgactgaa agggcttaac acattgatcg
     1081 ataagctcga attcattctc gctttggcgc gtgaaaagca ttttgggcgg gccgcggaaa
     1141 cgtgcggggt gacgcagccc acgctgtccg caggcgtgaa gcaattggaa gagcaaatgg
     1201 gcgtcctgct cgtcaaccga gggtcgcgct ttcagggctt taccccggaa gggcagcggg
     1261 tgctcgaatg ggcgaggcgc attgtcggcg acagccgcac gatgcgggag gagataaatt
     1321 cattgcggct cggcctttcc ggccggttgc ggctcgccgc gattccgacg gcgctggcga
     1381 tggtggcggc gctgaccacg ccgtaccggg aaaagcatcc caatgtccaa ttcacaatct
     1441 attcccgcac ctcgatcgaa gtgctcgagc ttcttgacaa cctcgagatc gatgcaggga
     1501 tcacctatgt cgaaaacgag ccgctcggcc gcgtcagcac cgtgccgctc tatcgcgagc
     1561 attaccggct gctgacgtca gccgaagccc cgctcggcaa ccgtgaaaca gtgacgtggt
     1621 cggaggtggc gcaggtgccc ctgtgtcttt tgacgccgga catgcaaaac cgccgcatca
     1681 ttgatcggct gctcaagagt gcgggaggtg agtcccggcc cacgctggaa tcggactcga
     1741 tgatcctgtt gtactcgcac gtccgcaccg gccgctgggc gagcgtgatg ccggccaaaa
     1801 tcgcggacac gcttggcctg accgatacga ttcgtgcaat tccgatcacg cagccgaagg
     1861 ccgtgcagac gatcggcctg gtggttccgc aacgcgagcc gatgacgcca gccacggccg
     1921 cattggtgac ggaagcgcgc cgcctggcgc cgcaattgtc gggttagccg atcagagtta
     1981 cgccggtgcg atcgtcggtg acggcagcag gacgagcgtt tcatcttctg cgccgtcttt
     2041 tgcccttttc ccgtgtgcgt tctttttgtt tgccgccgca cgcagtttca tgcgccgctc
     2101 gtagaaggtg acgatatttc gggcggtccg ttggctgagc ttgcggtagc gctcgaaggc
     2161 gaccgtaatg tcgtcgggct ggaggttgcg gttcgccgcg tacatcacca gcatttcgcg
     2221 cgcggtcgtc cacgagcaac cggctgcctt ggccaaaagc agcagcatat cctcgccttc
     2281 gtccagaaga gcgcgctcga ccaggtcgac cgggaaaacg ccaaggcggg cgagtgcgac
     2341 cacggtcttc tcgaaatcct gtgcatgggc cgggccgtga acgctggcct cggtgatggg
     2401 ccggacatta aaacgacgct tggcttcgcg aacggcgacg gcatgcgcgt gggacccctt
     2461 gcggacctcg cgttgcatcg cggtcgcgac atcgtcgacc gtatcgcgga tcgcggcgac
     2521 catttgcgga ttggaggctt caagcctggc gcgaaccttc gacgaggccg cttccagcaa
     2581 cttgaggaaa tactgccgcg gaatatcgct gcgctcgccg agcgccaaag tgagcttgcg
     2641 gtcatagcgg gcacgggtgg tcagcttgcc gtagccggca agtgaaaacc gcgccccttt
     2701 gttcttaacg actttatgga cgactcgctg atcgccgcgt tcgaccagga catcggtcac
     2761 cttttcgctc agcttcaggc gctgggcgat cgcgtagagg tgatcctggc ctttggtcgt
     2821 ggcgttttca acgagatcgt cgtcggtcag ccgttccgag aaaattagaa cgggttccgc
     2881 cacctcgatc gcattgtcga acgcgagcga gcgaatgagc accggtgggg cattgtcgat
     2941 gaccgacagc cggtgagcga gcttggcgcg cgccttgacc tcgatgtcgg tggcaagacg
     3001 ctgcaatacg tcatcgaaca atgaaatctg ttcatcggaa tagctgcgcg acccggccgc
     3061 aaacaggtcg gtgaccctgt caatgatcct gagccgatgc ttggcggtcg cggcggcaac
     3121 cgggcttatc aggtcatcga ggagcgaacg gctttctgca agcgcgctgg ccctgttgga
     3181 tgcggtgctc gttgccatgg caaattcttt gcaactcaat gaaagtaagg ccgcccccgg
     3241 ccggtttctg cggccggagc gggtaggacg ctaccgcaaa ggtgcgaaaa ctcgctgtac
     3301 gagcccggtc aaaaaaatca aaaaatttta ggaaatttgt acacaactgc ggcgcgaccc
     3361 gcgtgccgcc aatgcaaaaa gggcccgatt tctcgggccc ttttgattgc cgcagcgcgg
//
LOCUS       DASSRL010000039         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_71193, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000039 DASSRL010000000
VERSION     DASSRL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71193"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFL49_00825"
     CDS             complement(<1..737)
                     /locus_tag="VFL49_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910551.1"
                     /translation="MFDQTTDALEWKKLLALLAGHARSARGAALARSGVLSSDVGEAR
                     RLQELTTEMTRLQSGPDPMPVLAFPDVEESLGRARKGAVLETHELRDHALVLALWDDV
                     SRFLARHANDAPTLVAAASPLTAGQELRGMRAALDASIQPDGAVSESATPELRRLTHH
                     ANELKQEIRHRLEDILHSQRYEEALQERYFAEREGRYVVPVKIDMQRRVPGIVHDVSA
                     SGATVFLEPRELIDLNNAIKVADREIDR"
     gene            998..2011
                     /gene="gap"
                     /locus_tag="VFL49_00830"
     CDS             998..2011
                     /gene="gap"
                     /locus_tag="VFL49_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7910552.1"
                     /translation="MAIKVGINGFGRIGRNVLRASLGDPELQFVAINDLTDAKTLAYL
                     LKYDSVHGTLKGSVEAKDDQILVDGKPIKVLAVKDPKELPWKDLNVDVVLESTGRFTD
                     REGAGKHLSAGAKQVIISAPAKDPDITIVLGVNEDKFDPKTHHIISNASCTTNCLAPV
                     AKVLLETFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VLPQLKGKMDGLAIRVPTPNVSLVDLTVETEKDCDVAAVNEAFRKAANGALKGILHYS
                     EDPIVSIDQKGDDHSATVDAPLTTVIDKRLVKVTAWYDNEWGYSCRVRDLIKVLAAKS
                     KSR"
     gene            2075..>3203
                     /locus_tag="VFL49_00835"
     CDS             2075..>3203
                     /locus_tag="VFL49_00835"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7910553.1"
                     /translation="MHKQTIDDISLSGKRVIIRADFNVPLDDSLQITDDTRIRSTLPT
                     INRVVDDGGKAIVCSHLGRPKGTFDPKFSLAPVAKRLGRLLGKDVIFAPDCIGPNIES
                     LVAKMKPGDVMLLENLRFHSGEEKNDEAFSKALAALGDVYINDAFGAAHRAHASTVGI
                     TKYVPVAAAGFLLKKEIEYLEGAVANPVRPFVAILGGAKVSGKIGVIENLGKRVDKVI
                     IGGGMAFTFLKAKGLEIGNSLVENDMLDFAKGIEDHAFSRGVKFYLPVDCVVAASREP
                     GAETKIVPVQEIPKGWYALDIGPASVKLFGLAVQNAKTILWNGPMGVFEVDAFARGTL
                     SIAHAIADAYALTIVGGGETAMAIHRAGESENISFISTGGGA"
BASE COUNT          682 a         1005 c          914 g          602 t
ORIGIN      
        1 cgatcgattt cacgatccgc gaccttgatg gcattgttca gatcaatcaa ttcacgcggc
       61 tcgagaaaga cggtggcgcc gctggcggac acatcgtgca caatgccggg gactcgccgc
      121 tgcatgtcga tcttcacggg cacgacataa cggccttcgc gctcggcaaa gtagcgttct
      181 tgaagcgctt cttcatagcg ctgtgaatga aggatgtctt cgaggcgatg gcggatttcc
      241 tgcttaagct cattggcgtg atgtgtgagt cggcgcaact ccggggtcgc cgactcgctg
      301 accgcgccgt ccggctgaat ggaagcgtcc agcgcagccc tcatgccgcg caactcctgg
      361 ccggccgtta gcggcgacgc agccgcaaca agggtgggcg catcgttcgc atgtctggca
      421 agaaaccggg agacgtcgtc ccaaagcgcg aggacgagtg catggtctcg cagttcatgc
      481 gtttcgagca cggccccctt gcgggcccgt ccgagcgatt cttcgacatc gggaaatgcc
      541 aacacgggca tgggatccgg cccgctctgg agcctcgtca tttccgtcgt caactcctga
      601 agacgccgcg cttcaccaac atcggacgac aggacgcccg accgggccag tgccgctccc
      661 ctcgccgaac gcgcgtgacc ggccagcaac gccaggagtt ttttccattc caatgcatcg
      721 gtcgtctgat cgaacacagg agtcctcaca ccaacgatgg ctgctgtccg gccggcccag
      781 gcaaaaactc tagcgaaccg gctatctgac acgcaagccg gcctacggcg tagtggctcc
      841 agcccatggc cgcagctcac ctgattcgag acgtctgaaa tcggtttgac aacgcggagt
      901 taccactttt gtatcgcttg actcaggggg gcgaccgccg atactatcgc catcgtcgca
      961 ggggaaaaac tacgtttttc gaagggacaa ctccaccatg gctatcaaag tcggcatcaa
     1021 cgggttcgga cggatcgggc gcaacgtctt gcgcgcctcc ctcggcgacc cggaacttca
     1081 gttcgtcgcg atcaatgatt tgaccgatgc caaaaccctg gcctacctgc tgaagtacga
     1141 ctcggtgcac ggcaccctca agggcagcgt cgaagccaaa gacgatcaga tcctggtcga
     1201 tggcaaaccg atcaaagtgc tggccgtcaa ggatcccaag gagttgccct ggaaagatct
     1261 caacgtcgac gtcgttctcg aatcgacggg gcgctttacg gaccgcgaag gagcgggcaa
     1321 gcacctttcc gccggcgcca agcaagtcat catctccgct ccggcgaaag acccggatat
     1381 caccatcgtg ttgggcgtca atgaagacaa gttcgacccg aaaacgcatc acatcatctc
     1441 caatgcctcc tgcacgacca attgcctggc gcccgtcgcc aaagtgctct tggagacgtt
     1501 cggcatcaaa catggcgtga tgaccaccat tcactcgtac accaacgatc aacagctgct
     1561 cgacctgccg cacaaagacc tgcgtcgggc ccgggccgcc ggcatgtcga tgattccgac
     1621 cagtaccggt gcggccaagg cattacatct cgtgttgccg caattgaaag gcaagatgga
     1681 tgggctcgca atccgggtgc cgaccccgaa cgtctcgctg gtggatctga ccgtggaaac
     1741 cgaaaaagat tgcgacgtcg cggcggtcaa cgaagccttc cgcaaagctg caaacggggc
     1801 gctaaagggc attctgcatt actccgaaga tcccatcgtg tccatcgatc aaaaaggcga
     1861 cgatcactcc gccacggtcg acgccccctt gacgacggtc atcgacaagc ggctggtcaa
     1921 ggtcacggcg tggtacgaca acgagtgggg ctattcctgc cgcgtgcgcg acctgatcaa
     1981 ggtccttgcg gcgaaatcca agagccggtg aaccctaacc ttcccgcgtg cttcgtcagc
     2041 ccgctccacg ctgacccata ggacacccat gaacctgcat aagcagacga tcgacgacat
     2101 ctcgctgtcc gggaagcgcg tgatcatccg cgccgatttc aacgtgcctc tcgatgattc
     2161 tctgcaaatc acggacgaca cccgtatccg ctcgacgctc cccacgatca accgcgtcgt
     2221 cgacgacggc ggcaaagcca ttgtctgctc gcacctcggc cgcccgaagg gcaccttcga
     2281 tcccaaattc agcctggcgc ccgtcgcgaa acggcttgga cggctgctcg gcaaagacgt
     2341 gatctttgcg ccggactgca tcggcccaaa catcgaaagc ctcgtggcca aaatgaagcc
     2401 tggcgacgtc atgttgctcg aaaatcttcg gtttcattcc ggcgaagaaa aaaatgatga
     2461 ggccttttca aaagcgctgg cggcactggg cgatgtctac atcaacgatg ccttcggcgc
     2521 cgcgcaccga gcccacgcgt ccaccgtcgg catcacgaag tacgtgccgg tcgccgccgc
     2581 cggttttctg ctgaagaaag aaatcgagta cctggagggg gcggtcgcaa atcccgtgcg
     2641 gccgttcgtc gccattctcg gcggcgccaa ggtctccggt aaaatcggcg tgattgagaa
     2701 tctcggcaag cgggtggaca aggtcattat cggcggcggc atggccttca cgttcttgaa
     2761 ggcgaaaggc ctcgagatcg gcaactcgct ggtggaaaac gacatgcttg atttcgcgaa
     2821 gggcatcgaa gatcatgcgt tctcccgcgg cgtgaaattc tatctgccgg tcgattgcgt
     2881 cgtcgccgcg agccgtgagc ctggggccga gaccaagatc gtccccgtcc aggaaattcc
     2941 caaaggctgg tacgcgctcg acatcggtcc tgcctccgtc aaattgtttg gattagcagt
     3001 gcaaaacgcc aagaccattc tctggaacgg ccctatgggt gtgttcgaag tcgacgcatt
     3061 cgcgcgcggc acgttgtcga tcgctcacgc gatcgccgac gcgtatgcct tgaccatcgt
     3121 cggcggcggt gaaaccgcca tggccatcca tcgagcgggc gaatctgaaa acatttcctt
     3181 tatctccacc ggtggcggcg cgg
//
LOCUS       DASSRL010000040         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_403668, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000040 DASSRL010000000
VERSION     DASSRL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403668"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1731
                     /locus_tag="VFL49_00840"
     CDS             184..1731
                     /locus_tag="VFL49_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7910554.1"
                     /translation="MATTYQFGEFCLDLEAGILSRGAQPSMLGQRAVALLRLLLDHTG
                     AAVSKEALIEAAWPGLAIADNNLTVQIATLRRLIEQAGGEARWIETLPRRGYRYVGPA
                     VARNESQAEPSNLAPLALPDKPSVAVLPFSNLSGDPEQGYFSDGMVDDIITGLSRIKW
                     LFVIARNSSFVYRGRTVDVKQVGQELGVRYVLQGSVRKLSDRVRINGQLADAATGTHV
                     WAERYDRRFDDIFALQDEIALSVVGAIEPSLRQAEVERVARKRPDSLDAYDLVLRAQS
                     DIFSGMPERSAKALVLLDRALALDPAYALAHGFAAMGHHNRFLRAGLREEDRAASIRH
                     ARAAIANGRDDALALTFAGFSIGMDAHDRTAAFTAFEAALAISPSTALTHILGSVILS
                     WTGEADRAIEWAERGLRLSPFDPWAFAAFGSLALGHFLRGRSEAAIHHAYRAVQSNPV
                     HSINYVLLAAMLANAGRIEEARTAAVRVLELHPTFRYGRQFSGVDCAPKLATALGDML
                     GALGLPE"
     gene            complement(1803..>2386)
                     /locus_tag="VFL49_00845"
     CDS             complement(1803..>2386)
                     /locus_tag="VFL49_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7910555.1"
                     /translation="IVAVPIGVLAANAIGFAPPLKADRRAAIAALPMALYDKVAVGFS
                     RHAIEAPADARVTALTAHGRVLQVIAQPGGRNGAVVLVDGDEARQLEANGPSAARAWA
                     LSALAEIFGKTLRSAFAGAVETRWGQDVYSRGAWSVARKGQVGERDRIVLAQPHDGRV
                     FFAGEATEGGDLAGAHSSGVRAATEALTLLARH"
BASE COUNT          410 a          809 c          740 g          427 t
ORIGIN      
        1 gtgaccagaa aacacgactt ctttaccctg acccggcttc cggacaatcg cctttgcgct
       61 cattctgggc ctccattgcg tggcatgcgg ccacgctgca actcgagagg atgatccttc
      121 caccccctga agtcgtgaat ccgctcctaa aaagttgcaa aatgattccc aagcaaccgg
      181 cccatggcga ccacgtacca attcggtgag ttctgcctcg acctggaggc gggaattctg
      241 tctcgcggcg cccaaccctc gatgctcggg caacgggcgg ttgcgctgct ccgactgctc
      301 ttggaccaca cgggggcagc ggtctcgaag gaagcgctga tcgaagcggc atggcccggg
      361 cttgccatcg cagataacaa cctgacggtt caaattgcga cactgcgccg tctcatcgag
      421 caggcaggtg gggaggcgcg atggatcgag acattgccac gccgtggcta ccgctatgtc
      481 gggcccgcgg tagcgaggaa tgaatcacag gccgagccct cgaacctggc gccgctcgcg
      541 ctgcccgaca agccgtccgt ggcggtgctg ccgttttcga atttgagcgg agatcccgag
      601 cagggctatt tttccgatgg catggtcgac gacatcatca ccggcctgtc gcggatcaag
      661 tggcttttcg tcatcgcgag gaattcgagc tttgtctacc gcggccgaac agtcgacgtg
      721 aagcaggtcg ggcaggaact cggggtgcgc tacgtgcttc agggcagcgt tcgcaaactg
      781 tcggatcgcg tgcggatcaa cgggcagctc gcagatgcgg caaccggaac gcatgtctgg
      841 gccgaacgat acgatcgcag gttcgacgac atttttgccc ttcaagatga aatcgcccta
      901 tcggtcgtcg gcgccatcga gccgagcttg cgccaggccg aagtggagcg cgtcgcgcgc
      961 aagcgccccg acagtctcga cgcgtatgat ctcgtcctgc gggcgcagtc cgatattttc
     1021 tccggcatgc ccgaacgctc tgcaaaggca ttggtgctgc tggatcgtgc gctcgcgctg
     1081 gatcccgctt acgcgctggc gcacggtttc gccgcgatgg gccatcataa ccgattcctt
     1141 cgcgccggcc tgcgcgagga agacagagcg gcctcgatcc gccacgctcg cgcagcgatt
     1201 gccaatggcc gggatgacgc cctcgccctg acctttgccg gattttccat cggcatggat
     1261 gcgcatgatc gcacggccgc cttcactgcg ttcgaggcgg cattggcgat aagcccatcg
     1321 accgccctca cccatattct cggcagcgtc atcctcagtt ggaccggaga ggctgaccgc
     1381 gcgatcgagt gggcggagcg aggcctgcgc ctcagcccat tcgatccttg ggcctttgcc
     1441 gccttcggct ccctggcact cggccatttc ctgcgcggcc gctccgaggc cgcaattcat
     1501 cacgcctaca gagccgtcca gtccaacccg gtccacagca tcaactacgt gctgctggcg
     1561 gccatgctgg cgaacgccgg acgcatcgaa gaggccagga cagcagcagt acgcgttctg
     1621 gaattgcatc cgacgttccg ctacggccgg caattttcag gcgtcgattg cgcgcccaag
     1681 ctcgccacgg ccttgggcga catgctcggc gccctcggac ttccggaatg aagagcagct
     1741 cgaacgaggg caatgtccgt cttggccttc tgatgaaccg cacttatttg ggtttttcga
     1801 ttctaatgtc gcgccagcaa ggtcagcgcc tccgtcgccg cccgtacccc cgatgaatgc
     1861 gcaccggcaa ggtcgccgcc ctcggtcgcc tctcccgcga agaagacacg tccgtcgtgc
     1921 ggctgggcca gcacgatgcg gtcgcgctcg cccacctggc ccttgcgcgc gaccgaccag
     1981 gcgccgcggg aatagacgtc ctgcccccag cgtgtttcga cggcgcccgc gaaggcggac
     2041 cgaagcgtct tgccgaagat ctcggccagc gccgacagcg cccacgcgcg cgccgcgctg
     2101 ggtccgttcg cctcgagctg acgcgcctcg tcaccatcga ccaggacgac agcgccattg
     2161 cgtccgcccg gctgtgcgat gacctgcagc acgcggccgt gcgccgtgag cgccgtcacc
     2221 cgggcatcgg ccggcgcttc gatcgcatgg cgcgagaagc cgaccgcgac cttgtcgtag
     2281 agcgccatcg gcaaggccgc gatcgccgcg cgcctgtccg ctttcagggg cggggcgaag
     2341 ccgatggcat tcgccgccag cacgccgatc ggcacggcga cgatcg
//
LOCUS       DASSRL010000041         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1225988, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000041 DASSRL010000000
VERSION     DASSRL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225988"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /gene="gloB"
                     /locus_tag="VFL49_00850"
     CDS             complement(<1..717)
                     /gene="gloB"
                     /locus_tag="VFL49_00850"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HET7910556.1"
                     /translation="MPAEIRLFRCRSDNYGVLLHDAESGTTASIDAPEAAPIEAALKA
                     SGWKLTDILVTHHHADHTDGIRALKDKYKCRVVAPATEASKIPAVDETVREGDKVNVG
                     TLSANVIETPGHTLGHIAYWFHGDKVAFVGDTLFSIGCGRVIEGTPEQMWRSLVKLRD
                     LPNDTEFYCGHEYTAANVKFARTIEPNNKALAAREAEANKQLARNEATIPATIGEEKQ
                     ANPFLRADVPEVAAGIGMAGK"
     gene            835..1566
                     /locus_tag="VFL49_00855"
     CDS             835..1566
                     /locus_tag="VFL49_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7910557.1"
                     /translation="MSIDVVDLRNFYGQRLGVVARRFIGRGIRSLWDDTTGQRVLGIG
                     YATPYLGLFREEAERCLALMPAGQGVVKWPTMRPALSALVYESELPLADSAVDRVMLV
                     HALEMTNDPAALLREVWRVLASGGRLLAVVPNRRGVWARMDTTPFGHGRPYSRSQINQ
                     LLRETWFTPTGWSEALYVPPVERGWFLRTAVAWERTGATLSAPFAGVHLVEATKQVYR
                     AIPARREKRKLVPALEPVLAPSPGR"
     gene            complement(1583..2404)
                     /locus_tag="VFL49_00860"
     CDS             complement(1583..2404)
                     /locus_tag="VFL49_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HET7910558.1"
                     /translation="MRNGQKRMRGRGNHRKSQNPLTRVYESNGPDVKIRGTASHIAEK
                     YIQLARDAQGSGDPVAAENYYQHAEHYFRLIAAAQEQFRQQNPYYNQQPHQQGAPGGQ
                     PQGGDDAFDSDDDDNQSYMGQNEPSYAPREPQQQPYLPRDAQPFPPRDQQVQNRPHHQ
                     QQPYPQPNQGSHEGGQDVGALPAFITGGGQPQQNSGPNGYDQGDRGQDRGQDRGQDRN
                     QDRGQDRFGHRRRRRHRGGGYRPDRQDFGGGHQQNVAPQPAQDDGGAGDEPRQPE"
     gene            complement(2895..>3567)
                     /gene="prmC"
                     /locus_tag="VFL49_00865"
     CDS             complement(2895..>3567)
                     /gene="prmC"
                     /locus_tag="VFL49_00865"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7910559.1"
                     /translation="RRLRREPVSRILGQKEFWSLPLAVTPDVLVPRPETETVVEAALD
                     FVVRGGLRLEKLRVLDIGTGTGALLLALLNELPNALGTGTDVSSAALRVAHANAVHCR
                     LNSRCNFVACDIATGVAGPFDLVVSNPPYIARGEIVALAREVRDYDPQVALDGGRDGL
                     DAYRVIANDAKRILAPGGRLFVELGAGQEGQVRALFTKAGLNPGGPRKDLAGIPRVLG
                     ASFAP"
BASE COUNT          569 a         1132 c         1107 g          759 t
ORIGIN      
        1 ttttcccgcc atgccgatgc cggcggcgac ttcgggcaca tcggcgcgca ggaacggatt
       61 ggcttgcttc tcctcgccga tggtcgctgg gatggtcgct tcgttccgcg caagctgctt
      121 gttggcctcg gcttcgcgcg cagcaagcgc cttgttgttg ggctcgatcg tgcgcgcgaa
      181 tttgacattc gcggcggtgt attcgtgccc gcaatagaac tcggtgtcgt tcggcaaatc
      241 gcgcagcttg acgagcgaac gccacatctg ttccggcgtg ccctcaatca cgcggccgca
      301 gccaatcgag aacaatgtat cgccgacgaa agcaaccttg tcgccgtgaa accagtacgc
      361 aatgtggccg agcgtatggc ctggggtctc gatgacgttg gcggacagcg tgccgacgtt
      421 gaccttgtct ccttcgcgca cggtctcgtc gactgccgga atttttgaag cctcggtggc
      481 gggtgccacc acgcgacatt tgtatttgtc cttcagcgcg cggatgccat cagtgtggtc
      541 ggcatggtga tgcgtgacca gaatatccgt gagtttccag ccgctcgcct tcagcgcggc
      601 ctcaatcggt gcagcttccg gcgcatcgat cgaagcggtc gtgccgcttt cggcgtcgtg
      661 cagcaatacg ccgtagttat cggatcggca gcgaaacagg cggatttccg cgggcatgag
      721 cttggtcctt tggcactctg caatgggtta accgtatcac gcgggtgaat gaagttgcac
      781 gcccgtgtgt tcccgcgttg ttagcgcttt accatttcgt ggtagtttgc ccgcatgtcg
      841 atcgacgtcg tggacctgcg gaatttttac ggccagcggc tgggcgtcgt ggcgcgccgg
      901 ttcatcggcc gcggcatccg ctctctgtgg gatgatacca ccggtcagcg tgtgctcggc
      961 atcggctatg cgacgcctta tctcggcttg tttcgcgaag aggccgagcg ctgtcttgcg
     1021 cttatgcccg ccgggcaggg ggtggtgaaa tggccgacaa tgcggccggc gctctccgcc
     1081 ctcgtctacg agagcgaact gcccctcgcc gattccgccg tcgaccgcgt gatgctggtg
     1141 cacgcgctgg agatgactaa cgatccggca gcgctgctgc gcgaagtgtg gcgagtgctc
     1201 gcctcgggcg ggcgcctgct tgccgtcgtg ccaaaccggc gcggcgtctg ggcgcgcatg
     1261 gatacgacgc cattcggtca cggacgcccg tactcgcgca gccagatcaa ccagctcctg
     1321 cgcgagacgt ggttcacgcc aaccggctgg agtgaagcgc tttacgttcc gccggtcgaa
     1381 cgcggctggt ttctgcggac cgcggtggcg tgggagcgca ccggcgcaac gctgtcggcg
     1441 ccgttcgctg gcgtacatct ggtggaggcg accaaacagg tctatcgcgc gataccggct
     1501 cgccgcgaaa agcgcaagct ggtgccggcg ctggagccgg tgctggcgcc gtcacccggc
     1561 cgatgagcgc cgcttcagat tcctattctg gctgacgtgg ctcgtcgccg gcgccgccgt
     1621 catcctgcgc cggctgcggc gcgacgttct gctgatgccc gccgccgaaa tcctggcggt
     1681 ccggacgata tccgccacca cgatggcgcc ggcgccgccg gtgtccaaat cgatcctgcc
     1741 cgcgatcttg attccgatcc tggccccgat cctgtccgcg atcctggccc cgatcaccct
     1801 ggtcatagcc gttcggcccg gaattctgct ggggctgacc gccgccggtg atgaatgccg
     1861 gaagagcgcc gacatcttgt ccgccttcgt gactgccctg gttcggctgc ggatagggct
     1921 gctgctgatg atgcgggcga ttctgaacct gctgatcacg cggcggaaac ggctgtgcat
     1981 cgcgcggcag atacggctgc tgctgtggct cacgcggcgc gtagctcggc tcgttttggc
     2041 ccatgtagga ttgattgtcg tcgtcgtcgc tatcgaaggc atcgtcgccg ccctgcggct
     2101 ggccgcccgg tgcgccctgc tgatgcggct gctgattata gtacgggttc tgctgacgaa
     2161 attgctcctg cgctgcggcg atcaggcgga agtagtgctc ggcgtgctga taataattct
     2221 cggcggccac cggatctccc gacccctgcg cgtcgcgcgc gagctggatg tatttttccg
     2281 cgatgtgtga agcggtgccg cggattttga cgtcgggacc gttcgattcg taaacgcgcg
     2341 tgagcggatt ctggcttttg cggtggttgc ctcgcccccg catgcgcttc tgaccgtttc
     2401 tcatgatgtg cctgttcgtc gacctttcac cctgtcgcgg catttgcgcc gaacgctcag
     2461 ctcaatcagc aagtccaaga aatctcattg cgcggccgga cttgcgtgat atccgcgcca
     2521 atgatcgaaa tgtcgtgtcc tatatcgcga tagtcgcagc aaccccattg ccgcggtctg
     2581 agccgcagcg ttgccaacct ctcgttacgc gcatcgctct acgtttcgaa aattgatgcg
     2641 cccaaccggt gaagcattca aacccgtctc tggcgatttc gtctgccgtc tcttcgaagc
     2701 cggctcttcg tcatccgcct cgcgccgcac ctcacagagc ctctcgctcc ggggtttttg
     2761 gcgatgcaac cgtcgcgggt tgattccgta accggaagct agtcgtttcc gagccataat
     2821 ccaagtgctt ttttcaatga ataatcgggc tctgcatttt catgcggtgg gaatgcattg
     2881 tgctcatatc gatctcatgg agcgaaactc gcgccaagca cgcgcggaat tccggccaga
     2941 tccttgcgcg ggccgcccgg gttcagtccg gccttggtaa acagcgcgcg gacctgtccc
     3001 tcctggcccg cgcccaactc gacgaacaat cgtccgcccg gtgccagaat acgttttgca
     3061 tcatttgcaa tgacccgata ggcgtcgagg ccgtcccggc cgccatcgag cgcaacctgc
     3121 ggatcgtagt cgcggacctc gcgcgccagc gccacgatct cgccgcgagc gatataggga
     3181 ggatttgaca cgaccagatc gaaagggcct gcaacgccgg ttgcgatgtc gcaagcaaca
     3241 aaattgcaac gggaattcag tcgacaatgt accgcattgg cgtgcgcgac gcgcaatgcg
     3301 gctgaactca cgtcggtgcc tgttcccaga gcgttcggca actcgttcaa cagtgcgagc
     3361 agcagcgcgc cggtgccggt gccgatgtcg agcacccgca acttttccag gcgcagacca
     3421 ccgcgcacca cgaaatcgag cgccgcttcc acaaccgtct cggtttccgg ccgcggcacg
     3481 aggacatcgg gcgtcactgc cagcggaaga ctccagaatt ctttttgtcc cagtattcgc
     3541 gacaccggtt cgcgccgcag ccggcgt
//
LOCUS       DASSRL010000042         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_543821, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000042 DASSRL010000000
VERSION     DASSRL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543821"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1208
                     /locus_tag="VFL49_00870"
     CDS             66..1208
                     /locus_tag="VFL49_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7910560.1"
                     /translation="MRKISTLGVVLSIAMATAGTATAQIKFGVGGPITGPSAATGAQM
                     KNGVDQAAADINAAGGILGQKIVVSYGDDVSDPKQGVSVANKFSADGVKFVIGHYNSG
                     VTIPSSEVYQENGILQITPASTNPKVTERNMWNIFRVCGRDDQQGKVAGEYIVKHFKG
                     KKIALVHDKTTYGKGLADETKKAISKGGMKEVLYEGINTGEKDFSALVSKIKQSGADL
                     IYWGGLYTEGGLIVRQMRDQGVKAPLMGGDGITSDEFAAIGGPGVEGTLMTYGPDPRN
                     KPEAKKVVEEFRAKKFEPEAYTLYSYAGVQIIKQAAEAAKSLDPKKVAEKMHSGMHFK
                     TVLGDISYDKKGDITKLDYVMYIWKKDGSGKITYVECPNSDCSKVK"
     gene            complement(1258..1992)
                     /locus_tag="VFL49_00875"
     CDS             complement(1258..1992)
                     /locus_tag="VFL49_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7910561.1"
                     /translation="MHADVTYLAAVVAGLVSFLSPCVLPLVPPYLVFLAGTSLERFAD
                     REPEPRVKRETVAAAALFVLGFSTVFVALGASASAIGSLIRAYSGPLATVAGIVIIIM
                     GLHFLGVTRIALLHRQKRLEVVKPVGLWGAYIIGLAFAFGWTPCIGPILAAILAVAAS
                     EQTVTKGASLLAVYSLGLGIPFVIAAFAIEPFAAFLSRFRNYLHRVEQIMGALLILTG
                     IAFLTGSINQMSVWLLETFPVLGKIG"
     gene            2128..3114
                     /locus_tag="VFL49_00880"
     CDS             2128..3114
                     /locus_tag="VFL49_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7910562.1"
                     /translation="MRNLITDIPGIRVGHAGDAKLGSGVTAIIFDSAVVASVDVRGGG
                     PGTRETALLDPAQTIEGIDAVVLSGGSAFGLDSASGVQAWLREKGRGFQVREARVPIV
                     PAAILFDLLSGGDKDWGRYPPYRELGYEAAKTAAEDFALGSAGAGLGATTATLKGGIG
                     SASAKTRSGLTVGALAAANAAGSMTIGDGPHFWAAPFEQDKEFGGRGWPATFPADALK
                     FRSKGNLGENTTLAVVATDAKLTKAQAKRLAVMAQSGLSRAVYPVHTPLDGDVVFAVG
                     MGAKPLPDLVFSLSELGMLAANVLARAIARGVYEASALPFNGALQCWRDRFA"
     gene            3152..3364
                     /locus_tag="VFL49_00885"
     CDS             3152..3364
                     /locus_tag="VFL49_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HET7910563.1"
                     /translation="MQSSAVADIRYDHARERLTVTFVTGRVYEYVDVPVEVAASFQSA
                     FSKGTFFNAYIRDRYDFREITPARAS"
     gene            complement(3357..>3658)
                     /locus_tag="VFL49_00890"
     CDS             complement(3357..>3658)
                     /locus_tag="VFL49_00890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7910564.1"
                     /translation="ARDAQPSPVQFDRIMPRARELFPLGERADDRTWMGSRPCFPDSR
                     PVIGRAPQLSGLWLAIGHAHWGLTLGPATGRLISEMMAGETPFCDPTPYRAERFN"
BASE COUNT          801 a         1057 c         1144 g          656 t
ORIGIN      
        1 gcaaactgcc ctgacataag attgccgcag gcagtgtgcc ggctccagtt ggaggggaac
       61 gagagatgag aaagataagc acgctcggtg tcgtactcag cattgcgatg gcgacggcgg
      121 ggaccgcgac ggcgcagatc aaattcggcg tgggcggacc cattaccgga ccaagcgccg
      181 cgacgggcgc acaaatgaaa aacggcgtcg atcaggcggc tgcagacatc aatgctgcag
      241 gcggaatcct cggtcagaag atcgtcgtct catacggcga tgacgtatcc gatccgaaac
      301 agggcgtttc ggttgccaac aaattttcgg ctgacggcgt caaattcgtc atcggccatt
      361 acaattccgg tgtcacgatc ccgtcatctg aggtctacca ggagaacggc attctgcaga
      421 tcacgccggc ttcgaccaac ccgaaagtta ccgaacgcaa catgtggaac attttccgcg
      481 tctgcgggcg tgacgaccag caaggcaagg ttgccggcga gtacatcgtg aaacatttca
      541 agggtaagaa gattgcgctc gtccatgaca agaccactta tggcaagggc cttgccgacg
      601 agacgaagaa agcgatcagc aaaggtggaa tgaaggaagt cctctacgag ggcatcaaca
      661 caggcgaaaa agacttttcg gcgctggtgt ccaagatcaa gcaatccggc gccgatctca
      721 tctattgggg cggcctatat actgaaggcg gcctgattgt gcggcagatg cgcgaccagg
      781 gcgtgaaggc tccgctgatg ggcggcgatg gcattacgtc ggatgagttt gccgcgatcg
      841 gcgggcccgg tgtcgaaggc acgctgatga cctacgggcc ggacccgcgc aacaagccgg
      901 aagcgaagaa ggtggtcgag gaattccgcg cgaaaaaatt cgagccggaa gcctacaccc
      961 tctacagcta cgctggcgtc cagatcatca agcaggcggc cgaagcggcc aaatcgcttg
     1021 atcccaagaa agtagccgaa aagatgcatt caggcatgca cttcaagacc gtgctcggcg
     1081 atatctccta tgacaagaaa ggcgacatca ccaagctcga ctacgtgatg tacatctgga
     1141 agaaagatgg cagcggcaag attacctatg tcgagtgccc gaattccgac tgcagtaaag
     1201 tgaagtaacc ggccttgtca ccggaacagg cccgccattc cggcgggcct tcttttttca
     1261 gccgatcttg ccgagcaccg gaaaagtctc cagcagccac acactcatct ggttgatgct
     1321 gccggtcagg aaggcgatcc ccgtcaagat cagcaatgca cccatgatct gctcgacgcg
     1381 atgaaggtaa ttgcgaaaac gcgagagaaa agccgcgaag ggctcgattg caaatgcggc
     1441 aatgacgaag ggaataccga gtcccagcga atagaccgcg agcaggctcg cgcccttggt
     1501 caccgtctgc tcggatgccg ccacggccag gatggcggca agaatgggcc cgatgcacgg
     1561 tgtccaaccg aaggcgaagg cgagcccgat gatgtacgcg ccccacaggc cgacaggttt
     1621 gacgacctcc aggcgcttct gacggtgcag aagggcgatc ctcgtgacac cgaggaaatg
     1681 caaccccatg atgatgatga cgatcccggc cacagtcgca agcgggcctg agtaggcgcg
     1741 gatcagcgag ccgatcgcgc tggcgctcgc ccccagcgcc acaaacaccg tggaaaagcc
     1801 caagacgaac aatgccgcgg cggctaccgt ttcgcgcttg acgcggggtt cgggttctcg
     1861 gtcggcaaac cgctcaagcg acgtgcccgc cagaaataca agataaggcg gcaccagcgg
     1921 caatacgcag ggagagagaa agctgaccag gccggcgacc actgccgcca ggtacgtgac
     1981 atcggcatgc atccgcccta gatgcgccag cagatacggc ggtggcaagt ggtgccgccg
     2041 ggtctgactt cgaagtgatc gatccgtgaa ccggacgatt gacgggttgc cgcaaatcgt
     2101 ctccgctttc gattacagga ggtgcctttg cgaaatttga tcaccgatat tcccggaata
     2161 agggtcggcc atgcaggcga cgccaaactc ggctcggggg tgaccgcgat cattttcgat
     2221 tcagctgtgg tcgccagcgt cgacgttcgc ggcggtggac ctggcacacg cgagaccgcc
     2281 ctgctcgatc cggcgcagac tatcgaaggc atcgatgccg tcgtgctgtc cggcggatcg
     2341 gcgttcggac tcgattccgc ctcaggcgtg caagcatggc tgcgcgagaa gggccgtggc
     2401 tttcaggtgc gcgaggcgcg agtgccgatt gtgccggctg cgatcctgtt cgatctgttg
     2461 tcgggcggcg acaaggattg gggccgctat ccgccttatc gggagctcgg ctacgaagcc
     2521 gcaaaaaccg cggccgagga tttcgcgctt ggtagtgccg gtgctggcct aggtgcgaca
     2581 accgctaccc ttaaaggtgg catcggatcg gccagtgcaa aaacccgcag cgggctgact
     2641 gtgggcgccc ttgcagccgc caacgcggca ggcagcatga cgattggtga cggcccgcat
     2701 ttctgggccg ccccttttga gcaagataag gagttcggcg gccgcggctg gcccgcgacg
     2761 tttcccgccg atgcgctgaa attccgcagc aagggcaatc tgggcgaaaa cacaacgctc
     2821 gcggtagtcg cgaccgatgc aaaactcacc aaagcgcagg cgaaacggct ggccgtgatg
     2881 gcgcaaagtg gtctatcgcg cgcggtatac ccggtgcaca ccccgctcga cggcgatgtg
     2941 gtattcgcgg ttggcatggg tgcaaaaccg ttgccggatt tggtgttttc tctcagcgaa
     3001 cttggaatgc ttgcggccaa tgtgctcgcg cgcgccatcg cgcggggcgt ctatgaggcc
     3061 tcggctttac cattcaatgg tgcgctgcaa tgctggcgtg atcgctttgc atgacttctg
     3121 aacttcgcaa tgatttatgc gttgtccggt catgcaatcg agtgcagttg ccgacatcag
     3181 atatgaccac gcgcgggagc ggctgaccgt aacgttcgta accggccgcg tctacgagta
     3241 cgtcgatgta ccggtcgagg ttgcggcgtc ctttcagtct gctttctcga aaggtacttt
     3301 cttcaacgcc tatatccgcg accgctacga cttccgcgaa atcacacccg cgcgtgctag
     3361 ttaaatcgct cggcacggta aggcgttggg tcgcagaaag gcgtttcgcc cgccatcatt
     3421 tctgaaataa ggcggccggt cgccggtccc agcgtcaggc cccaatgcgc gtgtccgatg
     3481 gccaaccata gcccggacag ttgcggcgcg cggccgatca ccggcctgga gtcggggaag
     3541 cagggccggc tgcccatcca ggttcggtcg tcggcccgct cgcctagcgg aaaaagttca
     3601 cgtgcccttg gcattatgcg gtcgaactgc accggcgaag gttgtgcgtc gcgcgcag
//
LOCUS       DASSRL010000043         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_491184, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000043 DASSRL010000000
VERSION     DASSRL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491184"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFL49_00895"
     CDS             <1..294
                     /locus_tag="VFL49_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HET7910565.1"
                     /translation="LAKLKFLRGTAFDLFGYTEDRRTERRLIAEYEYLLGEICEFLTP
                     GNHRFAVALAAIPEKIRGYGPVKQRHLAAAKAEEAALREQFLSGSAPLLRAAE"
     gene            528..>2401
                     /locus_tag="VFL49_00900"
     CDS             528..>2401
                     /locus_tag="VFL49_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7910566.1"
                     /translation="MRHKDLGIWQTWTWAQSLDSVRAYAVGLHRLGLKRGETIAIVGA
                     NRPKLYWSVMAAQMLGAIPVPVYADAVADELAYVLAHADVRFAAVEDQEQVDKIQSVL
                     ERVPKLQLMLYDEKRGLRDYDHSRLHSMDDVIEEGRKALANDPSVRAWLDGEIAAGKG
                     SDPSIILYTSGTTGASKGVLLTGERSIKAASDTVAFDKLTEHDVSLAYLPLAWVGDHY
                     LNYAQGLVSGFCTACPESSDTAMADLREIGPSFYFAPPRTLEVLLTRVTIRMEDAGFL
                     KRRMFHYFVGVARKYGERILNHEHVPLTGRLLYRLGDLLVYGPLKNVLGLSRVRVAYT
                     AGEAIGPDLFAFYRSIGLNLKQLYGQTEAFLYLTAQPDGEIYSDTVGRTFPNVDIRIA
                     ENGEVLFKSPGMFAGYFKDDAKTAEVMTPDGYVKTGDAGYFDEKTGHLKIIDRARDVG
                     RLNDGTLFPPKYIENKLKFYPNIREAVAYGDKRDFVAVMLNIDLAAVSSWAERNNVVY
                     GSYQELAGHPLVYDMLEKHVAEVNHSLAQDKMTAGAQIKRFLILHKELDPDDGEITRT
                     LKVRRGFIEERYAPLIKALYDGSKEADISTEVTFEDGRKGVISARVKIRDMKTEPVAA
                     A"
BASE COUNT          497 a          687 c          736 g          481 t
ORIGIN      
        1 ctcgcaaagc tgaagttctt gcgcggcact gccttcgacc ttttcggcta taccgaggat
       61 agacggactg agcgccgctt gattgcggag tacgagtatc ttcttggcga aatctgcgaa
      121 tttctcacgc ccggaaacca ccgttttgcg gtggctctgg cggcgatccc cgagaaaatc
      181 cgcgggtatg gaccggtcaa gcagcggcat ctggccgcgg caaaggccga ggaagcggcg
      241 ttacgggagc agtttttgtc ggggtcagcc cctctcctga gggcggccga atagtgtagg
      301 tccccacgct agtgggcatt cccccatcgg atacttgacc gccttcgggc ggtgttttca
      361 tatcgagaca acaagaccgt ccggacagga cggcgagggg ttaagtgccg cgccagagcg
      421 cggtctcacg ggaggactga gctgtggcgc cgaacgccgc cgcgggtgcg gatacgtttc
      481 ccaagctgct ggtccgcaat gcccaggctt ttgggacgcg accggccatg cgccataaag
      541 atctgggcat ctggcagaca tggacctggg cgcagtcgct cgacagcgtt cgcgcctatg
      601 cggtcggctt gcaccggttg ggccttaagc ggggcgagac catcgctatc gtcggcgcca
      661 atcggccgaa actctattgg tcggtcatgg ccgcacagat gctcggtgct attcctgttc
      721 cggtctacgc ggatgcagtg gcggacgaac tggcctacgt gttggctcac gccgatgtgc
      781 gctttgccgc agtcgaggac caggagcagg tcgacaagat ccagtcggtc ctggagcgcg
      841 tgccaaagct tcagttgatg ctctacgacg aaaaaagggg cctgcgcgat tatgatcaca
      901 gccggctgca ttcgatggac gacgtcatcg aagagggccg caaggcgctc gccaacgatc
      961 catcggttcg cgcctggctg gacggcgaga tcgcggccgg caaaggcagc gatccctcaa
     1021 ttatacttta tacgtctggc acgaccggtg cttccaaggg agttctgctg accggcgagc
     1081 gttcgataaa ggcggcgtcc gataccgttg ccttcgacaa actcaccgag cacgacgtgt
     1141 cgcttgccta cctgccgctc gcctgggtcg gtgatcacta tctcaattac gcacaggggc
     1201 tggtctccgg attttgcacc gcttgtccgg aaagttccga tactgcgatg gccgatctgc
     1261 gtgagatagg gccaagcttt tattttgcac cgccgcgcac actggaagtg ctgctgacgc
     1321 gcgtgacgat ccgcatggag gatgccgggt tcctcaagcg caggatgttc cattatttcg
     1381 tcggcgtggc acgcaaatac ggcgagcgca tactcaacca cgaacacgtg ccactcaccg
     1441 gccggctcct ctaccggctg ggcgacctat tggtctatgg tccactcaag aacgtgctcg
     1501 gcctctcgcg cgtgcgcgtg gcctacactg ccggcgaagc aatcggtccc gacctctttg
     1561 cgttttatcg ctcgatcggc ctcaatctga agcagctata cgggcaaacc gaagcgttcc
     1621 tctatctcac ggcgcagcct gacggtgaaa tttactccga cacagtcggt cgaaccttcc
     1681 cgaacgtgga tatccgcatt gcggaaaacg gagaagtcct attcaagtcc ccgggcatgt
     1741 ttgcggggta cttcaaggac gatgccaaga cggccgaggt gatgacgccg gacggctatg
     1801 tgaagaccgg cgacgccggc tacttcgacg agaagacggg ccatctgaaa atcatcgacc
     1861 gggccaggga tgtcgggcgg ctcaatgacg gaacgttgtt tccgccgaaa tacatcgaga
     1921 acaagctcaa gttttatccc aatatccgcg aggcggtggc gtatggcgac aagcgcgact
     1981 ttgtcgctgt gatgctcaac atcgatcttg ctgcggtcag cagttgggcg gagcgcaaca
     2041 acgtagttta cggctcgtat caggaactcg ccggtcatcc gctggtctac gatatgctcg
     2101 aaaagcacgt ggcggaagtg aaccactcgc tggcccagga taaaatgacg gcgggcgcgc
     2161 agatcaaacg cttcctgatt ttgcacaaag agctcgatcc cgatgacggt gaaatcaccc
     2221 gcacattgaa agttcgtcga ggctttatcg aagaacgata cgcgccgctg ataaaggcgc
     2281 tttatgacgg ctcgaaagaa gccgatatct cgacggaggt gacgtttgaa gacggacgca
     2341 agggcgtcat cagtgcccgt gtgaagatcc gtgacatgaa aacagagccg gttgcggcgg
     2401 c
//
LOCUS       DASSRL010000044         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1698586, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000044 DASSRL010000000
VERSION     DASSRL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698586"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFL49_00905"
     CDS             complement(<1..962)
                     /locus_tag="VFL49_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910567.1"
                     /translation="MVEDQSSGPGKLAAANALGQLAIAHANKGNLEEAFRTLARLKDR
                     TRQDPPAIVGIVTANLAEAQAKHGDVDGAVETAMILAGENPLPLMHIVGDRVRKGNLQ
                     AAQDIIARLDDASQRYAQWGIMQAQIEHGRLIDAQVTASAIKPGHAKASALLELATYH
                     REHRGRPLALTLLEEAEGSARLTMNDWTRADVLWRIAVETAMAGDADRAMSIAKSIEQ
                     DGHRSSAIYDVAQAQAKRGDFAGAFNTASLLKQPPLTTALKVSHYDSALSNILMEMVK
                     AGKGKEARDTAAGFQDAEIRRSWLYSGIAEAYAQLGNVKAAKAAL"
     gene            complement(1313..1717)
                     /locus_tag="VFL49_00910"
     CDS             complement(1313..1717)
                     /locus_tag="VFL49_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910568.1"
                     /translation="MKSEQERQEHRRFVQALQHEHLTCTHNGCGGPMDVTDLTPHHAR
                     VKTYEALCERCHNKEQISGREQTSPAWDIASITMMAEVHLLHEQPTCPFDDTPITFIS
                     MPNPRRKARYRLLCYYCGRHTEMNWPPPEAKR"
     gene            complement(2319..3041)
                     /locus_tag="VFL49_00915"
     CDS             complement(2319..3041)
                     /locus_tag="VFL49_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7910569.1"
                     /translation="MALITGGAKGIGRGIALDLAGKHWKIAFCYRTSETDAKDTAAAI
                     RERGGEPLSVRCDVSDPAAAKQLVARVEQEWGRIDALINGAGPYHRVNLFEETVEGWL
                     QMFDGNLHPIFYLAQAAAPGMKARRSGRMINFSMANADQMISQPDVTAHYIAKAGVLI
                     LTRTLAKLLAPHGITVNAISPGFIDSGSAPPGELAAMTKRIPAGYIGTVDDTVAAVRY
                     LLSPDARYVNGANIQISGAWGV"
BASE COUNT          561 a          934 c          974 g          686 t
ORIGIN      
        1 agcgccgcct tggccgcttt gacgttcccc aactgggcat aggcctcggc gatgccactg
       61 tacagccagg agcgtctgat ctccgcatcc tgaaatccgg ccgccgtgtc tcgtgcctcc
      121 ttgccttttc cggccttcac catttccata agaatattgg acagcgcgct atcgtaatgg
      181 gagaccttca aggcagtggt gagcggcggc tgcttcagca agctcgcggt attgaatgca
      241 cctgcgaagt cgccccgttt cgcctgagcc tgcgcaacgt cgtaaatggc ggagctgcgg
      301 tgtccgtctt gttcgataga tttggcgatg gacatggcgc gatcggcgtc gcctgccatt
      361 gccgtctcga cggcgatgcg ccacaagaca tcggcgcgcg tccaatcgtt catggtcaat
      421 cgcgctgagc cttcggcttc ctctaacagc gtcaaggcca gcggccgccc ccgatgctcg
      481 cgatgatacg tcgccagctc cagcaatgca ctggccttcg catgccccgg tttgatggcc
      541 gaggctgtta cttgagcatc gatgaggcga ccgtgttcga tctgcgcctg catgatgccc
      601 cattgcgcat atcgttgaga cgcgtcatcc aggcgcgcaa tgatgtcctg cgccgcttgc
      661 agatttccct tgcgcacgcg gtctccgacg atgtgcatga gaggaagagg attttctccg
      721 gccaggatca tggcggtttc tacagcccca tcgacatcgc cgtgtttggc ttgcgcttca
      781 gccaggttcg cagtgacgat gcccactatt gcaggcgggt cctgcctcgt ccgatccttg
      841 aggcgggcaa gcgtgcggaa ggcttcttcg agattgccct tgttcgcatg cgcaatggcg
      901 agttgtccca gcgcattggc ggccgccagt ttgccaggcc ctgaagactg atcttcgacc
      961 acgatttcgg catagatccg ttctgtttca acggtgtctc cacgtgccgc atacgcttgt
     1021 gccagggcag tgaagtagtt gagcttgtgc ggaagcttgg gaaagaccct cagcgtgtct
     1081 ctggcgtccg gcgggtcgat cgcgatctgt gcgacgacaa cgggatcgag ggccgctgat
     1141 cgctccgcgc gatcgtcgat ggatgcggcg gcgcttctgg cttcgaggag aaccgcattc
     1201 ggcgattccg acgtgagcgg ctgggcagcg tgcgttgccg tggaggtcac aagagcaacg
     1261 catatgagaa cgaacgagga cgattgatga aaacgtgacc gtggcaggcc tgtcaccgtt
     1321 tggcttccgg cggcggccag ttcatttccg tgtggcggcc gcagtagtag cacagcaacc
     1381 ggtaacgcgc cttgcgacgc gggttcggca tggaaataaa cgtgatcggc gtatcgtcga
     1441 atggacaggt cggctgctcg tgaagcagat gcacttcggc catcatggtg attgatgcga
     1501 tatcccaggc gggcgacgtc tgctcgcggc ctgagatctg ctccttattg tggcaccgct
     1561 cgcacagggc ttcgtacgtt ttgacgcgcg catggtgcgg cgtcaggtcg gtcacgtcca
     1621 tggggccgcc gcatccgttg tgagtgcagg tgaggtgctc gtgttgcagc gcctgcacaa
     1681 accggcgatg ctcctgccgt tcctgctccg atttcataag ctatgctcct tataaaaaca
     1741 cactcgcatc tttttcccct ctcgccggtg ggacagtgtt gctgacctga catgccccgt
     1801 cgtttcaacc ctaaaaagtg atatctttgc gttacgcgac catgaccgaa ggggcgagga
     1861 tcaacctttt ttacccacca ccaacccacc gattgctttg caatcgttta cccggggtga
     1921 gccgggaaga aaaactggaa ctaggtatgc tcctaccgag ctcgcttgat gtccctttgc
     1981 aggcaggtga atggtcgact tctgtgacct gcgaggggtt catggcctta tcacagtggt
     2041 cgctcggcga tggcgagacg ccgtgttcga aggtccgttc ggacggaggc cggaacggac
     2101 caagttggcg gagccaaact gtgacagggc catgaggttt cactcctcgc cggaacagag
     2161 gcaccatcca cctgccattt tttcctgtgc gcaagttccg cccggcgaag cgctcgcgga
     2221 tcgatccgag aggtcgagga gcgagacgca aaggtatcgc gaatgcgata tggctggcgc
     2281 ggtgcgcgag caaccgaggt tggcacattg gcatgccgct acacccccca cgcgccgctg
     2341 atttgaatgt tggcgccgtt cacatagcga gcgtcagggc tcaacagata gcgcacggcg
     2401 gcgacggtgt cgtcgaccgt gccgatgtag ccggcaggaa tgcgtttggt catggcggca
     2461 agttcgccgg gcggcgcgct gcccgaatcg atgaatcccg gcgagatggc gttgaccgta
     2521 atgccgtggg gcgccagcaa tttggccagg gttcgcgtga ggatcagcac gccggctttg
     2581 gcgatgtaat gagcggtcac atccggctgg gagatcatct gatcggcgtt ggccatgctg
     2641 aaattaatca tccggccgga ccgccgcgct ttcatgcccg gcgccgcagc ctgcgccaga
     2701 tagaagatcg ggtgcaggtt cccgtcgaac atctgcagcc agccttccac cgtttcctcg
     2761 aacaagttca cccggtgata gggcccggca ccgttgatca atgcatcgat gcggccccat
     2821 tcctgttcga cacgggcgac caattgtttg gccgcggccg ggtccgagac gtcacagcga
     2881 acggacaagg gctcgccgcc gcgttcgcgt atggccgccg cggtatcctt ggcgtccgtt
     2941 tcgctggtgc gatagcagaa cgcgattttc cagtgtttgc cggcgaggtc gagcgcaatc
     3001 ccgcgtccga tgcccttcgc tcctccggtg atcaacgcca cattcgtgct catgacgtcc
     3061 tctcaagtgc gggaacgcag ccgtttggca agcagttgca acgcgcctga cgttctcatg
     3121 gagaacgatt gttcctgttt tggtttcctg gcgtc
//
LOCUS       DASSRL010000045         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_806395, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000045 DASSRL010000000
VERSION     DASSRL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806395"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..576
                     /locus_tag="VFL49_00920"
     CDS             112..576
                     /locus_tag="VFL49_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET7910570.1"
                     /translation="MKVRAGELGGLFLFLALVLGGGLVIGFVTAPGAWYVTLNKPPFN
                     PPSWVFAPVWTALYIMIATAGWRAWREGRSSAPMKLWWVQLVLNFAWSPIFFSAHRID
                     IALGVILLLLTTIITFIVISWQRDKIASLLFAPYAAWVAFAAVLNGAIWLLN"
     gene            589..1098
                     /locus_tag="VFL49_00925"
     CDS             589..1098
                     /locus_tag="VFL49_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910571.1"
                     /translation="MQCRIVIVNDKMQHGYRYVLSAQIGRGFDPEFQPDLTPRQMLEL
                     GVFCGKYLTDCREEYPASWFARAKLAPSRRDCSLNYFGVDASQPLSEWRRKGWIHPDD
                     PRGWFQWYCRYYMGRRLSEEDVRQVGRWKAMQRHVAQIKKHCEPGDLTCRRRQRQALL
                     HWAYDSRRF"
     gene            1380..1526
                     /locus_tag="VFL49_00930"
                     /pseudo
     CDS             1380..1526
                     /locus_tag="VFL49_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1643..2128)
                     /locus_tag="VFL49_00935"
                     /pseudo
     CDS             complement(1643..2128)
                     /locus_tag="VFL49_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
     gene            2179..2866
                     /locus_tag="VFL49_00940"
                     /pseudo
     CDS             2179..2866
                     /locus_tag="VFL49_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544848.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          535 a          908 c          880 g          543 t
ORIGIN      
        1 ctggccttct tatcgacagg cacgctcata tgaggacgcg atcatcgtcg ctgatccgag
       61 gcaaaatgaa tgatgacgtc ctctgccgaa acgaggctcg tgatgattcc gatgaaagtg
      121 cgtgcaggcg aactcggcgg cctattcttg tttctcgcgc ttgtactggg tggcggcctt
      181 gtcatcggtt tcgtgacagc ccccggtgca tggtatgtaa cgctcaacaa gccgcccttc
      241 aatccgccga gttgggtctt tgccccagtc tggactgcgc tctacatcat gatcgcgacc
      301 gccgggtggc gggcttggcg agagggccgc agcagcgcgc caatgaagct atggtgggta
      361 cagctggttc taaatttcgc ttggtcaccc atttttttct cagcgcatcg catcgatata
      421 gccttaggcg tgatcctgct gctcctgacc acgatcatca ccttcatcgt gattagctgg
      481 cagcgggata aaatcgcttc gctcctgttt gcgccctatg ccgcatgggt ggcgttcgcc
      541 gcggttctga atggcgcgat ctggctcttg aactgatcgg gtgggcgaat gcaatgccgc
      601 atcgttatcg tcaatgacaa gatgcagcat ggctatcgct atgtgttatc ggcgcagatc
      661 ggccgcggtt tcgatccaga attccagccc gatttgacgc ccagacagat gctcgaactc
      721 ggcgtgttct gcggcaaata cctgaccgat tgccgggagg agtatcccgc aagctggttt
      781 gcgcgcgcca agctcgcacc atcgaggcgc gactgctcgc tcaattattt cggcgtcgat
      841 gcgagccagc ctttgtcgga atggcgaaga aagggctgga tacacccaga cgatccgcgc
      901 ggctggttcc agtggtactg ccgatactat atgggccgcc gcctgtccga ggaggacgtg
      961 cgccaggtcg gccgctggaa ggcgatgcaa agacatgtcg cgcaaatcaa aaaacattgc
     1021 gagccgggcg acctaacgtg ccggcggcgc cagcgccaag cgctactgca ttgggcctac
     1081 gacagtagga gattctagct ggaggccgcc tcgtcacgta ccgcttactt gccgtcgcaa
     1141 ggattagagc atcgcctgaa acactccgcg gcacccgggg cgcgagccca caagaaatgg
     1201 ccgacgcgcg gggtgaaaga gcggctgcta tcgtggcggc ctgtgcgacc gccgcgtcaa
     1261 gtgtcggtgc cggataaggc ctgagaacaa ccaggagatc agaacgccat taggtcctct
     1321 gcagtcgttg aaacgctgca aacggatatg cggctttccg agcatttgag gactatcccg
     1381 agcgcgagga caagaactgg ctgaagcata cggcggtctg ggtcgatgcc gagacgggca
     1441 agacccgcat cgactatcgg ccggtcaacc tgcagccgat gagcaacgac gttcagtcct
     1501 tcccgcccaa ggcgcgggtg tactgaggga cggccctgat gcggtaatcg agtgatgtcg
     1561 gtgttggctc taagcggacc atgttctcgc gacgtcgccg ccatccgtcc tcacaacacc
     1621 gacttgggtt ttgggtcgcc ggttaccagc tttggagcgc gcgaagctgc ggcgtcttct
     1681 tgctcatatg cccgctcatg cggccgagaa gcaggccgag ctgcccgatg ccttcgaaga
     1741 gataaggtcc cttgttcagc atcacgcatt ccgcacgagc cgccattgcc gcatcggtca
     1801 tctcgccgcg cgtgggcacg ccggtcttca ggtagctttc gagcacctgt gtggcccaga
     1861 tcaccggaac gtgcgccgcc tcgcatagcc agaggatttg ctcctgcatc tcagccagac
     1921 ggacgaagcc gatctccacg gccagatcgc cgcgcgcgat catgatcgcg gagggctgcc
     1981 gccccgccgc ccggaccagc atatccggca ggttcctgac cgcgcgcgag gtcacgatct
     2041 tgagaaccag ccccaacaga tgccagtctt cgggtcgctc ccgggccagc gcctcctgca
     2101 attccgcgac gtcctcatgg ctctgaaggt gcggccaccg ccgccgatct gatgctgcag
     2161 ctggcggcgg cacgggccca ccggccgaag aagctggtcg gcgagcaccg ggactggctg
     2221 ctgcagcgct gccgggcggg cgctctcacg ctgcgcggcc tggtcgccga actggccgaa
     2281 cgcggcctgc gcgtggacta tcgcgtggtc tgggcgttcg tccacgagga gaagctgagt
     2341 tacaaaaaaa gaccctgatc gccagcgagc gcgatcggcc cgacgtggcg cgccggcgcc
     2401 agcagtggat ggcctatcag gatcgcatcg atccggcccg cctggtcttc atcgacgaga
     2461 cctggaccaa gaccaacatg gcgccgctgc ggggctgggg tccgttgggc cagcgcctgc
     2521 ccgccaaggt gccgcacggc cgacggacga ccatgacctt cctcgccgcc ctgtgccacg
     2581 atcgtgtcga ggctccctgg ctcattgatg gcccaatcaa tggcgagagc ttccgcctct
     2641 acgtcgacaa ggtgctcatc ccgaccctgc agcccggcga catcgccatc atggacaatc
     2701 tcggctcgca caagggccgc gccgtccgat gcgccctcag agcggccagg gcaaagctct
     2761 tcttcctgcc caagtactca ccggatctga acccgatcga gatgctcttc tcaaagctca
     2821 agcacggctt gcgcaaggcc gcagcgcgta cccacgatgc cgtctg
//
LOCUS       DASSRL010000046         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1576102, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000046 DASSRL010000000
VERSION     DASSRL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576102"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFL49_00945"
     CDS             complement(<1..816)
                     /locus_tag="VFL49_00945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7910572.1"
                     /translation="MPMNVATLAGARSIRPEPPVTSADLAAIKARQHAAWSSGDYAVV
                     GTTLQIVGEELCEALDLRSGRKVLDVAAGNGMMSLAAARRWCDVISTDYVANLLERGR
                     ARALAEGLPIEFRVADAEALPFDDNAFDTVVSTFGVMFTPNQDRAASELLRVCMSGGQ
                     IGLANWTPDGFIGHLFKTLGKYISPPAAAKSPLLWGTRARLTELFGPAAVWIKTESRH
                     FNFRYRSAEQFVDVFRTFYGPTLKAFAALDIRKQNALEKDLYDLIARMNKADDG"
     gene            953..>2467
                     /locus_tag="VFL49_00950"
     CDS             953..>2467
                     /locus_tag="VFL49_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HET7910573.1"
                     /translation="MRFLFGDHTLDADRRELRRGSEPIAVEPQVFDLLVYLVENRDRV
                     VSKDDLIASVWGGRVVSDSTLTSRINAARKAVGDSGENQKLIRTIARKGLRFIGDVSV
                     SPLGVDAPDMLTRSHADSQEASRPALPLPERPAIAVLPFVNMSGDPEQEYFSDGISED
                     IISALSKLRWFFVIARNSSFTFKGKSVHMKQIAEELGVGYVVEGSVRKSGERVRITAQ
                     LNDVATGTHIWAERYDRDIADVFAVQDEITEAIVAAIEPQLYAAENFRAQRKPPDSLD
                     AWDLVMRGLSHYWRLTRQDNMVAQAMLEKATAIDPNYGQALGVLAAAHTFSAHMGWSE
                     MAKAVPIAERAAIAAIRADSEDPWAHYALGNVCLFTRRFDDSLAEFETALRLNPNFSL
                     AQCYYGLTLCYCGRWQDGDLAARRALRLSPRDPFSALYYGIASYSQYSGHNYDEAMRL
                     AREAIRERNDFVGGHRVLTASAGMAGRNEVAAAALQELRRVQPNISLSWISKEMP"
BASE COUNT          471 a          741 c          781 g          474 t
ORIGIN      
        1 gccgtcgtcc gccttgttca tgcgggcgat gagatcatag aggtccttct ccagggcatt
       61 ctgcttcctg atatcaagag ctgcgaatgc tttgagggtc gggccataga aggtcctgaa
      121 gacatcgacg aattgctctg ccgaccgata acggaaattg aaatgacggg attccgtctt
      181 gatccagacc gcggccggac cgaacaattc ggtcagacgc gcgcgtgtgc cccagagcag
      241 cggagatttt gccgccgccg gcggtgagat gtacttgccg agcgtcttga agaggtggcc
      301 gataaagccg tccggcgtcc agttcgcaag tccgatctgg ccgcccgaca tgcaaacgcg
      361 caggagctcc gacgcggcac ggtcctgatt gggcgtgaac atgacgccga atgtcgagac
      421 gaccgtgtcg aatgcgttat cgtcgaacgg caatgcttcg gcgtccgcaa ccctgaattc
      481 gatcggcaag ccctcagcca gcgcgcgcgc acggccgcgc tccagcagat ttgccacata
      541 gtcggtggag atgacgtcgc accagcgtcg ggcagcggcg aggctcatca ttccgttgcc
      601 ggcggccacg tcgagcacct tccggccgga gcgaaggtca agcgcttcgc aaagctcctc
      661 cccgacaatc tgcaaggttg tgcctacgac cgcgtagtcg cctgaggacc aggctgcatg
      721 ctgccgggcc ttgattgcgg caagatcggc tgatgttacg ggtggctcag gccggattga
      781 ccttgcgcct gcaagggttg caacgttcat cggcatctcc tgtccaattt ccaacatgat
      841 tgggaggtga gtaccggcaa atggcgcgaa aggctttgcc cgggccttga tttgttcttg
      901 aggcgggctt gattatttcc cgagacccgg cttgcactgg tggggggcgc caatgcggtt
      961 tctctttggc gatcatacac ttgacgccga ccgccgcgag ctgcgccgcg gcagcgagcc
     1021 cattgccgta gagccgcagg tattcgattt gctggtttac ctggtcgaga atcgcgaccg
     1081 ggtggtcagc aaagacgacc tgattgcctc cgtttggggt gggcgcgtcg tttcagattc
     1141 aacgctgacc agccgcatca atgcggcacg caaagcggtg ggcgacagcg gcgaaaacca
     1201 gaagctgatc cgaacgatcg cgcgcaaagg acttcgcttc atcggcgacg taagcgtgag
     1261 tcccctgggc gtggacgcgc ccgacatgct gacccgttct cacgcggaca gccaggaggc
     1321 atcccgtcca gctttgccct tgcccgaacg gccggcgatc gccgtgctcc cgtttgtgaa
     1381 catgagcggc gatcccgaac aggaatattt ttcagacggt atcagcgaag acatcatctc
     1441 cgccttgtcg aagctgcgct ggtttttcgt gatcgcgcgc aactcttcgt tcacgttcaa
     1501 gggtaaatcc gtccacatga agcagatcgc cgaggagctc ggcgtcggct atgtcgtcga
     1561 aggcagcgta cggaaaagcg gcgagcgcgt gcgtatcacc gcgcagctca acgacgttgc
     1621 gaccgggacg cacatctggg cggagcggta cgaccgtgat atcgccgacg tcttcgcggt
     1681 acaggacgaa atcacggaag cgatcgtcgc cgcgatcgaa ccgcagcttt atgccgcgga
     1741 aaatttccgc gcgcagcgca agccgccgga cagcctggac gcatgggatc tggtgatgcg
     1801 gggcttatcg cactactggc ggctgacgcg gcaggacaac atggtcgcgc aggccatgct
     1861 ggaaaaggcg actgcgatcg atccaaatta cgggcaggcc ctcggtgtgc ttgccgcggc
     1921 ccatactttc agcgcgcaca tgggctggtc ggagatggca aaggcggtac cgattgccga
     1981 acgagcggcg atagcggcga tccgcgccga cagcgaggat ccgtgggcgc attatgcgct
     2041 gggcaatgtc tgcctcttta cgcggcgctt cgacgattca ctcgccgaat tcgaaaccgc
     2101 gctgcgcctc aatccgaatt tttcgctggc ccagtgctat tacggactga cgctctgcta
     2161 ctgcggccga tggcaggacg gcgatctggc ggcgcgccgc gcgctgcggc tgagcccgcg
     2221 cgaccccttc tcggcgcttt attacggtat tgcttcgtac tcgcagtaca gcggccacaa
     2281 ttacgatgaa gcgatgcggc tcgcccgcga agccatccgt gaacgcaacg attttgtcgg
     2341 cggtcacagg gtgctcaccg catccgccgg catggccgga cgtaacgagg tcgcagcggc
     2401 ggcgctgcag gagctccgcc gcgtccagcc caatatttcg ttgagctgga tttcgaagga
     2461 gatgccc
//
LOCUS       DASSRL010000047         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1226191, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000047 DASSRL010000000
VERSION     DASSRL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226191"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1185
                     /locus_tag="VFL49_00955"
     CDS             7..1185
                     /locus_tag="VFL49_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7910574.1"
                     /translation="MLKDTDFTWEDPLGLEHELTEDERMVRDTARGFAQDYLMPKVTE
                     AYREEKYDPNNLPEMGKLGLLGPTIPEEYGGAGLGYVCYGLITRELERVDSGYRSSMS
                     VQSSLVMYPIYAYGSEAQRRKYLPKLATGEMIGCFGLTEPDHGSDPGSMVTRAEKVSG
                     GYRLNGAKTWISNSPVAHVAVVWAKLDNVIRGFLVERGARGFSTPKIAGKLSLRASIT
                     GEIVLDDCVVPEDNLLPNVKGLAGPFGCLNNARYGISWGAMGAAEFCWHRARQYVLER
                     KQFGRPLAANQLIQKKLADMQTEIALGLAGALRLGRMLEAGKAAPASISLMKRNNCGK
                     ALEIARLARDMHGGNGISEEYHVMRVLSNLETVNTYEGTHDIHALILGRAQTGIQAFF
                     "
     gene            1408..>2566
                     /locus_tag="VFL49_00960"
     CDS             1408..>2566
                     /locus_tag="VFL49_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET7910575.1"
                     /translation="MRSNTVEVDSSERPSRLRFAPTFNVAVPFIDRHVSEGRGPKTAI
                     LTKDSVTTYADLAAGVNRCASGLVRLGLRRGDRILMVVKDCPAFFFLFWGAIKAGIVP
                     VPLNTLLRSSTYSFMIDDSNAALLVYSPEFSDEVAAGVAGAKCTPRYVLRTEGEGYSL
                     ASLLAESAPDFEAVPAAAEDDCFWLYSSGSTGQPKAAIHRHRDMVVTSQRFAIETLGI
                     RKTDVLYSEAKLFFAYGLGNNLTFPLWVGATAVLNDSRPGPATSFSLITRFQPTFYFG
                     VPTLYAAYLQHFAHEKPDLSSIRFCVSAGEALPAEILRRWHEETGLWILDGIGSTEAL
                     HIFISNSPNDIRPGSSGRVVSGYSARIFDENGADVADGESGQLFISGDSTAR"
BASE COUNT          493 a          747 c          779 g          547 t
ORIGIN      
        1 cccaagatgc tcaaggacac cgacttcacc tgggaggacc cgctcggcct cgagcatgag
       61 ctgaccgaag acgagcgcat ggtgcgcgac accgcgcgcg gcttcgcgca ggactacctg
      121 atgccgaagg tgacggaggc gtaccgcgaa gagaaatacg atccgaacaa cctgcccgag
      181 atgggcaagc tcggcctgct cggcccgacc attccggaag agtacggcgg ggcggggctc
      241 ggctatgtct gctacggcct gatcacgcgc gagctcgagc gggtggactc cggctaccgc
      301 tcctcgatgt cggtgcagtc gtcgctggtg atgtatccga tctatgccta cggcagcgag
      361 gcgcagcggc gcaaatatct gcccaagctg gcaaccggcg agatgatcgg ctgcttcggc
      421 ctgaccgagc ccgatcacgg ctccgacccg ggctcgatgg tgacgcgggc ggaaaaggtg
      481 tccggcggct acaggctcaa cggcgccaag acctggatct cgaattcgcc ggtcgcgcac
      541 gtggccgtgg tgtgggcgaa gctcgacaac gtcatccgcg gcttcctggt cgagcgcggc
      601 gccaggggat tttccacgcc gaagatcgcg ggcaagctgt cgctgcgcgc ctcgatcacc
      661 ggcgagatcg tgctggacga ctgcgtggtg ccggaagaca atttgctgcc caacgtgaag
      721 ggtctcgccg gtccgttcgg ctgcctcaac aatgcgcgct acggcatctc ctggggggcg
      781 atgggcgccg ccgagttctg ctggcaccgc gcgcggcaat acgtgctcga gcgcaagcag
      841 ttcggccggc cgctcgccgc caaccagctc atccagaaga agctcgccga catgcagacc
      901 gagatcgcgc tcggcctcgc cggcgcgctg cggctcggcc gcatgctgga ggcgggcaag
      961 gcggcgccgg cctcgatctc gctgatgaag cgcaacaatt gcggcaaggc gctggagatt
     1021 gcgcggcttg cccgcgacat gcacggcggc aacggcatct ccgaggaata ccacgtgatg
     1081 cgcgtgcttt cgaacctcga gacggtgaac acctatgagg gcacccatga catccacgcg
     1141 ctcatcctcg gccgcgcgca gaccggcatc caggcgtttt tctgaaaacc tgttcctcgc
     1201 gtatcagaac attcgccaga ctgatcgact tgatctgcgc atcgaagcgg cgcctttgcg
     1261 tagcaacacg aactgtcgct gcagatggat tggcgttttc cggttgaccg aagtcaagga
     1321 taagtcggtt tgcggcagct atggtttcgc caattgaatg gatggccaat tgattgttgt
     1381 cgtcgaactt atacgcaggt taagacgatg cgcagtaaca cggtcgaagt cgactcttcc
     1441 gagcggccat cgcggttgcg cttcgcgccc acttttaatg tcgctgttcc attcattgac
     1501 cgacatgttt cggaagggcg cggtcccaag acagcaatac tgacaaaaga tagcgtcacg
     1561 acgtacgctg atctggcagc cggcgttaat cgctgtgcga gcggccttgt gcgccttggt
     1621 ctccgacgcg gagaccgcat attgatggtt gtaaaggact gccccgcgtt tttctttctt
     1681 ttttggggtg cgatcaaagc cggaattgtg ccggtgccgc tcaatactct tttgcgttcg
     1741 agcacttact ctttcatgat tgatgactca aacgcagcac tgcttgtcta ttcacctgaa
     1801 ttctccgacg aagtggctgc cggtgttgcg ggcgccaagt gcacaccgcg ttacgtcctg
     1861 cgcaccgagg gtgagggcta ttcccttgcc agtctgctcg ctgaaagcgc gcccgatttt
     1921 gaagctgtgc cggcagcggc tgaagacgat tgcttctggc tctactcttc cggatccacc
     1981 ggacaaccga aggcggcaat ccaccgccat cgcgacatgg tggtcaccag ccagcgattt
     2041 gccatcgaaa cattgggaat tcgcaagacc gacgttctct attcggaagc gaaattattt
     2101 ttcgcatatg ggttgggaaa caatctcacg tttccccttt gggtaggcgc cacggcagtt
     2161 ttaaatgact ctcgccctgg gccggcaacg agtttttcgt tgattacgcg ctttcagcca
     2221 acattctatt ttggcgttcc aacgctttat gcggcctact tacagcattt tgcacatgag
     2281 aaaccggatc tttcttcgat tcgattctgc gtatcggcgg gggaggctct tcctgccgaa
     2341 attcttcgcc gttggcacga agaaacaggg ctttggattt tggatgggat cggttcgacg
     2401 gaagcgctgc atatattcat ttcaaattca ccgaacgata tcaggccggg gtcaagtggc
     2461 cgggttgtct cggggtattc ggctcgtatt tttgatgaaa acggggccga cgttgccgat
     2521 ggagagagcg gccaactgtt catttccgga gattcgactg cgcgct
//
LOCUS       DASSRL010000048         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_613926, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000048 DASSRL010000000
VERSION     DASSRL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613926"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFL49_00965"
     CDS             <1..1033
                     /locus_tag="VFL49_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7910576.1"
                     /translation="KLKLPPGFNIEVYASGIANTRSLRVGDKGTVFVGTRLGNKVTAI
                     VKKDGKTELKTVAQGLYRPNGLAYHKGTLYIAELSQISKIENVENVLDNPPKPTVIYT
                     DLPKDEAHGWKFIGVGPDNKLYVPVGQPGNNVMNDKNHAQIRRINLDGTGREDVALGV
                     RNTVGFDWNPVNKQLYFSDNGRDWLSEDVPQDELNRVTKIGQHFGSPHCYQGNFLDPE
                     FGWGHTCSEFVPPIMLTGPHSAGLGLRFYTGNMFPKKYKNAIFLARHGSWNRSKKFGG
                     DVLAIYLNKDGTVKSSEPFLTGFLENNNYVGRPVDVEFMADGSMLVSDDWNGAIYRIT
                     YGKPRVAGR"
     gene            1091..1705
                     /locus_tag="VFL49_00970"
     CDS             1091..1705
                     /locus_tag="VFL49_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7910577.1"
                     /translation="MKTINSAHRAAAAALFFCLAFYSARGETIAERAAPCLACHGESG
                     QSQTEGVPSLGGQPAPYLLIQLYMFREQQRAAPEKKDDPMIRVMTELTKGFSDDDLRN
                     FSAFIAKLPAPKPPANEGDAARMQAARALITQNRCNSCHGLDMAGRDNVPHIADQRED
                     YLVKTLREYKTNARRGYDGSMAEVLAPVSDQQIVDLAYYIARFR"
     gene            complement(2004..2897)
                     /locus_tag="VFL49_00975"
     CDS             complement(2004..2897)
                     /locus_tag="VFL49_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7910578.1"
                     /translation="MDWDKLKVFHAAAEAGSFTHAGERLGLSQSAVSRQVSALEAELS
                     VSLFHRHARGLILTEQGELLYRTAHEVFMKLEAARTKLTDSRERPNGELKVSTTPGIG
                     VHWLTPRLGEFLDLYPDIHITLITTDEELDLAMREADVAIRLRQPTQPDLIQRKLFSV
                     HSHAYASPDYLKRFGTPRTHDDLDKHRIILLGGGAVPAHLQNRRWLLEIGRESKGPRA
                     PHLTVTNVLGVLRACQRGLGVAMLPDYLVEENGGLVQLFGEADTLAMDAYFVYPEELK
                     SVARIQVFRDFLVANAQRWHF"
     gene            complement(2922..3887)
                     /gene="trxB"
                     /locus_tag="VFL49_00980"
     CDS             complement(2922..3887)
                     /gene="trxB"
                     /locus_tag="VFL49_00980"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7910579.1"
                     /translation="MTKTTHAKVVIIGSGPAGYTAAVYAARAMLEPILIQGVQPGGQL
                     TITTDVENYPGFAEVIQGPWLMEQMQAQAEHVGTRIVTDHVNKVELSQRPFKLICDSG
                     DVYLADALIIATGAQARWLDLPSEQKFKGYGVSACATCDGFFYRNKEVIVVGGGNTAV
                     EEALFLTNFASKVTVVHRRDAFRAEKILQDRLFKHPKIEVIWDSVLEDVTGAENPLKV
                     SGVKLKNVKTGAITGHKADGVFIAIGHQPASELFTGQVQMKPSGYIVTAGHSTATSVP
                     GVFAAGDVTDDIYRQAVTAAGQGCMAALEAEKFLAAQEHRRAAAE"
     gene            4129..>4399
                     /locus_tag="VFL49_00985"
     CDS             4129..>4399
                     /locus_tag="VFL49_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7910580.1"
                     /translation="MPARLDAIDRKILKELQEDGRITNVELASRVGISAPPCLRRVRA
                     LEEAGYIKGYRALLDEKKLGYEVTVFAMVHLSSQAEADLVAFESFV"
BASE COUNT          843 a         1348 c         1365 g          843 t
ORIGIN      
        1 caagctcaag cttcctcccg gattcaacat cgaggtctat gccagcggca ttgccaatac
       61 acgctcgctg cgcgttggcg acaaaggcac cgtctttgtc ggtactcgcc tcggcaacaa
      121 ggtcaccgcg atcgtcaaga aagacggaaa gaccgagctc aagaccgtgg cgcagggtct
      181 ctatcggccc aatggcctcg cctatcataa aggcacgctc tacatcgcgg agctgtcgca
      241 gatttccaag atcgagaatg tcgagaacgt cctcgacaac ccgccgaagc cgacggtcat
      301 ctacaccgac ttgccgaaag acgaggcgca cgggtggaag ttcatcggcg tgggtcccga
      361 taacaagctt tacgttccgg tcggacagcc cggcaacaac gtcatgaatg acaaaaatca
      421 cgcgcaaatc cgtcgcatca atcttgacgg caccgggagg gaggatgtgg cgctcggcgt
      481 gcgcaacacc gtcggcttcg actggaatcc cgtaaacaaa cagctttact tctcggataa
      541 cggccgcgac tggctgtccg aggacgtgcc gcaggatgag ctcaaccgcg tgaccaagat
      601 cggccaacac tttggctcgc cgcattgcta tcagggcaat ttcctcgatc cggaattcgg
      661 ctggggccac acatgcagtg agttcgtgcc gccgatcatg cttaccgggc cgcattcggc
      721 cgggctcggg ttgcgcttct acaccggcaa catgtttccg aagaagtaca agaacgccat
      781 cttcctggcc cgccatggat cgtggaatcg ctcgaagaag ttcggtggcg atgttctcgc
      841 catctacctg aacaaggatg gcacggtgaa atcgtccgag ccgttcctta ccggcttcct
      901 tgagaacaac aactacgtcg gccgcccggt cgatgtggag ttcatggcgg acggttcaat
      961 gctcgtgtcc gacgactgga acggcgcgat ttatcgcata acttacggca agccgcgcgt
     1021 tgccggccgg taagttggac cagcgccggg agcgtcctgc tcccggccat attcctctca
     1081 gccagtcggt atgaagacga tcaattccgc gcatcgcgcc gcggctgcgg cgttgttttt
     1141 ctgcctggcc ttctattcgg cacgcggtga gacgattgcc gagcgtgcgg cgccgtgtct
     1201 tgcctgtcac ggcgagagcg ggcagtcgca gacggagggc gtcccttcac ttggcgggca
     1261 gcccgcgcct tatcttttga tccagctcta catgtttcgc gagcagcaac gtgcggcgcc
     1321 ggaaaaaaaa gatgacccga tgattcgggt gatgaccgag ctgacgaagg gcttctccga
     1381 cgacgacctt cgcaacttct cggcctttat cgcgaagctc ccggcgccga agccgccggc
     1441 gaatgagggc gatgcggcgc gcatgcaggc ggcgcgcgcc ttgatcacgc agaaccgttg
     1501 caacagctgc catgggctgg acatggccgg ccgtgacaac gttccgcaca tcgccgacca
     1561 gcgcgaagac tacctcgtga agacgctgcg cgagtacaag accaacgcgc ggcgcggtta
     1621 cgatggttca atggccgaag tgctggcgcc ggtcagcgat cagcagattg tcgatcttgc
     1681 atattacatt gcgcgttttc gctgagtgaa aacggcagtg cacgaaaaaa accgggcccc
     1741 gaaaggggcc cggcttcagt gtttgccggc gtgggggccg gcaacatctt ccagagggga
     1801 acaagctgca caccggtgat tctgggagga tacgcatccg gcgcaacagc gagtgcgcag
     1861 tatgcgaatg tcggagttca gcagcaacgc cgatggccgc atgccagcca tgcgcaatcg
     1921 caaatcgttg acgcctgaat aaacggcggc agaactcgcg ctttccgcgc gccgaaagca
     1981 cagtgcggtt gctgaaagaa aaactaaaag tgccagcgtt gcgcgtttgc gacaaggaag
     2041 tcgcggaaca cctggatacg cgcaaccgat ttgagttcct ccggataaac gaaataggca
     2101 tccatcgcga gcgtgtcggc ctcgccgaaa agctgcacca ggccgccgtt ttcttccacc
     2161 aggtagtccg gcagcatcgc gaccccgagg ccccgctgac acgcacgcag tacgcctagc
     2221 acgttggtga cggtcagatg cggtgcgcgc ggacctttgc tctcacggcc aatctcgagc
     2281 agccaccgcc gattctgcag atgcgccggt acggcgccgc cgccgagaag gatgatgcgg
     2341 tgcttgtcga gatcatcgtg ggtgcgcggc gtgccgaaac gcttgagata gtcaggcgac
     2401 gcataggcgt gtgaatgcac cgaaaacagc ttgcgctgga tcaggtccgg ttgcgttggc
     2461 tggcgcaggc ggatggcgac atcggcctcg cgcatcgcca gatcgagctc ttcgtcggtc
     2521 gtgatcagcg tgatgtggat gtccggataa aggtcgagga attcgccgag ccggggcgtg
     2581 agccaatgca cgccgatgcc tggcgtggtc gacaccttga gctcgccgtt cggccgttcg
     2641 cggctgtcgg tgagcttggt gcgcgccgcc tcgagcttca tgaagacttc gtgcgccgtg
     2701 cggtaaagca gctcgccttg ctcggtgagg atcagtccgc gcgcatggcg gtgaaagagt
     2761 gataccgaaa gttcggcttc gagcgcggag acctgccgcg acacggctga ctgagagagg
     2821 ccaagccgtt cgccggcatg ggtgaacgag ccagcttccg ccgcggcgtg aaacaccttc
     2881 agcttgtccc aatccatcgt ggtgttgggt gcgcccatga tctactccgc cgccgcgcgg
     2941 cgatgctctt gcgcggccag gaatttctcc gcttcgagcg cggccatgca gccctgcccg
     3001 gcagcggtca ccgcctggcg atagatatcg tcggtcacgt cgccggccgc gaatacgccc
     3061 ggcaccgatg tggccgtgga gtgcccggcc gtcacgatgt agccggaggg cttcatctgc
     3121 acctggccgg taaacagctc cgacgccggc tggtggccga tcgcgatgaa cacgccgtcc
     3181 gccttatgcc cggtgatcgc acctgtcttc acgttcttga gcttcacgcc gctcaccttg
     3241 agcgggttct cggcgccggt gacgtcctcc agcacgctgt cccagatcac ttcgatcttc
     3301 gggtgcttga acaggcggtc ctgcagaatt ttttcggcgc ggaacgcgtc tcgccggtgc
     3361 acgaccgtga ccttggacgc aaaattggtg agaaacagcg cctcttcgac cgcggtgttg
     3421 ccgccgccga ccacgatgac ttccttgttg cggtagaaaa agccgtcgca ggtggcacag
     3481 gccgacaccc cgtagccctt gaatttctgc tccgaaggca gatcgagcca gcgcgcctgc
     3541 gcgccggtcg cgatgataag cgcgtcggcg aggtagacgt ctccggaatc gcagatcagc
     3601 ttgaagggcc tttgcgacaa ttccaccttg ttcacatggt cggtgacgat gcgggtaccg
     3661 acgtgctcgg cctgcgcctg catctgctcc atcagccacg gaccctggat cacctcggcg
     3721 aagcctgggt agttctcgac atcggtcgtg atcgtgagct ggccaccggg ctgcacgccc
     3781 tggatcagga tcggctccag catcgcgcgc gcggcataaa cggcagccgt gtacccggcc
     3841 ggtcccgagc cgataatcac gactttggcg tgcgtcgtct ttgtcatgct tgtttccgcc
     3901 cccctggcgg gcgtcgcgga cgcgctgggc ggccgcaggc aggccggaca gtcaccggct
     3961 cttttgtgct ctgcccgatg tatggcttcc ggctgagcta tgcaagatac gcaactcgcc
     4021 gcacccacag gggatgccag gtggagcacg caatattgtt tcgcgaaata atatttcgcg
     4081 gtatgaatgt ttgtggccgc ctctggcccg tctctgtgga gtctttttgt gcccgcccga
     4141 ctggacgcca ttgaccgcaa gatccttaag gaactgcagg aggacggacg catcaccaac
     4201 gtggagctgg caagccgcgt tggcatttca gcgccaccct gcctgcgccg cgtccgcgcg
     4261 ctggaggagg ccggctatat caagggctac cgcgcactgc tcgatgaaaa gaagctcggc
     4321 tacgaggtga ctgtctttgc gatggtgcat ttgtcgagcc aggccgaggc cgaccttgtc
     4381 gctttcgaga gtttcgtgc
//
LOCUS       DASSRL010000049         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_596418, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000049 DASSRL010000000
VERSION     DASSRL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596418"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFL49_00990"
     CDS             complement(<1..1051)
                     /locus_tag="VFL49_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7910581.1"
                     /translation="MSVSSPFIHRPIATSLLGIAVMLGGFLGYWWLPVSALPQVDFPT
                     IQVTTQLPGANPDTIASLVTAPLERQFGQIPALQTMTSSSSFGISQVTLQFDLNRDID
                     AAAQDVQSAINAAASTLPRNLPYPPLYSKVNPADAPIITLALTSKTIDLRTLSDMADT
                     IMAPRLSEVTGVGRVSVQGGIRPAIRIQADLSRLAAYGIALDDVRTAVVGANVAGPKG
                     ALDGAHQSYTIAANDQITAASTYRDIVIAFRNGAPVKISDVADVVDGLENTKVGAWYR
                     GQPAIVIDVQRQPGANVIETVQRLRTEFPRLRRAMPAGADLTVVNDRTTTIRASIQDV
                     QFTLVLSIALVVLVVL"
     gene            complement(1048..2325)
                     /locus_tag="VFL49_00995"
     CDS             complement(1048..2325)
                     /locus_tag="VFL49_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7910582.1"
                     /translation="MHGEFPKQILAKARSGTRRTFAIALILITVAAALYYVFSESAPQ
                     QRRGGRFTPNEGPVPVLVATATRADVPVFLDAVGTIKALNTVTVRPQVDGKLLSVNFK
                     EGDDVKKGDVLAKIDPVIYRAQLDQAIAKKAQDEAQLANARIDLERYERLAATNAINK
                     QQADTQRALVAQNTALVQADQAAIENAQAMLGYTTITAPIDGRTGIRMVDEGNYVRAA
                     DTNSSIVVITQLQPISVLFNLPQQDLSQVNTAFDKSPLNVEAQRSDNDAVIDRGVLKV
                     VDNQVDQTTGTVKLKAEFPNAKLQLWPGQFVNVRLLVDTLKQVVVIPTGAVQRGPDGT
                     FVYVVKDDNTVAVRPIAVQKQDETQTVVGKGLEPAERVVTTGFVRLTDGTKVAIGSAD
                     APSAQPAGQRPQQGQGGGRRQQNGGSQKSNARQ"
     gene            complement(2315..3745)
                     /locus_tag="VFL49_01000"
     CDS             complement(2315..3745)
                     /locus_tag="VFL49_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET7910583.1"
                     /translation="MMLQSICVTLPLGGCLLAGDKPEPGLNIPPAYSAGPRNLAAAEA
                     KTPPLDWWRGFRSRELTGIVEEARAANLDIAAAVARIVQADANSRITGAALLPIVDLN
                     ANATRLRTSRSTSVGGTTGSLGGSERNLLTASLSASYEIDVWGKNRSAMRAAEELAVA
                     SRFDREVVALTTVVATANAYFQVLATQDRLRVAQDNLASATRVLNLIQQRLQAGTASA
                     LDTAQQESLVNTQRAAIPPLEQTLRLNRVTLAVLMARSPESVTIRGGSLRSIAYPRVT
                     PGLPSELLTQRPDIREAEANLAAANANVENARAQLLPSITLTGEGGYQSAVLRTLLRP
                     ESAFYTLAAGLTQPIFEGGRLLGNLDLQKGRQDELLQNYRKAVISGFADVENALDAIR
                     RTAERERLQREVVTSSRRAFDISEQRLREGTVDLVTVLQTQQTLFQAEDALAQARLAH
                     VLAVVSLYQALGGGWSPKPVEAADAR"
BASE COUNT          661 a         1233 c         1290 g          738 t
ORIGIN      
        1 tgaggaccac caggacgacc agcgcgatgc tgagtacgag ggtgaattgc acgtcctgaa
       61 tcgatgcgcg aattgtggtg gtgcggtcgt tgacgaccgt caggtccgcg cctgccggca
      121 tcgcacggcg caggcgcgga aactccgtgc gcaggcgctg caccgtctcg attacgttgg
      181 cgcctggctg ccgctgcacg tcgatgacaa ttgccggctg gccgcgatac caggcgccga
      241 ctttcgtgtt ctccaggccg tctacgacgt cggcgacatc gctgatcttg actggcgcgc
      301 cattgcggaa agcaatgacg atgtccctgt aggtgctggc ggccgtaatc tggtcgttgg
      361 ccgcgatggt gtacgattga tgggcgccat cgagcgcgcc ctttggcccg gcgacattgg
      421 cgccaaccac cgccgtgcgc acgtcatcga gtgcgatgcc gtaggcagca agccgcgaca
      481 ggtcggcctg aatgcgaatc gcgggccgaa tgccgccctg caccgagacg cgcccgacac
      541 ccgtcacctc ggacaggcgg ggtgccatga tggtgtccgc catgtcgctc agcgtgcgca
      601 ggtcgatggt cttcgacgtc agcgccagcg tgatgatcgg cgcgtcggcc gggttcactt
      661 tcgagtagag cggcgggtag ggaagattgc gaggcagcgt cgaagccgcg gcattgatcg
      721 ccgactgaac gtcctgcgcc gcggcgtcga tgtcgcggtt gaggtcgaac tgcagcgtca
      781 cctggctgat gccgaaggaa ctcgaagagg tcatcgtctg cagggcggga atttgcccga
      841 actggcgttc caacggagcc gtcaccagcg aggcgatcgt gtcgggattg gcgcccggca
      901 gctgcgtggt gacctggatg gtcgggaaat cgacctgcgg cagcgcggaa accggcagcc
      961 accaatagcc cagaaagccg cccagcatca ccgcgatgcc gagcaatgac gtcgcgatcg
     1021 gccggtggat aaatggcgac gatacgctca ttgtcgcgcg ttcgattttt gcgaaccgcc
     1081 gttttgctgc cggcggccgc caccttgccc ctgttgtggc cgctggccgg ccggctgcgc
     1141 cgacggcgca tcggcactgc cgatcgcgac cttggtgcca tcggtgagcc gcacgaagcc
     1201 ggtggtcacc acccgctcgg ctggctcgag gcccttgccg accaccgtct gcgtctcgtc
     1261 ctgcttctgc acggcgatcg gccgcaccgc aacggtgttg tcgtccttga cgacatagac
     1321 gaaggtgccg tctggaccgc gctgcacggc accggtcgga atcaccacga cctgcttcag
     1381 ggtatcgacc agcagccgga cgttgacgaa ctgcccaggc cagagctgca gtttggcgtt
     1441 gggaaattca gctttcagct tcaccgtgcc cgttgtctgg tcaacctggt tgtcgactac
     1501 tttcaataca ccgcggtcga tcaccgcatc attgtcggaa cgctgggcct cgacattgag
     1561 cggagatttg tcgaacgcgg tgtttacctg cgaaagatcc tgctgaggca gattgaacag
     1621 caccgaaatc ggctgcagtt gcgtgatgac cacgatcgac gaattggtgt cggccgcgcg
     1681 tacgtagttg ccttcgtcca ccatgcggat accggtgcgg ccatcgatcg gtgcggtgat
     1741 cgtggtgtaa ccaagcattg cctgggcatt ttcgatggcc gcctggtccg cctgtaccag
     1801 cgccgtgttc tgcgcgacca gcgcgcgctg ggtgtcggcc tgctgcttat tgatggcgtt
     1861 ggtggcggca agcctttcgt agcgctcgag atcgatcctg gcgttggcaa gctgcgcctc
     1921 atcctgagcc tttttcgcga tcgcctggtc gagctgcgcc cggtagatga cagggtcgat
     1981 ttttgccagc acatcgccct tcttgacatc gtcgccttcc ttgaagttga cgctgagaag
     2041 cttgccgtcg acctgcgggc gcacggtcac ggtattgaga gccttgatgg ttccgaccgc
     2101 gtccagaaag accggcacgt cggcccgtgt cgcggtggcg acgagcaccg gcacgggccc
     2161 ttcattgggc gtaaaacggc cgccgcgccg ttgctgcggc gctgactcac tgaagacgta
     2221 gtagagcgcc gcagcgacgg tgatcaggat aagagcgatc gcaaacgtgc ggcgcgtgcc
     2281 gctgcgtgct tttgccagaa tttgcttggg gaactcaccg tgcatcggcg gcttccaccg
     2341 gtttgggcga ccagccgccg ccaagcgcct gataaagact tacgaccgcc agcacatgag
     2401 ccagacgcgc ctgcgccagt gcatcctcgg cctggaagag ggtctgctgc gtctgcagca
     2461 cggtgacgag atcgaccgtg ccctcgcgca aacgctgctc cgaaatatcg aaggcgcggc
     2521 gcgagctcgt cacgacttca cgctgcagac gctcgcgttc cgctgtccgt cggatggcgt
     2581 cgagtgcgtt ctcgacgtcg gcaaatccgg aaatcacagc cttgcggtag ttctgcagca
     2641 gctcatcctg ccggcccttt tgcaggtcga gattgccgag cagccggccg ccttcgaaga
     2701 tcggctgtgt cagcccggcg gccaaagtgt agaacgccga ctccggacgc agcagcgtcc
     2761 tgagaaccgc gctttggtag ccgccttcgc cggtgagcgt gatactcggc aacagctgcg
     2821 cgcgtgcatt ttccacgttg gcgttggcgg cggcgagatt ggcctccgct tcgcggatat
     2881 cgggccgttg cgtaaggagt tcggagggca ggcccggcgt cacgcgcgga taggcgatgc
     2941 tgcgcaggct gccgccgcgg atcgtcacgc tttccggtga gcgcgccatc agcacggcca
     3001 gcgtcacgcg gttcaaccgc agcgtctgct cgagcggagg tatagccgcg cgctgggtgt
     3061 tgaccaagct ctcctgttgg gcggtgtcga gcgcggaagc cgtaccggcc tgtaggcgct
     3121 gctggatgag attgagcacc cgcgtggcgc tggccaggtt gtcctgggcg acgcgcaggc
     3181 ggtcctgcgt agcgagcacc tggaaatagg cattggcggt ggcaaccact gtggtcagcg
     3241 caaccacctc gcggtcaaag cggctggcaa cggcgagttc ttccgcagcg cgcatagccg
     3301 agcggttctt gccccagaca tcgatctcat agctggcgct cagggaggcg gtgagcagat
     3361 tgcgctcgga accgccgagc gaacccgtgg tcccgcccac agatgtcgac cgtgatgtcc
     3421 gcaatcgcgt ggcattggcg ttgaggtcca cgatcggaag cagcgccgcc ccggtgatgc
     3481 gcgagttggc gtcggcctgc acgatgcggg cgacggcagc agcgatgtca agattggcgg
     3541 cgcgggcctc ttcgacgatc ccggtcagtt cgcgcgagcg gaagccgcgc caccaatcga
     3601 gcggcggtgt tttcgcttcc gccgccgcga gattgcgcgg acccgcgctg taggcgggag
     3661 gaatattgag ccctggctcg ggcttgtcgc cggcgagcag gcagccgccc agcggcaatg
     3721 ttacgcaaat actctgcagc atcatccaga ccgggcgccc ccaaattttc cttcccgcct
     3781 ccttcgtctt cccaccccga tgcatcaaaa aagccttcaa atcctggcca agcaaacgac
     3841 tcggtcaagc aacaataata ctaccgtttg tccccccggg tctttcgtcc gggaggccaa
     3901 ttggccagcg cttgccgacc cg
//
LOCUS       DASSRL010000050         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_263653, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000050 DASSRL010000000
VERSION     DASSRL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263653"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFL49_01005"
     CDS             <1..701
                     /locus_tag="VFL49_01005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7910584.1"
                     /translation="GRGGALIEHQRLGVDPALAPAMYHEAFSVLMRAFEADVVDFEGR
                     FYNYKDYVTQMKPVQRPHPPIWYGAPNADAIAWAAPRGVNVVSLGPGTRARAISDRYR
                     EEWKKLGRDSTALPGIGITRHIVVADTDDEAQSIARAAYPCWREAIEHLWRRSGVDFV
                     LKEIYPADFGALERIGHGIAGSPATVRDYLTRLQSETGVNTVLCQIVFGDMRFEHAAH
                     SIRLFGRDIIPAFA"
     gene            773..2005
                     /locus_tag="VFL49_01010"
     CDS             773..2005
                     /locus_tag="VFL49_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7910585.1"
                     /translation="MIVRKGEAHGGVDSPAAWFRLVVAMAISTIGGVGMWSIMVVLPT
                     IQADFGVSRADASLPFTMVMFGFAGGGVLMGRLADRFGIAVPIALGTLAVSAGYLATG
                     WSSSLWQVAGAHLLIGIGCSSTFGPLMADMSHWFVRRRGIAVALAAVGNYVAGTIWPP
                     VLQHFIATSGWRVTHIGVGLFCLVTMMPLLLLMRRRIDDHHADEAGAAAAQRQANMPV
                     SPRTLQILLCIAGVCCCVAMAMPQVHIVAYCGDLGYGVARGAEMLSMMLGFGIISRVG
                     SGFIADRIGGVATLLLGSILQGTALFLYLWFDGLASLYVISALFGLFQGGIVPSYAII
                     IREYFSPREAGTRLGIVLMATLLGMALGGWLSGLIFDLTGSYRAAFFNGLAWNLVNVS
                     IMVWLLQRSRRRGQLAAA"
     gene            complement(2011..>3188)
                     /locus_tag="VFL49_01015"
     CDS             complement(2011..>3188)
                     /locus_tag="VFL49_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7910586.1"
                     /translation="LSQNIIHLVLARIEGAPAGTKGISLFVVPKFLVKDDGSVGARNA
                     VTCGSIEEKMGIHGNSTCVMNYDGATGWLIGEENRGLNAMFTMMNEARLGVGVQGLAL
                     SEVAYQNAAIYAKERLQGRAISGVKYPDKPADPIIVHPDVRRTLMTIRAFNEAARALV
                     IWTALKSDIARRSDNEKDRKSADDHMGLLTPVIKGVLTDSGFANAVMAQQVYGGHGYI
                     AEHGMEQFVRDSRIAQIYEGANGIQALDLVGRKLGKDGGRAIMAFFNEVQAYLKERTN
                     NDAMNVYLKPLGQSLAHLQEASMWFMQNAMTKPDNAGAGSYDYMHLFGLVALGYMWCL
                     IAEAAIAKKGNGAMPDMDAKLVTARFFMDRVLPETATRLARIKAGAGSTMELPDDAF"
BASE COUNT          509 a          996 c         1018 g          665 t
ORIGIN      
        1 tgggccgcgg cggagcattg atcgaacatc agcgtcttgg cgtcgacccg gcgctggcgc
       61 ccgcgatgta tcacgaggcg ttcagcgtgc tgatgcgcgc ctttgaggcc gatgtggtcg
      121 atttcgaagg ccgcttctac aactacaaag attatgtgac gcagatgaaa ccggtgcagc
      181 gcccgcaccc gccgatctgg tacggtgccc caaacgcaga tgcgattgca tgggcggcac
      241 ctcgcggcgt gaatgtggtg tcgctcggtc ccggcacccg cgcgcgggcg atcagcgatc
      301 gctatcgcga ggaatggaaa aagctcggac gcgacagtac cgcgctgcca ggcatcggaa
      361 tcacccggca tatcgtcgtc gccgataccg acgacgaagc gcaatcgatt gcacgcgccg
      421 cctatccatg ctggcgcgaa gccattgagc acctctggcg gcgctcgggc gtcgatttcg
      481 tgctgaagga aatctacccg gccgatttcg gcgcgctcga gcgcatcggt cacggcattg
      541 ccggctcgcc cgcgactgtg cgcgactatc tgacccgcct gcaatccgaa accggcgtca
      601 acaccgttct atgccagatc gtgtttggtg acatgcgttt cgaacatgcc gcccattcga
      661 tccggctgtt cggccgcgac atcattccgg cattcgcata gcggatggcg ggctgtctgg
      721 aatacgcctg cgcgcagtga taccacgcct gtgcaatgcc gggtctccaa gcatgattgt
      781 acggaagggt gaagcgcacg gcggtgtcga ttcgccggcc gcatggttcc gacttgtcgt
      841 ggcgatggca atttccacca tcggcggagt cggcatgtgg tcgatcatgg tggtcttgcc
      901 aacaatccag gccgacttcg gcgtgagccg cgccgacgca tcactgccct tcaccatggt
      961 gatgttcggc tttgccggcg gcggcgtcct gatggggcga ctggcggacc ggttcggcat
     1021 cgccgtgcca atcgcgctcg gaacccttgc ggtgtcggcc ggctacctcg cgaccgggtg
     1081 gtcatcaagc ctgtggcaag ttgcgggtgc gcacctgctg atcggcatcg gctgctcctc
     1141 caccttcggc ccgctgatgg ccgatatgtc gcactggttt gtgcgccggc gcggcatcgc
     1201 ggtggcgctg gccgccgttg gaaattacgt cgcaggcacg atctggccgc cggtattgca
     1261 gcatttcata gccacatccg gctggcgcgt aacccatatc ggcgtcggct tattctgcct
     1321 cgtgacgatg atgccgctgc tgctcctcat gcgccggcgg atcgacgatc atcacgcgga
     1381 tgaggcaggc gcggccgccg cgcaacggca ggccaatatg ccggtttcac caaggacctt
     1441 gcagattctc ctgtgcattg ccggagtttg ttgctgcgtg gcaatggcga tgccgcaagt
     1501 gcacatcgtc gcctattgcg gcgatctcgg ctatggcgtg gcgcgcggcg ccgaaatgct
     1561 gtcgatgatg ctcggcttcg gaatcatcag tcgcgttggc tccggcttta tcgccgaccg
     1621 catcggcggc gtggcgaccc tgctgcttgg atcgatcctg caaggcaccg ccttgtttct
     1681 ttatctctgg ttcgacggcc tcgcgtcgct ctacgtcatc tcggcgctgt tcggcctgtt
     1741 ccagggcggc atcgtgccga gctatgccat catcatccgc gaatattttt caccgcgcga
     1801 ggcgggcacg cgtctcggca tcgtgctgat ggcgacgctg ctcgggatgg cgctcggcgg
     1861 ctggctgtcc ggcctgatct tcgacctcac cggttcctac cgcgcggcgt ttttcaacgg
     1921 gctggcgtgg aacctggtga atgtgtcgat catggtgtgg ctgctgcagc gctcgcggcg
     1981 gcgcgggcaa ttggcggcgg catagccact tcagaacgcg tcgtccggca gttccatcgt
     2041 cgagccggcg cccgccttga tgcgtgcgag ccgcgtggcg gtttccggca gcacgcgatc
     2101 catgaaaaag cgcgcggtga cgagcttggc gtccatgtcc ggcatcgcgc cgtttccctt
     2161 tttggcgatc gcggcctcgg cgatcagaca ccacatgtag ccgagcgcca ccaggccgaa
     2221 gagatgcatg taatcgtatg agccggcgcc ggcgttgtcg ggcttggtca tggcgttctg
     2281 catgaaccac atcgaggctt cctgcaggtg cgccaatgat tggccgagcg gcttgagata
     2341 gacgttcatc gcatcgttgt tcgtgcgttc cttgagataa gcttgcacct cgttgaagaa
     2401 ggccatgatg gcgcggccgc cgtccttgcc gagcttgcgg ccgacgaggt cgagcgcctg
     2461 gataccgttg gcgccttcgt agatctgcgc gatgcgggaa tcgcgcacaa actgctccat
     2521 gccgtgctcg gcaatgtagc cgtgaccgcc gtatacctgc tgcgccatca ccgcgttggc
     2581 aaagccgctg tcggtgagga cgcctttgat caccggcgta agcaggccca tatggtcgtc
     2641 ggcgctcttg cggtcctttt cattgtccga gcggcgggcg atgtcgctct tgagcgcggt
     2701 ccagatcacc aatgcgcgcg cggcctcgtt gaaggcacgg atggtcatca gcgtccgtcg
     2761 cacatccgga tgcacgatga tcggatcggc cggcttgtcg gggtatttca cgccggagat
     2821 ggctcggccc tgcaggcgtt ccttcgcgta gatcgccgcg ttctggtagg cgacctccga
     2881 cagcgccagc ccctgcacgc cgacgccgag gcgcgcctcg ttcatcatcg tgaacatggc
     2941 gttgaggcca cggttttcct cgccgatgag ccagccggta gctccgtcat aattcatcac
     3001 gcaggtcgaa ttgccgtgga tacccatttt ctcttcgatc gaaccgcagg tgaccgcatt
     3061 gcgtgcaccg accgagccgt cgtcctttac gagaaacttc ggcaccacga acaaagagat
     3121 gcctttcgtg ccggcaggcg cgccttcgat gcgggccagc accagatgga tgatgttctg
     3181 cgaaaggt
//
LOCUS       DASSRL010000051         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1138898, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000051 DASSRL010000000
VERSION     DASSRL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138898"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>2425
                     /locus_tag="VFL49_01020"
     CDS             21..>2425
                     /locus_tag="VFL49_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7910587.1"
                     /translation="MKDFEEMIVRAMPNGSMIRLKDVARIELGAQSYDLEGRYRGKPT
                     ALLVTFLAPGFNALDTIKHIRERMDELSKTFPPGVSYDVPYDTTRFIEVSIEEVVKTL
                     RDAMILVILVVYLFLQSWRATLIPAVAVPVSLIGTFAGMSALGFSINTLTLFGMILAI
                     GIVVDDAIVVVENVERHIEDGESPKEAAKKAMDEVTGPVIAIVLVLAAVFVPVGFLGG
                     ITGQLYKQFAITIAISVAISGFVALTLSPALCALILKPRQAPRKGVFAYFNRFFGWAQ
                     TRYAGAVGHTIGRPLLSLALFGIVGFMAFTLFQLTPSGFLPDEDQGYIIVVAQLPDGA
                     SKKRTLAVMDQVEGFFTSNPAVYGTNALVGQNFVFNTRGTYMATTFVPLIPWNERKDP
                     QMHAKALIAGAFKEFGKFRDAVVLAFNPPSIRGLGATGGFSVQIQDPSGGDFKKFAAV
                     TREFIGKAKQEPAIGGINTSFRVTAPRLYATINRDRAKALGIPISEVFDTMQAYFGNF
                     YINDFVKFGRVYRVQTEAEAQYRSAPQDISKVYVRAMNGQGQGHAMIPLDTVVSIESI
                     SGPDPVTHFNGFNTALVLGASSSGSSSGQTLDALQRVAQEVLVPQGYRIDWSGISFQE
                     RKAGAQSVLAFAFGLLMVFLVLAAQYESWTVPFAVILAVPFGVFGALSAVWLRSMNND
                     IYFQIGLVTLIGLSAKNAILIVEFANQEYHHGRPLIESAIDAARLRFRPIIMTSMAFI
                     LGVLPLVIASGAGAASRRSIGTGVLGGMLAATFLAIFFVPLFFVLIGKMNQWVTGRGP
                     TEPS"
BASE COUNT          443 a          739 c          718 g          525 t
ORIGIN      
        1 cacgcggccg tctgaccgac gtgaaagatt tcgaggaaat gatcgtccgg gcgatgccca
       61 acggctccat gatccggttg aaagacgtgg cccggatcga actcggcgcg caatcctacg
      121 atctggaagg gcgctatcga ggaaagccca cagccctgct cgtcacgttt ctggcgccgg
      181 gcttcaacgc gctcgatacg atcaagcaca tccgcgagcg catggacgaa ctgtcgaaga
      241 cgtttcctcc cggcgtttcg tacgacgtgc cctatgacac gacccgattc atcgaagtgt
      301 cgattgaaga ggtcgtcaag acgctgcgcg atgcgatgat cttggtcatt ctcgtcgtct
      361 atcttttttt acagtcctgg cgtgcgacgt tgattccggc ggtagccgtg ccggtttcgc
      421 tcatcgggac ctttgccggc atgtccgcgc tgggcttctc gatcaatacg ctgacgctct
      481 tcggcatgat cctggccatc ggcatcgtgg tggacgacgc catcgtcgtc gtcgagaacg
      541 tcgagcgcca tatcgaagac ggtgagtcgc ccaaggaagc ggccaaaaag gccatggacg
      601 aagtgacagg tcccgtgatt gccatcgtgc tggtcctggc tgcggtcttt gtgccggtgg
      661 gctttttagg cgggattacc ggccagttat acaagcaatt cgcgatcacg atcgcgatct
      721 ctgtcgcgat ctccggcttc gtcgcgttga cgctgagccc cgcgctctgc gccttgatcc
      781 tcaaaccgcg tcaggctccc aggaaaggag tgttcgcgta cttcaatcgc ttcttcgggt
      841 gggcccaaac acggtatgcc ggcgccgtgg gccatacgat cggacgtccg ctgctctcgc
      901 tggccttgtt cggcatcgtt ggctttatgg cgtttactct cttccaactc accccctcgg
      961 gttttttgcc tgacgaagac caaggctaca tcatcgtggt cgcccagctt cccgacggcg
     1021 cctcaaaaaa acggacgctt gccgtgatgg atcaagtcga aggatttttt acttcgaatc
     1081 cggccgtcta tggcacgaat gccttggtcg gccagaattt tgtgttcaac acccgcggga
     1141 cctacatggc gacgaccttc gttccgctca tcccctggaa tgaacggaag gacccgcaga
     1201 tgcatgcgaa ggcgctgatc gccggggcct tcaaggagtt cggaaaattc cgcgacgccg
     1261 tcgtcttggc ctttaaccct ccgtcgatcc gcggcctcgg ggcgaccggc ggattctccg
     1321 tccagatcca ggatcccagc ggcggcgact ttaaaaaatt tgccgccgtg accagggagt
     1381 tcattggcaa ggcgaagcaa gagccggcga tcggcggcat caacaccagc ttccgtgtga
     1441 ccgccccgag gctgtacgcc acgatcaacc gcgaccgcgc caaagctctc gggatcccca
     1501 tttctgaagt gttcgatacg atgcaagcgt acttcgggaa tttctacatc aacgatttcg
     1561 tcaaattcgg ccgggtctat cgggtgcaaa ccgaggcgga agcccaatat cgttcggcgc
     1621 cccaggacat ttcgaaagtg tatgtgcgcg cgatgaatgg ccagggccaa ggccatgcca
     1681 tgattccgct cgataccgtg gtgagcatcg aatcgataag cggcccggat ccggtcacgc
     1741 actttaacgg attcaacacc gccctggtcc tcggcgcatc cagctcgggc tccagctccg
     1801 ggcaaacgct ggatgcgctg caacgggtag cacaggaggt gttggtgccg caaggatatc
     1861 gcatcgactg gagcgggatc tccttccagg agcggaaggc gggcgcccaa tcggtgctgg
     1921 ccttcgcgtt tggcctcctc atggtatttc tggtcctcgc ggcgcagtac gaaagctgga
     1981 ccgtgccctt tgccgtgatt ctcgccgtgc ccttcggcgt cttcggcgcc ttgtcggcgg
     2041 tgtggctgcg cagcatgaac aacgacatct atttccagat cgggctcgtc acgctgatcg
     2101 gactctccgc gaagaatgcc atcctgatcg tcgaatttgc caaccaggag taccaccatg
     2161 gccgcccgtt gatcgaatcg gcgatcgacg cggcgcggct gcgcttccgc cccattatca
     2221 tgacgtcgat ggccttcatt ctcggcgtgt tgccgctggt gatcgccagt ggggccggcg
     2281 ccgccagccg gcgatccatc ggcacgggtg tgctgggcgg catgctcgcg gcgacatttc
     2341 tcgccatctt tttcgtgccg ctgttctttg tgctgatcgg aaaaatgaac cagtgggtca
     2401 ccgggcgggg gccgacggag ccatc
//
LOCUS       DASSRL010000052         7441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_36633, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000052 DASSRL010000000
VERSION     DASSRL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36633"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..429)
                     /locus_tag="VFL49_01025"
                     /pseudo
     CDS             complement(1..429)
                     /locus_tag="VFL49_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
     gene            499..1716
                     /locus_tag="VFL49_01030"
     CDS             499..1716
                     /locus_tag="VFL49_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HET7910588.1"
                     /translation="MTLIRPFRALRPASGRAAEVLAPPYDVLSSAEARIRAKGKPWSF
                     LHISKPEIDLDPATDPYDKSVYAKSAENLKSMMAADVLTRDDKPCYYVYRLTWRDRVQ
                     TGLAAIASLKDYAINRIRKHEHTTPTKEDDRVRQIEAINAQTGPVMSGYPNAPAIDAM
                     LAQTAAGKPEIDVTADDGVRHQLWTVADDTTIAKLTRAFDALPAIYIADGHHRSAAAA
                     RVAAARKGDGSHGYFLSVIFPEHEMTILDYNRVVKDLNGRSPEQFLAELQKSFAVTSS
                     NQPVRPASAHDYGMYLNNRWYKLSVRPELVSTDPIGRLPITVLTRNIIAPLLGIDDPR
                     TDKRIDFVGGARGLEELERRVKSGEMAVAFSLYPTQMRDLMAVADAGGIMPPKSTWFE
                     PKLADGMVSHVLD"
     gene            complement(1747..3246)
                     /locus_tag="VFL49_01035"
     CDS             complement(1747..3246)
                     /locus_tag="VFL49_01035"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET7910589.1"
                     /translation="MGANAFCPNTRLGRAGLAVTTAAAVVFFAITAPAAARGPEGIAD
                     VAEQVIDAVVNVSTKQSVDLRSGAMPQLPPGSPFEEFFEEFFKNRRGQNGPGQNNQSP
                     TPRRVNSLGSGFIIDPSGIVVTNNHVISEADEVSVILNDGTTLKAEVIGRDQKTDLAL
                     LKVAPTKPLKAVKFGDSDKLRLGEWVIAIGNPFSLGGTVTAGIVSARNRDIQSGPYDN
                     YIQTDASINRGNSGGPLFNLNGEVIGVNTAIISPSGGSIGIGFAVPSKTVVPVVDQLR
                     EFKEVRRGWLGVRIQQVSDEIAESLSVKPARGALVAGVDDKGPAKPAGIEPGDVIVKF
                     DGKDVKEMRDLPKIVADTPVGKDVEVVLIRKGKEEKKSVKLGRLEDEKKVAVATDKKD
                     ATPESKPVVKKALGLDLANLTDDLRKKHNIKDKVKGVLITGVDPNSPASEKRLAPGMV
                     IAEVQQQPVSNANELQQRIDKLKKDGKKAVALLVVSPDGDPSFVALSLQ"
     gene            complement(3352..3537)
                     /locus_tag="VFL49_01040"
     CDS             complement(3352..3537)
                     /locus_tag="VFL49_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HET7910590.1"
                     /translation="MSDFLAALGLVFVIEGLIFAAFPKAAKRAMTSVLETQDLSLRLI
                     GIGSAIIGLILVWLVRG"
     gene            complement(3557..4474)
                     /gene="hflC"
                     /locus_tag="VFL49_01045"
     CDS             complement(3557..4474)
                     /gene="hflC"
                     /locus_tag="VFL49_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET7910591.1"
                     /translation="MRTNFVGGIFAVVVVAALIVGYSSLFTVYQTQQALVIRLGEPRP
                     PVPNPGLHVKAPFIDSVVYIDKRILDLEAPPQEVIASDQKRLVVDAFARYRIKDPLRF
                     YQTLGSINGANSQLSILLNSALRRVLGEVTFTHVVRDQRADLMARIRELVDHEAAAYG
                     IEVVDVRIRRADLPEQNSQAVYQRMQTERQREAAEFRAQGAQRAQEIRSRADREVTVL
                     VADATSKSEQIRGEGDATRQQIFAEAFNQDPDFFSFYRSMQAYEQGLKHNDTRMLLKP
                     DSNFFRYFTDPSGKQRENSATPAPSAPAR"
     gene            complement(4471..5607)
                     /gene="hflK"
                     /locus_tag="VFL49_01050"
     CDS             complement(4471..5607)
                     /gene="hflK"
                     /locus_tag="VFL49_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET7910592.1"
                     /translation="MPWTNQGGGGPWGSGGKGPWGSGPQSPGPTPPDLEEILRRGQDK
                     LRRVLPGGNLGGRGFALIVLGVLVLWGFSGFFRVEPDELGVVLRFGKQVREVQPGLNY
                     HLPYPIEKVLTPKALRVNKIDIGMRIVDDLRRGGTTTRDVPEESLMLTGDENIVDVDF
                     SVLWKVKPTGVGDYLFNIQNPEGTVKAVAESAMREVIGRSEIQPILTGARQTIENAVQ
                     ELMQRTLDHYGAGILVQQVQLQKVDPPAQVIDAFRDVQAARADLERAVNEAQTYANRV
                     VPEARGRVAQITQAAEAYKQQTVAEATGQTARFLKIYEQYKKAPDVTRERMYLETMER
                     VLGGTDKIILDSGAGGSSVVPYLPLNELTRPAQPRSQSQSGGRQ"
     gene            complement(5720..6223)
                     /locus_tag="VFL49_01055"
     CDS             complement(5720..6223)
                     /locus_tag="VFL49_01055"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7910593.1"
                     /translation="MKIVLVAAIGDNLVIGREGQLPWRLKSDLQHFRKVTLNRPVIMG
                     RKTHESIGKLLGGRTNIVLTRDLSLVAPGAVLATSLDAALGVARADAAKRGVDEIMVI
                     GGSDIFAATMAMADRLEITHVRASPEGDAAFPPIDDEIWQEASREEHFAGPDDDADFA
                     FATYTRR"
     gene            complement(6432..7226)
                     /locus_tag="VFL49_01060"
     CDS             complement(6432..7226)
                     /locus_tag="VFL49_01060"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7910594.1"
                     /translation="MRQYLDLMELILSRGVEKRDRTGTGTLSVFGHQMRFALGEGFPL
                     VTTKRLHLKSIIHELLWFLAGDTNVRYLREHGVRIWDEWADERGELGPVYGQQWRSWP
                     AQDGKTIDQIGNVVTMIRRNPDSRRLIVTAWNPADVDKMALPPCHCLFQFYVAEGRLS
                     CQLYQRSADVFLGVPFNIASYALLTQMMAQVTGYKPGDFIHTLGDAHLYLNHLDQARL
                     QLSRAPKPLPTMRLNPAVKDIFAFRYEDFSIENYDSHPHIKAAVAV"
BASE COUNT         1494 a         2285 c         2132 g         1530 t
ORIGIN      
        1 acgccggcgc gaatgtcggc gggaattggc ggaacggcgg cgaggcctga cgtcagagcc
       61 ttgaaataaa gtccggtccc tccaaccagg atcggcacgc gtccttgttt gcggatctcc
      121 gtcagtgcct cccaccagga tcggcacgcg tccgtgtttg ccaatctcct tgagcgtccc
      181 ttcgacgtcg cgacaccagc ggccgaccga ataattctcg gccgcatcga cgaacccgta
      241 caacttgtgc ggtgcccgga tctcgtcctc agccgtcggc cgggcggtaa tgactcgcag
      301 atcgcggtaa acctgcatgg aatcggcatt aatgatagcg ccgcgcaggc attcagcgac
      361 atcaagcgcc agtgccgact tgccgctggc agtcggccct gcaataagca ccgctcgcac
      421 tgtggtcata gtgccgcctt tgttgacggc gtgatagcac cggcaggacg acaactcacc
      481 cgatgtttgg agacggactt gacactcatc agaccatttc gggcgttgcg gcccgcgtcc
      541 ggtcgcgccg cggaggttct ggcaccgccc tatgatgttc tttccagcgc cgaggctcgc
      601 atccgcgcca aaggcaagcc atggagcttc ttgcatattt cgaaaccgga gatcgacctc
      661 gatccggcaa ccgatccgta cgacaagagc gtctacgcta aatcggctga aaacctcaaa
      721 agcatgatgg cggcggacgt tctaacccgc gacgacaaac cttgctacta tgtctatcgg
      781 ctcacttggc gtgaccgtgt tcaaacgggt ctggccgcca ttgcctcgct taaggactat
      841 gcgatcaatc gcattcgaaa gcacgagcac acgacgccaa caaaagaaga tgatcgtgtt
      901 cggcagattg aggccatcaa tgcccaaacc ggtcccgtga tgagcggcta tccgaatgcg
      961 ccggcgattg acgccatgtt ggcgcagacg gccgccggca aaccggaaat cgatgtcacg
     1021 gccgatgatg gcgtgcgcca tcagctttgg acggttgccg acgacacgac aatcgccaag
     1081 ctgactcgcg ctttcgacgc gctgcccgct atttacattg ccgatggcca tcaccggtca
     1141 gcagcggctg cgcgtgtcgc ggccgcccgt aagggcgatg gctcgcatgg atatttcctg
     1201 tcggtcatct ttccagagca cgaaatgaca atcctggact acaatcgggt ggtgaaagat
     1261 ctgaacggcc gcagccctga gcaatttctc gccgaactac agaagagttt cgcggtaacc
     1321 tcttccaatc agccggtacg acctgcctcc gcgcatgact atgggatgta tctgaacaac
     1381 cgctggtaca aactttcagt ccggccagaa ctggtttcaa ccgatccgat cggacgactg
     1441 ccgatcaccg ttctcacccg caacatcatt gcgccactgc tcggcatcga tgatccgcgt
     1501 accgacaagc gcatcgattt cgtcgggggc gcgcgcggcc tggaagaact ggaaaggcgc
     1561 gtaaaatcag gcgagatggc tgtcgcgttc tcgctctatc cgacgcaaat gcgagatctg
     1621 atggcagtgg ctgacgccgg cggcatcatg ccgccgaaat ccacctggtt tgagcccaag
     1681 ctcgctgacg gcatggtcag tcacgtgctc gattgacgaa agcgccggtg cggcgccttt
     1741 cgctgactac tgcaagctca gcgccacgaa gcttggatcg ccgtcaggag atacaacgag
     1801 cagcgcgacg gcttttttgc cgtctttctt gagcttgtcg attcgctgct gcagttcgtt
     1861 ggcatttgag acgggctgct gctgaacctc ggcgatgacc atgccggggg cgaggcgctt
     1921 ctctgaagcc ggtgaattcg ggtcgacccc ggtgatcagc acccctttta ccttgtcctt
     1981 gatgttgtgc tttttgcgca ggtcatcggt gagattggcc aggtcgaggc caagcgcttt
     2041 cttcaccacc ggcttgctct cgggcgtggc atccttcttg tcggtggcga ccgcgacctt
     2101 cttttcatct tcgaggcggc caagcttgac cgacttcttt tcctctttgc ctttgcggat
     2161 gaggacaacc tcaacgtcct tgcccaccgg cgtatcggca acgattttcg ggaggtcccg
     2221 catttccttg acgtccttgc cgtcgaattt gacgatgacg tcacctggct caattcctgc
     2281 aggcttggcc ggacccttat catcgacgcc ggccacgagc gcaccgcgcg ccggcttgac
     2341 gctaaggctt tccgcgatct cgtcgctgac ctgctgaatg cggacaccaa gccatccgcg
     2401 ccgtacctct ttgaactcgc gcagttgatc gacaaccgga accacggtct tcgaggggac
     2461 cgcaaaaccg ataccgatgg atccgcccga tggcgagatg atcgcggtat taacgccgat
     2521 cacttcgcca ttgaggttga acagcggtcc gccggaatta ccgcggttaa tcgaggcgtc
     2581 agtctgaatg tagttgtcgt aaggccccga ttgaatatcg cggttacgcg ccgagacgat
     2641 gccggcggtc accgttccgc ccagactgaa cggattgccg atggcgatca cccactcgcc
     2701 cagacgcagc ttgtcactgt cgccgaactt cacagctttc agcggctttg tcggcgcgac
     2761 cttgagcaac gcgagatcgg tcttctgatc gcggccaatc acttcggcct tcagcgtcgt
     2821 gccgtcattg agaataacgc tgacctcgtc cgcttcggaa atgacgtggt tgttagtcac
     2881 gacaattccg ctcgggtcaa tgataaagcc cgaaccaagc gaattgacac gacgaggtgt
     2941 cggcgactga ttgttctgtc ccgggccgtt ctgtccgcgc cggttcttga aaaactcttc
     3001 gaagaattcc tcaaacggcg atcccggtgg caattggggc atggcgccac ttcgcaggtc
     3061 gaccgattgt ttggtcgaaa cattcaccac ggcgtcgatg acttgctcag ccacatcggc
     3121 gattccctca ggtccgcgcg cggccgccgg agcagttatc gcgaaaaaga cgacggctgc
     3181 cgcggtggtc acggccaagc cggcccgacc gagacgagta ttcgggcaga atgcgtttgc
     3241 gcccatgggc gtcagttctc ctgggaatag gcctgttcga gagagtggcc ccgacttgta
     3301 ggcgattcaa gggcaccgcc aatcaatacc atgcgaggcc gggtttccct atcagccccg
     3361 caccagccag accaaaatca atccaataat cgccgacccg ataccgatca accgcaggga
     3421 gaggtcctgc gtctccagca cgctggtcat ggcccgcttg gccgccttgg gaaacgccgc
     3481 gaagatcagt ccctcgatga caaaaaccag tccgagcgcg gccaggaaat cggacatgcc
     3541 ggggccggcg cagccgctat cgcgccgggg ccgagggggc gggcgtggcc gaattctccc
     3601 gctgttttcc ggaagggtcg gtgaaatagc ggaagaagtt cgaatcgggc ttcagcaaca
     3661 tgcgtgtgtc gttgtgcttg agtccctgct cgtaggcctg catagaccgg taaaaggaga
     3721 agaaatccgg gtcctggttg aaggcttccg cgaaaatctg ctgtcgcgtc gcgtcgccct
     3781 cgccgcgaat ttgttccgat ttcgaggtcg catcggccac gagcacggtc acttcgcggt
     3841 cagcccgcga gcggatttcc tgcgcgcgct gcgcaccctg cgcgcggaac tcagccgctt
     3901 cgcgctgccg ttcggtctgc atgcgctgat agacagcctg gctgttttgc tccggcaaat
     3961 ccgcgcggcg gattcgcaca tcgacgacct cgatgccata ggcagcggct tcgtggtcaa
     4021 cgagctcgcg aatacgagcc atcagatcgg cacgctgatc tcgcacgacg tgtgtgaatg
     4081 tcacttcacc gagcacccgt cgcagcgccg aattgagcag gatagacaat tgcgaattcg
     4141 cgccgttgat tgaacccagg gtctggtaga aacggagcgg gtccttgatc cggtatcggg
     4201 cgaatgcgtc gaccacaagc cgtttctgat ccgacgcaat cacctcttgc ggaggcgctt
     4261 ccaaatcaag gatgcgctta tcgatgtaaa ccactgaatc gataaatggc gccttcacat
     4321 gaagacctgg attgggaacc ggcggccgcg gctcgcctaa cctgataacc agcgcctgct
     4381 gggtctgata gacggtgaac agcgacgaat aaccgacaat cagcgcggca acgaccacaa
     4441 cagcgaagat gccgccgaca aaatttgttc tcattgccgg cctccgctct gcgactggct
     4501 gcgcggctgg gccggccggg taagctcatt gagcggcaga tagggcacta cgctgcttcc
     4561 gccggcgcct gagtccagga taatcttgtc ggtgccgcca agcacgcgct ccattgtttc
     4621 aagatacata cgctcgcgcg tgacgtcggg cgccttcttg tattgctcgt aaatcttgag
     4681 aaaccgcgcg gtctgaccgg tcgcttcggc gacagtctgc tgcttgtagg cttcggcggc
     4741 ctgcgtgatc tgtgcaactc gtccacgcgc ttccggcacc acgcggttgg cgtaggtctg
     4801 cgcttcgttg acggcacgct cgagatccgc gcgtgcagcc tgaacgtcgc ggaaggcatc
     4861 gatcacctgc gccggcgggt cgactttctg caactgtacc tgctgcacca aaatgccggc
     4921 accgtagtgg tcaagcgtcc gctgcatcaa ctcctgcacg gcattttcga tggtttgccg
     4981 cgccccggtc aggatcggct gaatttcaga gcggccgatc acttcgcgca tcgcgctttc
     5041 ggctacggct ttcaccgtac cctcgggatt ctggatgttg aacaggtaat cgccgacgcc
     5101 cgtcggctta accttccata aaaccgaaaa atcaacgtcg acgatgttct cgtcgccggt
     5161 cagcatcaag ctttcctcgg gcacatcgcg ggtcgttgtg ccgccgcgac gaaggtcatc
     5221 gacgatccgc atgccgatat cgatcttgtt gacgcgcaag gctttcggcg tgagcacttt
     5281 ctcgatcgga tacggaagat ggtagttcag gcccggttga acctcccgga cctgcttgcc
     5341 aaagcgaagc acaacaccga gttcgtccgg ttcaacccga aaaaatcccg agaagcccca
     5401 aagcaccagc acgccgagca cgatgagagc gaatccgcgt ccgcccaaat tgccgccagg
     5461 caggacgcgg cgcagcttgt cctggccgcg gcgcagaatt tcttcgagat ccggcggtgt
     5521 cggccccggc gattgcggtc ccgagcccca tggccctttg ccgcccgagc cccagggccc
     5581 accgcccccc tgattggtcc aaggcattga aaatgtctcc cggcgccagc cgtacagccg
     5641 gggcgcctgt tgtcgggagg gttatagggg accgtaggcg cggttacaac gcgctgtcat
     5701 gccgcgcatt aacgacgttt caacggcgtg tatatgtcgc gaacgcgaag tcggcgtcat
     5761 cgtcagggcc ggcaaagtgc tcctcgcgtg atgcttcttg ccagatttca tcatctatag
     5821 gtggaaaggc tgcgtctccc tcgggcgatg cgcgtacatg cgtaatttcc aaccggtccg
     5881 ccatcgccat ggtggcggca aaaatatcgc tcccgccaat caccataatt tcatcgactc
     5941 cgcgttttgc cgcgtccgcg cgcgcgacgc ccaatgccgc gtcgagactc gttgccagca
     6001 cggcgcccgg agccaccaaa ctcaggtcac gggtgaggac gatattggtg cgccctccaa
     6061 gcagcttgcc gatcgactcg tgcgtcttgc ggcccatgat caccggacga ttgagagtca
     6121 ctttgcgaaa atgctgaagg tccgatttca ggcgccaagg caactggcct tcacggccaa
     6181 tcaccaggtt gtcgccgatc gctgccacga gcacgatttt catgaggcct gcgatgaaac
     6241 tgaattgcgg gacattggca ttgctttccg acaatacgaa taagccaaaa ccgttctgac
     6301 tttattacca acgtccggca catcgtgcga aacaaattcc gaaattacgc aaagaagttc
     6361 ccgcgttaaa atcagcttag cgcgacgcct ctaaacggca gcaacgccga aatcgctttt
     6421 tggcattcgg atcacaccgc cactgcggcc ttgatgtgcg ggtgcgaatc gtaattttcg
     6481 atcgaaaagt cttcatagcg gaacgcgaat atgtccttca cggcgggatt gagccgcatc
     6541 gtcggcagcg gcttcggcgc gcgcgacaat tgcaagcggg cctgatcgag atggttcagg
     6601 tacaagtgcg cgtcacccag cgtgtgaatg aaatcgcccg gcttgtagcc tgtcacctgt
     6661 gccatcatct gcgtgagcag cgcataagat gctatgttga atggcacacc gagaaaaaca
     6721 tccgccgaac gttggtagag ctggcacgac agcctgcctt cggcaacata gaactggaaa
     6781 aggcagtggc aaggtggcaa tgccattttg tcgacgtcgg ccggattcca cgcggtcacg
     6841 atcagccgcc gcgagtccgg attgcgcctg atcatcgtaa cgacattacc gatctggtcg
     6901 atggtcttgc cgtcctgcgc cggccatgat cgccactgct ggccataaac gggtcccaac
     6961 tcgccgcgct cgtccgccca ttcgtcccaa atgcgcacgc catgctcccg caggtaccgg
     7021 acgttggtat cgccggccaa aaaccacagg agttcatgga tgatcgattt caggtgcagt
     7081 ctcttggtcg tcaccagggg aaagccttcc cctaacgcaa accgcatttg gtgaccgaac
     7141 accgacagtg ttccggtgcc ggtgcggtcg cgtttctcga cccctcggct aaggatgagt
     7201 tccatcaggt caagatattg gcgcatcttt ccggatatcg gctggcgact cgaagatgcg
     7261 gaagtctatc gccgggagca acggatggca gtgctttttc aaccgtccag atggcagccc
     7321 cggcgcccga ctcctgatac aatagtttcg ctgcagttgg ggtacgccat gggcttcgat
     7381 cgcattcttg ttgcccggat tctcttcaca ctcatgaccg caggctgggc gctgctcaca
     7441 g
//
LOCUS       DASSRL010000053         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_754039, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000053 DASSRL010000000
VERSION     DASSRL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754039"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFL49_01065"
     CDS             complement(<1..360)
                     /locus_tag="VFL49_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET7910595.1"
                     /translation="MSKRSLTFVCQNCGAAFSRWQGKCEACGEWNTLSEEGSERPSGP
                     GRKPGKGRLFALEALTGEAHDAPRLVSGIAEFDRVTGGGFVRGSVLLVGGDPGIGKST
                     LLIQAAAALARAGHRAVY"
     gene            complement(461..1639)
                     /gene="alr"
                     /locus_tag="VFL49_01070"
     CDS             complement(461..1639)
                     /gene="alr"
                     /locus_tag="VFL49_01070"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7910596.1"
                     /translation="MTDTALQPDLPADSGSAGHVDSSPAKADAGGILTINLAAIAANW
                     ELLSRQAMPSECAAVIKADGYGCGIEPVARALTRAGCRTFFVADISEARRVRAAAPNA
                     ALYVLSGLLPGTATTFADIRAQPVIGSLVELAEWDAFCSTNQWQGGAALHVDTGMNRL
                     GVSVHEAAALAPRIRTENHGITLLISHLACAEQPDHPLNQRQIELFREVRLLYRGIPG
                     SLANSSGIFLGNGAHCDMVRPGAALFGVNPTPGHRNPMHPVIELRARVVQVRNVPKGE
                     TIGYNATWTAKHAARIAVVAVGYADGYPRAASASDTSPGSDAIVADTRCPLAGRVSMD
                     LLAVDVTALSENTVRRGDLITLIGGPIGVDELAAAAGTIGYEVLVSLGRRYHRIYRDN
                     "
     gene            complement(1696..3195)
                     /locus_tag="VFL49_01075"
     CDS             complement(1696..3195)
                     /locus_tag="VFL49_01075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7910597.1"
                     /translation="MALADSTARKQAEEPAPLYRSAPHNIEAEQALLGAILVNNEAFY
                     RVSDFLEPVHFFEPIHQKVYQLARDLIRAGKLATPVTLKTFLDANVDIGGLTVSQYLA
                     RLAAEATTIINAEDYGHTVYDLSIRRALIQVGEDMVNVAYDAPVDFSPRNQIEDAERR
                     LYELAETGRYDGGFQRFAQALTTAIDMAAHAFQRDGKLSGVASGLDDLDRMMGGLQQS
                     DLIILAGRPGMGKTALATNIGYNIAKAWRGEVKPDGHTVTVNGGIVGFFSLEMSAEQL
                     ATRIIAEQTGIPSNQIRRGGISESDFEKIKDYSIELQNIPLYVDETGGLSVAQLAARA
                     RRLKRQRGLDLLVIDYLQLLQGSTRRSAENRVQEITEITTKLKALAKELNVPILALSQ
                     LSRQVESRDDKRPQLSDLRESGSIEQDADVVLFVYREEYYHQMRKPLESNRDKFAEWL
                     AEADKVHGKAEVIIGKQRHGPTGTVELQFEAAVTRFSSLARDTYLPERT"
     gene            complement(3354..4604)
                     /locus_tag="VFL49_01080"
     CDS             complement(3354..4604)
                     /locus_tag="VFL49_01080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET7910598.1"
                     /translation="MDRLLQFLLSRFIRRGTFKVTTSRGRTITCGDGAGRPVAVRLTT
                     RATELGILVDPELKFGEAYMDGTLVVEQGTIADVLAIALGQQAEVPNWARPQWLLRYI
                     GRRLAQFNPRKRAQRNVAHHYDLDGRLYSLFLDADRQYSCAYFETPDASLDDAQLAKK
                     RHLAAKLRLTGRQRVLDIGCGWGGLGLYLAEIGGADVTGITLSQEQHAIANERAAEKN
                     LSERARFLLQDYRDAQGPFDRIVSVGMFEHVGVGYYDAFFKKCAALLADDGVMVLHSI
                     GRSEGPGITNPWIAKYIFPGGYIPALSEVLPVVENAGLLVTDIEILRLHYAETLKAWR
                     ERFLAHREDAERIYDPQFVRMWEFYLAAAEMSFREQNMMVMQIQLTKRQGVVPITRDY
                     IFQEERRFAAAESKRRPPLRLAGE"
BASE COUNT          900 a         1429 c         1456 g          900 t
ORIGIN      
        1 atagaccgcc cggtgcccgg cgcgcgcgag cgctgcagcc gcctggatca gcaaagtcga
       61 cttcccaatg cctggatcac cgccgaccag cagcaccgaa ccgcgcacga agccaccacc
      121 ggtgacacga tcgaattcag caatcccgga aaccaggcga ggggcatcgt gcgcctcgcc
      181 ggtcagggcc tcgagcgcaa acaggcggcc cttacccggc ttccgacccg ggccggatgg
      241 gcgctcggag ccttcctcgc tgagcgtgtt ccattccccg caagcctcgc atttgccctg
      301 ccaccggctg aaagcggcac cgcagttctg gcagacgaag gttaggctgc gcttggacat
      361 aaagaaccgc cgtaaaatga ccgttcgtga ccctaaagcg accatgagaa caatacaaga
      421 acaaacttta agaagacgct aatttgggag gttttggacg tcagttatcg cggtatatcc
      481 ggtggtaccg gcggcccagg ctcaccagca cctcgtaacc gattgtccct gcggctgccg
      541 ccagctcatc caccccgatc ggcccgccga tcagggtgat gaggtcgccg cgtcgcaccg
      601 tattttcgga caatgcggtg acgtcgaccg ccagcaggtc catcgagacc cgtccggcca
      661 gtggacaacg cgtgtcggca acaatcgcat cggatccggg gctcgtgtcg gaggcgctgg
      721 ccgcgcgtgg atatccgtcg gcatagccga ccgcaacgac ggcaatgcgc gcggcatgct
      781 tggcggtcca cgtcgcattg tagccgatcg tctcgccctt cggaacgttg cggacctgca
      841 cgacgcgcgc gcgcagttca atcaccgggt gcatggggtt gcggtgaccg ggtgtcggat
      901 tgacgccgaa caaggcagcc cctgggcgga ccatgtcgca atgcgcgccg ttgccgagga
      961 agatgccgga cgaatttgcg agtgagccgg ggatgccgcg atagagcaga cggacttcgc
     1021 ggaagagttc gatctgacgc tggttgagag ggtggtccgg ctgctcggcg caagcgagat
     1081 gactgatcag caacgtaatg ccgtgatttt cggtacggat acggggcgcc agtgccgccg
     1141 cttcgtggac ggaaactccg agccggttca tgccggtatc gacatgcaac gccgccccgc
     1201 cttgccactg attggtcgag caaaacgcgt cccactccgc gagttcgacg aggctgccaa
     1261 tcaccggctg ggcgcggata tcggcgaagg ttgtcgcggt gccgggtagc aggccactga
     1321 gcacatagag cgcggcgttc ggcgccgcgg cgcgcacgcg ccgcgcctca gagatgtccg
     1381 caacaaaaaa tgtcctgcag ccggcgcggg tgagggcacg cgccacaggt tcgatcccac
     1441 agccatatcc atcggctttg atgacggcgg cacactccga cggcattgct tgccggctca
     1501 gcagttccca gtttgccgcg atggccgcga ggttgatggt caggataccg cctgcgtccg
     1561 ccttcgccgg gctggaatcg acgtgaccag ccgaaccaga atctgcgggc aagtcaggct
     1621 gcagggcggt gtcggtcata ccatttgggt ttagccgccc gcgcgcctgc agtccatgcc
     1681 ggctcagcga acgcttcagg tgcgctcggg gaggtaggtg tcacgcgcga gcgaggaaaa
     1741 tcgcgtcact gccgcctcga attgcaactc gacagttccg gtcggcccat gacgctgctt
     1801 gccgatgatg acctccgcct taccgtgcac tttatctgcc tcggccagcc attccgcgaa
     1861 cttgtcgcgg ttcgattcca ggggcttgcg catctggtgg taatattctt cgcgatagac
     1921 gaacaacacc acgtcggcgt cctgctcgat cgatccggat tcacgcaagt ccgacagttg
     1981 aggccgcttg tcgtcgcggc tttcgacctg acgcgacagt tgcgaaagcg ccaggatcgg
     2041 cacattcaat tctttcgcca gagctttgag ctttgtggtg atttcggtga tttcctgcac
     2101 gcgattttcg gctgagcggc gggtcgagcc ttggagcagc tggaggtaat cgatcaccag
     2161 caggtcgaga ccgcgctgcc gtttgagcct gcgcgcgcgg gccgcaagct gggcaacaga
     2221 aagaccgccc gtctcgtcca cgtagagtgg gatgttctgc aattcgatcg aatagtcctt
     2281 gatcttctcg aaatcgcttt cgctgatgcc accgcgcctg atctggttcg atggaattcc
     2341 ggtctgttcg gcgatgatac gggtggcaag ttgttcggcg gacatttcca acgaaaagaa
     2401 gccgacgata ccgccgttga cggtcaccgt gtggccgtcg ggcttcacct cgccgcgcca
     2461 ggccttggcg atgttgtacc cgatgtttgt tgcaagcgcc gttttaccca tgcctggccg
     2521 gccggcgagg atgatcaggt cggactgctg cagcccgccc atcatgcgat cgagatcgtc
     2581 cagccctgac gcgacacccg acaacttgcc gtcgcgctgg aacgcgtgcg cggccatatc
     2641 gatcgcggtc gtgagtgctt gcgcgaagcg ctggaaaccg ccgtcgtagc ggccggtctc
     2701 cgccagctca tatagccgcc gttcggcgtc ttcgatctgg ttgcgtggtg aaaagtcgac
     2761 cggcgcgtcg tacgcgacgt tcaccatgtc ctcgccgacc tgaatcagcg cgcggcgtat
     2821 cgagagatca tagacggtgt ggccgtagtc ttcggcgttg atgattgtcg ttgcttcagc
     2881 agcaagccgg gcaaggtact ggctgacggt gaggccccca atgtcgacgt tggcatcgag
     2941 gaacgtcttg agcgtgaccg gagtggcgag ctttccggcg cggatcaggt cgcgcgcgag
     3001 ctgatagacc ttctgatgga tcggctcaaa gaaatggacc ggctcgagaa aatctgaaac
     3061 gcggtaaaac gcctcgttgt tgaccaggat ggcgcccagc aatgcctgtt ccgcctcgat
     3121 attatgcggc gcggagcggt agagcggggc aggctcttcg gcctgcttgc gggctgtcga
     3181 atcagcgagt gccatggatg tcggtgctta cccggaaatt gtggcatagc acgcgatttg
     3241 cggccggaac gcgcaggctc tggagttgtc ccgcaattca caagccgcat cgaacggtgg
     3301 gaaagacggg tttcttcttg accgcagccg gcgatgaggg gtgagcccgc gatctattcg
     3361 ccggcaagac gcaggggcgg gcgacgcttg ctttccgcgg cggcgaaacg tcgttcttcc
     3421 tggaaaatat agtctcgcgt gattggcacg actccctgcc gcttggtcag ctggatctgc
     3481 atgaccatca tattctgctc gcggaaggac atttcggcag cagcaagata aaattcccac
     3541 atccggacaa attgcgggtc gtaaatgcgc tcagcgtctt cccggtgcgc caaaaaccgc
     3601 tcgcgccatg ctttcagcgt ttccgcatag tgcagtcgca ggatttcaat gtcggtcacg
     3661 agcaggccgg cgttctcgac cacggggaga acctccgaca gagccgggat gtagccgccg
     3721 gggaaaatat atttggcaat ccagggattg gtgatgcccg gaccttccga gcggccgatc
     3781 gaatgcagga ccatcacgcc gtcgtccgcg agcaaagcag cgcacttctt gaagaaggca
     3841 tcgtaataac caaccccgac atgctcgaac atgccgactg aaacgatgcg atcgaacggc
     3901 ccctgcgcgt cacggtagtc ctgcaagaga aagcgggcac gctcactcag atttttctcc
     3961 gcggcgcgct catttgcgat cgcgtgctgc tcttgcgata gcgtgatacc ggtgacgtcg
     4021 gcgcccccaa tttctgcgag gtaaaggcca agcccgcccc agccgcagcc aatgtccagg
     4081 acacgttgcc gtcctgtcag ccgcagcttg gcggcgagat ggcgcttctt cgcaagctgc
     4141 gcatcatcaa gcgaggcatc cggcgtttcg aagtaagcgc agctgtattg ccggtcggca
     4201 tccaaaaaga gcgagtacag acgaccgtcg aggtcgtaat gatgtgcaac gttgcgttgc
     4261 gcgcgtttgc ggggattgaa ttgagcgagg cgacgtccga tgtatcgcag cagccattgt
     4321 ggccgcgccc agttggggac ctccgcttgc tgtcccagcg caatggcgag cacatcggca
     4381 atcgtgcctt gttccacgac caacgtgcca tccatgtatg cttcgccgaa tttgagctcg
     4441 ggatcgacca gaatgccgag ttcagtagcc cgggtcgtaa gccgcaccgc gaccggcctc
     4501 ccggcgccgt cgccgcatgt aattgtccgt ccccgcgagg tcgtgacctt gaatgtcccc
     4561 cgccggataa atcgactgag caaaaactga agcaaccggt ccatcggccg cctccatcac
     4621 gcaaatccgc atgctgtcgt tggcatttgc cccgaaaggt caacgcagcg gtcccataaa
     4681 cgttc
//
LOCUS       DASSRL010000054         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1523792, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000054 DASSRL010000000
VERSION     DASSRL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523792"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFL49_01085"
     CDS             <1..494
                     /locus_tag="VFL49_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7910599.1"
                     /translation="ELVSSLQGVEAPATPISAAQLRQLAAQSLTDPSRSDRVNRAPLF
                     AQLDQLAQFTIAVQFDFNSARIRPDSFRAVGLMADALYHPYLQGYRFLIVGNTDAKGS
                     REYNLKLSQQRADAIRDALINPFGISPSRIEAVGLGEEQLLSPAKPDAAENRRVQLIN
                     IGK"
     gene            complement(646..1314)
                     /locus_tag="VFL49_01090"
     CDS             complement(646..1314)
                     /locus_tag="VFL49_01090"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7910600.1"
                     /translation="MTFRGLAVVLAGGLILGGCMQSTIEPASDANLTPRDKKLLAAAP
                     YEKATIPEPYKRHIVAYHRKEAPGTILIDSDARYLYYVLPDGKAIRYGVTVGEEALAF
                     SGVAKVGRMTEWPTWTPTEDIKKRMDVPNFVGPGPQNPMGARAMYLFEGNKDTLYRIH
                     GTNQPEYIGQAISSGCIRMTNEDVIDLYKRVKLGTVVVVLAPNQGDSPANPRVASVNP
                     FRSN"
     gene            1664..1834
                     /locus_tag="VFL49_01095"
     CDS             1664..1834
                     /locus_tag="VFL49_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910601.1"
                     /translation="MEVTKTTEQKQDSGRQYTNLDNRYGKIGISAVAAAMCHQGGQRH
                     ATEAHLDPYDRD"
     gene            2026..>2681
                     /locus_tag="VFL49_01100"
     CDS             2026..>2681
                     /locus_tag="VFL49_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET7910602.1"
                     /translation="MLSVYVPHGATLERIAVEPGSAPPDNAVWIDMVSPSVQDDKLVE
                     QMLGIAVPTREEMQEIEVSSRLYVENGARYMTATLMCQSDTPTPKTTAVTFILSSHRL
                     ATVRYDDPRPFAIVEHKLARTCPPKVSGETVLMDLLDAVIDRSADILERIGGEVDQVS
                     HSIFEPEEEVKPPSYNDVLKALGRKGDLTSKVRESLVSIGRLLLFLANEADNMKWPKD
                     "
BASE COUNT          540 a          828 c          806 g          507 t
ORIGIN      
        1 ccgagctggt tagcagcctg cagggcgtcg aagcgccggc gaccccgatc agcgcggcac
       61 aattacgtca gcttgcagcg cagtctctta ccgatccgag ccgtagcgat cgcgtcaacc
      121 gcgcgccgct gtttgcgcaa ctggaccagc tcgcgcaatt caccatagcc gttcagttcg
      181 atttcaactc tgcccgcatc cggccggatt cgttcagggc cgtcgggctc atggccgatg
      241 cgctctatca tccctatctg cagggttatc gctttctcat cgtcggcaac accgatgcga
      301 agggaagccg cgaatacaat cttaagctga gccagcagcg cgccgacgcc attcgtgatg
      361 cgttgatcaa tccgttcggc atttccccgt cgcggatcga agcagtcggc ctgggcgaag
      421 aacagctgtt gagtccggcc aagcccgacg ccgccgagaa ccgtcgtgtc cagctgatca
      481 atatcgggaa atagctggcc ggtggtccga aggcgcaccc gtgcgctcgg atagctgtca
      541 tttcgggacg cgagcgtaag cgagcggacc cggaatggcg cgcgctatag caaacagata
      601 cggcgcccaa ccgggcgccg ttttttacaa tcgatgtccg tcagctcagt tgctgcggaa
      661 tggattgacc gaggccacgc gcggatttgc cggcgagtcg ccttgatttg gcgcaagcac
      721 gacgacgacc gtgccaagct tcaccctctt gtagaggtcg atgacatcct cattggtcat
      781 gcggatgcag ccggaggaga tcgcctggcc gatatattcc ggctggttgg tgccgtggat
      841 gcggtagagc gtgtccttgt tgccttcgaa cagatacatc gcccgtgcac ccatcggatt
      901 ctgcgggccc gggccgacaa agttcggcac gtccatgcgc ttcttgatgt cttcggtcgg
      961 cgtccatgtc ggccattccg tcatgcgtcc gaccttggcg acgccggaaa aggccagcgc
     1021 ttcctcgccc acggtgacgc cgtagcggat cgccttgcca tcgggcagca cgtaatagag
     1081 ataccgcgca tccgaatcga tgaggatcgt gcccggcgct tccttgcggt gataggcgac
     1141 gatgtggcgc ttgtacggct cagggattgt cgccttttcg taaggcgcgg cggcgagcag
     1201 ctttttgtcg cgcggggtga ggtttgcgtc cgaggccggt tcgatcgtgg attgcatgca
     1261 tccaccaaga atcaggccac ccgcgaggac aaccgccaac cccctgaatg tcattgtgtc
     1321 tttccttttc cggtcgccat tgcgggcgcc cgtaccaaat tgcctcagta cgcaattaac
     1381 cgaaaagccc ccggcattgc cacgttttcg gctgtccggc gaacaccttc ctgtgaacct
     1441 gttgcaggaa tgtcgcagcg cacccaaacg gcgacctaaa caaaacgact ttggcgccaa
     1501 gaccttaagg ccgggtttac catggctgcg cctataatgc gctgcacagc gcggcagccg
     1561 caaaagtcac gagagcatac gaattgccgc gcaattccgc cccactcatg caccatcttg
     1621 tgcgcttggg gaaaaacgca aggaaggatc ggagttcagg actatggaag tcacaaaaac
     1681 aacggagcag aagcaagact ccggacgcca atacacaaac ctcgataacc gttacggcaa
     1741 gatcgggatt tcggccgttg cggcggcgat gtgccaccag ggcggacaac gccacgcgac
     1801 ggaagcccat ctcgatccct acgatcgcga ctgacgtgct gcgcacgccg gctgcttagc
     1861 cgcgcggctc ccaacttaaa accaccgcca ccaaatgtgc gcggccagag gggctgcgcg
     1921 cgaatcgaca tttatatctt gcggaaatcc gcgcgatcag gtcatgagat gtgatccgac
     1981 cacggcgtga tcgcgcaagc gaatcgcgcg caagatggtg gctgcatgct ttcggtctac
     2041 gtcccgcacg gcgcgacgct cgagcgcatt gccgtcgagc ccggctcggc gccgccggac
     2101 aacgcggtgt ggatcgatat ggtctcgccg agcgtgcagg atgacaaact cgtcgaacag
     2161 atgctcggca tcgccgtgcc gacacgcgag gagatgcagg agattgaggt ctccagccgc
     2221 ctttatgtcg agaacggcgc gcgctacatg acggcgacac tgatgtgcca gtcggacacg
     2281 ccgacgccga agacgacagc ggtcactttc attctttcca gtcatcgcct ggcgaccgtg
     2341 cgctacgacg atccgcgacc cttcgccatc gtcgagcaca agctggcgcg cacctgtccg
     2401 ccgaaagtgt ccggagaaac ggtgctgatg gatctgctcg atgccgtcat cgaccgctcc
     2461 gccgacatcc tggagcgcat cggtggcgag gtcgaccagg tgtcacattc gattttcgag
     2521 ccggaggagg aggtgaaacc gccgtcctac aacgacgtgc tcaaggccct cgggcggaag
     2581 ggcgacctga cctcgaaggt gcgcgagagc ctggtgtcga tcggccgtct gctgttgttt
     2641 cttgccaacg aagccgacaa catgaagtgg ccgaaggaca t
//
LOCUS       DASSRL010000055         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_19102, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000055 DASSRL010000000
VERSION     DASSRL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19102"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VFL49_01105"
     CDS             <1..1475
                     /locus_tag="VFL49_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7910603.1"
                     /translation="PPVTFSQERLKVEERLPAARAFIREHKLNELIDGDLGEIGIVVM
                     GGLTNSVLRALERMGLADVFGTSRVPMLVLNVVYPLVPEELRAFCAGKGAVLVVEEGY
                     PDYIEQAVNVELRRADINTRVLGKGLLPNAGEYASDVLLEGVAAFLHATRPRGIDADA
                     IAKGARDLFAHKAAAAAALGPLPPRPPAFCTGCPERPVFSAIKLMQRELGPTHISADI
                     GCHSFATFAPFSLGNSILGYGMSLASAAAVAPNMEKRPIAVMGDGGFWHNGLITGVAS
                     NLFNKGDGVLIVMQNGYASATGQQYIPSSAASKEGPAPGMGIEQTLRAMGVKWLRTVR
                     TYSVSTMVKTLKEAMKTAERGLKVIIADGECQLARQRRVRVEDAEKLKRGERVTRTRY
                     GVDDEICTGDHSCIRLSGCPSLTVKPSPDPLRTDPVATVIETCVGCGLCGEVAHAAVL
                     CPSFYRAEIVRNPTWRDRFMHRLRQKVIGWFGGYAGEAAA"
     gene            1472..3004
                     /locus_tag="VFL49_01110"
     CDS             1472..3004
                     /locus_tag="VFL49_01110"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HET7910604.1"
                     /translation="MSAPRPITILIAALGGEGGGVLTQWIVSAAAQAGFPVQSTSIPG
                     VAQRTGATTYYVEILPTKISDLNGKRPILALTPGVGDIDIAVASELLEAGRTTANGFV
                     TPDRTHVISSLSRFYAMDEKIAMGDGRFDQDKLIKVIREHAKDALLIDMDALARQSGS
                     IINAVMLGAIAGCDRLPLALEQFEAAIREDGKAVDSNLKGFRAGYDAARARLQPAKRS
                     EKKKSADATPALVEHEVARTFPAVAQATGLEGVRRLIGYQNVGYGRLYLDRLRPIIAD
                     DQAIGAQGKLLKEVARHLAVRMSYEDVVRVAQAKISPTRMQRIAREELRAKGEPFSVH
                     DFLKPGIEELCQLLPSSLALPIVRLAERKGWLGRVYFGMEINSTSISGYLRFWLLAKL
                     RVVRPYGYRYKQEQQQIEAWLQLIRDAAKHSIELASEVAECARLIKGYGDTHARGLAN
                     YRTIEARVIRPALAGHIPATRAADALASARTAALIDPDGESLAKCLAGIDGQPALRIA
                     AE"
     gene            3126..>3469
                     /locus_tag="VFL49_01115"
     CDS             3126..>3469
                     /locus_tag="VFL49_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7910605.1"
                     /translation="MAATPTGQRPTIAAEAFLRALADHGTDYLFVNPGTDFPPIVEAF
                     SRAKKTNAKVPKPILVPHENLAVGMAHGAYMMTGRPQAVMVHVNVGTANTINNLTNLS
                     RDRVPLILAAGRT"
BASE COUNT          650 a         1114 c         1111 g          594 t
ORIGIN      
        1 acccgccggt cactttctcg caggagcgcc tcaaggtcga agagcggctg ccggccgcgc
       61 gcgccttcat ccgcgagcac aagctcaacg aattgattga cggcgatctt ggcgagatcg
      121 gcatcgtggt gatgggcggg ctgaccaaca gcgtgctgcg cgcgctcgag cgcatggggc
      181 ttgccgatgt gtttggcacc tcccgtgttc cgatgctcgt cctcaacgtc gtctatccgc
      241 tggtgccgga agaactgcgc gcattctgcg cgggcaaggg cgccgtgctg gtcgttgagg
      301 aaggttatcc cgattacatc gagcaggcgg tcaacgtcga gttgcggcgg gcggacatca
      361 atacccgcgt gctcggcaag ggtttgctgc ccaacgccgg agaatatgcc tccgatgtgc
      421 tgctcgaggg ggtcgcggca tttctgcacg cgacgaggcc gcgaggcatc gatgccgacg
      481 ccatcgccaa aggcgcacgg gacctgtttg cccacaaggc agcggccgcc gccgcgcttg
      541 gtcctttgcc ccctcgcccg cccgccttct gcaccggctg tcccgagcgg ccggtcttct
      601 ccgccatcaa gctcatgcag cgcgagcttg gcccgaccca tatcagcgcc gatatcggct
      661 gccactcctt tgcgactttc gcaccgttct cgctcggcaa ttccatcctt ggctacggca
      721 tgtcgctcgc cagcgcggcg gcggtcgcac ccaacatgga aaaacgtccg atcgcggtga
      781 tgggcgacgg cggcttctgg cataacggat tgatcaccgg cgtcgcgtcc aaccttttca
      841 acaaaggcga cggcgttctg atcgtgatgc agaacggcta cgcgtcggca accggccagc
      901 agtacatccc gtcaagcgcg gccagcaagg aaggccccgc gcccggcatg ggcatcgaac
      961 agacgttgcg tgcgatgggc gtgaaatggc tgcgcaccgt gcggacctac agcgtcagca
     1021 cgatggtgaa gacgctgaaa gaagcgatga agaccgcgga gcgcggcctg aaagttatca
     1081 ttgcggacgg cgagtgccag cttgcgcgcc agcgccgcgt gcgggtggaa gatgccgaaa
     1141 agctcaagcg cggagagcgg gtcacgcgca cgcgctacgg cgtcgacgac gagatctgca
     1201 ccggcgacca ttcgtgtatt cgtctgtccg gctgcccgtc gctcacggtg aagccttcgc
     1261 ccgatccatt gcggacagat ccggtggcca ctgtcattga gacgtgcgtc ggctgtggcc
     1321 tgtgcggtga agtcgcgcac gcagcggtgc tgtgcccctc cttctatcgt gccgaaattg
     1381 ttcgcaatcc gacctggcgc gaccggttca tgcaccggtt gcgacagaag gtgatcggct
     1441 ggttcggcgg ctatgccggc gaggctgcgg catgagcgcg ccacgcccga taacgatact
     1501 gatcgccgcc ctcggcggcg aaggcggcgg cgtgctgacg caatggatcg tatcggcggc
     1561 ggcacaggcc gggttcccgg tgcagagcac gtcaattccc ggcgtcgcgc agcgaaccgg
     1621 cgccacgact tactacgtcg agattcttcc gaccaaaatc tccgatctca acggcaaacg
     1681 gccgatcctc gcgctcacgc ccggcgtcgg tgacatcgac atcgcggtgg caagcgagtt
     1741 gctggaggcg ggccgcacga ccgcaaacgg ctttgtcaca ccggatcgca cgcacgtcat
     1801 cagttcgctg agccgcttct acgcgatgga cgaaaaaatc gcgatgggcg acgggcgttt
     1861 cgaccaggac aagctcatca aggtcatcag ggagcatgcg aaggacgcgc tgttgattga
     1921 catggacgcg ctcgcaagac agagcggttc gatcatcaat gcggtgatgc tcggcgccat
     1981 tgccggatgc gatcgactgc cgctcgcgct cgagcagttt gaggccgcga ttcgcgagga
     2041 tggcaaggcg gtcgacagca atctgaaggg tttccgtgcc ggctacgacg cagcgcgtgc
     2101 ccgactgcag ccggcgaaac gttccgagaa aaagaagagc gctgacgcaa cgccggcgct
     2161 ggtcgagcac gaagtcgcac gcacctttcc cgccgttgcg caggcgactg ggctcgaagg
     2221 ggtgcggcgc cttatcggct atcagaatgt cggctatggc cggctctatc tcgatcgcct
     2281 gagaccgatc attgcagacg atcaggcgat cggcgcacaa ggcaagttac tgaaagaagt
     2341 cgcgcgtcac cttgcagttc gcatgtccta cgaggacgtc gtgcgcgttg cacaggccaa
     2401 aatttcacct acccgcatgc agcgcatcgc gcgcgaggaa ttgcgcgcga agggcgagcc
     2461 tttttccgtt cacgactttc tcaagccagg cattgaagag ctttgccagc tgctgccgtc
     2521 gtccctggcc ctccccatcg ttcggttggc ggagcgcaag ggctggcttg gccgggtcta
     2581 tttcggcatg gagatcaact cgacatcgat cagcggctac ttgcgcttct ggctgctcgc
     2641 caagctccgc gtcgtgcggc cctacggcta ccgctacaag caggagcagc agcaaatcga
     2701 ggcatggctc caactgatcc gcgacgcggc gaaacattcg atcgaattgg cgtcggaagt
     2761 cgccgaatgt gcacggctga tcaagggtta cggcgataca catgcgcgcg ggctcgccaa
     2821 ctaccgcaca atcgaagcgc gcgtgatccg cccggccttg gcgggccata tccccgcaac
     2881 gcgcgcggcc gacgccctcg ccagcgcgcg cacagcggcg ttgatcgacc ctgacggcga
     2941 aagcctggcc aagtgcctcg ccgggatcga cggccagccg gcgctgcgaa tcgccgcgga
     3001 gtaacgcgca aggctggggt gcaagcgcct ccgttgtcag tctttcctcg gtcgagtaaa
     3061 accgggcccg caaaacaaat gataatcgcg tcctcggaac cggacgcttg agggacgggg
     3121 acgctatggc cgcgacaccg acgggacaac ggccgacgat cgccgccgaa gccttcctgc
     3181 gggcgctggc cgaccacgga acggactact tgttcgtcaa cccgggcacc gacttcccgc
     3241 cgatcgtgga agcgttcagt cgcgccaaga agaccaatgc caaggtgccg aaacctattc
     3301 tggtaccaca cgagaacctt gcggtcggca tggcgcatgg cgcctacatg atgaccggcc
     3361 gcccgcaggc ggtgatggtg cacgtgaatg ttggcaccgc gaatacgatc aacaacctca
     3421 cgaacctgtc gcgcgaccgc gtcccgctga tcctggccgc cggccgcac
//
LOCUS       DASSRL010000056         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_404194, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000056 DASSRL010000000
VERSION     DASSRL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404194"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VFL49_01120"
     CDS             <1..1297
                     /locus_tag="VFL49_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7910606.1"
                     /translation="HDFRSYSSSVKMALVSGLMASGCKVCDIGLAITPMAYFAQFELN
                     VPCVAMVTASHNDNGWTGVKMGANRPLTFGPVEMKRLKEIVLGAKFDLKPGGGYEFVE
                     NFPDRYIADLTRRPKLKRKLKVVAACGNGTAGAFAPRILQAVGCDVVPLDCNLDHTFP
                     RYNPNTEDMKMLHAMRDAVLVNRADVALGFDGDGDRCGVVDNEGEEIFADKVGVMLAR
                     DMSTMHRNTTFVVDVKSTGLFLTDPVLKANGVKADYWKTGHSYIKRRVNELGAIAGFE
                     KSGHFFFNKPFGRGYDDGMIFALAVCDMLDRNPGKTMADLRRALPKTWGSPTMAPHCD
                     DEKKYAVVDSVVKQFETAKADGTKVAGQAIRELVTVNGVRVTVEDGTWGLVRASSNKP
                     ELVVVVESPVSEQRMRDMFKAVDGVLRTHPEVGAYNQTI"
     gene            1339..2493
                     /locus_tag="VFL49_01125"
     CDS             1339..2493
                     /locus_tag="VFL49_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7910607.1"
                     /translation="MRIAILGAGPAGLYLAYLIKRRQPDADVSVFEQNAADATFGFGV
                     VFSDKALDFLHEDDPQTHAEIVPHMESWNDITLVLRGDRVAIDGVGFAAIGRLDLLQL
                     LQARAASVGVRPVYRRAVQNIDDLGAADLVVGADGVNSLVRRSHANEFGANVRLLTNR
                     FAWFGTTKIFETLTQTFIETELGSFNAHHYRYAPGLSTFIVEVDEAAFARAGFAHLEN
                     AESRRLCENVFRATLDGHSLISNNSTWRQFPIVHNERWAAGNRVLIGDALHTAHFSIG
                     SGTRLAMEDAIALDKALAANADNIPAALQSFEAARRPILEKLVAGANGSARWYEQFAE
                     HMQLAPVDFAMSYITRSGRVDLDRLRKLSPRFVERFERARLGGVPPQVNN"
     gene            2540..>3244
                     /locus_tag="VFL49_01130"
     CDS             2540..>3244
                     /locus_tag="VFL49_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HET7910608.1"
                     /translation="MAYLSELDLQTYAVVVAAVLATIAYAFVRLRRRGDGALLSMVLN
                     NMTQGVVLFDAHERVLVVNDRYVEMYGLSSTVVKPGCTLAELIENRITTGSLNIDPDK
                     YRAEIMAAVREGTVMNRIVETPDGRAVLVVNRPIKSGQYWIGTHDDITERIQAERKSV
                     ALSEQERRRVVLETEIRDFRESSAAVLRTVSESTAALNSIAGALSNSSGLTSDRAASA
                     VQTTNEASANMIAAAGA"
BASE COUNT          638 a          937 c          999 g          670 t
ORIGIN      
        1 ccacgacttc cgcagctatt cttcgtctgt gaagatggcg ttggtttcgg gtctgatggc
       61 gtccggctgc aaagtctgcg atatcgggct ggcaatcacc ccgatggcct actttgcaca
      121 gttcgagctt aacgtgccgt gcgttgccat ggtcacggct tcgcacaacg acaacggctg
      181 gaccggcgtg aagatggggg cgaaccggcc gctgaccttc gggcccgtgg aaatgaaacg
      241 gctcaaggaa attgtgctcg gcgcgaaatt cgaccttaag ccgggcggcg gctacgagtt
      301 cgtcgagaac tttccggatc gctacattgc cgacctcacc aggcggccca agctcaaacg
      361 aaagctgaag gttgtcgccg cctgcggcaa cggcaccgcg ggcgcctttg caccacggat
      421 cctgcaagcg gtcggttgcg acgtcgtgcc gcttgactgc aacctcgatc acacgttccc
      481 gcgttacaat cccaacaccg aagatatgaa aatgctgcat gccatgcgtg acgcggtgct
      541 ggtcaaccgc gctgatgtgg ctctcggttt cgacggtgac ggcgaccgct gcggcgtcgt
      601 tgacaatgaa ggtgaggaaa tcttcgccga caaggtcggc gtgatgctcg cccgcgacat
      661 gtcgacaatg caccgcaaca cgaccttcgt cgtcgatgtg aaatcgaccg gtctgttcct
      721 cacggacccg gtgctgaagg ccaatggcgt gaaggccgac tattggaaga ccgggcactc
      781 ctacatcaag cggcgcgtga acgagcttgg cgctattgcc ggattcgaaa aatccgggca
      841 cttcttcttc aacaagccgt tcgggcgcgg ttacgacgac ggcatgattt ttgcgctcgc
      901 cgtttgcgac atgctggacc gtaatcccgg aaaaaccatg gctgatttgc gcagggcgct
      961 tccgaaaaca tggggctcgc cgacgatggc gccgcattgt gatgatgaga aaaaatacgc
     1021 ggtagtagac tccgttgtga agcagttcga gaccgccaag gctgacggca cgaaagttgc
     1081 cgggcaggcg atccgcgagc tggtcaccgt caatggcgtg cgcgtgacgg tcgaggacgg
     1141 cacctggggg ctggtgcggg catcgtccaa taagcctgag ctggtggtcg ttgtcgaaag
     1201 cccggtgtcc gaacagcgca tgcgcgatat gttcaaggcg gtcgacggcg tactgcgcac
     1261 gcatccggaa gtcggcgctt ataatcagac gatctgagca ccgttgccgc cagggcggat
     1321 cgtctgcgac actgagccat gcgcatcgcc atcctcggcg cgggtccggc cggcctctat
     1381 cttgcctatc tgatcaagcg gcggcagccc gatgccgacg tgagcgtgtt cgagcagaat
     1441 gccgccgacg ctactttcgg ttttggcgtc gtattctcgg acaaggccct ggactttctg
     1501 catgaggacg atccgcagac ccatgctgag attgttccgc atatggagtc gtggaacgac
     1561 atcacgctcg ttcttcgcgg cgatcgcgtg gcgatcgatg gtgtcggctt tgcggccatc
     1621 ggccggctgg acctgctgca attgttacag gcaagggcgg cctcggtcgg tgtgcgtccg
     1681 gtctatcggc gtgcagttca aaatatcgat gaccttggcg cggcagatct cgttgtcggc
     1741 gccgacgggg tcaattccct ggtgcggcgc agccacgcaa acgaattcgg ggcgaatgtt
     1801 cgactgctga caaatcgttt tgcctggttc ggtactacca agatcttcga gacgttgacg
     1861 cagacattca tcgagaccga actggggtcc tttaatgcgc accattaccg gtatgcaccg
     1921 ggactcagca ccttcattgt tgaggtcgat gaagccgcgt tcgcgcgtgc cggctttgcg
     1981 catctggaaa acgccgaaag caggcgcctt tgcgaaaacg tctttcgtgc aacgctcgac
     2041 ggccattcgc tcatttcgaa caattcgacg tggcggcaat tcccgattgt ccataacgaa
     2101 agatgggcgg cgggtaatcg tgttctgatc ggggatgcgt tgcacaccgc gcatttttcc
     2161 atcggctcgg ggaccaggct tgcgatggaa gatgcgatcg cgctcgacaa ggcgttagcc
     2221 gcgaatgcgg acaatatccc cgccgcattg caaagttttg aggcagcgcg ccggcccatc
     2281 ctcgaaaagc tggtcgccgg ggcgaacgga agcgcgcgct ggtacgagca gtttgcggag
     2341 cacatgcaac ttgcgccggt cgactttgcc atgagctata ttacccgttc cggccgggtc
     2401 gaccttgacc gcttgcgcaa gctttcgcct cgttttgtcg agcgtttcga acgggcccgg
     2461 ctcggtggcg tgccgccgca ggttaacaat taataaaagc ttgcgatcct ataaatccgc
     2521 acctcggggc ggatgagtct tggcttatct ttccgaacta gaccttcaga cgtacgctgt
     2581 cgtcgtcgct gccgttctgg caacgatcgc atacgcgttc gtccgcttgc gccgccgcgg
     2641 cgatggtgcg ctgctgtcga tggtcctcaa caatatgaca caaggcgtcg ttttgtttga
     2701 cgcgcacgag cgtgttctcg tggtcaatga tcgctacgtc gaaatgtacg gattgtcgtc
     2761 aaccgtcgtg aagcccggct gcacgctcgc ggaattgatc gaaaaccgca ttacgaccgg
     2821 aagtctcaac atcgatcccg acaaatatcg tgcggagatc atggcggccg tgcgtgaggg
     2881 cacggtgatg aatcggattg ttgagacgcc ggacgggcgc gccgttctcg tggtcaaccg
     2941 gccgatcaaa agcggtcaat actggatcgg aacacacgac gatattaccg agcggatcca
     3001 ggccgaaaga aaaagcgtcg cgctgtcgga gcaggaacgc cgccgcgtgg tgctcgaaac
     3061 cgaaatccgt gactttcgcg agagctctgc cgcggtgctg cgcaccgtct ccgaaagcac
     3121 tgccgcgctg aattcgattg ccggcgcttt gtcaaattcg tctggcctca catcggaccg
     3181 tgccgccagc gccgtgcaga cgacgaatga ggcatccgcc aacatgatcg ccgcggccgg
     3241 cgct
//
LOCUS       DASSRL010000057         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1034088, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000057 DASSRL010000000
VERSION     DASSRL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034088"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..969
                     /gene="fabA"
                     /locus_tag="VFL49_01135"
     CDS             436..969
                     /gene="fabA"
                     /locus_tag="VFL49_01135"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HET7910609.1"
                     /translation="MEPRRHSFEYEDLLACGRGELFGAGNAQLPLPPMLMFDRIAEIK
                     EEGGEHGKGLIRAELEVKPDLWFFLCHFKGDPVMPGCLGLDALWQLVGFFLGWLGAPG
                     KGRALGLGELKFSGQVLPTVKKVIYGIDIKRVMRSKLVLGIADGWLSADGNIIYRAND
                     LKVGLFKQDEALQPSGA"
     gene            1059..2276
                     /gene="fabB"
                     /locus_tag="VFL49_01140"
     CDS             1059..2276
                     /gene="fabB"
                     /locus_tag="VFL49_01140"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HET7910610.1"
                     /translation="MRRVVVTGMGIVSSIGNNTQEVLASLREAKSGIVRADKYAELGF
                     RCQVHGAPRLDPEEVVDRRAMRFLGGGAAWNHVAMEQAIRDSGLEDAEVSDERAGIVM
                     GSGGPSCRAIIEAADTARSKGPKRVGPFAVPKAMSSTASATLATWFKIKGVNYSISSA
                     CATSNHCIGNAAELIQWGKQDLVFAGGCEELDWSLSVLFDAMGAMSSKFNGTPEKASR
                     AYDKDRDGFVIAGGAGVLVLEEIERAKARGAKIYAEVAGYGATSDGYDMVAPSGEGAV
                     RCMNMALKTVKAPVDYINPHATSTPIGDIKEIEAIREVFGSKCPPISATKSLTGHSLG
                     ATGVQEAIYSLLMMQSGFICESANIENLDPAFADIPIVRERKDNVALGCVLSNSFGFG
                     GTNASVVFKRLDA"
     gene            2340..>2558
                     /locus_tag="VFL49_01145"
     CDS             2340..>2558
                     /locus_tag="VFL49_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003571243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7910611.1"
                     /translation="MPGLMEGRRGLIMGVANDHSIAWGIAKTLAQHGAKLAFTYQGDA
                     LAKRVKPLAAETGSSFLMPCDVEDIASVD"
BASE COUNT          503 a          741 c          804 g          510 t
ORIGIN      
        1 tccagcccaa cgccatgcgc tgacgtgtcg gccgcagccc gacctcacgc agcatggtct
       61 tcacgtcatg ccatgggcag cccgtgagcg tcgatcgatc cggcgaatga cccgcgtcgg
      121 ccacaacctt cgggatcact gaggttcgag ttggcgtcat tatccctcaa ttcctcacac
      181 ctgaacgatc ttcacactta aaccgccggt ccgataggcg ggccagcgaa aaccatcgta
      241 ttcccaacat atacgaaaac gcctctccag atgcaaccgt ctcgcaactg acagaaaggt
      301 cgtattgtcg cggatttgcg gaggaaatcc gtgacgctca tcaggctgtc gccatctttg
      361 cccgctaagt ttggtatgtt accaacttcg ggtatgggaa ttcagccaag cctcagtcat
      421 ggggatgcaa tctcgatgga accgcgacgg cacagcttcg agtatgaaga cctgctggcc
      481 tgcggtcgcg gcgaactttt cggtgccggc aacgcgcaac tgccgctgcc gccgatgctg
      541 atgttcgatc gtatcgcgga gatcaaggag gaaggcggcg agcacggcaa gggcctcatt
      601 cgtgccgagc tggaggtgaa gcccgatctt tggttcttcc tttgccattt caagggcgat
      661 ccggtgatgc cgggttgcct cggtctcgat gcgctgtggc agctcgtcgg atttttcctt
      721 ggctggctgg gcgctccagg taaaggcaga gcacttggtc tgggagagct gaaattttcc
      781 ggccaggtgc tgccgaccgt gaagaaagtg atctacggca tcgatatcaa gcgcgtgatg
      841 cgctcgaagc tcgtcttggg cattgccgac ggctggctgt ccgcggacgg caacatcatc
      901 taccgcgcta acgacctcaa ggtcggttta ttcaagcagg acgaagcttt gcagccaagc
      961 ggcgcctgag cctggagggc agccttgcgg aattgcctcg ggcggccaat ctgtggccca
     1021 atcataaaac tgcggccggg atcttaaggg tattcactat gcgacgtgtc gtcgtcaccg
     1081 gaatgggcat tgtctcttcc atcggcaaca atacacagga agtgctagcg agcctgcgcg
     1141 aggccaagtc cgggattgtg cgtgcggaca aatacgccga gcttggcttt cgctgccagg
     1201 tgcacggggc gccgcggctc gatccggaag aagtcgtcga tcgccgggcc atgcgctttc
     1261 tcggcggcgg tgccgcctgg aatcacgttg cgatggagca ggcaatccgc gattccggcc
     1321 ttgaggacgc ggaagtttca gacgagcgcg ccggcatcgt gatgggatcg ggcgggccgt
     1381 cgtgccgcgc gatcatcgag gctgccgata ccgcgcgcag caagggtccc aagcgcgtcg
     1441 gccccttcgc cgtgccgaaa gcgatgtcgt cgacggcatc cgcgacgctt gccacctggt
     1501 tcaagatcaa gggcgtgaac tattcgatct cgtcggcctg cgcgacgtcg aaccactgca
     1561 tcggcaacgc cgccgaactg attcaatggg gcaagcagga cctcgtattt gccggcggct
     1621 gcgaggaact ggattggtcg ctctcggtgc tgtttgacgc gatgggcgca atgtcatcga
     1681 agttcaacgg cactccggaa aaggcatcgc gcgcctacga caaggaccgt gacggcttcg
     1741 tcattgccgg cggcgccggc gtgctggtgc tggaagaaat cgagcgtgcc aaggcgcgtg
     1801 gcgccaagat ctatgcagaa gttgccggct atggcgccac ctccgatggt tacgacatgg
     1861 tggcgccgtc gggcgaaggt gccgtacgct gcatgaatat ggcgctgaag accgtgaaag
     1921 cgccggttga ctatatcaat ccgcacgcca cctctacgcc gattggcgac atcaaagaaa
     1981 tcgaggcgat ccgggaggtc ttcggttcca aatgtccgcc gatctcggcc acgaaatcgc
     2041 tcaccggcca ctctctcggc gcgactggcg tgcaggaggc catctactcg ctgctgatga
     2101 tgcaaagcgg gtttatctgc gaaagcgcca atatcgaaaa tctcgacccc gcgtttgccg
     2161 acatcccgat cgtgcgtgaa cgcaaggaca atgtcgcgct tggctgcgtg ctgtcgaatt
     2221 ctttcggatt tggcggcacc aacgcgtccg ttgtgttcaa gcggctggat gcgtaatcac
     2281 gagcagaagg aatacggccg tcgctcgtta gggggtaacg gaacaggaat tgtgttgtta
     2341 tgccggggct gatggagggc agacgcgggc tgatcatggg ggtcgccaac gaccactcga
     2401 tcgcctgggg gatcgcgaag acactcgctc agcatggcgc caagctcgcc tttacctatc
     2461 agggcgacgc actggccaag cgcgtaaagc cgctggccgc ggagacgggc tcgtcttttc
     2521 tgatgccctg cgacgtcgag gatattgcgt cggtagac
//
LOCUS       DASSRL010000058         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_701597, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000058 DASSRL010000000
VERSION     DASSRL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701597"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..532
                     /locus_tag="VFL49_01150"
     CDS             62..532
                     /locus_tag="VFL49_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7910612.1"
                     /translation="MLPPFVVYWHEPIIVRSYERMNLVCRTRILEFFSHSIILSVVIA
                     VFCTTSAFAAELVMFEQAGCVWCETFDREIAPVYPKTSEGQRAPLRRVNIDYPLPADL
                     SFVAVERLAPLFVLVDKGREIGRIRGYPGEDHFWGLLGVLIKKLDTDGTGRERG"
     gene            611..955
                     /locus_tag="VFL49_01155"
     CDS             611..955
                     /locus_tag="VFL49_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7910613.1"
                     /translation="MELHHLESRNRSSELDRMVENAKRASNFLKALAHESRLMILCIL
                     AEGEKSVSELEDLLRLRQPTVSQQLARLRADGLVSTRREGKAIYYKLASEEARIIIGA
                     IYDVFCRKLRKR"
     gene            complement(966..1757)
                     /locus_tag="VFL49_01160"
     CDS             complement(966..1757)
                     /locus_tag="VFL49_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET7910614.1"
                     /translation="MFFILSKTVAFFLLPSNFLLLLALAGVLLLATRWRRVGLRVAAA
                     GIVVLAIVGLLPVGTVLIHALESRFPPWDRARGAPDGIVVLGGGISPQLSSAFGRPVF
                     TGGGSRVLAMAKLARDYPNVRVVYSGGDPSLFGSGVPETDFVYPVLDDLGIARERVQM
                     ETRSRNTAENAAFTKMLVNPKPGERWLLVTSAVHMPRAVGCFRQAGFRVEAYPVGWET
                     APNLDLTWERRVADGLERLDIASYEWIGLLAYRLTGRTAELFPSP"
     gene            complement(1757..2452)
                     /locus_tag="VFL49_01165"
     CDS             complement(1757..2452)
                     /locus_tag="VFL49_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HET7910615.1"
                     /translation="MASAVEILRGVTTGTITTMLLKKGIRRAWMGGAMPLGFSGKRIV
                     GPAFTLRFVPVREDLATPESWSKPVSTRGAIEAMPENCVVVADAMGVTGAGIFGDILC
                     MRMVKRGVTALITDGVMRDRHGVLATGLPVWCAGVAAPASVNQLTFVGWNEPIGCGGC
                     AIFPDDVIVADDDGAVVIPKDLVDFVAHEGAEHELLESWLVQEVEKGAKLPGLYPPNE
                     ENKKRYEDWKKKR"
     gene            complement(2461..>2892)
                     /locus_tag="VFL49_01170"
     CDS             complement(2461..>2892)
                     /locus_tag="VFL49_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HET7910616.1"
                     /translation="PVAPGVMAPSGVLMVGAALVLRDLVQRRLGVEFGIGAIIAGAAI
                     SAGLAPRSLVLASATAFLLSEFADFAVYTPLARRRLVAAVIASSIAGLVVDSMVFLWL
                     AFGSLDFLTGQILGKLWMVLLAIPCVVWIRRRDERLGLKPA"
BASE COUNT          625 a          906 c          844 g          517 t
ORIGIN      
        1 ccgcgtcctg attgaattgc gttcattcga caatttgctg gacttccgct tcggctgacc
       61 tttgctgccg ccattcgtgg tatattggca cgagccgatt atcgttcgtt cgtacgagag
      121 aatgaacttg gtttgccgga cacgaattct ggaatttttt tctcacagca tcattttgtc
      181 tgtggtcatc gctgtttttt gcacaacaag cgcgtttgcg gcggaacttg tcatgttcga
      241 acaggccggc tgtgtctggt gcgagacatt cgaccgcgaa atcgcgcctg tctatcccaa
      301 aacatcagag ggccagcgcg cgcctttgcg gcgcgtaaac atcgattatc cattgcccgc
      361 ggatttgagt ttcgttgcgg tcgaacgcct tgcgccgctg ttcgtgttgg tcgacaaagg
      421 ccgcgaaata ggccgcatcc gcggctatcc gggcgaagac catttctggg gtttgctcgg
      481 cgtgctgatc aaaaaattgg acaccgacgg aacgggtaga gaacgcggtt gattgcacga
      541 aataagttgc gcgctgctga ttgatcgtct gtattgcagc gacgccgcaa caaaatttgt
      601 tggtgtacgt atggaattgc atcacctgga aagccgcaat cgttccagcg aactcgatcg
      661 catggtcgaa aacgcaaagc gcgccagcaa ctttctcaaa gcgttggcgc atgaaagccg
      721 gctgatgatt ctctgcatac tggcggaagg agaaaaatcg gtcagcgaac tcgaggatct
      781 gctccgcctg cgacagccga ccgtgtcgca gcaactcgcg cgtctgcgcg ccgatggtct
      841 ggtgtcgacg cgccgcgaag gcaaggcgat ttattacaag ctcgcgagcg aggaagcgcg
      901 tatcatcatc ggcgcaatct acgacgtgtt ctgccggaaa ctacgcaagc gctagccgct
      961 cgatattacg gcgacgggaa aagctctgcc gttcgccctg tcagacggta agcaagaaga
     1021 ccaatccact catagctggc gatatcgagc cgctccaggc catcggccac tctccgctcc
     1081 cacgtcaaat ccagattcgg tgctgtctcc cagccgacag gatatgcttc gacccgaaaa
     1141 ccggcttgcc ggaaacaacc gaccgcacgc ggcatgtgca cggcggaagt tacaagcagc
     1201 cagcgctcgc ctggctttgg attcacgagc atcttcgtaa atgcggcatt ttcagccgta
     1261 ttgcgcgacc gcgtttccat ctgcacgcgt tcgcgcgcga tgcccaggtc atcaaggacc
     1321 ggatagacga aatccgtttc aggcacgccg ctgccgaaga gacttggatc cccgcctgaa
     1381 tagacgacgc gcacattcgg ataatcgcgg gcaagcttgg ccatggcgag cacacgcgag
     1441 ccgccgccgg tgaagaccgg ccggccaaac gcgcttgaga gctgcggtga gattccgcct
     1501 ccaagcacga cgatgccatc gggcgctcca cgcgcgcggt cccacggcgg aaagcggctt
     1561 tcgagcgcgt ggatgagaac ggtcccgacc ggaagcagcc cgacgatcgc gagcacgaca
     1621 atgcctgctg cggcgacacg caggccgaca cgtcgccagc gcgtggccag cagcagcacg
     1681 cccgcgagcg ccagaagaag caaaaaattg gaaggtaaaa gaaagaaggc gacggtcttc
     1741 gagagaatga agaacatcag cgcttcttct tccaatcctc gtagcgcttc ttgttttctt
     1801 cattgggcgg ataaaggccg ggcagcttcg cccccttctc cacctcctgc accagccaac
     1861 tttcgagcag ctcgtgctcg gctccttcat gggcaacaaa atcgacgagg tcttttggga
     1921 tgaccacagc accgtcatcg tcggcgacga tcacgtcgtc gggaaatatc gcacagccgc
     1981 cgcaaccgat cggttcgttc cagccgacaa atgtcagctg gttgaccgag gccggcgccg
     2041 ccacccctgc gcaccagacc ggcaggccgg tcgccagcac gccgtgcctg tcgcgcatca
     2101 cgccgtcggt gatcagcgcg gttacgccgc gcttcaccat gcgcatgcac aggatatcgc
     2161 cgaagatgcc cgcgcctgtc acgcccattg cgtcggccac caccacgcag ttctcgggca
     2221 tcgcctcgat cgcaccgcgc gtggagaccg gcttcgacca gctctcgggt gttgcgagat
     2281 cctcgcggac cggcacgaag cgcagcgtga aggccggacc gacgatgcgc ttgccggaga
     2341 agccgagcgg catcgcaccg cccatccagg cgcggcgaat tcctttcttg agcagcatcg
     2401 tggtgatggt gccggtcgtc acaccgcgga gaatttcgac agcggacgcc atgatcatcc
     2461 tcacgcgggt ttcaggccaa gccgctcatc acgccgcctg atccagacga cgcacggaat
     2521 tgcgagcagc accatccaga gcttgccgag aatctggccg gtcaggaaat cgagcgatcc
     2581 gaacgccagc cataaaaaca ccatcgagtc gacgacgagc ccggcaatac ttgaggcgat
     2641 cacggccgcc accaggcgac ggcgcgcgag cggcgtgtaa accgcaaaat ccgcaaactc
     2701 ggacaggaga aacgcggtgg ccgaggccag cacaagcgag cgcggcgcaa ggccggcaga
     2761 aatcgcggca ccggcaatga tcgcgccgat gccaaattcg acgcccagcc gccgttgcac
     2821 cagatcgcgc aggacaagcg cggcgccgac catcagcact cccgacggcg ccatcacgcc
     2881 gggcgcaacc gg
//
LOCUS       DASSRL010000059         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1383928, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000059 DASSRL010000000
VERSION     DASSRL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383928"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1714
                     /locus_tag="VFL49_01175"
     CDS             161..1714
                     /locus_tag="VFL49_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910617.1"
                     /translation="MSQPAVTASSVRQSRAGLNGVIVIFTLLFAAAVTPILWPGLPPA
                     MVDYNNHLARMFVLARDGTAQAHPYYQVTWSLVPNLAMDLIVPRLGRHIGVETASRLF
                     YLLSQILIVTGAMAIECAVKGRLQIAGFVALIFLYSMPFAFGFENFEFGLGCALWGFA
                     CAIWLQDRAWLVRLAAHTAVVALLFTAHMFALGVYGFAVGLHELWRAWSERMPVREAL
                     VRLSALAIPSALLGVLLLAAHGTVGGSGTLWGLGYKPTWLMHILSAYSMTISEIGLLA
                     LSCLTFILARRGALRFEQSGAWLLAGFSALYVVMPFRLLDTAYTDMRVIVAAALIMPA
                     FVSVSFPSVTWARAAIAVAATLIVLIVGEVTSVWLAYREDFTAARKSFELLPKGAIVL
                     VGQTGDGADPPADLRDYPVYNVPTLAVHYADAFVPNLFTAPGKQPVAPRAPWRRLSMP
                     EGGFLPVKLLKYITERGAPAGTPQFVENWPRDFDYLYLIGSSIPNPMPDRLVFVMGAP
                     RFALYRIKK"
     gene            1822..3096
                     /gene="ccrA"
                     /locus_tag="VFL49_01180"
     CDS             1822..3096
                     /gene="ccrA"
                     /locus_tag="VFL49_01180"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HET7910618.1"
                     /translation="MPAVAKARGVAPLKDLYEIGEIPPLGHVPEKMHAWVIRKERHGP
                     PDKSFQLEVVPTWTIGDDEVLVYVMAGGVNYNGVWAGLGVPISPLDGHKHPFHIAGSD
                     ASGVVWAVGAKVKRWKVGDEVIVHCNQDDGDDEDCNGGDPLLSPSQRIWGYETPDGSF
                     AQFCRVQSRQLMPKPNHLSWEESACYTLTLATAYRMLFGHSPHTLKPGDNVLVWGASG
                     GLGVFGVQLVAASGANAIGVVSDDSKAEYVLNLGAKGVINRKDFKCWGQMPAVNSPEF
                     TQWTKEARRFGKAIWDITGKRDVDIVFEHPGEQTFPVSCLVVKRGGMVVFCAGTTGYN
                     LTFDARYVWMRQKRIQGSHFAHLKQASHANQFVLEQRIDPCMSEVFPWESIPLAHMKM
                     WQNLHPPGNMAVMVNAPRTGLRTFEDAQEAGR"
     gene            complement(3093..>3345)
                     /locus_tag="VFL49_01185"
     CDS             complement(3093..>3345)
                     /locus_tag="VFL49_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET7910619.1"
                     /translation="WLPAGRRSFGEIAPGIIATLLLWLLTGAAFGRYLANYAFAYVSM
                     YAGLASAMIALVFLYACASIFIYGGELNSVISKARVERT"
BASE COUNT          600 a         1021 c         1053 g          671 t
ORIGIN      
        1 aagcgccgcc tgcgaaagcg gtcgatgtaa cttgttggat ttcaatggct tctaaagcgc
       61 ggggaggtgg cgacagcggt gctgggcaat ccggcgcggc ggaaaggcgt tgttaaagcc
      121 gtgcgacgat gatgccgccc aagccacggg tgcagtgttc atgagccagc ctgccgtcac
      181 cgcgtccagc gtgaggcaat cccgcgccgg acttaacggc gtcatcgtca tttttacgtt
      241 gctgtttgcg gcagcggtga cgccgatcct ttggccgggc cttccgccgg cgatggtgga
      301 ttacaacaac catctcgcac ggatgttcgt gctggcacgc gacggcaccg cacaggccca
      361 tccctactac caggtcacct ggtctctcgt accgaacctt gcgatggacc tgatcgtgcc
      421 caggcttggc cgccacatcg gcgtcgagac ggcaagccgg cttttctatc tgctcagcca
      481 gatcctgatc gtgaccggcg cgatggcgat cgagtgcgcc gtcaagggac ggctccagat
      541 tgccggcttc gtcgcgctga tctttctcta cagcatgccg ttcgcgtttg gtttcgagaa
      601 tttcgaattc ggcctgggct gcgcgctctg gggatttgcc tgcgcgatct ggctgcagga
      661 ccgggcttgg ctcgtgcggc tcgccgcgca cacggcggtc gtcgcgctgc tgttcactgc
      721 gcacatgttc gcgttgggag tttatggctt cgctgtcggc ctgcatgaac tttggcgcgc
      781 ctggtcggag cggatgcctg tgcgagaggc actggtccgg ctttcggcgc ttgccattcc
      841 ttcggcgttg ctcggcgttc ttttgctggc cgcgcacggc accgtcggcg gcagcggaac
      901 gctttggggt ctcggctaca agccgacgtg gctgatgcac atcctcagcg cctacagcat
      961 gaccatttcg gagatcggct tgcttgcgct gtcctgcctg acattcatcc tcgcgcgccg
     1021 cggtgcgttg aggttcgagc aatccggcgc ctggctgctg gcgggatttt cggcgcttta
     1081 tgtggtgatg ccgttcaggc tgctcgatac agcctatacc gacatgcgtg tcatcgtcgc
     1141 cgcggcactg atcatgccgg cctttgtctc ggtatcgttc ccgagcgtga cctgggcgcg
     1201 cgctgcgatc gcagtcgccg ccaccctcat cgtccttatt gtcggcgagg tcacgagcgt
     1261 ctggctggcg taccgggagg atttcaccgc agcaaggaaa tccttcgaac ttctgccgaa
     1321 gggagcgatc gttctcgtcg ggcaaaccgg cgacggcgcg gatccgcccg ccgacctgcg
     1381 cgactatccc gtctacaacg tgccgacgct tgccgtgcac tatgcggatg cttttgtgcc
     1441 gaacctgttc acggcgccgg gcaagcaacc agtcgctccg cgtgctccct ggcgccggct
     1501 cagtatgcct gagggcgggt tcctgccggt caaattactc aaatacatca ccgagcgcgg
     1561 ggctccggca ggcacgcccc agttcgtgga gaactggccg cgcgatttcg actatctcta
     1621 tctgatcggg tcatcgatcc cgaacccgat gccggacagg ctcgtgtttg ttatgggcgc
     1681 gccccgattc gccctttacc ggatcaaaaa gtagccagcc gccgtaatcg cttgccgccg
     1741 caatgttttg cgggcgcaaa aggtattatt gcgatgcaac ataatttgcg ccagactgtt
     1801 ataggcaggg aggatcaatc gatgccggca gtcgccaaag cgcggggggt ggctccgctc
     1861 aaggacctct acgagatcgg ggaaattcca ccgctcgggc acgtgccgga gaagatgcac
     1921 gcctgggtca tccgcaagga gcggcacgga cctcccgaca aatcgtttca gctcgaggtg
     1981 gtgccgacct ggacaatcgg cgacgacgaa gtgctcgtct acgtgatggc gggtggcgtc
     2041 aactacaacg gcgtttgggc cggcctcggc gtgccgatct ctccgctcga tgggcacaag
     2101 catccgtttc acatcgccgg ctcggatgcc tccggcgtgg tgtgggctgt cggcgcgaag
     2161 gtcaagcgat ggaaagtcgg cgacgaggtc atcgtccact gcaaccagga tgacggcgac
     2221 gacgaggact gcaacggcgg cgatcccttg ctctcgccgt cgcagcgcat ctggggctac
     2281 gaaactccgg atggatcctt cgcacaattc tgccgcgtgc agtcgcgcca gctgatgccg
     2341 aagccaaacc acctctcgtg ggaagagtcg gcctgctaca ccctaacgct tgcgaccgct
     2401 tatcggatgc tgttcgggca ttctccgcac acgctcaagc ccggcgacaa cgttctggta
     2461 tggggagcct ccggcggcct cggcgtcttc ggtgttcagc tcgtggcggc atccggcgcc
     2521 aatgccatcg gcgtcgtctc cgacgactcg aaggccgaat acgttctcaa cctcggagcc
     2581 aaaggcgtta tcaaccgcaa ggatttcaag tgctgggggc agatgccggc ggtgaactcg
     2641 ccggaattca cccagtggac aaaagaggcg cgccgtttcg gcaaggccat ctgggatatt
     2701 accggaaagc gtgacgtcga catcgtcttc gagcatcccg gtgagcagac gttcccggtg
     2761 tcatgtctcg tcgtcaagcg cggcggaatg gtcgtcttct gcgccgggac gacaggatac
     2821 aatcttacgt tcgatgcgcg ttatgtgtgg atgcggcaaa aacgcattca gggatcgcat
     2881 ttcgcgcacc tcaagcaggc ttcgcacgca aaccagttcg tgctcgagca gcggatcgat
     2941 ccgtgcatga gcgaggtctt cccgtgggag agcattccgc tcgctcacat gaagatgtgg
     3001 cagaacttgc atccgcccgg caacatggcg gtgatggtga acgcgccgcg caccggcttg
     3061 aggacattcg aggacgcgca ggaagcggga cgttaggtcc gctcgacacg ggctttcgaa
     3121 atcaccgaat tcagctcgcc accgtaaatg aagatcgacg cgcaggcata aaggaagacc
     3181 agcgcgatca ttgccgatgc gagtccggcg tacatggaaa cgtaggcaaa agcataattg
     3241 gcaaggtagc ggccaaaggc ggcaccggtc agcagccaca acagcaacgt tgcgatgatt
     3301 cccggcgcga tttcgccgaa cgaacgccgg cccgccggca gccag
//
LOCUS       DASSRL010000060         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1401256, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000060 DASSRL010000000
VERSION     DASSRL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401256"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFL49_01190"
     CDS             <1..562
                     /locus_tag="VFL49_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET7910620.1"
                     /translation="AGRLSSVVSGEEAACKSIIPLMEVVAPQGVSYVGEGDLARICKI
                     AHNVMLGVVIENLIEITLLVNKMGVPRHAFLAFLNNGVMGSMFTRYKSPALVNLDWTT
                     TFTPELLRKDLDLGLELGREMDVPMPVTAATREVLQGHFGHATLQPDPQAYLNKDFAA
                     LMETMALAAGMKLTSENKNVPTGLEH"
     gene            854..1612
                     /locus_tag="VFL49_01195"
     CDS             854..1612
                     /locus_tag="VFL49_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7910621.1"
                     /translation="MGFTLYNAPQSTCSQRVRFVFNAKQLPFEEKKLNLLEGDQLTPE
                     YLKLNPNGVVPTLDHDGAIVIDSTVIIEYLDEIAPANGFTPESPVGRAQMRALMHFID
                     EMPAAAVRVPTFNLAFLPRFQKMSREDFVAMAESKPLRREFMLTMGQTGFPKHDMDAA
                     LARLRRTYERMDAEIERSGGPWLLGKKITLADVAVMPALVRMHDLGIADWQDLPRIVA
                     WFDNIRAQAAFKPTYYHGSLLSERFPHLREKAPT"
     gene            complement(1673..>2104)
                     /locus_tag="VFL49_01200"
     CDS             complement(1673..>2104)
                     /locus_tag="VFL49_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7910622.1"
                     /translation="TCYYRTFNVVRATPAQHDAYKKARDWLDNAIARIKPGASTAHIC
                     EVFPKANEFGFPDEMSAFGLQFAHGLGLNLHERPIISRVVSMDHPTEIKEGMVFVIET
                     YCPATDGFSAARIEEEVVVTDKGCRLISLFPAEELPISAKY"
BASE COUNT          428 a          610 c          633 g          433 t
ORIGIN      
        1 ggccggccgg ctgtcgtcgg tcgtctccgg cgaggaagca gcctgcaagt ctatcattcc
       61 cctgatggag gtggtcgcgc cgcagggcgt gtcctacgtc ggtgagggtg acctcgcgcg
      121 catctgcaag attgcgcaca atgtcatgct cggcgtcgtg atcgagaatt tgatcgagat
      181 cacgcttctg gtgaacaaaa tgggcgtgcc gcgacatgcc ttcctggcct ttctcaataa
      241 cggcgtgatg ggatcgatgt tcacgcgcta caagtcgccg gccctcgtca atctcgattg
      301 gacgacgact ttcactcccg aattgctgcg caaggatctc gatcttggac tcgagctcgg
      361 gcgcgagatg gacgttccga tgccggtgac ggcggccacg cgtgaggttt tgcagggtca
      421 tttcggccac gcgaccttgc aacccgaccc gcaggcctat ctcaacaagg acttcgcggc
      481 actgatggag accatggcgc tggctgccgg catgaaactc acatcagaaa acaagaacgt
      541 accgacgggc ctggagcact agcgggccgg cagtttccgg cgagctcggt acataaaaaa
      601 ggcccgttcc ggggcatcgc acatcccgct tgcgggatgt gcgcaaaaaa aagggcccct
      661 tgcggggccc tgtgtgcata aggtggagga ttcacgccgc cgtctaagcc ttagggagga
      721 aaacggcggc tgcgcacggg tccactcaat cggtccggcc ggatgaccac aagtaaaatg
      781 gccgtaaatt cctcttcagt tggttcgcgg cattatccga aagagtgagc aagcaatcaa
      841 agaggggttc ccgatgggct tcacgctgta caacgcgccg cagtcgactt gcagccaacg
      901 tgttcgtttt gtgttcaatg caaaacagct cccgttcgaa gaaaaaaagc taaatctgct
      961 cgaaggtgac caactcactc cggaatacct caagctcaat cccaatgggg tcgtgcccac
     1021 gctcgatcac gatggtgcaa ttgtcatcga ttcgacggtc atcatcgaat atctcgatga
     1081 gatcgcacca gccaatggct tcacacctga gagtcctgtg ggacgcgcgc aaatgcgcgc
     1141 tctcatgcat ttcatcgacg aaatgccggc cgcggccgtc cgggtgccga cgttcaacct
     1201 cgctttcctg ccaagattcc agaaaatgag ccgagaggac tttgtggcaa tggcggagtc
     1261 gaagccgctg cgccgcgagt tcatgctcac catgggccag accgggttcc cgaagcacga
     1321 tatggatgcg gcgctggcgc gcttgcgccg cacctatgaa cgcatggacg cggagattga
     1381 gcggagcggc gggccgtggc tgctcggcaa aaagatcacg ctcgccgacg tagcggtcat
     1441 gccggcattg gtgcggatgc acgatctcgg catcgcggat tggcaggatt taccgcgcat
     1501 cgttgcgtgg ttcgacaaca tccgcgcgca agcggcattc aagcctacgt attatcacgg
     1561 ctcactgttg agtgagcgtt ttccgcatct gcgggaaaaa gcgcccacct gagccggtgc
     1621 ttgcttattt gtgccgatcc tggcggttcg acctcgcggt cgattccctc actcaatatt
     1681 ttgcgctgat tggcaattct tccgccggga acagtgagat caggcgacag cctttatcgg
     1741 taaccaccac ctcttcctcg atgcgcgcgg ctgagaagcc gtcggtcgcc gggcaatagg
     1801 tttcgatcac gaacaccatg ccttccttga tttcggtcgg gtggtccatc gatacgacgc
     1861 gcgagatgat cggcctttcg tgcaggttca gaccgagacc gtgcgcgaac tgcaggccga
     1921 acgccgacat ttcgtccgga aagccgaatt cattcgcttt cgggaacact tcacagatat
     1981 gcgccgtgct ggcgccgggc ttgatacggg cgatggcgtt gtcgagccag tcgcgggcct
     2041 tcttgtacgc gtcgtgctgc gccggcgtcg cgcgcacaac gttgaaggtg cggtagtagc
     2101 aggt
//
LOCUS       DASSRL010000061         5335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_876570, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000061 DASSRL010000000
VERSION     DASSRL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876570"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFL49_01205"
     CDS             complement(<1..475)
                     /locus_tag="VFL49_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7910623.1"
                     /translation="MDTRPINRRRALTIIAATSASLVSGQSESHEIIDYEWRGYAMGT
                     DAHIIFSGTGREVARSAAEAAATEIERLEKALSLFRSDSEIARLNRDGILPGPTGDML
                     RAVRLACDMAFATNGLFDPTVQTLWESYVDWFSVSRDGDIPPQHAIAKAINTVDWR"
     gene            complement(465..2642)
                     /locus_tag="VFL49_01210"
     CDS             complement(465..2642)
                     /locus_tag="VFL49_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7910624.1"
                     /translation="MRRLLKRFGLRGRVIALLVTLAAIESVNLSAGATELGVAADFFA
                     RDDMPRLESIEGNPPAATIRAHDGRILGYAFSTRDVAGSIGYSGRPLDITAAVTPEGI
                     IAGARIVAHEEPILVIGIPREALAAYVADFKGFDIKAANGLRQITSARGPQAVAGASV
                     TSAVIRDAIVRSGRAVFRSRVASLGSDSGPRLDRESRRRSSWPDLVAEGAVKQLLLTR
                     GEAARLRGSTDAESDKAFIDLWLALATPSAIGETLLGQRIYESELAKIGAEDDLILIA
                     ANGLYSFRGTEWRKSGIFDRIEIQQGDKTFHLRASDHERIDVVHAAGAPEFREIGLFR
                     IARTSGFNSTEPFRLDLSLARDITSPSRLAGPAVVSLDYRIPDAYLIQPAATPSSTPG
                     REAGIRRSQQDAAQGQAALWQDIWWQRRYEIALLALMLAGLGGILIFQDWVTAHVKFY
                     RCIRIGYLLLTLAFLGFVANAQLSVVNVVTFVHALLSGFRWDLFLLDPLVFVLWSFVA
                     VSILFWGRGVFCGWLCPFGALQELLNEAARRLGVTQLQLPFGLHERLWMLKYVFFLGI
                     LAASLHSVMAAFAIAEVEPFKTAITLKFMRDWPFVIYAALLLISGLFIERLFCRYLCP
                     LGAALAIPARMRMFEWLKRYRECGAECQLCARTCTVQAIHPLGQINPNECIYCLQCQA
                     KYFDSSVCLHIKKRIERRQPRSAPPIKTPATAGASARGTADGH"
     gene            2950..4608
                     /locus_tag="VFL49_01215"
     CDS             2950..4608
                     /locus_tag="VFL49_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HET7910625.1"
                     /translation="MQQHRTTPGGQYQPSLDVLGEKPLEQPGVKPGSPVLSKAEFDQA
                     SRIYFERCAGCHGVLRKGATGKPLTPDLTTKLGFDYLRDFITYGSPGGMPNWGTSGEL
                     KPDEVELMARYLLNEPAQPPEFSLEDMKKNWKVLVPVAQRPTKKMNKLDIDNLFSVTL
                     RDSGEIALIDGGTKKIEHIIKTGYAVHISRLSASGRYLFTVGRDSKLNLIDLWMDPPQ
                     TVAELKVGTEARSVETSKFAGFEDKYAIAGTYWPPQYVLMDGATLEPLKVVATRGMTY
                     DTQEYHPEPRVASIVASHFNPEFVVNIKETGQILLVNYSDIKNLKVTAIPAERFLHDG
                     GFDKTGRYFLVAANARHRIAIVDTKDNKLVGVIDSGGQTPHPGRGANFTHPKFGPVWA
                     TSHLGNETISFIGTDPAKNKPHAWKVVQTVDGQGGGSLFIKTHPKSENLYVDTPLNPD
                     AEISSSIAVFRIKDLGGEKPKFKVLPIGQWSGISEGQRRVVQGEFNKEGTEIWFSVWN
                     NKSQESAIVVVDDKSLALKTVIRDKRLVTPTGKFNVYNTRKDIY"
     gene            4682..>5335
                     /gene="cobA"
                     /locus_tag="VFL49_01220"
     CDS             4682..>5335
                     /gene="cobA"
                     /locus_tag="VFL49_01220"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET7910626.1"
                     /translation="MTAGKVLLVGAGPGDPDLLTIKAVRAIEAADVVVYDRLISDEVL
                     RLIPKGATRICVGKRAGHHPVPQSEISRQLVALARKNRVVVRLKGGDPFIFGRGCEEA
                     IELQRAGIPFEVIPGITAAQGCAASLRVPLTHRGLATGVRYVTGHCREGEPLDLDWAS
                     LADPDTTLVIYMGLANIDEIVRQLVAHGLPGDTPALAVSQGTTRYEQKIHTPLVQLPA
                     "
BASE COUNT         1173 a         1565 c         1583 g         1014 t
ORIGIN      
        1 tccgccagtc gacggtgttg atcgccttcg cgatagcatg ttgcggtgga atatcgccgt
       61 cccgcgatac cgaaaaccaa tcgacatagc tctcccataa tgtctgcacc gtcggatcga
      121 acagtccgtt cgtcgcgaac gccatatcgc acgcgagacg cacggcacgc aacatatcgc
      181 ctgttgggcc tggcagtatt ccatcgcgat tgaggcgtgc gatttctgaa tcggacctga
      241 acagactcaa agccttttcc agccgctcga tttccgttgc cgcggcctcg gcggctgaac
      301 gcgcaacctc gcggccggtt ccgctgaaga tgatgtgcgc atcggttccc atggcgtagc
      361 cgcgccattc atagtcgata atctcatggg attcggactg cccgctgacc aggctggccg
      421 aagtcgctgc gataattgtc agcgcgcgcc gtcggtttat gggcctagtg tccatcggca
      481 gttcctctgg ccgaagcacc ggcagtagca ggtgtcttga ttggcggtgc ggatcgcggc
      541 tgccgtcgtt caatacgttt cttgatgtgc agacacacag aactgtcgaa gtatttggcc
      601 tggcactgca gacagtagat gcattcgttt ggattgatct gtccgagtgg atggatggct
      661 tgcacagtgc acgttcgcgc gcagagttgg cattcggcgc cgcattcgcg gtaccgtttc
      721 agccactcga acatgcgcat gcgggcgggg atcgcgagcg ctgcgcccag aggacaaaga
      781 taacggcaaa aaaggcgctc gataaacaat ccggagatca gaagcagcgc tgcgtaaatg
      841 acgaaaggcc aatcccgcat gaatttgagc gtgatcgcgg tcttgaacgg ctcgacttcc
      901 gctatggcga aagccgccat gaccgaatgc agggatgcag ctagaatgcc caaaaaaaat
      961 acgtatttca gcatccaaag tctttcgtgc aggccgaacg ggagctgcaa ctgggtcacg
     1021 cccagtcgac gggcagcttc attgagcaat tcctgcagcg cgccgaatgg acagagccat
     1081 ccgcagaata ctccgcggcc ccagaacagg atgctgactg caacaaagct ccacaaaacg
     1141 aagaccagcg ggtcgagcag aaacagatcc cagcggaatc cgctcagcag cgcgtggacg
     1201 aacgtgacga cgttgaccac cgacaattgc gcattggcaa cgaagccaag aaaggccagg
     1261 gtgagcagca gataaccaat tcgaatgcag cggtagaact tgacgtgtgc ggtcacccag
     1321 tcctgaaata tcaatatccc gccaagacca gcgagcatga gggcgagaag ggcaatctca
     1381 tatcttcgct gccaccagat gtcctgccaa agcgcggctt gcccttgtgc ggcatcctgc
     1441 tgcgagcgcc ggatgccggc ctcgcgcccg ggtgtcgacg aaggcgtggc ggccggctga
     1501 atcaagtaag catcaggaat gcggtaatcg agcgagacaa ccgccggtcc agcaagccga
     1561 gaaggggacg tgatatcgcg ggcgagcgaa aggtcgagcc gaaacggctc cgtcgaattg
     1621 aagcctgatg tgcgcgcgat ccgaaacagt ccgatttcac ggaattccgg tgcgcctgcg
     1681 gcatgcacga catcgatgcg ttcgtggtcg cttgcccgca agtggaacgt cttgtcacct
     1741 tgctgaatct cgatgcggtc aaatatgccg gatttgcgcc attcagtgcc ccgaaacgaa
     1801 tagagcccgt ttgcagcgat gagtatgaga tcgtcttcgg ccccaatctt ggccagttcg
     1861 ctttcataaa tccgctgtcc gagcagggtc tcgccgatgg cggaaggcgt cgccagcgcg
     1921 agccacaaat cgatgaatgc cttgtcggac tctgcgtcgg tcgatccgcg caaccgggcg
     1981 gcttctcccc gggtaagaag caattgtttc acagcgccct cggcgactaa atcgggccac
     2041 gacgagcgtc ggcgactctc ccggtcgagg cgaggaccgc tgtcggaccc aagggaggcg
     2101 actcgggagc gaaacaccgc tcggccggag cgtacgatgg cgtcacggat cacggcgctc
     2161 gtcacggatg cgcctgcgac ggcctgcggc ccgcgcgccg acgtaatttg tcgcaatccg
     2221 ttggcggcct tgatgtcgaa tcccttgaag tcggcgacat aagctgcgag agcctcgcgc
     2281 gggataccga tgacaagaat gggctcttcg tgggcaacaa ttctggcgcc ggcgatgatg
     2341 ccttcgggag tcacggctgc ggtgatgtca agcgggcgtc ccgagtagcc gatcgagccg
     2401 gcaacgtcac gggttgagaa ggcataccca agaatccggc catcgtgcgc gcgaatagtc
     2461 gcagcaggcg ggttgccctc gatgctttcg agccgtggca tgtcgtcgcg tgcaaaaaaa
     2521 tcggcggcaa cgccaagctc cgttgcgccg gcactgagat tcaccgattc tatcgcggcg
     2581 agtgtgacga gcagcgcaat aacgcgacct cgcagaccga agcgcttcaa cagccgtcgc
     2641 atgcaacgat atatgcagcg ttgcgggccg cctgaattga tttctatcaa gacgggtatt
     2701 tgatctcctc agtcaaattc cgcaacacga cggcgctcgt cacgcgtccc ttgaccgaaa
     2761 tcaatgcaag tgtggtggca atctaatcta gatgccacag ttcgactttc tatcgaacaa
     2821 tggagtgcga cgatgaatgc gggctgcagg gccggactct ttctgtcaag tttagtgagt
     2881 ggcgcaattc tcaccctaag catagtttcg gcccagcaac agcagcctgc acgtcctgcg
     2941 ccggcagatt tgcaacagca ccgcacgaca ccgggcgggc aataccaacc cagccttgac
     3001 gtgcttggcg aaaagccgct cgagcaaccg ggcgtcaagc cgggcagccc ggttctaagc
     3061 aaggccgaat tcgatcaggc aagccgtatc tattttgaac ggtgcgccgg ctgtcacggc
     3121 gttctgcgca aaggcgcaac cggcaagccg ttgacgcccg acctgaccac caagctcggc
     3181 ttcgactatt tgcgcgactt catcacctac ggttcgcccg gcggcatgcc gaactggggc
     3241 acatcgggtg aattgaagcc tgacgaagtc gagttgatgg cgcgctatct cctcaacgag
     3301 ccggcacagc caccggaatt cagcctcgag gacatgaaga aaaattggaa agtcctggtg
     3361 ccggtcgcgc aacgaccgac caagaagatg aacaagctcg atatcgacaa cctgttttcc
     3421 gttaccctgc gcgattcggg cgaaattgcg ctgatcgacg gcggcaccaa gaaaatcgag
     3481 cacatcatca agaccggcta tgccgttcac atctcgcgtc tgtccgcctc aggacgctat
     3541 ctttttacgg tcggccggga ttccaagctc aatctgatcg acctctggat ggatccgccg
     3601 cagacggttg ccgaacttaa ggtcggcacc gaagctcgct cggttgaaac gtctaaattt
     3661 gccggcttcg aggacaagta cgccatcgcc gggacctatt ggccgccgca atacgtgctg
     3721 atggacggcg ccacgctgga gccgctcaag gtcgtagcca cccgcggcat gacctacgac
     3781 acgcaagaat atcatcccga gccgcgggtc gcctcgattg tcgcctcgca tttcaatccg
     3841 gaatttgtcg tcaacatcaa ggaaaccgga cagattcttc tggtcaatta cagcgacatc
     3901 aagaatctga aggtgacggc aattcccgcg gagcggttcc tgcacgatgg cggatttgac
     3961 aagaccggtc gctatttcct ggtcgcagcg aacgcgcgcc atcgcatcgc gatagtcgat
     4021 accaaggata acaagctcgt tggcgtcatc gattcaggcg gacagacacc gcatccgggc
     4081 cgcggcgcca actttaccca cccgaaattc gggccggtct gggcgacaag tcatctcggg
     4141 aacgagacca tctcgtttat cggcaccgac ccggcgaaga ataagccgca cgcatggaaa
     4201 gttgtgcaga cggtcgacgg ccagggcggt ggatcgttgt tcatcaagac gcacccgaaa
     4261 tcggagaatc tctacgtcga cacaccgctc aatcccgatg ccgaaatttc gtcttcgatc
     4321 gcggttttca ggatcaagga cctcggcggc gagaagccaa agttcaaggt tcttccgatc
     4381 ggccaatggt cggggatctc cgaaggccag cgccgcgtcg tccagggtga attcaataag
     4441 gaaggcaccg agatctggtt ctcggtgtgg aacaacaaat cgcaggaatc cgcgatcgtc
     4501 gtcgtggacg acaagagtct ggcattaaag actgtcatcc gggacaagcg gctcgtaacg
     4561 ccgactggca agttcaacgt ctacaacaca cggaaagata tctactgagc ccgctgctgc
     4621 cgcacgtcgt ggcgtggctc gcaaggcgcg tcaattcgca tagggcgtag ggagtcacgc
     4681 catgacggcg ggaaaggttc tgttggtggg ggcgggtccg ggcgacccgg atctgctgac
     4741 catcaaggca gtgcgggcca tcgaagcggc cgatgtcgtc gtctacgacc ggttgatttc
     4801 cgatgaagtt ctccgtctta tcccgaaagg cgcgacgcgt atttgcgtcg gcaagcgcgc
     4861 cggccatcac cccgttccac aatccgagat cagccgccag cttgtggcat tggcccgcaa
     4921 gaatcgcgtc gttgttcgtc tcaagggcgg tgatcctttt attttcggcc gcggctgtga
     4981 ggaggcaatc gagctgcaac gcgccggaat tccgtttgaa gttatccccg gcattaccgc
     5041 agctcaggga tgcgctgcgt ctttgcgcgt gccgctgacg catcgcgggc tcgccaccgg
     5101 cgtgcgctat gtgaccggac attgccggga gggtgagccg ctcgatctcg actgggcgag
     5161 tctcgcagat ccggatacga cactcgtcat ctatatgggc ctggcaaata ttgacgagat
     5221 cgtgcgccag ctcgtcgcgc acgggctccc tggtgacaca ccggcgctgg cggtcagtca
     5281 aggcacgacg cgatacgaac aaaagataca cacgccgctc gtgcagttac cggcg
//
LOCUS       DASSRL010000062         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_229210, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000062 DASSRL010000000
VERSION     DASSRL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229210"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1221)
                     /gene="rfbG"
                     /locus_tag="VFL49_01225"
     CDS             complement(85..1221)
                     /gene="rfbG"
                     /locus_tag="VFL49_01225"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HET7910627.1"
                     /translation="MGLRQGTLENMAVTAARQADFWRGKRVLVTGHSGFKGSWLALAL
                     HRSGANVTGISLPPLTTPSLFTEAKIADLCRSHLSDIRDAAGVAGLIRAARPEVVFHL
                     AAQPLVRESYRDPLTTFETNVMGTVHVLEALRGLDGVRVAVMVTTDKVYRTAEIPGSH
                     REDDPLGGHDPYSASKAASEMVIESYRQSYLAEQPVAVASARAGNVIGGGDWSEDRLI
                     PDAVRAWQAGRVLRVRRPDAVRPWQHVLEPVSGYLVLAQRLWEQPSLAGAYNFGPELR
                     EAVKVRDLVELACAAYGDGAVDYGDGSEGPHESEWLVLDAGKAESLLGLSQRWTLIEG
                     VKRTMAWYRARQNGKDARALCLADMSDFEGRAMGPAPARAKASG"
     gene            complement(1182..1955)
                     /gene="rfbF"
                     /locus_tag="VFL49_01230"
     CDS             complement(1182..1955)
                     /gene="rfbF"
                     /locus_tag="VFL49_01230"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HET7910628.1"
                     /translation="MKVVILAGGLGTRISEESYLKPKPMIEIGGKPILWHIMKLYSAH
                     GANEFVICCGYKGYVIKEYFANYFLHMSDVTFDMADNSVVVHQQKAEPWRVTLVDTGE
                     NTLTGGRLKRVASYIKDESAFCFTYGDGLSNLNIRSAIEFHLSHGKLATVAAVRPPGR
                     YGALECQGPAVTAFTEKPRGDGGLINGGFFILSPRCLDLIEGDHSSWESSPIVRLAAM
                     GQMMAFEHRGFWHAMDTLRDKTMLEELWASGKAPWKIWQ"
BASE COUNT          394 a          703 c          653 g          415 t
ORIGIN      
        1 cgtggccttc agccagtccc gttgtgtcgg atcgcgacgc gggactagtg gcgttcgcag
       61 agttcgaatc gccgttctga cgtttcagcc ggacgctttt gcgcgggctg gcgcggggcc
      121 catggcacgg ccttcgaaat ccgacatgtc ggccaggcaa agcgcgcggg catccttgcc
      181 gttttgccgc gcgcgatacc aggccatcgt ccgcttgaca ccctcgataa gcgtccagcg
      241 ttgcgagagt ccgagcaggc tttcggcttt tccggcatcg agcacgagcc attcggattc
      301 atgcgggcct tcgctgccgt cgccatagtc aacagcgccg tcgccgtagg cggcgcaggc
      361 gagctcgacc aggtcgcgca ctttcactgc ctcgcggagt tccggcccaa aattataggc
      421 accggcaagc gacggctgct cccacaggcg ttgcgccagc acaagataac ccgacaccgg
      481 ctcgagcacg tgctgccatg gccgcactgc gtcgggacgg cgaacccgca gcactcttcc
      541 ggcctgccac gcgcgcacgg catcgggaat gagacggtcc tcggaccagt cgccgccacc
      601 gatgacattg ccggcgcgcg cgcttgcgac cgcgaccggc tgctcggcga gatatgactg
      661 acgatagctt tcgatcacca tttcgcttgc ggccttgctg gcgctgtacg gatcgtggcc
      721 tccgagcgga tcgtcttccc ggtgcgaacc cggtatttca gctgtgcgat aaactttgtc
      781 ggtcgttacc atcacagcga cccgcacccc atcgaggccg cgcagcgctt ccagcacatg
      841 cacggtcccc atcacgttcg tttcgaacgt ggtcagcgga tcgcgatagc tttcgcgcac
      901 cagcggctgc gccgcgagat gaaaaacgac ctcaggccgc gctgcacgta tcagaccggc
      961 gacgcccgca gcatcgcgaa tatcggacaa gtgacttcga cacaggtccg cgattttggc
     1021 ttcggtaaac agactgggcg tcgtcagcgg cggcaggctg atgccggtca cattcgcgcc
     1081 agaccggtgc agcgcgagcg cgagccatga acccttgaag ccgctatggc ccgtgacgag
     1141 gacgcgcttg ccgcgccaga aatccgcctg tcgtgcagcc gtcactgcca tattttccag
     1201 ggtgccttgc ctgaggccca cagctcttcc agcatcgtct tgtcgcgcaa cgtgtccatc
     1261 gcgtgccaga aaccgcgatg ctcgaacgcc atcatctgtc ccatggccgc gaggcgtacg
     1321 atcggtgagc tttcccagct gctgtggtcg ccttcgatca ggtcaaggca acgcggcgac
     1381 aggataaaga agccgccatt gatgagcccg ccatcgccgc gcggcttttc ggtaaaggcc
     1441 gtcaccgcag gcccttggca ctcgagtgcg ccgtagcggc ccggcgggcg caccgccgcg
     1501 accgtcgcaa gctttccgtg actgagatgg aattcgatcg ccgaacggat attcagattg
     1561 ctcagcccat cgccataagt gaagcagaag gccgactcgt ccttgatata gcttgcgacg
     1621 cgtttaaggc gtcccccggt cagcgtgttt tcgccggtgt ccaccagcgt cacgcgccaa
     1681 ggctcggcct tttgctggtg aacgacaacg ctgttgtcgg ccatatcgaa cgtcacgtcc
     1741 gacatgtgca gaaagtagtt ggcgaaatat tccttgatca cgtagccctt gtagccgcag
     1801 cagatcacga actcgttggc gccgtgcgcc gagtagagct tcatgatgtg ccagaggatc
     1861 ggcttgccgc cgatctcgat catcggcttc ggcttcaggt aactctcttc ggaaatccga
     1921 gtgccgagcc ccccggcgag gatgacgacc ttcatggacc ttgacgtgct ggttctggaa
     1981 tcgaccggag ccgacgtaac ctgcgccgtc gtcatgccgg tgagcacacg atcaactgat
     2041 tcgctatagt gcaattgtac cgcggcccgc gtaggtaggc cattccggct agcgtttccc
     2101 ggcagcggcc acgggtggcg ggaaggatcg tcgtcggttg aggacaccgt cccagaaatc
     2161 aaaat
//
LOCUS       DASSRL010000063         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_246763, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000063 DASSRL010000000
VERSION     DASSRL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246763"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      66..130
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            212..613
                     /gene="crcB"
                     /locus_tag="VFL49_01235"
     CDS             212..613
                     /gene="crcB"
                     /locus_tag="VFL49_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7910629.1"
                     /translation="MNFLTSNLMAGFAIVFVGGGIGAALRHGFNLAFARLLGTSFPYA
                     TLFENVSGSVVMGALVALFAFRTGIPHHWQLFLTTGILGGYTTFSTFSLDVALLYERG
                     EIGLAALYVLLSVLLSIGGLFGGLALVRSVA"
     gene            complement(610..1593)
                     /gene="bioB"
                     /locus_tag="VFL49_01240"
     CDS             complement(610..1593)
                     /gene="bioB"
                     /locus_tag="VFL49_01240"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET7910630.1"
                     /translation="MSLSHHTPPRAVSSLPRHDWKREEVRELFALPFPDLMFRAAQIH
                     RENFDPSEVQISTLLSIKTGGCPEDCAYCPQSAKFDTGVKAEKLMDLDAVLAEARAAK
                     AGGASRFCMGAAWRSPKDRELDKVCAMVEGVKALGMETCVTLGMLTGDQARRLKDCGL
                     DYYNHNLDTSPEYYGEIITTRTYQDRLDTLAHVRNAGIHVCCGGIIGMGEGLDDRIGM
                     IATLAGLPVHPESVPINMLVRVEGTPLAAAGTLDPLDFVRTIAVARICMPASVVRLSA
                     GREDMSEETQALCFLAGANSIFYGPKLLTTPNPGRDRDMALLDKLGMRPMD"
     gene            1725..1958
                     /locus_tag="VFL49_01245"
     CDS             1725..1958
                     /locus_tag="VFL49_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910631.1"
                     /translation="MKKLIFGAVTLAVLTAPAFAQKKDDEPLAILEREKKQQAEQVDQ
                     QYKRTMERTRRDSEAAARSDPWANMRAPNDGKR"
     gene            1968..2147
                     /locus_tag="VFL49_01250"
     CDS             1968..2147
                     /locus_tag="VFL49_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910632.1"
                     /translation="MSRDRMIRFAAVLVGAAVLFGLEQQFGVKLYLAIPAAIAAYFAT
                     LIVLSLAFGGDSQTK"
     gene            complement(2151..2606)
                     /locus_tag="VFL49_01255"
     CDS             complement(2151..2606)
                     /locus_tag="VFL49_01255"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7910633.1"
                     /translation="MSFWSNSKSSLLCFGAVVLMTGVAAAKPAYVASTVNLRAAPGTG
                     SEIVAKIPGGSLVDASACDGGWCEIAWQDKKGFAIQSALDMSGRVPQQRAAAAAPRPG
                     YRQGPGVVEGPEVVEGPVYYEAPPAVVYYGGPYYRPYYRPYWGWRRHWW"
     gene            complement(2712..>4544)
                     /locus_tag="VFL49_01260"
     CDS             complement(2712..>4544)
                     /locus_tag="VFL49_01260"
                     /inference="COORDINATES: protein motif:HMM:NF006192.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HET7910634.1"
                     /translation="CVKGSGWDMAAIEPAGLPAVRLAPLLKLAGLKALNDEDMIAIQR
                     GNLLDSAAPNPSVETLLHAFIPHKFVDHTHANAVLALCDRPDGMALCRKVYGSRAAIV
                     PYVMPGFALAVKAKEVFDAYPQCEGLILHKHGIFTFGSSACEAYDRMIDLVSRAERAL
                     QRMRRPRAFAVKLPKSVAAATDIAPVLRGLVSRPGLHFVLAHRAGDALLGYVNGRELA
                     RYSRQGPVTPDHVIRTKPRPLILSAPDAGRLDAFAAAAQKAVEKYQADYQRYFARHNA
                     RAAPKKRPLDSMPRVILAPGVGLFGVGATAKDAGIAADIAETAVSVISNAERGGRFES
                     ISEREQFEIEYWSLEQAKLAGRKQQPLQGHVVVITGGAGTIGLATAAAFRARGAEIVL
                     LDLDAPRTQLAAKPVNALGIGCDVTDGRSVTAAFDAVVAAHGGVDILVSNAGAAWQGR
                     IGDVDDATLRKSFELNFFAHQCLAQTAVQIMRRQGTGGVLLFNVSKQAINPGPDFGPY
                     GLPKAATLALMRQYAVDHGADGITSNAVNADRIRSGLLTDALIKKRAKSRGVTEQDYL
                     AGNLLGREVLAEDVAEAFVSLALARKTTGSVVTVDGGNIAAAPR"
BASE COUNT          818 a         1473 c         1415 g          838 t
ORIGIN      
        1 gggcggcaac ccggctttcc cttccggcaa aggcgttgac ccgctgcggc agcccgatta
       61 gcgttcgccg cgatggggat ggggttcccc gataaccgcc cgcgactggg ctgatgactc
      121 ctaccgcgcg acctggcggt gggactctgt ccgcaagccg ccgcagggcg gcttttttgc
      181 tgtcctgacc gacagacaaa ggacgcgact tatgaatttc ctgacctcaa atctcatggc
      241 gggcttcgcc atcgtgtttg tcggcggcgg catcggcgcc gcacttcgcc acgggtttaa
      301 tctcgccttc gcgcgcctgc tgggtacttc gtttccctac gcgaccctgt tcgagaacgt
      361 cagcgggtcc gtcgtcatgg gtgcgctcgt cgcgctgttc gcatttagaa ccggcatccc
      421 gcaccactgg cagctgttcc ttaccaccgg catcctcggc ggttacacga ctttttcgac
      481 cttttcgctc gatgtggcgc tgctgtacga gcggggtgaa atcggcctcg ccgcgctcta
      541 tgtgttgctg tcggttcttc tttcgatcgg cggcctgttt ggcggcctcg ccctcgtgcg
      601 cagcgtcgcc tagtccatcg ggcgcatgcc gagcttgtcg agcagtgcca tgtcgcggtc
      661 gcggccgggg ttcggcgtcg tgagcagctt cggcccgtag aagatggagt tggcgccggc
      721 aaggaagcag agcgcctgcg tttcctcgct catatcctcg cggcctgccg aaagccgcac
      781 gaccgacgcc ggcatgcaga tgcgcgcgac cgctattgtg cgcacgaaat cgagcggatc
      841 gagcgttccc gcggcggcca aaggcgtgcc ttccacccgc accagcatgt tgatcggcac
      901 gctttccgga tgcaccggca ggccggcaag cgtcgcgatc atgccgatac ggtcgtccag
      961 cccctcgccc attccgataa tcccgccgca gcagacatgg atgcccgcgt tgcgcacatg
     1021 cgcgagcgta tcgagcctgt cctggtacgt gcgcgtcgtg atgatctcgc cgtaatattc
     1081 cggcgaggtg tcgaggttat ggttgtagta atcgaggccg cagtccttca gccgccgcgc
     1141 ctgatctccg gtcagcatgc cgagcgtgac gcaagtctcc atcccgagcg ccttcacgcc
     1201 ctcgaccatc gcgcaaacct tgtcgagttc gcgatcctta ggcgagcgcc acgccgcgcc
     1261 catgcagaag cgtgaagcgc cgccggcctt cgccgcgcgc gcttcggcga gaacggcatc
     1321 aaggtccatc agcttctcgg ctttgacgcc ggtgtcgaat ttcgccgact gcgggcaata
     1381 ggcgcaatct tcagggcagc cgccggtctt gatcgacagc agcgtcgaga tctgtacttc
     1441 ggaggggtcg aagttctcgc ggtggatctg cgcagcccgg aacatcagat cgggaaatgg
     1501 caacgcgaac agctcacgta cctcttcgcg cttccagtcg tggcgcggca gactggaaac
     1561 cgcgcgcggc ggcgtgtgat gcgacagcga cattcctgaa tgctccggcc aaggatcggg
     1621 cagacttata gcaagatcga gcgctgcctg cgcaaggatt cctgcgattg agcgactttg
     1681 gcctctgtgg tagcttccca gaagacgcgt gggaacgttg aacgatgaaa aagctgattt
     1741 ttggcgctgt cacgcttgcg gtcctcaccg ctcctgcctt tgcgcaaaag aaggacgacg
     1801 agcctttagc gattctcgaa agggaaaaga agcagcaggc cgagcaggtt gatcagcaat
     1861 acaagcggac tatggagcgt acgcgcaggg acagcgaggc ggcggcgcgc tcggatccct
     1921 gggcaaacat gcgtgcgcca aacgacggca agcgctaggc gactcggatg agtcgcgatc
     1981 gcatgatccg tttcgcagcg gtgctggttg gtgccgcggt cctcttcggg cttgagcagc
     2041 aattcggcgt gaagctgtat ctggcgatac cggccgccat cgctgcctat tttgcgacgc
     2101 tcatcgtgct gtcgcttgcg ttcggcggcg acagccagac gaaatagccg tcaccaccag
     2161 tgtcggcgcc agccccagta cggacgatag tacgggcgat aatacgggcc gccgtaataa
     2221 acgaccgcgg gcggcgcctc gtaataaacc ggaccttcga cgacctcagg cccttcaacc
     2281 acacccggcc cttgccggta gccaggccgc ggcgcagccg ccgcagcgcg ctgttgcggc
     2341 acgcggccgc tcatgtcgag cgcgctctga atcgcaaaac ctttcttgtc ctgccaggcg
     2401 atctcgcacc agccgccgtc gcacgccgag gcatcgacca ggctgccgcc gggaatcttg
     2461 gcaacgatct cgctgccggt gccgggcgcc gcgcgcaggt tgacggttga agcaacgtag
     2521 gccggcttgg ccgcggcaac gcccgtcatg agcacgaccg cgccaaaaca taacaaagac
     2581 gatttgctgt ttgaccagaa actcatgacc ccacctccgc tccaatgcac tgcatgcaca
     2641 gcggcaggac ggtgggaacc aaaaccggcg gaatcgcggc aatgaaaaag acccgcggat
     2701 tctgcggttt gctatcgcgg ggcagcggca atgtttccac cgtcaacggt cacaaccgag
     2761 cccgtcgtct tgcgcgcgag cgcgaggctg acgaacgctt cggcgacgtc ttcggccaga
     2821 acctcacggc ccagcagatt gcctgcgaga taatcctgct cggtcacccc gcgcgacttg
     2881 gcgcgttttt tgatcagcgc gtcggtcagc agccccgagc gaatgcggtc ggcattcacg
     2941 gcatttgaag tgatgccgtc ggcaccatgg tcgaccgcat attggcgcat cagggccaag
     3001 gtcgccgctt tcggcaatcc atacggaccg aaatccgggc ccggattgat tgcctgcttg
     3061 gagacattga acagcaggac gccgccggtc ccctggcggc gcatgatttg cacggccgtc
     3121 tgcgcgagac actggtgggc aaagaaattc agctcgaaac tttttcgcaa cgtcgcgtcg
     3181 tcgacgtcgc cgatccgccc ctgccacgcc gcgccggcgt tcgagaccag aatgtccaca
     3241 ccgccatgcg cggcaacgac ggcgtcgaag gccgctgtaa ccgaccggcc gtcggtcaca
     3301 tcgcagccga taccgagcgc gttgaccggc ttggcggcga gctgcgtgcg cggcgcgtca
     3361 agatcaagta gaacaatttc ggctccgcgc gcgcgaaagg ccgcagcggt tgcaagaccg
     3421 atcgtcccgg cgccgccggt aatgacaaca acgtgtccct ggagcggttg ctgctttcgg
     3481 cccgcgagct tcgcctgctc aagcgaccaa tattcgatct cgaactgctc acgctcggaa
     3541 atgctctcga agcgcccgcc tctctcggca ttcgaaatca ccgagaccgc ggtttccgcg
     3601 atgtccgcgg caatgcctgc atcttttgcg gttgcgccga cgccaaacag gccgacaccg
     3661 ggagccagga tgacgcgcgg catcgaatcg agcggccgct ttttcggcgc agcgcgcgcg
     3721 ttgtgtcgcg caaagtagcg ctggtagtcc gcctgatatt tttccaccgc cttttgggca
     3781 gccgcggcaa aagcgtccag cctgcccgca tcgggcgcgg acaaaatcag cgggcgcggc
     3841 tttgtgcgaa tgacatgatc aggcgtcacc ggaccttgcc tgctgtagcg cgcgagctca
     3901 cggccattga cgtagccgag cagcgcgtcg cctgcgcggt gcgccagcac gaaatgcagt
     3961 ccgggtctgg agacaaggcc ccgcagcacc ggcgcgatat cggtagcagc tgccactgac
     4021 ttcggaagct tgacggcgaa ggcccgtggc cgccgcattc gttgcagagc ccgctccgcc
     4081 cgactcacca ggtcgatcat tcggtcgtag gcttcacacg cgctgctgcc gaaggtgaag
     4141 atcccgtgct tgtgcaggat caagccttcg cattgcggat aagcgtcgaa tacctctttg
     4201 gctttcacgg ccagcgcaaa gcccggcatc acatacggca cgatggcggc gcgcgatccg
     4261 tagacctttc ggcagagcgc catgccatcg ggtcggtcgc acagagcgag caccgcattg
     4321 gcatgcgtat ggtcgacaaa tttgtgcggg atgaacgcat gcagcagggt ctcgaccgac
     4381 ggattgggcg cggcgctgtc gagcaaattg ccgcgctgaa tcgcgatcat gtcctcgtcg
     4441 ttgagggcct tgaggccggc gagcttcaac agcggtgcga gccgcaccgc cggcaagccg
     4501 gccggctcga ttgccgccat atcccagccc gagcctttca cgca
//
LOCUS       DASSRL010000064        11246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_964164, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000064 DASSRL010000000
VERSION     DASSRL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964164"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFL49_01265"
     CDS             <1..571
                     /locus_tag="VFL49_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7910635.1"
                     /translation="VGKGSGLGLSMVYGLVKQSGGHVNIYSELNHGTTVKLYLPKAGT
                     ASDPVSDIRFGDHVPGGSEAILFVEDNDLVRGHVAGLLKSLGYSVVTAANGVEATELL
                     EQSRHFDLLLTDVIMPGGIDGRQLAERARILRPKLPVLFASGYSENSIVHHGRLDPGV
                     HLVNKPFRRGELAVKVRQILRTSAAGAKC"
     gene            956..1390
                     /locus_tag="VFL49_01270"
     CDS             956..1390
                     /locus_tag="VFL49_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7910636.1"
                     /translation="MANILLIDDDELVRKTLEFALGSAGHAVHLASDGRDGMKKFSTL
                     HPDLIITDIIMPHQEGIETILELRKLDALVPILAISGAGGSGHMDFLKAATALGATEV
                     LRKPFSPNALVAAVDACLRRGATRSPGAKASPPRVPNRFSDA"
     gene            1467..2987
                     /locus_tag="VFL49_01275"
     CDS             1467..2987
                     /locus_tag="VFL49_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910637.1"
                     /translation="MQRSHRRSEAKHAEIGAAISGRVTELLARERQVTAGKLHLINLE
                     GLKQRFGSQWEGKLELVANVMEGAFSRHLSPQDFFSRVGDGAYVIIFDQLNERDATLK
                     CSLIAKDVLEKLFGNIEDAKDVAIQTAAANVDGSIDLKSSNPLDTIAGLLDDAQQHAI
                     IVDAGSGAAPSFMEVSSPAPQSAPIISPIEQGPSDLSQLLRSAERRIERWQVHLPPKG
                     KVIYDPAAARTRAPQNTAVDMTPPPIVSRTRVARTSPSTRFDNAQDVRFRYQPVWYAP
                     KGVVIGYRCQLDFLTPTECISSEALVSDGGVPDAALKVDRLILHKGLTDLAQLRASGE
                     KAIVICPLHAATFAAASAWRQFEQIAYGLTAEMRSLLIFEIVDAAAVGMDRQLVNTVR
                     NLSRYARQIMARVPLHTLGFGTFHENGFSALSTSLSEFLHDEADIIRMIGRFCGGAKE
                     RGLLTLLNDASSISILVAAAAHGSDYIGGSAVAKDVSQLTGVLPFDLFDVYRPRLG"
     gene            complement(3346..4353)
                     /locus_tag="VFL49_01280"
     CDS             complement(3346..4353)
                     /locus_tag="VFL49_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910638.1"
                     /translation="MAITQWALNWDRGTLVVQSLGAMIMPRFDLPDGRSISPLATPPW
                     RSEPPTPGIPQGLQHMWGEWPCVPFGLAEPRLKVAPRWREVCHDRPDGPLHGPGSNEE
                     WQLIDRGPAHLTLRIDYPAKHPIAWLERRLSVTNGLAAVDCDLSIMPRQDVALPIAFH
                     PVVRLPSTPRSVRLRPGNFRFGLTGPYLIAPGGSVAGLDRRFTDLANITTETGRRVDL
                     SCYPLEGRFEDVLQLVGTSGRMLIDNLEERYTLEVAWDPAVFPSCLVWISNAGITTPP
                     WNSRHYAVGIEPACSAFDIGNQGSAEENPINREGERTCISFQSNQLWRTRYRLSIEAL
                     A"
     gene            complement(4369..6555)
                     /locus_tag="VFL49_01285"
     CDS             complement(4369..6555)
                     /locus_tag="VFL49_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7910639.1"
                     /translation="MPRDPRFDILFEPVDIGPKRLRNRFFQVPQCTGAGIERPGANAM
                     HREVKAEGGWAALCTEACMIHPETDQPVATVTSLFTQADVINHRHMTDSVHRWGALAG
                     VELCHAGGLSNNLGSRYVSAAVHQFPTPWIPQVYTYEAEESDLKRIVRMFGEAAKRAI
                     DAGFDIIYVHGTHGALPVQMLSPYHNRRTDGYGGDFAGRARLWIEILEVLKETAGAHC
                     AIANRFSIDQLSGPAGIEMQEDGLRFVELATRLGLVDLWDVNISSLQEWGEDAGPSRF
                     YKSNHQAPWTSQVRSIAKVPVVGVGRFTDADEMVRVVRSGQYDIIGCARPSIADPWLP
                     RKIDEGRLDDIAECIGCNQCIARFEYGVQIVCTQNPTALEEYRRGWHPERFASRAKDE
                     LILVLGAGPAGLECARVLGRRGYRVHLVEGEKALGGHLRDVVKLPGLQEWFRVVSLRE
                     TQLRRMPNVEILMGTGEVTPDDLLNYGADKVVLATGAAWIGDGTSAMGLDPVPGIDAS
                     LPLFVTPEQIFAGKAIGNSVVVLDADGYFMGVSLAEMLADWSKSVTLVTPFDRVAPYT
                     DFTLEGPNLRRMMHEKNIGHHTATWIERAEAGGRANLVLYDVNRDGYRRTTAPQKGVP
                     PRKAGVATRHLSCDTVVLCTSRRSNDAIYRSLRTRAAEWAEHSIRGVYRAGDCLAPRY
                     IADVVFDGHRMGREIDSDDPQRPRAIIREHRIWGDEAYPRLGDPVL"
     gene            complement(6594..7394)
                     /locus_tag="VFL49_01290"
     CDS             complement(6594..7394)
                     /locus_tag="VFL49_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7910640.1"
                     /translation="MARLTDKVALITGGASGIGRSGCILMAAEGAEVTVADLDLARAQ
                     SVAAEISAAGGSAQACAVDVCDAASVRAMVRSTINQFGRIDVLFHNAMSVPLVNKQDR
                     RITELPEESWHAIVGLVLTGTFYCVKYVAQRMLERKSGSIILTATVDALIGQAGLDAY
                     TAAKGGVVAMTRSMAAGLSPDGIRINTICPGFVNTPHQNVFMDNPAERRAIEQLHLMG
                     IMEPRDVAEFALFLASDESRLMTGGVHVVDSGYTAFKSNVNLDAVMQR"
     gene            complement(7411..8187)
                     /locus_tag="VFL49_01295"
     CDS             complement(7411..8187)
                     /locus_tag="VFL49_01295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7910641.1"
                     /translation="MTRLSTFAWLTLAVLFVTFMLSPLVLVVVFSFTDRTLTNFPLEG
                     LSLRWWADMLQQRQFPDAFKNSILIGLTVGVVSAIVGTMAAIGLAALPRRRSALAMGI
                     LTLPLMLPPLVLAVALLSFFVALGLHLGLQTVILGHVLFTQPFVVLVVYARMANFDYR
                     VVESARDLGASPMVAFFTVTLPIIRPIVIGAALIAVALSIDDFIIAFFTIGSGNTLPT
                     LIWGMIRTGLTPTVNAIGTLILLLTIGSTLMALRLTRYRG"
     gene            complement(8184..9020)
                     /locus_tag="VFL49_01300"
     CDS             complement(8184..9020)
                     /locus_tag="VFL49_01300"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7910642.1"
                     /translation="MEQKTTPYLLTLPSAIVFLTIFVAPFCYFFVVSFWSVDFYQLTP
                     GFSPQNYIVAVRDYAGTGVFTVVLATSAALITMALGFVYSFIARFKAGRWGQLLLLIV
                     LVTLFGGYLMKIYAWKTILGNEGLLNTFLIGVGLIDRPISALLYSPGAVVVTLVHFSL
                     PFAVLPIFASMRGIKDIEIEAAHDLGASSLDVLSTVIIPRCRNGLVTAFSFAFLITAG
                     DYVTPALVGGKQTIIGSLIAPQFGNLFNWPLGSAMSFAMLGIAIIVIVVFSRLLNWWC
                     RI"
     gene            complement(9116..10237)
                     /locus_tag="VFL49_01305"
     CDS             complement(9116..10237)
                     /locus_tag="VFL49_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7910643.1"
                     /translation="MNTTNPFPTPISRRRFTQGMGLTGVALSAGFLSRHAMAAAEVNF
                     LGWQGYDEPLLFDGFAKNNGISVNTTYIGNNDEIITKLRSGGIGQIDIATPYMGYIPV
                     LVALDLIQPIDVSRVPNLEKILPLFRNDENVNVDGKLYSVPFTWGSAPLVYDPAVVTT
                     PPSGWKDLLKPEFAGKVGMMDDPLGNVTLAGLIATDAEVPTKLTPDQLKAAIDFLIEV
                     KKQSRVVAVSWGDLADAIARGDVAATFNGWETLTKFCADKGKTVKYIYAQEGTYAWLD
                     NYCIVKDAPNLDAAYTLANRVIDAAAQKHLAENALQGIVNKDAIAALDPSVKAIYPYD
                     SIEDFGKKARFYEFPPIKADGTHATYGDWMKEYERFKTA"
     gene            complement(10317..>11246)
                     /locus_tag="VFL49_01310"
     CDS             complement(10317..>11246)
                     /locus_tag="VFL49_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018114344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910644.1"
                     /translation="SGCGKTTLLRMIGGFVLPTTGTIEIAGMDVTKLGPEHRPTNMVF
                     QGYGLFPHMNVRQNVGYGLRLQKRPKDEIAERVEQAIRLVHLTELADRAIDQLSGGQQ
                     QRVALARALVMRPKVLLLDEPLAALDLKLRHAMQDELRRIHQEIGGTFVFVTHDQGEA
                     LGLASRIAVMQAGRLEQEGSAQEIYASPATRFISTFIGEANLLSGRRTNGVVRLAAGP
                     TFPDAGPDADVSVVIRPESLALLDDNETTDMMLVGRIDDAIYLGSYVKYKVATSDSTH
                     VVVHVGSEATATVPMGELVRIGWRFGKQRVLHE"
BASE COUNT         2245 a         3437 c         3492 g         2072 t
ORIGIN      
        1 agttggcaaa ggcagcggcc tgggtcttag catggtgtac ggcttggtca agcaatccgg
       61 aggccatgtg aatatctatt cggaattgaa ccatggaacc acggtcaagc tgtacctgcc
      121 caaggcaggt acagccagcg atccggtatc cgacatccgg ttcggcgacc atgttccggg
      181 tggttcagaa gcgatcctgt ttgtggaaga caacgacctg gttcgcggcc atgtcgccgg
      241 ccttctgaag agcctgggat acagtgtcgt aaccgccgcg aacggggtgg aggcgaccga
      301 gctgttggag cagtctcggc atttcgacct gctcctgacg gacgtgatca tgccgggcgg
      361 catcgatggg cggcagctgg cagagcgggc gcggatctta cggccaaaac tgccggtgtt
      421 gttcgcgtcg ggctattccg agaactcgat cgttcatcat ggtcgcctcg atcccggtgt
      481 gcatctcgtc aacaagcctt ttcgaagggg cgagctggcc gtgaaggtgc gacagatact
      541 tcgcacgagt gcggccggcg caaagtgctg agcgcagcct ggctcgtgcg cgacaatggt
      601 ctgccccctg cgcccaacag catgcgctcg gcccttcctg cccctggccg attgccgggg
      661 agaacagaca cccctcagcc gctcctcgag cccgagcctc atgtagctgt gtgtggcagt
      721 ttgctgcgcc gcatccctcc gatttagctc aaacgccacg cgccccactt ggtggtcccg
      781 acgtcttcag ccgtttgcgc attagtgaaa aattactaat ttcgccgtac tatgatccaa
      841 aagacacact cttgtgtgca aggtgcgctg ttggattgta agcgacggat ctgaaggcac
      901 gaggccggct acggccgacg gccgagcacc acgcgagtgg cggagggaaa ctgcgatggc
      961 gaacatcttg ttgatcgacg atgacgaact tgtgcggaaa acgcttgaat tcgcgctcgg
     1021 gagtgcggga cacgccgtgc acctggcaag cgacggccgc gacgggatga agaaattctc
     1081 gacgttgcat ccagacctca tcatcaccga catcatcatg ccccatcagg aggggatcga
     1141 gacaatcctc gaacttcgaa agctggatgc cctcgtgccg atcctcgcga tcagtggcgc
     1201 tggcggttca gggcacatgg attttcttaa ggctgcgacc gcgctgggcg cgaccgaggt
     1261 gctccgaaag ccgttcagcc cgaacgcgtt ggtcgccgcc gttgacgcat gcctgcgccg
     1321 cggcgctacg cgctctcctg gcgccaaggc aagtccgccg cgggtcccca atcgattctc
     1381 cgatgcttaa gctcagccca ttgcgcaagg ctgtgaagcg gctcttgggt gacgaacccg
     1441 aaagccaggc gacgcatcag aatcaaatgc agcgatcgca ccgaaggtcg gaagcgaaac
     1501 atgccgagat cggtgcagcc atctccgggc gcgtgaccga actgctggcg cgtgagcgcc
     1561 aggttaccgc ggggaagctg catcttatca atctcgaggg gctgaagcag agattcgggt
     1621 ctcagtggga aggcaagctt gaacttgtcg cgaacgtgat ggagggcgcc ttcagccggc
     1681 atctctcgcc ccaggatttc ttcagccgcg ttggcgacgg tgcctatgtg atcatcttcg
     1741 accagctgaa cgagcgcgac gccacgctga agtgcagctt gatcgcaaag gatgtgctcg
     1801 agaagctgtt cgggaatatc gaggatgcca aggatgttgc cattcagacg gcagccgcca
     1861 acgtggacgg ctcgatcgat ttgaagtcgt caaacccgct cgacacgatt gcggggctcc
     1921 tcgacgacgc ccaacagcat gcaatcatag ttgacgcagg atcaggtgct gcaccgagtt
     1981 ttatggaagt ctcttcgcca gctccgcaga gcgctccaat tatcagtcca attgagcagg
     2041 ggccatcaga cctgtcccag ttgctcagga gcgccgagcg gcggatcgag agatggcagg
     2101 ttcatctgcc gccgaagggg aaggtcatct acgatccggc agcggcccga acgcgcgctc
     2161 ctcagaacac ggccgtggac atgacgccgc caccgatcgt ctcaaggacg cgtgtcgctc
     2221 gcacttcccc gtccacgcgc ttcgataatg cacaagacgt tcggtttcgg tatcagcctg
     2281 tctggtacgc acccaagggt gtcgtcatcg gatatcggtg ccaactggat tttttgacgc
     2341 cgacggaatg tatctcatcg gaggcattgg tctctgacgg cggcgtaccc gatgccgcgc
     2401 tcaaggtcga tcggctgatt ctccacaagg gcctgaccga cctggcgcag ctgcgggcat
     2461 ccggcgaaaa agcgatcgtg atctgccctt tgcacgccgc gaccttcgcg gccgcaagcg
     2521 cgtggcgaca gttcgagcag atcgcgtacg gtctgacggc cgaaatgcgc agtctgctta
     2581 tcttcgaaat cgttgatgct gcggcagttg gaatggatcg gcagctggtc aacacagtcc
     2641 gcaatctgtc gcgctatgcc cgacaaatca tggctcgggt gccgttgcat accctgggtt
     2701 tcggaacctt ccacgagaac ggcttcagcg ccttatctac aagtctttcc gagtttctgc
     2761 acgatgaggc ggacatcatc cggatgatcg gccggttctg cggcggagcc aaagagcggg
     2821 gattgctgac gctgctgaat gatgccagtt ctattagcat cctggttgcc gccgcagcac
     2881 acggatccga ctatattggc ggttctgctg tcgcgaagga tgtgtcccag cttaccggtg
     2941 ttttgccgtt cgatctattt gatgtctatc gaccgcggct cggctgagcc gctcagcctg
     3001 acaaccgatc gaggcaagtg ggtgcagtgg actaagcgct tacttgagac agtcgagatg
     3061 gtcggcgtct cggtggcatc aagcacggcc accggcatcg tcaagggcca tgggctcggt
     3121 tgagcagtgt gcacccgctg cgtcttcact gactccgcat agtctgtccg cgaatggttc
     3181 aaggtcaccg gcgcgctgcc caattggccg cccaagtaca aggtgtcgcc gaccaatgta
     3241 cagccagata ggcttctcca ccaggatgac cagagcgctg gctgcgacgc tgtatcgggg
     3301 gtaacaggga cggaagcgga ttgcagatat gtgcagcggc ggagtttacg ccagggcctc
     3361 gatcgatagc cgatagcgcg tgcgccacaa ttggttggat tggaatgaga tgcaggtgcg
     3421 ctcgccctcg cggttgatag ggttttcttc tgcggaccct tggttgccga tatcgaaggc
     3481 cgaacaggcc ggctcaatgc cgactgcata gtgccgactg ttccaaggcg gagtggtgat
     3541 gcccgcattg ctgatccaga ctagacagga tggaaagaca gccggatccc aggcgacctc
     3601 gagtgtgtag cgctcctcca gattgtcgat cagcatccga ccggaggtac cgacgagttg
     3661 cagcacgtct tcgaaacgac cttccagcgg atagcaagag agatcaacgc gacggccggt
     3721 ttcggtcgtg atgttggcaa gatccgtgaa gcgacgatcg agtcccgcaa ccgaaccgcc
     3781 gggcgcgatc aagtaggggc cggtgagacc gaagcggaaa ttgcccggcc gaaggcgcac
     3841 gctgcgcggc gttgacggca aacgaacgac cgggtgaaag gcgatcggca gggctacgtc
     3901 ttgccgcggc atgattgata gatcgcagtc gacggcggct aacccattcg tgacggagag
     3961 ccgccgttcc agccaggcga tcggatgctt cgcaggataa tcgatgcgca gtgtaaggtg
     4021 ggccggccct cgatcgatca gctgccattc ctcgttcgag cccggcccat ggagcggccc
     4081 gtcaggccgg tcatggcaga cctcgcgcca gcgcggcgca actttcaatc gcggctccgc
     4141 cagaccaaaa ggcacgcagg gccattcgcc ccacatatgt tgcagaccct gcgggatgcc
     4201 gggggtcggc ggctccgatc gccagggtgg cgtcgccagg ggcgaaatgc tgcggccgtc
     4261 cggcaggtcg aagcgcggca tgatcatcgc tccgagcgat tgcaccacta gagtgccgcg
     4321 gtcccaattg agtgcccatt gagtgatggc catcggaatt ccctagacct acagcacagg
     4381 atcgccgagg cgcggatacg cctcgtcgcc ccagatgcga tgttcgcgaa tgatggcgcg
     4441 gggccgctgc ggatcgtccg aatcgatctc gcggcccatg cgatggccat cgaaaacgac
     4501 atccgcaatg tagcgtggtg caaggcaatc gcccgcgcga tacacgcccc gtatgctatg
     4561 ctccgcccat tcggcggcgc gggtgcgaag tgagcggtag atggcatcgt tcgaccggcg
     4621 gctggtgcac agcacgacag tgtcacagga tagatgccgg gtcgccacac ctgccttgcg
     4681 aggcggcacg cctttctgcg gcgccgtcgt ccgccggtag ccgtcgcgat tcacgtcata
     4741 gaggaccaga ttggcgcgcc caccggcttc cgcgcgttcg atccaggtgg cggtgtggtg
     4801 gccaatattc ttttcgtgca tcatccgccg caggtttggg ccttcgagcg tgaagtcggt
     4861 atagggcgcg acgcggtcga atggcgtcac cagggtgacc gacttgctcc agtcggccag
     4921 catctcggca aggctgacac ccatgaaata tccgtcggcg tccaagacga cgacgctgtt
     4981 cccaatcgcc ttgccggcga agatctgttc cggcgtcacg aacagcggaa gacttgcgtc
     5041 gatgcccggc accggatcga ggcccatggc gctcgtgccg tcgccgatcc aggccgcgcc
     5101 agtcgccagg acgactttgt ccgcgccata gttcagcagg tcatctggag tgacctctcc
     5161 cgttcccatc aagatctcga catttggcat ccgacgaagc tgcgtctcgc gcaggctaac
     5221 gacgcgaaac cattcctgca gtccaggcag cttcacgaca tcgcgcaaat ggccgcccag
     5281 ggccttctcg ccctccacca gatgcacgcg atagccgcgc cggccgagca cgcgagcgca
     5341 ttccaatccg gcggggccgg cgccgagaac caggatcagt tcgtccttgg cgcgggaggc
     5401 gaaacgttca gggtgccagc cgcggcgata ttcctccaag gcggttgggt tctgggtgca
     5461 gacaatctgc acgccgtact cgaagcgcgc aatgcactgg ttacagccga tgcattcggc
     5521 gatgtcgtcc agccggcctt cgtcgatctt ccgcggcagc caggggtccg cgatcgaggg
     5581 gcgggcgcag ccgatgatgt cgtactggcc ggacctgacg acccgcacca tctcatcggc
     5641 gtcggtgaag cggccgacgc cgacgaccgg gaccttggcg atggagcgca cttgactggt
     5701 ccagggcgcc tgatggttcg acttgtagaa gcgggacgga ccggcatcct cgccccattc
     5761 ctgcaggctg ctgatattga catcccaaag gtcgaccagg cccagccgtg tcgccaactc
     5821 gacaaaccga aggccatcct cctgcatctc gatcccagcg ggaccggaga gctggtcgat
     5881 ggagaagcgg ttggcgatgg cgcagtgcgc gccggcggtc tcctttagaa cttccaagat
     5941 ctcgatccac agccgtgcgc ggccggcgaa gtcgccgccg taaccatcgg tgcgccgatt
     6001 gtgatagggc gacagcatct gcacgggcag tgcgccatgc gtgccatgaa catagatgat
     6061 gtcaaacccg gcatcgatcg cgcgtttcgc cgcctcgccg aacatgcgca cgatgcgctt
     6121 caggtcggat tcctcggcct cataagtgta aacctggggg atccacggcg tcggaaactg
     6181 atggactgcc gccgacacgt aacggctgcc gaggttgttc gacaagccgc cggcgtggca
     6241 aagctcgaca ccggccagtg cgccccagcg atggacggag tccgtcatgt gccggtgatt
     6301 gatcacatcg gcctgggtaa acaagctggt cacggtggca accggctgat cggtctcggg
     6361 atggatcatg caggcttcgg tgcacaacgc ggcccagccg ccctcggcct tcacctcgcg
     6421 atgcatcgcg ttggcgccag gccgctcgat ccccgcgcct gtacactgcg gaacctggaa
     6481 gaatcggttc ctcagacgct tcggcccgat gtcgaccggc tcaaacagaa tgtcaaaacg
     6541 gggatcgcgc ggcatcctct cctcacaatt ctaggcgtcg atcggcgctc gtatcagcgc
     6601 tgcatgaccg cgtccaggtt cacgttggac ttgaacgcgg tgtagccgga atcgacgaca
     6661 tggacaccgc cggtcatcaa gcgcgattca tccgaagcca ggaacagggc gaattccgca
     6721 acgtctcgcg gctccatgat gcccatcaga tgcaactgct cgatggcacg gcgttcggcc
     6781 ggattgtcca tgaagacgtt ctggtgcggc gtgttgacga aaccggggca gatcgtgttg
     6841 atccggatac cgtcgggcga caggccggcg gccatcgacc tggtcatcgc taccaccccc
     6901 cctttggctg ccgtatacgc gtcgagacct gcctgcccga tcagtgcatc gaccgtggcg
     6961 gtgagaatga tcgagcctga ctttcgctcc agcatgcgct gtgcgacata tttcacgcaa
     7021 tagaaggtgc cggtcagaac gagcccgacg atcgcatgcc agctttcctc aggcagttcg
     7081 gtgatgcgcc gatcctgctt attgaccagc ggcaccgaca tcgcattgtg gaacagtaca
     7141 tcgatccgcc cgaactggtt gatcgtagag cgcaccatcg cccgaaccga tgcggcgtca
     7201 cacacgtcga ccgcgcaggc ttgggcgctg ccgccggctg ccgaaatctc cgctgctacc
     7261 gactgcgcac gggctagatc aagatcggcc accgtgacct ctgctccttc ggccgccatg
     7321 agaatgcagc cggatcggcc gattccagag gcgccgcccg taatcagcgc aaccttgtcc
     7381 gtgagacgcg ccatatccct tcctccgtgc tcagccacga tagcgtgtca gtcgcaaggc
     7441 catcagcgtg gatccgatgg tcagcagcag gatcagcgtg ccgatcgcgt tgacggtcgg
     7501 tgtcaatccg gtgcggatca tgccccagat caatgtgggc aaggtattgc cgctgccgat
     7561 ggtgaagaag gcgatgatga aatcgtcgat cgaaagggcg acggcgatca gcgccgcgcc
     7621 gatcacaatc ggccggatga tcggtagggt caccgtgaag aaggcgacca tcgggcttgc
     7681 gcccagatca cgggcgcttt ccaccacccg atagtcgaaa ttcgccatgc gggcatagac
     7741 caccagcacg acgaaaggct gcgtgaacag gacatggccg aggatgacgg tttgcaggcc
     7801 gagatgcagg ccaagtgcga caaagaagga cagcagcgcc acggccagga cgagcggcgg
     7861 cagcatcaac ggcaaggtca ggatgcccat ggcgagcgcc gagcgccggc gcggcaaggc
     7921 ggccaggccg attgccgcca tggtaccgac gatcgcggag acgacgccga cggtcagtcc
     7981 aatcagaatg ctgttcttga acgcatcggg gaactgccgc tgctgcaaca tgtcggccca
     8041 ccagcgcagc gaaagtcctt ccagcgggaa gttggtcagc gtcctgtccg tgaaggagaa
     8101 gacaaccacc agcacgagcg gcgacaacat gaacgtcacg aacaaaacag ccagggtcaa
     8161 ccaggcgaac gtcgaaagcc gtgtcatatc ctgcaccacc aattgagcaa acggctgaac
     8221 acaacgatga caatgattgc gatccccagc atcgcaaatg acatcgccga acctagcggc
     8281 cagttgaaca ggttcccgaa ctgcggggcg atcaagctcc cgatgatggt ctgcttgccg
     8341 ccgactagcg ccggcgtcac gtagtcgccg gcggtgatca ggaatgcgaa gctgaaagcg
     8401 gtcaccaacc cattgcggca acgcgggata atgacggtgg acaacacatc cagcgacgag
     8461 gcgcccagat catgggccgc ctcgatctcg atgtccttga tgcctcgcat ggaggcgaag
     8521 atcggcagca ctgcaaaggg caagctgaaa tgcaccagcg taacaacgac ggcacctgga
     8581 ctgtagagca atgccgaaat cggccgatcg atcagcccga cgccgatcag gaaggtgttc
     8641 agcagacctt cattgcccag gatcgtcttc caggcataga tcttcatcag ataaccaccg
     8701 aacagcgtca ccagcacgat gagcagcagc agttgccccc agcggccagc cttgaaccgt
     8761 gcgatgaagg agtagacgaa gccgagcgcc atcgtgatca gcgccgcact tgtcgccagc
     8821 accacggtga acactccggt tccggcatag tcccgaacag cgacgatgta gttctgcggc
     8881 gaaaagcccg gcgtgagctg gtagaagtcg actgaccaga agctgactac gaagaaatag
     8941 cagaacggcg cgacgaagat cgtcaggaag acgatcgccg agggcagagt aagcagatag
     9001 ggagtggtct tttgctccac gcgggcctcg atctcggacg ccggtcatgg caggaaaccc
     9061 cgggccgccg gtcccgaggc gcgccattcg ccgcgagacc ggtcccggcc gtttgttacg
     9121 cagtcttgaa gcgctcgtac tccttcatcc agtcgccata ggtcgcatgg gtgccatcgg
     9181 ccttgatcgg cggaaattcg tagaagcgcg ccttcttgcc gaaatcctcg atggagtcgt
     9241 agggatagat tgccttcacg ctgggatcga gcgctgcgat ggcgtccttg ttcacgatgc
     9301 cctgcagggc attctcggcc agatgcttct gcgcagcggc gtcgatcacg cggttggcca
     9361 aggtataggc ggcatcaaga ttcggcgcat ccttgacgat gcagtaattg tcgagccagg
     9421 cgtaggtgcc ttcctgggcg tagatgtatt tcaccgtctt gcccttgtcg gcgcagaatt
     9481 tggtcagcgt ctcccagcca ttgaaggtcg ccgccacatc gccgcgagcg atcgcgtcgg
     9541 cgagatcgcc ccaactgacg gcgacgacgc gggactgctt cttgacttcg atcaggaagt
     9601 cgatcgccgc cttcagttga tccggcgtca gcttggtcgg aacctcggca tccgtggcga
     9661 tcagcccggc caaggtcacg ttgcccagcg gatcgtccat catcccgacc ttgccggcga
     9721 actccggctt cagaagatcc ttccacccgc tcggcggggt ggtgaccacg gccgggtcat
     9781 agaccagcgg cgccgaaccc caggtaaagg gcacggaata gagcttgccg tcgacattga
     9841 cgttctcgtc gttgcggaac agcggcagaa tcttttccag attgggcacg cgggaaacgt
     9901 cgatgggctg gataagatcc agcgccacca gaaccgggat atagcccata tagggcgtcg
     9961 cgatgtcgat ctgaccgata ccgcccgagc gcagcttggt gatgatctcg tcgttgttgc
    10021 cgatataggt tgtattgacg ctgatgccgt tgttcttggc gaagccgtcg aacagcagcg
    10081 gctcgtcata accctgccag ccgagaaagt tcacctcggc cgccgccatg gcgtgtcggg
    10141 aaagaaagcc cgccgagagc gcgacgccgg tcaggcccat cccctgggtg aaacgccgcc
    10201 gcgagatcgg cgttggaaac ggattggtcg tgttcattgc agttcctcct gtttcaatgt
    10261 tggttattgg tgtttcgtct cgccgccaga acacggaccg gcggcgctgc tcttgatcat
    10321 tcatggagca cgcgctgctt gccgaacctc cagccgatcc ggaccagctc gcccatcggc
    10381 acggtcgcgg tggcttcgct gccaacatgg acgacgacat gcgtgctgtc ggaggtcgcg
    10441 accttgtatt tgacgtagga tccgaggtag atggcatcgt cgatccgccc caccagcatc
    10501 atatccgtgg tctcgttgtc atccagcaac gctaggctct ccggccggat cacgaccgag
    10561 acatcggcgt cgggtccggc atccggaaac gtcggccccg cagccagccg aacgacgcca
    10621 ttcgtccgcc gaccggacag cagattggcc tcgccgatga aggtcgatat gaagcgcgtt
    10681 gccggcgatg catagatctc ctgcgcactg ccttcctgct ccagtcgacc agcctgcatg
    10741 acggcgatgc ggctggccaa gcccagtgct tcgccctggt cgtgggtcac gaagacgaag
    10801 gttccgccga tctcctgatg gatgcggcgc agctcgtcct gcatcgcatg gcgcagtttc
    10861 aagtccagcg ccgccaacgg ctcgtcgagc agcagcactt tcggccgcat cacgagcgcg
    10921 cgggcgagcg cgacgcgctg ctgctggccc ccggagagct ggtcgatggc acggtccgcg
    10981 agttccgtca gatgtaccag acggatcgcc tgctcgacac gttcggcgat ctcgtccttt
    11041 ggacgcttct gcagccgcag gccatagccc acattctggc ggacattcat gtggggaaac
    11101 aagccgtacc cctgaaatac catattggtc ggccgatgtt cggggccgag cttcgtgaca
    11161 tccatcccag caatttcgat tgttccggta gttggcagaa cgaagccgcc gatcatgcgc
    11221 agcaaggtgg tcttgccgca gccgga
//
LOCUS       DASSRL010000065         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_316667, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000065 DASSRL010000000
VERSION     DASSRL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316667"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VFL49_01315"
     CDS             complement(<1..1125)
                     /locus_tag="VFL49_01315"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HET7910645.1"
                     /translation="MQREQRTRRERRAVGGVKQLPWRTYRNPYKPIEVLNEEQIERIH
                     DASMRILEEFGIEFLDAQARERAIAKGAELVPDTQRLRFDRNLIAEYVGRAPSEFILH
                     ARVPERNLTFGGNHINFGSVASAPNVSDLDNGRRPGNFTDYCNLLRLVQSLNVVQFIG
                     GYPVEPADIPPSTRHLDAHYAAITLTDRIWHPYSLGRERILDAVEMIAIARGISKEQL
                     AREPSLFSIVNSNSPLRLDVPMLQGLMAMAEHGQPVVLTPFTLSGAMAPVTIAGALAL
                     QNAEALAGLCFIQMINPGVPCVYGGFTSNVDMKSGAPAFGTPEYTRAAMAGGQLARHY
                     KLPYRSSNACAANAVDAQAAYESEMSLWGAVMGHANLVLHS"
     gene            complement(1307..2203)
                     /locus_tag="VFL49_01320"
     CDS             complement(1307..2203)
                     /locus_tag="VFL49_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910646.1"
                     /translation="MRIITRAAMYLLAAGLWAATVLMTGNAAMAAADAEVTISATNGI
                     DGKAISEPVSWTITKLDKNGKPSKTPAVQQAAAVLKTKLKPGQYQVLGQAKGMRAKQT
                     FTVGKEPVARTLTFGMSDISIKMIMSKGRKPVTESIQWELLTYVKGQPDKGKHVDMVT
                     APTAHFSVVAGGYVVRATYKGITADLVVPLRAGQAYDYTLNLYAGYADAAAFNGSKKV
                     MQKVTWQVVRQKPNEKGEYDLVTEVTSTEPKIMLREGKYLLIARFGDMWGKENLNVTA
                     SEVAKIKVKLVKDTNVPVVASK"
BASE COUNT          372 a          750 c          775 g          468 t
ORIGIN      
        1 gctgtgcagc accaggttgg cgtggcccat gaccgcgccc cacagtgaca tttcggattc
       61 atacgccgcc tgcgcatcga ccgcgttggc cgcacaggcg ttgctggaac ggtacggcag
      121 cttgtaatgc cgcgcgagct ggccgcccgc catggcggcg cgggtgtatt ccggcgtacc
      181 gaaggcgggc gcgccgctct tcatgtcgac gttggaggtg aacccgccat agacgcaggg
      241 cacgccggga ttgatcatct ggatgaagca gaggccggcc agcgcctcgg cgttctgcag
      301 cgcgagcgcg ccggcgatgg tcaccggcgc catcgcgccc gacagcgtga agggcgtcag
      361 caccacgggc tggccgtgtt ccgccatcgc catcaagcct tgcagcatcg ggacgtcgag
      421 gcgcagcggc gagttgctgt tgacgatcga gaacaggctc ggctcgcgcg ccagctgctc
      481 cttgctgatg ccgcgcgcga tcgcgatcat ctccacggcg tcgaggatgc gctcacgccc
      541 caggctgtag ggatgccaga tgcggtcggt gagggtgatc gcggcgtagt gcgcatcgag
      601 gtggcgcgtg gacgggggaa tgtcggccgg ctccaccgga tagccgccga tgaactgcac
      661 gacattgagg ctctgcacca ggcggagcag gttgcagtag tcggtgaagt tgcccgggcg
      721 tcgcccgttg tcgaggtctg agacgttcgg cgccgacgcc accgaaccga aattgatatg
      781 gtttcccccg aacgtcaagt tgcgctcggg cacgcgcgca tggaggatga attcggacgg
      841 tgccctgccg acatactcgg cgatgaggtt gcggtcgaag cggaggcgct gcgtgtcggg
      901 caccagctcg gcgcccttgg cgatcgcgcg ctcgcgcgcc tgcgcgtcca ggaactcgat
      961 gccgaactcc tccagtatcc gcatcgaggc atcgtggatg cgctcgatct gctcctcgtt
     1021 cagcacctcg atcggcttgt aggggttgcg ataggttcgc catggcaact gcttcacgcc
     1081 gccgacggcg cggcgttcgc ggcgcgtgcg ctgctctcgt tgcatggacg atcctttcgg
     1141 cccggacggc ttcgctcaat cgatccgata ccagccctcc ccgcctcctg ccaatgagat
     1201 ttgggccgcg cgccggcacg atttttcgat ggtcggttca gcttcgtgcg aaatgaaacg
     1261 ggggccggct ggcccccgcg caaggattgc gacgctcgcg gccccgctac ttggaggcga
     1321 cgaccgggac gttggtgtcc ttgaccagct tgaccttgat tttggcgact tcgctggcgg
     1381 tgacgttgag gttctccttg ccccacatgt cgccgaagcg ggcgatcagc agatacttgc
     1441 cctcccgcag catgatcttg ggctcggtag aggtgacttc agtcaccagg tcatattcgc
     1501 ccttctcgtt cggcttctgg cgcaccactt gccacgtcac tttctgcatg acctttttgc
     1561 tgccgttgaa ggctgccgca tcggcatagc cggcataaag gttgagcgtg tagtcataag
     1621 cctgcccggc acgtagcggt accacgaggt cggcggtgat gcccttgtag gtggcgcgca
     1681 cgacatagcc gccggcaacg accgagaaat gggcggtcgg cgcggtcacc atgtcgacgt
     1741 gcttgccctt gtccggctgg cccttcacgt aggtcaggag ctcccactga atcgactccg
     1801 tcacaggctt gcggcccttg gacatgatca tcttgatgct gatgtcggac atgccgaacg
     1861 tcagcgtccg cgccaccggc tccttgccga ccgtgaaggt ctgcttggcg cgcatgccct
     1921 tcgcctggcc gagaacctgg tactgaccgg gcttcagctt ggttttcagg accgccgcgg
     1981 cctgctgcac ggccggggtc ttcgaaggct tgccgttctt gtcgagcttg gtgatggtcc
     2041 aggagaccgg ctcgctgatc gctttgccgt cgatcccgtt ggtggcgctg atggttacct
     2101 cggcgtcggc ggccgccatc gcggcgttgc cggtcatcag tacggtcgcc gcccacaggc
     2161 cggcagccag aagatacatg gcagcgcgcg tgatgatccg cacggggcac ccctcgtaaa
     2221 gccaaatatg tgaacgagtt aataccgtag ggactcgtct gctctcaagc gttccgtcgc
     2281 cttgcatcat ccggcgactt tcttaagagt tcctttccag ggccctcagc ctggcgccaa
     2341 tgaaggcagc catatggaag cgcgc
//
LOCUS       DASSRL010000066         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_754349, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000066 DASSRL010000000
VERSION     DASSRL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754349"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFL49_01325"
     CDS             complement(<1..980)
                     /locus_tag="VFL49_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET7910647.1"
                     /translation="MTANTAFHIAVLPGDGIGPEVMAPSLEILRRIEATTPSLKFRFT
                     EGAAGAGHYKETGKSMPDSTIKLCTEADAILLGACGLPSVRYPDNTEIMPQVELRFIF
                     DLYAGVRPARLIPGVPSPIVDAHRRGIDFILIRESTEGLFASMGKGIVNQDEARETLV
                     ITRKTSQRLFDFSLRLAERRKKRGKPGLLTCVDKANAFKAYAYFRSIFDERAKAFPNV
                     KTDRIYVDACAALMVRKPWDFDVMVMENIFGDILSDLAAGLIGGLGIAPSADIGDKHA
                     VFQPCHGTAPDIMGQGKANPTAMILSAAMMLDWLADKHDHSAAADAAVRI"
     gene            1066..1851
                     /locus_tag="VFL49_01330"
     CDS             1066..1851
                     /locus_tag="VFL49_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7910648.1"
                     /translation="MPRFAANLGYLFTERPLLERIDAAAAAGFKAIELQFPYDVPAGA
                     VKAAIIKNNLTILGINTPPGEREGEFGLAAVPGREKDWQVLFARALDYASAIGGGAIH
                     CLAGKIAPEQRPAADRVFIDNLVRAADLAATKNITLLIEPINARDRPNYFLNHVEHAA
                     DVIAKTGKPNVRMQFDFYHVQIVGGDLIHRLEKFLPVIGHLQCAAVPSRHEPDEGEVN
                     YPAIFEAVDRIGYGGWIGAEYRPRARTEAGLGWARGYGVVATH"
     gene            1932..2492
                     /locus_tag="VFL49_01335"
     CDS             1932..2492
                     /locus_tag="VFL49_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910649.1"
                     /translation="MWGRKKDQVKSADSPQPLNDAVREARIEAAERSGVVVDLRDADV
                     ARLDLLNDALGPLFDEVPNDVDLFDRGVSKGDTPRLWIDVVAHVEMGRDKRQYRFVQD
                     TRYGRAVLAESYEVPAMADAIKRYVARRLVERERALADDAGSGVAQAVLYRGRRRRRL
                     RAVGMFIFGVFVGIAGLIALALLTRP"
BASE COUNT          473 a          776 c          813 g          515 t
ORIGIN      
        1 tcgatccgca ccgccgcatc cgccgccgcc gaatgatcgt gcttgtcggc gagccagtcc
       61 agcatcatcg ccgccgacag gatcatcgcc gtggggttcg ccttgccctg gcccatgata
      121 tcgggcgccg tgccatggca cggctggaag accgcatgct tgtctccgat atcggccgat
      181 ggcgcgatgc ctagcccgcc aatcaggccg gcggcgagat ccgagaggat gtcaccgaag
      241 atgttttcca tcaccatgac gtcgaagtcc cacggcttgc gcaccatcaa agccgcgcag
      301 gcatcaacat aaatgcgatc ggtcttcacg ttcggaaaag ccttggcgcg ctcgtcgaag
      361 atcgagcgga aataggcgta ggccttgaac gcattcgcct tgtcgacaca ggtcaggagg
      421 cccggttttc cgcgtttttt ccgccgttcg gcgagacgca gcgaaaaatc gaacagtcgt
      481 tgcgaggtct tgcgcgtgat caccaaagtc tcgcgcgcct cgtcctggtt gacgatgcct
      541 ttgcccatcg acgcgaacag gccctcagtc gattcacgga tcaggatgaa atcgatgccg
      601 cgcctatgcg cgtcgacgat cgggctcggc acgccgggga taagccgggc cggacgcacg
      661 ccggcgtaga gatcgaagat aaagcgaagc tcgacctgcg gcatgatctc ggtattgtcg
      721 ggatagcgca cgctcggcag gccacaggcg ccgaggagaa tggcgtcggc ttccgtgcat
      781 aacttgatgg tcgagtccgg catcgatttg ccggtctcct tgtagtgacc ggcgcctgct
      841 gcgccctcgg tgaaccggaa tttcaggctc ggcgttgtcg cttcgatccg ccgcaggatc
      901 tcgagcgaag gtgccatcac ctccggcccg atgccgtcgc cgggaagaac agcgatatgg
      961 aaggcggtgt ttgcagtcac aggttttccg gatggtgttg gagctgccgc ggcgctaatg
     1021 aatacgtccg cgcgttcacg cgcaagggat cattggggaa atgttatgcc acgctttgcc
     1081 gccaatctcg gctatctttt cactgaacgc cccttgctcg aacggatcga tgccgctgcg
     1141 gccgcgggct tcaaggccat cgaactgcaa tttccttatg atgtgcccgc cggcgccgtg
     1201 aaagccgcga tcatcaaaaa caacctgacc attctcggga tcaacacgcc acccggcgag
     1261 cgagagggcg agttcgggct tgccgcggtg cccggccgcg agaaagactg gcaggtcctg
     1321 tttgcgcgcg cgctcgatta cgcgagcgca ataggcggcg gcgccattca ctgcctggcc
     1381 ggcaaaatcg cccccgagca gcggccggcc gccgatcgtg tcttcatcga caatcttgtg
     1441 cgtgccgcgg atctggcagc gacgaagaac atcacgcttc tgatcgaacc gatcaacgcg
     1501 cgcgaccggc cgaattattt tctcaaccac gtcgagcacg ctgccgatgt gattgcgaag
     1561 accggaaagc cgaatgtccg gatgcagttc gatttctatc acgtgcagat agtgggcggc
     1621 gatctgatcc accgtctcga aaagttcctg ccggtgatcg gtcatctgca atgtgcggct
     1681 gtgccttcac ggcacgagcc tgatgagggc gaggtcaact atccggcgat cttcgaggcg
     1741 gtggatcgga tcggctacgg cggctggatc ggcgccgagt atcggccacg cgcgcgcacc
     1801 gaagccggcc tgggctgggc gcgtggttac ggcgtcgtcg cgacgcatta atccattatt
     1861 accaatcaaa cttcttgaaa actaacgctt tttggctttt cgggcttggc gggcattggt
     1921 agattgatgc catgtgggga cgcaaaaaag atcaggtcaa gagcgcggat agtccgcagc
     1981 cgctgaacga tgccgtgcgc gaagcacgca tcgaggcggc cgagcgctca ggcgtcgtcg
     2041 tcgacttgcg ggatgccgat gttgcacgac tggatctgct caacgatgcg cttggtccct
     2101 tgtttgatga agtcccgaat gacgtcgact tgttcgatcg cggtgtgagc aaaggcgaca
     2161 ccccgcgctt gtggatcgac gttgtcgcgc atgtcgagat gggccgtgac aagcggcaat
     2221 accgtttcgt gcaagacacg cgttacggtc gcgcggtgct tgcggaatcg tatgaagtgc
     2281 ccgcaatggc ggacgccatc aagcgttatg tcgcgcgccg gttggtcgag cgcgagcgcg
     2341 ctcttgccga cgatgccggg agcggtgttg cgcaggcggt cctgtatcgc ggtcgccgcc
     2401 ggcggcgctt gcgggccgta ggcatgttca tcttcggcgt ttttgtcggt atcgcgggtc
     2461 tcatcgcgct tgccctgctc acgcggccct aaccgatcag tttgatcttg tcctgctgca
     2521 cgatctcgcc cgccgagcca aggccgcgcg aagtcatctc gcagcgccag tcgccgc
//
LOCUS       DASSRL010000067         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_596842, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000067 DASSRL010000000
VERSION     DASSRL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596842"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /gene="glpD"
                     /locus_tag="VFL49_01340"
     CDS             complement(<1..1182)
                     /gene="glpD"
                     /locus_tag="VFL49_01340"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HET7910650.1"
                     /translation="MADFDLAIIGGGINGTGLARDAAGRGIRVLLVEMNDLGSGTSSA
                     SSKLIHGGLRYLEHGAFRLVREALSEREVLLRTAPHLVRPMRFMLPPLPGLRSALLLR
                     FGLLLYDVLGARRLLPASRTIDLTHHQAAHPLKREFRSGFEYSDCSVDDSRLVVLNAV
                     DAAERGAVIQTRTRCTRAERGDEWQLVLNDHGRRSVASARVLVNAAGPWIGAVAETII
                     RQPLARGVRLIKGSHIVVRRQAQNDYGYILEAADRRVVFVLPFADDFSLIGTTDENFV
                     GDLSAPTPTPQEIAYLCDVVNRYFRNVITPDEVVWSFSGVRALADDGSDRPEDVTRDY
                     ELVLDRSQDEAPLLTVYGGKITTYRRLAEAALARIGHYFGARRRWTAQSTLPGGEFPP
                     DA"
     gene            1425..2243
                     /locus_tag="VFL49_01345"
     CDS             1425..2243
                     /locus_tag="VFL49_01345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET7910651.1"
                     /translation="MAEHVAAGGIPASKGYSDLLRELLPGAMVDICYPGDPTALLPEG
                     ASLEGYDGIAITGSSLHVYNAGPEVMRQVELAQAALKTGTPIFGSCWGIQIITVAAGG
                     SVRKNPKGREIGFGRGIRLTEAGRKHPMYVGKLDVFNAPTVHLDEVEAVAPGTTVLAT
                     NAVSDVQSAEIRTNGSIAWAVQYHPEYPLREIAAIVRRIGPRLIEEGFFADKADIKTF
                     AQDLDALDRDPGCKRLAWRHGISKNVLDKKLRVGEVANWIELQVLPTRVKRGRG"
     gene            2262..>2794
                     /locus_tag="VFL49_01350"
     CDS             2262..>2794
                     /locus_tag="VFL49_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7910652.1"
                     /translation="MDFSGKHVVVTGGTGALGTAVVSVLIGGGAVCTVPYVHDAEAER
                     FPYRSQPQATLVKVSDLSDEAEVAKTYAGVKSLWASIHIAGGFAAAKFTDIDKAALMA
                     QIDSNLTSCFLCCRAAVKAMMPAGGGRIVNVAARPAIEPRSGAGMTAYTVAKAGVAAL
                     SMALAEEVAKDGILVNAV"
BASE COUNT          536 a          887 c          909 g          462 t
ORIGIN      
        1 tgcatcgggc gggaattctc cgcccggcag tgtggactgc gccgtccagc gccgtcttgc
       61 gccgaagtag tggccgatcc gcgccagcgc ggcctccgcg agccggcgat aggtcgtgat
      121 cttgccgcca tagacggtga ggagaggcgc ctcatcctga cttctgtcga ggacgagttc
      181 gtaatcgcgg gtaacgtctt ccggtctgtc cgagccgtcg tcggcgagcg cgcgcacgcc
      241 ggagaaagac cacaccactt catcaggcgt gatgacattg cggaaatacc gattgacgac
      301 atcacacagg tacgcgatct cctgcggcgt cggcgtgggc gcgctgagat ctccgacaaa
      361 attctcgtcg gtcgtgccga tcaggctgaa atcatccgca aacggcagca cgaagacgac
      421 gcgccggtcc gccgcttcca ggatatagcc ataatcattc tgggcttggc ggcgcacgac
      481 gatgtggctg cccttgatca accgcacgcc acgcgccaaa ggctgccgga tgatggtctc
      541 cgccaccgcc ccgatccagg gaccggccgc attgaccagc acgcgcgctg aggcgacgct
      601 tcggcgaccg tgatcgttga gcaccaactg ccattcatcc ccccgctcgg cgcgggtgca
      661 gcgcgtgcgc gtctggatga cggcaccgcg ttcggcggca tcgaccgcgt tgagcacgac
      721 caggcgggaa tcgtcgaccg agcagtccga gtattcgaat ccggaccgga attcccgctt
      781 cagcggatga gcggcctgat gatgcgtgag atcgatcgta cgggatgccg gcagcagccg
      841 acgcgcgccg agcacgtcgt acagaagcag tccgaagcgc aacagcagcg ccgagcgcaa
      901 gcccggcagc ggcggcagca taaagcgcat cggacgcacc aagtgagggg ccgtgcgcaa
      961 aagcacctcg cgctcgctca atgcctcgcg gacaaggcga aaggcgccgt gctccaggta
     1021 acgcaacccg ccatgaatca gtttgctgga ggctgacgag gtgccggagc cgagatcgtt
     1081 catctcgaca agcagcacgc ggatgccgcg accggcggcg tccctggcga ggcctgtgcc
     1141 gttgatgccg ccaccgatga tggcgaggtc gaagtcggcc attgcgcact gattcgcttt
     1201 ccggcgcagg atgagcggct tgcgccgcac actcaagaac gggaatgcat taaccccgca
     1261 tttgcgccat aaatcccgcc gcgattgcaa aactgcggct gtgccgccat atccaggaaa
     1321 gcgccgctca ttggtgcacg ccgggggcgg ctgtcagcga acctgaaacg ggaccgatgc
     1381 cctcgccacg cctgctcgtc gttgaaggaa attccccgca gaccatggcc gagcacgtcg
     1441 cagccggcgg gattccggcg agcaagggct attccgacct gctgcgggag ctgttgcccg
     1501 gagccatggt ggatatttgc tatcccggcg accctacggc cttgttgccc gaaggagcct
     1561 cgctcgaggg ctacgacggc atcgcaatca ccggctccag cctgcacgtc tacaatgccg
     1621 gcccggaggt gatgcggcag gtcgaactgg cgcaggcggc gctgaaaacc ggcacgccga
     1681 tcttcggatc atgctggggc atccagatca tcaccgtggc agccggcggg agcgtgcgca
     1741 agaacccgaa aggccgtgaa attggtttcg gccgcggcat ccgcctcacc gaggccgggc
     1801 gcaagcatcc catgtatgtc ggcaagctcg atgtcttcaa tgcgccgacc gtgcatcttg
     1861 acgaagtcga agcggtcgca ccgggcacga ccgtgctcgc caccaatgcg gtttccgacg
     1921 tgcagagcgc cgaaatccgc accaacggat ccatagcctg ggcagtccag tatcaccccg
     1981 agtacccgct gcgcgaaatt gccgccattg tccggcgcat cggcccgcgt ctgatcgagg
     2041 aagggttctt cgcggacaag gcagacatca agaccttcgc gcaggatctc gacgccctcg
     2101 accgcgatcc cggttgcaag cggctggcct ggcggcacgg catcagcaag aatgtgctcg
     2161 acaagaagtt gcgcgtcggc gaagtggcga actggatcga gcttcaggtc ttgccgacgc
     2221 gcgtgaaacg cgggcgcggc taggcaggtc gggcatccag gatggatttt tccggaaagc
     2281 acgtggtcgt taccggcggc accggcgcgc ttggcaccgc ggtcgtcagc gtgctgatcg
     2341 gcggcggcgc ggtttgcacg gtaccttatg ttcacgatgc cgaggccgag cgttttccct
     2401 atcgcagtca gccgcaagca acgctggtca aggtttccga tctgtccgac gaagccgaag
     2461 tggcgaagac ttatgcaggc gtcaaatcgc tttgggcatc gattcacatt gccggcggtt
     2521 tcgccgcggc aaaattcacc gatatcgaca aggccgcgct gatggcgcag atcgacagca
     2581 atctcacctc gtgttttctg tgctgccgcg cggcggtaaa ggcgatgatg cccgccggcg
     2641 gcggacgcat tgtcaacgtg gcggcacggc cggcaatcga gccgaggagc ggcgccggca
     2701 tgactgcgta tactgtcgcc aaggccggcg tagcagcgct cagcatggct ctggccgagg
     2761 aagtcgcaaa ggacgggatt ctggtgaacg ccgt
//
LOCUS       DASSRL010000068         6887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1593930, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000068 DASSRL010000000
VERSION     DASSRL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593930"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..963)
                     /locus_tag="VFL49_01355"
     CDS             complement(100..963)
                     /locus_tag="VFL49_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7910653.1"
                     /translation="MRDLSGNYRWLSLNTATVRKQGSLLDIIEACARHGIRAIDPWRD
                     QVAATGVDRAAQAIRDTGLELSGYCRGGMFTADAAHRNEARDDNRRAVDEAKALGAPC
                     IVLVVGGLPQYSRPGSMPSKNLAAAHSQVEDGIAELFEYARTAGMPLAIEPLHPMTAA
                     DRACVNTMRHALDLCDRIDPARSGLLGVAVDVYHVWWDFELMAQIERAGPRRLLAFHV
                     CDWLVPTVDTVNDRGMMGDGVIDIPRIRSAVEAQGFGGYVEVEIFSNRWWSVPMDEVF
                     ATLIARTKTVV"
     gene            complement(963..2153)
                     /locus_tag="VFL49_01360"
     CDS             complement(963..2153)
                     /locus_tag="VFL49_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7910654.1"
                     /translation="MNRMDTSLRTRAIRLPRADRSLEAFQLSEPKHFPERQGSKLNRI
                     AFAAAHVVADPNADNDPWIDCAIDWDRTIAFREHLWGLGLGVAEAMDTAQRGMGVDWP
                     TSLELIKRSVAAAKACNGLVFSGCGTDQLDPGNARGLDDVIRAYEEQIEAIETAGGRI
                     VLMASRALAKVARSTDDYAKVYGRILQSVREPVIIHWLGDMFDPALAGYWGSADADSA
                     MTTAVEIINEHAAKVDGVKISLLDKDKEIAMRRRLRQGVLMYTGDDFNYAELIAGDSQ
                     GYSHALLGIFDVIAPAASAALVRLAANDNDRFHEILAPTVPLSRHIFRSPTRFYKTGV
                     VFMAYLNGHQDHFTLVGGQESARSTVHLAEIFRLADKAGLLRDPDLAMGRMRTVLAAR
                     GIEA"
     gene            complement(2165..3313)
                     /locus_tag="VFL49_01365"
     CDS             complement(2165..3313)
                     /locus_tag="VFL49_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7910655.1"
                     /translation="MTTRRLGIIMNGVTGRMGTNQHLIRSILAIRAQGGVTLANGDRV
                     MPDPILIGRNADKTAALAKQHGVERWSTDLDKALANKDDVIFFDAATTQMRPALLEKA
                     IAAGKHIYCEKPIATTLAEAMKACQLAEKSGLKHGVVQDKIFLPGLMKLKFLRDSGFF
                     GRMLSVRGEFGYWVFEGDWGQPAQRPSWNYKQAEGGGIILDMLCHWRYVLDNTFAEVK
                     AVSCIGTTHIPQRWDEDGKAYKADADDAAYATFQLDGGIIAHINSSWCVRPYRDDLVT
                     FQVDGTHGSAIAGLHDCMMQSRQGTPRPIWNPDQKQTLDLYASWQPVPDFTTFDNGFK
                     VQWEMFIRHVAENAPYKYTLREGAKGLQLVECATRSWKERRWVDVPAL"
     gene            complement(3310..4680)
                     /locus_tag="VFL49_01370"
     CDS             complement(3310..4680)
                     /locus_tag="VFL49_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7910656.1"
                     /translation="MVPKIAIKAIDFFERRTPFRHPFRFGAVTVNEASQLFVHVSADV
                     DGKSSSGVTAELMVPKWFNKDPVLSAEETVDQLRRSAAIARPIYLKARPDTAFGLHAA
                     CYEPQVAECGKAGIPPLAAAFGAAEIDKAVLDALLRAVSTDFFAGMRSNIAGIDARLT
                     PDLTGTTIADFLASRIPSPRIAVRYTVGMLDTVDSIREAARGYRYFKLKLCGDPAKDR
                     ARLAEIVDALGETDYRASADANEQYQSLNDLRTLVDAIGSDRALVPLARRLLYIEQPF
                     ARDRTWNFDLRALAADAAFIIDEADDSYDAFPRARALGYRGVSSKSCKGIYKSLLNGS
                     RAALWSKSDDDFFITAEDLTCQAGLAVQQDTALVALHGLAHAERNGHHYVDGFANTPA
                     HEADAFLAMHPDLYERSDGTVHLSVRDGAIATGSLNVRGFAHALPSEQIGSALNQKNE
                     FKEFAS"
     gene            complement(4686..5492)
                     /locus_tag="VFL49_01375"
     CDS             complement(4686..5492)
                     /locus_tag="VFL49_01375"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910657.1"
                     /translation="MAANLRIVADAQSRRPVIELQGVSKTYRTADGEVPSLRPIDFTI
                     GEGEFVVVVGPSGCGKTTLLKMIAGLLPPSQGEIRVEGTPIVKPHGGVGIVFQTAMLL
                     PWRNVFRNVMMPVEVKGLPREEYAARAQALLKMVGLQGFENKFPWQLSGGMQQRASIC
                     RALVHDPKIVLMDEPFGALDAMTRERMNVELQRIQRETKKTVLLITHSIPEAVFLADR
                     VMVMSARPGNIAAVYEVSLRRPRSLEVLSDPAFIELTQTIRAHFSDAHLD"
     gene            complement(5504..6487)
                     /locus_tag="VFL49_01380"
                     /pseudo
     CDS             complement(5504..6487)
                     /locus_tag="VFL49_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(6525..>6887)
                     /locus_tag="VFL49_01385"
     CDS             complement(6525..>6887)
                     /locus_tag="VFL49_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7910658.1"
                     /translation="QGLKSVEPDMLDLARAMEARRLQIFRMVRFPHAMPAIFAGLKVS
                     ITLAVVGAVVGEFVGSNNGLGFVLQRSIGNFELPTMFAALVLLSLIGVVLFWVLDVIE
                     RIAIPWHVSQRQDFVATA"
BASE COUNT         1238 a         2140 c         2163 g         1346 t
ORIGIN      
        1 ccagccccgg gcacgacacg ggagcccggt accggaagag ccaagctacc ggggcgccgt
       61 taacgaatag tttagcatgc tggccgcggg cggttctgat cagaccacgg tcttggtccg
      121 cgcaatcagc gtcgcgaaaa cctcgtccat cggcacagac caccatcggt tggagaagat
      181 ttcgacctca acataaccgc cgaaaccttg cgcttcgaca gcagagcgga tgcgcgggat
      241 atcgatgacg ccgtcgccca tcattccccg gtcgttgacg gtgtctacag tcgggaccag
      301 ccagtcgcac acgtgaaagg ccaacagacg gcgggggccg gcgcgttcga tctgcgccat
      361 caattcgaaa tcccaccaca cgtgatagac gtcgacggcc acgccgagca gcccgctgcg
      421 cgccggatca atacggtcgc acaggtcgag ggcgtggcgc atcgtattga cgcacgcgcg
      481 gtcggcggcg gtcattggat gcagcggctc gatcgccagc ggcattccgg ctgtgcgggc
      541 atattcaaaa agctcggcga taccgtcttc aacctgtgaa tgggccgcgg cgaggttctt
      601 gctcggcatg ctcccggggc gcgaatactg ggggaggcca cccacaacca gtactatgca
      661 cggcgcgccc agcgccttcg cttcgtcgac ggcgcggcgg ttgtcgtcgc gcgcctcgtt
      721 acggtgggct gcgtcggcgg tgaacatgcc gccgcggcag tagccggaga gttcaagacc
      781 ggtgtcgcgg attgcctgtg ccgcacgatc gacccccgtg gccgcaacct ggtcacgcca
      841 ggggtcgatc gcgcgaatgc cgtgtcgtgc gcaggcctcg atgatgtcga gcagcgagcc
      901 ctgcttgcgc acggtcgcgg tgttgaggga cagccagcga tagttgccgc tcaggtcccg
      961 catcaggctt cgattccgcg cgcggcgaga accgtccgca tcctgcccat cgccagatcg
     1021 ggatcgcgca acagcccggc cttgtcggcg agccggaata tctccgcaag atgcacggtc
     1081 gagcgcgcgc tttcctgacc cccgaccagc gtaaaatgat cctggtgccc gttgagatag
     1141 gccatgaaca cgacgccggt cttgtagaag cgggtagggc tccggaagat gtggcgcgac
     1201 agcggcacgg ttggcgccag aatttcgtgg aagcggtcat tgtcgttggc ggcaagcctg
     1261 accagcgccg cggaagccgc cggtgctatg acatcgaata tgcccagcag cgcgtgcgaa
     1321 tagccctggc tgtcgccggc gatcagttcg gcgtagttga aatcatcgcc ggtatacatc
     1381 agcacgcctt gccgcaggcg ccggcgcatt gcgatctcct tgtccttgtc gagcagcgat
     1441 atcttcacgc cgtcgacttt cgcggcgtgc tcgttgatga tttcgacagc ggtggtcatg
     1501 gcgctgtcgg catcggccga gccccagtag ccggcgagcg caggatcgaa catgtcgccg
     1561 agccagtgga tgatcaccgg ctcgcgcacg ctctgcagaa ttcttccgta gaccttggca
     1621 taatcgtcag tcgaccgcgc caccttggcg agcgcgcgcg aggccataag cacgatccgt
     1681 ccgccggcgg tctcgatggc ttcaatttgc tcctcataag cacggatgac gtcgtcgagg
     1741 ccgcgcgcgt tgcctggatc gagctggtcg gtgccgcaac ccgagaagac caggccgttg
     1801 cacgcctttg ccgcggcgac agatcgcttg atgagctcca gcgaggtcgg ccagtcgacg
     1861 cccatgccgc gttgcgcggt atccatggcc tcagcgacgc caagaccgag tccccaaagg
     1921 tgctcacgga acgcgatggt cctgtcccag tcgatcgcac agtcgatcca tggatcattg
     1981 tccgcgttgg gatcggcgac aacgtgcgct gccgcgaacg caatgcgatt gagcttcgaa
     2041 ccctgccgtt cggggaagtg tttaggctcg gacagctgaa acgcttccag cgaacgatcg
     2101 gcgcggggca accggatcgc gcgcgtgcgc aaagatgtgt ccatgcggtt catcattagc
     2161 ctccttacag cgcggggaca tcgacccagc gcctttcctt ccagctcctg gttgcacact
     2221 ccaccagctg caggcctttg gcgccttcgc gcagcgtata tttgtacggc gcgttttcag
     2281 ccacgtggcg gatgaacatt tcccattgca ccttgaagcc gttgtcgaag gtcgtgaaat
     2341 ccggcaccgg ttgccacgat gcgtaaagat cgagcgtctg cttctggtcg ggattccaga
     2401 tcggccgcgg cgttccctgc cgcgactgca tcatgcagtc gtgcaggcca gcgatcgccg
     2461 agccgtgcgt accgtcgacc tgaaaggtca cgagatcgtc ccgataggga cgcacgcacc
     2521 acgacgagtt gatgtgggcg atgatgccgc catcgagctg aaacgtcgca taggccgcat
     2581 catccgcatc tgccttgtag gccttgccgt cttcatccca gcgctgggga atatgcgttg
     2641 tgccgatgca ggaaacggcc ttcacctcgg cgaaggtgtt gtcgagcacg taacgccagt
     2701 ggcacagcat atcgaggatg atgccgccgc cttccgcctg cttgtagttc cagctcggcc
     2761 gctgggccgg ctgtccccag tcgccctcga acacccagta gccgaactcg ccgcgcaccg
     2821 acagcatgcg gccgaaaaat ccgctgtcgc gcaaaaattt gagcttcatc aggccgggca
     2881 gaaaaatctt gtcctgcacg acgccgtgct tgaggcccga tttttcggca agctggcacg
     2941 ccttcatggc ctcggcgagc gtggttgcga tcggcttctc gcagtagatg tgcttgccgg
     3001 cggcgatcgc tttttcgagc aaagcaggcc gcatctgcgt ggttgctgca tcgaaaaaga
     3061 tcacgtcatc cttgttggcc agcgccttgt cgagatcggt cgaccagcgc tcgactccat
     3121 gctgcttcgc aagcgccgcg gttttgtccg cattgcggcc gatcaggatc ggatccggca
     3181 tgacgcggtc gccgttcgcg agcgtgacgc cgccctgtgc acggattgcg agaatggacc
     3241 ggatcagatg ctgattggtc cccatccggc cggtgactcc attcatgatg ataccgaggc
     3301 gtcttgtcgt cacgacgcga actccttgaa ttcgtttttt tgatttagcg ctgacccgat
     3361 ttgttcggat ggcaatgcgt gcgcgaagcc gcgcacattg agcgagccgg tcgcgatcgc
     3421 cccgtcacgg accgagaggt gcaccgtgcc gtccgatcgt tcgtagaggt ccggatgcat
     3481 cgcaaggaat gcgtcagcct catgagcggg cgtattggca aatccgtcaa cgtaatgatg
     3541 gccattgcgc tcggcgtgcg cgaggccgtg caaggcgacc agcgccgtgt cctgctgtac
     3601 ggcgaggccg gcctggcagg tcaaatcctc ggcggtgatg aagaaatcgt catcgctttt
     3661 gctccaaagc gcggcgcgtg agccattgag cagcgacttg taaattcctt tgcacgactt
     3721 ggacgaaacg ccgcgatagc cgagcgccct ggcgcgcgga aaggcgtcat agctgtcgtc
     3781 ggcctcgtcg atgatgaagg cggcatcggc cgccagcgcc cggaggtcga aattccaggt
     3841 cctgtctcgc gcgaagggct gctcgatata gagcagccgc ctggcgagcg gcacgagcgc
     3901 gcgatcgctc ccgatcgcat cgaccaatgt gcgcaggtca ttgaggcttt gatactgctc
     3961 gttggcgtct gcgcttgcgc ggtagtcggt ctcgcccagc gcatcgacga tctcggcgag
     4021 gcgcgctctg tcctttgccg ggtcgccgca gagcttgagc ttgaagtagc gatagccgcg
     4081 cgccgcttcg cggatgctgt cgacggtgtc gagcatgccg acggtgtagc gcacggcgat
     4141 gcgtggggag gggatgcggc ttgccaggaa gtccgcgatg gtggtgccgg tcagatcggg
     4201 cgtgagccgt gcgtcaatgc ccgcaatatt gctgcgcatg ccggcgaaaa aatcggtgct
     4261 gacggcccgc agcagagcat cgagcacggc cttgtcgatt tcggccgcgc caaaggcagc
     4321 cgccagcgga ggaatgcccg cctttccgca ttcggcgacc tgcggctcat agcatgcggc
     4381 gtgcagaccg aacgcggtgt cgggccgcgc cttcagatag atggggcgcg cgatcgcagc
     4441 cgacctgcgc agttgatcga cggtttcctc ggcggagagc accgggtcct tgttgaacca
     4501 cttcggcacc atcagttcgg cggtgacacc gctgctggat ttgccatcca catcggcgct
     4561 cacgtggacg aacagctgcg aggcctcgtt cacggtcacc gcgccgaaac ggaacggatg
     4621 gcggaacggc gtgcgccgct caaagaagtc aatggccttt atggcgatct tgggcaccat
     4681 gacggtcagt cgagatgggc gtctgagaaa tgcgcacgga tcgtctgcgt cagctcgata
     4741 aacgccggat cggacaggac ctcgagcgag cgcggccggc gcaaggaaac ctcgtagacg
     4801 gcggcgatat tgccgggccg ggccgacatc accatcacac gatcggccag gaagacggct
     4861 tccggaatgg aatgcgttat gagcaggacg gtcttcttgg tttcccgctg gatgcgttgc
     4921 agctcgacat tcatgcgttc gcgcgtcatg gcgtcgagtg cgccgaatgg ctcgtccatc
     4981 agcacgattt tcgggtcatg gaccagagcc cggcagatcg aggcgcgctg ctgcatgccg
     5041 ccggatagct gccaggggaa tttgttttcg aagccctgca ggcccaccat tttcagaagc
     5101 gcctgcgcgc gcgccgcgta ttcctcgcgg ggcaggcctt tcacctcgac cggcatcatc
     5161 acattgcgga atacgttgcg ccacggaagc agcattgcgg tctggaatac gatgccgacg
     5221 ccgccgtgcg gcttgacgat cggcgtgccc tcgacgcgga tttcgccctg gctcggcggc
     5281 agaaggcccg caatcatctt gagcagggtc gtcttgccgc agccggacgg gccgaccacg
     5341 acgacaaact cgccttcgcc gatggtgaaa tcgatcggac gcagcgaagg tacttcgccg
     5401 tcggcagttc gataggtttt cgagacgccc tgcagttcaa tgacagggcg tctcgactgg
     5461 gcgtcagcca cgattctcag gttcgcagcc attgccggcg tcttcagctg ttcccgccgg
     5521 gcaaataggc gtttgtgtag tagttcttgt agtcgtcctt cgccttcgcc tcgaggccgc
     5581 cgtactccac catgacgttc acggtatctt tcatgttggc gtcggtgacg ttgaacggcc
     5641 ggttctgctt gcccgaggga tccttgtaga gcgggatcgt cagctcaaat ccttttgtga
     5701 gcgtgtcgat cttgccgccc ttcggattgg cgttgaggat cgcctgcgca gcctctttcg
     5761 gatgcttgga ggcctactca agcgacttcg tggccgctgc catgaagcgc ttcaccaggt
     5821 ccttgttctc gttcagccag tccttgttca cgatgacccc ggagctcacc aggttgacgc
     5881 cgtagtcggc gaacttgatc gggaacgcgt cctttccggt cgcatccttg atcttcatgc
     5941 tctggtccat gacgtagccg agcagcagat cggcctggcc gttgatgacc gcattgagct
     6001 tggtggtggc gtcgccggag actgtcttga agtcgctttc cttcaggccg gtctttttca
     6061 ggaacagagg ccagatctgc gacatcgaat cgcccggcgt cgtcgccacc gtctttccct
     6121 tgatgtcttc gggcttgcgg atgtttttat cgacgaagcc catcacagac attgggttgg
     6181 tctgcaacgc gacgccgacc gcctcgacgg gcgcgccctt gacggcagca cgcatcatcg
     6241 tcggcacgtc gacataaccg aactgcacgg ttttcgccgc gaccacctgc gtgaccgtgg
     6301 ccgagccgcg gccttcctgg atctcaaggt cgatgccggc gtccttgaag tagccctttt
     6361 ccttgccgta gtagaatggc gcgtgctcgc catagacgta ccagttcagc atcagcgtca
     6421 ccttgtcggc cgcgaatgcc ggtccggcga gcagcaccgc gaacagtgcc gcaaaaaccg
     6481 atctcatcat gctctcctcc cttttgcagc gttaatagcg attgtcatgc ggtagcgacg
     6541 aagtcctggc gttggctgac gtgccacggg atagcgatcc gctcgatgac gtccagcacc
     6601 cagaagagca cgacgccgat cagcgaaagc aagacgagcg cggcgaacat cgtcggcagt
     6661 tcgaaatttc cgatcgatcg ctgcagcaca aatccgagac cgttgttcga tccgacgaac
     6721 tcgccgacga ccgcgccgac cacggcgagg gtgatcgata ccttcagccc ggcgaaaatc
     6781 gccggcatcg catgcgggaa gcgcaccatc cggaaaatct gcagccgcct ggcttccatg
     6841 gcgcgggcaa gatcgagcat gtcgggctcg accgacttca gcccctg
//
LOCUS       DASSRL010000069        10956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_701640, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000069 DASSRL010000000
VERSION     DASSRL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701640"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFL49_01390"
     CDS             <1..462
                     /locus_tag="VFL49_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7910659.1"
                     /translation="LKVADLQRALDFYCGVLGFTLTTSRPGAAFISAGGYHHHLGLNT
                     WESLGGSPPAPGTTGLYHTAILYPTRKLLADALRRLIMAKIPLEGASDHGVSEALYLR
                     DPDDNGVELYWDRPKDKWPKKPDGSLQMYTNPLDLHDLLAELDRPDKQTPR"
     gene            complement(459..638)
                     /locus_tag="VFL49_01395"
     CDS             complement(459..638)
                     /locus_tag="VFL49_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910660.1"
                     /translation="MPDIRSPQLILSIDLSGTDACCPRWGNGWVTVQVNVFENFPIFV
                     VMLTAAAMALARLSI"
     gene            complement(656..1453)
                     /locus_tag="VFL49_01400"
     CDS             complement(656..1453)
                     /locus_tag="VFL49_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7910661.1"
                     /translation="MYNFTFHRPTTVRQAAGLLARNPDAKLLAGGHSLLPVMKQRLAQ
                     PSALIDLALVEGLSGVEQKGRSIVIGAMTRHADAANSPVLQLLPGLASVPASVGDPQV
                     RNRGTIGGSIANNDPNADYPAACLGLGATIITNKRRIAADDFFTGMFSTALEDGEIIT
                     KVSFPIAKKAAYEKFKHPASGFALVGVFVSKRGADIRVAVTGAGANGVFRVKSFEDAL
                     KKRFAAKSIEGMTIPATGMNSDIHASPEYRAHLVGVLARRALAKATA"
     gene            complement(1470..3827)
                     /locus_tag="VFL49_01405"
     CDS             complement(1470..3827)
                     /locus_tag="VFL49_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7910662.1"
                     /translation="MSATGIGAAVRRKEDLRFITGRGQYTDDVIRSRETRAVFVRSPH
                     AHAKIKGIDTGAAKTMPGVIAVLTGTELANDKIGNLICGWMIHSKDGTPMKMAPHPAL
                     AVGKVNHQGDAVAVVIAETQVQARDAAEKVKVDYEPLPAVTDPAKAKSGQQIHDVAPK
                     NTIYEWSLGDAKAVDSAFSAAKHVTKLDIVNNRLVPNAIEPRAALAEYDGGTDQLTLW
                     NTTQNPHVSRLVIAAFVGMAPEHKLRVIAPDVGGGFGSKIFIYPEEVVCLWSARKVGR
                     PVRWTADRSESFVTDAHGRDHVTHAELALDANGKILAMRVKTIANLGAYMSTFSSSVP
                     TYLYATLLSGQYDIPQIYCEVDAVYTNTVPVDAYRGAGRPEATYVVERLVEVAAREIG
                     IDPADLRRRNFIKSFPHQTPVIMAYDAGDYNASLKKALDLADVKGFSKRKREAARQGK
                     LRGLGYSTYIEACGIAPSQAVGSLGCGVGLWESAEVRVNPTGSVEVLTGTHAHGQGHE
                     TTFAQLVSERLGIPFENVSIVHGDTDKVQFGMGTYGSRSGAVGMSAIVKALDKIEAKA
                     KKVAAHMLEAAEGDIVFKDGKFTVAGTDKSAAWGDVALNAYIAHKFAGQELEPGLKET
                     SFYDPTNFTFPAGCHICEVEVDRETGKTEIVNWTAVDDFGTVINPMIVEGQVHGGIAQ
                     GIGQALLESAVYDKDGQLVTGSLMDYCMPHAHDLPSFKVGLTETKAPSNPLGIKGCGE
                     AGAIAAPAAVINAITDAVGTEDIAMPATAQAVWAALKHAQPRQAA"
     gene            complement(3857..4354)
                     /locus_tag="VFL49_01410"
     CDS             complement(3857..4354)
                     /locus_tag="VFL49_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7910663.1"
                     /translation="MPAVSMTVNGKAVTGDVEARTLLVQFLREDLRLTGTHVGCDTSQ
                     CGACVVHIDGIAAKACTTLALQCEGAHITTIEGLADPNGPLHPMQEAFREHHGLQCGF
                     CTPGMIMAALDIVRRRGHDLDERTIREQLDGNICRCTGYHNIVKAIAAGAKAMAKGGA
                     RQAAE"
     gene            complement(4553..5002)
                     /locus_tag="VFL49_01415"
     CDS             complement(4553..5002)
                     /locus_tag="VFL49_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET7910664.1"
                     /translation="MAMSMTGEVQLPASRDVVWAKLNDPDVLKACIPGCEELNKTSET
                     EFQAVATTKIGPVKARFKGRVQLSDLNPPNGYRISGEGDGGVAGFAKGGATVALSDKD
                     GGTLLTYNVEAQIGGKLAQLGQRLVNGAAKKIADDFFQKFAASVAAS"
     gene            5160..5942
                     /gene="pcaD"
                     /locus_tag="VFL49_01420"
     CDS             5160..5942
                     /gene="pcaD"
                     /locus_tag="VFL49_01420"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HET7910665.1"
                     /translation="MPTINADDGCPLHVEVEGREGAPVLMLSNSLGTNLHMWDDQAPE
                     LAKHFRLVRYDRRGHGKSGAPKGPYSMERFGRDVLSIIDVLKIKTINWCGLSMGGMVG
                     QWLGANAANRIDKLVLSNTNFNYVDKAPWNDRIKFVREKGLGELVEPNMERWFTKDFR
                     TRAPQAISRMTEMFVATNPAGYIACCEAIREMDFTESNPRVAAPTLIIVGKQDPATPP
                     SAGEAIQKQIKGAKLASLDAAHIANVEVPKQYTETVLNFLRA"
     gene            5954..6343
                     /gene="pcaC"
                     /locus_tag="VFL49_01425"
     CDS             5954..6343
                     /gene="pcaC"
                     /locus_tag="VFL49_01425"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HET7910666.1"
                     /translation="MDDKERYDRGMAKRRKVLGNAWVDRANARKTEFNEEFQDFITRT
                     AWGEIWTRPHYDERTRRILVIGTMLALDKWEEFRMHVRAALTEANFTPDDIKEIILQQ
                     AIYCGVPAANHAFKEAADVIAEVAKSK"
     gene            6427..7506
                     /locus_tag="VFL49_01430"
     CDS             6427..7506
                     /locus_tag="VFL49_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7910667.1"
                     /translation="MAKIKVRKGMPSVQLDKREFKKRFLARFYDPEFESLAPELDKIS
                     ETAWKTYDSYHKSPRTRKAGQGFVDPDFDAPIEWLQTRANIQAAETVQKNPKSPSRIL
                     IVNGSSRNDQTCPGEVSKTWRMVTLAKQVIERIRGFEVDVLDLSRLAAEYGKVIYPCK
                     ACVSTAQPLCHWPCSCYPNHALGQAGDWMAEIYPKWVAAHGVMIVCPVNWYQAPSSLK
                     LMIDRLVCADGGNPDITSTGGKDPVKAKRLELAGWPYPRHLAGRVFSVVVHGDAAGTE
                     NLRRILTDWMSDIGMIPSGHMALVDRYVGYLTPYATSHDDLDKDHDFQDDVRNAALTL
                     VQAVKDRRSGKLQPADRGLHEARPK"
     gene            complement(7671..8207)
                     /locus_tag="VFL49_01435"
     CDS             complement(7671..8207)
                     /locus_tag="VFL49_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910668.1"
                     /translation="MQKISTATASIIVLIALYFTLSWGYEALRILTSPSYGFEDVWRS
                     QFLFGIGRLFDLGPTGLVKLAAFFGTLKLAVACICALHIADRARCFMRGQPNSEILEA
                     GLILVVAISIASVGPASWAQSTDLMREHTYQLLFAALATGLCIFERSYTHRAQTVEAK
                     PQSVVDATATQAAPRLHV"
     gene            8465..9844
                     /locus_tag="VFL49_01440"
     CDS             8465..9844
                     /locus_tag="VFL49_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7910669.1"
                     /translation="MRDPRFVLLATSLGVLLAQIDTSVVNLGLKSIAGDLNAGVSEMQ
                     WVIDAYNVVYASLLLTGGTLGDIYGRRRIFLFGIALFTVGTVLCALAPNAAALIAGRT
                     ISGLGAAFALPMSLVLLTIAYPRREERAHAMGIWASCNGLAFIIGPTLGGWLVDSVGW
                     RSIFYMSLPACAAALLLTMYAIEESVEPEGRRLDLPGQVLAIVALGGFAFAAIEGSHW
                     GWTTPLFAVLTIAFLATIAFVWVEARTPGPLLPLSFLGQPVFSAALAVAGLMTFGMYA
                     LLFIMPLYFQTIRGATPFFAGLELLPMSVSFVVVSQLVGYLTNNLGPRVVMATGMACM
                     GFGALAIAFISGQTSLVLIELALFVVGIGLGLNTAPVNGVAVAAVPAARSGTASGMLN
                     TARMVGATMGVAILGSVFAAYAGQEANVAASFMPGLRAAMIAAGLAELFGGAVAAVFI
                     RNDSLHAKK"
     gene            complement(9846..10739)
                     /locus_tag="VFL49_01445"
     CDS             complement(9846..10739)
                     /locus_tag="VFL49_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7910670.1"
                     /translation="MGKVSALPLFHLYGDPPDDSAFDFIHIETIASRSSVHDWTIRAH
                     RHRNLFQILLIEKGGGEMSFETATARFAAPAVILVPATTAHGFRFTPQVTNGWVVSFT
                     EDVADALGDQSGEALARLKAVATDPILPLADATETRRLSALCADLHEEDSLAREGHRL
                     AMRGLLALIAIEVVRLAVSRARSGAVTLSRYDVRVDELRRLVDEHFRKERLISFYAKT
                     LSMTPDRLNDHVKRATGVTAGHLIRQRVLTEAKRQLVFTNQAIHEIAYDLAFSDPSHF
                     TRFFRKQTGMTPQAFRDGRGG"
BASE COUNT         2179 a         3310 c         3258 g         2209 t
ORIGIN      
        1 ctgaaggtcg ccgacctgca aagggcgctg gatttctatt gcggggtgct gggatttacg
       61 ttgaccacca gccggcccgg ggcggctttc atatcggccg gcggctacca tcatcatctc
      121 gggctcaata cctgggaaag cctgggcgga tcaccgcctg cgcccggcac cacgggtctt
      181 taccataccg ccattctcta cccgacccgc aagctgctcg ccgacgccct gcgccggctt
      241 atcatggcga agatcccgct tgagggcgcc agcgatcacg gagtgagcga agcgctctat
      301 ctgcgtgatc ccgacgacaa tggcgttgag ctctactggg atcgcccgaa ggacaaatgg
      361 ccaaaaaagc cggacggctc gttgcagatg tacacaaatc cgctcgacct ccacgatcta
      421 ctcgcggagc ttgaccggcc ggacaaacag acgccgcgct aaatgctcag gcgcgccagc
      481 gccattgccg cggcagtcag catcaccacg aagatcggaa aattctcgaa cacgttgacc
      541 tgcacggtta cccagccgtt gccccaacgc gggcaacatg cgtcggttcc tgagagatca
      601 atgctcagga ttaattgtgg actccttatg tcgggcaacc gacagtgagt accgctcagg
      661 ctgtcgcttt cgcaagtgcg cgccgcgcca aaacgccgac caaatgcgca cgatattccg
      721 gactggcatg aatgtcgctg ttcatacccg ttgccggtat cgtcatgcct tcgattgact
      781 tggcagcgaa gcgcttcttg agcgcgtctt cgaacgattt cacgcgaaat acgccgtttg
      841 cgccggcacc ggtcactgcc acacggatat cggctccccg cttcgagaca aacacaccga
      901 ccaaagcgaa gcctgaggcc ggatgcttga atttttcgta ggctgccttc ttggcgatcg
      961 gaaagctcac cttggtaatg atctcaccgt cctcgagcgc tgttgagaac attccggtga
     1021 agaaatcatc ggccgcaatg cgccgcttgt tggtgatgat agtcgcgccg aggccaaggc
     1081 acgctgccgg ataatcggcg ttcggatcgt tattggcgat cgagccgcca atcgtgccgc
     1141 gattgcgcac ctgcggatca ccaaccgacg caggcaccga tgcgaggccg ggcaataact
     1201 gaagcaccgg agaattggcc gcgtcggcat gacgcgtcat cgcgccgatc acgatggacc
     1261 gacccttctg ctcgacgccg ctcaatcctt cgaccagcgc caaatcgata agcgcggacg
     1321 gctgcgccaa acgctgcttc atcaccggca agagcgaatg cccgcccgcc aggagcttgg
     1381 catcagggtt gcgggccagc agaccggccg cctggcggac agtcgtcgga cgatggaaag
     1441 tgaaattgta catttaaaat ctcccgtaac taggcggcct gacgcggctg cgcatgtttg
     1501 agcgctgccc aaaccgcctg cgcggtcgcg ggcatcgcga tgtcttctgt gccgactgca
     1561 tctgttatgg cgttgatcac ggctgcgggc gcggcgatcg caccagcctc gccgcagcct
     1621 ttgataccga gcgggttgga tggcgccttt gtttcagtaa ggcccacctt gaaagacggc
     1681 aggtcgtgcg catgcggcat gcagtagtcc atcagtgagc cggtcacgag ctgaccatct
     1741 ttgtcgtaga ccgcgctttc gagcagggcc tgcccgatcc cctgggcgat gccgccatgc
     1801 acctgccctt cgacgatcat aggattgatc acggtcccga aatcgtcgac ggcggtccaa
     1861 ttgacgatct cggtcttgcc ggtctcgcga tcgacttcga cctcgcaaat atgacagccg
     1921 gcggggaacg tgaaattggt cggatcgtag aagctcgttt ccttcagtcc gggctcgagc
     1981 tcctgcccgg cgaatttgtg ggcgatatac gcattgagcg cgacatcgcc ccatgccgcc
     2041 gatttgtcgg tgcctgcgac cgtgaacttc ccatccttga agacgatgtc gccttccgcc
     2101 gcttccaaca tgtgtgcggc gactttcttc gccttggcct cgatcttgtc gagcgccttc
     2161 acgatcgccg acatgccgac cgcgccggag cgcgaaccat aggtgcccat tccgaattgc
     2221 accttgtcgg tgtcgccatg cacgatggaa acattctcga atggaatgcc gagccgctct
     2281 gacacgagtt gcgcgaacgt cgtttcgtga ccctgcccgt gagcgtgcgt gcctgtcagg
     2341 acttcgacgc tgccggtcgg attgacgcgt acttcggcgc tttcccacag gccaacgccg
     2401 catcccagcg agccgacagc ctgcgacggt gcgatgccgc aggcttcgat ataggtcgag
     2461 taaccgaggc cgcgcagctt gccctgacgc gccgcttcgc gcttgcgctt gctgaagccc
     2521 ttaacgtcag cgagatcgag cgctttcttc agagaggcat tatagtcgcc ggcatcgtaa
     2581 gccatgatga ccggcgtttg atgcggaaat gacttgatga agttgcgccg gcgcaggtcg
     2641 gcgggatcga ttccaatctc ccgcgccgct acttcaacca gccgctcaac gacgtaggtg
     2701 gcttcgggac ggccggcgcc gcggtaggca tcgaccggca ccgtgttcgt gtagaccgcg
     2761 tcgacttcac aatagatttg cggaatatcg tactggcccg acagcagcgt cgcgtagaga
     2821 taggtcggga cggacgacga gaacgttgac atataggcgc caagattggc gatcgttttc
     2881 acgcgcatcg ccaggatttt tccattggcg tcgagcgcga gttcggcatg ggtgacgtga
     2941 tcgcggccat gcgcatctgt cacgaatgac tccgaccggt cggccgtcca tctcaccgga
     3001 cggcccacct tgcgtgcgga ccacaaacag acaacctctt caggatagat gaatatcttg
     3061 gaaccaaagc cgccgccgac gtctggcgca atgacgcgca acttgtgctc cggcgccata
     3121 ccgacgaaag cggcgatgac cagccgcgaa acgtgcggat tctgggttgt gttccagagc
     3181 gtcagctgat cggtgccgcc gtcatactcg gcaagcgcgg cccgcggctc gatagcattg
     3241 ggcacgagcc ggttattgac gatatcgagc ttggtgacat gtttggcggc gctgaatgcg
     3301 gagtccacgg ccttcgcgtc gccgagcgac cattcataaa tcgtgttctt gggcgcgacg
     3361 tcgtggatct gctggcctga cttggccttt gcaggatccg taaccgccgg aagcggctca
     3421 tagtcgactt tcactttttc agcggcatcg cgcgcctgca cttgcgtctc ggctatgacg
     3481 acagcgaccg catccccctg atggttgact ttgccgacag ccagcgccgg atgcggcgcc
     3541 atcttcatcg gcgtgccgtc cttggaatgg atcatccagc cgcagatcag gttgccgatc
     3601 ttgtcatttg ccagttcagt acccgtgagt acggcgatga cgcccggcat cgttttggct
     3661 gcgcccgtgt cgatgccttt tatctttgca tgcgcgtgcg gggagcgcac aaataccgca
     3721 cgcgtttcgc gagagcggat cacgtcgtcg gtgtattggc ccctgcccgt aatgaagcgg
     3781 agatcttcct tccggcgcac cgccgcgccg atacccgtcg cgctcatggc ggtctcctcc
     3841 cgaaataatt tttacgctac tcggcggcct gacgagcgcc gcctttggcc atggcttttg
     3901 ctcccgcggc aatcgccttg acgatgttgt ggtagccggt gcagcggcag atattgccgt
     3961 cgagctgttc gcgaatggtg cgttcatcga ggtcgtggcc gcgacggcgc actatatcga
     4021 gtgcagccat gatcatgccg ggcgtgcaga agccgcattg caggccgtgg tgctcgcgga
     4081 aagcctcctg catcggatgc agcggtccgt tggggtctgc caagccttcg atggtcgtga
     4141 tgtgggcgcc ctcacactgg agggcgagcg tggtgcacgc cttggcagca ataccgtcaa
     4201 tgtgaaccac gcaggcgcca cattgagacg tgtcgcaacc gacgtgcgtg ccggtcagcc
     4261 gcaagtcctc gcgcagaaat tgcaccagaa gtgtgcgcgc ctcgacatct ccggtgacgg
     4321 ctttgccatt caccgtcatt gacacggcag gcatgaagct tcctcccatt ttggggccga
     4381 aggccctctt ttcgccccca atcggccatt ggcggcggtt cgattgtcga atcgtgccga
     4441 ttttggcccg atcgctactt ggaacaggaa taagtctgaa ccccgattgc gggtcgggtc
     4501 aagggattca gggtgggaaa accgcctcaa actgcagtga ttgagatttc cgtcaggagg
     4561 ccgcgacgga tgcggcaaat ttctggaaaa aatcgtcggc gatttttttg gcggcgccgt
     4621 tgaccagacg ctgcccgagc tgagccagct tgcctccgat ctgcgcctcc acgttgtagg
     4681 tcagcagcgt gcccccgtct ttgtcggaga gcgccacggt cgcaccgcct tttgcaaagc
     4741 cggcgacacc gccgtcaccc tcgccggaaa tcctatagcc attaggaggg tttaggtcgc
     4801 tgagttgcac tcggccttta aatcgcgcct tcaccgggcc gatttttgtg gtcgcgacag
     4861 cctgaaattc tgtctccgaa gtcttgttaa gctcctcgca acccgggatg caggccttga
     4921 gaacgtcagg atcattgagt ttcgcccaga ccacatctcg gctcgccgga agctggactt
     4981 cacctgtcat tgacatcgcc atccggcatc tcctttacga ttgatctgag gtttccggcc
     5041 acccgactta agccttcggc acggccatga aaagagcttt gcaggccacc taacccgcat
     5101 tatggctttc cgccacggca agaccgcaaa tcaaaacgcg tctttagggg aggatcttaa
     5161 tgccgacgat caacgcagat gatggctgtc cgcttcatgt ggaggttgag ggccgcgaag
     5221 gcgcccccgt gctgatgctg tcaaactcgc tcggcaccaa cctgcacatg tgggacgacc
     5281 aggcgccgga gcttgcaaag cacttccggc tggtgcgtta cgaccggcgc ggccacggca
     5341 agtccggcgc acccaaaggc ccctattcga tggagcgctt cggccgcgac gtgctttcga
     5401 tcatcgacgt gctgaagatc aagacgatca actggtgcgg actgtcgatg ggcggcatgg
     5461 tcggccagtg gctgggagcc aacgcggcaa accgcatcga caagcttgtc ctctcgaaca
     5521 ccaactttaa ctacgtcgac aaggccccct ggaacgaccg catcaaattc gtaagggaga
     5581 aaggtcttgg cgaactggtg gaaccgaaca tggagcgatg gttcaccaag gatttccgga
     5641 cgcgcgcacc tcaagcgatc tcccgtatga ccgaaatgtt cgtggccacg aatcctgccg
     5701 gttacattgc gtgctgcgaa gccatccgcg aaatggattt caccgaatcc aatccgcgcg
     5761 tggcagcgcc gactttgatc atcgttggca aacaggatcc ggcaacgccg ccttccgccg
     5821 gcgaagcgat ccagaagcag atcaagggtg ccaagctcgc cagcctcgac gccgcgcaca
     5881 tcgcgaatgt cgaggtgccg aaacaatata ccgagacggt tctcaacttc ctccgcgcct
     5941 gacagagacg gccatggacg acaaggaacg ctacgaccgc ggaatggcca agcgccgcaa
     6001 ggtgcttggc aacgcctggg tcgatcgtgc gaacgccagg aagacggagt tcaacgaaga
     6061 atttcaggat ttcatcacgc gcaccgcgtg gggcgagata tggacacgcc cgcactatga
     6121 tgagcgcacc cgtcgcatcc tggtgatcgg caccatgctt gcgctcgaca agtgggagga
     6181 gttccgcatg catgtgcgcg cggcgctgac agaggcgaat ttcaccccgg acgacatcaa
     6241 ggaaatcatc ctgcagcagg cgatctattg cggagtgccg gccgctaatc acgctttcaa
     6301 ggaagcggcc gatgtcattg cggaagtggc aaaatcgaag tgaccgtcac gccaaggtga
     6361 cgggctcgtc atttcgagtt gaaaacgctt caatgctagc catggcccac acgatgggga
     6421 gcggtcatgg ccaagatcaa agtgcgcaag ggtatgccga gcgtccagct cgacaagcgg
     6481 gaatttaaga aacgattcct ggcccgattt tacgatccgg aatttgagtc attagcgcct
     6541 gagctcgaca agatttctga gaccgcgtgg aaaacctacg acagctatca caagtcgccg
     6601 cgcacgcgga aagccggaca gggcttcgtc gatccggatt tcgacgcgcc gatcgaatgg
     6661 ctgcagacac gtgccaatat ccaggccgcc gagacagttc aaaaaaaccc gaagtcaccc
     6721 tcgcggatcc tgatcgtcaa cggctcatcg cgcaacgatc aaacgtgccc gggcgaagtt
     6781 tcaaaaacct ggcggatggt tacgcttgca aagcaggtca ttgaaagaat ccgcggattt
     6841 gaagtggacg tcctcgacct gtcacgtctc gcagccgagt acggaaaagt gatctatccc
     6901 tgcaaggcat gcgtctcgac ggcacaaccg ctttgccact ggccgtgcag ctgctatccg
     6961 aaccatgcgc tcggacaggc cggcgactgg atggcggaga tctatccgaa atgggtcgcc
     7021 gcgcatggcg tcatgatcgt gtgtccggtt aactggtacc aggcaccctc gtcgctcaag
     7081 ctgatgatcg accgtctggt ctgcgcagac ggcggcaacc ctgatatcac ctcgaccggc
     7141 ggcaaggacc cggtgaaagc caagaggctg gagctggcag ggtggcccta cccgcgtcat
     7201 ctggccggcc gcgtcttttc ggtcgtggta cacggcgacg cagccggaac ggaaaatctc
     7261 cggcgtattc ttaccgattg gatgagcgac atcgggatga ttccgtcggg ccacatggcg
     7321 ctcgttgatc gttacgtagg ctatctcacg ccctacgcga ccagtcacga cgatctggac
     7381 aaggaccacg actttcagga cgacgtgcga aatgccgcgc tgactttggt acaggcggtc
     7441 aaggacagac gttccggcaa gctacaaccg gcagatcgcg gcctgcacga ggcgcggccc
     7501 aagtgaccgc gcgttttata tcgccctaag cacgcgcgat actgtctcca gtttttcgct
     7561 gagaaccgac aaatattcat gtgcgacgag ccacacgaga ctgaaacgca tcccccacag
     7621 cagggccatg gcaatgagtc gggcgggcat tgctaccggc cgcgacagcc tcagacgtga
     7681 agccgaggag ctgcctgtgt tgcggtcgcg tccacgacgg attgcggctt cgcttccaca
     7741 gtctgggccc ggtgcgtata gctgcgctcg aatatgcaca atccggtcgc aagcgccgca
     7801 aatagcaact gataggtgtg ctcccgcatc aaatcggtgc tctgcgccca cgacgccgga
     7861 ccgactgacg cgatgctgat cgctacgacc agaatgagtc cggcttccag aatttctgaa
     7921 ttaggctgcc cgcgcatgaa gcaacgcgcg cggtctgcga tgtggagagc gcagatgcag
     7981 gcgacggcaa gcttgagcgt tccaaaaaag gctgcaagct tgaccagccc tgtcggaccg
     8041 agatcgaaca gccggccgat gccgaaaaga aactgcgagc gccagacatc ctcgaaaccg
     8101 taactcggcg acgtcagaat ccggagcgcc tcatatcccc aggacagcgt gaaatagagg
     8161 gcaataagaa cgataattga tgcggtggcc gtggaaattt tttgcacgga ttcctccctg
     8221 accagggcct acggctaatc gtttcaaatt ggaaattcaa aagaaaccga aattttacct
     8281 taccgacggg ccgctggagg cccacgcatt aaccacgcac ctgccgattc cgaaccaaaa
     8341 ctttcatgga aaacgggtgg gactttggat tcgcggcgcc tacatccggt tttcgcgtgc
     8401 tcaaagcccg acctggttaa agatgaaacc tatgacagca acgacggcga agatgaacgg
     8461 cgcaatgcgc gacccccgat tcgtgctgct tgcaacgtct ctgggcgtgc tgctcgcaca
     8521 aatcgacaca tccgtcgtca atctcggcct gaaaagcatt gcgggggacc tgaacgccgg
     8581 cgtcagcgaa atgcagtggg ttatcgacgc atacaacgtc gtctacgcga gcctgctgct
     8641 gaccggcgga actctcggcg acatttacgg ccgccggcgt attttcctgt tcggcattgc
     8701 cctgtttaca gtcggtactg ttttatgtgc gcttgcacca aacgcggcag ccttgatcgc
     8761 cggccgtacc atcagcggac tcggcgcggc gtttgcgctg ccgatgtcgc tggtgctgtt
     8821 gacgatcgcc tatccgcgcc gcgaggaacg cgcgcatgcg atgggcattt gggcgagttg
     8881 taacgggctc gcttttatta tcggtccgac tctgggcggc tggcttgtcg acagcgtcgg
     8941 ctggcgcagc atattttata tgagcttgcc cgcttgcgcg gcggcgctgc ttcttacgat
     9001 gtacgcaatc gaagaaagcg tcgagcccga aggccgccgg cttgatctgc ccggacaggt
     9061 tctggccatc gtcgcgctcg gcggctttgc ctttgccgcg attgaaggtt cgcactgggg
     9121 ctggacgacg cctctcttcg cagtgctgac gattgcattc ctcgcaacaa tcgcgttcgt
     9181 gtgggtggag gcacgcacac cggggccgct gttgccgttg tccttcctgg gccagccggt
     9241 cttttcagcg gcacttgctg ttgccggcct gatgacattc ggtatgtacg ccctcctctt
     9301 catcatgccg ctctattttc agacgatccg cggcgcgacc ccgttcttcg ccggactcga
     9361 gttgctgccg atgtctgtaa gtttcgtcgt cgtatcgcag ctggtcgggt acctgaccaa
     9421 caatctcggg ccgcgcgtcg ttatggcgac cggaatggca tgcatgggtt tcggcgcgct
     9481 cgcgatcgcg ttcatttccg gccaaacgag tctcgtcttg atcgaattgg ccctctttgt
     9541 cgtcggtatc ggactcggcc tgaataccgc ccccgtcaac ggcgttgcgg tcgccgcagt
     9601 gccggctgcg cgatccggaa ccgcatccgg catgctcaac acagcacgca tggtcggcgc
     9661 aacgatgggg gtcgccattc tgggttcggt ctttgccgct tatgcaggtc aagaagcgaa
     9721 tgtagccgcg agcttcatgc ccggcttacg cgcggccatg attgctgccg gattggcgga
     9781 actgttcggg ggcgcagtcg ccgccgtctt catccggaac gactccctgc acgcaaaaaa
     9841 ataattcagc cgccgcgtcc gtcgcggaac gcctgcgggg tcatgccggt ctgcttccgg
     9901 aaaaaccgtg tgaaatgcga cggatcggaa aaggcgagat cataagcgat ttcgtgaatt
     9961 gcctggttgg tgaacacaag ctgtcgtttc gcttcggtga gcacgcgctg gcgaatgaga
    10021 tggcccgcag tgacaccagt cgcgcgtttg acatgatcgt tgaggcgatc aggtgtcatg
    10081 gagagcgttt tggcataaaa gctgatgaga cgttccttgc ggaaatgctc gtccacgaga
    10141 cggcggagtt cgtcgacgcg cacgtcgtat cgcgacagcg tcactgcgcc gcttcgcgcc
    10201 cggctcaccg ccagacgaac cacttcgatg gcgatcagcg cgagcaagcc ccgcattgca
    10261 agtcggtggc cttcgcgcgc gagcgagtct tcctcatgca gatcggcgca tagtgccgaa
    10321 agacgcctgg tctctgtcgc gtccgcgagc ggcagaattg gatcagtcgc gaccgctttg
    10381 aggcgcgcaa gcgcttcacc cgactgatcg ccgagggcat cggcgacgtc ctctgtgaag
    10441 ctcactaccc agccgttggt tacctgtgga gtgaaacgga atccatgcgc cgttgtggcc
    10501 gggacaagaa tgacggcggg cgccgcaaag cgggccgtcg cggtttcaaa actcatctcg
    10561 cccccgccct tttcaatcag caggatctgg aacaggttgc ggtggcggtg cgcacggatc
    10621 gtccaatcgt ggacggacga gcgtgatgcg atcgtttcga tgtgaatgaa gtcaaaggcg
    10681 gagtcgtcag gcggatcgcc gtagaggtga aataacggca gtgccgaaac cttgcccatg
    10741 cgtccgagca tattcgagcc ggttatcgat agctatggcg gtagaaaatg cgaagccccg
    10801 cacgttcgtg cggggcttcc gaacctactc gagtgaatca ggggtttatc ggctctgctg
    10861 gccctactgc tgaccgcctt gctgctgctg accaggcttc tgctggccgc cctgctgctg
    10921 ttggccaccc tgaccaggct tctgctgacc gccctg
//
LOCUS       DASSRL010000070         5954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_474305, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000070 DASSRL010000000
VERSION     DASSRL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474305"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFL49_01450"
     CDS             complement(<1..947)
                     /locus_tag="VFL49_01450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7910671.1"
                     /translation="MKSDHSPSDINRRAVISSIALLPASSEALFSTSALAQTTSGAPL
                     ASWNDGAAKQAILDFVRATTDQASPKFLPPEDRIATFDQDGTLWVEHPIYSQVVYCLD
                     RVPAVVETDPKLKNVEPFKTVLSGNREAIAKLPLHDLEKILAATLTGMSVDDFEAEVK
                     KWLTSAKHERWKRPYTELIYQPMLEVMQYLRSNGFKTYIVTGGGQDFVRVYSNQVYGI
                     PVEQVVGTVGGTKFGYHKDGTPFLTKEPKLLLNDDNAGKPEGIHLMIGRRPYAAFGNS
                     TGDQEMLEYTGAGDGARLMMLVLHDDAKREYAYGPATGLP"
     gene            complement(983..1732)
                     /locus_tag="VFL49_01455"
     CDS             complement(983..1732)
                     /locus_tag="VFL49_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910672.1"
                     /translation="MRKNDRRAVLTALLGSVAGAVLYKSPVLAQPGSVQPVGAGEPVT
                     AIDTLLDPDATMIQHAVAANERLRRSFPEGFALGKEHQPHISVLQRYVKTQDLNKVYD
                     AVGKVLAGEKPATWKLKAYKYYFIPWKNIGLAGIVVEPTDDLIRYQQKLIDVVTPFTV
                     ETGTASAFVTTKEDPEINQPTIDYVANFVPNETGKKFNPHVTIGLASQDYLKKMLDEK
                     FEAFTFSPIKASVYHLGNFGTARKLLKSWEL"
     gene            complement(1891..2301)
                     /locus_tag="VFL49_01460"
     CDS             complement(1891..2301)
                     /locus_tag="VFL49_01460"
                     /inference="COORDINATES: protein motif:HMM:NF042549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HET7910673.1"
                     /translation="MSDESRLKLLRIALVVIGLFAIFGFYPLTIVWSSGWTWGHGHSH
                     YLLMIIGVYATLGVFLLIASRDPYANKSLIWFTVWSSVVHGIIMGVQGLTDAAERGHL
                     VGDAPALILVAIILAVLMPRTATVAVQAPRTAQT"
     gene            complement(2280..3569)
                     /locus_tag="VFL49_01465"
     CDS             complement(2280..3569)
                     /locus_tag="VFL49_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910674.1"
                     /translation="MLTSRGNPLAEVDGVLADDPQFIFAHCVRATILLRGDHDAARTK
                     LVASVTAIEATCPDINDPARRHASAARAWLEGDQALAVERYGAILIDHPRDILALAVA
                     HTLDFRLGQRRMLRDRVAQVMPEWNETLPGYASVLAMYAFGLEENGQYRRAEEIARRA
                     FALDPRHPSVIHVIAHVMEMQGRDQEGLEFLAETEPVWKDTTGISVHLAWHRALFHLD
                     ADDPKSALAVYDAQIENTREPGMSELADASALLWRLDLLNFRVGERWMLLADRWQKHD
                     LTGVRPFYVAHAMMALAAAGRAAEVQRIFDALPHVETSGAMSEDALLLQLCKALLAFA
                     HSDYASAVKTLARVRHLAHRCGGSLAQCDLIHLTFTEAAFRARKVNLARALVAERTAQ
                     KPVSELNRVLQRRLAGQKATPQGIIEKETQTCLMKVA"
     gene            3956..4945
                     /locus_tag="VFL49_01470"
     CDS             3956..4945
                     /locus_tag="VFL49_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910675.1"
                     /translation="MKTGGSVKRRKFLALLAGAAATWPVSSRAQQLPKLTTVGVLASQ
                     LLPPVRRLTPRLQELGYTEGQNLRIEYRFAEGHDNRYPSLAAELAALPVDLIVTSGTP
                     AALAAKRATSTIPIVMATIGEAVSTGVVSNLARPGGNITGFTALNLELEGKRLALLKE
                     LLPHLSRVGILANRANPLFDVSLRNLRPAANALGLTLDIFNVRDKDDIESALLRIDHA
                     HPDGVVVAADTLLLSKRGEIAASLAKGRFPAIYAFREYADVGGLMIYGANLGILFERT
                     ADYVDKIIRGAKPGELPVQQATEFELTINLKAAAELGLKISPSLIARADEVIE"
     gene            complement(4991..>5954)
                     /locus_tag="VFL49_01475"
     CDS             complement(4991..>5954)
                     /locus_tag="VFL49_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7910676.1"
                     /translation="PDFPEPHWNTASLRLLTGDFSRGWAEYEWRWKKESMARRMRNFS
                     QPLWLGGDDIAGRTILLHSERGFGDTIQFCRYVPLAAARGACVILEVEKPLQRLMTTL
                     AGAAQVVTRAGPLPEFDLQCPLLSLPFAFQTELGTIPWSGPYLRPQSRELTAWDVRLG
                     GKRRPRIGLAWSGSPAHERDAFRSINLCALLPLLNIDATFVSLQKDVRPADAAVLDQC
                     GDVLLFASALNDFSDTAALISKLDLVISVDTSIVHLAGALGRPVWVLLPYIPDWRWLL
                     DRDSTPWYPSARLFRQDDTRTWDNVINRVRDAALKFVESGAAES"
BASE COUNT         1253 a         1750 c         1733 g         1218 t
ORIGIN      
        1 ggcaatccgg tggccgggcc gtaggcgtac tcgcgcttcg cgtcgtcgtg gaggacgagc
       61 atcatgagcc gcgccccgtc tcccgcaccc gtgtactcga gcatctcctg gtcgccggtc
      121 gagttgccga acgccgcgta cggccgccgg ccgatcatca agtggatgcc ttcgggcttt
      181 ccggcgttgt cgtcattgag aagcagcttc ggctctttcg tgaggaacgg cgtgccgtct
      241 ttgtgatatc cgaacttcgt gccgccgacg gtaccgacca cctgctcaac gggtatgccg
      301 taaacctgat tgctgtagac acggacgaaa tcctggccgc cgccagtcac gatgtatgtc
      361 ttgaagccgt tactgcgtag atactgcatg acttcgagca ttggctggta aatgagttcg
      421 gtgtaggggc gcttccaacg ttcgtgtttg gcgcttgtca gccatttctt gacttcggcc
      481 tcaaagtcat caaccgacat gccggtcagt gtcgcggcaa gaattttctc aaggtcatgg
      541 agtgggagtt tggcgattgc ctcgcggtta ccggagagaa ccgtcttgaa tggttcgacg
      601 ttcttgagtt tcgggtccgt ctccaccacg gcaggtacgc ggtccagaca atagacaacc
      661 tgcgaataga tcgggtgttc gacccagagt gtgccgtcct ggtcgaaggt ggcgatacga
      721 tcttccggtg gcaggaattt tggacttgcc tggtcagtgg ttgcccgcac gaagtcgagg
      781 atcgcctgct tcgccgctcc gtcgttccac gaggcgagcg gcgcgcccga tgttgtttgc
      841 gccagggccg aagtggaaaa aagcgcctca gacgatgcag gaagcaaggc tatacttgaa
      901 atcactgcgc ggcggttgat gtcggatggc gaatgatcgg atttcattct cgacctccta
      961 tgtcagcatc ccggccgccc cttcacagct cccagctctt gagcaacttc cgcgccgtgc
     1021 caaaattgcc gagatgataa acagacgcct tgattggcga gaacgtaaaa gcctcgaact
     1081 tctcgtcgag catcttcttt aggtagtcct gcgaggcgag gccaatagtc acgtgcgggt
     1141 tgaacttttt gcccgtttca ttcggaacga aattcgccac gtaatctatg gtcggttgat
     1201 tgatctcggg gtcttccttt gtcgtaacga acgccgatgc agtgcccgtc tccaccgtga
     1261 atggggtgac gacgtcgatc agtttctgtt gatagcggat aaggtcgtcg gtgggctcga
     1321 caacaatccc ggcaaggccg atatttttcc aggggataaa atagtacttg tacgccttca
     1381 gcttccaggt ggccggcttc tccccggcca gcactttgcc aactgcgtcg tagactttgt
     1441 tcaggtcctg cgttttgaca taccgctgca gaaccgagat gtgtggttga tgctccttac
     1501 ccaacgcaaa gccttcgggg aagctcctgc gcagacgctc attggcggcc accgcgtgct
     1561 ggatcatcgt agcgtcggga tcgagcagag tgtcaatcgc ggtgactggc tcgccagcgc
     1621 caaccggctg cacactaccc ggctgcgcaa gcacgggaga tttataaagg accgctccag
     1681 cgactgaacc aagcaatgct gtcaatacgg cgcgcctgtc atttttgcgc atggcgaact
     1741 ccgatcagcg acagggtgaa gtcacccacg cggatattga cagcgggcca gtgcgagtaa
     1801 attgccccta ggggcaacaa ctgtttttgt tttatgtctt ttttatttcg tccggtcaga
     1861 agcggacatc ggccgtctca ttcaatcacg ctaagtttgc gcggtccttg gtgcttggac
     1921 tgcaactgtc gctgtccgcg gcatcaacac tgcaagtatg atcgcgacaa ggataagcgc
     1981 cggcgcgtct cccactaaat gaccgcgctc agccgcatct gtaaggccct ggacgcccat
     2041 gatgatccca tgaacgacgc tcgaccacac ggtgaaccaa ataagacttt tgttcgcgta
     2101 ggggtctcgc gatgcaatga gtagaaatac gccgagcgtt gcgtacacgc caataatcat
     2161 cagcagatag tgtgaatgtc cgtgacccca cgtccagccg gaagaccaca caatggtcaa
     2221 tggataaaag ccaaatatgg caaaaaggcc gatgacgacg agcgcgatgc ggagaagttt
     2281 caagcgactt tcatcagaca tgtttgtgtc tccttttcaa taattccctg aggcgtcgct
     2341 ttttggccgg ctaggcgccg ctgcaggacc cgattgagct cgctcaccgg tttctgcgct
     2401 gtacgctccg ccactagcgc ccgcgcgaga tttactttcc gcgcgcgaaa ggctgcctcg
     2461 gtaaaggtga gatggatcag gtcgcactgg gcgaggctgc cgccgcaacg atgcgcaaga
     2521 tggcgcacgc gtgccagcgt ttttacggcg ctcgcgtaat cactgtgagc gaacgcgagc
     2581 aacgccttgc agagctgcag caacagcgca tcctcggaca tggcgccgct cgtctcgaca
     2641 tgaggcagcg catcgaagat gcgttgcact tccgcagcac gtcctgcggc ggcgagtgcc
     2701 atcatcgcat gcgcgacata gaagggccgc acgccagtga ggtcatgctt ctgccagcgg
     2761 tccgccaaca gcatccagcg ttcgcccacg cggaaattca ataggtccag gcgccacagc
     2821 aacgcagacg catcggcgag ctccgacata cccggttcgc gcgtgttctc gatctgagca
     2881 tcataaaccg cgagcgcaga cttcgggtcg tccgcgtcga gatgaaacag ggcacgatgc
     2941 caggcgagat ggacggaaat gccggttgtg tctttccata ccggctcggt ctccgcaaga
     3001 aattcgagcc cctcttgatc acgcccttgc atttccatca catgggcgat gacatgaatg
     3061 acgctggggt gtcgcgggtc caaggcgaac gcgcgccgtg cgatctcctc ggcacgacga
     3121 tactggccat tctcttcgag accgaaggcg tacatcgcaa gaacgcttgc gtagccggga
     3181 agcgtttcgt tccattcggg catcacctgg gcgacgcgat ctcgcagcat gcgtcgctgg
     3241 ccgagccgaa aatcaagggt atgagcaacc gcaagcgcca gaatgtcgcg cggatggtcg
     3301 atcaggattg caccataacg ttcaactgcg agagcctggt cgccttcgag ccaggcacgc
     3361 gccgccgacg cgtggcggcg tgcagggtca tttatgtccg gacaggtcgc ttcaatggcg
     3421 gtcacgctcg caacaagttt tgttcgcgcg gcatcgtgat cgccccgtaa aaggatagtt
     3481 gcacgcacac aatgggcaaa aatgaattgt ggatcatctg caagtacgcc gtccacctcg
     3541 gcaagaggat tgccgcggct tgtgagcatg agctcaagcg cgcggttgca gcgcgcatgt
     3601 gaatcaaaac cggctgtggc gggctctgcg tttttggcgg gcgtcataaa aattaccttt
     3661 ccgctcgccc aacccgatgc cacaacggaa ggcaaaagac gttgccaaga gcttgattta
     3721 cgaccgagga acgcttgatt atttcctgag gagatcgtgt tggccgacgt ccgcagtgca
     3781 caagctgatg tctgccgtgc cccgaaaggc gaccgaaata gcggacatgg gctgtttccc
     3841 cgcacgcgcc cgctttccaa gccagagcag acatcgtgcc cccgcaccca aaagccgagg
     3901 ttcgacaatc gcggcccttc cagaggggtg ttctggatgg ctacactggc gcgatatgaa
     3961 gaccgggggg tcggtgaaac ggcgaaaatt cctcgcgctt cttgccggcg cggccgcaac
     4021 ctggccggtc tcgtcgcgcg cgcagcagct tccgaaattg acgaccgtcg gcgtcctcgc
     4081 gagtcaactg ctccctcccg tccggaggct cacacccaga ctgcaagagc tgggctacac
     4141 cgagggtcaa aatctccgca tcgaataccg ttttgctgaa ggccatgaca accgctatcc
     4201 gagtttggcc gccgaactgg ctgcgcttcc ggtcgatctg atcgtcacgt ccggcacccc
     4261 cgcagcgctt gccgcgaagc gcgcaaccag caccattccc atcgttatgg cgacaatcgg
     4321 cgaagcagta agcaccggag tggtctcaaa cctcgcgcgt ccgggtggaa atatcaccgg
     4381 tttcaccgca ttgaatctgg agctcgaagg caaacgactg gcactgctca aggagcttct
     4441 gccgcatctt tcgcgcgtcg gcatactggc caacagggca aacccgctgt tcgacgtctc
     4501 tttgcgcaat cttcggccgg cggcgaacgc cctgggtttg acgctcgata ttttcaacgt
     4561 tcgcgataag gacgatattg aaagcgcctt gctgcgaatt gaccatgctc atccggatgg
     4621 ggtcgttgtc gctgccgaca cattgctgct gagcaagcga ggcgagatag cggcatccct
     4681 ggctaaaggt cggtttccgg cgatctatgc gtttcgagag tacgcagacg tcggcggcct
     4741 gatgatctac ggcgcaaatc tcgggatcct gttcgagcgc accgcagatt acgtcgacaa
     4801 gattatcagg ggcgcgaaac ccggcgaact gccggtccag caggcaacag agttcgagct
     4861 gactatcaac ctcaaagccg ccgccgaact tggtctgaaa atctcgccgt cgctcattgc
     4921 ccgcgctgac gaggtgatcg aataaacacg atgccgctcc gggtggaatt gcggcctgca
     4981 atggctacga ttaactctct gcagctccgc tttcgacgaa tttgagcgct gcgtcgcgga
     5041 cgcggttgat cacattgtcc caggtacgcg tatcgtcttg ccgaaacaag cgcgcgctcg
     5101 gataccacgg tgtggaatcc cggtcgagca gccagcgcca atccggaatg taaggcagca
     5161 agacccagac cggacgtccg agcgccccgg cgagatgaac aatgctggta tccaccgaga
     5221 tgacgagatc cagtttcgat atgagcgctg ccgtatcgga gaaatcgttc agcgcgctgg
     5281 cgaaaagtaa aacgtcgccg cattgatcaa gtaccgccgc atcggcggga cgaacatcct
     5341 tctgcaggct cacaaaagtc gcatcgatgt ttaaaagcgg cagaagtgcg cacaggttga
     5401 tcgaccgaaa ggcatccctt tcgtgcgccg ggctgcccga ccaggcaagt ccaatcctcg
     5461 gacggcgttt tcccccaaga cgcacgtccc aggcagttaa ttcccgtgat tgcgggcgca
     5521 gatagggccc gctccacggg atcgtcccaa gctcggtctg gaatgcgaac ggaaggctaa
     5581 gcaagggaca ctgcaaatcg aattcaggca aggggccggc cctggtgacg acctgcgccg
     5641 ctccggcaag tgtagtcata agccgctgaa gcggtttttc gacctcaaga atcacgcagg
     5701 cgccccgcgc cgcagccaat ggtacatagc ggcagaattg aattgtatcg ccaaatcccc
     5761 gctcgctgtg cagcaggatt gtcctgccgg cgatatcgtc gccgccaagc cagagtggct
     5821 gggagaaatt ccgcatacgg cgagccatcg actccttttt ccagcgccac tcatattcgg
     5881 cccagccacg actgaaatcg cccgtgagca ggcgcagcga agctgtattc cagtggggct
     5941 cgggaaagtc cggc
//
LOCUS       DASSRL010000071         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_386896, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000071 DASSRL010000000
VERSION     DASSRL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386896"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VFL49_01480"
     CDS             complement(<1..169)
                     /locus_tag="VFL49_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7910677.1"
                     /translation="MTRASAVTAMVNTPADAMLHALPHPVIMVSADGKIADANVAAEQ
                     FFEASTAMLRRH"
     gene            complement(166..1185)
                     /gene="dusB"
                     /locus_tag="VFL49_01485"
     CDS             complement(166..1185)
                     /gene="dusB"
                     /locus_tag="VFL49_01485"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7910678.1"
                     /translation="MFEPIGLDIGSAKARNRVILAPMSGITDAPFRRLAERLGAGLVV
                     SEMTAGAGLVRGDREAMVRSDGQGVAMHVVQLAGCEAHWMAEGARIAEAQGADIIDIN
                     MGCPSKQVTNGASGSALMRNLDHALGLIEATVSAVSVPVTLKMRLGWDASNMNAPDLA
                     RRAEAAGIKLITVHGRTRCQFYNGIADWGAVRNVKRAVSIPVVVNGDIGSCEDAIEAL
                     KISGADAVMIGRAAQGRPWLPGQIARYLTTGTREQALPLAEQHSLIDDLYGEMLSHHG
                     LAIGRRHARKHLGWALDHAAATAGTPFEVLKSHRSRVLTAEEPAHARRYLAEAFDDFG
                     WRAAA"
     gene            1374..2543
                     /locus_tag="VFL49_01490"
     CDS             1374..2543
                     /locus_tag="VFL49_01490"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HET7910679.1"
                     /translation="MSQVAAVVVAAGQGLRAGGDMPKQFREIGGASLLRRSLLGFAGV
                     STISALQPVVRRDDIDLVREHTRDIRILPPVFGGKTRQASVRAGLEALASRSPDIVLV
                     HDAARPFASADLIARAISAAEKNGAAIPVLPVTDTVKRLGPDGTIEKTIDRGPLRLVQ
                     TPQAFAFDALLEAHRRAAEKGRDDFTDDAALAEWAGMTVTVFAGEPGNIKLTTPEDFA
                     RAEAAQSAALGDVRTGSGLDVHAFGPGDHVVLGGVRIPHTHALTGHSDADVALHALTD
                     AILGAIAEGDIGAHFPPSDPQWRGASSDRFLAFAVDRVRARKGRIAHLDLTIVCEAPR
                     IGAHRDAMRANIARLAGIGGERVGVKATTSEKLGFTGRGEGIAAYATATIRLPWE"
     gene            2545..3069
                     /locus_tag="VFL49_01495"
     CDS             2545..3069
                     /locus_tag="VFL49_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET7910680.1"
                     /translation="MAEQSAIDAAKALLDLCTRKKLTLATAESCTGGLVAATISEIPG
                     SSAVLDRGFVTYSNEAKQQMLGVAPATIDVYGAVSTECAEEMAKGALAHALVDLAVSI
                     TGIAGPTGAVPGKPIGLVYFCAASRSGRVIAHDRKYGDIGRTKVRGQSVLQALSMLQE
                     LAAKEEPRPPAGES"
     gene            3201..4289
                     /locus_tag="VFL49_01500"
     CDS             3201..4289
                     /locus_tag="VFL49_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET7910681.1"
                     /translation="MKRRQFLQAAGVGLAASAVTAPAIAQSSPEVKWRLTASWPKSLD
                     TLYGGCEYFSKRVAEITDNKFQIQVFAAGEIVPGLQVLDAVGNGTVEIGNTALYYYFG
                     KNPALTFGTALPFGLNARQMISWLRHGGGEELLNNLLKDFNCVGFAAANTGAQMGGWF
                     RKEIKSTDDLRGLKMRIGGFAGTIVAKLGLVPQQLAAGDIYPALEKGTLDACEWVGPY
                     DDEKLGFVKVAKYYYYPGWWEGCGQAHNIMNLAKWNELPKAYQSAIITASNDAWIWTL
                     SKYDYVNPIALRRLISSGAQLRPFPQPVMEACYKAANEIYDGLAKNNPHFKKLYDSLV
                     PYRSDSFAWLQVAELGFDSFMMRMRTRS"
     gene            complement(4342..>4509)
                     /locus_tag="VFL49_01505"
     CDS             complement(4342..>4509)
                     /locus_tag="VFL49_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7910682.1"
                     /translation="HADGENACEVEFFITYEFKSRALAMLMGAMFDTAFRRFAVAFER
                     RADAVYGRKMA"
BASE COUNT          902 a         1401 c         1338 g          868 t
ORIGIN      
        1 ggtgccgacg cagcatagcc gtcgaagctt caaaaaactg ctctgcggcc acgttcgcat
       61 ccgcaatctt cccgtcggca gagaccatga tcacggggtg cggcagggcg tgcagcatcg
      121 cgtccgcagg cgtgttcacc atcgcagtga ctgccgatgc tcgagtcatg cggcagctct
      181 ccagccgaaa tcgtcgaaag cttcggcgag ataacggcgc gcatgcgctg gctcctcggc
      241 ggtgagcacg cggctgcggt gactcttgag aacttcgaat ggggttccgg ccgttgccgc
      301 cgcgtgatca agcgcccagc cgaggtgttt gcgcgcgtgg cggcgcccga tcgccaggcc
      361 gtgatggctc aacatctcgc cgtacaggtc atcgatcagg ctatgctgct ccgccaatgg
      421 cagcgcttgt tcgcgcgtgc ccgtggtcag gtatcgcgca atctgcccgg gcagccatgg
      481 acggccttgc gcagcacgcc cgatcatgac cgcgtcggcg cccgagattt tcagggcctc
      541 gattgcgtcc tcgcaagatc cgatgtctcc gttgacgaca acgggaattg acaccgcccg
      601 cttgacgttg cgaacggcgc cccaatcagc aataccgttg tagaactggc aacgggtgcg
      661 tccatggacg gtgatgagtt tgatccccgc cgcctcggca cgtcgcgcaa gatcgggcgc
      721 gttcatgttc gacgcatccc agcccaaccg catcttcaag gtcactggaa cactgactgc
      781 ggaaaccgtc gcctcgatca agccgagcgc atgatcgagg ttccgcatga gcgcggagcc
      841 cgaagctccg ttggtgacct gttttgacgg gcaccccatg ttgatatcga tgatgtcggc
      901 gccctgcgct tcggcaatcc gggcaccctc ggccatccaa tgcgcctcgc agccggcaag
      961 ctggacaacg tgcatggcga cgccctgccc gtcgctccgc accatcgcct ctcggtcgcc
     1021 gcggacgagc ccagctcccg cggtcatttc cgacacgacc aagccggcgc caagtcgttc
     1081 ggccagacgg cgaaatggcg cgtcggtaat tcccgacata ggagccaaaa tcacacggtt
     1141 gcgcgccttg gccgacccga tatccaggcc gatcggctca aacatgatat cgttcaatgt
     1201 attagccatt atgcctatca tttaggcgat agcttttgat gcacagagtt tagccaatta
     1261 cacccacaaa gcaagtgctg cactgcaaaa aactgttcat gcgcatgcat caaaaactgc
     1321 tgcgtttgcg gaacggaaat aacgcggtta aggcaacatc tttggagacg cagatgtcgc
     1381 aagtcgcagc tgttgtggtg gccgcgggcc agggcctccg cgcgggcggc gacatgccca
     1441 agcaattccg cgaaatcggc ggggcgtccc tgctccgccg ttcgctgctg ggctttgccg
     1501 gcgtctcgac tatcagcgcg ttacagcccg tcgtccgcag ggacgatatc gatctcgtgc
     1561 gcgaacacac gcgcgacatc cgaattttgc ctccggtttt tggcggcaag acgcggcagg
     1621 cctcggtgcg cgcaggcctt gaagcccttg cttcgcgttc gcccgatatc gtccttgtcc
     1681 acgatgccgc gcggccattt gccagcgccg acctgatcgc acgcgcaatc tccgctgccg
     1741 aaaaaaacgg tgcagcgatc ccggtgctgc cggtgaccga taccgtcaaa cgtctgggac
     1801 cggacggcac tatcgagaaa acgatcgacc ggggcccgct tcgcctcgtg cagacgccgc
     1861 aagccttcgc tttcgacgca ctccttgagg cccaccggcg cgcggctgaa aagggccgcg
     1921 acgactttac cgacgacgct gcgctggccg aatgggctgg tatgactgtg acagtgttcg
     1981 ccggcgagcc gggcaatatc aagctcacca cgcccgagga cttcgcgcgt gctgaggcgg
     2041 cacaatcggc ggcactcggc gacgtgcgga ccggttccgg ccttgacgtc cacgcatttg
     2101 gccccggcga ccatgtcgtg cttggaggag tccgcattcc gcacacgcat gcgctcaccg
     2161 gtcactccga tgcggatgtc gccttgcatg cgcttacaga tgcaattctc ggcgcaatcg
     2221 ctgaaggcga cataggagcc catttccccc cgagcgaccc gcaatggcgt ggcgcctcgt
     2281 ccgaccgctt ccttgccttt gctgtagacc gcgtgcgggc aaggaaaggc cgcattgccc
     2341 acctcgatct cacgattgta tgcgaggccc ctcgcatcgg cgcgcaccgc gacgcgatgc
     2401 gggcgaacat cgccaggctt gccggcatcg gcggcgagcg cgtcggtgtc aaagcaacca
     2461 caagcgagaa gctcggcttt accggacgcg gcgaaggtat agcggcctac gccacggcaa
     2521 cgatccgtct gccttgggaa tagtatggcc gagcaatccg caatagatgc cgcgaaggcg
     2581 ctgctcgatc tctgcacacg gaagaaattg acgcttgcaa ccgcggaatc ctgtaccggc
     2641 ggccttgttg cggcaacgat cagcgaaatt cccggctcgt cggcagtgct cgatcgcggc
     2701 ttcgttacct attccaatga ggccaagcag cagatgctgg gcgtcgctcc tgcgaccatc
     2761 gatgtctatg gcgcagtcag tacagaatgc gctgaagaga tggccaaagg tgccctggcc
     2821 catgcgttgg tcgatctggc tgtttcgatt accggcattg ccggaccaac cggcgcagtg
     2881 cccggcaagc cgatcggcct ggtctacttc tgcgcagcat cgcgcagcgg ccgcgtcatt
     2941 gctcacgacc gcaaatacgg tgacattggc cgcacaaaag tgcgcgggca gtccgtgctt
     3001 caggcactga gcatgctgca agagcttgcg gcaaaggaag agcctcggcc gccggcgggc
     3061 gaaagttaat tcaagtccgg cctttttctt cgcacggaat ggcgtaggat tgagtatcgg
     3121 ccgccggcaa ggggcgagct tcagacattc atgagaaatc tcctccggtc ggcggaccga
     3181 agtcaacgag gggaaacgtc atgaaacgtc gtcaattcct gcaagccgca ggcgtggggc
     3241 ttgcggcgtc ggccgttact gcacctgcaa tcgcccaatc atctccggaa gtgaaatggc
     3301 gtctgacggc cagctggccg aagtcgctcg atacgcttta cggcggatgc gaatattttt
     3361 cgaagcgcgt cgccgaaatc accgacaata aatttcagat ccaggtattt gccgccggcg
     3421 aaatcgttcc cggcctgcaa gtcctcgatg cagtcggcaa cggcactgtc gagattggca
     3481 atacggcgct gtactactat ttcggaaaga acccggccct cacattcgga actgcgctgc
     3541 cattcggatt gaatgcccgg cagatgattt cctggctgcg ccatggcggc ggcgaggaac
     3601 tgctcaataa tctgctcaag gacttcaatt gtgttggttt cgccgcggcc aataccggcg
     3661 cgcagatggg cggctggttt cgcaaggaga tcaaatcgac cgatgacctc agaggtttga
     3721 agatgcgcat cggcggtttc gccggcacta ttgtggcgaa actcggattg gtgccgcagc
     3781 agctcgcagc cggcgatatc tatcccgcgc ttgagaaggg aacgctggac gcctgcgaat
     3841 gggtaggccc atatgacgac gaaaagctcg gcttcgtgaa ggtcgccaaa tattactact
     3901 accccggctg gtgggagggc tgcggccaag cccataacat tatgaacctg gccaaatgga
     3961 acgagctgcc caaggcgtat caatcagcca tcatcacagc gtcgaatgac gcctggatct
     4021 ggacattgtc gaaatacgac tacgtcaatc cgatcgcgtt gcgtcgactg atttcgagcg
     4081 gggcccagct gcggcccttc ccgcagcccg ttatggaggc ctgctacaag gccgcaaacg
     4141 agatttatga cgggctcgca aaaaacaatc cgcacttcaa gaagctatat gacagccttg
     4201 tgccgtatcg ctccgactcg tttgcctggt tgcaggttgc cgaactcgga ttcgacagct
     4261 tcatgatgcg gatgcgtacg cggagctaaa actcgcgccg cattcgtggt ccggcgggca
     4321 tcgctcgccg gaccatttta tttacgccat cttgcgtcca tagacggcat cggcacgccg
     4381 ttcgaaggcg accgcaaagc ggcggaatgc cgtatcaaac atcgcaccca tcagcattgc
     4441 gagcgcgcga cttttgaatt catacgttat gaagaattca acctcgcagg cattttcccc
     4501 gtctgcatg
//
LOCUS       DASSRL010000072         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1104271, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000072 DASSRL010000000
VERSION     DASSRL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104271"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFL49_01510"
     CDS             complement(<1..606)
                     /locus_tag="VFL49_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HET7910683.1"
                     /translation="MAAALMVALLAIAPAFAINFPALTGRVVDQANIIQSDRRVAIEQ
                     KLTDLETKSGIQLVVATVNSLEGQDIEPYANELFRKWALGEKKKNNGVLLLVAPNERR
                     VRIEVGYGLEGTLTDALSKVIITNAMTPRFKAGNFSDGISRGVDDVITVLTTDASEWQ
                     QRPSLRLDYQQPVDPANWILLAALFVFFILLIVSPTFRWLFF"
     gene            complement(677..1276)
                     /locus_tag="VFL49_01515"
     CDS             complement(677..1276)
                     /locus_tag="VFL49_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7910684.1"
                     /translation="MIVSVAGIRAFLAVTLLGLTVAGCGYNTIPTLEEQAKAKWSDVQ
                     NNYQRRADLIPNLVATVQGYAKQEREVLTSVVEARAKATSVKVDASQLTDPEKIKQFQ
                     DAQNQLTGALGRLIAISENYPDLKSNQNFLALQSQLEGTENRIAVARRDYIEAVRAYN
                     TELRTFPGVIWASTFFRSNKPMAEFTASGDSAKPPQVKF"
     gene            complement(1402..2460)
                     /locus_tag="VFL49_01520"
     CDS             complement(1402..2460)
                     /locus_tag="VFL49_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7910685.1"
                     /translation="MTRQITKRDVLKGAGAIGLSAFAVRVAAAPAAEAVTPALIAAAK
                     KEGKITFYTAMDLQFAERLGKAFEAKYPGIAARVERSGAERVFTRIAQEYSSNIHGVD
                     VVNTSDQAHCIIWKRNGWLAPYLPEEVVKHYDKRYYDPEGLDVVTRILISPIAYNTNL
                     VKEEDAPKSFRDLLDPKWKGKLVKAHPAYSGTIMNSTFEVARDLGWDYFVKLAAQNVM
                     QVQSATDTPKRISLGERAVMVDGAGYLVIRYKESGQPVDIIYPEEGTPLATGPSAVFK
                     AAPNPNAARLFQNWMHSREAQQILVDYARQYSPHSQTVEKPGIRRLTDIKLMKEDPEG
                     ILAQGEAVKKRYAEIFKV"
     gene            complement(2495..>3266)
                     /locus_tag="VFL49_01525"
     CDS             complement(2495..>3266)
                     /locus_tag="VFL49_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910686.1"
                     /translation="TTPERARVHAAELVKLKPDVIVSHATIVTRAFFQGTRTIPVVFT
                     NVSDPLGEKFVQSFAKPGGNITGFTNIESTMGAKYLQLLKDIAPGVTRAAMLFNPKSA
                     PGGGSYFTDAFEAAAPQFSIQAIKATVQDEAGIEKAISTLASGGGGGLIVVGEPFTNA
                     HRSKILELTSRYRLPTICPYRFYAANGCLLSYGVDLSDQFKRAATYVDRILKGASATS
                     LPVQSPVKFELAVNVKTAKALGLTISPTLLARADEVIE"
BASE COUNT          644 a          955 c          955 g          712 t
ORIGIN      
        1 gaaaaacagc caacgaaagg tcggcgacac aatgagcagg atgaagaata cgaacagcgc
       61 tgcaagcaaa atccagttcg cgggatcgac cggttgctga tagtcgagcc gcaatgaggg
      121 gcgctgctgc cattccgacg cgtcggtggt caataccgtg atgacgtcgt ccacgccgcg
      181 cgaaattccg tcgctgaaat tgccggcctt gaagcgcggc gtcattgcgt tggttatgat
      241 gactttcgac agcgcgtcgg tcagcgtccc ctcaagtcca tagccgacct cgatgcgaac
      301 ccgccgctcg ttcggcgcaa ccagcagcaa aacgccgttg ttctttttct tttcgccgag
      361 cgcccacttg cggaagagct cattggcgta aggctcgatg tcctggcctt ccagtgaatt
      421 cacggttgca acgacgagct ggatcccaga ctttgtttcg agatcggtca gcttctgctc
      481 aatcgccacc cgcctgtcgg actggatgat gttggcctga tcgacgacac ggccggtgag
      541 cgcagggaaa ttgatggcga aagcaggcgc gatcgcaagg agcgccacca tcaaggccgc
      601 ggccatacgc tttcttccgc cggtggcaga aaaagtgccg gccgcgttca tattgatgcc
      661 ttcacaccgc agggcgctag aactttactt gcggcggctt ggccgagtcg ccgctggcag
      721 tgaactccgc catcggcttg ttggaccgga agaatgtcga ggcccagatc acgccaggga
      781 atgttctgag ctcggtatta taggcccgca cagcctcaat atagtcgcgg cgcgcaaccg
      841 ctatgcggtt ctcggtgcct tcgagctgag actggagcgc aaggaagttc tgatttgact
      901 tgaggtcggg atagttctcg ctgattgcaa tcaatcggcc gagggcgccg gtaagctggt
      961 tctgtgcatc ctggaactgt ttgatttttt cgggatcggt aagctgcgaa gcgtctacct
     1021 tcacgctggt cgctttcgcg cgcgcctcaa cgaccgaagt cagcacttct ctttcctgct
     1081 tggcgtaacc ttggactgtc gcaacaagat ttggaatcag gtcggcgcga cgctggtagt
     1141 tgttttgcac gtccgaccac ttggctttgg cctgctcctc cagagtcggg atcgtgttgt
     1201 agccgcagcc ggcgacagta agtccgagca acgtgacagc aagaaaggcg cgaatacctg
     1261 ccaccgatac gatcatgacg acctccggtc gaattgtgtt tcagaacgcc gattatccga
     1321 aactggagcg ggaagacgaa ttggaccagg gtccaacgcc caggcaagca gaacgttgcc
     1381 aggccggccg gagcagccgc atcacacctt gaagatctcc gcgtagcgtt tcttcaccgc
     1441 ttccccctgc gccaggatgc cttccggatc ctccttcatc agcttgatgt cggtgagcct
     1501 gcggatgccg ggcttttcca cggtctgcga gtgcggcgaa tactggcggg cgtagtcgac
     1561 gaggatctgc tgggcctcgc gcgaatgcat ccagttctgg aatagacgcg cggcattcgg
     1621 attgggagca gccttaaaga ccgcacttgg accggtcgca agcggcgtgc cctcctccgg
     1681 ataaattatg tcgaccggct gacctgattc cttgtagcgg atgacgaggt agccggcgcc
     1741 gtccaccatg accgcacgct cgccgagcga aattcgcttc ggcgtatcgg tcgccgactg
     1801 gacctgcatg acgttctgcg ccgccagctt cacgaaatag tcccaaccga gatcgcgcgc
     1861 gacctcgaac gtcgagttca tgatggtgcc gctgtaggcc gggtgtgcct tgaccagctt
     1921 gcctttccat ttcggatcga gcagatccct gaagctcttc ggtgcgtcct cctccttcac
     1981 caggttggtg ttgtaggcga tgggagaaat gaggatgcgc gttacgacgt ccaacccttc
     2041 gggatcgtag tagcgcttgt cgtaatgctt cacgacctct tccggcagat acggcgcgag
     2101 ccagccattg cgtttccaga taatgcaatg cgcctggtcc gaggtgttga cgacatcgac
     2161 gccatgaatg ttggaggaat attcctgggc gatgcgggta aacacgcgct cggcgcccga
     2221 ccgctccacg cgcgcagcaa tgcccggata cttcgcttcg aatgccttgc cgagacgttc
     2281 cgcgaattgc agatccatcg ccgtgtaaaa agtgatcttg ccttcctttt ttgcagccgc
     2341 gatcagcgcc ggcgtgaccg cctccgctgc gggcgccgca gcgacccgca cggcaaatgc
     2401 gctgagacca atcgctccgg cacccttgag cacgtcgcgt ttggtgattt gtctggtcat
     2461 ggcgtcctcc atcttttttg ctccggccag tttcttattc gatcacctcg tcggcacgag
     2521 ccagcaaggt cggcgatatg gtgagtccca gcgccttggc tgttttgaca ttgactgcga
     2581 gctcaaattt taccggcgac tgcactggca gactggttgc gctggcacct ttgagaatgc
     2641 gatcaacgta ggtcgccgca cgcttgaact ggtccgacag atccacaccg tacgacagca
     2701 ggcatccatt ggcggcgtaa aagcgataag gacaaattgt tggcagccga taccgcgacg
     2761 tcaactcgag tattttggag cggtgcgcat tcgtgaaggg ttcccccaca acaataaggc
     2821 cgcccccacc gccgcttgcg agcgtcgaaa ttgccttttc gatacctgcc tcgtcctgaa
     2881 ctgtcgcttt gattgcctgt atcgagaatt gcggggcggc ggcctcgaac gcatcagtga
     2941 aatatgaacc gccgccgggc gccgacttcg gattgaaaag catcgcggca cgcgtgacgc
     3001 caggcgcgat atccttgagc agctgcagat atttcgcgcc catggtcgat tcgatatttg
     3061 tgaatccggt tatgttgccg cccggcttcg caaaactttg aacgaatttt tcgccgagag
     3121 gatcggaaac gttggtgaac acgaccggga tcgtcctggt cccctggaag aacgcgcgcg
     3181 tgacgatggt cgcatgcgag acgatcacat ccggtttcag cttgaccaat tccgctgcat
     3241 gaacccgcgc ccgttcgggg gtcgtt
//
LOCUS       DASSRL010000073         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_596902, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000073 DASSRL010000000
VERSION     DASSRL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596902"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFL49_01530"
     CDS             <1..260
                     /locus_tag="VFL49_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910687.1"
                     /translation="NKAKTLESVKVAKALEGLELPPEVKLQPNKVYFRPGDHQLMSSE
                     FPGEIKQDGKYPDVFKVADIVAGDKIALSVKDAGCHLDYKA"
     gene            359..1228
                     /locus_tag="VFL49_01535"
     CDS             359..1228
                     /locus_tag="VFL49_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7910688.1"
                     /translation="MELLLFNAFNGLIQGVFYALMALGLALILSLNSVINMAHGGFLV
                     IGAYLAYTLSPYLGFWGALVAGPVLAGAIGLVVERVLIRPIYKRPDPLYSLLLTFGLA
                     LIIEDLTRTIWGPQGLPLEVPDIINAPVSETYFFVTGYRLFVMAITAAAVIGLYLLMR
                     YTLLGIRIRAGTRDLETTSALGVNIYKLRALNFGFGCIFAGLAGVLAAGVLGLTPPMG
                     NSLIMPSFVAIIIGGVGSLFGAVLGGLIIGLAAGITTTYYPAASEAVIYLMMLIVLVV
                     RPRGLFGEEGLYE"
     gene            1225..2169
                     /locus_tag="VFL49_01540"
     CDS             1225..2169
                     /locus_tag="VFL49_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7910689.1"
                     /translation="MTRIVGIVAMWVALVAAPYWLPLVGSYTALGSKVLIYAIATMGL
                     NIVLGFTGGLSFGQAAYFGLGAYGAGMTLKYLAPSTPLAILVGTLAGGIAATLLGPLV
                     MRRRGIYFAMVTIAIGQLFYFVMVRWNTVTGGEDGLAGFGRQDIHLGSLVIPIASSGT
                     AFYYLILVCFTIAVALMWMLLQSPLGHTWVAIRENRRRMEFLGIKVERYVWAAFAIAG
                     FFTAFAGTLNALLFNFTSPQDLHWILSGNFVIMIVLGGMRSFFGPLIGVIIFVVAQDY
                     LSSVTSNWMTFIGMIFVLIVLLFPKGVLGIFGRRAEAT"
     gene            2166..>2425
                     /locus_tag="VFL49_01545"
     CDS             2166..>2425
                     /locus_tag="VFL49_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7910690.1"
                     /translation="MTAAAVVSKVRTAARPGTEFLRMEKVSRHFGTLKAVDGVTFDMR
                     AGELRAVIGPNGAGKTTFFNLISGLLPPTHGEVIFENETITQ"
BASE COUNT          400 a          735 c          748 g          542 t
ORIGIN      
        1 cgaacaaggc gaagacactc gagtcggtga aggtcgcgaa agcgctggaa ggccttgagc
       61 tgccgcctga agtcaagctg cagccgaaca aggtctattt ccgtccgggc gatcaccagc
      121 tgatgtcgag cgaattcccc ggcgagatca agcaggacgg aaaatatccc gacgtgttca
      181 aggtcgcgga tattgtcgcc ggcgacaaga tcgcgctctc ggtcaaggac gctggctgtc
      241 acctcgatta caaagcgtga cgcgctagca aaacatattc gcacaggccg cgaccgcgcc
      301 gtccttcaac agggcggcgc ggcgtggcgt caaattgtgt tccagatacg ggcggacgat
      361 ggagcttctg cttttcaacg cattcaacgg gctgatccag ggcgttttct acgcgctgat
      421 ggcgcttggg ctggcgctga tcctgagcct aaactcggtg atcaacatgg cgcatggcgg
      481 atttctcgtc atcggcgcct atctcgccta cacgctttcg ccctatctcg ggttttgggg
      541 cgcgctcgtc gcaggccccg ttcttgccgg cgcgatcggc ctcgtcgtcg agcgagttct
      601 gatccgcccg atctacaagc ggcccgatcc gctttacagc ttgctgctga cctttggcct
      661 cgcgctcatc attgaggatc tgacgcgcac gatctggggg ccgcagggcc tgccgctcga
      721 agtgcccgac atcatcaacg cgccggtgag cgaaacttat ttcttcgtca ccggctaccg
      781 gctgttcgtg atggcgatca cggccgcggc cgtgatcgga ctgtatcttt tgatgcgtta
      841 cacattgctt ggcatccgca tcagggcagg gacgcgcgat ctcgagacga cttcggcgct
      901 cggcgtgaac atctataaac tgcgcgcgct gaatttcggc ttcggctgca ttttcgcggg
      961 gcttgccggc gtactcgccg cgggcgttct cggcctcacg cccccgatgg gcaacagtct
     1021 catcatgccg agcttcgtcg ccatcatcat cggcggagtc ggaagcctgt tcggcgcggt
     1081 gctcggcggc ctcatcatcg ggctcgcggc cggtatcacc actacttatt acccggccgc
     1141 cagcgaagcc gtgatttatc tcatgatgct gattgtgctt gtggtgcggc cgcgcggact
     1201 gttcggggaa gaggggctgt acgaatgaca cgcattgttg gaatcgtggc gatgtgggtc
     1261 gcgctcgtcg ccgctcctta ttggctgccg cttgtgggca gttacacagc actcggttcg
     1321 aaagtcctga tctatgcgat cgcgacgatg gggctgaaca tcgtgctcgg cttcaccggg
     1381 ggcctctcat tcgggcaagc cgcttatttc ggcctcggcg cttatggcgc gggcatgacg
     1441 ctgaaatatc tcgcaccgag cacaccgctt gcgatcctgg tcggcacgct tgcgggtggc
     1501 attgccgcga cgcttctagg cccgctggtg atgcgccggc gcggcattta tttcgcgatg
     1561 gtgacgattg cgatcgggca acttttctat ttcgtcatgg tgcgctggaa caccgtgacg
     1621 ggcggcgagg atggtcttgc cggtttcggg cggcaggaca ttcatctcgg ctcactcgtc
     1681 attccgatcg cgtcttcggg cacggcgttc tattatctca tcctcgtttg tttcacgatc
     1741 gccgtcgccc tcatgtggat gctgctgcaa tcgccgctcg ggcatacctg ggtcgcgatc
     1801 cgcgagaacc gcaggcgcat ggaatttctc ggcatcaagg tcgagcgtta tgtctgggcg
     1861 gcgttcgcaa tcgccggttt cttcaccgcg tttgccggta cgctgaacgc gctcttgttc
     1921 aatttcacct cgccgcagga cctgcactgg atcctgtcgg ggaacttcgt gatcatgatc
     1981 gtgctcggcg gcatgcggag cttcttcggc ccgctgatcg gcgtcatcat cttcgtcgtc
     2041 gcgcaggatt atctttccag cgtcaccagc aactggatga cgttcatcgg catgatcttc
     2101 gtcctgatcg ttctcctgtt cccgaaaggc gtgctcggga tcttcggccg ccgcgcggag
     2161 gccacatgac cgccgcggct gttgtatcga aagtgcggac cgcggcgcgc cccggcacgg
     2221 aattcctgcg catggaaaag gtctcccgcc acttcggcac gctgaaggcg gtggacggcg
     2281 tgacattcga catgcgcgcc ggcgagttgc gggcggtgat cgggccgaac ggtgccggga
     2341 agacgacgtt cttcaacctg atcagcggac tgctgcctcc gacccacggc gaggtgattt
     2401 tcgagaacga aaccatcacc cagca
//
LOCUS       DASSRL010000074         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_561806, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000074 DASSRL010000000
VERSION     DASSRL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561806"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFL49_01550"
     CDS             complement(<1..464)
                     /locus_tag="VFL49_01550"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7910691.1"
                     /translation="MSLLKSVFQPDSFVVADVAPSPNFDARKDNRAPNMIVLHYTGMQ
                     SGETALQRLTAAQSKVSAHYVVFEDGRITQCVLEEWRAWHAGESSWAGETDINSCSIG
                     IEIVNPGHEFGYPDFPLRQIAAVITLCRSILTRRGPINADRILAHSDVAPAR"
     gene            complement(560..1303)
                     /locus_tag="VFL49_01555"
     CDS             complement(560..1303)
                     /locus_tag="VFL49_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7910692.1"
                     /translation="MWVGLLSDIHANREALEACLAHARGNGITHFIFLGDYVGYGADP
                     GFAVDTVMREVEDGATALLGNHDDAIASGTDRMNAVAAEALDWTRSQLNAVQRDFLAG
                     LPLTFEDSDRLFVHASAHAPAEWDYITSIEDASACFSATRAQATFCGHVHVPQLYHLL
                     PSGPLSGIIPAPAIEIPLRVKEKRWIAVIGSVGQPRDGNPAACYGALEMERSVLIYVR
                     VPYDVETAARKIQQAGLPPILGLRLIQGM"
     gene            1565..2044
                     /locus_tag="VFL49_01560"
     CDS             1565..2044
                     /locus_tag="VFL49_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET7910693.1"
                     /translation="MDRFVANFMLRLDSKGRVSIPGAFRAVLARDGVESLYCYPALDR
                     PAIDAGGRTLMADIEAQIGSFAPLSKEREQFAMALYGTSETLKVDGEGRVVLSESLKR
                     HAGITDVAAFIGLGQKFQIWEPGRFQKELAEATQKVRALRGQLRSPMAARNAPGARE"
     gene            2041..>2226
                     /gene="mraW"
                     /locus_tag="VFL49_01565"
     CDS             2041..>2226
                     /gene="mraW"
                     /locus_tag="VFL49_01565"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HET7910694.1"
                     /translation="MTAGSGGSAVAGGPALHIPVLGRQAVGFLDVRNGGVYIDGTFGA
                     GGYTRAILAAADCQVIGI"
BASE COUNT          433 a          652 c          714 g          427 t
ORIGIN      
        1 ttgcgcgccg gcgcaacatc ggaatgggca aggatgcgat cggcattgat cggtccgcgg
       61 cgagtgagga ttgacctgca aagcgtgatg accgccgcga tctgacgcag cggaaaatcc
      121 ggatagccaa actcatgacc cggattgacg atctcgatac cgatcgagca ggagttgatg
      181 tcggtctcgc ctgcccagga tgactcgcct gcgtgccacg cgcgccattc ctcaagcacg
      241 cactgtgtga ttctgccgtc ttcgaaaacg acgtagtgcg cggagacctt gctctgcgcc
      301 gcggtaagcc gctgcaaagc cgtctcgccg ctctgcattc ccgtgtagtg cagcacgatc
      361 atgttgggcg cgcgattgtc cttgcgcgcg tcgaaattcg gcgacggcgc gacatcggcg
      421 acgacgaacg aatccggctg aaaaacggac ttcaagagag acatggaccg gaccgtgccg
      481 tgcgtggctg ccaaaagcgg ggcatcattc accgcggccg gtcaaaaaag caagtttgga
      541 gaaagcaccg gcattgcgac tacatgcctt gaataagacg aagccccaga attggcggca
      601 ggccagcttg ctgaatttta cgggcggctg tttcgacgtc gtaagggacg cgcacataga
      661 tcagaacgct gcgttccatc tcgagcgcgc cgtagcaggc cgccggattt ccatcgcgtg
      721 gctggcccac cgaaccgata accgcgatcc atcttttttc tttcacgcgc agcggaattt
      781 cgattgccgg cgctggaatt attcccgaca gcggtccact tggcagcaag tggtagagct
      841 gcggcacatg cacgtgaccg cagaacgttg cttgcgcgcg cgtcgcggaa aaacaagctg
      901 acgcgtcttc aatgcttgtg atgtagtccc attctgctgg cgcatgtgcg cttgcgtgca
      961 cgaatagccg gtcactgtct tcaaacgtca gcgggaggcc ggcgagaaag tcacgctgca
     1021 cggcgttgag ttgcgaacgc gtccagtcga gggcctcggc agcaaccgca ttcatgcgat
     1081 cggtgcccga tgcaatggca tcgtcatggt tgccgagcaa tgcggtcgcc ccgtcctcga
     1141 cctcgcgcat cacagtgtcg accgcgaagc cgggatcggc accgtaaccc acatagtctc
     1201 cgaggaagat gaaatgcgtg atgccgttgc cgcgggcatg ggcaaggcac gcctccagcg
     1261 cctcgcggtt tgcgtggatg tcgctgagca gtccgaccca catgccgtga cctcacaaag
     1321 gggaattcgg caacgcttct ttaagtttaa ccgccgttaa tggggcccat ctgcgacaac
     1381 gccgtgcttc gcgttggatt ggggccgatt tgttgacggt cacgtaatcc catgatatcc
     1441 catgatttcc cagtacggga cactgtttgc gaacgtgggg ttccggtggt gggcggcccg
     1501 gagagctgga gcgcgcgtgc caggaccgtc ccgaattgcg tccagtatga acgggatacg
     1561 ggatatggac cgcttcgtgg caaatttcat gctgcggctc gactccaagg gacgcgtgtc
     1621 cattcccggg gcatttcgcg cagtgctggc ccgcgatggc gtcgagagcc tttattgcta
     1681 tccggcgctg gaccgtccgg cgatcgatgc gggcggccgt acgttgatgg cagatatcga
     1741 ggcacagatc gggagcttcg caccgctttc gaaagagcgc gagcagttcg ctatggcgct
     1801 ttacggaacg agcgagacgc tcaaagtcga cggagaaggg cgggtggtat tgagcgagag
     1861 cctgaagcgt cacgctggca ttaccgatgt ggcggcattt atcggcctcg gtcagaagtt
     1921 tcagatctgg gaacccgggc gtttccagaa agagctcgca gaggccaccc aaaaggtgcg
     1981 cgcgctcagg ggacagctga ggtccccgat ggccgcgcgc aacgcgcctg gagcacggga
     2041 atgacggcgg gcagcggcgg ctcggctgtc gctggcggac cggccctcca cattcccgtg
     2101 ctcggccgcc aggctgtcgg cttccttgat gtgcgcaatg gcggcgtcta tatcgacggc
     2161 accttcggcg ccggcggcta cacgcgcgcc attctcgcgg cggccgattg ccaggtgatc
     2221 ggtatc
//
LOCUS       DASSRL010000075         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_649550, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000075 DASSRL010000000
VERSION     DASSRL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649550"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VFL49_01570"
     CDS             complement(<1..98)
                     /locus_tag="VFL49_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7910695.1"
                     /translation="MSNRLQGKLALVTAAGQGIGRAIAEAFIVEGA"
     gene            complement(111..1913)
                     /locus_tag="VFL49_01575"
     CDS             complement(111..1913)
                     /locus_tag="VFL49_01575"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HET7910696.1"
                     /translation="MAKTSRKSARRLRSQLWFDNADNPGMTALYLERFLNYGLTSAEL
                     RSGKPFIGIAQTGSDLSPCNRHHLELAKRVRDGIRVAGGIAFEFPCHPIQETGKRPTA
                     ALDRNLAYLGLVEVLYGYPLDGVVLMTGCDKTTPACLMAAATVNTPAIVLSGGPMLNG
                     WWKGERAGSGTAIWKAREEMAAGRIDAEDFIQIAASTAPSIGHCNTMGTASTMNALAE
                     ALGMSLPGCAAIPAPYRERAQIAYETGLRAVEIVEQDLKPSDILTRKAFENAIVANSA
                     IGGSTNAPVHINAIARHVGVKLSIEDWEKIGFDVPLLVNMQPAGEYLGEEYYRAGGLP
                     AVMHELLKAKRIHADVLTINGKTMGENVKRAQSTNADVIKPYRSPMKKHAGFKVLNGN
                     LFDSAIMKTSVISDEFRKRYLSNPKDKDAFEGRAVVFEGPEDYHHRIDDPSLKIDEYT
                     MLFIRGVGPIGYPGGAEVVNMQPPAALIKKGITSLPCIGDGRQSGTSGSPSILNASPE
                     AAANGGLALLKTGDRVRIDLRKGTADILISKAELARRRAALQKRGGFRYPASQTPWQE
                     IQRSMVDQLADGMVLKPAVKYQRVAQNGVPRDNH"
     gene            complement(1991..>3459)
                     /locus_tag="VFL49_01580"
     CDS             complement(1991..>3459)
                     /locus_tag="VFL49_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7910697.1"
                     /translation="SDHAGVMSAAVPGFVIPTDRNGQLWIHFAPHDKARYVSAVDVLE
                     GRVPADRVAQRLVLIGTSAVGLLDSKTTPVDPLMPGVEVHAQVLENVLTNSVLSAPNY
                     AIGVELVAALLLGLVIIWLAPTLNPLLLLLVGATVVAISMGASWYFYVQNRLLFDFTY
                     PLMSSVLVYLTLVFSNYISEQAQRRRIRSAFGQYLSPALVEQLALSPDKLVLGGEDRE
                     MSILFSDVRGFTTISELYKDDPQGLTSLMNSFLTPLTNAIIEHKGTIDKYMGDAIMAF
                     WNAPLNDADHEINACDAALEMLRRVDTLNSQREQQAKENGQRFIPLKVGVGINTGRCV
                     VGNMGSDLRFNYSVLGDPVNLASRLEGQSKNYGLPIILGSRTASAVGDKFAVLELDCI
                     TVKGKTEPESVYTILGRSDIAGSDRFGQLRQNVNDMLTRFRRQDFSGATQAIERCRKF
                     GDGFGLGYLFDLYSDRIRVFEQSPPPADWNGVFVLETK"
BASE COUNT          642 a         1037 c         1098 g          682 t
ORIGIN      
        1 ttcgcgcctt caacgatgaa agcctcggcg atcgcgcggc cgatgccctg accggccgcg
       61 gtgaccaacg ccagctttcc ttgcagtcgg ttgctcacgg aacgatatcc tcaatggttg
      121 tcgcgcggga cgccgttctg ggcgacgcgc tgatacttca ccgcgggctt cagcaccatg
      181 ccatccgcca gctggtccac catggagcgc tggatttcct gccatggcgt ctggcttgcc
      241 gggtagcgaa agccgccgcg cttctgcagt gccgcgcgcc gccgcgccag ctcggctttt
      301 gagatcagga tgtcggcggt ccccttgcgc aggtcgatgc gaacgcggtc gccggtcttg
      361 agcaaagcga ggccgccgtt cgcggccgcc tccggcgatg cattgaggat ggatggcgag
      421 ccggacgtgc ctgactgccg tccatcgccg atgcagggca gggaggtgat gccctttttg
      481 atcagcgctg ccggcggctg catgttgacg acttcggccc cgcccggata gccgatcgga
      541 ccgaccccgc gaatgaacaa catcgtgtac tcgtcgattt tcaaagatgg atcgtcgatg
      601 cggtggtggt agtcctccgg tccttcgaag accactgcgc ggccttcgaa cgcgtctttg
      661 tctttggggt tggacagata gcgcttgcga aactcgtcag agatgacgct ggtcttcatg
      721 attgcagaat cgaacaggtt accgttgagc accttgaagc cggcgtgttt tttcattggt
      781 gaacggtaag gcttgatgac gtcggcattg gtgctctgcg cccgcttcac gttttcgccc
      841 atcgtcttgc cgttaatcgt caagacatcg gcgtggatgc gtttggcctt gagcaattcg
      901 tgcatgactg cgggcagccc gccggcacgg taatattcct cgccgagata ttcgccggcg
      961 ggctgcatgt tgaccagaag cggcacatcg aagccgatct tctcccaatc ctcgatcgaa
     1021 agcttcacgc cgacatggcg tgcaatggcg ttgatgtgaa cgggcgcgtt ggtcgatccg
     1081 ccgatggccg aattggcaac gatcgcgttc tcgaacgcct tgcgtgtgag aatatccgac
     1141 ggcttcaggt cctgttcgac gatctcgaca gcgcgcaggc cggtttcata ggcaatttgg
     1201 gcgcgctccc tatagggcgc ggggatggcg gcgcagccgg gcagggacat gccgagtgcc
     1261 tcggcgagcg cattcatggt cgaggccgtg cccatggtgt tgcaatggcc gatcgacggc
     1321 gccgtggacg cggcgatctg gatgaagtcc tcggcgtcga tgcggccggc cgccatctcc
     1381 tcgcgcgctt tccagattgc agtgccggag ccggcgcgct cgcctttcca ccagccgttg
     1441 agcatcggcc cgccggacag aacgatggcc ggcgtgttta cggtcgccgc ggccatcagg
     1501 caggcgggcg ttgtcttgtc gcagccggtc atcagcacga caccgtcgag cggatagccg
     1561 tagaggacct cgacaaggcc gagataagcg agattgcggt cgagcgccgc tgtcgggcgt
     1621 ttgcccgttt cctggatcgg gtgacaggga aactcgaagg cgatgccgcc ggccacgcga
     1681 attccgtcac gcacgcgctt ggcaagctcg agatggtggc ggttgcaggg agacaagtcg
     1741 gagcccgtct gcgcaatgcc gatgaacggc ttgcccgacc gcaactccgc cgacgtcagg
     1801 ccgtaattga gaaaacgctc gaggtagagc gccgtcatgc ccggattatc ggcattgtcg
     1861 aaccagagct gcgatctcag gcgtcgcgcg gattttcggg aagtctttgc catttgctgc
     1921 caaccatgct gccacgtcgg ccatagatga tcgcaaagac gcagggactg caagaagcgc
     1981 gcggcagacg ctatttcgtc tccagcacga agacgccgtt ccagtccgcc ggcggcgggc
     2041 tttgctcgaa cacccggatg cggtcggaat acagatcgaa caggtagccc aggccgaagc
     2101 cgtctccgaa cttgcggcag cgttcaattg cctgcgttgc accggaaaaa tcttgccggc
     2161 ggaagcgcgt gagcatgtca ttgacgttct ggcgcagctg gccaaatcga tcgctgccgg
     2221 caatatccga gcggccgaga atggtataga ccgactccgg ctcggtcttg cctttcacgg
     2281 tgatgcaatc gagctccagc accgcgaact tgtcaccaac cgccgacgcc gtgcgcgatc
     2341 caaggatgat cggcagacca taattcttcg actgcccttc cagtcgcgat gccaggttca
     2401 ccggatcgcc gaggacggaa tagttgaacc gcaaatccga tcccatgttt ccgacaacac
     2461 atcggccggt gttgatgccg acgccgacct tgagtggaat gaagcgctgg ccgttttctt
     2521 ttgcctgctg ctcgcgctgg ctgttgagcg tgtcgacacg tcgcagcatt tcgagcgccg
     2581 catcgcaggc attgatctca tggtcggcgt cgttcagcgg cgcattccag aacgccatga
     2641 tggcgtcacc catatactta tcgatagtgc ctttgtgctc gatgatggcg ttggtcagtg
     2701 gcgtgaggaa gctgttcatc agcgacgtca ggccctgcgg atcgtccttg tagagttcgg
     2761 atatagtggt gaaaccgcgc acgtcgctga aaagaatgct catttcgcgg tcttccccgc
     2821 cgagcacgag cttgtccggc gatagcgcga gctgctcaac gagcgcgggc gaaaggtact
     2881 ggccgaaggc cgagcggatg cggcggcgct gcgcctgctc gctgatgtag ttgctgaaca
     2941 ccagcgtgag atagacgagc acgctcgaca tcagcggata ggtgaagtcg aacagcagcc
     3001 tgttctgcac gtagaagtac cacgaggcgc ccatcgatat cgcaacgacc gttgcaccga
     3061 ccagaagcag caggagcggg ttcaatgttg gcgccagcca tatgatgaca aggccgagaa
     3121 gaagcgccgc gaccagttcg acgccgatag cgtagttcgg cgcggacagg acagaattcg
     3181 tcagaacgtt ttcgagaacc tgcgcgtgca cttcgacgcc cggcatcaag ggatcgaccg
     3241 gcgttgtttt tgagtcgagc aggccgacgg cggacgttcc gatcagcacc aggcgctgcg
     3301 ccacgcgatc ggcgggcaca cgtccttcaa gcacgtcgac ggccgacaca tagcgcgcct
     3361 tgtcatgggg cgcgaaatgg atccacagct gtccgtttcg gtcggtcggt atcacgaagc
     3421 ctggcaccgc cgcgctcatc acgccggcgt ggtccgagc
//
LOCUS       DASSRL010000076         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1069392, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000076 DASSRL010000000
VERSION     DASSRL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069392"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFL49_01585"
     CDS             complement(<1..725)
                     /locus_tag="VFL49_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7910698.1"
                     /translation="MKYSTDFPSIKDQVSPQEWQARLDLAACYRLVDVYGMTDLIYNH
                     ITARVPGEDDVLLINLYGLLYKEITASSLVKIHVDGNIIWKPDTDYDINKSGYVIHGA
                     IHKARPDVVCVLHTHTRAGMAVAAMKCGLLPLSQTSIRFVGHVGYHDYEGPAVELDER
                     ERLVRDLGPHEALIMRNHGLLTCGATVQQAFNTMYQLELSCRSQVDAMAARTELTMPG
                     ENVLAHTAHLYQPGTRRPYGILE"
     gene            complement(1095..1709)
                     /locus_tag="VFL49_01590"
     CDS             complement(1095..1709)
                     /locus_tag="VFL49_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7910699.1"
                     /translation="MSGKVRSLRLEPLPPETTPEMKEEFDSFFRTLGFVPNSVLTMQR
                     KPKLAKAFVQIQRAIWDPDSKVDRGFKRLVAHAASRTANDPYSMAHTASGALHFGIDA
                     AKLAAVRDYRTSPLFNDAERVALDLAVSASSTPNAVTEQTFSGLRRHWSEEQIVELVG
                     VIAVAGFLSRWNVTMATPIEPEPNQVGEKYLAPLGWDAGQHKRG"
     gene            complement(1706..2113)
                     /locus_tag="VFL49_01595"
     CDS             complement(1706..2113)
                     /locus_tag="VFL49_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910700.1"
                     /translation="MHGLYPNDASIYDSIGRLLIVAFFVIVWVRNLSSHHIEDHVKRL
                     TFFKAPLPRVTFWAGMALDAVGCALLLINWHPAIGALCLIVFTILASLLLLRFWEMDD
                     PMKRTGMQNGFLANIAVTGGLFLLLQNVMQASA"
     gene            complement(2139..>2580)
                     /locus_tag="VFL49_01600"
     CDS             complement(2139..>2580)
                     /locus_tag="VFL49_01600"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7910701.1"
                     /translation="MGAYVFIPFMTLSQLPEILVNFLLGFDFSTTMVLIIIITMYLLL
                     GTFMEDFSMLILTLPVVMPVLKTLGVDLIWFGVIAVLIIQMGILSPPVGLAVFIVKGI
                     VPDVPMYTIFRGIWPFWYAMGALIILLLIFPQLALFLPNTMFGN"
BASE COUNT          540 a          740 c          782 g          518 t
ORIGIN      
        1 cattccagga tgccgtaagg ccgtcgcgtg cccggctggt acagatgcgc cgtgtgcgcc
       61 agaacatttt ctcccggcat tgtcagttca gtgcgcgcgg ccatcgcgtc gacctgcgag
      121 cggcacgaca gctcaagctg gtacatggtg ttgaaggcct gctgaacggt ggccccacag
      181 gtcagcaggc cgtgattgcg catgatcagt gcttcgtgcg gtccaagatc gcgaacaagc
      241 cgctcgcgct cgtcgagctc gacagctggg ccttcgtagt cgtgataccc gacgtgacca
      301 acaaaacgga tcgaggtttg cgacaacggc aacaacccgc atttcatggc ggcaaccgcc
      361 atcccggcgc gggtgtgggt gtgcaggaca cagacgacgt ccggccgcgc cttgtgaatg
      421 gcgccgtgga tcacgtagcc ggatttgttg atgtcgtagt ccgtatccgg cttccagatg
      481 atgttgccgt cgacgtggat cttcacgaga ctggaagcgg taatctcctt gtaaagcagg
      541 ccatagaggt tgatcagcag cacgtcgtcc tcaccgggaa cgcgcgccgt gatgtgattg
      601 tagatgagat cggtcattcc gtagacgtcg acgaggcggt aacaggccgc gagatcgagc
      661 cgcgcctgcc attcctgcgg agacacctgg tctttgatcg acggaaaatc ggtcgaatat
      721 ttcatgagtt ccgctttcga acggttgagc gataccgcgt ccaggcgcca agaataccgt
      781 caggcgcgga attggctagc cctcgttcaa actgcgcgga tactttaact gtcatgcaag
      841 gatttcttcg taccgacgat ttcgatgccg gtactttcgc gacacaggag atccacgttc
      901 caacgcggca tggtcaagtg ccgcaggtgc gccgcgccga ttccggttag caagctggtc
      961 gcgctggcag atgagatctc gttgcgttgt ccgaagtgcg gcgaccgcgg cttctatgca
     1021 aaacgagcga tcgtcgtgga agaattgcct gagcggcgcc gaaatccgcg gcactagctg
     1081 tagcgctgct gacgtcagcc gcgcttgtgt tgtcccgcat cccaacccag cggtgcaaga
     1141 tacttctctc ccacttggtt cggctcgggc tcgatggggg ttgccatcgt cacgttccat
     1201 cgcgaaagaa atccggcgac cgcaatgaca ccgacaagct cgacgatctg ttcttcgctc
     1261 caatgccttc gaagtccgga gaaagtctgt tctgtcacgg cgtttggtgt cgaactggcc
     1321 gataccgcaa gatccaacgc gacgcgttcg gcgtcgttaa acaacgggct cgttctgtag
     1381 tcgcggaccg cggccagctt ggcggcgtcg attccgaaat gcagcgcgcc gctggcggta
     1441 tgcgccatac tgtagggatc gttggccgta cggcttgcag catgcgcgac aagccgcttg
     1501 aacccgcggt cgaccttgct gtctggatcc cagattgcgc gctggatctg cacaaatgcc
     1561 ttcgccagtt ttggcttgcg ctgcattgtg aggacgctgt tcggcacaaa accgagtgtc
     1621 ctgaagaagg aatcgaattc ctccttcatt tccggcgtgg tttccggcgg cagcggttcg
     1681 agccgcagcg acctgacttt tccgctcatg cgctggcctg cattacgttc tgcagcagca
     1741 ggaagagacc gcccgtgacg gcgatattgg cgagaaagcc gttctgcatg cccgtgcgct
     1801 tcatcggatc gtccatttcc cagaagcgca gaagcagcag cgaggcgagg atggtaaaga
     1861 cgatcaggca gagcgcgccg atcgccggat gccaattgat cagcagcagc gcgcagccga
     1921 ccgcgtccag ggccatgccg gcccagaaag tcacgcgcgg caagggcgct ttgaagaaag
     1981 taagacgctt gacgtggtcc tcgatgtggt gacttgacag gttgcgcacc cagacgatca
     2041 cgaagaacgc aacgatcagc agtcgcccga tgctgtcgta gatcgaagcg tcgtttggat
     2101 aaagcccatg catggttgtc tcccctgtgt ttcggctttc agttgccgaa catcgtgttc
     2161 ggcagaaaca gtgcaagctg cgggaaaatc agcagcagga tgatcagcgc gcccattgcg
     2221 taccaaaacg gccagatgcc gcggaagatg gtgtacatcg gcacgtccgg cacgatgcct
     2281 ttgacgatga agacggcaag ccctaccggc ggactcagga tccccatctg gatgatcagc
     2341 actgcgatca ccccgaacca gatcaggtcg acgccgagtg tcttgagcac gggcatcacc
     2401 accggcagcg tcaggatcag catggagaaa tcctccatga acgttccgag caacagatac
     2461 atcgtgatga tgatgatcag aaccatcgtc gtgctgaagt cgaagcccag gaggaagttg
     2521 acgaggattt ccggcagctg cgacagcgtc atgaacggga tgaatacgta ggcgcccatc
//
LOCUS       DASSRL010000077         3666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_824458, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000077 DASSRL010000000
VERSION     DASSRL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824458"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VFL49_01605"
     CDS             complement(<1..1196)
                     /locus_tag="VFL49_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910702.1"
                     /translation="MAKKHKKQRHGATREQQAGMATAQGPRPANKRPTVLFGVGDADF
                     GYTESKILRLADRLKIHGEWQVKIAGHDQETLREAKQLGLDTQLVNIESPGITVDDRL
                     RATDEMIRETANIDIPGSTLPLWKVLAMDDFLSSLQLFGAQPSEVIDADLVVTPLMAV
                     DNNTRASCGLYTWMVAEAKRRGIPCVGLEVSPLGNKNTLSHLPVDHFAVKGEWSKQFL
                     LREGLARAEQVSVLRWEERYCLWPGQDEYTEAYMEREAQMREMLNVSWDEFIILVPHH
                     VAFLWETRKILEALAAIEAPFTVVIRVDSRTTRRQYPEREIVLQAYDKELRRLPRVLI
                     DERVGVGLLLQLADLVVSPFAGTTTERAALCRKPTIICQAMGQEGYQDESFCWEPRPE
                     KIPERI"
     gene            complement(1218..3236)
                     /locus_tag="VFL49_01610"
     CDS             complement(1218..3236)
                     /locus_tag="VFL49_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7910703.1"
                     /translation="MSSVRLPGKVLADIAGRPMLWHVINRLGRADTVHRIVVATSIAR
                     ADAAIEEFCRTHEIALYRGSETDVLDRYYRAAQWCDADVVVRVTGDCPLIDAEVVDRV
                     VGEFFAARCDYASNTLRATYPDGLDVEVFSRAALEKAWREARLCAEREHVTPYLRSSE
                     RFRLWSVESGELNAGQLRWTVDEPCDLQFVRSLYERFNGRGYFGFREILKALKEEPSM
                     AELNGAIARNEGYYRSLANEPAVEPQTRALSRSYELKAKAERLIPSCSQTFSKGPSQF
                     IQGVAPVFLARGSGSRVWDVDGNEYIDYPMALGPVILGHNYPAVTEAVVRQLQDGGAF
                     SLPHPLEVEVAELLVETIPCAEMVRFGKNGSDVTSAAVRVARAYTGRDLIACCGYHGW
                     QDWYIGTTTRRRGVPRAVQELTLPFEYNNSESLARLFSQHPEQIAAVIMEPVGVQDPA
                     RGFLQQVQELARRHGALLVFDEVVTGFRVALGGAQQHYGVIPDLACFGKALANGFPLS
                     AIVGRRDVMELFDEIFFSFTFGGEAVSLAAAKATIGEMRERNVIAHLWEQGQRLKDGY
                     HVLARAYGLEKFTECQGLPPHTVAVFRDERGQESLLLKSFFMQECLKRGVLFSGVQNI
                     CFSHSPADIDATLRVYRSAMELLAAALAAGNLAEKLEGEPVQPVFRRA"
     gene            complement(3254..>3666)
                     /locus_tag="VFL49_01615"
     CDS             complement(3254..>3666)
                     /locus_tag="VFL49_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7910704.1"
                     /translation="LEIDTEDTAAMLLRFDNGALGEVHLDYVQRAYSRSCQIIGDEGT
                     IRWDYGAGQVRWYSARSQEWSVFANPDGWEANQMYVDEMAHFLRCVERAQAPESSVFE
                     AAEVLRIALAAKDSAREQRCIDLGGPTWTQSARL"
BASE COUNT          727 a         1188 c         1052 g          699 t
ORIGIN      
        1 tggatgcgct caggaatttt ttccggccgc ggctcccagc agaaactttc gtcctggtag
       61 ccttcctgcc ccatcgcctg gcaaatgatc gtcggcttgc ggcacagcgc ggcgcgctcg
      121 gtcgtggtgc cggcgaaggg agaaaccacc agatcggcca attgcagcag caaaccgacg
      181 ccgactcgtt cgtcgatcag cacgcgcggc aggcggcgca gttctttgtc ataggcttgc
      241 agcacaatct cccgctccgg gtactgccgg cgggtggtgc gcgaatcgac tcgaataacc
      301 accgtaaacg gcgcttcgat ggcggccagc gcttcgagaa tcttgcgcgt ctcccaaagg
      361 aacgcgacgt gatgcggcac caggataata aattcgtccc acgacacgtt gagcatctcg
      421 cgcatctgcg cttcccgctc catataagct tcggtgtatt cgtcctgtcc cggccagagg
      481 caataacgct cttcccagcg cagtactgag acctgctcgg cgcgcgccaa accttcgcgc
      541 aaaagaaatt gcttcgacca ctcgcctttc accgcgaaat gatccacggg cagatgactc
      601 aaagtatttt tgttacccaa cgggctgact tccaaaccga cgcagggaat gccgcgccgt
      661 ttcgcttcgg caaccatcca ggtgtaaaga ccgcaggacg cccgagtgtt gttgtccacg
      721 gccatcagcg gcgtcaccac cagatcggcg tcgatgactt cgctcggctg tgcgccgaac
      781 aactgcagag agcttagaaa atcgtccatg gccagcactt tccagagcgg caaggtgctg
      841 cccggaatat cgatgttggc ggtctcgcga atcatctcgt cggtggcgcg cagccggtca
      901 tcgacggtga tgccgggaga ttcgatattg acgagctgag tatcgagtcc aagctgcttg
      961 gcttcgcgca gcgtctcttg atcgtggccg gcaatcttca cctgccactc gccgtgaatc
     1021 ttcaagcggt cggcgagacg caaaatcttg ctttcggtgt agccaaaatc ggcgtcgccg
     1081 acgccgaata gcaccgtcgg gcgcttgttc gccggacgcg gtccctgcgc cgtcgccatg
     1141 cccgcttgtt gctcacgcgt tgcgccgtgg cgctgctttt tatgtttttt ggccatgatt
     1201 tgttcgtctc tcctgcttta ggcgcggcga aacaccggtt gcaccggctc gccttcaagc
     1261 ttctccgcaa gatttcccgc cgccagcgcg gcagcgagaa gttccatcgc gctgcgataa
     1321 accctgaggg tggcgtcgat atccgccggg ctgtggctga agcaaatatt ttgcacgccg
     1381 gaaaagagca cgccgcgttt taaacattcc tgcatgaaaa aactcttcag cagcagagat
     1441 tcctgcccac gctcatcgcg gaaaaccgcg acggtgtgcg gcggcaaacc ctggcattcg
     1501 gtgaattttt ccaaaccgta cgcgcgcgcc agcacgtgat agccgtcttt gagccgctgc
     1561 ccctgctccc ataggtgcgc gatgacgttt cgctcgcgca tttcaccgat ggtcgccttg
     1621 gcagcggcca gcgacacggc ttcgccgccg aaggtgaaag aaaagaaaat ttcatcgaaa
     1681 agctccatca catcgcgccg gccgactatc gccgagagcg gaaaaccgtt ggcgagcgct
     1741 ttaccgaagc aagccagatc aggaatgacg ccgtagtgct gctgcgcgcc gccgagggcg
     1801 acgcgaaacc cggtgaccac ttcgtcgaac accagcagcg cgccgtggcg ccgcgcgagc
     1861 tcttggactt gctgcaggaa accgcgcgcg ggatcttgca cgccaacggg ctccatgatc
     1921 accgcggcga tttgctcggg atgttgactg aacaaacggg cgagactttc gctgttattg
     1981 tattcgaacg gcaaagtcag ctcctgcacc gcccggggca cgccgcgccg ccgtgtcgtc
     2041 gtgccgatgt accagtcctg ccaaccgtgg taaccgcagc acgcgatcaa gtcgcgtccg
     2101 gtgtaggcgc gcgcaacccg caccgcggcc gaagtcacat ccgaaccgtt cttgccgaag
     2161 cgcaccattt ccgcgcaggg aatggtctcg accagcaatt cggcgacttc cacttccaac
     2221 ggatggggca aggaaaacgc gccgccatct tgcagctggc gcaccaccgc ctcagtcacc
     2281 gccggatagt tgtgaccgag gatgaccggt cccaacgcca tcggatagtc gatgtattcg
     2341 ttgccgtcga cgtcccaaac acggctgccg ctgccgcgcg cgagaaacac cggcgccacg
     2401 ccctgaataa actgcgacgg acccttgctg aaagtttgcg agcacgacgg gatcaagcgc
     2461 tcggccttgg ccttgagctc gtaggaacgg ctgagcgccc gcgtttgagg ttctaccgcg
     2521 ggttcgttgg ccaaagatcg ataatatcct tcattgcgcg cgatggcgcc gttgagctca
     2581 gccatggatg gttcctcttt caatgctttg agaatctcgc gaaaaccgaa atacccccgg
     2641 ccgttgaaac gctcgtagag cgagcggacg aattgcaagt cgcagggctc gtccacggtc
     2701 caacgcaact gcccggcatt caactctcca gactcgacgc tccataggcg aaagcgctcg
     2761 gagcttctca aatacggcgt cacgtgctcg cgttctgcgc agaggcgcgc ctcgcgccat
     2821 gccttttcca acgccgctcg ggaaaatact tcgacgtcca aaccatccgg gtaagtggcg
     2881 cgcagggtat tggatgcgta atcgcagcgc gccgcgaaga actcgccgac cacgcgatcg
     2941 accacctcgg catcgatcaa cgggcagtcg ccggtcacgc gcacaaccac gtcggcgtcg
     3001 caccattggg cggcgcggta atagcgatcc aatacatcgg tctcgctgcc gcggtagagc
     3061 gcgatctcat gcgtacggca aaattcctcg atggcggcgt ctgctcgggc gatggaagtc
     3121 gcgacgacaa tgcgatgcac agtatccgcc cggcccagcc ggttgatcac gtgccacagc
     3181 atgggacggc cggcgatgtc cgccagcacc ttgccgggca aacgcaccga gctcatgcgc
     3241 gcctgtacga tcgctacaac ctggcgcttt gcgtccaagt cggacctccc aggtcgatgc
     3301 agcgctgctc gcgcgccgag tccttcgccg ccagcgcgat gcgcaaaacc tccgcggctt
     3361 caaagacgct cgactccggc gcctgtgccc gctcgacaca acggagaaag tgcgccatct
     3421 catccacgta catttgattg gcttcccaac cgtcagggtt cgcaaagacg ctccattcct
     3481 gcgagcgcgc cgaataccag cgcacctgcc ccgcgccgta atcccagcga atcgttcctt
     3541 cgtcgccgat gatttggcag cttcggctgt aggcgcgctg cacgtagtcg agatgcacct
     3601 cgcccaacgc gccgttgtcg aagcgcaaaa gcatggcggc ggtatcttcg gtgtcgattt
     3661 caagcc
//
LOCUS       DASSRL010000078         5251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1716678, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000078 DASSRL010000000
VERSION     DASSRL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716678"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFL49_01620"
     CDS             <1..651
                     /locus_tag="VFL49_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7910705.1"
                     /translation="RHPTWGVVAPADYSPGASDPFLHELSQFVVARVLADSTQFAAGN
                     HEVQISVRLPLQALEDMQFIDRMLAHLPDKVRQNGFLIAVDCVDLINELGLVRRIAIQ
                     LAARNIGLSIDGIDAQGALLAGRRELPVVEMKVGRTYIRGCGDDRIKQAHCAQIIAIA
                     RDTGAKSVAEGVETQSDFLVVRDLGFDLLQGHMLAKPMAPHKFERAMLARRYAAVA"
     gene            complement(662..1546)
                     /locus_tag="VFL49_01625"
     CDS             complement(662..1546)
                     /locus_tag="VFL49_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HET7910706.1"
                     /translation="MAYSNVLVGAGTRPAQPVIRRIGIADLQEALAKGIDDFYAMPTH
                     AMFLCVIYPVVGFLFARLAFGYSILPLLYPLASGFALVGPVAALGLYELSRRREAGLP
                     TSATHAFDVLQSSSIGAITALALLLLTVFVIWVAVANAIYVATFGYATPTSLEIFAYD
                     VLTTRAGWTLILVGNLVGLLFAILVLTISVVSFPLLLDRDVGAAVALLTSVRAVARNP
                     FTMMLWGLIVAALLVVGSLPLFFGLTVIMPILGHATWHLYRRVVEPQAAPPPTLPEEQ
                     RERRYAADFPAVLFPWAR"
     gene            complement(1710..2819)
                     /locus_tag="VFL49_01630"
     CDS             complement(1710..2819)
                     /locus_tag="VFL49_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HET7910707.1"
                     /translation="MEEPFGFLESELDRIVATTMGPAPANNVVQISDTLRSQTTLRER
                     ERRFRELLDALPAAVYTTDAAGRITYYNDAAVELWGHRPPLGNSEWCGSWKLYWPDDT
                     PMAHDECPMAIALKEDRVVRGMEAAAERPDGTRVPFIPYPTPIHDEAGRLIGAVNMLV
                     DISDRKRAEEQQALLVRELHHRVKNTLATVQAIMGSTARTVDNIEDFKTAIFGRIQSL
                     AKTHLLLADDAKAVNFADVLRSELDAFDDGSGGRVTLEGPDVALPSQIAVSLGMAVHE
                     LTTNAAKHGALSVYGGKVTVTWSITIDATRRTLVFDWIEDGGPPVTEPAREGFGSRLL
                     DFVLPGQIQARTRIEYAQQGVRVHCSVPLPAETKN"
     gene            2999..3832
                     /locus_tag="VFL49_01635"
     CDS             2999..3832
                     /locus_tag="VFL49_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7910708.1"
                     /translation="MATLKSLIILAILAYAGALALMYLFQRSLMYFPDPVRTSPAAAG
                     LPRAEELILHTADGETLIAWHVPPQGTKPVVIYFHGNAGALNLRAGRFGWLVGDGFGL
                     VALSYRGYGGSSGKPSEAGMLLDASAAYDFAAARYPPERIVLWGESLGTGVAVALAAE
                     RRIGGLILDAPFTSAADIGAAAYPFVHVRWFIKDTFRSDLRIGRAKAPILVLHGEQDR
                     IVPIAFGEKLFALANEPKQMVRFPSGGHVDLDDHGAAKVVKTFLNNLPMCDEGPQCSR
                     R"
     gene            complement(3863..4120)
                     /locus_tag="VFL49_01640"
     CDS             complement(3863..4120)
                     /locus_tag="VFL49_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910709.1"
                     /translation="MVDNGASADCNGDFDVMVARANIARAIAFGNSPARLFPAEFDVG
                     LVLLARRTAGIAHFRNIKTGALFGGTLFRVGNCAPARMPAA"
     gene            complement(4095..4388)
                     /gene="rpsU"
                     /locus_tag="VFL49_01645"
     CDS             complement(4095..4388)
                     /gene="rpsU"
                     /locus_tag="VFL49_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7910710.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRGHYEKPSEKKA
                     REKAEAIRRARKLARKKMQREGLLPMKPRPTPGAGPGGSGGGHGRSGGQRRFG"
     gene            complement(4534..5184)
                     /locus_tag="VFL49_01650"
     CDS             complement(4534..5184)
                     /locus_tag="VFL49_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7910711.1"
                     /translation="MALTLYFHPLSSFCQKALIALYENGTAFTAQKVDLMDEKENAAF
                     KQMWPVGKFPVLRDEKNGNTIPESTTIIEYLAQHYPGPVKLVPKEADRAFIVRAQDRF
                     YDLNVHLLTQKVITDRLRPAGQNDTFGVEHARALLQTALGIIDKEMAKKTWAIGDAFT
                     MADCAAAPTLFYYNRAVTPLAGSFDNVAAYLDRLVRRPSYARALKEAEPFLKYVPI"
BASE COUNT          993 a         1581 c         1636 g         1041 t
ORIGIN      
        1 cgccacccta cctggggcgt cgtggcgccg gctgactaca gtcccggcgc gagcgatcct
       61 tttctgcatg agctgtcgca gtttgtggtc gcccgggtgc tggccgactc gacgcagttt
      121 gctgcgggta atcacgaggt ccagatttcc gtccgcttgc cattgcaggc gttggaagac
      181 atgcagttca tcgaccgtat gctggcgcac cttcccgaca aggtccgcca aaacggtttt
      241 ttgattgccg tcgattgcgt cgatttgatc aacgagctcg gcttggttcg gcgtatcgcc
      301 attcagttgg cggcgcgcaa catcggactc tcgattgacg gcatcgatgc tcagggcgcc
      361 ttacttgccg gtcgtcgcga attgccggtg gtggaaatga aagtcggccg tacatacatc
      421 cgcggctgcg gcgacgacag gatcaagcaa gcgcactgcg cgcagatcat cgctatcgcg
      481 cgcgataccg gcgcgaaatc cgtcgccgaa ggcgtggaaa cgcaatcgga ctttctcgtc
      541 gttcgcgatc tcggcttcga tcttctgcaa ggccatatgc ttgcgaagcc gatggccccg
      601 cacaaattcg agcgagcgat gctcgcgcgc cgatatgcgg ccgtggccta acggccgggt
      661 gctatcgcgc ccaaggaaaa agcacggccg ggaagtccgc cgcatagcgg cgctcgcgct
      721 gctcctccgg aagcgtcggt ggcggtgccg cctgcggctc gaccacgcgg cgatagagat
      781 gccacgtggc gtgcccgagg atgggcatga tgaccgtcag cccgaaaaac agcggcagcg
      841 atccgaccac aagcagcgcg gcgacaatga ggccccacag catcattgtg aaagggttgc
      901 gggcaactgc gcgaaccgaa gtcagcagcg ccacggccgc gccgacatcc cggtcgagca
      961 gcagcgggaa tgatacgacg ctgatggtca acaccaggat ggcgaagaga aggcccacca
     1021 ggttaccgac gaggatgagc gtccatccgg cgcgggtggt gagcacgtca taggcgaata
     1081 tttccagaga ggtcggtgtg gcataaccga atgtcgcgac gtaaatcgca ttggcgaccg
     1141 ccacccaaat gacgaatacg gtcaacagga gcaatgccag tgccgtgatc gcgccgatcg
     1201 aggacgattg aagcacgtcg aacgcatgcg ttgcggatgt cggcaggccg gcttccctgc
     1261 gccggctcag ctcgtagaga ccgagcgcag caaccggtcc caccaaggca aaaccgctcg
     1321 ccagcggata aagcagcggc aggatcgaat agccaaatgc cagccgggca aacaaaaagc
     1381 ccacgacggg atagatcacg cacaggaaca tggcgtgcgt cggcatcgcg tagaaatcgt
     1441 cgatgccctt ggccagggcc tcctgcaggt cggcaatacc gatgcggcgg atgaccggtt
     1501 gtgccggacg tgtgccggca ccgaccagaa catttgagta ggccatgcga tacctcccga
     1561 gacttgcggc ccggacggtg tcgcgcctga gacggacata aatctttttc cggctcttta
     1621 acgcgtcacg tcatgagcca tacgcaaaca ttatagtgcc ggttcagcgc ggcatcgatc
     1681 acaagtccgc gcgtctgcac gaatcaatgt tagtttttcg tttccgccgg aagcggcacg
     1741 gaacaatgca cgcgaacgcc ttgctgtgca tactcgatcc gcgtgcgggc ctggatctgg
     1801 ccgggcagga cgaaatcaag aaggcgtgag ccaaatcctt cgcgggccgg ttccgtcacc
     1861 ggcgggccgc cgtcttcgat ccagtcgaac accagcgtgc gccgcgtggc atcaatcgtg
     1921 atgctccagg tcactgtcac cttgccgcca tagacggaca gggcgccgtg tttcgccgca
     1981 ttcgtcgtga gttcatggac cgccatgccg agcgacaccg cgatttgcga tgggagcgcg
     2041 acgtccggac cctccagtgt gacgcggccg ccgctcccgt cgtcaaaggc atcgagttcg
     2101 cttctgagaa cgtcggcgaa attgaccgcc ttcgcatcat ccgcaagaag gaggtgtgtt
     2161 ttcgcgagag actggatgcg tccgaatatt gcggtcttga agtcctctat gttgtcaacg
     2221 gtgcgcgcgg tcgagcccat aatggcctgc acggtggcga gcgtgttctt tacccgatgg
     2281 tgcaattcgc gcaccagcaa ggcctgctgc tcctcggcgc gcttgcggtc gctgatgtcg
     2341 acgagcatgt tgacggcgcc gatcagccgg ccggcttcgt catgaattgg cgtcgggtag
     2401 gggatgaagg ggacccgcgt gccgtcaggc cgctcggcgg ctgcttccat tccgcgcaca
     2461 acgcggtctt ccttcagggc gatggccatt gggcattcgt cgtgtgccat cggcgtgtcg
     2521 tcgggccagt aaagcttcca ggatccgcac cactcgctgt tgccgagagg cgggcgatga
     2581 ccccaaagtt ctacggcggc atcattgtag taggtaatgc gtccggcagc gtcggtcgta
     2641 taaacggccg ccggcagcgc atcgagcagt tcgcggaagc ggcgctcgcg ttcacgaagt
     2701 gtggtctgcg atcggagggt gtcggaaatc tggacgacgt tattggcggg cgcaggaccc
     2761 atcgtggtgg ccacgatccg atccagctcg ctctcaagaa atccaaaagg ctcttccatg
     2821 gcccccgatc aacccgcaaa atttcggtct ccgctcttga cggcgaagca cctttcgata
     2881 cccagcggta acgcgcctgc gccgcaatgg ttgctcgcat ccacgcaaat ttcagccata
     2941 gcgaccggta tcattaacaa acagcagcca gaccgggcgg ccgggtgaga gggcgcgaat
     3001 ggctaccctg aaatcgctga taattctggc cattctggcc tacgctgggg ccctggcgct
     3061 gatgtatctg ttccagcgtt cgctgatgta cttccccgac cccgtccgca catctccggc
     3121 cgctgccgga ttgcctcggg ccgaagaact gatcctgcac actgccgatg gcgagactct
     3181 gattgcatgg cacgtgccgc cacaggggac aaaaccggtc gtgatctatt tccacggcaa
     3241 tgccggcgct ctcaatctcc gcgccggtcg gttcggatgg ctggtcggcg atggtttcgg
     3301 tctggtggct ttgtcgtatc gcggctacgg cggctcgtcc ggcaaaccat cggaggctgg
     3361 gatgctgctg gatgcatcag ccgcttacga cttcgcggca gcgcgttatc cgcccgaaag
     3421 gatcgttctt tggggtgagt cgctcggcac cggtgttgcg gtagcgcttg ctgccgagcg
     3481 tcggatcggc gggcttattc tcgatgcgcc gttcacatcg gctgccgata tcggtgccgc
     3541 cgcttatccg ttcgtgcacg tccgttggtt catcaaagat actttccgtt ccgatctgcg
     3601 tatcggtcgc gcgaaggcgc cgatactcgt tctgcacggc gagcaagacc ggattgttcc
     3661 catcgcgttc ggcgaaaagc tgtttgcgct cgccaacgag ccgaagcaga tggtgcgctt
     3721 cccgtccggc gggcacgtcg acctcgacga tcacggcgcc gccaaagtgg tcaagacatt
     3781 tctgaacaac ttgccgatgt gcgacgaggg accacaatgt tcgcgacgct gaaacatgcc
     3841 gccggtgcgg tcgcgccgat ctttacgctg ccggcattcg cgccggcgcg cagttgccaa
     3901 cgcgaaagag cgtgccgccg aagagtgcgc ctgttttgat gttccgaaag tgcgcgatgc
     3961 ctgctgtgcg acgcgcaagc agaacgaggc cgacgtcaaa ttctgctgga aatagacgag
     4021 ccggcgagtt cccaaaagca atagcgcggg cgatattggc ccgcgccacc atcacatcga
     4081 aatcgccgtt acaatcagcc gaagcgccgt tgtccaccac tgcggccgtg tccaccgccg
     4141 ctgccgccag gaccggcacc cggggtcgga cggggcttca tcggcaggag gccttcgcgc
     4201 tgcatcttct tgcgcgcgag cttgcgggcg cggcggatcg cttccgcctt ttcacgtgcc
     4261 ttcttttcgg atggtttttc gtagtggccg cggagtttca tctcgcggaa aatgccctcg
     4321 cgctgcatct tcttcttgag cgccttcagg gcctggtcga cgttgttgtc gcgaacgaga
     4381 acttgcacgc ttgctccttg gttgttgctc ggacgggtta aggcaaaggc cccgaaaaac
     4441 gggggctttg agtcgggcgg ctctagcaga aaaggcgtta agagtccatt cgacgcggtt
     4501 ttcatattgg gacccaaggc ttaacgggca agctcatatc ggcacatatt tgaggaaggg
     4561 ctcggcctcc ttgagcgcgc gcgcatagga cggccgcctc accaggcggt cgagataggc
     4621 ggccacgttg tcgaaggagc cggccaaagg cgtcaccgcc ctgttgtaat aaaacagcgt
     4681 cggcgcggcg gcacagtcgg ccatcgtaaa tgcgtcgccg atagcccagg tcttttttgc
     4741 catctccttg tcgatgatgc cgagggccgt ctgaagcagg gctcgtgcgt gctcgactcc
     4801 gaatgtgtcg ttttgtccgg cgggccgcag ccggtccgtg atgacctttt gcgtcagcag
     4861 gtgaacattg agatcgtaaa accggtcctg cgcgcgcacg atgaacgccc gatcggcctc
     4921 tttcggaaca agcttgaccg gccccgggta gtgctgcgcc aggtattcga tgatcgtcgt
     4981 ggattccgga atcgtgttcc cgttcttttc gtcgcgcagc acgggaaact tcccgaccgg
     5041 ccacatttgc ttgaaggccg cattctcttt ctcatccatc aggtccactt tttgcgcggt
     5101 gaacgccgta ccgttttcgt aaagcgcgat cagtgccttc tggcagaacg acgaaagcgg
     5161 gtggaaatag agcgtcagcg ccatcatgaa cctccgggct gcgggtcaag gtctcactgt
     5221 tggaaatggc agtcctgagg caaatgccaa g
//
LOCUS       DASSRL010000079         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1156907, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000079 DASSRL010000000
VERSION     DASSRL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156907"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..776)
                     /locus_tag="VFL49_01655"
     CDS             complement(258..776)
                     /locus_tag="VFL49_01655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7910712.1"
                     /translation="MKKSDTSKKGTALEIASFTTGLVSADEIASRSGLQFLTDMANGI
                     LPQPPMCATLGFRLIEVADGYARFDGEPEFRHYNPIGTVHGGFAATLLDSALGCAIFT
                     TLAKGEAWTTLELKLNMVRPITKETGTVRAEGRIVHRGRTVGTADGTVKDRAGKLYAH
                     ASTTCMIFPAKN"
     gene            1111..1527
                     /locus_tag="VFL49_01660"
     CDS             1111..1527
                     /locus_tag="VFL49_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910713.1"
                     /translation="MTITLIDTTMPSRHPLVIAGFAVALMLTATPAAFAGNRVHAADV
                     AKVLSGKNFRIDCIDGTHGRGQVTDQGIANLSYRRPADGREANDSTLIQVKGVDMCLS
                     WQRFAGGGRGCYPVSEESAGRYRLSTGPLWCDISMK"
     gene            complement(1569..>2357)
                     /locus_tag="VFL49_01665"
     CDS             complement(1569..>2357)
                     /locus_tag="VFL49_01665"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET7910714.1"
                     /translation="DDRPCRTVESAAILFTVLTAFLEIRHFINGGDIYRQTSALGELA
                     LQVCTGLAMTIGLEHVRHRTGSIVHDIGALVIAALTFAGIVLGLWLFCNPLFTGEQVG
                     SAFFNLILLGYGIPAVLAIVLALRTRHVRPPPYRICAAVVAVLLMLSYLSLEVRTLYH
                     GPVLDRGVTTDAEQYTYSVVWLAYGVALLLFGIALRSQPARLASAAITLLTVAKVFLF
                     DMAGLTGVFRALSFIGLGLVLVGIGWLYQKLLFPPRSTTTTAPA"
BASE COUNT          482 a          688 c          731 g          456 t
ORIGIN      
        1 cgctgcgcga tgtcattgca ggaaatctcg aagtgtacgt tgccgagttg ctgatcggtg
       61 tcgccctgcg ccacgatcgg cgcggccgag gacagcaaga gtgtcgccgc acaggcggtc
      121 ccgactatcg cgaacctacg catagccaac ctcctatcga tgatgaaaac accggcattc
      181 gcgcccgagc caattttacg ccttttcatt ccggccggat agcgagagat gtggtgacat
      241 ttccattaca accgggctca attcttggcc ggaaaaatca tgcaagttgt actggcatgg
      301 gcatagagct tgccagccct gtccttgacc gtgccatctg cggtgccgac ggtgcgacca
      361 cgatggacga tacggccttc ggcgcgcacc gtgccggttt ccttggtgat cggtctcacc
      421 atgttgagct ttaactctag cgtggtccag gcctcgcctt tggccagcgt agtgaatatg
      481 gcgcaaccga gcgccgagtc gagcagcgtc gcggcgaatc cgccatgcac ggtcccgatc
      541 ggattgtagt gacgaaactc cggctcgcca tcgaagcgag cgtagccgtc ggccacctcg
      601 atcagacgaa agccaagcgt ggcgcacatc ggcggctgcg gcaggatgcc gttggccata
      661 tcggtgagga attgcagacc gctgcgcgat gcgatctcgt cggcagatac gaggccggtg
      721 gtgaatgacg cgatctccaa cgccgttcct tttttggagg tatcagattt cttcatgcgg
      781 ctggtccgtt gtgaggcctt cacgccttgc agtgtcactg ttaatgcttg tcggccgtgc
      841 ttgaacgatt gcgccgagac gacgctcgat ccgatcgcgc tttgcaaaat agttcattgg
      901 ttaaagcaac gaacgttcgc cgcttggtaa gcgagtcgct gcaatcgacg agtgttttga
      961 tgaatcgaag gtcatcgctc gccagcattc accattaact ccaggcagca tctgggttcg
     1021 cgcggcctct gcgattaacc gttgaaatag tttgggcgcg acagcggcgc gaagataaga
     1081 gatggatcgt catcgaaggg aggcctcttc atgacaatca cacttatcga cacgactatg
     1141 ccgagccgtc atccgcttgt catcgcgggc tttgcggttg cgctcatgct gactgcaacg
     1201 ccggcagcgt ttgccggcaa tcgcgtgcat gcggccgatg tggcaaaggt gctgagcggc
     1261 aagaacttcc gtatcgattg tattgatggg acccatggcc gcggccaggt taccgatcag
     1321 ggcatcgcca atctgtcata tcgcaggccg gctgacggcc gagaagccaa cgatagcacg
     1381 ttgatccaag ttaaaggcgt cgacatgtgc ctgtcgtggc agcgtttcgc cggcgggggc
     1441 cgcggctgct atccggtctc ggaagagtcc gctgggcgct atcggctgag cacaggtccg
     1501 ctgtggtgcg acatcagcat gaaataaacg cttcagcgca tctttgaacg gttggcggcg
     1561 ccccggaatc acgcgggcgc cgttgtcgtt gttgaccgtg gcggaaacag cagtttctgg
     1621 tagagccagc cgatcccgac cagaaccagt ccaaggccga tgaaggacaa tgcgcggaat
     1681 acgcccgtca atcccgccat gtcgaacagg aataccttgg cgacggttag cagcgtaatc
     1741 gcggctgaag cgagccgcgc cggctgtgag cgcagcgcaa ttccaaacag cagcagcgcc
     1801 acgccgtagg cgagccacac taccgagtag gtgtactgct cggcgtcggt ggtgacgccg
     1861 cgatcgagca ccggtccgtg gtagagcgtc cgcacctcca gtgagaggta gctcagcatc
     1921 agcagcaccg cgacgaccgc ggcgcagatg cgatagggcg gcggccgtac atggcgcgtc
     1981 cgcagcgcca gaacaatcgc cagcaccgcc ggaatgccgt agcccagcag gatgagattg
     2041 aagaaggcgc tgccgacctg ctcgccggtg aacagcggat tgcagaacag ccatagcccc
     2101 aacacgatgc cggcgaacgt cagcgcggcg ataaccagcg cgccgatgtc gtggacgatg
     2161 ctgccggtgc gatggcggac gtgctcgagg ccaatggtca tcgcgagccc ggtgcagact
     2221 tgcaatgcga gttcgcccag tgcgctggtc tggcgataaa tatcgccgcc gttaatgaaa
     2281 tggcggattt ccaaaaatgc agtcagcact gtgaacagga tcgccgcgct ctcgaccgtc
     2341 cggcacggcc tgtcgtc
//
LOCUS       DASSRL010000080         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1209440, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000080 DASSRL010000000
VERSION     DASSRL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209440"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(544..1233)
                     /locus_tag="VFL49_01670"
     CDS             complement(544..1233)
                     /locus_tag="VFL49_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7910715.1"
                     /translation="MTVKTAAGIMRQDRKTTMRALHRWIVSLAGVTLASAIAFQWLDR
                     PIALFFRHIVARPQTFAKLTYAPDPMVPLAVIVFVVLGLVSLSGRVLSRLQTCALLCS
                     LSLIVAELTKAQLKLVFGRTWPDTFRDSNPSFLHDGVYGFNFFHGGHAYASFPSGHTA
                     VTCAVISVLWIYYPRWRPLYALAVLAVAVGLIGANYHFLSDVIAGGFVGVSCGWMLTS
                     LWKAHEHFGKY"
     gene            1406..2077
                     /gene="npdG"
                     /locus_tag="VFL49_01675"
     CDS             1406..2077
                     /gene="npdG"
                     /locus_tag="VFL49_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET7910716.1"
                     /translation="MQNHPTIAILGGTGDLGSGLARLLAAGGYPVVIGSRDKDKAKQF
                     SAKLSSQLATEVRGDDNRGAAVSADIVVLCVPYSNHDHILQEIKPVMNGKILIDAVVP
                     LVPPKVSIVQLPATGSPAVVAQRVLGAGVQVAAAFHNVGAKKLHDGEKADCDVLVFAD
                     DAAVREQVIALASTVATRGVDGGVLANSVAAEALTSVLIAINRRYKIPGAGIHITGLP
                     VPEPR"
     gene            2081..2845
                     /gene="cofE"
                     /locus_tag="VFL49_01680"
     CDS             2081..2845
                     /gene="cofE"
                     /locus_tag="VFL49_01680"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET7910717.1"
                     /translation="MVAPSLTLSALAGLPLIKPGDDLGALLIDALRRARIVPQDNDVL
                     VVAQKVVSKAEGRIVDLKGVVPSARAIAVGKEVNKDARLVEIILSESSEIIRQKRDVL
                     IVAHRLGFIMANAGVDQSNVAGEDEQALLLPIDPDASAAALKARLDREFGVDIGVVVN
                     DSFGRPWRFGVVGVALGAAGIPVLQNMVGVPDLFGRKMRVTEVAVADEIAAAASLLMG
                     QGAEGLPAVHLRGLNWNAPAARVSALLRPKDQDLFR"
     gene            2842..>3065
                     /locus_tag="VFL49_01685"
     CDS             2842..>3065
                     /locus_tag="VFL49_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HET7910718.1"
                     /translation="MSAPVVANHVLALCGGIGGAKLALGLSRVFGAGLTVVVNTGDDF
                     EHLGLSISPDLDTVLYTLGGLADPERGWGRS"
BASE COUNT          634 a          870 c          966 g          595 t
ORIGIN      
        1 gatcgatgta gagcccaccc gtgccttgat ttagggtgct gaacactcgg accgaaaggc
       61 ctggcgctag gctatccagg ttcggcgaag ggtcaaaaga cgaaggcaga tttgcactta
      121 cgccgcctac aatcgaaaag cttgcagcct gcgcgtttcc gcaaacaaat gccgcaaaca
      181 aaagcgccgc gcccagcaag tgcgcgcgac gccgcgtatt ccaagcaaac atgatggaga
      241 agattccacg aaaaccgcca cacagtgcaa gtagagaagt acccttgagt ctgtcaaatg
      301 ctttctgtgg taaatgcctg gaacttaacg gaattattat cgaacgttcg tggacaaccc
      361 gtagactaac tgacggaaag tggcgggatt ttctggtccg gcgaataacg acccgcattg
      421 ttgcattggg tcaaagacgt ctgaatgacg caggccctaa cgactcttct cttctggatg
      481 aagttcagaa atttgaaaga gcttcttgaa ctcactcgag cgcgtgcgga agtgctcgcg
      541 ggcctagtat ttgccgaaat gctcatgcgc cttccacagc gaggtgagca tccagccaca
      601 ggacacgccg acaaagccgc cggcgatcac gtcgctgagg aagtggtagt tggcgccgat
      661 cagcccgacg gcgacggcga ggacggcgag cgcgtaaagc ggccgccagc gcggatagta
      721 gatccacagc accgagatca ccgcgcaggt cacggcggta tggccggacg gaaacgacgc
      781 gtaggcgtgg ccgccgtgga agaaattgaa gccgtaaacg ccgtcgtgca ggaaagacgg
      841 attgctgtcc ctgaacgtgt caggccaggt gcgtccgaac acgagcttga gctgcgcctt
      901 ggtcagttcg gcgacgatca ggctgaggct gcagagcaac gcgcaggtct gcagacgcga
      961 cagcacgcgg cccgacaggc ttaccagccc gagcacgacg aagacgatca cggccagagg
     1021 caccatcggg tcgggcgcgt aagtgagctt ggcgaaagtc tgcggccgcg cgacgatgtg
     1081 acggaaaaac agcgcgatcg gtcggtcgag ccattgaaag gcgatggccg aggcgagtgt
     1141 gacgccggcg aggctcacga tccagcggtg aagcgcgcgc atggtggtct tgcgatcctg
     1201 ccgcatgatg ccggcggcgg ttttgacggt catcttcacc tactacatta agacagtgtg
     1261 tggttccggt cgaatttcgc ggccggcgtg cttgcgccgc cgctggcttg tagcccgcac
     1321 gatcacattg attgctcaaa gatattgtct tcctataaat agtaccttaa cttcaaaaat
     1381 cgctcacttc gcatgcgata gcgatatgca aaaccatccc accatcgcca ttcttggcgg
     1441 caccggcgac ctcggatccg gactggcccg tctgctggcc gccggcggat atccggtggt
     1501 gatcggctcg cgcgataaag acaaggccaa acaattttcc gcaaagcttt cttcacaact
     1561 ggcaaccgag gtgcgcggcg acgacaaccg cggcgctgcg gtgtccgcgg atatcgtcgt
     1621 tctctgcgtg ccttattcca atcacgacca tatccttcag gagatcaagc cagtcatgaa
     1681 tggcaagatc ctcatcgatg cggttgtgcc actcgtgccg cccaaagttt cgatcgtcca
     1741 gctccctgcg accggctcgc cagccgtcgt agcgcagcgc gtcctgggag ccggcgtgca
     1801 ggttgcggcc gcgtttcaca atgtcggcgc caagaaactt catgacggcg aaaaggccga
     1861 ttgcgatgtg ctggtatttg ccgatgacgc ggcagtgcgc gagcaggtga ttgcgctcgc
     1921 cagcacggtt gcaacacgtg gtgtcgacgg aggtgtgctc gcaaattcgg ttgccgccga
     1981 ggcgctcacc tcggtcttga ttgcgatcaa tcggcgatac aagattcccg gcgccggaat
     2041 tcacattacg ggcctgcccg tgcccgagcc gcggtgaatg atggtcgcgc caagcctgac
     2101 gctgagcgct ctggcgggtc ttccattgat caagcccggc gacgatctcg gtgcgctgct
     2161 gatcgacgcc ttgcggcgcg cgcggatcgt gcctcaagat aatgacgttc tcgttgtcgc
     2221 acagaaagtg gtgtcgaaag cggaagggcg tatcgttgat ctcaagggcg tcgtgccgtc
     2281 cgcgcgagcg attgccgtcg gcaaggaggt caacaaggat gcgaggctgg tcgagattat
     2341 tctgtcggaa tcgagcgaga tcatccggca aaagcgcgat gtgctgatcg ttgcccacag
     2401 gcttggattc atcatggcga acgcaggcgt cgatcaatca aacgtggcgg gcgaagacga
     2461 gcaggcgctc ctgttgccga ttgacccgga tgcgtctgcc gccgcgctga aagcacggct
     2521 cgaccgtgag ttcggtgtcg acatcggcgt cgtggtcaat gacagtttcg ggcggccttg
     2581 gcggttcgga gtggttggcg ttgcccttgg tgcggccggg attcctgtcc tgcaaaatat
     2641 ggtcggtgtt cccgatctct tcggccgcaa gatgcgggtg accgaagtcg cagttgcgga
     2701 cgagatagcg gcagcggcct cactgctgat gggccaaggc gccgaaggcc tgcccgcagt
     2761 acacttgcgc ggactcaatt ggaatgctcc agccgcgcgg gtctcggcgc tgctgcggcc
     2821 caaggatcag gacctatttc gatgagtgcg ccggttgtcg caaaccatgt gcttgcactg
     2881 tgcggaggta taggcggggc gaaactcgcg cttggccttt cgcgcgtttt cggagcaggc
     2941 ctgacagtgg tcgtaaatac cggtgacgac ttcgagcacc tgggcctatc catttcgccc
     3001 gatctcgata ccgtgctcta cacactcggc ggcctggcgg atccagaacg cgggtggggg
     3061 cgttc
//
LOCUS       DASSRL010000081         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1664313, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000081 DASSRL010000000
VERSION     DASSRL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664313"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFL49_01690"
     CDS             <1..275
                     /locus_tag="VFL49_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7910719.1"
                     /translation="RKAATPAIQSLPTRSETPTRTLTDGSIAIRPKQAARLAGICRSN
                     LFRAIRDGHLPARKYGRCTLILVQDLWAWLEQLPGIAEGRNHQAGS"
     gene            348..1676
                     /locus_tag="VFL49_01695"
     CDS             348..1676
                     /locus_tag="VFL49_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HET7910720.1"
                     /translation="MEIFAPAPGWRRPSPLKTSIEGPGRVGLVIILVFAVVCGVWGVV
                     IPLAGGAMAPGALASDQGKKVIQHLEDGIIAELRVHDGDVVESGQPLVVLENVQTRAT
                     YDALQQQMWSLLAKHARLQAESEHRNIIEWPAELHASNAKIAAILNAQRLVFETRAAE
                     LATKKNILRQRIDQLNDQIRGLEAQLESAAGQGELITEELQGKEVLLKQGLMPKPEYL
                     RLKRNKVEIDGRHGEYLAQIAKAKQQIGETQMQILNADAERATQIATESDSVRIDLSN
                     TMEKLRSSEDVLKRTVVTAPIGGTVVNSNFKTIGGVVEKGRPILEIVPLGDALIIEAR
                     VTPHDVKAVHNGLNAQIHFTAYSSRSTPRIPGIVQSVSADRLFDDKTHQPYYLVKVNV
                     DREAMHRLAPNVKLIPGMPADVLIITEHRTMFDYLFQPFLEVFRRSFHEV"
     gene            1911..2126
                     /locus_tag="VFL49_01700"
     CDS             1911..2126
                     /locus_tag="VFL49_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910721.1"
                     /translation="MSVRIWATAATIACAALFAVMPWPSTSVEIPNDTAVVAIDFDAL
                     RAEAAAALRALEESREHRLASAPVTAF"
     gene            complement(2132..>2894)
                     /locus_tag="VFL49_01705"
     CDS             complement(2132..>2894)
                     /locus_tag="VFL49_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910722.1"
                     /translation="LSGHELTIGYPDHTVGRGLEVTLATGEVLALLGPNGGGKTTLLK
                     TLLGLLKAQAGEVRLDERPLDTYSIRDRARVVAYVPQVHIGTFAFTVETVVLMGRTAH
                     GNLFNRPSARDRAVAHSVLERFGIAALADRPYTMISGGERQLVLLARALAQEPQFIVL
                     DEPTASLDFGNQGKVMNEIRSLARSGHGVLFTTHDPNHALRAADRAYLLRDGQRIADG
                     PVGKVLDRDQLEALYRTTVDRIADSGSGAVAFLPG"
BASE COUNT          607 a          837 c          879 g          571 t
ORIGIN      
        1 gtcgcaaagc cgccaccccc gcaattcaaa gcctgccaac gcgctcggag acgcctacga
       61 gaacgctgac agatggctcc attgctatcc gtccaaaaca ggcggcccgg ctcgcgggga
      121 tctgccgcag caatttgttc cgcgcgattc gcgacggcca cttgcccgcc cgcaagtacg
      181 gccgctgcac gctcatcttg gtgcaggatc tttgggcctg gctggaacag ttgccgggaa
      241 tagccgaggg acgaaatcat caagcgggtt cataagcgtc ggaaagatac tcaggtagac
      301 gtacaatggc aggacatgaa gtcattctga aaccagctta tggaagcgtg gagattttcg
      361 ctcccgctcc cggctggcgt cgtccgtctc ccttgaaaac aagcatcgag ggtcccggtc
      421 gagtcggtct tgtgattatc cttgttttcg cggtcgtgtg cggagtttgg ggagttgtta
      481 ttcctcttgc gggaggagcg atggctcccg gtgctctggc gtctgatcag ggaaagaaag
      541 ttattcaaca tctcgaggat ggaattatcg ccgaacttcg cgtgcatgat ggcgacgtcg
      601 tggagagcgg acagcctctt gtcgtgctcg aaaacgtgca gacgcgggca acctatgatg
      661 cgttgcagca gcaaatgtgg tcgctgctcg ccaagcatgc acggcttcag gccgaaagcg
      721 agcatcggaa cataatagag tggccagctg agctgcatgc gagcaatgca aagatcgccg
      781 ccattctcaa cgctcagcgc ttggtattcg aaacgcgagc agccgagctt gcaaccaaga
      841 aaaatatttt gcgtcagcgc atcgatcaat tgaacgatca gatcagaggc ctcgaagccc
      901 agctggagag tgccgctggc cagggcgaat tgatcactga agaattgcag ggcaaggaag
      961 tgcttctgaa gcaggggttg atgcccaagc cggagtacct gagattaaag cgcaacaaag
     1021 tcgagattga cggcaggcac ggcgaatatc tcgcgcaaat cgccaaagcg aagcagcaga
     1081 tcggcgaaac gcagatgcag atcctgaatg cagacgccga acgggcgacc cagattgcaa
     1141 ccgagtccga cagcgtccgg attgacctct ccaatacgat ggagaagctg cggagcagtg
     1201 aagatgtcct caaacgaacg gtggtgactg cccctatcgg cggcaccgtc gtcaactcca
     1261 acttcaagac gattggcggt gtggttgaaa aaggccggcc gatcctcgaa atcgttccat
     1321 tgggcgacgc cttgattatc gaagcgcgtg ttacaccgca tgatgtcaag gccgtgcaca
     1381 atggactgaa tgctcaaatc cacttcaccg cctattcgag tcgctcgacg ccaaggattc
     1441 ccggaattgt tcagtccgta tctgccgatc gtcttttcga cgataagacg caccagccgt
     1501 attacctcgt caaagtaaac gtggaccggg aggcgatgca tcgcctggct cctaacgtca
     1561 aactcattcc cgggatgccc gccgatgtcc tgatcattac cgagcatcgc acaatgtttg
     1621 actacttgtt ccagccgttc cttgaggttt tccggcgcag cttccacgag gtctgaccgc
     1681 cgtcctaaaa ggcgaggccg attgccttgc cgcggacagt cgccgttcat tggccgtgct
     1741 cgcgaaataa gccaggaata tcaaggccaa ccggcgtttt tggccgccgg ccgcgggtta
     1801 aaatgtggct aacatgttgc gtccaagcgg gacgccgggc ttcgcgcggc ttcaatggct
     1861 gccacgtata aggctgtctc ggggggaggg gtcgagcagg aagcttgacc atgtctgtac
     1921 gaatttgggc caccgccgcc acgatcgcct gtgcggcttt gttcgccgta atgccgtggc
     1981 cgtcgacatc tgtagaaata ccgaatgaca cggctgtcgt tgcgatcgat ttcgacgccc
     2041 tgcgtgccga ggcggctgcg gcgctccggg ccctggagga gtcacgcgag caccgtctcg
     2101 cgtcagcgcc cgtcacagcc ttctgatcgt cctagcccgg caggaatgcc accgcgccgc
     2161 ttccggaatc ggcaatccgg tcgaccgtcg ttcgataaag cgcttccagc tggtcgcggt
     2221 cgagaacctt tcccaccggt ccatcggcga tacgctggcc gtcgcgcagc agataggcgc
     2281 ggtcggccgc gcgtagtgcg tgattaggat cgtgggtggt gaacagtacg ccatgtccgg
     2341 atctcgcaag gctgcgaatc tcgttcatca ctttgccctg gttgccgaag tcgaggctgg
     2401 cggtcggctc gtcgagcacg atgaattgcg gctcctgcgc cagcgcgcgc gccagcagca
     2461 ccagttgccg ttcgccgcct gagatcattg tgtaggggcg atcggcaagg gctgcgatgc
     2521 caaagcgctc caacacgctg tgcgcaaccg cacgatcacg cgcacttggc cggttgaaca
     2581 gattgccatg cgccgtgcgg cccatcagca cgacggtttc aacggtaaac gcaaaggtgc
     2641 cgatgtgcac ctggggaaca taggcgacga cgcgcgcgcg atcgcggatc gaataggtgt
     2701 cgagcggtct ttcgtcgagt cgcacctcgc ccgcttgcgc cttgagcaga ccgagcaagg
     2761 tcttgagcaa cgttgtcttt ccgccgccgt ttgggccaag cagcgcaagc acctcgccgg
     2821 ttgcgagtgt cacctcgagc ccgcggccga ccgtgtggtc ggggtaaccg atggtgagct
     2881 catggccgga gaga
//
LOCUS       DASSRL010000082         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_737111, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000082 DASSRL010000000
VERSION     DASSRL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737111"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1127
                     /gene="ispH"
                     /locus_tag="VFL49_01710"
                     /gene_synonym="lytB"
     CDS             132..1127
                     /gene="ispH"
                     /locus_tag="VFL49_01710"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7910723.1"
                     /translation="MKVILAKPRGFCAGVVRAIEIVERALDKYGPPVYVRHEIVHNKW
                     VVDALKARGARFVEELSEVPPNAITIFSAHGVAKKVEAEAAERGLRVLNATCPLVSKV
                     HAQGRHYLRQGRTVILIGHAGHPEVEGTVGQIPGPVLLVQTEEDVEALTLPEDTPIAY
                     VTQTTLSVDDTRGIIAALFRRFKDVVGPGTQDICYATQNRQTALLELTKLVDVIFVVG
                     AKNSSNSNRLCEIGVEAGIPTHLIASGSEIRPEWLHDVEVVGITAGASAPEVMVRDVI
                     NALRRITSVEVFTLPGQDEHIEFRVPVDLLDVTGGKSISKDINLGHSTSSETVCR"
     gene            1090..2232
                     /gene="hpnH"
                     /locus_tag="VFL49_01715"
     CDS             1090..2232
                     /gene="hpnH"
                     /locus_tag="VFL49_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET7910724.1"
                     /translation="MGIPLLQKLFVGSYVVRQRLTGQKRYPLVLMLEPLFRCNLACAG
                     CGKIDYPDPILHQNMSVEDALHAVDECGAPVVSIAGGEPLLHKQLPEIVRGIVARKKF
                     VYLCTNALLMEKKLSQYEPSTFFTWSVHLDGDKEMHDISVCQPGVYERAVAAIKAAKE
                     RGFRVNINCTLFNTAQADRVAEFFDTVTELGIDGITVSPGYAYERAPDQQHFLNRGKT
                     KQLFRDVFKRGPKNKKWSFSQSSMFLDFLAGNQNYHCTPWGNPTRTIFGWQKPCYLLG
                     EGYARTFKELMDGTDWDSYGTGNYEKCADCMVHSGYEASAVNDAVAHPLKALAVSLRG
                     PRTEGEMSPEISLDRQRPAEYVFSKHVERKMEEIKQAKKPELAKAG"
BASE COUNT          485 a          691 c          695 g          431 t
ORIGIN      
        1 gtcgcgaaag tattcgatgt cttcggagcg aagcatgctc cggaattgca gccatccgag
       61 atcgcaatcc ttcaggatcg cgcgcggcgt cggcggcaag cggccaatag ccggacgaac
      121 gggggggcgt gatgaaagtc attctggcga agccgcgcgg cttctgcgcg ggcgttgtac
      181 gtgcgatcga gattgtcgag cgcgcgctcg acaaatatgg accgccggtc tacgtgcgac
      241 acgaaatcgt gcacaacaaa tgggtcgtcg atgcgctgaa ggcgcgcggc gcccgtttcg
      301 tcgaagagct ttcggaagtg ccgccgaatg ccatcacgat cttcagtgcg catggcgtcg
      361 caaaaaaagt cgaggcggaa gcagccgagc gcggcctgcg tgtgttgaac gcgacgtgcc
      421 cgctggtgtc gaaggtccat gctcagggcc gtcactatct gcgacaggga cgtacggtca
      481 ttttgatcgg ccatgccggc catcctgaag tggaagggac ggtaggacaa attcccgggc
      541 cggtgctgct tgtgcagacc gaggaagatg tcgaggccct caccctcccc gaggacacgc
      601 cgatcgccta cgtgacgcag accacgctga gcgtcgacga cacgcgcggg atcattgccg
      661 ccctgttccg ccggtttaag gacgtcgtcg gccccggcac gcaggatatc tgctatgcga
      721 cacagaatcg ccaaaccgcc ttgctcgaac tgaccaagct ggtggacgtt atctttgtgg
      781 tgggagctaa gaacagttcc aattcgaacc gcctctgcga aatcggcgtc gaagcgggca
      841 ttcccaccca cctgatagca agcggttctg aaataaggcc ggagtggttg catgacgtgg
      901 aagtggtcgg tattacagcg ggcgcatcgg cgcctgaagt catggttcgg gacgtcatca
      961 acgctctgcg ccgtataacc tccgtcgaag tgttcaccct gcccggccag gacgagcaca
     1021 ttgaattccg cgttccggtc gacctgctgg atgtgaccgg cggcaaatca ataagcaagg
     1081 atatcaatct tgggcattcc acttcttcag aaactgtttg tcggtagtta cgtcgtccgt
     1141 cagcgtctga ccggccagaa gcgttacccg ctggtgctga tgcttgagcc gctgttccgg
     1201 tgcaacctgg cttgcgccgg ctgcggcaag atcgactacc ccgaccccat tctgcatcag
     1261 aatatgtcgg tcgaagacgc gttgcacgcc gtagacgaat gcggcgcgcc cgtcgtctcg
     1321 atcgccggcg gcgaaccgct tctgcacaag caattgcccg agatcgtgcg cggcatcgtc
     1381 gcgcggaaga aatttgtcta cctctgcacc aatgcgctgc tgatggaaaa gaagctgtcg
     1441 cagtacgagc ccagcacttt ctttacctgg tcggtgcatc tcgacggcga caaggagatg
     1501 cacgacatct ctgtctgcca gccgggcgtt tacgaacgcg cggtcgccgc aatcaaggcc
     1561 gccaaagagc gcggcttccg cgtgaacatc aactgcacct tgttcaacac cgcgcaggcc
     1621 gatcgcgtcg ccgaattttt cgacaccgtg accgagcttg gcatcgacgg catcaccgtg
     1681 tctcccggct acgcctatga gcgcgcgccc gaccagcagc acttcctcaa ccgcggcaag
     1741 accaagcagc tcttccgcga cgtgttcaag cgcgggccga agaacaagaa atggtcgttc
     1801 agtcagtcca gcatgttcct ggatttcctc gcaggcaatc aaaactatca ctgcacgccg
     1861 tggggcaatc cgacgcgcac catctttggc tggcagaagc cgtgctacct gctcggcgaa
     1921 ggatacgcca ggacattcaa ggagctgatg gacggcaccg actgggatag ctacggcacc
     1981 ggcaattacg agaagtgcgc cgactgcatg gtgcactcgg gctatgaagc ctcggccgtc
     2041 aacgacgccg tcgcgcaccc gttgaaagca ttggcggtca gcctgcgcgg accgcgcacc
     2101 gagggcgaaa tgtcgcccga gatttcgctc gaccgccaac gtccggccga atacgtgttc
     2161 tccaagcacg tcgagcgcaa aatggaagaa atcaagcagg ccaagaagcc ggagctcgcc
     2221 aaagctggct aaaagcgtgg gataaccgcg ctttcaatcc gttccggatg gaacttccgg
     2281 gcggatttgc ctgttgtgta cg
//
LOCUS       DASSRL010000083         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_964505, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000083 DASSRL010000000
VERSION     DASSRL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964505"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFL49_01720"
     CDS             complement(<1..693)
                     /locus_tag="VFL49_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910725.1"
                     /translation="MKSTLLMLAGGPLALRGALPIPEAQARDAGVGWRHGLSKLSDLK
                     YQAGFNHFDYVKANAPKGGAASLIVLGSFDNFNLVVGGAKGTLVSGVEFLYDTLMVPA
                     LDEVASIYGLLAEAVTYPDDFSSVTYRLRASAKWHDGKPVTPDDVVFSFNAFKKLSPQ
                     AAASFRQVTKAEKTAEREVTFHFDGAGNRELPQVVGQLTVLPKHWWEGTDGEGKKRSI
                     GDTTLEPPLGSGP"
     gene            complement(954..1079)
                     /locus_tag="VFL49_01725"
     CDS             complement(954..1079)
                     /locus_tag="VFL49_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910726.1"
                     /translation="MTKEEDVLAFEVSDEALEAAAGNEQVNYTLGACTGLSVCPG"
     gene            1451..>2593
                     /locus_tag="VFL49_01730"
     CDS             1451..>2593
                     /locus_tag="VFL49_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HET7910727.1"
                     /translation="MDFFSERLISRAATIDELLSDGFEAIPGDKDHAGVGQRRLAAWC
                     QSAAGGDWSLFERRLRRDGLDSAGVLARLTAVRRKPAVPTPRWVADATWILPALEATT
                     TSAAQESGDQTEPVAFEHLLAGVVENAEALLRSGVSDKATANLTASAHDCLRRALLVE
                     LSELAAPAFYERFSAMRDAAETVETAGLRQHSQASLYEKFVADMRTDGFRQLFEQKPV
                     LLRLLSTIVRQWIETSREFIMRLDADLPLIREVLHAGKNGGVSQLEGGFSDPHNEGRS
                     VRIVIFADGSKVVYKPKDLRVDVAWQGLVDRLNWAKPPVELKTARTIAYTGYGWTECI
                     EHAGCAAPDEFKIFFRRVGAWLALFHCFAANDMHQENIVATGSHPVP"
BASE COUNT          523 a          773 c          763 g          534 t
ORIGIN      
        1 gggcccgctg ccgagcggcg gctccagcgt cgtatcgccg atcgagcgtt tcttgccttc
       61 accgtccgtc ccctcccacc agtgcttcgg caggacggtg agttgaccga cgacttgcgg
      121 cagctcgcga ttgccggcac cgtcgaaatg gaaagtaact tcgcgctctg cggttttttc
      181 tgccttcgtg acttgccgaa aactcgcggc ggcctgcggg ctcaatttct tgaaagcgtt
      241 gaatgaaaag acgacatcgt cgggagtaac cggctttccg tcgtgccatt ttgcgctggc
      301 gcgcagtcga tatgtcaccg aagaaaaatc gtccggatag gtgacggctt cggcgaggag
      361 accgtagata ctggcaacct cgtcgagcgc gggcaccatg agcgtgtcgt aaaggaactc
      421 aacgccgctc accaaagtgc ctttcgcgcc tccaaccacg agattgaagt tgtcgaaact
      481 tccgagcacg atcagcgatg cagcgcctcc tttcggcgcg ttcgccttga cgtagtcgaa
      541 atggttgaaa ccggcttgat acttcaggtc cgaaagtttg gacaggccgt gccgccagcc
      601 gacaccggca tcacgcgcct gcgcttccgg tatcggcagc gcgcctctga gtgctagcgg
      661 ccctcccgcg agcatgagga gggtggattt catcaaggat cggcggtcca tcggcactcc
      721 agatactcgg ctcgcgcaat actgcattat gcgcgatcca cacggatttt gaaaacagca
      781 gaactcagcg tcaccggtca gcaccggcgg cgcccttctt cgtggacatg ccgagcggcc
      841 accgccagtg accggggttg aattgaaaaa taacgaggcg ccggatggac tatccgacgc
      901 ctcgtcagaa ttcagatcat caatcggccc tcgaaggacc tatcccacag tcctcagcct
      961 gggcagaccg agaggccggt gcaagcaccg agcgtgtagt tcacctgctc attgcccgca
     1021 gccgcttcca gcgcctcgtc ggagacttca aaagccagta cgtcttcttc ctttgtcatg
     1081 gcacaattcc tttcgtttgc cagcggcgcc ggcagtgaga aggtcgatta gaaaaccaga
     1141 acgccccccc agcgccatgg gcctatgatc cgatgcgagt tttacaatgt caattgcgaa
     1201 aagcctattc ctgcagttac ttatgaccgt tctacgacga aattccccaa gcgatggtgc
     1261 tgtctggttg cagatcatcg cggttaacgt ccgccagttg ctgccgatgc gccatatccg
     1321 tctgtcatcc gagcgaaatc gcacgcaaat tcaaaagcct cagccggggt ctcgcgatgc
     1381 agcaatggcc aggctgaccg gatattcggt gcccagtcgg ctataatcct tgggtactgt
     1441 gtgaggccgt atggatttct tctccgaacg tctgattagt cgtgcggcga caatcgacga
     1501 gctactgtca gacggcttcg aagccatccc cggagacaag gatcatgcgg gcgtcggtca
     1561 aaggcgcctg gccgcctggt gccagtctgc tgcgggcggc gattggtcgc tctttgagcg
     1621 caggcttaga cgagatgggc tcgattccgc cggcgttctc gcccggctga cggcggtccg
     1681 gcgaaagccg gccgtgccga caccgcggtg ggtggcggac gcgacatgga tcctgcctgc
     1741 tcttgaagca acaaccacct ccgcagccca agaatccgga gaccagacgg agccggtggc
     1801 attcgagcac cttttggccg gggtggttga aaacgcggaa gcgctgctgc gatcaggagt
     1861 aagcgacaaa gcaacggcaa acctcacggc atctgcgcat gattgcctgc ggcgcgcgct
     1921 tctcgtcgaa ctgtctgaac ttgccgcgcc ggcgttttat gagcgcttct cagcgatgcg
     1981 ggatgcggct gaaaccgttg agaccgcagg acttcgacag cattcgcagg cgtcgctcta
     2041 cgaaaaattc gttgccgaca tgaggaccga cggttttcgg cagctcttcg agcaaaagcc
     2101 tgtgctgctg cggctgctca gcaccatcgt ccgtcaatgg atcgagacct cgcgcgaatt
     2161 catcatgcgg cttgatgccg acctgccgct cattcgtgag gtcttgcatg ccggcaagaa
     2221 cggcggagtc agccagctcg aaggcggctt ctccgatccc cacaatgaag gccgttcggt
     2281 caggattgtc attttcgcag acggctcgaa ggtcgtttac aagccgaaag atttgcgcgt
     2341 cgatgtggcc tggcagggac tggtcgaccg ccttaactgg gcgaagcctc cggttgagct
     2401 taagacggcg cgtaccattg cctacaccgg atatggctgg accgaatgca ttgagcatgc
     2461 agggtgtgcg gcgccggatg aattcaagat atttttcagg cgtgtaggcg catggctcgc
     2521 gctctttcat tgcttcgccg ccaacgacat gcatcaggaa aacattgtcg ccacaggcag
     2581 ccatccggtg ccg
//
LOCUS       DASSRL010000084         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_334589, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000084 DASSRL010000000
VERSION     DASSRL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334589"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFL49_01735"
     CDS             complement(<1..405)
                     /locus_tag="VFL49_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910728.1"
                     /translation="MSARNRKYGMSRRTLLKAGAAISLLPAPQVFAQSPEPLKIGFLT
                     VLTGPLAAGGKQQEEGAALFLKERNGTIGGRKIELITQDTGGNPALAKTKLQELVERH
                     KVAVSIGPLATNEAIAMDNYVRDTKVPLITTTS"
     gene            complement(605..1060)
                     /locus_tag="VFL49_01740"
     CDS             complement(605..1060)
                     /locus_tag="VFL49_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7910729.1"
                     /translation="MPAQPAEESTGSVTAPGKKTAPGLLGSDPYDELNLGKKHYRANN
                     FGLAEKHFRLAVEKHPRDAEAWLGLAASYDRLRRFDLADRAYAQAIGILGPTVEILNN
                     QGYSYMLRGDYKRAHATLAAARRKDPNNKYVQNNLRLLEESYRKGKSVE"
     gene            complement(1376..2347)
                     /locus_tag="VFL49_01745"
     CDS             complement(1376..2347)
                     /locus_tag="VFL49_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7910730.1"
                     /translation="MDTVVTVFGNMLQDSNTMLMGLLVFLAAGTLAFSVMAAIRVRGS
                     VKRRTARILTDEERKANHARSLQHSSQKTVAKLIDYTTKHYGSANDENMKVLRRRLVQ
                     AGIYDPRGVAFFFIARTGLAVGLAFAVFLLLPLVKSSGGTFFWLMVIAGGVAGYVGPS
                     VYIDKRISAHKAEHRSGFPDFMDLLVVCADSGLSMEASLERVSRELGPSYPSLTANIH
                     LTNLEIRAGRPLKDALEHFADRLALEESKAFATLINQSIDLGSSITDAMRVYSDDMRH
                     KRLSAAEEKAYALPAKLAVPMMVCIFPVLFVVILLPVIVRLHMNGYF"
     gene            complement(2350..3315)
                     /locus_tag="VFL49_01750"
     CDS             complement(2350..3315)
                     /locus_tag="VFL49_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7910731.1"
                     /translation="MSNINSIYLFYIFIGLSAAMFAEGVYLLVYNNASYRKNINRRLK
                     VMSDKTDRESVLVQLRRERGLTSGGDYRLPLIKLNQLLLQSGLSIGLGRLILFVLLGM
                     VAMFAAVLFFHGTVSQAGMVTLVGGVVLPPMVLKILRGRRWKKFGAQFPDAIDIIVRS
                     LRAGHPVPIAITMVGKEMPDPIGSEFGIVSDEMTYGSDLETAMRNLYFRVGTDDLPLF
                     VTAVAIQRSTGGNLGEILENLSSVIRDRFKMRRKIRALAAEGRASALILSSLPIGMFA
                     IIQFLVPSFYAGVWDQNLTKIALTLAGCWMSVGNFIMYRMVNFRI"
     gene            complement(3322..>3927)
                     /locus_tag="VFL49_01755"
     CDS             complement(3322..>3927)
                     /locus_tag="VFL49_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7910732.1"
                     /translation="KERLLTIEDAAELQLQQPHVGRMETRPPNIEGKGEIRQRELVKN
                     ALRMRPDRIILGECRGEEAFDMLQAMNTGHEGSMATIHANTPRDAISRLEQMIGMAGL
                     PMTVASIRSQIANAIQLIVQLQRQSDGKRKVTSIAEVTGLEGDIIQMQEIFKFVRSGT
                     APDGAVQGYFQATGVRPRFLSHLTNLGITVPGSYFDPSKQL"
BASE COUNT          819 a         1119 c         1183 g          806 t
ORIGIN      
        1 cgatgtcgtg gtgattagcg gcaccttggt atcgcggaca tagttgtcca tcgctatggc
       61 ttcgtttgtc gccagcggac cgatcgacac ggccaccttg tggcgctcga ccagttcctg
      121 cagcttggtc ttggcaagcg cagggttgcc gccggtgtcc tgagtgatca actcgatctt
      181 gcggccgcca atcgtcccgt tgcgttcctt gaggaacagc gccgcgcctt cttcctgctg
      241 cttgccgccg gcggcgagcg ggccggtgag aaccgtcaga aagcctattt tgagcggctc
      301 cggagattgc gcaaagacct gcggcgcggg aagcaagctg atcgccgcgc ctgccttcag
      361 cagcgtgcgg cgtgacattc catatttccg gttgcgtgcg gacatcgccg gtccctcccc
      421 gtttgtctga ggtttccttt gtgatggcaa atcgattgag cgatagcggc tttttgagct
      481 cggcctgtca gctgagcctg atgggcctta tgcccgatca acaccgattg tgggggaata
      541 ttcgcatatc cggcgatttc cgccaccacc cccgcgggca atagcggcac agggcttggc
      601 cgccctattc gacagacttg ccttttcggt agctctcttc caggaggcgt aaattgttct
      661 ggacgtattt gttgtttgga tccttgcgcc tggcggcggc gagcgtcgcg tgcgcgcgct
      721 tatagtcgcc gcgcagcatg tatgagtagc cctggttgtt gagaatctcg accgtcggcc
      781 cgaggatacc gatggcctgc gcataggcgc ggtcggcgag atcaaagcgg cgcagacggt
      841 cataagaggc cgcgagtcct aaccaggctt ctgcgtcgcg cggatgcttc tccaccgcca
      901 ggcggaagtg cttttcagca agcccgaaat tattggcgcg atagtgcttc ttgccgaggt
      961 tcaattcgtc gtacggatcg ctgccaagca ggccgggtgc ggttttcttg ccaggagccg
     1021 tgacagagcc agtcgattcc tcggctggtt gcgctggcat cgatccgtcc accgtcaaat
     1081 gcggttcagt cgtctcgtcg acctgagcag atgcagatgc ttcagcaccc ggtgtttttg
     1141 gtccaaacag gttcgaaaag ctcgaggagg tgctgcatcc agccagccac aaaccagcgg
     1201 cggcgatgac cagcggtcgg gcaattccaa gaagcatttc aaagccttgc ggaaacgccc
     1261 gaataacggg acaccgtcag gctaggggag gatggttaag aattcttcac tttctcggaa
     1321 cttccgaatt tcgtggcgaa catggttcca accggccccg ctctcgccgt tggcactaga
     1381 aatagccgtt catgtgcagt cgcacgatca ccggcaggag aatgaccacg aacagaaccg
     1441 gaaagatgca gaccatcatc ggcaccgcaa gcttggccgg tagcgcatag gccttttctt
     1501 ccgcggcgga gagccgtttg tggcgcatgt cgtcgctgta gacccgcatt gcatctgtga
     1561 tgctggagcc gagatcgatc gactgattga tcaaggtggc gaaggccttc gactcttcga
     1621 gtgcgagacg gtcggcgaag tgctccagtg cgtccttgag cggccggccg gcgcgaattt
     1681 cgaggttggt caagtgaata ttggcggtaa gcgacggata gcttggcccc aactcgcgcg
     1741 agacgcgctc gagcgaggcc tccatcgaga ggcccgagtc cgcacatacg acaagcaaat
     1801 ccatgaaatc cggaaaaccc gagcgatgct cggctttgtg tgccgaaatg cgtttgtcga
     1861 tgtagacgct cgggccgacg tagccggcaa cgccgccggc gatcaccatc agccaaaaaa
     1921 aagtgccgcc tgaggacttc accagcggca aaagcaggaa gaccgcaaag gcgagaccga
     1981 ccgccagacc cgtacgcgca ataaaaaaga aagcaacacc acggggatcg tagataccgg
     2041 cttgtaccag ccgccgacgc aacaccttca tgttctcgtc gttcgccgat ccgtagtgct
     2101 tcgtcgtgta atcgatcagc ttggcgacgg ttttctggct ggaatgctgc agcgatcgcg
     2161 catgattggc cttgcgctcc tcatcggtca ggatgcgcgc agtgcgccgc ttgacggaac
     2221 cacggacgcg aattgccgcc atcacggaga aagccaaggt gccggcagca aggaagacta
     2281 gcagccccat cagcatggtg ttgctgtctt gcagcatatt gccgaatacg gtgaccactg
     2341 tatccatggt cagatcctga agttgaccat gcgatacatg atgaagttgc cgacgctcat
     2401 ccagcacccc gcaagggtga gcgcgatttt cgtcaggttt tgatcccaga cgcctgcgta
     2461 gaagctgggg acgaggaact gaatgatggc gaacatgccg atcggcagcg aagaaagaat
     2521 cagcgcggaa gcgcggcctt cggcggccag tgcgcgaatc ttgcggcgca tcttgaagcg
     2581 atcacggatg accgacgaaa ggttttcgag aatttcgccc aggttaccac ctgtcgaccg
     2641 ctgaattgcc acagcggtga cgaacagagg caagtcatcg gtgcccacgc ggaaatacag
     2701 attgcgcatc gcggtttcga gatcggagcc gtaggtcatt tcgtccgaga cgatgccgaa
     2761 ctccgagccg atcggatccg gcatttcttt tccgaccatg gtgattgcga tgggcaccgg
     2821 atggccggcg cgcaaggatc ggacgataat gtcgatcgca tcgggaaact gcgcgccaaa
     2881 ctttttccag cgtcggccgc gcagtatctt cagcaccatc ggcggcagaa ccacgccgcc
     2941 gacaagggtg accattcccg cttgtgatac tgtgccgtga aaaaagagaa cggccgcaaa
     3001 catcgcgacc atgccgagca acacgaagag gatcagacgg ccgagaccga tggaaaggcc
     3061 cgactgcagc agcaactgat tgagtttgat caatggcagc cggtagtcgc caccgctggt
     3121 caaaccgcgc tcgcgccgca attgcacgag cacgctttct cggtcggttt tgtccgacat
     3181 aaccttgagg cggcgattga tgttcttgcg gtaggacgca ttgttgtaaa caagcagata
     3241 gacaccctcg gcaaacatcg cggccgagag gccgatgaag atatagaaca ggtaaatcga
     3301 attaatgttg gacatgaccg gtcagagctg cttcgaggga tcgaaatagc tgccgggaac
     3361 agtgatcccc aaattcgtaa ggtgagaaag aaagcgcggg cgcacgcctg tcgcctggaa
     3421 atatccttgc acggccccat cgggggcggt gccggatcgc acgaacttga agatttcctg
     3481 catctgaatg atgtcgccct cgagtccggt cacttcggca atcgaggtta ccttgcgttt
     3541 gccatccgac tggcgctgta gctggacgat aagctggatg gcattcgcaa tctggctgcg
     3601 gatcgatgca acggtcattg gcaagcccgc catgccgatc atctgctcaa gacgcgagat
     3661 tgcgtcacgc ggcgtattgg cgtggatcgt cgccatcgag ccttcgtgac ccgtgttcat
     3721 cgcctgcagc atgtcgaagg cttcttcacc acggcactca ccgaggatga tgcggtcggg
     3781 acgcatacgc agcgcgttct tcacgagttc gcgctgacgg atttcgcctt tgccttcgat
     3841 attcggcggg cgcgtttcca tgcggccgac gtgcggctgc tgcagctgca attcggcggc
     3901 gtcttcaatc gtcagcagtc gctcctt
//
LOCUS       DASSRL010000085         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1349417, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000085 DASSRL010000000
VERSION     DASSRL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349417"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..212)
                     /locus_tag="VFL49_01760"
     CDS             complement(54..212)
                     /locus_tag="VFL49_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910733.1"
                     /translation="METLKEAKVFGLSYDDACARALPGSMEAYHLWLKNLHDPRGEAA
                     TLQACETD"
     gene            407..577
                     /locus_tag="VFL49_01765"
     CDS             407..577
                     /locus_tag="VFL49_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910734.1"
                     /translation="MAVAGSTVRAGRSKKTRRKNGGRIGRRLILGAVSIASHIAAIIG
                     TAVIIIDLVHHW"
     gene            816..1490
                     /locus_tag="VFL49_01770"
     CDS             816..1490
                     /locus_tag="VFL49_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7910735.1"
                     /translation="MDLINTISVWLLPLLFAITFHEAAHAYVARFLGDHTASQLGRVS
                     LNPLRHIDPFGTVLLPGLLLLARSPFLFGYAKPVPVNFRALRHPRMGMVLVAAAGPAM
                     NIALAVAAALSFHLVVYLPATAAQWTALNLKNAVVINAILAVFNLFPLPPLDGGRIAV
                     GLLPNALGRRLARLEPYGMAILIGLIIVLPLLGTQFGVDLGFVSHAITAATGAVIRMI
                     MIATGA"
     gene            1716..>2245
                     /locus_tag="VFL49_01775"
     CDS             1716..>2245
                     /locus_tag="VFL49_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7910736.1"
                     /translation="MIMRKVLAALVALLFVGYAGAASAQQKVRMQTAVPSASIYFELL
                     KRYADRVDKMSNGRVKIEVLPDGAIVPAFEILDAVDKGIVEAGYAWTHYWSGKHPAAG
                     IFSNPMAGAGAGLDQMSHVAWLLEGGGNQLLDRFYAEILKVNVVPLMVQPMGPDPLGW
                     FKRPIKDVADFKTMKYR"
BASE COUNT          402 a          678 c          708 g          457 t
ORIGIN      
        1 cgcgaggaca attgcgttcc gccgctgtag gcaggtcttg cgcacgtttt gcatcaatcg
       61 gtctcacaag cctgcaaggt ggcggcctct ccgcgcgggt catgcaggtt cttgagccac
      121 agatggtagg cttccatgct cccgggcagc gccctggcgc aggcgtcgtc gtaggacagc
      181 ccgaagacct ttgcctcctt aagggtctcc acacgagccg gcgcaaccgg ttcgcgtcct
      241 catgggagca gacaaccatg gggatattat cctataggaa tattgtcaag agacggcatg
      301 tggaaaacaa tggcgcgaga gtccaggcac catgcgccgc aagcacaaac gacaagccgc
      361 agtccgcata aagcggccca ttcgccaaaa tgcatgcggt cacggcgtgg ccgttgctgg
      421 gtccacagtt cgtgcaggca gaagtaaaaa gacgcgacgc aagaacggag gcaggatcgg
      481 gcgccggctg atactcggcg cggtatcgat cgcctcacac atcgccgcga tcatcggcac
      541 cgccgtcatc atcatcgacc tggtgcatca ttggtgactt gacggttcgc cacggttttg
      601 cgttacgccg taagatggga tgagcgagca aaacccatcg cctcatacat cgccccgaat
      661 ttcgcggagc ttgtcatcgg gccggccact tcgggccgga cccgttggct cggctacgaa
      721 gaacgggagg gaacgcgaga tcggcgagtt cgtggtaatc ggcgcctgat atattgccgc
      781 cgctgagtcg atgactgttt ctgatcgccc gcatcatgga cctgatcaac acaatttccg
      841 tctggctgct gccgctgctg tttgccatca cgttccacga ggcggcgcac gcttacgtcg
      901 cgcgatttct gggcgatcac acggcgtcgc agctggggcg tgtcagcctc aatccgctcc
      961 ggcatatcga tccgttcggc acggttcttc tgcccgggct gctcctgctg gcgcgctcgc
     1021 cgtttttgtt cggctacgcg aagccggtgc cggtcaattt tcgcgctttg cgtcacccgc
     1081 gcatgggaat ggtcctggtc gcggcggccg gtccggcgat gaacatcgcg ctggccgtcg
     1141 ccgctgcgct gagcttccac ctcgtggttt atctgccggc gaccgcagcc caatggacgg
     1201 cgctcaatct gaagaacgcc gtcgtcatca acgcgatcct ggcggtgttc aatctgtttc
     1261 cgctgccgcc gctggatggc gggcgcatcg cggtgggatt gcttcccaat gcgttggggc
     1321 gccggctggc gcggctcgag ccttacggca tggcgatcct gatcggattg atcattgtgt
     1381 tgccgttgct gggaacgcaa ttcggcgtcg accttggatt tgtgtcgcac gccatcacgg
     1441 ccgcaaccgg ggccgtcatc cggatgatca tgatcgcgac cggcgcatag gatgggttga
     1501 gcgccagcga aacccatcgc tcgatgatcg ggagaccatc ggcacatcat ttaagtcctg
     1561 ggtttcgctt tcgctcaacc caggccacgg cgtaaccaca gcagcccaat tcccgcggcg
     1621 tgagacacaa cagttcattt gtgtcttgaa cgagttcgct tgcgtgtctc gaccgaaagt
     1681 aggaggcttt agcctcgctt acggagggcg actacatgat aatgcggaaa gtactggcgg
     1741 cgctcgttgc gctgcttttc gtcggttacg caggcgccgc ctcggcgcag cagaaggtgc
     1801 ggatgcagac cgcggtgccg tctgccagca tctatttcga gctgctcaag cgttacgccg
     1861 accgtgtcga caagatgtcg aacgggcggg tgaagatcga ggtccttccg gacggcgcga
     1921 tcgtgccggc gttcgaaatt cttgacgccg tggacaaggg catcgtggag gcgggctacg
     1981 cctggacgca ctactggtcc ggcaagcacc cggccgccgg catcttctcc aatccgatgg
     2041 cgggcgcggg cgcgggcctc gaccagatgt cccacgtggc gtggctgctg gagggtggcg
     2101 gcaaccagct tttggaccgt ttctacgccg agattctcaa ggtcaatgtc gtgccgctga
     2161 tggtgcagcc gatggggccg gatccgctcg gctggttcaa gcggccgatc aaggacgtcg
     2221 ccgacttcaa gaccatgaag tatcg
//
LOCUS       DASSRL010000086        10494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_614631, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000086 DASSRL010000000
VERSION     DASSRL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614631"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..900)
                     /locus_tag="VFL49_01780"
     CDS             complement(154..900)
                     /locus_tag="VFL49_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7910737.1"
                     /translation="MAGHSQFKNIMHKKGRADKARSKLFGKLAREITVAAKLGMPDPA
                     FNPRLRAAILAARAENMPKDNIERAIKKSQGADTESYDEIRYEGYGPGGVAVIVEALT
                     DNRNRTAGEVRAIFSKNGGNLAETGAVSFMFSHVGVVEYDAKVASADAMLEAAIEAGA
                     EDVSSDENGHQIIATPDSLNEVAKALEVKFGEPRKSAMLWKPQNTIALDDESGEKILK
                     LIESLDDSDDVQNVYANFEVSDALMQKMSA"
     gene            complement(995..2353)
                     /locus_tag="VFL49_01785"
     CDS             complement(995..2353)
                     /locus_tag="VFL49_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7910738.1"
                     /translation="MCWVRQRDLGTEHMALYVNSNLALELPADLDRQSISSRAKADTR
                     AELAAQFIPAGARVLDLGANSALQTMLPNGCSYVRAGKKLRANDVDEFPTDAASRSDV
                     IVMLGMLERVKDLESLFTHLRFCKRDIVLSCYPTDFTSGARERDFVNRMSLCDLALLF
                     DRYGFRIESTAPVDESQMLMRLTPAERLAPVAPWNVAVISDGDTNDLAARLGRQLIHT
                     ILPGECRVHRLTPGMLDQAQPNYDLVILGAGSGLFPPLLGEGVLDVIARAKSAVGIFG
                     TQGRELIARPAFERLLDRLDTWFARYEDDVLTFGRGRNNVIHTGDWLIDQFPLARATN
                     DEPLVINGDLGPDFALDRAISTIQQYRQVYSTVPVALLCALTSADLAAYAETPAQQPD
                     LSAGQFRSMLIDIFGRTWPQQKFFLVDRDAVIRYKTRVHQNVSKIGARIGAILRNVAI
                     AA"
     gene            complement(2423..3244)
                     /locus_tag="VFL49_01790"
     CDS             complement(2423..3244)
                     /locus_tag="VFL49_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET7910739.1"
                     /translation="MRILFIGDIVGRSGRTIVIDRLPTLISEWKLDLVIVNGENAAGG
                     FGITEVIYNELVDAGADAITLGNHAWDQREALVFIERAPRLVRPLNFPKGTPGRGAAL
                     VDTKNGKRALVINAMGRIFMDPLDDPFAAIERELSACALKSGADAIVIDFHCEATSEK
                     QAMGYFTDGRASLVVGTHTHAPTADDRILPAGTAFVSDVGMTADYDSIIGMNKDEPLN
                     RFLRKLPGAKFEASSGPATLCGLAVETDDATGLARKVSAVRLGGILQEARPGFWI"
     gene            complement(3248..3856)
                     /locus_tag="VFL49_01795"
     CDS             complement(3248..3856)
                     /locus_tag="VFL49_01795"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7910740.1"
                     /translation="MTEQVVSASDTKSTDKSQLRAEAFARRDSLPADERAAAAEAIAA
                     RSFPVAISAGMIVSGFSPVKTEINPLPLMRKAAHLGARLALPAIAGRGKPLIMRAYAF
                     GDALARGQWGIREPKSDAPEVAPDILIVPLAAFDRTGHRIGYGAGYYDMTINALRAKK
                     KVIAMGIAFAAQEIVSVPATERDARLDFVLTEREVIDLRESN"
     gene            complement(4033..4190)
                     /gene="ssrS"
                     /locus_tag="VFL49_01800"
     ncRNA           complement(4033..4190)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFL49_01800"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4242..4631)
                     /locus_tag="VFL49_01805"
     CDS             complement(4242..4631)
                     /locus_tag="VFL49_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7910741.1"
                     /translation="MAQVNATIAGRQFRLACEDGQEEQLQALAHDIDQRIIDLRRKFG
                     EIGDTRLTVMAALMLADELVEVTQRLRRLDEEIAALHDARVVAADRAKAASDAVVGAF
                     NSAAERIEGITKKLNQTVGNGGSVAIG"
     gene            complement(4637..4915)
                     /locus_tag="VFL49_01810"
     CDS             complement(4637..4915)
                     /locus_tag="VFL49_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HET7910742.1"
                     /translation="MSEESAIDAAVKRLALALDALDAAVERRKQADRNEEGLAAQVQA
                     LGIDRTRLAAALDGEAARSRRLETTNREIAERLDAAITSIQSVVDLHE"
     gene            5203..7170
                     /gene="tkt"
                     /locus_tag="VFL49_01815"
     CDS             5203..7170
                     /gene="tkt"
                     /locus_tag="VFL49_01815"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7910743.1"
                     /translation="MANAIRALAMDAVEQAKSGHPGMPMGMADAATILFTRFLKFDPA
                     DPDWSDRDRFVLSAGHGSMLIYALLYLTGYAAVTIDQIKRFRQLGSNTAGHPEHGHTP
                     GVETTTGPLGQGLANAVGMAIAEKHLAAVFGDGIVNHRTYVIASDGDLMEGISQEAIA
                     LAGHLKLDKLIVLFDDNGISIDGALSLTDSVDQLKRFEAAGWNASRIDGHDPAAIASA
                     LEKAQRSDKPVLIACRTKIGFGAPTKEGSEKSHGSPLGADEISGARKTLGWTSAPFEV
                     PADILKAWRTAGARSQAVHADWKKRLAALESGKRAEFDRRMSGKIPQQALGDAIRAVK
                     EKLAAQPKDIATRTACEFALESLIPALPEMIGGSADLTGSNNTRTKSMKAITAADFAG
                     RFIHYGVREHGMAAAMNGMALHGGIIPYSGTFLVFSDYCRPAIRLAALMGKRVIHVMT
                     HDSIGLGEDGPTHQPVEHLAALRAIPNLLVFRPCDAMETAECWQLALASENRPSILAL
                     TRQNLPPLRNRFDAENICARGAYEIAGPDGKADVSIFATGSEVSVAVDAAKLLAERGI
                     AARVVAMPCFELFLEQSEDYRRAIIGSARIRIGVEAAVRQGWDAVIGSDGLFVGMNSF
                     GASAPYKELYKKFGITAEAVVAATLSKLGKM"
     gene            7219..8226
                     /gene="gap"
                     /locus_tag="VFL49_01820"
     CDS             7219..8226
                     /gene="gap"
                     /locus_tag="VFL49_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7910744.1"
                     /translation="MTVRVGINGFGRIGRNILRAIAEAGRKDIEVVAVNDLGPVETNA
                     HLLRYDSVHGRFPGKVTVKGDMISVGNGSIKVTAERDPAKLPWKDLGVDIAMECTGIF
                     TAKEKASAHLTAGAKRVLVSAPADGADFTVVFGVNHDKLTRDHKVVSNGSCTTNCLAP
                     VAKVLHDAVGIESGFMTTIHAYTGDQPTLDTLHKDLYRARAAAMSMIPTSTGAAKAIG
                     LVLPELKGKLDGVSIRVPTPNVSVIDLKIVARKPTTKEEINDAVKRAAEQQLKGILSY
                     TNDPNVSIDFNHDPHSSTFHMDQTKVINGTLVRVMSWYDNEWGFSNRMADTAVAMGKL
                     L"
     gene            8345..9538
                     /locus_tag="VFL49_01825"
     CDS             8345..9538
                     /locus_tag="VFL49_01825"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7910745.1"
                     /translation="MEKFRTLDRVDVKGKRILLRVDLNVPVENGVVTDTTRIERAAPA
                     ITELADKGGKVILLSHFGRPKGRDPKLSLKPVAAEVARIIKRPVRFADDCIGEAAGRA
                     VGAMKPGDIVCLENTRFHPGEEKNDPAFVAELAKLGDIWVNDAFSAAHRAHASTEGLG
                     HKLPAYAGRTMQAELEALSGALEHPERPLAAIVGGAKISTKLDLLGNLLDKVDTLIIG
                     GAMANTFLLAQGKTVGKSLVEADLVGTARDIMAKATTAKREIVLPVDAVVAQKFEAQA
                     PSRVVDVDNVDAADMILDIGPRSIEQVVSVLARAKTLVWNGPFGAFEMEPFDNGTVEV
                     AEAAAELSEAGKLVSVAGGGDTVAALNVAGVTDRLTYVSTAGGAFLEWLEGKALPGVE
                     VLRVK"
     gene            9588..>10494
                     /locus_tag="VFL49_01830"
     CDS             9588..>10494
                     /locus_tag="VFL49_01830"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET7910746.1"
                     /translation="MNLDELNKVAQAMVASGKGILAADESSGTIKKRFDAIGVESTED
                     ARRDYREMLFRSAEGMKHISGVILYDETIWQKAKDGTPLVNLIRKAGSIPGIKVDEGT
                     KPLPECPGELVTVGLDRLADRLPKYYEQGARFAKWRGVIDIGPGIPTWTCVLANAHAL
                     ARYAALCQAAQIVPIVEPEVLMDGDHDIDRCYEVTEFVLKETFQQLYYQKVALEGIVL
                     KPNMAVAGKKSAKKAGVQEVAEKTVRLLKNCVPGAVPGIAFLSGGQSDEDATAHLDAM
                     NRRGPHPWQLSFSYARAIQNVAMERW"
BASE COUNT         2131 a         3230 c         3198 g         1935 t
ORIGIN      
        1 gtgccgccgc agccaagaac tctcgccgac acccgcccga accgcgcacg cgtcattcag
       61 tcggcggatg ttttcgtcga cgccagcggg ctgatctacg caaccgacta caatgccgga
      121 ctttatattc tggaattcaa cggcgcgtag tgctcacgcg ctcatcttct gcatgagcgc
      181 atcggacacc tcaaaattcg cgtaaacgtt ctgcacgtcg tcgctgtcgt cgagcgactc
      241 gatcagcttg aggattttct cgccggactc gtcatcaagc gcaatcgtgt tttgcggctt
      301 ccagagcatc gccgatttgc ggggctcgcc aaacttgacc tcgagcgcct tggcgacttc
      361 gttcaacgag tccggcgtcg caatgatctg gtgtccattc tcgtccgagc ttacgtcctc
      421 ggcgccggct tcgattgccg cttccagcat cgcgtcagcg ctggcaacct tggcatcata
      481 ctcgaccacg ccgacgtggc tgaacatgaa ggatacggcg ccggtctcgg cgaggtttcc
      541 gccgttcttc gagaagatcg cgcgcacttc acctgcggtg cgattccggt tgtcggtcaa
      601 cgcctccacg atgaccgcca cgccacccgg tccgtagccc tcgtagcgaa tttcatcgta
      661 gctttcggta tcggcgccct gcgatttctt gatagcgcgc tcgatattgt ccttcggcat
      721 gttctcggcg cgggctgcca gaattgcggc gcgcagtcgc ggattgaacg ccggatccgg
      781 catgccaagt ttggccgcaa cggtaatttc gcgcgcaagc ttgccgaaca gcttggagcg
      841 cgccttatct gcgcgcccct ttttgtgcat gatattcttg aattgggaat gaccagccat
      901 aaccagcgtc aatattgtgg gtgccggcgg ctatatagtc cggcgcgtcc ggaaaataaa
      961 tatcgtagat cgctggactt tgcgggcgag ccaatcaggc ggcaattgcg acgtttcgca
     1021 ggatagcgcc gatgcgtgcg ccgattttcg atacgttctg gtggacgcgc gtcttgtagc
     1081 ggatgacggc atcccgatcg acaaggaaga acttctgctg aggccatgtg cggccaaaaa
     1141 tgtcgatgag catgctgcgg aactgacctg ccgaaaggtc gggctgctgc gccggcgtct
     1201 ccgcataggc cgcaagatca gccgaggtga gcgcgcataa aagggccacc ggcacggtcg
     1261 aatagacctg cctgtactgc tgaatggtgc tgatcgcgcg gtcgagggcg aaatcggggc
     1321 cgagatcgcc gttgatcacc aaaggctcgt catttgtagc gcgggcaagc ggaaactggt
     1381 cgataagcca gtccccggta tgaatgacat tgttacggcc acgcccgaac gtcagcacat
     1441 catcttcata acgcgcgaac cacgtatcaa gtcgatcaag gagacgctcg aaagccggcc
     1501 gggcaatcag ttcccggccc tgcgtgccga agattccgac ggcagatttc gcgcgggcaa
     1561 tgacatcgag gacaccttcg ccaagaagag gcggaaacag gccgctgcca gcaccgagga
     1621 taacgagatc gtagttcggc tgcgcctgat caagcatccc cggggtcaga cgatgaaccc
     1681 ggcattcgcc aggcaatatc gtatggatca gctgccggcc cagccgcgcg gcgagatcgt
     1741 tagtatcgcc gtctgaaatc acggccacat tccacggcgc aaccggagca agccgctccg
     1801 cgggcgtcag ccgcatgagc atctgcgatt catcgaccgg ggcggtgctt tcgatacgga
     1861 agccgtaacg atcgaacaac agcgccaggt cgcagagact cattcgattg acaaaatccc
     1921 gctctcgcgc gccgctggtg aaatccgtcg gataacagct gaggacgatg tcgcgcttgc
     1981 agaagcgcaa atgagtgaac aggctttcca ggtctttgac acgctccagc atgccgagca
     2041 tcacaatgac gtcggaccgc gatgcagcgt cggtggggaa ttcgtcgacg tcgttcgccc
     2101 gcagtttttt gccggcgcgg acatagctgc agccattcgg caacatagtc tgcaatgctg
     2161 agttcgcccc gagatcgaga acacgcgccc cggcgggaat gaactgcgcc gcaagctcgg
     2221 cgcgggtgtc ggccttggcc cgcgatgaga tcgactggcg gtcgaggtcc gccggaagct
     2281 ccagcgcaag attgctgttg acgtagagag ccatatgctc cgtgccgagg tcgcgctgcc
     2341 ggacccagca catgccggcg aatcaattcc gtctaccgaa tcatcgcggc gattcgcggt
     2401 caactgcgca gattgaaaaa agtcagatcc agaagcccgg ccgggcttcc tgcaggatgc
     2461 cgccgaggcg cacggcactg accttgcgcg cgagccctgt cgcatcgtcg gtctcgacgg
     2521 cgaggccaca cagggtcgcc ggcccgctcg acgcttcgaa cttcgcgccg ggcaacttgc
     2581 gcaggaagcg attaagcggc tcatccttgt tcatgccgat gatggagtcg tagtcggccg
     2641 tcatcccgac gtcggacacg aacgccgttc ctgccggaag aatgcggtca tctgcggtgg
     2701 gagcatgcgt atgggttccc acgaccagac tggcgcgccc gtcggtgaaa tagcccatcg
     2761 cctgcttttc gctggtcgct tcgcaatgaa agtcgatgac aattgcatcc gcgccggatt
     2821 tgagcgcgca cgccgaaagc tcgcgctcga tcgccgcaaa agggtcgtcg agcggatcca
     2881 tgaagatccg gcccatggcg ttgatcacca gagcccgctt gccgttcttg gtatcgacca
     2941 gcgcagcacc gcgaccaggc gtgcctttcg gaaaattcag cggccgaacg agcctgggtg
     3001 cgcgctcgat gaaaaccagc gcctcgcgct gatcccacgc gtgattgccg agcgtgatcg
     3061 catcggcgcc ggcatcaaca agctcgttat agatgacttc agtgatgcca aaaccgccag
     3121 ccgcgttctc gccgttgact atgacgagat cgagcttcca ttcggaaatg agcgtgggca
     3181 gacgatcgat cacgatcgtg cgcccggagc ggccgacaat atcgccgata aaaagaatac
     3241 gcatgcgcta attcgactcg cgcaaatcga taacttcacg ctccgttagc acgaaatcga
     3301 ggcgcgcgtc gcgttcggtc gcggggacgg aaactatctc ctgcgccgca aaagcaatac
     3361 ccatggcaat gactttcttc ttggcccgca gcgcattgat ggtcatgtca tagtagccgg
     3421 cgccatatcc gatgcgatga ccggttcggt cgaatgcagc aagcgggacg ataagaatat
     3481 cgggggcgac ttcaggcgcg tcggattttg gttcacggat gccccattgc ccacgcgcaa
     3541 gcgcgtcgcc gaaagcgtag gcccgcatga tcagcggctt gccacgtccc gcgatcgctg
     3601 gaagcgcgag acgcgcgccg agatgagcgg ctttgcgcat caaaggaaga ggattgatct
     3661 ccgtcttcac cggcgagaat ccggacacga tcatgcccgc cgaaatggcc accggaaacg
     3721 accgggctgc gatcgcctca gctgcagcgg cgcgctcatc cgccggcaaa gagtcgcgcc
     3781 gtgcgaaggc ctcggcgcgc agttgggatt tgtcggtgga cttggtatcg gacgcgctca
     3841 ccacttgctc cgtcatgccc ggccttgtgc cgggcatcca cgtcttccga atctggcata
     3901 attaaagacg tggatggcac gagcaactcg ggcctgcccg agttgctcag aaattaatat
     3961 tcgctgcaag tcgggtatac ccgacttgcg gtgacaagcc cgccatgacg attttgatac
     4021 gcagtgctca agtgcggagc cgcgatggcc gttggagcat cgatcccggg ttccctacga
     4081 aagtaggtgg gcgccatatg accgaaccca cgagcccggc cagggacagc tcccttaagg
     4141 atcgataagg ccccggggat tgtgtctcct gacgcacccc gcagccccgc cggcagaatg
     4201 tagtggttat ggcaaagggg cgccactcgt cgcgaagagg gtcagccaat ggcgacgctg
     4261 ccgccattgc ccacggtttg attcagtttt ttggtgatgc cctcaatccg ctctgcagcc
     4321 gagttgaatg cgccgacgac ggcatcggat gcagcctttg cgcggtcggc cgcaacaacg
     4381 cgggcatcgt gcaaggcggc gatctcctcg tccaggcgcc gcagtctttg cgttacttcg
     4441 acaagttcgt cggcaagcat cagcgccgcc atgaccgtca gacgcgtatc gccgatctct
     4501 ccaaacttgc gccgcaggtc gatgatgcgc tgatcaatgt cgtgcgcaag cgcctgcagc
     4561 tgctcttcct gcccgtcctc gcaggcgagg cggaattgtc gaccggcaat cgtggcgttg
     4621 acctgtgcca tggttgtcac tcgtgcaaat ccaccaccga ctgaatggac gtaatcgccg
     4681 catccaaccg ttccgcaatc tcgcgattgg tcgtttccag acgtcgcgac cgcgccgcct
     4741 ctccgtcaag cgcagcggcc agtcgcgtgc ggtcaatgcc gagcgcctgc acttgcgcag
     4801 caagcccctc ttcgttgcga tccgcctgct tgcgccgctc taccgcagca tcaagcgcgt
     4861 cgagcgcgag cgccagacgc ttgacggcgg catcgattgc gctttcctcg ctcattgcct
     4921 gacccgcaca aaaacgcttg ttgcggcaga tatttagaca aagaatctta cgggctgcac
     4981 agccatgacg tcaacgcttg gcggacgatt tgcccagctg ttttcgacat ctgtgaatgg
     5041 cccgcgggga gccgggcggt cacattgact caggggagtt acatgctatc caccggccaa
     5101 aaggcccatt ccatgagcgc aaaagcctcg ccgacggcag ctgcggcgcc ccgagagcgt
     5161 cgcgagcaac cggtcgctgg ttccgccatt gcccattcgg gcatggcgaa cgcgattcgc
     5221 gcgctggcaa tggatgccgt cgaacaggca aaatcggggc acccgggcat gcccatgggc
     5281 atggccgacg cggcgacgat tctctttacg cgcttcctga aattcgaccc agctgatccg
     5341 gactggtccg accgcgaccg cttcgtgctt tcggccggtc acgggtcgat gctcatatac
     5401 gcgctgctct atctcaccgg ctatgcagcg gtgacgatcg atcagatcaa gcggtttcgt
     5461 cagctcggct cgaacacggc cggccacccc gaacacggac atacgccggg cgtcgagacg
     5521 accaccgggc cgctcggaca agggcttgcg aacgcggtcg gcatggcgat cgccgagaag
     5581 cacctcgcgg cagtattcgg cgacggcatc gtcaatcaca gaacctatgt gatcgcgtcc
     5641 gacggcgacc tgatggaagg catcagccag gaagcgatcg cgctcgccgg gcatctcaag
     5701 ctcgacaagc tgatcgtgct gttcgacgac aacggcattt cgatcgatgg cgcattgtcc
     5761 ctcaccgaca gtgttgatca gctcaagcgg ttcgaggctg cgggatggaa cgcgtcgcgc
     5821 attgacggtc acgacccggc ggcgatcgcg tcggcccttg agaaggcgca gcgatccgac
     5881 aagccggtct tgatcgcctg tcgtaccaag attggtttcg gcgcgcccac caaggagggc
     5941 tccgaaaaat cgcacgggtc gccgctcggc gcggacgaaa tttcgggagc gcgcaagacg
     6001 cttggctgga cgtcggcgcc gttcgaggtg ccggccgaca ttctcaaagc gtggcgcaca
     6061 gcgggcgcgc gctcgcaagc tgtgcacgcg gactggaaaa agcggctggc ggcactcgag
     6121 agcggcaaac gtgccgagtt cgatcgccgc atgagcggca aaattccgca acaggcgctc
     6181 ggcgatgcca tccgcgccgt caaggaaaag cttgcagccc aaccgaagga catcgcaacg
     6241 cggaccgcct gcgagttcgc actcgaaagc ctgatcccgg cgttgccgga aatgatcggc
     6301 ggctccgccg atctcaccgg ctccaataac acgcgcacca agagcatgaa ggcgatcacc
     6361 gccgctgatt ttgcgggccg ctttattcac tacggcgtcc gcgagcatgg catggcggcg
     6421 gcgatgaacg gcatggcgtt gcatggcggc atcattccct attccggcac gttcctggtg
     6481 ttttccgact actgccgccc ggcaatccgg ctcgcggcgc tgatgggcaa gcgcgttatc
     6541 catgtgatga cgcacgattc gatcggcctg ggcgaagacg gaccgacgca tcagccggtc
     6601 gagcatctcg ctgcgctgcg cgcgatcccg aacttgctgg tgttccgccc ctgcgacgcg
     6661 atggaaaccg ccgagtgctg gcaactcgcg cttgcgtctg aaaaccgtcc gagcattctc
     6721 gcgctgacac ggcaaaacct gccgccgttg cgcaaccggt tcgacgccga aaatatttgc
     6781 gcgcgcggcg cctatgaaat cgccggaccg gacggcaagg ctgacgtgtc gatcttcgcg
     6841 acgggttccg aggtctcggt cgccgtcgac gctgccaagc ttttggccga gcgtggcatt
     6901 gccgcccgtg tggtggcgat gccctgcttc gagctgttcc tcgagcaaag cgaagactac
     6961 cgccgcgcca tcatcggcag cgcacgcatc aggattggcg tagaggcggc cgtgcgccag
     7021 ggctgggacg ccgtcattgg ttccgatggc ctcttcgtcg gtatgaactc ttttggcgcc
     7081 agcgcgcctt acaaggaact gtacaaaaag tttggaatta ccgcagaagc ggtcgtcgct
     7141 gcgacacttt ccaaacttgg gaagatgtga ccagacacta tatatcgcga taagaccgac
     7201 ttaagccggg agtatttcat gacagttcgt gttggcatta acgggtttgg ccgcatcggc
     7261 cgcaatatcc tgcgcgccat tgccgaagcc ggccgcaaag acattgaagt cgtcgccgtc
     7321 aacgatctcg gtcccgttga aacgaacgcg cacctgttgc gctatgacag cgtgcacggc
     7381 cgctttcccg gcaaagtgac cgtcaagggc gacatgatca gcgtcggcaa cggctccatc
     7441 aaggtcacag ccgagcgcga tccggcgaag ctgccgtgga aggatctcgg cgtcgatatt
     7501 gcgatggaat gcaccggcat tttcaccgcc aaagaaaagg cttcggctca tctcaccgct
     7561 ggggccaagc gcgttctggt gtcggcaccg gccgacggcg ccgatttcac ggtcgtattc
     7621 ggggtcaacc acgacaagct caccagggat cacaaggtcg tctcgaacgg ttcctgcacg
     7681 accaactgcc tggcgcccgt cgccaaggtt ctccacgacg ccgtcggaat cgaaagtgga
     7741 ttcatgacca cgatccacgc ctatacgggc gaccagccga cgctcgacac gctgcacaag
     7801 gatctctacc gcgcgcgcgc ggcggcaatg tcgatgatcc cgacatcgac cggcgccgcc
     7861 aaggcgatcg gtcttgtcct gcccgagctc aagggcaagc tcgacggcgt ctcgataaga
     7921 gtgccgacgc cgaacgtatc cgtcatcgac ctcaagatcg ttgccaggaa gccgacgaca
     7981 aaggaagaga tcaacgacgc cgtcaagcgc gcggcagaac agcagctgaa aggtattctc
     8041 agctacacca acgatcccaa cgtctccatc gacttcaacc acgaccctca ctcgtccacg
     8101 ttccacatgg accagaccaa agtcatcaac gggacgctgg tgcgtgtgat gtcctggtac
     8161 gacaacgaat ggggcttctc caatcgcatg gcggacacag ccgtcgcgat gggcaaatta
     8221 ctttagtgag gcattgaacc cgtcatgccc ggccttgtgc cgggcatcca cgtcttacag
     8281 ctgttcgttt ttacaaagac gtggatggcc gggacaagcc cggccatgac aaatgagtgc
     8341 tccaatggag aaatttcgta cgctcgaccg cgtcgacgta aaaggcaaac ggattctgct
     8401 tcgcgtcgat ctgaacgtgc cggtcgagaa cggcgtcgtg acggatacga cacgcatcga
     8461 gcgcgcggcg ccggcgatca ccgaacttgc cgacaaaggt ggcaaagtca tcctgctctc
     8521 gcacttcggg cgtcctaagg gccgcgaccc caagctgtct ctcaaaccgg tcgctgccga
     8581 ggtcgcacgt atcatcaagc gaccggtcag gttcgccgac gactgcatcg gcgaagcagc
     8641 cgggcgcgcg gtcggtgcga tgaagccggg tgacatcgtc tgtcttgaaa acacccgctt
     8701 tcatccaggc gaggaaaaaa acgatccggc ctttgttgcc gagcttgcaa aactcggcga
     8761 catctgggtg aacgatgcat tctcggccgc acatcgtgcg catgcctcga ccgaaggcct
     8821 tggtcacaag cttccggcct atgctggacg gacgatgcag gccgaactcg aggcgctttc
     8881 gggagcgctg gagcatcctg agcgcccact ggcggcaatt gtaggtggcg ccaagatttc
     8941 gaccaagctc gacctgctcg gcaacctgct ggacaaggtg gataccttga tcattggggg
     9001 cgccatggcc aatacgtttc tgctcgcgca gggtaaaacg gtcggcaaat cgctggtgga
     9061 agcagacctc gtcggcaccg caagggatat catggcgaag gccacgacgg ccaaacgcga
     9121 aatcgtgctg ccggtcgatg ccgtcgtcgc gcagaagttc gaagcccaag cgccatcccg
     9181 cgttgtcgat gtcgacaatg tcgatgcggc ggatatgatt cttgatattg gtccacgcag
     9241 tatcgagcag gttgtttcgg tactcgcgcg agcgaagacg ctggtttgga atggcccgtt
     9301 cggcgccttc gagatggaac cgttcgacaa cggcaccgtc gaagttgcgg aagcggcagc
     9361 ggaactgagc gaggccggca agcttgtctc ggtggcgggc ggcggagata ccgtcgcggc
     9421 actcaatgtc gcaggcgtga ccgaccgcct tacctacgtc tcaaccgcgg gcggcgcctt
     9481 cctggagtgg ctggaaggca aggccttgcc cggagtcgag gtgttgcggg taaaatgacg
     9541 ctgggaccgc cgtccgagga atctgaaggg atgcgatgga gaaagccatg aatctcgatg
     9601 aactgaacaa agtcgctcag gccatggtgg cgtccggcaa gggcatcctc gccgcggacg
     9661 aatcctccgg cacaatcaag aagcgattcg atgcgatcgg cgttgaatcg accgaggatg
     9721 cgcgtcgcga ctatcgcgag atgctcttcc gctcggccga gggcatgaag cacatttccg
     9781 gcgtcatcct ctacgacgag acgatctggc agaaggccaa ggacggaacg ccgctggtca
     9841 acttgatcag gaaggcgggc tctatccccg gcatcaaagt cgatgaagga acaaagccat
     9901 tgcccgagtg cccgggcgaa cttgtgaccg tcggcctcga caggctcgct gaccgcctcc
     9961 cgaaatatta tgaacagggc gcgcgcttcg caaaatggcg cggcgtgatt gacattggcc
    10021 ctgggattcc cacctggaca tgcgtgctcg cgaacgccca tgcactggcg agatacgcgg
    10081 cactttgcca ggccgcgcaa atcgtgccga tcgtcgagcc ggaagtgctg atggacggcg
    10141 atcacgacat cgaccgctgt tacgaggtca ccgagttcgt gctcaaggaa accttccagc
    10201 agctctatta tcagaaggtc gcgctcgaag gcatcgtgct gaagcccaac atggcggtcg
    10261 ccggcaagaa gagcgcgaag aaggcgggcg tgcaggaggt cgccgagaag accgtaaggc
    10321 ttctcaagaa ttgcgtgccc ggcgcggtgc cgggcatcgc gttcctgtcc ggcggccagt
    10381 ccgacgagga cgcgaccgcg cacctcgacg cgatgaaccg gcgcggcccg catccgtggc
    10441 agctctcgtt ctcgtacgcg cgcgcgatcc agaacgtcgc gatggagcgg tggg
//
LOCUS       DASSRL010000087         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1734347, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000087 DASSRL010000000
VERSION     DASSRL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734347"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFL49_01835"
     CDS             <1..467
                     /locus_tag="VFL49_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7910747.1"
                     /translation="TVANFNAGQGPNWLNTITTLLGHGRPLFLVLYIALIVFFAFFYT
                     AVVFNPTETADNLKKHGGFIPGIRPGERTAEYIDYVLSRITVIGALYLAIVCLVPEIL
                     ISYAAVPFYFGGTSLLIVVSVTMDTVAQIQGYLLAHQYEGLIKRSKLRGRTR"
     gene            464..1234
                     /locus_tag="VFL49_01840"
     CDS             464..1234
                     /locus_tag="VFL49_01840"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7910748.1"
                     /translation="MRLILLGPPGAGKGTQAQRLIAKHGIVQLSTGDMLRAAAAAGTP
                     VGLRVKGIMDRGELVPDDVVIAIIAERIDQPDARRGFVLDGFPRTVAQAEALDRLLAE
                     RGLKLEAVIELKVDEGILLQRIEKRIAEMTAREEKVRADDNPEVLKGRLAAYRTQTAP
                     LVGYYAGKGLLRSVDGMEPIEEVAAGIDRHLALAGAPRRKAAARSRRRKPKPSKAAKR
                     APRKGGARRKRPRAAARRGKKRPGQRKNRIRRRLTRAR"
     gene            1387..1770
                     /gene="rpsM"
                     /locus_tag="VFL49_01845"
     CDS             1387..1770
                     /gene="rpsM"
                     /locus_tag="VFL49_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET7910749.1"
                     /translation="MARIAGVNLPTNKRVVIALQYIHGIGSKTAQDIMGKVNLPPERR
                     VSQLTDAEVLQIREIIDRDYMVEGDLRREVQLNIKRLMDLGCYRGLRHRKGLPVRGQR
                     THTNARTRKGPAKTIAGKKKTPGPT"
     gene            1825..2214
                     /gene="rpsK"
                     /locus_tag="VFL49_01850"
     CDS             1825..2214
                     /gene="rpsK"
                     /locus_tag="VFL49_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7910750.1"
                     /translation="MGKEATRVRRRERKNIASGVAHVAASFNNTMITITDAQGNTIAW
                     SSAGTMGFKGSRKSTPYAAQVAAEDAAKKAQEHGMRTLEVEVSGPGSGRESALRSLQA
                     AGFTITSIRDVTTIPHNGCRPRKRRRV"
BASE COUNT          485 a          747 c          784 g          400 t
ORIGIN      
        1 cgacggtcgc caacttcaat gcggggcaag gtccgaactg gctcaacacg attaccaccc
       61 tgcttggcca tggacggccg ttgttcctcg tcctttacat cgcgctgatc gtcttctttg
      121 cgttcttcta tacggccgta gtattcaacc cgaccgaaac ggcggacaat ctgaaaaagc
      181 acggcgggtt tatccccgga atccgtcccg gcgaacggac ggcagaatac attgactacg
      241 tcctctcgcg catcaccgtc attggcgcgc tttatctggc aattgtctgc ctcgttccgg
      301 aaatcctgat ttcatacgcc gccgtgccgt tctatttcgg cggcacatcg ctgctgatcg
      361 tcgtcagcgt gaccatggac acggtcgcgc agatccaggg ctatctgctt gcgcaccaat
      421 atgaaggcct gatcaagcgc tccaagctgc gagggcgcac gcgatgaggt tgatcttgct
      481 cggcccgccg ggggcgggga agggcacgca agcgcagcgc ctgatcgcca agcacgggat
      541 cgtccagttg tcgaccggcg acatgctgcg cgcggctgcg gccgccggaa cgcccgtcgg
      601 ccttcgggtg aagggcatca tggaccgcgg cgaactggtc cccgacgacg tggttattgc
      661 aatcattgcc gaacgcatcg accagccgga tgccagacgt ggcttcgtcc tcgacggatt
      721 tccgcgcacg gttgcccagg ctgaggccct cgatcgtctg cttgccgagc gtggcctcaa
      781 gctggaagcg gtgatcgaac tcaaggtcga tgaaggcatt cttctccagc gcatcgaaaa
      841 gcggatcgcg gaaatgacgg cccgcgagga aaaggtacgc gccgacgaca atcccgaggt
      901 gctgaaaggg cggcttgccg cctaccgcac ccagacagcg ccgctggtgg gctattacgc
      961 cggcaaaggc ttgctcagga gcgtggacgg catggagccg atcgaggagg tagccgccgg
     1021 gatcgacagg cacctggcgc tggcgggggc gcccagacgc aaggctgcgg caaggtcacg
     1081 ccggcgcaag ccgaaaccct cgaaggcggc gaaacgtgcc ccgcgcaagg gcggtgcccg
     1141 ccggaaacgg ccaagggcgg ccgccaggcg cggcaagaaa aggccgggac agcgcaaaaa
     1201 ccgtatccgg cggaggttga cgagggcgcg atgaatccct taataagctg cgcattcagg
     1261 agtttgacgc gatgccgggc cccagcaagg cggggacggg cgtcgtgtta cgctttgcgc
     1321 tatccccttc aagggacttg gaaatccggc gcgttccgcg ccgtcggtcg atataggaga
     1381 aactaggtgg ctcgtatcgc gggcgtcaat ctgcccacca acaagcgcgt agtgatcgcg
     1441 ctgcagtaca tccatggcat cgggtcgaaa acagcccagg atatcatggg caaggttaat
     1501 ctgcctccgg agcgccgggt ctcgcagctg accgatgccg aggttctcca gatccgcgaa
     1561 atcatcgacc gcgactatat ggtcgaaggc gacctgcggc gcgaggtgca actcaacatc
     1621 aagcgcctga tggatcttgg ctgctatcgc gggctgcgcc accgcaaggg tctgcctgtc
     1681 cgcggccagc gcactcacac gaatgcacgc acccgcaagg gcccggccaa aacgatcgcc
     1741 ggcaagaaga agacaccggg accgacttga taacaggatg agccctcacg ccggttacgg
     1801 cgcgcgggcg cgaaaggact aagaatgggt aaggaagcta ctcgcgtccg ccggcgcgaa
     1861 cgcaagaaca tcgcgtcagg cgtcgcgcat gtcgcggcct cgtttaacaa taccatgatc
     1921 acgatcaccg atgcgcaggg caacacgatt gcctggtcgt cggccgggac catgggcttc
     1981 aagggctcgc gcaagtcgac gccctacgcc gcgcaggtcg ccgccgaaga tgccgccaag
     2041 aaggcacagg agcacggcat gcgcaccctc gaagtcgagg tgtcgggtcc tggttctgga
     2101 cgtgagtcgg cgctgcgctc gctgcaggct gccggattca cgattacttc gatccgtgac
     2161 gtgacgacga taccgcataa tggttgccgg ccgcgcaagc ggcggcgcgt gtaattgctt
     2221 gtttctcgcg ggccctggcg ctcgcgcgcg atctaccgcc gaaattttcc ccacgcggcg
     2281 ggtggggtgc gctaggcgcg agaagaccgc gccgctgaaa aggtgaagac gtgatccaga
     2341 agaattggca ggaactgatc aggccgaaca agctccaggt ctccgccggc ggcgacgccg
     2401 cgcgggtcgc gacggt
//
LOCUS       DASSRL010000088         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1226924, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000088 DASSRL010000000
VERSION     DASSRL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226924"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..361
                     /gene="rplW"
                     /locus_tag="VFL49_01855"
     CDS             53..361
                     /gene="rplW"
                     /locus_tag="VFL49_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006640319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7910751.1"
                     /translation="MALRIENVIRRPIITEKNTWLMESSQYTFEVDPEANKIQIKEAI
                     ETTFNVKVLAVNTLNVKPKKKSRVASRRGGRISGATAGWKKAIVTLDPNDHIELFEQI
                     "
     gene            383..1222
                     /gene="rplB"
                     /locus_tag="VFL49_01860"
     CDS             383..1222
                     /gene="rplB"
                     /locus_tag="VFL49_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019297986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7910752.1"
                     /translation="MAIRKYKPTSPGRRSMSVSTFEEITKKEPEKSLIEPLKRKAGRN
                     AHGRITVRHRGGGAKRFYRIVDFKRNKTGIPARVAAIEYDPNRTARIALLFYVDGEKR
                     YMLAPLGLKVGDEVISGPQAPIRVGNALPLRNIPTGTQIHNIELRQGKGGQLVRSAGV
                     AAQLMAKSGDYAQVRMPSGEVRRIHLDCMATLGQVGNVDHENVEIGKAGRARHMGKRP
                     TVRGSVMNPRDHPHGGGEGKAPIGGQPKTPWGKPAFQRTRNNKRTDSMIVRRRPANRS
                     RSR"
     gene            1316..1597
                     /gene="rpsS"
                     /locus_tag="VFL49_01865"
     CDS             1316..1597
                     /gene="rpsS"
                     /locus_tag="VFL49_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7910753.1"
                     /translation="MSRSSKKGPYIDPKLQQKVEALNRGNERRVIRTWARACTIFPGM
                     VGHTIAIHNGRQHVPVFVTEQMVGHKLGEFAPTRTYRGHGKDADRRSRR"
     gene            1633..1971
                     /gene="rplV"
                     /locus_tag="VFL49_01870"
     CDS             1633..1971
                     /gene="rplV"
                     /locus_tag="VFL49_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7910754.1"
                     /translation="MEVRASLGRVRVSARKARLVCDAVRGKRVVDAQAQLRFMPQKTA
                     FEVERLMKSVVANAENNYDLDPQDLWIKAIYADESVTYKRFQPKARGRVGKINKRTCS
                     ITVVAEERGR"
     gene            1971..>2340
                     /gene="rpsC"
                     /locus_tag="VFL49_01875"
     CDS             1971..>2340
                     /gene="rpsC"
                     /locus_tag="VFL49_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7910755.1"
                     /translation="MGRKVNPIGFRLGIVSDWESKWYAERTYTEQLHEDLKIRKLIQR
                     ELTRASISRIEIERSANKVDIKLHTAKPGIVIGKQGANVERLRQMLEKELKKKVHLSI
                     EEIKTPETDARLIAESIAEQI"
BASE COUNT          510 a          696 c          789 g          345 t
ORIGIN      
        1 aagcaggcgc ggctggccgc tgcctccgct ggcgcggaga aggaggccta ggatggcgct
       61 gcggattgag aacgtgattc gccgtccgat catcaccgag aagaacacct ggctgatgga
      121 aagcagccag tacaccttcg aggtcgatcc ggaagcgaac aagatccaga tcaaggaagc
      181 gatcgagacg accttcaatg tgaaggtgct cgccgtgaat acgctcaatg tcaagccgaa
      241 gaagaagtca cgcgtggcga gccgccgcgg tggtcggatc agcggcgcga cagccggttg
      301 gaagaaggcg attgtgacgc tcgacccgaa cgatcacatc gagctcttcg agcagattta
      361 gcggcgggga cggagaacga cgatggcgat tcgcaagtac aagccaacct cgccgggccg
      421 ccgctccatg agcgtcagca ccttcgagga aatcacaaag aaagagccgg agaagagcct
      481 gatcgagccg ctgaagcgga aggcgggccg caacgcgcat ggccgcatca cggtgcgcca
      541 ccgtggcggc ggcgccaagc gcttctaccg gatcgtcgac ttcaagcgga acaagaccgg
      601 gattccggcc cgcgtggcgg cgatcgaata cgatcccaac cggacggccc gcatcgccct
      661 cctcttctat gtggatggcg agaagcgcta catgctggcg ccgctcggcc tgaaggtcgg
      721 tgacgaggtg atctcggggc cgcaggcgcc aatccgcgtt ggcaacgcgc tgccgctgcg
      781 caacatccca accggtacgc agatccacaa catcgagctg cggcagggca agggtggtca
      841 gctggttcgc tcggccggcg ttgccgcgca gctgatggcg aaatcgggcg attacgcgca
      901 ggtgcgcatg ccgtccggcg aagttcggcg cattcatctc gattgcatgg cgacgctcgg
      961 ccaggtcggc aatgtcgatc atgagaatgt cgagatcggc aaggccggac gcgcgcgcca
     1021 catgggcaag cggccgacgg tccgcggctc ggtgatgaac ccgcgcgacc atccgcacgg
     1081 cggtggtgag ggcaaggctc cgatcggcgg ccagccgaag acgccgtggg gcaagcccgc
     1141 cttccagcgc acgcgcaata acaagcgcac tgattcgatg atcgttcgcc gccgacccgc
     1201 gaaccgatcg cgcagccgct agcgtgccgc ggccggggcg cgaggagcgc ctccgccccg
     1261 gcagaaggcg aacagcaatc tgaccacgcc gtgatggcgc ggcagggagg aaaacgtgtc
     1321 gaggtcgtca aagaaggggc cgtacatcga ccccaagctg caacagaagg tcgaggcgct
     1381 caatcgtgga aacgagcgcc gcgtgatccg cacctgggct cgtgcctgca cgatcttccc
     1441 cgggatggtg ggacacacga tcgcgatcca caatggccgc cagcatgtgc ctgttttcgt
     1501 gacagaacag atggttggac ataaacttgg tgagtttgcg cccacccgca cctaccgggg
     1561 gcacggcaaa gacgctgatc ggcggtcgcg gcgctaggcg ccgggtctcc cgaacataga
     1621 ggagtacgag acatggaagt gcgcgccagc cttggccgcg tgcgcgtctc cgcccgaaaa
     1681 gcccgtttgg tgtgcgatgc cgtgcgtggc aagcgagtgg tggatgcgca agcgcaattg
     1741 cggttcatgc cgcagaaaac cgcgttcgag gtcgagcggc tgatgaagtc ggtcgtcgcg
     1801 aatgcggaga acaactacga tctcgatccc caggatctct ggatcaaggc gatctacgcg
     1861 gacgagagcg tgacgtacaa gcggttccag ccgaaagccc gcggtcgtgt tggcaagatc
     1921 aacaagcgga cctgcagcat cacggttgtg gccgaggagc ggggacgcta atgggacgca
     1981 aagtcaatcc gatcggcttc cgcctcggga tcgtcagcga ctgggaatcg aagtggtacg
     2041 cggagcgcac ctacacggag cagcttcacg aggatctgaa gattcgcaag ctgatccagc
     2101 gtgagctgac gcgcgcgtcg atctcgcgca tcgagatcga gcggtcggcc aacaaggtcg
     2161 atatcaagct gcataccgcc aagccgggca tcgttatcgg caagcagggc gcgaatgtcg
     2221 agcggcttcg ccagatgctt gagaaggaac tgaagaagaa ggttcatctc agcatcgagg
     2281 agatcaagac gccggagacc gacgcccgcc tgatcgccga gagcatcgcc gagcagatcg
//
LOCUS       DASSRL010000089         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1401721, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000089 DASSRL010000000
VERSION     DASSRL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401721"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFL49_01880"
     CDS             <1..235
                     /locus_tag="VFL49_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7910756.1"
                     /translation="ILKDKGIKTVIAVGTSSNGAVLYTASGAASRGMNVIIPVDGMSA
                     VDPYAEYSTVFTFMNAPLVSAKTTLTKSDMIKF"
     gene            complement(316..792)
                     /locus_tag="VFL49_01885"
     CDS             complement(316..792)
                     /locus_tag="VFL49_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910757.1"
                     /translation="MSISKVLVSAVFALGLALGSVGAGVAAEPHSHGDAAAVQLRFDN
                     GKKWQTDDALRHGMGEIRQAMAASLAPIHENTFSAAKYEALAANILKHVDYVVGNCKL
                     PEEADQQLHVVLEQILDGINEMKRSSQRQQGAIKIVNALDTYGQYFDHVDWTSLAH"
     gene            complement(912..1604)
                     /locus_tag="VFL49_01890"
     CDS             complement(912..1604)
                     /locus_tag="VFL49_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7910758.1"
                     /translation="MTINAAILRRTELFAKLDVPALEEVVRHGRTRRVPRGRIIFHRG
                     DLATDCHLLLSGRIRIGQTDGGNDVVIRYVGPGQIFGALALFHGGRYPADATAVTDCV
                     EIRWSSAAITELMQRYPRIALNALAIVGHRLDELQHRVRELTTETVDRRIAHALLRLA
                     QHAGRRVKGGIEISFPLSRKDLAAMSGTAHTTVSRTLSDWQDKGIIMSGRRRIVVCDL
                     RKLEALAESQPR"
     gene            complement(1645..>2226)
                     /locus_tag="VFL49_01895"
     CDS             complement(1645..>2226)
                     /locus_tag="VFL49_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7910759.1"
                     /translation="GTERLPLDELARVAYFRPDTLPPGFQAELMATRHYVPRAWPFAF
                     TNGIQASYLEIDTDTGFVKLLKHWCVEDCGTVINPQLVDEQIRGGVVQGIGAALFEHC
                     LYDERGQLLNGNMADYLVPMAAEMPDIECGHVVSPTADSELGAKGAGEAGTAGAPGSV
                     MNAINDALRPLGAQPLTDMPFTPDKILRALGKV"
BASE COUNT          413 a          681 c          693 g          439 t
ORIGIN      
        1 gatcctcaaa gacaaaggca tcaagaccgt gattgccgtc gggacctcat caaacggcgc
       61 ggtgctctat acggcaagcg gagcggcgtc gcgcggcatg aacgtgatta tcccggtcga
      121 cggcatgtcg gcggttgatc cttatgccga atacagcacc gtcttcacgt tcatgaatgc
      181 gccgctggtc tcggccaaga cgaccttgac aaaaagcgac atgataaagt tctgatttct
      241 acaggtgctt cacgccttac aatgcaagcg gccgcgcccc gagccggcgc agccgcctca
      301 caatcttgcg acaactcagt gggccagcga tgtccagtcc acatgatcga agtactggcc
      361 gtaggtatcc agcgcattga cgattttgat tgcgccctgc tgcctctggg aggacctctt
      421 catctcattg atgccgtcga ggatctgctc caggacgacg tgcagctgct ggtcagcctc
      481 ttcgggcagc ttgcagtttc ccacaacgta gtcgacgtgc ttcagaatgt tcgctgccag
      541 ggcctcgtat tttgccgctg agaacgtgtt ctcatggatc ggcgccagcg aagcggccat
      601 ggcttggcga atttcgccca tcccgtgccg cagggcatcg tcggtctgcc actttttgcc
      661 gttgtcgaac ctgagctgaa ctgcagcggc gtcgccgtga ctatgcggtt ctgccgcaac
      721 accagcgccg actgagccga gcgccaagcc cagcgcaaat acagccgaaa cgagcacctt
      781 actgatagac atgattcctt ctcctccgac ctgctgaggt cgtgaccggg ttgcttgccc
      841 ctacaaatgg ggacccgcgg gcccgcgaga ttgctgcgga gcaacgttcc ggacaatttt
      901 ttccttttga atcagcgtgg ttggctctcg gcgagggctt cgagcttccg cagatcgcaa
      961 acgacaattc gcctacggcc gctcatgatg attcccttgt cttgccaatc gctgagcgtg
     1021 cggctgaccg tggtgtgagc ggtgccgctc attgccgcga ggtccttacg cgacaatggg
     1081 aagctgatct cgatcccgcc cttgacccgg cggcccgcat gctgtgcgag cctcagaagg
     1141 gcgtgggcga tgcggcggtc gacggtctct gtggtcaact cgcgaacacg gtgctgcaat
     1201 tcgtcgagac ggtgaccgac aatggcaagt gcgttgagcg cgattcgtgg gtatcgctgc
     1261 atcaattcgg tgatggcggc ggaagaccac cgtatttcga cgcagtccgt gaccgccgtc
     1321 gcatcggccg ggtagcggcc accgtggaac agcgcgagcg cgccaaaaat ctggccggga
     1381 ccgacgtagc ggatcacgac gtcgttgccg ccatccgttt ggccgatccg gattcgcccc
     1441 gacagcagca ggtggcagtc cgtcgccagg tcgccgcggt gaaaaataat ccggccccgg
     1501 ggaacacgcc gggtccgccc atggcgcacg acctcctcga gcgcagggac gtcgagcttg
     1561 gcgaaaagct cggtgcgtcg aagaattgcc gcgttgatcg tcattgttgg cccggggcaa
     1621 agactgcgcc ggagcgatct tacttcaaac ttttcccaac gcgcgcagga tcttgtcagg
     1681 cgtgaacggc atgtcggtaa gcggctgcgc tccgagtggg cgcagcgcgt cgttgatcgc
     1741 gttcatcacc gagccagggg cgccggccgt gcccgcttcg cccgcaccct tggcgccgag
     1801 ttcgctgtcg gcggtcggtg aaaccacatg tccacattcg atgtcgggca tctcggcggc
     1861 catcggcaca aggtaatcgg ccatgttgcc gttgagcagc tgcccgcgct cgtcgtagag
     1921 gcaatgctca aacaaagcgg cgccgatgcc ctgcacgaca ccgccgcgaa tctgctcgtc
     1981 gaccagctgc gggttgatga cggtgccgca gtcttcgacg caccaatgct tcagcagctt
     2041 gacgaagccg gtgtcggtat caatttcgag gtagctggcc tggatgccgt tggtgaaagc
     2101 gaacggccag gcgcgtggga catagtggcg tgtcgccatc agttcagcct ggaagccagg
     2161 cggcagggtg tccgggcgga aataggcgac ccgcgcgagc tcgtcgaggg gcaaacgctc
     2221 tgtgcc
//
LOCUS       DASSRL010000090         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_457280, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000090 DASSRL010000000
VERSION     DASSRL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457280"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFL49_01900"
     CDS             <1..408
                     /locus_tag="VFL49_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910760.1"
                     /translation="GQAINDLIAGHVKLGTLGSTPLIPHYRAGKLLLLAQSTAQRSPA
                     LPEVPTFREAGIELASDQWLGVFVPAGTPDAIVARLNTEVGKALADPALRKNLEDSAQ
                     EPVGGTAAAFAKLVNDDYAKFEKLVKELNIKVN"
     gene            complement(525..600)
                     /locus_tag="VFL49_01905"
     tRNA            complement(525..600)
                     /locus_tag="VFL49_01905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(565..567),aa:Val,seq:tac)
     gene            complement(733..1707)
                     /locus_tag="VFL49_01910"
     CDS             complement(733..1707)
                     /locus_tag="VFL49_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910761.1"
                     /translation="MKKLAVAIALTAFACIAHAQTQPQTYPSRQITLVVPFPPGGSTD
                     VAARILAEHMRSRLGQPVVIENVGGAGGSIAVGRVARAAPDGYTIDIGQWDTHVGSII
                     YKLDYDLQKDFEPIGLISNNPQLLVAKKDLPADNLKDLVAWMKANPGKINFVNQNAAA
                     NVSGVLFERLTGQKLHYIPYRGAGPAMTDLVSGQVDLLVVQGAAALPQVRAGKIKAIA
                     NLSPQRSPSIPDIPTSDEGGAPGLYMSGWFGFFAPKGTPKDIVAKLNAAMVQALAEPA
                     VRARFAELGLDVAAREQQTPEGLAVFQKAEIEKWWPIIKEAGITTQAQ"
     gene            complement(1866..>2377)
                     /locus_tag="VFL49_01915"
     CDS             complement(1866..>2377)
                     /locus_tag="VFL49_01915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7910762.1"
                     /translation="AAASYVRSRAVAFGIEPPLFDRRDIHVHVPEGATPKDGPSAGVA
                     MVTAIVSTMTGIPVRRDVAMTGEITLRGRVLPIGGLKEKLLAAARGGMKTVLIPEENA
                     KDLVEISDTIKKGLEIVPVTRMDEVLARALVRAPEPIEWDEASAKLAAETSGSEAAVD
                     EEGSGLTAH"
BASE COUNT          435 a          764 c          708 g          470 t
ORIGIN      
        1 ggacaggcga tcaacgatct tatcgccgga cacgtcaagc ttgggacact tggttccacg
       61 ccgctgatcc cgcactaccg ggccggcaaa ctgctgctcc ttgcacagag cacggcccag
      121 cgttcgccgg ctttgcccga agtcccgaca ttccgcgaag cgggcatcga gctggcctcc
      181 gatcagtggc ttggcgtgtt cgttcccgcc ggcactccgg acgcgattgt ggcgcggctt
      241 aacaccgaag tcggcaaggc cctagccgat cccgctctga gaaaaaacct ggaagattcg
      301 gctcaggagc cggtcggcgg cacagctgcc gctttcgcca aattggtgaa cgatgattac
      361 gcaaagttcg aaaagctggt gaaggagctc aacatcaagg tcaattaaac gtgtggatca
      421 tccgcgattc aaagcggtcc attgttctgc cgccgtcatt cgtgagtggc ctgagacaga
      481 ggtgacactt cgtaccggac acatgggcaa cacttttcgc cgtttggtgg gcggtgacgg
      541 actcgaaccg ccgacatcct gcgtgtaaag caggcgctct accaactgag ctaaccgccc
      601 tcgcagatcg tttatcgcca tcagtggatg cgaggcaagc gttgtaagat tggcgcctga
      661 atcagaacaa aacggcgcct tgcggcgcag tttttaaatt ctggcggtat tcagccggtg
      721 tgccgaagcc gcctactgcg cctgcgtcgt gatgccggcc tctttgataa tcggccacca
      781 cttctcgatt tccgccttct ggaaaacggc gaggccttcc ggcgtctgct gctcgcgcgc
      841 ggcgacatcc agccccagct cggcaaaacg cgcgcgcaca gccggctcgg caagcgcctg
      901 caccattgca gcgttaagtt tggcgacgat gtcttttggc gtgcctttgg gcgcgaagaa
      961 tccgaaccag ccggacatgt agagtcccgg ggcgccgcct tcgtccgatg ttggaatgtc
     1021 aggtatcgac ggcgagcgtt gcggagacag attggcaatt gccttgatct ttccggcacg
     1081 gacttgcggc agcgcggcgg cgccctgcac caccagcagg tcaacttgtc cggaaacaag
     1141 gtcagtcatc gcgggacccg cgccgcgata gggaatgtaa tgcagtttct ggccggtcag
     1201 cctctcgaac agaacgccgc tcacatttgc cgccgcattc tgattgacaa aattgatctt
     1261 gcccgggttc gccttcatcc acgccacgag gtctttcaaa ttgtccgcgg gaaggtcctt
     1321 cttggcgacc agaagctgcg ggttgttcga tatcagtccg atcggctcaa aatctttctg
     1381 caggtcataa tccagcttgt agataatgct gccgacgtgg gtgtcccact gcccgatgtc
     1441 gattgtatag ccgtcaggcg ccgcacgcgc gacgcgcccc accgcgatac tgccgccggc
     1501 gcctccgacg ttttcgatga cgaccggttg cccgagccgc gaacgcatgt gttcggccag
     1561 aatgcgcgct gcaacgtcgg tcgatccacc gggcgggaac ggaaccacca acgtaatctg
     1621 acgtgatgga taggtttgcg gttgcgtctg cgcgtgcgcg atgcacgcaa acgccgtcag
     1681 tgctatcgca actgcgagct tcttcatgtc gtcacccccc ggtgattgcg cctcatgggt
     1741 ctggcgcagc cgctcaggct atcaggctcc gcgacgcttg agaaatgctc gcggcccgcc
     1801 gcaacatccc cgaaacgaaa acggcgcccg cttgggcgcc gtttcaattc atgccggcat
     1861 taccgctagt gcgcggtcag gcccgaccct tcttcatcga ctgccgcctc cgagccggat
     1921 gtctccgctg caagcttggc acttgcctca tcccactcga tcggctcagg cgcgcgcacc
     1981 agagcgcgcg cgagcacttc gtccatgcgg gtcaccggaa cgatctccag gccctttttg
     2041 atcgtgtcgg agatttccac cagatccttg gcgttctcct ccgggatcag cacggtcttc
     2101 atgcctccgc gcgcggcggc aagcagcttc tccttcaggc cgccgatcgg cagcacgcgc
     2161 ccgcgcagcg tgatctcgcc ggtcatggcg acgtcacggc gcaccggaat gccggtcatc
     2221 gtcgaaacga tggccgtgac catggcgacg ccggccgacg gtccgtcttt cggcgtcgcg
     2281 ccttccggca cgtgcacgtg gatgtcgcgc cggtcgaaca gcggcggttc gatgccgaag
     2341 gcgaccgcac gcgagcggac gtaggacgcc gccgcgg
//
LOCUS       DASSRL010000091         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1226985, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000091 DASSRL010000000
VERSION     DASSRL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226985"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VFL49_01920"
     CDS             complement(<1..147)
                     /locus_tag="VFL49_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET7910763.1"
                     /translation="MRCPSCGSLDTQVKDSRPTEDSAVIRRRRVCLTCNFRFTTFERV
                     QLREL"
     gene            complement(161..1471)
                     /gene="glyA"
                     /locus_tag="VFL49_01925"
     CDS             complement(161..1471)
                     /gene="glyA"
                     /locus_tag="VFL49_01925"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7910764.1"
                     /translation="MSATSNPKPAGDVETLFSAPLSDVDPAIADAIGKELGRQRDEIE
                     LIASENIVSRAVLEAQGSVLTNKYAEGLPGKRYYGGCQYVDIAERLAIDRVTKLFGCK
                     FANVQPHSGASANAEVFFALLQPGDTFMGLNLAAGGHLTHGSPVNMSGKWFKPVPYGV
                     RRDDHRIDMEEVGRLAREHKPKLIIAGGSAYPRIIDFRAFRAICDDVGALLMVDMAHF
                     AGLVAAGVHPSPFPHAHVVTTTTHKTLRGPRGGVILSDDEEIAKKLNSAVFPGMQGGP
                     LMHVIAAKAVAFGEALRPAFRAYARNVVENSKALAETLKSHGLDIVSGGTDTHLMLVD
                     LRPKQLTGKVAEAALGRAHITCNKNGIPFDPEKPTVTSGIRLGTPACTTRGFGIAEFR
                     QVGDMIVEVLDVLSQKHVDEDSLVEAAVRDKVKTLIGRFPIYQD"
     gene            complement(1672..2184)
                     /locus_tag="VFL49_01930"
     CDS             complement(1672..2184)
                     /locus_tag="VFL49_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7910765.1"
                     /translation="MKAVAKTVEPAGREAQLDHIRPQYLEALTLVERLHRRLLDVIKD
                     EFDRRGRSDINSVQALLLYNIGDKELTAGELRTRGYYLGSNVSYNLKKLVEMAFLDHQ
                     RSRVDRRSVRIKLTDKGREVRDIIETLYQKHVRTVEQVGGINADEFATLNKSLHRLER
                     FWTDQILYRL"
     gene            complement(2392..>2817)
                     /locus_tag="VFL49_01935"
     CDS             complement(2392..>2817)
                     /locus_tag="VFL49_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET7910766.1"
                     /translation="VGKTIVHAGGAGNGQAAKICNNMILGISMIAVSEAFVLAEKLGL
                     DHQKLFDISSKSSGQCWSMTTYCPVPGPVPTSPANRDYQAGFTAAMMLKDLKLSQDAA
                     RATRTKTELGADAERIYSRYVASGEAARDFSGIIRFVRG"
BASE COUNT          560 a          885 c          827 g          545 t
ORIGIN      
        1 cagttcgcgc agttgcacgc gctcgaaagt cgtgaaccgg aaattgcagg tcaggcaaac
       61 gcggcgacgg cggatcacgg cggaatcctc ggtcggccgg gagtccttca cctgcgtatc
      121 gaggctgccg caactcgggc aacgcatacc gcccccgttc tcagtcttga tagatcggaa
      181 accgcccgat caaggtcttc actttgtcgc gtacggccgc ttccaccaga gaatcttcat
      241 cgacgtgctt ctgcgacaag acatcaagca cctcgactat catgtcgccg acctggcgga
      301 attcggcgat gccgaagcca cgcgtggtgc aggctggcgt gccgagccga atgccggacg
      361 tcaccgttgg cttctccgga tcgaacggaa tgccgttctt gttgcaggta atgtgcgcac
      421 ggcccagcgc cgcttccgcc accttgccgg tcaactgttt cggccgaagg tcgaccagca
      481 tcagatgggt gtcggtgccg cccgagacga tgtcgagccc gtgcgacttc agcgtttcgg
      541 caagcgcctt ggaattctca accacgttgc gggcgtaggc gcggaacgcc ggccgcagcg
      601 cttcgccaaa agcaacagcc ttcgccgcaa tcacatgcat cagcggcccg ccctgcattc
      661 ctggaaagac cgcagagttg agcttcttcg cgatctcctc gtcatcggac aagatcaccc
      721 cgccgcgcgg acctcgcagc gttttgtgcg tcgtcgtcgt caccacatgt gcatgcggaa
      781 acggcgacgg atgcacgccc gcggccacaa ggcctgcgaa atgcgccatg tccaccatca
      841 gcagggcgcc cacgtcatca caaattgccc gaaaggcacg gaagtcgatg atgcgcggat
      901 aggccgagcc gccggcgata atcagcttcg gcttgtgctc gcgggccagc cgcccaacct
      961 cctccatgtc gatccgatga tcgtcacggc ggacgccata gggcaccggc ttgaaccatt
     1021 tcccggacat attgaccggt gaaccgtgcg tgagatggcc gccggcggcg agattgagtc
     1081 ccatgaaagt gtcgcccggc tgcagcaacg cgaagaatac ctcggcgttg gcggaagcgc
     1141 ctgaatgcgg ctgcacgttc gcgaacttgc agccgaatag ctttgtgacg cggtcgatcg
     1201 ccagccgttc ggcaatatcg acgtattggc acccgccata gtagcgtttg cccggcaggc
     1261 cctcggcata tttgttggtg aggaccgatc cctgcgcttc caacaccgcg cgcgagacga
     1321 tgttctcgga tgcgatcagc tcgatctcgt cgcgctggcg cccgagctcc ttcccgatag
     1381 catcggcaat ggcggggtcg acatcgctca gcggtgccga gaacagcgtt tcgacgtcac
     1441 ctgcgggctt cggattcgac gttgccgaca tcacgatctc ctggcgccgc gagccgtcgg
     1501 cgccgaactg cgaaaagggt ccgggattgc gcacggcaat agcatatcgg catcaggccg
     1561 ccaagcattg ggctgtggaa cgcgtgcggc cgcaacatct agggctgcgc ccccacaaga
     1621 aaaaccccgg ccgcaaggcg gccggggcaa atccaatgct ccttaatcgg actagagccg
     1681 ataaaggatc tggtcggtcc agaagcgctc aagccggtgc agcgacttgt tgagcgtggc
     1741 gaactcgtcg gcattgatgc cgccaacctg ctcaaccgtg cgcacgtgct tctggtacag
     1801 cgtctcgatg atgtcgcgca cctcgcggcc cttgtcagtg agcttgatgc gcaccgagcg
     1861 ccgatcgacc cgcgaacgct ggtgatcgag gaacgccatc tcgacgagct tcttcaggtt
     1921 gtaggaaacg ttcgagccga gatagtagcc gcgcgtgcgc agctcgccgg ctgtcagctc
     1981 cttgtccccg atgttgtaga gaagcagcgc ctgcaccgaa ttgatatcgg aacggccgcg
     2041 cctatcgaat tcatccttga tcacgtcgag caagcggcga tgcagccgtt cgactagcgt
     2101 caaggcctcc aagtactgtg gccgaatgtg atcgagctgc gcctctcgcc cggcgggctc
     2161 gaccgtcttt gcgacggctt tcatgttcaa cgccccttgc gttttatagc tacgtttccg
     2221 gttacgaggc cctgtcttcc ccgctccgga tgaggcgcaa cttagagggg tcagtctaaa
     2281 atcggcttaa ataacaccat aaagattagg tttatttgca acggtgaacg ccctgttgac
     2341 cttacaattc taggaacgcg agcaaggctt cgttcacgct aaaaaccggc atcacccgcg
     2401 gacaaaacga atgatgccgg aaaagtcgcg cgccgcctcc ccgctcgcca cgtatcgcga
     2461 atagatgcgc tcggcatccg cgccaagttc cgttttggtt cgcgtcgctc gtgccgcatc
     2521 ttgggacagt ttgagatcct tcagcatcat ggcggcggtg aagccggcct ggtagtcgcg
     2581 gttggccggc gatgtcggaa ccggtcccgg aacaggacaa taagttgtca tggaccagca
     2641 ttgccccgag gactttgaag aaatatcgaa caacttctgg tgatcgagcc caagcttctc
     2701 ggcgagaacg aaagcttccg aaacggcgat catcgatatg ccgaggatca tgttgttgca
     2761 gatcttcgcc gcctgcccgt tgccggcacc acccgcatgc acaatcgtct tgcccac
//
LOCUS       DASSRL010000092         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1437130, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000092 DASSRL010000000
VERSION     DASSRL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437130"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..147
                     /locus_tag="VFL49_01940"
                     /pseudo
     CDS             1..147
                     /locus_tag="VFL49_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(185..1027)
                     /locus_tag="VFL49_01945"
     CDS             complement(185..1027)
                     /locus_tag="VFL49_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7910767.1"
                     /translation="MKLVRYRATGGEKPGLILDNEIFDLAGSFATLNPRAPTLDDIEA
                     IAAVPAKNLTKAPKDAVLGPPLRGIGKIIGVGLNYRDHAEETGLPLPKEPTLFLKATS
                     ALCGPTDDLVMPREAKSVDWEIELGVVIGKPGVYIAEREALDYVLGYCVGIDFSERDF
                     QFNRGGQGFKGKSADTFAPLGPFLATRDEIADPQKLSMRLTVNGEAKQSGSTRDMIFS
                     VAELVSYISRFMSLQPGDVILTGTPAGVGLGQKPPVYLKPDDVVTASIESLGEQTHKV
                     MAGA"
     gene            1110..1994
                     /locus_tag="VFL49_01950"
     CDS             1110..1994
                     /locus_tag="VFL49_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7910768.1"
                     /translation="MHPRERLPYSPIEGRPPLKLPKGVRLVVWPVLALEEWDMGRPMA
                     RMVISPPQGQPMLPDHPNWSWHEYGMRVGFWRLRKVMQKLGINPTVTLNAKVCETYPS
                     VVKACIDSGWELNAHSYDQVPMHKLDDERAVIDKSMDIIEKFSGKRPRGWFGPGLTQT
                     FETLDYLSEAGVEYIGDWVLDDEPVTLETRHKPIVALPYNFEIHDIVMMALQHHPSDE
                     WHGRALDHFDVLYAESVERLKFMALACHPYLSGVPHRIGHVERTFAEILSHDGVVAWD
                     GSKILDWYLTEGGLRVAA"
     gene            1995..>2197
                     /locus_tag="VFL49_01955"
     CDS             1995..>2197
                     /locus_tag="VFL49_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910769.1"
                     /translation="MAESYRSCFVPHDLAGPLKGAASGPLAGLSAVVKDMYDIAGERT
                     GCGSPEWLAARPPATRNCTPVQK"
BASE COUNT          415 a          683 c          684 g          415 t
ORIGIN      
        1 gagcgccata tcgactacat ccattttaat cccgtcaaac acggcctcgc gccccgcgtt
       61 cgcgattggc cgtatagtag cttccatcaa tacgtgacac gtggtgattt gccggcagat
      121 tggggcggcg atatgcgtga catgtctgat gatgacacat tcggcggatt caatcggcac
      181 cgttttacgc gcccgccatt accttgtgcg tctgctcgcc caggctttcg atcgaagccg
      241 tgacaacatc gtcgggcttt aaataaaccg gcggcttctg gccaagaccg acgccggccg
      301 gcgtgccggt aaggatcacg tcgcccggct gcaggctcat gaaccggctg atgtaggaca
      361 cgagttcggc aacggaaaag atcatgtcgc gggtcgagcc gctttgcttc gcctcgccgt
      421 tgaccgtcag ccgcatcgac agtttctgcg gatcggcgat ctcgtcgcgc gtcgcgagga
      481 atggaccgag cggcgcgaaa gtatcggcgc tcttgccctt gaagccctgc ccgccgcggt
      541 tgaactggaa gtcgcgctcg gaaaagtcga tgccgacgca atagccgagc acgtagtcga
      601 gcgcctcgcg ctctgcgatg tagacgcccg gcttgcctat caccacgcca agctcgattt
      661 cccagtcgac actttttgcc tcgcgcggca tgacgagatc gtcggtcggc ccacacagcg
      721 cactggtcgc tttcaggaat aatgtcggct ccttcggcag cggcaggccg gtttcctccg
      781 cgtgatcgcg gtagttcaga ccgacgccga taattttacc gatgccgcgc agcggcgggc
      841 cgagcacggc gtccttcggc gctttcgtca aattcttcgc cggtacggcg gcgatggcct
      901 cgatgtcatc cagcgtcggc gcgcgcggat tgagcgtggc gaagctgccc gccaggtcga
      961 aaatctcatt atccagaatg aggcctggct tctcgccgcc agtggcacgg taccggacga
     1021 gcttcatgcc ctgccctgta caacattcag agcacctgca aaactggcga aatacgggcc
     1081 gcgcagcgta acaaagacgg agctcgatca tgcatccacg cgaacgactg ccctactcgc
     1141 cgatcgaggg gcgcccgccc ctcaagctgc cgaaaggcgt gcggctggtg gtctggccgg
     1201 tgctggcgct cgaggagtgg gacatgggcc ggccgatggc gcgcatggtg atttcgccgc
     1261 cgcagggcca gccgatgctt cccgatcatc cgaactggag ctggcatgaa tacggcatgc
     1321 gcgtcggctt ctggcgtctg cgcaaggtga tgcagaagct cggcatcaat ccgacggtca
     1381 cgctcaacgc aaaagtctgc gaaacctatc cgagcgtcgt gaaggcctgc atcgacagcg
     1441 gctgggagct caatgcccac agctacgatc aggtgccgat gcacaagctc gatgacgagc
     1501 gggcggtgat cgacaaatcg atggacatca tcgagaaatt ctccggcaag cggccgcgcg
     1561 gatggttcgg acccggcctg acgcagacct tcgagacgct cgattacctg tcggaagccg
     1621 gcgtcgaata tatcggcgac tgggttctcg acgacgagcc ggtaacgctc gaaacgcgtc
     1681 acaagccgat cgtcgcactg ccttacaatt tcgagatcca cgacatcgtc atgatggcgc
     1741 tgcaacatca cccgtcggac gagtggcacg gtcgcgcgct cgatcatttc gatgtgcttt
     1801 acgcggagtc ggtcgagcgc ctgaaattca tggcgctcgc ctgccatccg tatctgtcgg
     1861 gcgtgccgca tcgcatcggc catgtcgagc gcaccttcgc cgagatcctg tcgcacgacg
     1921 gcgtcgtggc ctgggacggc agcaagattc tggactggta cctgaccgag ggcggattgc
     1981 gggtcgctgc gtaaatggcg gaatcatatc gcagctgctt tgtgccgcat gatcttgccg
     2041 ggccgctcaa gggagcggcg tcggggccgc tcgccggatt gagcgccgtg gtcaaggaca
     2101 tgtacgacat cgccggtgag cgcaccggct gcggcagccc ggaatggctc gcggcgcgtc
     2161 cgcccgccac acgcaattgt acaccggtgc agaagat
//
LOCUS       DASSRL010000093         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_964636, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000093 DASSRL010000000
VERSION     DASSRL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964636"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFL49_01960"
     CDS             <1..459
                     /locus_tag="VFL49_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7910770.1"
                     /translation="KDIITIEHMRAMKDRAIVCNIGHFDNEIQVAGLKNFKWNNIKPQ
                     VDEIEFPSGKRIILLSEGRLVNLGNAMGHPSFVMSSSFTNQTLAQIELWTNPKAYEKK
                     VYTLPKKLDEKVARLHLEKIGVKLTKLRDDQASYIGVPQDGPFKSDHYRY"
     gene            complement(434..1735)
                     /locus_tag="VFL49_01965"
     CDS             complement(434..1735)
                     /locus_tag="VFL49_01965"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7910771.1"
                     /translation="MIQQPHIVIIGAGFAGLACAKALGNAPARVTVIDRTNYHLFVPL
                     LYQVATAALSPADIARPIRRILGRYKNIDVLLANVTGIETATRVVKTDAGTVGPYDRL
                     VIATGSLYSYFGHPQWMAFAKGPRSLEDARAIRTQLLTAFEHADSAANPAERDRLMTV
                     VVVGGGPTGVEMAGSVAELARHALARDFRHIDPRRARILLIEAAPRLLASFPQSLADY
                     AKKELTRLGVTVMLGHPVENVTAEGASVGGQMIAAGTLIWGAGIEAAPGVKTLGVPLD
                     RVGRVAVGPDLSVPGLAGVYVLGDSALALDEHGQPFPALAQVAAQQGDYVGRALAAQA
                     RGKPLPKPFRFHNRGNTAIIGRNAAVFDFGRWHLKGRLAWFLWAFVHIYLLVGFENRL
                     QVVAHWLWSYLTYERGARLIMPDAESVERNQPFVSSGSGRS"
     gene            1898..>2416
                     /locus_tag="VFL49_01970"
     CDS             1898..>2416
                     /locus_tag="VFL49_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7910772.1"
                     /translation="MTASPIQNIRNFSIVAHIDHGKSTLADRLIQLTGGLEEREMKEQ
                     VLDSMDIERERGITIKAQTVRLKYKARDGNDYLLNLIDTPGHVDFAYEVNRSLAACEG
                     SLLVVDASQGVEAQTLANVYQAIDNGHEIVPVLNKVDLPAAEPAKVKQQIEDVIGLDA
                     SNAIEISAKTGLN"
BASE COUNT          500 a          770 c          728 g          418 t
ORIGIN      
        1 aaggacatca tcaccatcga gcacatgcgc gcgatgaagg atcgcgccat cgtctgcaac
       61 atcggtcact tcgacaacga gatccaggtc gcgggcctga aaaatttcaa gtggaacaat
      121 atcaagccgc aggtcgacga gatcgagttt ccgtcgggca agcgcatcat tttgctgtct
      181 gaggggcgcc tggtgaatct cggcaatgcg atgggtcatc cgagcttcgt catgtcgtcg
      241 tccttcacca accagacgct ggcgcagatc gagctgtgga ccaacccgaa ggcgtatgaa
      301 aaaaaggtct acaccctgcc gaaaaaactc gatgagaagg tcgcacgcct gcatctggaa
      361 aagatcggcg tgaagctcac caagttgcgc gacgaccagg cgtcctacat tggcgtgccg
      421 caggacggcc cgttcaagag cgaccactac cgctactgac gaacggctgg ttgcgctcca
      481 cgctttcagc gtccggcatg atcagccggg cgccgcgttc gtatgtgaga tagctccaca
      541 gccagtgcgc caccacctgc agccggttct cgaaaccgac gagcagatag atgtgcacga
      601 acgcccacag gaaccaggcc agccggccct tgagatgcca gcggccgaaa tcgaacacag
      661 ccgcattgcg gccgatgatc gcagtgttgc cgcggttgtg aaagcggaac ggtttcggca
      721 gcggcttgcc gcgcgcctgg gcggccagcg cacgtccgac ataatccccc tgctgtgcgg
      781 caacctgcgc caaggccgga aatggctgcc catgctcgtc gagcgccagg gcgctgtcgc
      841 ccagcacata gacgcccgct aggcccggca ccgacagatc aggcccaacc gcaacgcggc
      901 cgacgcggtc gagcggcacg ccgagcgtct tgacgcctgg cgcggcttcg atgccggcgc
      961 cccagatcag cgtaccagcc gcgatcatct gaccgcccac gctcgcgcct tcggccgtga
     1021 cgttctcgac cggatggccc agcatgacgg tgacaccgag cctggtcagt tccttttttg
     1081 cgtagtcggc gagcgattgc ggaaagctcg cgagcagccg cggcgcagct tcgatcagca
     1141 gaatgcgtgc gcggcgcgga tcgatgtgcc ggaaatcgcg tgcgagcgcg tgccgcgcca
     1201 gctcggcgac cgacccagcc atttcgaccc cggtcggccc gccgccgacc acaaccaccg
     1261 tcatcagccg gtcacgttcc gccggattgg cagcgctgtc ggcgtgctcg aacgccgtaa
     1321 gcagctgcgt gcggatcgcg cgcgcgtcct cgagactgcg cgggcccttg gcaaaggcca
     1381 tccattgcgg atggccgaaa taggaataaa gcgagccggt tgcaatcacc agtctgtcgt
     1441 acgggccgac agtaccggcg tcggtcttta ccacgcgcgt cgccgtctcg attccggtca
     1501 cgttggcgag cagcacatca atattcttgt aacggcccag gatgcggcgg atcggccgcg
     1561 ctatgtcggc cggggacaaa gccgccgtcg ccacctgata aagcagcgga acaaacagat
     1621 gatagttggt gcggtcgatc acggtgacgc gcgccggcgc gttgcctaac gccttggcgc
     1681 aagccaaccc tgcaaagccc gcaccgatga tgacgatatg cggctgttgg atcacgactt
     1741 tgccctgacg ctcaggctac cctgctggct tcccgttgaa acacacttaa ctgcgcaagc
     1801 gggaatacgg tttcctggca aattgggtca ccgaggcagt aacggattgc actttgactt
     1861 cttgccgcag tgcaccgcca gtgttatctg ccgcgccatg acggcatctc cgatccagaa
     1921 tatccgcaac ttttccatcg ttgcgcacat cgaccatggc aagtccaccc ttgccgaccg
     1981 gctgattcag ctgaccgggg gcctggaaga gcgcgagatg aaggagcagg tgctcgattc
     2041 catggatatc gagcgcgagc gcggcattac catcaaggcg cagaccgttc gccttaaata
     2101 caaggcgcgc gacggcaatg actacctgtt gaacctgatc gacacgcccg ggcatgtcga
     2161 ctttgcctac gaggtcaacc ggtcgcttgc cgcttgcgaa ggctctcttc tcgtcgtcga
     2221 tgcgagccag ggcgtcgaag cgcagacact ggcgaacgtc taccaggcga tcgacaacgg
     2281 tcacgagatt gtgcccgtac tcaacaaagt cgacctgccg gcggccgagc cggccaaggt
     2341 caagcagcag atcgaggacg tgatcggtct tgatgcgtca aatgccatcg agatttctgc
     2401 gaagacgggc ctcaat
//
LOCUS       DASSRL010000094         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1454641, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000094 DASSRL010000000
VERSION     DASSRL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454641"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1304
                     /gene="pcaF"
                     /locus_tag="VFL49_01975"
     CDS             93..1304
                     /gene="pcaF"
                     /locus_tag="VFL49_01975"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HET7910773.1"
                     /translation="MARDVFICDAVRTPIGRYGGALAKMRTDDLAAVPIKALLARNPK
                     VDWGKLEEVYYGCANQAGEDNRNVARMALLLAGLPDSVPGVTLNRLCASGLEAVGTAA
                     RAIRAGEMDFAIAGGVESMTRAPFVQGKAQEAFSRTAEIYDTTIGWRFINPLMKQQYG
                     IDSMPETGENVAEEFQVTRADQDAFALRSQQRAGKAIASGYFSKEIVAVEVPGGKAGP
                     VKVDKDEHPRPDTTLEQLAKLKTPFRNPGTVTAGNASGVNDGAAAMIIASEAAAKAHG
                     LKPRARILGMASAAVPPRIMGIGPVPATQKLMARLQQKIGDYDLIEINEAFASQSLAC
                     LRQLGLADDAAHVNPHGGAIALGHPLGMSGARLALTAAHGLEENGGKLALATMCVGVG
                     QGVSLALERVG"
     gene            1329..2036
                     /gene="pcaH"
                     /locus_tag="VFL49_01980"
     CDS             1329..2036
                     /gene="pcaH"
                     /locus_tag="VFL49_01980"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET7910774.1"
                     /translation="MTLVFPKESLDAYPKHLSPGYRSTVKRSPSKPLIIVPHTLSELT
                     GPVYGHDSVQEGDADLTKQHKGEPLGERIIVHGHVRDEDKRAVPDTLVEIWQANACGR
                     YVHNVDQHPAPLDPNFTGAGRTITDKKGYYRFITVKPGAYPWGNHHNAWRPNHIHFSV
                     FGHSFLSRLVTQMYFPGDPLFPFDPIFNSVTDERARQRMVSTFDLENTIPDWALCFRF
                     DIVLRGREATPQDTDKH"
     gene            2081..2695
                     /gene="pcaG"
                     /locus_tag="VFL49_01985"
     CDS             2081..2695
                     /gene="pcaG"
                     /locus_tag="VFL49_01985"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET7910775.1"
                     /translation="MSEITPSQTVGPFFAYGLAPHSRCDWKPSDIYDWKETAGANLVT
                     PDTTGTKIRVEGCVLDGDGMPINDAMIEVWQADAQGRYAHPADSRARPNTKFMGFGRS
                     ATDKGGVYSFDTVKPGSVPGPNGKAQAPHIVFCIFSRGMLRQIYTRLYFSDEASNAND
                     PILTLVPAERRGTLVAHKEMRGDLPVYRFDIRVQGENETVFFDI"
     gene            2983..>4218
                     /locus_tag="VFL49_01990"
     CDS             2983..>4218
                     /locus_tag="VFL49_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910776.1"
                     /translation="MNEQILQEISEFCRARGLAESTFGRRAVNDGKLASRLRNGGRIT
                     TDTLERIRAFMAQNSDGAPARRRVLFESTPPGILPVVKPAAPPVASGMSGEDPQRNFR
                     FFDNRQKYLLFVNTCSEKWEIASRVGLELANIHPRPPAVRVFDAGVGDGTVLARVMRS
                     MHDRFPTTPFYIVGKEISLEDIRLTLHKMADRFVEHPSTVLVLTNLAYAEAPWLAVKS
                     LSAATSLVWKELAMDGGTAHSFEKQITDLEPFLAENWKARVSAKSGNPIYERPVILVI
                     YRNDHKFLLDQIVPKPGGTVADYDLVIASQPYRARASLEFKAKRVLAPLARALGPGGR
                     MIAIHSHGHDPGMEIIRKVWPNDNPFVHNRHDLLKEVKHDLGTAGRDLNFNAYSDQRS
                     LFRYDMHTLPSEISSSIGTS"
BASE COUNT          851 a         1299 c         1276 g          792 t
ORIGIN      
        1 acggtcgagg aaactccacc gcccacgaaa cgcgagctcg acgtgctgcg cgatttgcag
       61 gcgcgcacca ggcgcgcgca agaggcggcg taatggcgcg cgacgtcttt atttgtgacg
      121 cggtccgtac gccgatcggc cgttatggcg gcgcgctcgc caagatgcga accgacgacc
      181 ttgcagcggt gcccatcaag gcgctgctgg cgcgaaaccc aaaggtcgac tggggaaagc
      241 tcgaagaggt ctattacggc tgcgccaatc aggccggcga ggataatcgc aacgtcgcgc
      301 gcatggcgct tttgctcgca gggttgccgg acagcgtgcc gggcgtgacc ttgaaccggc
      361 tatgcgcgtc cggcctcgaa gcagtcggca ccgccgcccg ggctattcgc gccggcgaga
      421 tggactttgc gattgccggc ggggtcgaat caatgacgcg cgcacctttc gtgcagggaa
      481 aagcgcagga agcgttctcg cgcaccgccg agatctacga cacgacgatc ggctggcgct
      541 tcatcaatcc gttgatgaag cagcagtacg gcatcgattc catgccggaa accggcgaga
      601 acgttgcgga ggaattccag gttacccgcg ccgaccagga tgcgtttgcc ttgcgctcgc
      661 aacagcgcgc cggcaaggcg atcgcttcag gctatttttc caaggaaatc gttgcggtcg
      721 aagtgccggg cggcaaggcg ggaccggtaa aggtcgacaa ggacgagcat ccgcgtcccg
      781 acacgacact cgagcagctc gcaaaactca agacgccatt ccgcaatccg ggcaccgtga
      841 cggccggcaa tgcgtccggc gtcaatgacg gcgcggcggc catgatcatc gcgtcggagg
      901 cggcggccaa ggcgcacggc ctcaaaccgc gtgcacgaat actcggcatg gcctctgccg
      961 cagtgccgcc gcgcatcatg ggtatcggac cggtgccagc tacgcaaaag ctgatggcgc
     1021 ggctgcagca aaaaatcggc gactatgatt tgatcgaaat caatgaagct ttcgcatcac
     1081 agtcgctagc ctgtttgagg caattggggc ttgccgatga tgccgcgcac gtcaatccgc
     1141 atggcggtgc gattgcgctc ggtcacccgc tcggcatgtc gggcgcgcgt cttgcactga
     1201 cggccgcgca cggcctggaa gagaatggtg gaaaactggc gcttgcgacg atgtgtgtcg
     1261 gtgtcggtca aggcgtgtcg ctggccctgg agcgggtcgg ctaaggaaaa taaaaagggg
     1321 caacaaaaat gacactggtt tttccgaagg aaagcctgga cgcatatccc aagcatctca
     1381 gtcccggcta tcgcagcacg gtcaagcgat cgccgtcgaa gccgctgatc atcgtgccgc
     1441 acactttgtc ggagctgacc gggccggtct atggccacga ttccgtgcag gaaggcgacg
     1501 ccgatctcac caagcaacac aagggcgagc cactgggcga gcgcatcatc gtgcatggcc
     1561 acgtgcgcga tgaagacaag cgtgcggtgc ccgatacatt ggtggaaatc tggcaggcga
     1621 acgcctgcgg ccgctacgtg cacaatgtcg accagcatcc ggctccgctt gatccgaatt
     1681 tcaccggcgc ggggcggacg atcaccgaca agaagggcta ctaccgcttc atcacagtga
     1741 agccgggcgc ttacccttgg ggcaatcacc ataatgcctg gcggccgaac catattcatt
     1801 tttcggtgtt cggtcactcg ttcctgtcgc ggcttgtcac ccagatgtat tttccgggcg
     1861 atccgttgtt tccgttcgac ccgatcttca attcggtgac ggacgagagg gcgcgccagc
     1921 gcatggtttc gacattcgat ctggagaaca cgattcccga ttgggcgctc tgtttccgct
     1981 ttgatatcgt gctgcgcggg cgcgaagcca caccacaaga tacggacaag cactagcgcg
     2041 tgatcgtgtt gaacaagatc gtgcgcaaag gaaaacgtta atgtcggaaa taaccccctc
     2101 tcagaccgtc ggtccattct tcgcgtatgg gctcgcgccg cacagccgtt gcgactggaa
     2161 acccagcgat atctatgact ggaaggagac tgccggcgcc aatctggtga cgccggatac
     2221 gaccggcacg aagatccggg tcgagggttg cgtgctcgac ggtgatggca tgccgatcaa
     2281 tgatgcgatg atcgaggtct ggcaggcgga tgcacagggc cgctatgcgc atccggccga
     2341 cagcagggcg cgcccgaaca ccaaatttat gggattcggc cgctcggcga ccgacaaggg
     2401 cggcgtctac agtttcgaca ccgtcaagcc ggggtcggta cccggcccga acggaaaggc
     2461 gcaggccccg cacatcgtgt tctgcatctt ttcgcgcggc atgctgcgtc agatttatac
     2521 ccgcctctat ttttccgatg aggcgtcgaa tgcgaacgat cccatcctga cgctggttcc
     2581 ggctgagcgg cgcgggactc tcgttgccca caaggagatg cgcggcgacc tgccggtgta
     2641 ccgcttcgac atccgcgttc agggcgagaa cgagacggtt ttcttcgaca tctgacctgc
     2701 cgacgccgtt ttccagcctc aaaccactgc gctggacagt ccaactctgc ccaccatcgt
     2761 tgtcgagtat tgtaggaaaa tgcccacagt actgaccgcg tgacgaggga aataaagtcc
     2821 tcgtcgcatt tgttgctgag aatcttattg ctttgacgat gggcagttcg cccacccatc
     2881 ttgcccatct ttcccaactt tatatttata gttgacgctt gggaccgaag ttggttcgct
     2941 cggtggcaac ggccttcgag agccgatgag gaagcgccag ccatgaacga acaaattctc
     3001 caggagatca gcgaattctg ccgcgcgcgc ggactcgccg agtcgacctt cggccgccgc
     3061 gccgtcaatg atggcaagct ggccagccgc ctgcgcaatg gcgggcgcat cacgaccgac
     3121 acgctcgagc gcattcgcgc cttcatggcg cagaattccg acggcgctcc ggcgcgtcgc
     3181 cgcgttctgt tcgagtcgac accgcccggc attctgccgg tcgtgaagcc tgcggcaccg
     3241 ccggtcgctt ccggcatgtc cggcgaggat ccgcaacgca acttccgttt cttcgacaac
     3301 cggcagaaat atctgctgtt cgtgaacacc tgctcggaga aatgggaaat cgcctcacgc
     3361 gtcggactgg agcttgccaa tatccacccg cgcccgcccg cggtgcgcgt attcgatgct
     3421 ggcgtcggcg acggcaccgt gctggcgcgc gtgatgcgct cgatgcacga tcgcttcccc
     3481 accacgccgt tctatatcgt cggcaaagag atcagcctcg aggatatccg cctcacgctg
     3541 cacaagatgg ccgaccgttt cgtcgagcat ccatcgacgg tgctggtact caccaatctc
     3601 gcctacgcgg aagcgccctg gctggcagtc aaatcgctca gcgccgcgac cagcctggtg
     3661 tggaaagaac tggcgatgga cggcggcacc gcgcacagct tcgaaaagca aatcaccgac
     3721 ctcgagccgt ttcttgccga gaactggaag gcgcgggtca gcgcaaaaag cggcaaccca
     3781 atctacgagc gccccgtgat tctggtgatt taccgaaatg atcacaaatt cctgctcgat
     3841 cagatcgtgc ccaagccagg cggcactgtc gccgactacg atctcgtcat cgcgtcgcag
     3901 ccttatcggg cgcgggcgtc gctcgagttc aaggccaaac gtgtgctggc gccgctcgcg
     3961 cgcgctctcg gtccgggggg gcgcatgata gcgatccact cccatggtca cgatccgggg
     4021 atggaaatca ttcgcaaggt ctggccgaac gacaatccgt tcgttcacaa ccgccacgac
     4081 ctgctcaagg aggtcaagca cgatctcggt actgccggtc gcgatctcaa tttcaatgcc
     4141 tattctgacc agcgttcgct gtttcgctac gacatgcaca cgttaccctc tgagatctct
     4201 tcatcgatcg ggacgtcg
//
LOCUS       DASSRL010000095         4492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1524738, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000095 DASSRL010000000
VERSION     DASSRL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524738"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VFL49_01995"
     CDS             complement(<1..1622)
                     /locus_tag="VFL49_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET7910777.1"
                     /translation="MRPMNKPMATSDSIPATPLAAEVDRRRTFAIISHPDAGKTTLTE
                     KLLLFGGAINLAGQVKAKRDRRSTRSDWMAIERERGISVVTSVMTFDYDGRVFNLLDT
                     PGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRE
                     SRDPFELLDEIEKTLALDTAPMTWPIGRGRDFAGTYDLSTGEMRLVAEDGKTVERQAL
                     DLPGLAAKNPMLDARAIADELALVRDACRPFDFKSFREGHLTPVFFGSALKNFGVKDL
                     LDSLASFAPAPRAQVANVRTIDAHENAMTAFVFKIQANMDPNHRDRIAFARLCSGKLR
                     RGMKAKLTRTAKPMTLSTPQFFFAQDRALAEEAYAGDVVGIPNHGTLRIGDTLTEGED
                     IAFLGVPNFAPEILRRVKLGDPMKAKKLKEALQQMAEEGVVQVFRPLDGAPALVGVVG
                     PLQLDVLKVRLSGEYGLDVGFEQPEFQLARWISSDDGRRLEEFVQANYSSCAEDLDGD
                     KVFMARNQFILDYTQERAPGISFTDIKYVKDRA"
     gene            complement(1699..2049)
                     /locus_tag="VFL49_02000"
     CDS             complement(1699..2049)
                     /locus_tag="VFL49_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HET7910778.1"
                     /translation="MSATIYGIKNCDTMKKARAWLDKHGVDYAFHDYKSAGIDRSQLE
                     RWAKKVGWETLLNRAGTTFRKLPDKDKDGITATKAMALMLKQPSMIKRPVLEAGDGKL
                     LVGFRPEQYAAIFG"
     gene            complement(2090..2440)
                     /locus_tag="VFL49_02005"
     CDS             complement(2090..2440)
                     /locus_tag="VFL49_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910779.1"
                     /translation="MTDVSMSSVVSHDAETCAGGGHAASGAAGWLGLAATPTFGIMAL
                     WTSLFSGLPDMFCMGMRDASPLNGMALMYALMSAFHAAPWLRLISADAVRRAWPLRRT
                     HRCSGCRTKNCPVE"
     gene            complement(2433..3197)
                     /locus_tag="VFL49_02010"
     CDS             complement(2433..3197)
                     /locus_tag="VFL49_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HET7910780.1"
                     /translation="MTTHMTATRDKWLEARLDLLAAEKDPTRRSDEVAQLRQRLPWVR
                     VEKAYRFDTEEGMASLADLFKGRSQLLVYHFMFGPDYKAGCPSCSSIADGFNGIFVHL
                     ENHDVAFTAVSRAPIAKLQAFKQRMGWSFPWASSVGGDFNYDFNVSITEEQQRRGNFE
                     YNYARATHAMDAKSVPPIVTANAAMTGADPVAFVRERPGMSAFVLEDGVVYHAYSTYA
                     RGLDGLWGMYQWLDRAPKGRNETGVWWRHHDRYGHD"
     gene            3324..>4492
                     /locus_tag="VFL49_02015"
     CDS             3324..>4492
                     /locus_tag="VFL49_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7910781.1"
                     /translation="MDSLITAAARALATGNPLTALNFIALRDDAPALALRGIAMAQLG
                     DLARAKTLLRRATRAFGPRNTVARARCVVAEAEIALVSRDLGWPAKPLDSARTVLERH
                     GDRANAAHARYLSIRRLLLIGRLDQAERLIEDFDPAPLPTASRAAYQLAVGGIAIRRL
                     QIKAARAALMQAKDIAREARIPSLVAEAESASHILDTPAARLIARDKEQPLRLDDIEA
                     LLASRALIVDACRHVICQSGKVISLERRPVLFALARALAEACPGDVSRAVLLARAFRA
                     KHADESHRARLRVEIGRLRKTLREIAGIDATKDGFTLVPRRARSVAVLAPPTDEAHGT
                     VLALLADGETWSSSALALALGASQRTVQRALDDLTADGKVQTIGRGRARRWMAPPV"
BASE COUNT          803 a         1446 c         1375 g          868 t
ORIGIN      
        1 gcccggtctt tcacatattt gatgtcggta aaggagattc ccggggcgcg ctcctgggtg
       61 taatcgagga tgaattgatt gcgcgccatg aaaaccttgt caccgtcaag gtcttcggcg
      121 cagctcgaat agttggcctg cacgaactcc tccagcctcc gtccgtcgtc cgatgagatc
      181 caacgtgcga gctgaaattc gggctgttca aagcccacgt cgagaccgta ttccccggac
      241 agccgcactt tcaggacgtc gagttgcaag gggccgacga caccgaccaa tgcgggcgcg
      301 ccatcaagcg ggcggaagac ctggacgacg ccttcttccg ccatctgctg cagcgcctct
      361 ttcagctttt tggccttcat tggatcgccc agcttgacgc ggcgcagaat ttcgggtgcg
      421 aaattcggaa cgccaagaaa cgcaatgtcc tcgccttcag tgagcgtgtc gccgatccgc
      481 agcgtgccgt gatttgggat gcccaccaca tcaccggcat aggcctcttc ggcgagcgcg
      541 cggtcctgcg caaagaaaaa ctgcggcgtg gacagcgtca ttggctttgc cgtgcgcgtc
      601 agcttcgctt tcatgccgcg cctgagtttt cccgagcaaa gccgcgcgaa ggcaatgcgg
      661 tcacggtggt tcggatccat gttggcctgg atcttgaaca cgaacgccgt catcgcgttt
      721 tcgtgcgcgt cgattgtcct cacattggca acctgcgcgc gcggggccgg cgcaaaggac
      781 gccagcgaat ccagcaggtc cttgacgcca aaattcttca gcgccgagcc gaagaatacc
      841 ggggtcagat ggccctcgcg aaatgacttg aagtcgaatg gccggcacgc gtccctcacc
      901 agcgcaagct catcggcgat cgccctggca tcgagcatcg ggtttttcgc cgcaagtccc
      961 ggcaagtcga gtgcctgccg ttcgacggtt tttccatcct cggcgacaag acgcatttcg
     1021 ccggtcgaca ggtcgtaagt gccggcaaag tcgcggccac ggccgatcgg ccaggtcatt
     1081 ggcgctgtgt cgagcgccag cgttttttcg atctcgtcga gcagttcgaa cggatcgcga
     1141 ctttcccgat ccatcttgtt gatgaacgtg atgatcggaa tatcgcgaag ccggcagacc
     1201 tcgaaaagtt tgcgcgtacg cgcctcgatg cccttggcgg cgtcgatcac catcacagca
     1261 gaatcgaccg cagtgagcgt gcgataggtg tcctcagaaa agtcctcgtg acccggcgtg
     1321 tcgagcagat tgaatacgcg tccgtcgtaa tcgaaagtca tcaccgacgt gaccacggag
     1381 attccgcgct cacgttcgat cgccatccag tctgaacgcg tcgaacggcg gtcccgtttg
     1441 gctttcacct gaccagcaag gttgatagcg ccgccgaaga gcaacaactt ttccgtcagt
     1501 gtcgttttgc ccgcgtccgg atgcgaaatg atcgcgaatg tgcgccggcg gtcgacctcg
     1561 gcggcaagcg gtgtcgccgg gatcgaatcg gacgtggcca tcggcttgtt catggggcgc
     1621 atgtggcagg gaaagcaggc aggatcaagg gctggcagcg ccaaaacacc ggcaaatgcg
     1681 cagcttctgt ctcgttagtt agccgaatat tgccgcgtac tgctccggcc tgaagccgac
     1741 cagcaatttt ccgtcacctg cctcgagcac aggccgtttg atcatcgacg gctgcttcag
     1801 catcagggcc atggccttcg tcgcggtgat gccgtcttta tccttgtcgg gcaattttct
     1861 gaacgtggtc cctgcccggt tgagcagcgt ctcccagcca actttcttcg cccaacgttc
     1921 gagctgagag cgatcgatgc ctgcgctctt gtaatcgtga aaggcatagt cgacgccgtg
     1981 cttgtcgagc caggcgcgcg ccttcttcat cgtatcgcag ttcttgatgc cgtagatggt
     2041 ggcagacatg tctactccca ctcaatggtt ctaagccatg gtcacgctct tattcaacag
     2101 ggcaattttt tgttcgacag ccgctgcagc ggtgcgtccg cctaagtggc catgcccggc
     2161 ggacagcgtc ggcggagatc agtctcagcc acggcgccgc atggaaagcg ctcatcagcg
     2221 cgtacatcag cgccattccg ttcaacggcg atgcatcccg catgcccatg caaaacatgt
     2281 cgggtaggcc gctgaacaag cttgtccata gcgccatgat cccgaaggtc ggcgtggcgg
     2341 caagaccgag ccagccggct gcgccggaag cggcgtgacc gcccccggca caggtctcgg
     2401 cgtcgtgact cacaacggaa gacatcgaca cgtcagtcat ggccgtaccg gtcgtggtga
     2461 cgccaccaga cacccgtctc gttgcggccc ttgggggcgc ggtcgagcca ctgatacatg
     2521 ccccacaggc cgtccaggcc gcgcgcgtaa gtcgagtagg cgtggtacac gacgccatct
     2581 tccagcacga acgcgctcat gcccggcctc tcgcgcacaa acgcgacggg atcggcacct
     2641 gtcatggctg cgttcgccgt gacgatcggc ggaaccgatt tcgcgtccat cgcgtgagtg
     2701 gcccgagcgt agttatattc gaagttgcct cggcgctgtt gctcttcggt gatcgagacg
     2761 ttgaagtcgt agttgaagtc gccgccgacc gaagatgccc agggaaacga ccagcccatc
     2821 cgctgcttga acgcctgcag cttcgcgatc ggcgcccgcg acaccgccgt gaatgcaacg
     2881 tcgtgatttt ccagatggac aaaaatgccg ttgaacccgt cggcgatcga cgagcacgac
     2941 gggcagcccg ccttgtagtc gggcccgaac atgaagtggt agaccaggag ctgcgagcgc
     3001 cccttgaaca ggtcggcgag cgaagccatc ccctcctcgg tgtcgaaccg gtacgccttc
     3061 tcgacccgga cccagggcaa gcgctggcgg agctgcgcga cttcgtcgct gcgccgcgtc
     3121 gggtccttct cggcagcgag gagatcgagc cgcgcttcca gccacttgtc gcgtgttgcg
     3181 gtcatatgtg tcgtcatctt tctctccttt cgttgcctcg gctgtctgcc gcgcttccgc
     3241 gacggcttgc cgggcattga tcgtgagcta gatttagtgc cggccatcga atggccggga
     3301 gtgacaagtg tgtcgggatt tcaatggact cgttgatcac cgccgcggcg cgcgcactcg
     3361 caactggaaa tccgctcacc gcattgaact tcatcgcctt gcgcgacgat gcgccggcgc
     3421 tggcgcttcg cggcatcgcg atggcgcagc tcggcgatct cgctcgcgcc aagacgctgc
     3481 tgcgccgtgc aacccgcgcc ttcggtccgc ggaacactgt cgcccgcgca cgttgcgtgg
     3541 tcgccgaggc cgagatcgca ctcgtctccc gcgacctcgg ctggccggcg aaaccgctcg
     3601 attcagcacg gacggtgctt gaacggcacg gcgatcgtgc caacgccgcg catgcgcggt
     3661 atctctcgat ccggcgtctg ctcctgatcg gccgccttga tcaggccgag cgcctgatcg
     3721 aggacttcga tcccgcaccg ctgccgaccg cctcgcgtgc cgcctaccaa ctggccgtcg
     3781 gcggaatcgc gatccgacgc ctgcagatca aggccgcacg cgccgccctg atgcaggcca
     3841 aagacattgc gcgcgaagcc cgcatccctt cgcttgtcgc tgaagccgaa agcgcgtcgc
     3901 acatcctcga tacgcccgcg gcgcggctca ttgcgcgaga caaggagcag cctctgcggc
     3961 tcgacgatat tgaagcgctg ctggcgtcga gggcgctcat tgtcgacgcc tgcaggcatg
     4021 tcatctgtca atccggaaag gtaatttcgc tggaaaggcg cccggtgctg ttcgcgcttg
     4081 cccgcgcact cgccgaggcc tgtcccggcg acgtgtcgcg agctgtactg ctggcgcgcg
     4141 cgttccgcgc gaaacacgct gatgaatcac atcgcgcgcg actgcgtgtc gagatcggcc
     4201 gcctgcgcaa gacactgcgt gaaatcgccg gcatcgatgc gaccaaggac ggctttacgc
     4261 tcgtgccgcg ccgcgcgcgc agtgtcgcgg tgctcgcgcc accgacagat gaagcacatg
     4321 gaaccgtgct tgccctgctg gcggatggcg aaacatggtc gagctcggcg ctggcgctcg
     4381 cgctcggcgc cagccagcgc accgttcagc gcgcgctcga cgatttgacc gcagacggca
     4441 aggtgcagac gatcggccgt ggccgggcgc gccgctggat ggcgccgccg gt
//
LOCUS       DASSRL010000096         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_895031, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000096 DASSRL010000000
VERSION     DASSRL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895031"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFL49_02020"
     CDS             complement(<1..185)
                     /locus_tag="VFL49_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910782.1"
                     /translation="MQEIDNIGRRTCLDRAQATVLTAEEYKRMSALGLTFMHFDHTPD
                     EDFVVAAVVAVVGFALER"
     gene            201..581
                     /locus_tag="VFL49_02025"
     CDS             201..581
                     /locus_tag="VFL49_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HET7910783.1"
                     /translation="MSVNKKHDEFHTLDMNVGWETPPGYPKGIEQKILSGALDETNKH
                     GTRTRLLRFAPGVFTTTPFVHEYWEEVFLVSGDLTVGNDARGNGGESFPPNTYAVRPP
                     GAYHGPFKSNGGCILLEMHYFDPV"
     gene            complement(563..2053)
                     /locus_tag="VFL49_02030"
     CDS             complement(563..2053)
                     /locus_tag="VFL49_02030"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET7910784.1"
                     /translation="MQDVETLWADFATPIKTADDYINSLRGRRMNVYFMGERVPEPVD
                     HPVIRPSVNAMAETFRVATERPDLGTVHSDISDRQINRFLHVPVAPGELVAKHEMQRE
                     LGRRTGTCFQRCVGLDAIGTCHSVTFDIDARNGTDYHRRFLAFLKRAQAANVVIGGAM
                     TDPKGDRSKAPHQQKDIDLFLHVTRRDQNGIYVSGAKMHQTGAVNSHWLIFMPTMRMT
                     EKDRDWSVVGAVRVDAPGLTYIVGRQTNDTRIMDGGAMDAGNAQFAGQESLIVFEDVF
                     VPQEHVFMDGEWDYATSLVERFTTYHRSSYVCKTGLGDVLIGAAASAAEHNGVEAASH
                     IKDKLVEMTHLNETIYSSCMAGAHQSKPVASGAYINDEMLSNVAKHNVTRFPYEIARL
                     AQDIAGGLVVTMPSERDLQNNEIGPLIRKFLQGRDGVEVTDRMRILRLIENMTIGRNA
                     VGYLTESLHGAGSPQAQRIQIARGMKIEEKKARARRLAGVAQTGSK"
     gene            complement(2092..3636)
                     /locus_tag="VFL49_02035"
     CDS             complement(2092..3636)
                     /locus_tag="VFL49_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET7910785.1"
                     /translation="MHTDLIAPIPELLKRHAAMRGSKTAYRDSRSSVTYAELLDRTGR
                     LAGHLADNGIEANDTVAIMLPNSVQWVEACFAITRAGAIGVPISYDATESEIAYRLTD
                     ANCKAAFTTGERGDLFAKLKGQAPNLKTLVVTDRGQCSADALQYAKLLASTAKSAPRD
                     PASLYEPMYILYTSGTTGRAKGVQLTVHGMLWIAAACWAPITGLSDKDTVLSPLPLFH
                     SYALNLSVMTILATGATEYIMEKFSTNEAARLLKTGEFTYFPGVPTMFHYLLQATRAE
                     KDVRFPNLRLCASAGAIMPATLNREFEAHLGVPLLDGYGITETSTMVTMNWPSGGRTL
                     GSCGLPLPGLTVRIVDPASGKDCKPGQEGELIVRGPNVMPGYHNKPEETRNALREGWY
                     HTGDLARSDENGFLTITGRLKELIIRGGQNIAPAEIEEVVNTFEAVLDCAVVGMPHEH
                     LGEVPALFVVPRPGKTVENEALIAHCRMHLSAYKVPHSVQLIAEIPRTGSGKIIRYKL
                     KETMRA"
BASE COUNT          650 a         1164 c         1125 g          698 t
ORIGIN      
        1 cgttcgagtg cgaagcctac gaccgccacg acggcggcga ccacgaaatc ttcgtcgggc
       61 gtgtggtcga aatgcatgaa agtcaggcca agcgcggaca tccgcttgta ttcttcggcg
      121 gtcagtaccg tcgcctgggc gcggtcgagg caagtgcgcc ggccgatatt gtcaatttcc
      181 tgcacggatg gtgaaggacc atgagcgtaa acaagaaaca cgacgaattc cacaccctcg
      241 acatgaacgt cggctgggag acgccgcccg gctatccgaa aggcatcgag cagaagatcc
      301 tgtcgggcgc gcttgacgag accaacaagc acggcacgcg cacgcggctg ctgcgcttcg
      361 cacccggtgt gttcactaca acacccttcg tccacgagta ctgggaagaa gtctttctcg
      421 tgtcgggcga cctcaccgtc ggcaacgacg cgcgaggaaa cggcggtgag tcattcccac
      481 ccaacaccta tgcagtccgt ccgcctggcg cttatcatgg gccattcaag tcaaatggcg
      541 gatgcatcct tctggaaatg cactacttcg acccggtctg agcgacgccg gcaagacgcc
      601 gggcacgcgc cttcttctcc tcgatcttca tgccgcgcgc gatctggatg cgctgcgcct
      661 gcggcgagcc ggcgccgtgc aggctttcgg tcagatagcc gacggcattg cggccgatgg
      721 tcatattctc gatcagccgc aggatacgca tccggtcggt gacctcgacg ccgtcgcgcc
      781 cttgcaggaa cttgcggatc agcgggccga tctcattgtt ttgcaagtcc ctttctgacg
      841 gcatcgtgac gacaaggccg ccggcgatgt cctgcgccag ccgcgcgatc tcgtaaggaa
      901 accgcgtgac gttatgcttg gcgacgttcg agagcatctc gtcgttgata taggcacccg
      961 aagcgaccgg cttcgattga tgcgctcctg ccatgcagct cgagtagatc gtctcgttga
     1021 gatgcgtcat ctcgaccagc ttgtccttga tgtgcgaggc ggcttctacc ccattgtgct
     1081 cggcggccga cgcggcggcg ccgatcagca cgtcgccgag cccggttttg cagacataag
     1141 atgagcggtg ataggtggtg aagcgctcga caagcgaggt cgcgtagtcc cattcgccgt
     1201 ccatgaacac gtgctcctgc ggcacgaaca cgtcctcgaa cacgatcagc gattcctggc
     1261 cggcaaactg cgcgttgccg gcgtccatcg cgccgccgtc catgatgcga gtgtcgttgg
     1321 tctgccgccc gacaatgtag gtaaggcccg gggcatcgac gcggaccgca ccgacgaccg
     1381 accagtcgcg atccttttct gtcatgcgca tggtcggcat gaaaatgagc cagtgcgaat
     1441 tgaccgcgcc ggtctggtgc atcttggcgc cggagacgta gatgccgttc tggtcgcgcc
     1501 tggtgacgtg caggaaaaga tcgatgtcct tctgctgatg cggcgccttg gagcggtcgc
     1561 ctttcggatc ggtcatcgcg ccgccgatca cgacattcgc cgcctgcgca cgcttcagga
     1621 aagcaagaaa gcgcctgtgg tagtcggtgc cgttcctggc gtcgatatcg aaggtcaccg
     1681 aatgacaggt gccgatggca tcgagtccga cgcagcgctg gaagcaggtc ccggtgcgcc
     1741 gccccagctc gcgttgcatc tcgtgcttgg cgacaagctc cccaggcgcg accggcacgt
     1801 gcaggaaacg gttgatctgc cgatcactga tgtccgagtg caccgtgccg aggtccggcc
     1861 gctcggtggc gacgcggaat gtttccgcca tcgcgttgac cgacggccgg atcaccggat
     1921 gatcgaccgg ctccggcaca cgctcgccca tgaagtagac attcatgcgc cggccacgca
     1981 gcgaattgat gtagtcgtcc gcggtcttga tgggggtggc gaagtccgcc cacagcgtct
     2041 cgacgtcctg catcacgcca tctccttcaa acgcgccctg ccgccctgcc ctcacgccct
     2101 cattgtttcc ttgagcttgt aacgtatgat cttgcccgaa cccgtgcgag ggatctcggc
     2161 aatcaattgc acgctatgcg gcaccttgta cgccgacaga tgcatgcggc aatgggcgat
     2221 cagcgcctca ttctcgaccg tcttgccggg ccgcggcacc acgaacagcg ccggaacctc
     2281 gccgagatgc tcgtgcggca tgccgaccac ggcgcaatcg agcaccgcct cgaaggtgtt
     2341 caccacttct tcgatctccg ccggcgcgat gttctgcccg ccgcggatga tcagctcctt
     2401 caggcggccg gtgatggtga ggaaaccgtt ttcgtcgctc ctggcgagat cgccggtgtg
     2461 ataccagccc tcgcgcagcg cgttgcgcgt ctcctcgggc ttgttgtgat agccgggcat
     2521 cacgttggga ccgcgcacga tcagctcgcc ctcctggccc ggcttgcaat ccttgccgct
     2581 tgccgggtcc acgattcgca cggtcagccc gggcaagggc aggccgcagg agccgagcgt
     2641 acgcccgccc gacggccagt tcattgtcac cattgtcgag gtttcggtga tgccgtagcc
     2701 gtcaagcagc ggcacgccga gatgcgcctc gaattcgcgg ttcagcgtgg ccggcatgat
     2761 cgcgcccgcc gacgcacaca agcgaaggtt cgggaaccgc acgtcctttt cggcgcgcgt
     2821 cgcctgcaat aaataatgaa acatcgtcgg cacgccggga aagtaggtga actcgccggt
     2881 cttgagcaga cgcgccgctt cgttcgtaga aaatttctcc atgatgtatt cggtcgcgcc
     2941 ggtcgccagg atcgtcatca ccgacaaatt caacgcgtag gaatggaaga gcggcagcgg
     3001 cgacagcacg gtatccttgt cggacagccc ggtgatcggt gcccagcagg cggcggcgat
     3061 ccacaacatg ccgtgcacgg tgagctgcac acccttggcg cggcccgtgg tcccggacgt
     3121 gtagagaatg tacatcggct cgtacagcga ggccggatcg cgcggcgccg atttcgccgt
     3181 cgaggcgagc agtttggcgt actgcaacgc gtccgcactg cactggccgc gatcggtcac
     3241 gaccagcgtc ttgagattgg gtgcctggcc tttaagcttc gcgaacagat caccgcgctc
     3301 gccggtggtg aacgcggcct tgcagtttgc atcggtcagg cgataggcga tctcgctctc
     3361 ggttgcgtca tagctgatcg gtacgccgat cgcccccgcc cgcgtgatcg caaagcacgc
     3421 ctcaacccac tgcaccgaat tcggcagcat gatcgcaaca gtgtcgttcg cctctatccc
     3481 gttgtcggcg agatgcccgg cgagcctgcc ggtgcgatcg aggagttcgg cataggtcac
     3541 cgacgaccgc gagtcgcggt acgccgtttt gcttccgcgc attgcggcgt gtcgtttcag
     3601 caattccggg atgggcgcga tcagatccgt atgcagc
//
LOCUS       DASSRL010000097         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1647255, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000097 DASSRL010000000
VERSION     DASSRL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647255"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..556
                     /locus_tag="VFL49_02040"
     CDS             194..556
                     /locus_tag="VFL49_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HET7910786.1"
                     /translation="MSVFGSIMSAIFGHGSAQASTSPTAPPPSAPPPQTVDVAAIVDK
                     AAAATGEKLAWRTSIVDLMKALNLDSSQAARKQLATELKYTGNMNDSAAMNVWLHKQI
                     MQKLAANGGKLPSDIKAA"
     gene            644..1225
                     /locus_tag="VFL49_02045"
     CDS             644..1225
                     /locus_tag="VFL49_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HET7910787.1"
                     /translation="MGLLDILNGMQNGPRGQTRPAPAGSGGMSPIAMALLGLVAYKAI
                     KHLSPDQATTAAPTGRGASTAGGTSAGGPGGPSAGGLGGLLQGPLGGLLAGGAAGTVL
                     SGGLNDLLKQFQQGGQSDVVHSWIGTGPNKSISPDVLSKVLSPDQIRTLSAQSGMSRD
                     DLMATLSEQLPELINQLTPDGRIPNEHEASRMI"
     gene            1263..1541
                     /locus_tag="VFL49_02050"
     CDS             1263..1541
                     /locus_tag="VFL49_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7910788.1"
                     /translation="MGGILWIILIGFVAGMIARFLAPGPNNPSGFILTVVLGIAGSFL
                     ATFIGQAVGWYRADQGAGFIGATVGAILVLFIWNRLVARGVISDPGAR"
     gene            2037..2273
                     /locus_tag="VFL49_02055"
     CDS             2037..2273
                     /locus_tag="VFL49_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910789.1"
                     /translation="MAIYKLIANGSFGQKEIELMSAAYESALIDLGITNRDDQITELI
                     AKAIVNVVATGERDPGLIEERAINALGLRRIDAA"
     gene            complement(2327..>2967)
                     /locus_tag="VFL49_02060"
     CDS             complement(2327..>2967)
                     /locus_tag="VFL49_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7910790.1"
                     /translation="QLRRILADGSAIVVDARPRAEFDAGHIPGARVLDAPSGDEIAAM
                     ERLANGNKATSLVVYCNGPYCQASRRLAEKLAAAGFTNVRRYQLGMPVWRALGGPTVI
                     ELNGIIRIFKADRTAVFIDARPAEDFARGSLPGARNLTAGSLDNTLKQMMSGKLTNPP
                     LPLDDFNRRIVLFGHDSAQVLGLAMAMSKRPWHNVTYFPGSYEELAAGLRQK"
BASE COUNT          599 a          913 c          892 g          563 t
ORIGIN      
        1 aggtatcgca agcgaatgcg gtaaccagtg ccgcatgcga cgcgaatcct gttgcgccgc
       61 ttttccccgc agcaaataat tattgctgcc atcgagcatt caaaaagaag ctttggacga
      121 atttcgcggc cgtagggccg atgtctcttt tgatcaaata gccgctcgct agcggccaat
      181 atggaggaat gtcatgagtg tcttcggcag cattatgagc gcgatcttcg gccacggctc
      241 ggcacaggca agcacgtcac cgacggctcc gcctccaagc gcgcctccgc cgcaaacggt
      301 ggacgtagcg gcaatcgtcg acaaggcagc ggcggccaca ggcgagaagc ttgcgtggcg
      361 cacgtccatt gtcgacctga tgaaggcgct gaacctcgac agcagccagg cggcacgcaa
      421 gcagcttgcc accgagctca aatacaccgg caacatgaac gactcggctg cgatgaacgt
      481 ctggctgcac aagcagatca tgcagaaact tgcagccaat ggcggaaagc tgccgagcga
      541 catcaaggcg gcttgacgac gctctcggca acgagcgggc ggacccgcac gcggaccgca
      601 aacgacagtt gaaatatcgg ctgggttcat taggagaccg gcaatgggac ttcttgatat
      661 cctgaatgga atgcaaaacg gcccgcgtgg ccagaccagg cccgctcccg ccggcagcgg
      721 aggcatgtcg ccgatcgcga tggccctgct cgggctcgtc gcttacaagg cgatcaagca
      781 cttgagcccg gatcaggcaa caacggccgc gccgaccggc cgcggcgcat cgacagcggg
      841 cggcacgtcg gccggcggac ctggcggtcc atcggctggc ggacttggcg gattgcttca
      901 aggtcctctg ggcggtcttc tcgcaggggg cgccgccggc accgtgctga gtggcgggct
      961 taacgacctg ctcaagcagt ttcagcaggg cggccaaagc gacgtcgtcc attcctggat
     1021 cggcacgggt cccaacaagt cgatttcacc cgacgtcctg tcgaaagtgc tcagccccga
     1081 tcagatcaga acgctcagtg cccaaagcgg catgtcgcgt gacgacctga tggcgaccct
     1141 gagcgagcag ttgccggaat tgatcaacca gctcactccg gacggccgca ttccgaacga
     1201 gcacgaagcg tcacgcatga tttaggccac cgcattttgc ggttctacaa cgggagtcct
     1261 gcatgggcgg catcctgtgg atcattctta tcggctttgt ggcgggcatg atcgcccggt
     1321 ttctcgctcc cggcccgaat aatccttccg ggtttattct cacggtcgtt ctcggcatcg
     1381 cgggatcatt tctcgcgaca ttcatcggtc aggcggtcgg ctggtatcgc gccgaccagg
     1441 gagccgggtt catcggtgct accgtcggcg ccatcctcgt tctgtttata tggaatcgac
     1501 tcgtggcacg cggcgtcatc tcggatcccg gcgcgcgtta gcgcgattcc gaggcaatcg
     1561 ccgtgccgcc acgcgatggc attgcgtcac gtcgctactg gccggcgcat tattgggcga
     1621 caggaagctt tgatcgctca ccgtaaagct ttgggacaca acaccgcaga ggccgaaaac
     1681 cttttcagcg cattcctgcg atcagagcag acatttggac gacccggccg cgatcgttca
     1741 ccggcgccgg catccgtagc cgcgcctata gacgcgtctc gaaacctcga ctacgctggc
     1801 tttcgtcacc ggggggtgcg tgtgctggag gctgagtgcg atgccgcaag cccgtaaatt
     1861 tgccccgatg aaacctccga tcggtgagcg ccggtgtcca tcttgcggac tgccgatgtt
     1921 gctttcgcgc atcgagcctt ctgacgaaat agaccgcgac gagcgaacct tcgagtgttc
     1981 gcgttgtgcc tacgcggaaa ccacgaccgt caaattccgc taggcgcgga ggcgctatgg
     2041 ccatctacaa gctgattgcc aacggttctt ttgggcagaa agaaattgag ctcatgagcg
     2101 cggcctatga gagcgccttg attgatctcg gcataaccaa tcgcgatgac cagattaccg
     2161 aactgattgc caaggcgatc gtcaacgttg tggcgacggg cgagcgcgat cccggactga
     2221 tagaggaacg cgcgatcaat gcgctgggcc ttcgccggat tgatgccgcg taagaaatcg
     2281 cgttactcgc aaatagacca tttgctcacg cccgtcgctg caattcctat ttctgccgca
     2341 agccggctgc cagctcctca tacgatccag gaaagtaggt cacgttgtgc cagggccgct
     2401 tgctcatggc catggcaagc ccaaggacct gcgcggaatc gtgcccgaac agcacgattc
     2461 gccggttgaa atcgtcgagc ggcaacggcg gattcgtcag cttgccggac atcatctgct
     2521 tgagcgtgtt atcgagtgat ccggcggtca aattgcgggc acccggcaag cttcctcttg
     2581 cgaagtcctc tgcaggccgc gcatcgatga acacggccgt ccgatccgcc ttgaagatgc
     2641 ggataatgcc attgagctca attaccgtcg gcccgccgag agcgcgccac actggcatgc
     2701 cgagctgata gcgccgcaca ttggtgaaac ctgccgctgc cagtttttcg gcaagccgcc
     2761 ggctcgcctg gcaataggga ccattacagt agaccacaag cgaggtggcc ttgtttccgt
     2821 tcgcgagccg ctccatggcg gcaatctcat cgccggatgg cgcatcgaga acgcgcgcgc
     2881 ccgggatatg accggcatcg aactcagcac gcggccgcgc atcgacgacg atggcgcttc
     2941 catccgcaag aatgcgccgc agttgct
//
LOCUS       DASSRL010000098         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_300030, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000098 DASSRL010000000
VERSION     DASSRL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300030"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFL49_02065"
     CDS             <1..841
                     /locus_tag="VFL49_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HET7910791.1"
                     /translation="CADPIGDHAHSPVEGIVHRYPDRVLLKLTHVCAVYCRFCFRREM
                     VGPNKPAGLSPSALTAALDYIRSHREIWEVILTGGDPLILSPRRLRAVMKSLAVISHV
                     RVVRIHTRIPIADPQRVTGALVQALKIKGKPTYVAVHVNHPRELTVQARAACARMADA
                     GIPLLSQTVLLAGVNDAADVMAALMRALVECRIKPYYLHHGDLAPGTSHLRTDIATGQ
                     ELMRGLRGRVSGLCQPTYMLDIPGGHGKSPIGPNYVRWCGSDATQVVVDDFNGRRHRY
                     LAR"
     gene            complement(844..993)
                     /locus_tag="VFL49_02070"
     CDS             complement(844..993)
                     /locus_tag="VFL49_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910792.1"
                     /translation="MIMIKNKHVGEALRRDVVFVWHAVIAVVFIGFASLAVAAALLDL
                     LSFRH"
     gene            complement(1211..1286)
                     /locus_tag="VFL49_02075"
     tRNA            complement(1211..1286)
                     /locus_tag="VFL49_02075"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1250..1252),aa:Glu,seq:ttc)
     gene            complement(1383..1838)
                     /locus_tag="VFL49_02080"
     CDS             complement(1383..1838)
                     /locus_tag="VFL49_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7910793.1"
                     /translation="MRYLHTMLRVRDLDAALDFYCNKLGLKETRRRVDDKNRYTLVFL
                     AAPGDEKMVEESRKTGRPGLEVELTYNWDAEDYGEARYFGHLAYEVDDIYETCDRLMK
                     AGVTINRPPRDGVMAFVRSPDRHSIELLQKGSPKPPQEPWKSMGNTGKW"
     gene            1883..2644
                     /locus_tag="VFL49_02085"
     CDS             1883..2644
                     /locus_tag="VFL49_02085"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET7910794.1"
                     /translation="MIAPDLPTAISGLRDLIEEAQVIVPFTGAGISTECGIPDFRSPG
                     GLWTKNQPIPFDAFMADREMRDESWRRRFAMEDKFGGARPGRGHRALASLYRAGKVPG
                     LITQNIDNLHQASGIAPKDIVELHGNTTYATCLDCGERYELSWVRKKFDAADSRAPDC
                     ACGGYIKTATVSFGQAMPAEAMQRAEKLARNCRLFLAVGSSLVVWPAAGFPLTAKRAG
                     ATLVIINREPTDFDDVADLVVRNDIGDVLGPFITH"
     gene            2816..3391
                     /locus_tag="VFL49_02090"
     CDS             2816..3391
                     /locus_tag="VFL49_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7910795.1"
                     /translation="MSGTNLRPETVRDLVDETATDLIELSGVIKWFDVSKGFGFIVPD
                     NGMPDVLLHVTCLRRDGYQTAYEGARVICEVLQRSKGLQAFRVVSMDESTAVHPAQMP
                     PPRTHVTVTPTSSLERAQVKWFNRLRGFGFLTCGEGTPDIFVHMETLRRFGMTELRPG
                     QYVLVRFGPGSKGMMAAEIHPETGPSALSSH"
BASE COUNT          646 a         1019 c         1065 g          685 t
ORIGIN      
        1 atgcgctgac ccaatcgggg accacgcgca tagtccggtc gaaggtatcg tgcaccgcta
       61 tcccgaccgc gtgctgctca agctgacgca cgtctgcgcg gtctattgcc gcttttgctt
      121 ccgccgggaa atggtcggtc ccaataagcc ggccggactg tcgccgtcag cattgacggc
      181 tgcgctcgac tacatccggt cgcatcggga aatttgggag gttattctga ccggtggcga
      241 tccgctcatt ttgtcgccgc ggcgcctgcg cgcggtgatg aagagccttg ccgtgattag
      301 ccacgtcagg gtggtgcgaa ttcacacccg cataccgatc gccgatccgc agcgagtgac
      361 tggcgctctg gtgcaagcct taaagatcaa gggcaagccc acttatgtcg ccgtgcacgt
      421 caatcatccg cgtgagctga ccgtgcaggc gcgcgcggca tgtgcgcgga tggccgatgc
      481 cggcattccg ttgctgtcac agacagtgct cctcgccggc gtcaacgacg cagcggacgt
      541 gatggccgcg ttgatgcgtg ctttggtcga gtgccggatc aagccgtatt acttgcacca
      601 cggcgatctc gcgccgggca ccagtcatct tcgcaccgat atcgcgaccg ggcaggagct
      661 gatgcgcggc ctgcgcggcc gggtatcagg cctttgtcag ccgacttata tgctcgatat
      721 tcccggcggg cacggcaagt cgccgatcgg accaaactat gtccggtggt gcggttcaga
      781 tgcgacgcag gtcgtcgtcg acgacttcaa cggcaggcgg caccgctacc tggcgcgcta
      841 gaatcagtgt cggaaggaca gcaggtcgag cagggcggcc gcgaccgcga gactcgcaaa
      901 gccgataaac acaaccgcaa tgactgcatg ccaaacgaag acgacatcgc ggcgcagtgc
      961 ctcgccgaca tgcttgttct tgatcatgat catggcctgt cgctccaggc agagccgttc
     1021 gcaggacgtt ttattttaga cgttatgaat caccggcccc ataacttgtg cgttaaaaag
     1081 cgcgtgaaaa agtgccgcac ctgtgcatgg cagccatgag gaagcggtgg tatcggcatg
     1141 acttttgaac ttggaatgaa tttgaaacgc cggcgccacc gattttgcga aattttgatg
     1201 tgaaaaaatc tggcgcgccc taggggattc gaacccctgt taccgccgtg aaagggcggt
     1261 gtcctaggcc tctagacgaa gggcgcttgg ccgtcgaccg gccacagggc atccgccggg
     1321 ccgaacgaac aaaggtttgg ggggtataaa ggcgttcgcc gcgagcggca agcgaatgcg
     1381 cgctaccatt ttcccgtatt tcccatcgat ttccatggtt cctgcggcgg cttcggcgag
     1441 cccttctgca gcagttcaat cgagtgcctg tcgggcgagc gcacaaaggc catgacgccg
     1501 tcccgcggtg gccggttgat ggtgacgccc gctttcatca ggcggtcaca ggtctcgtaa
     1561 atgtcgtcga cctcgtaggc gagatggccg aaataccgcg cctcgccgta gtcttcggca
     1621 tcccagttgt aggtgagttc gacttccaat cccggccgtc cggttttcct gctctcctcg
     1681 accatctttt cgtcgccggg cgcggccagg aacaccagcg tataccggtt cttgtcgtcg
     1741 acgcgccggc gcgtctcctt caggccaagc ttgttgcaat aaaaatcaag cgcggcgtcc
     1801 aggtcgcgga cgcgaagcat ggtgtgcagg tatcgcatgg gctaaacctc gctgactgac
     1861 aggcggcacc cattatatag ctatgatcgc gcccgatctt ccaaccgcaa tctcgggcct
     1921 ccgcgacctg atcgaggagg cgcaagtgat cgttccattt accggggcgg ggatttccac
     1981 cgaatgcggg attccggact ttcgctctcc cggcgggcta tggacgaaga accagccgat
     2041 cccattcgac gccttcatgg cggatcgcga gatgcgggac gagtcctggc gccggcgttt
     2101 cgccatggaa gacaaattcg gcggggccag acccggtcgt ggtcatcggg cgcttgcgag
     2161 tctctatcgt gccggcaagg tgccgggcct gatcacgcag aatattgaca atctccatca
     2221 ggcttcgggc atcgcaccga aagacattgt cgagttgcac ggcaacacga catacgccac
     2281 gtgtctcgac tgcggcgagc gctatgagct gtcgtgggtg aggaagaaat tcgacgccgc
     2341 agacagtcgc gcgccggact gtgcgtgcgg cggctacatc aagacggcga ccgtttcgtt
     2401 cggccaggcg atgccggccg aagccatgca gcgggctgag aaattagcca ggaattgcag
     2461 gcttttcctc gctgtcggct cctcgctggt ggtatggccg gcggcaggat ttcctctgac
     2521 ggcgaagcga gccggcgcaa cgctcgtcat catcaatcgc gagccgaccg acttcgacga
     2581 tgttgccgat ctggtcgtgc gcaacgacat cggtgacgtg ctcggaccct ttatcaccca
     2641 ctgattcgga ctgcgctgcg ctgtgcacag agctgtgtgc aagcccattt tgccctttgc
     2701 ggttcgaagg tggatgttaa attcgattcc acaggattcg ttgaagcgct cttgaagagg
     2761 cgccaggaag acggcggcgt gcggcttcat ggcggcggac gggatcgaag cgaaaatgtc
     2821 tgggaccaat ctccgaccag agacggtcag agatctcgtc gacgaaacgg cgaccgatct
     2881 catcgaattg tctggcgtca tcaagtggtt cgatgtctcg aagggattcg ggttcatcgt
     2941 ccccgacaat ggaatgcccg acgttctttt gcatgtgacg tgcctgcgcc gcgacggata
     3001 ccagaccgcc tatgagggcg cgcgggtcat ctgcgaggtc ctgcaacgat cgaaaggtct
     3061 gcaggcattc cgggtcgtgt ccatggacga gtcgactgcg gtgcatccgg cgcaaatgcc
     3121 gcccccgcgg acgcatgtca cggtcacccc aaccagcagc cttgagcggg cgcaggtcaa
     3181 atggttcaac cggctgcgcg gcttcggctt cctcacttgc ggcgagggga cgccggacat
     3241 cttcgtgcat atggagacgc tgcgccgctt cggcatgacg gagcttcgcc ccggccagta
     3301 tgtgctggtc cgcttcgggc ccggctccaa gggcatgatg gcagccgaga tccatccgga
     3361 gaccggaccg tcggcgctgt cttcgcattg atccagcact gatcgcattg acgga
//
LOCUS       DASSRL010000099         5347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1174930, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000099 DASSRL010000000
VERSION     DASSRL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174930"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1134
                     /gene="holA"
                     /locus_tag="VFL49_02095"
     CDS             106..1134
                     /gene="holA"
                     /locus_tag="VFL49_02095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7910796.1"
                     /translation="MTAIKAADVDRFIAKPDGSQSIVLVFGPDAGLVRERVDALVRGS
                     VDDSTDPFTFVRIDGDELAGNPSRLVEEAHTVPLFGGRRAVLVKAGSRNIAPALETVI
                     AAPSTECRIIIEAGNLGKSAPLRALCEKAKTAAALPCYADNARALTQLIDEEVRAAGL
                     TIEGNARAALIGLLGSDRLSSRNEIRKLTLYARGKKSIELADVTAAVADASEIALDAV
                     IDAAFAGRTGEVDSEYFKARAGGSSPTAIVSAAIRQVASLHKMILAVEHGDTFEFAMK
                     RAAPPVHFTRESKVGEALRAWTPPRLLRAMEQLAEASLDMRRNAPLADTIAQRTLLSL
                     AMSARKRT"
     gene            1345..1659
                     /locus_tag="VFL49_02100"
     CDS             1345..1659
                     /locus_tag="VFL49_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910797.1"
                     /translation="MTIVTAFFLVVFGASLFLGTVMVIGTFGGDAASELTANGRAGRI
                     ARTLQDGTLCHYMIFDNRTAQTAEDRIGRCDENKTKPKQERPTTFSWGNNRRRMIDGP
                     IN"
     gene            complement(1649..2554)
                     /locus_tag="VFL49_02105"
     CDS             complement(1649..2554)
                     /locus_tag="VFL49_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7910798.1"
                     /translation="MAASLHGELQPTIYRFRLGDFEVATVLDAKAVRDGLHPHYGANA
                     SVDDVHALARANNIDTQRFEHPNIPTIVNTGKQLVLFDTGNGALPREYEQLSKRLPPG
                     QTAGRLAAAGYKPEDIDVIVLTHGHPDHIGGLTEGGKPVFPKARYVFGAAEFDFWKRG
                     ENVREARKFNRELYVKICLPLADRSTFIKPGDDVVPGITAVDAAGHSPGLLAFHIESN
                     GKRIMITADTFTQYVMAVQRPEWHFEMDDDKDKAVATRKRMLDMLATDKLFIASFHMP
                     FPGIGFVEKGQGGYRWVPHSYQLNL"
     gene            3013..4095
                     /locus_tag="VFL49_02110"
     CDS             3013..4095
                     /locus_tag="VFL49_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7910799.1"
                     /translation="MAPPALFQPLNAIRNLLSARRGQALITSLFAVAFLFAMVWDNLE
                     TSFSAAISSMQADANAGSVVLSFTHAGSSAAGDSELVFLVDFDTGAQICELDSSAAIS
                     HLDLATINDNAMTGFVSLRVNPGARQSAGAIFADTDNTSTNLTSYTRRESLSNFATAF
                     RSIRKLAAMVGLPRIQEAAALSRTMLIGTVSTYNPYRDGKEEGGVRTASGEFYDPTAW
                     TAAVQTALRNQFGGVRYGRLYQPVYALIARGEKQLIVKINDVGPLRPGRVLDLNERSM
                     RYFDPFLTQGLIQDVRITLLPGEDWTPGPVGEAYAIDFAAARRPLPVSLANEEAELAN
                     MRARLGHVTEPDMKSDAHAPVKLSGG"
     gene            complement(4120..4995)
                     /locus_tag="VFL49_02115"
     CDS             complement(4120..4995)
                     /locus_tag="VFL49_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7910800.1"
                     /translation="MADHSRLGRGLAALIGDVGDETPAADGGRKPRRAPIENLQPNPR
                     NPRRLFTDEELDELATSIRERGIIQPIVVRALKGDSFEIIAGERRWRAAQRAGLHDVP
                     IAVVEANDAQSLEYAIIENVQRADLNPIEEATGYLALMDDFNRTQEDVAQIVGKSRPH
                     IANTIRLLKLSEPVKQLVRSRKISAGHARLLVGQSNGELIAQEIIDKGWSVRDLEANM
                     RKDVTAQIRDRSREAKGAAAGVSKDADIRALERRLSDALGLDVSVEHRGESGTLRIKY
                     RNLEQLDAVLRKLGA"
     gene            complement(5071..>5347)
                     /locus_tag="VFL49_02120"
     CDS             complement(5071..>5347)
                     /locus_tag="VFL49_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7910801.1"
                     /translation="KTTLNPTLSIHGIVLTMYDSRNNLSNQVVADVRQFMGSKVYETI
                     IPRNVRISEAPSYGKPVLVYDLKCVGSEAYLRLATEIIQREKALKAG"
BASE COUNT         1124 a         1563 c         1487 g         1173 t
ORIGIN      
        1 gccaaggaaa ttgccgaaaa cattcggaac cggttggcct cgcatttcgc agcgggcacc
       61 tgacggtgac tttttcgcgc cagccggcca ggtgatgacg cgccgatgac cgccatcaaa
      121 gccgccgacg tcgaccgctt catcgccaaa ccggacggca gccaatcgat cgtgctcgta
      181 ttcgggcctg atgcgggctt ggtccgagaa cgcgtggacg ccctggtgcg aggatccgtc
      241 gacgactcca ccgatccatt cacattcgtg cgcatcgacg gcgacgagct tgccggcaat
      301 ccgtcccggc tggtggagga agcccacacg gtcccgctgt ttggaggccg gcgcgccgtc
      361 ctggtgaagg cgggatcgcg gaacattgcg cccgctcttg agaccgtcat tgcggctccg
      421 tcgactgaat gccgcatcat catcgaggcg ggcaatctcg gcaaaagcgc gccgttgcgc
      481 gcgctctgtg agaaagcgaa gacagcagcc gcacttccct gctacgccga caatgcacgc
      541 gccttgaccc aactcatcga cgaggaagtg cgcgcagccg gcctgacgat cgaaggcaat
      601 gctcgcgcgg ctttgatcgg attgttgggc agcgaccggc tttcgtcacg taacgaaatc
      661 cgcaagctga cgctctatgc gcgcggaaag aaaagcatcg aactcgcgga cgtcaccgcg
      721 gcggtcgccg acgcttccga gattgcgctt gacgccgtta tcgacgccgc ttttgccggt
      781 cgaaccggag aggtcgacag cgaatacttc aaagcacgcg ccggcggctc ttctccgacg
      841 gccatagttt ccgccgcaat ccgacaggtt gcgagcctgc ataaaatgat attggcggtc
      901 gagcacggcg atactttcga gtttgcaatg aaacgcgccg ccccgcccgt gcacttcacg
      961 cgcgagagca aagtgggcga agcgctgcgc gcatggacgc ctccccggct cctgcgcgcc
     1021 atggagcaac tggcagaagc ttcccttgac atgcggcgca atgcaccttt ggcggatacg
     1081 attgcacagc gcactctgct ctcgcttgca atgagcgccc ggaaaaggac ctagattctg
     1141 ccgcccgccc ttacttcacg cttaaatcgc attaccagcg taacgccaat gtgaagcgtc
     1201 gcctttagaa ctgatcaagg ctttttcttt aaattttcca acgcgagtct atcccaattc
     1261 aaaaagcggc attcgaaaat gcagagtttc gaggcccggc gagttttcag tcgttcgaat
     1321 gaggcacgcg cccagttctg gcacgtgaca atcgtcacgg cgtttttcct tgttgtattc
     1381 ggtgcaagtt tattcctcgg taccgtcatg gtgattggca cattcggcgg cgacgccgct
     1441 agtgaattga cggccaatgg gcgcgcgggt cgcattgcgc gcactttgca ggacggcacg
     1501 ctgtgccatt acatgatctt cgacaatagg acagcacaga cggccgaaga ccgcatcggc
     1561 cgctgcgatg aaaacaaaac caagccaaag caggaaaggc cgacgacgtt cagctgggga
     1621 aataatcgtc gccgaatgat cgacggccct ataaattgag ctgataactg tgcggcaccc
     1681 agcgatagcc gccctgccct ttttccacaa agcctatccc gggaaacggc atgtgaaagc
     1741 ttgcgataaa caacttgtcg gtcgcaagca tgtcgagcat tcgtttgcga gtagcgacag
     1801 ccttgtcctt gtcgtcatcc atttcgaaat gccactccgg ccgctgcacc gccattacat
     1861 actgggtaaa tgtatcggcg gtgatcatga tccgcttgcc gttgctttcg atgtgaaagg
     1921 caagcaatcc gggagaatgg ccggcggcat caacggccgt aatgccggga acaacatcgt
     1981 caccaggctt gataaacgtc gaccggtcgg ccaagggcag gcatattttc acatagagct
     2041 ctcgattgaa tttccgcgcc tcccggacgt tttcgccgcg cttccagaag tcaaattctg
     2101 cagcgccaaa aacataacgc gcttttggaa acaccggctt accgccctcg gtcagtccgc
     2161 cgatgtgatc aggatgtcca tgcgtaagga caatcacgtc gatgtcctcc ggcttgtagc
     2221 cggcagcggc caaccgcccg gcggtttggc cgggcggcaa ccgtttgctg agttgctcgt
     2281 attcgcgcgg caatgcgcca ttgccggtgt cgaacaagac aagctgctta ccggtgttca
     2341 cgattgttgg aatgttcggg tgctcaaacc gctgggtgtc gatattgttg gcgcgtgcca
     2401 gggcgtgcac gtcatctacc gaggcattgg ccccatagtg tggatgcagc ccgtctcgga
     2461 cggccttggc atccaaaacc gtggcgactt cgaaatcgcc aagcctgaaa cgatagatcg
     2521 tcggctgcaa ttcgccgtgc agtgatgctg ccactgatag tcctccccag tatcgtcgcc
     2581 tcattcatgc ttagcagctg acgcagagac ggcaagcaat ccggacgcaa catttagcga
     2641 ttctgttatg cggcagagtc tggaccttta gtccacacaa taggaaaata taccgcttca
     2701 gtcctattgc agcgagtgag cattttcact cattttgcct gaatgcgttc acgatcggtg
     2761 cattattcaa aacgaaaaga gggacaggtt ctccgaccaa tgcgtcgtcc gtacgcagga
     2821 cgaccgccaa gtttttcgag ccgattgtgc attgtgggct tgatggacgg gagttccctt
     2881 ttcgcaacag cggttctcgc attgaacatt tgcataaaac tctaagagta atttcgcgaa
     2941 gcattttacg cgcgttttat cagccatcgc tacagcattg cgtcgaaaat tattcaactg
     3001 ccgacggtgt tgatggcccc gccagcgttg tttcaaccgc tcaatgcgat ccggaatctg
     3061 ctttccgcaa ggcgcggaca agcgttgatc acatctttgt ttgctgtcgc cttcttgttc
     3121 gcaatggtgt gggacaatct cgagaccagt ttctctgcag cgataagctc gatgcaggcg
     3181 gatgccaatg ccggctccgt cgtattgtcg ttcacgcatg ctgggtcttc ggcagccggc
     3241 gattccgagc tggtttttct ggtcgatttc gataccggcg ctcaaatatg cgaactggac
     3301 agctcggcag cgatatcgca tctcgatctt gcaacgataa acgataatgc aatgaccggt
     3361 ttcgtttcgc tgcgtgtgaa tcctggcgcc cgtcaatcag cgggcgctat ttttgcggat
     3421 accgataaca ccagcacaaa cctgacgtcc tacacgcgca gggaatcgct ctcgaacttt
     3481 gcgacagcat ttcgatcgat ccggaaattg gccgcgatgg tcggccttcc gcgcatccag
     3541 gaagcagcag ccctttcccg cacaatgctc atcggcaccg tttcaaccta caatccttat
     3601 cgcgacggca aggaggaagg cggagttcgg acggcttcgg gcgagtttta cgaccccacg
     3661 gcatggaccg cggcagtcca gaccgcgttg cgcaatcagt ttggcggcgt acgttatggc
     3721 aggctctacc agccggtcta cgccctcatt gcgcgtggcg aaaagcaatt gattgtcaaa
     3781 atcaacgacg tgggaccact tagaccgggc cgcgtccttg atcttaatga acgcagcatg
     3841 cgctatttcg atcctttcct cacacaaggc cttattcagg acgtcagaat tactctcttg
     3901 ccgggcgaag attggacgcc tgggccagtg ggcgaggcct atgccatcga ttttgctgcc
     3961 gcgaggcgac cgctgcctgt ctctttggcg aatgaagagg ccgaactcgc caatatgcgc
     4021 gccaggttgg gtcatgtgac cgagcccgac atgaagtccg atgcgcacgc tccagtaaag
     4081 ctgagtggtg gctgaaccgg cgatcaagcc gaaaaaatgc taagctccga gtttcctcag
     4141 cactgcatcg agttgttcca gatttcgata cttgatacgc aaggtcccac tttcgccgcg
     4201 gtgctcgacg ctgacgtcga gtccaagggc atcggaaagg cgccgctcaa gcgcacggat
     4261 gtcggcgtct ttggaaacac cggctgcggc gcctttggct tcgcgactcc tatcgcgaat
     4321 ttgagccgtg acgtccttgc gcatgttggc ttcgaggtcg cgtacgctcc agcccttgtc
     4381 gatgatctcc tgtgcgatca attcgccatt ggattgtccg accaaaagcc gagcatgccc
     4441 tgcggagatc ttgcgcgaac gaaccaattg cttcacgggc tcagataatt tcagcagccg
     4501 gatcgtattg gcaatatgcg gccgactctt gccgacgatc tgcgcaacgt cttcctgcgt
     4561 gcgattgaaa tcatccataa gtgcaagata accggtcgct tcctcgatcg gattgagatc
     4621 ggcgcgttgc acgttttcga taatggcgta ctcaagagac tgggcatcgt ttgcttcgac
     4681 cactgcgatc ggcacgtcgt gaagaccagc gcgttgcgcc gcgcgccacc gccgctcccc
     4741 ggcgatgatc tcgaagctat cgcccttgag cgcgcgcacg acaataggct gaataattcc
     4801 acgctcacgg atcgaggtgg caagctcgtc caactcttca tcggtgaaca gccgccgcgg
     4861 attgcgcggg ttgggctgca gattttcgat cggcgcacga cgaggcttgc gtccaccgtc
     4921 ggcggccggt gtttcatcac ccacatcacc gatcagcgca gcaagaccgc gtcctaatcg
     4981 tgagtgatca gccattgccg ctcctgaatt gcgtgccacg ctatcccttt ccgcgcttat
     5041 cgactattcc gattcacatt cgatcgtgcg ctagcctgcc ttcaaagcct tttcgcgctg
     5101 gatgatttcg gtcgcaagac gcagataggc ttcgctgccg acacacttga ggtcataaac
     5161 gagcaccggc ttaccgtaag acggcgcctc cgagatgcgc acgttgcgtg ggattatagt
     5221 ctcatagact ttgctgccca tgaactggcg cacatcggcg acaacttgat tcgagagatt
     5281 gttgcgcgag tcgtacatcg tcagcacgat gccgtggatc gacagtgtcg gattgagagt
     5341 ggtcttg
//
LOCUS       DASSRL010000100         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1717179, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000100 DASSRL010000000
VERSION     DASSRL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717179"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFL49_02125"
     CDS             complement(<1..469)
                     /locus_tag="VFL49_02125"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910802.1"
                     /translation="MQSNAESYAAFEKELGGALIRAGRLDARGLDRALRLRSSGSDSL
                     IDLLPRLGLISERDLADAIARHLGLPLVASRDYPELPILEDKISARFLRDAHVLPLSV
                     ETDGVSVAMADPLNRFAVDAIHLVTGLRVKPRVAVPAELEAAIDRLHGRDQAAA"
     gene            complement(556..1290)
                     /locus_tag="VFL49_02130"
     CDS             complement(556..1290)
                     /locus_tag="VFL49_02130"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET7910803.1"
                     /translation="MLLILGAPFIGSFLGVVAERWPKGDTFVFGRSRCLHCRHVLGLR
                     DLIPVISWLASRAKCRYCGQRIPIRYPIIEICALALAIWPVVELPAELAWISAMFGWT
                     LLTLALIDLRWLWLPQALTAPLAAVGLLVAGLWRSDIPFDQLIGAVAGFLVMTGVAWL
                     YRRCRGREGLGEGDAGLFGALGAWVGWQGLPTVLLYAGISGLLLLAMQRTTGRVVKLT
                     TRLAFGPHLCLGGWLVWLYGPLYLDW"
     gene            complement(1782..1997)
                     /locus_tag="VFL49_02135"
     CDS             complement(1782..1997)
                     /locus_tag="VFL49_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910804.1"
                     /translation="MEIRPRILDCPARDEHIVRRLGKAVVVQWDALPGPVQDLILKQA
                     VLMHDQNRVLQLRHKIKAFIQDWKIVE"
BASE COUNT          647 a          856 c          801 g          527 t
ORIGIN      
        1 cggcagccgc ttgatcgcgg ccatgaagcc ggtcgatcgc cgcctcgagt tctgccggca
       61 cggcgacccg cggttttacc ctcagcccgg tcacgagatg gatggcatcc actgcaaagc
      121 gattaagcgg gtccgccatg gcgacggaga cgccatcggt ctccacggac aacggcaaga
      181 catgcgcgtc tctcaggaag cgggcgctga tcttgtcttc gaggatgggc agttcaggat
      241 agtcgcgcga cgcgaccagc ggcaggccaa gatgccgggc aattgcatcc gccaggtcgc
      301 gctcggagat aagaccaagc cgtggcagca gatcgatgag actgtcggat ccgctggatc
      361 gcagccgaag tgcccggtcg agacctcgcg cgtcaagcct gcccgcacgg atgagcgctc
      421 cacccagctc cttttcgaat gcagcatagc tttcggcgtt ggactgcatg tccggtgctc
      481 aatgctttcg cacggccgcc gggagcatag cagaaaacag gcggcgcaac aaaaatcggc
      541 cagcgcgccg cgcagtcacc aatcgaggta gagaggaccg tagagccaca ccagccagcc
      601 gcccaagcac agatgcggcc cgaaggccaa gcgtgtcgtc aacttgacga ctcggcccgt
      661 cgtcctctgc atcgccagaa gaagcaggcc actgatacct gcatagagca agaccgtagg
      721 aaggccttgc cagccaaccc aggcgcccag ggcaccaaac aggccagcat caccttcgcc
      781 caatccctct cggccgcgac agcggcggta cagccacgcc acgcctgtca tgaccagaaa
      841 gcccgcgacc gccccgatga gttgatcgaa cggaatgtcc gaacgccaca atccagcgac
      901 aaggaggccg accgcagcga ggggtgcggt caatgcctgg ggcagccaaa gccatctaag
      961 atcgataaga gcgagggtaa gaagagtcca accgaacatg gcactgatcc atgccagttc
     1021 tgctggcaac tcgaccaccg gccagattgc cagggcgaga gcgcagatct caatgatcgg
     1081 gtacctgatg ggaatccgct ggccgcagta gcggcacttt gcccgcgagg cgagccagct
     1141 tatgaccgga atgagatcac gcaagccgag gacgtgcctg caatgaagac agcgcgatcg
     1201 gccgaataca aacgtatcgc cctttggcca acgttcggca acaacgccca ggaagctgcc
     1261 gatgaaggga gcacccagta tcaggagcaa cgggccatcc aacaatatgt gtagagggcc
     1321 catcagggtt tcatatccgg ccgatcgact gggcctttat cctgtgtgtg catgattcca
     1381 agccaacctg aaccgtccag ccttgcgaac ccctccggca tcgtatgagg cgcaatttcc
     1441 agtgttcgac aaatgccgaa acagcagaga tcgtaacggt tttcgtgcct tcgcggcgcc
     1501 ggagtggatt tccgcgcgga tctgggccga tgcaagcgca ttggaattgg atgtccgcaa
     1561 acaattatag gccgaaatcc agcggaaatg gacagtcatc gtgcccacac agcgactccg
     1621 caccggcctc tgggttcgcc gctgcggccg tgcaaccgct cggacggaat tcgacccatc
     1681 ggtcgcgtcg atctgcaggg atcgcggaag acaatcatct gtgcaagtcc ttcgctggaa
     1741 cggcctgata tctcatcggt gccgcaccat ggaagctgcc gctattcgac gatcttccaa
     1801 tcctggatga aagctttgat tttgtgccgc agctgaagga cccgattctg gtcatgcatc
     1861 aatacggcct gcttcaatat caggtcttgc accgggccgg gcaaagcgtc ccattgcacc
     1921 accaccgctt tgcccagccg acgaacaatg tgctcgtcgc gggccgggca atccaagatg
     1981 cgcggtctga tttccatcaa gcaatgcaac cactacacga gtgaaccgat catgaacgat
     2041 agtcagcgcc atgctggctg cgaagcatgc cccaaacgct gggggtttct cgccggaccc
     2101 atttcgcgcc ggcggtgcgc atggattttc gtcgtgtcgg atcacgatgc atctctttgt
     2161 aatgcacctc caccgcatcg cacgactgcc cgagagggct tgtcggcgca gtgtcaagcg
     2221 aacgggatga tcaacgcgct gatcatggac aagacgatca gcgaactgcc gatcgtatgg
     2281 tgacagtgtt ttcgctcagg atgtcgcggt aggagttgat atcccacatc gctttccttc
     2341 ctcgtcgatt tccggctctg tcgagaaggc cgctttggcg ctggtgctcg caggagcgat
     2401 tgcgcaccgc tgcgcaggtc ggccgcattt ctcacgggca tgtcacgtgg aggtaaattc
     2461 ctcgcgcatc cgcgcacaag aaaacgcgtt cccaagagcg gcacttccag gcggtccctg
     2521 acaagtatgt cagcaaatct ttttggcgcc gagaccgaac gaagaatttc gaccacaacc
     2581 aaattcctaa tctgtggata actaaagtaa acgctccgta atgcacgtga gcggcgataa
     2641 agcaatcgat cagtattatt cgaaataata ctttcgttat taccgaagaa accttcgtaa
     2701 atcggagcaa gcgcacgagc ttattactac tgaaacttga ctttaacgaa ttttaaccat
     2761 actattgtcg gcctccgtcc agcgacgcgt ttccgccggc ctctttttgt gggccagcgg
     2821 aatgtggctc g
//
LOCUS       DASSRL010000101         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1384867, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000101 DASSRL010000000
VERSION     DASSRL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384867"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFL49_02140"
     CDS             complement(<1..526)
                     /locus_tag="VFL49_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7910805.1"
                     /translation="MTASAKGRTYSARHLAVGLAGYCAFINLYSPQAILPLLSEEFHA
                     SAAEVSTIITVSTLAVALTAPFTGAVADVLGRKRVIVAAMFVLVIPTLMVGLSTSLSA
                     VIFWRAVQGLVLPPIFAVTVAYIGDEWPAREATTAAGIYSSGSSLGGFSGRLITGVIA
                     DLISWRAGFAVLAGV"
     gene            complement(640..2169)
                     /locus_tag="VFL49_02145"
     CDS             complement(640..2169)
                     /locus_tag="VFL49_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7910806.1"
                     /translation="MGDQAQQPVIDFLADADTYGGTAPVKRIDTHAAIVFLAGDRVLK
                     IKRAVRFPFLDFSTLGKRRAACEAEIKVNRAFAPSIYRGVVPITRDPAGRLAIAGKGD
                     PVEWAVEMRRFDESQTLDRLAESGHIDEALADRLGRTVAAAHRVAPTVTNAHFAETLA
                     NIIAQNETELATEPDLFSLEDLRALAATTRDAFDRVKPLLQARERAGLVRRCHGDLHL
                     GNVVLIDGKPVLFDAIEFDDRIATGDVLYDLAFVLMDLIERGLRPAANIVFNRYMIET
                     QRIHDLEALAALPLFMSVRAAIRAKVTAARRKHSNAREKLSRYARSYAELARELLKPA
                     TPKLVAIGGLSGTGKSLLARRLAPEILPMPGAVVLRSDVERKAMFGVAETERLPKSAY
                     AAEVTAKVYAALAAKARRVVAAGHSAIVDAVFAQPGERAGIANAAGSAGFVGLFLTAD
                     LQSRLDRVGARASDASDASAAVVQDQENFELGRINWAKVDASGTPEETFIRAKVALDL
                     E"
BASE COUNT          496 a          773 c          800 g          443 t
ORIGIN      
        1 cgacaccggc gagcacggca aatccggcac gccagctgat cagatcggca atcactccgg
       61 tgatcaggcg gccgctgaag cccccgagac tcgatcccga cgaataaatg ccggcggcgg
      121 tcgttgcttc cctggccggc cattcgtcac cgatataggc gacggtcacg gcaaagatcg
      181 gcggcaggac gagcccctgc acggcgcgcc agaagatgac ggccgacagg ctcgtcgaca
      241 gcccgaccat cagagtcggg atgaccagga cgaacatcgc cgcgacgatc acgcgcttgc
      301 gtccgagcac atcagccacc gcgccggtga atggcgcggt cagagcaacc gccagggtgc
      361 tgacggtgat gatggtcgag acttccgctg cgctggcgtg gaattcttcc gagagcagcg
      421 gaagaatcgc ctgcggcgaa tagagattga tgaaggcgca ataaccggca aggccgaccg
      481 cgagatgacg cgcggaatag gttcggcctt tggcacttgc ggtcataaag gaagacctgg
      541 cgggcgggta tgtttcaacg cttgcagcta tgccttggtt cgctgtcttt cgccaagagg
      601 ggagatggcg ggactgcggc tcgcgaatgc atagctttgt cactccaggt cgagcgcgac
      661 ctttgcacga atgaacgttt cttccggagt gccggaagca tcaaccttcg cccagttgat
      721 gcggcccagc tcgaagtttt cctgatcctg cacgacggct gcgctcgcat cggacgcgtc
      781 gctcgcgcgc gcgccgacgc gatcgagccg ggactgcaga tcggcggtga gaaataggcc
      841 cacaaagccg gcggaacccg cggcattcgc gatgccggcg cgctcgcctg gctgtgcaaa
      901 tacggcgtca acgatcgccg aatgccccgc tgcaaccaca cggcgtgcct tcgctgcaag
      961 cgcagcatag accttggcgg tgacctccgc ggcatatgcg gacttcggca gccgttcagt
     1021 ctcggcaact ccgaacatgg ccttgcgctc gacatcgctg cgcaacacca cggcgccggg
     1081 cattggcaag atttcaggcg caagcctgcg cgcgagcaag gactttccgg tacccgaaag
     1141 gccaccaatg gcgaccagtt tcggcgttgc aggcttaagc agttcgcgcg ccagttcggc
     1201 ataactgcgc gcgtagcgcg acagcttttc gcgcgcattg gaatgcttgc gtctagccgc
     1261 cgttaccttg gcgcggatgg cggcgcgcac cgacatgaac aggggaaggg cggcgagggc
     1321 ctcaagatca tggatacgtt gcgtctcgat catgtaacgg ttgaacacga tgttcgcggc
     1381 cggacggagc ccgcgctcga tcagatccat cagcacgaac gccaggtcgt atagcacgtc
     1441 gccggttgcg atgcggtcgt caaattcgat ggcgtcgaac aggacgggtt tgccgtcgat
     1501 cagcacgaca ttgccgaggt gcagatcacc atggcaccgg cgcacgagac ccgcacgctc
     1561 gcgcgcctgc agcaatggct tcacgcgatc gaacgcatca cgcgtagtgg cagccagggc
     1621 ccgcaggtcc tcgagggaaa aaaggtccgg ctccgtagcg agttcggtct cgttttgcgc
     1681 aataatgttc gccagcgttt ccgcaaagtg cgcgttcgtc accgttggcg cgacacgatg
     1741 cgcagcagcc accgtgcgtc cgagccgatc cgcgagcgcc tcgtcaatgt gcccggactc
     1801 ggcgagccga tcgagggtct ggctttcatc gaaacgtcgc atctcgacgg cccattccac
     1861 cgggtcgcct ttgccggcga tcgcaagccg tccggcggga tcgcgtgtga tgggcacgac
     1921 accccggtaa atcgacggcg caaatgcccg gttgaccttg atctcggctt cgcaggcggc
     1981 cctgcgtttg ccgagcgtgg aaaaatcgag aaagggaaag cgcacggcgc gcttgatctt
     2041 gagcacccgg tcgccggcaa ggaaaactat tgccgcgtgc gtgtcgatgc gcttgacggg
     2101 cgcggtgcca ccataggtat ccgcgtcggc cagaaaatcg attaccggct gttgtgcctg
     2161 gtcacccata cgtggctgct tgtcctgacc aatatgtatc tattcataac gataagagga
     2221 gacgcgcata ttcgggcact tcggtggaac tgggaacccg agagaagcgt tgttcaaaca
     2281 agcggggcgg cctcgcggcg tgacatctat gcgtgccctg accgttaatc ccagccaacc
     2341 tcattccctc agcctagaag aagtaccatc gccaccggaa agcgatgggg ccgtgctggt
     2401 gcgcgcaacg gctctcggca tttgcgggac tgaccgcgag atcattgagg gcctttacgg
     2461 atgggctccg gccggctctg accggctgat catcggtcac gagtcgctcg gt
//
LOCUS       DASSRL010000102         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_650296, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000102 DASSRL010000000
VERSION     DASSRL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650296"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFL49_02150"
     CDS             complement(<1..636)
                     /locus_tag="VFL49_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET7910807.1"
                     /translation="MNDSTAQPISRVSGGLRFPAHPVMGLAGTSLKHEHLTAILAEGK
                     RAGFFEVHAENYMGAGGPPHHALTRIRRDYPISLHGVCMSIGGSQPLDQAHLGRFKTL
                     VDRYEPALVSEHLAWSTHGTTYFNDLLPLPYTSRTLARVADHIEKVQEAIGRPILLEN
                     PSTYVVFPESTMSETNFIRELVRRTGCGLLLDVNNVFVSATNHGYSALAYLA"
     gene            complement(670..993)
                     /locus_tag="VFL49_02155"
     CDS             complement(670..993)
                     /locus_tag="VFL49_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HET7910808.1"
                     /translation="MSTRSAVSSVVLASAVATAFASYAMAGPLTKAEADAAMAAGKEQ
                     CYGVVLKGQNDCKAGPGTTCQGTSTVDFQGNAWKFVQGGTCENLELPGGRHGSLKPLD
                     RDLPS"
     gene            1187..1720
                     /locus_tag="VFL49_02160"
     CDS             1187..1720
                     /locus_tag="VFL49_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7910809.1"
                     /translation="MEGRDDDELARLMRAAITGDERAYEQLLRQIAGLVRGFARRKVQ
                     SGIDPEDIVQETLLAIHMKRHTWRTDAPVAPWIYAIARHKLIDAFRRRGSRMEIAIDE
                     IAETCAEPGPETIGQREMNRALATLAPGQRSVVVAISVEGHSISETARRLGMSEPAVR
                     VALHRGLAAIAKRFGRN"
     gene            1722..2360
                     /locus_tag="VFL49_02165"
     CDS             1722..2360
                     /locus_tag="VFL49_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET7910810.1"
                     /translation="METNELIRALASDARLRGASSSSVWWGAAGLAIALAAAVFFPTL
                     GPRPDIATAAETPRFLFKFVVTITLAVSAFGLVRMLSRPAETSRRAMLYLVTAPALII
                     MAVIVELFLVPPGTWSTRLVGTNSMVCLTYIPLMGIGPLAIFLVALRHSAPAKPALAG
                     AVAGLLAGGIAATFYAAQCTDDSPLFVATWYTIAITGLALAGAAGGHRFARW"
     gene            2678..>2904
                     /locus_tag="VFL49_02170"
     CDS             2678..>2904
                     /locus_tag="VFL49_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013636466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7910811.1"
                     /translation="MDNLSSHLDHVFGALADPTRRAILARLGEGEASVGELARPFAMA
                     LPSLMKHIRVLEAGGLVESRKSGRVRTCRLKP"
BASE COUNT          522 a          887 c          925 g          570 t
ORIGIN      
        1 ggcgagatat gcaagcgcgg agtaaccgtg attggtagcc gaaacaaaga cattgttgac
       61 gtcgagcaac aggccgcagc ctgtgcgccg gaccagttcc cggatgaagt tggtttcgct
      121 catggtcgat tccgggaaga cgacataggt cgacgggttc tcaagcagga tcgggcggcc
      181 gatcgcctct tgcaccttct cgatgtgatc ggcgacgcgg gcaagtgtcc gcgacgtgta
      241 gggcagagga agcagatcat tgaaataggt cgtgccgtgg gtcgaccacg cgagatgctc
      301 tgaaaccagc gccggctcat agcgatcgac aagtgtcttg aaacgcccga gatgcgcctg
      361 atcgagcggc tgcgacccac cgatcgacat gcagacgccg tgcagcgata ttgggtagtc
      421 acggcggatg cgggtcaaag cgtggtgtgg cgggccgccc gcgcccatat agttctcggc
      481 atgaacctca aaaaagcctg cccgcttgcc ctcggccagg atcgccgtca aatgctcgtg
      541 cttgaggctg gtgcctgcga ggcccatgac gggatgagcc ggaaagcgga ggcctccgga
      601 cacgcgcgaa atcggctgtg ccgtcgaatc attcatcgca gtctccgctt tccggcaagc
      661 tgccttggct taggacggca ggtcgcggtc cagcggcttg agcgaaccgt ggcggccacc
      721 gggcagttcc agattctcgc aggtgccgcc ttggacgaac ttccaggcat tgccctggaa
      781 gtcgacggtc gaggtgcctt ggcaggtcgt tcccggcccc gccttgcagt cgttctgacc
      841 tttgagcacg acgccgtagc attgctcctt gcccgctgcc attgccgcgt ctgcctcggc
      901 tttcgtcagc ggccctgcca tagcgtacga cgcaaaggcg gttgcgacgg cactggcgag
      961 gacgaccgaa ctcacggctg atctcgtaga catgcttctc tccttgtgta gtatttggcc
     1021 gcgcgccatg cctcaggcgc acgatagcca attcgatccg gcgagccgcg tgttacatgc
     1081 gcccaggcgc acgatttcgt gatcggaaga ttgcgtcgtg tgaacaaagt ttgtcgatca
     1141 cgtaacaaaa tgccctgcac gcgcgtaaat ggagaggaag gcaggcttgg aaggcaggga
     1201 cgacgacgaa cttgcccggc tgatgcgagc cgcgatcaca ggtgatgagc gggcctatga
     1261 gcagctcctg cgtcagatag ccggcctcgt acgcggcttc gcccggcgga aggtgcagag
     1321 cggaatcgac ccggaggata tcgtgcagga gacgctcctt gcgatccaca tgaagcgcca
     1381 cacatggcgg acggacgcgc ctgtggcgcc atggatctat gcgattgcgc gccataagct
     1441 gattgatgcc tttcgccgtc gcggcagccg gatggaaatc gcgatcgacg agatcgccga
     1501 gacttgcgcg gagccgggac ctgaaaccat cggccaacga gagatgaacc gcgcccttgc
     1561 gacgctcgca cccggccagc gctcagtggt agtagcgatt tccgtcgagg ggcactcgat
     1621 cagcgagacg gccaggcggc tcggcatgag cgaaccggca gtgcgggtcg cactccatcg
     1681 cggcttggcg gcgatcgcca aacggttcgg gcggaattga catggagacg aatgagctta
     1741 tcagggcctt ggcatccgat gcgcgactgc gcggggcgtc gtcctcgtcg gtatggtggg
     1801 gcgcggccgg tctggcgatc gctcttgcgg ccgccgtctt ctttccgacg ctcggtccga
     1861 ggcccgatat tgcgaccgcc gccgaaacgc cgcgctttct gttcaaattt gtggtcacga
     1921 tcacgcttgc cgtgagcgca ttcggtcttg tccggatgct gtcgcgacca gcggagactt
     1981 cgcgccgggc gatgctgtat ctcgtgaccg cccctgcgct catcatcatg gcggtcattg
     2041 tcgagctttt ccttgtgccg cccggcacct ggtcgaccag gctggtcggc acgaacagca
     2101 tggtgtgcct gacctatatt ccgctgatgg gcattggtcc gctcgccata ttcctggtcg
     2161 cgctacgcca ctccgcaccg gcgaaaccgg cccttgccgg tgcagtcgca gggctgctgg
     2221 ccggcggcat agccgccacc ttctacgccg cgcaatgcac ggacgattcc ccactcttcg
     2281 ttgccacttg gtacacgatt gccattaccg gcctcgctct cgctggagct gccggaggtc
     2341 atcgattcgc ccgctggtag cgacgaaggt ttcactctgg acctggatcg ctgccagccc
     2401 agcccaagct tttgagcttc tgatgccggc tcggccagat ccaacgctta tccccaggtc
     2461 cacaaaatcc gggataggcg cttgcccgcg gcatctcgtg attcgcgctc taccgtgggt
     2521 agcgaccagc gcgtagtcac gcttgcccgc caaccaatag cgcgtccgtt gtgagaggat
     2581 gttcgtctgt cataggcgac gcctcgatca cgtctttagt aagctaaacg gataactgtt
     2641 attgacaact cctcggcgcg cacttatagt tagccgaatg gataacttat catcgcatct
     2701 tgaccatgtg ttcggcgctc ttgcggaccc gaccaggaga gcgatccttg cgcgattggg
     2761 cgagggggag gcgtcggtcg gggagctcgc ccggccattt gccatggcgc tgcccagcct
     2821 gatgaagcac atccgggtgc tcgaggccgg cggcctcgtg gagtcccgga agagcgggcg
     2881 tgttcgcacc tgtcggttga aacc
//
LOCUS       DASSRL010000103         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_929929, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000103 DASSRL010000000
VERSION     DASSRL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929929"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..702)
                     /locus_tag="VFL49_02175"
     CDS             complement(199..702)
                     /locus_tag="VFL49_02175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7910812.1"
                     /translation="MAVAHPKLALRPFLPADATLLREIFRDSIEELTSDDYTEAQQEA
                     WASAADDAAEFRDKLSGQLTLVATLEGSPVGFASLEGKDKIGMLYVHPAATGQGVGAM
                     LVDALEKLAGARGTAKLTVDASDSARGFFEKRGYVAQQRNSISIGDEWLANTTLQKQL
                     AAKREAT"
     gene            complement(702..2069)
                     /gene="cysS"
                     /locus_tag="VFL49_02180"
     CDS             complement(702..2069)
                     /gene="cysS"
                     /locus_tag="VFL49_02180"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7910813.1"
                     /translation="MELKLYDTLTRTKRTFAPIDPARVRMYVCGPTVYDFAHIGNARP
                     VIVFDVLFRLLRHVYGEKHVIYVRNITDVDDKINARAAERGISIREVTEETYRNFKED
                     VAALGCLPPTVEPRATEHIEDMKALIERLVRSGNAYVAEDHVLFHVASMKDYGKLSNR
                     SLDEMVAGARVEVAPFKKDPMDFVLWKPSGPGEPAWPSPAGIKKPGRPGWHIECSAMS
                     WKHLGETFDIHGGGIDLVFPHHENEIAQSRCAFHTPVMANFWMHNGFLQVEGEKMSKS
                     LGNFVTIHDLLKDWPGEVVRFTMLQTHYRQPMNWTLAGLRESQKNLEHWYGLTGDSTP
                     GYLCADAVDALLDDLNTPKAFAALHELRGEAAKGAKPAAASLKASARLIGLLQLPAAQ
                     WTAFRPASATLDETQVVGLIEARDKARKTKNFKEADRIRDQLKAMGIELEDTKDGNTK
                     WKIAR"
     gene            2385..2687
                     /locus_tag="VFL49_02185"
     CDS             2385..2687
                     /locus_tag="VFL49_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910814.1"
                     /translation="MRTVLIGFVVVFCLTPGMSRAEQHMFLVANDADGYGIDRCLASG
                     EKCGAAAANAYCRTQAFTAAATYHKIDQGDLTGATKDASITCQPGTCEVVAIVCLR"
     gene            complement(2697..3095)
                     /locus_tag="VFL49_02190"
     CDS             complement(2697..3095)
                     /locus_tag="VFL49_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7910815.1"
                     /translation="MVKFDQHTDTATLLARILLSAVMWLYGYFKLTGYAGAVTYMSRQ
                     GLPAPAVFAVLAIIIEIGGGLLILFGYQTRLVALGCAIYILVAAFIAHRNFSDGNQLA
                     HFMKNLAITGGYLALMIAGPGRYSLDGRKS"
     gene            3392..4027
                     /gene="maiA"
                     /locus_tag="VFL49_02195"
     CDS             3392..4027
                     /gene="maiA"
                     /locus_tag="VFL49_02195"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HET7910816.1"
                     /translation="MKLYSYFRSSAAYRVRIALNLKGLAYEMIPIHLTKDGGRQHTPD
                     YRSINPQARVPALMLPTGDVLTQSMAIIEYLDETHPEPRLLPADAVGRAKVRAIAQAI
                     ACDIHPLNNLIALQYLRRQFKHEQAEIDAWYHHWIIEGFDALETMLGPGPYAYGATVT
                     LADVCLVPQVANARRLKVPLDKFPKIVAADAACLKLPAFDKARPENQPDAE"
     gene            4098..>4364
                     /locus_tag="VFL49_02200"
     CDS             4098..>4364
                     /locus_tag="VFL49_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910817.1"
                     /translation="MIASKLARVVLWMVGALLSFSVMAVSIRELFRGGLSIFEILAIR
                     SGVALMVLLTILTVHKELRVHALPRRMGLNFFRNGVHYASQFAWA"
BASE COUNT          916 a         1322 c         1198 g          928 t
ORIGIN      
        1 caaaggtaat aattcgagat caaggcaatg gcgatcacaa tcgcgatgac acccatcagg
       61 acatggtgac gtttcatgcg tgtcgttacg tcagtctgta gacgacatag agcgcgacca
      121 ccacggcaac ggcgagcacc ccgtatttaa gaacgatgta atagagccag tgacgtggaa
      181 acggagtgtc gggtctggtc atgtagcctc ccgtttcgca gcaagttgct tctgcagtgt
      241 ggtgttcgca agccactcgt cgccaatgga aatcgagttc cgttgttggg caacatagcc
      301 ccgtttttcg aaaaagccgc gcgcactgtc gctcgcgtcg acggtaagct ttgcggtgcc
      361 ccgcgcacct gcaagctttt ccagggcgtc caccagcatc gcgccaaccc cctgccccgt
      421 tgccgccgga tggacataaa gcatgccgat cttgtccttg ccttcgagcg aagcgaagcc
      481 aaccggcgag ccctccagcg tcgcaaccaa cgtcagttgc ccggaaagct tgtcccgaaa
      541 ttctgcggca tcatcggcgg cggaggccca ggcctcctgc tgtgcctccg tatagtcgtc
      601 gctcgtcagt tcctcgatac tgtcgcgaaa gatctcgcgc aacaacgtcg catccgccgg
      661 caagaacgga cgcagcgcga gcttgggatg tgcaacggcc atcatcgcgc aattttccac
      721 tttgtgttgc cgtctttcgt atcctcaagc tctatcccca ttgccttgag ctggtcgcga
      781 atgcggtcag cctccttgaa atttttcgtc ttacgggctt tgtcgcgcgc ctcgatcaga
      841 ccgaccactt gggtttcgtc gagcgtcgct gatgccggac ggaaggcagt ccactgagcc
      901 gccggcaatt gcagcagacc tatcagccgc gcgcttgcct tcagagaagc ggcggcaggt
      961 tttgcccctt tcgcagcctc gccccgcaac tcatggaggg cggcaaatgc tttgggcgta
     1021 ttgaggtcgt ccagcaatgc gtcaacagca tcggcacaca aataacccgg cgtcgagtcg
     1081 cccgtaagcc cgtaccaatg ttcgaggttc ttctgggact cgcgtaatcc ggcaagcgtc
     1141 caattcatcg gctgccggta atgcgtttgc agcattgtga atcgcacaac ctcacccggc
     1201 caatccttca gcaagtcgtg aatcgtcaca aaattgccga gactcttcga cattttttcg
     1261 ccttcgacct gcagaaatcc gttgtgcatc cagaaattcg ccatcaccgg cgtgtggaaa
     1321 gcgcaacgcg attgcgcaat ctcgttctcg tggtgcggaa aaacgagatc gatgccgccg
     1381 ccgtggatgt cgaaggtctc gcccagatgc ttccatgaca ttgccgagca ttcgatatgc
     1441 caaccggggc ggcccggctt cttgatgccg gccggcgatg gccatgccgg ctcgccagga
     1501 cctgaaggct tccacaagac gaaatccatc gggtctttct tgaacggcgc gacttccaca
     1561 cgggcgcccg cgaccatttc atcgagtgag cggttggaaa gtttgccgta gtccttcatc
     1621 gacgcgacat ggaagaggac gtgatcttcg gcgacatagg catttccgga cctcaccagg
     1681 cgctcgatca atgccttcat gtcttcgata tgctcggtcg cgcgcggctc gacagtcggc
     1741 ggcagacagc cgagtgccgc cacatcctcc ttgaaattcc tgtaggtttc ttcggttacc
     1801 tcgcggatcg aaattccacg ctcggcggca cgcgcattga tcttgtcatc gacgtcggtg
     1861 atgttgcgca catagatgac gtgcttttcg ccgtagacat ggcgcagcaa gcgaaacagc
     1921 acgtcgaaga cgatgacggg gcgcgcattg ccgatatggg cgaaatcgta gaccgtcggc
     1981 ccgcaaacat acattcgcac gcgcgcaggg tcgatcggag caaacgtccg cttcgtccgc
     2041 gtcagcgtat cgtaaagctt gagttccatg gcaaatgaca cccatcggcc gcgctggccg
     2101 ggcgtcgtgc tatctcaatc ttgagaaaag acggccgcag ccagcgtggc gcgctagcga
     2161 ataatctccg ggcaaatgcc gcagatatgg ttaaggccgt tcatggcggc acaatgattt
     2221 gtagcgcgcg cgccgtcaag cacccgcctc aacccgtgaa aaatacggga aaaaatcgat
     2281 gagcgggcac cgcgctcggt taagtgctga ttaacgattt tagccgcatt ttcgcggtcg
     2341 atcgcggcac agtctctgcg ccaagacatt cccgagcgcc attcatgcgg acagttctga
     2401 tcggcttcgt cgtcgttttt tgcctcaccc ccggcatgtc gcgtgccgag cagcatatgt
     2461 ttctcgtcgc caacgatgcc gatggctacg gcatcgatcg ctgtctggcg tcaggagaaa
     2521 agtgcggcgc ggcagcggca aacgcctatt gcagaacaca ggcgttcaca gccgccgcga
     2581 cctaccacaa gatcgatcag ggcgatctca cgggtgcaac gaaagacgct tccatcacct
     2641 gtcagccagg cacgtgcgaa gtcgttgcga ttgtttgcct acgttgagcg tcgacgttac
     2701 gacttgcggc cgtcaagcga atagcgcccc ggcccggcaa tcatcaatgc caagtagccg
     2761 cctgtgatcg ccagattttt catgaaatgc gcgagctgat tgccgtcgga gaaattgcgg
     2821 tgcgctataa atgcagcgac caaaatgtag attgcgcaac ccaaagccac cagccgcgtc
     2881 tgatatccga ataggatcag cagacctcca ccaatttcga tgatgatggc taggacggca
     2941 aatactgcgg gagctggcaa gccttgtctg gacatatacg tcaccgcgcc cgcgtagccg
     3001 gtcaacttga aatacccgta tagccacatc accgctgaaa gtaagattcg ggcaagaagc
     3061 gttgctgtat ctgtatgttg atcgaatttc accatggccg tcccccttgt tcccttccca
     3121 tcattgacat gccgcacccc ccgaggcagt gacgcaagca gaatagcatg ctcgacggac
     3181 cgccgcggct gaatttgcat cctcctgtgg gacgcttccc gagttcgatc cgctcgcgtg
     3241 cgaccgactc cgatgcagag cgagaatctt ttaagatcca atctattagg gaaatgcggt
     3301 ccgaacccga aagccgattg aaatcgaggg gccttgtcgc caactggtcc ggtcctcgct
     3361 agattgggcc ggtccacgct ggagcagtct cgtgaaactc tattcgtatt tccgttcgtc
     3421 cgccgcctac cgcgtccgca tcgcccttaa tctcaaaggg ttggcgtatg agatgatccc
     3481 gatccacctc accaaagacg gcggacgaca gcacacaccc gactatcgca gcatcaatcc
     3541 acaggccagg gtgccggcac tgatgctgcc caccggcgac gtgctgaccc agtcgatggc
     3601 gatcattgaa tatctggatg aaacgcatcc cgagccccgg ttgctgcctg cagacgctgt
     3661 cggccgcgcg aaggtgcgtg cgatcgctca agccattgcc tgcgacattc acccgctgaa
     3721 caatttgatt gcgctccagt atctcaggcg tcagttcaag cacgagcaag ccgagatcga
     3781 tgcctggtac catcactgga tcattgaggg atttgacgcg ctcgaaacca tgctcgggcc
     3841 cggcccgtac gcgtacggcg cgaccgtcac gctcgccgac gtctgcctgg tcccacaggt
     3901 tgcaaacgct cgtcggctga aagttccact cgacaaattc ccaaagattg tcgccgctga
     3961 tgctgcctgt ctgaagcttc cggcgttcga caaggcacga ccggagaacc agccggatgc
     4021 ggagtgacgt caatttggct atctgctaga ccttccgccc cgcacggact ggtcccgtca
     4081 tatcagcaga taaacccgtg atcgcaagca agctcgcacg cgtcgtcctt tggatggtcg
     4141 gcgcactgct gtcattttcg gtgatggcgg tatccattcg cgaattattt cgcggcgggc
     4201 tgagcatttt tgagatcctg gcgatccgca gcggcgtagc gctcatggtt ttgctgacca
     4261 tcctcaccgt gcacaaagag cttcgcgtgc atgctcttcc gcgccgcatg ggactcaatt
     4321 ttttccgtaa tggagttcat tatgcgtcgc aattcgcatg ggca
//
LOCUS       DASSRL010000104         4767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1682517, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000104 DASSRL010000000
VERSION     DASSRL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682517"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFL49_02205"
     CDS             complement(<1..510)
                     /locus_tag="VFL49_02205"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7910818.1"
                     /translation="MAAAQPPQAQSNLGGGFIEYIFGNGAPPPRRGSQQWYGPPPEQN
                     DYRGYGNAAVDPRMDQQRYDERMDPRYLRQEVAYGGKEAPGTIVIDTPNHFLYLVEGE
                     GRAIRYGIGVGRPGFTWSGVHAISGKKEWPDWVPPDEMLRRQPYLPHFVAGGPNNPLG
                     ARALYLGSTL"
     gene            811..1434
                     /locus_tag="VFL49_02210"
     CDS             811..1434
                     /locus_tag="VFL49_02210"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HET7910819.1"
                     /translation="MQRKAFRVEEMIGARRASNGAQRLPANEHAINEPPPAAETNLTA
                     GLELSTLIDVIARNRRALMALVNDGKDRRMARAAGELAAAVESMETATQKVLTSVEAI
                     DDCARVLVSALTDDYHHGLAQDIQDHVVRVYEACNFQDLAGQRIGKVISILVMMEEQL
                     AVMIENCGSVVGDGTAMPKRVNDLLNGPRLDGDGGHASQQDIDRIFA"
     gene            complement(1431..2621)
                     /locus_tag="VFL49_02215"
     CDS             complement(1431..2621)
                     /locus_tag="VFL49_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7910820.1"
                     /translation="MISPRQFVSGPWRAVLVLGITQILTWGTIFYTPVLIVPLIAAER
                     GWSITFAMGGFSIGLLVAGLVAPYVGRSIDRLGGHVVMALGSLTGAVGLFLIVHVAQP
                     MAYYAVWMLLGIAMAANLYDAAFSTLGRIFGVGARRPITALTLAGGFASTVSWPMTHV
                     LLEHMDWRGTYLIYAALLAGVAAPLHALALPRTRYQASATAPDSENAPVKVLPAYGLP
                     FILVASAFAAYAFVPSGLSAHLLAIFAREGIGAATVVWIGALFGPAQVGARLIEFAFG
                     RNLHPLWIARFALATFLCAFLMLVLLGVSPGTAAAFALMFGGANGLITITRGAVPLAL
                     FGASGYGRLMGRLAAPFLLVQAAAPFVMAFMIERTSDLGALSLAAAFASVALICFILI
                     RRPA"
     gene            complement(2692..3264)
                     /locus_tag="VFL49_02220"
     CDS             complement(2692..3264)
                     /locus_tag="VFL49_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910821.1"
                     /translation="MIQPARPRFSCIAVFALLALGLSGCATGPQPEQPAAPAAVPTLP
                     PAFPPQDIVGRWGLAAYHKEEDRARTEAAAARGCNQPYVITLGPTGGVMMHLADQATP
                     TELSLKGAPGGKTFIGPAEDPPGSAQDREVVLFNGRVLILRWMDSEVQGRYGTMVYVR
                     CGAEGARKPAAKPRAPAKPKTAPPPPPPAR"
     gene            3492..3788
                     /gene="groES"
                     /locus_tag="VFL49_02225"
     CDS             3492..3788
                     /gene="groES"
                     /locus_tag="VFL49_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7910822.1"
                     /translation="MAKTKFRPLHDRVVVRRIDAEEKTKGGIIIPDTAKEKPQEGEVI
                     AVGPGGRDENGKLIPIDLKPGNRVLFGKWSGTEVKLDGEELLIMKESDIMGVIA"
     gene            3821..>4767
                     /gene="groEL"
                     /locus_tag="VFL49_02230"
     CDS             3821..>4767
                     /gene="groEL"
                     /locus_tag="VFL49_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET7910823.1"
                     /translation="MAAKDVKFSGDARERMLRGVDILANAVKVTLGPKGRNVVLDKSF
                     GAPRITKDGVTVAKEIELDDKFENMGAQMVREVAQKTNDLAGDGTTTATVLAQSIVRE
                     GAKSVAAGMNPMDLKRGIDIAVTAVVKDIEKRAKKVGSTAEVAQVGTISSNGDATIGK
                     MIAQAMQKVGNEGVITVEEAKSLETDVEIVEGMQFDRGYISPYFITNAEKMLAELEDA
                     YILLHEKKLSGLQSMLPVLEAVVQSGKPLVIIAEDVEGEAIATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAVLTSGQLISEDLGIKLENVTVA"
BASE COUNT         1045 a         1461 c         1494 g          767 t
ORIGIN      
        1 gagtgtcgaa ccgagataga gcgcgcgggc ccccagggga ttgttcgggc cgcccgccac
       61 gaaatgcggc aggtagggct ggcgcctgag catttcatcg ggcggcaccc agtccggcca
      121 ttccttcttt cctgaaatcg catgcacgcc tgaccaggtg aagccggggc ggccgacacc
      181 gatgccgtaa cggatagccc tgccctcacc ttccaccaga taaagaaaat ggttcggcgt
      241 atcgatgacg atggtgccgg gcgcttcctt gccgccatac gcgacttcct gccggaggta
      301 gcgcggatcc atgcgctcgt cgtaccgctg ctgatccatg cgtggatcga cggccgcatt
      361 gccataacct ctgtaatcgt tctgctccgg cggcggcccg taccactgtt gcgagccgcg
      421 gcggggcggc ggcgcaccat taccgaatat gtattcgata aacccgccac cgagattcga
      481 ctgcgcctgc ggcggctgcg ccgccgccat caccacgggc acttgatcgt cagcatcggg
      541 cggggtcagg gacaacggct ccgccgacgc aaaactcgat agcaagactg ctgataacac
      601 gacgaaaccc gaacgcaaca tcgaaccacc ctgcactgtc actgtgtcag cgcagtaaaa
      661 cgtcaaacgc ccaccgattg gtaaattgcc gtcaaccacg attccctatg gtaaacgagc
      721 ggtttccgcc gccgcctgcc acccggaaat cgcaacgcgg tattaatgcg caacctctaa
      781 gaagcccggt gtcctcgccg ggaatcaagc gtgcagcgca aagcgtttcg tgtggaagag
      841 atgatcggag cgcggcgcgc aagcaacggc gcacagcgac ttccggcaaa cgaacacgcc
      901 atcaatgaac cccctcccgc agccgagacc aaccttacgg caggactgga gctttcgacg
      961 ctcatcgacg tcattgcgcg caacaggcgc gcgctgatgg cgctggtcaa tgacggcaag
     1021 gaccggcgaa tggcacgagc ggcaggcgag ctggcggctg cagtcgaaag catggaaacg
     1081 gcgacacaga aggtcttgac gtcggttgaa gcgatcgatg attgcgcgcg cgtgctcgtt
     1141 tccgcattga ccgatgacta ccatcacggt ctcgcccagg acatccagga tcatgtcgtg
     1201 cgtgtctatg aagcctgcaa tttccaggat ctggctggtc agcgcattgg aaaagtcata
     1261 tcgattctgg tgatgatgga agagcagctc gctgtcatga ttgaaaattg cggcagcgtc
     1321 gttggcgacg gaacggccat gccgaagcgg gtcaacgatc tcctgaatgg accaaggctc
     1381 gacggcgacg gcggtcacgc cagccagcag gacatcgatc ggatattcgc ctaggccggc
     1441 cggcgaatca gaataaagca gatcagcgcg accgaggcga aagccgctgc aagactgagc
     1501 gcgccaaggt ccgatgtgcg ctctatcatg aacgccatga caaaaggcgc cgctgcctgg
     1561 accagcagga atggcgcggc cagtcgcccc atcagccgcc cgtatccgga ggcgccgaac
     1621 agcgccagcg gcactgcacc gcgcgtgatg gtgatcaggc cgttggcgcc gccgaacatc
     1681 agcgcgaagg ccgccgctgt gccgggcgag acgcccagca gcaccagcat caggaaggcg
     1741 cagagaaagg tggcgagcgc gaagcgcgcg atccacagag ggtgcaaatt gcggccaaag
     1801 gcgaattcga tgagccgcgc gccaacctgg gcggggccga acaaggcgcc gatccacacc
     1861 acggttgccg cgccgatacc ctcacgcgca aatatggcca gcaagtgtgc cgatagcccg
     1921 gacgggacga aggcataggc tgcaaacgcc gaagccacca gaatgaatgg caatccgtag
     1981 gccggtagca ctttcaccgg cgcattttcc gaatctggcg cagttgcgct ggcttgatag
     2041 cgcgttcgcg gcagcgccaa agcgtgcagc ggagccgcga caccggccag caatgccgca
     2101 tagatcaaat atgtcccgcg ccagtccatg tgctcgagca ggacatgcgt catcggccag
     2161 ctgaccgtgg aggcgaaccc gccggcgagc gtcagcgcgg taatcggccg gcgcgcgccg
     2221 acgccgaaga tgcggccaag cgtggaaaac gcggcgtcat aaaggtttgc ggccatggcg
     2281 atgccaagca acatccagac ggcgtaatag gccatcggct gcgccacgtg gacgataagg
     2341 aatagaccga ccgcaccggt cagcgaaccg agtgccatga cgacatgacc gccgagccgg
     2401 tcgatcgatc ggccgacgta aggggcaacc agcccggcga cgagcagacc gattgaaaat
     2461 ccgcccatcg cgaatgtgat tgaccagccg cgctcggccg cgatcaacgg cacgatcagt
     2521 acgggcgtgt agaaaatcgt tccccacgtc agaatctggg tgatgccgag aaccagcacg
     2581 gcgcgccagg gtccgctgac gaattgacgg ggagagatca tgaggggcgc accaaagcag
     2641 gcatgcgcaa ctttgccacg cgactacggt gagtggtacc aaatggctgt gttatcgcgc
     2701 cggtggcggc ggcggtggtg ccgttttggg cttggcgggt gcgcgcggct tggccgccgg
     2761 cttccttgcg ccctccgcac cgcagcggac atagaccatc gtgccgtagc gaccctgcac
     2821 ttcggaatcc atccagcgca ggatcaacac gcggccattg aacaagacga cctcgcggtc
     2881 ctgcgcgctg cccggtggat cttcggcggg cccgatgaat gtttttccgc ccggcgcgcc
     2941 cttcagactc agttcggtcg gcgtcgcctg gtccgcgagg tgcatcatga cgccccccgt
     3001 cgggccgagc gtaatgacat agggttgatt gcagccccgg gccgcagccg cctcggtgcg
     3061 cgcgcggtcc tcctccttgt gataggcagc aagcccccat cggccgacaa tgtcctgcgg
     3121 cgggaaggcc ggcggcaatg tcgggacagc ggccggagca gccggttgct cgggctgcgg
     3181 cccagtggcg cagccggaaa gtccgagggc caacagagcg aaaactgcaa tgcacgagaa
     3241 cctggggcgg gcgggctgaa tcattccggc tcctcggaag cagatcgcga ctttggaggt
     3301 cattcagcgc aagcgcaagc acacattcgc ccatcaggat ttgcgccttt ttgatccgct
     3361 aagcttgaca ctccggctcg aaggcaatat ttcccaagca gtgtttggca ctcacagctt
     3421 atgagtgcta aaccactgat ataccgatac tttctgttca tgagacgcgc attagcgcca
     3481 cgaggaagcc catggctaaa accaaatttc gtcccctcca cgatcgcgtc gtcgtacgca
     3541 gaatcgacgc cgaagagaaa accaaaggcg gcatcatcat cccggatacc gccaaggaga
     3601 agccgcagga aggcgaagtc attgctgttg ggcccggtgg ccgcgacgag aatggcaagc
     3661 tcattcccat cgatctcaaa cccggcaacc gcgtgctgtt cggcaagtgg tcgggcaccg
     3721 aggtcaagct cgacggcgag gagctcctga tcatgaagga atccgacatt atgggcgtga
     3781 ttgcctgaca ccacaccgtt agttcaagga gtagtttgaa atggctgcca aggacgtcaa
     3841 attttccggc gatgcccgcg aacggatgct gcgcggcgtc gatatcctag ccaacgccgt
     3901 caaggtcacg ctcggcccga aaggccgcaa tgtcgtgctc gataaatcgt tcggcgcgcc
     3961 gcgcatcacc aaggacggtg tgaccgtcgc caaggagatc gagctcgacg acaagttcga
     4021 gaacatgggc gcgcagatgg tgcgcgaagt cgcccagaag accaacgatc tcgccggcga
     4081 cggcaccacc accgcgaccg tgctcgcgca gtcgatcgtc agggaaggcg ccaagtcggt
     4141 tgccgccgga atgaacccga tggatctcaa gcgcggcatc gatatcgcgg tgaccgccgt
     4201 ggtcaaagat atcgagaagc gcgcgaagaa agtcggctcc accgcggaag tcgcgcaggt
     4261 cggcacgatc tcgtcgaacg gcgatgcgac gatcggcaag atgatcgcgc aggctatgca
     4321 gaaggtcggc aacgagggcg ttatcaccgt cgaggaagcg aagtcccttg aaacggatgt
     4381 cgagatcgtc gagggaatgc agttcgatcg cgggtacatc tcgccttact tcatcaccaa
     4441 tgccgaaaag atgctcgccg agctcgagga tgcctacatc ctcttgcatg aaaaaaagct
     4501 ctccggcctg cagtcgatgc ttccggtgct ggaagcggtg gtgcagtcgg gcaagcccct
     4561 ggtcattatc gccgaagacg tggaaggcga agcgattgcg accctggtgg tcaacaagct
     4621 gcgcggaggc ctgaaggtcg ccgcagtgaa agctcccggc ttcggcgatc gccgcaaggc
     4681 gatgcttgag gacatcgcgg ttctcaccag cggccagctg atctccgaag atctcggcat
     4741 caagctggag aacgtcacgg tcgccat
//
LOCUS       DASSRL010000105         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_90230, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000105 DASSRL010000000
VERSION     DASSRL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90230"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="cimA"
                     /locus_tag="VFL49_02235"
     CDS             complement(<1..1019)
                     /gene="cimA"
                     /locus_tag="VFL49_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET7910824.1"
                     /translation="MAKPRSRLSRKPSAKAALKAPPAPTSALLEIYDTTLRDGAQAED
                     VSFSADDKVMVAQRLDELGVQFIEGGWPGANPRDIEFFRMIKETPLKQATVVAFGSTR
                     KASNPVQKDSNIQALLQAETKVITLFGKSWSLHVTDALGIALQTNLDLIADSVAYLRS
                     KHRRVFYDAEHFFDGYKTNPEYALETIRRAVEAGAERVILCDTNGGTMPWEIKKICGV
                     VQKECQVPLGIHAHNDCEMAVANSLVAIEMGVVQVQGTINGIGERCGNANLCSIIPNL
                     QLKMKRPALGERLTHLKEVSGFVTEIANLMPNKHQPYVGDSAFAHKGGVHIHAVLKNP
                     ATYEHV"
     gene            complement(1075..>2068)
                     /locus_tag="VFL49_02240"
     CDS             complement(1075..>2068)
                     /locus_tag="VFL49_02240"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7910825.1"
                     /translation="LLAMELRGRGVDARSFTGRQVGIMTDNAHTKARITRVTAERIRD
                     ALAKGVVPVVAGFQGINEQSDVTTLGRGGSDLTAVALAGALKADRCVIFTDVDGVYTT
                     DPNIVPTARRIEKIAYEEMLEMASLGAKVLQSRSVEFAAKYNVPLEVNSSFKEGKGTL
                     VKREDADMEAVAVSGITGDRNQAKITLVGVPDKPGIAARLLGPVAKANILVDMIIQNV
                     SHESLTDFSFTIPRVDVKKAMPIIHETAKDIDAKSVSVTEAIAKVSLVGVGMRSHSGV
                     AAKMFDVLSREGVNIMMISTSEIKISCVIDEKYMELAMRALHAAFGLDRPPAFK"
BASE COUNT          398 a          627 c          588 g          455 t
ORIGIN      
        1 acatgctcgt acgtggcagg atttttcaac acggcatgga tgtgcacgcc gcccttatgc
       61 gcaaaggcgg aatcgccgac atacggctgg tgcttattgg gcatcagatt ggcgatttcc
      121 gtgacgaacc ccgacacttc cttcaagtgg gtgagccgct cgcccagtgc agggcgtttc
      181 atcttcaact gaagattcgg aatgatcgaa cacaggttcg cgttgccgca ccgctcaccg
      241 atgccgttga tggtgccctg tacttgcacc actcccatct cgatggcgac cagcgaattg
      301 gcgaccgcca tttcacaatc gttatgcgcg tggatgccga gcgggacctg gcattctttt
      361 tggaccactc cacagatctt cttgatttcc cagggcatcg tgccgccgtt cgtatcgcaa
      421 aggatcaccc gctccgcgcc tgcctccacc gctcgccgga tcgtctcaag cgcatactcg
      481 gggtttgtct tatagccgtc gaaaaagtgc tcggcatcgt agaacacccg gcggtgtttc
      541 gagcgcagat aagccactga gtcggcgata aggtcgagat tggtctgcag cgcgattccc
      601 agggcatccg tgacgtgcaa cgaccagctc tttccgaaca acgtgatgac cttggtctcc
      661 gcctggagca gggcctgaat attcgagtct ttctggacgg ggttgctggc ttttctcgtg
      721 gagccgaatg ccacgacagt cgcctgtttc aagggcgttt ccttgatcat gcggaaaaac
      781 tcgatatctc tcggattcgc gcccggccag ccgccctcga tgaactggac ccccaactcg
      841 tccaaccgtt gggcaaccat caccttgtca tcggccgaaa aactgacgtc ttccgcctgg
      901 gcgccgtcgc gaagggtcgt atcgtagatt tcgaggaggg ccgatgtcgg ggcaggcggc
      961 gctttcaacg ccgccttggc cgagggtttg cgactgaggc gtgaccgcgg tttagccatg
     1021 gccgaaaggg taacaggagg aggccgagcc tgtcgagggt caaatcaccg ggcctcattt
     1081 gaaggccggt ggccggtcca acccaaaggc ggcatggagg gcgcgcatgg ccagctccat
     1141 gtacttttcg tcgatgacgc aggagatttt gatctcggat gtgctgatca tcatgatatt
     1201 gacgccttca cgggaaagga cgtcgaacat cttggcggcg actcccgaat gcgaacgcat
     1261 accgacgccg accagcgaca ctttggcaat ggcctcggtc acggagaccg acttggcgtc
     1321 gatgtctttc gccgtctcgt gaatgatcgg catcgctttt ttgacgtcga ccctcggaat
     1381 cgtaaacgag aagtcggtca aggattcatg gctgacattc tgaatgatca tatcgacgag
     1441 aatattcgct ttcgcgacag ggcccaacaa gcgcgccgca atgcccggct tgtccggaac
     1501 gccgacaagc gtgatcttcg cctgattgcg gtcgccggta atgccggaaa cggcgacggc
     1561 ctccatgtct gcgtcttcgc gtttcacgag cgtccccttt ccttccttga aactggaatt
     1621 gacctccagc ggcacattgt acttcgcggc gaattcgact gagcggctct gcagcacctt
     1681 ggcgccaaga ctggccatct caagcatctc ttcgtacgca atcttttcga tccgcctggc
     1741 cgtcgggacg atattgggat cggtcgtata caccccgtcc acatcggtga agatgacgca
     1801 ccgatcggct ttcaaggccc cggccaaggc caccgccgtg agatccgatc cgccacgccc
     1861 caacgtcgtg acgtccgatt gttcgttgat tccttgaaag ccagccacga ccgggacgac
     1921 gcctttcgcg agcgcgtcgc ggatccgttc agccgttacc cgggtgattc gcgctttggt
     1981 atgggcgttg tccgtcatga tgccgacctg ccgcccggtg aacgatcgtg catccactcc
     2041 gcgtccgcgc aattccatgg ccaacagt
//
LOCUS       DASSRL010000106         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1297449, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000106 DASSRL010000000
VERSION     DASSRL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297449"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFL49_02245"
     CDS             <1..249
                     /locus_tag="VFL49_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET7910826.1"
                     /translation="FAGRGFKRQVPIGPHITDFVSFPLKCVIDLVPAGETDDAARTRA
                     ERRTYLIERGYKVCEVRGAEVGKDAGRVLDRLHGLVGQ"
     gene            complement(250..1023)
                     /locus_tag="VFL49_02250"
     CDS             complement(250..1023)
                     /locus_tag="VFL49_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7910827.1"
                     /translation="MPAFALARRLRAGETIYAAWCMIGSQLVAETIAREGFSAVVLDA
                     QHGLWDIASLITGIGAVSHAEATPVVRIPLNDLALVSRALDFGAEAIIAPMINSADDA
                     QRFATAAKYPPSGERSWGPLRAMALQAPRTAPADYLREANEGTLTFAMIETATALGNA
                     DKIAATPGIDGLFVGPYDLATALSGGKAQDTQAPQVEQAIDRICAAAKTAGKISGIYC
                     RDAEGAVAMAKRGFRFVVAGNDHTTLRSATVAQLKVLKG"
     gene            complement(1025..1342)
                     /locus_tag="VFL49_02255"
     CDS             complement(1025..1342)
                     /locus_tag="VFL49_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HET7910828.1"
                     /translation="MTTFDKREEGFEKQFAHDEELRFKAMARRNKMLGLWAAGVLGKS
                     GTDAEAYAKEVVLADFEEAGDNDVLHKVLRDLQPKGFTEQQIRAQMTELLAQAVAQIK
                     KSG"
     gene            1621..>2377
                     /gene="purC"
                     /locus_tag="VFL49_02260"
     CDS             1621..>2377
                     /gene="purC"
                     /locus_tag="VFL49_02260"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7910829.1"
                     /translation="MDFHKPRYTPMSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATA
                     FNAKKHEVIEGKGVLNNRISEYVFQHLNDIGVPTHFIRRLNMREQLIREVEIIPLEIV
                     VRNVAAGSLAQRLGIEEGTQLPRSIIEFYYKNDKLNDPMVSEEHITAFGWASPQEIDD
                     IMALAIRVNDFLSGLFLGVGIRLVDFKMETGRLWENDVMRIVVADEISPDSCRLWDIK
                     TNDKLDKDRFRRDLGGLVEAYTEVAKRLGIMSEG"
BASE COUNT          449 a          734 c          736 g          458 t
ORIGIN      
        1 ttcgccggcc gcggcttcaa gcgccaggtt ccgatcggcc cgcatatcac cgacttcgtg
       61 tcgtttccgc tgaagtgtgt gatcgacctg gtcccagcgg gtgaaaccga cgacgcagcg
      121 cggacgcggg ccgaaaggcg cacctacctg atagagcgtg gctacaaagt gtgcgaagta
      181 cggggcgccg aggtgggaaa agacgcaggc agagtgctcg atcggctcca cgggctggtc
      241 gggcagtagt taccccttca acaccttcag ctgcgcaacc gtggccgagc gcagggtcgt
      301 gtggtcgttg ccggcgacga cgaaacggaa gccgcgcttc gccatggcta cggcaccttc
      361 ggcgtcgcgg caataaatgc cggagatttt tccggcggtt ttagcagcgg cgcaaatcct
      421 gtcgattgcc tgctcgacct gcggtgcctg ggtgtcctgc gcttttccgc cgctcagcgc
      481 cgtggcgaga tcgtaagggc cgacgaacag tccatcgatg cccggcgttg ccgcaatctt
      541 gtcggcattt ccgagcgcgg ttgcagtctc gatcattgca aaagtgagag tgccctcatt
      601 ggcttcgcgc aggtagtccg ccggcgcggt gcgtggcgcc tgcagcgcca tcgcgcgcag
      661 cgggccccag ctgcgctccc cggagggcgg atatttcgcc gctgtagcga accgttgggc
      721 gtcatcggcg ctattgatca tcggcgcaat gatggcttcc gcgccgaaat ccagcgcgcg
      781 cgagacaagt gctagatcgt tgagcggtat gcggacgacc ggtgtcgctt ccgcgtggct
      841 gacggcgccg atgccggtga tgagtgaggc gatgtcccag agcccgtgct gggcatccag
      901 tacgaccgca gagaacccct cgcgcgcgat ggtttccgcg accagctgcg agccgatcat
      961 gcaccaagct gcgtagatcg tctcccccgc ccgcaggcgc cgcgcgaggg caaaagccgg
     1021 cacgtcagcc gctctttttg atctgcgcga cggcctgggc aagcaattcg gtcatttggg
     1081 cgcggatttg ctgctccgtg aagcccttcg gctgcaggtc cctcagaacc ttgtgcaaga
     1141 cgtcgttgtc gccggcctct tcaaagtctg ccagcacgac ctccttggcg taggcttccg
     1201 catccgtgcc gcttttgccg aggacgccgg cggcccagag cccgagcatc ttgttgcggc
     1261 gggccatcgc cttgaagcgg agttcctcgt catgcgcgaa ctgcttttcg aagccttcct
     1321 cgcgcttgtc gaatgtcgtc atgccgcccc tcgtactgca tcggtaaaat tgcatgcaca
     1381 tgcaagtgag ttcggccttg catatcgtcc gcagaccgtc aaggcaacat cgggccgcgt
     1441 ttttgcggcg aaaatacgga gtggaaaacg taggcgcggc gtgattgtgc tgctccgacc
     1501 gatcggctag gttgcccttg tgcccggacc gctggccatg accagattgg ccagaattcc
     1561 agtccggtcc cgcagccgcg gatcttgcaa agtcgcacaa caaggagcca cggcaacccg
     1621 atggactttc acaaaccacg gtacacgccc atgagccgcc gtcgccgtat ttacgagggc
     1681 aaggcgaagg tcctctatga aggccccgag ccgggaacgc tgattcagca cttcaaggac
     1741 gacgcgaccg ccttcaacgc caaaaagcat gaggtgattg aaggcaaagg cgtgcttaac
     1801 aaccgaattt cggaatatgt gttccagcac ctcaacgata tcggggtgcc aacacacttc
     1861 attcgccgcc tcaatatgcg cgagcagctg attcgcgagg tggaaattat tccgcttgag
     1921 atcgtggtac gaaatgtcgc ggcgggctcg ctggcgcagc gccttggcat cgaagagggc
     1981 acgcaactgc cccgttcgat catcgagttc tactacaaga acgataagct caacgatccg
     2041 atggtctcgg aagagcacat caccgccttc ggctgggcgt cgccgcagga gatcgacgac
     2101 atcatggcgc tggccattcg tgtcaacgac ttcctgtccg gacttttcct cggcgtcggt
     2161 atccgactgg tcgacttcaa gatggaaacc ggccggctct gggaaaacga cgtgatgcgc
     2221 atcgtcgtgg ccgacgagat ttcccccgac agttgccggt tgtgggacat caagaccaac
     2281 gacaagctcg acaaggaccg tttccgccgc gatctcggcg gcctcgtcga ggcctacacc
     2341 gaggtcgcca agcgtctcgg catcatgagt gaaggcg
//
LOCUS       DASSRL010000107         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_772449, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000107 DASSRL010000000
VERSION     DASSRL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772449"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFL49_02265"
     CDS             <1..348
                     /locus_tag="VFL49_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7910830.1"
                     /translation="GVKTTPYNLLTAARSFGASEWQIFKSVVIPSTVPFILTGLRLAV
                     GRALIGVMVGDLYAATAGIGFMITVAGATFQTDKVFVGVLIFAITGMVATEMIDRAER
                     KFDRWRPKVGADH"
     gene            complement(534..1094)
                     /gene="pal"
                     /locus_tag="VFL49_02270"
     CDS             complement(534..1094)
                     /gene="pal"
                     /locus_tag="VFL49_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET7910831.1"
                     /translation="MNNNHYATVAVCLLLAGGLAACSSTDASKSAAAPATAQARAATK
                     PKPQARAQSQSSLDALRHGDSAATPKDGALKAVFFEFDRYDLSSDARATLKAAADWLR
                     NNPAVKVEVEGHCDDRGTNEYNLALGAKRAQAAKDYLVTLGVGAARLSTTSYGEEVPV
                     CREHSEACWQKNRRDRFVALGAKPGV"
     gene            complement(1154..1828)
                     /locus_tag="VFL49_02275"
     CDS             complement(1154..1828)
                     /locus_tag="VFL49_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7910832.1"
                     /translation="MPGLDWHFILSWLKIVVLDLTLAGDNAVVIGMAVRALPARQQRI
                     GVCLGALGAVIARVALTFAAARFLFFPYVQLAGGLVLVWIACKLLRQDRRSTPAMRQG
                     ATLPQAIRIIIAADLVMSTDNILAIAGASGGSFFLVLFGLGLSIPIVVAGAALIAVLM
                     SRFRWPTYIGAAILGEVAGKMILEDDFVEVTLGEASKTLEWEIRIGLALVLVVAGFLL
                     ARRPAK"
     gene            complement(1945..>2261)
                     /locus_tag="VFL49_02280"
     CDS             complement(1945..>2261)
                     /locus_tag="VFL49_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910833.1"
                     /translation="HREEVIRFLRALLKARRWIDEHRQEAAEFLAEELDMKPALARRG
                     LDYYVDQRAWDPLLRIDLDGLKTVIAVYAEQADLKGPLPDPAKYVDSSYLEQALGELG
                     WK"
BASE COUNT          457 a          711 c          653 g          440 t
ORIGIN      
        1 ggcgtcaaaa ccacgcctta taatctgctc accgccgcga gaagttttgg cgcctcggaa
       61 tggcagatct ttaaaagcgt ggtcattcca tcgacggtcc cattcatact taccgggctg
      121 cgattggccg tcgggcgcgc gttgatcggc gtcatggtcg gagatctcta tgccgccacc
      181 gccggcatcg gctttatgat cacggtggca ggcgcgactt ttcagaccga taaagtcttc
      241 gtcggcgtcc tcatctttgc catcaccggc atggttgcca cggaaatgat cgacagagcc
      301 gagcgaaaat tcgacagatg gcggccgaag gtcggggccg accattagta attcatcgag
      361 cgcttcgact catttcagca ccgagaacaa actggccagc ctaacccgta agagttgggc
      421 tggccactat ttagaccacg agaaagccct atctgcagga accgcgcggc tcaatcgctt
      481 agatcattcg cacgtacgca gggcatggtc gaaacgcatc gcgcgatcag aaattagacc
      541 ccgggcttgg cgcccaaggc gacgaatcgg tcgcgacgat ttttctgcca gcacgcttcg
      601 ctgtgttctc ggcatacggg cacttcttcg ccgtagctcg tcgtcgacag acgtgcggcg
      661 ccgacgccca aggtcacgag atagtctttc gccgcttgcg cgcgtttcgc gcccaatgcc
      721 agattgtact cgttggtgcc gcggtcgtcg cagtggcctt cgacttcaac cttcaccgcg
      781 ggattgtttc gcagccaatc ggccgccgct ttgagagttg cgcgcgcatc actggacaag
      841 tcataacgat caaactcgaa gaagaccgcc ttgagcgcgc catccttcgg tgtggccgcg
      901 gaatcgccgt ggcgcagcgc gtccagactg ctttgcgact gagcgcgcgc ttgcggcttc
      961 ggtttagtcg cggctctggc ttgcgctgtc gccggcgcgg ccgcggactt tgatgcgtcc
     1021 gtgctcgagc atgccgccaa tccgcctgcc aaaagcaggc aaaccgcaac ggtcgcgtaa
     1081 tggttgttgt tcatagatat tttccctcct gttttttggt caaccgaaag atcagttttt
     1141 agaacttcct cggctatttt gcggggcggc gcgcgagcag gaatcccgcc acgaccaaga
     1201 cgagcgccag gccaatccga atttcccact ccaaagtctt ggaagcctcg ccgagagtga
     1261 cctcgacgaa gtcgtcttcc aggatcatct ttcccgccac ctcgcccaaa atcgccgcgc
     1321 cgatgtacgt gggccagcga aagcggctca tgagaaccgc gatcaatgca gcccctgcga
     1381 cgacgatggg gatgctgagc ccgaggccga agagcacgag gaaaaagctt cccccggagg
     1441 ctccggcaat cgccagtatg ttatccgtgg acatgacgag gtccgcggca ataatgatgc
     1501 ggatcgcctg gggtaaggtc gccccttgcc gcatcgcggg cgtgctccga cgatcctgac
     1561 ggagaagctt acaggcaatc cagacgagga ccagaccgcc cgcgagctgc acgtaaggaa
     1621 aaaacaagaa tcgcgccgcc gcgaaagtga gcgcgacccg agcaatgacg gctcccaagg
     1681 cgccgagaca aactccgatc cgctgctggc gcgcggggag cgcgcgcacc gccatgccaa
     1741 tgacgactgc gttatccccc gcgagcgtca gatccaggac aacgatcttg agccaactca
     1801 ggatgaagtg ccaatccaat cccggcaagc ctctcggctg gttcgccagc cgctcgaaaa
     1861 ctggagatat cgcggctgca tcaaccagcg gcccctttgc tcatgcggtc gtcgctcacc
     1921 gaaacggaat cgcgccgcat cagactattt ccagccgagc tcgccgaggg cctgttccag
     1981 gtaactggaa tcgacgtatt ttgccggatc gggcagcggc cctttcaggt cggcctgctc
     2041 ggcgtaaacc gcaatgaccg tcttcagccc gtccagatcg atgcgcagca gcggatccca
     2101 ggcgcgctga tcgacgtagt agtcgagtcc ccggcgggcg agcgcaggtt tcatgtcgag
     2161 ttcctcggct aaaaattccg ccgcctcctg cctgtgttcg tcgatccacc tgcgcgcttt
     2221 gagcagcgcg cgcaggaatc gtatcacctc ctctcgatgg g
//
LOCUS       DASSRL010000108         6497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_650462, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000108 DASSRL010000000
VERSION     DASSRL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650462"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="fumC"
                     /locus_tag="VFL49_02285"
     CDS             complement(<1..1354)
                     /gene="fumC"
                     /locus_tag="VFL49_02285"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET7910834.1"
                     /translation="MGKTRDRSSKTRIESDAFGPLKIPANKLWGAQTQRSLHNFRIGA
                     ERMPIEIVHALGLIKRAAAEVNRDLGSLDRKRASAIAVVAQEIADGKLDTHFPLLVWQ
                     TGSGTQTNMNVNEVIANAANLNLGGKLGAKAPVHPNDHVNMSQSSNDSIPTAMHIAAA
                     VEIERHLKPALTHLFRALDKKAKEFSGIIKIGRTHTMDATPITLGQEFSGYAGQVKSS
                     LARLTMAQRELYPLAQGGTAVGTGLNSKPRFAKAIAKRIARLTKLPFTSAPNKFEHMA
                     GHDAYVFVHGALNSAATALFKIANDIRLLGSGPRSGLGELILPSNELGSSIMPGKTNP
                     TQSEALTMVCCQVFGNQTAITVAGSQGHFELNVYKPVLAFCMMQSIRLLSDAVLSFTD
                     NCIIGIRANEARIRELMQRSLMLVTALSPKIGYDKAAQVAKTAHANGTTLREEAIRLG
                     F"
     gene            complement(1443..1997)
                     /locus_tag="VFL49_02290"
     CDS             complement(1443..1997)
                     /locus_tag="VFL49_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HET7910835.1"
                     /translation="MVDQIRYDLLTQQALRGVVRSVLTDTAKKGLPGDHHFYISFDTK
                     TDGVHMSDRLRTQYPDEMTIILQHQFWDLKVSEHEFEVGLSFGGIPERLTVPFDSING
                     FFDPSVQFGLQFEEVAEGEERSPPANVQEPVSKRKRSPTSPPPAATTDTPARATAPSS
                     APKREKPEKPAGGEVVRLDRFRKK"
     gene            complement(2118..2477)
                     /gene="ssrA"
                     /locus_tag="VFL49_02295"
     tmRNA           complement(2118..2477)
                     /gene="ssrA"
                     /locus_tag="VFL49_02295"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(2502..2894)
                     /locus_tag="VFL49_02300"
     CDS             complement(2502..2894)
                     /locus_tag="VFL49_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HET7910836.1"
                     /translation="MGPGSLLLNVLWILFGGFYMALGWLIAAVIMVLTIIGIPWARAA
                     FTIAAYTLLPFGHTVVSRPTGTGSGFLGFVGNLIWLLLAGWWLALGHLLTALLLAITL
                     IGIPFAWAHLKLAGLALWPIGKDIVSTN"
     gene            3028..3597
                     /locus_tag="VFL49_02305"
     CDS             3028..3597
                     /locus_tag="VFL49_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET7910837.1"
                     /translation="MSDSVAPSRSIAARPTLPELFRAFVVVSISGFGGALPWARRMIV
                     DQRGWMTAEEFNEAFALSQFLPGPNVVNFSVVFGARFGGAAGAAVALAGLMGPPLVIV
                     TVLAFLYARFGDLEMLSRVLAGITAAAVVLLIAVVTKMAAPLFRKRWDWAPLIAIAAF
                     IGVAIMQWPLPLVFAILAPVSIALAWVRR"
     gene            3594..4121
                     /locus_tag="VFL49_02310"
     CDS             3594..4121
                     /locus_tag="VFL49_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET7910838.1"
                     /translation="MNENPIIPLFGYFALLSLFAVGGANAAIPEMHRIAVDVMHWMSD
                     RQFADSFAIAQISPGPNVIIVTLIGYHVAGLAGAAVATVAMCGPTCVFAFLIARTWDH
                     FKEARWRIAIQAGLVPVSLGLIGASAFVLARSADHNLYAALITAVTAIVAFATRINPL
                     WLFAVGGLLGLSGWL"
     gene            4246..4767
                     /locus_tag="VFL49_02315"
     CDS             4246..4767
                     /locus_tag="VFL49_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET7910839.1"
                     /translation="MAEGVRPSPNGSGLRFKVRGPREFYGGLALIALAIVAIWASGDL
                     PGMHGFAFGPGTAPRLFASLLAICGAMIALSGLLFDGPPLDSYAIRGPAWVILAILAF
                     AGMIRGVHFSFATIPPLGLIPSTFAAFMISIFGSSEMRWIESLMAATAMTAFCVALFV
                     YLLQLPFQLWPWY"
     gene            4783..6288
                     /locus_tag="VFL49_02320"
     CDS             4783..6288
                     /locus_tag="VFL49_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET7910840.1"
                     /translation="MSELFSNLALGFSVAAHPYNIGFCLLGALVGTLVGVLPGIGTVA
                     TVAMLLPITFGLPPVGALIMLAGIYYGAQYGGSTTSVLVNIPGEAGSVVTCLDGHQMA
                     KQGRAGAALAIAAIASFFAGCVATVLVAALGTPLTSIALLFGPAEYFSLMVLGLIFAV
                     VLAKGSVLNAIAMILTGLLLSMMGSDLETGAGRMTFDIAELSDGIGFSNVAMGVFGFA
                     EIIRNLEMSQESRDIVKGKIKGLMPTRKDLVDASGAIARGTILGSLLGILPGGGAVVS
                     SFAAYTFEKRISKNPERFGRGAIEGVAAPEAANNAAAQTSFIPLLTLGIPPNAVMALM
                     VGAMTIHGIVPGPQVMTKQPELFWGMVASMWLGNLMLIIINLPLVGVWVSLLRVPYRL
                     LYPSIIVFCCIGIYSINNSPTDVVISAIFGLVGYWLTKHDFEPAPLVLAFVLGPLMEE
                     NLRRAMLIARGDATVFITRPISGVLIALAVGLLVIAALPMIRRRRDEVFVE"
     gene            complement(6315..>6497)
                     /locus_tag="VFL49_02325"
     CDS             complement(6315..>6497)
                     /locus_tag="VFL49_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910841.1"
                     /translation="AALITMPIYGGGAYDENGYQPFKAPVPIFAKKWDANITAFSIQL
                     VILAAGILTVSGAVAG"
BASE COUNT         1187 a         1839 c         1992 g         1479 t
ORIGIN      
        1 caaagccaag gcgaatcgcc tcctcgcgca gcgtcgttcc attggcatgt gccgtcttcg
       61 caacctgagc ggctttgtca tatccgatct tcggagagag ggcggtgacc agcatgaggg
      121 atcgctgcat cagttcgcga atgcgcgctt cgttggcgcg aattccaatg atgcaattgt
      181 cggtgaaaga gagcactgcg tcgctcagca accggatcga ttgcatcatg caaaaagcga
      241 gtaccggctt gtaaacgttg agttcaaaat gcccctggct gccggcgacc gtaatggccg
      301 tttgatttcc gaatacctgg caacagacca tcgttagggc ttcgctttgc gtcggatttg
      361 tcttgccggg catgatcgaa gatccgagct cgttgcttgg aaggatgagt tcgccgaggc
      421 ccgagcgcgg gccggagccc agcaggcgaa tatcgttcgc gatcttgaac aatgccgtgg
      481 cggcagagtt gagggcgcca tgtacgaaca cataggcatc atgaccggcc atgtgctcga
      541 acttgttagg cgcgctcgtg aacggcaact tcgtcagtcg agcgatccgt ttcgcaatgg
      601 cctttgcaaa tcgtggcttt gaattcaagc cggtgcccac agcggtgccg ccttgcgcga
      661 gcggataaag ttcgcgctgc gccatcgtca ggcgggcaag agatgatttc acctgcccgg
      721 catagccgga gaattcctgt ccgagggtaa ttggcgtcgc atccatcgta tgcgtgcggc
      781 cgatcttgat gatcccggaa aattccttcg cctttttgtc gagcgcccga aacaggtgcg
      841 tcaacgccgg cttcaggtga cgttcgattt cgacagccgc tgcaatatgc atagcggtcg
      901 gtatggagtc gttcgacgac tgactcatgt tcacgtgatc gtttggatga accggcgcct
      961 ttgcaccgag cttgccgccc agattcagat tcgccgcatt ggcaatcact tcgttgacat
     1021 tcatgttggt ttgagtgccc gacccggtct gccacaccag cagcggaaag tgcgtgtcga
     1081 gcttgccgtc agcgatctcc tgggccacga cggcaatggc tgatgcgcgt ttccggtcga
     1141 gcgagccgag atcgcgattg acctcggccg ctgcccgctt gatcaggcca agcgcgtgca
     1201 cgatttcgat tggcatccgt tcggcgccga tacggaaatt gtgcagcgag cgctgcgtct
     1261 gggctcccca cagcttgttc gccgggattt tcagcggccc gaacgcgtca ctttcaatgc
     1321 gtgtctttga cgaccgatcg cgagtcttgc ccatatgatt tggtccgttg tacgggactg
     1381 cccaacgaac ctatgccgaa tagcggcggg cacaagtacg ctcgtaaacc gagacgcgca
     1441 gactattttt ttcggaatcg gtcgagcctg acgacttcgc cacctgccgg cttttcgggt
     1501 ttttcgcgtt ttggtgcgga tgagggggcg gtcgcccgcg ccggcgtatc ggtcgtggcg
     1561 gcgggtggcg gactggtcgg cgatcgtttt ctcttgctta cgggttcctg cacattggca
     1621 ggtggcgacc gttcctcgcc ttcggcaact tcctcaaatt gcagaccgaa ctgcaccgaa
     1681 gggtcaaaga agccattgat agaatcgaat ggcaccgtga gacgctccgg gattccaccg
     1741 aaagaaagcc ccacctcgaa ctcgtgctcg ctgaccttca gatcccagaa ttgatgctgg
     1801 aggatgatgg tcatctcgtc cgggtattgc gtgcgcagcc gatcggacat gtgcacgccg
     1861 tcggtctttg tgtcgaatga gatataaaaa tgatggtcgc cagggaggcc tttcttggcc
     1921 gtatcggtca gtacgctgcg gaccacgccg cgcagggcct gctgggtcaa aaggtcatag
     1981 cgaatttggt cgaccatgcg aatgagtgtc gtttatggat tcgcacaatg ctagcgcggg
     2041 caatgcgctc cgcgctatcc ccgtggggcc cgtgcaaagc cgcattgtca tagatggata
     2101 tcgcgcggcc aacgagaagt ggaggcttct gttgccaggt gcctccggac cccgcctgac
     2161 ggagctaacc cgtcagggct ttagatcggt ctttcaaacc gcttacgcgg cctgagcaac
     2221 cggagcatag ttgtcgttgg caactatgaa tatggcccga tatcggcggt accatgccga
     2281 gcgaaagcac gccctttacg ccctcgtcga tcctatttcg cccccgccgc tgccgccccc
     2341 cggttccggg cggcactggt ggaggcgccg ggtactgccc ccgggtccga tgggtttatt
     2401 acgacggcca tttatcgcca tagccgggtt tccccggcag ctgaaatata ggaggcaaag
     2461 gttaccgaag gaagagcggc cggtggccga taattcgggg gttaattcgt gctcacgatg
     2521 tctttgccga tcggccaaag cgcaagaccg gcaagcttga gatgcgccca tgcaaaagga
     2581 atcccgataa gggtgatcgc gagcagcaag gccgtaagca aatgaccaag ggcgagccac
     2641 cacccggcca gcagcaacca gatcaaattg ccgacgaagc caaggaatcc gctgccggtg
     2701 ccggtcggcc gtgacaccac ggtgtgaccg aagggaagga gtgtataggc cgcgatagta
     2761 aaagccgcac gcgcccaggg aatgccgatg atggtgagaa ccataattac ggctgcgata
     2821 agccagccca atgccatgta gaagccaccg aacaggatcc acagaacgtt caggagcaaa
     2881 ctacccggtc ccatagctag ccactcacaa tcgaccgaaa gaccgagctt gcccgatttt
     2941 gcgcgcgacc gcgagccgaa ggttaggcgc ggcggctatg cacaatccat ctagtcgtta
     3001 cccgtggcgc gggataatgc tgcgatcatg tccgattccg ttgcgccatc ccgatcgata
     3061 gccgcacgac ccacgctacc tgaactgttc cgcgcctttg tcgtcgtctc catctcgggt
     3121 ttcggcgggg cgttaccatg ggcgcggcgt atgatcgtag accaacgcgg gtggatgacg
     3181 gcggaagagt tcaacgaagc gttcgcgctg tctcagttcc ttccaggccc caatgtcgtg
     3241 aacttctcgg tcgtgttcgg cgcgcgcttt ggaggagcgg ccggagcagc ggtggcgctt
     3301 gccggactta tgggcccccc gttggtcatt gtgacggtgc tcgcttttct gtatgcgcgg
     3361 tttggcgatc tcgaaatgct cagtcgtgtg ctggcgggca ttactgccgc cgcggtcgta
     3421 ctgctgatcg cggtcgtgac caaaatggcg gcgccattgt tccgcaaacg ttgggactgg
     3481 gcacctctca tcgcgattgc cgcgttcatt ggcgttgcaa tcatgcaatg gccgctgccg
     3541 ttagtatttg cgatacttgc ccccgtcagc atcgcgctcg cgtgggtgag acgatgaacg
     3601 aaaatccaat catcccgttg ttcggctatt tcgcgctgct gtcgttgttc gcggtcggtg
     3661 gcgctaatgc ggcaattccg gaaatgcatc gcatcgccgt tgatgtgatg cattggatga
     3721 gcgaccggca gttcgctgac agtttcgcga ttgcgcagat ctcgccgggg ccaaacgtga
     3781 tcatcgtcac gctgatcggc taccacgtcg cgggtctggc gggtgcagcc gtggcgacgg
     3841 tagcaatgtg cggcccgact tgtgtgttcg cctttctgat tgcacgcacc tgggaccatt
     3901 tcaaagaggc acgctggcgt atcgccatac aggccggcct ggtgccggtt tcgctcggat
     3961 tgattggcgc gagcgccttt gtgcttgccc gaagcgcaga ccacaatctt tatgcggcct
     4021 taattacggc ggttacggca atagttgcat ttgccacacg aattaatccg ctttggctgt
     4081 tcgcggtcgg gggtcttttg ggcctctcgg gatggctgtg atggacgatt gcaacatccg
     4141 tgcttccggc acggccttgt cgtatgctag ggatcagacg tggggtgcag gcagcggcgt
     4201 aagtacaaaa ccaagaagta agaacaacca ggcgggagga caaagatggc cgagggggtc
     4261 agaccctccc cgaacgggag cgggttgcgt ttcaaggtcc ggggcccgcg tgaattctac
     4321 ggcgggttgg cgctaattgc tcttgccatt gtggcgatct gggcttcggg cgaccttccg
     4381 gggatgcatg gatttgcgtt cggtcctggc actgcgccac gcttgtttgc cagtctcctt
     4441 gcgatttgcg gcgcgatgat agctttgagc ggattgctct ttgatggtcc gccccttgac
     4501 agctatgcga tccgcggtcc ggcctgggtc atactcgcga tccttgcttt tgccggcatg
     4561 atccgcggcg tgcatttcag ctttgcgacg atcccgccgc tgggactgat cccttcgact
     4621 tttgccgcgt tcatgatctc gattttcggc tcgagcgaaa tgcggtggat cgagagtctt
     4681 atggccgcaa cagcgatgac cgcgttttgc gtggcgctat tcgtatacct gttgcagctg
     4741 ccgtttcagc tttggccctg gtactgagaa aaaagtgcag ccatgagtga acttttctcc
     4801 aatctcgcgc tcgggttctc agtcgcggcg catccctaca acatcggttt ctgtctcctt
     4861 ggtgcgttgg tgggcacatt ggtcggtgtg ctcccgggaa tcggcacggt ggccaccgtt
     4921 gccatgcttc tgccgattac gtttggattg ccgccggtgg gtgcgctgat catgctcgcc
     4981 gggatctatt acggtgcaca gtacggtggc tcgaccacat ctgtccttgt gaatattcca
     5041 ggggaggcag ggtcggtcgt tacgtgtctc gatggccacc agatggcaaa gcagggccgg
     5101 gccggcgctg cgcttgcaat cgcggcaatt gcttcgttct tcgccggttg tgtcgcgaca
     5161 gtgctggtcg ccgccctcgg cacgccactg acgtcgatag cgttgttgtt cggacccgcg
     5221 gaatattttt cgctgatggt gctgggcctg atttttgcgg tcgtgctcgc aaaaggctct
     5281 gtcctgaacg cgatcgccat gatcctgacc ggcttgttgc tctcgatgat gggatcggat
     5341 ctcgagacgg gcgctggccg catgaccttc gacatcgccg agttgtcgga cggcatcgga
     5401 ttcagcaacg tggcgatggg cgttttcgga tttgccgaga tcatccgcaa tcttgaaatg
     5461 tcccaggaaa gccgcgacat cgtgaaaggg aaaatcaaag gtctgatgcc gacccgcaag
     5521 gatctggtcg acgcttcagg cgcgattgcc cgcggcacca ttctcggttc gttgcttgga
     5581 attctgcccg gtggcggtgc agtcgtatca tcgttcgccg cctatacatt cgagaagcgc
     5641 atctcgaaaa acccggaacg tttcggacgc ggcgccattg agggcgtggc cgcgcctgaa
     5701 gcagccaaca atgccgccgc gcagacctcg ttcattccgt tgttgacatt gggcattcca
     5761 ccgaacgccg tcatggcctt gatggttggc gcaatgacca tccacggcat cgtgccgggg
     5821 ccgcaggtca tgacgaaaca gccggaattg ttctggggca tggttgcctc gatgtggctc
     5881 ggcaacctca tgctgatcat tattaatctg ccgttggtgg gcgtgtgggt cagcctgctg
     5941 cgcgtgcctt atcggctact gtatccctcg atcatcgtgt tctgttgcat cggcatttat
     6001 tcgatcaaca actctccgac cgacgtggtg atctcggcga tcttcggcct tgtgggatat
     6061 tggctcacca aacacgactt cgagccggcg ccgctggtgc tggcattcgt gctcggcccc
     6121 ctgatggaag aaaacctgcg gcgggcgatg ctgatcgcac gcggcgacgc gaccgtattc
     6181 atcacgcgcc caatttcggg tgtgctgatt gctctcgcgg tgggcctgtt ggtgatcgcc
     6241 gctttgccga tgatccgcag acggcgcgac gaggtcttcg tcgaatagtt ggagtggcgc
     6301 ggatcgcgtg ccacttaacc ggcgacggcg ccggacaccg tcaatattcc cgctgccagg
     6361 atcacaagct gaattgagaa ggccgttatg ttggcgtccc acttttttgc gaagatcggc
     6421 acgggtgcct tgaacggctg atagccgttt tcgtcgtacg cgccgccccc gtaaatcggc
     6481 attgtgatca gcgccgc
//
LOCUS       DASSRL010000109         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1192757, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000109 DASSRL010000000
VERSION     DASSRL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192757"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFL49_02330"
     CDS             complement(<1..444)
                     /locus_tag="VFL49_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7910842.1"
                     /translation="MPYATTPDNVKLYYEEVGQGAPILFVHEFAGDHRSWERQLREFG
                     KHYRCIAYCARGYTPSDIPSDAKAYSYMHVMRDAVGMLDHLKIDKAHLIGLSMGAYTA
                     LQVALNHPRRVRSLVLAGAGSGSERWYTADFHKRSRDLATQFEREG"
     gene            complement(444..1925)
                     /locus_tag="VFL49_02335"
     CDS             complement(444..1925)
                     /locus_tag="VFL49_02335"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET7910843.1"
                     /translation="MTMPPRSRKSATFVERHGLWSEAQAKAAAQVERIIKKEKLEVVR
                     FSFADQHGVLRGKTLLASEVASAMRTGVTMTTTLLAKDTAHRTVYPVFTPGGGFAMEE
                     MQGGGDFVMIADPATFRILPWANKTGWLLCDIHFTNGKPVPFSARARCRDALNTLANA
                     GFDFLAGLEVEFHLFKIENPRLAPADATWPPEAPDVSLLTQGYQYLTESRYDMLDPAL
                     EILRHGIEQLGLPLRSVESELGPSQCEFTFRPQAGLASADTMVLFRAAAKQIARRHGL
                     LATFMCRPALPNVMSSGWHLHQSLLEAKSRRNAFVFDHPDVLSTVGRYYLGGLLAHAR
                     AAAAFTTPTINGYKRYRAYSLAPDRAIWGRDNRGVMIRVLGQPGDPATRLENRVGEPA
                     ANPYLYVAAQIYSGLDGIAHRLDPGPSADTPYEAAAVLLPKNLSEALTALRADETFRA
                     GFGGGFIDYFTHIKAAEIERFQKEAADQTDVTPWEQKEYLDLF"
BASE COUNT          388 a          597 c          644 g          377 t
ORIGIN      
        1 gccttcacgc tcgaactgcg tcgcaagatc gcgcgagcgt ttgtggaaat ctgcggtgta
       61 ccagcgctcg ctgcctgatc ccgcgcctgc gagcaccagc gagcgcacgc gccgcggatg
      121 atttagtgcg acctgcagcg ccgtgtaggc gcccatcgac agcccgataa gatgcgcctt
      181 gtcgatcttc agatgatcga gcatccccac ggcatcgcgc atcacgtgca tgtagctata
      241 cgcctttgca tcggacggaa tatccgacgg cgtgtatccc cgcgcgcaat aagcgatgca
      301 gcggtagtgc ttgccgaatt cgcgcagctg gcgttcccag ctgcgatggt cgccggcaaa
      361 ctcgtgtacg aagaggatcg gcgcaccctg ccctacttcc tcgtaataca gcttcacgtt
      421 gtcgggcgtc gttgcatacg gcatcagaac aagtccagat attccttctg ctcccagggc
      481 gtgacgtccg tctgatcggc cgcttccttt tgaaagcgct cgatctcggc cgctttgatg
      541 tgcgtgaaat agtcgatgaa cccgccgccg aagccggcgc ggaaggtttc gtcggcgcgc
      601 agcgctgtca aagcctcgct gagatttttc ggaagcaaca cggcggccgc ctcataaggt
      661 gtgtcggccg acggtcccgg atcgagcctg tgcgcaatgc catcgagccc ggaatagatc
      721 tgcgcagcga catacagata cggattcgct gcaggctcgc cgacgcgatt ctcaagacga
      781 gtggcaggat cgccgggctg gcccagcaca cggatcatga cgccgcgatt gtcgcgtccc
      841 cagatggcgc ggtcaggcgc gagcgaatag gcgcggtagc gcttgtagcc attgatcgtc
      901 ggcgtcgtaa aagctgccgc cgcgcgcgca tgcgccagga gaccgccaag ataatagcgg
      961 ccgacagtcg agaggacgtc agggtgatcg aagacaaagg cgttccgccg cgattttgct
     1021 tcgagcaagg attgatgcag atgccagccg ctcgacatga cgttcggcag cgccggacgg
     1081 cacatgaagg tcgcaagaag gccgtgccgg cgcgcgatct gttttgcagc ggcacgaaag
     1141 agcaccatgg tgtcggcgct ggccagaccg gcttgcgggc gaaaggtgaa ttcgcactgg
     1201 ctcggcccga gctcagattc gaccgaacgc agcggcaggc cgagttgctc gatgccgtgg
     1261 cggagaattt ccagcgcggg atcgagcatg tcgtagcgcg actcggtgag atactgataa
     1321 ccctgcgtga gcaggctcac atccggggct tccggcggcc aggtcgcgtc ggccggcgca
     1381 agacgcgggt tctcgatctt gaacaggtgg aattcgacct cgagcccggc gaggaaatcg
     1441 aaaccggcat tggccagcgt gttgagcgcg tcgcggcagc gggcgcgcgc ggagaacggt
     1501 accggcttgc cgttggtaaa gtggatgtcg cacaagagcc agccggtctt gttcgcccag
     1561 ggcaaaatgc ggaatgtcgc ggggtcggca atcatcacga agtctccgcc gccctgcatt
     1621 tcctccatcg cgaagccgcc gcccggcgtg aacaccggat agaccgtacg gtgcgcggta
     1681 tccttggcaa gcagcgtggt agtcatggtg acgccggtgc gcatcgcgct cgcaacttcg
     1741 gacgcaagca gcgtcttgcc ccgcaatact ccgtgctggt cggcgaatga aaagcgcacg
     1801 acctcgagct tctctttctt gatgatgcgc tcgacctgcg cggcggcttt tgcctgcgct
     1861 tcgctccaga gaccgtggcg ctcgacgaag gtggccgatt tacgcgagcg cgggggcatt
     1921 gtcattggcg aatagtaaat ggcgaatagg gaatggaaaa aagcatagta gtcttttcgc
     1981 gttcgctact cgctattcac tattcg
//
LOCUS       DASSRL010000110         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1542649, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000110 DASSRL010000000
VERSION     DASSRL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542649"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..663)
                     /gene="maiA"
                     /locus_tag="VFL49_02340"
     CDS             complement(13..663)
                     /gene="maiA"
                     /locus_tag="VFL49_02340"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HET7910844.1"
                     /translation="MKLFSFWRSLATYRVRIALNLKGLSAEVVEVNLIKGHQREEAFR
                     KVNPMMAIPALVDGTGPALYESLAIIEYLDETHPNPPLLPKDPRGRGRVRGLSQIVAC
                     DSHPLIVPRVREYLAHEFKVDEAGVTKWAQHWHRAALGALEEHLAGEKETGMYCHGEQ
                     ITVADICVASQVAGANFFKVDLAPYPTVSRIAATCAKNEAFARAHPLKQPGAPATV"
     gene            complement(679..1566)
                     /locus_tag="VFL49_02345"
     CDS             complement(679..1566)
                     /locus_tag="VFL49_02345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7910845.1"
                     /translation="MRIGVAGLGVMGAAIAARLMEVGHEVTVWNRTAEKTKPLADTGA
                     KVASTPAQLASAVEAAITMLTDAAAIDAVYNGSNGLLAGDVKGKLLIEMSTVPPKVPL
                     ALVPKVRAKGAAFVECPVGGSTAPARQGKLLGLMGAEPADAERAKPLLNQLCRRLEHC
                     GPVGTGASMKLAINLPLMVAWQAYGEAFAIARAAGWEPKRLLDLFVDTNGANPALKNR
                     AEMIVTMFEGRDPGPTTFSIANGVKDLRTMVETGEANGADMRVTKATLTGFEDATKNG
                     FGGGDGSRMSVYWATRNKA"
     gene            1716..>2462
                     /locus_tag="VFL49_02350"
     CDS             1716..>2462
                     /locus_tag="VFL49_02350"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7910846.1"
                     /translation="MAARRRKGTVGVIGLGIMGGAFAKNLVKAGWHVVGYDTNAARRR
                     EAKRAGVDIAQNAVELAAAAPTILTSLPKAEALADTARKIAAAKLRHKLFVEMSTFKI
                     SDKEKAATVLRKAGHQMIDCPVSGTGAQARNRDLVFYASGDSKLIAKIRPLLSAFGRG
                     VFNVGPFGNGSRMKYVANLLVAINNVASAEAMVLGMKAGLDPRMIVDLITAGAGNSRV
                     FELRAPMMAKGRYDDVTMKISVWDKDMQVIG"
BASE COUNT          477 a          780 c          739 g          466 t
ORIGIN      
        1 gatcatgcgc ccctaaaccg ttgccggcgc accgggctgt ttgagcggat gtgcgcgcgc
       61 aaacgcctcg ttcttcgcgc aggtggcggc gatacgggac acggtagggt acggcgccaa
      121 atcgaccttg aagaaatttg ctcccgcgac ctggcttgcg acacagatgt cggcaactgt
      181 gatctgctcg ccgtgacaat acataccggt ttccttttca ccggcaagat gctcctccag
      241 cgcgcccagt gccgcgcggt gccagtgctg cgcccatttc gtaacgccag cttcatcgac
      301 cttgaactcg tgcgcaagat actcgcgcac gcgcggaaca atcagcggat gagaatcgca
      361 ggcaacaatt tgcgagagcc cgcggactcg cccgcgcccg cgtgggtcct tcggcaacaa
      421 cggcggattg gggtgcgtct cgtcgagata ctcgatgatc gccagcgact cgtagagcgc
      481 gggcccggtg ccgtcgacta aggccggaat cgccatcatc ggattgactt tgcggaatgc
      541 ttcctcgcgc tgatggcctt tgatcaggtt gacttcgact acctcggccg aaagtccctt
      601 gaggttcagc gcaatgcgca cgcgataggt cgcaagcgag cgccagaatg aaaagagttt
      661 cacggagtcc tcctatcctc aggctttgtt gcgcgtggcc caatacacag acatgcgcga
      721 gccgtcgccg ccgccaaagc cattctttgt cgcatcctca aacccggtga gcgtcgcctt
      781 tgtcacgcgc atgtcggcgc cgttcgcttc gccggtttcc accatcgtgc gcagatcctt
      841 tacgccattg gcgatgctga acgtggtcgg ccccggatcg cggccctcga acatcgtcac
      901 gatcatctcg gcacgattct tcagggcagg atttgccccg ttggtgtcga cgaacagatc
      961 gagcagccgc ttcggctccc agccggcggc gcgagcaatg gcgaaggctt cgccataggc
     1021 ctgccaggcc accatcagcg gcaggttgat tgcaagcttc atcgacgcac cggtgccgac
     1081 cggcccgcaa tgctcaagcc gccggcagag ctgattgagc agcggcttgg cgcgctcggc
     1141 atccgcaggc tcggcaccca taagcccgag caattttccc tgacgcgccg gcgcggtcga
     1201 accgccgacc ggacattcga cgaacgccgc gcccttcgcg cgcactttag gcaccagggc
     1261 cagtggcacc ttcggcggca cggtactcat ttcgataagc aatttgcctt ttacatcgcc
     1321 tgcgagcaga ccgttcgatc cgttgtaaac ggcgtcgatc gccgcggcat cagtcagcat
     1381 cgtgattgca gcctcgacag cgcttgcaag ttgcgccggc gtcgacgcaa ccttggcgcc
     1441 ggtatccgcc aacggtttgg ttttctccgc cgtgcgattc cataccgtca cctcgtggcc
     1501 gacttccatg aggcgcgctg caatcgccgc ccccatgact ccaagacctg caacaccgat
     1561 ccgcatgcgt cctcctgttc ttttgcgggg acgataggcg atcaagcacg tgcttgaaag
     1621 cgggcatgct gcgacccgcg cgactcgaca gccctatacc caacgcataa gagagaatgg
     1681 caaacggatg caatttggca tcggggagac ggtgaatggc ggcaagacgg cgcaagggca
     1741 cggtcggcgt gatcggcctc ggcatcatgg gcggtgcctt cgcgaagaac ctggtcaagg
     1801 ctggctggca tgttgtcggt tacgacacca atgcggcgcg ccgtcgtgaa gcaaaacgag
     1861 ccggggtcga tattgcgcaa aacgcggtcg agctcgcggc cgctgctccg acaatcctga
     1921 cgagccttcc caaggcggaa gctcttgccg atacggcgcg caagatcgct gctgcgaagc
     1981 tcaggcacaa gttgttcgtt gaaatgagca ccttcaaaat ttcagataag gaaaaggccg
     2041 ccaccgtgct gcgcaaggcg gggcatcaaa tgatcgactg cccggtcagc ggcaccggcg
     2101 cgcaggcgag aaaccgcgat ctcgtgttct atgccagcgg tgactccaag ctcatcgcga
     2161 aaatccggcc gctgctttca gcgttcgggc gcggggtgtt caatgttggg ccgttcggca
     2221 acggcagccg gatgaaatac gtcgccaacc ttctggtcgc catcaacaat gtcgcctcgg
     2281 ccgaagccat ggttctcggc atgaaagccg gcctcgatcc ccgcatgatc gtcgatctga
     2341 tcaccgctgg cgccggcaat tcgcgtgtct tcgagttgcg cgcgccgatg atggcgaaag
     2401 gccgctacga cgacgtgacg atgaagatct ccgtctggga caaggacatg caggtgatcg
     2461 gc
//
LOCUS       DASSRL010000111         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1437704, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000111 DASSRL010000000
VERSION     DASSRL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437704"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..575)
                     /locus_tag="VFL49_02355"
     CDS             complement(12..575)
                     /locus_tag="VFL49_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7910847.1"
                     /translation="MAGKTIHAAGGIVVRNGARPLIAIVQRAKDELWVLPRGKLKRDE
                     SPLAGARREVVEETGHRVRVREFVGAITYRARGRPKVVQFWRMQAEVNPSRDVMEDIM
                     AVEWLPLSAAVRKLSYPLERLFLSSVGRHILRSRRKTRSPGRKSKSRRRASKGHPHRG
                     ASRARKSARGGRPHSTILQRVLGHLPS"
     gene            complement(685..1668)
                     /locus_tag="VFL49_02360"
     CDS             complement(685..1668)
                     /locus_tag="VFL49_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910848.1"
                     /translation="MTGHDRRKFLQFTAGMLATPMLSGPASAQTTWPTARSIRAIVPF
                     SAGSTIDILGRIVLDPLSKQLGQTIVVENRGGAGGSLGSAAVAKADPDGYTILINAAA
                     HSGAPAAYPNLPYDAANDFSAVASFGNVPNVLLVAPSKGIRTVQELVAKAKAGSLSFA
                     SAGVGSATHWAAERFNVSAGLKVTHVPFRGGPEALTEVMTGRVDYIFIGISSGLPFIQ
                     NKQLVPLAVSSAKRSPTLPDVPTTLEAGYKDSDFNYWNGMLVPAKTPRPIVDRLYTET
                     TKVLTLPDVQQKLKIQGVEPAPLTPAEMDAMIRREIALNLKIAKEAGLKFN"
     gene            complement(1701..1922)
                     /locus_tag="VFL49_02365"
     CDS             complement(1701..1922)
                     /locus_tag="VFL49_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910849.1"
                     /translation="MLKSDTGLVIALAAVLAVASPPALAKGQKSSTPKHTVKGEHIKE
                     GTITVRKKSKSKPAISEIKVTKPVDKSSP"
     gene            2064..2459
                     /locus_tag="VFL49_02370"
     CDS             2064..2459
                     /locus_tag="VFL49_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7910850.1"
                     /translation="MADFKWDHVHLRTPDPEATAQWFERMFGAEVIRSTQQGKPRVDI
                     RLGGAMIFTAQVNSGDGVNPAPVTPYQGLDHFGLAVSGIDAIAADLRKKGVEFTKEPH
                     SPRPGIKVCFLRGPQGISIELLDRDPKYS"
     gene            2553..2942
                     /locus_tag="VFL49_02375"
     CDS             2553..2942
                     /locus_tag="VFL49_02375"
                     /inference="COORDINATES: protein motif:HMM:NF017837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF930 domain-containing protein"
                     /protein_id="HET7910851.1"
                     /translation="MRAFGLAVVAATISAATATAADGRFERTLKMLAPEERLEQLCDY
                     TAMMRIRNEQKQFRPDRAVANAMTEPKATGNTLEVTGGAFRSRMKWYALSYRCTATPD
                     RMSVVAFHFTIGAEIPEAKWSSYGLWD"
     gene            complement(2951..3544)
                     /locus_tag="VFL49_02380"
     CDS             complement(2951..3544)
                     /locus_tag="VFL49_02380"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7910852.1"
                     /translation="MLRLRVFALAILLVPAAPAMSQTLLPSSTPTMQDVDPGVDHTAK
                     DVDVSPEFRRKSVFYRTEQPVGTIIVNTADRYLYLIMGNGVALRYGIGVGRDGFQWGG
                     IQKITLKREWPDWVPPPEMIQRQPYLPRWMAGGPGNPLGARAMNLGATVYRIHGTNAP
                     ETIGQAVSSGCFRLVNEDVMDLYDRVPIGTKVVVQHQ"
     gene            complement(3591..3836)
                     /locus_tag="VFL49_02385"
     CDS             complement(3591..3836)
                     /locus_tag="VFL49_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HET7910853.1"
                     /translation="MVEVTYEIVEHDGGWAYKVDGVFSEPYPSHAAALAAAQRAAAEQ
                     TVPGHTETIEYEDEKGRWHTETARGSDRPLTQIKDKD"
     gene            complement(3966..4499)
                     /locus_tag="VFL49_02390"
     CDS             complement(3966..4499)
                     /locus_tag="VFL49_02390"
                     /inference="COORDINATES: protein motif:HMM:NF021584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HET7910854.1"
                     /translation="MASKNAAKKTATGFAQILNAARAAGLTASPPEPEPAKAGESALS
                     FLALNDRAMSAAVQNAREALSGFLALARHPGPAMEHFAVKIALVGDGGPELFWIYPFA
                     HVGERFIGQVNSSPLTLAGPKKGDTIAFSRRDIVDWAYTEAGAIKGNFSARAILKAAL
                     PHDRTAFRRRFGLEFDF"
     gene            complement(4608..5144)
                     /locus_tag="VFL49_02395"
     CDS             complement(4608..5144)
                     /locus_tag="VFL49_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7910855.1"
                     /translation="MTSFFSTNWFDLAIYVCLFAAVVMGFMTGLLRSLATIFGYIAGM
                     GIAVAVAPCITPLLTSTLKMPTLQTWLVIVAIFVAAGAVLSALLRLALNEMVGPNISV
                     PDRIAGAALGALRIALLAILLVVVFDRAIPPGQEPAFLKGSQWRPVLSSAAERGLHHL
                     PPDVEAYIDRLKHYRRVL"
BASE COUNT          952 a         1623 c         1671 g          993 t
ORIGIN      
        1 ccgcgcctgt ttcaggacgg caggtgtccg aggacgcgct gcaggattgt cgaatgcggg
       61 cggccacctc ttgcggattt ccgcgcccgc gaggcgccgc gatgagggtg gcctttcgaa
      121 gcgcggcggc gcgattttga cttgcggccg gggctgcgag tcttgcggcg cgaacgcagg
      181 atgtggcggc ccacactgct gaggaacaac ctctccaggg ggtagctcaa cttccggacc
      241 gccgccgata gcggcagcca ttcgactgcc atgatgtcct ccatgacatc acggcttgga
      301 ttgacctcgg cctgcatgcg ccagaactgc acgaccttcg ggcgcccgcg cgcccggtag
      361 gtaatcgcgc ccacaaattc gcgcacgcga acgcggtgtc cggtttcctc gacgacttcc
      421 cggcgtgcgc cggcgagcgg actttcgtca cgcttgagct tgccgcgcgg cagcacccag
      481 agctcgtctt tcgcccgctg cacgatggcg atcagcgggc gcgcgccgtt gcgcaccacg
      541 atgccgcctg ctgcatgaat ggtcttgccc gccatggcgt tcgacgtgta gcaaaaaaat
      601 gcggcgatac acgcatcgaa atgcgaatgc gcgcgttcct gtgggcaagt ttcgctcgat
      661 ggactgctgc gctgacgcgc ggcgtcagtt gaatttcaac ccggcttcct tggcgatctt
      721 gaggttgagc gcgatctcgc ggcggatcat ggcgtccatc tcggcgggcg tcagcggcgc
      781 cggctcgacc ccctgtatct tcagtttctg ctgcacgtcc ggaagcgtca gcacctttgt
      841 cgtctccgta taaagacggt cgacgatcgg ccgcggcgtc ttcgcgggga ccagcatgcc
      901 gttccaatag ttgaaatcgg aatctttgta cccggcctcc aacgtggtcg gcacatcggg
      961 cagggtcggc gagcgtttgg ccgagctcac cgcgagcggc acgagctgct tgttctggat
     1021 gaagggcagt ccggacgaaa tgccgatgaa gatgtagtcg acccggcctg tcatcacttc
     1081 ggtgagcgct tccggcccgc cacggaatgg cacgtgggtg accttgagtc ccgcgctcac
     1141 gttgaatcgc tccgctgccc aatgggtggc gctgccgact cccgccgatg cgaagctcag
     1201 actgccggcc ttggccttcg ccaccagttc ctgcaccgtc ctgatgccct ttgacggcgc
     1261 aaccagcagc acattcggca cgttgccgaa gcttgccacc gccgagaaat cattggccgc
     1321 gtcgtagggg aggttcgggt aggcggccgg cgcgcccgaa tgcgcggcgg cgttgatcag
     1381 gatggtatag ccgtcggggt cggccttcgc gaccgccgcc gatcccagac tgccgccggc
     1441 gccgccgcgg ttctcgacca cgattgtctg gccgagctgc ttcgacagcg gatcgagcac
     1501 gatgcgtccg aggatgtcga tggtgctgcc ggccgagaag ggtacgatgg cgcggatgga
     1561 tcgcgcagtc ggccaggttg tctgtgccga tgccggaccg gacagcatcg gagtcgccag
     1621 catgccggcg gtgaactgca agaatttacg gcgatcgtgc ccagtcatgg tgtctccccc
     1681 gatgatgtgt tcggcaccca ctatggcgac gacttgtcta caggtttggt cactttgatt
     1741 tcgctgatcg caggtttgct cttcgatttc tttcgcacgg tgatggtgcc ctccttgata
     1801 tgctctcctt tcaccgtgtg cttcggcgtt gatgacttct ggccctttgc cagcgccggc
     1861 ggcgacgcaa ccgcaagcac tgcagccagt gcaatgacga gcccggtatc agatttcagc
     1921 atggcgaaac tttctggatg gtggcgcccc tgaataaact ctagcaccgt cttctcccaa
     1981 tgcgcgagcc ttcgcccaca aggctgttgc gccagcccgt tcgcggcgca taggctccgc
     2041 gccgccacac acggagaata cgaatggcag atttcaagtg ggatcacgtt catctgcgga
     2101 cgccggaccc cgaagccact gcgcaatggt tcgagcgcat gttcggcgcc gaggtgatcc
     2161 gctcgacgca gcagggcaag ccgcgcgtgg acatcaggct tggcggcgcc atgattttca
     2221 ccgcgcaggt caattccggt gacggcgtaa accccgcacc ggtgacgccc tatcagggcc
     2281 tcgaccactt tggcctcgcg gtttcgggca tcgacgcgat cgcggccgat ctcaggaaga
     2341 agggcgttga attcaccaag gaaccacatt cgccgcggcc cgggatcaag gtctgtttcc
     2401 tgcgcgggcc gcaaggtatt tcgatcgagt tgctcgatcg cgatccgaaa tactcctgac
     2461 gccgcgctgg aaaattgaag gaagcggttg ctctcggcct cctgtcgcgc ctaaatggcg
     2521 ccggggcgct gcggcagagg ggaacaatac gaatgcgtgc gttcggactg gcggtggtcg
     2581 ccgcaacgat ctcggcggca accgcgacgg ctgccgatgg gcggttcgaa cgcaccttga
     2641 agatgctggc tccggaggag cggctcgagc agctttgcga ttacaccgcg atgatgcgca
     2701 ttcgcaacga gcaaaagcaa tttcgtcccg atcgcgcggt cgccaatgcc atgaccgaac
     2761 cgaaggccac gggcaatacg ctcgaagtca ccggcggcgc cttccgcagc cgcatgaaat
     2821 ggtatgcgct gtcttaccgc tgcacggcga cgcctgaccg tatgagcgtg gtggcattcc
     2881 atttcacgat cggcgccgag atccccgagg cgaaatggtc gagctatggc ctgtgggact
     2941 gacggcgctg ctattggtgc tgcacaacca cctttgtccc gataggtacg cggtcgtaaa
     3001 gatccatcac atcttcattg accagccgga agcagcctga cgaaaccgcc tggccgatcg
     3061 tttccggcgc gttggtgccg tggatgcgat agacggtcgc gccgaggttc atcgcgcgtg
     3121 cgcccagcgg attgcccgga ccaccggcca tccagcgcgg caaatacggt tgccgctgaa
     3181 tcatctccgg cggtggcacc cagtccggcc attcacgctt gagcgtgatc ttctggatgc
     3241 cgccccactg gaatccgtcg cggccgacgc cgatgccgta gcgcagcgcc actccattgc
     3301 ccatgatcag ataaaggtaa cggtctgcgg tgttgacgat gatcgtgccc accggttgct
     3361 cggtccgata gaagaccgac ttgcggcgaa actccggcga cacgtcgaca tcctttgcgg
     3421 tgtggtcgac tcccggatcg acatcctgca tcgtaggcgt ggatgatggc agcagagttt
     3481 gcgacattgc cggcgcggcc ggtaccagaa gaatcgcgag cgcgaaaaca cgcaaccgaa
     3541 gcatttcaaa tcccccgaat agtgcgccgg tggcgcgtca gtcttagtct ttagtctttg
     3601 tctttaatct gtgtgagtgg ccgatcggag ccgcgtgccg tctcggtgtg ccagcggccc
     3661 ttttcgtctt cgtattctat ggtttccgta tggccgggaa cggtctgctc tgcggcagcc
     3721 cgctgcgccg ccgcgagcgc ggccgcatgc gacgggtaag gttccgaaaa cacgccgtcg
     3781 accttgtagg cccagccgcc gtcgtgctcg acgatctcgt atgtgacctc caccatgaac
     3841 gacccctgca gcacgcagaa cctgccgggt aacgtagcgc caatggggct ggttgcgcca
     3901 gtcacaaggc tcgatgggtt gcaatagtta ccgccatgcc agctatgcaa gcggcctgac
     3961 tgccgtcaaa aatcgaattc gagcccaaag cggcgcctaa acgccgtgcg atcgtgcggc
     4021 agggctgcct tgagaatcgc gcgcgcggaa aagttgccct ttattgcgcc tgcctccgtg
     4081 tacgcccagt cgacgatgtc acgcctgctg aaagcgatgg tgtcgccctt ttttggcccg
     4141 gcgagcgtga gcggcgaact attgacctgt ccgatgaacc gttcgccgac atgcgcgaac
     4201 ggatagatcc agaagagttc cggaccgccg tcgccgacca atgcgatctt gacggcaaag
     4261 tgctccatgg ccggtcccgg atgtctggcg agcgcaagaa agccggagag ggcttcacgc
     4321 gcgttctgca cggccgccga catggcccga tcgttgagcg cgagaaagga cagggcgctt
     4381 tcgccggcct ttgccggctc cggttccggc ggtgatgccg tcaggccggc tgcgcgcgcg
     4441 gcgttgagaa tctgcgcaaa gcctgtcgcg gttttcttcg ctgcgttttt cgacgccatg
     4501 gcacggcttt ttcaccggca tcgcaggcaa gcgttccggt ccggtcatgg gtcgcgtggg
     4561 caacgaaaag gttcaaagcg cgccggcctg cagcgtacga gccgccgcta caacacgcgg
     4621 cggtagtgct tcaggcggtc gatataggct tcgacgtccg gcgggaggtg atgcaggccg
     4681 cgctccgcgg ctgaggacaa aaccggccgc cattgcgagc ctttcaggaa tgccggctcc
     4741 tggcccggtg gaatggcgcg atcgaaaaca acgaccagca ggatcgcgag gagcgcgatg
     4801 cggagcgcgc cgagtgccgc gcccgcgatg cgatcgggaa cgctgatatt gggaccgacc
     4861 atttcgttga gggcaagccg cagcaacgcg ctgagcacgg cacccgcggc gacgaagatc
     4921 gcaacgatca cgagccaggt ctgcagcgtc ggcattttaa gggtgctcgt aagcagcggc
     4981 gtgatgcacg gcgccaccgc cactgcgata cccatcccgg cgatatatcc gaaaatcgtc
     5041 gccaggctgc gcagcaggcc ggtcatgaag cccatgacaa cggcggcaaa caggcagacg
     5101 tagatcgcaa ggtcgaacca gttggtcgag aaaaaagagg tcattgcgaa ttgttcggac
     5161 gggactaggc tgcggacgtt tgaagacctg aaagaatcga ttcaaggaga cggtacatgg
     5221 cgttcacggt cacgagcaa
//
LOCUS       DASSRL010000112         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1350146, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000112 DASSRL010000000
VERSION     DASSRL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350146"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFL49_02400"
     CDS             <1..1060
                     /locus_tag="VFL49_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910856.1"
                     /translation="TSAGALCALMAWYGLAPKKGSPNSGTAPEAIKKLEDFWQDFVAR
                     TGAENLLNLFTFGAFRAEEIEVPLLGLSARVFGVNPYGAGYNAFAAFLPSLGVRKQYF
                     DLDELLAETCPALKDNSIDWPKVETRLLVGASEVVNGLETVFDSGINKGVQGANKGRQ
                     PVKNLKDKARYWRQQLPLSLEGVSASGTLPALREAEHISDGHYWDGLYSQNPPVREFI
                     AGPETENIPDELWVVRINPQQWPYVPRTNKDIQDRQNELMGNLSLNKELDFIMSVNAW
                     NNEYANENFGKDHKNVTVRTIMMEKAIADDLSYSSKFDRSRAFMDRMCQEGHKVARAW
                     LDRWKANAAKEYPEDAAF"
     gene            1232..4615
                     /locus_tag="VFL49_02405"
     CDS             1232..4615
                     /locus_tag="VFL49_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7910857.1"
                     /translation="MVAAMDVAGWLQNLGLGQYASAFAENAIDIDILPELSEHDLEKL
                     GIPLGHRKRLVRAIRGMAGAGAASKPITGERGEMAPSGHPPKGAAERRHLTVMICDLV
                     GSTALSARLDPEDMGAVIDAFQATCARITLAYDGFLADFRGDGILAYFGYPRAHEDDA
                     ERTVRAALDIAAAVARVKTPADEPLSVRIGIATGLVVVGDLGGEGTLGEHAVVGDTPS
                     LAARLQALVEPGTVVVAAATRRLLGDLFHLRDLGLHKVKGLAEPVAAWVVDGVAASKS
                     RFEAIHAGGLTDLIGREEQLDFLLKRQQLAWKGKGQVVLISGEPGIGKSRLVRALEQR
                     IADEPHTLLHYQCSPHHTNSALYPFIAQFERAAGFKTDDTPEKRLDKLEALLARAAPR
                     IQDTAPFFAALLSIPFGNRYPPLAPNRTQQRRRTFAALFDQFESLARQRPILLLFEDA
                     QWADATSLELLDLTIERIRQLPVLALFTMRPDFESPWVGLPNVGTLALGRLDRNNVES
                     IVTQVAGGHALPAEVMNQIVVKTDGNPLFVEELTKAVLEGDILVKDAGGYRLTGPLPP
                     LAIPTTLQDSLMARLDRLAPVKEIGQIGAAIGREFSYSLMREVVGGDEPSLKSALAKL
                     EQAELVFRRGDPPEAIYSFKHALVRDAAYESLLKSRRQRLHGQIARTMEEKFPDIVMT
                     QPEIVAHHFTEAGFAEPAINYWLKAGNLALSRSANAAVSHLQQGLKLIPGIEDPTLRN
                     KSELLLQTSLGNSLRATTGWSSDSVRHAYTRALHLCRESGLDEHTFPAVFGLWTWNFL
                     RASLREAQALAEYLLNTAERVADSAYEVLAREALGFTLFAQGQFAAAHTELERGISLC
                     EDSKAAAYLDLSAQDPRVHVRSYDSMTLWFCGFPDQALRLCAEARRYADVSRHPFSEA
                     MARTISLRVHQLRGEASVVAGQANAAIAFCEEHEFVHYLAMAVILRGWARALQGEFED
                     GIAEIREGLEKERAIGALLFDSYSLGLLADACIRNKQYGQALEFLEQAQSRLEEENSS
                     HFYAAEIFRLLGETYLRSNKDLDQAEHYFSKGLKVAREQKARSLELRLCLSMCDLHDS
                     GQSAGKCRAQLGAVYGTFTEGFDTADLVRAKARLERLEK"
     gene            complement(4628..5026)
                     /locus_tag="VFL49_02410"
     CDS             complement(4628..5026)
                     /locus_tag="VFL49_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HET7910858.1"
                     /translation="MMLHRSFDVRLPPFAQADEIFADIIRQFDTPAANRMLEIPKLRA
                     VLLPTIRAEFRMAYNYEHAPAERFSFPISSFVGDSDPWVSQEDSAGWGEFTRGGFTNH
                     VRKGSHFLMAEDGGYILETINSEFVDSAIK"
BASE COUNT         1046 a         1452 c         1542 g         1015 t
ORIGIN      
        1 gacgtcggcc ggcgccctgt gcgcgttgat ggcctggtac ggccttgctc cgaagaaggg
       61 aagccctaac tccggcacgg ccccagaagc gatcaaaaag cttgaagact tctggcagga
      121 cttcgttgcc aggacgggcg ccgagaattt gctgaactta tttacattcg gggcattcag
      181 ggcggaagag atagaggtac cgctgcttgg attaagtgcg agagtttttg gcgtcaatcc
      241 ttacggcgca ggctacaacg cgttcgccgc ctttctgccg agtcttggcg tgcgcaagca
      301 gtattttgat ttggacgaat tgcttgccga gacgtgtccg gcgttgaagg acaacagtat
      361 tgactggccg aaggtggaga ctcgcctgct ggtcggcgcc tcggaggtcg tcaacgggct
      421 tgaaactgtc ttcgactccg gcatcaacaa gggagtgcaa ggcgcgaata agggacggca
      481 gcccgtcaaa aatctcaaag acaaggcgcg ttactggcgc cagcaattgc cgctcagcct
      541 ggagggagtt tccgcgtcgg ggactttgcc tgctctccgc gaggcggagc acatcagcga
      601 cggccattac tgggacggtc tgtactcgca gaatccgccc gtgcgcgagt tcattgctgg
      661 acccgagacg gagaatatcc ccgacgaact gtgggtcgtg cgcatcaatc cgcagcaatg
      721 gccgtatgtg cctcgaacca acaaggacat tcaggaccgt cagaacgagc tgatgggaaa
      781 cctgtcgctg aacaaagaac tggatttcat tatgtcggtc aacgcatgga acaacgaata
      841 cgcgaacgaa aattttggga aggaccacaa aaatgtgacg gtgcgcacga tcatgatgga
      901 aaaagcgatc gcagacgacc tgtcgtattc atcgaagttt gaccgcagcc gcgccttcat
      961 ggaccggatg tgtcaggaag gccacaaggt cgcccgggcc tggctcgacc gctggaaagc
     1021 gaatgccgcg aaagaatatc cggaagacgc cgccttctga aattttgaga aggtaatgcg
     1081 atgacgcgaa ttcgttgcgc cgtcaggtgg tgcagcggta gcataatctt atagtggcgc
     1141 gagcgcagtt ctgcacccgg cagcgaatgc tggcgctctc aggccgtgca attgggctgg
     1201 tcgcaccccg cccatgattc tggaaaggta tatggttgcc gccatggatg ttgccggctg
     1261 gctgcaaaat ctcggactcg gacaatatgc ttcggcattc gccgagaacg ccatcgacat
     1321 cgatatcctg cccgagttga gcgaacacga tctcgaaaag cttgggatcc ccctgggcca
     1381 ccgaaagcgt ctcgtcaggg cgatcagggg gatggcggga gccggcgccg caagtaagcc
     1441 cataacgggc gaacgcgggg agatggcgcc gagcggacac ccccccaagg gcgccgccga
     1501 gcgccggcac ctgaccgtga tgatctgcga cctggtgggc tcgactgctc tatcggcccg
     1561 actcgacccg gaagacatgg gcgccgtgat cgatgcgttt caggccacct gcgcccgtat
     1621 cacgctggcc tatgatggct tcctcgcgga tttccgcggc gacgggatat tggcctattt
     1681 cggttacccc cgtgcgcacg aggacgatgc cgagcgaacc gtgcgtgcgg cgctcgatat
     1741 cgctgctgct gttgcacggg tgaagacgcc tgcggacgag ccgctctcgg tccgtattgg
     1801 gatcgccacc ggtctggtcg tggtcggcga cctcggtggc gaggggacgt taggagagca
     1861 tgccgtggtt ggcgacacgc caagtctggc ggcacgcctg caggccttgg tcgaacctgg
     1921 gaccgtggtc gtcgccgctg ccacgcgccg gttgctcggc gatctgttcc atctgcgcga
     1981 tctcggcctt cacaaagtca aaggtctggc cgagccggtc gccgcgtggg tggttgacgg
     2041 cgtggcggca tccaagagcc gctttgaagc gatccacgcc gggggcctga ctgatctgat
     2101 cggccgcgaa gaacagctcg atttccttct gaaacgccaa cagctcgcct ggaaaggcaa
     2161 aggccaggtc gtcctgattt cgggcgagcc ggggatcggc aagtcgcgtc tggtgagggc
     2221 cctcgaacag cgcattgcgg acgagccgca tacgctgctg cattaccaat gttcgcctca
     2281 ccacaccaac agtgccctct atccgtttat tgcccagttt gagcgcgcag ccgggttcaa
     2341 gacggacgat acgcccgaga agcgcctgga caagctggaa gcgctcctcg ccagagccgc
     2401 accgcgaatt caggatacgg caccgttttt tgccgcgttg ctgtcgatcc cgtttggcaa
     2461 cagatatccg ccactggcgc ccaaccgaac acagcagcgc cgccgcacgt tcgccgcctt
     2521 gttcgatcaa ttcgagagtc tcgcgcgcca gcgtccgatt cttcttctgt tcgaggatgc
     2581 gcagtgggcc gatgccacct ccctggaact gctcgacctg acgatcgagc gtattcgcca
     2641 attgccggtc cttgcgcttt tcacgatgcg accggatttc gagtcgccct gggtcggtct
     2701 ccccaacgtc ggcacattgg cgctcggtcg cctcgaccgc aataacgtcg aaagcatcgt
     2761 cacgcaggtg gccggcggcc acgctttgcc ggctgaagtg atgaaccaga ttgtcgtcaa
     2821 gaccgatggc aatccgttgt tcgtcgagga gctcaccaag gccgtgctcg aaggggacat
     2881 cctggtcaag gacgccggcg gctatcggct tactggccct ctgcctccgc tcgccattcc
     2941 gaccaccctt caggattccc tgatggcgcg gctcgatcgc ctggcgccgg tgaaggagat
     3001 cggccaaata ggagcagcca ttgggcgtga gttctcctac tcactgatgc gcgaggtggt
     3061 cggaggcgac gaaccgtcgc tgaaaagcgc tctcgccaaa ctggagcagg ccgaacttgt
     3121 gttccgccga ggtgacccgc cggaagccat ctacagcttc aagcacgcgc tggtgcggga
     3181 tgcggcatat gagagtctgc tcaagagccg ccggcagcga ttgcacggcc agatcgcgcg
     3241 cacaatggag gaaaagtttc ccgacattgt gatgacccag ccggaaatcg tggctcacca
     3301 ctttaccgaa gccggctttg ctgagcccgc aatcaattac tggcttaagg ccggcaatct
     3361 tgcgctcagt cgttcagcga atgccgccgt cagccacctg caacaagggt tgaagctcat
     3421 tcccggtatc gaagatccga cgttgcgcaa caagtccgag ttgctgcttc agacgtcact
     3481 cggcaattcg ctgagggcaa ccacgggctg gagctctgac agcgtgagac atgcgtatac
     3541 gcgggcactc catttgtgca gggagagcgg gcttgatgaa catacctttc ccgccgtgtt
     3601 tggtttgtgg acgtggaatt tcctgcgcgc gtcccttcgc gaggctcaag ccctcgccga
     3661 atatttgctg aatactgccg aacgtgtggc cgactccgct tacgaggttc tcgcccgcga
     3721 agcgctggga tttacgctgt ttgcacaagg ccagtttgcc gctgcccaca ctgaactgga
     3781 gcgcggcatc agcctctgtg aggacagcaa ggctgcggcg tatcttgacc tgtcagcgca
     3841 ggacccgcgc gttcatgtaa gatcatatga ctccatgaca ctttggtttt gcggctttcc
     3901 ggatcaggct ttacggcttt gcgcggaggc ccgtcgttac gcggatgtaa gccgacaccc
     3961 gttcagcgag gcaatggcgc gaacgataag cctccgggtg catcagcttc gcggtgaggc
     4021 gtcagtcgtt gcaggtcagg ccaatgcggc aatcgcattc tgcgaggagc atgagtttgt
     4081 acattacctc gccatggccg tgattttgcg tggatgggca agagccctgc aaggcgagtt
     4141 cgaggacggt attgccgaga ttcgcgaagg tttggaaaag gaacgggcga tcggcgcctt
     4201 gttatttgac tcgtactcgc tgggattgct ggccgacgca tgcataagga acaaacaata
     4261 cgggcaagcg ctggaatttc tggagcaggc gcaatcgagg ctcgaagaag aaaactcctc
     4321 gcacttttac gcggctgaga tattccgctt gctgggcgaa acatatttgc gatcgaacaa
     4381 agatttggat caggcggagc attatttttc caaaggcctt aaggtcgcac gcgaacagaa
     4441 agccagatcc cttgaactca ggctttgcct gagcatgtgc gacttgcacg attccggaca
     4501 aagcgccggt aagtgtcgcg cgcaactcgg cgcggtctac ggcactttta ccgaaggctt
     4561 tgacactgcg gacctcgtca gagccaaggc aaggctggaa cggctcgaga aatagtgtcg
     4621 gtcgcgttta tttaattgct gaatcaacga actcactatt gattgtctcc aggatgtacc
     4681 cgccgtcctc ggccatcaga aaatgtgaac ctttgcgcac atgattggtg aacccgccgc
     4741 gagtgaattc gccccagccg gcggagtctt cttgcgaaac ccacgggtcc gaatcaccga
     4801 cgaaactgct gatcggaaag gaaaagcgct cggccggtgc atgctcgtaa ttataggcca
     4861 tgcggaactc ggcacggatc gtgggcaaca acaccgcccg cagcttcggt atctccagca
     4921 tcctgttggc agccggagta tcgaattggc gtatgatatc cgcgaagatt tcgtcggcct
     4981 gggcgaaagg cggaagcctg acgtcaaaac tccgatgcag catcatgtcg taagccaggt
     5041 tgtcctcaaa ctctc
//
LOCUS       DASSRL010000113         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_352682, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000113 DASSRL010000000
VERSION     DASSRL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352682"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFL49_02415"
     CDS             complement(<1..184)
                     /locus_tag="VFL49_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910859.1"
                     /translation="MVALIAATLPFAKWGQSQIEWVKLGRMDGSDRKSGVRTRPRLGR
                     GLRVIVIAVLAVVLLPY"
     gene            183..1112
                     /locus_tag="VFL49_02420"
     CDS             183..1112
                     /locus_tag="VFL49_02420"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET7910860.1"
                     /translation="MKSDTDHFADRLTMTARDIETLLNRLLGPAALSSETARPPRLVA
                     AMRHAALDGGKRLRPFLVVETAALFGVDRVGALMAGAALELIHCYSLVHDDLPAMDND
                     NLRRGRPTVHKAFDDATAILAGDALLTLAFDVMARTEVHPDAAVRAALVLELARASGL
                     GGMAGGQMLDLAAEGRFENRRTLSENEIITLQAMKTGALIRFACNAGAILGKADAAAR
                     ASIAQYGSAIGQAFQIADDLLDVEGDAKTLGKAAGKDAAAGKATLVATLGVDKARARL
                     NELVADAGSALGSFGEKSATLRAAARFIAERRA"
     gene            1128..1805
                     /locus_tag="VFL49_02425"
     CDS             1128..1805
                     /locus_tag="VFL49_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HET7910861.1"
                     /translation="MSRRTRDPLMPVRLARLHARLLISVAVGLAVTLALASTDWRLAT
                     RLLAGWDVGVGLYLIFAHLLMSRATVSEIRRRAAIQDEGAAALLVLSPAAAIASLAAI
                     VSELGNAQLREGWADLALGMGTILLSWLFLHTVFALHYAHEYYGQGSDDQVGGLKFPG
                     SRDPDYWDFVYFSLVIAMTSQVSDVAITSKAIRRVATTHGVLSFFFNLTVLALTVNMV
                     SNLIKPG"
     gene            1812..3419
                     /locus_tag="VFL49_02430"
     CDS             1812..3419
                     /locus_tag="VFL49_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET7910862.1"
                     /translation="MAGTMETLLLMLAVLAAAAVIAQRLGTPPSIILVIAGIVMALIP
                     GLPRIDLAPEVVLLVILPPLIYSAGVAMSWREFRFNLRPITLLAFGCVIFTTCAVAAV
                     THYFLKFEWAIGFLLGAIVAPPDVVAPLAIARRLGLPRRILVVLEGEGLANDATALIL
                     YKFAVAAAVTGAFSLPKAGATFGMIVVGEIFHGVVVGWLSLRLRQWAREPRVEITLSL
                     MTPYLAFWVPEHLGGSGVLATVACGLYVSWNGPRLISSATRLQGIFFWDLLVYLVEGL
                     VFLLTGLQANALFDQVKAFSMRELAVAVVWTTVVVVLARFVWVFPATYIPRWVSPSLA
                     KRDPSPPWQNPFMISFIGVRGVVSLAAALAIPYALNNGQPFPHRELILFISFGVIIFT
                     LVGQGLMLPLVVRCLGIARSGEEERSSQIESELGVRLAALEAVEKRLEKFIQERELPD
                     EVIALLRTRNQTRRQILPSNMADGLDHMRQSAALKKELIDAERDFIYQLLREGKITDE
                     ARRRIEYELDLEEAAVANRGRDGGGWM"
     gene            complement(3425..>3966)
                     /locus_tag="VFL49_02435"
     CDS             complement(3425..>3966)
                     /locus_tag="VFL49_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7910863.1"
                     /translation="IVLPADRFGAAQWLGLGLSFAGMLIAFGLPTPALDPRQHIGDAM
                     MVGGALFWAATTLTIKASPLTRASAEKVMLYQLVVSGPIMAVAAFFAGERMIHMPSAL
                     AIGSMVYQTVWVVSVTFVIWFALIARYSATRLSAFTFLTPLFGVAAGHLVLDEPLTPA
                     FSAAVALVAAGLVLVNRSR"
BASE COUNT          729 a         1207 c         1196 g          834 t
ORIGIN      
        1 cataaggaag cagcacgacc gccagaaccg cgatcacaat gacacgcagc ccgcggccta
       61 atcgcggtct agtccgcacg cccgattttc tgtccgaccc gtccatccgt cccaacttaa
      121 cccattcgat ctgcgattga ccccactttg cgaaagggag cgtagccgct atcaatgcga
      181 ccatgaaaag cgataccgac catttcgcgg accggctcac aatgactgcg cgcgacattg
      241 agacgctgct caaccggctt ctgggtccag cagcgctgag cagcgagacg gcgcggccgc
      301 cgcgcctggt cgccgccatg cgccatgccg cattggacgg aggaaagcga ctgcgccctt
      361 tcctggtggt ggagacggcg gctctcttcg gcgttgaccg cgtcggcgcg ttgatggcgg
      421 gcgcagcact tgaactcatc cattgttatt cgctggtgca tgacgatttg ccggcgatgg
      481 acaacgacaa cctgcgtcgc ggaaggccga cggtacacaa ggcgttcgat gatgcgactg
      541 caattcttgc tggtgacgca ctgctgacgc tcgcttttga tgtcatggcg cgcacggaag
      601 tgcatcccga cgccgctgtg cgcgcggccc tcgtacttga acttgcgcgg gcatccggac
      661 ttggtggcat ggcgggcggc cagatgctcg acttggcggc cgaaggccgc tttgaaaaca
      721 ggcgaacgct cagcgaaaac gagatcatca cgttgcaggc catgaagacc ggcgccctga
      781 tccgttttgc ctgcaatgcc ggcgcaattc ttggaaaggc cgacgccgca gcacgcgcga
      841 gcattgcgca atacggttcc gcgatcggcc aggcattcca gatcgccgac gatttgctcg
      901 acgttgaagg cgatgccaag accctgggca aggcagccgg caaagatgca gcagccggca
      961 aagcaacgct cgttgcgact ttaggtgtcg acaaggcgcg cgcccggctc aatgagctgg
     1021 ttgcagacgc cggctcggcc ttgggctcat tcggtgaaaa atcagctaca ctgcgcgctg
     1081 ccgctcgctt catcgcggaa aggcgagcct gagctggccg aggactgatg agccgcagga
     1141 cacgtgatcc gttgatgccg gtgcgtctcg cgcgcttgca cgcgcgattg ctgatctccg
     1201 tcgcggtcgg ccttgccgtc acgctggcgc tggcatccac agattggcgc ttggcgaccc
     1261 ggctgctcgc cggatgggat gttggcgtcg gcctctatct catcttcgcc cacctgttga
     1321 tgtcacgtgc aacggttagc gaaattcgcc gtcgcgccgc cattcaggac gaaggcgccg
     1381 ccgcgctact ggtgcttagt ccggcggctg cgattgcgag cctcgccgcc atcgtttcgg
     1441 aacttggcaa tgcgcaatta cgagaaggct gggccgatct tgccctcggg atgggcacca
     1501 tcctgctgtc ttggctgttc ctgcacactg tctttgccct gcactatgcg cacgaatatt
     1561 acggccaagg cagcgacgat caggtcggtg gtctcaaatt tcctggatcg cgagacccgg
     1621 attattggga ttttgtctac ttctctctcg ttattgcaat gacctcgcag gtatcggacg
     1681 tggcaatcac cagcaaagcg atccgtcgcg tcgccaccac gcacggcgtt ttgtcgttct
     1741 tctttaacct cacggtcctg gcactgaccg tgaacatggt ttccaatttg atcaagccgg
     1801 gctaaagcgc catggccggc acgatggaaa cgctgcttct gatgcttgcg gtgctcgctg
     1861 ccgcagcagt gatcgcacag cggctgggga ctcctccgtc aatcatcctc gtgatcgccg
     1921 gcatcgtgat ggcgctgata ccgggattgc cgaggatcga tctcgcgccg gaagtcgtgt
     1981 tgctggtaat cctgccgccg ctcatttatt cggccggcgt cgccatgagc tggcgcgagt
     2041 tccgcttcaa tctccgcccg attaccctgc ttgcgttcgg ttgcgtcata ttcacgacat
     2101 gcgctgttgc ggccgtaacg cattattttc tgaaattcga gtgggcgatc ggcttcctgc
     2161 ttggagccat cgttgcgcca cccgacgtgg tggcaccttt ggccatcgcg cgccgcctgg
     2221 gcctgccgcg acggattctt gtcgtcctcg aaggcgaagg gctcgcgaac gacgcaaccg
     2281 cgcttattct ctacaaattt gccgtcgcag cggcggtaac tggcgcgttt tctctgccta
     2341 aagccggtgc cactttcggg atgatcgtgg ttggggagat tttccatggc gttgttgtcg
     2401 gttggctgag cctgcgtttg cggcaatggg cgcgcgagcc gcgcgttgaa atcacactgt
     2461 cattgatgac gccctatctc gcattctggg tgcctgaaca tctcggcgga tcaggagtgc
     2521 tcgccaccgt tgcttgcggc ctttacgtaa gctggaacgg cccgcggctt atttcctcgg
     2581 cgacacggct gcagggcatc ttcttctggg atctgcttgt ctacctggtc gaaggccttg
     2641 tgtttctgct gactggccta caggccaacg ccctgttcga ccaggtaaag gcattttcga
     2701 tgcgcgaact ggcggtcgcc gtcgtctgga cgaccgtggt cgttgttctt gcgcgttttg
     2761 tctgggtatt ccctgctaca tacattccac gctgggtcag cccgtcgctg gccaagcgcg
     2821 atccgtcgcc gccgtggcaa aaccccttca tgatttcctt cattggcgtg cgcggcgtcg
     2881 tatccctggc cgccgccctc gccattcctt atgcgctgaa caacggtcag ccgtttcctc
     2941 atcgtgaact gatcctgttc atttctttcg gtgtgatcat cttcaccctt gtcggccagg
     3001 ggctgatgct gcctttggtc gtacgctgtc ttgggattgc tcgctccggc gaggaagagc
     3061 gatcgagcca gattgaatcg gaacttggag tgcgcctggc ggcgctggaa gcggtggaaa
     3121 aacgcctgga aaaattcatc caggagcgtg aattgccgga cgaagtcata gcgttgcttc
     3181 gcacgcgcaa ccagacccgc agacagattc tgcccagcaa tatggcagac ggtctcgatc
     3241 acatgcgtca gagtgctgcg ctgaagaaag agctgatcga tgccgaacgc gatttcatct
     3301 atcagctgtt gcgcgagggg aagattaccg acgaggcgcg acgccgcatc gaatacgagc
     3361 tcgatctcga ggaggcggcc gtcgccaatc gcggccgtga cggcggcggc tggatgtgat
     3421 gacctcagcg tgatctgttc accagcacga ggccggcggc gaccagtgcg acggccgcac
     3481 tgaatgcagg cgttagtggt tcatccagca cgagatgtcc ggcggccacg ccgaacagcg
     3541 gcgtaaggaa cgtaaacgcc gaaagccgtg tcgccgaata gcgcgcaatc agtgcgaacc
     3601 agatgacgaa cgtcacgctg accacccata cggtttggta gaccatcgat ccaatcgcca
     3661 gcgccgatgg catatggatc atccgctcgc cggcaaagaa tgcagcgacc gccatgatcg
     3721 ggcccgatac gaccaactga tagagcatca ctttttcagc ggacgctcgc gtcaacgggc
     3781 tggctttgat ggtcagcgtg gtcgccgccc agaacagcgc gccacctacc atcattgcat
     3841 caccgatatg ctggcgcgga tcgagtgccg gcgtcggcag accgaacgcg atcagcatgc
     3901 cggcgaatga caagccgaga cccagccatt gcgcagcgcc gaaacgatcg gccggaagaa
     3961 cgatgc
//
LOCUS       DASSRL010000114         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_125605, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000114 DASSRL010000000
VERSION     DASSRL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125605"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFL49_02440"
     CDS             complement(<1..724)
                     /locus_tag="VFL49_02440"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7910864.1"
                     /translation="MRTRLTERLGIEHPVISAPMGFVAGGRLAAAVTAAGGLGLIGGG
                     YGDAEWLEREFASAGNTRVGCGFITWSLAQQPQLLDKVLTHAPAAIMLSFGVPALFAQ
                     PIKAAGALLVCQIQSMAHAREAVAAGADIVVAQGTEAGGHAQTRTTFTLVPEVADYLV
                     KAAPDTLLVAAGGVGDGRGLAAALMLGADGVLLGSRLLMTEEALVPPGFHDAIIRADG
                     DATIKTRVIDVVRGLNWPTEITG"
     gene            complement(736..1596)
                     /locus_tag="VFL49_02445"
     CDS             complement(736..1596)
                     /locus_tag="VFL49_02445"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET7910865.1"
                     /translation="MPGQSWSKTWTFYEGGWREGNVAIMGVRDHAAWLCSSVFDGARA
                     FEGVSPDLDLHCARVNASAETMHLKPVVSADQWLDLTREGVKRFDKDAALYIRPMYWA
                     ERSGTLLVAPDPESTKWCLSLYEAPMRKPEGFSVTLSPYRKPSLECMPVNAKAGCLYP
                     NNARALLEANTRGFGNCVVLDLLGNVAELATANIFIGKGGVVFTPAPNGTFLNGITRQ
                     RVIKLLREAGVDVIETILSYRDVQNADEIFSSGNYSKVMPVNRIDERSLQPGPLYRKA
                     RELYWDFAHG"
     gene            1764..2420
                     /locus_tag="VFL49_02450"
     CDS             1764..2420
                     /locus_tag="VFL49_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET7910866.1"
                     /translation="MSIFVFDAYGTLFDVHAAIARHRQEMGPDADRISAVWRTKQLEY
                     SWTLTLAGHYTDFWTLTERALDYALALVPDAKKTLRPKLLDAYFKLDAFPDARSTLQS
                     LKAKGRKTGILSNGSPAMLKGAVDAAGIGSNLDALLSVDALKMFKPRPEVYGLVTRHF
                     GCKPGDVIFVSSNRWDVMAGVSVGFRSMWINRAKMPDEYTDFAPAQVLADLNALTDFV
                     "
     gene            2505..>3208
                     /locus_tag="VFL49_02455"
     CDS             2505..>3208
                     /locus_tag="VFL49_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910867.1"
                     /translation="MQPIISVKGLSKSYASGFKALSGIDLDIRRGEIFALLGPNGAGK
                     TTLIGIICGILVPTQGKVTVDGHDIIKDYRAARSLIGLVPQELHTDAFESVWATVSFS
                     RGLFNKPKNPDYVEKVLRALSLWDKKDNNIITLSGGMKRRVLIAKALSHEPQILFLDE
                     PTAGVDVELRKDMWQVVRDLRNSGVTIILTTHYIEEAEEMADRVGVIAKGEIILVEDK
                     AEMMRKLGKKQLVLQL"
BASE COUNT          682 a          988 c          919 g          619 t
ORIGIN      
        1 gccccgtgat ttccgtcggc cagttcaggc cacgcacgac gtcgatcacc ctggtcttga
       61 tggtcgcgtc accgtcggcg cggatgatgg catcgtgaaa accaggtgga acaagcgcct
      121 cctcggtcat aagcaagcgc gaaccgagca gcacgccatc agcccccagc atgagcgcgg
      181 cggcaagacc gcgcccgtca cctacaccac cggccgcgac caacagcgta tccggcgccg
      241 ccttcaccag atagtcggcg acctcgggga caagcgtaaa cgtggtcctg gtctgcgcgt
      301 gcccaccagc ctcggtgccc tgcgcaacga caatatccgc tccggccgcg actgcctcac
      361 gcgcatgcgc catgctctgt atctggcaga caaggagcgc gcccgcggcc tttattggtt
      421 gagcaaacag cgccggcacg ccaaacgaaa gcatgattgc ggccggcgcg tgcgtcagca
      481 ctttgtcgag caattgcggt tgttgcgcca atgaccaggt gatgaaaccg catcccaccc
      541 gtgtatttcc cgccgaggca aattcccgtt caagccattc ggcgtcacca tagccgccgc
      601 cgatcagacc aagtccgccc gcagcggtga cagcagccgc gagtcggccg cccgccacga
      661 atcccatcgg cgccgaaatg acgggatgct cgatgccaag ccgttcggta aggcgcgttc
      721 gcaacgtcgt ctccgttatc cgtgcgcaaa gtcccaatac agttcgcgcg cctttcgata
      781 gagcggcccc ggctgcaacg agcgttcgtc gatgcggttg accggcatca ccttggagta
      841 attgcccgac gagaaaatct catccgcgtt ctgcacatcg cggtagctca atattgtttc
      901 gattacgtcc acgccggcct cgcgcagaag cttgatgacg cgctgacggg tgatgccatt
      961 aagaaatgtg ccgttcggcg ccggagtgaa tacgacgcct ccttttccga tgaaaatatt
     1021 ggcggtggcc aactccgcca cgtttcccag caggtcaagc accacacagt tgccgaaacc
     1081 gcgcgtgttc gcttcgagaa gcgcacgcgc attgttggga tagaggcagc cagccttggc
     1141 gttgaccggc atgcactcga gactcggctt gcggtaaggc gacagcgtca cggaaaagcc
     1201 ctcaggcttg cgcatcggag cttcatagag actcaggcac cattttgtgg actccggatc
     1261 gggcgcaacc agcaatgtgc cgctgcgctc ggcccaatac attgggcgaa tgtagagagc
     1321 cgcatccttg tcgaagcgtt tgacgccctc gcgggtcaga tcaagccatt gatcagcgga
     1381 gacaaccggc ttgaggtgca tcgtttcggc agaagcattg acccgcgcgc aatgcaaatc
     1441 gaggtccggc gacacgcctt cgaaggcgcg agcgccatcg aataccgaag aacaaagcca
     1501 ggccgcgtga tcgcgcacgc ccatgatcgc cacgttgcct tcgcgccatc cgccttcata
     1561 aaacgtccag gtcttcgacc agctttgtcc cggcatagcc gtctccatcg cgagccatac
     1621 gcgggcttga cctgcgcctc catgttgcgc ttccactgcg gaagggttca tggatcgccg
     1681 gatcccggca atgacgaact agaggcgcaa tcttacgata ccgccgccgc aaaagaccat
     1741 ggactcctca tttggaggaa ttgatgtcga tttttgtttt tgatgcctac gggacattgt
     1801 tcgacgtgca cgctgcgata gcgcggcatc gacaggagat gggacctgat gccgaccgga
     1861 tctcggccgt ttggcgaacc aagcagcttg aatacagctg gacgctcacg ctggccggac
     1921 attacacgga cttctggaca ttgaccgaac gtgcgctcga ttacgctctg gcgctcgtcc
     1981 ctgacgcaaa aaagacgctc aggccgaagc tgctggacgc ctacttcaag ctggacgcat
     2041 tccccgacgc gcgcagcact cttcaatcgt tgaaggcaaa aggccgcaag accggcattc
     2101 tctccaatgg atcaccggct atgctcaagg gcgcagtgga tgcggccggc atcggcagca
     2161 acctcgacgc gctgttgtcg gtggacgcgt tgaaaatgtt caaaccgcga ccggaggtct
     2221 atggtcttgt tacacgacac ttcggatgca agcccggcga tgtcattttc gtctcctcaa
     2281 accgctggga cgtgatggcg ggcgtatccg tcggtttccg cagcatgtgg atcaaccgcg
     2341 cgaaaatgcc cgacgagtac actgactttg cgcccgccca ggtccttgcc gatctgaacg
     2401 cattgacgga tttcgtttag gactttggtc gcatcgagtt cggctggcgg aaccgcgcat
     2461 cccggcctat atggcgcctg cacctttccg aacgattcat ctccatgcaa ccgatcatct
     2521 ccgtcaaagg cctgtcaaaa agctatgcgt ccggcttcaa ggcgctgagc ggtatcgacc
     2581 tcgacatccg ccgtggcgag atttttgcgc tgcttgggcc aaacggagcc ggcaaaacca
     2641 cattgattgg catcatctgc ggcatactgg tgccaacgca aggcaaggtg acggtcgatg
     2701 gccatgacat catcaaggac tatcgtgcgg cacgctcgct gatcggacta gttccgcagg
     2761 aattgcacac cgacgccttt gagagcgtct gggcgaccgt gtcgttcagc cgtggactgt
     2821 tcaacaagcc gaaaaatccg gattacgtcg agaaagtgct tcgcgcactc tcgctctggg
     2881 acaagaaaga caacaatatc attacgctgt caggcggcat gaagcggcgg gtgctgatcg
     2941 ccaaggctct gtcgcacgag ccgcaaatcc tgttccttga cgaaccgaca gcgggtgtcg
     3001 acgtcgaatt gcgcaaggac atgtggcaag tcgttcgtga ccttcgcaat tccggcgtga
     3061 cgatcatcct gaccacccat tacatcgaag aggccgagga aatggccgat cgcgtcggcg
     3121 tcattgccaa gggcgagatt atccttgtcg aggacaaggc cgagatgatg cggaagctcg
     3181 gaaagaagca gcttgtgctg cagcttca
//
LOCUS       DASSRL010000115         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_300667, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000115 DASSRL010000000
VERSION     DASSRL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300667"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..736)
                     /locus_tag="VFL49_02460"
                     /pseudo
     CDS             complement(143..736)
                     /locus_tag="VFL49_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
     gene            complement(748..1944)
                     /locus_tag="VFL49_02465"
     CDS             complement(748..1944)
                     /locus_tag="VFL49_02465"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HET7910868.1"
                     /translation="MASSPREVWITGVGILSCLGEGADAHWQKLMGALPSADITAFAP
                     FVVHPLAPINFDAQIPKKGDQRQMENWQRIGTYAAGLALDSAGVKNNPDILSRMDMIV
                     AAGGGERDLTVDSNILTGIRHAANPAAFLNERLMGDLRPTLFLAQLSNLLAGNISIVH
                     GVTGSSRTFMGEESAGIDAVRIAHARIGAGQSDIALVGGSYNGERPDLLMLYEFANLA
                     LKNKYVPVWERGNGNGGFALASLGAFLVVEAREHAEKRGVKALGRLTSVVSDRASRKP
                     GTITEALARLWDSLKSKVDPGYIAVLSGATGANPATAEERSFLEAHRDLAVRATGSYL
                     GHGLEPQFAMNIALATVALDRGSLFPPCDSTGLERPMSGALRQVVITGVGHWRGEGLA
                     LVERAG"
     gene            complement(1937..2410)
                     /locus_tag="VFL49_02470"
     CDS             complement(1937..2410)
                     /locus_tag="VFL49_02470"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HET7910869.1"
                     /translation="MRLEYFQLIDRIVGISLADRKISTEATVPATNTIFEGHFPGFPL
                     MPGVLLIEAMAQTSGWLVVAVNRFERMAFLAAVKDAKFRMFVTPGQKLSVKATLVHEG
                     SGYAITKAGIALGGKPICDAVLTLRVMDFPNHEMRLKMRETAARVGFPAEVPANG"
BASE COUNT          495 a          738 c          715 g          482 t
ORIGIN      
        1 gctcctattg gcgcgagcgc aaggcacgtg acaccatcgt cattgagcaa gcttcctcgg
       61 gattaatcgt tccatcaggg gataaaggag gcagctcaaa actccgcctt gtgtctgcac
      121 atagagtgac gacccactct cctatgcaaa gcgctgcatc tgtttcaccg cgacggatgg
      181 cttccacacc gagttgaatc gcggtcgccc cggatgcgca ggcggtcgaa agcgagatcg
      241 gtgagccttg agtgccgaat ttttcggcca ggttgtcggc gaccgatccg agcaggaagc
      301 ggcgatgata gtcgggaaat ttcgcgctca cgcgcagcat atcggcatag gtgacacttt
      361 cgttggcgcc gcaattcgcg ccgatttcga gccgctgcgg ccactccatt tcaatgggag
      421 gaacggcaag gaacagcggg ccgggaaaat tgccggccgt gccaagcgcg gattgtgcaa
      481 ccgcttcttc ggcggcgaga ttggcaaagg actcagtcaa cgccgcagcg gtgtcctgcg
      541 gcggcaggaa atcgatggtg ccggcaattc tggttttcag accgtcggtc ggaaagcgcg
      601 taatcgtctt aatgccggat tccccgccgg tgagttttgt ccagttatcg gctttgcccg
      661 ccccgagcga agtcaccacg ccaagtccag tgacggccac gacaggtcgt cccatcttgt
      721 ccttgcccgc cgccatagtt tctctcctca accggcccgt tcaacgaggg cgagaccttc
      781 gccgcgccaa tggcctacac cggttatgac cacctgcctc aacgccccgc tcatcgggcg
      841 ttccagaccg gtagagtcac agggtgggaa cagactacca cggtcgagcg caaccgtggc
      901 cagtgcgatg ttcatggcga attgtggctc cagcccatgc ccgaggtatg agcctgtggc
      961 gcgaaccgca agatcgcgat gcgcttccag gaagctgcgt tcttccgccg tcgcaggatt
     1021 ggcgcccgtc gcgccagaca gaacggcgat ataacccgga tcgaccttgc tctttagact
     1081 atcccaaagc cgcgctaacg cctctgttat cgtgcccggc ttccggctgg cgcggtcgga
     1141 gacgaccgag gtcagccgtc ccagtgcttt gacgccccgt ttttcggcgt gctcgcgcgc
     1201 ttcgaccaca aggaatgcgc caagcgacgc caatgcgaaa ccgccattgc cgttcccgcg
     1261 ctcccaaacg gggacatatt tgttcttaag cgcaaggttg gcgaattcgt acagcatcag
     1321 cagatccggg cgttccccgt tgtaggaccc accgaccagt gcaatatcgc tttgtccagc
     1381 gccaatccgt gcgtgcgcaa tccgcacggc atcaatcccc gctgactctt cgcccatgaa
     1441 cgtgcgtgaa gagccggtca caccatgaac gatcgagata tttccggcga ggagattgga
     1501 aagttgcgcc aggaacaagg ttggccgcag gtcgcccatc aggcgctcat tgagaaatgc
     1561 ggccgggttt gctgcgtgcc tgattcccgt aaggatgttt gagtcgacgg tcagatcgcg
     1621 ctcaccgcca ccggcagcaa caatcatgtc catgcgtgag aggatatccg gattgttttt
     1681 cacgccagcg gagtcgaggg caaggccggc cgcgtaggtg ccgatgcgct gccaattctc
     1741 catttggcgc tgatcgcctt tcttggggat ttgcgcgtca aaatttatcg gcgcaagcgg
     1801 atgtacgaca aaaggtgcga aggcggtaat gtcggcggaa ggcaacgctc ccatgagttt
     1861 ctgccagtga gcgtcagcac cttcaccgag acacgaaaga attccgaccc cggtgatcca
     1921 gacttctcgc ggactgctag ccattggccg gcacctcggc cgggaagccc actcgggcgg
     1981 cggtttcgcg catcttgagc cgcatttcgt ggttcggaaa atccataacg cgaagagtga
     2041 gcaccgcatc gcagattggc ttgccaccaa gcgcgatgcc ggctttcgtg atggcgtatc
     2101 ccgagccctc atgcacgagt gttgccttga ccgaaagctt ctggcccggg gtgacaaaca
     2161 tccgaaattt ggcgtctttt accgccgcaa gaaacgccat ccgctcgaag cgattcacgg
     2221 caacgaccag ccagcctgaa gtttgggcca tcgcttcaat cagcaacacg ccgggcatca
     2281 gaggaaaccc ggggaagtga ccctcgaaga tcgtgtttgt agcgggaacc gtcgcctcgg
     2341 tgctgatctt gcggtcggcg agactgatgc cgacgatccg gtcgatcagc tgaaagtact
     2401 caagacgcat gtgacccgcg cttcaggctc
//
LOCUS       DASSRL010000116         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_73269, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000116 DASSRL010000000
VERSION     DASSRL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73269"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFL49_02475"
     CDS             <1..1077
                     /locus_tag="VFL49_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HET7910870.1"
                     /translation="RTAAFVADRLREFGCDEVATGLGKTGVVGVIKGKKQGGNGDSRV
                     IGLRADMDALPIQEITNLPYASKTPGKMHACGHDGHTAMLLGAARYLAETRNFAGDAV
                     VIFQPAEEGGAGGAAMVKDGLMERFGIEQVYGMHNGPGIPIGSFAIRQGPIMAATDEV
                     DITIEGVGGHAARPHKAIDTVLVGCAVVNALQHIVSRNVDPLESAVISIPLFQAGHTD
                     NVIPQTAHLRGTARSFSPQVRDQLEKRITEVVEHTAKAHGAKAAVTYKRDYPVVVNTP
                     KEAEFASKVASEIVSESHVKPAIPVMGAEDFAFMLEKRPGAFIFMGNGNSANLHHPAY
                     DFNDKAIPFGTSYWVRLVENAMPA"
     gene            1179..1631
                     /locus_tag="VFL49_02480"
     CDS             1179..1631
                     /locus_tag="VFL49_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7910871.1"
                     /translation="MADAREYIFLMHELAHLMRTLFDQRARARGMTRAQWVILKRLDH
                     TPGLSQNELASLIEVEPITVGRLIDRLEARGLVERRSDPRDRRIHRLHLAAKAKPILR
                     EITQNIVEIDKIMSSGISEKALEELAAHLKTMKANLTLDESSLKKSAG"
     gene            1636..2775
                     /locus_tag="VFL49_02485"
     CDS             1636..2775
                     /locus_tag="VFL49_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET7910872.1"
                     /translation="MLFVPEEARQSSARKLILQRLNGAKNYVGRLKKDRKRLRLTLMF
                     GGAGFVAIAAAAFWLLGGRYVSTDNAYVHAAKLMVSTDISGIVSEVDVKEGQHVKKGD
                     VLFRVDPKQFQIALDSAKAQLELTRLNIESMKQDYQRILSDIASQQAQVELAKANYER
                     AEALVKSGSTSKANYDQMKYAYESGQKQLQALRDQAKMQLAKLNGNPDIDPKQHPQYL
                     RQLAQVEEMQRQLDHTVVRAPFSGIVTQVAALQPGTYLVAQTAALTNTGAVALVSDDN
                     LWIEANMKETDLTNVKVGDKVDVSIDTYPDREWTGTVESISPATGAEFSILPAQNTSG
                     NWVKVVQRITVRVKVEQRPQDPPLRAGMSAYVEIDTGRRHIADLL"
     gene            2785..>3378
                     /locus_tag="VFL49_02490"
     CDS             2785..>3378
                     /locus_tag="VFL49_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7910873.1"
                     /translation="MAAAAQTASKAESSHRYIITVCAMLATLMQALDNTIANVALPYM
                     QGSLSATSDQITWVLTSYVIAAAIMTAPVGWLTARFGRKNFFVVCLAGFTVASMLCGL
                     AESLTQIVAFRLLQGMFGAALVPLSQSTMLDIYTPEERGTAMAIWGMGVMVGPILGPT
                     LGGYLTDVYNWRWVFYVNVPFGILAIAGLWYFLQSNDK"
BASE COUNT          695 a         1036 c         1057 g          590 t
ORIGIN      
        1 cgcactgcag cattcgttgc cgaccggttg cgggagtttg gctgcgacga ggtcgcgacg
       61 gggcttggca agaccggtgt ggtcggggtc atcaagggca agaagcaggg cgggaacggc
      121 gacagcaggg tgatcgggct ccgtgccgac atggacgcgc tgccgatcca ggaaatcacc
      181 aatctgccct atgcctccaa gacgccgggc aagatgcacg cctgcggcca tgacggccac
      241 accgcgatgc tgctgggcgc ggcgcgctat ctcgccgaga cccgcaactt cgccggcgat
      301 gccgtcgtga tcttccagcc cgccgaggaa ggcggcgccg gaggtgcggc catggtcaag
      361 gacgggctga tggagcgctt cggcatcgag caggtctatg gcatgcacaa cggccccggc
      421 attccgatcg gctccttcgc aatccgtcag ggtccgatca tggcggcgac cgacgaggtc
      481 gacatcacca tcgaaggtgt cggcggccat gccgcgcgcc cgcacaaggc gatcgacacc
      541 gtactcgtcg gctgcgctgt cgtgaacgcg ctgcagcaca tcgtttcgcg caacgtcgac
      601 ccgttggaga gcgcggtcat ttccattccg ctgtttcagg cgggccatac cgacaacgtc
      661 attccgcaga ccgcgcatct ccgcggcacg gcgcggagct tcagcccgca ggtgcgcgat
      721 cagctcgaaa agcggatcac ggaagtcgtc gagcacaccg caaaggcgca cggcgcaaag
      781 gcggccgtca cctacaagcg cgattatcca gtcgtcgtga acacgccgaa agaggcggaa
      841 ttcgcgagca aggtcgcaag cgaaatcgta agcgaaagcc atgtgaagcc cgcgatcccg
      901 gtcatgggcg cggaggactt cgctttcatg ctggagaagc gcccgggcgc gttcatcttc
      961 atggggaacg gcaacagcgc gaaccttcat cacccggcct acgacttcaa cgacaaggcc
     1021 attccgttcg gcacgtcgta ttgggtgcgg ctcgtcgaaa acgcaatgcc ggcgtaatgc
     1081 aataaaatca gaggcttgtg ctgcgctatt cggccgcatg ggctttcctt gcgaaaatag
     1141 taacgtgcgt tatattatct ggcgttacgg tgattccgat ggccgacgcc cgcgaataca
     1201 tttttctcat gcacgagctg gcgcatctga tgcgcacgct gtttgaccag cgcgcccgcg
     1261 cgcgcggcat gacacgcgcg caatgggtga tcctgaagcg gctcgatcac acgccggggc
     1321 tttcgcaaaa cgagcttgcc tcgctcattg aagtcgagcc gatcacggtc gggcggctga
     1381 tcgaccggct ggaggcgagg ggcctcgttg agcggcgcag cgatccgcgc gaccgtcgca
     1441 ttcatcgcct gcacctcgcc gccaaggcga agccgatcct gcgcgaaatc acgcagaaca
     1501 tcgtcgagat cgacaagatc atgtcgagcg ggatcagcga aaaggcgctg gaagagctcg
     1561 cggcccatct taaaaccatg aaggcgaacc tcaccctcga tgagagctcg ctgaagaaat
     1621 ccgcagggtg aagtcatgct gttcgttccg gaagaagcgc ggcaatcgtc cgccaggaaa
     1681 ctcatacttc agcgtctgaa cggcgcgaag aattacgtcg gcaggctcaa gaaagatcgc
     1741 aagcgccttc gcctgacgct catgttcggc ggcgctggat ttgtcgcgat cgcggcggcg
     1801 gcgttctggc tgttgggcgg gcgctatgtc tcgaccgaca acgcctatgt gcacgccgcg
     1861 aagctgatgg tctcgaccga catttccggc atcgtgtcgg aagtcgacgt caaggaaggc
     1921 cagcacgtca aaaagggcga cgtgctcttt cgcgtcgatc cgaaacagtt tcagatcgcg
     1981 ctcgacagcg cgaaagcaca actcgagctg acgcgcctca acatcgaatc gatgaagcag
     2041 gattatcagc gtatcctgag cgatatcgcg agccagcagg cgcaagtgga acttgcgaag
     2101 gcgaattacg agcgcgccga ggcgctcgtg aaatccggca gcacgtcgaa ggcgaattac
     2161 gaccagatga aatacgccta cgagtccggc cagaaacagc ttcaggcgct gcgcgaccag
     2221 gccaagatgc agctcgccaa attgaacggc aaccccgaca tcgatcccaa gcagcatccg
     2281 caatatctgc ggcaactggc gcaggtcgaa gagatgcagc gccagctcga ccatacggtc
     2341 gtgcgggcgc cgttcagcgg gatcgtgacg caggtcgcgg cactccagcc cggcacttat
     2401 cttgtcgcgc aaaccgccgc gctcacgaac acaggcgccg tcgcgctcgt ttccgacgat
     2461 aatttgtgga tcgaagcgaa catgaaggaa accgatctca ccaacgtgaa ggtcggcgac
     2521 aaggttgacg tttccatcga cacctatcct gaccgcgaat ggaccggcac cgtggaaagc
     2581 atcagcccgg cgaccggcgc cgagttctcg atcctgcccg cgcagaatac gagcggaaac
     2641 tgggtgaaag tcgtccagcg catcaccgtg cgggtgaagg ttgagcaaag accgcaggat
     2701 ccgccgctgc gcgccggcat gagcgcttac gtcgagatcg ataccggccg ccggcatatc
     2761 gccgatctgc tctgagggcc ggtcatggca gccgccgcac aaaccgcttc gaaagcggag
     2821 tcttcgcatc gctacatcat cacggtgtgt gcgatgctgg cgaccttgat gcaggcgctc
     2881 gacaacacga tcgcgaacgt cgcgctgccc tatatgcagg ggagcctgtc ggcgacgtcc
     2941 gatcagatca cctgggtgct gacttcctac gtgatcgcgg ccgcgatcat gacggcgcct
     3001 gtcggctggc tcaccgcgcg cttcggccgc aagaatttct tcgttgtttg cctcgccggc
     3061 tttacggttg cctcgatgct gtgcgggctc gcggaatcgc tcacacagat tgtcgcgttt
     3121 cgcttgctgc aaggcatgtt cggcgcggca ctcgtgccgc tctcgcagtc gacaatgctc
     3181 gacatctaca cgccggagga gcgcggcacg gcgatggcga tctggggcat gggcgtgatg
     3241 gtgggcccga tcctcgggcc gacactcggc ggctatctca ccgatgtcta taactggcgc
     3301 tgggtatttt acgtcaacgt gccgttcggc atcctggcga tcgcggggct atggtacttt
     3361 ctgcaaagca acgacaag
//
LOCUS       DASSRL010000117         5068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1332884, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000117 DASSRL010000000
VERSION     DASSRL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332884"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1384)
                     /locus_tag="VFL49_02495"
     CDS             complement(137..1384)
                     /locus_tag="VFL49_02495"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HET7910874.1"
                     /translation="MDRTCVAVLATLDSKHEAARFICDALTAAGATPWLVDLSLRSHT
                     HDFADVNAGAIATSAGLAWVDLERLARADATKTMIAAGTEIVRDKFMRREINGVIGVG
                     GANGSTIACAIMRTLPLSFPKCMVTPVAATAAVQWYVAESDIAMYPTIGDISLNRVTR
                     AAMTNAAHAVAAMAKVWSRQAIAVRVPLLGISSFGNTQPAVDRITARLETEGFEVIHF
                     HASGPGGRALEKLAASGELAGVIDLTTSELTDLVTGGVYSAGEMRLTAAGAAGLPQVV
                     VPGCLDFTNWWVGQVPDKYRQREFYQYNVEIMLMRTNADEFSRLGQLFGERLRTAKGP
                     VSVLIPGGGMSQLVGRETYDIAGNERGAWAQPETDRVFCETLKRYLPTGIIKELPYHI
                     NDAGFADACAEEMIRMMRTPSKV"
     gene            complement(1385..2233)
                     /locus_tag="VFL49_02500"
     CDS             complement(1385..2233)
                     /locus_tag="VFL49_02500"
                     /inference="COORDINATES: protein motif:HMM:NF020927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HET7910875.1"
                     /translation="MAGKHNRAAILERLRAEIRSGRPVYDALCGSGITAKMAARGGAD
                     MVTTHCLAYFRMQGMSSMAGYLPICDANALTLELGERTLVNVVGDCPLIAGLLCVDPT
                     RDMARFLETIAAAGYDGVMNCPTVALIDGKFRSDLEETGMGFAREVDVLGCASRMGLF
                     TKAFCTSAEEALAMAEVGVDNIIVHFGNSSGGTIGSKTVLGDDASRARAGMVLDALSA
                     KFSDRIVTCHGGAIETPADFERFLKLEPRLHGYVGGSSAERFPIETSVVQATRQFKSV
                     KLSKAY"
     gene            2301..2738
                     /locus_tag="VFL49_02505"
     CDS             2301..2738
                     /locus_tag="VFL49_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7910876.1"
                     /translation="MQVFIENLAGSRRKNIYDERALRHLSTVDVSASYPYPYGFVIGT
                     VSGDGDAVDCFVLTSEPLAAGTILECDPIGLLEQIEDGEIDHKVIAVPSGASATIDDT
                     VIAALKKFISGVFSHIPDKRMEIGRLLDRDAAQNYLRRCRGSG"
     gene            complement(2774..3832)
                     /locus_tag="VFL49_02510"
     CDS             complement(2774..3832)
                     /locus_tag="VFL49_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7910877.1"
                     /translation="MRVAQVAPLTEAIPPKLYGGTERVVYWLTEELVALGHEVTLFAS
                     GDSCTSAKLETTWPRALRLDGPIRDPNALHMVMLERVRQRSDEFDLLHFHLDYYPFSL
                     FMRQKTPFITTLHGRLDLPEHQPVFSTFREVPLVSISNSQRRPVREANWIRTIYHGVP
                     EQLLTPRPIKPSYLAVLGRIAPEKSIDRAIRIANRCGIPLKIAAKVDRVDQDYYDEVI
                     SPLLKSPNIEYIGEIGDHEKSDFLSGAIALLVTIDWPEPFGLVMIEAMACGTPVIGFN
                     RGSVAEVVEDGLTGFIVEDEISAIHAVDRIRQLDRSKVRRRFEERFTARRMAKDYLAA
                     YQSIMELETPRLKVIAAE"
     gene            4094..>5068
                     /locus_tag="VFL49_02515"
     CDS             4094..>5068
                     /locus_tag="VFL49_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910878.1"
                     /translation="MKNLSCYSGRPFAFVGRYVRSRLLSHSVIVGAVMAAVLCSVGTQ
                     YGIKFLVDSLAANLSHDVWLAFAILGLLIASDNLLWRLASWIASGTFVQVTRDLRRDL
                     FRHLTGHAPSYFAGRLPGMLTSRITATSNAVYAMENMFVWNVLPPCLATVSAIVLVAT
                     ISLPMASILSVVGCFIVLAMFRLAAAGKPLHHDFAAKAATVDGEMVDVVSNMTLVKSF
                     CGILREHDRFDAALARETASRRRSLLFLEKLRLAHATITAAFTIGLVAWAILLWRRGA
                     ATPGDVVLICTLGLAVLHATRDLAVALVDVTQHVARLSESLATLLVQHE"
BASE COUNT         1001 a         1543 c         1463 g         1061 t
ORIGIN      
        1 acggccgacg catcgaattt aacgtcagcg cgcgcgacga agttgaagaa atcggcaacg
       61 gcacgcacga gcgcatggtc gtcgacatgg cgcgccttac gcagcggctc gaggcaaagg
      121 ctcggcctta gccaactcaa accttcgacg gcgtgcgcat cattcggatc atttcttccg
      181 cacaggcatc cgcgaagccg gcatcgttga tgtggtacgg caactccttg attatgccgg
      241 ttggcagata gcgcttcagt gtctcgcaga acacccggtc ggtttcgggc tgcgcccacg
      301 caccgcgctc gttgccggcg atgtcatagg tctcgcggcc gacaagctgg ctcatgccgc
      361 cgcccggaat aagcacactg accgggcctt tggcggtacg caggcgctcg ccgaagagct
      421 ggccgagcct cgagaactcg tcggcattcg tccgcatcag cataatctcg acattgtact
      481 gatagaattc gcgctgccga tatttgtcgg gcacctgccc cacccaccaa ttggtgaagt
      541 cgaggcagcc gggcaccacc acctgcggca agccggcagc gccggcggcc gtgagtcgca
      601 tctcgccggc actgtagacg ccaccggtga caagatcggt gagctccgag gtggtgagat
      661 cgatcacgcc ggcaagctcg cccgatgccg ccagtttctc gagcgcacgg ccgcccggac
      721 cggacgcatg aaaatgaata acttcaaatc cctccgtctc cagccgcgcg gtgatgcgat
      781 ccaccgccgg ctgggtattg ccaaaggagg agatacccag cagcggcaca cgcacagcaa
      841 tcgcctgccg gctccacacc ttcgccattg cagcgacggc atgcgcggcg tttgtcatgg
      901 ctgcgcgcgt cactcggttg agcgagatgt cgccaatggt cgggtacatg gcgatgtcgc
      961 tttcggcgac gtaccactgc acggcagcgg tcgcggcaac cggcgtcacc atgcattttg
     1021 gaaatgacag cggcagcgtg cgcatgatcg cgcaggcaat ggtcgagccg ttggctcccc
     1081 cgacgcctat gacgccatta atttcgcggc gcatgaattt gtcgcgcacg atttcggtgc
     1141 cggccgcaat catcgtcttg gtcgcatccg cgcgcgcaag acgttcgaga tcgacccacg
     1201 ccagtcccgc ggaggttgcg atggctcccg catttacgtc ggcgaaatca tgagtgtgcg
     1261 accgcagtga cagatcgacg agccacggcg tggcgccggc cgcggtcagt gcgtcacaaa
     1321 taaagcgcgc tgcctcatgc ttgctgtcga gcgtggcaag aacagcgacg caggtcctgt
     1381 ccacctagta cgccttcgaa agcttgacgc tcttgaactg ccgcgtcgct tgcaccacgg
     1441 acgtctcgat cgggaaccgc tcggccgacg agccgcccac atacccgtga aggcgcggtt
     1501 cgagcttaag aaaacgctcg aaatcggccg gcgtctcgat cgcgccgcca tgacaggtta
     1561 cgatgcggtc ggagaatttc gcggacagcg cgtcgagcac catgcccgcg cgcgctcgcg
     1621 aggcatcgtc gccgagaacg gttttcgagc cgatcgtgcc gcccgagctg ttgccgaaat
     1681 gtacaatgat gttatcgaca ccaacttccg ccatagcaag agcctcctcc gccgaagtgc
     1741 aaaacgcctt ggtaaagagg cccatgcgcg acgcgcatcc aaggacgtcg acctcgcgcg
     1801 cgaatcccat gccggtttct tccagatcgc tgcggaattt tccgtcgatg agggcaactg
     1861 tcggacagtt catcacgccg tcatatcccg ccgccgcaat tgtctcgagg aagcgcgcca
     1921 tgtcgcgtgt cggatcgaca cagagcaacc ctgcgatcaa cgggcagtcg ccaaccacgt
     1981 tcaccagggt gcgctcgcct aattcgagcg taagtgcgtt ggcatcgcag atcggcaggt
     2041 agcctgccat cgagctcatg ccctgcatgc gaaaataagc aagacagtgg gtcgtgacca
     2101 tgtcggcgcc cccgcgtgcg gccatcttgg cagtgattcc ggagccgcac agcgcgtcat
     2161 aaaccggccg tccgctgcgg atctccgcac ggagccgttc aaggatagcc gccctattat
     2221 gctttcctgc catgcaatcc cctgcctgcc gactggatca gcagcgagac gagactagta
     2281 tagaggcaag cgggaaatcc atgcaggtct tcatcgaaaa cctggccggt tcgcgacgca
     2341 agaatatcta tgatgagcgc gcgctacggc atctgagcac ggtcgacgtg tcggccagct
     2401 atccgtaccc ttatggcttc gtcatcggca ccgtcagcgg cgatggcgac gcggtcgact
     2461 gctttgtgct gacctcagag ccgctcgcgg cgggaacgat actggaatgc gatccgatcg
     2521 gacttttgga gcagatcgag gacggtgaga tcgaccataa ggtgatcgcc gtcccctccg
     2581 gtgcctcggc cacgatcgat gacactgtca ttgcggcttt gaagaagttt atttccggcg
     2641 tcttttctca catcccggac aaacgcatgg agatcggacg cttgctcgac cgcgatgccg
     2701 cccagaacta tctgcgccga tgccgtggat cgggctgatg tccggcgatg ccagcacaaa
     2761 taagcattca cggtcattcc gcggcgatga cttttagccg cggcgtctcg agctccatga
     2821 tgctttgata agcggctaga tagtcctttg ccatgcggcg cgctgtgaag cgctcctcga
     2881 agcgtctacg caccttgctc cgatccaatt gacggattcg atcaacagca tggatggcgg
     2941 aaatttcgtc ctctacgata aaaccggtga gtccgtcttc gacgacttcc gccaccgatc
     3001 ctcggttgaa cccgatcacg ggcgtaccgc aagccattgc ttcgatcatt accagcccga
     3061 acggctcggg ccagtcgatt gtcaccaaca atgcaattgc cccgctgaga aaatctgatt
     3121 tctcatggtc gccgatctcg ccgatgtatt cgatgtttgg agatttcagc agcggggaga
     3181 tgacttcgtc gtaataatcc tgatcaaccc gatcgacctt ggccgcgatc ttcaacggta
     3241 ttccgcaacg atttgcgatc cgaatggcgc gatcaatgga cttctcgggc gctatgcggc
     3301 caagcacagc gaggtagctg ggcttgatcg gtcgaggcgt cagcagttgt tcgggtacgc
     3361 cgtgatatat tgtacggatc caattcgcct cgcggacagg ccgacgttgt gaatttgaga
     3421 tcgaaacaag cggcacttcc cgaaaggtac tgaagaccgg ctggtgttcc ggcaagtcaa
     3481 gcctgccatg cagggtggta atgaatggcg ttttctgccg catgaacaac gaaaaagggt
     3541 aataatcaag gtgaaaatgc agcaggtcga attcgtcgct gcgttgccgc acccgctcca
     3601 gcatcaccat gtgcagggcg ttcgggtctc gaataggccc atcaaggcgc agcgccctgg
     3661 gccacgtcgt ttcgagtttc gctgacgtgc acgaatcgcc actcgcaaac agcgtcactt
     3721 cgtgtccgag cgcaaccagt tcctcggtta gccagtatac gacgcgttct gtaccaccat
     3781 aaagtttcgg gggtatcgct tcggtcaacg gcgcgacctg cgcaacacgc attctttctt
     3841 ctcctctctt ctgtctcttc aggcgccctc cccgaagcga tcaaacccca cgcgcggcat
     3901 ggaacgtttg ggtttgaaaa ttgttccgtt gtactggggg cgaaaccaac ctggacggtg
     3961 cactggtgcc agtgatgacg atttcggcaa gttggtctga agagttcaca atggaatact
     4021 ttggtcctct cacgaagtcg aagaggtggc cataccattg caaccgggat tcttgcagga
     4081 ttcgccgccg cgtatgaaaa atctgtcgtg ttattccggg cgcccgtttg cattcgtcgg
     4141 tcgctatgtg cgctcgcgat tgctctccca tagtgtaatc gtaggagcgg tcatggccgc
     4201 agtgctgtgc tcggtcggca cgcagtacgg cattaaattc ctggtcgaca gcctcgcggc
     4261 aaatttatcc cacgatgtct ggttagcctt tgcgattctc ggtctgctga tagcctcgga
     4321 taatcttctc tggcggctgg ccagctggat cgcgagcgga acattcgtac aggtcacgcg
     4381 tgacctccgc cgtgatctgt tccgccattt gacaggtcat gccccgagct acttcgccgg
     4441 gcgtcttcca ggaatgctga ccagtcggat aaccgcgacc tccaacgccg tgtacgcaat
     4501 ggagaacatg tttgtctgga atgtgcttcc accttgcctt gcaacagttt cggcaattgt
     4561 cttggtcgcc accatcagct tgccgatggc aagcattctt tcagtcgtcg gctgtttcat
     4621 tgtgctcgcc atgttccgtc tggcggcggc cggtaagccg cttcaccatg attttgcggc
     4681 caaagctgcg acagtagacg gagagatggt agacgtcgtg agcaatatga ccctggtaaa
     4741 gtcattttgc ggaatactgc gcgagcatga tcgcttcgac gccgcactgg cgcgcgagac
     4801 ggcctcccgt cgccgtagcc ttctgtttct cgaaaaactg cggctcgcac atgcgacgat
     4861 taccgccgca ttcacgatcg gactcgttgc ctgggccata ttgctttggc ggcgcggcgc
     4921 cgccacgccg ggcgatgtgg ttttgatctg cacattggga cttgccgtcc tgcacgccac
     4981 ccgcgatctg gctgttgcgc tggttgacgt aacccagcac gtcgcgcggc tttcggagtc
     5041 ccttgccacg cttctcgtcc agcatgag
//
LOCUS       DASSRL010000118         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1158075, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000118 DASSRL010000000
VERSION     DASSRL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158075"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFL49_02520"
     CDS             <1..467
                     /locus_tag="VFL49_02520"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET7910879.1"
                     /translation="QNIDGLHTLAGTSAERLVELHGTNSLVECQTCKWRGDPEPHFEF
                     FRRHRTTPRCQCGGYLKPATISFGQSLDPSELARAEQAALDADLVVALGSTLSVYPAA
                     AFPLLAGQRGVPYVIINRGPTEHDGEPSVSLRLEGEVSEIFPAAVESAVGSH"
     gene            580..1752
                     /locus_tag="VFL49_02525"
     CDS             580..1752
                     /locus_tag="VFL49_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HET7910880.1"
                     /translation="MRFWSWSGIAILALGLIASHASGAESEFRGAWVATVYNLDWPSK
                     PGLSPAAQKTELRALLDRAAELHLNAILLQVRPASDALYASRFEPWSEFLSGEAGVSP
                     GYDPLAFATAEAHLRGLELHAWVNPFRAAISAKQKLPANHVAKKHPEWVRRFGKQLWI
                     DPGNPEARQYVINVIIDMLGRYDVDGVHLDDYFYPYPLRPGEATFPDDATWARFGRRA
                     GMSRGDWRRANINDFVHSLYRAVKATKPRARLGISPFGIWRPGVPSTIRAGVDAYGQL
                     FADARKWLAEGWVDYLAPQLYWSIEPEEQSFPVLLDWWRAQSHGKPIWPGIATERIGS
                     KRPAREITRQIELTRRGTSSPGQIHWSMKALMRNQGGIDRLLLNGPYARTAEHPPR"
     gene            complement(1755..>2989)
                     /locus_tag="VFL49_02530"
     CDS             complement(1755..>2989)
                     /locus_tag="VFL49_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET7910881.1"
                     /translation="FNIVGYGCTTCIGNSGPLHPHIEKAIADYDLVAASVLSGNRNFE
                     ARVHQNIKANFLMSPPLVVAFALAGRVDLDLAKDPLAKDKEGRDVYLRELWPSLNEIR
                     QVMQSALKPEAFRRLYRDFADQNPKWNEIASSTGEVYQWDEESDYIHEPPFFADFAME
                     PGHIEDIRDARPLGIFGDSVTTDHISPAGAIKPTSPAGQYLISRGIKPPDFNSYGSRR
                     GNDLVMTRGTFANVRIKNLMVPGVEGGVTKYFANGSKEGEQMSIFDAAMRYAETRTPL
                     IILAGQEYGTGSSRDWAAKGTRLLGVKAVVAASFERIHRSNLVGMGVLPLQFTQGTSV
                     QSLGLSGSETYSITGLSDSLRAGQTLTLEIKRAGGEVRSIPVKLRIDTPIEIDYYRHG
                     GILQFVLRQLLASNAGNS"
BASE COUNT          596 a          886 c          899 g          608 t
ORIGIN      
        1 cccagaatat tgacggcttg cataccctcg ccggaacaag cgcggagcgg ctggtcgaac
       61 tccacggcac caattctctg gtggaatgcc agacctgcaa atggcgcggc gaccctgaac
      121 cgcatttcga attctttcgg cgccatcgga caacgccacg ctgccaatgc ggcgggtatc
      181 ttaagccggc tacgattagt ttcggccaaa gtctcgatcc gagcgaactg gcgcgggcgg
      241 agcaagcggc gctcgacgca gatctggtgg tggcactcgg ctcgactctg tcagtctatc
      301 cggcagccgc gtttccactc ctcgcgggac agcgcggcgt gccctatgtc atcatcaacc
      361 gcgggccaac ggaacacgac ggcgaaccga gcgtttcgct gcggctggaa ggagaggtga
      421 gcgaaatttt tcctgctgcg gtcgagagtg cggtcggctc acactagtgc ctgcgactgt
      481 cgaaaaatct caattgtgaa catcaaggac tacgatcatc acacaccggt gtcggcggcc
      541 agggcccgtc aaaaaaccca agttgcgcta acctgtgcga tgcgattttg gagctggtca
      601 ggcattgcta ttcttgcgtt aggcctgatc gcctctcacg cctcgggcgc ggaaagcgaa
      661 tttcgcggcg cctgggtggc gacggtttac aatcttgact ggccctcgaa accggggctt
      721 tcgcccgcgg cgcaaaaaac ggaattgcgc gccctgctcg atcgcgccgc ggaactgcat
      781 ctcaatgcca ttctcttgca ggtgcgcccg gcaagcgacg cgctttatgc ttcccgcttc
      841 gagccttggt cggaatttct cagcggagaa gcgggggtct caccgggata cgatccgctc
      901 gcatttgcca ccgcagaggc gcacctgcgg ggactcgaat tgcacgcctg ggtgaaccca
      961 tttcgcgccg cgatcagtgc caaacagaaa cttcccgcca atcatgtcgc gaaaaagcat
     1021 ccggaatggg tccgtcgctt cggcaaacaa ctctggattg atcccggaaa tcccgaagca
     1081 cgccagtatg tgatcaatgt gatcatcgac atgctgggac gctacgatgt cgatggagtc
     1141 catttggacg actatttcta tccctatccg ctgcggccgg gcgaggcgac ttttcctgac
     1201 gatgcgacct gggcccgatt tggacggcgg gccgggatga gtcgtggcga ttggcgtcgg
     1261 gcgaacatca acgacttcgt gcattcgctt taccgcgcgg tgaaagcgac caagccgcgt
     1321 gcgcggttgg gaatcagtcc ttttgggatc tggcggcccg gagtaccgtc caccattcgc
     1381 gcgggagtag atgcctacgg gcagctattt gccgacgccc gcaaatggct cgccgaagga
     1441 tgggtcgatt atcttgcccc gcagctttac tggagcatcg agccggaaga acaaagtttc
     1501 ccggtgctcc tggactggtg gcgagcgcaa agccatggca agccaatctg gcccggaatt
     1561 gcgaccgagc gaatcgggtc gaaacggccg gcgcgcgaaa tcacccgtca aatcgaattg
     1621 acgcgtcgcg gcacctcgtc gccagggcag atccactgga gcatgaaggc cttgatgcgg
     1681 aaccagggcg gaatcgaccg tctcctcctc aacggacctt acgcccgtac ggcggaacat
     1741 ccgcctcgat gacgctaaga gtttccggca ttgctcgcca gcagttgccg aagaacgaac
     1801 tggagaatgc ctccgtgacg gtaatagtcg atttcgatcg gcgtatcgat tcgcagcttg
     1861 accggaatgc tccgaacttc tccgccggcg cgcttgattt caagcgtcaa cgtctggccg
     1921 gcccgaaggg aatcggaaag tccggttatg ctgtaagtct cagatccgct caatcccaac
     1981 gactgcacgc tcgtcccctg cgtgaactgc aacggcagga cacccatgcc caccagatta
     2041 gaacgatgga tgcgctcaaa gctggcagcg accacagctt tcactcccaa caaccgggtg
     2101 cctttcgcgg cccaatcgcg gctcgaccct gtgccgtatt cctgacccgc caaaatgatg
     2161 agcggcgttc ttgtttcggc atacctcatc gcggcgtcga aaatcgacat ctgctcgccc
     2221 tccttactcc cgttggcgaa atactttgtg acccctcctt ccacccccgg caccatcagg
     2281 tttttgatgc ggacgttcgc gaaggtgccg cgggtcatga cgagatcatt cccgcgccgg
     2341 ctgccgtagc tgttgaaatc cggcggctta atcccgcgcg agatcaggta ctgtccggcc
     2401 ggtgaagtgg gcttgatcgc accggcgggg gagatgtgat cggtcgtgac cgagtcgccg
     2461 aaaatgccta gcggacgcgc gtcgcgaatg tcctcgatgt gacccggttc cattgcgaaa
     2521 tcggcgaaga acggcggctc gtgaatgtag tcgctctcct catcccattg atagacttca
     2581 ccagtgctgg aggcgatttc gttccatttg ggattttgat cggcgaaatc ccggtagagc
     2641 ctgcggaacg cctccggctt gagcgccgat tgcatgactt gcctgatttc gtttaacgag
     2701 ggccagagct ctctgaggta aacgtctcgg ccctccttgt ccttggcgag cgggtctttg
     2761 gcaaggtcga ggtcgacccg gccggcgagg gcgaaggcca cgacgagggg gggtgacatc
     2821 aggaagttag ccttgatatt ttgatggacg cgggcttcga agttgcggtt tccggaaagg
     2881 accgaagcgg cgaccaggtc atagtcggcg attgcctttt cgatgtgggg atggagcgga
     2941 cccgagttgc cgatgcacgt agtgcagccg tagccgacaa tgttgaatc
//
LOCUS       DASSRL010000119         6784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1595387, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000119 DASSRL010000000
VERSION     DASSRL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595387"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFL49_02535"
     CDS             complement(<1..973)
                     /locus_tag="VFL49_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7910882.1"
                     /translation="MSALDAVLDRIDRDLDQSLDRLFALLRIASVSTDPAFADQCRAA
                     AEHVAADLESIGFETSVRATEGHPVVVGKTNGTSGPRVLFYGHYDVQPVDPLNLWKTP
                     PFEPRIEALPDGRKIIVARGACDDKGQAMTFIEACRAFKAVTGRLPLPITTMIEGEEE
                     CGSRNLFKFVRDNAAEFKLDLALVCDTAMWDAATPAITTSLRGLVYEDVKITCADRDL
                     HSGLFGGAAQNPIRVLGRIIAALHDDSGRVTIPGFYDGVPELPADIKADLAGLGLTAE
                     SFLGQVGLKLPAGESDRMVIEQITTRPTAEVNGIIGGYTGEGAKTVIP"
     gene            1345..1704
                     /locus_tag="VFL49_02540"
     CDS             1345..1704
                     /locus_tag="VFL49_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006165185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HET7910883.1"
                     /translation="MDELVGRLVANVGVDRETAEKAVGIILDFLRKEGPPDKVQALID
                     RLPGAEALLAQHSEAGGAFPMSGIMGAGTKMMSAGLSMGQVQSVTRETISYAREKIGD
                     DAVGEIVGAIPGLSQFV"
     gene            1753..2160
                     /locus_tag="VFL49_02545"
     CDS             1753..2160
                     /locus_tag="VFL49_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HET7910884.1"
                     /translation="MSYPITDIDGIDGDVAVLLKSVGIRSTDRLLEAARTVKGRKTLA
                     AKTGFNEKQLLYWANVADRMRIKGVRKEYAELLQAAGVDTVKELKYRNPINLARAMAD
                     ANRKRKLVQLLPSEKMVKRWIENAKLLPLKITY"
     gene            2311..3078
                     /gene="folP"
                     /locus_tag="VFL49_02550"
     CDS             2311..3078
                     /gene="folP"
                     /locus_tag="VFL49_02550"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7910885.1"
                     /translation="MGILNVTPDSFSDGGRFFEPGTAIAHARDMVAQGADIVDVGAES
                     TRPYGDPVPVSADDEKARLATVLPAVVALGVPVSIDTIKASIAAWALDQGAAIVNDVW
                     GLQRDPDMAPLVAKRRAPVIVMHNRESADAAIDIVGDVIDFFSRSLEVAARAGIAREN
                     IVLDPGIGFGKTPEQSITCIARLAEFKRFGLPLLVGASRKRFISTVTPSAPDGRIGGS
                     ISAHLLAVEKGAAIIRAHDVAETVQALRVAAAIEAAR"
     gene            3075..3443
                     /gene="folB"
                     /locus_tag="VFL49_02555"
     CDS             3075..3443
                     /gene="folB"
                     /locus_tag="VFL49_02555"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET7910886.1"
                     /translation="MSDRIFVKGLALHAYHGVMPHEAKVGQTFIIDLDLEIDLSAAAR
                     SDKVVDTVSYDKVVDCVSKAFCAQRLRLIEAAAGRAADAVLATFPRVRSVRIAIHKPH
                     APIAATFSDVGVTLVRSRHA"
     gene            3436..3918
                     /gene="folK"
                     /locus_tag="VFL49_02560"
     CDS             3436..3918
                     /gene="folK"
                     /locus_tag="VFL49_02560"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7910887.1"
                     /translation="MPEALLALGGNVGNSRVILDRAIGLLCDGKEVRLMARSSDYRTP
                     PWGFKYQPHFVNLAIAVETTLGPRELLARAQEIELRLGRDRLQEKRNGPRTADIDILA
                     YDDLTMNEPNLTLPHPRLFERAFVLVPLAEIAGDRVISGRRVDEAARAVDAGQIHRLP
                     "
     gene            3961..5817
                     /locus_tag="VFL49_02565"
     CDS             3961..5817
                     /locus_tag="VFL49_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7910888.1"
                     /translation="MSDDFPLAAEFPPATQNDWRKLVEAALKGASFEKRLVSQTYDSL
                     RIEPLYPRAAPAPPVVGRPAVPWQIIQRVDHPHAKVANRQALDDLENGATGLSLVFAG
                     SVGAYGYGLDGTKETIAAALDGAHLDAGIALELDLSAQHKDAGQNLAAVLKGRDVAPD
                     KTQIRFGYDPLGAKASSGSSPLPWPQIAKLFADIVANLSGRGLTGPFAVADGRSVHAA
                     GGSEAQELAFVISNAVEYLRLFTQTGIPLEAARKLVWFRLAADADELLTIAKLRALRK
                     LWARIEEASGLTPSPTFVSAETAWRMMSTRDPYVNMLRTTIAVTAAGVGGADAIGVLP
                     FTIALGLPDAFARRVARNMQLILLEESNLYRVADPAAGAGGIETLTGDMAKAAWSLFQ
                     EIEAAGGAAVAIEQGLLQRKVAATRAERLKAIARRKDALTGVSDYPDLGEMPVKVLDI
                     PRVSAPPMPASMTFEALPPMRLAEPFEALRDASDRVLAKTGKRPKVFLANLGTLSDFT
                     ARATFARNFYEAGGIEAVTNDGFKSRAEMVAAFKASGARLACLCSSDDVYAAEAAEVG
                     KMLCSTGAIVHLAGRPGEKEQTWRQAGVKDFIYIGCDALATLRSAHDILGDK"
     gene            5814..>6784
                     /locus_tag="VFL49_02570"
     CDS             5814..>6784
                     /locus_tag="VFL49_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7910889.1"
                     /translation="MSQIPNFAKVAFTEAAVRQGKGTANAWATPEDIAVKPGYSEQDI
                     AGLDFLDTYPGKAPYVRGPYPAMYAAQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGL
                     SIAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFSGIPLDQMSVSMTMNGAV
                     LPVLALYIVAAEEQGVPHAKLSGTIQNDILKEFMVRNTYIYPPGPSLRIISDIFAYTS
                     AEMPKFNSISVSGYHMQEAGATQDLELAYTLADGVEYVRAGLKAGLSVDQFAPRISFF
                     WAIGMNYFMEIAKLRAARLLWAKLMKPFNPDDPRSLSLRTHCQTSGWS"
BASE COUNT         1323 a         2132 c         2041 g         1288 t
ORIGIN      
        1 ccggaatcac ggtcttggcg ccctcaccgg tgtagccgcc gatgatgccg ttgacctcgg
       61 cggtcgggcg cgtggtgatc tgttcgatca ccatgcggtc ggattcgccg gcgggtagct
      121 tcagccccac ctggccgagg aaagattcgg cggtcaggcc gaggccggcg agatcggcct
      181 tgatgtcggc cggcagctcc ggcacgccgt cgtagaatcc cggaatggtg acgcggccac
      241 tgtcatcgtg cagcgcggcg atgatgcgcc cgagcacgcg gatcgggttt tgcgccgcgc
      301 cgccgaacag gcccgagtgc aggtcgcggt cggcgcaggt aatttttacg tcctcgtaaa
      361 ccaggccgcg caacgaggtc gttatcgccg gggtggcggc gtcccacatt gcggtgtcgc
      421 agaccaaggc aaggtcaagc ttgaactctg cggcattgtc acgcacaaat ttgaacaagt
      481 tgcgcgaacc gcactcctcc tctccttcta tcattgtcgt tatcggcagc ggcaaccggc
      541 cggtgactgc cttgaaggcg cgacaggcct cgataaaggt cattgcttgt cctttatcgt
      601 cgcatgcccc gcgcgccaca atgattttac gcccgtccgg cagcgcttcg atacgaggct
      661 caaacggcgg cgtcttccac aaattcaagg gatcgaccgg ctgaacgtcg tagtgaccgt
      721 aaaacagtac tcgcggcccg gacgtcccat tcgttttccc cacgacgact ggatggcctt
      781 ctgtcgcccg cacgcttgtt tcaaagccga ttgattcaag atccgcggcc acgtgctccg
      841 cggccgcacg gcattgatcg gcgaatgccg ggtcagtcga cacggacgcg atgcgcagca
      901 acgcgaacaa acggtcgaga ctctggtcga ggtctcggtc gatgcgatcg agaaccgcgt
      961 cgagggcgct catggacaac tccgtaatgc aaacctgagt gagtaacaga tgaccattat
     1021 acccttattt tatcggggtt tttcggcaac acctggaacc aaaacgaagc agctccgtcg
     1081 tctacatcag attctattat cgcaccaacg gcaaaatgca ttcgtggact tcgaggagtt
     1141 ttgctgccac agggctacgt cctgaatcgt acacgatcca cattttcaca cgaactttca
     1201 ttcaacatat caattcaaac gcgttggttg attgttgcaa cgcggtctca cctcggacaa
     1261 atgcgactta accccgcttg tcacggccga aatcgatgtt caatatggac ttcaatccgc
     1321 cacaagtgcg gggggtgagg ggtcatggac gaactagtcg gccgtctcgt cgccaatgtc
     1381 ggcgtcgatc gcgagaccgc ggagaaggcg gtcggcatca tccttgattt cctgcgcaag
     1441 gaggggccgc ccgacaaagt acaggctctt atcgatcggc ttcccggggc ggaagcctta
     1501 ctagcacaac attcggaagc cggtggcgcg tttcccatga gcggcatcat gggcgctgga
     1561 acgaaaatga tgtcggcagg gctgagcatg ggtcaggtac agagcgtcac gcgtgaaacc
     1621 atctcctatg cccgggaaaa aatcggcgac gatgccgtgg gggaaatcgt cggagcgatc
     1681 ccgggcttaa gtcagtttgt ttgaaccatt tcaccgaacg ataaatagaa gaaaaggcgg
     1741 aggaggcacg gaatgtccta tcccatcacc gatatcgacg ggatcgatgg cgacgtcgcc
     1801 gtccttctca aatcggtggg gatcaggtcg accgaccgtc tgctcgaggc cgcccgtacg
     1861 gtgaaagggc gcaagacact cgcagcaaaa accggtttta acgaaaaaca gctcctgtac
     1921 tgggcgaacg ttgccgaccg catgcgcatc aaaggcgtgc gcaaggagta tgcggaactg
     1981 ctgcaggccg caggtgtcga taccgtcaag gagctcaagt atcgaaaccc gatcaacctc
     2041 gccagggcga tggccgacgc caaccgcaag cggaagctgg tccagctttt accgtcggaa
     2101 aagatggtca agcgctggat cgagaacgcc aagctgctgc cgttgaagat cacctactga
     2161 cgcatccagg tgtgcttgac agccccccgc cgaccgcgca aagcgaccgg catgggaatg
     2221 gcccgaaccc tggaaattgg cgccggatcg gcgcgaaacg ccactccaaa cgtgcttgcg
     2281 gggctgcttg cgcccggtcg cccgctcatc atgggcattc tcaatgtcac gccggactct
     2341 ttctcggatg gcggccggtt ctttgagcca ggcacggcga ttgcccacgc gcgcgatatg
     2401 gtcgcgcaag gggccgacat cgttgatgtt ggagcggaat ccacccgccc ctatggtgac
     2461 ccggttccgg tttccgccga tgatgaaaag gctcggcttg cgacggtatt gccggcagtg
     2521 gtcgcgcttg gtgtgccggt gtcgatcgat accatcaaag cctcgatcgc cgcctgggcg
     2581 ctcgatcagg gcgcggcgat cgtcaatgac gtatggggtc tgcagcgcga tcccgacatg
     2641 gcccctcttg tcgcgaaacg ccgcgcgcca gtcattgtga tgcacaatcg cgagtcggcg
     2701 gatgcggcca tcgacattgt tggcgacgtg atcgactttt tctcacgctc gctggaagtc
     2761 gccgcccgtg ccggaatcgc gcgcgagaat attgtgctgg atcccggcat cggtttcggc
     2821 aagacacccg agcagagcat cacctgcatc gcccggcttg ccgagttcaa acgctttggc
     2881 ttgcccctgc ttgtaggggc gtcgcggaag cgattcatca gtaccgtgac accctccgcg
     2941 ccggatgggc gcatcggtgg ctcgatctct gcgcacctgc ttgcggtcga aaagggagct
     3001 gcgatcatcc gggctcatga cgtcgcggaa accgtgcagg cgttgcgcgt agccgccgct
     3061 atcgaggccg cacgatgagc gaccgcattt tcgtcaaggg tctcgcgctg cacgcctatc
     3121 acggcgtgat gccgcacgaa gccaaggtcg gacaaacctt catcattgat ctcgacctcg
     3181 agatcgacct ttcggccgct gcgcgctccg acaaagtcgt cgacaccgtc tcctatgaca
     3241 aagtcgtcga ctgcgtgagc aaagcgtttt gtgcccagcg ccttcgcctg atcgaggctg
     3301 ccgcgggccg cgcggccgac gccgtgctcg cgacctttcc gcgcgtgcga tccgtgcgca
     3361 tcgcgataca caagccacac gccccgatcg ccgccacctt cagcgacgtc ggcgtaacgc
     3421 ttgtgaggag ccgccatgcc tgaagcgcta ctggcgctgg gcggcaatgt cggaaacagc
     3481 cgcgtcattc tcgatcgcgc aatcggcctg ctctgcgacg gaaaggaagt gcgtctgatg
     3541 gcccgctcgt ccgactaccg gacgccgccg tggggcttca aatatcagcc gcatttcgtc
     3601 aacctcgcga tcgcggttga aacgacgctt ggcccgcgtg agctgctggc gcgcgcgcag
     3661 gagatcgagc tcaggctcgg ccgcgaccgc ctgcaggaga agcgcaatgg tccgcgcact
     3721 gccgacatcg atatccttgc ctatgacgac ctcacgatga atgaaccaaa cttgacgctg
     3781 ccgcacccgc gcttgttcga gcgcgctttc gtccttgtac cgctggcgga aatcgctgga
     3841 gatcgtgtca tttcgggccg gcgtgtcgac gaggctgccc gtgccgtgga tgccggccag
     3901 atccatcgcc tgccgtgacg ttcgatagcc ccatatggcc gactgtgcca ttttgcccga
     3961 atgagtgacg atttccccct tgccgccgag ttcccgccgg ctacccaaaa cgactggcga
     4021 aagctggtcg aggctgccct taaaggcgcc tcgttcgaaa aaagactggt gagccagacc
     4081 tatgacagcc tgagaattga gcccctctat ccccgtgcgg cgcccgcacc gccagttgtc
     4141 ggccgccccg ccgtcccgtg gcagataatc cagcgggtcg atcatcccca tgcaaaggtg
     4201 gccaacaggc aggccctgga cgatctcgag aacggtgcca ccggattgag ccttgttttt
     4261 gccggcagcg tgggcgccta cggttatggc ctcgatggca caaaggaaac gatcgccgcc
     4321 gcactcgacg gcgcccacct ggacgccggc atcgccctcg aactcgatct cagcgctcag
     4381 cacaaagatg ccggacaaaa tctggcggca gtgctgaagg ggcgcgatgt cgcaccggac
     4441 aaaacacaaa ttcgcttcgg ctacgatccg ctcggtgcca aggcaagcag cggcagttcg
     4501 cctttgccct ggccgcagat cgcgaagcta ttcgccgaca tcgtcgccaa cttgtcggga
     4561 cgcgggctca cgggaccatt cgctgttgct gacggccgca gcgtccatgc agcgggcggc
     4621 agcgaagcgc aggagctcgc cttcgtcatc tccaacgcgg tcgagtatct gcgtctgttc
     4681 acacagacgg gtataccgct cgaagcggca cgcaagcttg tctggttcag gcttgccgcc
     4741 gacgccgatg agctcctgac catcgccaag ctccgggccc tgcgcaagct ctgggcgcgg
     4801 atcgaagaag caagcgggct gacgccaagc ccaacctttg tttccgcgga aacggcatgg
     4861 cggatgatga gcacgcgcga tccttacgtg aacatgctgc gcacgaccat tgccgtgacg
     4921 gcggccggcg tcggcggcgc cgatgcgatc ggcgtcctgc ccttcaccat cgcgctcggt
     4981 ttacctgacg ccttcgcccg ccgcgttgcg cgcaacatgc agctgatcct tctggaggag
     5041 tccaatctct atcgcgtagc cgatccggcc gcaggcgccg gcggcattga aacgttgacc
     5101 ggcgacatgg cgaaagcggc ctggagtctg tttcaggaga tcgaagctgc cggcggcgcg
     5161 gccgtcgcca ttgagcaagg cctcctgcag aggaaggtcg cggcaacgcg tgcggaacgg
     5221 ctcaaggcca tcgcgcgccg aaaggatgcg ctgaccggcg tgagtgatta ccccgatctg
     5281 ggcgagatgc cggtcaaggt gctcgacatt ccgcgcgtga gtgcgccgcc gatgcctgcc
     5341 agcatgacct ttgaagctct tccgccgatg cgtctggctg aaccgttcga agcgctacgt
     5401 gatgcttccg accgcgtgct tgccaaaacc ggaaagcggc caaaagtttt tcttgccaat
     5461 cttggcacgc tttccgattt caccgcgcgg gcgaccttcg ccaggaattt ctacgaggcc
     5521 ggcggcatcg aggccgtgac gaatgacggc ttcaaaagtc gggccgagat ggttgccgca
     5581 ttcaaggcat cgggcgcgag gctcgcctgc ctgtgttcct ccgacgacgt atatgccgcc
     5641 gaggcggcag aggtgggcaa aatgctctgc tcgaccggtg cgatcgttca tctcgccggc
     5701 cgaccgggtg agaaagagca aacctggcgg caggcgggcg tgaaagactt catttacatc
     5761 ggctgcgacg cacttgcgac gttacggtcc gcccatgata ttctgggcga caaatgagcc
     5821 agattccaaa cttcgctaaa gtggcgttca ccgaagccgc cgtccgccag ggcaaaggca
     5881 cggcaaacgc ctgggcaacc ccagaggata tcgcggtcaa acccggctat agcgaacaag
     5941 atatcgccgg cctcgacttc ctcgacacct acccgggcaa ggcgccctac gttcgcggcc
     6001 cctaccccgc gatgtatgcg gcgcaaccct ggacgatccg gcaatatgcc ggtttctcca
     6061 ccgctgagga ttccaatgcc ttttaccggc gcaatcttgc ggccggccag aagggtctct
     6121 cgatcgcttt cgatctcgcc acgcaccgcg gctacgacag cgatcacccg cgtgtctcag
     6181 gcgatgtcgg catggccgga gtcgcgatcg actcgatcta cgacatgcgc acgctgtttt
     6241 cgggaatccc gctcgatcag atgagcgtgt cgatgacgat gaacggtgcg gtgctgcccg
     6301 ttctcgcgct ctacatcgtc gccgcggaag agcaaggcgt tccgcatgcg aagctgtcgg
     6361 gcacgatcca gaacgacatt ctcaaagaat tcatggtgcg caacacctac atctatccgc
     6421 ccgggccaag tctgcgcatc atttccgata tcttcgccta tacatcggca gagatgccga
     6481 aattcaattc gatctcggtc tccggctatc acatgcagga agccggtgcg acccaggatc
     6541 tggagctcgc ttatacactc gccgacggcg tggaatacgt gcgcgcgggc ctgaaagccg
     6601 gactctccgt cgatcagttt gctccgcgta tctcgttctt ctgggcgatc ggcatgaatt
     6661 acttcatgga gatcgccaag ttgcgcgctg cgcgcctgct ttgggccaag ctgatgaagc
     6721 cgttcaaccc cgacgacccg cgctcgctct ccctccgcac gcactgccag acgtcgggct
     6781 ggtc
//
LOCUS       DASSRL010000120         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1228052, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000120 DASSRL010000000
VERSION     DASSRL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228052"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFL49_02575"
     CDS             complement(<1..562)
                     /locus_tag="VFL49_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7910890.1"
                     /translation="MNIMLEKVKLYTLLGDYPNTRALKTGEVTSDLIDFVFADVKVSN
                     TAFKPLVREARFDLGELAIVTYLQAKAYGKPYVLIPATVLGRGQHHTIAYNPGRGRLS
                     ASDLTGKRVGVRAYTQTTGAWVRGFLADDYNVDPARIRWVTFEDPHLAEYRDPGFVAR
                     APEGKAMIQMLLDGELDAAIVGDKLPD"
     gene            complement(567..1487)
                     /locus_tag="VFL49_02580"
     CDS             complement(567..1487)
                     /locus_tag="VFL49_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7910891.1"
                     /translation="MLYGGRSLLTFEAASPINASWTMPTTTIDIHPHIIANAAARYPL
                     SPLGGHQSEWSRARPVTLEQLIAAMDEAGVQKAAIVQASTCYGHDNSYVADAVGAYPN
                     RFTGVFSVDVLASDAPQHMRDWCTRGLTGMRLFTFGSTMSDQASWLDDPRTYPAWECA
                     SEMGLSVCLQMSAKAIPQATRMADRFPNVRIVLDHCARPDLADGPPYNAAASLFGLAS
                     HSNIYLKLTPRIFAESRRGKATPETFFPRLVSGFGASRLAWGSNYPSSEGALSQLLAT
                     AQESLSCLATGDRNWIFARTAQALYPALAD"
     gene            1680..>2500
                     /locus_tag="VFL49_02585"
     CDS             1680..>2500
                     /locus_tag="VFL49_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910892.1"
                     /translation="MIVSTTSTPVRADAYPSKPVRIIVPYGPGGIADVTMRLVADDLS
                     KRFGQQFFIENRPGAGGILGMQAAREAPADGYTLVMLGGGLTISKALFKSLPYDIETD
                     FIPISTTASYGLVITAKTGSSYKTIKDVIAAAKARPGALNFGTINAGSGQNLSAELFR
                     NLAGIDVTIIPYKTTPDLANAVLRGDVDVAFEYFAGFQSQITNGQMTVLATTGRERAS
                     NLPHVPIVGESGLTGYEVTSWNGLAVPKGTPSGIVTKLNDAVREALKSAEVQKFS"
BASE COUNT          520 a          746 c          739 g          495 t
ORIGIN      
        1 gatccggcaa tttatcgccg acaatggccg catcgagctc gccgtcgagc agcatctgaa
       61 tcatggcttt gccttcgggc gcccgcgcga cgaagccggg atccctgtat tcggcgagat
      121 gcggatcttc gaacgtgacc cagcggattc ttgccggatc gacgttgtaa tcgtcggcga
      181 ggaacccgcg cacccaggcg cccgtggtct gggtataggc gcgcaccccg acgcgcttgc
      241 cggtgagatc ggaagcgctc aaacgcccgc gcccgggatt gtaggcgatg gtgtggtgct
      301 gcccacgccc gagcactgtc gccggaatga gcacataggg cttgccgtaa gcttttgcct
      361 gcagataggt gacgatcgcc aattcaccga gatcgaacct tgcttcgcgg accaggggct
      421 taaatgccgt gttggaaact ttcacgtcgg cgaacacaaa atcgatgaga tcggacgtca
      481 cctcgcccgt cttgagcgct ctggtgttcg gataatctcc gagcagcgta tacaacttaa
      541 ctttctcgag cataatgttc acttattcag tccgcgagcg ccggatagag agcctgcgcc
      601 gtcctggcga agatccagtt ccgatcgccc gttgccaggc agctcagcga ttcctgtgcg
      661 gtcgccagga gctgagacag cgcgccctcg ctggaaggat agtttgaccc ccatgccaag
      721 cgagaagcgc cgaacccgga gaccagacgt ggaaaaaaag tctcaggcgt ggccttgcca
      781 cggcgcgact cggcgaagat ccgtggcgtg agtttgaggt agatattcga atgagatgcc
      841 aggccgaaca gactggcggc cgcattgtaa ggcggcccgt cggcgagatc gggacgcgcg
      901 cagtggtcaa gtacgatgcg cacgttcgga aaacgatcgg ccatgcgggt cgcctgcgga
      961 attgccttgg ccgacatctg caggcagaca gaaaggccca tctcgcttgc gcattcccag
     1021 gccggatatg tgcgcggatc atccagccag ctcgcctgat cggacattgt gctgccgaaa
     1081 gtgaaaagtc gcatgccggt gaggccgcgg gtacaccagt cacgcatatg ttggggcgca
     1141 tcggacgcca gcacgtcgac ggaaaacacg ccggtgaagc ggttcgggta cgcccccacc
     1201 gcatccgcca catatgaatt gtcatggccg taacaggtcg aggcttgcac gatcgcagct
     1261 ttctgcacgc cggcttcgtc catggctgcg atcaactgct ccagggtcac cggacgtgcg
     1321 cgcgaccatt cggattggtg tccgcctaat ggcgacagag gataacgggc ggcggcgttc
     1381 gcgatgatgt gcggatgaat atcgatcgtg gtcgtcggca tggtccagct ggcattgatc
     1441 ggcgacgccg cttcgaaagt cagaagcgac cgccctccgt agagcatggg acagcgaccg
     1501 taaagggcta ttggcatttc gattcgcggg aggcgtattg ttagtgacgt ggcctcctcc
     1561 tattgtccgc gtctatcgca actcaccgga agcggaacaa catgccctca ttcatccgtg
     1621 ccgcagctca tcaccttatg cgatgcgcca catcgattat ctccgctgcg tcactggcgt
     1681 tgattgtttc gacgacgtct acgccggtca gggccgatgc ttacccgtca aaacccgtgc
     1741 gcatcattgt gccttatggc ccgggtggaa ttgccgacgt caccatgcgc cttgtggcgg
     1801 acgacctcag caagcgtttc ggtcagcaat tctttataga aaaccggcca ggcgccggtg
     1861 gtattctcgg catgcaggcg gcccgggaag ctccagccga cggttacacg ctcgtcatgc
     1921 ttggcggcgg cctcacgatc tccaaagcac ttttcaagtc cctgccctac gacatcgaga
     1981 ccgacttcat tccgatttcg acgacggcat cctacgggct cgtaatcaca gccaaaaccg
     2041 gctcatcgta caaaacgatc aaggatgtga ttgccgcggc caaggcccgt cccggtgcgc
     2101 tcaacttcgg cacaatcaat gccggtagcg gccaaaacct ttccgcggaa ttgtttcgaa
     2161 atttggccgg gattgacgtg accatcatcc cctacaaaac gacgccggat ttggccaacg
     2221 ctgttctacg tggcgacgtt gacgttgctt ttgagtattt tgccggattc caatcgcaga
     2281 tcacaaacgg acagatgacc gtactcgcca cgaccggacg cgagcgcgcg agcaacctgc
     2341 cccacgttcc gatcgtcggc gaaagcggcc tgaccggtta tgaagtgaca agctggaacg
     2401 gccttgccgt tccaaaggga acgccctctg ggattgtcac caagctcaat gatgccgtgc
     2461 gggaagcact gaaatctgcc gaggtacaga agttctcaca
//
LOCUS       DASSRL010000121         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_965545, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000121 DASSRL010000000
VERSION     DASSRL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965545"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFL49_02590"
     CDS             <1..576
                     /locus_tag="VFL49_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET7910893.1"
                     /translation="VLMDLLDAVIDRSADILERIGAEVDQVSHSIFEPEEEVKPPSYN
                     DVLKALGRKGDLTSKVRESLVSIGRLLLFLANEADNMKWPKDMRAQLQSMQRDISSLS
                     DHATYLTNKITFLLDAMLGVVNIQQNNIIKIFSVAAVVLMPPTLVASIYGMNFRHMPE
                     LEWVTGYPFALFLMLLAAALPYLYFKWRKWL"
     gene            complement(618..974)
                     /locus_tag="VFL49_02595"
     CDS             complement(618..974)
                     /locus_tag="VFL49_02595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET7910894.1"
                     /translation="MPSSDRESFAPFDIVVVPFPYADRLAEKRRPALVISGNKLVPTG
                     LLWVAMITSIGNEPWPFDIPIPDLRRAGLPAPSIVRITKIACIEQARVDRRAGRLDKA
                     TARTVVQRLRGLLGVA"
     gene            complement(961..1158)
                     /locus_tag="VFL49_02600"
     CDS             complement(961..1158)
                     /locus_tag="VFL49_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7910895.1"
                     /translation="MSVKSQTVLPREVRARLGIRPGDTLRYRLTDQGVLIDKAPAEAD
                     DPFASFSEWSSEADQKAYAKL"
     gene            complement(1283..>2414)
                     /locus_tag="VFL49_02605"
     CDS             complement(1283..>2414)
                     /locus_tag="VFL49_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET7910896.1"
                     /translation="YRAINNYMARMVTTFGGLDSCIWVHDYHFLPLGAELRKHGAMCE
                     IGFFLHTPWPSPRAFSALPQSRELVEAMLAYDLIGFQTDTDRANFTELVRQELRIPCS
                     GSVIRSRFGTCRVATFPIGIDVQEFASRAQQSADDPDVQRLRTSLCGSKLLLGVDRID
                     YSKGLHQRIRALDSLLTQYPRLRRRVSMLQVAVPSRTSIETYRLLQREIAALTGEVNA
                     RHGEVDWAPIRYVTKSFCQTTLAGFYRASAVGVVTPLRDGMNLVAKEYVAAQDPANPG
                     VLVLSRSAGAARELDAALQVDPEDVEAIAFQIAAALSMPLAERQARWHQMMDVLLRGS
                     IHAWFAGFMQALKSTRPDAAGPVRVKLPAVQGSEQGLTAAHR"
BASE COUNT          485 a          719 c          741 g          469 t
ORIGIN      
        1 gtactgatgg atctgctcga tgccgtcatc gaccgctccg ccgacattct cgagcgtatt
       61 ggcgccgagg ttgaccaggt ctcgcattcg atttttgagc cggaggagga ggtcaaaccg
      121 ccgtcgtaca acgacgtgct caaggcgctc gggcggaagg gcgacctgac ttcgaaagtg
      181 cgggagagcc tggtgtcgat cggccgtctg ctgttgtttc ttgccaacga agccgacaac
      241 atgaagtggc cgaaggacat gcgcgcgcaa ctgcagtcga tgcagcgcga tatcagctcg
      301 ctgtcggatc acgccaccta tctcaccaac aagatcacgt tcctgctcga tgccatgctg
      361 ggcgtggtca atatccagca gaacaacatc atcaagatct tctcggtggc cgccgtggtc
      421 ctgatgccgc cgacgctggt cgcctcgatc tacggcatga atttcaggca catgccggag
      481 ctcgagtggg ttaccggcta tcccttcgcg ctgttcctga tgctgctcgc ggccgcgctg
      541 ccatatcttt atttcaaatg gcggaagtgg ctgtaagagc gagggcagaa gaagaaagcc
      601 tcgtctcgat gctcgtttta ggcaacgcca aggagccccc gcagcctctg tacgacggtt
      661 cttgcggttg ccttgtcaag ccgcccggca cggcggtcga cgcgagcctg ctcgatacag
      721 gcaattttgg ttatgcgtac gatcgatggc gcgggaaggc cggcacgccg aagatcggga
      781 ataggaatgt caaagggcca aggctcattc ccgatgcttg taatcatagc cacccaaagc
      841 agcccggtgg gaacgagctt gtttccggaa atgaccagcg ccggacggcg tttttccgct
      901 agacggtcgg cgtacggaaa agggacgacc acgatatcaa aaggcgcgaa actttcgcgg
      961 tcagagcttg gcataggcct tttggtcggc ttcgctcgac cactcactga acgatgcgaa
     1021 gggatcgtcg gcttcggccg gtgccttgtc gatcaaaacg ccttggtcgg tcagccgata
     1081 gcgcagcgtg tcgcccggcc tgatgccgag tctcgcgcgg acctcgcgcg gcaacacagt
     1141 ctggctcttg accgacacct tcgagaatgt gggcttttca gcgcgccgct tcatcttgcg
     1201 gggtactata acttaccgtc ttacgtctta caagatcgta agcgttggaa ccatggcatc
     1261 ggcgtgaacg aaaagttgat ctctaacggt gtgccgcagt cagtccttgc tcgctccctt
     1321 gcacggccgg cagcttgacg cgaacgggac ctgcggcgtc gggtcgcgtc gacttgagcg
     1381 cctgcatgaa accggcgaac caggcatgga tcgacccgcg cagcagaacg tccatcattt
     1441 gatgccagcg cgcctgacgc tccgcgagag gcatcgacaa tgctgccgcg atctggaacg
     1501 cgattgcctc gacgtcttcc ggatcgacct gcagcgccgc atcgagctca cgggcggcgc
     1561 ccgccgaccg cgacaggacg aggacgcccg gattggccgg atcctgcgcg gccacatatt
     1621 ctttggcgac aagattcatg ccgtcgcgca aaggggtgac aacgcccacg gcggacgcgc
     1681 gataaaagcc ggcaagcgtg gtctgacaga aactcttggt cacatagcga atgggcgccc
     1741 agtcgacctc gccgtgcctg gcatttacct ctcctgtgag ggccgcgatc tcgcgttgca
     1801 gtagccgata ggtctcaatg ctcgtgcgcg aaggcaccgc cacctgcagc atcgatacgc
     1861 gccgccgcag cctgggatac tgcgtcagca ggctgtctag ggcgcggatg cgctggtgca
     1921 ggcctttcga gtagtcaatc cggtcgacac cgaggagaag cttggagccg cacaggctcg
     1981 tgcgcaaccg ctgaacatcc ggatcatctg ccgattgttg cgcgcggctg gcaaactcct
     2041 gcacgtcgat cccgatggga aaagttgcga cccggcacgt gccgaagcgg ctgcggataa
     2101 ccgatccgga gcagggaatt cgcagctcct gccgtacaag ttcggtgaaa ttcgcgcggt
     2161 cggtgtcggt ctggaagccg atcaggtcgt aggccagcat ggcctcgacc aattcgcggg
     2221 attgcggcaa tgcgctgaaa gcgcgtgggc ttggccacgg cgtatgcaga aaaaatccga
     2281 tctcgcacat cgctccgtgc ttgcgcagct cggcaccaag cggcaggaaa tggtaatcgt
     2341 gcacccagat gcatgagtcc aatccaccga acgtcgtgac catgcgcgcc atataattgt
     2401 taatggcgcg gtat
//
LOCUS       DASSRL010000122         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1665505, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000122 DASSRL010000000
VERSION     DASSRL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665505"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFL49_02610"
     CDS             complement(<1..730)
                     /locus_tag="VFL49_02610"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET7910897.1"
                     /translation="MTAEPGTRVLAADDKAIEAAARCLAAGGLVAFPTETVYGLGADA
                     GNGEAVARLYAAKGRPAFNPLIAHVAGIDSARAAGVFTDAAEKLAAAFWPGPLTLVLT
                     KRPDCHIADLALAGLDTVAVRVPAHPVARALLAAFGGPIVAPSANRSGHVSPTSAAHV
                     LVDLRGRIDLIVDAGPCKVGVESTIISCAGEPGLLRPGGLPREGIERILGGPLTAASI
                     AEEAPPAPGMLASHYAPKTLLRLEA"
     gene            786..862
                     /locus_tag="VFL49_02615"
     tRNA            786..862
                     /locus_tag="VFL49_02615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:820..822,aa:Pro,seq:cgg)
     gene            1245..1877
                     /locus_tag="VFL49_02620"
     CDS             1245..1877
                     /locus_tag="VFL49_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910898.1"
                     /translation="MSITQTTAAAATETSGIFAEAVGGLATIALSIIALAGTAPDFLL
                     AIATMVFGAALLIEGTSIVTEYVLAAVPGAFGLAASGLAAVFLAGLTGIILGVLSLLG
                     LHAGVLTSAAVITFGSALLLNSSAILNLYSIKAPAGSEFIMGGAVGTAVAQALAGISA
                     LVLGILAITGNQPMSLNLVALLVMGAAILVTGNGMNNAVINMVRRIPERP"
BASE COUNT          397 a          656 c          679 g          413 t
ORIGIN      
        1 cggcctcgag acgcaataac gtctttggcg cgtagtgcga cgccagcatg ccgggcgccg
       61 gcggcgcctc ctcggcgatc gatgccgccg tcaacggacc accgagaatc cgttcaatgc
      121 cctcgcgcgg gagccctccg ggccgcagca agcccggctc accggcgcag gatatgatag
      181 tcgactccac cccgaccttg cagggtcctg catcgacgat gagatcgatc cggccgcgca
      241 aatcgacgag cacatgcgcg gcgcttgtcg gcgacacgtg accggagcga tttgcggaag
      301 gtgcgacgat cgggccgccg aaggcggcaa gcaatgcgcg cgccacggga tgcgcgggca
      361 cgcgcaccgc gacggtgtcg agtccggcca gtgcgagatc ggcgatgtgg caatctggcc
      421 gctttgtgag caccagcgtc agcggccccg gccagaacgc agctgccagc ttttctgcgg
      481 cgtcggtgaa tacgccggcg gcgcgcgcgg agtcgatgcc ggcgacatgc gcgatcagcg
      541 gattgaaagc cggccgtccc ttggccgcat agagccgcgc cacggcctcg ccgttgccgg
      601 catcggcacc aagcccgtag acggtttcgg tcgggaacgc gaccagcccg ccggcggcaa
      661 ggcagcgcgc cgcggcctca atcgccttgt cgtcggccgc taaaacgcgt gttccgggct
      721 cggcagtcat tacctttctc aagcttgcct ttagggacgc cggggttata agaccggcca
      781 tcagtcggag tgtagcgcag tctggtagcg caccacgttc gggacgtggg ggtcgcaggt
      841 tcaaatcctg ccactccgac catcacttag cagaaaactg ggcgcgacgg gccacagctt
      901 cacatgcatt gaaaacgcgt tcggcactcg agaggctatc tgggtggggg tactcaccgc
      961 ttcgcgagca tctcgatggc catgcgcgga cattcatttc gcggagccag ttctgcgcac
     1021 tcgctcctta ccgcatgatt ttcagttgta ctgaaatcaa aataagtatt taggtgcagc
     1081 tcgtggaacc ggcgcggggt ccgcgtgttc tagtctgtga aagggatttc gaacaatcaa
     1141 ttgtcgcgga attcccaggg ggcacaaatt ggaaccggcc gccatgtcgg ccccttactt
     1201 ttagatgacc tgacggcgcg gtcctccaac aataggaggg tcctatgagt atcactcaaa
     1261 caaccgctgc tgccgcgacg gaaacgagcg gcatttttgc ggaagcagtt ggtggcctcg
     1321 caaccattgc cctgtccatc attgcactgg ccggaacggc gcccgatttt ttgctggcga
     1381 tcgccacgat ggtctttggc gcggcgttgc tgatcgaggg cacatcgatt gtcaccgaat
     1441 acgtgctggc tgcggttccg ggtgccttcg gcctcgcggc cagcgggctc gcggctgtct
     1501 ttctcgctgg tctgaccggg attattcttg gcgtgctatc gcttcttggc ctccatgccg
     1561 gcgtgctcac ctctgccgcg gtcattacgt ttggcagcgc gctgctgtta aacagtagtg
     1621 cgatcctgaa cttgtacagc atcaaagctc ccgcaggctc cgagttcatc atgggcggag
     1681 cagtcggaac ggctgtcgcg caagcattgg ccggtatatc cgcgttggtt ctcggcatcc
     1741 tcgccattac cggcaatcag ccgatgtcgc tcaatctggt agccctgctt gtgatgggcg
     1801 cagcgatcct ggtgaccggg aacggcatga acaacgccgt tatcaatatg gtcaggcgga
     1861 ttcccgaacg gccatagaac gctcaccggg cgaattgtac tgagggcgaa cgcgcgtcag
     1921 agaccggctt tttggtcaaa gcgttcctgc gtggccgctt ggtcagcgcg gcgccaccaa
     1981 cgcgctcgac acgtcaaatc ccgaaaaatc gggcagaccc gggagggtct gcccggatcg
     2041 ctgagtgctg cgaagggctc gcaaaaaccg cagtgcggcc ggcaaggcta tgagcggcgc
     2101 acagcgtttg cagcgttgct gaaacgttag gcaaaatgta gctta
//
LOCUS       DASSRL010000123         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1175747, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000123 DASSRL010000000
VERSION     DASSRL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175747"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VFL49_02625"
     CDS             complement(<1..1631)
                     /locus_tag="VFL49_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910899.1"
                     /translation="MKKHRRRHAAAAVLAAISLFLQPLTPLLVAQTPAAQTAKPTAPS
                     AQQPAAKPATPAAAGSKPAVPAAAAAPIDGGWPRFYDAAGGSILLYQPQISNWDNQKH
                     LVAFSAVSFRKAAAGKPATGQAATGQTAPEKPAVGTIKLEADTKVSVADRLVSLSTIK
                     VTEANFQTLSKDQVQAITTAIDKAIPDDERVIALDRVLANIDKSQIVPKNVEGIKADP
                     PTIFFSKAPAVIMNIDGDPIWSPIKDNDLKYAINTNWDLFQYGPTKTLYLRNDASWLK
                     ATDVNGPWSPAGKLPDSFTKLPVEENWKDVKASLPGNAVAASAVPKIFISTKPAELIL
                     LTGEPAYQPVKGTGLMWVSNTESDVFRMGKAGEVYYLVAGRWFSAPDFVGPWKFATTS
                     LPEDFKKISLEHERSRVLASVPGTDQAAEAVLVAQIPQTARVNKKEVKAPEVAYGGDP
                     QFQPIEQTTVQRAVNTDKDVFKVGDSYYMCYNGVWFVGKTPTGVWEVASSVPQEIYKI
                     PVSSPANHVTYVTVEDDDSSDEWVTFAAAAGYTGM"
     gene            complement(1669..2028)
                     /locus_tag="VFL49_02630"
     CDS             complement(1669..2028)
                     /locus_tag="VFL49_02630"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HET7910900.1"
                     /translation="MPIDMKPEGKNVYLLEMWGQVKKAEFQKCEQRLAGEIGRVGPVK
                     LLFNLSGFEGWETRSDWNDLKFFIQHGDSIEQIAIVGDDKWKNQAMMFAGAGLRKAPV
                     EYFPEADLTKARAWLSS"
     gene            2403..2960
                     /locus_tag="VFL49_02635"
     CDS             2403..2960
                     /locus_tag="VFL49_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HET7910901.1"
                     /translation="MKKQFVVSMVALVLVVPALLLAQKTSYDFDKGANFAAYKTYAHK
                     DGTKVGQPLIDDRITSAIDSAMASKGFTKSDTPDVFVVYHVAFDKQKDISTFSSGYGG
                     GYGPYGWGYGGGWAGGTTTTQVRDILIGTLVIDLADAAKKQMVWRGMATKEGINPQQK
                     PDKLDKNINNAVAKIFKNYPPKQKG"
     gene            3073..3573
                     /locus_tag="VFL49_02640"
     CDS             3073..3573
                     /locus_tag="VFL49_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910902.1"
                     /translation="MVKRIFATITLLMLCGLVVHVQSPAASDKETRTTNLTAYTELLR
                     SDVRAEKVAILTEVMGFTEAEDKAFWPIYREYDAEMSKLGDERVALIGDFSSHYEQMT
                     DAAADQIATKALDLEARRQAVKAKYYDRVKKALSPRTALRYLQVEHQLQLIIDLQISA
                     ALPIAK"
     gene            3585..>4302
                     /locus_tag="VFL49_02645"
     CDS             3585..>4302
                     /locus_tag="VFL49_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910903.1"
                     /translation="MKYRPTHWITAVLVLGIGVSLSADRVKLKSGKVVEGMFVGADSK
                     AVRILLDTGKVTEIGIDDAVSVEFTPRKPTPPPPPPPNPATQPKPVQVPSGTPINVRL
                     TQGIDVDASKAGQSFKAIVDDPVMMNGSIVIPRGATAELQAVAVQQSGKMKGSDKISL
                     KMNSIGFGGNVYQVTTAYVETKGGGEGKKTARKVGGGAGLGAIVGGIAGGGSGAAIGA
                     AVGGATGAVVASGGEEHLKLP"
BASE COUNT          804 a         1306 c         1353 g          839 t
ORIGIN      
        1 atcatcccgg tgtagcccgc agcggccgcg aacgtcaccc actcgtcgct gctgtcgtca
       61 tcctcgaccg tgacgtaggt cacgtggttg gctggggagc tgaccgggat cttgtagatc
      121 tcctgcggca cggagctcgc gacttcccac acgccggtcg gcgtcttgcc gacgaaccag
      181 acgccgttgt agcacatgta gtacgagtcg ccgaccttga acacgtcctt gtcggtgttg
      241 accgcgcgct gcaccgtcgt ctgctcgatc ggttggaact gagggtctcc accatacgcg
      301 acttcaggag ccttgacctc cttcttgttg acgcgtgcgg tctgcgggat ctgagcgacc
      361 aggacggcct ccgcggcctg atcggtgccg gggaccgacg ccagcacgcg cgaccgctca
      421 tgctccagcg aaatcttctt gaagtcctca ggcagcgatg tcgtcgcaaa cttccacggg
      481 ccgacaaagt ccggcgcgga gaaccatcgt cccgcaacca ggtagtagac ctctccggcc
      541 ttgcccattc ggaacacgtc gctttccgtg ttgctcaccc acatcagacc cgtgcccttg
      601 accggctggt aggcgggctc gccggtcagc agaatcagtt ccgcgggttt ggtgctgata
      661 aagatcttcg gcactgccga tgcggcgacg gcgttgccgg ggaggctcgc cttgacgtct
      721 ttccagttct cttcgaccgg cagcttggtg aagctgtcgg gcagcttccc ggcgggcgac
      781 cacggaccgt tcacatcagt cgctttcaac catgaggcgt cgttccgcag atacagcgtc
      841 ttggttggcc cgtactggaa cagatcccag ttggtgttga tggcgtactt cagatcgttg
      901 tccttgatgg gactccagat cggatcgccg tcgatgttca tgatgaccgc gggagccttg
      961 ctgaagaaga ttgtcggcgg atcggccttg atgccttcca cattcttggg aacgatctgg
     1021 ctcttgtcga tgttggcgag cactcgatcc agggcgatca ctctctcgtc gtcggggatc
     1081 gccttgtcta tagcggtggt gatcgcctgc acctggtctt ttgagagtgt ctggaaattg
     1141 gcttcagtga ccttgatggt gctgaggctc acgaggcgat cggcgacgga cacttttgtg
     1201 tccgcttcga gtttgatagt acctaccgcc ggcttttcag gggcggtttg cccggtggct
     1261 gcctgtccag tggcgggctt gcctgcagcc gcctttcgga acgacacggc gctgaacgcc
     1321 accagatgct tctggttgtc ccagttcgag atctgtggct gatacaggag gatgcttccg
     1381 ccggcggcgt cgtagaaccg cggccatcca ccgtcgatgg gtgccgcagc ggcggccgga
     1441 actgccggct tcgagccggc cgccgccgga gtcgcgggct tcgccgcggg ttgttgtgca
     1501 cttggcgccg tcggctttgc cgtctgcgcc gccggagtct gagcgaccag caacggcgtc
     1561 agtggctgca ggaacagact gattgcggcc aggacagcgg ctgccgcatg tcgtcgtcgg
     1621 tgtttcttca ctctcgatcc tcctgactac tcgacccggc ttggcaggtt acgaggagag
     1681 ccacgcgcgt gccttggtga ggtcggcctc ggggaagtac tccaccggcg ccttacgcag
     1741 ccctgcgccg gcgaacatca tcgcctggtt cttccacttg tcgtccccga cgattgcgat
     1801 ctgctcgatg ctgtcgccgt gctgaatgaa gaacttgagg tcgttccagt cagagcgcgt
     1861 ttcccaccct tcgaatcccg acaggttgaa cagcagcttg accggcccca cccgccctat
     1921 ttcccccgcc agccgttgtt cgcatttctg aaactccgcc ttcttgacct gtccccacat
     1981 ctcgagaagg tagacgttct ttccttcagg cttcatatcg atcggcatga tccgttgtgc
     2041 cctcccaccg gccggccggg ggatcccacg caatatcgtg ggtcgcgccg tcagcgacgt
     2101 cggaaacgct ctgcaaacac cagcaagtgc tactgcaaga gcagggactg tgccgacgga
     2161 aaggtggttg cgaagtgtcg cgaacgacgc aacgatgctc ggttccgggc cgaatccatt
     2221 caccgtgtgg gaccagcggt ctccacgttg ccggagtgcc tccggaatga cggaccgttc
     2281 ccacatggtg atgtcgtcat gttcgctcgc tgcgcgcgct gctcgtgcgc gattcgaaac
     2341 ggcttgactg ttgcaaaacc agtctggaaa cgaaacctgt cgaccgaaag gaacagtcga
     2401 acatgaagaa gcaattcgtg gtgtctatgg tggctctcgt gctcgtcgtg cctgcgttgt
     2461 tgctggcgca gaagaccagc tacgacttcg acaagggcgc gaacttcgcg gcctacaaga
     2521 cctacgcgca caaggacgga accaaggtcg gccagccgct catcgacgac cgcatcacgt
     2581 cggctatcga ttcggcgatg gcgtcgaagg gattcaccaa gagcgacacg ccggacgtct
     2641 ttgtcgtcta ccacgtggcc tttgacaagc agaaggacat ctcgaccttc agttccggtt
     2701 acggcggcgg ctacgggccc tatgggtggg gttacggcgg cggttgggcc ggcggcacga
     2761 cgacgaccca ggtgcgggac atcctgatcg ggacgctcgt gatcgatctc gctgacgcgg
     2821 ccaagaagca gatggtgtgg cgcgggatgg cgaccaagga aggaatcaac ccgcagcaga
     2881 aaccggacaa gctggataag aacatcaata acgcagtcgc caagatcttc aagaactacc
     2941 cgccgaaaca gaagggctaa tcgaacgctg aatgttgaat gctgattgct gatcggtgtt
     3001 gattggttac cgggcatttt tcaatgagca ctgagccatc aaagatcagc atgcttccgg
     3061 agggcatgac ccgtggtgaa acggatcttc gcgacgatta cgttgttgat gctgtgcggc
     3121 ctggtcgtgc acgtgcaatc accggccgcg tctgacaagg agacgcgcac gaccaacctg
     3181 accgcgtaca ccgaactgct caggagcgat gtgcgcgcgg agaaggttgc catcctcacc
     3241 gaagtgatgg gatttaccga agccgaggac aaggccttct ggccaatcta tcgcgagtac
     3301 gatgccgaga tgtcgaagct gggcgacgag cgggtcgccc tgatcggaga cttcagcagc
     3361 cactacgagc agatgaccga cgcggcagcc gatcagattg cgacgaaggc gctcgatctc
     3421 gaggcacgac gccaggcggt caaagccaag tactacgatc gcgtcaagaa ggccttgtct
     3481 ccgaggaccg cgctccgcta cctgcaggtc gagcatcagc tgcagctgat catcgacctg
     3541 cagatctccg cggcgctgcc aatcgccaag taacaggagc tgagatgaaa taccgaccca
     3601 cccactggat caccgccgtg ctcgtcctgg ggatcggcgt gtctctgtcg gcggatcgcg
     3661 tcaagctgaa gtcgggcaag gtcgtggaag ggatgttcgt cggcgccgac agcaaggccg
     3721 tccggattct tctcgacacc ggcaaagtca ccgagatcgg gatcgatgac gcagtctccg
     3781 tcgagttcac gccgcgaaag ccaacgccac cgccaccgcc gccgcccaac cccgcgacgc
     3841 aaccgaagcc ggtccaggtg ccgtctggta cgccgatcaa cgtccggctc acgcaaggga
     3901 ttgatgtcga tgcctcgaag gcggggcagt cattcaaggc cattgtggac gatccggtga
     3961 tgatgaacgg gtcgatcgtg attccgcgcg gcgccaccgc ggagctccag gcggttgcgg
     4021 ttcagcagtc aggcaagatg aagggcagcg acaagatttc gctgaagatg aactccatcg
     4081 gctttggcgg caacgtctac caggtcacga ccgcgtacgt cgaaacgaaa ggcggcggcg
     4141 aaggcaagaa gacggcgcgc aaggtcggcg gcggggcggg tctcggcgcc atcgtcggcg
     4201 ggatcgcggg cggcgggtcg ggcgcggcca tcggtgccgc cgtcggcggc gccacaggcg
     4261 cggtggttgc cagcggcggc gaggaacacc tgaaactccc gg
//
LOCUS       DASSRL010000124         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_423181, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000124 DASSRL010000000
VERSION     DASSRL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423181"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..798
                     /locus_tag="VFL49_02650"
     CDS             127..798
                     /locus_tag="VFL49_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET7910904.1"
                     /translation="MAVAIAHHPREFVAIVMSTVAIFAIVINALFLQKGPHPAPIFAA
                     RPLLHQETTLPPRLQGTSSHLGSDLAAQARVQLIANIQRELTRKGFYDGAADGIWGSK
                     TDAAVRDFLQASGSKVNPEANENLLRAVSSYVAKPAIAAPSANAPSTDPIAKLIAPSK
                     RIVSIQRALADFGYGQIKATGVYDPETRNAIEKFERDHRLPVTGQISDRFVRELAAMT
                     GRPLE"
     gene            835..1185
                     /locus_tag="VFL49_02655"
     CDS             835..1185
                     /locus_tag="VFL49_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HET7910905.1"
                     /translation="MRLKAGIWVAAYLRRCNVEGVFAAVRRHGAEEAGAIFIKINRLD
                     GTGALYGPAPQSAFDEAQPADRIFVALVGRDMPASETDIEARLTKEIRFDPDLWVIEV
                     EDRGGRNFLDNIVQ"
     gene            complement(1194..2051)
                     /locus_tag="VFL49_02660"
     CDS             complement(1194..2051)
                     /locus_tag="VFL49_02660"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET7910906.1"
                     /translation="MANPSARVPVDSLVDLACRDGVDVRPTLVRVLTDLYVQRPLHSS
                     EEETQYVELVLGLIDSVDARTRTAVATKLRQYPKAPAAILRKLDSLAAAKPEQDESAE
                     DLLELFFSSAPEERRLILTNLDVVSEPTRRGPAPPEGELVRRLESAALRRNPAEFSRV
                     LTRALNVSPALAERITFDGSGEPVVVAAKVLGMKAEALQRILLFLNPAIGQSVRRIFE
                     LCRLYSELSSAAAERMLTIWRTSPVRLRSRYESVYWDDEQHSPRSGSTEPVRAGVKSN
                     VQSARINER"
BASE COUNT          423 a          626 c          626 g          428 t
ORIGIN      
        1 ccgagccggt caagcttgcg acggatgctc accaagccaa tccgtatgtc tcaaaagatt
       61 gggtgaagaa aagtgcctag acgatccacc cgtaggggtt ccgaaaattt cggcagagcc
      121 ggccggatgg cggtcgcgat cgcgcatcat ccgcgcgaat tcgtcgcgat tgtgatgtcg
      181 accgtcgcca ttttcgcaat cgtcatcaat gcgctgtttc ttcagaaggg cccgcacccg
      241 gcgccgatat ttgccgcccg tccgttgctc catcaggaga caacactccc gccgcggctt
      301 cagggcacgt cgtcccatct tggttcagat ctggcggctc aggcacgtgt ccagttgatc
      361 gcgaatattc aacgcgaatt gacccgcaag ggcttttatg acggggcggc cgacggcatt
      421 tggggctcga agaccgacgc cgcggtacgc gacttccttc aggcctccgg gtcgaaagtc
      481 aatccggaag caaacgagaa cctgttgcgc gccgtatcaa gctacgtcgc gaagcccgcg
      541 attgccgcgc catcggcgaa cgcgccatcc accgatccca tcgccaaatt aatcgcgcca
      601 tccaagcgca tcgtgtcgat acagcgggct cttgcagact ttggctacgg gcagatcaag
      661 gcgaccggcg tgtatgatcc ggaaacacgt aacgcaatcg aaaaattcga gcgcgatcac
      721 cgtctgccgg ttaccggtca gatctccgat cgttttgtgc gcgaattggc ggccatgacc
      781 ggccgcccgc tggagtgaat ggcctcgcgg ctgcggcccg tgctactgaa gccgatgcgc
      841 ttgaaagccg gaatatgggt ggcagcctat ttgcgtcgct gcaatgtcga gggtgtgttt
      901 gcggcagtac gccgacacgg cgccgaagag gccggcgcca tctttatcaa aataaatcgc
      961 ctggatggaa cgggtgcgct ctatggtccg gctccgcagt ccgcatttga cgaggcacag
     1021 ccggccgatc gcatttttgt cgcccttgtt gggcgtgata tgccagcatc cgaaacagac
     1081 atcgaagcac ggctgacgaa agaaatacgt tttgatccgg acctttgggt tatcgaagtt
     1141 gaggaccgtg gcggccgaaa ttttctcgac aatattgtgc aatagcggct cttctaccgc
     1201 tcgttgatcc gagcagattg cacgttcgac ttcacgccgg cacggaccgg ttccgtgctg
     1261 cctgaacgcg ggctgtgttg ctcatcgtcc caatagacgg actcgtatct cgaccgaagt
     1321 cgcacgggac tcgtccgcca gatggtcaac attcgctcgg cggcggctga actcagctcg
     1381 ctgtagagcc ggcacagctc gaaaatccgt cggaccgatt ggccgatggc cggatttagg
     1441 aacagcagga ttcgttgcag cgcttcggct ttcatgccga ggacttttgc cgcgacgacg
     1501 acaggttcac ctgagccatc gaatgtaatt cgctcggcga gcgccgggct gacattaaga
     1561 gcacgcgtaa ggacgcggct gaactcggca gggttccgtc gcaatgctgc gctttccagg
     1621 cgccggacca attccccttc cggcggcgct gggccgcgcc tcgtcggctc cgacacgaca
     1681 tcaaggtttg tgaggataag acgccgctct tcgggggccg atgagaaaaa caactcgagc
     1741 aagtcctcgg cgctttcgtc ctgttccggt ttggcggccg caagactgtc aagcttgcga
     1801 aggatggcgg ccggcgcttt cggatactgc cgtagctttg tggcgacagc ggtgcgcgtc
     1861 cttgcgtcga ccgaatcaat cagcccgagc acaagctcga cgtactgtgt ttcttcttcg
     1921 ctgctgtgca aaggtcgttg cacatacaga tcggtaagga cccgcaccag ggtcgggcgc
     1981 acatctacac cgtcacggca ggccaaatcg accaggctat cgacgggaac gcgcgcggaa
     2041 gggttcgcca tactcatgcg ccggcactac aggatgatcg acattagctg tcgctcgtta
     2101 gca
//
LOCUS       DASSRL010000125         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_668007, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000125 DASSRL010000000
VERSION     DASSRL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668007"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFL49_02665"
     CDS             <1..759
                     /locus_tag="VFL49_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HET7910907.1"
                     /translation="VATTMVVDGLLAFIVIWKRWEWPLWQTLLLISPFVVVDVVFFSA
                     NFLKLFEGAWAPILFGATMVLLILTWRRGTGILISKTRRTEVPLDTLFRNLEKKPPHI
                     VPGTAVFLTSDPEFAPTALLHNLKHNKVLHEHNVVLTIITADTPRVSEEERVQIKPLS
                     QHFARVSLKFGYMEQPNVPKALAIARKHGWQFDIMSTSFFLSRRALKPSAKSGMPSWQ
                     DHLFIGLARSASDATDFFQIPTGRVVEVGTQVTV"
     gene            1018..2865
                     /locus_tag="VFL49_02670"
                     /pseudo
     CDS             1018..2865
                     /locus_tag="VFL49_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
BASE COUNT          527 a          887 c          867 g          721 t
ORIGIN      
        1 gttgccacca ccatggtcgt ggacggactc cttgccttca tcgttatctg gaagcggtgg
       61 gagtggccgc tttggcagac cctgctgttg atatccccat ttgtcgttgt cgacgtcgtg
      121 ttcttctcgg cgaattttct caaattgttc gaaggcgcct gggcaccgat cttgttcggc
      181 gccacaatgg tcctcctgat tctgacctgg cggcgcggaa cgggaatcct gatcagcaag
      241 acccggcgca ctgaagttcc gctcgatacg ttgttccgca accttgagaa aaagcctccg
      301 cacatcgttc cgggaacggc cgtgtttctc acgagcgatc ccgaatttgc accgaccgcg
      361 cttctgcaca atctgaagca caacaaggtt ctgcatgagc acaacgtcgt cttgaccata
      421 attaccgccg atacgccgcg tgtttctgag gaggaacgcg tgcagatcaa gccgctgtcg
      481 caacacttcg cgcgggtgtc gctgaaattt ggctacatgg agcagcccaa cgtgccgaag
      541 gcattggcga tcgcccgcaa acacggctgg caattcgaca tcatgtcgac gtcatttttt
      601 ctctcgcgac gcgcgctcaa gccgtcggcg aagtccggca tgccgtcctg gcaggatcac
      661 ttgttcattg gcctggcgcg ctcggcgagc gatgcgacgg actttttcca gattccgacc
      721 ggtcgtgtgg tcgaggtcgg tacccaagta acggtctagg gcgcgcgctt tagcttttcc
      781 gtttaattta ttgatattgt ttgtatatca gtcatagccg tcagttgaat tctgcatgcc
      841 ttgccgggct gggttgcgta aatgatgctg ctgcggcgca acgtccagcg ttataagccc
      901 gcgcccttct tattgcagtg caacattcaa ggcagtccca tgtctcacag cattgccgat
      961 gcggccgctg aagcggctgt gtccgcaggt accaccgacg tgccggcaaa gatgcgtttc
     1021 tggtcgctca ccgtcggcag tattggcgtc gtctatggcg atatcggcac cagcccgctt
     1081 tatgcatttc gcgaggcgat atccgcggcg accggctcgg gcaatccggt aactgaactc
     1141 gtcgtcctcg gcattctttc gctgattatc tgggcgttgt tgttggtcgt cacagcgaaa
     1201 tacatcctca tcctgcttcg tgcggacaac aaaggcgagg gcggcacgct tgcgctgatg
     1261 gcactcgcat cgcattccct gctgcgccgg ggcggcacga tcatcttgct cggtattatc
     1321 agcggagcct tgttttatgg cgacgcaatc ataacgccgg ccttgtcggt gctgtcggca
     1381 gtcgaaggct tgaaggtcgt aacgccgacg ttcgatgcct atgtagtgcc tctcaccgtc
     1441 gtcattctcg tcgctctctt tgccgttcag tcgcgcggca ccgccagtgt cgcggcgttc
     1501 ttcggcccgg taatggtgct ttggtttgga gcaatcaccg ctgcaggcat ctggcacacc
     1561 gcccgcaacc cgatcgtatt gctcgcattc aatccgctgt acggcctcaa cttccttctg
     1621 gaacacggtc tcgttgcttt cttcacactc ggtgctgtct ttctcgtcgt caccggtgcg
     1681 gaggcgcttt acgccgatct tggccatttt ggccgggcgc ctattcgttt tgcctggctg
     1741 attgtcgtgc tgcccgcgct gactctcaac tatctggggc agggcgcatt ggtcctcgcc
     1801 aatccaaaaa cgatcgaaaa tcctttcttc ctgctttatc ccgagtgggc gcttctgccg
     1861 atggtgctgc ttgcgacgat cgcaaccgtc atcgccagcc aggcggtgat ttccggcgcc
     1921 tattcgctta cgcagcaggc catccaactc ggattgctgc cgcggctgga gatccggcat
     1981 acctcggaag ctctgttcgg gcagatctat atgcctcgcg tcaacacgtt gcttctgttc
     2041 ggcgtgctgc tgctggtgat gctgttccgg tcatcgagcg cgctggcatc ggcctacggt
     2101 atcgccgtga cggggacaat ggtcgtcacc gcgctgatgg caatcgtggt cgtgtggcgc
     2161 gtatggaact ggccactggc cgctgccttg gcgctgatcg tgccgttttt gtttatcgat
     2221 ctcacatttc tttcagcgaa ccttctgaaa atcatggaag gtgggtggat gccgctggcg
     2281 ctcggcgccg tagtgatgtc gatcatgtac acgtggcggc gcggcagccg gctcctgttc
     2341 gagaaaacgc gtcggctcga aacgccgctt gaggggcttg taaagagcct ggaaaagaaa
     2401 ccgccggcgc gcgtgccggg gacggcggtt ttcctgacca gcgatccggc cagcgctccg
     2461 accgcccttc tgcatagcct caagcactac aaagtgttgc acgaaaagaa tgtcatctta
     2521 accattgaaa ctgccggcac gccgcgcgtc gatgccggcg agcgagtccg tattcagcct
     2581 gttggcgaga cgttctcgcg ggtggtgctg cggttcggct tcatggaaac gccaaatgtt
     2641 ccgaaggcgc tcgccatcgc gcgcaagctc ggctggcaat ttgacatcat gtcgacgtca
     2701 ttcttccttt cacggcgcgc gctcaagccg gcgaaacaat ccgggatgcc gcgatggcag
     2761 gaccggctgt ttatcggcct tacgcgtgtc gcgaacgatg cgaccgatta tttccagatt
     2821 ccgaccgggc gcgttgtcga ggtcggcacg caggtcacaa tctagcgccc tcaaataggg
     2881 ctggtcggcc tgccgggatt ccggctaaaa ctccaaaaat cctgagtaat ggagtttcgg
     2941 catggctgac gaaatgcccc gcgccgacga ggtgacaaat ctcacgcctg aagaggttgc
     3001 tg
//
LOCUS       DASSRL010000126         8157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_965604, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000126 DASSRL010000000
VERSION     DASSRL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965604"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFL49_02675"
     CDS             <1..567
                     /locus_tag="VFL49_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET7910908.1"
                     /translation="IVMAMAKSVAMAAHGIEHSTVVTTMARNGTDFGIRVSSTGDAWF
                     TGPAQMARGLLFAGFTEADGNVDIGDSCITETVGLGGFAMAAAPAVVGFVGAGTLSDA
                     INYTRSMTEITVGPNPEWTLPTLSMLGVPTGIDILKVVSSGILPVINTAIANKLPGRG
                     QIGAGVVTPPMICFEQALIKFGEMNGAA"
     gene            570..2081
                     /gene="fdrA"
                     /locus_tag="VFL49_02680"
     CDS             570..2081
                     /gene="fdrA"
                     /locus_tag="VFL49_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HET7910909.1"
                     /translation="MSAVVNKVRRTFYMDSVALMRISRAVSSLTGVETAALMIGSATN
                     KKLMRDAGLLNADSDSAGPNDLIIAVRAGNEGAAGEAIKEAEKLLDAPAVSGGGGNEW
                     NPKTLATALQQLPGANLALISVPGEFAAAEARRALHNGLHVMMFSDNVPIEDEVALKR
                     EAKERGLLMMGPDCGTAIIGGAPLAFANVVPRGKIGIVAASGTGLQEISTLVARNGQG
                     VSHAIGVGGRDLKEAVGGITTLMGIDALDRDPGTEQIVLISKPPEPSVAKAVLERVGK
                     SKKKFSICFIGVENMQVPANASLSAELRTTAENAIGGKKIAWNGSPPSAADLIARVER
                     GRNKIQGLFSGGTMCAEAQVFLRRAGVPVASNVPIPGVKKPAAAASDAHVLLDLGDDE
                     YTVGRPHPMIDPTLRNQMIGEALKDGKTAVILLDVVLGYGANADPAGELIAQLAAAKD
                     RKALLITSVCGTEEDPQGYSRQVRALEDAGIVVAPSNAHAAELAIAVQRQISS"
     gene            complement(2078..3016)
                     /locus_tag="VFL49_02685"
     CDS             complement(2078..3016)
                     /locus_tag="VFL49_02685"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HET7910910.1"
                     /translation="MLNLETAIQHDKAIIVVVLGKIAHAIMRAHTEGAVIAVFRRSFY
                     VRFGDDVVCVGPPGLGKGPLNVLAAVPAGLEWPMVGLARQQSVLRSGSMLSIGNHLRF
                     DLNDAEVWQPPPAREFSRSDLQDGLHLLAESAQRRAPGGLGALLTTLGKPSLPTEAKD
                     NVLLQAALWSIAAVWQWLGVALAGSPEPPPPADNLVALGPGLTPSCDDFLCGVMAALN
                     YLGHRDIANRLAASVLPVAERETSLISAAYLRCAAKGHASAVLYDALESVLGGGRDLE
                     ARLDVVHAVGHTSGWDSLVGAASVCAALTQQTATTG"
     gene            complement(3028..3990)
                     /locus_tag="VFL49_02690"
     CDS             complement(3028..3990)
                     /locus_tag="VFL49_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET7910911.1"
                     /translation="MPIKDEIPSRLVIAIGGNATHPEGIRGTPSEQADVAAGLGRALL
                     PLMELNTELIITHGNGPVVGKILMRQSLARQRVEPMTLDICVAHSQGGIAYLLMQALE
                     NALRAVDNPRHVVCLLTQVEVDPDDPAFKNPSKPIGYFYSEEEAQALTAELGWDMRED
                     SGRGWRHVVPSPQPKHICDISLIQVVAKSGAVVIAGGGGGVPVVRTGKRQRHGVEAVI
                     DKDRTSALMANVLKIDDLMILTAVPRVAINFGKPDQKALERVTLSEIRKYHADGHFPP
                     GSMGPKIDAAIQFLEGGGKRVMIGRLEDVLPVLRGQTGTHIVAD"
     gene            4166..5410
                     /locus_tag="VFL49_02695"
     CDS             4166..5410
                     /locus_tag="VFL49_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET7910912.1"
                     /translation="MEKIELANKETVRRILQGEPVLIDVKPAGELIKGLTRNMILHAG
                     PPITWDRMCGPMRGAVCGIAVFEGWAKDLAEAEAAAAGGKYAFHPNHHFGAVGPMTGM
                     TTMSQPVMIVENKTFGNRAYCTVNEGLGKVMRFGGNDAEVLNRLRMIATVLGPALSAA
                     LKASGGIPLKSIIARGLAMGDEMHQRNVACSALFLREIAPFLAQTSTDSRKLADVLSF
                     IGKNDQFFLNIAMAMGKAMTDPANGVEGSSIVTAMCRNGTDFGVRVSGTGERWYTAPV
                     EMPTGLYFPGYSEKDANPDMGDSAIVETVGLGGFAMATATAVAGFIGAGSASEAANFT
                     KAMYEITTVRNPDWTMPNLDYAGVPTGIDIRKVLQTGTAPVINTGIAHKQPGVGQVGA
                     GVVRAPLACFERGLEGLAETLG"
     gene            5498..6241
                     /locus_tag="VFL49_02700"
     CDS             5498..6241
                     /locus_tag="VFL49_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET7910913.1"
                     /translation="MRRGEDASIYPPREPQSRTLANEVFEKLRADILSNSLPPGMKLR
                     FDDLRDAYKVGLSPLREALSRLAENRLVVATGQRGFRVPSVSVRDILDIAMVRKEVEG
                     LALRLSIQNGDDAWEARVVAARHKVTLLEKAGKNVAEDIWESRHREFHHTLVSACQSE
                     CLLHLHGLLCDQFDRYRRLSAQSRLPNSPRSLIHQQILEAALKRNADLAVRLLGEHID
                     KAAQLIVAGLSPPEVPRGPDRKPKRGKRA"
     gene            6246..7703
                     /locus_tag="VFL49_02705"
     CDS             6246..7703
                     /locus_tag="VFL49_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7910914.1"
                     /translation="MAQSTAFSGRLLDLSHRDRHSCFGAELQVSGTTFSKILPRQQNA
                     QHGDLVMPPFSNAHDHVRGTRPISIGAFDLPLELWLVYMTRLPPVDPYLVAAAALGRQ
                     ALGGIGTIMIHYTRPQNPNALGEELEVVAKAATDIGVRVSIAVAMRDQNPLGYGPTEK
                     LLDGLEPADKDAIRAKLLSAPQSPQNTVQFVDDLAAKIESPLVTVQYGPYGAEWCSRP
                     LLELIAKKSALTGRRVHMHLLESRVQREYLDHIYPDGVIKYLDSIGLLSQRLSVAHGV
                     WLRPDELELLAGRGVTVSVNSSSNLSIRSGMSPMRKMHELGVPFAMGLDGFSVDDDDD
                     AFRELRLNYLLHQGLGLEDGVPLPKLLHSSCYGGRFSVTGIEAGAGMAAGEPADLMVI
                     DYSRITKDVFMPLDEAALLARRATSNHLKQLVVAGRTIVRDGSLTGIALDQIQDELNS
                     QVRDGMSRNRPWQHISDRFRENLKVFYSSGLHRCG"
     gene            complement(7706..>8157)
                     /locus_tag="VFL49_02710"
     CDS             complement(7706..>8157)
                     /locus_tag="VFL49_02710"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910915.1"
                     /translation="ALNLDPEKEFRWLYYSDQGLDLYSNGVMVSRQLIKEKPQAVKGL
                     LRAIAKSIQETLSNPDAAIELLAKSQPLINKDIEKRRLLYVTRTLIDTPESREIGVGD
                     INDKRMADAIGVVVASYNLPRTPAVSEVFDRSFLPPIADRRLPKLTN"
BASE COUNT         1598 a         2540 c         2541 g         1478 t
ORIGIN      
        1 atcgtcatgg caatggcgaa gtcggttgcc atggcggcgc acggcatcga acattcgacc
       61 gtcgtcacca cgatggcacg caatggaacc gatttcggca ttcgcgtgag ctcgaccggc
      121 gacgcctggt tcaccggacc ggcgcagatg gcgcgcgggc tcttgttcgc gggcttcacc
      181 gaagccgacg gcaatgtcga tatcggcgat tcctgcatca ccgagacagt cgggcttggc
      241 ggctttgcga tggcggcggc gccggcggtg gtcggcttcg tcggcgcggg cacgctcagc
      301 gatgcgatca actacaccag gagcatgacc gagattaccg tcgggcccaa tccggaatgg
      361 acgctgccga cgctgtccat gctgggcgtt ccgaccggga tcgacatcct caaggtcgtg
      421 tcttcgggaa tactgccggt catcaacacg gcgattgcca ataagctgcc gggccgcggc
      481 cagatcggcg ccggcgtggt gaccccgccg atgatctgct tcgagcaggc gttgataaag
      541 ttcggcgaaa tgaatggagc ggcgtaagca tgagtgcggt cgtcaataaa gttcggcgca
      601 cgttctatat ggattccgtt gcgctgatgc gcatctcgcg cgcggtgtca tccctcaccg
      661 gtgtcgaaac cgccgcactg atgatcggct cggccaccaa caagaagctg atgcgcgatg
      721 ccggtctgct caacgccgac agcgacagcg ccggccccaa cgatctcatc atcgcggtgc
      781 gggccggcaa cgagggtgcg gcgggagaag caatcaagga agcggaaaaa ttgctcgatg
      841 cgccggccgt gtcgggcggc ggcggcaacg aatggaatcc aaagacgctt gcgaccgcgc
      901 tgcagcagct tccgggtgcc aatctcgcgc tgatctcggt gccgggcgaa ttcgccgcgg
      961 cggaagcgcg tcgcgcgctc cataacgggc tgcacgtgat gatgttcagt gacaacgtcc
     1021 cgatcgaaga cgaagtcgcg ctcaagcgcg aggcgaagga gcgcggcctg ctgatgatgg
     1081 gacctgattg cggcacggcg atcatcggcg gcgcgccgct cgcattcgcc aacgtcgtgc
     1141 cgcgcggcaa gatcgggatc gttgcggctt ccggcaccgg cttgcaggag atctccacgc
     1201 ttgtcgcgcg caacggccag ggcgtttcgc acgcgatcgg cgtcggcggc cgcgacctca
     1261 aggaagcagt cggcggcatc acgacactca tgggcatcga tgcgctcgac cgcgaccccg
     1321 gcaccgagca gatcgtgctt atctcaaagc cgcccgagcc atcggtcgca aaagcggtgc
     1381 ttgaacgtgt cggcaagagc aaaaagaaat tctcgatctg cttcatcggc gtcgagaaca
     1441 tgcaggtccc cgcgaacgcc tcgctgtcgg cggaactgcg gacgaccgcg gaaaacgcga
     1501 ttggtggcaa gaagatcgcc tggaacggct cgccgccgtc cgccgccgat ctgattgcgc
     1561 gtgtcgagcg tgggcgcaat aaaattcagg gcctgttctc cggcggcacg atgtgcgcgg
     1621 aagcgcaggt tttcctgcgc cgggccggcg tgccggtcgc gtcgaacgtg ccgattcccg
     1681 gtgtgaagaa gccggcagcc gccgcaagcg acgcgcatgt gctgctcgat cttggcgatg
     1741 acgagtacac ggtcggccgc ccgcatccga tgatcgatcc gacgctgcgc aaccagatga
     1801 ttggcgaagc gctcaaggac ggaaaaaccg ccgtgatcct gctcgacgtc gtgctcggtt
     1861 acggcgccaa cgccgacccg gcgggcgaat tgatcgcaca attggcagcg gcgaaggacc
     1921 ggaaagcatt gctgattacg tcggtgtgcg gcaccgagga agaccctcag ggctattcgc
     1981 gccaggtacg ggcgctcgaa gacgcaggca tcgtcgtcgc gccgtcgaat gcgcacgcgg
     2041 ccgagcttgc gatcgctgtt cagcggcaga tttcgtccta gccggtggtt gcggtttgct
     2101 gcgtcagcgc ggcgcatacg ctcgccgcgc cgacgagact gtcccaaccg gatgtgtgcc
     2161 caacggcatg cacgacatcg agccgcgctt ccagatcacg gccgccgccc aaaacacttt
     2221 ccagcgcatc gtacagcacg gccgaagcat gtcctttggc cgcgcaccgc agataggccg
     2281 cgctgataag gctcgtctcc cgctccgcaa cgggcaggac cgacgcggca agccggttcg
     2341 cgatatctcg atgtccaaga tagttcagcg ccgccatgac gccgcaaagg aaatcatcgc
     2401 acgacggcgt cagacccggc cccagcgcaa ccagattgtc ggcaggtggc ggcggttcgg
     2461 gagaacctgc cagcgctacc ccaagccatt gccagacagc ggcgatggac caaagcgcgg
     2521 cttgcaacaa gacattgtct ttcgcctcag tcggcaatga cggtttgccc agcgtggtca
     2581 gcagcgcgcc cagaccgccc ggagcacggc gctgcgccga ctccgccagc aaatgcaaac
     2641 cgtcctgcaa atccgatcgc gaaaattcgc gcgccggcgg cggttgccac acctcggcgt
     2701 cgttcaaatc gaaacgcaga tggttgccga tggacagcat cgaccccgat cgcaaaacag
     2761 actgctggcg cgcgagacca accatgggcc actcgaggcc ggccgggaca gccgccagga
     2821 cgttcagcgg ccccttcccg aggcccggcg gaccgacaca gaccacgtcg tcaccgaacc
     2881 ggacataaaa actgcgacgg aataccgcga taacggcgcc ttcggtatga gcgcgcatga
     2941 tcgcatgcgc aatcttaccg agcacgacga cgatgatcgc cttgtcgtgc tgaatcgctg
     3001 tttccaaatt gagcaacgcg ctctccgcta atcggcgacg atatgcgttc cggtctgccc
     3061 gcggaggacg ggcaacacgt cttccaaacg tccgatcatc acccgcttgc cacccccttc
     3121 gagaaactgg atcgccgcat cgatcttcgg ccccatgctg cccggcggga agtgcccgtc
     3181 cgcgtgatac ttcctgattt ccgacaacgt cacccgctcg agcgccttct gatccggctt
     3241 gccgaaattg atcgcgacgc gcggcaccgc cgtcagaatc atcaaatcat cgattttcag
     3301 cacgttcgcc atcagcgccg aggtgcggtc cttgtcgatc accgcttcaa cgccgtgccg
     3361 ctgccgcttg ccggtccgca caaccggcac accgccgccg cctccggcga tcacgaccgc
     3421 gccgctcttg gcgacgacct gaatgagtga gatgtcgcag atgtgcttgg gttgcggcga
     3481 cggaacgacg tgacgccagc cgcggccgga atcctcccgc atatcccaac cgagctccgc
     3541 tgtgagcgcc tgtgcttcct cttcgctgta aaaataaccg atcggtttgg acggattttt
     3601 gaacgccgga tcgtccgggt cgacctcgac ctgcgtgaga agacagacga cgtggcgcgg
     3661 gttgtcgacg gcgcgcagcg cgttctccaa agcctgcatc agcagatagg cgatgccacc
     3721 ctggctgtgc gcgacgcaga tatcgagcgt catcggctcg acgcgctggc gcgccagcga
     3781 ttggcgcatg agaattttgc ctacaaccgg accgttgccg tgcgtgatga tgagctccgt
     3841 attgagctcc atcagcggca gcagcgccct gcccagtccg gcggcgacgt ccgcctgctc
     3901 gctcggcgtg ccgcggatgc cttccggatg cgtggcgttg ccgccgatcg caatgacaag
     3961 gcggctcgga atctcgtctt tgatgggcat agccgaatcc ccggctgcag agacaaagtc
     4021 taagcgatcg atccagtcat tgcaaatttt tcgaaattcc tggccttcgc gtatataatg
     4081 atgttcttcg atcttattgc cgaactgcgg tcgatcccat atggtgcgga tcaatcacgg
     4141 acggcggtcg gcaggagagt tgacgttgga gaaaatcgag ctcgcgaaca aggaaaccgt
     4201 acgaagaatt ctacagggcg agccggttct catcgacgta aagcccgccg gcgagctgat
     4261 caaaggcctc accaggaaca tgatcctgca tgccgggcct cccatcacct gggatcgcat
     4321 gtgcggcccg atgcgcggcg cggtctgcgg catcgcagtg ttcgagggct gggcgaagga
     4381 ccttgcggaa gccgaagccg cggccgccgg cggcaagtac gctttccatc ccaatcatca
     4441 cttcggcgcc gtgggcccga tgaccggcat gacgacgatg agccagccgg tgatgatcgt
     4501 cgagaacaag actttcggca accgcgccta ctgcaccgtc aacgaaggcc tgggcaaggt
     4561 gatgcgcttc ggcggcaacg atgccgaggt gctcaaccgg ctgcggatga ttgcaaccgt
     4621 gctcggccct gctttgtccg cggcgctgaa agcaagcggc ggcattccgc tgaaaagcat
     4681 catcgcgcgc gggctggcga tgggcgacga aatgcatcag cgcaacgtcg cctgctcggc
     4741 tttgttcctg cgcgagatcg cgcccttcct ggcgcaaaca tcgacggatt ccaggaagct
     4801 cgccgacgtc ttgagcttca tcggcaagaa cgaccagttc ttcctcaaca tcgccatggc
     4861 gatgggcaag gcgatgaccg atccggctaa cggagtcgag ggctcctcaa tcgtcaccgc
     4921 gatgtgccgc aacggtacgg acttcggcgt gcgggtcagc ggcaccgggg aacgctggta
     4981 caccgcgccg gttgaaatgc cgaccgggct ctatttccct ggttattccg agaaggatgc
     5041 caatcctgac atgggcgatt ccgcgattgt cgaaactgtc gggctcggcg gcttcgcgat
     5101 ggccaccgca acggcagtgg cgggtttcat cggcgccgga tcggcgagcg aagccgcgaa
     5161 tttcaccaag gcgatgtacg agattacgac cgtgcgcaat cccgactgga ccatgcccaa
     5221 tctcgattat gccggcgttc cgaccggaat cgacatccgc aaggtgctgc agaccggcac
     5281 cgcgccggtc atcaacaccg gcatcgcaca taaacagccg ggcgtcggcc aggtcggcgc
     5341 cggcgtcgtg cgcgcgcccc ttgcctgctt cgagcgcggc ctcgaagggt tggcagagac
     5401 cttgggatag tggctggaat ttgcgccctg ccgcttgggg ttcggtaaaa caggtgcccg
     5461 catgaagaga gccagcgtac aggagcggac agcgagcatg agacggggcg aagacgcgtc
     5521 gatctatccg ccgcgcgagc cgcaatcgcg cacgctggct aatgaagtat tcgagaagct
     5581 gcgcgcggac atcctgagca actctttgcc accggggatg aaattgcgct tcgacgactt
     5641 gcgcgacgct tataaggtcg gcttgagccc tttgcgtgag gcgctgtcgc gccttgcgga
     5701 aaaccgtctt gtggtcgcca cggggcagcg cggatttcgc gtgccatccg tatctgtgcg
     5761 cgacatcctc gacattgcga tggtacgcaa ggaagtggaa gggctggcgc tgcgcctgtc
     5821 gatccagaac ggcgatgatg cctgggaagc gcgggtggtc gcggcgcgcc acaaggtaac
     5881 gctgctggaa aaggccggga agaacgtcgc ggaagacatc tgggaaagcc ggcaccgtga
     5941 attccaccac acgctcgtgt cggcgtgcca atccgagtgc ctgctgcacc tgcacggcct
     6001 gctttgcgac caattcgacc gctaccgccg cctgtcggcg caaagccggc tgccgaactc
     6061 accgcgctcc ctgatccacc agcagatcct cgaggcggcg ctcaagcgaa acgccgacct
     6121 tgcggttcgc ttgctcggcg agcacatcga caaagcagcc caattgattg ttgctggcct
     6181 gtcgccgcct gaagtgccgc gcggtccgga cagaaaacca aagcgcggca aacgcgcgta
     6241 agtctatggc ccaatcgacg gcattcagtg gccggcttct cgatctgtcc catcgcgacc
     6301 ggcactcctg tttcggcgcc gagttgcagg tttccggaac gaccttttcc aaaatcttac
     6361 cccggcagca aaacgcgcag cacggcgatc tcgtcatgcc gcctttcagc aacgctcacg
     6421 atcatgtccg gggaacacgt cccatttcga tcggagcgtt cgatctgccg ctcgagctgt
     6481 ggctggttta catgaccagg ctgccgccgg tggatccata tcttgttgcg gccgccgcgc
     6541 tgggccgtca ggcgctgggc ggcatcggca ccatcatgat ccattacacg cggccgcaga
     6601 atcccaatgc cctcggcgaa gagctggaag tcgttgccaa agccgcgacc gacatcggcg
     6661 ttcgtgtctc catcgccgtc gcgatgagag atcaaaatcc gctcggctat ggtccgactg
     6721 aaaagctgct cgacgggctg gagccggctg acaaggatgc cattcgcgcc aagctgctgt
     6781 cggcgccgca atcgccgcaa aacacggttc aattcgtcga tgacctggcg gcaaaaatcg
     6841 aatccccgct cgtaacggtt caatacggcc cctatggcgc cgagtggtgc tcaaggccgt
     6901 tgctcgagtt gatcgccaag aaatcggcgc tcaccgggcg ccgcgtgcat atgcatctgc
     6961 ttgagtcgcg cgtccagcgg gaatatctcg accatatcta cccggacgga gtcatcaaat
     7021 acctcgacag cattggtctg ctgtcgcaac ggctgtcggt ggcgcacggc gtatggctgc
     7081 ggccggacga attggaattg ttggccgggc gcggcgtgac ggtgagtgtc aactcaagtt
     7141 cgaatctgtc gatacggtcg ggcatgtcgc cgatgcggaa aatgcacgag ctgggcgttc
     7201 cgtttgccat ggggctggac ggattcagcg tcgacgatga tgacgacgcg tttcgcgaat
     7261 tgcgactgaa ctaccttctg catcaggggc tcggcctgga ggacggcgtc ccacttccga
     7321 agctgcttca ttcgagttgt tatggcgggc gcttcagcgt cacaggaatc gaggcaggcg
     7381 ccggaatggc agccggcgaa ccggctgatt tgatggttat cgactattcc aggatcacga
     7441 aagacgtttt catgccactc gacgaagcgg cacttttggc tcggcgcgcg acgtcaaatc
     7501 acctgaagca actcgtcgtg gcgggccgga ccattgtccg cgacgggtcc ttgaccggaa
     7561 ttgctcttga tcagattcaa gatgagctga attcccaggt acgtgacggc atgtcacgca
     7621 acaggccctg gcaacacatc tcggaccgct tcagagaaaa tctgaaagtc ttttattcca
     7681 gcggactgca ccgctgcggc tgatatcagt tcgtgagctt cggcagcctc cggtccgcaa
     7741 ttggcggcaa aaaggaacgg tcgaaaactt ccgagacggc cggcgtgcgc ggcaggttat
     7801 aggatgccac gacaacgccg atggcatcgg ccatgcgctt gtcgttgatg tcgccgacac
     7861 cgatttcgcg gctctcaggc gtatcgatca acgttctcgt cacatacagc agccgccgct
     7921 tttcgatgtc cttattgatc aacggttgcg acttcgccag caattcgatc gccgcgtcgg
     7981 ggttcgaaag ggtttcctgg atcgatttgg cgatggcccg cagcaggcct ttgacggcct
     8041 ggggcttttc tttgatgagc tgcctggaga ccatcacgcc gttcgaataa agatcaaggc
     8101 cctggtcact atagtagagc caccgaaatt ccttttcagg atcgagattg agagcga
//
LOCUS       DASSRL010000127         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1613272, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000127 DASSRL010000000
VERSION     DASSRL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613272"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFL49_02715"
     CDS             <1..849
                     /locus_tag="VFL49_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HET7910916.1"
                     /translation="NTGHVEAINATGLRIIGKTARTADIQAISDPVALKGSAFDAIIF
                     LIKSKTTGAALAQLRPMLAGNPMLVTLQNGMGNAEVLLAAPNATVVRGVTMNAGRYVG
                     PGRIECLVEGKSWLGPARGSVEDVRPLADLLSKAGMETEIVADPMGAVWSKFVFNCVM
                     NPLGALMMGDNAARYDSAEMRALIDDMAAECEAVVRALGGTFAFPPMDFVEKVRSGQI
                     PISRHAGSMALDIARGAPTEIDELTGFVVREGERLGVPVPNCRAVYRLVKGLELAAQR
                     RVANPR"
     gene            984..1529
                     /locus_tag="VFL49_02720"
     CDS             984..1529
                     /locus_tag="VFL49_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7910917.1"
                     /translation="MDDSDEALMARVARGDERAFQLLARRHVPAMLGIARRILGNAAE
                     AEDVAQEALMRVWTHAPRWQPLALFRTWLTRVVVNLCLDRKRRAPWVELEAAGDIVDP
                     SPGATEKVETEERERMVAAAIDRLPARQRSAIMLTYGEGMSNAEVAEILETSVSAVET
                     LLVRGKQNLRRALGNMIDEDK"
     gene            1531..1881
                     /locus_tag="VFL49_02725"
     CDS             1531..1881
                     /locus_tag="VFL49_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910918.1"
                     /translation="MNNEQFDKLIGSAIKRRAAKPADEAAINRVLRRVGAPLPRQKRP
                     LWRLPAVLLDWQFAPAWPRMAALACCAALGFYVGIAGFDRRFDDLAAAPAFVGGGGIG
                     SITFEPEAFTGARP"
     gene            1878..>2282
                     /locus_tag="VFL49_02730"
     CDS             1878..>2282
                     /locus_tag="VFL49_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET7910919.1"
                     /translation="MSIAHALPGATHGSPRWLLLSSLALNLFFVGVALAMVIRAPAPS
                     YWDPNVFVRVERLAATLPPADAGILRGQINANHAAIEDAQAKYHSARRHIHEVLRQEP
                     FDVEAMRAATEQARIARLNFDQTIQGVFADIAA"
BASE COUNT          420 a          712 c          735 g          415 t
ORIGIN      
        1 aacaccggac acgtcgaggc tatcaatgca accggcctgc ggatcatcgg caagactgcc
       61 cggaccgccg atattcaggc aatctcggat ccggtagcgc tgaaaggttc ggcattcgat
      121 gccattatct tcctgatcaa atccaagacg accggtgccg cgctcgccca attacggcca
      181 atgctcgccg gcaatccgat gctggtgacg ctgcagaacg gcatgggcaa cgcagaggtt
      241 ttgctcgccg ctcccaacgc aaccgtcgtc cgcggtgtaa cgatgaatgc cgggcgttat
      301 gtcgggccgg gtcgcatcga atgccttgtc gaagggaaga gctggctcgg tccggcgcgc
      361 ggcagcgtcg aagacgtgcg tccgctcgcc gacctcctca gcaaggccgg catggagacg
      421 gaaatcgtcg ccgatccgat gggcgcggtc tggtccaaat tcgtcttcaa ctgcgtgatg
      481 aacccgctgg gcgcgctgat gatgggcgac aacgcggccc ggtatgattc agccgaaatg
      541 cgcgccctga tcgatgatat ggccgccgaa tgcgaggccg tagtgcgggc actgggcggg
      601 accttcgcgt tcccgccgat ggacttcgtc gaaaaggttc gctccggcca aatcccgata
      661 tccaggcacg ccggctccat ggccctcgat atcgcacgcg gcgcgccgac ggagatcgac
      721 gaattgaccg gatttgttgt gcgtgagggc gaacggctgg gagtgccggt cccgaactgc
      781 agggccgttt accggctggt gaagggactt gagcttgcgg cccaaaggcg ggtggcgaac
      841 ccccgataat tcaacacgtt aggcgtaatc gacgggctgt tttcccgata tactgccgcc
      901 acaggattcg gacccgccac tcgttaaaca gacaggccaa tcttcaatga acgggtatcc
      961 gctatggcaa ccgccgcatc gcgatggatg acagcgacga ggcgctcatg gcccgcgtcg
     1021 ctcgcggcga cgaacgggca ttccagttgc ttgcgcgccg tcacgtgccg gccatgctcg
     1081 ggattgcccg gcgcattctc ggaaatgctg cagaagccga ggacgtggcg caggaagcgc
     1141 tcatgcgggt gtggacgcat gcgccgcgtt ggcagccgct tgcattattc cgcacctggc
     1201 tcacccgcgt ggtggttaat ctctgtctcg accgcaagcg gcgggcacca tgggtcgagt
     1261 tggaggcggc cggcgacatt gtcgacccgt cgccaggcgc aaccgagaaa gttgagaccg
     1321 aagaacgcga acgcatggtt gcggcggcca tcgacaggct gcccgcacgc cagcgcagcg
     1381 cgatcatgct cacctatggc gaaggcatga gcaatgcgga ggtcgccgaa attcttgaga
     1441 cgtcggtatc ggcggtcgag accttgctgg tgcgcggcaa gcagaatttg cgccgcgcgc
     1501 ttggcaacat gatcgatgag gataagtgag atgaacaacg agcaatttga caagcttatc
     1561 ggatcggcaa tcaagcgccg ggcagcgaag cccgccgacg aggctgcgat caatcgcgtg
     1621 ctcagacgcg tcggcgcccc gttgccgcgg caaaagcgac cgctttggcg tctgcccgcg
     1681 gtgctgctcg actggcagtt cgcgcctgcc tggccacgca tggccgcgct ggcctgttgc
     1741 gctgcgctcg gattctatgt cggtatcgcc gggttcgacc gtcgctttga cgacctggcg
     1801 gccgcgccgg cctttgtcgg cggcggcggc attggctcaa ttaccttcga acccgaagca
     1861 ttcacgggag cgcgtccatg agcatcgccc atgcactgcc gggtgccacg cacggttcgc
     1921 cgcgctggct gttgctaagt tcgctcgcgc tcaacttgtt cttcgtcggc gtcgcgcttg
     1981 cgatggtcat ccgggcaccg gcgccttcct attgggaccc caacgtgttc gtccgcgtcg
     2041 aacgcctggc ggcaaccttg ccgccggccg atgccgggat attgcgcggt cagatcaatg
     2101 ccaaccatgc agccattgag gacgcgcagg ccaaatatca ttcggcgcgt cgccacattc
     2161 atgaggtctt gcggcaggaa cccttcgacg tcgaggcgat gcgcgccgcc accgagcagg
     2221 cacgcatcgc gcgactgaat ttcgatcaga cgatccaagg cgtctttgcc gacatcgcgg
     2281 cg
//
LOCUS       DASSRL010000128         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1578168, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000128 DASSRL010000000
VERSION     DASSRL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578168"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFL49_02735"
     CDS             complement(<1..465)
                     /locus_tag="VFL49_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910920.1"
                     /translation="MSRTHNSGALSRRNLLQAGAGLAGGALLPQAFTLPAFAVGEQPP
                     IGTWPEGSSGNSVTVSIAVPRTGTYAVQGEDELKGWQLAVEHMNSGHELMKKFSPKTK
                     KGVLGKEVKIVVADSGAKPNTAVQAHQRAITENKAVLLTGSTSSAVAVATNKL"
     gene            916..1818
                     /locus_tag="VFL49_02740"
     CDS             916..1818
                     /locus_tag="VFL49_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7910921.1"
                     /translation="MLEKLEFMIALSREQHFGRAAESCGVAQPTLSLGIQSLEQILNT
                     PLVKRASRFQGFTPEGERVLLWAHRLVGDARAMRQDILGLKNGAESHLRIAALPSAMP
                     LVAKLTVPLQMRHPNIRFTILGRTSTVLLNMLRQREIDAGVTYLSNEPIGEVSAVPIY
                     REEYLLLTTVKGPLGDADRVTWSQAAEAPLCLLGRDLQSRRIVDSILRRIGIETKPLL
                     ETDSLSALMAHVRVGEWASIIPNSALEDVELTDTLRAISIVEPEVFHTIGLVVSDRYP
                     VQPAVTSLIEEARTRAPRGLLPAA"
     gene            1970..>2565
                     /locus_tag="VFL49_02745"
     CDS             1970..>2565
                     /locus_tag="VFL49_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7910922.1"
                     /translation="MGLLSFLDREHTVAKPGYSRWMVPPAALCVHLCIGQAYAYSVFN
                     LPMTKLIGISQSAPNDWKLTQLGWIFSIAIFFLGVSSAVFGRWVEEGGPRRAMFTAAL
                     CWAGGFFISAFGVYAHTLWIIYLGYGVLGGIALGIGYISPVSTLIKWFPDRPGMATGM
                     AIMGFGGGAFIASPLSVWLMGKFTTATHIGVAETFIVLG"
BASE COUNT          507 a          763 c          715 g          580 t
ORIGIN      
        1 cagcttgttg gtcgcaaccg cgaccgccga ggaggtcgag cccgtgagaa gcacggcctt
       61 gttctctgtt atggcgcgct ggtgggcctg cacggcggtg tttggcttgg cgcccgagtc
      121 ggcaacaaca attttcactt ccttgccgag cacacctttc ttggtcttcg gcgagaattt
      181 cttcatcagc tcgtgaccgg agttcatatg ctcgaccgcg agctgccaac ccttgagttc
      241 gtcttcgcct tgcacggcat aagtgccggt gcgcggcact gcgatgctga cggtcaccga
      301 attgcctgac gagccttccg gccaggttcc gatcggcggc tgttcgccaa ctgcaaaagc
      361 cggaagcgta aacgcctgcg gcaacagcgc gccgcccgca agaccggcgc ccgcctgtag
      421 caagttccgg cgcgacagcg cgccggagtt atgggtgcgt gacattttta tttcctccct
      481 ttgaaaacca attcgagatc gcccccgaat aattttcggc cttgtcgttg ctccaccgcc
      541 aatgcacgcg tctcaatcgc ggcataacga ttcaagccgc cgaataccat tccgaaagat
      601 cattgccgac gcgattggca ttttggtcaa tgccactttc gtcgatgcag cggtgaataa
      661 ggtcttcgga ctaaccgatt ggcgcgagcg cgcacggccg caacataaac ttttggacga
      721 tggtcgcgac caacgataag tgtaataaat tacatcatta tacttttaaa ttaaccgagt
      781 gcagcgagac gtgcgccgct taaggcatgg aaaaagccta gattccgcgc ctttaccgcg
      841 tcgatacgga tgaataggct ggcgtctgca aatatccgcg ccataaatat cggcaacatc
      901 agggcgagac aggcaatgct cgaaaagctg gaattcatga tcgcgctgtc aagggaacag
      961 catttcggac gtgccgcaga aagctgcgga gttgcgcagc cgactctctc actcggcatc
     1021 cagagccttg agcagatcct caatacgccg ctggtgaagc gggcttcgcg atttcaaggc
     1081 ttcactcccg aaggcgagcg cgtcttgctg tgggcacatc gcctcgtcgg cgatgcgcgc
     1141 gcaatgcgcc aggatattct cggcttgaag aacggcgcgg aatcccattt gcgcattgcc
     1201 gcgcttccat ccgcgatgcc gctcgtcgcc aagctcactg ttcctttgca gatgcgtcat
     1261 ccgaatattc gcttcacaat tcttggccgc acctcgaccg tgcttctgaa catgttgcgg
     1321 cagcgcgaga ttgacgcagg cgtcacctat ttgagcaacg agcctattgg agaggtcagc
     1381 gcggtcccga tttatcgcga ggaatatctg ttgcttacga cagttaaggg tccacttggc
     1441 gatgcggatc gtgtcacctg gtcacaggcg gccgaagctc ccctgtgcct gctcggccgc
     1501 gacctccaga gccgccgcat cgttgacagc attcttcggc gcatcggcat agagacgaag
     1561 ccactgctgg aaaccgattc gctgagcgca ctgatggcac atgtacgcgt cggcgaatgg
     1621 gcgagcatca tcccgaattc ggcactcgaa gacgttgaat tgaccgatac cttgcgtgcc
     1681 atttcgatcg tcgaacctga ggttttccac acgattggac tggtcgtctc tgaccgctac
     1741 ccggtccagc cggccgttac ctctttgatc gaggaagctc ggacgcgcgc gccacgcggt
     1801 ttgctaccgg cggcctaatt tcacgacacc ccttcgagtt tgattgctct ccgcaatagc
     1861 aggattgcgt ctgcctgctt gatgcaaagt cgtgcgcatt ttctgcttaa cgcgcgcaat
     1921 cggtaacagc gcataagaaa acaatcggcc ttaggggaga aatcgaccaa tggggttatt
     1981 atcattcctc gaccgcgagc acacggtcgc gaaacccgga tatagccgct ggatggtgcc
     2041 gccagctgcg ctgtgcgtgc atctatgcat cgggcaggcg tacgcctaca gcgtgttcaa
     2101 tctgccgatg acgaagctga tcggcataag ccagtctgcc ccgaatgact ggaagctcac
     2161 gcaactcggc tggattttct cgatcgctat cttcttcctt ggcgtttcat cggccgtttt
     2221 cggccggtgg gtcgaggaag gcggcccgcg ccgcgcaatg ttcaccgccg ctctctgctg
     2281 ggccggcggc tttttcatct cggcgttcgg cgtctacgcg cacacgctct ggatcattta
     2341 tctcggatat ggcgtgctcg gcggcatcgc gctgggcatt ggctacatct cgccggtttc
     2401 gacactgatc aaatggtttc ccgaccgacc cggaatggcc accggcatgg ccatcatggg
     2461 atttggcggt ggtgctttca tcgcatctcc gctttcagta tggctaatgg gcaaattcac
     2521 gactgccacg catatcggcg tcgctgaaac cttcatcgtc ctcgg
//
LOCUS       DASSRL010000129         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_56000, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000129 DASSRL010000000
VERSION     DASSRL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56000"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /gene="lpdA"
                     /locus_tag="VFL49_02750"
     CDS             complement(<1..1098)
                     /gene="lpdA"
                     /locus_tag="VFL49_02750"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7910923.1"
                     /translation="MVYDLIVIGTGPGGYVCAIRAAQLGLKTAVVEKRATFGGTCLNI
                     GCIPSKALLHASELFEEAGHTFSKMGIGVSSPKLDLKGMMAFKDQGVDGNVKGVEFLL
                     KKNKVDAFHGAGRIIAPGKVEVKSADNRTQTLEAKSIVIATGSDVARLKGIEIDEKRI
                     VSSTGALSLPEVPQHLLVVGAGVIGLELGSVWRRLGAQVTVVEFLDGVLPGMDGEVRR
                     QAQRLLEKQGMTFKLSSKVTAVDASGKRLKATVEPAKGGVAETIEADIVLVAIGRVPY
                     TDGLGLGDVGVKLDERGRVIVDHYYATNVPSIWAIGDVIAGPMLAHKAEDEGVAVAEI
                     LVGQAGHVNYDAIPNVVYTYPEIAIVGKSEEE"
     gene            complement(1209..2468)
                     /gene="odhB"
                     /locus_tag="VFL49_02755"
     CDS             complement(1209..2468)
                     /gene="odhB"
                     /locus_tag="VFL49_02755"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET7910924.1"
                     /translation="MADIRVPALGESVTEATVGKWFKQPGDAVAVDEPLVELETDKVT
                     LEVPAPAAGVLADVAVKNGDTVAVGALLGQIKEGAGAQPRPAAAATATKPAASPTGAP
                     DEKTSKTKPIGAGPEQPQPREMAVPPSVRKIAAESGVDPSKVSGTGLRGQVTKGDMLH
                     AIERAAAQPTPVSAPVHMRAPSPADDAAREERVHMTRLRQTIARRLKDAQNTAAMLTT
                     FNEVDMSAVMHLRSQYKDLFEKKHGVKLGFMGFFVRACIQALKEIPAVNAEIDGTDIV
                     YKNYYHIGIAVGTDKGLVVPVVRDAQAMSLADIEKTIADFGRRAREGSLKIEEMQGGT
                     FTITNGGVYGSLMSTPILNAPQSGILGMHKIQERPVVVAGKIEARPMMYLALSYDHRI
                     VDGREAVTFLVRVKEGLEDPARVVLDL"
     gene            complement(2504..>4327)
                     /locus_tag="VFL49_02760"
     CDS             complement(2504..>4327)
                     /locus_tag="VFL49_02760"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7910925.1"
                     /translation="TPDERTMVMPLLIHGDAAFAGQGVVAECFGLSGLKGYRTGGSLH
                     FIVNNQIGFTTYPRYSRSSPYPSDVAKMIEALIFHVNGDDPEAVVFAAKVATEYRQKF
                     QKPVVIDMFCYRRFGHNEGDEPSFTQPLMYKKIRAHPTTLEIYGKKLVGEGVVTEAEV
                     GKMKADWRARLDAEFESVQGYKPNKADWLDGRWSGLKAARDADDPRRGNTGVDAGLLH
                     DIGRKITALPEGFAVHKTIQRFLDHRAKEIESGEGIDWATAEALAFCSLLKEGHPVRL
                     SGQDSERGTFSQRHSVLFDQNTEARHTPFNHLGEPQARYEVLNSMLSEEAVLGFEYGY
                     STSEPNTLTLWEAQFGDFANGAQVVFDQFISSGERKWLRMSGLVCLLPHGYEGQGPEH
                     SSARLERFLQMCAEDNMQVANCTTPANYFHILRRQLKREIRKPLILMTPKSLLRHKRA
                     VSRLDEMTNGTSFHRLLWDDAQLLPSEKIKLVADDKIRRVVMCSGKVYYDLYEEREKR
                     DIDDIYILRIEQLYPFPTKALVAELSRFKQAEMVWCQEEPRNMGAWFFVDVFLQWILN
                     QIGAKHRTMRYAGRPASASTAVGQMSKHLAQLKQFLDDALG"
BASE COUNT          800 a         1363 c         1265 g          899 t
ORIGIN      
        1 ctcctcttcg ctttttccga cgatggcgat ttccgggtaa gtgtatacga cgtttggaat
       61 ggcgtcgtag ttcacgtggc cagcctgccc gaccagaatt tccgcgactg caacaccttc
      121 gtcctcggct ttatgcgcca acatcggccc cgcgataacg tcgccgatcg cccagatgct
      181 ggggacattt gtcgcgtagt aatgatcgac aatcacgcgc ccgcgttcgt caagcttcac
      241 gccgacatcg ccgaggccaa gaccgtcggt gtagggtact cgcccgatcg cgaccagaac
      301 gatatccgcc tcgattgttt cggcaactcc gcccttcgcc ggctcgaccg tcgccttcaa
      361 ccgttttccg ctcgcgtcga cagcggtcac cttcgacgac aatttgaacg tcattccctg
      421 cttttcgagc agccgttgcg cctgacggcg gacttccccg tccatgccag gcagaacacc
      481 gtcaaggaat tccacgacgg tcacctgcgc gccaagccgc cgccagacgg agcccagctc
      541 gagcccgatc accccggccc cgacgacaag cagatgctgc gggacctccg gcagcgacag
      601 cgcgccggtc gatgagacga tgcgtttttc gtcaatctcg atgcccttca ggcgcgcgac
      661 atccgagcct gtggcaatga cgatggattt ggcttccagc gtctgcgtcc tgttgtcggc
      721 gcttttgacc tccaccttgc cgggggcgat gattcgcccg gcgccatgaa aggcatcgac
      781 cttattcttc ttcagcagga attcgacgcc cttcacattg ccgtcgacgc cctgatcctt
      841 gaaggccatc atgcccttga gatcgagctt tggcgacgaa acgccgatcc ccattttgct
      901 gaacgtgtgg cctgcttcct cgaaaagctc ggaagcgtgc aacagggcct tcgagggaat
      961 gcagccgatg ttgagacagg tcccgccaaa cgtggcgcgc ttctccacga ccgcggtctt
     1021 cagcccaagc tgcgccgcgc ggattgcaca gacatagccg ccagggccgg taccgatgac
     1081 gatgaggtcg tagaccataa atgcactccg cactcctaat tgtcatggcc gggctcgtcc
     1141 cggccatcca cgtctcctga aactaagacg tggatgcccg gcacaaggcc gggcatgacg
     1201 gaggaaactt acagatcgag cacaacccgc gccgggtctt ccaacccttc cttcacccgc
     1261 acaaggaaag tgaccgcctc gcggccgtcg acaatgcgat ggtcatatga aagcgcgaga
     1321 tacatcatcg gccgcgcttc gatcttgccg gcaacgacga caggacgttc ctgaatcttg
     1381 tgcatgccga gaatgccgga ttgaggcgcg ttgagaattg gcgtcgacat cagtgaaccg
     1441 tatacaccgc cattggtgat ggtgaacgtg ccgccctgca tctcctcaat cttgagactg
     1501 ccctcgcgcg cacggcggcc gaagtctgcg atcgttttct cgatgtcggc gagcgacatt
     1561 gcctgagcgt cgcgcacgac gggaacaacg agccctttat cagtgccgac ggcaataccg
     1621 atgtgataat agtttttgta cacaatatcg gtgccgtcga tctctgcatt caccgcagga
     1681 atctccttca gcgcctggat gcaggcgcgc acgaagaatc ccatgaagcc aagcttcacc
     1741 ccatgcttct tctcaaacag atccttgtat tgactgcgca gatgcatcac cgcgctcatg
     1801 tcgacctcgt tgaaggtcgt gagcatcgcg gccgtgttct gcgcatcctt caagcggcgt
     1861 gcaatcgtct gacgcagccg cgtcatatgc acgcgctctt cgcgggcagc atcgtctgca
     1921 ggtgacgggg cacgcatgtg caccggcgcg gagaccggcg tcggctgcgc cgcagcgcgc
     1981 tcgatcgcat gtagcatgtc gcctttggtg acttgcccgc gcagcccggt accgctgact
     2041 ttcgacggat cgacgccgct ctcggcggcg attttccgta ccgagggcgg caccgccatc
     2101 tcgcggggtt gcggctgttc cgggccggcg ccgatcggct ttgttttgct ggttttctcg
     2161 tcgggggcgc ccgtcgggct ggcggcgggc ttcgtggcgg tcgccgcggc ggccggtctt
     2221 ggctgcgcac ccgccccttc cttgatttgc ccgagcaatg cgcctacggc gacagtatcg
     2281 ccgttcttta ccgcgacgtc ggccagcacg cctgcggcag gtgcgggcac ctccagcgtc
     2341 actttgtcgg tttcaagctc aaccagcggc tcgtcgaccg cgaccgcatc gcccggctgt
     2401 ttgaaccact ttcccaccgt tgcttccgtc acggattcgc cgagcgcggg cacgcggatg
     2461 tcagccatgt ttgtcctctt ccgtcatggc gaagggcgaa cgatcagcca agtgcgtcgt
     2521 cgagaaactg cttgagctgc gccagatgct tcgacatttg cccgaccgcg gtcgaggccg
     2581 acgccgggcg cccggcataa cgcatggtac gatgcttggc accgatctgg ttgagtatcc
     2641 actgcaggaa aacgtcgacg aagaaccaag cccccatatt gcgcggctct tcctggcacc
     2701 agaccatctc ggcttgcttg aagcgggaaa gctccgcgac cagagccttt gtcgggaatg
     2761 gataaagctg ctcgatgcgc aagatgtaaa tgtcgtcgat gtcgcgcttc tcacgttcct
     2821 cgtaaaggtc gtagtagacc ttgccggagc acatgacgac gcggcggatc ttgtcgtcgg
     2881 cgacgagctt gattttttcg ctcggcagca gttgcgcgtc atcccacagg agcctgtgga
     2941 aagacgtccc gttggtcatt tcgtcgagac gcgacactgc gcgcttgtgg cggagcagcg
     3001 acttcggcgt catcaggatc agcggcttgc ggatctcgcg cttcaactga cggcgcagaa
     3061 tatggaagta atttgcaggt gttgtgcagt tggcaacctg catgttgtct tccgcgcaca
     3121 tctgcagaaa gcgctcgagc cgcgccgacg aatgctccgg cccctgcccc tcgtaaccgt
     3181 gcggcagcag gcagacgagt cccgacatgc gcagccattt gcgctcgccc gatgagataa
     3241 actggtcaaa cacgacctgc gcgccgttcg cgaaatcgcc gaactgcgcc tcccacaagg
     3301 tgagcgtatt cggctcagag gtcgaatagc cgtattcaaa gccgaggacg gcctcttccg
     3361 acagcatcga gttgaggacc tcgtagcgcg cttggggctc gccgagatga ttgaaaggag
     3421 tatgacgtgc ctcggtattc tgatcgaaca gcaccgagtg ccgttgtgag aaagtacctc
     3481 gttcactgtc ctgcccggac aggcgaacag ggtgcccttc cttcagcaaa gagcagaatg
     3541 cgagtgcttc ggctgtcgcc cagtcgattc cttcgccgct ttcgatttcc ttggcgcgat
     3601 ggtcgaggaa gcgctggatg gttttgtgga cggcaaatcc ttccggaagg gctgtgattt
     3661 tccggccgat gtcgtgcaac agaccggcat cgacgccggt atttccccgc cgcgggtcgt
     3721 cagcatcgcg cgcggctttc agtccggacc agcgcccgtc aagccagtcg gccttgttgg
     3781 gcttgtaacc ctggacagac tcgaattcgg cgtcgagccg agcgcgccag tcggccttca
     3841 tcttgccgac ttctgcttcg gtgacgaccc cctcgcccac cagtttcttg ccgtagatct
     3901 caagcgtggt cggatgcgcg cggattttct tgtacatcag cggctgggtg aacgaaggct
     3961 cgtcgccctc gttgtggccg aagcggcggt agcagaacat atcgatgacg acgggcttct
     4021 ggaatttctg ccggtactcg gtagcgactt tcgccgcgaa cacgaccgct tcgggatcgt
     4081 cgccgtttac gtggaaaata agcgcctcga tcatcttggc gacatctgat ggataaggcg
     4141 acgagcgcga gtaacgcggg taggtcgtaa atccgatctg gttgttgacg atgaaatgca
     4201 gcgagccgcc ggtacggtag cctttaaggc ccgacaaacc gaagcattcc gccaccacgc
     4261 cctggccggc gaacgcggca tcgccatgga tcagcagcgg catcaccatc gtacgctcat
     4321 ctggagt
//
LOCUS       DASSRL010000130         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_738630, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000130 DASSRL010000000
VERSION     DASSRL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738630"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(564..>2840)
                     /locus_tag="VFL49_02765"
     CDS             complement(564..>2840)
                     /locus_tag="VFL49_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HET7910926.1"
                     /translation="EAEAELGSAEKIFTDSTTALADLTAQRNRFDNAAREQSERIAKI
                     SSEITAIERELSTLHSADNAPLAAAVEGAQAALTEAEAAAVAAEAAHNVARGDLESAR
                     EALSESEKRVQRLETEAKTLSKMLSLETRSLWPPVIDHVSVEKGYEKALGAALGDDLD
                     APIDPSSPMHWSEIGGDGADPVLPDGVEPLARYVKAPAQLGRRLAQVGVVERDAGARL
                     SRGIKTGQRLVSREGDLWRWDGFVAAAHAPTGAARRLAQRSRFTEIDAELESARGDVE
                     AKRHAAEAAQSAFDAANSTETEMRARERDLNRQAARLSALAEARARLTMSRDEAAATK
                     AEAESSLSALAPAADMETKLAQVHDELANKRAKLAEVQAEQQAIVREAELADRRLSHL
                     FSDRNGWVERQQGARTQIATLEQRIAETKRDRTELENAPQVFAEKRSALITEVHSAET
                     ERRACADRLQQAENALAEADRDARAALEAASAAREDLARAEERFEGAKRRLTDVGREI
                     HDMLEIPPSAVAELAEIKPTDPLPDLAEIESTLERLRRERERLGAVNLRAEEELREVE
                     AQHASLTTERDDLVEAIKKLRLGIQSLNREARERLLASFEQVNKHFQHLFTQLFGGGT
                     AELQLVESEDPLEAGLEILAKPPGKKPATLSLLSGGEQALTALALIFAVFLTNPAPIC
                     VLDEVDAPLDDHNVERFCDLLDEMIKSTDTRFVIITHNPITMARMNRLYGVTMAERGV
                     SQLVSVDLEAAVRFREAS"
BASE COUNT          465 a          889 c          903 g          583 t
ORIGIN      
        1 aacagattat ctcaggtgac atcgcaacag ccaacggcac cccggtaggg gcgcgcggcg
       61 gcatcctggt actttggtgg aagacattga agctttggct tttaccggac ggcaggcaaa
      121 aaaagacccc gagcgcaaac ccggggtctc ttcgccagac ggctattcgg ggggtaaggg
      181 gggagccgcc cagctgaaat acacgcggcg ttaacgcagg ggtgggggat tggttcccgg
      241 cggtattgcg atgacttaga atttatccac aggatcaaaa tcggtcgtca gatggtcaag
      301 gtcctcaagt tccatggctt tcggatgcaa attcggaggg tgtcacttat gagcttggtg
      361 atgatcgaat gtccggtgac cgggagagct gcttcgactg caatcgaaac cgaggcgcgc
      421 tcgttccgga gattgccgaa ggttaccgca catatgcgct gtccggtctg cggccgggat
      481 cacgaatgga cagtcagttc cgcctggctt tccggcgaac cacgtctcgt agcgcaaagt
      541 cagatccaga agacagctgc ggcttagctg gcttcgcgga agcgcaccgc ggcttcgaga
      601 tcgaccgaaa cgagttgcga cacgccgcgt tcggccatgg tgacgccgta gaggcggttc
      661 atgcgcgcca tcgtaatcgg gttgtgtgtg atgatcacga aacgcgtgtc ggtcgacttg
      721 atcatttcgt cgagcagatc gcagaagcgt tcgacgttgt gatcgtcgag cggggcgtcc
      781 acttcgtcca gcacgcagat cggcgccggg ttggtcagga acacagcgaa tatcagtgcg
      841 agcgcggtca gcgcttgttc gccgcccgac agaagcgaca acgtcgccgg cttctttccc
      901 ggcggttttg ccagaatttc caaaccggcc tcgagcgggt cttcgctctc gaccagctgc
      961 agctcggcgg tgccgccgcc gaacagctgg gtgaacagat gctggaaatg cttgttgacc
     1021 tgttcgaacg atgccagcag ccgctcgcgg gcctctcggt tgaggctctg gatgccgagc
     1081 cgaagcttct tgattgcttc gaccaggtcg tcgcgctcgg tcgtcagtga tgcatgctgg
     1141 gcctcgacct cgcgcagctc ttcttcggcg cgaagattga ctgcaccgag ccgttcccgc
     1201 tcgcgccgca gccgctcgag ggtggattct atctcggcaa gatccggcaa cgggtcggtc
     1261 ggcttgatct cggcaagctc cgcaaccgcc gacggcggaa tttccagcat gtcgtgaatt
     1321 tcacggccga catcggtaag tcttcgcttg gcaccctcga agcgctcctc agcgcgggca
     1381 agatcttcgc gtgcagcgct tgctgcctcg agggccgcgc gcgcatcgcg gtccgcttcc
     1441 gccagcgcgt tctcggcctg ttgcaggcga tcggcgcagg cgcgacgttc ggtttccgcg
     1501 gaatgcactt cggtaatcag ggcgctgcgt ttttccgcga acacctgcgg cgcgttttcc
     1561 agctctgtgc gatcacgttt ggtctcagca atacgctgct ctagcgtcgc gatctgcgtg
     1621 cgagcgcctt gctgacgttc aacccagcca ttcctgtcac tgaaaaggtg ggacaaccgc
     1681 cggtcggcga gttcggcctc acgcacgatc gcctgctgct cggcctgcac ttcggcaagc
     1741 ttggcgcgct tatttgcaag ctcgtcgtgc acctgcgcaa gcttcgtttc catgtccgca
     1801 gcgggcgcaa gcgcgctgag cgaggattcg gcttcagcct ttgtcgcggc agcctcgtca
     1861 cggctcatgg tcagccgtgc ccgcgcttcg gccagtgctg aaagccgcgc agcctgccgg
     1921 ttgaggtcgc gctcgcgggc ccgcatttcc gtttcagtgc tgtttgctgc atcaaaggcc
     1981 gactgcgctg cctccgccgc gtggcgcttg gcttcgacgt cgccacgcgc gctttcgagc
     2041 tctgcgtcga tctccgtgaa ccggcttcgt tgtgccaggc gccgtgcggc accggtaggg
     2101 gcgtgcgcgg ctgcgacgaa accatcccag cgccacaggt cgccctcgcg cgataccaat
     2161 cgctgtccgg tcttgatgcc cctggaaaga cgcgcgcctg catcgcgctc gactacgccg
     2221 acctgcgcga gacgccgccc gagttgcgcc ggcgccttta cgtagcgggc gagcggctcg
     2281 accccgtcgg gcagtacggg atcggcaccg tcgcccccga tctcgctcca atgcatggga
     2341 gacgatgggt cgatcggggc atcgaggtcg tccccgagcg cggcacccag cgccttttcg
     2401 taacctttct cgaccgaaac gtgatcgatg accggcggcc atagactgcg tgtctcaagc
     2461 gacagcatct tcgaaagcgt ctttgcctcg gtttccagcc gctgcacgcg tttttccgat
     2521 tccgacagtg cctcgcgggc cgattcaaga tcgccgcgcg cgacattgtg cgcggcttcc
     2581 gcggcgaccg ccgcggcttc cgcctccgta agagctgcct gcgcgccctc gacagccgcg
     2641 gcgagcggag cgttatcggc gctgtgtagc gttgaaagtt cacgctcgat cgcggtgatc
     2701 tccgaggaga tttttgcgat gcgctcgctc tgctcgcgcg cggcattatc gaagcggttg
     2761 cgctgggcgg tgaggtcggc aagcgcagtc gtgctatctg tgaaaatctt ttcggcagag
     2821 ccgagttcgg cctcggcctc
//
LOCUS       DASSRL010000131         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_633726, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000131 DASSRL010000000
VERSION     DASSRL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633726"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..310)
                     /locus_tag="VFL49_02770"
     CDS             complement(35..310)
                     /locus_tag="VFL49_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HET7910927.1"
                     /translation="MTEPHRPRVKYVIGPDGSPLTIADLPPPTTKRWVIRRKAEVVAA
                     VRGGLLSLEEACARYTLTVEEFLSWQYSIDQHGLAGLRTTRIQQYRQ"
     gene            616..>2424
                     /locus_tag="VFL49_02775"
     CDS             616..>2424
                     /locus_tag="VFL49_02775"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET7910928.1"
                     /translation="MVAAAYFLLAKLGLQLASINPSASPIWAPTGLAFAAVVLGGVRF
                     FPAILIGAFAANAVTAGTLETSAAIAVGNTLEGVVGGYLITRWCGGAEAFVTPARIAK
                     FAIVCVGLPTMMSATIGVATLYLAGFAAEPNLAPIWITWWLGDTAGALVVTPLIVLWA
                     VTDWRAIDGRELRDMAAVFGCTVLVGLITFSPLLPRSQYTSPLGFLAIVPLLWAALRR
                     GPRDTATVALILTIFAIWATIAQVGPFARMGLNESFLLLLTFMLSMSVPSLALSADVA
                     MRRRSEDDLRRIQAELDQRVKERTAALADANQQLGSANSKLAEANTQLADANIQLSEA
                     QRLANLGSWSWDVVKNRITWSDRLHGIYGLAPSEFGGTFADFLTLIHPDDRAQVQASL
                     TRALKTGGDFSHEERIIRGDGHVRHLHSVGEVVRDQNGSAVRILGVCLDVTERKEAET
                     ALRESEQNVRMLLTGLRDYAIYMLDLDGHVRSWNAGAEQIKGYRADEIVGQHFRKFYT
                     EDDRAQNVPERALEIAIREGKYESEGWLLRKDGSRFYANELIETIRNDMGELAGFAKI
                     TRDVTAQHEAQIALDETREQLAQAQKMEALGQLTGGI"
BASE COUNT          455 a          734 c          747 g          488 t
ORIGIN      
        1 gcttcgccgg cgtgcaaacc aaattccggg agcgttactg gcgatactgc tggatgcgtg
       61 tcgtgcgcaa cccggcaagg ccgtgctgat cgatcgagta ctgccacgag aggaattctt
      121 cgacggtgag tgtgtaccgg gcgcaggcct cttccagcga cagcaggccg ccccgcaccg
      181 ctgcaaccac ctctgccttg cggcggatga cccagcgctt ggttgtcggc ggcggcagat
      241 ccgcaatggt cagcggactg ccatctggcc cgattacata tttgaccctc gggcgatggg
      301 gctcggtcat tggtatactc acaaacaaga ctcgactgac agttactgcg gaaaaactac
      361 aggcctcggc ttaaaatttg cctaagcatg gcaaatgcat caggcaatga ttgccgggtt
      421 tccgctaacc ttttggttac agatggagcg cggtgggcaa cgacggggcg gtgcggcaaa
      481 gcgccgtaat tgcagctaat atgcccacag gacgggcttc ctgggatttt gggtcgggtg
      541 aaaaagttcc ctgctgcttc ttcgtcggga caatcaaaac ggggtggtct cggcgttttt
      601 ttcgcttggc tgccggtggt ggccgccgcc tattttcttc tcgcaaagct cggtcttcag
      661 cttgcttcta taaaccccag cgccagcccg atctgggcgc cgacgggctt ggccttcgcg
      721 gccgtcgttc tgggtggcgt tcgtttcttt ccggcaatcc tgatcggcgc attcgccgcc
      781 aacgccgtaa ctgcgggcac cctggaaacg tcggctgcga ttgcggtcgg caatacgctg
      841 gaaggggtgg tgggcggtta cctgattacg cggtggtgtg gcggcgccga ggcttttgtg
      901 acccccgcgc gaatcgcaaa gttcgcaatc gtctgcgtcg gcctgccgac catgatgagc
      961 gccacgatcg gtgtcgccac gctgtatttg gccggctttg cggctgagcc taatctcgcg
     1021 ccgatctgga tcacatggtg gcttggcgat accgccggag cgctggtagt cacccctctt
     1081 atcgtcctct gggccgtaac agactggcgt gccatcgacg ggcgcgagtt gcgcgacatg
     1141 gctgcagtgt tcggctgcac cgttctggtt gggctcatca cgttcagccc tctattgccg
     1201 cgatcccaat acacgagtcc gctcggtttt cttgcaatag tgccgctgct ctgggcagct
     1261 ttgcgccgcg gaccgcgcga cactgcgacg gtcgcactca ttctgacgat tttcgccata
     1321 tgggcgacga tcgcgcaggt tggcccattc gcgcgaatgg gtctcaacga atcattcctg
     1381 cttctgctca cctttatgct cagcatgtcg gtaccaagcc tcgccctgag cgccgacgtg
     1441 gcaatgcgcc gccgcagcga ggacgatctc cggcgcatcc aggccgagct tgaccagcgc
     1501 gtgaaggagc gtactgccgc gctcgcggac gccaatcagc agctcggcag cgcgaacagc
     1561 aaactggccg aggccaatac ccagctcgcc gatgccaaca tccagctctc cgaagcacag
     1621 cgcctcgcca atcttgggag ctggtcgtgg gatgttgtga aaaatcgcat cacctggtcg
     1681 gatcgccttc atgggatcta cgggttggcg ccgagtgaat ttggcggcac ctttgcggac
     1741 ttcctgaccc tcatccatcc ggatgatcgt gcccaggtgc aggcgagctt gacccgcgcc
     1801 ctgaaaacag gcggtgattt cagccacgaa gagcggatca tccgcggcga cgggcatgtc
     1861 cgtcatttac acagcgtcgg ggaagtcgtc cgcgaccaga atggcagcgc tgtccgcatc
     1921 ctcggcgtct gtctcgatgt gacggagcgc aaggaagccg aaacagctct ccgcgagtca
     1981 gagcagaacg tgcggatgct tctgacgggc cttcgcgact atgccatcta catgctcgat
     2041 ctcgacggcc atgtgcgcag ctggaatgcc ggtgccgagc agatcaaggg ctaccgcgcc
     2101 gatgagatcg tcggccagca cttccgcaaa ttttacacgg aagacgaccg ggcgcagaac
     2161 gtgccggagc gcgcactgga gatcgcaata cgcgaaggca aatacgagtc ggaaggctgg
     2221 ctcctgcgca aggacggcag ccgtttctat gccaacgagt tgatcgagac cattcgtaac
     2281 gacatgggcg aactggcggg cttcgccaag ataacccgtg acgtcaccgc gcagcatgag
     2341 gcgcagatcg cgctcgatga gacccgtgag caactggccc aagcacagaa gatggaagct
     2401 ctcggccagc tcaccggcgg catc
//
LOCUS       DASSRL010000132         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1001120, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000132 DASSRL010000000
VERSION     DASSRL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001120"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1984
                     /locus_tag="VFL49_02780"
     CDS             221..1984
                     /locus_tag="VFL49_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7910929.1"
                     /translation="MDELCKYANRPFAFIGRYLRRRRFAHLAILIAVVGAVSCSVATQ
                     YGVKFLIDTLAQGPKAANIVAPFAVLAILISADNLLWRLACWIGSFTFVAVSGDLRRE
                     LFRHLTGHAPNYFLSRLPGTLTSRITATSNAVFTIENMFVCNVLPPCIATLGAIIVVL
                     TVSPAMAGVLVVIAAILIFALFHLADAGKPLHHNFADKAAAVDGEMVDVIANMPLVRA
                     FCGLRREHSRFDETVGHEMKARQRSLFYLERLRLLHAVISILLTIGLLAWAIFLWQGG
                     AATTGDVVLICTLGIAILHATRDLAVALVDVTQHMARLAEALATLLIPHELRDHPEAE
                     ALVKRGADIVFDNVSFNYPDGREVFSGLDLHIESGQRVGLVGPSGSGKSTLFGLMQRF
                     YDVQGGRILIHGQDISRVTQESVRGSISVVPQDISLLHRSVMENIRYGRPDASDAEVL
                     EAAAAARCDEFIATLPEGLATIVGDRGVKLSGGQRQRIAIGRAFLKDASILLLDEATS
                     ALDVESEEMIRDALERLMRGRTVVAIAHSLPTLRSFDRIVMLQDGRVVEDGDPDKLMQ
                     HDGPYRRLVMREMGRLTRKAA"
     gene            2232..2417
                     /locus_tag="VFL49_02785"
     CDS             2232..2417
                     /locus_tag="VFL49_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910930.1"
                     /translation="MDHKDAHSQTDGPDLVPLKAVVAFLWLVAGLVAFVALDRPGIDD
                     INKVVAAPESLAAAAVP"
     gene            2535..>2809
                     /locus_tag="VFL49_02790"
     CDS             2535..>2809
                     /locus_tag="VFL49_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910931.1"
                     /translation="MLLPSLFALHADGLIPPQLAMLCTADGELTSDDFRSLVGATLEK
                     YLPAERRRPGTAWDFLKQLRYVQLDASEPESFGVLSKALGNGGTRNIA"
BASE COUNT          491 a          879 c          847 g          592 t
ORIGIN      
        1 cccaggacgc tcgaagcatc ccaagcaatt tgcgtaatgc tcgtcactag cccccaacat
       61 ctgccacatc acatgacagc cattgcaaaa tggtcgcaga aatccgtcga actcatcgcc
      121 ggtttgcgcg ttgccagacc agatgtttga aagagttcgc gccgggatgg gggtacagcc
      181 ttggccgtga ccagagaaat ttatggacga ttttttattg atggacgagc tgtgcaaata
      241 cgccaaccgg ccattcgcct tcattggccg ctacctgcga cgccgccggt tcgcgcatct
      301 tgcgatcctt attgccgttg tcggtgccgt cagttgctcg gtggcgacgc aatacggggt
      361 gaagttcctc atcgatacgc tggcgcaagg gcctaaggcc gccaacatcg tcgctccctt
      421 cgccgttctc gccatcctga tttccgccga taatctattg tggcggctcg cctgctggat
      481 cggcagtttc acctttgttg ccgtcagcgg cgatttgcgc cgcgagctgt ttcgccatct
      541 gaccggccac gcccccaatt atttcttgag ccggttaccg ggaaccttga cgagccggat
      601 cacggcgacc tcgaatgccg tcttcaccat cgagaatatg ttcgtgtgca acgtgctgcc
      661 gccctgcatt gcgacgcttg gcgccatcat cgtggtgctc accgtgagcc cggccatggc
      721 aggcgtgctt gtcgtgatcg ccgcgatctt aatcttcgcg ctgtttcacc ttgccgatgc
      781 cggcaagcct ttgcatcaca acttcgcgga caaggcggcc gccgttgacg gcgagatggt
      841 cgacgtcatc gccaacatgc ccctggttcg tgccttctgc ggcctccgcc gggagcacag
      901 cagattcgac gagaccgtgg gccatgagat gaaggcgagg cagcgcagcc tgttctatct
      961 cgagaggctg cggctgctcc acgccgtgat ctccatattg ctgaccatcg gcttgcttgc
     1021 atgggcaatc tttctttggc agggcggagc ggcgaccacc ggcgacgtgg tgctgatctg
     1081 cacgctcgga attgccattc tgcacgcgac tcgcgatttg gcagtcgctt tggtggacgt
     1141 gacccagcat atggcccggc tcgccgaggc tctcgcgaca cttctgatcc ctcatgagct
     1201 gcgcgaccat ccggaagccg aggcactggt caagcgaggc gccgacatcg tcttcgacaa
     1261 tgtctcgttc aattatcccg atgggcggga ggtgttcagc gggctcgact tgcatatcga
     1321 gtccggtcag cgcgtgggac tggtcggccc gtccggcagc ggcaaatcca ccctgttcgg
     1381 cctgatgcaa cgcttctacg atgtgcaagg aggccgcatc ctgatccacg gtcaggatat
     1441 ctcccgcgtg acgcaagaga gcgtgcgcgg ctcgatatcc gtcgtgcccc aggatatctc
     1501 cctgctccac cgttcggtca tggagaatat ccgttatggc cgaccggacg cctcggacgc
     1561 ggaggtgctg gaagctgcag ctgcggcgag atgcgacgag ttcatcgcga cactgcccga
     1621 aggccttgcc acgatcgtgg gcgatcgcgg cgtcaaactc tcgggcggcc agcggcaacg
     1681 cattgccatc gggcgcgcct ttctgaagga cgcatcgatc ctgctgctgg acgaagccac
     1741 ttcggcgctc gacgtcgaat cggaggagat gatccgcgac gcgcttgagc gcctgatgcg
     1801 gggacgaacg gtggtcgcca tcgcccacag tctgccgacg ttgcgcagct tcgatcgcat
     1861 tgtcatgctg caagatgggc gggtcgtgga agacggcgac cccgacaagt tgatgcagca
     1921 cgacggcccc tatcgtcgtt tggtcatgcg tgagatgggc cgcctgacac ggaaggccgc
     1981 gtagccgccg tcctgggctc cttgcgaaac ggccgaacct ttgggaactc tcggaagcct
     2041 ggcccgtttg cgaaccttag cggccgatgc ggcattttcc ctgaatgccg acagttttgg
     2101 ctgcggaagc ccggtcccag cccccctgca ggccgggctt cttggtgtga actctgcgtg
     2161 aacactctcg tcgcaggcgg gaaccgagtg gccgaatttc gagttagctc ctcagatacg
     2221 caggaaaaaa cgtggaccac aaagacgcac actcgcagac tgatgggcca gatctagtcc
     2281 ctctaaaggc cgtcgttgca ttcctttggt tggtagccgg gctcgtcgcc ttcgtcgcgc
     2341 tcgaccgccc gggcatcgac gacattaaca aagtcgtggc agcgcctgag agtctagccg
     2401 ccgccgccgt gccctagcgc tgcggtttgc cgacacgtca gcgctcatgt tacctcaccg
     2461 tacgggtttt agcgatgacg cggactgcgg acagcctttt gctttttggc gctactgggg
     2521 ctctggcgca gcgcatgttg ctgccctcgc tctttgcgct gcacgccgat ggtctgattc
     2581 cgccgcagct cgccatgctc tgcactgcag acggcgagct cacgagcgac gattttcgct
     2641 cgctggtcgg ggctaccctt gaaaaatatc tgccggccga acggcgccgg ccggggacag
     2701 catgggactt cctgaagcag ctccgttacg ttcagctcga cgcatcagag cctgagagtt
     2761 tcggcgtctt gtcgaaggcg ctcggcaacg gcggcacgcg caatatcgc
//
LOCUS       DASSRL010000133         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1298517, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000133 DASSRL010000000
VERSION     DASSRL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298517"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..930)
                     /locus_tag="VFL49_02795"
     CDS             complement(142..930)
                     /locus_tag="VFL49_02795"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7910932.1"
                     /translation="MPARSSSGRRWWGVAWRLLPLAAVFLFALVAYLAAGDKGISLES
                     LVRHRAIIDDFVSAHRVMSIVAFIGIYIVTVAFSLPGATFLTVTGGFLFGLVIGAVAA
                     EIGATVGATLIFFVARTALGEPLLRRAGPRARQLAKGFRDDAFSYLLFLRLVPVFPFF
                     LVNLVPAFAGVRLRPFVAATAIGVIPAVFVFSLAGMGLDSVIAAQKASYHQCTASGEY
                     TCRMVFDPSDVLTPQLIAALVGLGLLALVPVIVKRWRARSRAVT"
     gene            1153..1287
                     /gene="rpmH"
                     /locus_tag="VFL49_02800"
     CDS             1153..1287
                     /gene="rpmH"
                     /locus_tag="VFL49_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7910933.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMATKGGRKVVSARRGRGRKRLSA
                     "
     gene            1317..1664
                     /gene="rnpA"
                     /locus_tag="VFL49_02805"
     CDS             1317..1664
                     /gene="rnpA"
                     /locus_tag="VFL49_02805"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7910934.1"
                     /translation="MERLRQRADFLAAASGIKAPSTAFVLQARKRRDQKPARFGFTVS
                     KRVGNAVERNRVRRRLREIVRLSAATRVRTGHDYVLIGRRAALDLPFDRMTEDFEGAL
                     RRVHAGQRQTEIQ"
     gene            1661..3505
                     /gene="yidC"
                     /locus_tag="VFL49_02810"
     CDS             1661..3505
                     /gene="yidC"
                     /locus_tag="VFL49_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7910935.1"
                     /translation="MTDNKNTILAIVLSALVLLGWQYFFAAPQEKARQEQLQAQQQKQ
                     NVPTPGQAKPAQPEAQSQVPGQASTPSSAAPIDRKAALANSPRIPIATDTLQGSVALK
                     GGRIDDLALVKFRETTDPKSPPIVLLSPSGTGDPFYAEFGWTGSAGANVKLPTSETVW
                     KQVGSGGLGVGHPVTLAYDNGEGLEFRRTISVDDKYLFTIKDTVSNKSGSSVTLFPYA
                     LISRHGIPPTAGYYVLHEGLIGVLGESGLQEFTYKNIEDKKSVNFDVTNGWLGITDKY
                     WAATLLPDTNARLKARFSTSMLGTVRTFQTDYLLDPQTIAPGATGSVNGRLFAGAKEV
                     SIVGINFPFVGIGGYNKELGLNHFDLLIDWGWFYFITKPMFLVMDFFYRFFGNFGLAI
                     LLVTVLVKIIFFPLANKSYASMAKMKAVQPQMMALRERYADDKAKQQQELMELYKREK
                     INPLAGCLPIVIQIPVFFSLYKVLFVTIEMRHAPFFGWIHDLSAQDPTNIFNLFGLIP
                     FDPTLLPLIGHFLHLGIWPLIMGITMWIQMKLNPAPPDPTQAMIFNWMPLIFTFMLAS
                     FPAGLVIYWAWNNLLSVVQQSVIMRKHGAKIELFDNIKTMFVKKTPQQ"
     gene            3574..4224
                     /gene="yihA"
                     /locus_tag="VFL49_02815"
     CDS             3574..4224
                     /gene="yihA"
                     /locus_tag="VFL49_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET7910936.1"
                     /translation="MATNSYTRAEIETGRKLFAGDWQFAAAAGSPASLPAMKGVEIAF
                     AGRSNVGKSSLINALTGRKNLARISRTPGRTQELIFFNGGDNLNVVDMPGYGYAAAAK
                     TKIAAWTALIDAFLRGRSNLARVYVLVDARHGLKTTDDPALDALGQAAVSHQIILTKC
                     DEMKPADLAGRIADVEAALAKRPAAFPTVLATSSYDGTGVPELRAAIGRLLAERKS"
     gene            4286..5182
                     /gene="argB"
                     /locus_tag="VFL49_02820"
     CDS             4286..5182
                     /gene="argB"
                     /locus_tag="VFL49_02820"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET7910937.1"
                     /translation="MSKAPKIDPQTQARILSEALPHMQRYDEEIVVVKYGGHAMGQEQ
                     TARDFAKDIVLLEQTAINPVVVHGGGPQIEAMLKKLGIKSEFAAGLRITDAKTVEIVE
                     MVLAGSINKQIVGYINAAGGKAIGLCGKDGNMVQARKVSRTVVDPDSNIEKVIDLGFV
                     GEPEKVDTTMLNQVIGREMIPVLAPVATSADGGTFNVNADTFAGAIAGALKAKRLLLL
                     TDVPGVLDKSKGLIADLSVSDARKLIADGTISGGMIPKVETCIYALDQGVEGVVIMDG
                     KVPHAVLLELLTDHGVGTLMHR"
BASE COUNT         1142 a         1626 c         1574 g          925 t
ORIGIN      
        1 gcccatcttg ctggactcca cgagcaccgt cgggacccca aatgccgctg ccgccgcggc
       61 caccgacagg ccgccggggc caccgccaat cacgcaaatg tcaggtctga gcgtttcggc
      121 catgtcccgg caaccttcca atcacgtgac cgcgcgcgag cgggcgcgcc agcgtttcac
      181 gataacgggt acaagcgcga gcaagccaag acccacgagc gccgcgatca attgcggcgt
      241 aagcacgtcc gaaggatcaa agaccatccg gcaggtgtat tcgccggagg ccgtgcattg
      301 atgataagaa gccttctgcg ccgcgatcac actgtcgagg cccatgccgg cgagggagaa
      361 tacaaagacg gcaggaatca caccgatggc agttgcggct acaaacggcc ggaggcgcac
      421 gcctgcgaaa gcaggaacga gattgaccag aaaaaacggg aacaccggca ccagccgcaa
      481 aaagagaaga tagctgaacg cgtcgtcgcg aaaccctttt gcgagctgcc tggcgcgcgg
      541 gcccgcccgg cgcaacaacg gctcacccag ggctgtacgc gctacaaaga agatgagcgt
      601 ggcgccaact gtcgcgccga tttcagcagc aactgcaccg atgaccagtc caaacagaaa
      661 cccgcccgtg acggtgagga aggtcgcccc cggaagcgag aaggcaactg tcacgatata
      721 aatgccgatg aaagcaacga tggacatcac gcgatgcgcg ctgacgaagt cgtcaatgat
      781 cgcgcgatga cggacgagcg actcgagcga aatcccttta tcgcctgcgg ccagatacgc
      841 gaccaaggcg aatagaaaga cggccgccaa tggcaaaagc cgccaggcga caccccacca
      901 acgtcggccc gaggatgagc gtgcaggcat tcacgctata tacacgagcc gcgctgccga
      961 acggggcgct ctcaccgcct tgtgaggctt tttgaccctc cctgagccgc cggcaccgcg
     1021 acattgacgg gcctgaggcc ctcgcttata agccgcgcaa aatcgcatgc cgacccgaac
     1081 gcgtgggttt gacggcgtat cgagggttaa cccctccttt ccccacggcg cagccagcgg
     1141 agaccatcga acgtgaagcg gacttatcaa ccgagcaagc tcgtacgtaa acgccgccac
     1201 ggtttccgcg cacgtatggc gaccaaggga gggcgcaagg tcgtgtcggc gcgccggggg
     1261 cgcggccgca agcggctgtc agcctgaccg cggcgataaa gccgcgcatt gcttccatgg
     1321 aacgattgag gcaacgggcg gacttcctgg ctgcagcgtc cgggatcaag gcgccgtcaa
     1381 cggcgttcgt gctgcaggca cgcaagcgcc gcgaccaaaa accggcccgg ttcggcttta
     1441 cagtgtcgaa aagggtcgga aatgccgttg agcgcaacag ggtccgccgc cgcctccgtg
     1501 aaatcgtgcg gctgtcggcc gcaacccggg tgcgcacggg tcatgattat gtgctgatcg
     1561 gcaggcgggc ggcgctcgat ctgccgttcg atcgtatgac agaggacttc gaaggcgcgt
     1621 tgcggcgagt acacgcaggg cagcggcaaa cggaaattca atgaccgata acaagaacac
     1681 cattcttgcc atcgtgctgt ccgcgctggt gctgcttggc tggcaatatt tcttcgccgc
     1741 tccacaggaa aaggcacggc aggaacagtt gcaggcgcag cagcagaaac agaatgtgcc
     1801 gacgcccggc caagccaaac cggcgcagcc ggaagcgcag tcgcaagtgc ccggccaggc
     1861 aagcacacct tcatccgcgg cacccatcga tcgcaaggcg gcgctggcga attcgccacg
     1921 catacccatt gccaccgaca ctttgcaggg ctcggttgct ttgaaaggcg gccgcatcga
     1981 cgacctggct ctggtcaaat tccgcgaaac gactgacccg aaatcgccgc cgatcgtttt
     2041 gctgtcccct tcgggtaccg gcgatccgtt ctatgccgag ttcggctgga cgggctccgc
     2101 cggagcgaac gtcaagctgc cgacctccga aaccgtttgg aaacaggtgg gctccggagg
     2161 actcggcgtc ggccaccccg tgactttggc ctatgacaac ggcgaaggac tcgaatttcg
     2221 ccgcaccatt tcggtcgacg acaagtatct gttcacgatc aaggataccg tcagcaacaa
     2281 gagcggaagc tcggtcacac tttttccata cgccttgatc tcgcgtcacg gaataccgcc
     2341 gaccgccgga tactacgttc tgcatgaagg tctgatcgga gtgctcggcg agtccggctt
     2401 gcaggagttc acctacaaga acatcgagga caaaaagtcc gtcaatttcg acgtcaccaa
     2461 cggctggctc ggcatcaccg acaaatattg ggctgccacc cttttgccgg acacaaacgc
     2521 gcgcctgaaa gcgcggttct cgaccagcat gctcggcacg gtcaggacat ttcagaccga
     2581 ttatctgctc gatccgcaga cgatcgcgcc gggtgcgacc ggttccgtca atggacggtt
     2641 gtttgccggc gccaaggaag tgtcgatcgt cggcatcaac tttccattcg tcggcatcgg
     2701 tggctacaac aaggagctcg gcctcaacca cttcgatctg ctgatcgatt ggggttggtt
     2761 ctatttcatc acaaagccga tgttcctcgt gatggatttc ttttaccgct tcttcggcaa
     2821 tttcggtctg gcgatcctgc tggtcaccgt gttggtcaag attattttct tcccgctggc
     2881 gaacaaatct tacgcttcga tggcgaagat gaaggccgtg cagccgcaaa tgatggctct
     2941 gcgcgagcgt tacgccgacg ataaggccaa gcagcagcag gaattgatgg agttgtacaa
     3001 gcgggaaaag atcaaccctc tggccggctg cctgccgatc gtgatccaga ttccggtctt
     3061 tttctcactc tataaggtcc tgttcgtcac gatcgagatg cgccatgcgc cattctttgg
     3121 ctggatccac gatctttcgg cgcaggatcc aacgaacatc ttcaatctgt tcgggctcat
     3181 cccgtttgac ccgacgctgc tgccgctgat cggtcatttc ctgcatctgg gcatctggcc
     3241 cctcatcatg ggcatcacga tgtggattca aatgaagctc aatccagcgc cgcccgatcc
     3301 gacgcaggcc atgatattca actggatgcc gctgatattc acgttcatgc tggcaagctt
     3361 ccctgccggc ctcgtgatct actgggcgtg gaacaatctg ctttcggtcg tgcagcagag
     3421 cgtgatcatg cgcaagcatg gggcgaagat agagctgttc gacaatatca agactatgtt
     3481 cgtaaagaag acgccacagc aataaaggct ggcaaacttg gtttcgcgtc atggccgggc
     3541 atgacgccgg acttgccggt atgttgtgtc ccaatggcaa ccaacagtta cacccgcgcg
     3601 gaaatcgaga ccggtcgcaa gctttttgcc ggcgactggc aatttgctgc cgccgcgggc
     3661 tcacccgcct cgctgccggc catgaagggc gtcgagatcg ccttcgcggg ccgttcgaat
     3721 gtcggcaagt ccagcctgat caatgcgctt acgggccgaa aaaacctcgc gcgcatctcg
     3781 cgcacgcccg gccgcacaca ggagctcata ttcttcaatg gcggtgacaa tctcaacgtc
     3841 gtggacatgc cgggttacgg ctatgccgcc gcggcgaaga caaagatcgc ggcctggacg
     3901 gcattgatcg acgcttttct gcgcgggcgg agcaacctcg cccgggttta cgtactggtc
     3961 gatgcgcggc atgggctcaa aacaacggat gatccggcgc tcgacgccct cggccaggcg
     4021 gcggtcagcc accagatcat tttgaccaag tgcgacgaga tgaagcctgc cgatctggcc
     4081 ggccgcatcg ccgacgtcga agccgccctg gccaagcggc cggcggcttt cccgacggtt
     4141 ctggcgacct cctcatacga cggaacgggc gtccccgagc tgcgcgcggc catcgggcgg
     4201 ctgctcgcgg aaagaaaaag ctagtgcatt gtcgaggcac ggccgcatcg gctagaaccg
     4261 ccggcaaccg caaaaggccg gagcgatgag caaagccccc aagatcgacc cgcagacgca
     4321 ggcccgcatc ctgtccgaag cgctgccgca catgcagcgt tacgacgagg agatcgtcgt
     4381 ggtgaaatac ggcgggcacg ccatgggtca ggaacagacg gcgcgcgact ttgcaaaaga
     4441 catcgtcctg ctcgagcaga cggcgatcaa tcccgttgtc gtgcatggcg gcggaccgca
     4501 gatcgaggcc atgctcaaga agctcggcat caagtccgag ttcgcggccg gcctgcgcat
     4561 caccgacgcg aagacggtcg agatcgtcga aatggtgctg gccggctcga tcaacaagca
     4621 gatcgtcggc tacatcaacg ccgccggcgg caaggccatc gggctgtgcg gcaaggatgg
     4681 caacatggtg caggcacgca aagtgagccg cacggtggtc gatccggatt ccaacatcga
     4741 aaaggtcatc gacctcggct tcgtgggcga acccgagaag gtcgacacca ccatgctcaa
     4801 ccaggtgatc gggcgcgaga tgatccccgt gctggcgccg gtagcgactt cggctgacgg
     4861 cggaacattc aacgtcaacg ccgatacctt cgctggagcg atcgccggcg cattgaaagc
     4921 caagcgcctg ttgctgctca ccgacgtgcc cggcgtcctc gacaagtcga agggcctgat
     4981 cgcggacctg tcggtttccg acgcacgcaa gctgatcgcc gacggcacga tttccggagg
     5041 catgatcccg aaggtcgaaa cctgcatcta cgcgctggat cagggtgtcg agggtgtcgt
     5101 cattatggac ggcaaggtcc cgcacgccgt gttgctcgaa ctgctgaccg atcacggtgt
     5161 cggcacactg atgcacaggt aaaaagaatc cactcattcc cgcgtaagcg ggaatccaga
     5221 gctcaagatt cagcattggc aaattgccct gggtccccgc tttcgcg
//
LOCUS       DASSRL010000134         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_563590, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000134 DASSRL010000000
VERSION     DASSRL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563590"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFL49_02825"
     CDS             <1..303
                     /locus_tag="VFL49_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910938.1"
                     /translation="EALHTIDRDGLETLENSPQAISMVLGPSGEIISDTLSETEGLLY
                     QDVDISQCVEPKQFHDVVGYYNRFDVFDLKVNRKRLAPIAFVDGFPGVTDDEMMPG"
     gene            complement(409..1413)
                     /locus_tag="VFL49_02830"
     CDS             complement(409..1413)
                     /locus_tag="VFL49_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7910939.1"
                     /translation="MKIIFAILLLVVALLAGPQNGAAVAQPAAGAAADVKMPAIPKGK
                     ETDVKFYAANGNAVTGREALDQMPKAALVLWLAGNQFFAMDDVVHAFQKDHPGTTVAL
                     ITLPPGLLLQAIKAGGWVYGDTVYRGRPDVYASVNLGHLKQLKTSGTMESYMVYMHNE
                     LQIMVAKNNPKNITGIKDLVREDVRTSMPNPVNEGIMQFYTRKVLERHGLWQAISGGK
                     ECVSCQTTKNNWFTAVHHRETPDRIRAGQADAGVVWKTEVLEARRAGADVDAIGLPDE
                     DSLRSEVAYVIGTLSASPHRKEADAFLGFLRTPAGQDAYAKFGFVKATEAELKLRPIN
                     "
     gene            complement(1492..1788)
                     /locus_tag="VFL49_02835"
     CDS             complement(1492..1788)
                     /locus_tag="VFL49_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910940.1"
                     /translation="MASPEDRPGPNQIPERDTMSWDETLPVSIVALRAEGYSPDGDNI
                     IISLRTKFSSAERKYSVPVQCFFDLIVDLQRINASPLKEIEPMLPLEEPVIAAE"
     gene            complement(1905..>2492)
                     /locus_tag="VFL49_02840"
     CDS             complement(1905..>2492)
                     /locus_tag="VFL49_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HET7910941.1"
                     /translation="AAELQQPHMTWRASDVAMGEIDGEPRDLVTLAYVLNELAPEARQ
                     AVLERLWRQTADTLIIVEPGTPAGWQRILAARRQLIEAGAYVIAPCSHANECPLQPPD
                     WCHFARRVARSRLHRQAKGADVPWEDEKFSYVAVSRKPTAATGARVIARPRKASGRVT
                     LKLCRPDGSAGEQPFSRRDGAPFKHAWRSDWGSSL"
BASE COUNT          492 a          764 c          718 g          518 t
ORIGIN      
        1 gaggccctgc acacaatcga tcgcgacggc ctcgagaccc ttgagaacag cccccaggcc
       61 atatcgatgg tgcttggccc ctcaggcgag atcatcagcg atacactgtc ggagacggaa
      121 ggtctcctct atcaggacgt cgacatttca caatgcgtcg agcccaaaca gtttcacgat
      181 gtggtgggct actacaatcg ctttgacgtg ttcgatctca aagtcaatcg caagcgtctc
      241 gcgccaattg cgtttgttga cgggttcccc ggtgttactg acgatgagat gatgcccggg
      301 taaagcggcc acggctatgc ggccgggtgc caatatccag tcaacgtcaa tgacgaagtt
      361 cgcgtctaag aacacgccta aagatttcaa ctatcgaagc aggttggctc aattgatcgg
      421 cctaagctta agctctgcct cggtcgcttt cacaaagcca aatttggcgt aagcgtcctg
      481 gccggccggt gtccgcaaaa agccaagaaa ggcgtcggct tccttgcgat gcggagatgc
      541 gctcagagtg ccgatcacat aggcgacttc gctgcgcaaa ctgtcttcgt ccggcagtcc
      601 aatcgcgtcg acatcggcgc cggcgcgacg ggcctccagc acctccgtct tccagacgac
      661 tccggcatcg gcttggccgg ccctgatccg gtccggggtt tcgcgatggt gcacggccgt
      721 gaaccagttg ttcttcgtcg tctgacagga cacgcattct ttgccgccgg agatcgcctg
      781 ccagagtcca tgacgctcga gcaccttgcg ggtgtagaac tgcatgatcc cttcattgac
      841 aggattcggc atcgacgtgc gcacgtcttc gcgtaccaga tccttgatgc cggtgatatt
      901 cttcgggttg tttttcgcca ccatgatctg cagttcgttg tgcatgtaga ccatgtagct
      961 ttccatggtg cccgaggtct tgagctgctt gaggtgaccc aaattcaccg acgcatagac
     1021 gtccggtcgc ccccgataga ccgtgtcgcc atacacccag cccccggcct tgatggcctg
     1081 cagcagcaaa cccggaggaa gggtgataag cgcaaccgtc gtgcccgggt gatccttctg
     1141 gaatgcgtga acgacatcgt ccatggcaaa aaactgattg cccgcgagcc aaaggacgag
     1201 ggcggccttg ggcatctggt ccaaagcctc acggccggtg accgcattgc cattcgccgc
     1261 atagaatttg acgtccgttt ccttgccttt cggaatggcg ggcattttga cgtccgctgc
     1321 ggcaccggca gcgggttggg caacagccgc gccgttttgc gggcccgcga gcagagccac
     1381 gaccaacaac aaaattgcaa aaattatttt catcgcccac ccccggaatc gaatgcctgc
     1441 gtcgaagggt gcgatgttcc ggatacgcca gtcaaggaca gtggccggtt cttattcggc
     1501 ggctatcacc ggctcttcca gcggaagcat cggctcgatc tctttcagag gcgatgcatt
     1561 gattcgctgc aggtcgacga tcagatcgaa aaagcattga accggcactg aatattttcg
     1621 ctcggcgctg gagaatttcg tgcgcaatga aatgatgatg ttgtcgccgt cgggcgagta
     1681 gccttcggct cttaacgcga cgatgctgac cggcaatgtt tcatcccagc tcatggtgtc
     1741 acgttcaggt atctggttgg ggcccgggcg gtcttcggga cttgccactt ttcaactgtt
     1801 gaacgctgac cctcggcaag agttccgttt gatccggcac tcgtcgaccg gaaccaggca
     1861 gtatttagaa gaagtttatc atctgtgccc tcgcggacat cgcgtcacaa cgaagagccc
     1921 caatcgctgc gccacgcatg cttgaacggt gcgccgtcgc ggcgcgagaa cggttgctcg
     1981 cccgccgatc cgtcgggacg gcacagcttc agcgtgacgc ggccgctggc ctttcgagga
     2041 cgcgcaatga cacgtgcccc ggtcgccgct gtcggcttcc gcgagaccgc cacgtagctg
     2101 aacttctcgt cttcccaggg cacgtcggca cccttggcct gccggtgcag gcgtgagcgc
     2161 gcaacgcgcc gggcaaaatg gcaccagtcc ggcggctgca gcggacattc gttcgcatgc
     2221 gagcatggcg cgatcacata agcgccggcc tcgatcagct gtcgccgcgc ggcgaggata
     2281 cgctgccagc cggccggcgt acccggttca acgatgatga gcgtatccgc ggtctgccgc
     2341 cacaaccgtt ccaaaacagc ttgccgggcc tccggtgcaa gctcgttcaa tacataagcc
     2401 aacgtgacca gatcgcgcgg ttcgccatcg atttcgccca tagcaacatc agaagcgcgc
     2461 cacgtcatgt gcggctgctg cagctccgcc gc
//
LOCUS       DASSRL010000135         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_3902, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000135 DASSRL010000000
VERSION     DASSRL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFL49_02845"
     CDS             complement(<1..210)
                     /locus_tag="VFL49_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910942.1"
                     /translation="MERVVDPLVKKPSVLSSRSETIGFVVMVAALVIAPFVTYPLFLM
                     QVMCFALFACAFNLLIGYVGLLSFGH"
     gene            complement(210..1097)
                     /locus_tag="VFL49_02850"
     CDS             complement(210..1097)
                     /locus_tag="VFL49_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7910943.1"
                     /translation="MFEIFGIPSTALFGQLLIGLINGSFYALLSLGLAVIFGMLNIIN
                     FSHGAQYMMGAFTAYLILQYSGLGYWPALIIAPVLVGITGILVERFFLQWLYKLDHLY
                     GLLLTFGLALIFEGVARNYFGSAGLPYTVPDSLRGGQNLGFMFLPNYRAWVVAASLVV
                     CFGTWFVIERTRLGAYLRAATENPTLVRAFGINVSRMITLTYGFGVALAAFAGVMAAP
                     IYNVSPQMGSELIIVVFAVVVIGGMGSILGAIVTGFGLGVIEGLTKVFFPEASNTVVF
                     VIMAIVLLVRPSGLFGRRA"
     gene            complement(1161..2369)
                     /locus_tag="VFL49_02855"
     CDS             complement(1161..2369)
                     /locus_tag="VFL49_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7910944.1"
                     /translation="MRKTLGLAALAAVLACGGANAQQVNVKIGVLSDMSSLYADIGGP
                     GSVAAAKMAIADFTKDHPNVKVELISGDHQNKPDIGAQIANQWYDVDKVDLIVDVPNS
                     GVALAVSQITANKNKVFIVSGAAASDITGPKCNANTIHWTYDTWMLANGTGTAIVKTG
                     GDTWFFLTSDYAFGHALERDTAAAVEKAGGKVLGKVRHPLNTNDFSSFLLQAQSSKAK
                     VIGLANAGGDTINSIKQAAEFGIVKGGQSLAGLLVFASDVAALGLPTAQGLVLTETWY
                     WDMNDANRAWTKRWQQERQGKWPTMIHAGVYSGITHYLKAAVALKGNVSDGKAVVAQM
                     KSTPTDDPLFGKGTVRPDGRKVHPAYLLEVKKPSESKHPGDFYKVRATIPADQAFRPL
                     KEGNCPLVSG"
BASE COUNT          532 a          753 c          719 g          440 t
ORIGIN      
        1 atggccaaac gataagagcc cgacatatcc gatcagcaga ttgaacgcac aggcaaacag
       61 cgcgaaacac atcacctgca tcaggaacag cggataggtc acgaacggcg caatcaccag
      121 cgccgccacc atgaccacaa agccgatcgt ctcgcttcgc gagctcagca cggacggctt
      181 tttcacgagc gggtcgacga cgcgttccat caggctctcc gcccgaacag gccggaggga
      241 cgcaccagca acacaatcgc catgatgacg aaaacgacgg tgttcgatgc ttccggaaaa
      301 aagaccttgg tcaggccctc gatcacgccg aggccgaagc cggtaacgat cgcgcccaga
      361 atcgatccca tgccgccgat aacgaccacc gcaaagacga cgatgatcag ctccgagccc
      421 atctgcggcg aaacattgta gatcggggcg gccatcacgc cggcgaatgc ggcaagcgcg
      481 acaccgaagc cataggtcag cgtaatcatg cgcgacacat tgatgccgaa ggcgcgcacc
      541 aaggtcggat tttcggtcgc ggcgcgcaga taggcgccga gccgcgtccg ctcaatcacg
      601 aaccaggtcc cgaagcagac caccagcgag gccgcaacca cccaggcgcg gtagttcggc
      661 aagaacataa agccgagatt ctgcccgccc cgcagcgaat ccggcacggt gtaaggcagg
      721 ccggccgaac cgaaataatt gcgcgcgacg ccttcaaaaa tcagcgccag cccgaatgta
      781 agcagcagcc catagaggtg gtccagcttg taaagccatt gcaggaaaaa ccgctcgacg
      841 agaatgccgg taatgccgac gagaaccggc gcaatgatca gtgcgggcca atatcccagt
      901 ccggaatatt ggagaataag ataagcggta aaagcgccca tcatgtattg ggcgccgtga
      961 ctgaagttga tgatgttgag catgccgaaa atcacggcca acccaagact caacagcgcg
     1021 tagaaggagc cgttgatcag gccgatcaac aactgtccaa acagcgcagt cgacgggatt
     1081 ccgaaaatct caaacatgca ggttaccgcg agatcggggg cggatgaaat cacccgcccc
     1141 tcgggtctcg tttttgccga ctagccgcta accagcgggc agttaccttc cttgagcgga
     1201 cggaaggcct gatcggcggg gatcgtcgcc cggaccttgt agaaatcgcc gggatgtttc
     1261 gattccgacg gcttcttcac ttcgagcaga taggccggat gcaccttgcg cccatccggg
     1321 cggaccgtgc ctttgccgaa aagcggatcg tcggtcggcg tcgacttcat ttgcgcgacc
     1381 accgctttgc cgtcgctcac attgcctttg agcgcgaccg cggccttgag gtagtgcgtg
     1441 atgccggaat agacgccggc atgaatcatc gtcggccact tgccttgacg ctcctgctgc
     1501 cagcgcttgg tccaggcgcg gttggcatcg ttcatgtccc agtaccaggt ttccgtcagg
     1561 accaggccct gtgcggtggg aagaccgagc gccgcgacgt cgctagcgaa caccagcaga
     1621 ccggcgaggc tctgcccgcc cttgacgatg ccgaactcgg ctgcctgctt gatcgagttg
     1681 atggtgtcgc cgcccgcgtt ggcaaggccg atgaccttcg ccttcgagga ctgcgcctgc
     1741 agcaggaacg acgagaagtc attggtgttg agcgggtgac ggaccttgcc gagcaccttg
     1801 ccgcccgcct tttcgactgc cgccgccgta tcgcgctcaa gcgcgtgacc gaatgcgtag
     1861 tccgacgtga ggaagaacca ggtgtcacca cccgtcttca cgatcgcggt gcccgtgccg
     1921 ttcgccagca tccaggtgtc ataggtccag tggatcgtat tggcgttgca cttcggaccg
     1981 gtgatgtccg aggcggccgc acccgacacg atgaacactt tgttcttgtt ggcggtgatc
     2041 tggctcacgg ccagtgccac accggaattg ggcacgtcga cgatcaggtc gaccttgtcg
     2101 acatcgtacc actggttcgc gatctgggcc ccgatgtcgg gcttgttctg gtgatcgccg
     2161 gaaatcagct caactttcac gttgggatga tctttggtga agtcggcgat ggccatcttg
     2221 gccgcggcaa ccgagccggg cccgccgatg tctgcataca ggctcgacat gtcgctgagc
     2281 acgccgatct tgacgttgac ctgctgggcg ttggcccctc cgcaggcaag aaccgcggcc
     2341 aaagccgcaa gtcccaaggt ttttcgcatc gattcattcc tccttgtttg actattcaga
     2401 tcgattagcc gctcacgagc gggcagttac cttccttgag cgga
//
LOCUS       DASSRL010000136         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_826193, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000136 DASSRL010000000
VERSION     DASSRL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826193"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFL49_02860"
     CDS             complement(<1..200)
                     /locus_tag="VFL49_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HET7910945.1"
                     /translation="MGRLTAIVLGSAAGGAFPQWNCRCPVCALAWAGDARVRPRTQTG
                     IAISAGDGRWTLVNASPDLGAQ"
     gene            complement(232..330)
                     /gene="pqqA"
                     /locus_tag="VFL49_02865"
     CDS             complement(232..330)
                     /gene="pqqA"
                     /locus_tag="VFL49_02865"
                     /inference="COORDINATES: protein motif:HMM:NF001400.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HET7910946.1"
                     /translation="MRPKGNAMVWTTPILIEICVGLEINGYLPAEF"
     gene            739..2730
                     /locus_tag="VFL49_02870"
     CDS             739..2730
                     /locus_tag="VFL49_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7910947.1"
                     /translation="MKSPRFNSLLASTAVALALAVSSHAGLAQQADTPAAASVPMPDT
                     TLPPPLTLKDIQPAATVDQPQQTATTPAAEPANAASTAIASGEIGDKLRELITSKQFD
                     RLVPRKADRAGIEAYYGGRNYAPLWVTNNAINDRAKTAAAYLAQADAVGLDPNDYPVP
                     SFKSGSSPDALAEDELKLTVSALTFARHAEIGRIHFSRVGSDIQYDLVPPDPASVLAK
                     LAESNDAGKVLDSYNPPQPEFKALKDKLAELRKGTLDTGPKTEAKPDKPRVHVPDGKI
                     LRPGMKDARVVAVRKRLDIAGDKDNPLYDDAVRDAVKTFQTESELEVDGNLGPNTTRA
                     LNGEQKEVRRPSHNPIDTILVNMERWRWLPRDLGNPHVIVNVPDYTLTLYNDGKVYWH
                     TKIVVGKPNLATPMVSAEMKFITVNPTWNVPPSIIEKEYLPALEQDPQALERIGLKLT
                     QDADGTIHISQPPGAGNALGRIRFNFPNKFLVYQHDTPDKYLFAREKRAYSHGCMRVQ
                     NPLTYGEKLLSLVLPKERYTEAKLESMFGGNEININFPKFIPVHLTYQTAFVDDAGKL
                     QLRDDVYGRDAKMLAILKSSERKVADIAVERAPNTSSKPVRMPVGMYGGPSGGYSYGG
                     GGGSNFFDWLFSGPQGRPYPPRPVPTNPRGNGNGRYSSR"
     gene            complement(2804..2880)
                     /locus_tag="VFL49_02875"
     tRNA            complement(2804..2880)
                     /locus_tag="VFL49_02875"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2844..2846),aa:His,seq:gtg)
     gene            complement(3034..3162)
                     /locus_tag="VFL49_02880"
     CDS             complement(3034..3162)
                     /locus_tag="VFL49_02880"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910948.1"
                     /translation="MRKQFDAFVTQAKDLSEHAQKVASETAEPIKGHFSNALNKAA"
BASE COUNT          682 a         1021 c          952 g          513 t
ORIGIN      
        1 atttgagcac ccagatcagg cgatgcattg acgagggtcc agcgcccgtc gcctgcagag
       61 atggcaatgc cggtctgcgt gcgcggacgc acgcgtgcgt cgccggccca cgccagagcg
      121 cagaccggac accggcaatt ccattgcgga aatgcacctc ccgccgccga accaagcacg
      181 atagcggtga gccgccccat ttagcggggt cgcaagggca ggatcgggta attagaactc
      241 ggccggaaga tagccgttga tttcgagtcc gacgcagatt tcgatgagga tcggggtcgt
      301 ccacaccatg gcgtttcctt tcggccgcaa atgaccagcg cggcgccaag attagcctag
      361 gccaagcccg ccgcaagggg gtgaccgacc gccccggcta ccgccaggca tcacgtcttt
      421 gtctgggttg tcatggttgc catgcgccgc cccgctatct cgggcataaa agcgcctcac
      481 gtgaaagtca tgggtataat cggcaaaaag ggcggcccga agcgcgcgcc ggaccggcgg
      541 ttgccatacg ttgattcgac gcggccttaa aaagacgcgc agacgggggt acctcaattg
      601 gtcatgcaaa ttagccacag tacccaattg ttcggcccgg gccgttggga aaaacgtaac
      661 tcgctaagga ctatcagtta aactggccgc gacatataag ggcgcgcctg ccctgtgaca
      721 gctttgggag ataaagtggt gaaaagtccc cgatttaaca gcctgctggc ctcgaccgcc
      781 gtggcgctcg cactggccgt gtccagccat gcaggcctgg cgcagcaagc cgacacgcct
      841 gccgccgcat cggttccgat gccggacacc acgcttcccc cgccgctgac gctgaaggac
      901 attcagccgg ccgcgaccgt tgatcagccg cagcagacgg ccacgacgcc ggcggccgaa
      961 ccggccaacg cggcatcgac ggcaatcgcc agcggtgaaa tcggcgacaa actccgcgaa
     1021 ttgatcacca gcaagcagtt cgaccggctg gtgccgcgga aagccgaccg cgccgggatc
     1081 gaggcttatt atggtggccg caactatgcg ccgctctggg tgaccaataa cgccatcaac
     1141 gaccgcgcca agacggcggc cgcctatctt gcgcaggccg acgcggtcgg tctcgacccg
     1201 aacgactacc cggtcccaag tttcaaatcc ggaagcagcc cggacgcgct ggccgaggac
     1261 gagctcaaac tgacggtgtc ggcgctgacg tttgcgcggc acgcggagat cggccgcatc
     1321 catttcagcc gcgtcggttc cgacatccag tacgacctgg tgccgcccga tccggcgagc
     1381 gtcctggcaa agctcgcgga gagcaacgat gccggcaaag ttctcgacag ctacaacccg
     1441 ccccagccgg aattcaaggc gctaaaggac aagcttgctg aattgcgcaa gggcacgctc
     1501 gacaccggcc cgaagacaga agcgaagccg gacaagccac gcgtccatgt gcccgacggc
     1561 aagatcctgc gcccgggtat gaaagacgcg cgcgtcgtgg cggtgcgcaa gcggctcgac
     1621 attgccggcg acaaggacaa tccgctttac gacgacgcgg tccgcgatgc ggtcaagacg
     1681 ttccagacgg aaagcgagct ggaagtcgat ggcaatctcg gcccgaacac gactcgcgcc
     1741 cttaatggcg agcaaaagga agtgcgccgc ccgagccaca atccgatcga taccatcctc
     1801 gtcaacatgg agcgctggcg gtggttgccg cgcgacctcg gcaacccgca cgtgatcgtc
     1861 aacgtcccgg actatacgct gacgctttac aacgacggca aggtgtactg gcacaccaag
     1921 atcgtggtgg gtaagccgaa cctcgccacg ccgatggtca gtgccgagat gaaattcatc
     1981 acggtcaacc cgacctggaa tgtgccgccg tcgatcatcg agaaagaata tctgcctgct
     2041 ctcgagcagg atccgcaggc gctcgaacgc atcggcctca agctgacgca ggacgcggac
     2101 ggcacgatcc acatttcgca gccacccggc gcaggaaacg cgctgggtcg catccgcttc
     2161 aactttccca acaagttcct cgtctaccag catgacacgc ccgacaagta tttgttcgcg
     2221 cgtgagaagc gcgcctacag ccacggctgc atgcgggtgc aaaatccgct gacatacggg
     2281 gaaaagctgc tgtcactggt gctcccgaag gagcgctaca ccgaagcgaa gctcgagagc
     2341 atgttcggcg gcaacgagat caacatcaac ttcccgaagt tcatcccggt gcacctcacc
     2401 taccagacgg ccttcgtgga cgatgccggc aagctgcagc tgcgcgacga cgtctacggc
     2461 cgcgatgcca agatgcttgc aatcctcaag agcagcgagc gcaaggtcgc cgatatcgca
     2521 gtcgaacgcg caccaaacac atcgtcgaag ccggttcgca tgccggtcgg aatgtacggc
     2581 gggccgagcg gcggctacag ctatggcggc ggtggcggtt caaacttctt cgactggctg
     2641 ttcagcggcc cgcagggacg tccttacccg ccgcgtccag tgccgaccaa tccgcgtggc
     2701 aatggcaacg gccgctacag ctcgcgctag aatacgaaca gactcccaag cagaacgccg
     2761 gcgcatcacc gccggcgttt tcttttcgcg gtacgtgagg aagtggtaca ggcgggtgga
     2821 cttgaaccac cgacctctgg ttccacagac cagcgctcta accaactgag ctacgcctgc
     2881 acacgcgatc ggctttgttc aagcgcgatc caagcggccg gaacctaggt gccgcccgcc
     2941 attttggcaa gcggctggtc acaccgcaaa acaaaagccc ggacattccg gtccgggcct
     3001 tgaactcgct gcttgttcag gacgacgcga gcgtcaggcg gccttgttca gcgcgttgct
     3061 gaagtggccc ttgatcggct ccgcagtttc gctcgcaact ttctgcgcgt gctcggagag
     3121 gtccttggcc tgagtgacga acgcatcaaa ttgcttgcgc agatagac
//
LOCUS       DASSRL010000137         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1333489, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000137 DASSRL010000000
VERSION     DASSRL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333489"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFL49_02885"
     CDS             <1..295
                     /locus_tag="VFL49_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HET7910949.1"
                     /translation="AATIAELVIIAYRDGQDIGGSDVMSRYDRMRRADVGSRTLAIDL
                     LNRSLLSDLLPVQGARGLGLYLLDQVGPLRRAIMREGVAPAARQPRLMRGEAL"
     gene            complement(292..1380)
                     /locus_tag="VFL49_02890"
     CDS             complement(292..1380)
                     /locus_tag="VFL49_02890"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7910950.1"
                     /translation="MQPLTGLFVLDFTTLLPGPLATLMLAEAGAEVLKIEQPEGENAR
                     RFPPFVDGESAAFVMLNRGKTSMALDLKSNAAREKLIPLIERADIVVEQFRPGVMDRL
                     GLGYDGLKKINPRLIYCSISGYGQSGPRVHEAGHDINYIGNTGLLDLQPGPADRPVVP
                     PMLAADIAGGSFPAVINILLALRARDQSGKGCRIDIAMTDAMFTFTWAALALGVATGR
                     FPKSGDLWLVGGSPRYQIYPAKDGKLIACGALEQKFWVAFTSAIGLPKQFVDDARDPM
                     ATRDTIAKLILARTSDEWRRILADADCCATIIIPLEQAMRDPHFVERGLFAHHVGTES
                     GKTLPALPLPIAPEFRAAPGTKKAPKLR"
     gene            1500..>2029
                     /locus_tag="VFL49_02895"
     CDS             1500..>2029
                     /locus_tag="VFL49_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7910951.1"
                     /translation="MRQLPHMGIVLGMHAHTFPDKIGARDLEREMTFRTWHERSCRLA
                     NALTGLGLAKGERVCVLAYNCVEWLEIYAATALAGLVAVPVNFRLLGHEIRHIVEDCD
                     ASAIIVQDQLIDAVESVRDTLPVAAGRFIVFGTDRRHAGYRAYEGLLAAASDRPPSVT
                     VTPGDPWTLMYTSGTTG"
BASE COUNT          364 a          646 c          633 g          386 t
ORIGIN      
        1 tgccgcgacg atcgccgagc tcgtcatcat tgcgtacaga gacgggcaag acatcggcgg
       61 atccgacgtg atgtcccgct acgaccgcat gaggcgcgcc gatgtcggca gccgcacgct
      121 cgctatagat cttctcaacc gctcgctgct gagcgacctg cttcccgtgc agggcgcgcg
      181 cggccttggc ctctatttgc ttgaccaggt cggtccgctc cgccgcgcaa tcatgcgcga
      241 aggcgtcgca ccggctgccc ggcagccgcg cctcatgcgc ggcgaagcgc tttagcggag
      301 cttcggcgct tttttcgttc cgggagcggc gcgaaactcc ggcgcgatcg gcagcggcag
      361 cgccggcagc gtctttcccg actccgttcc cacgtgatgc gcgaacagcc cgcgttcgac
      421 gaaatgcggg tcgcgcatcg cctgctcgag cgggatgatg atcgttgcgc aacaatcggc
      481 atccgccagg attcgccgcc actcgtccga tgtcctggcc aaaatcagtt ttgcaatggt
      541 atcgcgggtt gccatcggat cgcgcgcgtc gtcgacgaat tgtttcggca gcccgatcgc
      601 agaagtgaat gccacccaga atttctgttc cagcgcaccg caagcgatca gcttgccgtc
      661 tttcgccgga tagatttggt aacgcggcga tccgccgacg agccacaagt cgcccgactt
      721 cggaaagcgc cccgtcgcca cgccaagcgc caatgccgcc caggtgaacg tgaacatcgc
      781 atcggtcatc gcaatgtcga tcctgcagcc ttttccgctc tggtcgcggg cccgcagggc
      841 aaggagaata ttgatgaccg cggggaagct gccgccggcg atgtcggccg ccagcattgg
      901 cggcacgacc ggccgatcgg cgggacccgg ctggagatcg agcaggcctg tgttgccgat
      961 atagttgatg tcgtgccccg cctcgtgaac gcgcggtccg ctctggccgt agccggagat
     1021 tgagcaatag atcaggcgag gattgatctt tttcagtccg tcgtagccga gaccgagccg
     1081 gtccatcacg cccgggcgga actgctcaac cacaatgtcg gcgcgctcga taagcgggat
     1141 aagcttttcg cgagcggcat tacttttcag atcaagtgcc atgctcgtct tgccacgatt
     1201 gagcatgacg aaggccgcgc tttcgccatc gacgaaaggc ggaaagcgtc gcgcattttc
     1261 accctccggc tgctcgatct tgagcacctc cgcaccggct tcggcgagca tcaatgttgc
     1321 caatggtccg ggaagcagag tggtgaaatc caggacaaaa agaccggtca gcggctgcat
     1381 cacaatttga cgtacctcgc gaatgccgga gtttgtcatg cccgacgcgc ggcgtcacgc
     1441 attgccagac ccatccgccg cgggcacagt atctttgatc ggttgtggag tagaattgga
     1501 tgagacagct gccgcacatg ggaatcgtgc tcggcatgca tgctcataca tttcccgaca
     1561 agatcggagc gcgcgatctt gagcgggaga tgaccttccg gacatggcat gagcgctcgt
     1621 gccgtctcgc caacgcgctg acggggctcg gtctggccaa gggcgagcgg gtttgcgtgc
     1681 ttgcctacaa ctgcgtcgaa tggctcgaga tttacgccgc gacggcactg gcgggcctgg
     1741 ttgcggtgcc ggtcaacttc cggctgcttg gccacgagat ccgccacatc gtggaagact
     1801 gcgatgccag cgcgatcatc gtccaggatc aattgatcga tgcggtcgag tcggtccgcg
     1861 acacgcttcc cgttgcggcc ggcagattca tcgtcttcgg caccgaccgc cgccatgccg
     1921 ggtaccgcgc ctatgagggc ctgcttgcag cagcaagcga tcgtccgccg tccgtgaccg
     1981 tgacgcccgg cgatccgtgg acgctgatgt acacctcggg cacgaccgg
//
LOCUS       DASSRL010000138         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1631081, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000138 DASSRL010000000
VERSION     DASSRL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631081"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFL49_02900"
     CDS             <1..670
                     /locus_tag="VFL49_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910952.1"
                     /translation="PAIYPKLPYAMSDLAGITQTAVSKYVLVVPLSSSAKSVRDLVAL
                     AKAKPRQLNFSSAGVGSGTHFAGEMFKYSAGIDVVHVPYRGISETLTDLMAGRAQFFM
                     APIGSASQLVRDGKLRGLGVSTLVRSPALPDIPTIAESGLPGFEWDSWGSLLAPARTP
                     RAIIERWNQEIRRAGSAPDVTKRLNAIGMSAAPSGARELEGLIKQQMAVVAKLVKAAN
                     LKSE"
     gene            complement(900..1250)
                     /locus_tag="VFL49_02905"
     CDS             complement(900..1250)
                     /locus_tag="VFL49_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET7910953.1"
                     /translation="MRSSIRRYASTHGSVLLESAYEACIEYELGKRSLAVRKQVPMPL
                     RYDDLALDIGFRLDLIVEDAVVIELKSVKQLLPVHTAQLLSYLRAGDFRIGFLLNFNT
                     VHMRDGIKRVVNGL"
     gene            complement(1554..>2475)
                     /locus_tag="VFL49_02910"
     CDS             complement(1554..>2475)
                     /locus_tag="VFL49_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7910954.1"
                     /translation="AAIAAQVTAPALGQNYPSKPIRIVTSPAGGGNDFPARLISKYVS
                     GPLGQQIVVDNRPTILIADIVAKSPPDGYTLLVSGSAHWIGPLLEKVTYDPIKDFAPI
                     TLIDRSPSVLVVHPSMPVKSVKQFISLAKAKPDQMNFSVGGPGSSGDLGALLFNHMAG
                     VKILRIPYKGTSPALIAVMSGEAHAMFGSPGSVAPHVAAGRLRALAVGSAQPSKLAPG
                     LPTIAQSGVPGYLSEALHAFFAPAGTPPAIISLLNREVGNYLRSNEAQAIFLKAGVEP
                     SPGTPEEFTAVMKSEMERIGAVLKAPESRR"
BASE COUNT          457 a          773 c          789 g          456 t
ORIGIN      
        1 accggcgatc tatccgaagc ttccgtatgc gatgagcgat cttgccggga tcacgcagac
       61 tgccgtgtcg aagtacgtcc tcgtcgtgcc gctctcgtcg agcgcgaagt ctgttagaga
      121 cctcgtggcg ctggcgaagg cgaagccccg gcagctcaat ttttcctcgg cgggtgtcgg
      181 cagcggcacg catttcgccg gcgagatgtt caagtacagc gccgggatcg acgtcgtgca
      241 cgtgccttac cgcggcatct cggagacgct gaccgacctc atggcgggcc gcgcgcaatt
      301 cttcatggcg ccgatcggga gcgcttcgca gctcgtgcgc gacggcaagc ttcgcggtct
      361 cggcgtctcc accttggtgc gctcgcccgc actgccggac atccccacca tcgcggagtc
      421 cggcctgccg ggcttcgaat gggattcctg gggctcgctc ctcgcgccgg cgcggacgcc
      481 gcgcgcgatc atcgagcggt ggaatcagga gatccgccgc gcgggaagcg cgccggacgt
      541 caccaagcgg ctgaacgcga tcgggatgag cgcggcgccg tcaggcgctc gcgagcttga
      601 ggggctgatc aagcagcaaa tggcggtcgt agcgaagctt gttaaagcag caaatctgaa
      661 gtcagagtag tgcggcgtgg aaaagcctca acgcgaagga cgcgaaggtc acaaaggaaa
      721 agaaaagcct caacgccaag gacacgaagg acgccaagga acggaacagc ctcaacgcaa
      781 aggccgcaaa gatcgcgaag gatctaataa taagaagatc aacttcaaca ccggaagacg
      841 cgaaggagct gcgccaagcg tggcaattgc aacgcctcgg cattctagct ggcgaagctt
      901 tacagcccgt ttacgacgcg cttgatgccg tcgcgcatgt ggacggtatt gaaattgagc
      961 agaaaaccga tacggaagtc gccggcacgg aggtagctca gcagttgcgc ggtgtggacc
     1021 ggtagcaact gcttgacgga tttcaactcg atcaccacgg cgtcttcgac aatcaggtct
     1081 aggcgaaaac cgatgtcgag tgcgagatcg tcgtatcgca acggcatcgg aacttgcttc
     1141 ctgactgcga gcgagcgctt gccgagctcg tattcgatgc aggcctcata agccgattcc
     1201 agcaggaccg atccgtgagt ggacgcgtat cgccgtatcg acgatctcac gggcaatcgg
     1261 ttcgacgtcc acgggattct ccggataacg gttctgaacc gtttaacgga caagacccaa
     1321 tggagttgac gcggcacgcg cgtcatgggg tgtgaggcgc tacgtcaacc acggacgctg
     1381 gggacacccc ccttggcgtt gtcttagcgt cctttgtttt taatatatat tttcttcgcg
     1441 acctttgcgg cctttgcgtt gaggcttttc ttttccttcg cgtcctttgc gcccttagcg
     1501 ttgaggcttt tctgttcctt ggcgccctta gcgtccttcg cgttgaagct tttctaccgc
     1561 cgcgactccg gcgccttcag cactgcgccg attctctcca tctccgactt catcaccgcc
     1621 gtgaactcct ccggcgtgcc cggcgacggc tcgacgcccg ccttgaggaa gatcgcctgc
     1681 gcctcgttcg agcgcaggta gttgccgacc tcccggttga gcagcgagat gatcgcgggc
     1741 ggcgtgcccg ccggcgcgaa gaacgcgtgc agcgcttccg acagatagcc cggcacgccc
     1801 gactgcgcga tcgtcggcag ccccggcgcg agcttcgacg gctgcgcgct gcccaccgcg
     1861 agcgcgcgca ggcgtcccgc cgccacgtgc ggggcgacgc tgcccggaga gccgaacatc
     1921 gcgtgcgctt ccccgctcat cacggcgatc aacgcgggcg acgtcccctt atacgggatc
     1981 cgcaggatct tcacgcccgc catgtggttg aagagcagcg cgccgagatc gcccgagctg
     2041 cccggcccgc cgacggagaa gttcatctgg tcgggcttcg ccttggcgag cgagatgaac
     2101 tgcttgaccg acttgaccgg catcgacggg tgcacgacaa gcacgctcgg cgaccggtcg
     2161 atcagcgtga tcggcgcgaa atccttgatc gggtcgtagg tcaccttctc gagcaatggc
     2221 ccgatccagt gcgcgctacc gctgacgagc agcgtgtagc cgtccggcgg cgacttggcg
     2281 acgatgtcgg cgatgaggat cgtgggccgg ttgtccacga caatctgctg tccgagcggc
     2341 ccggagacgt acttcgagat cagccgcgcc gggaaatcat tgccgccgcc tgccggcgaa
     2401 gtgacgatgc ggatgggctt ggatggatag ttctggccga gcgccggcgc ggttacctgc
     2461 gccgcgatcg cggct
//
LOCUS       DASSRL010000139         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_966088, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000139 DASSRL010000000
VERSION     DASSRL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966088"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFL49_02915"
     CDS             complement(<1..274)
                     /locus_tag="VFL49_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7910955.1"
                     /translation="MAGTPKILRTVPSLRRTLEPYRKATAGIALVPTMGALHRGHMAL
                     IRAARQRAKRVVVSIFVNPTQFAPHEDFASYPRNFAADIEALREANV"
     gene            425..1345
                     /locus_tag="VFL49_02920"
     CDS             425..1345
                     /locus_tag="VFL49_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HET7910956.1"
                     /translation="MLIQQIQETVQSAHVVVLGNEKGGSGKSTTALHIAVALLKAGQR
                     VATIDLDSRQQSFTHYIDNRRAWAERARVSLDLPTHYCIARAEGNSLEANEAREFENF
                     SAAIGAIEHNHDVVVIDTPGADTYLMRLAHSMADTLVTPLNDSFLDFDVLATLDPTNF
                     TVIGESHYAEMVREARRQRRLVDGRLTDWIVVRNRLSNLVSRNKRLVGDGINELSKRM
                     GFRCVEGFAERVVYREFFPRGLTALDDLDEETLGTRPNLSHVTAREEVISLLNALQLP
                     LSENGKRRAAARAEWFSVMDQPLQVHDIIG"
     gene            1438..2148
                     /locus_tag="VFL49_02925"
     CDS             1438..2148
                     /locus_tag="VFL49_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7910957.1"
                     /translation="MAKDSRQTPAPVSSRSEIDAFLERVRTLGPSSAQGTRGRLVFAL
                     DATMSRQPTWDTACMLQADMFREAAGIGGLDIQLVYFRGLNECRASGWVSGGKQLADL
                     MSRIGCRGGQTQIGKVLNHARQEHANGKVQALVFVGDAMEEAIDDLCQAAGELGILGV
                     PVFMFQEGGDPIAENAYREIARLSRGAYCRFDTGSAHQLGELLRAVAAYAAGGLRALK
                     DLSARRSSGAQKLLAQLK"
     gene            2150..2851
                     /locus_tag="VFL49_02930"
     CDS             2150..2851
                     /locus_tag="VFL49_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7910958.1"
                     /translation="MPALIFGVLVLVLALWALSVISKVDPKIAARVTKIGGGMLSLGL
                     AIFLGLRGEVGVAIPLGAFGLGLLGWVPFGPAGFGARTQKAGGQTSRVRSTYFDMELD
                     HDSGTMRGHVIAGRYQGAELNRLDVETLIAMLGEIDEESRALLVAYLDRRDPGWSENP
                     QADAAAGRRAAASGKMSEQEAYQILGLESGASADDIGRAHRTLMKKLHPDQGGSTYLA
                     ARINEAKEILLRRHR"
     gene            complement(2921..>3361)
                     /locus_tag="VFL49_02935"
     CDS             complement(2921..>3361)
                     /locus_tag="VFL49_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7910959.1"
                     /translation="NITSVITSKNGQPPTIVPSVPASPTPEAFVAKTDPDPKSAPATK
                     MASAAPSVPIPTPQPEPAPKQHTGWIIQIGALPDEKDAWQRLELAQTKAKNILGKADP
                     FTERVEKGEKVLFRARFAGLEKNQAEAACKHLKRSDIPCMMLKN"
BASE COUNT          657 a          909 c         1063 g          732 t
ORIGIN      
        1 caacgttcgc ctcgcgcaac gcttcgatat cggccgcgaa gttgcgcgga tagctcgcga
       61 agtcctcgtg cggggcaaat tgtgtcggat tgacgaaaat agagacgaca acgcgcttgg
      121 cgcgttgccg tgccgcgcgg atcagagcca tgtggccgcg atgcaacgcg cccatcgtgg
      181 ggaccagtgc gataccagcg gtcgcctttc gatacggttc caacgtacgg cggagcgaag
      241 gaaccgttcg caaaatcttc ggagtgcctg ccatgatttt ccggccttgt cggtgaagtg
      301 ttgcaaaacc acaatacgtc gagcaaggcg gcgaagatga cccaagctag ttgaggatca
      361 aatcgggatt aacttgacta agctatccga atattttgac aattggcagc cggagagggg
      421 cggcatgctg atccagcaga tccaggagac cgttcaatca gcgcatgttg tcgttctcgg
      481 aaacgagaag ggcggttcgg gaaaatcaac aactgcgttg catatcgctg ttgcgctgct
      541 aaaggccggg cagcgtgtcg caacgattga tctcgattcg cgtcagcaaa gcttcacgca
      601 ctacattgac aatcgccgtg cttgggccga gcgcgcacga gtgtcgctgg atttgcccac
      661 gcactattgt attgcacgtg ccgaaggcaa ttcgctcgaa gccaacgagg ccagagaatt
      721 tgaaaatttc tcggcggcga tcggggcgat tgagcacaat cacgatgtcg tcgtcatcga
      781 tacgcctgga gcagacacgt atttgatgcg gttggctcat tcgatggccg acactttggt
      841 gacgccgcta aacgacagct tcctcgactt cgacgtgctc gccacgcttg atccgaccaa
      901 ttttacggtg atcggggaaa gccattatgc ggaaatggta cgggaggcgc gccgtcagcg
      961 ccgactggtt gacggccgtc tcactgattg gattgtcgtg cggaaccggc tgtccaatct
     1021 tgtctcgcgt aacaaacggc tggtcggtga cggaatcaac gagctctcga aacgaatggg
     1081 ctttcgatgt gtcgaaggct ttgcagaacg tgtcgtttat cgtgaatttt tcccgcgagg
     1141 gttgaccgct ctcgacgacc tggacgagga aacacttggt acgcggccaa atctctcgca
     1201 tgtgacagcg cgcgaagaag tcatttcgct gctcaatgcg ctgcaattgc cactttcgga
     1261 gaacggtaaa cggcgcgcgg cggcccgtgc tgaatggttc tcggtcatgg accagccact
     1321 gcaggttcac gacattatcg ggtaggccgc cagcttgcat ttttgctctc gggtcgccat
     1381 ctggcgcgat gcgaggacag acgcgcgagc gacggtgatt cagtgtagaa tcgcaccatg
     1441 gccaaggact cccgacagac gcctgcgccg gtttcgtcca ggtccgaaat tgacgcgttt
     1501 ctggaacgtg ttcgcacgct cgggccgtca agtgcgcaag gtacccgcgg tcgtctggtc
     1561 ttcgcgctcg atgcgacgat gagccggcag ccgacctggg acacggcttg catgctgcag
     1621 gctgacatgt ttcgggaggc cgccggtatt ggcgggctcg acattcagct ggtctatttt
     1681 cgcggtttga acgaatgccg cgcgtcagga tgggtgtccg gggggaagca gctcgcggat
     1741 ttaatgtcca ggatcggctg tcggggaggg cagacgcaga tcggaaaagt tctcaatcat
     1801 gcccgccagg aacatgcgaa cggcaaggtt caggctcttg tcttcgttgg cgatgccatg
     1861 gaggaggcaa tcgacgatct gtgccaggcg gccggtgagt tgggcattct cggagtgccg
     1921 gtcttcatgt ttcaggaagg cggagacccg atcgccgaga acgcatatcg tgagatcgcg
     1981 cgcctgtcgc gcggtgcgta ttgccggttc gataccggct ccgcacatca gctcggtgaa
     2041 ctgctgcgtg cggttgccgc ttatgccgca ggtggccttc gcgctctcaa ggacttgtcg
     2101 gccaggcgct cgtccggcgc gcaaaaatta ctggcgcaac tgaagtaaca tgcccgctct
     2161 catcttcggc gttctggtgt tggtgctcgc gttgtgggcg ctgagcgtca tctccaaggt
     2221 tgatccgaaa attgccgcgc gggtgacaaa gatcggcggc ggcatgcttt cgttggggct
     2281 tgcgattttc ctgggcttgc gcggagaagt tggcgtagca attccgttag gtgcctttgg
     2341 gctcggcttg ctcggatggg tgccgtttgg acccgcgggt tttggcgcgc gtacgcaaaa
     2401 ggcagggggg cagacctcgc gcgtgcgctc gacctatttc gacatggaac tcgatcatga
     2461 cagcggcacg atgcgtggcc acgtcattgc cgggcgttat caaggcgccg agctaaatcg
     2521 gctggacgtc gagacgctga tcgcgatgct tggcgagatc gatgaagaga gccgtgcctt
     2581 actcgtggcg tatcttgacc gccgggatcc cggctggagt gagaacccgc aagcggatgc
     2641 ggcagcgggg cggcgcgcgg cggccagcgg aaaaatgtcc gagcaggagg cctatcagat
     2701 ccttggcctg gagtccggcg cgagcgccga tgacatcggc cgcgcgcacc gcacgttgat
     2761 gaagaaactg catcccgacc agggggggtc gacgtatctt gccgcccgca tcaacgaagc
     2821 caaagaaatc cttctccgcc gtcatcgctg aggtactcca caaacacgac catcagcggc
     2881 tcctggttcg gcacgtccct gttattcaat ttttggaggt tcaattcttc agcatcatgc
     2941 atggaatatc gctgcgcttg agatgtttac aggcagcctc tgcctggttc ttttccaatc
     3001 cagcgaagcg cgcgcgaaac agcacctttt cgcctttctc aacacgttcg gtgaatggat
     3061 cggccttgcc gagaatgttt ttggcctttg tttgagccag ttccaaccgc tgccatgcat
     3121 ccttctcatc cggcagcgcg ccaatttgaa taatccatcc ggtatgctgc ttgggcgccg
     3181 gctcgggctg gggtgtcggg atggggacgc tcggagcagc ggacgccatt tttgtggcag
     3241 gcgcggattt aggatcagga tcagttttcg cgacgaaggc ctcaggcgtt ggcgatgcag
     3301 gaacagatgg cacgattgtc ggaggctgtc cgttcttcga ggtgatgacg ctggtaatat
     3361 t
//
LOCUS       DASSRL010000140         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_143924, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000140 DASSRL010000000
VERSION     DASSRL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143924"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            852..1205
                     /locus_tag="VFL49_02940"
     CDS             852..1205
                     /locus_tag="VFL49_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910960.1"
                     /translation="MRYGIARQVFVCLLALAVATAGVARVVRADNMHVAIAAGGMATD
                     MPVHSKCSGCAGDEKGLASVACSVYCASVVALPAMPAATNVAVAEVLRPSDQLRLAGL
                     AAPPDPYPPRTIILS"
     gene            1340..2818
                     /locus_tag="VFL49_02945"
     CDS             1340..2818
                     /locus_tag="VFL49_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HET7910961.1"
                     /translation="MAAHFSFAITRRSVLLGAAGTAAAGALSKTGVASVAVREIRLAA
                     GPSRWPLVGKGRPEANVWCYDSRIPGPEIKIRQREPVRIVVENRLSEDTTVHWHGIRL
                     PNSMDGVPGLTQPPIKPGDKFVYEFTPPDAGTFWYHPHADSLRQLGRGLAGALIVSEA
                     ASVSVDRDILWMLADWRLTPDAQIAPGFGNAMEAAMSGRVGNTVTLNGAIPTDEHVRA
                     GERVRLRLVNSALARIMALRFEGHRPVVVAIDGQPCDPHEPKAGRVLLGPAMRIDLIL
                     DMQGEPGKRYRVIDDFYPELSYWLTNLTYGKEPPVRAHPLDAQVILPGNPLPQPDLTT
                     AERHELKLQGGMMSGMGGMMGSGGMMGGGMMGGGMNGTGGMMGMSGGATWSINGTSMT
                     GDGHANMQPLLTLTRGRTYILTLRNETAWWHPMHLHGHSFRVLSRNGDSVPNKEWADT
                     VLVPPKETADIAFVADNPGDWMLHCHVTDHQVSGLMTVLRVT"
     gene            2850..3302
                     /locus_tag="VFL49_02950"
     CDS             2850..3302
                     /locus_tag="VFL49_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HET7910962.1"
                     /translation="MKRRTFCLACMSVAVTAFAGQLPAAAETTRAILYKNPECTCCEG
                     YAAYLRQNGFEVDIKATNDLTEISRNAGVPENLQGCHTMFVDGYVVDGHVPIKIVKKL
                     LSERPAIAGITLPGMPLGSPGMSGQKVGTFTIYAVTKNGKAPTVYATD"
     gene            3391..3927
                     /locus_tag="VFL49_02955"
     CDS             3391..3927
                     /locus_tag="VFL49_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7910963.1"
                     /translation="MRYRFAAGFAAVLLLAATSQSAGAQAPPKNFIVHAAPTPIAAIG
                     FQDDQGHAKTLADFKGKVVVLNIWATWCTSCRTEMPALDRLEADLGGPKFAVVALSID
                     RGGISSIEKFYAATGISHLPKYVDSSGQVLRGLGALGLPTTLLLDREGNEVGRVVGAV
                     EWDAADTIAYLKSVIAER"
     gene            3942..>4184
                     /locus_tag="VFL49_02960"
     CDS             3942..>4184
                     /locus_tag="VFL49_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910964.1"
                     /translation="MTPSSQTTNNSFARDLVSVAQYYLRPRWVLLGIAIAAIAIGLTL
                     NWSWLVAAGIAPILLAMLPCLVMCGLGLCMHKMVGAS"
BASE COUNT          860 a         1278 c         1265 g          781 t
ORIGIN      
        1 tcaatgcgtt agccggacgt attatccggc ccaacgccgt ccctgccgac accccgccac
       61 gtagaagtca ttaagtgttc tggagatcct ggctgggctg ttgtggggga tatttcggta
      121 gggttggcaa actcgccaac aaatcagtct tagctcatga gtgggcgaag cctaactgcc
      181 aatccggccg cgcagtcgaa gatcgaacca gtctccgctc ccgtctccgc aaaacgggaa
      241 ttcttcagat atcgaccaga gactttccgc gaatacagcg aatttcgcct cggcgcaggc
      301 cacctcagag accggcgcag ccacgaaaag gaccgcacgt gcgcgccctt ttccagcgcg
      361 cgatgggtaa aaccggggag accggactga ctggctgggg tggcaggatt cgaaccgcca
      421 aatccccttt tcgaaattgg cgtttgaaac tccgccagaa tttcgggttt tttgcgccag
      481 tttggggtcc agagactttc acgcgacaaa gttgctggaa cggacaagga caccctgcat
      541 cacagcgaaa tcaacggcgg gctaagagcg ggagaggcgt ccgtcagcgc ggcacagacc
      601 tgaggccgga cgccaagaac gactgggcga tgacccaccc agtgctcgat cgaactgggt
      661 ctccgccaac caattcacga gtttgggaat atctggcaag accgtaaaca gcatcggccc
      721 cagagaccgt ctcgtagacc ggtccgcttt acacaccgaa aggccacatt ccatacaccg
      781 caatgttgcc acgttgaagc aggacattgc ggtatatggc gtcaaatgac gcgatctgct
      841 aacttcaaca aatgcgatac ggaatcgcgc gccaagtatt cgtgtgtctt cttgcgctgg
      901 ctgtcgccac cgcaggggtg gctcgcgtcg tccgggccga caatatgcat gtcgcgatcg
      961 ccgccggtgg catggccacc gacatgcccg tgcacagcaa atgcagcggc tgcgccggcg
     1021 acgaaaaggg cctggcgtca gtggcgtgct ccgtttactg cgccagcgtc gttgcgttac
     1081 ccgcaatgcc agcggcaaca aacgtggcgg tcgccgaagt cctgaggcct tccgatcaat
     1141 tacgtctggc gggccttgcg gccccacccg atccctatcc gcccagaact atcatcctga
     1201 gctgaagcac gctgctttcg ggcagcggtg acttcatgtg cgctcgtgac gcatgtcgcg
     1261 tcacccgcat cacaggcacg agcactgata tcgcttcggc atcgtgttgt gccggacctc
     1321 tcctacagga tgactgtgaa tggctgctca ttttagtttt gcgataacgc gccgctccgt
     1381 gcttctcggc gccgccggca ctgccgcggc cggagccctg tcgaaaacgg gtgttgcttc
     1441 ggtggcggta agagaaatca ggctggccgc cgggccttcc cgatggccat tggtcggcaa
     1501 gggtcggcct gaggcaaacg tatggtgcta cgacagccgc attcccgggc cggaaatcaa
     1561 aatccggcag agagagccgg tccgcatcgt cgttgaaaac cggctgtctg aagatacgac
     1621 cgtgcactgg cacggcatcc ggctgccgaa ttccatggac ggggtgcccg gcctcaccca
     1681 gccgccgatc aagccgggcg ataaatttgt ctacgaattc acgccgcccg acgcgggcac
     1741 gttctggtat cacccgcatg cggacagcct gcgccagctc gggcgcgggc tcgccggcgc
     1801 cctgatcgtc tcggaagcag catcggtttc cgtcgatcgc gacattctct ggatgcttgc
     1861 cgactggcgg ctgacgccgg acgcgcagat tgcgcccggc ttcggcaatg ccatggaagc
     1921 ggcgatgtcc ggacgggtcg ggaatacggt gacgctgaac ggcgccatcc ccaccgacga
     1981 acatgttcgc gcgggagagc gcgtgcgatt gcggctcgtc aacagcgcac ttgcacgcat
     2041 catggcgctg cgtttcgagg ggcatcggcc ggtggtggtg gcgatcgacg gccagccctg
     2101 cgatccgcac gagccgaaag cgggccgcgt gctgctcggc ccggccatgc gcatcgacct
     2161 gatcctcgac atgcagggcg agccgggaaa acgctatcgg gtcatcgacg atttctaccc
     2221 cgagctctcc tattggctga ccaatctgac ctacggcaaa gaaccgccgg ttcgtgcgca
     2281 tcctctcgac gcgcaagtca tcctgccggg caatcccctg ccgcagcccg atctcaccac
     2341 agccgaacgt cacgagctca agctgcaagg cggcatgatg agcggaatgg gcggcatgat
     2401 gggtagcggt ggcatgatgg gtggcgggat gatgggcggc ggcatgaatg gcacgggcgg
     2461 gatgatgggt atgagtggcg gcgcaacctg gtccatcaac ggcacgtcaa tgaccggcga
     2521 cggtcacgcc aacatgcagc cgctgctgac gttgacacgc gggcgaacgt acatcctgac
     2581 gctgcgcaac gagacggcct ggtggcatcc gatgcatctg cacggacaca gcttccgcgt
     2641 gctcagccgc aacggtgatt ccgttccaaa caaagaatgg gcggacaccg ttcttgtgcc
     2701 gcccaaagaa acggccgata tcgccttcgt cgccgacaat cccggcgact ggatgctgca
     2761 ttgtcacgtc acggatcatc aggtctcggg cctgatgacc gttctgcgcg ttacctgaac
     2821 ttgtccattt caaaaaaagg agccgctcca tgaaacgacg gacgttttgc ctagcctgca
     2881 tgagcgtcgc tgtaacggca tttgccggac agctaccggc cgccgccgaa acaacccgcg
     2941 cgatcttgta caagaacccg gaatgcacgt gctgcgaggg ttatgccgct tatttgcgcc
     3001 agaacggctt cgaggtcgac atcaaggcaa caaacgatct gaccgagatc agccgcaatg
     3061 ccggagtgcc cgaaaatctt cagggctgcc acacgatgtt cgtggacggc tacgtggtcg
     3121 acggccacgt gccgatcaaa atcgtgaaaa agctcttgtc cgaacgtccg gccattgcag
     3181 gcatcactct gccggggatg ccgcttggat cacccggaat gagcgggcaa aaagtaggaa
     3241 cattcaccat ctacgccgtc accaaaaacg gcaaagcgcc caccgtctac gcgaccgact
     3301 gatgatcgaa aacgtctccc acgctgggga acagtgcccg tgcgcgccaa ttgaaaggct
     3361 tcgacggcaa tgacgaatgc ttcgtgtcca ttgagatacc gattcgccgc cggttttgct
     3421 gccgtcttgc ttctcgcggc gacgtcgcaa tctgcgggtg cgcaagcccc accaaagaac
     3481 ttcatcgtcc atgctgcgcc cacgccgatt gccgcgatcg gctttcagga tgatcagggg
     3541 cacgcaaaaa cgctggcgga tttcaagggc aaggtggtcg tcctcaatat ctgggcgacg
     3601 tggtgcacct catgccgcac cgaaatgccg gccctcgacc ggctggaggc ggatctaggc
     3661 ggcccaaaat tcgcagtcgt ggcgctgtcc atagatcggg gcggcatcag cagtatcgag
     3721 aagttctacg cggcgaccgg aataagccac ctgccgaaat acgttgatag ttcaggtcag
     3781 gtgctgcgcg gcctcggggc tcttgggctg ccgacgacac tccttcttga ccgcgaagga
     3841 aacgaagtcg gtcgcgtcgt cggtgcggtg gaatgggatg cggcggacac catcgcttat
     3901 ctcaagtccg tcattgcgga gcgatagcgg aggagaacgc catgacgcca tcaagccaaa
     3961 caacgaacaa ctcctttgcg cgcgatctgg taagtgtcgc gcaatactac cttcgtcccc
     4021 gatgggttct gttggggatc gcaatcgccg cgattgccat agggctcaca ttgaactgga
     4081 gctggctggt tgccgcgggg atcgcgccga tcctgctggc gatgttgccc tgcctcgtca
     4141 tgtgcggcct aggcctgtgc atgcacaaaa tggttggagc ttcc
//
LOCUS       DASSRL010000141         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1228709, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000141 DASSRL010000000
VERSION     DASSRL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228709"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..711
                     /locus_tag="VFL49_02965"
     CDS             187..711
                     /locus_tag="VFL49_02965"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7910965.1"
                     /translation="MTVAEFEKLPAPPGGYYELRHGEAVLVPPPQKLHNRVRRRLFLL
                     LEARLRSGGYVDVEVGFRPAPEHNILVADVAFVSAERWAAIGDHEWLSGSPELVIEVL
                     SPSNSAKEMNDKEQLCLGSEGQQFWAVDPDLRLVKVTTRDGLTRTYRGGATISLADFG
                     GETITVDSIFEAKS"
     gene            complement(729..1151)
                     /gene="atpC"
                     /locus_tag="VFL49_02970"
     CDS             complement(729..1151)
                     /gene="atpC"
                     /locus_tag="VFL49_02970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET7910966.1"
                     /translation="MADSFTIEIATPERSIANEEAVRAQIPGKDGYLGILPDHAPLLS
                     KLGIGTLSYVTPQGGKYNFAVHGGYVEVLNNHVRVLADEGEPGVEIDYQKAQREVEQR
                     SRELENLSAGSDPAQALSAVMSAQARAEAARKTAAGEE"
     gene            complement(1157..2617)
                     /gene="atpD"
                     /locus_tag="VFL49_02975"
     CDS             complement(1157..2617)
                     /gene="atpD"
                     /locus_tag="VFL49_02975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7910967.1"
                     /translation="MASTANTTQVVGHVIQIAGPAVDMQFPEGKIPIINNAIRVVSEG
                     FDVASPIDVVCEVAQHIGEGRVRTIALQPTDGMVRGMKAIDLGGPVSVPVGKQTLGRV
                     LNVIGEPVDNMGPIETNQRYPIHRHAPSFEEQATTQQMFETGIKVVDLLEPYLRGGKI
                     GLFGGAGVGKTVVIMELINNIAMKHGGVSVFAGVGERTREGNDLWLEMQESGVVDPHD
                     WHKSKCALIYGQMTEPPGARLRVGLTGLTVAEYFRDEEGQDVLLFIDNIFRFTQAGSE
                     VSALLGRMPSAVGYQPNLATEMGDLQERITSTKKGSITSVQAIYVPADDYTDPAPATA
                     FAHLDATTNLSRDIASLGIYPAVDPLASTSRILDPRILGDEHYGVAQQVKQTLQRYKD
                     LQDIIAILGIDELPEEDKLVVARARKIQRFLSQPFHVAEQFTGFKGKYVKLSDSIKGF
                     KELVDGKHDDIPEQAFWMQGTIEDVLERAEQMKKSI"
     gene            complement(2641..>3048)
                     /locus_tag="VFL49_02980"
     CDS             complement(2641..>3048)
                     /locus_tag="VFL49_02980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET7910968.1"
                     /translation="VSIAREVMQRFMDAEIDAVYLIGNEFKSMAAQIMTVTRLLPVDM
                     PATGERVSYIYEQPPSEILEELLPRYVEMSVYRAMLESSAAEHAARMTAMDAASSNAA
                     DMIDTLTLYLNRVRQASITKEIIEIVSGAAALG"
BASE COUNT          579 a          931 c          890 g          648 t
ORIGIN      
        1 tcgcaatgct tcgagtttgc gaccagaggt ttgaatttat cgaaaaatgc ggcggtttca
       61 ccgggatttt tgtacatctt ccagttgccc gccatcaggg gttttctggg cattatttat
      121 tcagaatatc gtgtggcctg acggtgtcgc gcgtacaatg aggttatgcc cgcgacgact
      181 gcattgatga cggtcgccga gtttgagaag ctgccggcgc cgcccggcgg ctactacgaa
      241 ctgcgccatg gagaggctgt actcgtgcca ccgccccaaa aactccacaa tcgagtacga
      301 cgacgattgt ttctgctgct cgaagcgcga ttacgctcgg gagggtatgt ggatgttgaa
      361 gttggatttc ggccggctcc agaacacaat atcctggtcg cagacgtggc gttcgtatcg
      421 gcagagcggt gggctgccat cggcgatcac gaatggcttt ctggctcgcc cgagttagtc
      481 attgaagtcc tttcgccctc gaattccgca aaggaaatga atgacaaaga gcaattgtgc
      541 cttggaagtg aaggccaaca gttctgggcc gtggatccgg acctcaggct ggtcaaggtc
      601 acgacgcgcg atggcctgac acgcacgtac cgcggcggcg cgacaatcag tctcgccgat
      661 ttcggcggcg agacgatcac agttgactca atcttcgaag ctaaaagctg acggctgccc
      721 gctgaaagct attcttcccc cgccgccgtc tttcgcgccg cttccgcgcg cgcctgcgcg
      781 ctcattacag ccgaaagcgc ttgcgccggg tcggagcccg ccgagagatt ctccaactcg
      841 cggctgcgct gctccacctc gcgctgcgcc ttctggtaat cgatttcgac gccgggctcg
      901 ccttcgtcgg cgagaactcg cacgtgattg ttcagcactt cgacatagcc gccgtgaacc
      961 gcaaagttgt acttgccgcc ttgtggggtg acatagctca acgttccgat acccagcttg
     1021 gaaaggagag gggcgtggtc cggcagaatg ccgagatagc catccttgcc cgggatctgc
     1081 gcccgaaccg cttcttcgtt cgcaatcgac cgttcaggcg tcgcgatttc gatggtgaaa
     1141 ctgtccgcca tggccgctaa atcgatttct tcatctgctc agcacgctcc agtacgtctt
     1201 cgatggtgcc ttgcatccag aaggcctgct ccggaatgtc gtcgtgcttg ccatctacca
     1261 gttccttgaa gcccttgatg ctatcggaga gtttgacata cttgcccttg aagccggtga
     1321 actgctccgc cacgtggaac ggctgcgaca gaaaccgctg gatcttgcgc gcgcgcgcaa
     1381 ccaccagctt gtcctcttcc gggagttcat cgatgcccag aatggcgatg atatcctgca
     1441 ggtccttata ccgctgcagc gtctgcttca cctgctgcgc aactccgtag tgctcgtcgc
     1501 cgaggatacg gggatcgaga atccgcgaag ttgaagcgag cggatcgaca gccggataaa
     1561 tgccgagcga tgcgatgtcg cgcgacaggt tggtggtggc gtccaggtgc gcgaaggcgg
     1621 tggccggcgc gggatcggtg taatcgtcgg ccggcacgta gatcgcctgc acggatgtga
     1681 tggaaccctt cttagttgac gtgattcgct cctgcaggtc gcccatctcg gtggcaaggt
     1741 tcggctgata gcccaccgcc gagggcatac gtccgagaag cgcggacact tcggacccgg
     1801 cctgcgtgaa gcggaaaata ttatcgataa acagcaggac gtcctggcct tcttcatcgc
     1861 ggaaatattc cgcaaccgtg aggcccgtga ggccgacacg caaacgcgcc ccgggcggct
     1921 ccgtcatctg gccgtaaatc agcgcgcact tgctcttgtg ccagtcgtgc ggatcgacga
     1981 cgccggactc ctgcatttcg agccacaggt cgttgccttc gcgagtgcgc tcacccacgc
     2041 ccgcgaacac ggaaactccg ccgtgcttca tggcgatgtt gttgatcaat tccatgatca
     2101 cgacggtttt cccgacgccg gccccgccga acagtccgat cttgccgccg cgcagatacg
     2161 gctccagcag atcgaccacc ttgatgccgg tctcgaacat ctgctgcgtt gtcgcctgct
     2221 cctcaaagga tggcgcgtgg cggtggatgg ggtagcgttg attcgtttcg atgggaccca
     2281 tgttgtccac cggctcaccg atgacgttca atacgcggcc cagcgtctgc ttgccgaccg
     2341 gcaccgacac cggtccgccg agatcgatgg ccttcatccc gcgcaccata ccgtcggtag
     2401 gctgcagcgc gatagtgcga acccggcctt cgccgatgtg ctgcgccacc tcgcaaacga
     2461 cgtcaatggg cgaggccaca tcgaagccct cgctcaccac gcggattgcg ttattaataa
     2521 tcgggatctt cccctcaggg aattgcatgt caacggccgg gccggcgatt tgaatgacat
     2581 ggcccaccac ctgggttgtg tttgctgtac tcgccatccg aaaactcctt ccgcgcccga
     2641 tcagcccaag gccgcggcgc cgcttacgat ctcgataatc tctttagtga tgctcgcctg
     2701 gcgcacgcga ttcaagtaca gcgtcagggt gtcgatcata tcggcagcgt tcgagctggc
     2761 cgcatccatg gcggtcatgc gcgccgcatg ctccgcagcc gacgattcga gcatcgcccg
     2821 gtacacggac atttcgacgt aacggggcag cagctcttcg agaatctccg agggcggctg
     2881 ctcgtaaatg tagctgacgc gctcaccggt ggccggcata tcgacgggaa gtaagcgcgt
     2941 gacggtcata atctgggcgg ccatgctctt gaactcgttg ccgatcaggt acacggcatc
     3001 gatttcggca tccataaacc gctgcatcac ctcgcgagca atgctgac
//
LOCUS       DASSRL010000142         8175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1088750, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000142 DASSRL010000000
VERSION     DASSRL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088750"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1928
                     /gene="fliF"
                     /locus_tag="VFL49_02985"
     CDS             216..1928
                     /gene="fliF"
                     /locus_tag="VFL49_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HET7910969.1"
                     /translation="MQGFFDFIKSLGAARMAAMAAVTLALIGFFSFLMVQMTTPQMVP
                     LFTDLSVDDSASIIKDLERQAVPYQLKSDGAIIMVPKDKVARLRMKLAESGLPKGGGV
                     GYEIFDKTDSLGATSFVQNINHLRALEGELARTIRSIDRVQAARVHLVLPERPLFSRD
                     KVEASASIVLKVRGALEPQQVRAIRHLVATAVNGLKPERVSVIDETGKLLADGATPDN
                     PLSGVTSDERKLAYENRLRGEVEAIVSSVVGPGHARVQINADFDVNRITQTSDKFDPD
                     GRVLRSSQTREEQSATTDNKEGTVSVGTELPGGNRQQQPAEGGSRGDQSRKTEEIVNY
                     EISRTTKTEVIEAGRVNRISAAVLVDGIYSKNDKGEVAYQPRPKEEIDRIAALVRSAI
                     GFDAKRGDQVEVVNLRFAESTPSPINEAAGWMSYLQFTKDDIMGAAEKGVMALLGLVV
                     LFFVVRPLVRRIITPEGAQAPFAAAPAVTIPAMTAEAAASAGMTLPGVNGNITSTGGP
                     NVSIVGGDEAVAISNRTSAMIDIAKVQGQVHAQSVQKVGELADKNPHEAVSIIRNWLH
                     EDAA"
     gene            1932..3035
                     /gene="fliG"
                     /locus_tag="VFL49_02990"
     CDS             1932..3035
                     /gene="fliG"
                     /locus_tag="VFL49_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HET7910970.1"
                     /translation="MPSAYAESKKDNKSTREDIGGLMAELSKRPHAPERTRKLTGPER
                     AAVLMLSLGEQYGAKIFSLLDDDELREISIVMSSLGTIEAEAVEQLLLEFVSRMSASG
                     ALLGNYDATERLLKQYLPPDRVGGIMDEIRGPAGRNMWEKLANVQEEVLANYLKNEYP
                     QTVAVVLSKLRPEHAARVLSILPEDLSLDCVNRMLKMEAVQKEVIERVEQTLRTEFMS
                     NLSQTRRRDAHEVMAEIFNNFDRQTETRFLTALEEDNRDAAERIKALMFTFDDLVKLD
                     TGSAQTLMRNIDKEKLAVALKGANETVRQFFFTNMSSRAAKLLVEDMQAMGPVRLRDV
                     DEAQALLVNVAKDLAAKGEIIIAKSRGEDELIY"
     gene            3100..3717
                     /locus_tag="VFL49_02995"
     CDS             3100..3717
                     /locus_tag="VFL49_02995"
                     /inference="COORDINATES: protein motif:HMM:NF004690.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HET7910971.1"
                     /translation="MKATAKYLFDEDFAAGEKPTITMVEHERRRADAESQAYRQGYTA
                     GQEQAHQEAIQRMADALSVIADGLGRLDGALAAIETRLETEAVEVAVAVAAKLSPALI
                     AREPFAEIASLATECFHHLVSTPLVTVRIAADIHEEAKGKIEEIARTRGYDGRLAILA
                     DDSLAPGDCRVEWADGGIIRDHTATASAIDETVARYIAARNTPAA"
     gene            3736..4098
                     /gene="fliN"
                     /locus_tag="VFL49_03000"
     CDS             3736..4098
                     /gene="fliN"
                     /locus_tag="VFL49_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HET7910972.1"
                     /translation="MSDEDTKVPLPDLENGSDVQVVQDAGTLAPSDSEPVDRGAADLE
                     AVFDVPVQVSAVLGRAHMEVGNLLKLGPGAVLELDRKVGEAIDIYVNNRLVARGEVVL
                     VEDKLGVTMTEIIKADHN"
     gene            4143..5516
                     /locus_tag="VFL49_03005"
     CDS             4143..5516
                     /locus_tag="VFL49_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7910973.1"
                     /translation="MRLLIVGTLGGQLTTATKLAMEKGASVTHAESNDQALAVLRSGR
                     GADLLMVDVSLNIRDLNARLDAEHIHVPIVACGVTNDARAAVNAIHAGAKEYIPLPPD
                     PDLIAAVLAAVADDTREMIYRDDAMAKMVKLAQQIAASDASVLITGESGTGKEVLARF
                     VHGRSNRSRKSFITVNCAAIPENLLESELFGHEKGAFTGALARRIGKFEEANGGTLLL
                     DEISEMDARLQAKLLRAIQERVIDRVGGNRPVPVDIRIIATSNRNLTDAVREGKFRED
                     LLFRLNVVNLKIPPLRERPADIGELANYFVRKYSQANGMPLRPLSAEARRALSTNRWP
                     GNVRELENTLHRAVLLSSGIEIGVDGILSPDGARLDQVRGTDVAAHAAMAAEQVTRNL
                     VGRTVAEVERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYAADGAPIPPPHGG
                     EPLKGAA"
     gene            5541..5960
                     /locus_tag="VFL49_03010"
     CDS             5541..5960
                     /locus_tag="VFL49_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HET7910974.1"
                     /translation="MNTDIGSLLFSFQGRINRAKYWLATVIYTAIVVVMIGAGYLFDF
                     SSLFFAVFVVVCIALFISGVAVGIKRLHDRDKSGWWLLLFYLVPGVFDGLDRVLGPNL
                     IFNLVSLAISLWMIVELGFLRGTSGPNRYGNDPLGVR"
     gene            5957..6301
                     /locus_tag="VFL49_03015"
     CDS             5957..6301
                     /locus_tag="VFL49_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HET7910975.1"
                     /translation="MTILYKICPAKLWREAERAGVFHGSEVDRRDNFIHFSTATQVVE
                     TAARHFASQNDLLLIRVDAGTLGSALKWEPSRGGALFPHLYARLDLAAVISVEPLPLG
                     PEGHHVFPPLKA"
     gene            6388..6945
                     /locus_tag="VFL49_03020"
     CDS             6388..6945
                     /locus_tag="VFL49_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7910976.1"
                     /translation="MDLDANRSLIAAALGRIPAGDRAALQTVYRLTSAKLFGVCLRIL
                     GEHGEAEDVLQEVYVTVWRKAADFDARRASPMTWLIAIARNRSIDRLRASRASRNMES
                     IDIAADVADTTPRADVALQGVQEHAKLHGCLDGLASHERAALRGAFFDGNTYEDLATR
                     MKVPLGTMKSWIRRAMFKLKACLEQ"
     gene            6942..7637
                     /locus_tag="VFL49_03025"
     CDS             6942..7637
                     /locus_tag="VFL49_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7910977.1"
                     /translation="MSDTFDHSGLPEDELLAAEYALGVLEGIERTTAEQRIARDRGFA
                     RQVTDWEERLAPWAAEIPEMLPPPHVWDRVATTLPPQIPRAGLWQNLAFWRGLSFAAG
                     ALAAACVAALIYFGAFTQTEPLVATIEGGGQRHFVATIDVRRGTVAVVPAAFAADATR
                     VPELWLIPPDGKPRPVGLLRADAPVTLTLSADLAGLAKNNAVLAVSLEPPGGSTTGLP
                     TGPVIATGKLTNL"
     gene            7724..>8175
                     /locus_tag="VFL49_03030"
     CDS             7724..>8175
                     /locus_tag="VFL49_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET7910978.1"
                     /translation="MKRISALALAGAAVFALSLGINATAPSFAAEKTVMVGGAAMYPS
                     RNIIQNAVNSKDHTTLVAAVKAAGLVETLSGDGPFTVFAPTNAAFGKLPAGTVESLLK
                     PENKAQLTKILTCHVVSANAMSSAIKKMIKDDAGMHPVKTVGGCTFTA"
BASE COUNT         1616 a         2615 c         2528 g         1416 t
ORIGIN      
        1 ggttgcgcac gacacgcatc cagcagtacc gccagtaacg ctctcagcgt tcggtctgca
       61 cgccggcgaa gtacgccggc gtttttattt gttcggcgcc actcgcccaa cgactttcgg
      121 cagtccacca gccggaaatt aaccggacgc taaccttaaa ctgggcaaat cttgccgggt
      181 gcggcgcgcg gcgcgcctgc gggtaggaca aaccggtgca aggcttcttc gacttcatca
      241 agtcgctcgg tgctgcgcga atggcggcga tggccgcggt aacgctcgcc ctgatcggct
      301 tcttctcgtt cctgatggtt cagatgacga cgccgcaaat ggtgccgttg ttcaccgatc
      361 tttcggtcga cgacagtgcg tcgatcatca aagacctgga gcggcaggcc gttccatatc
      421 aactcaagag cgacggcgcg atcatcatgg tcccgaagga caaggtcgcc cgcctgcgca
      481 tgaagctcgc ggaatccggc ctgccgaaag gcggcggcgt cggttacgag atcttcgaca
      541 agacggactc tctcggtgcg acgagtttcg tccagaacat caaccacctg cgggcactcg
      601 aaggcgaact tgcccgcacg atccgcagca tcgatcgtgt gcaggcggcg cgggtgcacc
      661 tcgtgcttcc ggaacgtccg ctcttctcgc gcgacaaggt cgaggcttcc gcctcgatcg
      721 tgctcaaggt gcgtggcgcg ctcgagccgc agcaggtgcg ggccatccgc cacctggtcg
      781 caactgcagt caacggcctg aaacctgagc gcgtttccgt catcgacgaa accggcaaat
      841 tgcttgccga cggggcgacg ccggacaacc cgctcagcgg ggttacctcc gacgagcgca
      901 agctcgccta cgagaaccgg cttcgcggcg aagtcgaggc gatcgtttcc tcggttgtcg
      961 gcccgggcca tgcacgcgtg cagatcaatg ccgattttga cgtgaatcgg atcacgcaga
     1021 catcagacaa attcgacccg gatgggcggg tgttgcgctc gagccagacc cgcgaagagc
     1081 agtccgccac gaccgataac aaggaaggca cggtgtccgt cggcaccgaa ctgcccggcg
     1141 gcaacaggca gcagcaaccc gccgaaggcg gctcccgcgg cgaccaaagc cgcaagaccg
     1201 aggaaatcgt caactacgag atctcccgca ccacgaagac cgaggttatc gaggcagggc
     1261 gcgtgaatcg tatctcggca gccgtgctgg tcgacgggat ctacagcaag aacgacaaag
     1321 gcgaggtcgc ctaccaaccg cgcccgaaag aagagatcga ccggatcgcc gccctcgtgc
     1381 gctccgcgat cggtttcgac gccaagcgcg gcgaccaggt ggaggtggtg aacctgcgtt
     1441 ttgccgagtc gacgccgtca ccgatcaacg aagcggccgg ttggatgagc tatctgcagt
     1501 tcaccaaaga cgacatcatg ggcgccgccg aaaagggtgt gatggcgctg cttgggctgg
     1561 tcgtcctgtt cttcgtggtt cggccgctgg tccgccggat catcacgccg gagggggcgc
     1621 aggcgccgtt cgccgccgca ccggccgtca ccattcccgc aatgacagcc gaagcagcgg
     1681 cgtccgccgg catgacgcta ccgggcgtga acggcaacat cacctcgacc ggaggcccga
     1741 acgtctcgat cgtcggcggc gacgaagcgg tcgcaatttc caaccgcacg tccgcgatga
     1801 tcgatatcgc caaggtacag ggccaggttc acgcccagtc cgtgcaaaaa gtcggcgagc
     1861 tcgccgacaa gaacccgcac gaagccgtgt cgataatccg caactggctc catgaggacg
     1921 cagcctaagt catgccgtca gcctacgccg aaagcaaaaa agacaacaag tcgacccgcg
     1981 aggacatcgg ggggttgatg gccgaactct caaagcgccc gcacgccccc gaacgcaccc
     2041 gcaagctcac cggtcccgag cgcgccgcgg ttctcatgct ctcgctcggc gagcagtacg
     2101 gcgccaagat cttcagtctg ctcgacgacg acgagttgcg cgaaatttcc atcgtgatgt
     2161 cgtcgctggg caccatcgaa gccgaggcgg tcgagcaatt gctgctcgaa ttcgtctcgc
     2221 gcatgtcggc gtcaggcgcc ctcctcggca actacgatgc caccgagcgc ctgctcaagc
     2281 agtatctgcc gccagaccgg gtcggcggca tcatggacga gatccgtggt cccgccgggc
     2341 gcaacatgtg ggagaagctt gccaacgtcc aggaagaggt gctggcgaac tatctcaaga
     2401 atgagtatcc gcagaccgtc gccgtcgtgc tgtcgaagct gcggccggaa cacgccgcgc
     2461 gggtcctttc catcctgccc gaggaccttt cgctcgactg cgtcaatcgc atgctgaaaa
     2521 tggaagccgt gcagaaggaa gtgattgagc gcgtcgagca gactctgcgc acggaattca
     2581 tgtccaacct gtcgcaaacg cggcgtcgtg acgcgcacga ggtgatggcg gaaatcttca
     2641 acaatttcga ccggcagacg gaaacccgct tcttgacggc actcgaggaa gacaatcgcg
     2701 acgccgccga gcgcatcaag gcgctgatgt tcaccttcga cgatctggtg aagctcgaca
     2761 ccggctcggc gcagacactg atgcgcaata tcgacaagga aaagctggcg gtcgcgctca
     2821 agggcgcgaa cgagacggtg cgacagttct tcttcaccaa catgtcgagc cgtgcggcca
     2881 agctgcttgt ggaagatatg caggcaatgg gaccggttcg cctgcgtgac gtcgacgaag
     2941 cgcaggccct ccttgtcaac gtcgccaagg atctcgcggc caagggcgaa atcatcatcg
     3001 ccaagagccg cggggaagac gaactgatct attgaggcgc gcatgatccg gaaaagcggg
     3061 aaccggtttt ctgaaaagat catgcgcaaa gggaagtgaa tgaaagcgac cgccaaatat
     3121 ctgttcgacg aggatttcgc cgccggcgag aagccgacca ttaccatggt cgagcatgag
     3181 cgcaggcgcg ccgatgccga atcgcaggct tatcggcagg gctacactgc agggcaggag
     3241 caggcgcatc aggaagccat tcagcggatg gccgacgcgc tctcggtgat cgccgacggg
     3301 cttggccgtc tcgatggggc gcttgccgca atcgagaccc gtcttgaaac ggaggctgtc
     3361 gaggtggcgg tcgcggtggc cgccaagctg tcgccggcgc tgatcgcgcg cgagccgttc
     3421 gccgagatcg catcgctcgc gaccgaatgc ttccaccatc tggtgtccac gccgcttgtc
     3481 acagttcgca ttgcggcaga catccatgag gaggcgaagg ggaagatcga ggagatcgcg
     3541 cgaacgcgcg gctacgacgg ccgcctggcc atccttgcag acgacagcct cgcacccggt
     3601 gactgtcgcg tcgaatgggc cgacggcggc atcatccgtg accataccgc aacggcatcg
     3661 gcgatcgatg aaacagtcgc ccgctacatc gcggcccgca acacgcctgc cgcataagat
     3721 ccggagacga cggcaatgag tgacgaagac accaaggtac cgcttccaga tctcgagaac
     3781 ggcagcgacg tgcaggtcgt gcaggatgcc ggcacgctgg caccctccga cagcgagccc
     3841 gtcgatcgag gggcggcaga tctcgaagcg gtgttcgacg ttccggttca ggtttcagcg
     3901 gtgctgggac gggcgcacat ggaagtcggc aatttgctca agcttggccc tggcgcggtg
     3961 ctcgagctcg accgcaaggt cggcgaagcg atcgacatct acgtcaacaa ccggctggtt
     4021 gcccgcggcg aagtggttct tgtcgaggac aagctgggag tgaccatgac ggaaatcatc
     4081 aaggcggatc ataactgatg caacaaggat ccgcccttac tcgaatgaca cggagctaag
     4141 ccatgcgact tctcattgtc ggaacgctcg ggggccagct caccaccgca accaagctcg
     4201 caatggaaaa aggcgcgagc gtcacccacg ccgaaagcaa cgaccaggcg ctggcggtgc
     4261 tgcgctccgg ccgcggagcc gatcttttga tggtggatgt cagcctcaac atccgcgatc
     4321 tcaacgcgcg tctcgatgcc gagcatatcc atgttccgat cgtcgcctgt ggcgtcacca
     4381 atgacgcgcg cgcagccgtc aatgcgattc atgccggcgc gaaagaatac atcccgctgc
     4441 cgccagatcc cgatctgatc gcagccgtgc tcgccgccgt tgccgatgat acgcgtgaga
     4501 tgatctaccg cgacgacgcc atggcgaaaa tggtcaagct cgcgcagcag atcgcggcgt
     4561 ccgacgcttc ggtccttatc actggtgaaa gcggaaccgg aaaggaagtg ctggcgcgct
     4621 tcgtgcacgg ccgcagcaac cgctccagga agtcgttcat caccgtcaac tgcgcggcga
     4681 tcccggaaaa cctgcttgaa tcggaattgt tcggtcacga gaagggcgcc ttcactggcg
     4741 ccctggcgcg ccgtatcggc aaattcgagg aagccaacgg cggcacgctc ctgcttgacg
     4801 aaatctccga gatggacgcc cgcttgcagg cgaagctctt gcgcgcaatt caggaacggg
     4861 tcatcgaccg cgtcggcggc aaccgcccgg tgccggtcga cattcgcatc atcgccacgt
     4921 ccaaccgcaa tctcaccgac gccgtacgcg aagggaaatt ccgtgaggac ctgctgttcc
     4981 gcctcaacgt cgtgaacctg aagattccgc cactgcgcga acgtccggcc gatattggcg
     5041 aactggcgaa ctacttcgtg cgtaagtatt cgcaagccaa cgggatgccg ctgcggccgc
     5101 tgtccgccga ggcgcgccgc gccctcagta cgaaccgctg gccaggcaac gtacgcgagc
     5161 tcgagaatac gctgcatcgc gccgtgctgc tttcgagcgg catcgagatc ggcgtcgacg
     5221 gcattctttc acccgacggc gcgcggctcg atcaggtgcg tggcacggac gtcgccgccc
     5281 atgcggcgat ggccgccgag caggtcaccc gcaatctggt cgggcgtacc gtggccgaag
     5341 tcgagcgcga tctgatcctg gaaacgctca agcattgcct cggcaaccgc acgcacgccg
     5401 ccaatattct cggcatctcg atccgcacgt tgcgtaacaa gctcaatgag tatgccgccg
     5461 acggcgcgcc aattccgccg ccccacggcg gcgagccgct caagggggct gcataggcag
     5521 gcaacgcaac cggggcgaca atgaacacgg atatcggttc gctgctgttt tcttttcagg
     5581 ggcgcatcaa tcgcgcaaaa tactggctcg cgaccgtcat ttacaccgct atcgtggttg
     5641 tgatgatcgg tgcgggatat ctgttcgatt tcagcagcct gttctttgcc gtttttgtgg
     5701 tcgtctgcat tgccctgttc atttccggtg tcgccgtcgg catcaagcgc ctgcacgacc
     5761 gcgacaagag cggctggtgg ctattgctgt tctacctggt gcctggggtc ttcgacggcc
     5821 tggacagggt gctgggcccg aatttgatct tcaatctcgt gtcgcttgca atatcgctct
     5881 ggatgatcgt cgagctgggc ttcctgcgcg gcacatccgg gccgaaccgg tatggtaacg
     5941 acccactcgg cgttcggtga cgatccttta caaaatttgc cccgccaaac tgtggcggga
     6001 agccgagcgg gccggtgtat tccacggatc cgaggtggat cgccgcgaca acttcattca
     6061 tttttcgacc gcaacacagg tcgtggaaac cgccgccagg cattttgcca gtcaaaacga
     6121 tctgctcctg atcagggtgg atgccgggac gcttggcagc gccctgaagt gggagccgtc
     6181 gcgcggcggc gcgctgtttc ctcaccttta cgctcgtctc gatctcgccg ccgtcatcag
     6241 cgtcgaacca ttgccgcttg gccccgaggg gcatcacgtt tttcccccgt taaaggccta
     6301 attcgttcag ctgtcgcggc cgtaattgtg ggcagaccgt caaaacctta aggtggcccg
     6361 cctgccaaaa tccgggcccg cattccgatg gaccttgatg ccaaccgcag cctgatcgca
     6421 gccgcgctcg gccggattcc ggctggcgac cgggcggccc tgcaaacggt ctatcgcctc
     6481 acctccgcga agctttttgg tgtctgcctg cgtatcttgg gtgagcacgg cgaggcggag
     6541 gacgtgctgc aggaggtcta cgtgacggtg tggcgcaagg ccgccgattt cgacgccagg
     6601 cgggcgagcc cgatgacctg gctgatcgcc atcgcccgca atcgctcgat cgaccggctg
     6661 cgcgcctcgc gcgcaagccg caacatggag tcgatcgaca tcgctgccga cgttgccgac
     6721 accacacctc gcgccgacgt ggcgctgcaa ggtgtccagg agcacgcaaa attgcacggt
     6781 tgtctcgacg gcctggcgtc gcacgagcgt gcggcgctgc gcggcgcgtt cttcgacggc
     6841 aatacctacg aggatcttgc gacgcgcatg aaggtgccgc tcggcaccat gaagagctgg
     6901 atccgccgcg ccatgttcaa attaaaggca tgccttgagc aatgagcgac acgttcgacc
     6961 atagcggctt gcccgaagac gaattgctcg cagccgaata tgcgctgggc gtgcttgagg
     7021 gcatcgagcg caccacggcc gaacagcgca ttgcgcgcga ccgcggcttt gcgcgccagg
     7081 tcacggactg ggaggaacgc ctcgcgccct gggccgcaga gattccggaa atgctgccac
     7141 cgccgcacgt ctgggaccgc gtggccacca ccctgccccc gcaaattcca cgcgccggtt
     7201 tgtggcagaa tctcgcgttc tggcgtggac tgagcttcgc agccggcgcc ctcgccgcgg
     7261 cctgcgtcgc agcgctgatc tattttggag cattcacaca aacagagcct cttgtcgcaa
     7321 cgatcgaagg cggcggccag cggcatttcg tggccaccat cgatgtaagg cgcggcaccg
     7381 ttgcggtcgt tcccgctgcc ttcgccgccg acgcgacgcg tgtccccgag ctgtggctca
     7441 tcccgccaga cggcaagcca cgaccggttg gcctgttgcg cgcggatgcg ccggtcacgc
     7501 tgacgctttc cgccgatctt gccggcctgg cgaaaaacaa cgcggtgctg gccgtttcac
     7561 tcgagccgcc gggcggatcg accaccggct tgccgaccgg tccggtgatc gccaccggca
     7621 aactcacgaa cctgtgaccg cgctcctgca tctgtaattt caaggcctcc gtagctctca
     7681 cgtctcccct taggggacag caaacagagc aacggagaga accatgaaac gtatttctgc
     7741 actcgcactc gctggcgccg ccgtattcgc gctcagcctc ggaatcaacg caaccgcccc
     7801 gagcttcgcc gccgagaaaa ccgtaatggt cggcggcgca gcgatgtacc cgtccaggaa
     7861 catcatccag aacgcggtga attcgaagga ccacaccacc ctggtggcgg ccgtgaaggc
     7921 tgcggggctg gtcgagacgc tttccggcga cggcccgttc acggtgttcg caccgaccaa
     7981 tgccgccttc ggcaagttgc cggcgggcac ggtcgagagc ctgctcaagc cggagaataa
     8041 ggcgcagctc acgaagatcc tgacctgcca tgtcgtgtcg gcaaacgcga tgtcctcggc
     8101 gatcaagaag atgatcaagg acgatgccgg catgcaccct gtgaagaccg tcggcggctg
     8161 tacgttcacc gccaa
//
LOCUS       DASSRL010000143         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_913899, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000143 DASSRL010000000
VERSION     DASSRL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913899"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1605)
                     /locus_tag="VFL49_03035"
     CDS             complement(163..1605)
                     /locus_tag="VFL49_03035"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7910979.1"
                     /translation="MRLPLILAATMALASTAAPAADLLASRTSAASKQARAGEAEFRD
                     LYRELVEINTTASEGSCTVAANAMKARLLTAGFPEKDLHVLVPPQRPTDGNLVAVLPG
                     TDAKLKPLMLLAHIDVVEAKAADWERDPFKLTEEDGYFYARGASDDKAMAAIFTDAMV
                     RFQHEGYLPRRGIKLVLTCGEETPKRFNGVTWLIEHERELIDAEFALNEGGGGRLDGK
                     TGQYLYNGILAGEKLYQDFTFTTTNQGGHSSRPTPDNAIYQMSQALAKVQAFEFPIEF
                     NPTTRAYLAKFADVVGGTQGADMKAAATSADPAAIERLKQDPNINGMLHTTCVATLIN
                     GGHAPNALPQHVSANVNCRIFPGHAPEEIRQTLIRIVNDPGVKIEFEAPPEKGGPPPP
                     LTAQILGPIEKLSADMFPGVPLIPTQASGATDGRFLTPAGIPTYGVSGIFSDGATTNA
                     HGLNERIRVQSLLEGREFLYRLTKLYAGGK"
     gene            1873..>2109
                     /locus_tag="VFL49_03040"
     CDS             1873..>2109
                     /locus_tag="VFL49_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910980.1"
                     /translation="MIRSSTGVLVLTALLAAATASTATAKPAAESGKASVARIDKTRI
                     DATLGDFVKSNQLVGVSALVFQDGQEAYFGAFGLA"
BASE COUNT          359 a          620 c          694 g          436 t
ORIGIN      
        1 ggttggcggt tggcggttgg cggttgagag aagaggtgaa gattgtcttt tcactccttc
       61 ccccgcttgc gggggaaggt tgggatgggg gcattcgagc actcgccttc aacgcacagc
      121 ttcagctgtt tgcgctggcg cggtggctgc cctcctgatc tctcatttgc cgccggcata
      181 cagtttcgtg agccggtaca ggaactcgcg gccttccagc agcgattgca cgcggatgcg
      241 ttcgttgagg ccgtgagcat tcgtggtggc accgtcggag aaaatgccag agacgccgta
      301 ggtggggatg cctgcgggag tgagaaagcg tccatcggtt gcgcccgatg cctgcgtcgg
      361 aatcagcggc acgcccggga acatgtccgc actcagtttc tcgatgggac cgaggatctg
      421 cgcagtgagc ggcggcggcg gtccgccctt ttccggcggc gcctcgaatt caatcttcac
      481 accggggtca ttcacgatac ggatcagcgt ctggcggatc tcttccggtg catggcctgg
      541 gaagatgcgg cagttgacgt tcgccgatac gtgctgcggc aatgcgttcg gcgcgtgacc
      601 gccgttgatc agcgtcgcca cgcacgtcgt gtgcagcatg ccgttgatgt tcgggtcctg
      661 tttcagccgc tcgatggctg cggggtcagc ggatgtcgcc gcagccttca tgtccgcgcc
      721 ctgcgtgccg cccaccacgt ccgcgaactt cgcaagatag gcgcgcgtgg tcggattgaa
      781 ctcaatcgga aattcgaacg cctgaacttt cgccagcgcc tgcgacatct gatagatcgc
      841 gttgtcagga gtcggccggg acgaatgacc gccctggttg gtcgtggtga acgtgaaatc
      901 ctggtagagc ttttcgccgg ccagaatgcc gttgtagaga tactggccgg tttttccgtc
      961 gagccgcccg ccgccgcctt cattcaatgc aaattcggcg tcgatcagct cgcgttcgtg
     1021 ttcgatcagc cacgtgacgc cgttgaagcg cttcggtgtc tcctcgccgc aagtgagtac
     1081 gagcttgatg ccgcgcctgg gcaggtagcc ttcatgctga aagcgcacca tggcgtccgt
     1141 gaagatcgcg gccatggcct tgtcgtccga agcgccacgt gcgtaaaagt agccgtcctc
     1201 ttcggtcagc ttgaaaggat cgcgttccca gtccgcggct ttggcctcaa ccacatcgat
     1261 gtgggccaac agcatcagcg gcttgagctt cgcatccgtg cccggcagga ccgccaccag
     1321 attgccgtcg gtgggtcgct gcggcggcac gagcacatgc aggtcctttt cgggaaatcc
     1381 cgctgtcagc aagcgggctt tcatggcgtt ggctgccacg gtgcacgagc cttccgacgc
     1441 ggtggtgttg atctccacca gttcgcggta gagatcgcgg aattcggctt cgcccgcgcg
     1501 cgcctgcttc gaagcggctg aggttcggct ggcgagcagg tcggcggccg gagcggcagt
     1561 tgacgccaat gccatggtgg cggcgaggat caatgggagg cgcatgaatc acctgcgatg
     1621 atggcgggtg cggcagcata gtcgcgcagg ccctgtcatc tccagccggg ttgtccggtc
     1681 ttctcacgac gtgattgggc aaaggcctga gtcgtcaccg gtgccgacgc agattacaaa
     1741 tgcgcggacg aggccggcag ccgtgttcat ggccagaagc acttgccata ctgcccgatc
     1801 acgaaacgca gcgaacgcgc ggtgggcgtg cgatttcgcc agaacaatct tcagagaaga
     1861 aagggggaat ccatgatccg ttcgtccaca ggtgttcttg ttctgacggc actgctcgca
     1921 gctgccacgg cctccaccgc gacggcgaaa cctgcggccg agtccggcaa ggccagcgtc
     1981 gcacgcatcg acaagacacg catcgatgcg acgctcggcg attttgtgaa atcgaatcag
     2041 ctggtcggtg tgtcggcgct ggtgtttcag gacggacagg aagcgtattt cggtgcgttc
     2101 ggccttgcc
//
LOCUS       DASSRL010000144         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1368737, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000144 DASSRL010000000
VERSION     DASSRL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368737"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFL49_03045"
     CDS             complement(<1..609)
                     /locus_tag="VFL49_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET7910981.1"
                     /translation="MFGKRSATTEARVAVSPAPVAPAPAPLPFTESPQTAPAVPVETG
                     RTPFPSDGLGAPIVSAPAAPRPPANPAPTAVDTRRSEAYYETKTTIFGALIEAIDLAQ
                     LARLDVDSAREEIRDIVNEIIAIKNIVMSISEQEDLLDDICNDVLGFGPLEPLLARDD
                     IADIMVNGSGTVYIEVHGKIQRTGIRFRDNQQLLNICQRIVSQ"
     gene            complement(631..1923)
                     /locus_tag="VFL49_03050"
     CDS             complement(631..1923)
                     /locus_tag="VFL49_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7910982.1"
                     /translation="MIKHAQQAAVADNASTPPAASDAAADAHIAPAPRISVQAFCETV
                     ESAAAVQAAGEDRRLAKAHVKIQMGGIGAAIETFRSSPTPNVILIETDAHATDILGGL
                     DQLAECCDAGTRVIVMGRVNDVTLYRELTRRGVSEYMITPINTIDVVRAVCHLFSAPD
                     AKPVGRIVAVVGAKGGVGASTVAHNVAWAIGRDLSIESVVADLDLPFGTAGLDYNQDP
                     PQGIADAVFSPDRVDTAFVDRLLSKCTDHLNLLAAPATLERVYDFGAEAFDSILDSLR
                     ATMPCIVLDVPHQWAGWTKRLLVSADEILVVAGPDLANLRNAKNLIDLLRASRPNDHH
                     PRYCLNQVGIPKRPEISPADFAKALEASPLAVIPFEPQLFGPAANNGQMIGEMSASHK
                     TAEIFRQIAQALTGRGEVKKARRGLLPPLLSKIVKRKG"
     gene            complement(1920..>2616)
                     /locus_tag="VFL49_03055"
     CDS             complement(1920..>2616)
                     /locus_tag="VFL49_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HET7910983.1"
                     /translation="AVLRALMAGCVAVTVAGCYQTEVAQKEYPTDYRQRHPIVVREGS
                     QNIDILLGRNRGGLTASQRADVVSFAQVWRREAGSGIIIETPKGGPTDHAASEATREI
                     RSIFTAVGVPSNAIYVRSYRPPKTSLASIRLNYTKLIAEAGPCGEWPKDLGPSWDSGF
                     TSNQPYWNFGCAMQRNLAAMVDNPADLVQPRGEIPAYAPRRSVVLDKWRKGENPSGSY
                     TGYDTGKISDLGK"
BASE COUNT          493 a          768 c          835 g          520 t
ORIGIN      
        1 ctggctgacg atgcgctggc agatgttgag gagctgctgg ttgtcgcgga agcggatgcc
       61 ggtgcgctga atcttgccat ggacttcgat gtaaaccgtg ccggaaccat tcaccatgat
      121 gtcggctatg tcgtcacgcg caagcagcgg ttcgagcgga ccgaagccaa ggacgtcatt
      181 gcagatatcg tcgagcaggt cttcctgctc cgagatcgac atcacgatgt tcttgatcgc
      241 gatgatctca ttgacgatat cgcggatttc ctcgcgcgcg gaatcaacat caagccgggc
      301 cagctgcgcg aggtcgatcg cctcgatgag cgcgccgaaa atggtggttt tggtctcgta
      361 atacgcttcc gatcgacgcg tatcgacggc ggtcggggcg ggattcgcag gcgggcgcgg
      421 cgcagccggc gccgagacga tcggcgcacc cagcccgtca gacggaaacg gcgtgcgccc
      481 ggtttcaaca gggaccgccg gagcggtttg tggagactcg gtaaacggaa gcggcgccgg
      541 tgccggcgct accggcgcgg gcgatacggc gacacgagct tctgtagttg cgcttcgctt
      601 accgaacaag acgagttact cccgacaatt tcagcctttg cgcttgacga tcttcgaaag
      661 caaaggcggc agcagaccac ggcgggcctt tttgacttca ccgcgcccgg taagcgcctg
      721 ggcgatctgt cgaaagattt ccgcggtctt gtgacttgcc gacatttcac cgatcatttg
      781 accgttgttg gcggccggcc cgaacagctg tggttcgaac ggaatgacag ccagcggact
      841 ggcttccagc gcctttgcaa agtcagccgg cgagatttcc gggcgtttcg gtatgcctac
      901 ctggttgagg cagtagcgtg gatgatgatc gttcggccgc gacgcacgca gcagatcgat
      961 gagattcttg gcattgcgga ggttggccag atccgggccg gcaacgacaa gaatttcatc
     1021 cgcactgacg agaaggcgct tggtccagcc ggcccattga tggggaacgt cgagaacgat
     1081 gcacggcatg gtcgcgcgca gcgaatcaag tatcgaatcg aaggcttcgg cgccgaagtc
     1141 gtagacccgc tcaagggttg cgggcgccgc cagcagattg agatggtcgg tgcatttcga
     1201 cagcagacgg tcgacaaatg ccgtgtcgac ccgatcggga gagaagacgg cgtcagcgat
     1261 gccctgaggc ggatcctgat tgtagtcgag accggcggtg ccgaagggca gatcgagatc
     1321 cgcaacaacg gattcaatcg agagatcgcg accgatcgcc caggcaacgt tgtgagccac
     1381 cgtggacgcg cctacgccgc ctttggcgcc tacaaccgca acgatacggc ctacgggctt
     1441 ggcgtcgggt gccgaaaaca ggtggcagac tgcccgaacc acatcgatgg tgttgatcgg
     1501 cgtgatcata tattcgctca cgccgcgccg tgtcagttcc cgataaagcg tgacgtcgtt
     1561 gacgcgaccc atcacgatca cgcgtgttcc ggcgtcacag cactccgcca gctgatcgag
     1621 tccgccaaga atgtccgtag cgtgcgcatc cgtttcaatc agaatgacgt tcggcgtagg
     1681 cgagctgcgg aaagtctcga ttgctgcacc gataccgccc atctggattt tgacgtgagc
     1741 ctttgcgagg cgccgatctt cacctgcggc ctggaccgcg gccgcgcttt caacggtctc
     1801 gcaaaaggcc tgcaccgaaa tgcgcggcgc cggcgctatg tgcgcgtcgg ccgcggcgtc
     1861 tgaggcggcg ggcggcgttg aagcgttgtc tgcgactgcg gcttgctgag cgtgctttat
     1921 catttgccta ggtcactgat tttgccggtg tcataaccgg tgtaactgcc ggaggggttc
     1981 tcgcccttgc gccatttgtc gagcacgacc gaccggcgcg gtgcgtatgc cgggatctct
     2041 ccgcgcggct gcaccaggtc ggccggatta tcgaccatgg ccgcaaggtt gcgctgcatg
     2101 gcacagccga aattccagta cggctgatta ctggtaaatc ccgaatccca ggagggtccc
     2161 agatcctttg gccattctcc gcagggaccc gcctccgcaa tcagcttcgt atagttgagt
     2221 ctgatgcttg caagcgaggt cttcgggggc cggtaactgc gcacatagat cgcgttcgac
     2281 ggcacgccga ccgcggtaaa gatcgaccgg atctctcgtg ttgcctctga ggccgcatgg
     2341 tcggtggggc caccctttgg agtctcgata atgattccgc tgcctgcctc gcggcgccac
     2401 acttgggcaa aagagacgac atcggcgcgc tgactcgccg tcagcccccc acggttgcgg
     2461 ccaagcagga tatcgatgtt ttgtgagcct tcacgcacca cgatcgggtg gcgttggcga
     2521 tagtccgtcg gatattcttt ttgcgcgact tcggtctgat agcagccggc caccgtgacg
     2581 gcgacgcagc ctgccatcag tgcgcgaagc accgct
//
LOCUS       DASSRL010000145         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_983731, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000145 DASSRL010000000
VERSION     DASSRL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983731"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFL49_03060"
     CDS             complement(<1..410)
                     /locus_tag="VFL49_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910984.1"
                     /translation="MKAGTHRCAFLIVTSLLVSVPQVHAQAISPAVLAEYQAKLAQYQ
                     AARGAYDAEADAYWDAVTAKRRVRNAKRREHSRIELDDYVLTQPPIYSGPPRPVDPRT
                     PAQPPPERPDIPVVADFLQNAAEQFGFVPQRPAS"
     gene            complement(510..719)
                     /locus_tag="VFL49_03065"
     CDS             complement(510..719)
                     /locus_tag="VFL49_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910985.1"
                     /translation="MNWSMYTADRSTHLKIVVVGLAAALLIAVIGISARELNLGVDIM
                     TAQNPSVIKAGGPVVFTDRTAPTVR"
     gene            complement(923..>2649)
                     /locus_tag="VFL49_03070"
     CDS             complement(923..>2649)
                     /locus_tag="VFL49_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HET7910986.1"
                     /translation="FVIGIIVFTILIIVNFVVITKGSGRIAEVAARFHLDAMPGKQMA
                     IDADLSAGLIDEKEARRRRQKLEDEGGFFGAMDGASKFVRGDAIAGLLVVFINVIGGM
                     IIGIAQQGLSFSDAAHSYTILTVGDGLVTQVPALIVSTAAGLLVSKASVAGAADKALL
                     SQLSGYPKALGMSGAVMLVMSLLPGIPLIPFMALGSGAAALAFIVDRRMKLTLATEAQ
                     KTEAAAKVEAADEPIAAALKIDDLKVELGYALLPLVNAPDGSDRLTEQIKALRRSLAV
                     EMGFVMPAVRLLDNVQLDANTYVIKIKEVEAGSGKVWAGQYMVMDPAGGQVKLPGTHT
                     TEPTFGLPATWIDASHKEDASMKGYTVVDAATVLSTHLTELLKSNMAELLSYGEVQKL
                     LKELPKEQADLVKDLVPNLITVSGIQRVLQILLAERVSVRDLATILEGIADGIAGSRN
                     PVMLAEHVRARLSRQLCAQHTSPGGYLPLVALSAKWEQAFAESMVGQGDDRQLAMQPS
                     RLSEFITLVRERFEEAAREGEAPVLVTSANIRPYVRGIVERFRAQTPVLSQSEIHPRS
                     RLKTVGSI"
BASE COUNT          493 a          793 c          858 g          505 t
ORIGIN      
        1 tcgctcgcag gccgctgcgg aacgaaaccg aactgctcgg cagcgttctg caaaaagtcg
       61 gcgacaaccg gaatatcggg acgctccggc ggcggttgcg ccggcgtccg cgggtcgacg
      121 gggcgcggtg gtcctgaata gatcgggggc tgcgtgagca catagtcgtc aagctcaatg
      181 cggctgtgct cacggcgctt cgcgttgcga acgcgacgtt ttgccgtgac agcatcccaa
      241 taggcatcgg cctcggcgtc gtaggcgccg cgggcagcct gatattgcgc aagctttgcc
      301 tgatactcag cgagcacagc aggcgagatg gcttgcgcat gcacctgcgg cacagaaaca
      361 agcaagctcg tcacgatcag gaatgcacag cgatgcgtac cggctttcat gcgcaatgtt
      421 gtcggccatt tttctcgatt cggccacttc acaacaaaac gcgccgcatt tgcggcgcgt
      481 cggattttgc ggcgcgagcg atcgaagcgc taacggacgg ttggtgcggt tcggtcagtg
      541 aatacaaccg gtccgccggc tttgatcacc gacggattct gtgcggtcat aatatctacg
      601 ccgaggttga gttcgcgcgc cgagatgccg attacggcaa tcaacagggc tgcggcaagt
      661 ccgaccacca cgatcttgag atgggtgctg cggtcggccg tgtacatgga ccagttcatg
      721 gcgttctccg gggtccctgc tcctttgtct ccccgtcctg acaaaaggtt caactggatc
      781 gagccattaa cgtaagtaag ccgccacagt tcccaaggac ccatcacgga tgattgaatc
      841 cctgtgagcg cgggcgattt gcctgcgggt acggcatctt agcggccgcc agcggcgttt
      901 cgacgcgatt ccggcggagc ggctagatgc tcccgacggt cttcaggcgc gagcggggat
      961 gaatctcgct ttgcgagaga accggtgtct gcgcgcggaa ccgttcgacg attcctcgca
     1021 cgtaggggcg gatgttcgcc gatgtgacca gcacgggtgc ttcaccttcg cgggcagctt
     1081 cctcgaagcg ctcgcggacc agagtgatga attcggacag gcgcgagggc tgcatggcaa
     1141 gttgccggtc gtcgccctgg ccgaccatcg actccgcgaa ggcctgttcc cattttgccg
     1201 agagcgccac cagcggcagg tatccgcccg ggctggtgtg ctgggcacaa agctgacgcg
     1261 acaggcgcgc gcgcacatgt tcggcgagca tcaccgggtt gcgcgagcct gcgatgccgt
     1321 cggcaatccc ctccaggatc gtggcgagat cgcggacgga gacgcgttcg gccaggagaa
     1381 tctgaagcac acgttgaatg ccggaaaccg tgatcaggtt gggaaccaga tccttgacca
     1441 ggtcggcttg ctccttcggc agttccttga gcagtttttg cacctcgcca taggacagca
     1501 actccgccat gttgctcttg agcaactctg taagatgcgt ggatagcacg gtggcggcgt
     1561 cgaccaccgt gtagcccttc atcgacgcat cctccttgtg cgaggcatcg atccatgtcg
     1621 ccggcaagcc gaaggtcggt tcggtcgtgt gggttccggg cagtttcacc tgcccgccgg
     1681 ccgggtccat gaccatgtac tggcccgccc agaccttgcc gctgcctgcc tcgacctcct
     1741 tgatcttgat gacataggtg ttcgcatcga gttggacgtt gtcgagcagc cgcaccgccg
     1801 gcatgacaaa gcccatctcg acggcgagag agcggcgcaa agccttgatc tgctcggtga
     1861 ggcggtcgga cccgtcgggc gcattgacca ggggcagcag tgcgtagccg agttctacct
     1921 tcaggtcgtc gatcttgagc gccgcggcaa tcggttcgtc cgcggcttca accttggcgg
     1981 cggcttcggt cttctgcgct tcggtggcga gagtaagctt catgcgcctg tcgacaatga
     2041 aggcgagggc ggctgctccg ctgccaagcg ccatgaacgg aatgagcggg ataccgggca
     2101 aaagcgacat caccagcatc accgcgcccg acatgccgag cgcttttggg tagccggaga
     2161 gctggctgag gagcgccttg tcggcggcgc cggcgacgct tgccttggag acaaggaggc
     2221 cggcagcggt cgaaacgatg agcgccggca cctgcgttac cagaccgtca ccgacggtga
     2281 ggattgtgta ggagtgcgcc gcgtcggaga agctgagccc ctgttgggcg ataccgatga
     2341 tcatgccgcc gatgacattg atgaacacca ccagcagacc ggcaatggcg tcgccgcgca
     2401 caaatttgga agcgccgtcc atcgcgccga agaaaccgcc ttcatcttcc agtttctgcc
     2461 ggcggcggcg cgcctctttc tcgtcgatga gaccggccga aaggtcggcg tcgatcgcca
     2521 tctgcttgcc gggcatggcg tccaaatgga aacgcgcggc gacttcggcg atgcggccgg
     2581 agcccttggt gatgacgacg aagttcacga tgatgaggat cgtgaagacg atgataccga
     2641 tcacgaaat
//
LOCUS       DASSRL010000146         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_738995, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000146 DASSRL010000000
VERSION     DASSRL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738995"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..170
                     /locus_tag="VFL49_03075"
     CDS             6..170
                     /locus_tag="VFL49_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase, NapE protein"
                     /protein_id="HET7910987.1"
                     /translation="MTGDRPQKRRMEIYAFLIITAVLMPALAVATVGTWGLTVWVYQA
                     IAGPPGPPSK"
     gene            179..502
                     /locus_tag="VFL49_03080"
     CDS             179..502
                     /locus_tag="VFL49_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HET7910988.1"
                     /translation="MGESRKSESFNRRKFINGHWMARAGEKSGDVEIASVLVQARPER
                     LDAAARAIEALPGTQIYSRDARGKLVVVIEAADTGSIGATLNAISSLPHVLTASLVFQ
                     GSDAG"
     gene            528..3032
                     /gene="napA"
                     /locus_tag="VFL49_03085"
     CDS             528..3032
                     /gene="napA"
                     /locus_tag="VFL49_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HET7910989.1"
                     /translation="MKKKRLDRREFLKLEAAAMAAAAGGLPAPLAAQNLVTDRSFTEL
                     KWNKAPCRFCGTGCGVMVATKDGRVVATHGDTQAEVNRGLNCVKGYFLSKIMYGHDRL
                     THPLLRKKNGKYDKNGDFAQVSWDEAFDVMAQQFKNALAKGGPTTVGMFGSGQWTIWE
                     GYAANKLFKAGFRSNNIDPNARHCMASAVMGFMRTFGMDEPMGCYDDFEAADAFVLWG
                     SNMAEMHPILWSRVTDRRLSAPHVRVAVLSTFEHRSFELADLEIVFTPQTDLAILNAI
                     ANYIITTNRVNKDFVNKHTVFKRGQADIGYGLRPDNPLEKKAAGRSKPGEATDITFED
                     YAKFVSEYTLEKASSMSGVPVTRLRELAELYADPKRKVVSFWTMGFNQHTRGVWCNNL
                     VYNIHLLTGKISEPGNSPFSLTGQPSACGTAREVGTFSHRLPADMLVANKAHRDVAER
                     IWKLPEGTIVPQPGYHAVLQNRMLRDGKLNAYWVQVNNNLQAAANFEEEGYPGYRNPD
                     NFIVVSDAYPTVTAQAADLVLPTAMWVEKEGAYGNAERRTHFWHQLVSAPGEARSDLW
                     QLMEFSKRFKIEEVWPADLIAKKPEYKDKTLFDVLFKNGEVDKYPTSEIATGFENHES
                     KAFGFYVQKGLFEEYAAFGRGHGHDLAPFDRYHEVRGLRWPVVDGKETRWRYREGLDP
                     YVKPGEGVRFYGFPDGKARIFALPYEPPAESPDTTYPYWLVTGRVLEHWHSGSMTRRV
                     PELYRAFPDAVCFMHPDDAAKLNMRRGDEVKVESRRGLIRTRLETRGRNKMPRGVVFV
                     PWFDESQLINKVTLDATDPISLQTDFKKCAVRITRV"
     gene            3044..3511
                     /locus_tag="VFL49_03090"
     CDS             3044..3511
                     /locus_tag="VFL49_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HET7910990.1"
                     /translation="MGHNLRLFAAATLVAAFSTVVMAQTFSTLRGATPLNQEGPPPVM
                     TPEQNSSIREPRNYPEQPPVIPHSTEGYEISANANKCLSCHARSRIRESQAPMISITH
                     FADRDGQFLASISPRRYFCTQCHVPQHNVRAPIENDFVDIDTLLSRGKPGAPR"
     gene            3508..4140
                     /locus_tag="VFL49_03095"
     CDS             3508..4140
                     /locus_tag="VFL49_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HET7910991.1"
                     /translation="MTTTEAPEPSFWRRALDFAFKIWDVLRKPSSVFSLGFLVLAGFV
                     AGIIFWGGFNTALELTNTETFCVSCHEMRTNVFEELKTTVHYSNRSGVRAHCPDCHVP
                     HDWTDKIARKMQASKEVWGKLFGTINTRDKFLDHRLELAMHEWARMKANDSLECRNCH
                     SAQSMDITRQAPRAIEAHQRFLFTGEKTCIDCHKGVAHHLPDMRNVPGWQ"
     gene            complement(4160..>4568)
                     /locus_tag="VFL49_03100"
     CDS             complement(4160..>4568)
                     /locus_tag="VFL49_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET7910992.1"
                     /translation="VSAVNITLHALVMTVVVRVAQKASERHTSRRPTHVTKVMIPTVL
                     VLMITHSLEVIVWAFAYLLVGAAPEDTDWVYFAFVNYTTLGYGDVVPVARWQLLGPLT
                     AMNGVLLFGWSTAVIFEVLRRALAQGHNVPTRR"
BASE COUNT          974 a         1417 c         1342 g          835 t
ORIGIN      
        1 aaggtttgac cggcgacagg ccgcaaaagc ggcgtatgga aatttatgcc ttcctcatca
       61 ttaccgctgt cctgatgccg gctcttgccg ttgcaaccgt cgggacgtgg ggtctcaccg
      121 tctgggtcta ccaggcgatc gctggtccgc ccggtccgcc gtcgaagtga gccatacaat
      181 gggcgagtct cggaaatcgg aaagcttcaa ccgtcgcaag tttatcaacg gccactggat
      241 ggcccgcgcg ggtgaaaaat caggtgacgt tgaaattgcc agcgtcctcg ttcaggcgcg
      301 gcctgaacgg cttgatgcgg ccgcgcgcgc aatcgaggcc ctgcccggca cgcaaatcta
      361 cagccgcgat gcgagaggaa agctcgtcgt ggtgatcgag gccgccgata ccggcagtat
      421 cggcgccacg ctcaatgcca tttcgtcact accgcatgtg ctcactgcct cgctcgtctt
      481 tcagggcagc gacgcaggct gaacaagatg ccaatgggag gccggcgatg aaaaagaaac
      541 gactggaccg ccgcgaattc ctgaaactgg aagcggctgc gatggccgcc gccgccggtg
      601 gccttcctgc tccgcttgca gcgcaaaacc ttgtcactga tcgctccttc acggaattga
      661 agtggaacaa ggcgccctgc cgtttctgcg gtaccggctg cggcgtcatg gtcgcaacga
      721 aagacggtcg cgtcgtcgca acgcatggcg acacccaggc cgaagtcaac cggggtctca
      781 attgcgtcaa aggctatttt ctctcgaaaa tcatgtacgg ccacgaccgg ctgacgcatc
      841 cgctcctgcg caagaagaac ggcaaatacg acaagaacgg cgacttcgcc caggtgtcct
      901 gggacgaagc cttcgacgtg atggcgcagc aattcaagaa tgcgctcgcc aaaggtggcc
      961 cgacgacggt cggaatgttc ggatcgggtc aatggacaat ctgggaaggt tacgcggcca
     1021 acaagctgtt caaggccgga tttcggtcca acaacatcga tccaaatgcg cgtcactgca
     1081 tggcgtctgc cgtcatgggc ttcatgcgca ccttcggcat ggacgagccg atgggatgct
     1141 acgatgactt cgaggccgcc gacgccttcg tgctgtgggg ctcgaacatg gcagagatgc
     1201 atccgattct ctggagccgg gtcaccgacc gccgtctcag cgcgccgcac gtccgcgtcg
     1261 cggtgctatc gacgttcgag catcgctcgt tcgagctcgc cgatctcgag atcgttttta
     1321 cgccgcagac cgatctcgcg attctgaacg cgattgcgaa ttacatcatc acgaccaacc
     1381 gggtgaacaa ggatttcgtc aacaagcaca ccgtgttcaa gcgcggccag gcggatatcg
     1441 gctacggttt gcggccggac aatccgctgg agaaaaaggc ggccgggcgc tccaagcccg
     1501 gcgaagcaac cgacatcacg ttcgaggatt acgcaaagtt cgtgtcggaa tacacgctgg
     1561 agaaggcgtc cagcatgtcg ggcgtgccgg tcacccgcct tcgcgagctg gcagagctgt
     1621 acgccgatcc gaaaaggaag gttgtgagct tctggacgat gggcttcaat cagcacacgc
     1681 gcggcgtctg gtgcaacaac ctcgtctaca acattcacct gctgacggga aaaatctcgg
     1741 aaccgggcaa cagccccttc tcgctcaccg gtcaaccgtc ggcatgcggc acggcacgcg
     1801 aggtcggcac attctcgcac cgcttgcctg ccgacatgct ggtggcgaac aaggcccacc
     1861 gcgacgtcgc ggagcggatc tggaaattgc ccgaaggcac gatcgtgccg cagccgggct
     1921 accacgcggt gttgcagaac cgtatgctgc gcgacggcaa gctcaatgcc tattgggtgc
     1981 aggtcaacaa caacctccag gccgccgcca acttcgagga agagggatat cccggctacc
     2041 gcaacccgga caatttcatc gtcgtctccg acgcctatcc gaccgtcacc gcgcaggcag
     2101 cggacctggt cctgccaacc gccatgtggg tggaaaagga aggcgcctat ggcaacgccg
     2161 aacgccgcac gcacttctgg caccagctgg tgagtgcgcc gggcgaggcg cgctccgatc
     2221 tctggcagct tatggaattc tccaagcgct tcaaaattga ggaagtctgg ccggccgatc
     2281 tgatcgccaa aaaaccggaa tacaaggaca agacgctatt cgacgttctg ttcaagaacg
     2341 gcgaggtcga caaatatccg acgtccgaaa ttgccaccgg cttcgaaaac catgagtcga
     2401 aagcgttcgg cttctatgtc cagaaaggac ttttcgagga atatgccgcc ttcggccgcg
     2461 gccacgggca cgacctcgca cccttcgacc gctatcacga ggtgcgtggg ctgcgctggc
     2521 ccgtcgtcga cggcaaggaa acgcggtggc gttaccgcga aggccttgat ccttacgtga
     2581 agcccggcga gggagttaga ttttacggtt ttcccgacgg caaagcgcgc attttcgcgc
     2641 ttccctatga gccgccggcg gagtcgcctg acaccaccta cccctattgg ctggtcactg
     2701 gccgcgtact cgagcactgg cactcgggtt cgatgacgcg ccgtgtgcct gagctctacc
     2761 gcgcgttccc ggacgccgtt tgcttcatgc accccgacga tgcggcgaag ctcaatatgc
     2821 gccgcggcga cgaggtgaag gtggagtccc gccgaggcct catccgcacc cggctggaga
     2881 cgcgtggacg caacaagatg ccgcggggag tcgtcttcgt gccgtggttc gacgagagcc
     2941 agctcatcaa caaggtgacg cttgacgcga ccgatcctat ctcgctgcag actgacttca
     3001 aaaaatgcgc ggtgcgcatc acgcgcgtgt agggagcgtc accatggggc acaatctccg
     3061 actgttcgcc gcggcaacgc tcgtggcggc tttttccacc gtggtgatgg cacagacctt
     3121 ctcgacgcta cgcggcgcga ctccgctcaa tcaggaagga ccgccgccgg tgatgacccc
     3181 cgagcagaac tcctcgattc gcgaaccaag aaactatccc gaacagccac cggttattcc
     3241 gcattcgacc gaaggctatg agatcagcgc caatgcaaac aaatgcctct cctgccacgc
     3301 acgaagccgc atacgcgagt cgcaggcgcc gatgatcagc attacgcatt tcgcagaccg
     3361 cgatgggcaa ttcctggctt cgatctcgcc gcgccggtac ttctgcacgc agtgtcacgt
     3421 tccccagcac aatgtgcgag ccccgattga aaatgacttc gtcgatatcg acaccctctt
     3481 aagtcgcggc aagcccgggg cgccgcgatg acgacgaccg aggcgccgga gccctcgttc
     3541 tggaggcgcg cgctcgattt tgccttcaaa atctgggatg tgctgcgtaa gccgagctcg
     3601 gtattcagcc tgggctttct tgtgctggcc ggctttgtcg ccggaatcat tttctggggc
     3661 ggcttcaata cggcccttga gctgaccaac accgagactt tctgtgtctc atgtcacgag
     3721 atgcggacga acgtgttcga ggaactcaaa acgacggtgc attactccaa ccggtccggt
     3781 gtgcgcgcgc actgccccga ttgccacgtg cctcacgact ggacggacaa gatcgcccgc
     3841 aaaatgcagg catcaaagga ggtctggggc aagcttttcg gcaccatcaa tacgcgcgac
     3901 aaattcctcg atcatcgcct cgaacttgcg atgcatgaat gggcacggat gaaggcaaac
     3961 gattccctgg aatgccgcaa ctgccacagc gcgcaatcga tggatatcac gcgtcaggcg
     4021 ccgcgcgcga tagaggcgca tcagcgcttt ctgtttaccg gcgaaaagac ttgcatcgac
     4081 tgtcacaaag gcgtcgctca ccatctgccc gacatgcgca acgtcccagg ctggcaatag
     4141 ccggcatcgc gagagcaatc tagcgacgtg tcggaacgtt atgtccttgc gcgagcgctc
     4201 tgcgcagaac ctcgaagatt accgcggtcg accaaccgaa cagcaggacg ccgttcatcg
     4261 ccgtgagcgg gccaagcagt tgccagcgcg caaccggcac gacgtccccg tacccgagcg
     4321 ttgtgtagtt cacgaaggcg aaatacaccc agtccgtatc ctctggtgcg gcaccaacca
     4381 gaagataggc aaaagcccag acgatgactt caagcgaatg cgtgatcatg agaacgagca
     4441 cggtcgggat catgaccttt gtcacatgcg ttgggcgacg ggacgtatgc cgctcgctgg
     4501 ctttttgcgc gacccgcacc acaacagtca tcaccaaggc atgcagggtg atgttcaccg
     4561 cactgacc
//
LOCUS       DASSRL010000147         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_581361, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000147 DASSRL010000000
VERSION     DASSRL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581361"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..>2504
                     /locus_tag="VFL49_03105"
     CDS             311..>2504
                     /locus_tag="VFL49_03105"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910993.1"
                     /translation="MSAQQALTLPSPAASNTVVINARCSLRIEADQRVVVIAGLPVHH
                     YRAEDAVAEAYAMVFLAESGFAQQTELARAFARSVRSVRRYQRRYAQGGMAALGREEG
                     WRRGRRRISGKRLRGIETLKSQGISNRVIAHRLGVSEKAIRKLIGASKPAADAQLAFA
                     ELTKRPATPVPFATSTGDDVDRIAPAVDERTVERDPIAAPGDEGDPVPMSLDRDASDR
                     SLDRQLAYLGLLDDAAPLFRDGSSVPAVGVLLALPCLVESGLLRISRKLYGEIGPAFY
                     GLRTTLLTLLLMALLRIKRPEHLKERDPAAFGRLLGLDRAPEVKTLRRRLTRLAAHHR
                     AEQLGAELARLRVDQRGRLMGFLYVDGHVRAYHGRRAIASRAYVARRHLAMPASTDYW
                     INDRSGDPLFVITGEVNAALTRALPRLLREVRDVVGERRVTIVFDRGGWSPKLFDTII
                     KEGFDLLTYRAGRCRRIHERRFVRRRAKLDRCWVDYLLHDQPVRFLKGKLRLRQVTCL
                     CDNGHQTQVITSRWDLRDIEVAYRMFDRWRQENFFKYMREEFLLDALVDYQIEPEDPT
                     RTIPNPERRTLDKEIQVARADLARLEREYGAAAATNAEQRRPTMRGFKIAHGKLGKQL
                     RIARSRVAQLCAQRQDLPTRVGVRDLNERTMVRLATERKHLTDIIKMIAYQAESDLLA
                     LLRPHYARAEQEGRTLLHELFATAGDIRIGESELHITLAPLSSPHRTHA"
BASE COUNT          455 a          787 c          794 g          468 t
ORIGIN      
        1 ccctacaagt ctacgacaaa acagcttcct agggcggatt agcggcgcga agcgacgcgt
       61 aatccgccaa ccggcgggcg aacacggcca tggcggatta cgcatcgccg caaagcgcgg
      121 cgatgctaat ctaatttcag acctcctgac atagccggtt tccacgactg aaatcactgg
      181 ctttttcgcg tccgaaggtg cggaccaata catgtccggt ttttctggac tgcaagacgc
      241 aatcggttga agtcgcacaa gttttgcggc ggctcttgcg gacccaaact tgtccgcttt
      301 agccaggcgc atgagcgccc aacaagcttt gacgctaccc tcaccggcgg cgtcgaacac
      361 cgtggtgatc aatgcgcgct gcagcttgcg aattgaggcg gatcagcgcg tcgtcgtgat
      421 cgccggactt cccgtgcatc attatcgcgc cgaggatgcg gtcgccgagg catatgcgat
      481 ggtgttcttg gcggagtcgg gctttgcgca gcaaacggag ctggcgcggg cgtttgcccg
      541 atcggtacgc agcgtgcggc ggtaccaacg tcgctatgcg cagggcggca tggcggcgct
      601 cgggcgcgag gaaggttggc gccgcggtcg gcggcgtatc tccggcaagc gtctgcgcgg
      661 catcgagaca ctgaagagtc aggggatcag caaccgggtg atcgcgcacc ggttgggcgt
      721 cagtgagaag gcaatccgca agctgattgg agcctcgaag cccgccgcgg atgcgcagct
      781 tgcgttcgca gagctgacga agcggccggc gacgcctgta ccgtttgcaa cttcgaccgg
      841 cgatgatgtg gatcgcatcg caccagcggt ggacgagcgc accgtcgagc gcgatccgat
      901 cgccgcgcct ggcgacgagg gtgacccggt accgatgagt cttgaccgcg atgcgagcga
      961 tcggagcttg gatcggcagc tcgcctatct ggggctgctc gatgatgccg caccgctgtt
     1021 tcgcgacggg tcttcggttc ccgcggtcgg tgtcctgctc gcgcttccgt gcttggtcga
     1081 gagcgggctg cttcggatca gccgcaagct ctacggcgag atcggtccag ccttctatgg
     1141 cctgcgcacg acgttgctga cgctcctgct catggcgctg ctgcgcatca agcggcccga
     1201 gcatctcaag gaacgcgatc cggctgcttt cggcagactg cttgggctcg accgcgcgcc
     1261 ggaagtgaag acgctgcgcc ggcgtctgac ccgactcgcg gcgcaccatc gcgccgagca
     1321 gctgggcgcc gagcttgctc gtttgcgtgt cgatcagcgc ggacgtctga tgggattttt
     1381 atacgttgat ggccatgtgc gcgcctatca cggccggcgc gccatcgctt caagggccta
     1441 cgtggcgcgc cgtcatttgg ccatgccagc cagcaccgac tactggatca acgatcgttc
     1501 cggcgatccg ttgttcgtga ttaccgggga ggtcaacgcg gcgttgacga gggcgctgcc
     1561 ccggctgctt cgcgaagtgc gcgatgtcgt gggcgagcga cgggtcacca ttgtcttcga
     1621 ccgcggcggc tggagcccga agctgttcga cacaataatc aaggaaggct tcgacttact
     1681 gacctatcgc gccggccgct gtcgccgcat ccatgagcgg cggttcgttc ggcgtcgtgc
     1741 caaactcgac aggtgttggg tggattatct tctccacgac cagcccgtgc gcttcctcaa
     1801 aggcaagctg cgcttacgtc aagtcacctg tttatgcgac aacggtcatc agacccaggt
     1861 gattaccagt cgatgggacc tgcgcgatat cgaggtcgcc taccgcatgt tcgatcgctg
     1921 gcggcaggag aacttcttca agtacatgcg agaagagttc ctgctcgacg cactggtcga
     1981 ttaccagatc gagccggagg atccgacccg cactattcct aatcccgagc gccgcacttt
     2041 ggacaaggag atccaggtcg cgcgtgccga ccttgccagg ctcgaacgcg aatacggtgc
     2101 tgccgccgcg accaatgccg agcagcgccg cccgaccatg cgcggcttca agatcgccca
     2161 tggcaagctc ggcaagcagc tgcgcatcgc ccgcagtcgc gtggcgcagc tatgcgcaca
     2221 acgccaagac cttcccaccc gcgtcggagt ccgcgacctc aacgagcgga ccatggtcag
     2281 gctcgccacc gaacgtaagc acctgaccga catcatcaag atgattgcct atcaggctga
     2341 gagcgatctg cttgcattgc tgcggcctca ctacgcccgt gccgagcaag agggacgaac
     2401 tctgctgcat gagctgttcg ccaccgcagg agacatccgc attggcgaga gcgagctgca
     2461 tatcaccttg gcgccgctca gctcgccgca tcgaacgcac gccg
//
LOCUS       DASSRL010000148         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_389068, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000148 DASSRL010000000
VERSION     DASSRL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389068"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /gene="atpA"
                     /locus_tag="VFL49_03110"
     CDS             complement(<1..1248)
                     /gene="atpA"
                     /locus_tag="VFL49_03110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7910994.1"
                     /translation="MQIKAEEISSIIKEKIKGFDKQVDVKETGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALNLDEDNVGAVLMGDDVGIKEGDPVKRTGRIAEIPVGEA
                     LVGRVVNAIGQPIDGKGPIKSQLSSRIEVVAPGVITRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLGVFCIYVAVGQKRSTVARVVKTLEEHHAM
                     DYSMVVSATASDAAPMQFLAPFAGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFAQFGSELDKATQRQLARGVRM"
     gene            complement(1339..1878)
                     /gene="atpH"
                     /locus_tag="VFL49_03115"
     CDS             complement(1339..1878)
                     /gene="atpH"
                     /locus_tag="VFL49_03115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET7910995.1"
                     /translation="MIKTAVARRYAQALFELLDRSNIEATRGSLNGLAQALKDSSSLR
                     HVVASPAFGAEDKVAVLSELASRLGGAAAVKPFLGQLVKKNRVGFLPDIADAFAKLVD
                     RSKGTQPVTVFSAAALPAAEQDRIKTRLRETLKREVDVTFQTDAAYLAGLQIHIGSMV
                     VDSTVRGRLDAMEALLTRD"
BASE COUNT          404 a          637 c          630 g          428 t
ORIGIN      
        1 catgcgcacg ccgcgcgcga gctgccgttg cgtcgccttg tccaattcac tcccgaactg
       61 cgcgaaggcc gccatttccc gatactgggc aagatccagg cgcagcgtgc ccgcgacctg
      121 cttcatcgtc ttgatctggg cggatccacc gacgcgcgac accgacaaac caacgttgat
      181 cgcgggacgg atgcctgaat agaagagatc gctgccgaga tagatctgac cgtcggtaat
      241 cgagatgacg ttcgtcggaa tgtaggccga cacatcgccc gcctgcgttt caatgatcgg
      301 aagcgccgtc aagcttccgc cgcccagctt gtcgctgagt tttgcagcgc gctcgagcag
      361 gcgtgagtgc aggtagaaca catcgccggg atacgcttcg cggccggggg gacgccggag
      421 gagcagcgag agttgccggt aggcgaccgc gtgctttgaa agatcgtcat agacgatcaa
      481 ggcatgcttg ccgttgtctc gaaagtactc gccgatggcc gcgccggcga acggagccaa
      541 gaactgcatc ggagcggcat cgctggccgt cgccgatacg accatgctgt agtccatggc
      601 atggtgctct tcgagagttt taaccacccg ggcgaccgtc gaccgctttt gaccgaccgc
      661 gacgtagatg cagaagacgc cgaggccttt ttgattgatg atcgtatcca ccgcgatggc
      721 cgtcttgccc gtttgccggt cgccgatgat caattcccgt tggccgcggc cgatgggaat
      781 catcgcgtcg atcgccttga tgccggtttg caggggttcc cggaccgatt gccgggtgat
      841 gacgccggga gccacgacct cgatgcgcga agagagttgc gatttgatcg ggccctttcc
      901 gtcgatcggc tgtccgatcg cattgaccac ccggcccacg agggcttcgc cgacgggaat
      961 ttcggcaatg cggcccgtgc gcttgacagg atcgccttcc ttgatgccga catcgtcacc
     1021 catcaagacg gcgcccacgt tgtcctcatc gaggttcagc gcgatgccgt agaggccgcc
     1081 gggaaattcg agcatttcgc cggccatggc tccatcgagt ccgtagacct tggcaatgcc
     1141 gtctccgacc tggatcaccg agccggtttc cttcacgtcg acctgcttgt cgaagccctt
     1201 gattttttct ttgatgatcg agctgatttc ttcagcctta atctgcatgg cgactcctta
     1261 gcttcttcag tatcgtgaga gcacgcgccc ggtgcggttt gcgtgccgga ccttcactcg
     1321 ttgcgcgtgg tgatcgacct agtcccgcgt gagcagcgcc tccatggcgt ccaaacgtcc
     1381 gcggacggtg ctgtccacga ccatactgcc gatgtggatc tgcaagccgg cgaggtaggc
     1441 ggcatcggtc tgaaaggtca catcgacttc tcgcttcaac gtctcgcgca ggcgcgtttt
     1501 gatacggtct tgttcggcgg ccggcaaggc agcggccgaa aagacggtca ccggttgggt
     1561 acccttggac cggtctacca gtttggcaaa tgcgtcggca atatcaggca aaaatccaac
     1621 acggtttttc ttgaccagct gtccgagaaa gggcttcacg gccgcggccc cccccaagcg
     1681 gctggcaagc tccgacagta cagcgacctt gtcttcggcc ccaaaggccg gcgaagcgac
     1741 gacatgacga agggacgacg agtccttaag cgcctgagcc aagccgttga ggctcccccg
     1801 cgtcgcttcg atgttagatc gatcgagaag ctcgaagagc gcctgagcgt aacgtcgcgc
     1861 aactgccgtc tttatcactg tcctaatcca ctcattggaa atttagggca aacgggatgc
     1921 ttccgtgcgg gaggcacgaa aatcggcgcg caaacgtagc attcgctgac gtgaagtgtc
     1981 aatacagtcg ggcaacagcg acggctctag caggattgga taatgccgcc cccgatcacc
     2041 tgatcgccaa aatagaatac cgccgattgc ccgggactga gtgcgcgttg agccgtttg
//
LOCUS       DASSRL010000149         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_616435, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000149 DASSRL010000000
VERSION     DASSRL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616435"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2168
                     /gene="rbbA"
                     /locus_tag="VFL49_03120"
     CDS             <1..2168
                     /gene="rbbA"
                     /locus_tag="VFL49_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HET7910996.1"
                     /translation="RVRARRTGMSVIVATAYMEEAERFDWLAAMSEGRVISTGTAGAL
                     KERTGADNLDAAFIQLLPEEQRARHETVVLPPRTGTGGAPVIEATNLRCRFGDFTAVD
                     RVSFQIEKGEIFGFLGSNGCGKTTTMKMLTGLLPASAGEATILGRPVDATDLNTRRHV
                     GYMSQGFSLYSELTVRQNLKLHAELFHLPAEKVGPRIAEMLHRFGLSDVADELPPSLP
                     LGLRQRLSLAVALIHKPDLLILDEPTSGVDPVARDEFWRSLIELSRKDGVTIFISTHF
                     MNEAERCDRVSLMHAGRVLAVGSPADLAHSKETETLEDAFITYLKEVDAAIGVPAPPV
                     EAVRHSGEALRVSRFSMTRLRAFASREMTEVLRDPIRLAFALLGPLVLLMTFGFGITF
                     DVERLPFAVLDNDRSPESRELVETLGGSRYFVKQPAVTDLADLERRLKGGRLSVALEI
                     PAGFGRDFWRGADPEVAVWLDGATPFRAETARGYLEGLVLQYAEDLAARRGIDSDVLP
                     VSFEIRLPFNQRFESVYAIAPGIIMMLLAIIPSMMTALGVVREKEIGSIVNFHATPVS
                     PFEFLVGKQLPYVAIALGNFLSLWLLAALVFRIDLTGSVSALVIGAICYVAATTAFGL
                     LVSTFVRTQIAAVFATSIVIMIPTVNFSGFMTPISSLDEAGRLFGLAFPGAYFQQISI
                     GTFTKGLRWDDLWHNHLVLAGFAIGYVLISILLLRKQEA"
     gene            2170..>3234
                     /locus_tag="VFL49_03125"
     CDS             2170..>3234
                     /locus_tag="VFL49_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7910997.1"
                     /translation="MEQGIANVFRLGVKELRSLYADPVLLLLMLYTFTFAVYQIAQNV
                     HMEVTDAAVAIVDEDHSRLSHRIADAILPPQFKPPPELNSDELDPGLERGEFIFALTI
                     PHGFERDLMDGRKPALQLDVDATAMSMAGNGAVYLESIVLRELAAAQNTDLNISSLPF
                     QVVTRAAFNPNLSSRSYMAIMQLTENITIMAIILTGAAFIREREHGTIEHLLVMPVTP
                     FEIMCAKIWANGAAVLTATALSLYAVVRGLLAIDLPGSAPLFLAGTGLYLFSATALGI
                     FLATLARSMPQFGLLSIPVFTVMNLLSGAATPLESMPEWLQTTMQISPSTHFVKFAQS
                     VLFRDAGLDLVWRHLLPIAGL"
BASE COUNT          548 a         1009 c          983 g          694 t
ORIGIN      
        1 accgcgtgcg ggcgcgccgc acgggcatga gtgtaatcgt tgccaccgcc tacatggagg
       61 aagcggaacg gttcgattgg ctggcagcca tgtccgaggg ccgggtcatc tcgaccggca
      121 cggccggcgc gctgaaggag cgcaccggcg cggacaacct ggacgcggca tttatccagc
      181 ttctgccgga ggaacagcgc gcgaggcacg aaacggtggt tctgccgccg cggaccggca
      241 ccggtggtgc gccggtgatc gaagccacga atctcaggtg ccggttcggc gacttcaccg
      301 ctgtggaccg ggttagcttc caaatcgaaa agggcgagat cttcggtttc ctcgggtcca
      361 acggctgcgg caagacaact acgatgaaga tgctgaccgg ccttctgccg gccagtgcgg
      421 gagaggcgac tattctcggt cggccggttg atgccaccga cctcaatacc cgtcggcacg
      481 tcggctacat gtctcaaggt ttctcgctct attccgaatt gaccgtgcgg cagaacctga
      541 agcttcatgc cgagctgttc catctaccgg ccgagaaagt cggaccgcga atcgccgaga
      601 tgctgcatcg gttcggcttg agcgacgtcg cggatgaact gccgccgtcg cttccgctcg
      661 ggctgcggca acgcctgtcc ctggcggtgg cgctgatcca caagccagac ctgctcatcc
      721 tcgatgagcc gacctccggg gtcgaccccg ttgcgcgcga cgagttctgg cgatcgctga
      781 tcgagctgtc gcgcaaggat ggtgtcacga tcttcatctc cacccatttc atgaacgagg
      841 ccgagcgctg cgatcgcgtg tcgctgatgc atgccggccg cgtcctcgcg gtcggcagtc
      901 cggcagacct ggcgcacagc aaggaaaccg agaccctgga ggatgctttc atcacctatc
      961 tcaaggaggt cgatgccgcc ataggcgtgc cggcaccgcc agtagaagcg gtgcgccatt
     1021 ccggagaggc actgcgagtc tcgcgcttca gcatgacccg tctgcgggcg tttgccagtc
     1081 gtgagatgac ggaggttttg cgcgatccga tccggttggc cttcgcactc ctcggaccgc
     1141 tggttctgct aatgaccttt ggcttcggaa tcacgttcga cgtcgagcgc ctgccgttcg
     1201 cagtgctgga caatgatcgc tcaccggaaa gccgggaatt ggttgagacg cttggcggtt
     1261 cgcgctattt cgtaaagcaa ccggccgtca cggacttggc tgatctggaa cgtcgcctga
     1321 aaggcggtcg gctgagcgtc gcgctcgaga tcccggcggg cttcggccgc gacttctggc
     1381 gcggagccga ccccgaagta gcggtgtggc ttgatggggc aacgccgttc cgcgccgaaa
     1441 cagcgcgcgg ttacctggaa ggcctggtcc tgcagtatgc ggaggatctt gccgcgcgcc
     1501 gcgggatcga cagcgacgtg ctgccggttt ccttcgagat cagactccct ttcaaccagc
     1561 gattcgagag tgtctatgcc atcgcgcctg gcatcatcat gatgctgctg gccatcatac
     1621 ccagcatgat gacagcgctt ggcgtcgtgc gcgagaagga gatcggctca atcgtcaact
     1681 tccatgcgac accggtcagc ccgttcgagt tcctggtggg caagcaactg ccctatgtcg
     1741 ccatcgctct cggcaatttc ctttcgctct ggctcctggc ggcgctggtg ttccggatcg
     1801 atctgactgg ctcggtttca gcgctggtca tcggcgctat ctgctacgtg gcggcgacca
     1861 ccgcctttgg cttgttggta tcgacctttg tcaggactca gatcgcggcg gtctttgcca
     1921 cctcgatcgt catcatgatc ccgaccgtca acttctcggg cttcatgacg ccgatctcgt
     1981 cattggatga ggcaggccgc ctgttcggcc tcgcttttcc gggcgcttac ttccagcaga
     2041 tcagcatagg gactttcacc aagggcctac gttgggatga cctctggcat aaccaccttg
     2101 ttcttgccgg atttgcgatt ggctacgtgc tgatctccat cctcctattg cgcaagcagg
     2161 aggcttgaga tggaacaggg gatcgcgaac gtattccggc taggagtgaa ggaactgcgc
     2221 agcctctacg ctgatcccgt gctgctgttg ctgatgcttt acaccttcac gtttgctgtc
     2281 tatcagatcg cgcagaatgt gcacatggag gtgacggatg ctgctgtggc gatcgtcgat
     2341 gaggatcact cgcggctctc ccatcgcatc gccgatgcga ttcttccgcc gcaattcaag
     2401 ccgccgccgg agctcaactc ggacgagctt gaccccggcc tggaacgtgg cgagttcata
     2461 tttgccctga ccatccctca cggcttcgag cgcgacctca tggatggtcg caagccggcc
     2521 ctccagctgg acgtggacgc aacagccatg tcgatggctg gcaatggcgc cgtctatctg
     2581 gagagcattg tcctgcggga actcgcggcg gcgcaaaata ccgaccttaa catctcgtcc
     2641 ctgccgttcc aagtagtcac gcgcgccgct ttcaatccaa atctcagttc acgctcgtac
     2701 atggccatca tgcagctcac cgagaacatc accatcatgg cgatcatttt gaccggggcc
     2761 gccttcattc gcgagcgcga gcatggcaca atcgagcacc tgctagtcat gccggtgacg
     2821 cctttcgaga tcatgtgcgc taagatctgg gccaacggcg ctgcggtcct gacagctacg
     2881 gcgctgtcgc tgtacgcggt cgttcgcggg ctgctggcga tcgaccttcc gggctcggcc
     2941 ccgctcttcc tggctgggac ggggctttac ctgttctcgg caactgccct cggcatcttc
     3001 ctggcgacgc ttgcgcgctc aatgccgcag tttggccttc tgtcgatccc ggtctttacc
     3061 gtcatgaact tgctttctgg cgccgcgacg ccgctcgaga gcatgcctga gtggctgcag
     3121 acgacgatgc agatctcacc atcgacgcat ttcgtaaagt tcgcgcaatc ggtgctgttc
     3181 cgagatgcgg gcttggatct ggtctggcgc catctcttgc cgattgccgg cctc
//
LOCUS       DASSRL010000150         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_914075, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000150 DASSRL010000000
VERSION     DASSRL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914075"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..441
                     /locus_tag="VFL49_03130"
     CDS             286..441
                     /locus_tag="VFL49_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7910998.1"
                     /translation="MKNDGSGTFGIIVGGVVAAIAIIFILTGGELGGKKTVEGDHDLP
                     PITTTDK"
     gene            complement(448..1923)
                     /gene="gatA"
                     /locus_tag="VFL49_03135"
     CDS             complement(448..1923)
                     /gene="gatA"
                     /locus_tag="VFL49_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7910999.1"
                     /translation="MTELTSLNLAEARDGLKKKSFSACELTAAHIAAIEKARALNAFV
                     LETPERAGEMAKASDNRIASGNAGPLEGIPLAIKDMFCTDGIRSTACSHILDNFVPTY
                     ESTVTSQLWRDGAVLLGKTNCDEFAMGSSNETSHFGPVHSPWRRKGSNTPLTPGGSSG
                     GSAAAVAAKLCLGATGTDTGGSIRQPAAFTGTVGMKPTYGRCSRWGIVAFASSLDQAG
                     PFTRSVRDAAILLRSMAGPDAKDTTCADLAVPDYEAAIGKSVKGLRIGIPKEYRLDGM
                     PAEIEKIWEKGRVWLKAAGAELVEVSLPHTKYALPAYYIVAPAEASSNLARYDGVRYG
                     LRVPGRDIIDMYEKTRSSGFGDEVRRRVMIGTYVLSAGYYDAYYLRAQKVRTLIKKDF
                     EDCFNAGVHAMLTPATPSAAFGLGEKGGTDPVEMYLNDIFTVTVNMAGLPGISVPAGL
                     DSQELPLGLQLIGRPFDEETLFALAAAIERAAGRFEPQPWW"
     gene            complement(2190..2477)
                     /gene="gatC"
                     /locus_tag="VFL49_03140"
     CDS             complement(2190..2477)
                     /gene="gatC"
                     /locus_tag="VFL49_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7911000.1"
                     /translation="MAVDADTVRRIAHLARIAVAEDEIDHLKGELNAMLSFVEQLDEV
                     KVEGVEPMTSVTPMTMKKRADAVTDGGIAEDIVKNAPVSEDNFFLVPKVVE"
     gene            2625..3509
                     /locus_tag="VFL49_03145"
     CDS             2625..3509
                     /locus_tag="VFL49_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7911001.1"
                     /translation="MPIRTHDRYPYSPISARPVYDWPGGKRLAIYIGLNLEWFSFGEG
                     LGAELAPGGPQPDVLNYAWRDYGNRVGVFRLAELFAELKLPVSLLVNAEMYRHAPEAV
                     AAFQDAEIIGHGRTNSERQGTLSEAEERALIAETTEAIEKHCGKRPQGWLGPWISQSP
                     LTPDLLQEFGYRYLLDWCHDDQPVWMKTRRGRILSLPYPQELNDIPQIVARKREGTDF
                     ADMIVDAFEVMQHESKKRPLVMGIALHAYIVGWPHRFKHLARALKHIVDRADRSVWLA
                     TAGNIAAHAEQLPSGTVP"
     gene            3556..>3764
                     /gene="ruvX"
                     /locus_tag="VFL49_03150"
     CDS             3556..>3764
                     /gene="ruvX"
                     /locus_tag="VFL49_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7911002.1"
                     /translation="MSVILPLTEAAAHFPPRGVLLGIDLGTKTIGVAGSDPDRKLATG
                     IETISRKNFSADAQRLLALAAERSAV"
BASE COUNT          729 a         1211 c         1073 g          751 t
ORIGIN      
        1 cgcacccttg accaattttt gcgcattgga cttgacggtc ataataccgc gcgtgcctct
       61 gagctcggaa agcgtgaatc tacatctggt agcggttcat ccgatgcgcg gcaagtttag
      121 gcaagtcgcc cgcaaagaac accgctcgca tgttttggca aagctgaatt gttatccatg
      181 caatccacta cctccaaatg gccaaaattc cgcaacgatt tttgccgatc tcgcgcgcat
      241 cccgccgcag gaaccacgtg cggcgggatg cgttggacgg gtgtcatgaa aaacgacggc
      301 agcggcacat tcggcattat cgtgggcggc gtggtcgccg cgattgcaat catttttatt
      361 ctcaccggcg gagagctcgg cggcaagaaa accgtcgaag gcgatcacga cctgccgccg
      421 attacgacca ccgacaaata ggcggcctta ccaccaaggc tgaggctcaa atcgtcctgc
      481 cgcccgctcg atcgcggctg cgagggcaaa cagtgtttcc tcatcgaatg gacggccgat
      541 caactgcagc cccaacggca gttcctggct gtcgagaccg gcgggaacgg agatgcccgg
      601 cagccccgcc atattcacag tgaccgtgaa tatgtcgttc agatacatct cgaccggatc
      661 ggtgccgccc ttctcgccca gtccaaatgc ggcggagggc gtcgcaggcg taagcattgc
      721 atgcacgccg gcattaaagc agtcctcgaa gtcttttttt atgagcgtgc gtaccttctg
      781 tgcgcgcaag tagtaggcgt cgtagtaccc ggccgagaga acataggtgc cgatcatgac
      841 acgccttcgg acttcgtcgc cgaaaccgct gctccgggtt ttctcgtaca tgtcgatgat
      901 gtcgcggccc ggaacacgca agccatagcg aacgccgtcg tagcgcgcga ggttcgatga
      961 cgcttctgcc ggcgcaacga tatagtaagc cggcagcgca tatttcgtat gcggcaacga
     1021 aacctcgacg agttcggcgc cggccgcttt caaccatacc cgtcccttct cccagatttt
     1081 ttcgatttcc gccggcatcc catcgaggcg atactccttc ggaataccga tgcgcagccc
     1141 tttcaccgat tttccaattg ccgcttcata gtcgggcact gcaagatccg cgcacgtcgt
     1201 gtcttttgcg tccggacccg ccatcgatcg caggaggatc gccgcgtcac ggacgctgcg
     1261 cgtaaacggc ccggcctggt cgagcgacga tgcgaacgca acgatgcccc accgcgagca
     1321 gcggccatag gtcggtttca ttccgaccgt tcccgtgaaa gcggcgggct gacggatcga
     1381 cccgccggta tccgtgccgg ttgcgcccag acacagcttc gcggcgaccg cggcagccga
     1441 gccccccgac gaaccgccgg gcgtcaacgg ggtgttcgag cccttgcgcc gccatggaga
     1501 atgtaccggt ccgaaatgcg aggtctcgtt ggacgagccc atggcgaatt cgtcacagtt
     1561 ggttttgccc aaaagcactg cgccgtcgcg ccagagctgc gacgtcaccg tcgattcgta
     1621 ggtcggcacg aaattgtcga gaatgtgaga acaagcagtc gaacgtatgc cgtcagtgca
     1681 gaacatgtct ttgatggcga gcgggatgcc ctcgagcggc ccggcattgc ccgacgcgat
     1741 gcggttgtcc gatgctttcg ccatctcgcc ggcgcgctcc ggcgtctcca gcacaaacgc
     1801 attaagtgcg cgtgccttct cgatcgccgc aatatgcgca gcggtcaact cacaggcgga
     1861 aaaggatttc ttcttcagcc cgtcccgcgc ttccgccaaa ttcagcgacg tcaattcggt
     1921 cacggtgtcg gctcatccgc cgttcgtcgt gcaggccgct gtagctcggg ctcgggcctt
     1981 tcagtgcctg gctcgcacgc aaaaaccgga tcaccggttg cgctggtctt cttcacggcg
     2041 tcacgttccg cctggagagc gtcaatcatt gcccaatagc cgagctcgca ggcgatacac
     2101 atgctacctc gccatccatg tttctccgcg cgagatcccc gggtctcgcg gcgctcgccc
     2161 cggcatcgtt gaatgatccg cttcgcgtct cattccacca ccttcggtac aagaaagaag
     2221 ttgtcctcgc tcaccggcgc attcttgacg atgtcctcgg cgatgccgcc atcggtcact
     2281 gcgtcagcgc gcttcttcat cgtcatgggc gtgaccgacg tcatcggctc gaccccctcg
     2341 accttcacct catcgagctg ctcgacgaag gagagcatgg cgttcaactc gcccttgagg
     2401 tgatcgattt cgtcctcagc cacagcgatg cgggcaaggt gcgcaatgcg gcgtacggta
     2461 tcggcgtcga cagccattga tgttccggaa atccaacgat ccttcgccag cctatagcag
     2521 agcgtctttc accccgacaa ccggcttggc tggccttcac tctactgcgg cgaatgatgc
     2581 taagtaaggc agagacaaac cgggtttgga cgggctacag acgaatgcca attcgcactc
     2641 acgaccgcta tccctattct cctatttccg cgcggccggt ctacgactgg cccggcggca
     2701 agcgccttgc catctacatc ggccttaacc tcgagtggtt ctcgttcggc gagggactgg
     2761 gcgcagagct cgcaccgggt ggaccgcaac ctgacgtgct caattatgcc tggcgcgact
     2821 atggcaatcg tgtcggcgtc ttccgcctgg ccgagctctt tgcagagctg aaattaccgg
     2881 tctcgcttct ggtcaatgca gaaatgtacc gccatgcgcc agaggcggtc gcggcgttcc
     2941 aggacgccga gatcatcgga cacggccgga cgaattcaga gcgccagggg acgctgagcg
     3001 aagcggagga gcgcgccctg attgccgaaa cgacggaagc gatcgaaaaa cattgcggca
     3061 agcgtccgca aggttggctc ggcccctgga tatcccaatc tccgcttact ccggatcttc
     3121 tgcaggagtt cggctatcgc tacctgctcg actggtgtca tgacgatcaa cccgtctgga
     3181 tgaaaacccg ccggggccgc atcttgtcct tgccctatcc tcaggagctc aacgacatcc
     3241 cgcagatcgt tgcacgcaag cgcgagggaa ctgatttcgc cgacatgatc gtggatgcct
     3301 tcgaggtgat gcagcacgaa agcaaaaagc ggccactggt catgggcatt gcgctgcacg
     3361 cgtacatcgt cggatggccg caccgcttca agcatctggc gcgcgcactc aagcatattg
     3421 tcgaccgtgc cgaccggagc gtctggcttg ccaccgccgg caacattgcc gcgcacgcgg
     3481 agcagcttcc atccggcaca gtaccctgag gtgccgctgt ttggcggcct gaacacctcg
     3541 tgctagcgct accccatgtc ggtcatcctg ccgctcaccg aagccgccgc acattttccg
     3601 ccgcgcggag ttctgctggg catcgacctt ggaacgaaga ccatcggcgt tgccgggagt
     3661 gatcctgatc gcaagcttgc aaccggcatt gaaaccattt cgcgcaagaa tttctccgcc
     3721 gacgcccaac ggctgctcgc gctcgccgcc gagcgctcag cggt
//
LOCUS       DASSRL010000151         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1578772, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000151 DASSRL010000000
VERSION     DASSRL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578772"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFL49_03155"
     CDS             complement(<1..326)
                     /locus_tag="VFL49_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HET7911003.1"
                     /translation="MTVPASLIDRAGLDRAKVRQLVGRGLNGADDGELFLEYRQAEAL
                     TFDNGRLKQATYDTAQGFGLRAVKDEAVGYAHASDLSEAAVARAVEAVRAVKGGHSGT
                     YADAPSR"
     gene            complement(387..935)
                     /locus_tag="VFL49_03160"
     CDS             complement(387..935)
                     /locus_tag="VFL49_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HET7911004.1"
                     /translation="MRFSKHDRHALFGVGWLKAILASLTLIALLALAPADYALAQGAV
                     KSVHKDWQIRCDTPPGAKTEQCALIQSVTAEDRANVGLTVIVLKTADQKSRLMRVVAP
                     LGVLLPSGLGLKIDQADIGRAGFVRCLPNGCIAEVIMDDELIKKLRSGKTATFIIFQT
                     PEEGIGFPMSLAGFGEGYDKLP"
     gene            1353..2192
                     /gene="coxB"
                     /locus_tag="VFL49_03165"
     CDS             1353..2192
                     /gene="coxB"
                     /locus_tag="VFL49_03165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7911005.1"
                     /translation="MLVLKRLIALIVTSVTVIGAGAAVAATGQPQPWQLGLQQGATPV
                     MDDIIWFHDFLLWVISAITLFVLALLVIVMVKFNSRSNPTPSRTTHNTAIEVLWTVVP
                     VVILVTIAIPSFRLLFFQLNVPKADVTVKATGKQWFWSYSYPDSKFEFDSLMVQEKDL
                     KPGQPRLLTVDNEMVVPVNKVVHVLTTGADVIHSFAVPSFGIKIDAIPGRINETWFKA
                     EREGTYHGQCSELCGKDHAFMPITVRVVGEKDYAAWIEQAKKKFANDESRPTAVAAAD
                     GAK"
     gene            2234..>3077
                     /locus_tag="VFL49_03170"
     CDS             2234..>3077
                     /locus_tag="VFL49_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7911006.1"
                     /translation="MASNAAAAHGAHDVHDHHPTGWRRFVYSTNHKDIGTMYLVFALA
                     AGVIGGCLSIAIRMELQNPGIQFFPSSHEFDVFTTAHGLIMIFFMVMPAMIGGFGNWF
                     VPLMIGAPDMAFPRMNNISFWLLPASFALLITSLFVEGEPGSLGSGTGWTLYAPLSTS
                     GHPGPSVDFVILSLHLAGASSILGAINFITTIFNMRAPGMTLHKMPLFVWSILVTVFL
                     LLLALPVLAGAITMLLTDRNFGTTFFANEGGGDPVLFQHLFWFFGHPEVYILILPGFG
                     MISQI"
BASE COUNT          577 a          901 c          965 g          634 t
ORIGIN      
        1 cggctcggcg cgtccgcata agtgccgctg tgaccgccct tgactgcgcg cacagcctcg
       61 acggcacgcg cgacggcggc ttcggaaaga tcggaggcgt gcgcataacc gaccgcttcg
      121 tccttcacgg cgcgcaatcc gaagccttgt gccgtgtcgt aagtagcctg cttgaggcga
      181 ccgttgtcaa aagtgagcgc ttcagcctgg cggtactcga ggaacagttc gccgtcatca
      241 gcgccgttca gtccgcggcc gaccagctgg cgtaccttgg cgcggtccag cccggctcga
      301 tcaataaggg aagcaggaac agtcatggtc ggctccgtca gggcgttcct cttagatagg
      361 gccgcagaca ccagcccgcg agagccttac ggcagcttgt cgtagccttc gccgaatccg
      421 gccaggctca tggggaagcc gatcccttcc tcgggtgtct ggaagatgat gaaagtcgcc
      481 gtcttgcccg aacgcaactt cttgatcagt tcgtcgtcca tgatcacttc ggcaatgcag
      541 ccgttgggga ggcagcgaac gaaaccggcg cgaccgatgt cggcttggtc aatcttgagg
      601 ccgaggcccg acggcagcag aacgccaagg ggcgcgacga cacgcatcag ccggcttttc
      661 tgatcggcag tcttgagcac gatcacggtg aggccgacat tggcgcgatc ctcggcggtg
      721 acgctctgga tcagggcaca ctgctcagtc ttggcgcccg gcggcgtatc gcagcggatc
      781 tgccagtctt tatgcacaga cttcaccgcc ccttgggcaa gcgcgtaatc cgcgggcgcg
      841 agcgccagca gcgcgatcag cgtcagagat gcgagtatgg ccttgagcca gcccacgccg
      901 aaaagcgcat gcctgtcatg cttcgaaaat cgcatcatgg agtccatcgg catccggcta
      961 tcaggtcctt tcgaggtgca gactaggcga atcgtcgcct ggtcggcacg ttatttgttt
     1021 tggttgggtc atgcccggcc ggtcctgtca agcaaggcaa gccgagaggg gtggtgcggc
     1081 ggagatcggc aaagcccgga aatgcgatgc agaaaccggg tttcgggtac gccgtttgtc
     1141 gcagacggga gataatgagg tgcattgcgg cagggcagga attatggttt gagaagtcgg
     1201 gatttttccc cgtcccgccg caccgcccaa gctgccacgc ccgtggcaaa tgccgtgctc
     1261 agcggggtcg cgccgaacat catgccagtg gtgggcggca aaaaaagggg cctgagcagg
     1321 gccgttttct cgtcgaagga gcgcattgga caatgctggt gctcaagcgg ttgatcgcat
     1381 tgatcgtcac gagcgtgacg gtcatcggcg caggtgccgc agtggcggcg acgggtcagc
     1441 cgcagccgtg gcaactcggc ctgcagcagg gtgctacgcc ggtgatggac gatatcatct
     1501 ggttccacga tttccttttg tgggtgattt ccgcgatcac gctgttcgtg ctcgcgctgc
     1561 tggtcatcgt gatggtgaag ttcaactcgc gcagcaatcc gacgccgtcg cgcaccacgc
     1621 acaacacggc gattgaggtc ctgtggaccg ttgttccggt cgttatcctg gtgacgatcg
     1681 ccattccttc gttccgcctg ttgttcttcc agctcaatgt tccgaaggcg gacgtcacgg
     1741 tgaaggccac cggcaagcaa tggttctgga gctacagcta tccggacagc aagttcgagt
     1801 tcgattcgct gatggtgcag gaaaaggatc ttaagccggg ccagccgcgc ctgctcacgg
     1861 tcgacaacga aatggtcgtt ccggtgaaca aggtcgtgca tgtgctgacg acgggcgccg
     1921 acgtgatcca ctcgttcgcg gtgccgtcgt tcggcatcaa gatcgacgcg atccccggcc
     1981 gcatcaacga gacctggttt aaagccgagc gtgaaggcac atatcacggt caatgctcgg
     2041 aactttgcgg caaggaccac gccttcatgc cgatcacggt ccgcgtcgtg ggcgagaagg
     2101 actatgcggc atggatcgag caagcgaaga aaaaattcgc aaatgacgaa agccgtccga
     2161 ccgcggtggc ggcggccgat ggtgcgaagt aacggatacg cgggcgacaa cgacgagtcg
     2221 aaggacaata acgatggcta gcaatgcggc tgctgcacac ggtgcacacg atgtccacga
     2281 tcatcacccg accggatggc gacgtttcgt gtattcgacg aaccacaagg acatcggcac
     2341 gatgtacctc gtgtttgcgc tggctgccgg ggtgatcggc gggtgcttgt cgatcgccat
     2401 ccgcatggaa ttgcagaatc cgggaatcca gtttttccca agctcgcacg agttcgacgt
     2461 gtttacgacc gcacacggcc tgatcatgat ctttttcatg gtgatgccgg cgatgatcgg
     2521 cggtttcggc aactggtttg tgccgctgat gatcggagcg ccggatatgg cgtttccgcg
     2581 catgaacaat atttcgttct ggcttctccc agcctcattt gcactgctca tcacgtcgct
     2641 gttcgtggag ggcgagccag gttcgctcgg ttcgggcacg ggctggactt tatatgcgcc
     2701 gctgtcgacc agcggacatc cgggaccatc ggtcgacttc gtcattttgt cgctgcatct
     2761 ggccggcgcc tcgtcgatcc tcggcgccat caacttcatc acgaccattt tcaacatgcg
     2821 cgcaccgggc atgacgctgc ataagatgcc gctgttcgta tggtcgatcc tggtgacggt
     2881 gttcctgctg ctgctggcgt tgccggtgct cgccggcgcg atcaccatgc tgctcaccga
     2941 ccgcaatttc ggcacgacat tcttcgcgaa cgagggcggc ggcgacccgg tgctgttcca
     3001 gcatctgttc tggttcttcg gccatcccga agtgtacatc ttgatcctgc ccggcttcgg
     3061 catgatcagt cagatcg
//
LOCUS       DASSRL010000152         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_983886, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000152 DASSRL010000000
VERSION     DASSRL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983886"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFL49_03175"
     CDS             <1..607
                     /locus_tag="VFL49_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911007.1"
                     /translation="AIDLARKMPASDTALVATTNVLLARKDVHPAIVDLLAQTTLQAH
                     STPGLFRKVGEFPTETDPEYPMSQGARDFYRNGPSFLNRYLPFWMTNYAQRFLAVLVA
                     VIAIVVPVFNYAPKLFLWLIRDRVRRLYRRLRLVDKALLSPPNSAQIEALNAELENIA
                     RAANIVPMHSSELFFELRAHIDRTRRHLASADAHNGSAKVA"
     gene            608..799
                     /locus_tag="VFL49_03180"
                     /pseudo
     CDS             608..799
                     /locus_tag="VFL49_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(818..894)
                     /locus_tag="VFL49_03185"
     tRNA            complement(818..894)
                     /locus_tag="VFL49_03185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(858..860),aa:Arg,seq:tcg)
     gene            complement(930..2330)
                     /locus_tag="VFL49_03190"
     CDS             complement(930..2330)
                     /locus_tag="VFL49_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7911008.1"
                     /translation="MSAVVVDADEKFDTQVPLVIVGAGAAGLCAALAAKEAGIDAVVV
                     ERDAVPAGSTALSAGLIPAAGTRFQRAKGIDDNAGLFAADIRRKAKGEADRDVVGAMA
                     AGAAPLVEWLADRYGLPFEVVDNFNYPGHSAMRMHGLPGRTGRELIDRLRDAAEANDI
                     VILTRSTAERLVADSKGRIRGVEIARRDGGRESIGCLALILACNGFGGNPELVRRYIP
                     DMASALYFGHPGNRGDAVVWGDALGAQLSHLGAYQGHSSVATPHNILITWAVIMQGGI
                     QVNGEGRRFCDETRGYSEQAAEVLRQPGGFAWSVFDARIAEVARQFDDFRQAESAGAV
                     LTANTIENLASAMRVPVAVLSAEWNDVETHKTANSEDRYGRKFEVSQACNPPFHAVKV
                     TGALFHTQGGLVIDAKGRVKRRDGTGFPNLFAAGGAAAGVSGPSAAGYLSGNGLLTAT
                     VLGRLTGESAAEQIRR"
     gene            complement(2369..3268)
                     /locus_tag="VFL49_03195"
     CDS             complement(2369..3268)
                     /locus_tag="VFL49_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004669941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7911009.1"
                     /translation="MLDEFDGGHVAVNGTRIFARSGGAGAPLLLLHGFPETHLMWRSV
                     VPVLSDRFKVVCADLRGFGQSDCPPSQRGHASYSKRTMASDMVALMERLGHRTFFVAG
                     HDRGGRVAYRLALDQPERVEGLAVLDIIPTAVAWDEADTEFALGYWPWSLLAQASPLP
                     EKILSTAAEEIVDAALGGWGSQAATFPPDVRQAYIAALRDPGHARAICEEYRAAASID
                     REHDQNDIANGRKIKCKVLALWGAGGPVDTWYEGLGGPIKIWRRFADDVEGQSVRGGH
                     FFPEQNPVETGHVLAKFFTRLRH"
BASE COUNT          667 a         1124 c          986 g          648 t
ORIGIN      
        1 cgccattgac ctggcgcgga aaatgcccgc ttcagacacc gcgcttgttg cgacaacgaa
       61 cgtcttgctg gcacgcaagg atgttcaccc ggcgatcgtc gatcttctgg cgcaaacaac
      121 cttgcaggcg cacagcacgc ctgggctttt tcgaaaggtc ggcgaatttc ccaccgagac
      181 cgatccggaa tatccaatgt cgcaaggcgc ccgcgatttc tacagaaatg gcccttcgtt
      241 cctgaaccgg tacctgccct tctggatgac caactatgcg caacgttttc tcgcagtgct
      301 ggtggcagtc atcgcaatcg ttgttccggt gtttaactat gcgccgaagc tgtttctctg
      361 gctcattcgc gatcgtgtgc gcaggctgta tcgccggctc aggctggtcg ataaagctct
      421 gctgtcaccg cccaactccg ctcagatcga ggcgctcaat gccgaactgg aaaatattgc
      481 aagagcggcc aacatcgtcc cgatgcatag ttccgaactc ttttttgaac tgagagctca
      541 tattgatcgc acccgacgtc atctggcgtc tgccgacgcg cataacggct cagccaaggt
      601 tgcctaaggc cgcattggcc attcggcgcg gatgatcaag ctcggcatcg tgttcgccgg
      661 tcaaaatgtc ggtatcaagc aggtcagcga gcgcatctgg ctcgtcagct tcatggatta
      721 cgatctggga tacttcgacg acgagacctg cagactcgag ccgctcgata acctgttcgg
      781 tccaacagtg ttaacgtaac ctatgtgcgg atatgagtgg cgcgccggaa gggattcgaa
      841 cccctgaccc ccagattcga agtctggtgc tctatccagc tgagctaccg gcgccttgag
      901 atgtcttgtg ccgcccggag gcgacgacat caacgccgta tttgctccgc cgcgctttct
      961 cccgtcaagc gcccgagaac tgtggcggtc aaaaggccat tgcccgacag atatcccgcc
     1021 gcgctcgggc ccgatacacc ggccgccgcg cctccggcgg caaacaggtt gggaaatccc
     1081 gttccgtccc tgcgcttgac ccggccttta gcatcgatca caagcccgcc ctgcgtatga
     1141 aacagcgcgc cggtcacctt gaccgcgtgg aacggcggat tgcaggcctg gctcacttcg
     1201 aatttgcgcc cgtagcgatc ctcgctgttg gccgttttat gagtctccac atcgttccat
     1261 tcagccgaaa gaactgcaac aggcacacgc attgcgctcg ccagattttc aattgtgttc
     1321 gcggtgagaa cagcaccggc gctttcggcc tgacggaaat cgtcgaactg ccgcgcaacc
     1381 tccgcaatgc gcgcgtcgaa caccgaccag gcaaatccac cgggctgccg aagaacttcg
     1441 gctgcctgct ccgagtagcc gcgcgtctca tcgcaaaagc gccggccttc gccgttgacc
     1501 tggatgccgc cctgcatgat caccgcccag gtgatcagga tattgtgcgg cgtcgcgacc
     1561 gagctgtgcc cctgataggc gccaagatgc gaaagctgcg cgccgagcgc atcgccccag
     1621 acgacggcgt cgccccggtt gccgggatgg ccgaaataga gcgcgcttgc catgtcagga
     1681 atgtatcggc ggacgagctc gggatttccg ccaaagccat tgcaggcgag aatcagcgcc
     1741 aaacagccga tgctttcgcg gccgccgtcg cggcgagcga tctccacgcc gcgaatacgg
     1801 cctttggaat cggcaacaag acgctcggcc gtgctcctgg tgagaatgac gatatcgttc
     1861 gcttccgcgg cgtcgcgcaa ccgatcaatc agctcgcggc cggtgcggcc gggcaagccg
     1921 tgcatgcgca tggccgaatg accgggataa ttgaagttgt cgaccacctc gaaaggcagg
     1981 ccgtaacggt ccgcgagcca ctcgaccagc ggtgccgcgc cggccgccat ggcgccgacg
     2041 acatcacgat cggcctcgcc tttcgccttg cgccggatgt ccgcggcgaa caggcccgca
     2101 ttgtcatcga tgcccttggc acgctggaag cgcgtgccgg ccgccggaat gagcccggcc
     2161 gagagcgcag tcgagcccgc gggcaccgcg tcgcgctcga cgaccaccgc gtcaatcccg
     2221 gcctccttcg ccgccagagc cgcgcagagt cccgcggcgc cggcaccgac aatgaccagc
     2281 ggcacctgcg tgtcgaattt ttcatcggca tcgacgacaa cggcactcat tttgggccta
     2341 tgccgcgcag gagctttccg ggcgcacgct aatgtcgaag cctcgtgaag aacttggcca
     2401 acacatgccc ggtttctacg gggttctgct ccgggaagaa atggccacca cggacggatt
     2461 gcccctcaac gtcgtcggcg aagcggcgcc agatttttat cggcccgccg agaccctcgt
     2521 accaggtgtc gacaggtccg ccggcgcccc atagcgccag aactttgcac tttatcttcc
     2581 gcccattggc gatgtcgttc tgatcgtgct cccggtcgat ggacgcggct gcacgatatt
     2641 cttcgcaaat ggcgcgagcg tgcccggggt ctcgcagggc cgcaatgtag gcttgccgaa
     2701 cgtcaggcgg gaatgtcgcg gcctgcgaac cccagccgcc aagagccgca tcgacaattt
     2761 cctcggccgc cgtagacaga atcttttcag gcaatggaga cgcctgcgcc agcagcgacc
     2821 atggccagta cccaagcgcg aattcggtgt ctgcttcgtc ccatgctacc gccgtcggga
     2881 tgatgtcgag gaccgccaga ccttccacgc gctcaggctg atccaatgcc aatcggtacg
     2941 ccactcttcc gccccgatca tggccggcga cgaaaaatgt cctgtggcca agccgctcca
     3001 tcaacgcgac catgtcactt gccatcgttc gctttgaata gcttgcgtgg ccgcgttgcg
     3061 aaggcgggca atcgctttgc ccgaagccgc gcaagtccgc gcagaccacc ttgaaccggt
     3121 cggatagcac cggaacgacc gatctccaca tcaaatgggt ctccgggaag ccgtgcagca
     3181 gcagaagcgg tgcaccggcg ccgcccgatc gggcaaaaat gcgggtgccg ttgaccgcca
     3241 catgaccacc gtcgaattcg tccagcatcg aattccctcc gccgcgtccg cacacggcaa
     3301 aggcataaac tgacagctca gtattgggtt gcggcacgcc gacgttttgt tatgcggtcg
     3361 ctttcgacaa tggcagcgtg aagaaaaaca ttaccccgcg gccggggcga ttttcagccc
     3421 atatc
//
LOCUS       DASSRL010000153         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_21618, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000153 DASSRL010000000
VERSION     DASSRL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21618"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VFL49_03200"
     CDS             complement(<1..1072)
                     /locus_tag="VFL49_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HET7911010.1"
                     /translation="MAKSIHPVDERLPFAELVPLGLQHVLVMYAGAVAVPLIIGRALK
                     LPAEQIAILVNADLFACGIVTIIQSLGIGPFGIRLPIMMGVTFASVSPMLAMANNPDL
                     GLAGIFGSVIAAGVFGLIVAPFMSSLLRFFPPVVTGTIIAVIGISLMRIGVNWAAGAP
                     APTLPGYGDPINLLVAAFVLAVILLIAKFAKGFLGNIAVLVGIIAGFIVAWIIGKVSF
                     TAFNNAAWVDIIYPFQLAAPKFDLFAVITMCLVMLVVMVESTGMFLAVGEMTDRKIEQ
                     PDLTRGLLTDGLGTLIGGIFNTFPYTSFSQNVGLVGVTGIKSRWVTVVGGVILLILGL
                     IPKLGALVTAVPLFVLGGAGIVM"
     gene            complement(1154..2050)
                     /gene="uraD"
                     /locus_tag="VFL49_03205"
     CDS             complement(1154..2050)
                     /gene="uraD"
                     /locus_tag="VFL49_03205"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET7911011.1"
                     /translation="MTAQAPISLERLNAARQEDFVAALGAIYEHSPWVAEVALARRPF
                     ASLADLHECMSAAVTGADHARQLALINHHPDLAGKAARAGSLTADSKAEQHGAGLDAL
                     SEREFNEFQRLNDAYRRKFGFPFVICVLRHTKQSVLRQLDLRGKNDTPTERANALKEV
                     IRIAALRLDRRVTAQDRLKIHGRLSTHVLDTCHGRPAQGVSLELRELSEPGSGRLMTA
                     AVTNADGRTDRALISDRPVPIGSYELRFAVGDYFRRSGIVLPDPPFLDIVSVQFSIAE
                     PEGHYHVPLLLTPWSYSTYRGS"
     gene            complement(2047..>2606)
                     /locus_tag="VFL49_03210"
     CDS             complement(2047..>2606)
                     /locus_tag="VFL49_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HET7911012.1"
                     /translation="HGLKWIEYKDVPEDEERSHMAEAIRIHQQATGARPLGWYTGRSS
                     VNTLRAVLDDGGFRYSSDSYADDLPYWVKGSKGPHLIIPYTLDVNDMRFINAQGFGTS
                     DEFFVYMRDTFDTLYAEGGAAPKMMSVGLHCRLAGRPGRVAAITRFLDHVTKYDRVWI
                     ATRLEIAEHWHREHSALQKTAPVVA"
BASE COUNT          578 a          785 c          779 g          464 t
ORIGIN      
        1 acatgacgat gccggcgccg ccaagcacaa acagcggcac cgcggtgacc aacgccccga
       61 gcttggggat gagcccgagt atgagcagga taacgccgcc aaccactgtg acccaacggc
      121 tcttgatgcc ggtcacgccg accagaccga cgttctgcga aaatgacgtg taggggaagg
      181 tattgaaaat tcccccgatc agcgtgccga gaccgtccgt caagaggccg cgcgtaaggt
      241 ccggctgctc gatcttccgg tcggtcatct cgccgaccgc caggaacatg ccggtcgatt
      301 ccaccatcac cacgagcatt accagacaca tcgtgatcac ggcgaagaga tcgaatttcg
      361 gtgcggccaa ctggaacgga taaataatat cgacccaggc cgcgttgtta aacgccgtga
      421 agctcacctt accgatgatc caagcgacga taaagccggc aataatgcca accagcaccg
      481 cgatgttgcc gagaaagcct ttcgcaaact tggcgatcag caggatcacg gccagcacga
      541 atgccgcaac gagcaaattg attggatcac cgtagccggg aagcgtgggt gccggcgcgc
      601 cggctgccca attgacgccg atgcgcatca gcgagattcc gatcacggcg ataatcgtgc
      661 ccgtcaccac cggcgggaaa aagcgcaaca gcgagctcat gaatggcgcg acgatcaggc
      721 caaagacgcc ggcggcaatg accgagccga agatgcccgc gaggccgaga tccggattgt
      781 tcgccatcgc cagcatcggt gagacggacg caaatgtaac gcccatcatg ataggcaagc
      841 gaattccgaa tgggccgatg ccgagggatt ggatgattgt aacgataccg caggcaaaca
      901 aatcagcgtt gaccagaatt gcaatctgtt cggcgggcag cttcagcgcg cggccaatga
      961 tcaacggcac cgcgacagcg ccagcataca tcaccagcac gtgttgcagt cccagcggaa
     1021 cgagttcggc gaacggcaac cgctcgtcta caggatggat tgattttgcc acgatagccc
     1081 ccctttatta cccccggcac cggtgatgaa taccggagcc gccagcggat tatagcgggg
     1141 tgaacgtcgc acgctagctt cctcgatacg ttgaataact ccacggggtc agaagcagcg
     1201 gcacgtggta gtggccctcg ggttccgcga tcgagaactg cactgagacg atatcgagaa
     1261 atggcggatc tggcaggacg atgccgctcc gcctgaagta gtcgccaact gcaaaccgca
     1321 gttcgtaact gccgatcgga accggccggt cactgatcag cgcccggtcg gtgcggccat
     1381 ccgcattcgt cactgccgcg gtcatcagac ggccgctgcc cggttccgac aattcgcgga
     1441 gttctaggga aaccccctgt gccgggcgcc cgtgacaggt atcaagtacg tgggtcgata
     1501 accgtccatg gatcttaagc cggtcctggg cggtgacgcg ccgatcaagc cgcagagcgg
     1561 cgatgcggat aacctctttc aaggcatttg cccgttcggt gggcgtatcg tttttgccgc
     1621 gcagatcgag ctggcgcaaa accgattgct tggtgtggcg aagcacgcag ataacgaaag
     1681 gaaagccgaa cttcctgcga taggcgtcat tgaggcgctg aaattcgttg aactcgcgct
     1741 cgctcaacgc atcgagtccc gcgccatgct gttccgcctt cgaatcggcg gtcagtgatc
     1801 cggcccgcgc agccttgcct gcgagatcgg ggtggtgatt gatcagcgca agctggcgtg
     1861 cgtgatcggc accggtcacc gcggcgctca tgcattcatg caggtccgca agcgaggcga
     1921 aagggcgcct cgccagcgca acctcggcaa cccatggcga atgttcgtaa atggcgccga
     1981 gcgccgcgac gaagtcttcc tgcctggccg cattaagtct ttcgagagag ataggcgcct
     2041 gtgctgtcat gccaccaccg gagcggtctt ctggagcgcc gaatgctcac gatgccagtg
     2101 ctcggcaatc tcgagacgag tcgcaatcca aactcggtca tatttggtga catggtcgag
     2161 aaaacgcgtg atcgcagcaa cccgtcccgg gcgccccgcg agacggcaat gcaggccgac
     2221 tgacatcatc ttcggtgcgg ctccgccttc ggcgtagagc gtatcgaacg tgtcgcgcat
     2281 gtagacgaag aactcatcac tcgtgccgaa gccctgcgca ttaataaagc gcatatcgtt
     2341 gacgtcgagg gtatagggga tgatcagatg cggacccttc gaccctttca cccaatacgg
     2401 caggtcgtca gcgtacgaat ccgatgagta gcggaagccg ccgtcatcga gcacggccct
     2461 tagcgtattg acggagcttc ggcctgtata ccagccgagc gggcgcgcgc cggtcgcttg
     2521 ctgatgaatg cggatagcct ccgccatgtg cgaccgctct tcgtcctcgg gcacgtcttt
     2581 gtactcgatc catttgagac cgtggc
//
LOCUS       DASSRL010000154         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1386476, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000154 DASSRL010000000
VERSION     DASSRL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386476"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFL49_03215"
     CDS             <1..573
                     /locus_tag="VFL49_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911013.1"
                     /translation="GAWIVMQNTEQYVWSGVALSIHNPFPDNPLVLGPLSVSWNRVYV
                     FVIALALIFATYYLVNRTRLGKAMRATFQDRDTAALMGIRIDRIHTATFALGSGLAAA
                     AGALLGPVFVVAPTMGDLAASKAFAIVILGGLGNITGATLGGFILAFVEEIGAGYISS
                     GYRDAMGFLLIILILLARPTGLFAQRERIG"
     gene            570..1604
                     /locus_tag="VFL49_03220"
     CDS             570..1604
                     /locus_tag="VFL49_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911014.1"
                     /translation="MRRYLGWPLVVFMAIFPLWLNDFYLLHILITTGIFIIAAMSLNL
                     LLGYTGQLSLGHVAFFGIGAYTTALMSLGFNVHVLPDFTVGLAPKPVWFAILTAVIVA
                     GLTGYAIGKISFRVRGAYFVIVSISFAEVVRLVAVNWVELTQGPMALNNIPALTLGAG
                     GLELSFLRKPAFYLLVLSTIVICYVLIARLVNSRAGRAMIALRENEPLATSIGIDVTH
                     YLVIATVVSAAMAGMAGALYAHYIRIVDPDIFLFIYTVTMVIMVITGGKGTLVGPIVG
                     GALFGFIPEILRTMEIVPEIQWIIYGVLMVLVVYFLPRGIVPALETYFSRRKRRPVER
                     SASAAAEPEK"
     gene            1601..2386
                     /locus_tag="VFL49_03225"
     CDS             1601..2386
                     /locus_tag="VFL49_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911015.1"
                     /translation="MSVTPETILSIDHVAVHFGGLIAISDMTFGVRRGEIVGLIGPNG
                     AGKTTAFNVMTGFQRPTNGDVHYRGHVLTGMKPHEIAKLGLVRTFQRTSVFRERSVFQ
                     NVLTGLHCSGRSSLWDSFLQSRREITSERELSDSAHEILRFVGLESRAGEQAGILPYG
                     EQRLVGVALALAAKPTMLLLDEPVSGMNQTETARFVRLVEKISASGVTILLVEHDMPM
                     VMEVCERIVVLNNGSIIAEGTPAEIQNDPEVIRAYLGEGRKRA"
     gene            2379..3083
                     /locus_tag="VFL49_03230"
     CDS             2379..3083
                     /locus_tag="VFL49_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911016.1"
                     /translation="MLELRDVVCRYGEVTAVKGLSLRVGRGQLVALIGTNGAGKTTTL
                     RTISGLLHPARGSITFDGIDITRASARKILSLGIAHCPEGRHVFPYMTVTENLEMGCY
                     LRTDRQEMAQDMDRVFDQFPRLAERRRQQAGTLSGGEQQMLAIARALMSRPKLIMFDE
                     PSLGLAPNIVERTFEIIIGIRAAGTTVLMVEQNALAALEMCDHAYLLESGSLALSGGG
                     RELVDNPHVRSAYLAG"
     gene            complement(3068..3700)
                     /locus_tag="VFL49_03235"
     CDS             complement(3068..3700)
                     /locus_tag="VFL49_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7911017.1"
                     /translation="MTVEAALGSFIGRRRSIRRYRAGPIARSVVERLLLAATQAPSAH
                     NRQPWRFAVIDDYAAKATLAAAMGQRLRQDRTSDGDDPQVVEADVSRSFARITEAPVV
                     IVVCADVRDMDQYADDRRRQSEFLMAVQSAAMAAQNLLLAAEQERLGACVMCAPLFCP
                     DAVMDALDLPQGWQAQMLITIGQPDSAGKNRPRLSLAEVTTWFSPDQPAK"
     regulatory      3888..3974
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      3976..4043
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT          789 a         1157 c         1234 g          945 t
ORIGIN      
        1 ggcgcttgga ttgttatgca gaataccgag cagtatgtgt ggtccggggt cgcactgtcg
       61 atccacaatc cgtttcccga taacccgctg gtgcttggcc cgctatccgt gtcctggaac
      121 cgcgtctacg tattcgttat cgcactcgcc ttgatcttcg cgacttacta tctcgtcaat
      181 cgcacacggc tcggtaaagc gatgcgtgcg acctttcagg acagggacac cgcagcgctg
      241 atgggaatcc gcatcgaccg tatccacacc gcaacctttg cgttgggctc aggtctcgcg
      301 gcagcggccg gcgcactgct cggtcccgtg tttgttgtcg cgccgacgat gggtgatctc
      361 gccgcttcca aggcctttgc gatagtcatc cttggtgggc tcggaaacat caccggcgca
      421 accctgggcg gctttatcct cgcttttgtc gaggaaatcg gtgcggggta catctcttcc
      481 ggttaccgcg acgccatggg ttttctgctg atcatcctga tcctgctggc gcgcccgacg
      541 ggccttttcg cgcagaggga gcggatcggg tgaggcgata tctcggctgg cctctcgtcg
      601 tgtttatggc gatcttcccg ctgtggctga atgacttcta tttgctccat atcctgatta
      661 cgaccggaat tttcatcatt gcggcgatga gcctcaatct gcttctcggc tataccggtc
      721 agttatcgct cggccatgtc gcattctttg gaatcggcgc gtacacgacc gcgctgatgt
      781 cgcttggatt taatgtgcac gtgctccctg acttcaccgt gggccttgct ccgaagccag
      841 tctggttcgc cattctgacc gcggtcatcg ttgcggggct gaccggctac gcaatcggaa
      901 aaatttcgtt ccgcgtgcgc ggagcttatt ttgttattgt ttcgatcagc ttcgccgaag
      961 ttgtccggct ggttgccgtt aattgggtcg aactgacaca aggaccgatg gcgctcaaca
     1021 acatcccggc gcttacactc ggcgctggcg gattagagct gtcgttcctg cgcaaacccg
     1081 cattttatct tctcgtgctg tcgacaatcg tgatttgcta tgtcctcatc gcgcggctgg
     1141 tgaactcccg tgccgggcgg gcgatgattg ccctgcgcga aaacgaaccg cttgccacgt
     1201 cgatcggcat agatgttacc cattacctgg taattgcgac tgttgtttcg gcggcgatgg
     1261 caggaatggc gggcgctctc tatgcccact atatccgcat cgtcgatccc gacatctttc
     1321 tgttcatcta cacggtgaca atggtgatca tggtgatcac cggcggcaag ggtactttgg
     1381 tgggaccgat tgtcggcggg gcgctgtttg gattcattcc ggaaatattg cgcaccatgg
     1441 agatcgtgcc ggaaatacag tggattattt acggcgtcct gatggtgctg gttgtctatt
     1501 ttctaccgcg tggaattgtg cccgcgctgg aaacctactt ttcccggcgg aagagaaggc
     1561 cggtcgagag gtcagcgtcc gctgccgcgg agccggagaa atgagcgtaa caccggaaac
     1621 tatcctgtcg attgatcatg tcgcagttca ttttggcggc cttatcgcca tttccgatat
     1681 gacattcggc gtcaggcgcg gcgagattgt cggactgatc ggcccgaatg gtgcgggcaa
     1741 gacgaccgca ttcaatgtca tgacagggtt tcagcggccg accaacggcg atgtgcatta
     1801 tcgcggccac gtgctcactg gaatgaagcc gcatgaaatc gccaaactcg ggctggtgcg
     1861 gaccttccag cggacaagcg tattcaggga gcgaagcgta ttccagaatg tgcttaccgg
     1921 attgcactgc agcggccgtt cgtcgctatg ggattcgttt ctgcaatcac ggcgcgagat
     1981 cacgtccgag cgggagcttt ccgactccgc gcacgaaatt ttgcggtttg tcggactcga
     2041 aagtcgtgct ggagagcagg cgggcatctt gccgtatggc gagcagcgcc tcgttggcgt
     2101 tgcgctcgcg cttgctgcaa aaccgactat gctgctgctc gacgagccag tctccgggat
     2161 gaaccagacg gagaccgcgc gcttcgtcag gctggtcgaa aagatcagcg cctctggcgt
     2221 cacgattctg ttggtcgagc atgatatgcc aatggtgatg gaagtctgcg aacgcatcgt
     2281 cgtgctcaac aatgggagca tcattgccga aggcacaccg gcggaaattc agaatgatcc
     2341 cgaagtaatc cgcgcctatt tgggagaggg ccggaaacgt gcttgagctt cgcgatgttg
     2401 tctgccggta cggcgaggtg actgcagtaa aggggctgtc actgcgggtt gggcgcggtc
     2461 agctggttgc gcttatcggc acgaatggcg ccggcaaaac cacgacgctg cgcacgatat
     2521 cggggctgct gcatccggcc agaggcagta ttacctttga tggcatcgac attacacgcg
     2581 cttcggctcg taaaattctg tcactcggta tcgcgcattg cccggaaggg cgccacgttt
     2641 ttccctacat gacagtgaca gaaaatctcg aaatgggatg ctatctgcgc accgaccgcc
     2701 aggaaatggc gcaagatatg gatcgcgtgt ttgaccaatt tccccgtctg gcggaacgcc
     2761 ggcggcagca ggccgggaca ctgtctggcg gcgaacagca gatgcttgcc atcgctcgtg
     2821 cactgatgtc ccggccaaag ctcattatgt ttgacgagcc ttcactggga cttgcaccta
     2881 atatcgtcga acgcacgttt gagattatca tcggcattcg cgccgcgggc acgaccgtgc
     2941 tgatggtcga gcaaaacgcg ttggcggcgc tcgagatgtg cgatcacgcg tacctcctgg
     3001 agtcgggctc cctggcgttg tctggaggtg ggcgcgaact ggtcgacaac ccacatgtcc
     3061 gcagcgccta tttggccggc tgatctggcg agaaccaggt ggtgacttcc gcgagcgaca
     3121 agcgcggacg atttttgccg gcgctgtcgg gttgtccaat cgtaataagc atctgggctt
     3181 gccagccctg tggcaggtcc aatgcatcca taacggcatc cggacagaaa agcggggcgc
     3241 acatcacgca ggcacccagt cgttcctgtt cggccgcaag cagaagattt tgtgcagcca
     3301 tggcagcgct ctggactgcc atgagaaatt ccgactgccg cctgcgatcg tcggcatact
     3361 ggtccatgtc tctgacatct gcgcaaacca ctataacgac cggcgcttcg gtgatcctcg
     3421 caaaggagcg ggacacgtct gcctcgacga cctgcgggtc atctccatcg ctggtgcggt
     3481 cttggcgcag acgttgtccc attgccgccg ccagcgttgc ctttgcagcg tagtcatcga
     3541 tgactgcaaa acgccatggc tgccggttgt gagcagacgg cgcctgcgtc gcggccagca
     3601 acaaacgctc gactaccgaa cgggcaattg gacccgcccg gtaccggcgt attgaccggc
     3661 gcctgccgat aaaggatccg agtgctgcct cgaccgtcat tgcagtctcg tgcgcaaaaa
     3721 gagcaggagc ggtaagggga gatcatggca gcggcgcgat gcgttgtcat gataaggcat
     3781 tgtattcatt cccggctcgc caaaaatgtg ctgtgagagg ggaggactct ttgctattgt
     3841 tactatgttt gtgaccttgg taaaggagca atccctcact tgataatgtt ccccttgcgg
     3901 gagagatcgg tccgaccgac gccgaaggag caaccgcacc ggaaactctc aggcagcaga
     3961 atcgcaaggg ttgtgacact ctggagagag gcgctaacgc gtccgccgac gggataacac
     4021 tctcaggcat caggacagag ggggcaccac attctcggcc agcccggccg aattaggggt
     4081 gcctcttatg cttatttttc tcagctggaa tggttgtgct catcc
//
LOCUS       DASSRL010000155         4554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_914205, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000155 DASSRL010000000
VERSION     DASSRL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914205"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFL49_03240"
     CDS             <1..507
                     /locus_tag="VFL49_03240"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197126.1"
                     /note="methionine--tRNA ligase; MetRS; adds methionine to
                     tRNA(Met) with cleavage of ATP to AMP and diphosphate;
                     some MetRS enzymes form dimers depending on a C-terminal
                     domain that is also found in other proteins such as
                     Trbp111 in Aquifex aeolicus and the cold-shock protein
                     CsaA from Bacillus subtilis while others do not; four
                     subfamilies exist based on sequence motifs and zinc
                     content; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7911018.1"
                     /translation="NADLANDLGNLAQRSLSMIGKALSGKLPKPGAYSDADKTILGAA
                     DAMIVTAREHMKTQQLHQVLNCIWAVVADANRYFASEAPWALAKTNPERQGTVLYVTA
                     EILRQAGILAQPFVPSSAAKLLDLLAVPAAERAFSALTPERRIKAGVTLPPPSPVFPR
                     YVEAEEKA"
     gene            510..1304
                     /locus_tag="VFL49_03245"
     CDS             510..1304
                     /locus_tag="VFL49_03245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7911019.1"
                     /translation="MMVDSHCHLDFPDFAAELDAVVGRARAAGIGRLVTISTRVKRHA
                     QVLAIAERFPEVFCSVGTHPNHAHEEPDIDATALIAIAKHPKVVAIGEAGLDYFRKGS
                     PREVQAAGLRQHIAAARKTGLPLVIHSRDCDADMAQILTQESGKGAFPAILHCFTGGR
                     DLAFAAIELGHYVSFTGILTFKNSDALRAIAAALPADRIMVETDAPYLAPLPYRGKRN
                     EPAYVVETAKVLADARGVSADEITRQTTDNFFRLFNKVPRVLADAA"
     gene            1301..2098
                     /locus_tag="VFL49_03250"
     CDS             1301..2098
                     /locus_tag="VFL49_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7911020.1"
                     /translation="MSLTFTILGCGSSMGVPRVALGWGSCDPNNPKNRRRRCSLLVER
                     TNAHGGRTRVLVDCSPDLRMQLLDAEVDWLDAVLITHEHADHTHGIDDLRPLFVHKRR
                     RIDVYMDEPTSQAMTARFAYCFATPPGSEYPPIATERRLVAGRAVTIDGQGGPIEALP
                     VLQQHGDIPSLGFRFNNVAYSADIKGLAAASVAALDGLEVWIVDALRKSPHPSHFNLD
                     EALDWIGRVKPKRAILTNLHTDMDYATLRAELPPGVEPGYDRMSFVE"
     gene            2316..3119
                     /gene="mazG"
                     /locus_tag="VFL49_03255"
     CDS             2316..3119
                     /gene="mazG"
                     /locus_tag="VFL49_03255"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET7911021.1"
                     /translation="MKPSRDISRLLEIMAALRTPGTGCPWDLEQNFRTIAPYTLEEAY
                     KVADAIARDDLPDLQDELGDLLLQVVFHARMAQEQDAFDFGDVVQALTAKLIRRHPHV
                     FADADGKTADAVKGLWERIKAEEKAARGEKGGASDGALAGVPVGLPALTRALKLQDKA
                     GRVGFDWNDPRAVLAKIREEADEIEAELDRADGNRMAAEVGDLLFAVVNLARHLGADP
                     EGVLRETNLKFERRFASIERALAARGKTPRQATLAEMDALWDEAKAAEK"
     gene            complement(3122..4405)
                     /gene="hflX"
                     /locus_tag="VFL49_03260"
     CDS             complement(3122..4405)
                     /gene="hflX"
                     /locus_tag="VFL49_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7911022.1"
                     /translation="MEPWIKAAPRRNGVGAQASARTPEARLEEAVGLARAIDLTIVQA
                     GLVTLSDIRPATYIGKGKVDEIAGMVKSLSASIVVMDCPISPVQQRNLERAWSAKVID
                     RTGLILEIFGRRARTREGALQVELAHLTYQKSRLVRSWTHLERQRGGFGFLGGPGETQ
                     IEADRRVIEERIARIEADLDKVKRTRKLHRDSRKRVPYPIVALVGYTNAGKSTLFNRL
                     TAATVLSADMLFATLDPTLRAVELPRGAKVILSDTVGFISDLPTMLVAAFRATLEEVI
                     EADVILHVRDVSHGDSEAQLYDVEKVLDELGIAASDRRLIEVWNKIDCLDTEARARVL
                     NLADRRAAGHRPVAVSALTGEGMDGLLTAIDARLGESRQTIEVSVDPADGAGLSWLYR
                     HSEVLARDMNDDGRLTVTVRADADNAARVRAKFNS"
BASE COUNT          842 a         1468 c         1391 g          853 t
ORIGIN      
        1 aatgcagatc tggcgaacga tctcggcaat ctggcgcagc ggtcgctctc aatgatcggc
       61 aaggcgctga gcggcaagct gccgaagccg ggggcctaca gcgatgccga caagacgatt
      121 ctcggcgccg cagatgcgat gatcgtcacc gcgcgcgagc acatgaagac ccagcagttg
      181 caccaggtgc tcaattgcat ctgggcagtg gttgccgatg ccaaccgcta ttttgcgtcg
      241 gaagcgccct gggcgctggc caaaaccaac cctgagcgcc agggtacggt gctctatgtc
      301 acggccgaaa tccttcgcca ggccggcatc ctcgcccagc ccttcgtgcc ctcatcggcc
      361 gccaaattgc tcgacttgct cgcggtgccg gctgccgagc gcgcgttttc ggcactcacg
      421 ccggagcggc gcatcaaggc cggggtcaca ctgccgccgc cttcaccggt ttttccccgc
      481 tacgtggaag cggaagagaa agcatgacca tgatggtcga cagtcactgc cacctcgatt
      541 ttccggattt tgccgccgag ctcgacgctg tggttggccg cgcccgggcc gccggtatcg
      601 gccgcctggt gacgatctcc acgcgcgtca agcgccacgc gcaggtgctg gcgatcgccg
      661 aaaggtttcc cgaagtcttc tgctccgtcg gcacgcaccc gaaccacgcg cacgaggagc
      721 cggacatcga cgcgacggcg cttatagcga tcgcaaaaca tccgaaggtc gtcgcaatcg
      781 gcgaggcggg gctcgactat ttccgcaagg gcagcccgcg tgaggtgcag gctgccggtc
      841 tgcggcagca tattgccgcc gcccgcaaaa ccgggctgcc gctggtcatt cattcacgcg
      901 actgcgatgc cgacatggcg caaattctga cgcaggagag cgggaagggc gccttcccgg
      961 ccatcctgca ttgctttacc gggggccgcg acctggcatt cgctgccatc gaactgggcc
     1021 actacgtctc ctttacgggc atcctgacct tcaaaaattc cgatgcgctg cgcgcgattg
     1081 ccgcggcgtt gccggccgac cgcatcatgg tggagacgga tgcgccctat ctggcgccgc
     1141 tgccgtatcg cggaaaacgt aatgaacccg catacgtggt cgaaaccgct aaagttttag
     1201 ctgatgcgcg cggggtatcg gccgacgaga ttacacggca aacgacggac aatttcttcc
     1261 ggcttttcaa taaggttccg cgcgtacttg cggacgccgc atgagcttga cctttacgat
     1321 cctcggctgc ggctcatcga tgggcgtgcc gcgcgtggca cttggctggg gctcgtgcga
     1381 tccgaacaat ccgaagaacc gccgccggcg ttgctcgctg ctggtcgagc gtaccaatgc
     1441 ccatggcggc cgcacccgcg tcctggtcga ttgctcgcca gatctgcgca tgcaactttt
     1501 ggacgccgag gtcgactggc ttgatgccgt gttgatcacg cacgagcatg ccgaccatac
     1561 ccatggcatt gatgatttac gcccattgtt cgtgcacaag cgccgccgga tcgacgtcta
     1621 catggatgag cccacgtctc aagctatgac cgcccgcttc gcctattgct tcgcgacgcc
     1681 tccaggcagc gaatacccgc cgatcgccac cgaaagacgc ttggtggccg gtcgcgcggt
     1741 cacaatcgat gggcagggtg gaccaataga agcccttccg gtgctgcagc agcatggtga
     1801 catcccatcc ttgggcttcc ggtttaacaa tgtggcctat tcggctgata tcaaaggcct
     1861 ggctgccgca agcgtcgccg cgcttgacgg cctcgaggtc tggattgtcg atgcgctccg
     1921 caaaagcccg catcccagtc acttcaatct cgacgaggcc ctggactgga tcggcagggt
     1981 caagccaaag cgggcgatcc tgaccaatct gcacaccgac atggactacg cgacgttgcg
     2041 cgccgaactg ccgcccggcg tcgaacccgg ctacgaccgg atgtcgttcg tcgaatgaag
     2101 cgtcccggct acgatcgcgc gcgcggcgcc gggaaaggtc gagttttatc gattggtgtc
     2161 cggctcggtt catttagcct tggtattacg ggcctatata aaatattcca taatatgtct
     2221 tatgcgaatc cgctatatgc ggtcgcggct cggcgggggc tttgctacgg tcccgtgaac
     2281 ggtccgcgcc aaacgccagg cacacgtcga tccccgtgaa gccatcccga gacatttcgc
     2341 gcctgctcga gatcatggcc gcgctccgta cgcctggcac cggctgcccg tgggatttgg
     2401 agcagaattt cagaacgatc gctccctata cgctcgagga agcctacaag gtggccgacg
     2461 ccatcgcgcg tgacgacctg ccggacctgc aagacgaact gggcgatctg ctgctgcaag
     2521 tcgtattcca tgcccggatg gcacaggagc aagacgcctt cgatttcggt gacgtcgtgc
     2581 aggcgttgac cgccaagctg atacgacggc atccccatgt ctttgcggac gccgacggca
     2641 agaccgcgga tgccgttaag ggtttgtggg agcgcatcaa ggccgaggag aaggctgcgc
     2701 gcggcgagaa gggcggtgcc tcagatggcg ccctggccgg cgttccggtc ggcctgcctg
     2761 cgttaacccg tgccctgaag ctgcaggaca aagccggacg cgtgggcttc gactggaacg
     2821 atccgcgggc ggtgcttgcg aagatccgcg aagaagcgga cgagatcgag gccgaactcg
     2881 accgtgccga tggcaaccga atggctgccg aagtcggcga cctgctgttt gcggtggtca
     2941 acctcgcccg tcatctgggc gcggaccctg aaggtgtcct gcgcgaaacc aacctgaagt
     3001 tcgagcggcg ctttgcatcg atcgagcgag ccctcgctgc gcgcggaaag acaccgaggc
     3061 aggcgaccct ggcggagatg gacgcccttt gggacgaagc gaaggccgcc gaaaagtaaa
     3121 ttcaggagtt aaactttgcc cgaacccgcg ccgcgttgtc ggcatcggcg cgcaccgtga
     3181 cggtcaggcg gccgtcatca ttcatgtctc ttgcgagcac ttcggagtga cggtagagcc
     3241 agctcaggcc ggcaccgtcc gcaggatcaa ccgagacctc aatcgtctgc cggctttcgc
     3301 ccaagcgtgc gtcgatggcg gtcaaaaggc cgtccatgcc ctcaccggtt aacgccgaca
     3361 cggcgaccgg acggtggcct gcggcgcggc ggtcggcgag attgagcacc ctcgcccgcg
     3421 cttctgtgtc gaggcaatca atcttgttcc agacttcgat caatcgtcgg tcgcttgccg
     3481 cgatccccag ttcgtcgaga accttttcga cgtcgtacag ctgagcttcc gaatccccgt
     3541 gcgagacatc acgcacgtgc aggatcacat ccgcctcgat gacctcttcc agcgtcgccc
     3601 ggaacgccgc caccagcatc gtcggcagat ccgagatgaa gcccaccgtg tcggatagga
     3661 tgaccttggc cccgcgcggc aattccacgg cgcgcagagt cggatcgagt gtcgcgaaca
     3721 gcatgtccgc tgagaggaca gtcgcagcag taagcctgtt gaacagcgtc gattttccag
     3781 cgtttgtata cccgaccagc gcaacaatcg gataaggcac gcgcttgcgg ctgtcgcggt
     3841 gaagcttgcg ggtgcgcttg accttgtcca gatccgcttc gatgcgcgcg atccgctcct
     3901 cgatcacgcg ccggtcggcc tcgatctggg tctcgccggg gccgcccagg aagccgaagc
     3961 cgccgcgctg acgctcaagg tgggtccacg accgcaccag cctgcttttc tgataggtca
     4021 gatgcgccag ctccacctgc aacgcccctt cccgcgtgcg tgcccgccgg ccgaaaatct
     4081 cgaggatcaa acccgttcga tcgatgactt tcgcgctcca tgccctttcg agattgcgct
     4141 gttgaaccgg agagatcggg caatccatga cgacaatcga agccgacagg cttttcacca
     4201 ttccggcgat ctcgtcgacc ttgcctttgc cgatgtaagt tgcaggccgg atatcgctga
     4261 gagtgacgag ccctgcctgc acgattgtaa gatcaattgc tcgcgcaagg ccgacagcct
     4321 cttcgagacg cgcttcgggc gtgcgcgcgc ttgcttgcgc gccaacaccg ttgcgacgag
     4381 gcgctgcctt gatccagggc tctatgacga tcgcacggcc ggtctcactg cccgacgcca
     4441 ccgggcttaa gagatcactc gcctgatcac gtcgacgtaa ttccaactaa gccttgtctc
     4501 cggccggcgg ctcgtcggcc ccttcgaaga gttggatcgg gtgtcccggc atga
//
LOCUS       DASSRL010000156         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_4352, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000156 DASSRL010000000
VERSION     DASSRL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4352"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..348
                     /gene="grxC"
                     /locus_tag="VFL49_03265"
     CDS             76..348
                     /gene="grxC"
                     /locus_tag="VFL49_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HET7911023.1"
                     /translation="MPAVEICTTRLCPHRSAAKALLTQKGVSYSEIHISRDFERREEM
                     IQRANGRMTVPQIFIGIAHIGGSHELHALERVGKLAPPLAGGKASS"
     gene            345..1220
                     /locus_tag="VFL49_03270"
     CDS             345..1220
                     /locus_tag="VFL49_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7911024.1"
                     /translation="MNGGTFKAAMIQMRSGLKPGANIDAAVRYIGEAKSSGAEYVLTP
                     EMTNILAANREQLFAVAVEEEADASLATLREVARKLGIYVHIGSLAIKISPDRAANRS
                     FLIDPKGDILARYDKIHMFDVDLAGGESYRESRNYRPGELAVLADLPWGRLGLTVCYD
                     LRFPALYRALAEAGATMLAIPSAFTKQTGEAHWHVLIRSRAIENGCFVFAAAQGGRHE
                     NGRDTFGHSLIVDPWGRIIAEGGTEPGVIVAEINPAEVANARARIPSLQHGRRFEIVE
                     PLAEPAHLHVVRGAE"
     gene            1217..1705
                     /locus_tag="VFL49_03275"
     CDS             1217..1705
                     /locus_tag="VFL49_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HET7911025.1"
                     /translation="MIRYALVCREGHQFESWFQDSAAYDKQAKRSLISCPHCGSVKVE
                     KAIMAPRLAATAKKRDKPIEAPAAAPEAAPAPAPASAPVAMISPQEQEIRTKLKELRD
                     HLIKNADNVGTKFPEEARKMHYGEIEHRSIYGVASPEEAKELSEEGIEFHPLPSLPDE
                     RN"
     gene            complement(1702..>2312)
                     /locus_tag="VFL49_03280"
     CDS             complement(1702..>2312)
                     /locus_tag="VFL49_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HET7911026.1"
                     /translation="SFVVAYAYIKTEPVQVALFGLIFLGDRVTPLAAAAILIATAGVI
                     VISLKPSTGGTRTMRSTLIGLAGGTMFALSAISYRGAILSLGLSDFVLAATFTVTVGL
                     VIQAALLSAYLALRDPKVLHAIARAWKPSLFAGFMGAFASEFWFLAFAITTAANVRTL
                     ALVEVLFAQGVTRFVFKQPTTQREGIGIVLVVIGVALLVWAY"
BASE COUNT          497 a          742 c          701 g          372 t
ORIGIN      
        1 atgtactggt ctttgcgcgg gttgtggtgg cggcacgtcc tcccatataa ggaagctcaa
       61 atgcccgaaa acaccatgcc tgccgttgaa atctgcacca cccgcctttg tcctcaccgc
      121 tccgcggcca aggccttgct gacgcaaaag ggtgtaagct acagcgaaat ccacatcagc
      181 cgggatttcg aacggcggga agaaatgatt caacgtgcaa acggacgcat gaccgtgccg
      241 cagattttta tcggcatcgc acacatcggc ggcagccatg agctgcacgc gctcgagcgc
      301 gtgggcaagc tcgcaccgcc attggccggc ggcaaggcct cgtcatgaac ggcggcacgt
      361 tcaaggcggc gatgattcag atgcgttcgg gcctcaagcc cggtgccaat attgatgctg
      421 ctgtgcgcta catcggcgag gcgaaatctt cgggcgccga atacgtgctc acgcccgaga
      481 tgacaaacat cctcgcggcg aatcgcgagc agctcttcgc ggtggccgtc gaagaagagg
      541 ccgacgcatc gctggcgacg ctgcgcgagg tcgcacgcaa gctcggcatc tacgttcata
      601 tcggctcgct ggcgatcaag atctcgcccg accgtgccgc caaccgttcg ttcctgatcg
      661 atccgaaggg cgacatcctt gcgcgctacg acaagatcca catgttcgac gtcgaccttg
      721 cgggcggcga aagctatcgc gaatcgcgca attaccgacc gggtgagctg gcggtcctgg
      781 ccgacctgcc ctggggccgt ctcggattga ctgtttgcta cgacctgcgt tttccggcgc
      841 tttaccgcgc gttggccgaa gccggcgcaa ccatgctggc aatcccgtcg gcatttacaa
      901 agcagacggg cgaagcccat tggcatgtcc tcattcgcag ccgtgccatt gaaaatggct
      961 gctttgtttt tgcagcggcc cagggcggca gacacgagaa tggcagggat acgttcggcc
     1021 attcactgat cgttgaccct tgggggcgca tcattgccga aggtggcacc gagccggggg
     1081 tcattgtggc cgagatcaat cccgcagaag tcgccaatgc gcgtgcgcgc attccctcgc
     1141 tgcagcatgg gcggcgattt gaaatcgtcg aaccgttggc cgagccggcg cacctccacg
     1201 tcgtacgggg cgcagaatga ttcgctacgc actcgtctgc cgggagggcc accagtttga
     1261 aagctggttc caggattcgg ccgcctacga caagcaggcc aagcgtagct tgataagctg
     1321 cccgcactgc ggctcggtca aggtcgaaaa ggcgatcatg gcgccgcgcc tggccgcgac
     1381 cgcaaaaaag cgggataaac ccatcgaggc ccccgcggcg gcacccgaag ctgcgcccgc
     1441 accagcgccc gcatcggcgc ctgtcgcgat gatctcgccg caggagcagg aaatccgcac
     1501 caagctcaag gaactccgcg atcacctcat caagaacgcc gataatgtcg gcacgaaatt
     1561 tcccgaggaa gcgcgcaaga tgcactacgg cgagatcgaa catcgctcga tctacggcgt
     1621 ggcatcgccg gaagaagcga aagaactctc cgaggaaggc atcgaattcc atccgctgcc
     1681 gtcgctgccc gacgagcgca attagtacgc ccacaccaag agcgcgacgc cgatcaccac
     1741 cagcacaatg ccgatgccct cgcgctgcgt ggtcggctgc ttgaagacaa accgcgtcac
     1801 gccctgcgcg aacagcacct cgaccaacgc gagcgtgcgc acgttggccg ccgtcgtgat
     1861 ggcaaaggcg agaaaccaga attccgaggc aaacgcgccc atgaagccgg cgaacaacga
     1921 cggcttccag gcccgcgcaa tcgcgtgcag caccttgggg tcgcgcagcg cgagataggc
     1981 cgacagcaac gcggcttgaa tcaccagccc cactgtaacg gtgaacgtcg cggccaggac
     2041 gaagtccgac aggcccagcg acagaatcgc gccgcgataa ctgatggccg acagcgcgaa
     2101 catcgtgccg ccggcaaggc cgatcaacgt cgagcgcatg gtgcgagtgc cgccggtgct
     2161 cggcttgagc gagatcacaa taacgccggc ggtcgcgatc agaattgccg ccgccgccaa
     2221 cggcgtcact ctgtcaccga ggaaaataag gccgaacagc gcgacctgca ccggctccgt
     2281 cttgatatag gcgtaggcca ccacgaagga gc
//
LOCUS       DASSRL010000157         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_301834, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000157 DASSRL010000000
VERSION     DASSRL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301834"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..374)
                     /locus_tag="VFL49_03285"
     CDS             complement(75..374)
                     /locus_tag="VFL49_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911027.1"
                     /translation="MNRTMIAVAAGAMTLAGAGSASAQTLVIEGPYAAPVYEVAPAPY
                     YTAPVVVAPAPRVYAAPPAAYGETVIRRGPIAREVVVTEPQTIVEPESGVIYSRW"
     gene            complement(604..1107)
                     /locus_tag="VFL49_03290"
     CDS             complement(604..1107)
                     /locus_tag="VFL49_03290"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7911028.1"
                     /translation="MKVERAADLQRIWDLIKGERTAVLVTMATDGSLDARPMGCVQKE
                     FNGTLWFLTFKDSPKTLEIAEHQQTLVSYARPSDHEFVSVSGRARIVDDPPQVRALWT
                     EGLRVWFPDGPDSPNIALLAIDVEAAKAWTKPASTLTYAYYYLRARMTGEAPSPSQIA
                     KLETFRT"
     gene            1325..1567
                     /locus_tag="VFL49_03295"
     CDS             1325..1567
                     /locus_tag="VFL49_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7911029.1"
                     /translation="MITAGQMRAARALLGIDQRELAERCGLSLPTIQRMEASGGVVRG
                     NVDSLMKLVDALAANGIELIGEGATSSKGGRGVRLK"
     gene            1614..>3533
                     /gene="hyfB"
                     /locus_tag="VFL49_03300"
     CDS             1614..>3533
                     /gene="hyfB"
                     /locus_tag="VFL49_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HET7911030.1"
                     /translation="MVTAALFCVVVLVALSPIAVVLSTSTRAGTFVYAPCLIITLTLV
                     VVGGISLFGQPTSSISLPLGLPWLGAHFRIDALSAFFLIVVNLGGAAASLFALGYGQH
                     EQAPERVLPFYPAYLAGMNLVILADDAFSFLVAWEFMSLTSWALVVAHHRERENLQAG
                     YVYLLMAGFGTLALLLAFGLLAGSNGDYLFESIRVGQPGARVAAIVLVLALIGAGSKA
                     GVVPLHAWLPLAHPAAPSHVSALMSGVMTKVAVYGFVRFAFDLLGPPAWWWSMPVLAI
                     GGATAVMGVLYALMQHDLKRLLAYHTVENIGIIFIGLGLALAFKAHGMADAAALALTA
                     GLLHVFNHSVFKSLLFFGAGAVLNATGERDMEHLGGLIHRMPQTAFAFLVGCVAISAL
                     PPLNGFVSEWLTFQAILLSPQLPSWGLKFLVPAVGALLALSAALAAACFVKAFGVSFL
                     GRPRTPAAANASETDSNSLAAMFFLSALCLVAGVLPGIFIDALAPVTDVLVGVSMPHQ
                     AGLDWLTIVPIAESRSSYNGLLLFVFIAMSGSITAFAIHRLASDKIRRGPAWDCGYPD
                     SSPVTQYTASSFAQPIRRVYGTTVFRAREVGDMPPPGATRAARLSVELRDLVWDAVYA
                     PLAAFIERATERLNVL"
BASE COUNT          601 a         1039 c         1084 g          809 t
ORIGIN      
        1 tcgcgcaaag atggaaaaag aacgttccag gttctgccag gaaccctgga acgttctttg
       61 cgtaagctcg acttttacca gcgcgagtaa ataactccgg actcgggctc aacaatagtt
      121 tgcggctctg tcaccacgac ctcacgtgcg atcggtcctc ggcgaatgac cgtttcgccg
      181 taggcggcgg gcggcgccgc gtacacgcgc ggtgccggcg caaccacgac cggggcggtg
      241 tagtagggcg ccggcgcgac ctcatagacc ggtgcagcat aagggccttc gatgacgagg
      301 gtctgcgccg acgcgctgcc tgcgcccgcc agcgtcatcg cgcctgccgc aacggcgatc
      361 attgtgcggt tcattttgca tctcctcggt ttaagccccg acgggcaatg gcgacgaccg
      421 caacggcgtt cccgccgcat atttggtgtt ttcgagacat ttgaactttg gtctctttgg
      481 cggtgacggg ctcagttggg ccggtatccc ctggcgctac gattctcgat aaggtcggca
      541 gggattgaga agggggaaac aaattatgaa gcggccgatc tgtgagacga cgtggcgccg
      601 ccgtcaggta cgaaaggttt ccagctttgc aatttgagac ggcgaggggg cttctccggt
      661 catccgggcc cgcaagtagt agtaggcata tgtcaatgtg gatgcgggct tcgtccatgc
      721 cttcgcggcc tccacgtcga tcgcgagcaa ggcgatgttc ggagaatccg ggccgtccgg
      781 aaaccagacc cgtaaccctt cggtccagag cgcgcgcacc tgcggcgggt catcgacgat
      841 tcgcgcgcgg ccgctgaccg atacgaattc atgatcggat gggcgggcat aggagacgag
      901 cgtttgctga tgctcggcaa tctcaagagt cttgggtgag tccttgaacg tcaggaacca
      961 cagcgtgcca ttgaactctt tttgcacgca ccccatcgga cgagcgtcga gcgagccatc
     1021 tgtcgccatc gttaccagca cggcggtgcg ctcacctttg atgaggtccc agattctctg
     1081 aaggtcagct gcccgctcca ctttcatgcg ctcctggggc ggggaacgcc ggcaaaggtc
     1141 tctggttcct gcgtggaagg agaccggggt tgccgcgcca cagacgaaaa tcttccgcag
     1201 gagcgcaatg ccaaccttca aattttagat gatgtattaa aaatctaata tgatacttga
     1261 catattataa caactgtatc agttattata aataatgtat tgaatcgcac ggactgtgat
     1321 ccaaatgatt acggctgggc agatgcgggc ggcccgggcg ttgctcggca ttgatcagcg
     1381 cgagctggcg gaacgctgcg gtctttcgct gccaaccatt cagcgcatgg aagcaagcgg
     1441 cggcgtggtt cgcggcaatg tcgattcact gatgaagttg gtggatgcgc tggccgccaa
     1501 tggaatcgaa ctgatcggcg agggcgcaac ctcgagcaaa ggtggccgag gggttcggct
     1561 caaatgagcg cgcgcgccga ggcaacagct tttcgctcaa cgcggaggcg gcaatggtga
     1621 ccgccgcact gttctgcgtg gttgttttgg tggcgctgtc gccgatcgcc gttgtgctct
     1681 caacatccac gcgtgcaggc acgttcgtct atgcgccgtg tctgatcatt acgttgaccc
     1741 tggtcgtcgt cggcggcatc agcctcttcg gccagccgac gtcgagcatc tcattgccgc
     1801 tcggcctgcc atggcttggc gctcatttcc ggatcgatgc gctctccgca ttcttcctga
     1861 ttgtggtcaa tctgggcggc gcggccgcga gcttgttcgc actcggctac ggccaacacg
     1921 agcaagcacc tgagcgggtg ctccctttct atcctgctta tctcgccgga atgaatttgg
     1981 tcatccttgc cgatgacgcc tttagcttcc tggtggcatg ggagttcatg tcgctcacat
     2041 cgtgggctct ggtggtcgca caccaccgcg agcgcgaaaa tttgcaggcg ggatacgtct
     2101 acctgttgat ggcaggcttt ggcactctgg cgctgctctt ggcgttcggt ctgcttgccg
     2161 gcagcaatgg cgactacctc ttcgaatcaa ttcgcgtcgg gcagccaggc gccagggtgg
     2221 ccgcgatcgt ccttgtcctg gcactcatcg gcgccggatc caaggcgggt gtggtcccgc
     2281 ttcatgcatg gttgccgctc gctcatccgg ccgctccgag ccatgtctcc gccttgatga
     2341 gcggcgtcat gaccaaagtg gcggtgtacg gtttcgtgcg ctttgcattc gatctgctcg
     2401 gcccaccggc ctggtggtgg agcatgccgg tacttgccat aggcggtgcc actgcggtta
     2461 tgggagtgct ctacgccctg atgcagcacg atctcaaacg tctgctcgcc tatcacacgg
     2521 ttgaaaatat cggaatcatt ttcatcggcc ttggccttgc gcttgcgttc aaggcgcacg
     2581 ggatggctga tgcggcggcg ttggcgctga ccgccggact gctgcacgta ttcaatcact
     2641 cggtcttcaa gagtcttctg ttcttcggcg ccggtgccgt gctcaatgca accggcgaac
     2701 gcgacatgga gcatcttggc ggcttgattc accgtatgcc gcagaccgcg tttgcgtttc
     2761 ttgtcggctg cgttgcaatc tcggcgcttc caccgctcaa cggtttcgta tcggaatggc
     2821 tgacgttcca ggccatcctg ctcagtccac aattgccttc atggggactc aaatttttgg
     2881 tgccggcggt tggagctttg ctggcgttgt cggcggcgtt ggccgccgcc tgctttgtga
     2941 aagcgttcgg cgtcagtttc cttggccgcc cgcgcactcc ggctgcggcc aatgcaagcg
     3001 aaacggacag caactcgctc gccgcgatgt ttttcctgtc ggcgctgtgc cttgtcgccg
     3061 gtgtgttgcc tggcattttc attgacgcgc tcgcaccggt gaccgatgtg ctcgtcggtg
     3121 tgagcatgcc gcatcaggcc ggactcgatt ggttgaccat cgtgccgatc gcggaaagcc
     3181 gcagttccta caatgggcta ttgctcttcg ttttcatcgc gatgtccggc tcgatcaccg
     3241 cctttgccat tcatcggctc gcctctgata aaataagacg cggaccggct tgggattgtg
     3301 gctatcccga ctccagtcct gtgacacagt acacggcctc gagctttgcg cagccgatcc
     3361 gccgggtata cggcacgacg gtatttcgcg cgcgtgaggt cggcgatatg ccgcctcccg
     3421 gagcgacccg ggcggcgcgt ctcagtgtcg agttgcgtga tctggtctgg gacgctgtct
     3481 acgctccgct cgccgccttc atcgagcgtg ctaccgagcg gctcaacgtc ctg
//
LOCUS       DASSRL010000158         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_704152, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000158 DASSRL010000000
VERSION     DASSRL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704152"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..963)
                     /locus_tag="VFL49_03305"
     CDS             complement(517..963)
                     /locus_tag="VFL49_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7911031.1"
                     /translation="MVGRHGSERSVSGSPRILIIEDEWMIVDLIGGFLREFGYTVRGT
                     AHNINSARREIAKRNYDAVLLDIGLGDEHSPELADLLKEAGTPFAFVTGYDHAFATRH
                     TDVPLLRKPFSPAQLIGLLEQLVRPLANRDAVAGPAPTCGVSSFGM"
     gene            complement(1114..2439)
                     /locus_tag="VFL49_03310"
     CDS             complement(1114..2439)
                     /locus_tag="VFL49_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7911032.1"
                     /translation="MAMAATYGGLSSSEHSAHLRKAVIASTIGTTIEWYDFFIYGTAA
                     GLIFGKLYFPNEDPLTATLAAFGTYFIGFIGRPIGAAIFGHYGDRIGRKATLIATLLC
                     MGIATFLIAFVPTYASIGIWGAVLLTILRMIQGIGVGGEWGGSVLLAMEWSRTHGQRG
                     LVASWPQFGVPCGLFLANLSILAFSTLSGDQFATWGWRIPFALSIILVGIGLWIRLGI
                     LETPVFQQLLDTNKIEQAPIVEVIKRQPREIILSALLRMAEQAPFYIFTAFVFAYAVG
                     TLHMSRDFILTSVLVASVVSFVTIPLSGHISDRIGRRNMYLIGAAVTGIFGFIYFGML
                     GTAIPWVVFLAIVLSLIPHDIQYGPQAALIAEAFTPRLRYSGSSLGYQLASIIAGGPA
                     PLIATALFAAYHSGYAIAVYIAACAVVSLIATALMPDYTGKDISKEYDA"
     gene            2547..2918
                     /locus_tag="VFL49_03315"
     CDS             2547..2918
                     /locus_tag="VFL49_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911033.1"
                     /translation="MTVSRRLEFDKYRRATSTNGPAEALLIRIPGNVLFLWSRQMIDA
                     GRFHWGRLPQSGHQAAGMSWGNVLYRGAAALAVLIVVLAAGNYFYNLSQNRPLIPLIP
                     LVFAGIIWLIGYGLRYLLNDR"
     gene            complement(2992..3282)
                     /locus_tag="VFL49_03320"
     CDS             complement(2992..3282)
                     /locus_tag="VFL49_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET7911034.1"
                     /translation="MTPSRRPPRILVLSWIGLLALLTTTVFAAYLPLGTANTVVALAI
                     ATAKAVIVATVFMELRERNSLAITFAGAGFFWLGILLWLALADYVTRPNWVS"
BASE COUNT          748 a          977 c         1016 g          623 t
ORIGIN      
        1 cgggcaggtc cgaaatattt ctgatgccga cagcgcgttt agcgggcatt ttagacttgc
       61 gacgatttag acttgcgacg atccggaagt ccgttatccg cgatccttca aggatctcgg
      121 atgagcccct ctttcatgaa cgggtgaacc gcccgcctgg ttccaattcg cgtggagagc
      181 cgaacgaacc cacgcatcga cgccaatgcc gccgtgagcg aatgcaattg tccaaggtga
      241 cacgaacggg gaaatggccg gcagaacctg ataaatttcc aggctagagc aagatcgttt
      301 caaactgaat cgtcatgccc gcgcatagcc gtccgaagga cggcgtgctt tcgcacgcct
      361 atgtcgcggg catccacgtc ttaaagttcg tgcaaaatca aaaacgtgga tggtcggaac
      421 aagtccggcc atgacggcgt atagcttcaa tctcagcgga ctctgatcta gcgccgtgcc
      481 cggaacgtcg catgatcgaa gggtctcgat cgcggcttac attccaaagc tggaaacgcc
      541 gcaggtgggg gccggtcctg caaccgcatc gcggtttgca agaggccgaa caagttgctc
      601 caacagtcca atcagctgcg ccggcgaaaa cggcttgcgc agcagcggaa catcggtgtg
      661 tcgcgttgca aaggcatggt cgtagccggt tacaaacgcg aaaggcgttc ccgcttcctt
      721 aagcaggtcg gcgagctcgg gactgtgctc gtcgccaagt ccgatatcga gaagcaccgc
      781 gtcgtagtta cgctttgcga tctcgcgacg agcggaattg atgttgtgcg ccgttccccg
      841 gaccgtgtac ccgaattcgc gaaggaaacc gccaatcaga tcgacgatca tccattcatc
      901 ctcaataatg aggatacgag gggagccgga taccgaacgc tctgagccgt ggcgaccgac
      961 catgcgacac tccaggtcgc ggcaggaata gagtggctct cttgtgcatc gacttgccgg
     1021 aaccacgctc ctgtcgacga cggacactat gtcgacttgc cgatcggcaa gtcacgttac
     1081 aaagtgttca tggtcacgtg ttgtttcggc gcgctatgcg tcatactcct tcgatatgtc
     1141 cttgcccgtg taatcgggca tcagcgccgt cgctatcagg ctgaccaccg cgcacgccgc
     1201 gatgtagaca gcgatcgcgt agccggagtg ataggcggcg aacagagccg tcgcgatcag
     1261 cggcgccggc ccgccggcaa tgatcgaggc aagctgatag cccagcgagg agccgctgta
     1321 gcgcaatcgt ggcgtgaagg cctcggcaat cagtgccgcc tgcggtccgt actggatgtc
     1381 atgcggaatg agcgagagca cgattgcaag aaagacgacc cacggaatgg cggtgcctaa
     1441 catgccgaaa tagataaagc cgaagatccc ggtcaccgcc gccccgatca ggtacatgtt
     1501 cctgcgcccg atccggtcgg agatatgacc cgaaagcggg atcgtgacga aggacaccac
     1561 cgaagcaaca agaaccgagg tcagaatgaa gtcccgcgac atgtgcagcg tgccgacggc
     1621 ataggcgaag acgaatgcgg tgaaaatgta gaacggcgcc tgctcggcca tgcgcaacaa
     1681 ggccgacagg atgatttccc tcggttgccg cttgatgacc tcgacaatgg gggcctgctc
     1741 gattttgttg gtatccagca gttgctgaaa taccggggtc tcgagaattc cgagacggat
     1801 ccacaagccg atcccgacga gaataattga aagggcgaag gggatacgcc agccccaggt
     1861 ggcgaactgg tcaccggaga gggtgctgaa cgccaggatc gaaaggtttg ccaggaacag
     1921 gccgcagggc acgccgaatt gcggccaaga cgcaacgagg ccgcgctggc catgcgtgcg
     1981 cgaccattcc atcgccagca ggaccgaccc gccccattcg ccgccgacgc cgatgccctg
     2041 aatcatccgc aggatcgtga ggaggacggc gccccagatg ccgatcgatg cgtaggtcgg
     2101 tacgaatgca atcaggaatg tcgcgatgcc catgcacagc agcgtcgcaa tgagcgtcgc
     2161 cttgcggccg atacggtcgc catagtgacc gaatatggcc gcgccgatcg ggcggccgat
     2221 aaatccgatg aagtaggtgc cgaaggctgc gagcgtcgcg gtcaaagggt cttcgttggg
     2281 gaaatagagc ttgccgaaaa tcagtcccgc agcagtgccg tagatgaaaa agtcatacca
     2341 ctcgattgtc gtgccgattg tggaagcgat gaccgctttg cgcaggtgcg cgctgtgctc
     2401 ggaactggac agccccccat atgtggctgc catggccatg ggacttctcc gtgtcgtttc
     2461 ctctcgcccc acgggtcagg tctcgttcgg acctgtgcct atacgaaata ggttactcga
     2521 cctcggaatg gttccatcac gatagagtga cggtctcgcg ccgccttgaa tttgataaat
     2581 accgccgcgc aacctcaacc aacgggccgg ctgaagcgtt actcatacgc attccgggga
     2641 atgtgctgtt tctttggagc cgacagatga tcgatgcggg ccgttttcat tgggggcggt
     2701 tgccgcagtc cggccaccag gccgccggca tgtcgtgggg aaatgtttta tatcgcggag
     2761 ctgcagctct cgccgtgctg attgtggtcc tggccgcagg caactacttt tacaatctca
     2821 gtcagaacag gccgctcatt cctctcatcc ccctggtgtt cgccgggatt atctggttga
     2881 tcggctacgg cctgcgctat ctcctgaatg accgctgacc ttcgccgaac aattaggccg
     2941 agctggcatt gccgagcacc atggcctttg cgcaggacca ggcgtgagag atcacgatac
     3001 ccaattcggc cgcgtgacgt agtccgcaag tgcgagccag agcaggattc ccagccagaa
     3061 aaatccggcg ccggcgaacg tgatcgccag ggaattgcgc tcgcgcaact ccatgaacac
     3121 cgtcgccacg attacggcct tggcggtcgc gatcgccagg gcaacgaccg tgttcgctgt
     3181 tccgagcggc aggtaggcgg caaaaacggt cgtcgtcagc agcgccagca agccgatcca
     3241 cgacagcacc agaatgcgcg gaggacggcg gcttggtgtc atccgcttct ccccggcagg
     3301 taaatgagag cgaacagaaa gatccagacc agatcgacga agtgccagta aaggcccgcg
     3361 accg
//
LOCUS       DASSRL010000159         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1176635, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000159 DASSRL010000000
VERSION     DASSRL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176635"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1039)
                     /locus_tag="VFL49_03325"
     CDS             complement(257..1039)
                     /locus_tag="VFL49_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7911035.1"
                     /translation="MTGATKENLAPWLTTLALLVLWQLAVVVFHIENFVLPSPIEAIQ
                     ALWINRYPLMMHGLATLGTTLLGFAIAVVIGLLLGILIGSFRLVYVSLYPVLIGFNSV
                     PKAALVPVLVIWFGVGTVPAVIAAFLLSFFPVVVNVAAGFATIEPELLDVLRVLGATR
                     RDMILKIGIPRSLPYFFASLKIAITLSFVGSVIAEIVAGNNGIGNVMLIASSNFNVPL
                     VFAALLVVGVMGVSMYAIFAALESRFTAWSVRGAGLEYATSG"
     gene            complement(1036..1830)
                     /locus_tag="VFL49_03330"
     CDS             complement(1036..1830)
                     /locus_tag="VFL49_03330"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911036.1"
                     /translation="MSFIEIDDVDLRYGEQAGDLASTLALAKTNLGVAENEFVSIVGP
                     SGCGKSTLLKLISGLVRATRGQVRVGGAEVSGPLKSVGMAFQNSTLLPWRNIRDNVLL
                     PLEIVEPYRSQPKSKDAAKRRRADDLLTSVGLADFGDRMPWQLSGGMQQRAQLCRALI
                     HEPAILLLDEPFGALDAFTREELWGVLQNLWMHRKCTVILVTHELREAVYLSDIVHVV
                     SPRPGRIITSKAVPFARPRTLDMSFEPSFVDLVHACRHHIGGGATT"
     gene            complement(2019..3035)
                     /locus_tag="VFL49_03335"
     CDS             complement(2019..3035)
                     /locus_tag="VFL49_03335"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911037.1"
                     /translation="MKHTARLAMLAVLAACVASSGARAETKVRFSLDWIPGAVHSPFF
                     IAYYKGYYKAEGLDVVIDRGKGSVGLVQQLATGVYDIGYPDISVLTDFDAKNPDKSFP
                     LLMVGYEQAPAAIVVLKSSNITQPKQLEGHVLGATANDATFKLFPTFAQHANIDTDKV
                     KIQYIDSKLRETLLAKKQVDAIIGQIFNTKLELKAKGVDPKDVDSFLYRDYGLDLYGN
                     GLAASPQFLKEHPDAVKGFIRASIKGVRDMVANPEEAVQMTLKYEPLLNADIERDRLK
                     LAMECCMLTPNVLKNGFGDVDKARLERGIAILVKGHGLSRSPKADEIFNSSYLPSAKD
                     RMVK"
     gene            complement(3080..>3716)
                     /locus_tag="VFL49_03340"
     CDS             complement(3080..>3716)
                     /locus_tag="VFL49_03340"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HET7911038.1"
                     /translation="EILAGVHEPEGPFGEFTGYASYRSTQNVFVAKRIRMRGDAMYQS
                     VTSGMSKDHILVSCVTREGEILNALRRNLPNVRNVHVPHTTCGAFVAFISMKKTADGE
                     PQMAIMATLGTELYTKYVIVVDDDVDIFDLNDVIWAVATRVRAEKDIFFIPGAKAAIL
                     DPTSDPQNFTVTKMGIDATRPSGRDFAERLVITEEQRERARSILEAAGIKL"
BASE COUNT          752 a         1103 c         1124 g          737 t
ORIGIN      
        1 tagaccccga acagcaccgt gatgttaagc catgtgtttt gtcgcacgac gatcgactta
       61 gatgcggagc ctcaaggctc gcgtcacgat cgcgtagcgc tgttcgttgt gcggaatgtc
      121 caccaggatg gtacatccaa accgaaccac gaaattgtga cgcttggatt ctttggaaga
      181 gccgccagcg gagaccatgg tcgcgaccgc gcgggaagac tcggtggcca gcgtatggtg
      241 gtcaaaaaca gcgaaatcag ccgctggtcg cgtattcgag tccggcaccg cggacagacc
      301 aggcagtgaa gcggctctcc agcgctgcga agatcgcgta catgctgacc cccatgacgc
      361 cgaccaccag gagggcagcg aacaccagcg gcacgttgaa gttggagctc gcgatcagca
      421 tcacgttgcc gatgccattg ttgccggcga cgatctcggc gatcacgctg ccgacgaacg
      481 atagcgtgat cgcgatcttc agcgacgcga agaaataagg aagcgaacgt ggaatgccga
      541 tcttgaggat catgtcgcgg cgggtggcac cgagcacgcg caacacgtcg agcaattccg
      601 gctcaatggt ggcaaagccg gccgcgacat tgaccaccac cggaaagaac gacagcagaa
      661 aggccgcgat caccgccggc actgtgccga cgccgaacca gatcacgagc accggcacca
      721 gcgccgcttt tggtacgcta ttaaagccga tcagaacagg gtagagcgaa acgtagacga
      781 gtcgaaatga gccaatcagg atgccgagca acagcccgat gacgacggcg atggcgaagc
      841 ccaacagcgt ggtgccgagc gtcgccagcc cgtgcatcat caacgggtag cggttgatcc
      901 acagcgcctg aatcgcctca atcggcgacg gcaggacgaa gttctcgatg tggaacacca
      961 cgaccgcaag ctgccacagc accagcagcg cgagcgtggt gagccagggt gcgaggttct
     1021 ctttggttgc gccggtcacg ttgtggcccc accgccgatg tgatgccggc aggcatgcac
     1081 cagatcgacg aaactcggct cgaagctcat gtcgagtgtg cggggccgcg cgaacggcac
     1141 cgctttcgac gtaatgatgc gaccggggcg cgggctgacg acgtgcacga tgtcagagag
     1201 atacaccgcc tcgcgtaatt cgtgcgtcac taggatgacc gtgcacttgc ggtgcatcca
     1261 gagattctgc agtacgcccc acaattcctc tcgggtgaag gcatcgagtg cgccgaacgg
     1321 ctcgtcgagc agtaggatcg ccggctcatg gatcagcgcg cggcacaact gtgcgcgctg
     1381 ctgcatgccg ccggagagtt gccacggcat acggtcgccg aaatcggcga ggccgacgct
     1441 ggtcagaagg tcgtcggcgc gccggcgctt ggccgcatct ttgcttttcg gttggctgcg
     1501 gtacggctcg acgatctcga gcggcagcag cacgttgtcg cggatgttgc gccacggcag
     1561 cagagtggag ttctgaaacg ccatgccgac gcttttgagt gggccactta cttcagcgcc
     1621 gccgacgcga acctggccgc gtgtcgcccg cacgagcccg gagatcagtt tcagcagggt
     1681 cgacttgccg cagccgctcg ggccgacgat cgaaacgaat tcgttctctg cgacgccaag
     1741 gttggtctta gccagagcca atgtgctggc caggtcgccc gcctgctctc cgtagcgcag
     1801 atcgacgtcg tcgatttcga tgaagctcat gcggctacca acagataagc aggaggtcaa
     1861 gcaagcggtc cgttccccgc cgcgctgcag cgcgtggtgc cgtcgtgcag acgcgggtcc
     1921 cggtccttgt aggggcgggt tgaaacctgt ccctaccctt caagagatct accggatttc
     1981 ggatcagagt cgtacatcgc gttcggggac acgcgcagct acttcaccat acggtccttc
     2041 gccgacggca ggtacgacga attgaagatc tcgtcggcct ttgggctgcg cgacagcccg
     2101 tggcctttga ccagaatggc gatgccacgc tcgagacgtg ccttgtcgac gtcgccgaag
     2161 ccgttcttga gcacgttcgg cgtgagcatg cagcattcca tggcgagctt gagccgatcg
     2221 cgctcaatat cggcattgag cagcggctcg tatttcagcg tcatctgcac cgcctcttcg
     2281 ggattggcga ccatgtcgcg cacccccttg atgctggcgc ggatgaagcc cttcaccgca
     2341 tcgggatgtt ccttgaggaa ctgcggcgat gcggcaaggc cgttgccata gagatcgagt
     2401 ccgtagtcgc ggtagaggaa cgagtcgaca tctttcgggt cgacgccctt ggccttgagc
     2461 tcgagcttgg tgttgaagat ctgcccgatg atagcgtcga cctgcttctt cgccagtaag
     2521 gtctcgcgca acttcgaatc gatgtactgg atcttcacct tgtcggtgtc gatattggca
     2581 tgctgggcga acgtcggaaa taacttgaat gtcgcatcat tggccgtggc gccgagcacg
     2641 tgtccttcga gctgcttcgg ctgcgtgatg ttactcgact tgagcaccac tatcgcggct
     2701 ggcgcctgct catagccgac cataagcagt ggaaagctct tgtccggatt ttttgcgtcg
     2761 aaatcggtga gaacactaat gtcgggatag cctatgtcat agaccccggt cgccaactgc
     2821 tgcaccagcc cgaccgagcc tttgccgcgg tcgatgacga cgtcaaggcc ttcggccttg
     2881 tagtagcctt tgtaataggc aatgaagaac ggcgaatgca ctgcccccgg aatccagtcg
     2941 agcgaaaaac gcaccttcgt ctcggcgcgc gcgccgctcg atgcgacaca tgcggccaac
     3001 acggccagca tggctaaacg tgctgtgtgc ttcatcttct ccctccgcct tgcttggttc
     3061 tttcgatgtc tgtcgcccgc taaagcttga tacccgccgc ctccagaatg gaacgcgccc
     3121 gctcacgctg ctcttccgtg atcaccagcc gctcggcaaa atcgcggcct gacgggcgcg
     3181 tagcgtcgat gcccattttg gtgaccgtga aattctgcgg gtcggatgtc ggatctagga
     3241 tggcagcctt cgcgcccggt ataaagaaga tgtccttctc cgcgcggaca cgtgttgcga
     3301 ctgcccatat cacgtcgttg agatcgaaaa tatcgacgtc gtcgtccacg acaatcacgt
     3361 attttgtgta aagctcggta ccgagtgtcg ccatgatggc catctggggc tcgccatcgg
     3421 ccgtcttttt cattgatatg aatgcaacaa atgcaccaca tgtcgtgtga ggaacgtgca
     3481 cattgcggac gttgggcaga ttgcgccgca aggcgttcag gatttcgcct tcgcgcgtca
     3541 cgcacgatac taagatgtga tctttggaca taccagaggt gacgctttgg tacatcgcat
     3601 cgccacgcat gcgtatgcgc ttggcaacga agacattttg cgtgcttcgg tacgacgcgt
     3661 agccggtaaa ttcgccgaac ggcccttccg gctcgtgcac cccagcgagt atttca
//
LOCUS       DASSRL010000160         4260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1509154, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000160 DASSRL010000000
VERSION     DASSRL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509154"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFL49_03345"
     CDS             complement(<1..182)
                     /locus_tag="VFL49_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HET7911039.1"
                     /translation="MTTLRTVTQLCEHDLVPPERRRAIEKVAARYAVALPEALAGLID
                     KNDRSDPIARQFVPGAA"
     gene            complement(203..1246)
                     /gene="epmA"
                     /locus_tag="VFL49_03350"
     CDS             complement(203..1246)
                     /gene="epmA"
                     /locus_tag="VFL49_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HET7911040.1"
                     /translation="MPGSPPFWSRDTHAARRPYLLARGRIMAALRNWFATRDFVEVET
                     AALQLSPGNETHLHAFSTDLIGPGGEHRRLYLRTSPEFACKKLLAAGETRIVDFARVF
                     RNRERGALHHPEFTMLEWYRAHEPYEALMDDCAGLLAEAARAAGTAQFVYRGKTIDPF
                     AAPERLTVADAFSRHAGIDLLATVRNGKGDRAALAAQATKAAVAIAENDNWSDIFSRI
                     LVERVDRHLGIGRATILYEYPLPLAALARAKPGSDKVAERFELYVCGVELANAFGELR
                     DAAEQRIRFEAAMAEKEGLYHERYPIDEDLLAALARMPPASGVALGLDRLVMLASGAQ
                     RVDQVIWTPVVES"
     gene            1358..1924
                     /gene="efp"
                     /locus_tag="VFL49_03355"
     CDS             1358..1924
                     /gene="efp"
                     /locus_tag="VFL49_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7911041.1"
                     /translation="MKVIASSLRKGNIVDIDGRLYVVLTAENFHPGKGTPTTQVDMRR
                     ISDGVKTSQRYKTTDQVERAHVEDHEFSYLYQDGDGFHFMNQETFDQVQVPADIIGSQ
                     SAYLQEGMVVNLSVHEGVPVAIELPQKVTLEIVDTEPVVKGQTASGSFKPAKLSNGVR
                     TMVPTHITVGTRVIVSTADGSYVERAKD"
     gene            complement(1921..2985)
                     /locus_tag="VFL49_03360"
     CDS             complement(1921..2985)
                     /locus_tag="VFL49_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911042.1"
                     /translation="MTVREANLTPASSADREPELIESGGTARLHLTLATTDYDHVRDV
                     VTGVVRPEGIILTAFVLPVEEVFYRFIKNREWDVSEMSFGKFIGFASQGNSPFVGIPV
                     FPSRVFRHSAFYVRADRRIGTPKDLEGKRVGIPEWAQTAGIYARGFLSETAGVDLRKI
                     QWVQAGMNEAGREEKIEFALPAGIQYEQRRDTSISAMLLSGEVDAAISARVPDAFEKG
                     GGKIVRLYPDYRGEEARYQVATGIFPIMHVIAMRRAVFDRYPWVAMNLFKAFEEAKQR
                     SLGRVRDLTASRIPVPWSAALSGEWSRNFGDDPFPYGLEGNRKTLEAFCRFAHTQGIT
                     AKQLAPDDLFPKEVRASVRV"
     gene            3059..>4260
                     /locus_tag="VFL49_03365"
     CDS             3059..>4260
                     /locus_tag="VFL49_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HET7911043.1"
                     /translation="MPEFDAFNSAADVNPWLTGQSEGSGLKRAILAGLALLIAGAAPA
                     QEMKSPRILVAKVDWDAAAASIADKPAGTPAEAFAKLNAAASISFPGIAESPVPVLLP
                     FDVGAFIKDRSANPEPAVENADRFMQSGFRSTKFFMAGPAGYDTAFALTVADVRDLSD
                     IRYAEPVYVLLSGLAMIYELDGPAIPEGELVKSLQDEYPGIRRYLHESYIRYSFERFG
                     ATYVAAIYCRDTRPRSKILTCKQADRVMDRFLRALKLAGGKPSEAPPVNEPLALDRPA
                     DVSKDFTYFSPGFIIPNTGLKKDTGGRGDYTVYARLRFPIKDAPAFANSQSFNNWGDC
                     DFTGRSVRPRRKDQSYSCKVNGRPLVFNESAPGNYSYPWRDNFCEHRHYFVGQCPGGE
                     GHQGQDIRP"
BASE COUNT          819 a         1380 c         1263 g          798 t
ORIGIN      
        1 gccgcgccgg gcacgaactg acgcgcaatc ggatcgctcc gatcgttttt gtcgatcagg
       61 ccggctaagg cctcgggcaa ggcgaccgca taacgtgccg caactttttc gatcgcccgg
      121 cggcgctcgg gcggcacaag atcgtgctcg caaagctgcg ttaccgttcg taacgttgtc
      181 atagttcacc tcgttctttt tgctaggact cgacaaccgg ggtccagatc acctggtcaa
      241 cgcgctgtgc gccgctcgcc agcatgacca gccggtcgag cccgagcgca actccgcttg
      301 ccggcggcat gcgggccaat gccgcgagca aatcctcatc gatcggatag cgctcgtgat
      361 agagaccttc tttttccgcc atcgccgctt caaaacgtat ccgctgctcc gcagcgtcac
      421 gcagttcacc gaacgcgttg gcgagctcaa ccccgcagac atagagttca aagcgctcgg
      481 cgaccttatc gcttccgggc tttgcccgcg ccagcgccgc cagcggcagg ggatactcat
      541 ataatattgt cgcgcggcca atccccagat gccgatcgac ccgctcgacc aaaatgcggc
      601 tgaaaatgtc gctccaattg tcgttctcgg ctatggcaac cgcggctttt gtggcctgcg
      661 cggcaagcgc ggcgcgatcg cctttgccat tgcggacggt cgccaagagg tcaatgccgg
      721 cgtgacggga gaatgcgtcg gcgaccgtca atcgctctgg ggccgcaaag gggtcgatcg
      781 tcttgccgcg gtagacgaat tgcgccgtac cggcggcgcg agccgcctcc gcgagcaagc
      841 cggcacaatc atccatcaac gcttcgtaag gctcgtgcgc cctgtaccac tcgagcatgg
      901 tgaattccgg gtgatgcagg gctccgcgtt cgcggttacg gaagacgcgg gcaaaatcga
      961 cgatgcgcgt ctcgcccgcc gccaataatt tcttgcaggc gaattccggc gacgtgcgca
     1021 ggtacagccg gcggtgctct ccgcccggtc cgatcagatc ggtcgaaaag gcgtgcaggt
     1081 gcgtctcgtt gccgggagac agctgcaatg ccgccgtctc gacctcgacg aaatcacgtg
     1141 tggcgaacca attgcgcagg gcggccatga tgcgtcctcg cgccaacagg tagggtctgc
     1201 gggccgcatg agtgtctcgg ctccagaacg gcgggcttcc cggcacattt acctctgtca
     1261 aacggcacac gggaaagact ggaatcggcc gcgaagatcg ctatggtgcg gcccgaaacc
     1321 gcggcccact gccgcacata ggttaaggat tacaaccgtg aaggtcatcg cttcttcgct
     1381 ccgtaagggc aatatcgtgg atatcgacgg caggctctat gtcgttttga ccgcagaaaa
     1441 ttttcatccc ggcaagggca ctccgacgac ccaggtcgat atgcgccgga tttcggacgg
     1501 cgtgaagacg tcgcagcgct acaagaccac cgatcaggtc gagcgcgccc atgtcgagga
     1561 ccatgaattc agctatctct atcaggacgg cgacggattt cacttcatga atcaggagac
     1621 cttcgaccag gtgcaggtgc ccgccgatat catcggcagc cagtcggcct acctgcagga
     1681 aggcatggtg gtgaacctca gcgtgcacga aggcgtgccg gtcgcgatcg agctgccgca
     1741 aaaggtgacc ctcgagatcg tcgacaccga accggtcgtg aagggccaaa ccgcttccgg
     1801 ctcgttcaag ccggccaagc tgtcgaacgg cgtacgcacg atggtcccga cccatatcac
     1861 ggtcggcacc cgtgtcatcg tgagcaccgc cgacggatca tacgtcgagc gtgcgaagga
     1921 ctagacgcgg acgctcgcgc gcacctcttt cggaaagaga tcatcgggcg caagctgttt
     1981 tgccgtgatg ccctgcgtgt gggcgaaacg gcagaatgct tcgagcgtct tgcggtttcc
     2041 ctcgagcccg tagggaaacg ggtcgtcgcc gaaattcctg ctccactcgc cgctgagcgc
     2101 ggcagaccag ggcaccggga tgcgcgacgc cgtcagatcc cgcacgcgtc caagacttcg
     2161 ctgcttggcc tcctcgaaag ccttgaacag gttcatcgcc acccacggat agcggtcgaa
     2221 cacggcgcgg cgcatcgcga tcacgtgcat gatcggaaag attccggtcg ctacctggta
     2281 gcgcgcttcc tcgccgcgat agtccggata gagccgcacg atctttccgc cgcctttttc
     2341 aaatgcatcg ggcacgcgcg ccgaaatcgc tgcgtcgacc tcgcccgaaa gaagcatggc
     2401 ggaaatgctg gtgtcgcgcc gctgctcata ttgaatcccg gccggaagcg cgaactcgat
     2461 cttctcttcc cgtcccgcct cgttcatgcc ggcctgcacc cactgaatct tgcgcaagtc
     2521 gacgcccgcc gtttccgaga ggaacccgcg cgcatagatg ccggcggtct gtgcccactc
     2581 cggaatgccg acgcgcttgc cctcgagatc cttcggcgtg ccgatgcggc ggtccgcgcg
     2641 cacgtagaac gccgagtgcc ggaagacgcg cgacgggaaa accggaattc cgacgaaggg
     2701 cgagttgccc tgggaggcaa agccgatgaa cttgccgaag gacatttccg agacgtccca
     2761 ctcccggttt ttaataaacc tgtagaaaac ttcttcgacc ggaagcacga aggcggtcag
     2821 gataatgcct tcagggcgaa cgaccccggt caccacgtcg cgcacatggt cgtaatcggt
     2881 ggttgcaagc gtcagatgca ggcgcgcggt accgccactt tcgatcaact ccggctcacg
     2941 gtccgcgctt gaggccggag tcagattggc ttcacgcacc gtcatttcca ttcatctccc
     3001 gtcgcaggca tgtcggctgg gcattgcggc gcaccctaca cccgaatgga gcccttgtat
     3061 gccggagttc gatgccttta actccgctgc cgatgtcaac ccttggctga cggggcagag
     3121 cgagggaagc ggattgaagc gtgccattct cgctggcctc gcactgctga ttgccggtgc
     3181 cgcgccagcc caggagatga agagcccgcg catcttggtc gcaaaggtcg actgggatgc
     3241 cgcggccgcg agcatcgccg acaaaccggc gggtacgccc gccgaggctt ttgccaagct
     3301 caacgcggca gccagcatca gttttccggg gattgcggaa agcccggtgc ccgtcctcct
     3361 gccttttgat gtcggcgcct tcatcaagga ccgcagcgcc aatccggagc cggccgtcga
     3421 gaacgccgat cgcttcatgc aatcgggatt tcgatcgaca aaatttttta tggccggccc
     3481 cgccggctat gacacggctt tcgcgctgac cgttgccgat gtccgggacc tttccgacat
     3541 ccgctacgcc gagccggtct acgtcctctt gtcgggcctg gccatgatct atgagctcga
     3601 cggcccggcg atacctgagg gcgagctcgt gaaatcactt caagacgagt accccggaat
     3661 ccgccgctac ctgcacgaat cctatatccg ctacagcttc gagcgatttg gggcgaccta
     3721 cgtcgcggcg atctattgcc gagacacgcg gccgcgctcg aaaattctaa cgtgcaaaca
     3781 ggctgatcgc gttatggatc gattcctgcg tgcgctgaag cttgccggcg gcaaaccttc
     3841 ggaggcgccg ccggtcaatg aaccgcttgc tcttgaccgc cccgccgacg tctcgaaaga
     3901 cttcacgtat ttcagccccg gtttcatcat tcccaacacc ggcctgaaaa aggacaccgg
     3961 cggccgcggc gattacaccg tctacgcgcg cctgcgcttt ccgatcaagg atgccccggc
     4021 cttcgcgaac tcgcagtcgt tcaacaattg gggcgattgc gatttcaccg gccgctcggt
     4081 gcgcccgcgc cgcaaggacc agtcctactc atgcaaggtg aacggcaggc cgctcgtctt
     4141 caacgaatcc gcgcccggca actactctta tccgtggcgc gacaactttt gtgaacatcg
     4201 gcattatttc gtcggccaat gccccggcgg tgaaggtcac cagggccagg acatccggcc
//
LOCUS       DASSRL010000161         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_406946, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000161 DASSRL010000000
VERSION     DASSRL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406946"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1058
                     /locus_tag="VFL49_03370"
     CDS             141..1058
                     /locus_tag="VFL49_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911044.1"
                     /translation="MTLFIEQCLNGLQFGLLLFMLAAGLTLVFGIMDLVNLAHGSLYM
                     IGAYFAATFSAWTGSFLLGVAIALAGTLVVGMILEVVVMRRLYGRDHLDHVLATFGLI
                     LFFDESVRLIWGPAGLSLPLPSWLNAPVQILPGIYYSAYKLSIITVSLAVAAGLYFLV
                     MRTRVGMLIRAGASNREMVGALGVNIKLLYTLVFGLGAALAGLAGLMQAPILTVQIGM
                     GEIIIILAFVITVIGGIGSIRGAFVAAIFVGLVDTLGRAFFPDLLRLVLSPAAASTAA
                     PALSSMLIYLLMAIVLVLKPEGLFSAGNK"
     gene            1055..2002
                     /locus_tag="VFL49_03375"
     CDS             1055..2002
                     /locus_tag="VFL49_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911045.1"
                     /translation="MSGWLSTRTLTVIVLLAALALLPVYTEITGSRFLLTLFTRIVIL
                     ALAAVSLNLILGYGGMMSFGHAVYLGIGGYSVGILAHEGIYSGFVQWPVALVASALFA
                     LVIGALSLRTRGVYFIMITLAFAQMAYYVVAGLARYGGDDGLTIYKRSQFFEPINLSN
                     KTQFYYICLVLLYASIYLVWRIVNSRFGMVIQGSRSNDTRMRAIGFPTFRYKLTCFVI
                     AGTLCGLAGALLANHTDFVSPAMMYWTRSGDLIIMVVLGGMGSVIGPVFGAIALLVLE
                     ETLSGITEYWQIILGPLLLLVVLFARGGIDGLLGKVRYA"
     gene            1995..2753
                     /locus_tag="VFL49_03380"
     CDS             1995..2753
                     /locus_tag="VFL49_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911046.1"
                     /translation="MPDLLQVENLAKRFGGIVATDDLSIAVPEGQLHAVIGPNGAGKT
                     TLIAQLSGQLSPDSGRIRFAGTDITRSPTPMRSALGLARSFQITSLFLDLSVLDNVAL
                     AVQAHTGHSFHFWRDARREPELRDPARAAIARVGLKVRENLPASALSHGEHRQLELAM
                     ALASKPRMLLLDEPMAGLGPEESARMVVMLRELKKELTILLVEHDMEAVFALADRITV
                     LVYGRVIASGKPEEIRNNAQVRDAYLGEQEAVHG"
     gene            2746..3453
                     /locus_tag="VFL49_03385"
     CDS             2746..3453
                     /locus_tag="VFL49_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911047.1"
                     /translation="MAEPVLALEGIETCYGLSQVLFGVSLVIAPGEMVTLMGRNGMGK
                     TTTVRSIMGLTPARAGAIRFMGSDIRGLPSYKVAQCGIGLVPEGRQIFPNLSVRENLL
                     ATAVRRDGAEWTLEKVYALFPRLAEREGSMGNQLSGGEQQMLAIGRALMTNPRLLILD
                     EATEGLAPLIRAEIWRCLASLKKLGLSVLVIDKNVGARLRVADRHFLIERGRVVWKGT
                     SPELATTPDVQHRYLGV"
BASE COUNT          597 a         1086 c         1143 g          800 t
ORIGIN      
        1 tacgatcgtc aaggacttcc aggacattca tcactcgaag tgcaagatgc cggcaaactg
       61 atgccggcgc ggtcaatgac ggcggcggcg cctgcgcgct gccgccgttt tcaatagcga
      121 atgcgtgaag tctgcgcgct atgacgctgt tcatcgagca atgcctcaac ggcctgcaat
      181 tcggcctgct gctgttcatg ctggcggccg gcttgacgct ggtgttcggc atcatggatc
      241 tcgtcaacct ggcgcacggc tcgctctaca tgattggcgc ctattttgcc gccaccttct
      301 ccgcatggac cggcagtttc ctgctcggtg tcgccattgc gctggccgga acgcttgtgg
      361 tgggcatgat tctggaagtc gtcgtgatgc ggcgacttta cggccgtgat cacctcgatc
      421 atgtgctggc aacgttcggc ctgatccttt ttttcgatga gtctgtgcgg ctgatctggg
      481 gaccggcggg cttgagcctg ccgctgccgt cgtggctcaa cgcgccggtg caaattcttc
      541 ccggcatcta ttactcggcc tacaagctct cgatcataac cgtgtcactt gcggttgcgg
      601 caggccttta ttttctggtc atgcgaacgc gcgtcggcat gctgattcgt gccggcgcct
      661 cgaaccgcga aatggtgggc gcgctcgggg tcaacatcaa gctgctctac acgctggtat
      721 tcgggctcgg cgcggcgctc gccgggcttg cagggttgat gcaggccccg atcctcaccg
      781 ttcagatcgg aatgggtgag atcatcatta ttctcgcctt cgttattacg gtcatcggcg
      841 gaatcggctc gatccgcggc gcgttcgtgg ccgcaatttt tgtcggattg gtcgacactc
      901 ttggccgggc cttttttccc gacctgctgc gcctcgtgct gagtccggca gctgcgtcga
      961 ccgctgcgcc ggcgctgtcg tcaatgctga tctacctgct gatggcgatt gtgcttgtgc
     1021 ttaagcccga aggcctattc tcggcgggca acaaatgagc ggctggctgt ctacgcgcac
     1081 gctcacggtc atcgtgctgc tcgccgcact ggcgctcttg ccggtgtata ccgagatcac
     1141 cggaagccgc ttcctgctca cgctgttcac gcgaatcgtc attctggcgc tggcggcggt
     1201 cagcctcaat ctgatcctcg gatacggcgg catgatgagc ttcggtcatg ccgtctatct
     1261 cggcatcggc ggttactcgg tgggcattct cgcacatgag ggcatctaca gtgggttcgt
     1321 gcaatggccc gtcgcacttg tggcatccgc actttttgcg ctggttatcg gtgcgttgag
     1381 cctgcgaacg cgcggcgttt acttcatcat gatcactttg gcatttgcac aaatggccta
     1441 ttacgtcgtg gcgggcctcg cgcgctacgg cggcgacgat gggcttacga tctacaagcg
     1501 cagccaattc ttcgagccga tcaatctgtc gaacaagacg cagttttatt acatctgcct
     1561 ggtgctgctt tacgcctcga tctatctggt gtggcggatc gtgaattcgc gcttcgggat
     1621 ggtcatccag ggctcgcgct cgaacgacac acgcatgcgc gcgatcggat ttccaacgtt
     1681 tcgatacaag ctgacctgct tcgtcattgc cggcacgctg tgcgggcttg ccggcgcgtt
     1741 gctcgccaac cacaccgact tcgtcagccc tgcaatgatg tattggacgc gctccggtga
     1801 cctcatcatc atggttgtgc tcggtggcat gggttcagtg atcggtccgg tattcggcgc
     1861 tatagcgctt ctggtgctcg aggagacgct gtcgggcatt acggagtatt ggcagatcat
     1921 cctggggcca ttgctgctgc tggtggtgct gtttgcgcgc ggcggcatcg acggtctgct
     1981 cggcaaggtg cgctatgcct gacctgctgc aggtggaaaa tcttgccaag cgcttcggcg
     2041 gcatcgttgc gaccgacgac ctcagcatcg ctgttccgga agggcagctt catgcggtga
     2101 tcggaccgaa cggggccggc aagaccacgc tcatcgcaca actcagcggg cagcttagcc
     2161 ccgattccgg ccgcatccgg tttgccggca cggatatcac caggtcgccg acgccgatgc
     2221 gcagtgcgct aggccttgcg cgctcgtttc agattacgtc gctgtttctc gatctctcgg
     2281 ttctcgacaa cgtcgcgctg gccgttcagg cgcataccgg gcattcattt cacttctggc
     2341 gcgatgcgcg ccgcgagccc gaactgcgcg atccggcccg cgcggcgatc gcccgcgtcg
     2401 ggctcaaagt acgggaaaat ctgccggcgt ctgcgctcag ccacggcgaa caccgtcagc
     2461 tcgaactggc gatggcactg gcttcgaagc cgcgcatgct tttgctggat gagccaatgg
     2521 cgggactcgg tccggaggaa tcggcgcgca tggtcgtgat gctgcgcgag ctcaaaaagg
     2581 agctgacgat cctgctggtc gagcacgaca tggaggctgt attcgccctt gccgatcgca
     2641 tcaccgttct tgtctatgga cgtgtcatcg cttcggggaa gccggaagaa atccgcaaca
     2701 atgcgcaggt acgcgacgcc tatctcggcg agcaggaggc cgtccatggc tgaacctgtg
     2761 ctggcgcttg agggaatcga aacctgctat gggctgagcc aggtgctgtt cggggtttcg
     2821 ctggtcatag cgccgggcga gatggtgacc ctgatgggcc gtaacggcat gggcaagacg
     2881 accaccgtgc gttcgatcat ggggctcacg ccggcgcgag ccggtgcgat acgcttcatg
     2941 ggcagcgata tccggggtct gccgtcatac aaggtggccc aatgcgggat cggcctggtt
     3001 ccggaaggcc ggcagatctt tccaaacctt tccgtgcgcg aaaatctgct cgcgacggcg
     3061 gttcgccgcg acggcgccga atggacgctc gaaaaggtct acgctttatt cccgcggctt
     3121 gccgaacgtg agggcagcat gggcaatcag ttgtccggcg gcgagcagca gatgcttgcg
     3181 atcggccgcg cactcatgac caatccgcgg ctgctgatac ttgacgaggc gacggagggt
     3241 ctggcgccac ttatccgcgc ggagatctgg cgttgccttg ccagcctcaa aaaactcggc
     3301 ctgtcggtcc tggtgatcga caaaaatgtt ggtgcacgtc tgcgcgtcgc cgaccgtcac
     3361 ttcctgatcg agcgggggcg cgtggtgtgg aaagggacct caccggagct tgccacgacc
     3421 ccggacgtcc agcaccggta tttgggcgta tagcctaccc cgccggcaat ggcggaccgc
     3481 ggcggcggac caaggtgaag cttgccccgc cgagcctgcg atgcagccgg tcaagcagca
     3541 gatagatcac cggcgtcgtg tagagcgtaa gcatctgcga caccaggagg ccgccgatga
     3601 tggtgatgcc tagcggtcgt cgcagc
//
LOCUS       DASSRL010000162         6355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1019587, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000162 DASSRL010000000
VERSION     DASSRL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019587"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..378
                     /locus_tag="VFL49_03390"
     CDS             181..378
                     /locus_tag="VFL49_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911048.1"
                     /translation="MGRAILAISGSAISLLPRPFTAIGSIFAGFWRELTRDAVRPYRP
                     ELHYMRGPGPAWRAKHGLPSV"
     gene            454..1413
                     /locus_tag="VFL49_03395"
     CDS             454..1413
                     /locus_tag="VFL49_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HET7911049.1"
                     /translation="MVRAAILDDYQNVAMGFADWSPVGKDVEIKVFNKPFGSQDEAIK
                     ALQGFAVVVGMRERTPFPRKVIEALPDLKLLITTGARNNSFDIKACAERGITVCGTGG
                     VGSPTTGVAFGLMLELTRRIGFENARLKAGAPWQVTIGRDLEGLTLGILGLGKLGQRS
                     ATVGKAFGMKTIAWSQNLTEEKAKAAGAGYVSKDDLFRNADIVTIHLVLSDRSRGLVG
                     AKELGLMKKSAYLINTSRGPIVDEKALIAALQSKSIAGAGLDVFDIEPLPLDHPFRKM
                     DNVVITPHLGYVSEQNYRKFFPDIVEDIRAWLDGKPVRVIEAK"
     gene            complement(1418..2368)
                     /locus_tag="VFL49_03400"
     CDS             complement(1418..2368)
                     /locus_tag="VFL49_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7911050.1"
                     /translation="MVKKIALEEHFLDPATEPYWRPTMVDVAPAKTAQLYAALTDFGE
                     QRLMMMEQHGIARMILAVAGPGAQAERDTATAVRAARASNDFLAKEIQKRPNRYSGFA
                     HLAMQDAKAAADELERCVRDLKFCGVMINGHTNGKYLDDRSYDAFWERAAALDAPVYI
                     HPADPVAPLPVLAGHNVLVRPMWGWGVETGSHALRVISGGVFHRYPKAKLLLGHLGET
                     LPFLLWRFDSRSKPYGWNLPKPPSQYIKENVWVTTSGMYDGPPLHCSIGALGRDHVMF
                     SADYPFDAEGAGPFMDAVPLDESIRADVAYNNAARLFRLA"
     gene            2497..3267
                     /locus_tag="VFL49_03405"
     CDS             2497..3267
                     /locus_tag="VFL49_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7911051.1"
                     /translation="MNRLVTNDKSQVQVIARAATILRALEDENSGLSLGQIAQRVNLA
                     RSTVQRIVAALETEKLVIAATPNGRVRLGPTILRLAASVRSDFVAMARPFLERLSEEL
                     HETVDLSTVKKDHLIFIDQVIGAQRLRAVSAVGDTFPLYCTANGKAYLAQLTDKAIET
                     LIGRTYEARTPKTLTRFDALLADLKSARRNGVAYDREEHTLGICAVGVAMHDPLGNAI
                     AISVPVPSQRFASRQTLVAERLVATKNALEAHMNSSAA"
     gene            3313..4155
                     /locus_tag="VFL49_03410"
     CDS             3313..4155
                     /locus_tag="VFL49_03410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7911052.1"
                     /translation="MAKKIRGVMFEENLKNSMGLEFYNLSHRFGYQSPNWPYFEDVKI
                     ERIHYMAKSGVLSQRITTSMHNTTHIDAPAHVVQGTPFIDEVPLPHFFGSGIVVSIPK
                     NKWEPITGEDLEKACGKIVRPRDVVIVNTGWHKFYEDSEDYFCRAPGFVPSAGEWFVK
                     KKVKVVGHDTQANDHPLATAIGPQRNGPLHPHLADEYREWSGGRDWKDDFPEWEPVHR
                     ILFKNGIMGIENVGGDIDKVTGKRCTFAYFPWNWERGDGCIVRLVAIADPTGKFRIER
                     GRKF"
     gene            4155..4493
                     /locus_tag="VFL49_03415"
     CDS             4155..4493
                     /locus_tag="VFL49_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7911053.1"
                     /translation="MIIRNLSDAKPYEAPNHRGYESLRIFGAEMGGSQALVFGISHFR
                     PGGGAGPDGSPPEKIYHVLKGELTVIAGGKEQVAKPGDTVFIAPNENREIVNRGKDVC
                     TILVAVAAPK"
     gene            4550..5365
                     /locus_tag="VFL49_03420"
     CDS             4550..5365
                     /locus_tag="VFL49_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911054.1"
                     /translation="MSNDFLKNPQSLFDIKGKTAIVTGASGAFGALAAKTFAGAGANV
                     VLAASKADELKKVAAECEKLGGKTEVIALRPSSEENCEKIVKAAVDKFGRVDILVVAS
                     GLNKVSKIVDQKVEDFLDVMDANVNQSWLMARAAGKQMLKQGQEGKPGGKVILTSSAR
                     GLLGHPAGYSAYCASKSAVDGITKALGCEWGETGITVNALGPTVFRSPLTAWMYEDTE
                     RAQTVRKGFLARVPKGRLGEPEDLAGPLLFLASKASDFYTGHILYADGGYTAG"
     gene            5368..6288
                     /locus_tag="VFL49_03425"
     CDS             5368..6288
                     /locus_tag="VFL49_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET7911055.1"
                     /translation="MAKARIAVIGAGLMGHGIAQVFALAGHEVMITDAVTANLDSAKT
                     RILTNLRDLGDDQKAVEHVAPVPELSQAVRNADYVVEAVLEDLPLKQKLFVEIEKHVR
                     PNTILSSNTSVIPITKIMEGLAHRERALGTHWWNPPYLVPLVEVIETQWTSPQAVDFT
                     MALHSVAGKKPAHVKKDVPGFIGNRLQHALWREAVALVEQGICDAETVDDVIKSAFGR
                     RLAVLGPLENADMVGTDLTLAIHKTVLPDIDSRPSPSPYLEKLVKDGKLGFKSGEGFR
                     KWSPEQQAALRSKVLNHLRNARSEDTKSAR"
BASE COUNT         1318 a         1905 c         1954 g         1178 t
ORIGIN      
        1 gtgcgatcga agtaaatctc tggaaaggct gattgcagat agggcaccgg tatgacgttc
       61 aggtacctgt tcttaaccgc ttcggtttca tgatgcccac gcatgaacct cggcgccgga
      121 cccggcgacc aagggacaag aggacgggat tgtccgcctt gtcggtgtgg gagttgaatc
      181 atgggtcgcg ctattttggc catctcaggt tcggcgattt cgttgctccc ccgtccgttt
      241 acggcgatcg gcagcatttt tgccggtttc tggcgtgaat tgacccgcga cgcggtccgt
      301 ccgtatcggc ccgaactgca ctatatgcgc ggtccggggc ccgcctggcg tgccaagcac
      361 ggccttccct cggtctgacc ccctatcttt tcggcttacc cgccctaaat tgtcgcgcct
      421 tacaggggcg cgcatgcagg gagtatcagg ggtatggttc gggctgcaat tctggatgat
      481 taccagaacg ttgcgatggg tttcgcggac tggtcgccgg tcggcaagga cgtcgagatc
      541 aaggtcttca acaagccgtt cggcagccag gacgaagcga tcaaggcgct gcaggggttt
      601 gccgttgtgg tcggcatgcg cgaacggacc cctttcccgc gcaaggtgat cgaggcactg
      661 cccgatctca agctgctgat cacgaccggg gcgcggaaca actcattcga catcaaggcc
      721 tgcgccgaac gcggcatcac agtttgcggc accggcgggg tcgggagccc gacgacgggc
      781 gtcgccttcg gcctgatgct ggaactcaca cgccgcatcg gtttcgagaa cgcgcggctg
      841 aaggccggcg cgccgtggca ggtgaccatc gggcgtgacc tcgagggtct cacgctcggc
      901 attctcggtc tgggcaagct cggccagcgc tcagcgacgg tcggcaaggc ctttggcatg
      961 aagaccatcg cctggagcca gaacctgacg gaagagaaag ccaaagcggc cggcgctggt
     1021 tacgtttcca aggacgatct tttccgcaac gcagacatcg tcaccatcca cctggtgctg
     1081 agcgatcgct cgcgcggcct ggtgggcgca aaagagctcg gcctgatgaa gaagtcggcc
     1141 tatctgatca acacgtcgcg cggtccgatc gtcgacgaga aagcgttaat tgccgcgttg
     1201 cagtcgaagt ccattgccgg agccggactt gacgtcttcg atatcgagcc gttgccgctc
     1261 gatcatccgt tccgcaagat ggacaatgtc gtcatcacgc cgcatctcgg ttatgtcagc
     1321 gagcagaact accgcaaatt ttttcccgac atcgtcgagg acatccgtgc ctggctcgat
     1381 ggcaagccgg tgcgggtgat cgaggcgaag tgacgcgtca ggccaagcga aaaagcctcg
     1441 ccgcgttgtt ataggcgacg tcggcgcgta tcgactcgtc gagcggcacc gcgtccatga
     1501 acgggccggc gccttccgcg tcgaaaggat agtcggccga gaacatcacg tgatcgcgcc
     1561 cgagcgcgcc gatcgagcag tgcaacggcg ggccgtcgta catgcccgac gtcgtgaccc
     1621 agacgttttc cttgatgtat tgcgacggcg gtttcggcag attccagccg tagggcttcg
     1681 accggctgtc gaagcgccag aggagaaagg gcaaggtttc gccgagatgc ccgagcagca
     1741 gcttggcttt gggatagcga tggaagacgc cgccggagat gacacgcaac gcatgcgagc
     1801 cggtctctac gccccagccc cacattggcc gcaccaacac attgtgacca gcgagcaccg
     1861 gcagcggtgc gaccgggtcc gcaggatgta tgtagaccgg cgcatcgaga gccgccgcac
     1921 gttcccagaa cgcatcatag gaacggtcat cgaggtattt gccgttcgtg tgaccgttga
     1981 tcatgacgcc gcaaaacttc agatcgcgca cgcaacgctc gagctcgtcg gcagcagcct
     2041 tggcgtcttg catggcaaga tgggcgaagc cggaatagcg gttgggccgc ttctgaattt
     2101 ccttggccag gaaatcattg gacgcacgtg cggcgcgtac tgcggtggcc gtatcacgct
     2161 ccgcctgcgc gccgggcccg gcgacggcca ggatcatccg cgcgatcccg tgttgttcca
     2221 tcatcattag acgctgctcg ccgaaatcag tcagggccgc ataaagctgt gccgtcttgg
     2281 ccggcgcgac gtcgaccatg gtcggccgcc aatacggctc cgtggctgga tcaagaaaat
     2341 gctcctcgag cgcgatcttt ttgaccatgc gttgcctccc agggccgatg gcccgcgtca
     2401 tcttttccct tttagctgta tcggattttg ggctaatgtg tcccacgatg caaggcggca
     2461 caagatcgcc ggcgagttgt gtaggcggga cgcgtcatga atcgtctggt taccaacgac
     2521 aaaagccagg tgcaggtgat cgcgcgggcc gccacgattc tgcgcgcact ggaagatgaa
     2581 aacagcggcc tgagccttgg gcagatagcc cagcgggtga acctcgcgcg ctcgaccgtg
     2641 caacgcatcg tggccgcact ggaaaccgaa aagctggtca tcgcggcgac gcccaacggc
     2701 cgcgtgcggc tcggtccgac aattctgcgc ctcgcggcat ccgtacgcag cgatttcgtt
     2761 gcgatggcac gtccgttcct tgaacgcctg tcggaagagc ttcacgaaac cgttgatctg
     2821 tcgacggtaa aaaaggatca ccttatcttc atcgaccagg tgatcggcgc acagcggctg
     2881 cgcgccgttt ctgcggtcgg cgatacgttt ccgctctact gcacggccaa cggcaaggcc
     2941 tatctcgcac agctcaccga caaagcgatc gagacgctga tcggacgcac ctacgaagca
     3001 cgaacgccca aaactcttac gcggttcgac gccctgctcg cggatctcaa gtcggcgcgg
     3061 cgcaacggcg tcgcctatga tcgagaggaa cacacattgg ggatctgcgc cgtcggtgtt
     3121 gcgatgcacg atccgctcgg caatgcgatt gcaatctccg tgccggtgcc aagccagcgt
     3181 tttgcaagcc ggcagacact ggttgccgag cgcctggtcg ctaccaaaaa tgccctggag
     3241 gcccacatga attcgtccgc tgcctgacag gcgccggaat catgacgaag taacgcatca
     3301 agggagggag ccgtggccaa aaaaattcgc ggcgtaatgt tcgaagaaaa cctgaaaaac
     3361 agcatggggc tggagttcta caacctgtcc catcgcttcg gctatcagtc gcctaattgg
     3421 ccatatttcg aagacgtcaa gatcgagcgt atccactaca tggcgaagtc gggcgtgctg
     3481 tcgcagcgca tcacgacctc gatgcataac acgacccata tcgacgcgcc ggcccacgtc
     3541 gttcaaggca cgccgttcat cgacgaggtg ccgctgccgc acttcttcgg ctcgggcatt
     3601 gtcgtgtcga tcccgaaaaa caaatgggaa ccgatcaccg gcgaagacct cgagaaggcc
     3661 tgcggcaaga tcgtgcggcc gcgcgatgtg gtgatcgtca ataccggctg gcacaaattc
     3721 tacgaagaca gcgaggatta tttctgtcgc gcgccgggct tcgtcccctc ggccggcgag
     3781 tggtttgtga aaaagaaggt caaggtggtc ggccacgata cgcaggcgaa tgaccatccg
     3841 ctcgcgaccg cgattggccc gcagcgcaac ggcccgctgc atccgcatct ggccgatgaa
     3901 taccgcgaat ggtccggcgg ccgcgactgg aaagatgact tccccgaatg ggagccggta
     3961 caccgcatcc tgttcaagaa cggcatcatg ggtatcgaga acgtcggcgg tgacatcgac
     4021 aaggtcaccg gaaaacgctg caccttcgcg tatttcccct ggaactggga gcgtggcgac
     4081 ggttgcattg tgcggctggt cgcaatcgcc gatccgaccg gaaaatttcg catcgagcgc
     4141 gggaggaaat tctaatgatc atccgcaacc tttccgatgc gaagccttac gaagcgccga
     4201 accaccgtgg ttatgaatct ctgcgcatct tcggcgcgga gatgggcgga tcgcaggcat
     4261 tggtcttcgg tatctcgcat ttccggccgg gcggcggcgc cggaccagac ggatcaccgc
     4321 cggagaaaat ctaccacgtg ctcaagggtg aactgaccgt gatcgccggc ggcaaagagc
     4381 aggtggcgaa gccgggcgac accgttttca tcgcccctaa cgagaaccgc gagatcgtca
     4441 accgcggcaa ggatgtctgc accatcctgg ttgcggtcgc ggcgcccaaa tagctgtcat
     4501 tccgggcccg cccgctgcgc acgcgtcccg gaatgacgag gagaagaaaa tgtccaacga
     4561 ctttctgaag aacccccagt cgctgttcga catcaagggt aagaccgcca tcgtcaccgg
     4621 cgcgtcgggt gctttcggcg cattggcggc gaaaacgttt gccggcgccg gcgccaatgt
     4681 ggttttggct gccagcaagg cggatgaatt gaagaaagtg gcggccgaat gcgagaagct
     4741 cggcggcaag accgaggtca tcgcgctacg gccgagttcg gaggaaaatt gcgagaagat
     4801 cgtcaaagcc gcagtcgaca agttcggcag agtcgacatc cttgtcgtcg cctccggcct
     4861 caacaaagtc tccaagattg tcgaccagaa agtcgaagat tttctcgacg taatggatgc
     4921 caacgtcaac cagtcatggt tgatggctcg cgcggccggc aagcaaatgc tcaagcaggg
     4981 ccaagaaggc aaacctggcg gcaaggtgat cctgacctcg tcggcgcgtg ggctgctcgg
     5041 tcatccggcc ggctacagcg cctattgtgc ctcaaagtcc gcggtcgacg gcatcaccaa
     5101 ggcactcggc tgcgagtggg gtgagacggg catcactgtg aacgcccttg gcccgacggt
     5161 cttccgttct ccgctgaccg catggatgta cgaggacacc gaacgtgcgc agacggtgcg
     5221 taaaggcttc ctggcccgcg tgccgaaagg ccggctcggc gagccggaag atctcgcggg
     5281 ccccctgctg tttctggcgt cgaaagcgtc ggatttctac accggccaca ttctctatgc
     5341 cgacggcggc tataccgcgg gataacgatg gccaaagcgc gcattgccgt gatcggtgcc
     5401 ggcctcatgg gccacggcat tgcgcaggtg ttcgcgctgg ccggccatga ggtgatgatt
     5461 accgatgccg tcacggcaaa tctcgacagt gccaagacac gaatcctcac taacctgaga
     5521 gatctcggcg acgaccagaa agcggtcgaa cacgtggcac cggtgcccga actgtcgcaa
     5581 gcggtgcgca acgccgacta tgtcgtcgag gcggttttgg aggatctgcc gctcaagcag
     5641 aagctgttcg tcgaaattga aaagcacgtg cggccgaaca cgatcctctc cagcaataca
     5701 tcggtgatcc cgattaccaa gatcatggaa ggtctcgcac accgcgagcg tgcacttggc
     5761 acccactggt ggaatccgcc ctatctcgtt ccgctggtcg aggtgatcga aacacaatgg
     5821 acgtcgccgc aagccgtcga cttcacgatg gcgcttcact ccgtagccgg aaagaagcct
     5881 gctcacgtga agaaggacgt gcccggcttc atcggcaacc ggctgcagca cgcgttgtgg
     5941 cgcgaagctg tcgcgcttgt cgagcaaggc atctgcgatg cagagacggt cgatgatgtg
     6001 atcaaatctg ccttcgggcg cagattggcc gtgctgggac cactcgaaaa cgccgatatg
     6061 gtgggtactg acctcacgct cgcaatccat aagacggtat tgccagacat cgacagccgt
     6121 ccgtcccctt cgccctatct cgaaaaactg gtcaaggacg gaaagcttgg cttcaagtcc
     6181 ggcgaaggct tccgcaagtg gagtccggag cagcaagctg cgctccgttc caaagtgctg
     6241 aaccatctta gaaacgcgcg cagcgaggac accaaaagcg cgcgttgaaa accagggagg
     6301 tgacaatgaa tgaccgccgc acgatttcac gccgcacgct ggtcaaatcc ggcct
//
LOCUS       DASSRL010000163         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1736728, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000163 DASSRL010000000
VERSION     DASSRL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736728"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1522
                     /gene="cysS"
                     /locus_tag="VFL49_03430"
     CDS             122..1522
                     /gene="cysS"
                     /locus_tag="VFL49_03430"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7911056.1"
                     /translation="MALRFYNTLTQQVEPFAPLHDNEVRMYTCGPTVYNYVHIGNLRT
                     FTFQDVLRRWLRARGYRLNHVMNITDVEDKIIKNALAQGKNIQEYTAVYTKAFFEDTA
                     VLRLEKPERIAEATAEINDMVAAVQKLEDKGYTYKSDGSVYYRISKFPEYGKLSHADF
                     GGMQAGARVDVDEYDKADARDFVLWKAKKEGEPSWPAPFGDGRPGWHIECSAMAMKYL
                     GETLDIHAGGVDLIFPHHENEIAQSEAITGKPFARFWLHSEHLNIDSQKMSKSLGNIY
                     TLRELLSMGHSPEAVRYLLASVPYRKKLNFTTDGLTAAAASIERLRNFKLRLDTEKFA
                     DGANEGVESRTRSAVEQFDGALDNDLNTAEALAAVFDWLREMNTAMDAGDVKSGNVKS
                     GLAFLARFDCVFDVLRPTSQGGGLSDEAVEELIAERTSAKKARDFARADAIRQELAGA
                     GVILEDTKEGVRWKRK"
     gene            1519..>2067
                     /locus_tag="VFL49_03435"
     CDS             1519..>2067
                     /locus_tag="VFL49_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET7911057.1"
                     /translation="MNINTKAVHAGDRKKSADHVPVTTPIYTATTFFYDSMDQLDRVF
                     GEEEQGFCYARYDNPTNSALEELVTSLENGHGSVACASGMAALQMAITGALIDRPKRV
                     VAANALYGATIKLLNNILEPFGVDTTYVDICDRDKAAQTIADRKPGCVVMETISNPLV
                     RVGEIDRLAEMAKAVGAALIVDN"
BASE COUNT          480 a          579 c          638 g          370 t
ORIGIN      
        1 agcagatcga tccacagttg tgcggcggca gttggcatgg acgccagaat accacccgca
       61 aaatcaatgg caaacagcct tggtcctcag cgccttgcta cgatgaaaaa tcaacctttg
      121 tatggccttg cgcttctata acaccctgac gcagcaggta gagccgttcg ccccgttgca
      181 cgacaacgag gtgcgtatgt acacctgcgg acccacggtg tataactacg tgcatatcgg
      241 caacctgagg acgttcacgt tccaggatgt gctgcggcga tggctgaggg cgcgcggcta
      301 ccggctgaac cacgtcatga acatcaccga cgtggaggac aaaatcatca agaatgcgct
      361 cgcccagggc aaaaatattc aggaatatac ggccgtatac accaaggcat tctttgaaga
      421 tacggccgtg ttgcggctgg aaaaaccgga gcggatcgcc gaagccaccg cggaaatcaa
      481 cgacatggtg gccgcggtgc agaagctgga agataaaggg tacacgtaca agagcgacgg
      541 gtccgtttat taccgcatct cgaaatttcc ggaatacggc aagctgtccc atgccgattt
      601 cggcggaatg caggcgggcg cgcgggtgga tgtagacgaa tacgacaagg ccgatgcccg
      661 cgacttcgtg ctgtggaagg cgaagaagga aggcgaaccg agctggcccg ctccattcgg
      721 cgacgggcgg ccgggctggc acatcgaatg ctccgcgatg gccatgaaat acctgggcga
      781 aacgctcgat attcacgccg ggggcgtcga tctgatcttt ccgcaccacg aaaacgagat
      841 cgcgcaatcg gaggcgatca cgggcaagcc cttcgcccgg ttctggctgc actccgagca
      901 cctgaacatc gattcgcaaa agatgtccaa gtcgctcggt aatatctata cgctgcgcga
      961 gctgctttcg atgggccact ctccagaagc ggtgcgatac ctgctcgcct cggttcccta
     1021 tcgcaagaag ttgaacttca cgacggacgg cctcaccgcg gcggcggcat ccattgagag
     1081 gcttcgcaat ttcaagctgc gcctggatac cgagaagttt gccgacggcg ccaacgaagg
     1141 cgtcgaatcc cgcacgcggt ccgccgtcga gcagttcgat ggagcgctgg ataacgatct
     1201 caataccgcc gaggcgctgg cggcagtgtt cgactggctg cgcgagatga atacggcgat
     1261 ggatgccggc gatgtcaaat cgggtaatgt gaaatcaggg ctggcgtttc ttgcccggtt
     1321 cgattgcgta tttgacgtac tgcgaccgac gtcccagggc ggcggtctca gcgacgaagc
     1381 agtcgaagag ttgattgcgg aaaggaccag cgcgaagaag gcaagagact tcgccagggc
     1441 cgacgcgatc cggcaggaac tggccggcgc gggcgtcatt cttgaagata cgaaggaggg
     1501 cgtccgctgg aaaaggaaat gaacatcaac accaaggcgg tgcacgccgg cgatcgtaaa
     1561 aaatcggccg atcacgtgcc ggtgaccacg ccgatctaca cggctaccac tttcttctac
     1621 gattcgatgg atcagctcga ccgagtcttc ggagaggaag agcagggatt ctgttacgcg
     1681 cgctacgaca atccgactaa ctccgcgctc gaagagctgg tgacatctct cgagaacggg
     1741 cacggctcgg tggcctgcgc atcggggatg gcggcgctgc agatggcaat caccggcgcc
     1801 ctgatcgacc gcccgaagcg ggtagtagcc gcaaacgctc tgtacggggc cacaatcaag
     1861 ctgctgaaca acatcctcga gccgttcggc gtggacacga cgtacgtgga tatttgcgat
     1921 cgcgataagg ccgctcagac gatcgccgat cgtaagcccg gctgcgtggt tatggaaact
     1981 atttccaatc cgctggtgcg agtcggcgaa atcgaccgcc tggcggaaat ggcgaaggca
     2041 gtgggcgccg cgcttatcgt ggataat
//
LOCUS       DASSRL010000164         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_599405, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000164 DASSRL010000000
VERSION     DASSRL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599405"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1070
                     /locus_tag="VFL49_03440"
     CDS             258..1070
                     /locus_tag="VFL49_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET7911058.1"
                     /translation="MSISRGRLPPILAQKHYTTPSAFTPENLLREARRQKRITGSSIP
                     AICVLDPDGDIVRRLLAAGEARIEPAWACYHTQLCTFTRAGIDFGIVGCAVGASFAVL
                     IAEEMFASGCKLLISMTSSGQIVPVRPPPYFVVIESALRDEGTSYHYMAPSEYSRADA
                     ALIAALDRAFDGFRVPILIGATWTTDAPFRETQPAIDAMCEKNLLAVEMEAAALYAFA
                     HMRQKPVLCFAHVTNQMGRIDGDFEKGEADGSKDALDLIAIVADRLRRRLSL"
     gene            1067..1762
                     /locus_tag="VFL49_03445"
     CDS             1067..1762
                     /locus_tag="VFL49_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET7911059.1"
                     /translation="MSVAAIGIMCKAPQPGHTKTRLAKSIGAVDASELAACFLRDVAA
                     AVEAVPEGLGRRGYGVYAPAGAERKLRALFPEKFGLMLQTGDDFGDVLFGAARALLDA
                     GHDCVLLVNGDSPTLPPSFLVQAIEALRSPGERMVLGPASDGGYYLIGLKSLHRELFT
                     RIAWGTDTVAHSTRERAAEIGLAATMLPEWYDIDDLETLDWLRDELTGNSMRFRGGGP
                     AHATRAFLTSGGK"
     gene            1840..3132
                     /locus_tag="VFL49_03450"
     CDS             1840..3132
                     /locus_tag="VFL49_03450"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7911060.1"
                     /translation="MAGLGAISAGLTVVAPFAFRAGGDNAFIAPAVAASLLALAATHV
                     AERIPTARALWLILGLGIALRGYVLFFDPLLSSDIYRYVWDGKVQAAGINPYRYVPAQ
                     DVLAHLRDAAIFPQINRPDYAVTIYPPVAEFFFLIATRIGENVNAMRLGLLACEAVSV
                     TLIILFLRRLDLPATRVVAYFWHPLPIWEIANNGHVDALAVALMLLGLWVALTGRALR
                     GAVLITLAVLVKPYVAPVLAVIWRAWDWKVPLVVITAIALCYLPYLSAGWGIFGFLTQ
                     GYLAEEGITSGDKLWLLSVWRLAFGTIRGDVAAYAAVSVAILLFTALSVARRRNRTLA
                     SALSDANTLLLVALLLLSPNYPWYFLAITPFLALCGNAPTWAVSIGAILLTNEVDGDF
                     SIPLLTAKSILFGGFLIACIWSARGRYLKQVTYAGRSQ"
     gene            3129..4208
                     /locus_tag="VFL49_03455"
     CDS             3129..4208
                     /locus_tag="VFL49_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7911061.1"
                     /translation="MTHSSGGPLVGTRIDPRRYHESVRRERTETAGRPPVCLYLEVTN
                     RCNLLCTTCPRTYEDLEPPADMSWELFTSIVDQVPDLARAVLHGVGEPMLVANLPRMV
                     RYLKDRGTYVLFNSNGTVLSERNGRALIAAELDELRVSLDASNRESFKTVRGRDYFGR
                     IVRNVRAFRELQEREGHLKPQVSIWLTGLKETVAELPAFVQLAAEIGVREVYLQRLVF
                     FDQSAIGKARPDQALFERLTREEATYLKQAEDLASSLGIAFSASGAASEPGLSLKRTD
                     ESSPWSLCRRPWSLMYFTANGRALPCCIAPFSQHGYDNYTLGNATQQTLRDIWNGPAY
                     REFRKALLSDKPPTACASCGLRWSL"
BASE COUNT          739 a         1313 c         1295 g          922 t
ORIGIN      
        1 atcgccacac tccatcttcc ctgtcaggat agagtgttgc ggcgaccggt tgagaccacc
       61 accctcagtg atgcgatcct gccggggcac agccgatccg cgcgttcaac tcgcgaatca
      121 catatccgtg tggttgccgg gcatttgacc cgaaacgcgg cgccgccgca tattattcca
      181 tccagcctcg acgaagcttg tcggacgtcg ctgagaacgc tgccgaaacg tcagttcacg
      241 cctcaaaggt cggtattatg tcgataagcc gcggccgact tccgccgatc ctcgcgcaaa
      301 aacactacac cacgccctct gcatttacgc cggaaaatct gctgcgcgag gcgcgccggc
      361 agaagcgaat taccggcagc agcattccgg caatctgtgt tctcgatccc gacggcgata
      421 tcgtccgccg tctgctggct gcgggcgagg cccggattga gcccgcatgg gcctgctatc
      481 acacgcaatt atgtactttc acccgcgccg ggatcgattt cggaattgtc ggctgcgccg
      541 ttggcgcttc attcgccgtg ctcattgccg aggaaatgtt tgcgtcgggt tgcaagttgc
      601 tgatcagcat gacctcttcc ggacaaatcg taccagtacg cccgccgccg tattttgttg
      661 tcatcgagag cgcgctacgc gacgaaggca ccagttatca ctacatggcg ccctccgaat
      721 acagccgagc cgacgccgcg ttgatcgcgg cactcgaccg cgcattcgat ggattccgcg
      781 tgcctatcct gataggtgcg acctggacaa ccgacgcgcc gttccgggag acgcagccgg
      841 ctattgacgc catgtgtgaa aagaatctgc tggcggttga gatggaggcg gccgcgctct
      901 atgccttcgc ccacatgcga cagaagccgg ttttgtgttt cgctcacgtc acaaaccaga
      961 tgggccgtat cgatggcgat ttcgagaagg gtgaggccga cggcagcaaa gatgcgctgg
     1021 acctgatcgc gatcgtagcc gatcgacttc ggcgccggct ctcgctgtga gtgttgccgc
     1081 gatcggcatc atgtgcaagg cgccccaacc ggggcacaca aaaacgcgac ttgcgaagtc
     1141 gatcggcgcg gttgatgcgt ccgaattggc cgcctgcttt ctgcgtgacg ttgccgccgc
     1201 agtcgaagca gtccccgaag gacttggcag gcgcggctac ggcgtctacg ccccggcggg
     1261 agccgagcgc aagctacgcg cgctgttccc ggaaaagttt gggctcatgt tgcagacagg
     1321 ggatgatttc ggggatgtgc ttttcggtgc cgcccgggct ctgttagatg caggacacga
     1381 ctgcgtattg ctcgtcaacg gtgacagtcc aacgctgcct ccctcttttc tggtgcaagc
     1441 cattgaggcg ctgcgcagcc cgggcgagcg gatggtgctc gggcctgcca gcgatggcgg
     1501 gtattacctc atcggcctga aaagcctgca cagggaacta ttcacgcgga ttgcctgggg
     1561 aacagatacc gtggcccaca gcacgcgcga gcgcgcagcc gaaatcgggc ttgcggccac
     1621 catgcttcca gaatggtacg atatcgacga tctggaaaca ctggactggc tgcgggacga
     1681 gctgaccggc aattcaatgc gctttcgtgg cggaggccct gcccacgcga cccgcgcctt
     1741 tcttacctca ggcggcaagt gaatctcatg ccgaattcgg ttgtggggca aatcactcga
     1801 gcaggatgct ccgccggaaa tgtaccgtcg ctttatccat tggctggtct cggtgcgatt
     1861 tcggccggcc tgaccgtggt ggcgcctttt gcgttccggg ccggcggcga caatgccttt
     1921 attgccccgg ccgttgctgc aagcctgctg gcgcttgcag cgacgcatgt cgccgagcgc
     1981 atcccgacgg cgcgtgcgct ttggttgatc ctcgggcttg ggattgcgct gcggggctat
     2041 gtgttgtttt tcgatccgct gttgtcgagc gatatctatc gttacgtctg ggatggcaaa
     2101 gttcaggcgg ccggcatcaa tccctatcgc tatgtccccg cccaggatgt tctggcgcat
     2161 ctgcgcgatg cggcaatctt tccccagatc aatcggcccg attatgccgt caccatctac
     2221 ccgcccgtcg ccgaattctt ctttctgatc gccacgcgta tcggcgaaaa cgtgaacgcc
     2281 atgcgccttg gcttgcttgc ctgcgaagcg gtgagcgtca cattgatcat actcttcttg
     2341 cgccgcctgg atctgccggc gacccgcgtt gttgcatatt tctggcaccc gctcccaatc
     2401 tgggagattg ccaacaatgg tcacgtcgat gccctggcgg tcgcgctgat gctgcttggt
     2461 ctgtgggtcg cgctgaccgg tcgcgcactg cgtggtgccg ttttgatcac cctcgcggta
     2521 ctcgtgaagc catatgttgc gccggtcttg gctgtgatct ggcgcgcgtg ggactggaag
     2581 gtcccgctcg tcgtgatcac cgcaatcgcg ctttgttacc ttccctacct ttccgcgggc
     2641 tggggaatct tcgggttcct gacccaaggt tacctggcgg aagagggcat tacgtccggc
     2701 gataagttgt ggctgctatc ggtctggcgt ctcgctttcg ggacgatccg gggcgatgtt
     2761 gccgcctatg ccgcagtgtc ggtggcaatt ctgctgttca cggcactttc cgttgcccgc
     2821 cgccggaacc ggacgctcgc gtccgccctt tccgacgcca atacattatt gctggtcgcg
     2881 ctgctgctgc tgtcgccaaa ctatccatgg tacttcctgg caatcacgcc gtttcttgca
     2941 ctttgcggta atgcaccgac ctgggcggtt tcaatcggag ctatcctgct gacgaacgaa
     3001 gtcgatggag atttttccat tccgttgctg accgccaagt ccatcctgtt tggcggattt
     3061 cttattgcgt gcatctggtc ggcgcgcggc agatatctca agcaggtcac gtacgcggga
     3121 cggtcacaat gactcattcc agcggtggtc cgcttgttgg cacgcgaatc gacccgcgcc
     3181 gctatcatga atcggtccgc cgcgagcgca ctgaaacggc cggccggccg ccggtctgcc
     3241 tttatctgga agtgaccaat cgctgcaatt tgctgtgcac cacctgcccg cgcacctacg
     3301 aggacctcga gccgccggca gatatgagct gggagttgtt cacatcgatt gttgatcagg
     3361 tgcccgatct tgcgcgggcc gtactgcatg gcgtcggcga gccgatgctg gtggcgaacc
     3421 tcccacgcat ggtgagatat ctgaaagatc gcggcaccta cgtgctgttc aacagcaacg
     3481 ggaccgtcct cagcgaacga aacggccgcg cgttgatcgc cgccgagctc gatgagctgc
     3541 gcgtttcgct cgatgcgtcg aaccgcgaga gtttcaagac cgttcgtgga cgcgattact
     3601 tcgggcgcat tgtgcgcaat gttcgggcat ttcgcgagct gcaggagcgc gagggacacc
     3661 tgaagccgca agtctccatc tggcttacgg gcctgaagga gacggtcgcg gaattgccgg
     3721 ctttcgtgca gctggcggcg gaaatcggcg tcagggaagt ctatctgcag agactggtgt
     3781 tcttcgatca atcggcaatc ggcaaggcgc ggccggacca ggcgctgttc gagcgcctga
     3841 cccgcgaaga ggcaacctat ctcaagcaag ccgaggacct tgcgagttcg ctcggcatcg
     3901 ccttcagcgc gtccggcgcg gcgtccgagc ctggcctgag cctgaaacga accgacgaaa
     3961 gctcgccgtg gtcactctgc cgacggccat ggtcgctgat gtatttcacc gccaatggcc
     4021 gggcgttgcc ttgctgtatc gcgccttttt cacagcatgg ttatgacaac tacactctcg
     4081 gcaatgcgac gcagcaaacc ttgcgcgaca tctggaacgg gcccgcctat cgggaatttc
     4141 gcaaggctct cctgtccgac aagccgccga cggcctgcgc cagctgcgga ctgcgctgga
     4201 gcctgtgagc gacgttgcgt gcaaaagtaa tgcgccgcga cctctccgcc cggccgtcgt
     4261 gtcggcggt
//
LOCUS       DASSRL010000165         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1719238, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000165 DASSRL010000000
VERSION     DASSRL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719238"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..388
                     /locus_tag="VFL49_03460"
     CDS             149..388
                     /locus_tag="VFL49_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7911062.1"
                     /translation="MSDIGERVKKIVVEHLGVEPDKVNDQASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDAVKFLEKNAKS"
     gene            483..1748
                     /gene="fabF"
                     /locus_tag="VFL49_03465"
     CDS             483..1748
                     /gene="fabF"
                     /locus_tag="VFL49_03465"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7911063.1"
                     /translation="MRRVVVTGMGMLTPLGCGVEATWARLLKGESGAKKIDSFDVSDI
                     ACKIACVIPRGDGAGATYNSDQWMEPKEQRKVDEFIVFAMCAARQALDDAGWHPKSHE
                     EQITTGVMIGSGIGGIEGIAQTAIALHERGPRRVSPFFIPGRIINLASGFVSIAFGLK
                     GPNHAVVTACSTGAHAIGDAARLVALGDAEVMVAGGAESPVNRISLAGFSALRALSTN
                     FNDDPTRASRPYDKDRDGFVMGEGAGAVVLESLEHAKARGAKIYAELIGYGLSGDAYH
                     ITAPAPDGDGALRCMSAAIRRAGISAVDIDYINAHGTSTPLGDEIELGAVQKLVGNAA
                     SRMSMSSTKSCIGHLLGAAGAVEAIFSILAIRDRVAPPTINLDNPSVDTPIDLVPHQA
                     RKRDVDTALSNSFGFGGTNASLVFRRYTN"
     gene            1935..3002
                     /gene="mltG"
                     /locus_tag="VFL49_03470"
     CDS             1935..3002
                     /gene="mltG"
                     /locus_tag="VFL49_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7911064.1"
                     /translation="MPRSPRAALEPERVPMPSRRSSRARHPIVVIGNAIFTALIVISI
                     AVGAAMFFGKQRFEAAGPLAEDKVVNIPRGLGIRDIADVLQREGVIDQPYIFMGGVIA
                     LKARGDLKYGEYQFGKHASVADVVDTIIEGKVVQHAFTVPEGLTSEQIVARLLENTAL
                     SGQIREIPREGTLLPETYRFTRGMTREQIIQRMQQAHRRVLQDVWDHRMPDLPVKTPE
                     QLVTLASIVEKETARPDERTRVAAVFVNRLKAKMRLQSDPTIIYGLTGGKGSLGRPIL
                     KSEIEQPTPYNTYVIDGLPPGPIANPGRASLEAAANPARTKELYFVADGSGGHLFSDN
                     YTEHQKNVARLRGIEHVNGKQ"
     gene            3084..3971
                     /locus_tag="VFL49_03475"
     CDS             3084..3971
                     /locus_tag="VFL49_03475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET7911065.1"
                     /translation="MALSSMTGFARSHGVAGAYAWAWEIKSVNAKGLDLRFRFPPGWD
                     AVEVPARARGTEKLSRGTVYANLTVERKGIQPTVRVNEPVLGAVLSALQRLSAKVKAS
                     PPTLDGILSLKGVIEVTEEDESEEDHRAAEAAIVSGFERALTALLDMRQAEGQTLGRL
                     LSTRLDEITALSTRAEKAPGRKPEAIRARLAEQVSALLEASERFDSERLYQEAVLLAA
                     KADIREELDRLASHAAQAKKLLADGGAIGRKLDFLAQELHRESNTLTAKANDLELTNI
                     GLELKSTVEQFREQVQNLE"
BASE COUNT          885 a         1123 c         1233 g          766 t
ORIGIN      
        1 tttcggcccg gctggagcgg ctaagagcgg actaatgttc cgcaattggg gatcgtctca
       61 tcaccgtcaa ccacgacgag gccatagcgc gatggttgcg aactgagcct ggctcgcagc
      121 ccaattcata aagcacgagg ttggaacgat gagtgacatt ggcgagcggg tgaaaaagat
      181 cgtcgtggag cacctgggcg tagaacccga caaggtgaac gatcaggcca gctttatcga
      241 tgatcttggc gccgacagcc tcgacaccgt cgagctcgtg atggcctttg aagaagaatt
      301 cggctgtgaa atccccgatg atgccgctga aaccattctg accgttggtg acgcggttaa
      361 atttctcgaa aaaaacgcta aaagctgagc gcgctgcacg cagctgctcg attgttttgc
      421 tcgtcaatgg ctttgagctg tacgcggcgc ggcagcggca atagatttgc ccggggccgg
      481 ccatgagacg tgtcgtcgtt accggaatgg ggatgctcac gccgctgggt tgcggcgtcg
      541 aggcaacctg ggcgcggctg ctcaaaggcg aaagcggcgc aaagaagatc gacagcttcg
      601 acgtttccga cattgcctgc aagattgcct gcgtcatccc gcgcggcgac ggagccggtg
      661 ctacttacaa ttccgaccag tggatggaac cgaaagagca acgcaaggtc gatgaattta
      721 tcgtcttcgc gatgtgcgcg gcgcgtcagg cgctcgatga tgccgggtgg caccccaaaa
      781 gccatgagga gcagatcacg acgggcgtaa tgatcggatc gggtatcggc ggaatcgaag
      841 gaatagctca aacggcgatt gcgctccacg aacgcggccc gcggcgcgtg tcaccgttct
      901 ttattcccgg gcgcatcatc aatcttgcat ccggcttcgt gtcgatcgca ttcggcctta
      961 agggtccgaa ccacgcggtg gtcaccgcgt gctcgaccgg cgctcacgcc atcggagatg
     1021 cggcaaggct tgtcgcgctc ggtgatgccg aagtcatggt agcaggcggt gcagaatcgc
     1081 cggtcaaccg tatttcgctg gcagggtttt ctgctttgcg ggcactttca acaaatttca
     1141 acgatgatcc gacacgcgcg tcacgacctt atgacaaaga tcgcgacggt ttcgtcatgg
     1201 gcgaaggggc cggtgccgtg gtgctggagt ctcttgagca tgcaaaggcg cgcggcgcca
     1261 agatttacgc ggagctgata ggttacggat tgtctggcga cgcctatcac atcaccgcgc
     1321 cggcgccaga cggcgatggc gcgctacgct gcatgagcgc ggcgatccgc cgcgcgggta
     1381 tctcggcagt agatatcgac tacatcaacg cgcacggcac ctcgacccca ctgggagatg
     1441 aaatcgagct cggtgcggtg caaaaactgg tcggcaatgc ggccagccgc atgtcgatgt
     1501 cttcgaccaa atcgtgtatc ggacatttgc ttggtgcggc gggtgctgtc gaagcgatat
     1561 tctcgattct cgcgatccgc gaccgagttg cgccgccgac gatcaatctc gacaatccgt
     1621 cggtcgacac tccgatcgat ttggtgccac accaggcgcg caagcgtgac gtcgatacgg
     1681 cactctcgaa ttctttcggc ttcggcggaa caaatgcatc tttggttttc cgccgatata
     1741 caaactaaaa aagcgtcgac aaagtccccc agctttcgcc ataatttttt ctaacgtgcg
     1801 aaattcgctg gtgtttcgtc ggcgaagcga tgccagagga acgaatggcg gtagcggctg
     1861 gtggagtgga ctaggtgaat tccaatttcc cgccgggaga tagtggcaat ggcttggcgg
     1921 ccgtaaagcc ggcaatgccg cgcagcccgc gcgccgcgct cgagccggaa cgtgtgccaa
     1981 tgccgagccg gcgctccagc cgcgcacgtc atccgatcgt cgtaatcggg aatgcaattt
     2041 ttaccgcgtt gatcgtgatt tcaatcgcgg tcggcgccgc aatgtttttc ggtaagcaac
     2101 gcttcgaggc cgccggaccg ctcgccgaag acaaggtcgt caatattcca cgcggcctgg
     2161 gaatccgtga catagctgac gtgctgcaac gcgagggcgt gattgatcaa ccttacatct
     2221 tcatgggtgg cgtcatcgct ctcaaggcgc gtggagatct taaatacgga gaatatcaat
     2281 ttggcaagca cgcgagcgtc gccgacgtgg tcgacacgat tattgaaggc aaagtggtgc
     2341 agcacgcttt cacggtcccg gagggactga catccgaaca gattgttgcg cgccttctcg
     2401 aaaacactgc gctcagcgga caaatcagag agattccgcg cgaaggcacg ctgttaccgg
     2461 aaacataccg gttcacgcgc ggaatgacgc gcgagcagat catccagcgc atgcagcaag
     2521 cacaccggcg ggtgttgcag gatgtctggg accacaggat gcccgatctg ccggtcaaga
     2581 cgccggagca actggtgacg cttgcgtcga tcgtggaaaa ggaaactgcc cgacctgacg
     2641 agcgaacacg ggtggcagcg gtttttgtaa accgtctcaa ggccaaaatg cgactgcagt
     2701 cagatccgac cattatttac ggactgacag gggggaaggg atcgctcggc cgtccgattc
     2761 taaagagcga aatcgaacag ccgacaccat acaatacgta tgttatcgac gggctgccgc
     2821 cggggccgat cgcaaatccg ggacgtgcct cattggaggc tgcggcgaat ccggcgcgca
     2881 ccaaggaact ctatttcgtc gccgatggca gcggcggcca tctgttctcg gataactaca
     2941 ccgagcatca aaaaaacgtc gcgcgattgc ggggcataga gcatgtcaac ggcaagcagt
     3001 gagttgtccg cccttgtgac acgtggtgcg cgcagctaag gtcgccgcac ttcgacgaag
     3061 catcaacgcg agattcctaa gccatggctc tgtcgagcat gaccggtttt gctcgaagcc
     3121 acggcgtcgc gggcgcttat gcgtgggcat gggaaatcaa gtccgtgaat gccaaaggcc
     3181 tggacctgcg ttttcgtttt cccccgggat gggatgcagt ggaagtgccc gcaagggccc
     3241 gcgggacgga aaaactctct cgcggcacag tgtacgccaa cctcactgtc gagcgtaaag
     3301 gcatccagcc taccgtaaga gtgaatgaac ccgtgcttgg cgcggtgctt tcggcgcttc
     3361 agaggctttc cgccaaggta aaggcgtcac cgccaacact ggacggaatt ctgtcgctca
     3421 agggtgtgat cgaagttacc gaagaggacg agagcgaaga ggatcatcgt gccgccgagg
     3481 cggccattgt ctccggattc gaacgggcac tgacggcgtt gctcgacatg cgacaggccg
     3541 aaggacagac tctgggacgg ttgctatcga cgcggcttga tgaaattacg gcactttcca
     3601 cacgggcgga gaaagcaccc gggcgcaagc ctgaagcaat cagggcacgc ctcgccgaac
     3661 aggtgtcagc gctgcttgag gcgtcagagc gcttcgactc ggaacggctc taccaggaag
     3721 cggttctgct tgcggccaaa gccgatatcc gtgaagagct tgatcgcctt gcctcgcatg
     3781 cagcccaggc aaagaagctt ctcgccgacg gcggcgcgat cggccgcaag ctcgactttc
     3841 tggcgcagga attgcaccgc gaatcaaata cgttgacggc caaggccaac gaccttgagc
     3901 tcaccaacat cggcctcgag ctcaaaagca ctgtcgagca gtttcgcgag caggtgcaga
     3961 acctggagtg aacacgcgat ggcggcccag caaaaacgag acgtcat
//
LOCUS       DASSRL010000166         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1474264, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000166 DASSRL010000000
VERSION     DASSRL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474264"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2163
                     /gene="pflB"
                     /locus_tag="VFL49_03480"
     CDS             <1..2163
                     /gene="pflB"
                     /locus_tag="VFL49_03480"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET7911066.1"
                     /translation="QNVTSYTGDEKFLAGPSKRTKAVWEKLQPYFQEERKKGVLAVDA
                     KTPSTMLAHKPGFIDRNNEVVVGLQTDQPFKRAIFPYGGLRMVEAGLKAAKFDADPQV
                     HEAFTKYRKSHNDGVFDAYTPEIMRCRKSGIITGLPDAYGRGRIIGDYRRVALYGIDR
                     LLEAKQAERAQIDDMWPTDDVIRQREELAEQVRALNDLAAMAKQYGFDISQPATDARE
                     AFQWTYFAYLGAIKEANGAAMSIGRISSFLDVYIERDLREGTLDEPGAQELWDQLVQK
                     LRIVRFLRTPDYDALFSGDPYWATECVGGMDVDGRTLVTKSSYRMLHTLNNLGPAPEP
                     NITVLWSKHMPEEFKKFCIKVSRDTSSLQYENDDLMRPFWGDDYGIACCVSAMRLGKQ
                     MQFFGARVNLAKALLYAINGGRDEVSGEQIAPATPPVAGEFLSYDEVMKKFDITMDWL
                     ARTYVHAMNCIHYMHDKYFYERLEMALHDRDILRTMAFGIAGLSVVADSLAAMKYGKI
                     RVIRDDTGLAVDYKNESKAPVPQFGNNDDRVDSIASDLVTRFMQKIRKHPTYRNATHT
                     QSVLTITSNVVYGKATGNTPDGRRKGEPFGPGANPMHGRDSHGWLASCLSVAKLPYKD
                     AQDGISYTVSVAPQKAHLSEKELVDGATKAFDTFFDHGGFHMNLNVIDKDMLEDAMKN
                     PDKYPQLTIRVSGYAVNFVRLTREQQLDVISRTFHGQI"
     gene            2178..>2903
                     /gene="pflA"
                     /locus_tag="VFL49_03485"
     CDS             2178..>2903
                     /gene="pflA"
                     /locus_tag="VFL49_03485"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HET7911067.1"
                     /translation="MSQLRQLEAGSRHDLRVGNSPDAPDESQFKEAEGAFGYAHSYET
                     SSRYDGPGVRVVLFVSGCLLRCSYCHNPDTWHLKDGTYVSAQHVLERLSGFARALRSM
                     DGGLTISGGEPMVQLAFTKRILAGAKKMGLHTAIETSGFLGDRADDSYLSVLDLVLLD
                     IKSSNPDTYRAVTGRDLAPTLRFAERLASIGKRVWVRFTLVPGFTDDPANVDGIAKFV
                     APMKNVEWVEIQPFHQMGAFKWKA"
BASE COUNT          633 a          868 c          855 g          547 t
ORIGIN      
        1 cagaacgtca cctcctacac gggcgatgag aaatttctgg caggcccttc caagcgcacc
       61 aaggccgttt gggaaaagct gcagccatac tttcaggaag agagaaaaaa gggcgtgctc
      121 gccgttgatg caaaaacacc atcgacgatg ctggcgcaca agccaggctt tatcgatcgc
      181 aacaacgagg tcgttgtcgg tcttcagacg gaccagccct tcaagcgcgc gatatttccc
      241 tacggcggcc tgcgcatggt cgaggcgggc ctcaaggctg ccaagttcga cgccgatccg
      301 caggtccatg aggcgttcac gaaataccgc aagagtcaca acgacggcgt tttcgacgcc
      361 tacacgccgg aaatcatgag atgccggaag tcgggcatca ttaccggcct gcccgacgca
      421 tacgggcgcg gccgtatcat cggcgactat cgccgcgtgg cactttacgg aattgatcgc
      481 ctgctcgagg ccaagcaggc ggagcgggcg cagattgacg atatgtggcc gacggacgac
      541 gtcatccgcc agcgggagga actggctgag caggtccgcg cgctgaacga tctggctgca
      601 atggcgaagc aatacggatt cgacatctct cagcccgcca cagacgcccg agaagccttt
      661 cagtggacct attttgcgta tctcggcgct atcaaggagg caaacggcgc ggcaatgtcg
      721 atcggccgca tctcgagttt cctcgatgtc tatatcgaac gcgatttgcg cgaaggcacg
      781 cttgatgagc ctggtgctca ggagctctgg gaccagctcg tacagaagct gcggatcgtg
      841 cggttcctcc gcactcccga ctacgacgcc ctgttcagcg gcgatcccta ctgggcaacc
      901 gaatgcgtgg gcggcatgga cgtggacggg cgaacgctcg tgaccaaaag cagctatcgc
      961 atgctgcaca ccttgaacaa tctgggcccg gcgcccgaac cgaatattac cgtgctgtgg
     1021 tccaagcaca tgcccgaaga gttcaagaaa ttctgcatca aggtcagccg cgatacgtct
     1081 tccctgcaat atgaaaatga cgatctgatg cggcccttct ggggagacga ttacggcatc
     1141 gcgtgctgcg tttcggcgat gcggctcggc aagcagatgc agtttttcgg cgcgcgtgtg
     1201 aacctcgcca aagcgctgct gtatgccatc aatggcgggc gtgacgaagt ttccggcgaa
     1261 cagattgcgc cggcaacgcc gcccgtcgcc ggcgagtttc tcagttatga cgaggtgatg
     1321 aaaaaatttg acatcaccat ggactggctc gcgcgcacct atgtgcacgc aatgaattgc
     1381 attcactaca tgcacgacaa gtatttctac gagcgtctcg agatggcgct gcatgaccgt
     1441 gatatcctgc ggacgatggc gttcggaatt gcgggcctgt ccgtcgtcgc cgacagcctt
     1501 gccgccatga agtacgggaa gatccgcgtc attcgcgacg acaccggcct ggccgtcgac
     1561 tacaaaaacg aaagcaaggc accggttccg cagttcggca acaacgacga ccgcgtcgac
     1621 agtattgctt cggatcttgt cacacgattc atgcagaaga tccgcaagca tccgacctat
     1681 cggaatgcga cgcacacgca aagcgttctg acaatcacct caaacgtcgt ttacggaaag
     1741 gctaccggaa atacgccgga cggacgccgc aagggcgagc cgtttggccc gggggccaac
     1801 cccatgcacg gccgagacag ccacggctgg cttgcctcct gcctgtcggt cgccaagctc
     1861 ccctacaagg acgcgcagga cggcatcagc tacaccgtct cggtcgcacc gcaaaaggcg
     1921 cacttgtctg aaaaggagct ggtagacgga gcgaccaagg ccttcgacac atttttcgat
     1981 cacggtggat tccacatgaa tctgaacgtg atcgacaagg acatgctcga agacgcaatg
     2041 aagaatccgg acaagtaccc gcaattgacg atccgtgtgt ccggctatgc ggtcaacttc
     2101 gtgcggctaa cgcgcgagca acagctggat gtgatcagcc ggacgttcca cggccagatc
     2161 tgaagtgggg atgtgcgatg tcacagctcc gacagctcga ggccggcagc cgccacgacc
     2221 tgcgcgtcgg caattcgccg gacgcgccgg atgaaagtca attcaaggaa gccgagggcg
     2281 ccttcggcta cgcgcactcc tacgagacat cgtcccgcta cgacgggcct ggcgtgcgcg
     2341 tcgtcctgtt cgtgtcgggt tgcctcttgc ggtgctcgta ctgtcacaac cccgatacgt
     2401 ggcatctcaa agacggcact tatgtctccg cccagcacgt gcttgagcgt ctaagcggat
     2461 ttgcgcgcgc tttgcgcagc atggacggcg gcctgaccat atcggggggc gagccgatgg
     2521 tgcaattggc ctttaccaag cgaattctgg cgggcgccaa gaaaatgggc ctgcacacgg
     2581 cgatcgaaac ttcgggattt ctgggcgacc gagccgatga ctcatacctt tcggtcctgg
     2641 atctcgtcct tctcgatatc aaaagctcga acccggacac ctatcgtgcc gttaccggcc
     2701 gcgaccttgc gccaaccttg cgcttcgcgg aacgcctggc ctccatcggc aagcgcgtat
     2761 gggttcgctt cacactcgtg ccgggcttca cggacgatcc cgctaacgtc gatggaatcg
     2821 cgaaatttgt cgctccgatg aagaacgtcg agtgggtgga gattcagccg tttcaccaga
     2881 tgggcgcctt caagtggaag gcg
//
LOCUS       DASSRL010000167         7374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_266847, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000167 DASSRL010000000
VERSION     DASSRL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266847"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..485
                     /locus_tag="VFL49_03490"
     CDS             210..485
                     /locus_tag="VFL49_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911068.1"
                     /translation="MAYGINIPANVRRAAYFVDKMLKGAKASDLPVELPTKVELVINL
                     KTAKALGTQCRQESSSASCLSKTSNCFSERPPQKAVFLFVCVQCPLL"
     gene            complement(734..1225)
                     /locus_tag="VFL49_03495"
     CDS             complement(734..1225)
                     /locus_tag="VFL49_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7911069.1"
                     /translation="MSDAKHVSKRAVPLRKTPQKRTCAPVIDLGVLNERLGYFIRRAQ
                     LWVFQDFIRRLSSLDISPAEFSVLVVIGANTGLSQAELALTLGIERARLVRLLHRLER
                     RGLTQRLPSSADGRRHALRLTADGQKILTRAKTLAAQHEAALKEKLGTERHQSLLESL
                     RGF"
     gene            1420..2664
                     /locus_tag="VFL49_03500"
     CDS             1420..2664
                     /locus_tag="VFL49_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911070.1"
                     /translation="MKKVSQSKAAVTRRSFSAGLGAAGIVAGVAPFNIVRAQGAALKV
                     GVLLPRSGLQASIGQDCQRGVDISAGILKNLGLPSLQIMNADTETNVDVARSRAEKLI
                     SEGAQLLIGAFDSGQTTAIAQVAEQKGIPLVINIAAAPPITEQGYKFVFRNFPTGPMI
                     LRDAFLNQKEVFQITGSAPKTAVMLHVNDTYGTSIQKGMAAMFPKFDMPYKILQEIAY
                     DPGARDLSVEIAKAKATGAEALIAVSRLNDAILLTRELVKQRWTPMAVLSIGPGWYED
                     QYLKTLGKLSDGPISLVPWYDPNKKVSKILEAALAKAYPGVNMSTNHVYTFEALLVAA
                     DAYKRAGSTDPKALADAIRTTNIKENVSPGPGIMFDAKGQNDKLKGSAIQNRGGKLVT
                     LAPKEAANAKPQWPLTPYDKRG"
     gene            2735..3607
                     /locus_tag="VFL49_03505"
     CDS             2735..3607
                     /locus_tag="VFL49_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911071.1"
                     /translation="MPIDVYLNVAVSGVLTGLVYGLMALGLSVIFGVVRVVNFAHGEM
                     MTVAMYIAVTLFSALHLDPLVMLVPIAAVLFAFGYVLQAGLINPFINRPEHSQFLLLV
                     AVAIIIVNGLLILFGPNAQNVQVAYAFDSFQFGRLIIDATKAYAALAAVVVSAALFLF
                     FRSTMLGTSIRACADNYIGALVVGLNVKRLYALTFALGAACVGAAGPMLLLIFDVTPA
                     AGPAYTLLAFVIVITGGLGSMPGALVGGVLIGLTEATAGLVFTPSAKSMFAFAILVLV
                     LLFRPQGIMGKRTA"
     gene            3604..4608
                     /locus_tag="VFL49_03510"
     CDS             3604..4608
                     /locus_tag="VFL49_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911072.1"
                     /translation="MIRRLTAGVPTRALFLLAALLVGFLVAPLFAGDYVLTVLILILY
                     FAYTGQAWNIMMGFAGQLSLGHALYVGLGAYAAAALYVHFGLGPWIGLAVAVPVAALT
                     GAFIGFLAFRFRVAGVYFAILTIAFAEFARVGFDHLGWTNASSGLFLPVAQYAHNDLW
                     HLRGHPVMFYYVLLTATVLAFVFCRALLRSRIGYFWLAIREDEEAARSAGIDTFHYKM
                     VAVVISAAMTSFAGVVYAFYYNNLFPEQVFNISRSIDIILGPIAGGVGTLFGPILGSF
                     LIEILGEGLRSLLESFGIDVPGIKQVVFGIILLLVVLAMPDGVWPWLARKLGLAERQK
                     "
     gene            4605..5333
                     /locus_tag="VFL49_03515"
     CDS             4605..5333
                     /locus_tag="VFL49_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911073.1"
                     /translation="MTALLSVNGISKRFRGLLAVDSVTFDIPDGEIFAVIGPNGAGKT
                     TLFNMIAGVLAPTAGSITFAGERIEGLSPDKICRRGLGRTFQIVRPFPALTVEDNVIV
                     GALLHRHDVDDAREHARNVLRRLDLYAKRNQTASALTLPDRKRLELARALATDPKLLL
                     LDEVMAGLRPMEVDGVVATLRELNRKSGLTILLIEHVMRAVMALAARILVLHHGAAIA
                     EGTPDAVVRNPAVIDSYLGAEAVG"
     gene            5333..6037
                     /locus_tag="VFL49_03520"
     CDS             5333..6037
                     /locus_tag="VFL49_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911074.1"
                     /translation="MLSVENLDVFYGDAQALDNIGIEIAQGAIVTIVGANGAGKSSLI
                     RTIAGMHRPARGRILYRGTDITGWPSHKVCDLGIGQVAEGRQVFPTLTVAENLAMGAL
                     VPRAQAGRERNLDRVYAMFPVLASRRHQAAGTLSGGEQQMLAIGRCLMAEPELVMFDE
                     PSLGLAPTVVQNVLTTVRDLNRAGLTCVLVEQNVAVSLKLASHAYVLENGRITLAGSG
                     EQLLRDDRVRQAYLGL"
     gene            complement(6196..6900)
                     /locus_tag="VFL49_03525"
     CDS             complement(6196..6900)
                     /locus_tag="VFL49_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7911075.1"
                     /translation="MSDSRYVISPPPQATIAVAGTDKLFPVRRIWCVGRNYIEHIREM
                     GQDERAPPFFFAKPADAIVPDGGTVPYPSLTKDMHHEVELVVALKSGGRNIPVARAND
                     CIYGYAVGIDLTRRDLQIASRDVKRPWEVGKAFDSSAPCGPLMPASKIGHPAKGKIVL
                     RVNGQVRQDGDLNQMIWNVPETISKLSEMVALEAGDVIMTGTPSGVAATVAGDKIECE
                     VEGVGKLAVTIGPPLK"
     gene            complement(7021..>7374)
                     /locus_tag="VFL49_03530"
     CDS             complement(7021..>7374)
                     /locus_tag="VFL49_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7911076.1"
                     /translation="LQLSGGQRQRVAIARALIKNAPIILLDEATAALDSESELQVREA
                     MEHLCQGRTTLAIAHRLHTVSHADRIYVVEDGRAVESGRHDELLRKGGRYASFYRLQL
                     KAQEAASPVAAVASS"
BASE COUNT         1342 a         2218 c         2237 g         1577 t
ORIGIN      
        1 ttccgtcttg gcgttaaagc attcatggaa aagaaaacgc cacaatggaa caaccgatag
       61 ttggctattt cgatcctctg accgcgtatc acccccgcgc tgcaggcgcg aatcagcagc
      121 accgcgcctt ctcgccctcg agttgatccc taccgccacc aatctcggcc tgttgttcag
      181 tccaacgaac ccagcagata ccggggctca tcgcctacgg catcaatatc cccgccaacg
      241 tccgtcgggc ggcctacttc gtcgacaaaa tgctgaaagg tgccaaggcg tccgatctgc
      301 cggtcgaact tccgaccaag gtcgaacttg tgatcaatct caaaacagcc aaggcgctcg
      361 gcacacagtg ccgccaagaa agcagcagcg ctagctgctt gagcaaaaca tcaaactgtt
      421 tttcggagag accgccgcaa aaagcggtct ttctatttgt ttgtgtgcaa tgtccgcttc
      481 tctaatcggc cggtctgggt caagcgctgc ccaccggtcc agtgtcgatg tcactcacgg
      541 gctcgtgctt ctcttcggaa tcgccgctag ggcctttcca gcatcgggtt cgagaacgag
      601 gcggaacaat ctttgggtct gccttgccgt caactgacgg caggtgcaag cggacatacg
      661 atctcgcctc tggaaagcgg acatcgctgc ggggcggggg atgatcaaat tttcagttca
      721 acaccggacg accctagaac cctcgcagcg attcaagcag cgattgatgg cgctcggtgc
      781 cgagtttttc ctttaatgca gcctcgtgtt gagccgccag cgttttggcg cgcgtcagga
      841 tcttctggcc gtccgcggtg agccgtagcg catgcctgcg gccatctgct gacgatggca
      901 gccgctgcgt caggccgcgt ctttccagac ggtgcaagag gcgcaccagt cgcgcccgtt
      961 caatgcccaa agtcagcgcg agctcggcct gggatagccc ggtattggcg ccgatgacga
     1021 ccagcacgga gaattcggcc ggactgatgt cgagcgatga tagccggcgg atgaagtcct
     1081 gaaacaccca aagctgtgcc cggcgaataa aataaccgag ccgctcattg aggaccccga
     1141 ggtcaataac gggcgcgcat gtgcgtttct gcggcgtttt gcgcagtggc acggcccgtt
     1201 tgctcacgtg cttggcgtca gacatggggt agccttgtcc tgctggtcct gacggtgttg
     1261 acgaaatttg ttgtcctagt caacaatatg gccagggttg ggacggcgaa cggctgcgcg
     1321 aacgtcgctg cggccgtgct ttctcgacct tgtgaaaact ggaaaaagtc cggcatcatc
     1381 cggaataaag gccgcgtgct cggccaggga ggacatccga tgaaaaaggt atcgcagtcg
     1441 aaggcggccg taacccgccg aagcttcagc gccggactcg gagctgccgg cattgtcgcg
     1501 ggagtcgcgc cgttcaacat cgttcgcgcg cagggtgccg cgctaaaggt cggcgtgctg
     1561 ctgccacgtt cgggcctgca ggccagcatc gggcaagact gccaacgtgg tgtcgacatt
     1621 tctgccggca ttctcaagaa tctcggtctg ccgtcacttc agatcatgaa tgccgatacc
     1681 gaaacgaacg tggatgtcgc tcgttcgcgc gctgaaaagc tgatcagtga gggcgcccag
     1741 ctgctgatcg gtgccttcga ttccggacag accaccgcga ttgcacaggt cgccgagcaa
     1801 aaggggattc ctctcgtcat taacatcgca gcggcgccgc cgatcacgga gcagggctac
     1861 aagttcgtgt tccgcaattt cccgaccggg cccatgatcc tgcgcgacgc cttcctcaat
     1921 cagaaggaag ttttccagat caccggctcg gcgccgaaaa cggcggtcat gcttcatgtc
     1981 aacgacacct acggcacgtc gatccagaag ggcatggccg ccatgttccc gaagttcgac
     2041 atgccctata aaattctgca ggaaatagcc tacgaccccg gtgcgcgcga cctttcggtg
     2101 gaaatcgcaa aggccaaggc aaccggtgct gaagcgctga ttgcggtgag ccgtctcaac
     2161 gacgcgatcc tcctcacccg tgaactcgtc aagcagcgct ggaccccgat ggcagtgctc
     2221 agcatcggtc cgggatggta cgaggatcag tatctcaaga ccctcggcaa actgtcagat
     2281 ggtccgatca gcctggttcc ctggtacgac cccaacaaga aggtcagcaa gattttggaa
     2341 gctgcgctgg cgaaggcgta tccgggcgtg aatatgtcga ccaaccatgt ttacaccttc
     2401 gaggcgttgt tggtcgcagc agacgcatac aaacgggccg ggtccaccga tccgaaggcg
     2461 ctcgccgacg ccattcgaac gaccaatatc aaagaaaatg tcagccccgg cccgggcatc
     2521 atgttcgatg ccaagggtca gaatgacaaa ctgaaaggct ccgcgatcca gaatcgcggc
     2581 ggcaagctgg tcacgttggc accaaaggaa gcagccaatg ccaaaccgca gtggccgctg
     2641 acaccatacg acaagcgcgg ctgacaagcg tcgctttgac tacgttccgg ccgccagacc
     2701 cggcggccgg atcattgctt atcaggacgc atctgtgccg atcgatgtct atctcaatgt
     2761 cgccgtcagc ggcgtgctga ccgggctggt ctacggcctg atggcgctcg gcctgtcagt
     2821 aatcttcggc gtcgtgcgcg tggtgaattt cgcccacggc gaaatgatga ccgtggcgat
     2881 gtatatcgcg gtcacgttgt tctcggcgct gcatctcgat ccgctggtca tgctggtgcc
     2941 gattgcggcc gtgttgtttg cctttggcta tgtcctgcag gccggcttga tcaacccgtt
     3001 catcaatcgg cccgagcaca gccagttcct cctgctggtc gcggttgcaa tcataatcgt
     3061 caacggtctt cttattctgt tcggccccaa cgcacagaac gtgcaggtcg cctatgcttt
     3121 tgacagtttt cagttcggca ggctgatcat cgacgccacc aaagcctatg ctgctcttgc
     3181 tgcggtggtg gtgtctgccg ctctgttcct tttcttccgc agcacaatgc tcggcacgtc
     3241 gatacgcgcc tgcgcggaca attacattgg cgctctggtt gtcggtctga acgtgaagcg
     3301 gctctacgcg ctgacattcg cgcttggtgc cgcgtgcgtc ggtgcggcgg gcccgatgct
     3361 gctgctgatc ttcgacgtca ccccggcggc aggcccggcc tatacgctgc tcgcgttcgt
     3421 catcgttatc acgggcggcc tgggttcgat gcccggcgct ctcgtgggcg gcgtcctgat
     3481 tggcctcacg gaagcaacgg caggcctcgt gttcacgccg tcagccaaga gcatgttcgc
     3541 ttttgccatc ctcgttctgg tgctgctctt tcgtccgcag ggcatcatgg gcaagaggac
     3601 ggcatgatcc ggcgactgac ggccggcgtg cctacgcgcg ccctgttcct gcttgcggcg
     3661 ctccttgtgg gtttcctcgt cgcgccattg ttcgccggcg attacgtgct caccgtgctg
     3721 atattgatcc tgtacttcgc gtacacgggc caggcctgga acatcatgat gggctttgcc
     3781 ggccagttgt cgctcggtca cgcgctctat gtcggactgg gcgcctatgc cgcggcggca
     3841 ctttacgtgc attttggtct cggtccgtgg atcggcctgg ctgttgcagt cccggtcgcg
     3901 gcgctcaccg gagctttcat cggctttctt gcctttcggt ttcgggtggc cggcgtctat
     3961 ttcgccattc tcaccatcgc atttgccgaa tttgcccggg tcgggttcga tcatctcggc
     4021 tggaccaatg cttcgtccgg tctgttcctt ccagtcgcgc aatatgccca taacgatctc
     4081 tggcacctgc gcggccatcc ggtgatgttc tactatgtgc tgttgacggc aaccgtgctg
     4141 gcttttgttt tttgccgcgc gctgctcaga agccgtatcg gatatttctg gctcgcgatc
     4201 cgtgaggatg aagaggccgc gcgttcggcg ggcatcgaca catttcatta taaaatggtc
     4261 gcggtggtga tctctgccgc aatgacgtcg ttcgccggtg tggtttacgc gttctattac
     4321 aacaatttat ttcccgaaca ggtgttcaac atttcccgct cgatcgacat cattcttgga
     4381 cccattgccg gcggggtcgg cacgcttttc ggcccaatcc ttggaagttt tttgatcgaa
     4441 atcctcgggg aagggctgcg aagcctgctg gaatcgttcg gcattgatgt tcccggcatc
     4501 aagcaggtgg tgttcggcat catcctgctg ctcgttgttt tggccatgcc ggatggcgtg
     4561 tggccctggc tcgcgcggaa gctcggcctc gcggagcggc agaaatgacg gcgcttcttt
     4621 cagtcaacgg aattagcaag cgatttcgcg gcctgctcgc agtcgacagc gtgacttttg
     4681 atattcctga cggtgagatt tttgcggtca ttgggccgaa cggcgccggc aagacgacgc
     4741 tctttaatat gatcgccggc gtgctcgcgc cgaccgccgg aagcattaca tttgccggag
     4801 agcgcatcga aggtctctcc ccggacaaga tctgcaggcg cggcctcggc cgcacatttc
     4861 agatcgtgcg gccgtttccc gctctcaccg tcgaggacaa cgtcatcgtc ggtgcgctgc
     4921 tgcatcgcca cgatgtcgat gacgcacgcg agcacgcccg taatgtgctg cgccggctcg
     4981 atctgtacgc caagcgtaat caaaccgcgt ccgcgctgac acttcccgac cgtaagaggt
     5041 tggagcttgc gcgcgcgctt gccaccgacc cgaagctgct tttgcttgat gaagtaatgg
     5101 caggcctgcg gccgatggag gtcgacgggg tcgtcgcgac tctgcgcgag ctcaaccgta
     5161 agagcggact caccatcctg ttgatcgaac atgtcatgcg ggccgtgatg gcgctggctg
     5221 cgcgtatcct cgtgcttcat catggcgcgg caatcgcgga agggacgccc gacgcggtcg
     5281 tgagaaatcc cgctgtcatc gattcttatc tcggtgcgga ggccgtcggc tgatgctcag
     5341 cgtcgaaaat ctcgatgtgt tttacggaga tgcgcaggcg ctcgacaata tcgggatcga
     5401 gatcgcgcaa ggggcaatcg tcacgattgt cggagcaaat ggcgccggca agagctcgct
     5461 catccgcacc atagcgggaa tgcacaggcc cgcgcggggc aggatcctct atcgcggcac
     5521 cgatataacc ggatggccga gccacaaagt ctgcgacctc gggatcggcc aggtcgctga
     5581 gggcaggcag gtttttccga cgctcactgt cgctgaaaat cttgcgatgg gtgctttggt
     5641 gccgcgtgca caagcgggcc gcgaacgcaa tctcgatcgc gtctacgcga tgtttccggt
     5701 cctcgcctcg cgccggcatc aggccgccgg aacgctttca ggtggcgagc agcagatgct
     5761 ggcaatcggg cgttgtctga tggcggagcc ggagcttgtg atgttcgacg agccgtcgct
     5821 tgggctggcg cccacggtcg tgcaaaacgt gctcaccacc gtacgcgatc tcaaccgggc
     5881 agggcttacc tgcgtgctgg ttgagcagaa tgtcgcggtt tcgctgaaac ttgcgagcca
     5941 cgcttatgtg ctggagaacg gccgcatcac gctcgcaggt tcgggcgagc aactgctgcg
     6001 cgatgaccgc gttcggcagg cctatctcgg cctctgagca agacttgatc tggttgaatc
     6061 ccggcgctga ttttcactcc gcccgcggaa gcgggaatcc agggccacac gataggccac
     6121 gcatttctgc tccggtcccc gcggggacaa gcggataagg cggtaacaac ttgatcggat
     6181 cacgttgtag ctgaatcact tcagcggcgg accgatcgtg acggcgagct tgccgacgcc
     6241 ctcgacctcg cactcgatct tgtcgccggc gaccgtggcc gcgacaccgg aaggcgttcc
     6301 ggtcatgatt acgtcgccgg cttcaagggc taccatttcc gacagcttcg agatcgtctc
     6361 aggcacgttc cagatcatct ggttgaggtc gccgtcctgc cgcacctgtc cgttgaccct
     6421 gaggacaatt ttgcccttcg ccgggtggcc gattttgctc gcaggcatca gcgggccgca
     6481 cggcgcggag ctgtcgaagg ctttgccgac ctcccagggg cgcttcacat cgcgcgaagc
     6541 gatctgcaga tcgcggcggg tcaggtcgat gccgaccgcg taaccgtaga tgcagtcatt
     6601 cgccctggcg accgggatgt ttcgtccgcc gcttttcagc gcgacgacca attcgacttc
     6661 gtgatgcatg tccttggtca gcgaagggta gggaacagtg cccccatcgg gaacgatcgc
     6721 gtcggcgggc tttgcaaaga aaaacggcgg agcgcgctcg tcctggccca tctcgcgtat
     6781 gtgctcgata taattgcggc cgacgcacca gatacgacga accggaaaaa gcttgtcggt
     6841 gcctgcgact gcgattgtgg cctggggcgg cggcgaaatg acgtagcggc tgtcggacat
     6901 gattgctggc cgttcgattt tcccgttgtt gtttcatgtg ccggctgcgg gatactgggt
     6961 cgccgggcaa agacgcggct gtgcgtatca ggtggggcgc gccgcagtca accgcgcgaa
     7021 ctatgacgat gcgactgcgg caacggggct ggcggcctcc tgggccttga gttgcagcct
     7081 gtagaacgag gcatagcggc cgcccttgcg gagcaactcg tcgtgccggc cggattcgac
     7141 cgcgcgcccg tcttccacga catagattct gtcggcatgc gacaccgtgt gcaaacgatg
     7201 cgcgatcgcg agcgtggtgc ggccctggca gagatgttcc atagcctcgc gcacctgcag
     7261 ctcggattcg gaatcgagcg cggcagtcgc ttcgtcgagc agaatgatcg gcgcattctt
     7321 gatgagcgcc cgcgcaatgg cgaccctttg gcgctgccca ccggacagct gcaa
//
LOCUS       DASSRL010000168         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1106827, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000168 DASSRL010000000
VERSION     DASSRL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106827"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VFL49_03535"
     CDS             complement(<1..1108)
                     /locus_tag="VFL49_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7911077.1"
                     /translation="MRANSLALRLFLSATAWTVIILLITGVVLSSLYRQAVERAFDRR
                     LGVYLRTLVADIASPEEAGEKFPQSLGEPLFELPLSGWYWQATRLDPGKNEVRSSRSL
                     WDGGLPHLQDQGIAGGADGSRAGYVGGPEDQRLRLVERNIDLGDEGHYLVAVAGDAAE
                     ISDETRSFDQALIITFSILAAVLLLTTTFQVRFGLAPLKRITDGLAAIRSGSAERLAG
                     KFPEEIAPLARETNALIETNKEIVERARTHVGNLAHALKTPLSVMVNEATAHGTEPFA
                     QKILEQADIMRDQVARHLERARLAARLTVVGSVTEAAPVVTALARTMEKIHRDKNLTI
                     DVHADDTAQFHGERPDLEEMVGNLVDNACKWASSR"
     gene            complement(1110..1778)
                     /locus_tag="VFL49_03540"
     CDS             complement(1110..1778)
                     /locus_tag="VFL49_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7911078.1"
                     /translation="MRLLVIEDDPDLNRQLATALTDAGYVVDRAFDGEEGHYLGESEP
                     YDAVILDIGLPKMDGISVLEAWRRTGRAMPVLMLTARDRWSDKVQGFDAGADDYVAKP
                     FHLEEVLARVRALLRRSAGHAKSEFNCGPVRLDTRTGRVSVNGNPVKLTSHEYRLLAY
                     LMHHTGRVVSRTELVEHLYDQDFDRDSNTIEVFVGRIRKKLGVEIIQTVRGLGYLLTP
                     PDTR"
     gene            complement(1782..>2152)
                     /locus_tag="VFL49_03545"
     CDS             complement(1782..>2152)
                     /locus_tag="VFL49_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911079.1"
                     /translation="RGVHRRAANTRFMPVFRLTTLVLLAALSTVPAQAADGPHRGACL
                     TKAEQRAAVAANKAIPLAQAIKLLREHRQYSEVVRARLCRHDQKLVYVLTLLGRSGKV
                     VTATIDAVDGEYHAGRSAEK"
BASE COUNT          435 a          645 c          666 g          406 t
ORIGIN      
        1 cacgtgatga agcccatttg cacgcgttgt cgaccaggtt tccgaccatc tcttccaggt
       61 caggccgctc gccgtgaaat tgggccgtgt cgtctgcgtg cacatctatc gtcaggttct
      121 tgtcgcggtg aatcttttcc atggtgcgcg caagcgcggt cacgacgggt gcggcctcgg
      181 tgactgatcc gacgaccgtc aaccttgcag caagtcgtgc ccgttcgagg tgccgcgcaa
      241 cctgatcacg catgatgtcg gcttgctcga ggattttctg agcaaacggt tcggtcccgt
      301 gtgcggtagc ctcgttcacc atcaccgaca gcggggtttt cagggcatgc gccagatttc
      361 cgacatgcgt tcgggcgcgc tcgacgatct ccttgtttgt ctcgatcaac gcgtttgtct
      421 cgcgtgcgag cggagcgatt tcttcaggaa attttccggc caggcgctcg gcgctgccgg
      481 aacgaatggc ggcaagtccg tcggtgatac gcttgagggg cgccaggccg aaacggacct
      541 gaaatgtcgt cgtaagcagc agtactgcgg caagaatgga aaatgtgatg ataagcgctt
      601 gatcaaacga acgtgtttca tccgaaattt cggcggcgtc gcccgccacg gcgacaaggt
      661 agtggccctc atcacccaaa tcgatattac gctcgacgag gcgcaggcgc tggtcttccg
      721 gcccgcctac gtagcccgcc cgcgagccgt cagcgccgcc ggcaatgcct tggtcctgca
      781 agtgcggcaa gccgccgtcc cacaaagagc gcgacgagcg gacttcattt ttgccggggt
      841 caaggcgcgt tgcctgccaa taccagcccg aaagcggcaa ctcgaacagc ggctcaccca
      901 aagattgcgg aaatttttcc ccagcctctt ccggcgatgc gatatcggcg acaagcgtgc
      961 ggagatagac gccgaggcgg cggtcgaaag cacgctcaac cgcctgcctg taaagcgacg
     1021 acagcacgac gccggtgatg agcaaaatga tcaccgtcca ggccgtggct gacaaaaaca
     1081 ggcgaagcgc aagcgaattg gcgcgcatgt caacgcgtgt cgggcggcgt gagcaaatat
     1141 ccgaggcctc gcaccgtctg gataatctcg acgccaagct tcttgcgaat gcggccgaca
     1201 aacacttcga tcgtgtttga gtcgcgatcg aagtcctgat cgtaaagatg ttcgaccaac
     1261 tccgtacgcg aaacaacgcg accggtatgg tgcatcagat aggccagcaa cctgtattcg
     1321 tggctggtaa gcttgaccgg gttgccgttg accgaaacac ggccggtgcg ggtgtcgagc
     1381 cgcaccggac cgcagttgaa ttcgctcttc gcgtggccgg cggaacggcg cagcagggcg
     1441 cgaacgcgcg caagcacttc ctcgagatga aatggtttgg cgacgtaatc gtcggccccg
     1501 gcgtcgaagc cctggacctt gtcgctccag cgatcacgcg cagtcagcat gagcacgggc
     1561 attgcccgac cagtccggcg ccacgcctca aggacagata tgccatccat cttcggcaat
     1621 ccgatgtcca ggatgaccgc gtcataaggt tcggattcgc cgagatagtg accctcctcg
     1681 ccatcgaacg cgcggtcgac aacataaccc gcgtcggtca gcgcggtggc aagttgtcga
     1741 ttgagatcgg gatcgtcctc gatcacgagg aggcgcacgg cttatttctc cgctgagcgg
     1801 cctgcatggt actcgccgtc gactgcgtca atagtcgcgg tgaccacttt gccactacgg
     1861 ccgagcagag tcagcacata gaccagcttc tggtcatgcc ggcacaggcg ggcgcgaaca
     1921 acctcggaat attgacggtg ttcccgcagc aattttatgg cctgagcaag gggaatcgcc
     1981 ttgttggcgg cgacagcagc gcgttgctcc gccttggtca ggcacgcgcc gcggtgaggc
     2041 ccatccgcgg cttgggccgg gacggtcgac agggctgcca gcagaaccaa ggtggtaaga
     2101 cgaaagaccg gcataaagcg ggtgttagcg gcgcgacggt gaacgccgcg tg
//
LOCUS       DASSRL010000169         5993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1526933, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000169 DASSRL010000000
VERSION     DASSRL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526933"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VFL49_03550"
     CDS             complement(<1..1066)
                     /locus_tag="VFL49_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7911080.1"
                     /translation="MPRLQLKGVNLMPAREGFDPIETASRDEITALQLERMKWTLDHA
                     YRNVAHYRKAFDKAGVHPGDLKALDDVPKFPFTVKDDLRAHYPFGMFAIPRDQVLRIH
                     ASSGTTGKPTVVGYSRRDLETWADLMARCMRAAGCKPGMLVQNSYGYGLFTGGIGFHY
                     GAERLGMTVVPVSGGMTERQVRLIADFRPDVIFVTPSYILAILDEFRTQGINPRQCSL
                     KIAMCGAEPWTNAMRAEIEEAFDLHAIDNYGLSEIIGPGVSCECIESKDGPHIWEDHF
                     YPEVIDPVTGKVLPDGEVGELVITTLTKEAMPVVRYRTRDLSRLLPGTARSMRRMEKV
                     AGRSDDMMIVRGVNVFPSQIE"
     gene            complement(1096..2523)
                     /locus_tag="VFL49_03555"
     CDS             complement(1096..2523)
                     /locus_tag="VFL49_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7911081.1"
                     /translation="MEGSRIARAKESHLDTSEILRRGGLPPQQAKAWLKALPRVTGSF
                     KRDTAAATKFWRAGDSLLAKLPRKPQRTPEQQSAAEIILCESRRLREAFLKKHADAIY
                     RRLTKNLSVFHRVDELAYDVAKLVPGLTPTSKQVDAESAFTQSEKDGIEIGQGIFLAH
                     MLALPEAGMHLCEAMLRPKQESIARVDEFIQNGVIDFGPARIERQGKAAVVTIQNPRF
                     LNAEDDDTLDDTETATDIAILDPMSEICVLRGGVVEHPKYKGRNIFSSGINLTHIYRG
                     KIPYLWYIRRDMGVVNKMFRGIATGTASPDEIYGGTREKPWVATVDGWAIGGGCQYLL
                     AMDYVIAGSDAYMTLPARKEGIIPGAANLRMPRFTGDRITRQAILMGRRLDCDTPEGR
                     TICDLVVPPGEMEAAIAQVVDDFTGSGVVSAAGNRRAIRVGEEPLDLFRRYMAVYCRE
                     QAYCHFSPALIANLEKHWNAAQRKI"
     gene            complement(2550..3326)
                     /gene="nth"
                     /locus_tag="VFL49_03560"
     CDS             complement(2550..3326)
                     /gene="nth"
                     /locus_tag="VFL49_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7911082.1"
                     /translation="MARKPARKKVPAKPRKTKAPASKHAKVKPANKRAAADAKAAQGA
                     LPQWSTAQIAEAFRRFKAANPEPKGELQHISPFTLLVAVVLSAQATDAGVNKATPALF
                     SLADTPEKMAALGEARVRDLIRTIGLFRTKAKNVVALSQKLIAEHGGQVPCSREALEA
                     LPGVGRKTANVVLNIAFGEPTIAVDTHIFRVGNRTGLATGKTPLEVEMKLEQVVPAEY
                     KRHAHHWLILHGRYTCVARKPLCEKCIIADLCLWPGKSVV"
     gene            3348..3860
                     /locus_tag="VFL49_03565"
     CDS             3348..3860
                     /locus_tag="VFL49_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HET7911083.1"
                     /translation="MIPDNADDPEPTIFSATLTPHRSLGRVGFLVLMILFGGVSFVAG
                     MLFLVIGAWPVLGFFGLDVLLLYLAFRLNYWHAKAYEEVTVTPSTLVVRKVSHVGRVR
                     EWVLNPLWVHLDKVELEEFGIDRLFLVSRGKKLAIASCLGPDEKAGFAKELGNALLEA
                     RRGPTRTVFE"
     gene            3914..4813
                     /locus_tag="VFL49_03570"
     CDS             3914..4813
                     /locus_tag="VFL49_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7911084.1"
                     /translation="MNLAKTTVTDRFTEFSPLSTAAADYDIVRRAIAHIRGNWRAQPE
                     IDAIANAAGVSATDLHHLFRRWCGLTPKAFLQALTLNHARDLLRSSASVLDTAYEVGL
                     SGPGRLHDLFVTHEAMSPGEWKAGGEGLTITYGFHPCPFGMALVMTTPRGLAGLALAD
                     AGKERAALRDMKSRWPKAKYVEDFAATAPTARRIFDAALWRKDRPLRVVLIGTDFEVR
                     VWEKLLDIPMGKLTTYSDLAARAGAPKGARAVGAAVGKNPICFVVPCHRVIGKSGELT
                     GYHWGLTRKRAMLGWEAGMSAAA"
     gene            complement(4814..5437)
                     /locus_tag="VFL49_03575"
     CDS             complement(4814..5437)
                     /locus_tag="VFL49_03575"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET7911085.1"
                     /translation="MTDRLLVLVRHGQSDWNLKNLFTGWRDVDLTEKGVVEAREAGRK
                     LKAQGIKFDVAFTSALKRAQRTLDLMLTELGQTGIPVFKNQALNERDYGDLVGLNKDD
                     ARKKWGEEQVHIWRRSYDVAPPGGESLKDTLARTLPYYVTDILPRVLRGERVLVAAHG
                     NSLRALVMVLDKHTPDSITKLNLETGVPMIYRLNADSTVASKLDLAA"
     gene            complement(5446..>5993)
                     /gene="dapB"
                     /locus_tag="VFL49_03580"
     CDS             complement(5446..>5993)
                     /gene="dapB"
                     /locus_tag="VFL49_03580"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7911086.1"
                     /translation="AKSGKVHIIGTTGLSAADEAKIKDAARSAVLVKSGNMSLGVNLL
                     AALTKRVAKTLDNSFDIEILEMHHNQKVDAPSGTALMLGRAAAEGRGIPLDKHSVRNR
                     DGHTGARKAGDIGFAALRGGTVVGEHSVIFSGAAERVELTHKAEDRMIFARGALHAAR
                     WARAQKPGLYSMMDVLGLKDF"
BASE COUNT         1102 a         1864 c         1763 g         1264 t
ORIGIN      
        1 cctcgatctg gctgggaaat acattcacgc cacggacgat catcatgtcg tcggaacgcc
       61 cggcgacctt ctccatccgg cgcattgagc gcgcggtacc gggcagcagt cgcgacaggt
      121 cgcgcgtgcg atagcgcacg accggcatcg cctccttggt gagcgtggtg atcaccagct
      181 caccgacctc gccgtcaggc aaaaccttgc cggtcaccgg atcgataacc tccggataaa
      241 aatgatcttc ccagatatgc ggtccgtcct tgctttcgat acattcgcag gaaacgccag
      301 ggccaataat ttccgacagg ccgtaattat cgatggcgtg cagatcgaat gcctcttcta
      361 tttcggcgcg catcgcattg gtccacggtt cggcgccgca cattgcaatc ttcagcgagc
      421 attgccgcgg attgatcccc tgagtgcgaa attcgtcaag aattgcaagt atatagctag
      481 gcgtgacaaa gatgacatcc gggcggaaat cggcaatcag gcgcacctgg cgctccgtca
      541 ttccccccga taccgggacg acggtcatgc caagccgctc tgcgccgtag tggaagccga
      601 tgccgccggt gaaaagccca taaccgtacg agttttgcac cagcatcccc ggcttgcagc
      661 ctgcggcgcg catgcagcgc gccatcaagt cggcccaggt ctcaagatcg cgccggctgt
      721 aacccaccac ggtgggcttg ccagtcgttc ccgaagacgc atgaatgcgc aaaacctgat
      781 cacgcggaat cgcaaacatt ccaaacggat aatgcgcgcg caggtcgtct ttgacggtga
      841 acggaaattt cggcacgtcg tcgagcgctt taagatcgcc agggtgcacg ccggctttgt
      901 cgaacgcctt cctgtaatgt gcgacattcc tgtaagcgtg atcgagcgtc catttcatgc
      961 gctccagctg aagcgccgtt atctcatcgc gggaggctgt ttcgatcggg tcgaaaccct
     1021 cgcgcgccgg catcagattg acgcctttga gctgcaaccg cggcattcaa atctccaacg
     1081 tccggcaacg caccatcaga tttttcgctg ggcggcgttc cagtgctttt ccagattggc
     1141 gatcaaagcc gggctgaaat ggcaataggc ctgctcgcgg cagtagaccg ccatgtagcg
     1201 gcggaataaa tcaagcggct cttcgccgac acgaatcgcg cgccggttgc cggcggcact
     1261 gaccacgccc gagcctgtga aatcatcgac cacctgcgca attgccgctt ccatttcgcc
     1321 tggcggcacg accaggtcgc aaatcgtgcg gccctccggc gtgtcgcaat cgagccggcg
     1381 gcccatcaga attgcctggc gtgtgatgcg atcaccggta aaccgtggca tgcgcagatt
     1441 ggccgcaccc ggaatgatgc cttccttgcg cgcaggaagc gtcatgtagg catcgctgcc
     1501 agcaatcacg taatccatcg ccagaaggta ctggcagccg ccgccgatcg cccagccgtc
     1561 gaccgtggca acccagggtt tttcgcgggt cccgccgtaa atctcgtcgg ggctcgccgt
     1621 tcccgtcgca atgccacgaa acatcttgtt caccacgccc atgtcgcggc ggatgtacca
     1681 gagatagggg atcttgccgc ggtagatgtg cgtaaggttg atcccgctcg aaaatatatt
     1741 gcggcctttg tacttgggat gttcgacgac accgccgcgc aacacgcaga tttcgctcat
     1801 tgggtcgaga atcgcgatat cggtagctgt ttccgtatcg tccagcgtgt cgtcatcctc
     1861 ggcattgagg aaacgcggat tttgtatcgt cacaacggca gccttgccct gccgctcgat
     1921 ccgggccggg ccgaaatcga tcactccgtt ttggataaat tcatcgacgc gcgcgattga
     1981 ttcttgcttc gggcgcagca tcgcttcaca cagatgcatc ccagcttcag gcagcgcgag
     2041 catgtgcgcc aggaaaatcc cctggccgat ttcgatgccg tccttctcgc tctgcgtgaa
     2101 cgcgctctcg gcgtcgacct gcttgcttgt cggcgtcaat cccggaacca gtttggccac
     2161 gtcgtatgca agttcgtcca cccgatgaaa aaccgataaa tttttcgtaa gcctgcgata
     2221 gatcgcgtcc gcatgcttct tcaggaacgc ttcgcggaga cgccggcttt cgcaaagaat
     2281 gatttcggct gccgactgtt gctccggcgt tcgctgcggt ttcctgggca atttcgccag
     2341 aagactgtca cctgcgcgcc agaattttgt cgccgccgcc gtatcgcgct tgaacgagcc
     2401 cgtcacacgc ggaagcgcct tgagccacgc cttggcttgc tgcggaggca acccgccacg
     2461 acgaagaatt tcactggtat cgagatgcga ttcttttgct ctcgcgatcc gggagccttc
     2521 catacgcgca ctctccctac tctggcactt tatacgaccg acttgcccgg ccacagacac
     2581 aaatcagcga tgatgcattt ctcgcacagc ggcttgcgtg cgacgcaagt gtagcggcca
     2641 tgcagaataa gccaatggtg ggcatgccgc ttgtattccg ccgggactac ctgctctaat
     2701 ttcatttcaa cttcgagcgg cgtcttgcct gtggccagtc cggtacggtt tccgacacgg
     2761 aagatatgcg tgtcgacagc gatggtcggc tcaccgaagg cgatattgag gacgacgttg
     2821 gcggtcttgc ggccgacccc aggtagtgcc tccagcgctt cgcgcgagca tggaacctga
     2881 ccgccatgtt cggcgatcag cttctgcgat aacgcgacaa cgtttttcgc cttggtgcga
     2941 aacaggccga tggtcctgat gaggtcgcgc acgcgtgctt cgccgagcgc cgccattttt
     3001 tccggcgtat ctgcgaggga aaaaagtgcg ggcgtcgcct tgttgacgcc ggcgtcggtt
     3061 gcctgcgccg aaagaaccac cgcgaccagc agtgtaaatg gactgatgtg ctgcagctcg
     3121 cccttcggct ccggattggc ggccttgaag cggcggaacg cttccgcgat ctgtgctgtt
     3181 gaccattgcg gcagggcgcc ttgcgccgcc ttggcatcgg cagccgcgcg cttgtttgca
     3241 ggctttacct tcgcatgctt gcttgcaggc gcctttgtct tgcgcggttt tgccgggact
     3301 tttttccggg caggttttct cgccattatt ttggtatagt gagccggatg atccccgaca
     3361 acgccgacga ccccgaaccc accatctttt cagcgacgct cacgccgcac cgctcgcttg
     3421 gacgcgttgg atttctcgtc ctgatgatcc tgttcggcgg cgtcagcttc gtggccggca
     3481 tgctgtttct cgtcattggc gcgtggccgg ttctcggctt cttcgggctc gacgtcctgc
     3541 tgctgtatct cgcctttcgg ctcaactatt ggcacgcgaa agcttatgag gaggtgacgg
     3601 ttacgccgtc aacgctcgtc gtgcgcaagg tgagccatgt cgggcgcgtt cgcgagtggg
     3661 tgctcaatcc gctctgggtc cacctcgaca aggttgaatt ggaagagttc ggtatcgatc
     3721 gacttttcct cgtctcgcgt ggcaaaaagc tggcaatcgc cagctgtctc ggaccggatg
     3781 aaaaggcggg ttttgcgaaa gaactcggca atgcgctcct ggaggcccgc cgcggtccga
     3841 cgagaaccgt gttcgagtag ccagaaatcg ggtggttgcg gcgtccggtt cgccctaaat
     3901 agcggccatg aacatgaacc tcgcgaaaac aaccgtgacc gaccgcttca ctgaattctc
     3961 gccgctttcg acggcagcgg cggactatga catcgtgcgg cgcgccattg cgcacatacg
     4021 ggggaactgg cgtgcccagc ccgagatcga tgccatcgca aacgccgcgg gcgtatcggc
     4081 gacggacctg catcatctgt tccgacgctg gtgcggactg acgccaaaag ctttccttca
     4141 ggcgctgacg ctcaaccacg cacgcgatct gctgcgctcg tcggccagcg ttcttgatac
     4201 ggcctacgaa gttgggctgt ccggcccagg caggctgcac gatctctttg ttacgcatga
     4261 agccatgtcg cccggggagt ggaaggccgg cggcgaaggc ctcaccatca cttacggatt
     4321 tcacccctgc ccgttcggca tggcgctggt gatgaccacg ccgcgcggac ttgcaggcct
     4381 cgctctcgct gacgccggca aggaacgggc ggcccttcgc gacatgaaat cgcgctggcc
     4441 gaaggcaaaa tacgtggaag atttcgctgc cactgcaccg accgcgcgcc gaattttcga
     4501 tgccgcgctc tggcgcaagg accggccatt gcgcgtggta ttgatcggca ccgatttcga
     4561 agtgcgggtg tgggagaaat tgctcgacat tcctatgggc aagctcacca cttattccga
     4621 cctagccgcc agggccggcg caccgaaagg ggcgcgcgcg gtcggcgcag cggtcggtaa
     4681 gaacccgatc tgcttcgtgg tgccgtgcca ccgcgtcatc ggcaagagcg gtgagctcac
     4741 cggttatcac tggggtctca cgcgcaagcg cgcgatgctc ggctgggaag cgggaatgtc
     4801 cgcggcggcc tgatcaggct gcgagatcaa gcttcgaagc gaccgtcgaa tcggcgttga
     4861 gccggtaaat catcggcacg ccggtctcca ggttaagctt ggtgatgctg tccggcgtgt
     4921 gcttgtcgag caccataaca agcgcccgca acgagttgcc gtgcgccgcg accagcaccc
     4981 gctcgccgcg cagcacgcgg ggcaggatgt ctgtcacgta atacggcaaa gtgcgcgcga
     5041 gcgtatcctt caggctttcg ccgccgggtg gcgccacgtc ataggaacgg cgccagatgt
     5101 gcacctgctc ctcgccccat ttcttgcgtg cgtcgtcttt gttcaacccg acaagatcgc
     5161 cgtagtcgcg ctcgttcaac gcctgatttt tgaagaccgg gattcccgtc tggcccagct
     5221 cggtcagcat caggtcgagc gtgcgctgcg cacgcttgag agcagacgta aatgccacgt
     5281 cgaacttgat tccctgcgcc ttgagcttgc gaccggcctc gcgcgcttct acgacgccct
     5341 tttcggtcag gtcgacgtcg cgccagccgg tgaacaggtt cttcagattc cagtcgcttt
     5401 ggccgtgtcg cacgagcacg agaaggcggt ccgtcatgca ggtcctcaga agtctttcaa
     5461 accaagaacg tccatcatcg aataaagtcc cggcttctgc gcgcgtgccc agcgcgccgc
     5521 gtgcagggca ccgcgcgcaa agatcatgcg atcttctgcc ttgtgcgtga gttccacgcg
     5581 ctcggcggcg ccagagaaaa tcacgctgtg ctcaccgacg acagtcccgc cacgcagggc
     5641 ggcaaagccg atgtcgcccg ctttgcgcgc cccggtgtgc ccatcgcggt ttctaacgga
     5701 atgcttgtcg agtgggatgc cgcgcccctc ggctgccgca cgaccaagca tcagcgcagt
     5761 gcccgatggc gcgtcgactt tctggttgtg atgcatctcg aggatttcga tgtcgaatga
     5821 attatcgagc gtctttgcta cacgcttcgt aagcgccgca agcaggttca caccaaggct
     5881 catgttgcct gacttgacga gcacggcact tctggcggcg tccttgattt tggcctcatc
     5941 ggctgcgcta agcccggtcg ttccgatgat gtgcaccttg ccggacttgg cag
//
LOCUS       DASSRL010000170         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1719325, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000170 DASSRL010000000
VERSION     DASSRL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719325"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1191
                     /locus_tag="VFL49_03585"
     CDS             40..1191
                     /locus_tag="VFL49_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911087.1"
                     /translation="MRKTLLFAAATLLAVTSANAQQTVKIGLIMSYSGQFADAAAQMD
                     NAIKLYVKQHGDTVAGKKIEIIRKDTGGPNPDVAKRLAQELVVRDKVDVLAGFVLTPE
                     ALGAGDVSAEAKKLMVVMNAATSIITTKSPYIARTSLTIPQLENAFGQWAYKFGIRKV
                     YTMVSDYGPGIDAETWFTKGFKDAGGEIVGSVRMPIASADFSSFVQRAKDIGPEAIFI
                     FIPGGTQPASMTKAMQERGFPAGKILGQGEITMDDNALKSMGDAAVGIRTVFHYGWEH
                     QSPANEAFVKAYRAEFKRNPDVFSVGGWDGMQLIYEALKKTGGKADGDSLIAAVKGTA
                     WESPRGPISIDPNTRDIVQNVYIRSVKKVDGASQNVEFDKIENVKDPAK"
     gene            1289..>2108
                     /locus_tag="VFL49_03590"
     CDS             1289..>2108
                     /locus_tag="VFL49_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7911088.1"
                     /translation="MNALNLPRPEWMTVDLVLLEEQARRFIADEFVPHLDRWHEAAIY
                     DRDVWTKAGEAGLLCASMPEEYGGAGGTFAHEAVINRELALAGFDTFGAPLHSGIVAP
                     YILHYGTEEQKKRWLPKLATGAFVGAIAMTEPGTGSDLQGVRASARKSGNGYVLNGSK
                     TFITNGQHANLIIVVAKTDPKAGAKGTSLFVVETDDAQGFGRGRKLKKLGMDSADTSE
                     LFFEDVQLPASSLLGTEEGQGFYQLMKELPQERLIVAVHAVAMMERALALTVDYV"
BASE COUNT          436 a          623 c          671 g          378 t
ORIGIN      
        1 gagccgtcgc gtttaaaaac ggcactcggg agaacttaca tgcgaaagac tttgcttttt
       61 gcggcggcga cactgctggc ggtcacttca gcgaacgcgc agcagaccgt caagatcggg
      121 ctgatcatgt cctattccgg ccagttcgct gacgccgcgg cccagatgga caatgccatc
      181 aaactttacg tcaagcagca cggcgacacc gttgccggca agaagatcga gatcatccgc
      241 aaggacaccg gtggtccgaa tccggacgtc gccaagcgcc tggcgcagga acttgtcgtg
      301 cgtgacaagg tcgacgttct cgctggcttc gtgctgacgc cggaagccct gggggcgggt
      361 gacgtttcgg cggaagcaaa gaaactcatg gtggtcatga atgcggcaac ttcgatcatc
      421 accaccaagt ccccgtatat cgctcgcacc tcgctcacca ttccgcagct tgaaaacgca
      481 ttcgggcagt gggcttacaa gttcggcatc cgtaaggtct acaccatggt gtccgattac
      541 gggccgggga tcgatgcgga gacctggttc acaaaaggtt tcaaggatgc gggcggcgag
      601 atcgtcggct cggtacgcat gccgatcgcc agcgctgatt tctcgtcatt cgtgcaacgc
      661 gcgaaggaca tcggtcccga ggccatcttc attttcatcc cgggcgggac gcagccggcg
      721 tcaatgacca aagcgatgca ggagcgcggc ttccccgcgg gcaagattct cggccagggc
      781 gaaatcacga tggatgacaa cgcgctcaag agcatgggcg acgccgccgt cgggatccgg
      841 accgtttttc actacggatg ggagcaccag tcgccggcga acgaggcctt cgtgaaggcc
      901 taccgggccg aattcaagcg caatcccgac gtgttttcgg tcggaggctg ggacggcatg
      961 cagttgatct acgaggcgct gaagaaaacc ggcggcaagg ccgacggaga ttccctgatc
     1021 gccgccgtca agggtaccgc atgggaaagt ccgcgcgggc ccatttcgat cgatccgaac
     1081 acccgcgata tcgttcagaa cgtgtacatc cgcagcgtga aaaaagtcga cggcgccagc
     1141 cagaacgttg agttcgacaa gatcgaaaac gtcaaggatc cggcaaagta gatgtgaaat
     1201 atgctccgag ggttggccgc cgcccggcgg ctgacccctc gcgcgcgtgc gccggcggcc
     1261 ttattgtgga gcaaaggcga gtcgtccgat gaatgccctc aacctgccgc ggccggagtg
     1321 gatgaccgta gatctcgtgc tgctcgagga gcaggcgcgg cgcttcatcg ccgacgaatt
     1381 cgtgccgcat ctcgatcgct ggcacgaggc cgccatctat gatcgcgacg tctggaccaa
     1441 agcgggcgaa gccgggctgc tttgcgcttc aatgccggaa gaatacggcg gcgccggcgg
     1501 aaccttcgca cacgaggcgg tgatcaaccg cgagttggcg ctcgcgggtt tcgacacatt
     1561 cggtgcgccg ctgcactccg gcatcgttgc gccttacatt ttgcattacg gcaccgaaga
     1621 gcagaagaag cggtggttgc cgaagcttgc caccggcgcc ttcgtcggtg cgatcgcgat
     1681 gacggagccg ggcaccggct ccgatctgca gggcgtgcgt gcatcggcca ggaagtccgg
     1741 caatggttac gtcctcaacg gctcgaagac attcatcacc aacggtcagc acgccaatct
     1801 catcatcgtt gttgcgaaaa ccgatccgaa agccggggcg aaaggcacct cgcttttcgt
     1861 ggtcgagacc gacgatgcgc aggggttcgg ccgcggcagg aagctgaaga agctcggcat
     1921 ggattcggcc gacacctcgg agctgttctt cgaggacgtg cagctgccgg cttccagcct
     1981 gctcgggacg gaggaaggac agggtttcta tcagctgatg aaggagctgc cgcaagagcg
     2041 gttgatcgtc gccgtccatg cggtcgccat gatggagcgg gcgctcgcac tcaccgtcga
     2101 ctacgtca
//
LOCUS       DASSRL010000171         6713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_774052, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000171 DASSRL010000000
VERSION     DASSRL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774052"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFL49_03595"
     CDS             complement(<1..266)
                     /locus_tag="VFL49_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911089.1"
                     /translation="MAKQRISYVPLEKMTPKMRQEMERCQREGTPRPESSAIRAHVEA
                     CFWFFADSWNNIFRNGVLDHSIKELCRLYVSRSVQCEYCGNQRSL"
     gene            complement(290..1834)
                     /locus_tag="VFL49_03600"
     CDS             complement(290..1834)
                     /locus_tag="VFL49_03600"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET7911090.1"
                     /translation="MLRTGEHYLKSLNDGRTVWVGNTKIDNVATHPLTRDYALRTAEF
                     FDLHRRPELQDQLTFVDKNGERRSMMWFLHRSKEELVRKRRYHEFIMRHFVAASMPRT
                     PDSQNYMLVTYIDDPEPWEKASIGAEGRGLADNIRKFWQYAMDHDLVVAPHFVDPQAD
                     RSDPNAHANSPALRIVSANDEGIVVNGVKAIGTASAFGDFLHLGVFFRPGAKGDQIIY
                     GVCPANLKGITIVCRESVVAADPVEHPLASQGDELDATVMFDNVLIPWKYVFHIGNPD
                     HAKLYPQRVFDWGHYYALFRQAVRAELLAGLAILMCEHLGTAKIEAVQARLARIIGFQ
                     QSTYAHVLAAEDQGFFTPGGLYKPDIQLFNWGRVYFLQNLGPMVDELIDLCGRSAIMF
                     PTEQQWQNAAMRPWFEKLNKGATGEAYDRVKIARVIRDLYLTDWGSRLFMFENFNGTP
                     LQTLLSLTMKRAEFTGSGPFAEFARRVVGIEAKCDAVTEYKTVADYAKAQDSARGSKP
                     AIQAAE"
     gene            complement(1863..2399)
                     /locus_tag="VFL49_03605"
     CDS             complement(1863..2399)
                     /locus_tag="VFL49_03605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7911091.1"
                     /translation="MLPTAPQDRGFDRSTHCVPSTEFRNALSKFATAVSVITTAGPAG
                     AAGVTCSAVCALSDDPAMVLVCVHGKSATNAAIRQNRILCVNCLQAGQSELSQAFAGI
                     GAIPMHKRFALGTWDALVTGAPRCKDALIALDCEVAEVRDIGTHSVFVAKVLATSEAG
                     QGEPLLYQRRAYVTTCAL"
     gene            2492..3445
                     /locus_tag="VFL49_03610"
     CDS             2492..3445
                     /locus_tag="VFL49_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7911092.1"
                     /translation="MDINYIPNSYSVELDLQHLRVLDVLLRERSLTRAAKVLDVTQPA
                     LSKTLARLRRYFDDPLFVRVSHRMEPTPKALALTQPVGDVLEKMRALRGEHVVFDPRV
                     STRTFQFCVVDAGVIKLLPPLLNRVMVEAPNVRLRVVQLDGEHLESWLETGTIDFAMG
                     SFPALVKGIRRQFLWVEEYVSVVNRGHPRVSSNPSLREFAAENHVLVSTLGTGHAHKL
                     AERAVEAAIPPQNIIYRVPIFIAAAVIAKHTNAIATLPMSIATVLAEDLNLEIVRPPI
                     KLPKIEIYQYWHNRFHREPGNKWIRSVFSSLFRVPGTHPRN"
     gene            3468..5138
                     /locus_tag="VFL49_03615"
     CDS             3468..5138
                     /locus_tag="VFL49_03615"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7911093.1"
                     /translation="MSDSEFNAIPVAVVGGGPVGLMLALFLDWHGVRSVLFNTEATTR
                     WHPKGSTEGSRTMEHFRRLGIAGEIRKLGLPEDHPTDVAYFTRFGAHELARLHMPSAD
                     EARQQVAASAKTDQIPEPIHRANQMHVERFLFEHARTRPNITMHFGWNVEQFVQDEEQ
                     VRLAAVNSEGIKETWHARYLVGCDGGRSGVRRALGIRFRGESGLEQQYFGGRMFSTYV
                     RVPALYRKFLGARRAWQYWAVNPEIRSSLIAVDGKDEFLFRARASEPDTPPNDIVVAD
                     AMRRCVGADVDVKIIAHEPWTAGMALVAESYADRRVLLAGDAVHLFTPTGGFGMNTGI
                     DDASNLAWKLAAMLQGWGGSGLLSSYEAERMPVALRNTDAARRLTANIGETDIDPAIE
                     FRTPAGEAARLIAGKMFAGFGEQFASIGVQLGARYDGSQVIDDGGDPPADSLTEYVPT
                     SLPGGRAPHVWLDAGRGEGSSLFDRLGRGFTLLRLGPRAPDVSGIVEAAVARQLPLDV
                     LDIADSDTRDLYGCDLALIRPDQYVAWRGNDTMQDAAALLARVCGHRT"
     gene            5215..6258
                     /locus_tag="VFL49_03620"
     CDS             5215..6258
                     /locus_tag="VFL49_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7911094.1"
                     /translation="MPTPSKSEKIPVTVLTGYLGAGKTTLLNRILSEPHGKKFAVIVN
                     EFGEIGIDNELVVNADEEVFEMNNGCICCTVRGDLVRIIDGLMRRKGKFDAIIVETTG
                     LADPAPVAQTFFMDDAVGSRTKLDAVVTVADAKWLTDRLKDAPEAKNQIAFADVILIN
                     KTDLVAPEDLSELEARIRGLNPYARLHRTERAKIDIGEVLGRNAFDLDRILEIEPSFL
                     EADDHHHDHDHHDEHDHDHHHHGNGLKHYHDEEMQSVSLKTDQPLNPDKFFPWIQDLV
                     AKDGPSILRCKGILSFKDDPERFVFQGVHMILDGDHQRPWRADEKRDSRVVFIGRNLP
                     EDRIRQGFESCIA"
     gene            6311..>6713
                     /locus_tag="VFL49_03625"
     CDS             6311..>6713
                     /locus_tag="VFL49_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET7911095.1"
                     /translation="MNADAPIVGVHFLGPVPVFVLGDEALLFAGQGEEKRVVVHAGAI
                     LHSISDGKRIVSGGDDGKVIETGPDGELRELASDEKKRWIDQVALGPDGSVAWSAGKT
                     AHVRTRKDELRAFDAPSTVAGLAFAPKGFRLA"
BASE COUNT         1272 a         2035 c         2088 g         1318 t
ORIGIN      
        1 agcgatcgct ggttgccgca atactcgcat tgcacggaac gcgaaacgta aagccggcaa
       61 agctccttga tcgaatggtc aaggacgccg tttcggaaaa tgttgttcca ggagtcggcg
      121 aagaaccaga agcaggcttc cacgtgcgcg cggatggctg aactttccgg acgcggcgtg
      181 ccttcgcgct ggcagcgctc catctcctgc cgcatcttcg gcgtcatctt ttcaaggggt
      241 acgtagctga tgcgttgttt tgccattgtc gttcctttca atgcctgggt tattcggccg
      301 cctggatggc gggcttcgat ccgcgtgcgg agtcctgcgc cttggcatag tccgcgacgg
      361 tcttgtattc ggtgacagcg tcacatttcg cttcgattcc gacaacgcgt cgcgcaaatt
      421 cggcaaacgg gccggaaccg gtgaattcgg cgcgcttcat ggtgagcgaa agcagcgtct
      481 gcagcggcgt tccattgaaa ttctcgaaca tgaacagccg gctgccccag tcggtgagat
      541 aaaggtcacg aatgacgcgg gcgatcttca cccggtcata ggcttcgccc gtcgcgcctt
      601 tgttgagttt ctcgaaccag ggccgcatgg ccgcgttctg ccactgttgc tcggtcggga
      661 acatgatcgc gctgcggccg cacaggtcga tcagctcatc caccatcggt ccgaggttct
      721 gcaggaaata gacgcggccc cagttgaaga gctggatatc gggcttgtag aggccgcccg
      781 gcgtgaagaa gccctgatct tccgccgcga gcacatgcgc ataggtcgac tgctggaagc
      841 cgatgatgcg tgcgaggcgg gcttgcaccg cctcgatctt cgcggtgccg agatgctcgc
      901 acatgaggat tgcgaggccg gccagcaatt cggcgcgcac ggcctgacgg aacagcgcgt
      961 aataatgtcc ccagtcgaag acgcgctgcg ggtaaagctt ggcatggtcg ggattgccga
     1021 tgtggaacac gtatttccac gggatcagca cattgtcgaa cattacggtg gcatcgagct
     1081 cgtcaccctg cgacgcaagc gggtgctcga ccgggtcggc cgcaacgacg ctctcgcggc
     1141 agacaatggt gatgcccttc agatttgccg ggcatacacc gtagatgatc tgatcgccct
     1201 tggcgcctgg gcggaagaac acgccaaggt gcaggaagtc gccgaaggcc gaagcggtgc
     1261 cgatggcctt gacgccgttg accacgatgc cttcgtcatt ggccgatacg atgcgcagcg
     1321 ccggcgaatt tgcgtgtgca ttcgggtccg atcggtcagc ctgcggatcg acgaagtgcg
     1381 gcgccacgac caggtcgtga tccatcgcgt attgccagaa tttgcggata ttatcggcga
     1441 ggccgcggcc ttcagcgccg atcgatgcct tctcccacgg ctcgggatcg tcgatgtagg
     1501 tgaccagcat gtagttctgc gagtcaggcg tgcgcggcat cgacgccgcc acgaagtgcc
     1561 gcatgatgaa ttcgtggtag cggcgcttgc gaaccagctc ttccttgctg cgatgcagga
     1621 accacatcat tgaacgacgt tcgccgttct tgtccacgaa cgtaagctgg tcctgcagtt
     1681 ccggacgccg atgcaggtcg aaaaattccg cggtgcgcag cgcatagtcg cgcgtgagcg
     1741 ggtgcgttgc gacgttgtcg attttggtgt tgccgaccca gacggtgcgc ccgtcgttca
     1801 gcgacttcag gtagtgctcg cccgttcgca gcatgacgat ctcccgatct gggtccggct
     1861 cgttacaagg cgcacgtcgt cacataggcg cgccgctggt agagcagggg ctcgccttgt
     1921 ccagcttccg acgttgccag aacttttgcg acaaacacgc tgtgcgtccc gatgtcgcgc
     1981 acttcggcga cttcgcagtc gagcgcaatc agcgcgtcct tgcagcgcgg cgcaccggtc
     2041 accaacgcgt cccacgtgcc cagcgcgaag cgcttatgca tcgggatcgc gccaatcccg
     2101 gcgaatgcct gcgatagctc gctttggcct gcttggaggc aattcacgca aaggattcta
     2161 ttttgtctga tggcggcatt ggtcgcgctc ttgccgtgca cgcagacaag caccatcgca
     2221 gggtcgtcac tcaacgcgca gacggccgag caggtcactc cggccgcgcc tgcggggccg
     2281 gcggtcgtga taacggatac ggccgtggcg aacttcgaaa gcgcgttgcg aaattcggtc
     2341 gaagggacgc agtgcgtcga tcggtcgaac ccacgatcct gcggcgcggt cggcagcatg
     2401 gcagcgtttc ccctgcgggc aggtccggcg cgccggccca cccatcgttg ccgctagcct
     2461 agggcgatgc ccgcgcgtat actatcaatc gatggatata aattacattc caaatagtta
     2521 ttcggtcgag ctcgatctcc agcatttgcg cgtgttggac gtgcttttac gtgagcgcag
     2581 cctgacgcga gccgccaagg tccttgatgt aacccagccc gcgctgagca agacgctcgc
     2641 gcgcctgcgc cgctatttcg atgatccgct tttcgtgcgg gtctcgcacc ggatggagcc
     2701 gacgccgaag gcgctcgcgc tgactcagcc ggttggcgac gtgcttgaaa aaatgcgcgc
     2761 gctgcgcggc gagcacgtgg tgtttgatcc gcgcgtctcc acacggacat tccagttctg
     2821 tgtcgttgat gccggcgtca tcaagctgct gccgccattg ctcaatcgcg tcatggtcga
     2881 ggcgccgaac gtgcgtctgc gcgtggttca gctcgacggc gagcatctcg aatcgtggct
     2941 ggagaccggc acgatcgatt ttgcgatggg ctcattcccg gctctggtga aaggaatccg
     3001 ccgccaattc ttatgggtgg aggaatacgt cagcgtggtc aatcgaggcc atccgcgggt
     3061 atcgagcaat ccgtcgctgc gcgagttcgc tgccgagaac cacgtgctgg tgtcgacact
     3121 cggcaccggc cacgcgcaca agctcgcgga gcgcgcggtc gaggcagcca tcccgccaca
     3181 gaatattatc taccgggtcc cgatctttat cgcagcggcg gtgatcgcca agcataccaa
     3241 tgcgatcgcg acgctgccga tgtcgattgc gaccgtgctg gccgaggatc tcaacctcga
     3301 gatcgtcagg ccgccgatca agctgccgaa gatcgaaatc taccagtact ggcacaaccg
     3361 gtttcaccgc gaacccggca acaaatggat tcgttcggta ttctccagcc tgttccgtgt
     3421 gccgggaacg cacccccgca attgacgcaa cacgcgtcgt tcgcagcatg tctgattccg
     3481 agttcaatgc cattcctgtt gccgtcgtcg ggggcgggcc ggtcggattg atgctggctc
     3541 tgttcctgga ttggcatggc gtacggtcgg tgctgttcaa cacggaagcg accacacgct
     3601 ggcatccaaa gggcagcacc gaagggtcgc gcacgatgga gcattttcgc cggctcggca
     3661 ttgccggcga aatccgcaag ctcgggctgc ccgaggatca tccgacagac gtcgcctatt
     3721 tcacccgttt tggtgcgcat gaacttgctc gtctgcacat gccatcggca gacgaagcca
     3781 ggcagcaggt cgcagcgtcg gcaaaaaccg accagattcc cgagccgatt catcgcgcca
     3841 accagatgca cgtcgagcgg ttcctgtttg agcatgccag gacgcgtcca aacatcacga
     3901 tgcatttcgg ctggaacgtc gagcagtttg tccaggacga ggagcaagtt cggctggcgg
     3961 cggtgaactc ggaaggcata aaggagacct ggcacgcccg gtatcttgtc ggttgcgacg
     4021 gtgggcgcag cggcgtcagg cgtgcgctcg gcatcagatt tcgcggtgag tccgggctgg
     4081 aacaacagta tttcggcggg cgaatgtttt cgacctacgt gcgcgtgccg gcgctttacc
     4141 gaaaatttct cggcgcacga cgcgcctggc agtactgggc ggtcaatccc gaaatcaggt
     4201 cgagcctgat tgctgtcgac ggaaaagacg agtttctgtt tcgtgccagg gcgagcgaac
     4261 ccgacacgcc accgaatgac attgtcgtcg ccgatgccat gcgccgctgc gtcggtgccg
     4321 acgtcgatgt gaaaatcatc gcacatgagc cgtggacagc cggcatggcg ctggtggcag
     4381 agagctacgc cgaccgtcgt gtgctgctcg caggtgatgc cgtgcacctg ttcacgccga
     4441 ccggcggttt cggcatgaac accggcatcg acgatgcatc caacctcgca tggaagcttg
     4501 cggccatgct gcaaggctgg ggtggcagcg gcctcctgtc atcgtatgaa gcggagcgca
     4561 tgccggtcgc gttgcgaaat acagacgcgg cccgccggct gaccgccaat atcggtgaaa
     4621 ccgacataga ccccgcaatc gagttccgca cgcctgcggg cgaggcagcg cgcctgatcg
     4681 ccggaaagat gtttgccggg ttcggcgagc agttcgcgtc gatcggggtg caactcggcg
     4741 cccgatatga cggatcgcaa gtgatcgacg atggcggtga tccgcctgcc gacagtctga
     4801 cagaatacgt tccgaccagt ttgcctggcg gccgcgcgcc gcatgtctgg cttgatgcgg
     4861 gccggggcga gggcagttcg ctattcgacc ggctcggccg cggtttcact ttgctgcggc
     4921 ttgggccgcg ggctcccgac gtttccggga tcgtggaagc ggcggtggcc cgccagttgc
     4981 cgctcgacgt gctcgatatc gccgacagtg acacccgcga cctctatggc tgcgatctcg
     5041 cgctgatacg acccgatcaa tatgtcgcct ggcgggggaa cgacacgatg caagacgccg
     5101 cggctcttct ggcacgggtt tgtggacacc ggacgtaggc gcgccctgcc gtgacacctt
     5161 ttgtggtccg gcatgaaatt gcctatatga ccgtcaaacg gagagcccgc ttcaatgcca
     5221 acccccagta agtccgaaaa aatcccggtt accgtgctta ccggctacct cggcgccggc
     5281 aagaccacgc tgctcaaccg gatcctgtcc gaaccgcacg gaaagaaatt cgccgtcatc
     5341 gtcaacgagt tcggagagat cggcatcgac aacgagctgg tggtcaacgc cgacgaggaa
     5401 gtgttcgaga tgaacaacgg gtgcatctgc tgcaccgtgc gcggtgacct cgtgcggatc
     5461 atcgacggcc tgatgcgccg caagggcaaa ttcgacgcga tcatcgtgga aacgacgggc
     5521 ttggcggatc ctgcgccggt cgcgcagaca ttcttcatgg acgatgctgt cggctcacgt
     5581 accaaacttg atgcggtcgt aacggtcgct gacgcgaaat ggctgacgga tcgcctcaag
     5641 gacgcgccgg aagcaaagaa ccaaatcgcc ttcgccgacg tcatcctcat caacaagacc
     5701 gatctcgtgg cgcccgaaga tctgagtgag ctcgaggcgc gaatccgcgg actcaatcct
     5761 tacgcgcggc tgcatcgtac agagcgcgcc aagatcgaca tcggcgaggt ccttggccgc
     5821 aatgccttcg atcttgatcg cattctcgag atcgagcctt cgttcctcga ggccgacgat
     5881 catcaccacg accacgatca tcacgacgaa cacgaccatg atcatcacca ccacggcaat
     5941 ggcctgaagc attaccacga tgaggagatg cagtcggttt cgctcaagac cgaccagccg
     6001 ctcaatccgg acaagttctt tccgtggatt caggatctcg tcgcaaagga cggcccgagc
     6061 attttacggt gcaagggaat cctgtcattc aaggatgatc cggagcgttt cgtattccag
     6121 ggcgtgcaca tgatcctcga cggcgatcac cagcggccgt ggcgcgcgga tgaaaagcgc
     6181 gacagccggg tcgtgttcat cgggcgcaat cttccggaag accggattcg ccagggcttc
     6241 gaaagctgca tcgcctgacg tgagcgactc ccagatcccg cttccttccc ttgccgaccg
     6301 cgtccgttcc gtgaacgccg atgccccgat cgtcggcgtg catttcctcg gacctgtgcc
     6361 ggtgtttgtc cttggcgacg aggccttgct tttcgccggt cagggtgaag aaaagcgtgt
     6421 cgtcgtccac gctggcgcca ttctccatag tatttccgac ggaaagcgca tcgtttccgg
     6481 cggcgacgac ggcaaagtga tcgaaacggg cccggacggc gaacttcgcg agcttgcgtc
     6541 ggacgaaaag aaacgatgga tcgatcaggt cgcgctcggt ccagacggct ctgttgcctg
     6601 gtcggccggc aaaaccgcgc acgtgcgcac acgcaaagac gaactgcgcg cgtttgacgc
     6661 gccgtcgacg gttgccgggc ttgcctttgc accgaaagga ttccgtcttg cag
//
LOCUS       DASSRL010000172         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1159682, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000172 DASSRL010000000
VERSION     DASSRL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159682"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1365
                     /locus_tag="VFL49_03630"
     CDS             349..1365
                     /locus_tag="VFL49_03630"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7911096.1"
                     /translation="MKSGISLRSAVPKTVMPLGRLAQVTDPKVPTQLELWSNASLAPA
                     RESVARGRVDFGAELAEAKFSELDLTVSDETIWCYMRPNGPPSFTPTLLHELISMRRL
                     LQRLFAGLRPGEAAPVKYFVGGSRIPGVYNLGGDLNFFIKSIRARDVEALRSYAHDCV
                     DVAYHMMMGFHLPVITIALVQGDALGGGFEGALSFNVLVAEKRAKFGLPEILFNLFPG
                     MGAFSFLSRKLDVTRAHKMILSGRVFSAPELYELGLIDILAEDGRGEEAVQEYISHNR
                     RHHTVHRRVREVGTRVNPLSLDELRDVTDIWAEHAMKLQETDLRNMERLAGAQLRRVQ
                     RVNT"
     gene            complement(1381..3954)
                     /locus_tag="VFL49_03635"
     CDS             complement(1381..3954)
                     /locus_tag="VFL49_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7911097.1"
                     /translation="MNTGFFRKAFVPGMPGFRVRALLSWVASRLRNRPDSEHELTVNR
                     LALSGTAFMYLIVAAMFGRTDAAQMLREQWLYFALYEIVSISLFVHLLCRPGISVGRR
                     LIGMISDLGLFSYGMYVGGEAFAPLYPIYLWTIFGNGFRFGVPYLFAATATSVVGFGI
                     AIVSAPFWHEHRGLAAGLMAGLILLPLYVSTLIRKLSQAKRQAEEANRAKGAFLASIS
                     HEFRTPLNAIIGLSDLLGKTRLDSEQEEMSETIGKSGRQLLALINSILDFSRAESGRI
                     AVKAEDFDLVSMLSEIRSMLALEAQRKNVGLTFHCTARTPQLLVGDKNHLEEVLTNLA
                     GNAVKFTEHGYVTIAVDAVARDNGRVRLRFEVTDTGIGISPEAQSRIFDRFTQADETI
                     IDRFGGTGLGLAIAKQLVELQGGRIGVESAPEKGSTFWFEIDFKPQAQDYPHFYLPPT
                     PVVVVGGGENLHSVVSSCVPIAKVVADMDEAAVALAALRADGVRRPIAIVQRQACANA
                     DEPTFRRIAGDNLGCAPALVLVTEERTEGLPPPVTRNRFVTTLTEPVNSTELAAALRI
                     ARGNMSGERHEQSRSLEIAPAGRSLSILVAEDKRTNQIVIAKILDRAGHRVTLVDNGE
                     AALDILEMQEFDLVLMDVNMPVMNGIEATKLYRFAALGRPHVPVVALTADATEEVARR
                     CEEAGMDACITKPIEPNRLLEIIATLVPDEGKPAQETPSSAEITSHPAARPRLRTASP
                     SAVDAQTLDALEQLGGKEFVDELASQFIQDGGEILDVLAEAAAAGDLKAFREQLHALR
                     SAAANIGARGIYEMCLGWRHIAPEDFAVRGEAHLKKLNEEFERVRMGLRGRLSGHTEA
                     A"
     gene            complement(3963..>4364)
                     /locus_tag="VFL49_03640"
     CDS             complement(3963..>4364)
                     /locus_tag="VFL49_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7911098.1"
                     /translation="TESIVMHNADAVSKDLQQLRELGVGISIDDFGTRYSTLAYVKHF
                     PVDRLKIDQCFVRDITTNPSDAAIVRAIVSLGHSLDLEIVAEGVETVQQTTMLRAEGC
                     DEVQGFYFGEPMSAEDLIAFVQENRARARSA"
BASE COUNT          824 a         1333 c         1384 g          823 t
ORIGIN      
        1 caataggatc ggcgctgccc gaggaatcgc acgccgccgc ccgtcgcgat cccgcccccg
       61 tcaaggttca tcggccagca gaaataatgg gtgaggcaaa accgcgcaat gtccggtcgt
      121 cgacttgaat tttatgtcgg aaagacggcc tccagggtgc caaacactga aacagaaggc
      181 gattcccgtg tggcttttgg cgctttagga cctgactgca atcgcagctt ttgtgacggc
      241 catcacagtc acgattaacc atcccacgga acaaccatac cgcgaaggtt aataagtgat
      301 taaagttcct ggcggggcaa agtgctgtct ggatgggaca gaccggtaat gaaaagtgga
      361 attagcttgc gttcggctgt gccaaagacc gtcatgcctc tgggacgtct ggctcaggtc
      421 acggatccca aggttcccac gcagctggag ctttggtcca acgccagcct tgcacctgca
      481 cgcgagtcgg ttgcgcgagg ccgcgtcgat ttcggcgccg aactcgctga agcgaagttc
      541 agcgaactcg atctgaccgt cagcgacgag accatctggt gctatatgcg gccaaacggg
      601 ccgccgagct tcacgccgac gttattgcac gaactgatct cgatgcggcg cctgctgcag
      661 cgtctgttcg ccggcctgcg cccgggcgag gccgcgccgg tgaagtattt tgtcggcggt
      721 tcgcgcatcc cgggcgtgta caacctcggc ggcgatctga attttttcat caagagcatc
      781 cgcgcccgtg atgtcgaggc gctgcgcagc tacgcgcatg actgcgtcga cgtcgcctat
      841 cacatgatga tgggattcca tctgccggtg atcacgatcg cgcttgtgca aggcgacgcg
      901 ctgggcggcg ggttcgaagg cgcattgtcg ttcaacgtcc tggtcgcgga aaagcgggcc
      961 aagttcggtt tgccggaaat cctgttcaac ctgtttccgg gaatgggcgc gttcagcttt
     1021 ctgtcgcgca agctcgacgt aacgcgtgcg cacaagatga tcctcagcgg tcgcgtgttc
     1081 agcgcgccgg agctctacga gctggggttg atcgatatcc tggccgagga cgggcgcggg
     1141 gaagaggctg tgcaggaata catttcgcat aatcgccggc accataccgt gcaccgtcgc
     1201 gtgcgcgagg tcggcacgcg ggtaaacccg ctgtcgctcg atgaattgcg cgatgtcacc
     1261 gacatttggg ccgagcacgc gatgaagctc caggagacgg acttgcgcaa catggagcgg
     1321 cttgccggcg cccagctgcg gcgggtgcag cgcgtcaata cgtaaacctg ccgccgttcg
     1381 tcaggcagct tcggtgtgcc ccgaaagccg gccgcgcagc cccatgcgca cgcgctcgaa
     1441 ttcctcgttc agtttcttga ggtgggcttc gccgcggacc gcgaaatcct cgggcgcgat
     1501 gtgccgccag ccgaggcaca tctcgtagat tccgcgcgcg ccaatgttgg ccgccgcgct
     1561 gcgcaaggcg tgcagctgct cgcggaaagc cttgagatcg cccgccgcag cggcttcggc
     1621 gaggacgtcg aggatctcgc cgccgtcctg gatgaactgg gacgccagct cgtcgacgaa
     1681 ttccttgcca ccaagctgct cgagcgcgtc gagcgtctgc gcatcgacag ccgaaggaga
     1741 agcggtgcgc agacgcggcc gcgccgccgg atggcttgtg atctcggcac tgctcggcgt
     1801 ttcctgcgcg ggcttgcctt cgtccggaac gagcgttgcg atgatctcga gcagccgatt
     1861 gggctcgatc ggcttggtga tgcaggcgtc catgcccgcc tcctcgcagc ggcgcgcaac
     1921 ttcttcggtt gcgtcggccg tcagcgcgac gaccggcacg tgcggcctgc ccagtgccgc
     1981 aaaacgatag agcttggtcg cctcgatgcc gttcatgacc ggcatgttga cgtccatcaa
     2041 aaccaggtcg aattcctgca tctcgagaat gtcgagcgcc gcctcgccgt tatcaacgag
     2101 cgtaactcga tgtccggcgc gatcgagaat cttcgcgatc acgatctgat tggtgcgctt
     2161 gtcctccgct accaggatcg agagcgagcg cccggcgggg gcgatctcaa ggctgcgtga
     2221 ctgctcgtgc cgctcgccgc tcatattgcc gcgcgcaatg cgcaaagccg ccgcgagctc
     2281 ggttgaattg accggctcgg tcagcgtggt gacgaagcga ttccgcgtaa ccggcggcgg
     2341 caggccttcc gtgcgttcct cggtgacaag gacgagcgca ggtgcgcagc cgagattgtc
     2401 accggcgatg cgcctgaacg tcggctcgtc cgcgttcgcg cacgcctggc gttgaacgat
     2461 ggcaatcggc cggcgcacgc cgtcggcccg cagcgcggca agcgcgacgg cggcttcgtc
     2521 catatcggca accaccttgg cgatgggcac gcacgaactc acgacgctat gcagattttc
     2581 gccgccgccg acgaccacga ccggcgtggg cggcagatag aaatgcggat aatcctgagc
     2641 ctgcggcttg aaatcgatct cgaaccagaa cgtgctgccc ttttcggggg cgctttcgac
     2701 gccgatcctg ccgccttgca gttcgacgag ctgcttggcg atggcaagac caaggccggt
     2761 gccgccgaag cggtcgatga ttgtctcgtc ggcctgggta aagcgatcaa aaatacggct
     2821 ctgcgcctcc ggactgattc cgatgcccgt gtcggtgact tcgaaacgca ggcgcacgcg
     2881 tccgttatcg cgcgcgacgg catcgacagc gatggtcacg tatccgtgct cggtgaactt
     2941 gacggcattg ccggcaagat tcgtaagcac ctcctcgagg tgatttttat cgccgacaag
     3001 gagttgcggc gtccgtgcag tgcaatggaa cgtaagcccg acgttcttgc gctgtgcttc
     3061 cagcgcaagc atgctgcgga tttcgctgag catggagacg agatcgaaat cttcggcctt
     3121 gacggcgatg cgtccggact cggcgcggga gaagtcgagg atcgagttga tcaatgcgag
     3181 aagctgccgg cccgacttgc cgatcgtctc cgacatctcc tcctgctccg agtccagcct
     3241 ggttttgccc agaaggtcgc tcaggccgat gatcgcattg agcggtgtgc ggaactcgtg
     3301 actgatgctg gcaagaaatg ctcccttggc acggtttgcc tcttccgcct gacgcttggc
     3361 ctgcgacagc ttgcggatca aggtcgagac atagagcggc agcaggatca ggccagccat
     3421 caggccggcg gccagtccgc ggtgctcgtg ccagaatggc gcactgacga tggcgattcc
     3481 gaaaccgacg acgctggttg ccgtggcggc aaacaggtaa ggcacgccga agcggaagcc
     3541 gttgccgaag atggtccaca ggtagatcgg atagagcggt gcaaacgcct cgccgccgac
     3601 gtacatgccg tacgagaaca ggccgaggtc tgaaatcatg ccgatcagcc gacgtccgac
     3661 cgaaatgccc ggccggcaca gcaggtgaac gaacagcgaa attgaaacga tctcgtaaag
     3721 cgcaaaatac agccactgct cacgtagcat ctgcgcggcg tctgtccggc cgaacatcgc
     3781 cgccacaatc agatacatga aagcggtgcc gctgagcgcg aggcgattga ctgtcagctc
     3841 gtgctcggaa tcggggcgat tgcgcagccg gcttgcgacc caggacaaga gggcccggac
     3901 acggaagccg ggcatcccgg gcacgaaggc ttttcggaag aaccccgtgt tcatcgtcag
     3961 cctcaagcgc tgcgtgcccg tgcgcgattt tcctgaacga atgcgatcag atcttcggcg
     4021 ctcatcggtt cgccgaaata aaagccctgg acctcgtcac agccttctgc gcgcagcatc
     4081 gttgtctgct gcaccgtttc gacgccttcg gcgacgattt cgaggtccag actgtgcccg
     4141 aggctcacga tcgcgcgcac gatcgccgca tcgctcgggt tggtcgtgat gtcgcgcacg
     4201 aaacactggt cgattttcag ccgatcgaca gggaaatgct tgacgtaagc gagcgtcgaa
     4261 tagcgggtac cgaagtcatc gatggagatg ccgacgccca gctcgcgcag ctgctgaagg
     4321 tccttcgaca cggcgtcggc gttgtgcatc acgatgcttt ccgt
//
LOCUS       DASSRL010000173         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1422201, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000173 DASSRL010000000
VERSION     DASSRL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422201"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFL49_03645"
     CDS             <1..942
                     /locus_tag="VFL49_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET7911099.1"
                     /translation="IIRNTSGIVCAPLGAEQARRLHLDPMVAKNEAPLGTAFTVSVDV
                     RHGLTTGISAEERCNTVRALANDNSGAADFVRPGHVFPLVARQGGVLMRSGHTEACVD
                     LCRLASLPPVGVLAELMNDNGSVMRGPEVVVFAKKHHLAQVSIAELIAYRQSRDKLVE
                     RVGEFQVASEIGALQGYAYLTPFDRVHHMAFVYGNIAGGKDIPARLHRADVIGDVFGG
                     ARSIRASLARFKKAGRGVIVYLRDGTAGVPVADIPHGDTETDAARSQQWREIGLGAQI
                     LKDLGISSIRLLASSKRTYVGLGGFGIEIVTTETLES"
     gene            988..1824
                     /locus_tag="VFL49_03650"
     CDS             988..1824
                     /locus_tag="VFL49_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HET7911100.1"
                     /translation="MGRWFRNLAVIAAVSASGLVLAASAAPPKFADTRASAIPVDVEL
                     VIAVDVSYSMDPDEQALQREGYVLALTSKEFLQALRQGAHGKIAIIYFEWAGQFDQKI
                     LMPWRLIDGPESADAVAAEIARAPYRRASRTSISGALHFAKPLFDDSGYKGLRRVIDV
                     SGDGANNAGPLVDLTRDDVLAAGITINGLPIMLKRPYTGTMDIAELDVYYEDCVIGGP
                     GSFVIPIREREKFIEATRTKLVLEVAGRQPVSRIVPAASRGRISCTIGEKMWQERWGG
                     YQ"
     gene            complement(1825..>2629)
                     /locus_tag="VFL49_03655"
     CDS             complement(1825..>2629)
                     /locus_tag="VFL49_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7911101.1"
                     /translation="IDDCPILAPGLSGSLSAAWAIADALKNTAKPLDIQTTATDSGLD
                     IDVRGSGTVDAERLAALAQVAEAHKLARLTRHGELVAQRTQPLLRIGRADVPLPAGAF
                     LQATVEGENTLARRMIEHVGKAKRVLDLFTGIGTFALRLAESAQVTAYDSEPAAISAL
                     QHAARKTSGLKPVDAQVRDLFRRPLMAAELKGLDAVVFDPPRQGAEAQAREVGKSKVP
                     VVVAVSCDAGTFARDAKILVTAGYKLAAAVPVDQFRYSHHVEIVAKFIQ"
BASE COUNT          460 a          799 c          853 g          517 t
ORIGIN      
        1 attatccgca acacgtcggg gatagtctgc gcgccgctcg gcgccgagca ggcgcgccgc
       61 ctgcatctcg atccgatggt ggcgaagaac gaagcgccgc tcggtacggc gtttaccgtc
      121 tcggttgatg ttcgtcacgg gctcacgacg ggaatctcgg ccgaggagcg ctgcaatact
      181 gtgcgcgcat tggccaacga caacagcggc gcggctgact ttgtccggcc tggccatgtg
      241 ttcccgctgg ttgcacggca gggcggggtg ctgatgcgat ccggccatac cgaggcctgt
      301 gtcgatcttt gccggcttgc ctcgctgccg ccggtcggcg tgctggccga attgatgaac
      361 gacaacggtt cagtgatgcg cggaccggaa gtcgtcgtct ttgcgaaaaa gcaccacctg
      421 gcccaggttt cgattgcgga attgatcgcc taccggcaga gccgcgacaa gctcgtcgag
      481 cgggtgggcg aattccaggt cgcctccgag atcggcgcgc ttcagggtta cgcctacctc
      541 acgccgttcg atcgcgtgca ccatatggcc ttcgtctacg gaaatatcgc gggcggcaag
      601 gatatcccgg ccaggttgca tcgcgccgac gttatcggcg acgtcttcgg cggcgccagg
      661 tcgattcgtg cttcactcgc acgcttcaaa aaagcggggc gcggcgtgat cgtgtatctg
      721 cgcgacggca cagccggcgt cccggtcgcc gatattccgc atggcgatac cgagaccgat
      781 gcggcgcgct cgcagcaatg gcgcgagatc ggtttgggtg cgcaaatcct caaagatctt
      841 ggtatttcct cgatccggct gctggcgtcc agcaagcgca cctatgtcgg tctcgggggg
      901 ttcggcatcg aaatcgtgac cacggaaacg ctggaaagct gagcaaccgg cctttttttc
      961 gccgatgccg catgccagca ttcggccatg gggcgatggt ttcgtaatct cgctgtcatt
     1021 gctgcggttt ccgcatcagg cctcgttctg gcggcctcgg cggcgccgcc gaagtttgcc
     1081 gacacgcgcg ccagtgccat ccccgtcgat gtcgaactcg tgattgccgt cgacgtttcc
     1141 tattccatgg atccggacga gcaggcgctg cagcgcgaag gctatgtgct ggcgctcaca
     1201 tcgaaagaat tcctccaggc gctgcgccag ggggcgcatg gcaagatcgc aatcatttat
     1261 ttcgagtggg ccgggcaatt cgaccagaaa atcctgatgc cgtggcgctt gatcgacggt
     1321 ccggaatcgg ccgatgcggt ggccgccgaa attgcccgcg ctccgtatcg gcgggcctcg
     1381 cgcacatcga tttccggtgc gttgcatttt gcaaagcctc tgttcgacga cagcggatac
     1441 aagggcctgc ggcgcgtgat cgatgtgtcg ggcgacgggg ccaacaatgc cggaccgctc
     1501 gtcgacctta cgcgcgacga tgttcttgcc gccggcatca cgatcaacgg cctgccgatc
     1561 atgctcaagc ggccttacac gggaacgatg gatatcgcgg agctcgacgt ctattacgag
     1621 gactgtgtca tcggcggacc ggggtcattt gtcattccga tccgcgaacg cgagaagttc
     1681 atcgaggcga cgcgcacaaa gctcgtactg gaggttgcag gccggcagcc tgtatcacga
     1741 atcgtgccgg cagcatcgag agggcgcatt tcctgcacca tcggcgagaa gatgtggcag
     1801 gagcgctggg gcgggtacca gtagctattg gatgaatttc gccacgattt ccacgtggtg
     1861 cgaatagcgg aactggtcga cgggaactgc cgcagcgagc ttgtagccgg cggtgaccag
     1921 aattttcgca tcgcgcgcaa aggtgccggc atcgcaagac accgcgacga caacgggcac
     1981 cttgcttttg ccgacctcgc gcgcctgtgc ttcggcgcct tgccgtggcg gatcgaagac
     2041 cacagcgtca agacctttta attccgcggc catcagcggt cgcctgaaaa gatcgcgcac
     2101 ctgtgcatcg accggcttga gaccgctcgt ctttcgggcc gcatgctgaa gggcactgat
     2161 cgcagcgggc tcgctgtcat aggccgtaac ttgcgcgctt tcggccaggc gaagcgcgaa
     2221 cgttccgatg ccggtgaaca gatcgaggac gcgcttggcc ttgccgacat gctcgatcat
     2281 ccggcgcgcc agcgtgtttt cgccttcgac ggttgcctgc aggaatgcgc ccgcaggcaa
     2341 cggcacatcg gcgcgcccga tgcgcaacag tggttgtgtg cgttgcgcaa ccagttcgcc
     2401 gtgacgcgtg aggcgggcga gcttgtgtgc ctcggcgacc tgcgcgagcg cggcgagccg
     2461 ctcggcgtcg accgttcctg atccgcgcac atcgatatcg agcccgctgt cggtcgcggt
     2521 cgtctgaata tcgagcggtt ttgcggtgtt cttgagcgcg tctgcgatcg cccaggcggc
     2581 ggacaaagaa cctgaaagtc caggcgccag aatcgggcaa tcgtcgatc
//
LOCUS       DASSRL010000174         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1737031, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000174 DASSRL010000000
VERSION     DASSRL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737031"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..515)
                     /locus_tag="VFL49_03660"
     CDS             complement(132..515)
                     /locus_tag="VFL49_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7911102.1"
                     /translation="MSLADKFNLFNEFNILRIICALFFIPHIIGKITVPATLEFFVKA
                     GFKPPATWMYIAGTIETFLCIGLFFGIYLQYVGVIAFIHLLVAGAATYKVTKSWIWVI
                     GGIEYCVFWAICCLVVAMHAYHAGI"
     gene            complement(607..1206)
                     /locus_tag="VFL49_03665"
     CDS             complement(607..1206)
                     /locus_tag="VFL49_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7911103.1"
                     /translation="MPRDTADRLAIRDLIENWVLWRDARMWDRFRTVWHKDGQMWATW
                     FQGSYEDFIRVSQEGYDRGVRIMHMLGGMSIDIKGKRAIAQTKMTISQRAMVEGVLCD
                     VVCSGRFYDFLEKRKGRWGIVLRRLAYEKDRIDPVEPDAKLALDKAHLAQFPEGYRHL
                     AYLQSKIGYRIKEELPGLDGPPLAALYARGEAWLKGKKL"
     gene            1311..2075
                     /locus_tag="VFL49_03670"
     CDS             1311..2075
                     /locus_tag="VFL49_03670"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7911104.1"
                     /translation="MPRLAGKTAIVTGGAKGIGRHYSQALAAEGARVMIADIADGRAL
                     AEEIAGRHGANSVASATFDVSDEKKVKALVAQTIERFGQIDVLVNNAALYSTLKPRTF
                     TEWDTELWDRVMAINVRGTYLMVRHVAPHMMSRRSGKIINIASGAPYKGVPRMLPYVT
                     SKGAILAFTRALSRELGEYGIAVNSLSPGYILSDTGLENTAHVEDERIPVRNSRAFRR
                     DAYPEDLLGALVFLASSDSDFVTGQSLVVDGGSVNN"
     gene            2090..2500
                     /locus_tag="VFL49_03675"
     CDS             2090..2500
                     /locus_tag="VFL49_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7911105.1"
                     /translation="MKVDGQCHCGHIRYEAEIDPDTVAICNCTDCQSLSGSVFRTVVP
                     TKKGSFRLLQGELKIYVKTGESGNKRPQSFCPHCGSPIYSTSTEPDPKVHSIRVGTLN
                     QRDQLIPKLQYWHRSAQRWTAHIGALPTFEKQRQ"
     gene            2607..>3279
                     /locus_tag="VFL49_03680"
     CDS             2607..>3279
                     /locus_tag="VFL49_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7911106.1"
                     /translation="MTLAIDTAQAGNKHNITGARQAYYDKISKYHLAPLWEVLKGLVT
                     PEPKTQMQPALWKFPEVESLMLEAGDVITAEEAERRVLVLENPGEPGKSRITNSLFAG
                     IQLILPGEIADAHQHVASAIRFVLKGKGAYTAVEGEKAQMEHGDFIITANWAPHDHGN
                     PGKEPVIWLDVLDMPTINHFQASFASHFDEKMQNINHGDGDSFERYATGVLPDGAPVH
                     LNRSPV"
BASE COUNT          699 a          981 c          975 g          624 t
ORIGIN      
        1 cgagccattg ttggagttgt actgacacat cggcgaaacc acgacccggt tcggcagggt
       61 caatccgcgc atggtgatgg gagaaaacag aacgctggcc attgggagga cttcctttca
      121 gtgcggagat ttcaaatacc ggcgtgataa gcatgcatcg cgaccaccag gcaacagatc
      181 gcccaaaata cgcaatattc gatgccgccg atcacccaaa tccagctctt ggtgactttg
      241 taggttgcgg cgccggcaac caaaagatgg atgaatgcga tgacgcccac atattggagg
      301 tagatgccga aaaacaaccc gatacagagg aaggtctcga tcgtccccgc gatgtacatc
      361 caggtcgccg gtggcttgaa gcccgccttg acgaaaaact caagcgtcgc cggcacggtg
      421 atcttgccga taatgtgcgg aataaagaac agcgcgcaga tgatccgaag aatgttgaac
      481 tcattgaaca gattaaattt gtcggcaagc gacatgtcag ttgtcccccg agtggttttg
      541 cgaccaacca cttgctttta aaatcgcact tcctgatgcg cgcgccaacc ccttgcgccg
      601 cccggtctaa agtttcttgc ctttaagcca agcctcaccc ctggcataaa gcgccgccag
      661 cggcggcccg tcgaggcccg gcagttcctc cttgatccgg tagccgatct tgctctgcag
      721 gtaggcgagg tggcggtagc cttccggaaa ttgtgccaga tgcgccttgt cgagcgcgag
      781 tttcgcatcg ggctcgacgg gatcgatgcg gtccttctcg taggcgagcc gccgcagcac
      841 gatcccccag cgccctttgc gcttttccag aaagtcgtag aagcgtccgc tgcacacgac
      901 atcgcacagc acgccctcga ccatcgcgcg ctgcgagatc gtcatcttgg tctgcgcgat
      961 cgcgcgtttg cccttgatgt cgatgctcat gccgcccaac atgtgcatga tgcggacgcc
     1021 gcggtcatag ccttcctggc tgacccggat gaaatcctca taggatccct gaaaccaggt
     1081 cgcccacatc tgcccgtctt tgtgccagac ggtgcggaag cggtcccaca tgcgggcatc
     1141 gcgccagagc acccaatttt cgatcaaatc tcggatcgca aggcggtcgg ccgtatcgcg
     1201 cggcatgatg gatcttcccc gtactcgctt gtgtgagctt cgtcccgttg caagaatgta
     1261 cgagcacacg aacgaccgcc acaaggccgt ggtgaacccg ggagaaatag atgccgaggc
     1321 ttgccggaaa aacggcaatt gtcaccgggg gtgccaaggg catcggtcgg cactactcac
     1381 aggcgctcgc cgccgagggg gcgcgcgtga tgatcgcgga catcgccgac ggccgcgcgc
     1441 ttgccgagga aatcgccggg cgccacggcg cgaattccgt cgccagcgcg acgttcgatg
     1501 tcagcgacga gaagaaggtg aaagcattgg tcgcccagac aatcgagcgg tttggccaga
     1561 tcgatgtgct ggtgaacaat gccgcgcttt attcgacgct gaagccgaga acgtttactg
     1621 aatgggacac ggagttgtgg gatcgtgtga tggcgatcaa tgtgcgcggg acatacttga
     1681 tggtccgcca cgtcgcgccg cacatgatgt cgcgtcgcag cggcaagatc atcaacatcg
     1741 cctcgggcgc gccttacaaa ggcgtgccgc gcatgctgcc ctatgtgacg tcaaaggggg
     1801 cgatccttgc cttcacgcgt gcattgtcgc gcgagctcgg tgaatacggc atcgcggtca
     1861 attcactctc gccggggtac atattgagcg ataccgggct tgagaatacg gcgcatgtcg
     1921 aggacgagcg catcccggtg cggaacagcc gtgccttcag acgtgacgcc tatccggaag
     1981 acctgctcgg cgccctcgta tttctcgcgt cgagcgacag cgatttcgtg accggccagt
     2041 cgctcgtggt cgacggcggg tcggtcaaca actgaaggag aaccgagcga tgaaggtcga
     2101 cggccagtgt cattgtggcc acatccgtta cgaagccgaa atcgatccgg atacggtcgc
     2161 gatctgcaat tgcaccgatt gtcagagctt gtctggctcg gtgttccgca cggtggtgcc
     2221 gacaaaaaag ggcagcttca ggctcttgca gggtgagctg aagatctacg tcaagacggg
     2281 cgagagcgga aacaagcggc cgcaatcttt ttgtccgcat tgcggctcgc cgatctactc
     2341 gaccagcact gaacctgatc cgaaagtcca ctcgatccgc gtcggtaccc tcaaccagcg
     2401 cgaccagctc atacctaaac tgcaatattg gcatcggtcc gcgcagcgct ggaccgcgca
     2461 cattggtgcg ctgccgactt ttgagaagca gcgtcagtag cctgactgct acatatccgc
     2521 tatgcgcctg ttttcacggg tttaagcggc gcaagcggtg ggttatattc gccgcaaatg
     2581 ggataatcag ttcacggagg agcccaatga cgctcgcgat cgacaccgcg caggccggta
     2641 acaagcacaa catcaccggc gcacgtcagg cctactacga caagatttcc aagtatcacc
     2701 tcgcgcccct gtgggaggtg ctcaagggcc tggtgacgcc agagccgaag acccagatgc
     2761 agccggcact atggaagttc cccgaggtcg agagtctgat gctcgaggcc ggcgacgtca
     2821 tcaccgccga ggaggccgag cggcgcgtgc tcgtgctgga aaaccccggc gagccgggca
     2881 agtcgcgcat taccaactcg ctttttgccg gcatccagct gatcctgccg ggcgagatcg
     2941 ccgatgcgca tcagcacgtc gcctccgcca tccgtttcgt gctcaagggt aagggagcct
     3001 acacggcggt tgaaggcgag aaggcgcaga tggagcacgg ggacttcatc atcacggcca
     3061 actgggcgcc gcatgatcat ggcaacccgg gcaaggaacc ggtgatatgg ctcgacgtgc
     3121 tcgacatgcc gacgatcaat cacttccagg catcgtttgc ctcgcacttc gacgagaaga
     3181 tgcaaaacat caaccacggt gacggcgatt ccttcgagcg ctacgcgacg ggtgtgcttc
     3241 ccgatggcgc gcctgttcac ctcaaccgct cgccggtca
//
LOCUS       DASSRL010000175         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_354577, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000175 DASSRL010000000
VERSION     DASSRL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354577"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..316)
                     /locus_tag="VFL49_03685"
                     /pseudo
     CDS             complement(56..316)
                     /locus_tag="VFL49_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607949.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(511..1620)
                     /locus_tag="VFL49_03690"
     CDS             complement(511..1620)
                     /locus_tag="VFL49_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911107.1"
                     /translation="MPNAAQQNREPDLDVALGESYYEGSRALEEQYTNEIVESIQRAI
                     AAHFRAGRGPARRDAHAYDNGCVRAIFQVDENLAADLCQGVFVRGHQYKAWIRFSNGN
                     SERRPAWFFDARGMAIKLTGVPGEKLMEDEKHTQDFILISHPRFFVDDLKRYKKTLVA
                     FLRGGYVDQLVVALVKLRPREAWFAFKANWNFMSNPLFQQYWSMTPYRFGVDPEKKFA
                     VKYTAKPRLHNKPGFFTRHLTRLGWNFSLKEEMNKTLAGNEVWFDFYIQRYVDAETTP
                     IEDSKVKWADEVSKPEPVGKIIIPIQDCMSPEQAVFCENLSFSPWHSLPEHRPLGLVN
                     RVRRIAYREISRLRHDLNHAPRQEPTGDEIFDLRS"
     gene            complement(1624..4494)
                     /locus_tag="VFL49_03695"
     CDS             complement(1624..4494)
                     /locus_tag="VFL49_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7911108.1"
                     /translation="MGDGILAYFGFPAAHEDSAERAVQATLEAIRLVSELSFEGVPRI
                     EMRVGIATGLVVVGDLIGEGPSREFALIGDAPNLASRLQQLARPNQILVAPATRRLLG
                     DLFEFEDLGDHDMKGFLEPIAVSGVLRRSKASRFEARRSSRIVPLIGRDAELAVLRDA
                     FDKARTGQGQLVAVSGEPGIGKSRLVNSFRHQLPPGSARVFLFQCSSYHLSSPWYPVI
                     RHVDDVLGSGYEARGSTKLERLEVLIDDISRTRRASIVPLLAPLLGISTEGHYPPLEL
                     TPQQQKQRTFLAMIEMLRAHCAQQPVILIAEDIHWIDHTSSQLLEMIRENIPNWRMLV
                     IATFRPEFRSLGEANAWINLNRLSPAQAASMVEAIDSGRKLPTTVVGQIISKTDGVPL
                     FVEEVTNTVLAGGTSEKSASNGVAWSPTIPETLHDSLMARLDQLASAKTVAQISAAIG
                     REFSFDLLEAIAPLPREDVRAAVDRLREAGLLSRREFSATETYAFKHALVQETAYSSM
                     LRSERQPLHLRIAETLSSKFVDVAEGAPEVVAYHFTQAREVTPAIHHWLKAGRQAGKR
                     SAFMEAITHFQTALKLLEELPADKTRIEVELQLQQALANASIAAKGFGAAETIVAFNR
                     ALDLCKQIGSSPQIFAVLSGLVGVHLMGGEIEKARAVAQELLALAQKSNDATALLMGH
                     RILGMSLFMLGELTASKEELEKAIALYDPAVHSPMAVIFSQDFKATAQAYLGLATVLC
                     GDAERGIAHSREAVDYAQELRHPHSICYVLSFLAGSYLIAGNPRAAFPVAERTVVQSN
                     EYGFPQWAAGGLMLRGWAHVEVGEIEAGLADIRNSIRGLEKTGTLIWMQFAHYLLARA
                     LVADHQWAKAKEIVERLLSEIGAAGGRWYEAEVRRLRGDILRGQHKPMSEIEACYESA
                     IATAHRQGARVWELKAMQSLDALHEAGASRRADAKRKPAVKI"
BASE COUNT          836 a         1434 c         1408 g          893 t
ORIGIN      
        1 tttgccttcc tcaatgaaaa tgacttttca aattctatcg attttttagt ggagcgggga
       61 ttttgtcctc aataccgagc ttgcaaaacc atctataggc caaattgacc tgcacctcgg
      121 cacagagccg ccgttccgag cggatcgcaa acatatagcc gacgatcagc attcggatca
      181 tcagcaccgg atcaatcgaa ggccggccgg tgtgcgagta gtaaggtgca agctctttgt
      241 gcacccagcc gaggtcgaga agaccattaa tctgccggac caaatggtcg ggaggtacga
      301 ctttgtcgag atcaatgaat aaaagaactg gccttgtccg tctttccgcc gccccatcat
      361 gcgcccgctc ccgccgatga tcgactcatc aatcgaacgg aatcacggtt cgacaactgt
      421 ctcaatgaga gtttgcaaca gaatcagggt aaagcggaca ggcgatcaag cgccgctggc
      481 atgatagaaa tttgccccaa tccgatcacg tcaggagcgg aggtcgaaga tctcgtcgcc
      541 ggtcggctcc tgccgcggcg cgtgattgag atcgtgccgc agcctgctga tttcgcgata
      601 ggcgatcctg cgcacgcggt tcaccaggcc caggggcctg tgctcaggca aggaatgcca
      661 cgggctgaac gacagatttt cgcagaaaac agcctgctcc ggtgacatgc agtcctggat
      721 tggaatgatg atcttgccga caggctccgg ctttgagacc tcatcggccc atttcacctt
      781 ggaatcctcg atcggcgtgg tctccgcgtc gacatagcgc tggatataga agtcgaacca
      841 cacctcgttt ccggccagcg tcttgttcat ttcttccttg agggagaaat tccagcccag
      901 ccgcgtgagg tggcgggtga aaaaacccgg cttgttgtgc aatcgcggct tggccgtgta
      961 tttgacggcg aactttttct ccgggtcgac gccaaaccgg tagggcgtca tcgaccaata
     1021 ttgctggaag agtggattgc tcatgaaatt ccaattggcc ttgaaggcaa accatgcttc
     1081 gcgcggccga agtttgacca gggcgaccac aagctgatcg acgtagccgc cgcgcaggaa
     1141 tgccacgaga gtcttcttgt aacgcttcag gtcatcgaca aaaaatcgcg gatggctgat
     1201 gaggatgaaa tcctgcgtgt gcttctcgtc ctccataagc ttctcgccgg gcacgccggt
     1261 caatttgatc gccatgccgc gggcatcgaa aaaccacgcc ggccgccgtt cggaattgcc
     1321 gttggaaaag cggatccagg ccttgtactg gtgcccgcga acgaaaacgc cctggcacag
     1381 gtcagcggcg aggttttcgt ctacctggaa aatcgcgcgc acgcagccgt tatcgtaagc
     1441 atgcgcgtcc cgccgcgccg ggccgcgccc ggccctgaag tgcgcggcga tcgcgcgctg
     1501 aatgctctcg acgatctcgt ttgtgtattg ctcctcgagc gcccggctgc cttcgtaata
     1561 gctttcgccc agggcgacat cgagatccgg ttcgcggttc tgctgggcgg cgtttggcac
     1621 ggctcagatt ttcaccgccg gtttccgttt ggcgtcggcc cggcggctgg caccggcttc
     1681 gtgcagcgca tcgagcgatt gcatcgcctt gagctcccac acgcgggcgc cctggcggtg
     1741 cgcggtcgcg atggcggatt cgtagcaggc ttcgatctcg gacatcggct tgtgctggcc
     1801 gcgcagaatg tcgccgcgca ggcggcgcac ttccgcttcg taccagcggc cgccggctgc
     1861 gccgatttcc gacagcagcc gctcgacgat ttccttcgcc ttcgcccatt gatggtcggc
     1921 aacaagcgcg cgcgccagca gatagtgcgc gaattgcatc cagatcagcg ttccggtctt
     1981 ttccagtccg cggatgctgt tgcggatatc ggcaaggccg gcctcgattt cgccgacttc
     2041 gacgtgcgcc cagcctcgca acatcagtcc gccggcggcc cattgcggga agccgtattc
     2101 gtttgactgg acaacggtgc gctcggcgac cggaaatgcc gcgcgtggat tcccggcgat
     2161 caaataggag cctgcgagaa acgaaaggac gtagcagatg ctgtgcggat gccgcagttc
     2221 ctgcgcgtag tcgaccgcct cgcggctgtg cgcgatcccg cgctcggcat cgccgcacag
     2281 aacggttgcg agcccgagat aggcctgcgc ggtcgccttg aagtcctgcg aaaatatcac
     2341 cgccatcggg ctgtgcacgg ccggatcata aagcgcgatc gccttctcca gttcctcttt
     2401 cgatgccgtc agttcgccaa gcatgaacag cgacatcccg agaatgcgat gccccatcag
     2461 cagcgccgtc gcgtcgttgc ttttctgcgc gagtgcaagc agctcctgcg ccactgcgcg
     2521 tgccttctcg atctcgccgc ccatcagatg gacgccgacc aggccgctga gcaccgcgaa
     2581 aatctgcggc gagctgccga tctgcttgca caggtcgagc gcccggttga aggcgacgat
     2641 ggtttccgcc gcgccaaatc ccttggcggc gatcgatgcg ttggcgagcg cctgctgcag
     2701 ctgcagctcg acctcgatcc gcgtcttgtc cgccggcagc tcttcgagaa gcttgagggc
     2761 agtctggaaa tgggtgatcg cttccatgaa ggccgagcgc ttgcccgcct gccgcccggc
     2821 cttcagccag tgatggatcg ccggcgtgac ctcgcgtgcc tgtgtgaagt gataggcgac
     2881 cacctccggc gcgccctccg cgacgtcgac gaatttgctg gagagcgttt cggcgatgcg
     2941 cagatgcaac ggctggcgtt cgctgcgcag catgctggaa taggccgttt cctgcaccag
     3001 cgcgtgcttg aatgcatagg tttccgtggc gctgaattcg cggcgcgaca aaaggccggc
     3061 ctccctcagc cgatccactg ccgctcgcac atcctcccgc ggcagcggtg caattgcctc
     3121 gagcaagtcg aaagaaaact cgcgtccgat ggccgccgat atctgcgcga cggttttcgc
     3181 ggacgccagc tggtcgagcc gcgccatcag cgagtcgtgc agcgtttccg gaatggtcgg
     3241 gctccaggcc acgccgttcg acgcgctttt ttcggacgtg ccgccggcca gaaccgtatt
     3301 ggtgacttcc tcgacgaaca gcggcacgcc gtcggtcttg gaaatgatct ggccgacgac
     3361 cgttgtcggc agcttccggc ctgagtcgat cgcctcgacc atcgaggcgg cctgggccgg
     3421 cgagagccgg ttcaggttga tccaggcatt cgcctcgccc agcgagcgga attccggccg
     3481 gaatgttgcg atcaccagca tgcgccagtt cgggatgttc tcccggatca tctccagcag
     3541 ctgcgacgag gtgtggtcga tccagtggat gtcttcggca atcaggatga ccggttgttg
     3601 cgcgcagtgc gcacgcagca tttcgatcat cgccaggaag gttctctgct tctgctgctg
     3661 cggcgtcagc tcgagcggcg ggtaatgtcc ctcggtcgaa atcccgagca acggcgcgag
     3721 cagcggcacg atcgatgcgc gccgcgtgcg cgaaatgtcg tcgatcagca cctcaagccg
     3781 ctccagcttg gtcgacccgc gtgcctcgta gccgctgccc agcacgtcgt cgacgtggcg
     3841 gatcaccggg taccacgggc tgctcaggtg gtaggacgag cactggaaca ggaacacgcg
     3901 ggcgctgccg ggcggcaatt ggtggcggaa gctgttgacg aggcgcgact tgccgatacc
     3961 cggctcgccg gagacggcga ccagctgccc ctggccggtc ctggctttgt caaatgcgtc
     4021 acgcagcacc gccagctcgg cgtcgcgccc gatcagcggc acgatgcgcg acgagcggcg
     4081 cgcctcgaac cggctcgcct tgctgcggcg caggacgcct gacaccgcga tcggctccag
     4141 aaaccctttc atgtcgtgat cgccaagatc ctcgaactcg aaaaggtcgc cgagcagccg
     4201 ccgtgtcgca ggcgcgacca ggatctggtt gggcctggcg agttgctgca ggcgggatgc
     4261 aagattcggt gcgtcgccga tcagcgcgaa ctcgcggctc gggccttctc cgatcaggtc
     4321 gccgaccacg accaggccgg tcgcaatgcc gacgcgcatt tcgatgcgcg gcacgccctc
     4381 gaaggacaat tccgaaacaa gtctgatggc ttccagcgtg gcctggacag cgcgctcggc
     4441 gctgtcctca tgcgcggcgg gaaatccgaa gtaggccagg atgccgtcgc ccatatatcg
     4501 gccgatccgg ccgtcaaatc cgaaaattgc ggtttcgcag gatctctgaa acgcgcgcat
     4561 gatgtcgcgc a
//
LOCUS       DASSRL010000176         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_949903, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000176 DASSRL010000000
VERSION     DASSRL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949903"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFL49_03700"
     CDS             <1..390
                     /locus_tag="VFL49_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7911109.1"
                     /translation="NIRATLVLFESGTRVASTLADLAGTLGSRPAAICRELTKLHEEV
                     KCEDLDALARFYAAGAETRGEFVILVAPPAEEQGADDIDALLKRALSRVSVKDAVGEV
                     AVATGRPRREIYQRALVLTKDGEDDSE"
     gene            377..778
                     /locus_tag="VFL49_03705"
     CDS             377..778
                     /locus_tag="VFL49_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET7911110.1"
                     /translation="MTQSEQPPRSVRREPLPERQAAFRVGISAESRAAAFLIAKGFRI
                     LARRWRSPLGEIDIVARRRHLLVFAEVKARASLDEAAESVNVRQRRRIAAAAEIWLAA
                     HPDDSIRDIRFDAILVTPGKIPRHIPGAFDA"
     gene            complement(779..2458)
                     /locus_tag="VFL49_03710"
     CDS             complement(779..2458)
                     /locus_tag="VFL49_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7911111.1"
                     /translation="MNLPKLSIAARLYTIFALMAATTVALSMVAVSNAHRHASLTEEF
                     ESANAGSWNVERINGLIYASTMETRGIYMSPGRAEAAKYADSLTKVTDQIGSVIAEWQ
                     RSVRNSDAAAFSDFSVRIAGYQDALYKLAPLAKQSGPQAAREWAENNLPAQVRAELTK
                     DLEKLSQHYSDRASQIYARIDEGIARTAMLLSVLACFAVILAIAGALTIARSVIKPIA
                     KITSITQAVAGGDGSIAIPFSERADEIGALAKSIGVFQNAMRSNEELNRTVRNDAEFR
                     ARRQEKMSNEISHFSADVEATLAELGRISDEMLAASAQLAAAADNASSKTARAEEASS
                     EASSNVRDIASAADELSASVNEIDRQVAQSNAIATKAVNEVESTNLAVKELDEAAARI
                     GDVVKLITDIAEQTNLLALNATIEAARAGEAGRGFAVVAGEVKALAGQTSRATEEIGA
                     QIAGMQRATQRSISAITGIEQTIREIGNISSAIAAAVTEQGAATQEIARGVEIAAKRT
                     VETAKEVNLMGTATQDTRSSAAAVKAVADDLGGAANRIRAQVDQFFERLSA"
     gene            2545..>2684
                     /locus_tag="VFL49_03715"
     CDS             2545..>2684
                     /locus_tag="VFL49_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET7911112.1"
                     /translation="MSLKIAVQMDPIERINIKGDSTFALLLEAQKRGHRLAYYTPDRL
                     ALL"
BASE COUNT          477 a          815 c          825 g          567 t
ORIGIN      
        1 aatattcggg cgacgttggt cctgttcgag agcgggaccc gcgtggcttc gactctggcc
       61 gatcttgcgg ggacgctcgg gtcgcgcccc gcagcaatct gccgcgaact gaccaagttg
      121 catgaggaag taaagtgcga ggatcttgat gcactggcac gtttctacgc ggctggcgcg
      181 gaaacacgcg gcgagttcgt aatcctggtc gcaccacccg ccgaagaaca gggcgcggac
      241 gatatcgatg cgcttctcaa gcgcgctttg tcgcgcgtct cggtcaagga cgcagttggc
      301 gaggtcgctg tcgcgaccgg acgtccgcga cgtgaaattt accagcgcgc actcgtactc
      361 accaaagatg gcgaggatga ctcagagtga acagccaccg cgctccgtaa gacgcgagcc
      421 actgccggag cgacaggcag cctttcgtgt cggtatttcg gcggagagcc gcgcggccgc
      481 ttttcttatc gcgaagggat tccggattct ggcgcggcgg tggcggagcc cgctcggcga
      541 gatcgacatc gtcgccaggc gccggcatct gcttgttttt gccgaggtga aagcgcgggc
      601 cagcctcgac gaggccgctg agtccgtcaa cgtgcgtcag cgccgtagaa ttgccgcggc
      661 agcagaaatt tggcttgcag cgcaccctga cgattcgatc cgcgatatcc ggtttgatgc
      721 gattttggtg acgccgggga aaattccacg gcacatcccg ggcgccttcg acgcgtgact
      781 atgcactcag ccgctcgaag aactgatcga cctgggcacg gattcggttt gcagcgccac
      841 caagatcgtc agccacggcc ttgaccgcgg cagcgctcga gcgcgtgtct tgcgtagccg
      901 ttcccatcag gttgacttcc ttggcggtct ccaccgtacg tttcgcggcg atctcgacac
      961 cgcgcgcgat ttcctgtgtt gccgcgccct gctcggtcac agcggcggcg attgcgctgc
     1021 tgatattgcc aatctcgcgg atcgtctgct cgatgccggt gattgccgag atcgaacgtt
     1081 gcgttgcgcg ctgcatgccg gcgatctgag ctccgatttc ctcggtggcg cggctcgtct
     1141 gcccggcgag cgctttcacc tcgccggcga ccacggcgaa accgcggcct gcctcgccgg
     1201 cacgcgccgc ttcgatcgtg gcattgagag ccagcagatt ggtctgctcg gcaatgtccg
     1261 tgatgagctt gacaacgtct ccgatacgcg cggccgcttc atcgagctcc ttgaccgcga
     1321 gattcgtcga ctcgacctca ttcacggctt tggtcgcaat ggcattggac tgggcgacct
     1381 gccgatcgat ttcattcacc gacgccgaaa gctcgtcggc agccgaagcg atgtcccgca
     1441 cattgctgct ggcttccgag gaggcctcct cggcgcgcgc tgttttgctc gaggcgttat
     1501 ccgccgcggc cgcaagctgc gccgatgccg cgagcatctc gtcggaaata cggccgagct
     1561 cggccagcgt cgcctcgaca tcggcggaga aatgggaaat ctcgttcgac attttttcct
     1621 gccggcgcgc gcggaactca gcgtcgttgc gcacggtgcg gttgagttct tcgttgctgc
     1681 gcatggcgtt ctgaaaaacg ccgatcgatt ttgccagtgc gccgatttca tcggcgcgct
     1741 cgctgaacgg tatcgcaatc gatccgtcgc cgcctgcgac cgcctgcgtg atgctggtga
     1801 tctttgcgat cggcttgatg acgcttcgcg caatggtcag cgcgccggca attgcgagaa
     1861 tgaccgcaaa gcatgcaagg acgctcagca gcattgctgt gcgagcgatc ccttcgtcga
     1921 tccgggcata gatctgactg gcccggtcgg aataatgttg gctgagtttc tcgagatcct
     1981 tggtcaattc ggcccgtacc tgagccggca gattgttctc tgcccattcg cgtgcggctt
     2041 gcggacccga ttgcttggcg agcggcgcca gcttgtacag ggcgtcctga tagccggcaa
     2101 tcctgacgga gaaatcgctg aatgccgcgg catcgctgtt acgcacgctg cgctgccact
     2161 ctgctataac ggagccaatt tggtcggtga ccttggtcag cgagtcggca tatttggcag
     2221 cttcggcgcg acctggagac atatagatgc cgcgtgtctc catcgtgctg gcgtaaatca
     2281 acccattgat gcgctccacg ttccagctgc cggcattggc ggattcaaat tcttcggtca
     2341 gcgaagcatg acgatgagca ttcgataccg cgaccattga cagcgccacg gtcgtggcgg
     2401 ccatcagtgc aaagatcgta tagagtcgcg ccgcaatcga aagcttgggc agattcacca
     2461 acggcccccg gccctgcaac aaatggtccc gaagcggcta tagtgcgggt aatttgttaa
     2521 cgctttgttc gcctaaaggt agtcatgagc ctgaaaatcg cggtgcaaat ggatccgatc
     2581 gaacggatca atatcaaagg cgactcgact tttgccttgc tgctggaagc ccaaaaacgt
     2641 ggccaccggc tggcttatta cacgcctgac cggctcgctt tgct
//
LOCUS       DASSRL010000177        10367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1317165, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000177 DASSRL010000000
VERSION     DASSRL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317165"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..2222)
                     /locus_tag="VFL49_03720"
                     /pseudo
     CDS             complement(106..2222)
                     /locus_tag="VFL49_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798874.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
     gene            complement(2248..2775)
                     /gene="petA"
                     /locus_tag="VFL49_03725"
     CDS             complement(2248..2775)
                     /gene="petA"
                     /locus_tag="VFL49_03725"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET7911113.1"
                     /translation="MTTVSSAEHTRRDFLYIATAAVGAVGAAAALVPLIAQMNPDAST
                     IAAGAPIEVDLGPIADGQIIKVFWRSKPIFIFHRTKKEIEEAQKVNVASLPDPEPDSA
                     RVKPGHDQWLVVIGICTHLGCIPLPHQGEYDGWFCPCHGSQYDTSGRIRKGPAPKNLF
                     LPPYKFVSDTKIQIG"
     gene            3127..3606
                     /locus_tag="VFL49_03730"
     CDS             3127..3606
                     /locus_tag="VFL49_03730"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET7911114.1"
                     /translation="MQMALYEPDIPQNTGTILRLCACLGIEAHIIEPSGFPVTDRAFR
                     RAGMDYIDNVSITRHGSFAAFEAWRQRQRLQLVLLTTAAERSYLDHAFTAGQVLLFGR
                     ESAGVPKTVHERADVRLRIPMRPGMRSLNIAMAAAMVAGEALRQARDLRGDRIVLTS"
     gene            3614..4567
                     /gene="hemF"
                     /locus_tag="VFL49_03735"
     CDS             3614..4567
                     /gene="hemF"
                     /locus_tag="VFL49_03735"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HET7911115.1"
                     /translation="MDQSALDTRKRTARAWFEALRDDICAALEALEDALPADAPFAEH
                     AAGRFVRTPWQRTNHGGAQSIPGLSESGTLSAGSAKADPDGGGGIMAMMHGRVFEKVG
                     VHCSTVHGEFAPEFRKEIPGAGNDPRFWASGISLIAHPHNPNVPAVHMNTRFVVTSKS
                     WFGGGADLTPVLDKRRVHNDPDSIAFHAAMKTACDAHAEIAPYDKYRKWCDEYFFLKH
                     RNEPRGVGGIFYDYLETDWDETFAFTQDVGRAFLRVYPNLVRKNFAQPWTDAEREEQL
                     IRRGRYVEFNLLYDRGTIFGLRTGGNVESILSSMPPVVKWP"
     gene            complement(4572..5330)
                     /locus_tag="VFL49_03740"
     CDS             complement(4572..5330)
                     /locus_tag="VFL49_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7911116.1"
                     /translation="MNVLSGFADISFWQLIPVALVALFASIVGGVSGYGTGALMPLVL
                     VPLVGAEPVVPIIAISAMFTNSSRVFAFMKYADWGRAAVVLVAAVPTCVLGAWGYTHL
                     SSAGAALVIGSMLIASVPLRRLLSHHGFRIGDTGLALGSLGYGLVVGGTAGSGVILLS
                     LLMSAGVEGAGVIATDAAISIVIGIVKISVFGLAGVITAQVIAFALLIGIVALPGAFL
                     AKAFVERMPVHVHTAILDAVVLLGGVFLVCSAVR"
     gene            complement(5327..6592)
                     /locus_tag="VFL49_03745"
     CDS             complement(5327..6592)
                     /locus_tag="VFL49_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET7911117.1"
                     /translation="MTPQAGRTERKFNNAAWLTSGPLPRLLAVLDSDGEEARAVGGAV
                     RNALLGAPVLEVDVATTAVPDEVVNRVTAAGFKVVPTGIDHGTLTVVINKHPFEVTTL
                     RKDVETYGRHAKVEFGRDWKADAERRDFTINALSVTRDGTVYDYAGGLDDLAHRRVRF
                     IGDPAKRIQEDYLRILRFFRFHAAYGAGHPDPAGLSACIAARKGLEQLSRERVRMELL
                     KLLVAPHATATLAVMSHAGLLLLVLGGVPYLADFENVAKVEAVTGAAPDALRRLGALG
                     VVIVEDAERLWQKLRLTNNEHERLVSMAEGWRRISSAFGENALKALLYRLNPQHYTDH
                     ALLGWARSEATAHDARWQELATLPQRWIAPEFPLKAADFMKRGLAQGPSLGAALACAE
                     RAWIAAGFPQERRALDEIADAAAKNATSP"
     gene            complement(6592..6855)
                     /locus_tag="VFL49_03750"
     CDS             complement(6592..6855)
                     /locus_tag="VFL49_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HET7911118.1"
                     /translation="MIRPVLTELLLFLTPFVLYAVFLWATKSGVLDLKSWPVMHVVTL
                     AIAALLLVLGSFIYFAHFSGAPPGSTYIPAHMEDGKFVPGTTK"
     gene            complement(6852..7505)
                     /locus_tag="VFL49_03755"
     CDS             complement(6852..7505)
                     /locus_tag="VFL49_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET7911119.1"
                     /translation="MQHPLTALSAGEFFARVRARLTLDVPPGLSDPNITPARGDHDAD
                     PVMRKIAEVRPIRPAAVLVPVVDHPEPAVLFTQRAQHLPDHAGQVSFPGGKIDEGDTD
                     PRASALREAEEEIGLDRGHVEPLGYLDLYMTTLGYRIVPLIARVKPGFVLKLNPREVD
                     ATFEVPLDFLMDQSNVQRHSRDWQGMTRHYYAVTFGERYIWGVTAGILRNLYDRIYR"
     gene            complement(7505..8092)
                     /locus_tag="VFL49_03760"
     CDS             complement(7505..8092)
                     /locus_tag="VFL49_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HET7911120.1"
                     /translation="MAKAGQGGLEGIAAGLPRENKGLPPVELWNPPYCGDIDMRIAPD
                     GTWLYQKTPIGRPALVKLFASVLKREGDKHFLVTPVEKVGIVVEDAPFLAVEMKTMRN
                     DSGQVLQFRTNVDDWVTAGPGHALRFELQPETSGLKPYLHVRRDLWAKVTRALFYDLV
                     ALGEERDVGGKAMFGVASQGEFFAMAEASAVREFA"
     gene            8288..9298
                     /locus_tag="VFL49_03765"
     CDS             8288..9298
                     /locus_tag="VFL49_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7911121.1"
                     /translation="MAKAGGESLEKLEDMIVRAAESTAAHVRAAKAAIGAVIFGQDMV
                     VERALVTVLSGGHALLVGVPGLAKTKLVDTMGTALGLDARRIQFTPDLMPSDILGTEV
                     LEESPGGKRSFRFIAGPVFAQLLMADEINRASPRTQSALLQAMQEQHVTVAGVRHDLP
                     KPFHVLATQNPLEQEGTYPLPEAQLDRFLMEVDVDYPDQDAERKILFDTTGAEESRAK
                     PAMTAEDLLSAQRLVRRLPVGESVVEAILALVRSARPGPEAGDIGKLISWGPGPRASQ
                     ALMLAVRARALLDGRFAPSIDDVLDLAEPVLKHRMALTFAARAEGETIPNVIRRLKAR
                     IG"
     gene            9304..10257
                     /locus_tag="VFL49_03770"
     CDS             9304..10257
                     /locus_tag="VFL49_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7911122.1"
                     /translation="MAQPKPNDIEAGTVKQATVGARKLADAMPRLILEARRVASTVIH
                     GLHGRRRAGPGENFWQYRRFVSGEPAQNVDWRRSARDDHLYVREREWEASHTVWIWPD
                     RSPSMTFSSALAKDSKLDRALVVAFALAEVLVQGGERVGVPGLTRPTASRNVIEKMAE
                     AILHDPAERASLPPSFAPSPLSEVVLLSDLWVPMGEVRSTITQLSATGAHGHVVQIVD
                     PAEESFPYSGRVEFIEPEGAGSITAGRAEAWRNDYQAIVERHRAAVRAETDLRGWSFA
                     IHRTDRPASELLLVLHSRMGDNTQPFVVSSRHATASPASAA"
BASE COUNT         2130 a         3235 c         3142 g         1860 t
ORIGIN      
        1 ctgccggccg ggtgcgggat tcctgtctgg aagataagca agacgtcgat ggccgggaca
       61 agcccggcca tcgcagtttc aacgttaaat gacgcgttgc ggattctaga tcgtcgtctt
      121 cttcgggtcc tggccgtgcg cgacctcgcg cggcttttca acctcgtgcc agaccttctt
      181 cttggtgaag tacatcaggc ccgccagcac gatcaggaaa atcaccacct gaaggcccag
      241 ccgtttccga gccacgagat ggggctccgc cacccacatc aggaacgcgg aaatgtccct
      301 ggaatactga tccagggtct gtggcgagcc gtcatcatag gttacctggc catcctgcaa
      361 cggcggcggc atcgcgatgt tgtggccggg gaaatatttg ttgtagtggc cgttcggcgg
      421 cagctggaag cccggcggcg ggttttcata gcctttcagc agcgcggcaa tgtagtcggg
      481 gccctgctcc tgatactgcg tgaatatgtc gaatacgaac cacggaaaac cgcgctgata
      541 agtgcgggct ttcgcgagcg tcgacatgtc gggcggtgcc acgccgtgca ccgctttcgc
      601 ggcaagctcg ttcgggaacg gcgacgggaa gtaatcggcc ggccggccgg gacgctcggt
      661 gggctgcccc ttgtcgtcga gatcctgaat cctggcctct gacgcgacag ccgcagcctg
      721 cgcttcgctg aatccgggac cacccggctc cgcgagattg cggaatgaaa ccatcgacat
      781 cgaatgacag accgcgcaga cttccttgta aatcttgaag ccgcgctgca gctgcgcctg
      841 atcgaatttc ccgaatggcc ccgcgaacga ccatttaagt cttggcggag gaacctgctc
      901 ttcggctgac gcgggcagaa ccagtgcggc aaccgcgccg gccaaggcaa aggcaacaat
      961 ggtgcgtttc attattctca ttgccctgcc tccgctcacg ccttcgcgcc cagcaccgat
     1021 tccgaaatcg agttcggcaa cggcttggtt ttttcgaaga tgccgagcag cggcatgacg
     1081 atcaggaaga aggcgaagta gtacaccgtg aatatgcgcg acagaatgac gtaaattccc
     1141 tcgggctgtt tggagccgag ccagccgagg ccgatgcaag cgatcaccca gagccagaag
     1201 aactgcttga agagcggacg gtaacgcgcc gagcgcaccc gcgaggtatc gagccagggg
     1261 ataaatgcaa ggatgatgat cgaggagaac agcgcgatca cacccatcag cttgtcagga
     1321 atcgaccgca ggatggcgta gaacggcagg tagtaccact cgggaacgat ttccgccggc
     1381 gtcacggccg gattagccgg gatatagttg tcggcatgtc cgaggaaatt cgggatgtag
     1441 aagacgaacc acgcgtaaac gatgcagaag acgaccatga agaagccgtc ctttacggtc
     1501 gcgtaaggcg taaacggcac cgtgtccttg tccgacttgg gctcgatacc ggtagggttg
     1561 ttctggccgg cgacatgcag tgcccagatg tggagcacta caacgcccgc aatcacaaac
     1621 ggcagcaagt agtgcagtga gtagaaacga ttcagcgtcg gattgccgac cgcaaaaccg
     1681 ccccacagcc aggtgacgat agcatcgccc acgccgggga tggcagagaa caggttggtg
     1741 atcacggtcg ccgcccagaa gctcatctgg ccccagacca gcacgtagcc catgaagccg
     1801 gtcgccatca tcagcagata gatgatgacg ccgagaatcc acagcacttc acggggctct
     1861 ttgtatgacc cgtagtaaat gccgcggaac atgtggatat agaccgcaag aaaaaacatc
     1921 gcggcgccgt tcgcgtgcag atagcgcaac agccaaccga agttgacgtc gcgcatgatt
     1981 ccctcgaccg atttgaaggc gagatcgaca ttcggcgtgt aatgcatcgc cagcacaaca
     2041 ccggtgatga tctgcacgcc aagcatgaat gcgaggatgc cgccaaaggt ccacaagtaa
     2101 ttcaggttgc gcggcgtcgg gtacgcaacg aacgaagaat gaatcaggcc gccgagtggc
     2161 agccgtttct cgaaccattg cagaaatttg ttctgcggtt ggtaggtcga gtgtccgctc
     2221 atgattgaac cttgaatgcg atttcgctca gccgatctgg atcttggtgt cggaaacaaa
     2281 cttgtagggg ggcaggaaca ggtttttcgg tgcaggcccc ttccggatgc gccccgacgt
     2341 gtcgtactgc gacccgtggc aggggcagaa ccagccgtca tattctccct gatgggggag
     2401 cggaatgcag ccgaggtgcg tacaaatgcc gatgaccacg agccactgat cgtggcccgg
     2461 cttgacgcgc gccgaatccg gttccggatc gggcaggctt gcaacgttga ccttctgcgc
     2521 ctcttcgatt tcctttttgg tacgatggaa aatgaagatc ggcttcgagc gccagaatac
     2581 cttgatgatc tgtccatcgg cgatcggccc gagatcaact tcgatcggcg caccagccgc
     2641 aatggtcgag gcatccggat tcatttgagc gatcagcggg acgagagccg ctgcagctcc
     2701 caccgcaccg acggccgcgg tggcgatata aagaaagtcg cggcgcgtgt gctccgccga
     2761 agacacggtc gtcacgtctt tagccctctc ctccggtggc ttgttcatcc gcccgaggct
     2821 ggaacccggg accgaagtcc gaagggcggc ggggcggacc ggttgcggaa accctggaaa
     2881 aggcagggca acgcttcggt ggagttctat tggcaccctt gccgtaagag cgcaagcccg
     2941 caaaagcgcg gctggcaaaa gtgggcaccg gttttgcgct cggccgcact tcagatgtga
     3001 cattatgcgc gcaataaccg ggtaaccggt acccatttgg cccgattgcg gtgcccgcga
     3061 gaattagccg cataaatcat ttgtctacag cggcttcttg ccggcgacga ggaaaaccga
     3121 cccgcgatgc agatggcact ttatgagccg gatatccccc agaacaccgg gacgattctc
     3181 cggctgtgcg cctgcctggg catcgaggcg cacattatcg agccaagcgg atttccagtg
     3241 accgatcgcg ccttccgccg cgccggcatg gactacatcg acaacgtcag catcacccgt
     3301 cacggctcgt ttgctgcctt cgaggcatgg cggcagcgcc aacggcttca gcttgtgctt
     3361 ctgaccacgg cggcggagcg gtcctacctc gatcatgcgt tcaccgcagg ccaggtgctg
     3421 ctgttcggcc gcgaaagtgc cggcgttccg aaaaccgtcc acgagcgggc agatgtccgt
     3481 ctgcgtatcc cgatgcggcc cggcatgcgc tcacttaata tcgccatggc ggcagcgatg
     3541 gtggccggag aggccctgcg tcaggcgcgg gatttgcgcg gtgataggat cgtcctaacg
     3601 agttaatgag aaaatggatc aatccgcact cgacacccgc aagaggacgg cgcgcgcctg
     3661 gttcgaggcg ttgcgtgacg atatctgcgc cgcactcgag gccctggaag acgcgctgcc
     3721 ggcggatgcc cctttcgccg aacacgctgc aggccgcttt gtccgcaccc cctggcagcg
     3781 cacgaatcat ggcggcgcgc agtcgatccc gggtttatcc gagagcggca ctctaagtgc
     3841 cgggtcggct aaagccgacc cggatggagg cggcggcatc atggcgatga tgcatggccg
     3901 ggtattcgag aaggtcggcg tgcactgctc gaccgtgcac ggcgaattcg cgccggaatt
     3961 ccgcaaggaa atacccggcg ccgggaacga cccgcgcttc tgggcgtccg gcatttcgct
     4021 gattgcgcat ccgcacaatc cgaacgtacc ggcagtccac atgaacacac ggttcgtcgt
     4081 gaccagtaaa tcctggttcg gcggcggcgc cgatctcacg cctgtcctcg acaaacgccg
     4141 cgttcataac gatccggaca gcattgcttt ccatgccgca atgaagacgg catgcgacgc
     4201 gcatgccgaa atcgcgccct acgacaagta caggaaatgg tgcgacgaat actttttcct
     4261 caagcaccgc aatgagccgc gcggcgtggg tggcatattc tacgactatc tggaaaccga
     4321 ctgggacgaa accttcgcct tcacgcagga tgtcggacgc gcattcctgc gcgtctaccc
     4381 aaatctggtg cggaaaaatt ttgcccagcc ctggaccgat gccgagcgcg aggagcagct
     4441 gatccggcgc ggccgttacg tcgaatttaa cctgctctac gatcgtggta cgattttcgg
     4501 actacgtacc ggcggcaacg tggaatcgat cctttcctcg atgcccccgg tcgtgaaatg
     4561 gccgtgaccg gtcagcgcac cgcgctgcaa accaggaaca caccacccag cagcaccaca
     4621 gcgtccagga tcgccgtgtg aacgtgtacc ggcatccgct caacaaaagc cttcgccaga
     4681 aaggccccgg gcagagccac aattccgatc aacagcgcaa aggcgatcac ctgtgcagta
     4741 atgactccgg ccagtccgaa gacagaaatc ttcacgatcc cgatcacgat ggaaatggcg
     4801 gcatcggtcg cgataacgcc cgcgccttcg actccggccg acatcagcag cgagagcaag
     4861 atcacgcccg atcctgcagt gccgcccacc acaagaccat agcccaacga gcccagcgca
     4921 agaccggtgt cgccgatcct gaaaccgtga tggctcagca gccgcctcag cggcacgctc
     4981 gcaatcaaca tgctgccgat caccagcgct gcgcccgcgc tggagagatg ggtgtagccc
     5041 caggcaccca gaacacatgt cggcaccgcg gccacgagca cgaccgcggc ccggccccag
     5101 tcggcatatt tcataaatgc aaagacgcgg ctggaattgg tgaacatcgc agaaatcgcg
     5161 ataatcggca cgaccggctc ggcgccgacc aagggcacca gtaccagcgg catcaacgcg
     5221 ccggtgccat aaccggagac gccgccgacg attgaagcaa atagcgccac cagggcgacc
     5281 gggatcaatt gccagaatga aatgtcggca aacccggaaa gcacattcat ggactggtcg
     5341 cgtttttcgc ggcagcgtcc gcaatttcat caagcgcgcg tcgctcttgc ggaaatcccg
     5401 ccgcgatcca tgcacgctcc gcgcaggcaa gcgccgcgcc caggctcggg ccctgcgcca
     5461 ggccgcgctt catgaagtcg gccgctttca agggaaattc cggtgcaatc catcgctgcg
     5521 gcagcgttgc aagctcttgc cacctggcgt cgtgagcggt cgcctcggag cgcgcccatc
     5581 caagcaaggc gtgatcggtg tagtgttgcg gattgaggcg atagagcaac gcctttagcg
     5641 cattttcgcc gaatgccgac gaaatgcgcc gccagccttc cgccatcgac acaagccgct
     5701 cgtgctcatt gttcgtgagc cgcagcttct gccacaaccg ttcggcatcc tcgacaataa
     5761 cgacgccgag tgctccaagg cggcgcagcg catccggcgc tgctccggtg accgcctcga
     5821 cctttgcgac gttctcgaaa tcagcgagat agggcactcc gccgagcaca agcagcagca
     5881 gcccggcatg cgacatgacg gccagggtcg ccgtcgcgtg tggagcgacg agcagtttca
     5941 gaagctccat gcgcacccgc tcgcgcgaaa gttgctcgag tcctttgcgt gccgctatac
     6001 aggcagaaag accggcggga tcaggatggc cggcgccata ggcagcgtga aagcggaaga
     6061 agcgcaaaat gcgcaagtag tcttcctgga tgcgtttggc gggatcgcca atgaatcgca
     6121 cgcggcgatg agcaagatcg tcaagcccgc cggcatagtc gtagacggtc ccgtctcgcg
     6181 tgaccgacaa cgcattgatc gtgaagtcac gccgctcggc atccgctttc cagtcacggc
     6241 cgaattcaac ttttgcatga cggccgtagg tctcgacatc cttacgcagt gtcgtgactt
     6301 cgaaaggatg cttgttgatc acgacggtca acgtaccgtg atcgatgccc gtcggaacca
     6361 ccttgaatcc cgccgcggtc acgcgattca caacttcgtc gggcacagcc gtcgtggcga
     6421 cgtcgacctc caggaccggc gcgccaagca gcgcgttgcg aaccgcccca ccgacagcgc
     6481 gcgcctcctc gccgtcgcta tcgagcacag ccagcagccg cggcaacggc ccggacgtca
     6541 gccaggcggc attgttgaat ttgcgttcgg tgcggccggc ctgtggcgtc attacttcgt
     6601 ggtccctggc acgaattttc cgtcttccat atgagccggt atgtaagtcg atccaggagg
     6661 tgcacccgag aagtgcgcga aatagatgaa gctgcctagc accagcagca gggccgcaat
     6721 ggcgagcgtg acgacgtgca tcaccggcca cgacttgaga tccaggacgc cggacttcgt
     6781 cgcccacaga aaaaccgcgt agagcacgaa cggcgtgaga aaaagaagaa gctcggtgag
     6841 aaccgggcgg atcatcgata aatccgatcg tagagattcc gcaggatacc agccgtcacg
     6901 ccccaaatgt agcgctcgcc gaaagtgacc gcgtagtagt gccttgtcat gccctgccaa
     6961 tcgcgcgaat ggcgctgcac gttgctctga tccatcagga aatccagagg cacctcaaag
     7021 gtcgcatcga cttcgcgggg gttgagtttg agcacgaagc ccggtttgac gcgcgcgatc
     7081 agcgggacaa ttcgatagcc gagcgtggtc atatagagat caagataccc aagcggctcg
     7141 acgtgcccgc gatcgaggcc gatctcttcc tcggcttcgc gcaaggccga tgcgcgcgga
     7201 tcggtatcac cctcgtcgat cttgccgccc ggaaatgaaa cctgtccggc atggtccggc
     7261 agatgctgcg cgcgctgcgt gaacagcacc gccggttcgg gatgatcgac gaccgggacc
     7321 agcaccgcag cggggcggat cggcctcact tcggcgatct tccgcatcac cgggtcggca
     7381 tcgtgatcgc cacgagccgg cgtaatgttc ggatcgctaa ggccgggcgg cacgtcgagc
     7441 gtcaatcgtg cccgcacacg cgcgaagaat tctccggccg aaagggccgt cagcggatgt
     7501 tgcatcaggc gaactcccgc acggcgcttg cttccgccat cgcgaaaaac tcaccctgcg
     7561 aagcgacgcc gaacatcgcc ttgccgccga catcgcgctc ttcgccaagc gcgacaaggt
     7621 cgtaaaacag cgcgcgcgtg accttcgccc ataggtcgcg tcgcacatgg aggtacggct
     7681 tcagtccact tgtttccggt tgcaattcga aacgcaatgc gtgaccgggc ccagcagtaa
     7741 cccagtcgtc gacattggtg cgaaactgga gcacctgccc gctatcgttc cgcatcgttt
     7801 tcatttccac ggcgagaaac ggcgcatcct cgacgacgat cccgactttc tcgaccggtg
     7861 tcacaagaaa gtgtttgtcg ccttcccgct tgaggaccga ggcaaacaat ttgaccaaag
     7921 ccggccggcc gattggcgtt ttttggtaga gccaggtccc atccggggcg atcctcatgt
     7981 cgatatcgcc gcaatagggc ggattccaaa gttcgacggg cgggagtccc ttgttttcgc
     8041 ggggcagccc tgccgcgatc ccctccaacc ctccctgccc tgccttcgcc attgttttcc
     8101 ttcgataatt agggtgccgc gagcctgttc gcatcttcgt gattcacccg gctcggcgca
     8161 ggaagatagc gtaagcttgg ccggagattg aggcagattc ggcctaaata gcgcagcaaa
     8221 atcgcccttg gtgcgtttgt tgcatagtgc tggaagcacc aatcgggcga cgaaaggaat
     8281 tgagattatg gcgaaagccg gcggcgagag cctggagaaa ctggaagaca tgatcgtgcg
     8341 tgcggctgaa tccacggccg cccatgtgcg tgcggcgaaa gcggcgatcg gtgcggtcat
     8401 tttcggccag gacatggtcg tcgagcgcgc actggtcacg gttctttccg gcggtcacgc
     8461 gctgctggtc ggcgtgcccg gccttgcgaa aacaaaactg gtcgacacca tgggtacggc
     8521 gctcggtctc gacgcccgac gaatccagtt cacacccgat ttgatgccat ccgacattct
     8581 cggcacggaa gtgttggagg aaagcccggg cggaaagcgc agctttcgct tcatcgcagg
     8641 accggtgttc gcccaattgc ttatggctga cgaaatcaat cgtgcctcgc cacgcaccca
     8701 atcggcgctc ctgcaagcga tgcaggagca acatgtgacg gtcgccggcg ttcgtcacga
     8761 tctgccgaag ccgttccatg ttctggcaac acaaaatccg ctcgagcagg aaggtaccta
     8821 tcctctgccg gaagcgcagc tcgaccgctt cctgatggaa gtcgacgtcg actaccctga
     8881 ccaggacgcc gaacgaaaaa tcctgttcga tacgaccggc gcggaagagt cccgcgccaa
     8941 gccggcgatg accgccgaag acctgctgtc agcacagcgg ctggtccgcc gattgcccgt
     9001 cggtgagtcg gtcgtcgagg caattctcgc gctcgtacgt tcggcccgtc cgggacccga
     9061 agcgggcgac atcggcaaat tgatttcttg ggggccaggg ccgcgtgcca gccaggccct
     9121 catgcttgca gtgcgtgcac gcgcccttct cgacggccgc ttcgcgcctt cgatcgacga
     9181 tgtgctcgat ctggcagagc ctgtgctcaa gcaccgcatg gcgctgacgt ttgcggcgcg
     9241 agcggaaggc gaaaccatcc cgaacgtgat ccggcggctg aaggcgcgca tcggatagtt
     9301 gcaatggcgc agccaaagcc gaacgacatc gaggccggaa cggtcaaaca ggcgacggtc
     9361 ggcgcacgca agctggccga cgccatgccc cgccttattc ttgaggcgcg ccgcgttgct
     9421 tcgacggtca ttcacggatt gcacggccgg cggcgcgccg ggccgggcga gaacttctgg
     9481 cagtaccgcc gcttcgtatc cggcgagcct gcccagaacg tcgactggcg acgttccgcg
     9541 cgcgacgacc atctttatgt acgagaacgg gaatgggaag cctcgcacac ggtgtggatc
     9601 tggcccgacc gctcgccgtc aatgactttc agttccgcct tggcaaaaga cagcaagctc
     9661 gaccgtgcgc tggtcgtggc cttcgcgttg gccgaggtcc tcgtgcaggg cggcgaacgg
     9721 gtcggcgttc ccggcctgac ccggccgaca gcaagccgca atgtcatcga gaagatggcc
     9781 gaagcgatcc tgcacgatcc cgccgagcgt gcgagtctgc cgccttcatt tgcgccgtcg
     9841 cccctgtccg aggtcgtgct gctctccgat ttgtgggttc cgatgggcga agtgcgcagc
     9901 acgatcaccc agctttccgc caccggcgcg cacggccacg tcgtccagat tgtcgacccg
     9961 gcggaggaaa gtttccccta ttccgggcgt gtggaattca tcgagcccga aggcgccggc
    10021 agcatcacgg ccggtcgcgc cgaggcctgg cgaaatgact atcaggcgat cgtcgagcgc
    10081 caccgagccg ccgtccgcgc cgaaaccgac ctgcgcggct ggagctttgc gatccatcgc
    10141 actgatcgcc cggcaagcga gcttctgctc gtgctacact cccggatggg cgacaacacg
    10201 cagccgtttg tcgtcagcag ccggcacgct acggccagtc ccgcgagcgc cgcatgatcg
    10261 ggtcgattcc gctaggcttt gctcagccgc tggtgttgct cggccttctc agcctgcccg
    10321 ttttgtggtg gctcctgcgc ctcgtgccgc cgcgccccca acgtatc
//
LOCUS       DASSRL010000178         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_967232, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000178 DASSRL010000000
VERSION     DASSRL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967232"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..714)
                     /locus_tag="VFL49_03775"
     CDS             complement(211..714)
                     /locus_tag="VFL49_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7911123.1"
                     /translation="MTERQGNEMEGGCTCGAVRYRLRARPLFVHCCHCTWCQRESGSA
                     FAVNAFIEASQVELLQGELTRTTLPSASGKGQVFWRCAGCGVTVWSNYPQVGPAIHFI
                     RVGTLDDPSRVPPDIHIYTSTKQPWVVLPLGVPAVAEFYRPADHWPADSIARFKAARA
                     AIGIVSD"
     gene            886..2136
                     /locus_tag="VFL49_03780"
     CDS             886..2136
                     /locus_tag="VFL49_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7911124.1"
                     /translation="MLDRMSTDRGLSEEQRMMRQSCRDFVDDVVLPFIKQNWQREWSM
                     VPEDRLPSKILEEAEKIGIRTLGVPEEFGGVELEKGTEVKTFAIVAEELARGDSGLAD
                     KMVQNWKVSVLLRQFAPRHLQEKWFKRLVAEPRFLMAHCLTEPRGASDRWLPYNVPEA
                     AMQTRAVKTDGGWVINGRKQFISNGYDASLYVVYANTNPKVGMLQGTSSFLVPRETKG
                     FSVARCNETLGCRFMNNGEMVFEDMFVPDDHLLVQDSAMGSAGVYFRPGKIIQAAKNL
                     GVGVRAFEATAEYAQNYVQGGRILIKHQAVALRLADMATRIEAVRALLDRASQAVDEN
                     APDAEILVNMAKMFASEEIMKVAQHAVELHGGNGVMLDFGIEKVLRDAVVFLHMDATV
                     DISKFKIIKSMFPETAGKYAGPEN"
     gene            complement(2306..3169)
                     /locus_tag="VFL49_03785"
     CDS             complement(2306..3169)
                     /locus_tag="VFL49_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7911125.1"
                     /translation="MIIGIVCGTLAALAWAMGFVAAKHGIQIGFSPADIAFHRFFWTG
                     LLVLPLILRDGLRDLGGIGWSRASVMTVLSGPPQSLLAYSGFILVPLGHGTTIQPACA
                     ALSGLVLASLVLHEKPTIQRVLGGTIIIAGLLMFGAESLATIGNSGVGGDLLFATAGL
                     FWATFGTLLRLWNVAGTRAVTAVGAVSVIVLAPIYLFIYGASGLAKQSLFENLLQAVV
                     QGGIAGSLPIYLFAHAVIALGGGRAATFPALVPVFGVVIGYLALGVVPSVAQFIGMLI
                     VLVGFHFALRR"
     gene            3316..>3409
                     /locus_tag="VFL49_03790"
     CDS             3316..>3409
                     /locus_tag="VFL49_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET7911126.1"
                     /translation="MAKSRKVIITCAVTGSIHTPSMSPHLPVTAQ"
BASE COUNT          739 a          997 c         1043 g          630 t
ORIGIN      
        1 ggtgatcggt attggcttcc aacacacacg tcgggatttt atggcagttc tgacgatgca
       61 gaacgtgaag caatgcaagt cgctccttgg ctcaaattcg tgctgagtta accgatgtcc
      121 gtttctggcc cttcgcgtca ctttgatgta tcgcaaaatt tggtcgccac tggatagaac
      181 cggacgttga gccggccgga cgcggcgccc ctagtcggag acaatcccta tggctgctct
      241 cgccgctttg aagcgtgcga tgctgtcggc cggccaatga tcggcgggcc tgtagaattc
      301 tgcgaccgcg ggaacgccca gcggcaggac cacccaaggc tgcttggtgg acgtgtagat
      361 gtgaatgtca ggcggaacgc gtgagggatc gtcgagcgtt cccacgcgta tgaaatgaat
      421 ggccggaccg acctgaggat agttgctcca cacggtcacg ccgcagccgg cacagcgcca
      481 gaaaacctga cctttgccgc tggcgctggg cagcgtggtc cgagtgagct cgccttgcag
      541 cagttcgact tgcgacgcct cgataaaggc gttgacggcg aaagccgatc cgctctcgcg
      601 ctggcaccac gtgcaatggc agcaatgcac aaataacggt cgcgccctga ggcggtaacg
      661 gaccgcgccg caagtgcaac cgccctccat ctcattgccc tgccgttcag tcattgcaaa
      721 gctccccgat gtcgaagcaa acgtgaccca tgcgccactg catgatagtc agctttccca
      781 ttgtcaaaag cgccgggtcg gcgccgtccg gcgcggggtg ctctcgactt gacgttacat
      841 ccgggcaaca ctggccggta agaatactca agcgaggaaa tgccgatgct tgaccgcatg
      901 tcgaccgacc ggggtctgtc cgaggagcaa cgaatgatgc ggcagagctg ccgcgatttc
      961 gttgatgatg tcgttcttcc gttcatcaaa cagaactggc agcgcgaatg gtcgatggtg
     1021 cctgaagacc gtttgccgtc aaagattctc gaggaagcgg agaaaatcgg tattcgcacc
     1081 ctgggcgtgc cggaggagtt cggcggcgtc gagctggaga aggggaccga ggtcaagact
     1141 tttgccatcg tcgccgaaga gcttgcgcgt ggcgactcag gccttgccga caagatggtg
     1201 cagaactgga aagtctccgt gctgctgcgg cagtttgcgc cgcgccacct gcaggaaaaa
     1261 tggttcaagc gccttgtcgc cgagccgcgg ttcctgatgg cgcattgcct caccgagccg
     1321 cgcggggcgt cggatcgttg gctgccctac aacgtgccgg aagccgcgat gcagaccagg
     1381 gccgtcaaga ctgacggggg ctgggtcatc aacggccgca agcaattcat ctcgaacggc
     1441 tacgacgcca gcctctacgt cgtttatgcc aacaccaacc cgaaagtcgg catgctgcag
     1501 ggtacctcat ctttccttgt gccgcgcgag accaagggtt tttcggtggc acgctgcaat
     1561 gagacactcg gctgccgttt catgaacaac ggcgagatgg ttttcgagga catgtttgtg
     1621 cccgacgatc atttgctggt gcaggacagc gcgatgggct ctgccggggt ctatttccgt
     1681 ccgggcaaga tcatccaggc ggcaaagaat ctcggcgtcg gcgtgcgcgc attcgaggcc
     1741 acggccgaat atgcccagaa ctacgtccag ggcggccgca tcctgatcaa gcatcaggcg
     1801 gtggcgctgc gtctggccga tatggctacg cggatcgagg cggttcgcgc attgctcgat
     1861 cgcgcctcgc aagcggtcga cgagaatgcg ccggatgccg agattctcgt caatatggcc
     1921 aagatgttcg cgtcggagga aatcatgaag gtcgcccagc atgccgtcga gcttcacggc
     1981 ggcaacggcg tcatgctcga tttcggcatc gagaaagtgc tgcgcgatgc cgtggttttc
     2041 ctgcacatgg acgccaccgt cgacatttcc aaattcaaga tcatcaagag catgttcccg
     2101 gagacggccg gaaaatacgc cgggccggaa aattagcaaa aatttattga ggttcttgtt
     2161 catcgtcatg gccgggcttg tcccggccat ctacgtcttt gacgcgcgtg agaatttaag
     2221 acgtggatgc ccgcgacaag cgcgggcatg acgattctat ttgaggcgat cccgctgtag
     2281 taagcctgac cacctggcgt tgctatcagc gccgaagcgc gaagtggaag ccgaccagca
     2341 cgatcagcat gccgatgaat tgcgcgacac tcggcaccac gccgagggca aggtagccga
     2401 taacgacgcc gaaaaccggc accaaagccg ggaaagtcgc cgcacgtccg ccgccaagcg
     2461 cgatcacggc atgggcgaac agataaatgg gaagcgatcc ggctatcccg ccctgaacga
     2521 cagcctgcag gagattttcg aacaggcttt gcttcgccaa gccgcttgcg ccgtaaatga
     2581 aaagataaat cggtgcgaga acgatgaccg acacagcgcc gaccgccgtt acggcccgcg
     2641 tgcctgcaac gttccagagc cgaagcaaag tgccgaatgt tgcccagaac agtcccgcgg
     2701 tggcgaacaa gagatcgccg ccgacgccgg aattcccgat cgtcgcgagc gactcggcgc
     2761 cgaacatcaa aaggccggcg atgatgattg ttccgccgag gacgcgctga atggttggtt
     2821 tttcgtggag aaccagcgaa gccagaacga gccccgacag cgcggcgcag gccggttgaa
     2881 ttgtggtgcc atgcccgaga ggaacgagaa tgaagccgct ataggcaagc aaagactgtg
     2941 gcggtcccga caggaccgtc atcaccgatg cgcggctcca gccaatgccg ccaaggtcgc
     3001 gcaatccgtc gcgcaggatc agcggcagca ccagaaggcc ggtccagaag aagcgatgaa
     3061 acgcaatatc ggccggcgaa aagccgatct ggatgccgtg ctttgcggcg acaaagccca
     3121 tcgcccacgc cagcgcggcg agcgtaccgc acacgatgcc gatgatcatt gtcggcctat
     3181 gcactttgtc ggatgcggcc atgcagcgca gcgttcaggg caagcggggt gacttcaata
     3241 cggctgatac tctaggattg gccgcgaagt ccatttgcca atcgaaaaca ctcacttcat
     3301 ccggatggaa agctcatggc gaagtcgcgt aaagtcatca ttacctgcgc tgttaccggt
     3361 tcaattcaca ctccgtcgat gtcgccccac cttccggtga cggcccagg
//
LOCUS       DASSRL010000179         7211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1737126, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000179 DASSRL010000000
VERSION     DASSRL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737126"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..574
                     /locus_tag="VFL49_03795"
     CDS             377..574
                     /locus_tag="VFL49_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911127.1"
                     /translation="MQTKFVSLSLIVICAVLALTAWATSVGSSGQAEAITGAGQTISV
                     MELQQGIDTKSLPIQQIVDPI"
     gene            771..944
                     /locus_tag="VFL49_03800"
                     /pseudo
     CDS             771..944
                     /locus_tag="VFL49_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1670..2350)
                     /locus_tag="VFL49_03805"
     CDS             complement(1670..2350)
                     /locus_tag="VFL49_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HET7911128.1"
                     /translation="MMVHVPNVLTPDQVARCREVMEKAAWVDGRVTAGHQSSKVKHNL
                     QLPEGSPEARELGGMVVEALARNNLFMSAVLPKQVFPPLFNRYDAGMTFGAHVDNAIR
                     GIANSGIRIRTDVSSTLFISSPEDYDGGELVVEDTYGSHNVKLPAGDMIVYPATSLHH
                     VMPITRGSRIASFFWTQSMIRHESKRSLLFDMDMAIIKLSRDHPEHTSVVELTSIYHN
                     LLRQWAEL"
     gene            complement(2372..4594)
                     /locus_tag="VFL49_03810"
     CDS             complement(2372..4594)
                     /locus_tag="VFL49_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7911129.1"
                     /translation="MAASTASAQEGGSSGLQLPTIDVSGNNQGGYQATQQSITRLQTP
                     LLDTPQTVNVVPQQEIQERRLTTMEDALRTVPGITFSAGEGGQQGDSPIIRGFVARGD
                     IFRDGFRDPGWYTRDLFDVDRVEVYKGPSSFAFGRGSTGGAINNVSKLPTGSTFVEGT
                     GTITSVGGYRADVDASGKRDNISGRIAALYTDAETPTRDNVWTRRWGVAPSVSAQIDE
                     NTKATLWYIYQGEEGAPDYGWPYLPQPGYRPTTGALVNPGYNGDGSPVTPVPIPRNTW
                     FGIATGPLRDIVTTETHILTARIERELGSNFKITNGTRYLLNDRFGRNTAPRGLANAA
                     MQPFTSGLTTGAGGNGIGYPVDLMTIARERRERETDGTYAVNATDLTGKFDTGIINHT
                     LTAGIELSRETRNQTRTDLCVASNPACYTSLVNPNAGGVPATGFTVIPGNNTLSTDYA
                     AYVIDQMKITRYFELLGSFRFDRFSTHYVDASAGAGMNDLRRTDNLPSYRFGAVYHPT
                     PNSSLYFVYGNSYNPAAELGTLSGNPNNAASVTLAPEKNISYEAGVKVDVLDGGLSLT
                     GAIFRIEKTNLRIPNDPTLPTAQQFLVLDGLARVDGFEIGAAGKLTDKWQVIGGYSYL
                     DSSIEKTSNLAELGHWLPNAPRHNLSLWTTYDVTPKFTVGAGTTYQSDTFVNTTNTTF
                     VPEYWKFDAMVSYKVDNKSTIQLNVYNITDEFYYWQYYQGHAVPASGRWASLSYRVRW
                     "
     gene            4995..6152
                     /locus_tag="VFL49_03815"
     CDS             4995..6152
                     /locus_tag="VFL49_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HET7911130.1"
                     /translation="MADTHAAQLRRLWLNVHLWIALGLCALLVPISISGGLLVWHDEI
                     DAWINPQRYAISGSQALLPPVVYLAQAQEAVVSDPAKPRATALRYPEPGWPMRVVMRA
                     QPSEAGARPRVLTVYLDPSTARVLDVVDFRSSFFGFLHVFHENLTIPEYSGRQIVGWA
                     GVGMLVLSLTGLWLWWPRGGFVRGLRWRRSPLFTFNLHSLLGFWISLPLAVVSATGIY
                     LSFPQTARQAMSSIAPMTPQGQRPGFGQIAQHTVLTADQALAAALASEPSGKPVALFL
                     PVQQRERPLTWRTQISRAGSADVITLMTDDRTGQTSLGAAPLSGDRAAAWIRWIHEGS
                     HSGTVWAVAVTLTGIFPTIFAVTGIIMWLRKRAGRKELGRKGAKPRLRPAE"
     gene            6198..7154
                     /gene="exbB"
                     /locus_tag="VFL49_03820"
     CDS             6198..7154
                     /gene="exbB"
                     /locus_tag="VFL49_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HET7911131.1"
                     /translation="MTFDRHKHHAVMAAIVAVVLVLPAPVIAQTAPAATTPVPQAAPA
                     PSTPSSVAPAATAPATNPPAVEPPATGPATNSPDIALKLPRDLSPWGMFMAADIVVKA
                     VMVGLAFASVLTWTIWFAKAIELMFARRQMRTAIVKLGHAKSWSEANVRAKGRLGGAG
                     ELLRAADTELRMSADALSPTGVKERIASRLERIEAAAGRQMIRGTGILATIGATAPFV
                     GLFGTVWGIMNSFIGISKSHTTNLAVVAPGIAEALLATALGLVAAIPAVVMYNMFSRW
                     IAGYRALHADAAAEILRIVSRDLDRGSFDERPADRRRAIAAE"
BASE COUNT         1399 a         2156 c         2181 g         1475 t
ORIGIN      
        1 taacgcggac tgcagtggcc gcaagggcgg ggctccagca gatactgggg gacgaaggct
       61 gatcataagg cttaaagacc agccatgccg gccgtttatt ccagacaatt ttcgatatta
      121 tagcaaggcg ctatcacctc ctcagcgccg gactcccgca actgcatact gaaaagctat
      181 cgaccgtttc tttcggcacg ccgaccggca aacacaaaaa aaaattggcc ggcgggaata
      241 aatttgcacg gtcgcagagt cgtcaacaac caaacgtcgt cgattgtcgg ctcgcgtctc
      301 atgcttaggc gcccgacggc ggcaattaca tgggcttaat tcactttgca tttgaacttc
      361 tcacgggaga tcggacatgc aaaccaaatt tgtgagtctc agcctaatcg ttatttgtgc
      421 ggtcctggca ttgacggctt gggcaacatc ggtcggctca tctggtcagg ccgaagcaat
      481 cactggagcc gggcaaacca tctccgtaat ggagttgcag caaggcatcg acactaagag
      541 cctgcctatt caacagatcg ttgatccgat ctgaactgtg aaggcctagt cgtgccgtca
      601 cgccatcaaa atcaaaacac cgagagctca ctggtcccta tgatttggct tgccttctac
      661 gcaattgcag tcgctggcgc gttggcttgg ccaaggcatg cgcttgaaac gcgcgaggtc
      721 gccaaagtcg acgacggtgc tcgtgacgct gcggtacaag ggaagaccgt atggaaatag
      781 ctcagtgccg ctatttcctt gcactctgcc aggaaggtaa cttcacccgt gcggccaaac
      841 gctgcggggt ggcgcagccg tcgctaacgc gggcgatcag gaaactagaa agggagttgg
      901 gtgagccgct gttcgtgcgg cgattcgacc gcaccaagct aacgccgttc ggctggaaag
      961 tgtttcccta ttttgatgca atcgcacgca acattgcgga gattaaactt cggtcgcagg
     1021 gagtctctgc cgctgaccgt aaaccgacga aaaaaagcgt gcgcatgctc gatgtagcgt
     1081 catgggcgga ggccgtcccc tcgacgaagc ccttagccgt ggcatcaaac tctgctgacc
     1141 atcaagaagt ttcgatcaac gaataagagg ttcaagtctg cttttgtccc ttcatcgcat
     1201 tgtcgcaact ataaatctta agagttatcg gtaactgtta gcatgaattc caattaggcc
     1261 gcgttgatgc cgtccaggat gatagttcgg cgtctgcgga ccatgcacaa aacgccgaaa
     1321 gacatcgctc gctccctgcg ggcgtttaag agagcgcaca agcttgggct gttgcatcac
     1381 gttagcatcg tgcctggagc ggcggcttgc gaagtcgccc gatcgcagat gggtgtctct
     1441 tatatttgca atgctgtgcc gagattgccg ctcgcccagt gcacgcaaaa ggggtgcctg
     1501 tgtgactatg cgccgattgg gaaggaaccg gttggccggc gaagcaaaag gtcccagctt
     1561 tagggtccag cacgtagata tgtcggcttt tggccgccga gcgaagcgga cattctgcga
     1621 ggtgcgctga caaaacgcgg gttatgagta cgtgccctag tctctcgtcc tacagctcag
     1681 cccactgccg cagcaaatta tgatagatgg acgtcaactc gacgaccgac gtgtgctcgg
     1741 gatgatcgcg cgacagtttg atgattgcca tatccatatc gaacagaagc gatcgtttgc
     1801 tctcgtggcg tatcatgctt tgcgtccaga agaatgacgc gatgcgcgac ccgcgcgtga
     1861 tcggcataac atggtgcagg gaagtcgccg gatagacgat catgtcgccc gcaggcagct
     1921 tcacattatg gctgccataa gtgtcttcga cgaccagttc gccgccgtca tagtcctcgg
     1981 gagatgagat aaacaaggtc gacgacacgt cggtgcggat gcggatgccg ctgttggcga
     2041 tgccgcggat ggcattgtcc acgtgggcgc cgaaggtcat gcctgcgtcg tatctgttga
     2101 acagcggcgg gaacacctgc ttcggcagca ccgcggacat gaacaggttg ttccgggcca
     2161 aggcctcaac gaccatgccg ccaagctcac gcgcttccgg cgagccttcc ggaagctgca
     2221 gattgtgctt caccttggac gactggtgtc ccgcggtgac gcggccgtcg acccacgccg
     2281 ccttttccat cacctcacgg caacgcgcga cctgatccgg cgtcagcacg ttcggcacat
     2341 ggaccatcat cgccggcgcg tcctctctcg cctaccaacg cacacgataa gagagcgacg
     2401 cccagcggcc ggatgccggc accgcatggc cctggtaata ctgccagtaa tagaactcgt
     2461 cggtgatgtt gtagacgttg agctggatgg tcgatttgtt gtcgaccttg tagctcacca
     2521 tggcgtcgaa cttccagtat tcgggcacaa aagtagtgtt cgtcgtgttg acgaatgtat
     2581 ccgattgata ggtcgtgccg gcgccgacgg taaatttcgg cgtgacatcg taggtcgtcc
     2641 agagcgacag attgtggcgc ggcgcattcg gcagccaatg gccgagctcc gcgaggttcg
     2701 acgtcttttc aatactggaa tccaggtagc tgtagccgcc gatcacctgc catttgtcgg
     2761 tcagcttgcc ggcagcgcca atttcgaagc cgtccacgcg cgcgagaccg tcgagcacca
     2821 ggaattgctg cgccgtcggc agagtcggat cgttgggtat gcgcaggttg gtcttttcga
     2881 tgcggaaaat tgcgccggta agactcaggc cgccgtcgag cacatccact ttgactccgg
     2941 cctcgtagga aatatttttt tccggcgcca gcgtcacgct tgcggcatta tttggattgc
     3001 cggacaaggt gccgagttcg gcagccggat tgtacgaatt gccgtaaacg aaatagaggc
     3061 tcgaattcgg cgtcggatga tagaccgcgc cgaaacggta gctcggcagg ttatcggtgc
     3121 gccgcaaatc attcatgccg gcgcctgcac ttgcgtccac gtagtgcgtc gagaaccggt
     3181 cgaagcggaa cgagccgagc agctcgaagt acctggtgat cttcatctgg tcgatcacgt
     3241 aggccgcgta atcggtcgac aacgtattgt ttccggggat caccgtgaag cccgtggccg
     3301 ggacgccacc cgcgttggga ttcaccaggc tggtatagca ggcgggattg gacgccacgc
     3361 acagatcggt acgcgtctgg ttccgcgttt cacgggacag ctcgatgccg gcggtcagcg
     3421 tgtgattgat gatgccggta tcgaacttgc cggtcaggtc ggtcgcattg acggcataag
     3481 tgccatcggt ttcgcgctcg cggcgctcgc gggcgatggt catgagatcg accggatagc
     3541 cgatgccgtt gccccccgcg ccggtggtca aaccgctggt gaatggttgc atcgccgcat
     3601 tggcaagccc gcgcggcgcc gtgttgcggc caaaacgatc attcagcagg taacgcgtgc
     3661 cgttggtgat cttgaaattg cttccgagct cccgctcgat cctggcggtc agaatgtgcg
     3721 tctcggtggt gacgatgtcg cgcaacgggc cggtggcaat gccgaaccag gtgttgcgcg
     3781 gaatcggcac cggcgtcacc ggcgacccgt cgccattgta gccgggattg accagggcgc
     3841 ccgtcgttgg ccggtagccc ggctgcggca ggtagggcca tccatagtcc ggcgcgccct
     3901 cttcgccctg atagatgtac cagagcgtgg ccttggtgtt ttcgtcgatc tgcgcactga
     3961 ccgaaggcgc aacgccccaa cgcctggtcc agacattgtc gcgcgtcggc gtctcggcat
     4021 cggtgtaaag tgcggcgatg cggcccgaaa tattgtcgcg cttgccgctg gcgtcgacgt
     4081 cggcgcgata gccgcccact gacgtaatcg ttccggttcc ctcgacaaag gtcgagccgg
     4141 tcggaagctt cgagacgttg ttgatcgcgc cgccggttga gccacggccg aaggcgaacg
     4201 aggaaggccc cttgtagacc tcgacccgat cgacatcgaa aaggtcacgc gtgtaccagc
     4261 cgggatcgcg gaatccgtcg cggaagatat cgccgcgggc aacgaagccg cggatgatcg
     4321 gactgtcgcc ctgctggccg ccctcgccgg cgctgaacgt gatgcccggg acggtgcgca
     4381 gcgcatcttc catcgtggtg agacgacgct cctggatttc ctgttgcgga accacgttga
     4441 cggtctgcgg cgtgtcgagc agcggcgtct gcaggcgcgt gatggactgc tgggttgcct
     4501 ggtagccacc ctgattgttg cccgacacgt cgatggtcgg cagttgcagt cccgaacttc
     4561 cgccctcctg cgccgacgcc gtcgaggccg ccatcaagga ggcaagactt acggccgtgc
     4621 gcaacaaagt acggttggaa gtttccggag cgcggcccgc cgtcgacttt acatagtgtg
     4681 aagagttgtc gtgtcggtcg aacggctccg accgaatact tgaaatcgcg tagttcatct
     4741 tccccagccc tgatcgaata ctatacttta gaaccgtaat aatcaggctg attattaggc
     4801 tatatttaaa accacggacc gtattcgtgg tcaagaatag actgataatc cacaactatt
     4861 cttgaattgg aatatttcta aactgatgat tactgttttg aatacttaaa aactggacac
     4921 cgatccgcat tttgtatttc acgagcatcc aggggctgat atagctccgc caactaattg
     4981 cgaaaggcgc cgacatggcg gacacgcacg cggcacaatt gcgccgcctc tggctcaacg
     5041 tgcatctctg gatcgcgctc gggctctgcg ccctgctcgt tcccatttcc atctccggcg
     5101 gcctgctcgt ttggcatgac gaaatcgacg cctggatcaa tccgcagcgc tacgcaatct
     5161 cgggttcgca agcgctgctg ccgcccgtgg tctacctggc gcaggcacaa gaagcagtcg
     5221 tcagcgatcc ggcaaagccg cgcgcaacag cactgcgata tccggagcct gggtggccca
     5281 tgcgcgtggt tatgcgcgca cagccgagcg aagccggtgc gcggccgagg gtgctgacgg
     5341 tctatctcga tccgtcaaca gccagggtcc tcgacgtcgt ggatttccgc tcatcgtttt
     5401 tcggcttcct gcacgtattt cacgagaacc tcacgatccc ggaatacagc ggccgccaga
     5461 ttgtcggctg ggccggtgtc ggaatgctgg tcctttcatt gacgggactc tggctctggt
     5521 ggccgcgtgg cggctttgtg cgcgggctgc gctggcggcg atcgccgctt ttcacattca
     5581 acctgcatag ccttctcggc ttctggattt cattgccgct ggccgtggtt tcggcgaccg
     5641 gaatctacct ctcgttcccg cagacggcgc gccaggcgat gtcgtcgatc gcaccgatga
     5701 caccacaggg acaacgcccg ggctttggac agattgcgca gcacaccgtt ctgaccgcgg
     5761 atcaggcgct tgcggcggca ttggcatccg agccgagcgg gaaaccggtg gcgcttttcc
     5821 tgcccgttca gcagcgcgaa cgcccgctga cctggcgcac gcaaatttct cgcgccggca
     5881 gcgcagacgt catcaccctg atgacggacg atcgcaccgg ccagacatcc ctgggcgcgg
     5941 cgccgctctc gggcgaccgt gccgccgcct ggatccgatg gatccacgag ggcagccaca
     6001 gcggaactgt ctgggctgtc gccgtcacct tgaccggaat ttttccaacc atcttcgcgg
     6061 taaccggcat catcatgtgg ctgcgcaagc gcgccggccg caaggaattg gggcgtaaag
     6121 gcgccaaacc gcgtttacgt cccgccgagt gacctcgttg tgctagagtt tggaaggcag
     6181 aaaggatcga gcgttcaatg acctttgatc gtcacaagca tcacgccgtc atggcggcca
     6241 ttgttgccgt cgtcctcgtt ctgccggcac ccgttatcgc acagacggcg cctgcggcga
     6301 ccacgcccgt tccgcaagcc gcgccggcac cttcgacgcc gagttccgtc gcgccggcgg
     6361 ccactgctcc agccaccaat cctccagcgg tcgagccacc cgcaaccggt ccggcaacaa
     6421 attcgccgga catcgcgctc aagctgccgc gcgacctctc gccatggggc atgttcatgg
     6481 cggctgatat cgtcgtgaaa gcggtgatgg tcggcctcgc atttgcgtcc gtgctcacct
     6541 ggacgatctg gttcgccaaa gccatcgaat tgatgttcgc gcgacgccag atgcggacgg
     6601 cgatcgtgaa gctgggccat gccaagtcgt ggagcgaagc caatgtccgg gcgaagggcc
     6661 gcttgggtgg cgccggcgag ttgcttcgcg ccgccgacac agagttgcgg atgtccgccg
     6721 atgcgctttc gccgaccggc gtcaaggagc gcatcgcctc acgcctggag cgcatcgaag
     6781 ccgcggccgg ccggcagatg attcgcggca ccggcattct ggcaaccatc ggcgccaccg
     6841 cgcctttcgt cggcctgttc ggcacggtat ggggcatcat gaacagcttc atcggcattt
     6901 cgaagtcgca caccaccaac cttgcggtcg tggcgcccgg cattgccgag gcactgctcg
     6961 cgaccgcgct cggcctcgtc gcggccattc ccgcggtggt catgtacaat atgttttcac
     7021 gctggatcgc cggctaccgc gcgctgcatg cggacgccgc cgccgagatt ctccgcatcg
     7081 tcagccgcga cctggaccgc ggaagcttcg atgaacggcc cgccgatcgc cgacgtgcca
     7141 tagccgcgga gtgatgtcgt gagcgtacgt cttgaccacg gcgacgagca gctcggcgaa
     7201 gtccacgaga t
//
LOCUS       DASSRL010000180         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_180058, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000180 DASSRL010000000
VERSION     DASSRL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180058"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFL49_03825"
     CDS             <1..1062
                     /locus_tag="VFL49_03825"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7911132.1"
                     /translation="VSLWFGQNEECGLRCPYHGWKYDVTGQCVDLPSEPEESGFRKNI
                     KLKSYPCIEKAGIVWAYMGPPALQPPPPALEWTDVEPPQRFVSKRLQECNYLQAMEGG
                     IDSSHVSWLHGSELNKDPLFKGSKGNVYNEQDRMPLFDVEEFPGGLLIGARRNAEGGK
                     YYWRITPWIMPWYSIIPPRAGHPLGAHAWVPIDDENCWAWSINYHPKRALTKAEVSAM
                     QDGEGIHVKYVPGTFIPLANKSNNYLLDREAQRHGHSSTGVDGIAMQDASIQESMGPI
                     QDRTREHLCATDSGIIMTRKILLRAAKAAADGGVVPATEPEAQRVRSASIELDKDVSF
                     TQGARHGLYAAIGTDPLSV"
     gene            complement(1072..2190)
                     /locus_tag="VFL49_03830"
     CDS             complement(1072..2190)
                     /locus_tag="VFL49_03830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7911133.1"
                     /translation="MRLGYFTMPVHPLGRDWSQTLREDREAIILADKLGFYDAFVGEH
                     LTDACENITNSMMFQATLIHDTKTIKLATGTANLAQMHPVVIAVNAAMFDHLSQGRFV
                     MGVSAGALTSDSEAIGILDLDRNKIFAEAIDVILAVWERNPPYDIDFPDNRFKVGFAR
                     TAALELGVGILAKPFQKPRPEIVGTVVAPFSPGVVLMGKRDFHPLSANFLLPKHLKSH
                     WTNYAKGKAEVGHKANVGDWRVARTIFVADDDKTALRYGLDDARSPYRHYFGNMRAKM
                     IRGNRLYVFKSHKEQPDSEITLDYVMDNCVFCGTVNKVVDQILAMHEETGDFGELVYA
                     GMDWADPGLARRSMQLMAEEVMPRVNAAIGKIAKTAAE"
BASE COUNT          463 a          718 c          737 g          474 t
ORIGIN      
        1 gtctcgctct ggttcgggca gaacgaggaa tgcgggctgc gttgccccta tcatggctgg
       61 aaatacgacg tgaccggaca atgcgtcgat ctcccgtccg agccggagga atcgggcttc
      121 cgcaagaaca tcaagctgaa atcctatccc tgcatcgaga aggccgggat cgtctgggcc
      181 tatatggggc cgcccgcgtt gcaaccgccg ccgccggcgc tggaatggac cgacgtggag
      241 ccgccgcaac gtttcgtctc caagcgcctg caggaatgca attacctgca ggcaatggag
      301 ggcggcatcg actcaagcca tgtctcgtgg ctgcacggca gtgagttgaa caaagatccg
      361 ctgttcaagg gctccaaggg gaatgtctat aacgagcagg accggatgcc gctgttcgac
      421 gtcgaggaat ttcccggcgg cttgctgatc ggcgcgcgtc gcaatgccga aggagggaaa
      481 tattactggc gcatcacgcc ctggatcatg ccctggtatt cgatcattcc gccgcgcgcc
      541 ggccacccct tgggcgcgca cgcctgggtg ccgatcgacg atgagaactg ctgggcctgg
      601 agcatcaact atcatcccaa gcgcgcgctg acgaaggcgg aagtttcggc gatgcaggac
      661 ggtgagggca tccacgtcaa atacgttccg ggcactttta ttccgctggc caacaagtcg
      721 aacaactatc tgttggatcg tgaggcgcaa aggcacggcc attcgtcgac cggcgtcgat
      781 ggcatcgcca tgcaggatgc atcgatccag gaaagcatgg gcccgatcca ggaccgcacg
      841 agggagcatc tgtgcgcgac tgacagcggc atcatcatga cgcggaagat tttgctgcgc
      901 gcggccaaag cggcggccga cggcggcgtc gtgccggcga ccgagcccga ggcgcaacgg
      961 gtgcggtcgg catcgatcga gctcgacaag gatgtttcgt ttacgcaggg cgccaggcac
     1021 ggcctttacg ccgcgatcgg aacggatccg ctgtccgttt gatcctggag cttattccgc
     1081 cgccgtcttc gcaattttgc ctatggcggc gttcacgcgc ggcatcactt cctcggccat
     1141 gagctgcatc gagcgcctgg cgaggcccgg gtctgcccag tccattccgg cgtagacgag
     1201 ttcgccgaag tcgccggttt cctcgtgcat cgccagaatt tgatcgacca ccttgttgac
     1261 cgtgccgcag aatacgcaat tgtccatgac ataatcgagc gtgatctctg agtccggctg
     1321 ctccttgtga ctcttgaaca catacagccg gtttcctcgg atcatcttgg cacgcatgtt
     1381 gccgaagtaa tgacgatagg ggctcctggc gtcgtcaaga ccgtaacgca gcgcggtctt
     1441 atcatcatcg gcgacgaaaa tcgtgcgcgc cacgcgccag tcgccgacat tggccttatg
     1501 gcccacctcg gccttgccct tggcatagtt ggtccagtgc gatttcaggt gcttcggtag
     1561 aaggaagttc gccgataaag gatgaaagtc gcgctttccc atcagcacga caccggggga
     1621 aaacggcgcg acgaccgtgc cgacgatctc cggccgcggc ttctgaaaag gcttggcgag
     1681 gattccgacg cccagctcga gcgccgcagt gcgcgcgaaa ccgaccttga agcggttatc
     1741 cggaaagtcg atgtcgtaag gcgggttgcg ctcccacacg gcgagaatga cgtcgatcgc
     1801 ttcggcgaaa atcttgttgc ggtcgaggtc gagaattccg atcgcttcgg aatcggaggt
     1861 gagggcgccc gcgctgacgc ccatcacgaa gcgcccttgc gacagatgat cgaacattgc
     1921 ggcgttcacc gcgatcacca ccgggtgcat ttgcgcgaga ttggcggtgc cggtcgcaag
     1981 cttgatcgtc ttggtgtcgt ggatgagcgt cgcctggaac atcatgctgt tggtgatgtt
     2041 ctcgcacgca tcggtcaggt gctcgccgac aaaagcatcg tagaagccga gcttgtcggc
     2101 gaggatgatc gcctcgcgat cctcccgcag tgtctgcgac cagtcccggc ccagtggatg
     2161 cacgggcatg gtgaagtaac ccaaccgcat ttgcgtctcc gactcccaac tcctgtggtc
     2221 cgcaaactaa ccgaccccgt gcgccgttgc agcaaatctt cggacggccg tgacgcgcag
     2281 tctcgtcgca agtcattgtt ttcacgatgt taaacttggt ccgctcgcga gcattgcctt
     2341 gcattgccgc gtggcagacg atgtttccac aggcgcgcct cgccagattt cg
//
LOCUS       DASSRL010000181         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_75030, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000181 DASSRL010000000
VERSION     DASSRL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75030"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..972
                     /locus_tag="VFL49_03835"
     CDS             214..972
                     /locus_tag="VFL49_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911134.1"
                     /translation="MSQRVLITAGGAGIGREFARAFAAKGAKVFVCDIDEKALAAVAT
                     ETPGLISLRCDMGSRTEIERMVPEAVDALGGLDVLINNAGIAGLTLPIADYPPDDWDK
                     VVAVNLTAMFDVCRLAIPHLKKSKAGCIINMSSVAGRFGFPNRSPYAATKWGVIGLTK
                     TLAMELGEWGIRANAIAPGAVEGDRIVRVFRGRAQISGRSLDEVKADALAAASIKAMV
                     DPKDIAQLAVFLASDAAKAISGQVIPIDNDRQNA"
     gene            1284..1544
                     /locus_tag="VFL49_03840"
     CDS             1284..1544
                     /locus_tag="VFL49_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911135.1"
                     /translation="MKIVTRALFACAVALAAATPALAADSGMDHRMHASDAKRAAPAT
                     DQSVRDHERARRAIDSMAHEPAGPSEDYGYRYFHDFGIGSQS"
     gene            2042..2389
                     /locus_tag="VFL49_03845"
                     /pseudo
     CDS             2042..2389
                     /locus_tag="VFL49_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2508..3257)
                     /locus_tag="VFL49_03850"
     CDS             complement(2508..3257)
                     /locus_tag="VFL49_03850"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7911136.1"
                     /translation="MSDLKGKVTVVTGASKGIGAGIARSLAAAGAAVVVNYASSREGA
                     ERVVEDIKVKGGKAVLIKADVAKTADVKRLFDETKRVFGRLDVLVNNAGVYHFQPLED
                     VTEEEFHRQFNTNVLGTILATQEAAKHFGTEGGSVINIGSTASLDAIPTASVYSATKG
                     AVDTFTRVLAAELGPRKIRVNVIAPGGTETEGLHAAGVIGSDFEKATIARTALGRLGQ
                     PDDIARVAVFLASDDSAWMTGERLAAAGGYH"
BASE COUNT          657 a          995 c          988 g          642 t
ORIGIN      
        1 ggcactgcac ctcaattcac tcgaaccagg gcacctcatt ccaatacaaa cctgctccag
       61 ggccgaaccg cccaggcgag cacatcaaaa accgaatcac tgccggtgac tctatccgat
      121 tcggaccttt caagggtgaa cacagcatcg gcaaaccggt cggctcccgt tgtcccgtcc
      181 cggctggtgt gatctgatcg cgatatcgga atcatgtccc aacgtgtcct gataacggcc
      241 gggggcgccg gcatcggccg cgagtttgcg cgtgccttcg ctgcgaaggg cgccaaggtc
      301 ttcgtttgcg acattgacga gaaggcgctt gctgccgtcg cgacggagac tcccggcctc
      361 atctccttgc gctgcgatat gggcagccgg acggagatcg agcgcatggt gcccgaggcc
      421 gtagacgcgc tcggcgggct cgacgtccta atcaataatg ccggcatcgc aggcctgacg
      481 ctgccgatcg ccgattatcc gcccgacgac tgggacaagg ttgttgcggt caatcttacc
      541 gccatgttcg acgtctgccg cctggccatt ccgcacctga aaaaatcgaa ggcgggctgc
      601 atcatcaaca tgtcgtccgt cgccggccgc tttggtttcc ccaaccgcag cccttatgca
      661 gcaacgaaat ggggggtcat cggcctcacc aaaaccctgg cgatggaact cggcgaatgg
      721 gggattcgtg ccaatgcgat cgcgccgggc gcggtcgagg gcgaccgcat cgtgcgcgtt
      781 tttcggggcc gcgcgcagat cagcggccgg tccctcgacg aggtgaaagc tgacgctttg
      841 gccgccgcct cgatcaaggc aatggtggac ccgaaagaca tcgcccagct ggcggtgttt
      901 ctcgcttccg atgctgcgaa agcgatctcg ggccaggtga tcccgatcga taacgaccgg
      961 cagaacgctt agagggattg ggccgaacct gcggcaaata cacctcgcag ctttccatca
     1021 acgtatcgca tgacgcgcga ttgctgaccg aaaaagttca acaacaaata acgtgtccgt
     1081 gatggcatgc gtgcggcacg gccagcgcgc acaaggggca tgaccgaggc gtgagtgtgc
     1141 cgtcgttgtg attgttcgct cccttttgtg tgaacggcag tgcaatgccc gtgaccaaat
     1201 cgtggattga gtgtgaaggc gcgctccgcc acacggggtt catgcaagaa atccatcttg
     1261 cctgaacgaa tggacggaaa atcatgaaga tcgtgaccag ggctctcttt gcttgtgctg
     1321 tggcgctggc agcagctacc ccggcgcttg cagccgatag tgggatggat catcggatgc
     1381 acgcttctga cgcaaaacgt gcggctcctg cgacggacca gagcgttcgc gaccacgagc
     1441 gggctcgccg tgcgattgac tcgatggcgc atgagccggc cggaccgtcc gaagactacg
     1501 gctatcgcta tttccacgat ttcggaatcg gcagtcagag ctgagcgtcc ccggcttgga
     1561 tcgaactgcc ggaggggccg ccgcgaggcg gcctctcttg cgttaacgaa ttcggcaacg
     1621 gcgttgcgag atgcaacggg ctcgtgaaaa attctgtcgt ataccgccaa tccgcagacg
     1681 aaattgtcgc ggcgggccgc ctcgtcttaa cgtttaacct tacctggtca gcttaccgga
     1741 cggattcagt agcggctgaa aacgcaagcg ggtacgcgtt catccatcac atgaaaggca
     1801 aagcggaaaa caccggtcac acggccggac cactttggaa ggatggagta cagcgttgaa
     1861 aaaaatagcg atcttgattg cagcagtttt ttcaactgcg ctcgttgtga acgccagtgc
     1921 gacgcctcgg cagaagcagc cgcgtcagac gatcacctgt gacctgcggg gctgttctga
     1981 tcatgtccct caggcagcac gtgcagcaaa cgcttatgcg aatgcaccgg ttgacgccaa
     2041 cggtggcgca gttgtgagcc ggaggccgca aggttgcccg cgtgcattct gcggctgcga
     2101 ggcttcgctt tatttattcg gaaagatccg gcctgaactc aatctggccg caaactggat
     2161 ccggaaattt ccccgtacat cacccgcacc cggcatggcc gccgcgcgga ggggccatgt
     2221 catggtgctc atgagtcacg ttgagggcaa cgactggctt gttcacgacg gcaattccgg
     2281 gggcgggtta acccgcgagc acgtcaggtc gatcaggggc tatgtgatcg tcaacccacg
     2341 cggcgcctac gctgaaaatc cgcccggggg caattcgaac gtaaattaaa ggcccgaaca
     2401 aaaagaaggg ccggcaatgc gacccttcag aacgcaatcg attgcagggt aggggcgagg
     2461 ccggcaacgg cctgtcaact tccgtcgcgc atgtccgttc cccggtctca gtgatagccg
     2521 cccgcggcag cgagacgctc accggtcatc caggccgaat cgtcggacgc gagaaacacc
     2581 gcaacgcgcg cgatatcatc gggctgcccc aggcggccaa gcgccgtacg cgcgatcgtc
     2641 gccttctcga aatcgctgcc gataacgcct gcggcgtgca ggccttccgt ttcagtgccg
     2701 ccgggagcga ttacattgac gcggatcttg cgcggaccga gttcggcggc gagcacgcgc
     2761 gtgaaggtgt ccaccgcgcc cttggttgcc gaatagaccg atgccgttgg aatggcatcg
     2821 aggctggcgg ttgaaccgat attgataacg ctgccgcctt ccgtcccgaa gtgctttgcc
     2881 gcctcctgtg tcgccaggat ggtgccgaga acattggtat tgaactggcg gtggaattct
     2941 tcctcggtaa cgtcttcgag cggctggaaa tgatagacgc ccgcgttgtt caccagcacg
     3001 tcgagtcggc cgaaaaccct cttcgtttcg tcgaacaggc gcttgacgtc ggctgtcttt
     3061 gcgacgtcgg ccttgattaa gaccgccttg ccacccttga cttttatatc ttcgacaacg
     3121 cgctccgcgc cttccctgct cgacgcgtaa ttgaccacca ctgcggcgcc ggcggcggca
     3181 aggcttctgg caatgccagc gccgatgccc ttcgacgcac ccgtgacgac tgtaacttta
     3241 cctttgaggt cgctcatgga accctcctgc gacattgctt tg
//
LOCUS       DASSRL010000182         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1527157, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000182 DASSRL010000000
VERSION     DASSRL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527157"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFL49_03855"
     CDS             <1..1137
                     /locus_tag="VFL49_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HET7911137.1"
                     /translation="WHVARDILNQEKVNFVVMMLGVSDRQNIRERDLAKEKEADKKKE
                     KEKASKQASEKDAQNKDHGEDAEQDAVAAPETQPGRRANGIIEFRTDQWAEIYSKRID
                     ETIAALKSKGVPVFWVGLPSIRGTKSTADAVYLNDLYRARAERAGVIYIDIWDGFVDE
                     AGKYSNFGPDYEGQMRRLRSSDGVFFTKSGALKLAHYVEREIRRQMNSRVAPIAIPSG
                     PVAPATPDGKPAARPVAGPVVPLTVAPTTSEDLLGGAGNSSPHGDAIATSVLVKGEPV
                     AAPRGRADDFTLKPDSDGKAAVPPAAAVPAGAMASAPAETARPETAAAPDKKAAAPKG
                     APAAKTTQNAPPKHRTEPKPVRPQQRDDVPRPPRPIGLFGGGFR"
     gene            complement(1183..>2038)
                     /locus_tag="VFL49_03860"
     CDS             complement(1183..>2038)
                     /locus_tag="VFL49_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET7911138.1"
                     /translation="FKMEKIHIDRRVEQMRGEGVTFHYNAHVGVNIPADRLVKEHDAV
                     VLTGGSEESRDLPIPGRDLKGIHFAMDFLPQQNRRVSKEPQGNVEPILATGKKVVVIG
                     GGDTGSDCIGTSIRQGAVSVTNFEIMSQPPEHENKMLTWPDWPLKLRISSSLEEGATR
                     EFAVLTQKFTGENGRVNKLHCTHVDAQFKAVPGSEFVIEADLVLLAMGFVHPVHEGML
                     KGLNVALDQRGNVSADTMAYQTSVPKVFAAGDMRRGQSLVVWAIREGRQAAHAVDKFL
                     MGSTTLPR"
BASE COUNT          406 a          649 c          635 g          348 t
ORIGIN      
        1 tggcatgtgg cgcgcgacat tctcaaccag gagaaagtaa atttcgtcgt gatgatgctc
       61 ggggtgagcg atcgccagaa tatccgcgag cgcgacctgg caaaggagaa agaggccgac
      121 aagaagaaag aaaaagaaaa agcaagcaag caagcatcgg aaaaagatgc gcagaataaa
      181 gatcacggtg aggacgcgga gcaggatgct gtcgcggctc ccgagaccca gccgggcagg
      241 agggcaaacg gcatcatcga gttccgcacc gatcaatggg ccgagattta ctcgaagcgc
      301 atcgacgaga cgatcgccgc gctcaagagc aaaggcgtgc cggtgttttg ggttggcctg
      361 ccatcgatcc gcggcacgaa gtcgaccgcc gatgccgtct acctcaatga cctctaccgt
      421 gctcgtgcgg agcgcgccgg cgtgatctac atcgacatct gggatggatt cgtcgacgaa
      481 gccggaaaat actccaattt cggtcccgac tatgaagggc agatgcgccg cctgcgctcg
      541 agcgacggcg tcttcttcac aaagagcggt gcgctcaagc tggcgcacta tgtcgagcgc
      601 gaaatccgtc ggcagatgaa cagccgtgtg gcgccgattg ccatcccttc cgggcccgtc
      661 gcgcccgcga cgccggatgg caagccggca gcacgcccgg tggccgggcc tgtagtgccg
      721 ttgacggtcg cgcccacgac gtccgaggac ctgttgggag gcgccggaaa cagctccccg
      781 catggcgacg ccattgcgac cagcgtgctc gtgaaaggcg agcccgttgc cgccccgcgc
      841 ggtcgtgccg acgatttcac cctgaagccc gatagcgacg gcaaagctgc cgtaccgcct
      901 gctgcggcgg tgccggctgg cgccatggcg tccgcgccgg cggagaccgc gcgtcccgag
      961 acggctgccg cacctgacaa aaaagcggcc gcgcccaagg gcgcaccggc tgcaaaaacc
     1021 acacaaaatg ctccgccgaa gcacaggacg gaacccaagc cggttcgccc gcaacagcgc
     1081 gacgacgtac cgcgcccgcc gcggccgatt ggcctgttcg gcggcggctt ccgttgatcc
     1141 acacggtcat tgtcggtcga ggataggcgc gccctgaaag cgtcagcgcg gcagtgtcgt
     1201 cgatcccatc agaaacttat cgacagcatg cgccgcctgc cggccttcac ggatcgccca
     1261 gacgaccaac gattgaccgc gccgcatgtc gccggctgca aagaccttgg gcaccgaggt
     1321 ctggtaggcc atcgtgtcgg ccgatacgtt gccacgctgg tcaagggcga cgttcaagcc
     1381 cttgagcatg ccttcgtgca ccggatgcac gaagcccatg gccagcagca cgagatcggc
     1441 ttcaatcacg aactcgctgc cgggcaccgc cttgaactgc gcgtccacat gcgtgcaatg
     1501 gagcttgttc acccggccgt tctcgccggt gaatttctgt gtcagcactg caaactcgcg
     1561 cgtggcgcct tcctcaaggc ttgacgatat gcgcagcttg agcggccagt ccggccaggt
     1621 gagcatcttg ttctcgtgtt ccggcggctg cgacatgatc tcaaagttag tgaccgacac
     1681 cgcgccctgg cggatcgatg tgccgatgca atccgagccg gtgtcgccgc cgccgatgac
     1741 cacgaccttc ttgcccgtgg caagaatcgg ctcgacgttt ccctgcggct ctttgctcac
     1801 gcgccggttc tgctgcggca gaaaatccat tgcgaaatga atgcctttga gatcgcgccc
     1861 gggaataggc aggtcgcggg actcttcgct gccgccggtg agcacgacgg cgtcatgctc
     1921 tttcaccagt ctatcggccg ggatattcac gccgacatgc gcgttgtaat ggaatgtgac
     1981 gccttcgccc cgcatctgct cgacgcggcg gtcgatgtgg attttctcca tcttgaag
//
LOCUS       DASSRL010000183         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1352169, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000183 DASSRL010000000
VERSION     DASSRL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352169"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFL49_03865"
     CDS             complement(<1..884)
                     /locus_tag="VFL49_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7911139.1"
                     /translation="MHKARLAAAALLALPIAEANAQSDWFTSSPAYQPFPGFYVGMEG
                     GANWNFNHSMGFANAYPTTGWAAGAILGYDFVGPRIELEGVFRQNSTYFTGLGTFGSF
                     NASRDLNQVAVMANLLYDFAPGAVITPYIGAGAGVAFVDGMDIFGTTTSLSNTVFAYQ
                     GIIGVGYNVDSNLRINLDARYHGTTDPASAYGNWNNNNITAMLSVTYKFGQPVAAAPP
                     PAPAAPSAPSFMVFFDWDRSNLSQQALSTIQQAADAYRTRGSARITATGHTDTSGPAS
                     YNMALSLRRANAVKDALVR"
     gene            complement(1426..>3412)
                     /locus_tag="VFL49_03870"
     CDS             complement(1426..>3412)
                     /locus_tag="VFL49_03870"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET7911140.1"
                     /translation="GAASIDRLEPPRQGNRFALLARRLEAMARTAKIIGWSAGILALL
                     LVVAGGATYFFATSDWVRGQVETRAGAYSGRKTTIDDIKIDWGPTAHVRLAGVQVANS
                     SWGKAPYMLKAEEVDFDIRLWPLLRGDFVLPHLTLRKPEVYLERNEKEQTNWDFSQSP
                     VAAGAAKAVAPKERHETPLIGRLEIDGGQLGYSDLKRKIDLKGTISTATGEAGGQPEA
                     KLELAGTLENQPLKVHFVGGSAVMLRQTDKPYPVDLDIAYGATKLAVKGTLQDPIQWK
                     GADVQIELAGPNLADIYPLLGIPGPRTSPYRITGRLEREPGVWKVTNSTWHVGDSDLA
                     GDIEIDERQTPSHLIARLASRHLTFADLAPLIGASPGKSGNVSREQAATKRQLQAQGE
                     LFPNVPLHVERLRAMNMDVSLDAKRVLAPSYLPITALAFAVHVEDGVATVNPLKLAVA
                     GGTVDGELKVDARTDIPAVRTALALRNLDFGAFFRGSRFFDTTKGKIQGRMSLAGTGR
                     SLAQVMGTSNGYIDLAMQGGSVSDLMVSLAGLQIVDALVLYVTGDHRIPIRCALGRLA
                     FDHGTVTFQKTVLDTQKSVLHVEGQAELKSQVVNVTVTADPKKFDLLDLHGPVTVRGK
                     IRKPVIAVKVPIPHPVIGDAKDVACEALTEQLLSGRP"
BASE COUNT          640 a         1058 c         1059 g          655 t
ORIGIN      
        1 cgcaccaaag catccttgac ggcattggca cggcgcagcg acagcgccat gttgtagctc
       61 gccggtcccg acgtgtcagt atggccggtc gcggtgatgc gggcgctgcc tctggtccgg
      121 taggcatcgg cggcctgctg aattgtactc aaggcctgct gcgataggtt ggaacgatcc
      181 cagtcgaaga agaccatgaa cgatggcgca ctcggggctg ccggcgctgg cggcggtgca
      241 gccgctactg gttggccgaa cttgtaagtc acgctcaaca tcgcagtgat gttgttgttg
      301 ttccaatttc cgtacgccga agcgggatct gttgtcccgt gataacgggc gtcaagattg
      361 atgcgcaaat tgctgtcgac attgtagccg acaccgatga tgccttggta ggcaaacacg
      421 gtattgctga gcgaggttgt agtgccgaaa atgtccatgc catcgacgaa cgcgacgccc
      481 gcgccggcac cgatatacgg cgtaatcacc gcgccaggtg cgaagtcgta gagcagattc
      541 gccatgacgg cgacctggtt gagatcgcgg ctcgcattga acgaaccgaa agtcccaagc
      601 ccggtaaaat acgtcgagtt ctgtcggaac acgccttcca actcgattcg cgggccgacg
      661 aagtcataac caagtatggc gcccgcggcc cagcccgtcg tgggataagc gttcgcgaag
      721 cccatgctgt gattgaagtt ccaattcgca ccaccctcca tgccgacata gaaccccgga
      781 aacggctgat aggctggaga cgatgtgaac cagtccgatt gcgcgttggc ctctgcaatg
      841 ggtaatgcaa gaagggctgc cgcggcaagt cgtgctttgt gcatgttccc ctcacgaccg
      901 ttgctgatcc gtgaacggca caagccggcc aaaggttacc catagttgcc acacttcgcc
      961 gtgtttctgt caaactgtgg tattttggca acctttttgg ttccgtccgt ttggaacttt
     1021 cttaccggcc aacagcctac cgatggccag agcagctggc ccggctctgg catcgctttt
     1081 gcccagcaag gctaaggcaa gagaatgaga gcgatgctgc accgctcgac cgggcatctc
     1141 aaggcagcgt cgaacgaatc atgttgctcg acctctcgct gcggttgggc atcatgaatt
     1201 cgtccccggg caccgcgtgc gggcctgcca tgaccccaat atctagttgg ccagcgctcg
     1261 aagacacaag tcactggcag tgatcgcatc cagttcgccc ggccgttctc gatctgatac
     1321 tccgcctcga acacaaaccg catggtgcgg tccgcattcg tcccgccgca gtcgcagata
     1381 cggctcaacg tcgcgtccat acaccggccg gtccgccaac gagcatcagg gcctgccgga
     1441 cagcaattgt tcggtgagcg cttcgcaggc gacgtccttg gcatcgccga tcacgggatg
     1501 cgggatcggg acctttactg cgatgacggg cttgcggatc ttgccgcgca ccgtgaccgg
     1561 cccgtgcagg tccagcagat cgaacttctt ggggtcggcc gtcaccgtga cgttgacgac
     1621 ctgcgatttc agctctgcct ggccctcgac gtgcaggacc gatttctggg tatccagcac
     1681 ggtcttctgg aaggtcaccg tgccatgatc gaaggcaagc cggccgagcg cgcagcggat
     1741 ggggatgcga tggtcacccg tcacatacaa aaccagcgca tcgacgatct gcaggccggc
     1801 gaggctcacc atcaagtcac tgaccgatcc accttgcatg gcaagatcga tgtagccgtt
     1861 cgacgtgccc atgacctggg ccagcgaacg accggtgccc gccagcgaca tccgaccctg
     1921 gatcttgccc ttggtggtgt cgaagaagcg cgagccgcga aagaaggcgc caaagtcgag
     1981 attccggagc gcgagggcgg tacgcacggc cggaatgtcg gtgcgcgcgt ccaccttgag
     2041 ctctccatcg accgtgccac cggccaccgc gagtttgagc ggattgacgg tagccacgcc
     2101 gtcctcgacg tggacagcga aggcgagcgc cgtgatgggc agataggacg gcgccaggac
     2161 ccgcttggca tcgagcgaca catccatgtt cattgcgcgc aaacgctcga catgcagcgg
     2221 cacgttcgga aacagctcgc cctgcgcctg aagttgccgc ttggtcgcgg cctgctcacg
     2281 cgagacgttg ccgcttttgc cgggtgaggc gccgatcagg ggcgcgaggt cggcaaaagt
     2341 cagatgccgg gaagcaagtc gagcaatgag gtgcgacgga gtctggcgct cgtcgatctc
     2401 gatgtcgccc gccagatcgc tatcgccaac atgccaggtc gagttcgtta ctttccacac
     2461 gcccggctcg cgctcgagtc ggccggtgat gcggtagggc gacgtccgcg gtcccggaat
     2521 gccgagcagc ggatagatgt cggcgaggtt cggtccggcc agctcgatct gcacatccgc
     2581 gcccttccac tggatcgggt cctgcaatgt gcccttcacg gcgagcttcg tcgcgccata
     2641 ggcgatgtcg agatcgaccg gatagggttt gtcggtctgc cgcagcatca ccgctgagcc
     2701 gccgacgaaa tgcaccttga gcggctggtt ttcgagcgtg ccggcaagct ccagcttggc
     2761 ctcgggctgg ccaccggctt cccctgtcgc ggtcgagatc gtccctttga gatcgatctt
     2821 gcgcttgaga tcgctgtagc cgagttgccc gccgtcgatc tccagtcgac cgatgagcgg
     2881 cgtttcgtgc cgctctttgg gtgccaccgc cttggccgcc ccggctgcga ccgggctttg
     2941 gctgaaatcc cagttggtct gctccttctc gttgcgctcg agatagacct ccggcttgcg
     3001 cagcgtgaga tgaggaagca cgaagtcgcc gcgcagcagc ggccacagac ggatgtcgaa
     3061 gtcgacctcc tccgccttca acatgtacgg cgctttgccc cagctcgaat tagccacttg
     3121 cacgccagcc agacgcacat gcgcggtggg tccccagtcg atcttgatgt cgtcgatggt
     3181 ggtcttgcgg ccggaatagg cgccggcgcg ggtctcgacc tgaccacgca cccagtcgga
     3241 ggttgcgaag aaataggttg caccgccagc gacgacgagg agcaaggcta ggatgcctgc
     3301 cgaccagccg atgatcttcg cggtgcgcgc catggcctcc aaccgccgtg ccaaaagtgc
     3361 aaaacggttg ccttgtcgag gcggttccag ccggtcaatt gaggcggcac cg
//
LOCUS       DASSRL010000184         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_390052, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000184 DASSRL010000000
VERSION     DASSRL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390052"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFL49_03875"
     CDS             complement(<1..675)
                     /locus_tag="VFL49_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7911141.1"
                     /translation="MTTSAYPKFKVCAAHVAPVFLDAPATVDKACVLIAEASRAGARL
                     IAFPESFVPGFPVWAAVQAPIKSHDFFKRMAANSIEIPGPEVTALCDAARDNAIVVSI
                     GISERSPVSVGCLWNTNVLIDADGNLINHHRKLVPTFYEKLIWANGDGEGLRVSNTNI
                     GKIGMLICGENTNPLARYSLIAQGEQVHISSYPPVWPTRPANEPGRYDLASAIRIRAG
                     AHSFEGK"
     gene            complement(730..2004)
                     /locus_tag="VFL49_03880"
     CDS             complement(730..2004)
                     /locus_tag="VFL49_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911142.1"
                     /translation="MARKLSRRSFLEVALAGGALGTANLTLMRDVYAQASGPIVIGHH
                     CDLTGVISSWGLWHDKAAKAAVDIINQGGGIAGRKVELATEDTESNPASGARKLRNLV
                     QRLNAEFIVGSVHSGVMLAAIPIASELKTIYFSTGEATEATGAKGTRFSFRTGTDTYS
                     IAAASLPWCVENFGKKWTIIYADYAWGQSTNQESKHYIEKSGGKVLNSIPIPLDAKDF
                     VPYLSQIPGESEVLLPAFIGSLSVAFYTQAKNMGLDKKMKMYSSSASIESIAPDDIQG
                     AAEGVYFFENFPRMLKAKDDEFHREFIKRMGIDEVHAREVGGTRVMDKSHGWQSWEDI
                     FAIKQAIEASGYKTRKDVEGAIQALEGIQLKNSLAHPQGDKIIRKEDHVGIIDCYISR
                     VENGKLEVKKRIPKEELAKNMPLRHNLSQMPV"
     gene            complement(2030..>2337)
                     /locus_tag="VFL49_03885"
     CDS             complement(2030..>2337)
                     /locus_tag="VFL49_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7911143.1"
                     /translation="SGGEQQMLAIARCLMMRPKLIILDEPTEGIMPRLVSQIRREIHR
                     INQSGVSILLVEQNVETALKLCPHVFLMEKGTIVHSGTSHELKSQPEIVHRYLGLSH"
BASE COUNT          431 a          694 c          713 g          499 t
ORIGIN      
        1 cttgccctcg aaagaatgcg cgccggcgcg gatgcggatc gccgaggcaa ggtcataacg
       61 gcccggctcg ttcgctggcc gtgtcggcca gaccgggggg taggatgaaa tgtgcacctg
      121 ttcgccctgc gcgatcagcg aatagcgcgc cagcgggttg gtattctcgc cgcagatcag
      181 catgccgatc ttgccgatgt tcgtgttact gacgcgaagg ccttcaccgt cgccgttggc
      241 ccagatcagc ttttcgtaaa atgtcggcac cagcttgcgg tggtgattga tgaggttgcc
      301 gtcggcatcg atcagaacgt tggtgttcca taggcagccc accgagacgg gcgaacgctc
      361 gctgatgccg attgaaacaa cgatggcatt gtcgcgcgcg gcgtcgcaca gcgcggtcac
      421 ctccggccca gggatttcga tcgaattggc cgccatgcgc ttgaagaaat cgtgactttt
      481 gatgggcgcc tgcaccgcag cccacaccgg aaaaccgggc acgaaggatt cgggaaaggc
      541 gatgagccgg gcgcccgcgc ggctcgcttc ggcaatcagc acgcaggcct tgtcgacagt
      601 cgccggcgcg tcgagaaata ccggcgcgac gtgcgcggcg cagaccttga acttcggata
      661 ggcggatgtc gtcatatgtt gtcacagatc aggcggcggc ggtggacacg ccgccgccaa
      721 cgaggatctt tacacgggca tctgcgacag gttgtgccgc agcggcatat tcttcgcgag
      781 ttcttccttc ggaatccgct tcttcacctc gagtttgccg ttttcgacac ggctaatgta
      841 gcagtcgatg atgccgacgt gatcttcttt gcggatgatc ttgtctccct gcggatgtgc
      901 tagcgagttc ttcagctgga tgccttcgag cgcctggatc gcgccctcga catccttccg
      961 ggtcttgtag cccgaagctt cgatcgcctg cttgatcgcg aaaatatcct cccacgactg
     1021 ccagccgtgg ctcttgtcca tcacgcgcgt cccgccgacc tcgcgtgcat gcacctcgtc
     1081 gatgcccatc cgcttgatga attcgcgatg gaattcgtcg tctttggcct tgagcatgcg
     1141 tgggaagttc tcgaagaagt acaccccttc agcggcgccc tgaatgtcgt caggcgcgat
     1201 cgattcaata ctggccgacg aggaatacat cttcattttc ttgtcgaggc ccatgttctt
     1261 ggcctgcgtg tagaaagcga ccgacagcga tccgatgaag gccggcagca gcacctcgga
     1321 ctcacccgga atctgcgaca agtagggcac gaagtccttg gcatcgagcg ggatcgggat
     1381 gctattcagc actttgccgc ccgacttctc gatgtaatgc ttggattcct ggttggtgct
     1441 ctgcccccag gcatagtcgg cataaatgat cgtccatttc ttgccgaagt tttcgacgca
     1501 ccagggcagg ctcgccgccg cgatggaata ggtgtcggtg ccggtgcgga acgaaaagcg
     1561 cgttcccttc gcgccggtcg cctcggtcgc ttcgccggtc gagaagtaga tcgtcttcag
     1621 ctcggaggcg atgggaattg cagcgagcat gaccccggag tgaaccgatc cgacgatgaa
     1681 ctcggcgttg aggcgttgca caagattgcg caacttgcgc gcgccgctgg ccggatttga
     1741 ctccgtatct tcggtcgcga gctcaacctt gcggccggca atgccgccgc cctgattgat
     1801 gatgtcgacg gcagccttgg ccgccttgtc gtgccaaagc ccccatgacg aaataacgcc
     1861 ggtgaggtcg caatgatggc cgatcacgat ggggccggat gcctgcgcgt aaacgtccct
     1921 catcagcgtc aggttagcag tgccgagtgc gccgcccgcc aaggcgactt ctaggaaact
     1981 gcggcgggat aacttccttg ccatgctcgt tctccctctt gtgcgatgtt cagtgagaca
     2041 gcccgagata ccgatgcaca atctcgggct gcgacttgag ttcgtgcgag gtaccggaat
     2101 gaacgatggt gcccttttcc atgagaaaca cgtgtgggca gagcttgagc gcggtctcca
     2161 cgttctgttc gaccagcagg atcgaaaccc cgctctgatt gatccggtga atttcgcggc
     2221 gaatctgaga gaccagccgc ggcatgatgc cttcggtcgg ctcgtccagg atgatcagtt
     2281 tcggccgcat catcaggcag cgggcgatcg cgagcatttg ctgctcgccg ccggaca
//
LOCUS       DASSRL010000185         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1177367, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000185 DASSRL010000000
VERSION     DASSRL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177367"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFL49_03890"
     CDS             complement(<1..339)
                     /locus_tag="VFL49_03890"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7911144.1"
                     /translation="MNREPIFNIPPVVLAVLAVLALVHLARTLLLTEQQDIEFLLTFA
                     FIPARYDNSVVLGGALPGGGADVWTFVTYALIHADWTHFGVNAVWFLPFGSAVARRFG
                     TSRFLVFFAVT"
     gene            742..1347
                     /locus_tag="VFL49_03895"
     CDS             742..1347
                     /locus_tag="VFL49_03895"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7911145.1"
                     /translation="MKHPSTRLIYEYWNRQRGERPAPTRSEIDPAAIRHALGDTFMLA
                     ADFIDGIRFRLAGTRVCALFGREIKGEGFNALWSEASRAQVAGIVDAVVNEAIGAVAG
                     VAGRTEKGTETELELLLLPIGHDERSRVRAIGALVPMAPPYWLGEEAVADLELQTLRH
                     IGAEQSTTRVARFQRGVSQRLRHGFTVYSGGREIGPGNGTN"
     gene            1417..2040
                     /locus_tag="VFL49_03900"
     CDS             1417..2040
                     /locus_tag="VFL49_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7911146.1"
                     /translation="MALPLTKPVILPLAEERRRHQRVKVNLLGRYMLADRREFPCQVV
                     DMSPGGMALHAPMAGQPGERVIAYVDHLGRLEGKIARTFDNGFAMTISATLRKRDKLA
                     AQLTWLANRQILNLPEDRRHGRFAPRNPMARLVLPNGTNVACRVIDLSQSGAAIGIAA
                     DLRPPIGVVVTVGKTQGRVVRHIDDGFAIEFMRLQHPDFVEENVTGE"
     gene            2387..3061
                     /locus_tag="VFL49_03905"
     CDS             2387..3061
                     /locus_tag="VFL49_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HET7911147.1"
                     /translation="MARRLKQALRTGATLAFVAVAMLAASAGATAAKERTASLGSPFP
                     PPEHPLYVAVGEPTRPPIGWVDFCIEYQPECATKASAPRDVVLSPKAWADMVKVNAWA
                     NQAIKPITDMEHWGVIERWNYPDDGYGDCEDYVLLKRRMLMEAGWPREALLITVVRDK
                     KGDGHAVLTVKTDRGEFILDNQEPQILSWERTGYRFVKRQSQSDPNIWVLLGEPPAVP
                     AIVSAR"
     gene            3280..3741
                     /locus_tag="VFL49_03910"
     CDS             3280..3741
                     /locus_tag="VFL49_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7911148.1"
                     /translation="MSQRIDVTKVSPAAFQAVAALQSYVDQSGLDPKLRELIKIRASQ
                     INGCAYCLAMHTRDARKLGETDERMHLLDAWREAPFYTARERAALAWIEAITLVSEGH
                     VPDEVFELVRKQFSEKEIVDLTAAAAAINTWNRLAIAFRVPPQVETRKAAA"
     gene            complement(3742..>3884)
                     /locus_tag="VFL49_03915"
     CDS             complement(3742..>3884)
                     /locus_tag="VFL49_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7911149.1"
                     /translation="DNSLIVGAPARAIRTLDEKAVAMIRAGADIYVQRSKRYAKDLKR
                     IG"
BASE COUNT          773 a         1228 c         1169 g          714 t
ORIGIN      
        1 tgtgacggcg aaaaaaacca gaaagcgcga cgtgccgaat cggcgcgcga ccgcgctgcc
       61 gaacggcaag aaccacaccg cgttgacgcc aaaatgtgtc cagtcggcgt gaatcagcgc
      121 gtaagtgaca aaagtccaga cgtcggcgcc gccgccgggc aacgcaccac cgagcacgac
      181 cgagttgtcg taacgcgccg gaatgaaagc aaatgtcagc agaaattcga tgtcctgctg
      241 ctcggtcaac agcaatgtgc gggcgagatg caccagcgct aacactgcta gcacagcgag
      301 aacgaccggc gggatattga aaatcggttc gcgattcact cacgaggttc cggtgggacc
      361 catgagttac gccgcttggc cggctagccg caagtcggat cagcgacaaa tgaaaaggga
      421 gggctttcgc cctcccttga gccacgttgc ggggttattg ccgcctttgc ggctggtcac
      481 atcccctcgc ctccccgggc tcttgatagt ttttgcgtgc tgccaggtag tgctcggttt
      541 ctcgctgacc aaaggaccac aggcgaccct gcttgccaat ccgactctca ttttaacgct
      601 aaccgcgggc cgccaccgcc aaataccgcg cgcggcacgg tccctgctcc acatccgctg
      661 ccgctgcgtc atcaccagcg cgcctgtgcc gatcggggac agacgctgca attgacgtct
      721 cgcggcggaa caggatgtca cgtgaagcac ccttcgaccc ggcttattta tgagtattgg
      781 aaccggcagc ggggcgagcg acccgcgccg acccgttccg agatcgatcc agcggccatc
      841 cggcatgcac tcggcgatac ttttatgctg gcggccgact tcattgacgg gattcggttc
      901 cggcttgccg gcacccgcgt atgcgccttg tttggccgcg aaattaaagg tgaaggcttc
      961 aatgcgctgt ggagcgaagc gagccgcgcg caggtcgcgg gcattgtcga cgcagtcgtt
     1021 aacgaggcga ttggcgccgt ggccggcgtc gccggacgca cagaaaaagg cacggaaacc
     1081 gaactggaac tgctgctgct gccgatcggc cacgatgaac gctcgcgcgt tcgcgccatt
     1141 ggcgcgctcg tcccgatggc gccaccttac tggcttggcg aagaggcggt ggctgatctc
     1201 gaactgcaga cgctgcgcca tatcggcgcc gagcaatcca ctacccgcgt cgcgcgattt
     1261 caaaggggtg tgagccagcg cctgcggcat ggctttacgg tctacagcgg cgggcgcgaa
     1321 attgggccgg gcaacggcac aaactaacgc aacgttaacg ataggctctt aggctcgtta
     1381 gctgcggaaa ataaacgctt tccgatcccg acagctatgg cactgcccct gaccaaaccg
     1441 gtaattctgc ctctggccga ggaacgccgg cgtcatcagc gcgtgaaggt caacctgctc
     1501 ggccgctaca tgctggccga ccggcgcgaa tttccgtgtc aggttgtcga catgtccccc
     1561 ggcggcatgg cgctgcacgc tcctatggcc ggccagcccg gcgagcgcgt gatcgcctat
     1621 gtcgaccatc tcggccgtct tgagggcaag atcgcacgca ctttcgacaa tgggtttgcg
     1681 atgacgatct cggctacgct gcgcaagcgt gacaagctcg ccgcgcagct tacctggctt
     1741 gccaaccggc agatcctcaa tcttccggaa gatcgccggc acggccgttt tgcgccccgc
     1801 aatccgatgg cccggctggt gctccccaat ggcaccaatg tcgcctgtcg tgtgatcgac
     1861 ttgtcgcaat ccggagccgc gatcggcatt gctgccgact tgcggccgcc gatcggcgtc
     1921 gtcgtcaccg tcggcaagac gcagggccgc gttgtgcgtc acatcgatga cggctttgcc
     1981 atcgagttca tgcggctgca gcaccccgat ttcgttgaag aaaacgtgac cggcgaatag
     2041 cgtcaccggg ccgtaaatgc ggtgcgcaga attgcgcgac cgcggtaaca ggatgctagc
     2101 cgcgcgcgtt cgccggctaa ctgcgacgcg caaatccgtg acatggcaga agtaaacgtc
     2161 agcgcgcggc atgcggcgtc atcgcgacat ttcgcgcgtc atccgttaat gcgaacaatt
     2221 ttacttaaat cgtattggct aagattttag caaaaacaga ctcaatattt atcagtatta
     2281 tctcagagtt ttactatcat tctactcaaa ctgacttgca caacttagcc gcaatacaag
     2341 ccgtttgtgt gaaatgtggc cccaacaaga cgggaagggg ccacgaatgg ctaggcgtct
     2401 caaacaggca ctgcggaccg gcgccacgct ggcgttcgtg gccgtggcca tgctcgcggc
     2461 ctctgccggc gcaaccgccg ccaaagagcg cactgcgtcg cttggtagcc cgttcccgcc
     2521 gccggaacat cccctttatg tcgcagtcgg ggaaccgacg cggccgccga tcggctgggt
     2581 cgatttctgc atcgaatatc agccggaatg cgccaccaag gcatccgcgc cgcgcgacgt
     2641 cgtgctctcc cccaaggcct gggctgacat ggtcaaggtc aacgcctggg cgaaccaggc
     2701 aatcaagccg atcaccgaca tggagcactg gggcgtcatc gagcgctgga actatcccga
     2761 cgacggttac ggcgactgcg aggactacgt cctgctcaag cggcgcatgc tcatggaggc
     2821 cggatggccg cgcgaggcgc tgctcatcac cgtcgttcgc gacaagaaag gcgacgggca
     2881 cgccgtgctc acggtgaaga ccgatcgtgg cgaattcatt ctcgacaatc aggagccgca
     2941 aatcctgtcg tgggagcgca ctggctaccg cttcgtgaaa cgccagtcgc aatccgaccc
     3001 gaacatctgg gtcctcctcg gcgaaccgcc tgccgtgccc gcgatcgtct cggcgcgctg
     3061 acaagaaagg aattcgcgtt cccggtcacg tccccacccc tccccgtccc cagaccgggt
     3121 tcgcgagcgg ccgggcctcc cccaaggccc ggccgcacat tttagaaagc ggatttcgcg
     3181 ttcccttatc cacgtgatgg tcacgaagcc gtcagactgc cggcattttt tgcgcgcaat
     3241 ctttgcaggc ggggcgacgt cagacatcac gggttgctca tgtcacagcg aattgacgtc
     3301 acgaaagtga gcccagccgc gtttcaggct gtggctgcac tgcagtctta tgtcgatcag
     3361 tccgggcttg atccgaaact gcgcgagctc ataaaaatcc gcgcgtcgca aatcaacggc
     3421 tgcgcctact gtctggccat gcacacgcgc gacgcgcgca agcttggcga gaccgacgag
     3481 cgaatgcacc tgctcgatgc gtggcgggaa gcgccgttct atacggccag ggaacgcgcc
     3541 gcgctcgcgt ggatagaggc gatcacgctg gtcagcgaag gccacgttcc ggacgaggtc
     3601 ttcgaactgg tgcgcaagca gttctcggaa aaagagattg tcgatctcac cgccgcagcg
     3661 gccgcgatca atacctggaa ccgattggca atcgcgttcc gtgtcccgcc gcaggtcgag
     3721 accaggaaag cggccgcctg atcagccgat ccgcttgagg tcctttgcgt aacgcttgga
     3781 ccgctggacg tagatatcgg cgccggcgcg gatcattgcg accgcttttt cgtcgagtgt
     3841 gcggatcgca cgcgccggcg cgccgacaat cagcgaattg tccg
//
LOCUS       DASSRL010000186         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_984853, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000186 DASSRL010000000
VERSION     DASSRL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984853"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFL49_03920"
     CDS             <1..357
                     /locus_tag="VFL49_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911150.1"
                     /translation="MNVILIEHNKLWFAAKARNWRLAEYQLGEIKEVMGDVQDLVPTF
                     KNLPLADMLDAVITKEIVALEKAIEAKDYKAFVSGYDKLNQACNACHQGTENDFIVIQ
                     RPTRPAFTNQVYERRR"
     gene            420..1154
                     /locus_tag="VFL49_03925"
     CDS             420..1154
                     /locus_tag="VFL49_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7911151.1"
                     /translation="MTSPDDLKQFAPTGTLRGGVVTAPAASAFFAIRDGEGEVRGVTV
                     DLIRGFAAALKLPLVLQVYDNSGQVTDAVAKGDCDLAFMPQDAERAKRVDFGPAYYFI
                     ESTYLVPAGSKIDSIEEVNRPGHRIVAISNTTTARSARRTAPNASVEEVPSVDKMTEM
                     ARQGQGDAFALSHDSFAGLLPKLPGARVLPGNFQQTGIAVAVPKGRPVALQIASELVE
                     LAKTSGLVRRALDAAGFSDAVVAPRR"
     gene            complement(1171..2145)
                     /locus_tag="VFL49_03930"
     CDS             complement(1171..2145)
                     /locus_tag="VFL49_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HET7911152.1"
                     /translation="MKAAYYERNGKARDVLKVGDVDAPRPGPGEVRVKLAVSGVNPSD
                     VKSRQGSTRKIAYPRVIPHSDGAGVIDEIGEGVSKTRIGERVWVWNGQWKRPFGTAAE
                     FIVLPAMQTVLLPEKVTFEAGACLGIPAMTAAHAVDLAGIGKGTTVLISGGAGSVSQY
                     VLQFAKAKGAVCLATISSADKAKAAREAGADHIIDYKRDNVGDHVVELTDRRGVDVLI
                     EMDLTANVKWIPAVMRPKGSVIVYGTGPEATLPAAFCLVNSIRMQFFLVYEIDATERE
                     RAIATIDNALKQGSLINRLAQPTYKLEDIAAAHEAVERGSLGNVLVTP"
     gene            2362..2544
                     /locus_tag="VFL49_03935"
     CDS             2362..2544
                     /locus_tag="VFL49_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911153.1"
                     /translation="MLYKELHTLDEALSWARHVEQTGRSPLLIEGDDGTRMNRRAIVD
                     ALRVGMREDVDGQADA"
BASE COUNT          656 a          869 c          898 g          630 t
ORIGIN      
        1 atgaacgtca ttctgataga gcacaataaa ctctggttcg ccgccaaagc gcgcaattgg
       61 cggctcgccg aataccaact cggcgaaatc aaggaggtca tgggcgacgt gcaggacctc
      121 gtgccaacat tcaagaacct gccgttggcc gacatgctcg acgccgtcat caccaaggaa
      181 atcgtcgcgc tggaaaaagc aattgaagcc aaggattaca aagctttcgt cagcgggtac
      241 gacaagctga accaagcctg caatgcctgc caccagggca cggaaaatga ctttattgtc
      301 attcagcgcc cgacgcggcc cgccttcacg aatcaagtct atgagcgccg tcgctgattt
      361 ggctcgattg aatgaggcgg tatagccgtg tacaggcggt cgtgttagac gcaccaagta
      421 tgaccagtcc cgacgatctc aaacaattcg cccctacggg gacgctgcgt ggcggcgtcg
      481 tgactgcacc tgcggcatcc gcattttttg cgatcaggga cggcgaagga gaggtcaggg
      541 gtgtcaccgt cgacttgatc cgcgggtttg ccgcggcact gaaactgccg ctggttcttc
      601 aggtctacga caattccggt caggtgactg acgccgtcgc caagggggat tgcgaccttg
      661 ccttcatgcc gcaggatgcc gagcgcgcga aacgcgtgga ttttggccct gcatattatt
      721 ttatcgagag cacatatctt gtgccggcag gctcgaagat agacagcatc gaagaggtca
      781 accggccggg ccacaggatc gtggcaattt cgaacacgac gacggcccgc agtgcgcgcc
      841 gcacagcgcc gaatgccagc gtcgaggaag tgccgagtgt cgacaagatg acggagatgg
      901 cgcggcaagg acagggggac gcgttcgcgc tgtcgcatga ctcatttgcc ggtctgctgc
      961 cgaagcttcc aggtgcacgc gtccttccgg gcaatttcca gcagaccggg atcgcggttg
     1021 ctgtgccgaa ggggaggccg gtcgcgctgc agatcgcgag cgaattggtg gagttggcca
     1081 agacctcagg tctggtgcgc cgcgcgctcg atgcagccgg attttccgat gccgtggtag
     1141 cgccgcgccg ctgacgccga gactgcagtt tcagggtgtc acaagcacgt tgccgagact
     1201 tccgcgttca acggcttcgt gagcagcggc gatgtcttca agtttgtatg ttggctgcgc
     1261 cagccgattg attaatgagc cttgcttcaa ggcgttgtcg atggtcgcga ttgcccgttc
     1321 acgctcagtt gcgtcaatct cgtaaaccag aaaaaactgc attcggatcg agttgaccag
     1381 gcagaatgct gccgggagtg tggcctcggg gcccgtgccg tagacgataa cgctgccttt
     1441 cggccgcata accgccggga tccattttac gtttgcggtt agatccattt cgatcaagac
     1501 gtcgacgccg cgcctatcgg tcagttccac gacatgatcg ccgacgttgt cgcgcttgta
     1561 atcgataatg tggtccgcac cggcctctcg tgcagctttg gctttgtcag cagaagatat
     1621 cgttgcgagg cagaccgcgc ctttagcctt cgcaaactga aggacatact gactgaccga
     1681 gccagccccg ccagagatca gtaccgtcgt gcctttgccg attcctgcga ggtcaaccgc
     1741 atgcgcagcg gtcatcgccg ggataccaag gcaggcgcct gcttcgaagg tgaccttttc
     1801 cggcagtaaa acggtttgca ttgcgggaag cacgatgaat tcggcagctg tgccgaaagg
     1861 gcgtttccat tggccattcc agacccagac ccgctccccg atccgggttt tcgacacccc
     1921 ttcaccgatt tcgtcaatta ctccggcgcc gtcgctatgg ggtatcacgc gcggataggc
     1981 gatttttcgt gtggaaccct gtcgcgactt gacgtcagaa ggattgaccc ccgacacggc
     2041 gagtttgacg cgaacttcgc ctgggcccgg gcgcggcgcg tcgacatcgc cgaccttgag
     2101 gacgtcgcga gccttgccgt tgcgttcgta ataagccgct ttcatgagct gcccctctgc
     2161 cgtcacgata gcatgccatt cccgccaaga aatcgtcggg agcgaaaccc cgccgcggct
     2221 cgcacgttac cttttttggg caaattcgag gggtaggccg cccggaaagg gcggcgacag
     2281 gccacgaggt tgatgatgac ttatcgcgtg tatagcggac caaaggggtc tgcggatgta
     2341 tcaccgttcg aaaaatcgca aatgctgtac aaggagttgc acacgctcga cgaagccctt
     2401 tcatgggcgc ggcatgtgga gcagaccggc aggtcccccc ttttgattga aggcgacgat
     2461 ggtacacgaa tgaaccgtcg cgcaattgtg gacgcgctac gggtgggtat gcgtgaggac
     2521 gttgatggac aggcggacgc ctaaattttg ttgcggatta gcttgcaatc cgattccact
     2581 ctgcccgtgc cctctccgtg aggtggttcc atttcgtact gaccacatcc cagttcacca
     2641 taggccgctg tgcgctggct gaggaattgg ccgcaccaag gggcgtacgt gaatcgtaca
     2701 agtaggcacc gcctacggtc agcacagcgc caagaatgat cccgatgagg acgcgcatga
     2761 aatgatctcc ttgtccccca caaaaacgcg gccgcaaacc agcggttcca tggctcggtg
     2821 gaccgatttt ggcagaaaac cggactattt taggtcagac gtcattaacc ttggatactc
     2881 accaaccgtg ttctaactta ttgaaaatgc taacgttatc gcggcaaaaa ctttgcccgc
     2941 cagcaaaatt tgctgctata ttgaaattat gagcagacat tcagcaaaag ccgccggacg
     3001 gtccgcaaaa gccgcccgag gcaccgccaa gaagagtacg aaaagcgtac gtt
//
LOCUS       DASSRL010000187         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_810048, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000187 DASSRL010000000
VERSION     DASSRL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810048"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFL49_03940"
     CDS             complement(<1..606)
                     /locus_tag="VFL49_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7911154.1"
                     /translation="MSVPGVAVSNENVAPEIRSITILGATGSIGASTIDLIKREPGRY
                     RVESISARRNASALGKIAREVGARHAVVADPAAYGDLKSALSGSRIEAAAGEEALIEA
                     AQRPADWVMAAISGAVGLKPTMAAIERGATVALANKECLVCAGSLFMRRAASRGATVL
                     PVDSEHNAVFQALGSGNRADVRRAILTASGGPFRPWSKEAIK"
     gene            complement(612..1475)
                     /locus_tag="VFL49_03945"
     CDS             complement(612..1475)
                     /locus_tag="VFL49_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7911155.1"
                     /translation="MVGIDPVSNAEKPGARLSANNLMLRVVSAAILAPLALLAAYAGG
                     WPFALFWGIAALVVLWEWMTLVAGPGYRLMVSSCAAAIAVADFLAWLGRPVTALCVVG
                     LGALAGAIFAPGERRLWVIAGTGYAGGLALAPIFVRADNGFGFVAIVLLFAIVWTTDV
                     IGYFAGRAFGGPKLWPAVSPKKTWSGAIAGTIAAVAVALLVAGQFGSFGRIAIVAVAL
                     LLSIVAQGGDLLESWVKRRFDAKDSSQLIPGHGGAMDRLDGFWAAAVAGCVVGLFRGG
                     FDEPARGLLIW"
     gene            complement(1479..2231)
                     /locus_tag="VFL49_03950"
     CDS             complement(1479..2231)
                     /locus_tag="VFL49_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7911156.1"
                     /translation="MPETEIHPDATAFDIPRHVAIIMDGNGRWAATRGLPRAEGHRRG
                     VEALRRTVRAAGEIGIKILTIFSFSAENWSRPASEIGELMGLLRRFVRNDLAELHQNN
                     IRVRVIGERADLDPDIGRLLVEAEELTKNNDGLTLVVAFNYGARQEIVRAARRIVQAA
                     DRGETKAGDIDMDTFARFLDAPDIPDPDLIIRTSGEQRLSNFLLWQAAYSELVFVPTF
                     WPDFDRGALESAIREYRQRERRFGGLVAQTGS"
     gene            complement(2243..>2481)
                     /locus_tag="VFL49_03955"
     CDS             complement(2243..>2481)
                     /locus_tag="VFL49_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HET7911157.1"
                     /translation="ERRRELVKVAHKYAETARVAVRHVRRDAMDVIKKLEKDHKISKD
                     DHDRFSTDIQKATDAGIAEIDHMLAAKEKEILTV"
BASE COUNT          493 a          844 c          716 g          428 t
ORIGIN      
        1 cttgatggcc tctttcgacc acggccggaa tggcccgccc gatgccgtga gaattgcgcg
       61 cctgacgtcg gcacgatttc ccgaaccaag cgcctgaaaa accgcgttgt gctcggaatc
      121 gaccggcaga acggttgccc ctctgctcgc ggcccggcgc atgaacaagc tgcccgcaca
      181 gacgaggcat tccttgtttg ccagtgccac cgtcgcgccg cgctcgattg cggccatcgt
      241 cggcttgagc ccgaccgccc cgctgatcgc ggccatcacc cagtcggccg ggcgctgcgc
      301 agcctcgatc agagcctcct cgccggccgc cgcctcgatg cgcgagccgg aaagcgcgct
      361 cttgagatcg ccataggcgg ccgggtcggc gacgacagcg tggcgcgcgc ccacttcacg
      421 ggcaattttt cccagcgcac tcgcattgcg gcgcgcgctg atcgactcga cgcgatatcg
      481 cccgggctcc cgcttgatca gatcgatggt gctggcgccg atcgaaccgg tcgcaccgag
      541 aatggtaatg ctccggatct ccggcgcgac attttcgttt gaaacagcga cgcccggtac
      601 gctcatgtgc ctcaccagat caacaaaccg cgcgccggct catcaaagcc gccgcgaaat
      661 aacccaacca cgcaacctgc aactgccgcc gcccagaacc cgtcgagccg atccatcgcg
      721 ccgccgtgtc ccggaatgag ctggcttgag tccttggcgt cgaaccggcg cttcacccag
      781 gattcaagca agtcgccccc ttgcgccaca atggacagta acaatgccac agcgacgatc
      841 gcaattctgc caaacgagcc gaattggccg gcgacgagca atgcgacagc gacagcggcg
      901 atcgtgcccg cgatcgcccc tgaccaggtc ttcttcggac tcacggccgg ccatagcttg
      961 ggaccgccga atgcacgtcc ggcaaaatag ccaatgacgt cggttgtcca gacaatcgca
     1021 aaaagcagca cgatcgcgac aaatccgaag ccgttgtcgg cccttacgaa tatcggcgcc
     1081 agcgccagac cgcccgcata gccggtaccc gcgatgaccc aaagccgccg ttcgcccggc
     1141 gcaaaaattg cccccgccag ggcgcccagg ccgaccacgc acagcgccgt aacgggcctg
     1201 cccagccagg cgagaaaatc tgctacggca atagccgccg cgcaggacga caccataagg
     1261 cggtagccgg gtcccgcgac cagcgtcatc cactcccaaa gcaccacaag tgccgcaatt
     1321 ccccaaaaca gcgcgaatgg ccagccgccg gcataagccg caagcagcgc cagcggggca
     1381 aggattgcgg ccgacacaac gcgcaacatg agattgttcg cggatagtcg cgcgccgggc
     1441 ttctccgcgt tcgacacggg gtcaatgccg accacggatc acgatccggt ctgggcgacg
     1501 aggccgccga accgccgctc gcgttgccga tactcgcgga tcgccgattc aagcgcgcca
     1561 cgatcgaaat caggccaaaa agtcggaaca aaaaccagtt cgctgtaggc cgcctgccag
     1621 agaagaaaat tcgacaagcg ctgctcgccg cttgtgcgga tgatcaggtc cggatcgggg
     1681 atgtcgggcg catcgagaaa ccgcgcgaac gtgtccatgt cgatgtcacc ggccttcgtc
     1741 tcgccacgat ccgccgcctg gacaatccgg cgcgctgcgc ggacaatttc ctgccgggcg
     1801 ccgtagttga acgccacaac caatgtcagc ccgtcgttgt tcttggtgag ctcttcggct
     1861 tcgacgagca accgcccgat atcgggatcg agatccgcgc gctcgccgat cacccgaacg
     1921 cgaatgttgt tctggtgaag ttcggcaaga tcgttgcgca cgaagcggcg caacagtccc
     1981 atcagctctc cgatttcact tgcaggacgc gaccagtttt ccgcgctgaa agagaaaatg
     2041 gtgagaatct tgattccgat ctcgccggcg gcacgtacgg tgcgacgaag cgcttccact
     2101 ccgcgacggt gaccttcggc gcgcggcaga ccgcgcgttg ccgcccagcg gccattgcca
     2161 tccatgataa tggctacatg gcgcggaata tcgaatgccg ttgcatccgg atgaatttcg
     2221 gtctcgggca tgcaacgcga cctcagaccg tgaggatctc cttttccttt gcggccagca
     2281 tatgatcgat ctcggcgatg ccggcgtcgg tcgctttctg aatgtcggtc gaaaagcggt
     2341 cgtgatcgtc tttgctgatc ttgtggtcct tctccaattt tttgatgacg tccatggcgt
     2401 cgcgcctgac atggcgcacg gctacacggg cggtttcggc atatttgtgc gccactttca
     2461 ccaattccct gcggcgttcc t
//
LOCUS       DASSRL010000188         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_984896, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000188 DASSRL010000000
VERSION     DASSRL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984896"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFL49_03960"
     CDS             <1..668
                     /locus_tag="VFL49_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911158.1"
                     /translation="AKVIATDITLEKLDGLKAAKTAKLDVLSTDAVNTLAASIDKEFG
                     ALSILVNVAGYVHQGTVLECSEKDWDLSFDLNVKSMHRTISAFLPAMLKNGGGSIINM
                     SSGVSSIKGLPNRYAYGATKAAVIGLTKAVAADFITKGIRCNAICPGTIVSPSFEERV
                     QAVAKQTGTPLDQVRKNFIARQPMARLGTAEEVAALAVFLASDESSYITGQPHLVDGG
                     MAL"
     gene            830..1897
                     /gene="ugpC"
                     /locus_tag="VFL49_03965"
     CDS             830..1897
                     /gene="ugpC"
                     /locus_tag="VFL49_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7911159.1"
                     /translation="MASVAIRDVRKAFGATSVIHGVDISIGDGEFVVLVGPSGCGKST
                     LLRMIAGLENITAGEIRIGERVVNQVPPKERDVAMVFQNYALYPHMTVADNMAFSMKL
                     RGAPKSEIDERVNRAAVILGLSQLLHRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTTIYVTHDQIEAMTMADKIVVMHDGLVEQMGAP
                     LDLYDRPDNLFVAGFIGSPAMNMLNGRIRANGSAVFEGPEGVKFMLGAAPPGSDGRPA
                     VFGVRPEHFALADDGAEAEVQVVEPTGSELQVVARLGGNDIIAVFRERHQFKPGDKIR
                     LKPDPRLVHLFDEQSGKRMTS"
     gene            1918..>3159
                     /locus_tag="VFL49_03970"
     CDS             1918..>3159
                     /locus_tag="VFL49_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7911160.1"
                     /translation="MDILTRRALVKGGTALAAAGTLTGPALLDWAKAWAQSAPWKPEK
                     GAKLSMLRWKYFVQAEDDAFVKLIQAFTQATGVPVEISRESYEDVQPKASVAANTGTG
                     PDLFWGLYSLPHLFPQKCLDVTDVADYLGKAHGGWVDSAIKYGKGTGSKWIDIPICYS
                     GALINYRLEASKKAGFPKFPTTTDAFLEYAKAMKAQNTPGGFALGHASGDGNTWVHWC
                     LWTHNGQVVDKNDKVIINSLETVKALEFAKNLYGNMIPGTASWNDASNNKAFLAKEIH
                     WTNNGISIYVAAQNNAKDVAADMDHAYFPIGPAGKPTELHLMYPILAMTYTKYPQACK
                     ALMAFMLDANNFNPWIQSAKGYLTHCLNEYDKNPIWTADPKNTPYRDVAKRSLTAGGL
                     GSVGEKAATAIADFVVLDMFAN"
BASE COUNT          669 a          980 c          945 g          565 t
ORIGIN      
        1 gcgcgaaggt cattgcaacc gacatcacgc tcgaaaagct tgacggttta aaggctgcga
       61 aaacggccaa actcgacgtg ctgtcgaccg acgcggtgaa taccctggcc gcatcaatcg
      121 acaaagaatt cggcgcgctc agtatacttg tcaacgttgc cggctatgtg catcagggaa
      181 cggtgctgga gtgctcggag aaggactggg acctctcctt cgacctcaac gtcaaatcca
      241 tgcaccgcac gatcagcgcc ttcctgcctg ccatgctgaa gaacggcggc gggtcgatca
      301 tcaacatgtc gtcgggcgtg tcgtccatca aaggcctgcc caaccgttac gcctatggcg
      361 cgaccaaagc ggccgtgatc gggctgacca aagccgtcgc cgcagacttc ataacgaagg
      421 gcatccgctg caacgccatc tgccccggca cgatcgtgtc gccctcgttc gaggagcgcg
      481 tgcaggccgt cgccaagcag acaggaacgc cgctcgatca ggtgcgcaag aatttcatcg
      541 cacgccagcc gatggcccgc cttggcaccg ccgaggaagt cgccgccctt gccgttttcc
      601 tggcttcgga tgaatcgagc tacattaccg gtcagcccca tctcgttgac ggcggcatgg
      661 ccctgtaaag gtgcccaccg ttattgccca ccggaccata agtcgggctt gcatttcgaa
      721 gggcgaaaga aggatgattt gccctgacgg atgttccagc ggccgcctga ttgatggccc
      781 tgaaaacaaa aaagaatatg tgtaaggctg cccggcctgg gaggggtgaa tggcatcggt
      841 cgcgatccgc gacgtccgca aggccttcgg tgcgacaagc gtcatccatg gcgtcgatat
      901 ctcgataggc gacggcgaat tcgtcgtgct ggtcgggccg tcgggctgcg gcaagtcgac
      961 cttgctgcgc atgatcgcgg ggcttgaaaa catcactgcc ggcgaaatcc gcatcggcga
     1021 gcgcgtagtc aaccaggtcc cgccgaagga acgcgacgtc gccatggtct tccagaacta
     1081 cgcgctctat ccgcacatga cggtcgctga caacatggca ttctccatga aattgcgcgg
     1141 cgcgccgaaa agcgagatcg atgaacgggt caatcgagcc gccgtgatcc tcggtctctc
     1201 gcaattgctg caccgctatc cgcgtcagct gtcgggcgga cagcggcagc gcgttgcgat
     1261 gggtcgtgcc atcgttcgcg atccgcaggt ttttcttttc gacgagccac tgtccaacct
     1321 cgatgccaaa ttgcgcgtgc agatgcgcac ggagatcaag gaactgcacc agcggctgaa
     1381 gaccaccact atctacgtca cgcacgacca gatcgaggcg atgaccatgg ctgacaagat
     1441 cgtcgtcatg cacgacggcc tggtggagca gatgggcgca ccgctcgatc tttatgaccg
     1501 cccggacaat ctgtttgttg cgggctttat cggctcgccg gccatgaaca tgctcaacgg
     1561 ccgcattcgt gccaatggca gtgccgtctt cgaagggccc gagggcgtga aatttatgct
     1621 cggcgcggcg ccccccggca gcgacgggcg gccggcagtg ttcggcgtgc ggcccgagca
     1681 tttcgctctc gccgacgatg gtgccgaggc ggaagtgcag gtggtggaac caaccggctc
     1741 ggagctccag gttgttgcca ggcttggcgg caacgacatc atcgcggtgt tccgcgaacg
     1801 tcaccaattc aagccgggcg acaagatccg gctcaagcca gacccgcgcc tcgtccatct
     1861 cttcgacgaa caatccggga aacgaatgac cagctaggaa ccaacggagg aacgaccatg
     1921 gacattttga ctcgccgcgc tttggtaaag ggcggcacgg cgctggcggc agccggcacc
     1981 ttgaccggac ccgcactact cgactgggcg aaagcctggg cgcagagcgc gccctggaag
     2041 ccggagaaag gcgccaagct ctcgatgctg cgctggaaat acttcgttca ggcggaagac
     2101 gacgctttcg tgaagctcat ccaggccttc acccaggcca ccggcgttcc ggtcgagata
     2161 tcgcgcgagt cctatgagga cgtgcagccg aaggcgtcgg tcgccgccaa caccggcacg
     2221 ggcccggacc tgttctgggg tctttattcg ctgccacatc tgttcccgca gaagtgcctc
     2281 gatgtcaccg acgttgccga ctatctcggc aaggcacatg gcggctgggt cgacagcgcg
     2341 atcaaatacg gcaagggcac cggttcgaaa tggatcgaca ttccgatctg ctattcgggc
     2401 gcgctgatca attaccggct tgaagcgtcg aagaaggccg gcttcccgaa attcccgacc
     2461 acgaccgacg cgtttctcga atatgccaag gcgatgaagg cacagaacac acccggcggc
     2521 ttcgcgcttg gccatgcgtc gggtgacggc aacacctggg tgcactggtg cctgtggacc
     2581 cataacggcc aggtcgtcga caagaacgac aaggtgatca tcaactcgct ggagacggtc
     2641 aaggcgctcg aattcgccaa gaacctctac ggcaacatga tcccgggcac ggcctcgtgg
     2701 aacgacgcct ccaacaacaa ggcgtttctt gcgaaggaaa tccactggac caacaacggc
     2761 atctcgatct atgtcgcggc gcaaaacaac gcgaaggacg tggcagcgga catggatcac
     2821 gcctacttcc cgatcggtcc ggccggcaag ccgaccgagc tgcacctgat gtatccgatc
     2881 ctggcgatga cctacacgaa gtatccgcag gcctgtaagg cgctgatggc tttcatgctc
     2941 gacgccaaca acttcaatcc ctggatccag tcggccaagg gctatctcac gcactgcctc
     3001 aatgagtacg acaagaaccc gatctggacg gccgatccga agaacacgcc atatcgggac
     3061 gttgccaagc gctcgctcac cgcgggtggc cttggctcgg tcggagagaa ggcggccact
     3121 gcgatcgccg atttcgtcgt gctcgacatg ttcgccaac
//
LOCUS       DASSRL010000189         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_57751, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000189 DASSRL010000000
VERSION     DASSRL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57751"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFL49_03975"
     CDS             complement(<1..660)
                     /locus_tag="VFL49_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET7911161.1"
                     /translation="MNIHEYQAKAVLREFGVPVPRGVPAFTVDEAVNGAKELGGPVWV
                     VKAQIHAGGRGKAGGVKVVKSIDDVKKESTRILGSTLITHQTGPQGKKVNRLYIEEGS
                     AIDREFYLSALIDRETSRVAFVVSTEGGMDIEEVAHKTPEKILTFSVDPATGIMPHHG
                     RRVAQALKLSGDLAKQIEAILPKLYAAFTSKDMSLLEINPLVATKDNKIVCLDAKVGF
                     DG"
     gene            complement(726..1691)
                     /gene="mdh"
                     /locus_tag="VFL49_03980"
     CDS             complement(726..1691)
                     /gene="mdh"
                     /locus_tag="VFL49_03980"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7911162.1"
                     /translation="MARNKIALIGAGQIGGTLAHLIGLKQLGDVVMFDVMEGIPQGKA
                     LDIAESSPVNDFDAKFVGTNSYDAIEGANVCIVTAGVPRKPGMSRDDLLDINLKVMGQ
                     VGSGIKKYAPDAFVICITNPLDAMVWALQKSCGLPQQKVVGMAGVLDSARFRYFLADE
                     FHVSVEDVTAFVLGGHGDTMVPLIRYSAVAGIPVPDLVKMGWTTQARIDEIVKRTANG
                     GAEIVNLLKTGSAFYAPAASAIAMAESYLLDKKRVLPCAAWLNGEYGIKDLYVGVPVV
                     IGAKGVERIVEIELNGSERTMFEKSAAAVQGLVEACKKIAPNLGN"
     gene            complement(1851..>2053)
                     /gene="zapE"
                     /locus_tag="VFL49_03985"
     CDS             complement(1851..>2053)
                     /gene="zapE"
                     /locus_tag="VFL49_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HET7911163.1"
                     /translation="YDHAVKLLASAQAQPDELYIATEGYEVNEFKRTASRLIEMRSQA
                     YLGLPHGPRQGPAFDAREIVET"
BASE COUNT          383 a          638 c          599 g          433 t
ORIGIN      
        1 accgtcgaag ccaaccttgg catcaagaca gacgatcttg ttgtccttgg tcgccaccag
       61 cggattgatc tcgagcaggc tcatgtcctt gctggtgaag gcggcataca gcttcggcaa
      121 gattgcctcg atctgcttgg cgaggtcgcc gctgagcttg agcgcttgtg caacccgacg
      181 gccatgatgc ggcatgatac cggttgccgg atcgacggag aacgtgagga tcttttcggg
      241 cgtcttgtgc gcgacttctt cgatgtccat tccgccttca gtcgagacca cgaaggcgac
      301 gcgcgacgtc tcgcgatcga tgagtgccga gagatagaac tcgcggtcga tggctgaccc
      361 ttcctcaatg tagagccggt tgactttctt gccttgcggg ccggtctgat gcgtgatcag
      421 cgtcgagcca aggatgcgcg tcgattcctt cttcacgtcg tcgatcgatt tcacgacttt
      481 gacgccgccc gccttgccgc gaccgccggc gtgaatctgc gctttcacga cccacacagg
      541 cccgcccagt tccttggcac cgttgacggc ctcatccaca gtgaatgccg ggacgccgcg
      601 cggcacggga acgccgaatt cgcgcaatac cgcctttgcc tgatattcgt ggatattcat
      661 gaggctcccc aatgtgaaca gtgaattgcg cgcagtcgcg cctctattca ctgtttcgct
      721 gttcgctaat tacccaagtt aggcgcgatc tttttgcagg cctcgaccag gccctgcaca
      781 gccgccgccg atttttcgaa catcgtacgc tcgctgccat tcagttcgat ttcgacgata
      841 cgctcgacac ccttggcgcc gatcacgacc ggtactccaa catagaggtc cttaattcca
      901 tattcgccgt tgagccaggc ggcacaaggc agtacgcgct tcttgtcgag aagatagctc
      961 tccgccatcg cgatcgcaga cgccgccggc gcataaaatg ccgaaccggt cttgagcagg
     1021 ttgacgatct cggcgccgcc gttggctgtc cgtttcacga tctcgtcgat ccgcgcctgc
     1081 gttgtccagc ccatcttcac cagatcgggc accgggattc cggcgaccgc cgaataccgt
     1141 atgagcggga ccatggtatc gccgtgtcca ccgaggacga aggcggtcac gtcttcgacc
     1201 gagacgtgga attcgtcggc gaggaagtag cggaagcgcg ccgagtccag cacgcccgcc
     1261 atgcccacca ctttctgctg cggcaggccg caactttttt gcagggccca gaccatcgca
     1321 tcgagcgggt tggtgatgca gatgacgaac gcgtcggggg cgtatttctt gatcccggaa
     1381 ccgacctgcc ccatgacctt gaggttgatg tcgagaagat cgtcgcggct catgcccggc
     1441 ttgcgcggga cgcccgcggt gacgatgcac acattggcgc cctcgatggc gtcgtagctg
     1501 ttcgtcccga cgaacttggc gtcgaaatcg ttgaccggtg aggactcggc aatatcgagc
     1561 gccttgccct gcggaattcc ttccatgacg tcgaacatca cgacgtcgcc gagttgcttg
     1621 aggccaataa gatgcgccag cgtgccgccg atctggccgg caccgatcaa cgcgattttg
     1681 ttacgcgcca tggtgggaaa ctctcgactt gctgctgccg cggagcgcgg ccgcgaggtg
     1741 gttaaccggt tagatttggc ccgacaaggc accaaaaccg cggccctgca cccttgtata
     1801 cattatacca gcaacgggag gctccgagtc cagagccaaa attcaggaaa tcaggtctcg
     1861 acgatttccc tcgcgtcgaa ggcggggccc tgtctcggcc catgcgggag gccgaggtaa
     1921 gcctgcgacc gcatctcgat caggcgcgaa gcggtgcgct tgaattcgtt gacctcatac
     1981 ccttcggtcg caatgtagag ctcatcgggt tgtgcctgcg ccgaagccag cagcttcacc
     2041 gcatggtcgt aga
//
LOCUS       DASSRL010000190         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_249969, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000190 DASSRL010000000
VERSION     DASSRL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249969"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..158)
                     /locus_tag="VFL49_03990"
     CDS             complement(3..158)
                     /locus_tag="VFL49_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911164.1"
                     /translation="MFSLIPWDVRLAGALMVITGLAAIFDLGSVFLVMLYTMLCAYIL
                     VNTFTKQ"
     gene            complement(240..416)
                     /locus_tag="VFL49_03995"
     CDS             complement(240..416)
                     /locus_tag="VFL49_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911165.1"
                     /translation="MRAFIIGSVIIILLAIGGAFGLHFVQETSSIAYTSPTGAKLDHA
                     ESALNNYGRQVVPD"
     gene            complement(430..1437)
                     /locus_tag="VFL49_04000"
     CDS             complement(430..1437)
                     /locus_tag="VFL49_04000"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HET7911166.1"
                     /translation="MTRLVARLGTFCGAAIFLFVLMAASPVRAQQPASVNPTAAAVQE
                     QQLLKELKRVQGRITIPDAQESVLIQPAGRNWRHFHQVTLHWIGGIAMIGMLVLLVVF
                     YLVRGSIKIASGRSGRTILRFDGLERTVHWMTAVSFIILGISGLNITFGRSLLLPLMD
                     PQTFTNFSEALKYAHNYLSFPFTIGILFMLFMWIKDNIPDRTDIEWLKEGGGMVGDKH
                     PPARRFNAGQKGIFWIVVLGGAAMAISGYLLMFPFYGGTGIADMQWAQVVHGVVGVLF
                     IAAIFAHIYIGTLGMEGAFEAMGEGEVDVNWAKEHHSLWVKEEMGKSPSSTHARMTPA
                     E"
     gene            complement(1463..2059)
                     /gene="fdh3B"
                     /locus_tag="VFL49_04005"
     CDS             complement(1463..2059)
                     /gene="fdh3B"
                     /locus_tag="VFL49_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HET7911167.1"
                     /translation="MARMKFLCDADRCIECNACVTACKNENEVPWGINRRRVVTINDG
                     KPGERSVSMACMHCTDAPCQAVCPVNAFFTTADGVVLHSKDICIGCGYCFYACPFGAP
                     QYPRVGNFGSRGKMDKCTYCAGGPETDNTAAEYAKYGANRLAEGKLPLCAEMCSTKSL
                     LAGDGEIIAQIYKERVMKRGYGSGAWGWQTAYHETIKV"
     gene            complement(2071..>2929)
                     /locus_tag="VFL49_04010"
     CDS             complement(2071..>2929)
                     /locus_tag="VFL49_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET7911168.1"
                     /translation="DGTTVKVSMLADGSFSKDSEIKDGYPQFTVGVLKKLGWDKDLTA
                     AELAVINKVNPANPDAVSWSTDLSGGVQRVALKHGCIPYGNGKARMNAWNLPDPIPVH
                     REPIYSPRVELIQKYPTLPDAKQFRLPNIGFTVQKAAVDKGIAKQFPLILSTGRLVEY
                     EGGGEETRSNRWLAELQQDMFIEINVADASARGIKDGGWVWVTGAENNAKAKVKALIT
                     ERVGKGVAWMPFHFGGWFGGEDLRKNYPAGTDPIVLGESANTITTYGYDPATGMQEPK
                     VTLCQIKAA"
BASE COUNT          551 a          904 c          879 g          595 t
ORIGIN      
        1 gactattgct tcgtgaacgt attcacgagg atgtaggcgc aaagcatcgt gtacagcatg
       61 acgaggaaca ccgacccgag atcgaagatc gccgcgagcc cggtaatcac catgagcgct
      121 ccggcgagcc taacgtccca ggggatcagc gaaaacatca cgcctccttg cggctggcgc
      181 tttgttgttc aggccttcag ccggggcgat cggtcccggc gggaattgcg gctcgtcgat
      241 cagtccggaa cgacctgcct gccgtaattg ttcaaagccg attccgcgtg atccagcttc
      301 gcgccggtcg ggctcgtata ggcgatcgac gaagtctcct gcacgaaatg caggccgaag
      361 gcgcctccaa tcgcgagcag gatgatgatg acggatccaa taataaatgc gcgcatggtc
      421 acccccgaat tattcagccg gcgtcattct ggcgtgcgtg gaggacgggc tcttgcccat
      481 ttcctccttc acccaaaggc tgtgatgctc tttcgcccag ttgacgtcga cttcgccctc
      541 gcccatcgcc tcgaatgcac cttccatgcc gagcgtgccg atatagatgt gcgcgaagat
      601 cgccgcgata aagagaacgc cgaccacacc gtgaacaacc tgcgcccatt gcatgtcggc
      661 aattccggtg ccgccataga acgggaacat caacaggtag cctgagatcg ccatcgcggc
      721 gccgcccagc accacgatcc agaagatgcc tttctgcccc gcgttaaagc ggcgcgccgg
      781 cggatgcttg tcgcccacca tgccgccgcc ctccttgagc cattcaatgt cagtgcgatc
      841 cgggatgttg tctttgatcc acatgaacag catgaacaga atgccgatcg tgaacgggaa
      901 gctcagatag ttgtgcgcgt atttcagcgc ctcggagaaa ttcgtaaagg tctgcggatc
      961 catcagcggg agtagcaagg acctgccgaa cgttatgttg agccctgaaa tgccgaggat
     1021 gatgaagctc accgccgtca tccagtgcac cgtgcgttcg agcccatcga agcgcaggat
     1081 ggtgcggccg gaacggccgc tcgcgatctt gatcgagccg cggacgaggt agaacaccac
     1141 gagcagaacc agcattccga tcatcgcgat cccgccgatc cagtgcagcg tcacctgatg
     1201 gaagtgccgc cagttgcggc ctgcgggctg aatcagcacg ctttcctgcg cgtcggggat
     1261 cgtgatgcgg ccctgtaccc gcttcaactc cttcaggagc tgctgctcct gcacggcggc
     1321 ggcggtcggg ttgacgctcg cgggctgttg tgcgcggacg ggcgatgccg ccatcaacac
     1381 gaagagaaag atcgcggcac cgcaaaaggt gcccagccgg gccacaagcc ttgtcatttt
     1441 tcgttcctcc ctcttcgctc gatcaaacct tgatcgtttc gtgataggcg gtctgccagc
     1501 cccaggcgcc ggagccgtag ccgcgtttca tcacgcgttc cttgtaaatc tgcgcgatga
     1561 tctcgccgtc acccgcgagc aaggacttcg tcgagcacat ctcggcgcag agcggcagct
     1621 ttccttccgc caagcggttg gcgccgtatt tggcgtattc cgcggcggtg ttgtcggttt
     1681 ccggcccgcc cgcgcagtag gtgcatttgt ccatcttgcc gcgtgagccg aaattgccga
     1741 cgcgcggata ttgcggcgcg ccgaacggac aagcgtagaa gcagtaaccg cagccgatgc
     1801 agatatcctt ggaatgcagg accacgccgt cggccgtggt gaagaaggcg ttcaccggac
     1861 acaccgcctg acacggcgcg tcggtgcaat gcatgcaggc catggagacc gagcgttcgc
     1921 ccggcttgcc gtcgttgatt gtcacgacac ggcggcggtt gatgccccaa ggcacttcgt
     1981 tttcgttctt gcaggcggtg acgcaagcgt tgcattcgat gcagcgatcg gcgtcgcaga
     2041 gaaatttcat tcgagccatg gttccgcctc ctatgccgct ttgatctgac aaagggtgac
     2101 tttgggctct tgcatgcccg tcgccggatc gtagccatac gttgtgatgg tgttggcgct
     2161 ttcaccgagc acgatcggat cggtgccggc gggatagttc ttgcggagat cttcgccgcc
     2221 gaaccagccg ccgaagtgga agggcatcca cgcgacgccc ttgccgacac gctcggtgat
     2281 gagcgccttt accttggcct tcgcgttgtt ttcggcgccg gtgacccaca cccagccgcc
     2341 gtccttgatg ccgcgggcgc tggcgtcggc aacattgatc tcgatgaaca tgtcttgctg
     2401 cagttcggcg agccacctgt tggagcgggt ctcttcgccg ccaccctcgt actcgaccaa
     2461 tcggccggtc gagaggatga gcgggaactg cttcgcgata cccttgtcga ccgccgcctt
     2521 ctgcaccgtg aagccgatat ttggcagccg gaactgcttg gcgtcgggca aggtcgggta
     2581 cttctgaatc agctcgacgc gcggcgaata aatcggttcg cgatgcaccg ggatcggatc
     2641 gggcaagttc caggcattca tgcgcgcctt gccgtttccg taggggatac agccgtgttt
     2701 caacgccacg cgctgcacac cgccggagag gtcggtcgac caggacaccg cgtccggatt
     2761 ggcgggattg accttgttga tgacggccag ttcggctgcg gtcagatcct tgtcccagcc
     2821 gagctttttg agcacgccaa ccgtaaactg cggatacccg tccttgatct cggaatcctt
     2881 ggagaaagag ccatcggcca gcatgctgac tttcactgtg gtgccgtcc
//
LOCUS       DASSRL010000191         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1579847, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000191 DASSRL010000000
VERSION     DASSRL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579847"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            555..791
                     /locus_tag="VFL49_04015"
     CDS             555..791
                     /locus_tag="VFL49_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911169.1"
                     /translation="MNKAVNVLGWIIFGAIGFSLGMAGNPMGWVFVLAAAWAIIATIR
                     GQADAKDGQAGSSNSWFDIGGCGGCSGGCGGCGD"
     gene            1367..1582
                     /locus_tag="VFL49_04020"
     CDS             1367..1582
                     /locus_tag="VFL49_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911170.1"
                     /translation="MKTAVSLTFWTIFGAIALTVAFTGHAVGWIGVVMAVFGIKQALW
                     PSKKSADSISSFDIDAGGCGGCGGCGG"
     gene            1843..2397
                     /locus_tag="VFL49_04025"
     CDS             1843..2397
                     /locus_tag="VFL49_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911171.1"
                     /translation="MPAGDAGGAATIEGFSRPPTFTAPAGCIGDEKAYDVMAHCTKSL
                     EPGHEFTVRIDTSVGVGYVDSAEFVLDRVEVARQWWRENFSWKASFSSKASDIVPSNA
                     PSHGTICMEYSVMAKTEKEIQHVEGLACAWPVEKPKAGKWTVESFWLEANDTYGASQQ
                     PMASFDAVVRDLLLSARLTPSQRQ"
BASE COUNT          498 a          711 c          848 g          484 t
ORIGIN      
        1 cgcctcggtg acgatgttgc ttttcttgag cgacaggcct tcgtcggccc ggcgggcgag
       61 aaccgcaagc gctgcatcgt acgcgaagat ccctgccgcc gccgtggctg ccagaacggc
      121 cgcggcagtc agaagcagca aaacggtgcg taaccgcatg atgcgcatac gcacgatctc
      181 tgatcctgca agctgcagtc cgggtgtgga ctgaacgccg agagtcaccg acgtgactga
      241 accacaacca aatataggcg tgaatggatc tagggtaaag cgtccgccgc tgcaagcctg
      301 gccgcgagcg cacgttcaat cgggctttga attcatgtcg ctgcggggcc ggacaaatcc
      361 ggcgaactct cgctgatcga tcggcgagct ctcgccggcc gggaacttct cgcattgggg
      421 agcggaagaa tttcatgcgg ctggtcaatc ggttggcggc aggcgtcggt cgtcgcccaa
      481 gtggcatgcg ccttgcggaa gcaggtgcgc gaaacctcac ggagcaagcc gcttggcggc
      541 gaggatgcgg atcgatgaac aaggctgtga atgttctagg gtggatcatc ttcggcgcta
      601 tcgggttctc gctcggcatg gcgggcaatc cgatgggatg ggtcttcgtg ctggctgctg
      661 cctgggcgat catcgccacg attcgtgggc aggccgatgc gaaggatggc caggctggta
      721 gcagcaacag ctggttcgac attggtggtt gcggcggctg cagcggcggc tgcggcggat
      781 gtggcgacta gggggcgacg cagtccccag catagcgacg tctccgcgcg agccgcgcat
      841 gtccggctgg ccggaatccg acaatcgctc tgatcgaatt gacggcagct tgacagcacc
      901 ttgcacgcct gcggacaggg cccggacggc cttggactat ggtgcttcct cgatgccacc
      961 gaaggaggca gcgatgattg aaatcctgga acggctgagc gagatcgcgc gccgttgcgg
     1021 agacgggaag cctctggacg cagacgaggc gttgtggttc cggcactgtt ccgccgtgaa
     1081 gcgttccagc atagcatggt ttggcccctc tcaggagatc tggcgtccga tcacgcgcga
     1141 cggcaatccg aagcgggtcg gacgcccaac agtgcgccgg ctcacggctc atcggcgaga
     1201 gtccgccgcc ctatcggtgg attcttgcgc ccacattgcc gcacctcccg agaaccatgt
     1261 gcatttccaa gcaattccag gcagtggcgt gatgaaagcg agttggcaca cgccttgcgc
     1321 tgagcgggtt ggccatcaag agcgagcgat gggaaggagc cctgcaatga agaccgctgt
     1381 ttccttgacc ttctggacga tcttcggcgc cattgcgctg acggtcgcct ttaccggtca
     1441 tgccgttgga tggatcgggg tcgtcatggc cgtctttgga atcaagcagg cgctgtggcc
     1501 gtcaaagaag agcgcggatt cgatcagctc gttcgacatc gatgccggcg gctgcggcgg
     1561 ttgcggcggt tgtggcggtt gagggagact gccatgctgg acgctggcca ggccggtcgt
     1621 cgcgagatcc cgatcagtga gtccgagaca tgtcattgtt gggcaaacgt tgtcgcacgg
     1681 catctcacat caagcactgc gaagcaaatc actgcgccag ttggcgagag atggacgcgc
     1741 agcgacgacg atgtttccgt atccatcatc ccggagaatt gacacatggc aaagaaaccg
     1801 ttgtcactct tggcatggcc actcgggctc gcagcgcttc tcatgccggc gggggacgcc
     1861 ggtggcgccg ccaccatcga gggattcagc cgcccaccaa cattcacggc acccgccggc
     1921 tgcattggcg atgaaaaagc ctatgacgtc atggctcact gcacgaaatc gctcgagccg
     1981 gggcatgaat tcacggtgcg catcgataca tcagtcggtg tggggtatgt ggacagcgca
     2041 gaattcgtgc tcgatcgagt tgaagtggca aggcaatggt ggagagagaa tttttcgtgg
     2101 aaggccagtt tttcctccaa ggcctcggat attgtgccca gcaacgcgcc gtcgcacgga
     2161 acgatttgca tggaatactc ggtcatggcg aagaccgaaa aagagattca gcacgtggag
     2221 ggcctggcct gcgcctggcc ggtcgaaaaa ccgaaggcgg ggaaatggac tgtcgagagt
     2281 ttctggctgg aggccaacga cacgtacggg gcatcgcagc aacccatggc gtcattcgac
     2341 gccgttgtgc gcgatttgtt gttgtccgcg cgcctgacgc cgtcacagcg gcagtagcct
     2401 cacttgggct cttgttctcg ttgaggttca tgggcggctt cgtcttcagg atcgcgccgg
     2461 ggagcaaaat atgacgagcg agaagataga gaccgtaatc attggccgtg aattggacag
     2521 tagggccgcg ggccgcgaac g
//
LOCUS       DASSRL010000192         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1667405, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000192 DASSRL010000000
VERSION     DASSRL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667405"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFL49_04030"
     CDS             <1..183
                     /locus_tag="VFL49_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HET7911172.1"
                     /translation="EEEKKATSDDIRDVYAEAKGTGFDTKALRTIVRLRKLDTDERRE
                     QQEVLDTYLHAMGMAN"
     gene            complement(188..1708)
                     /locus_tag="VFL49_04035"
     CDS             complement(188..1708)
                     /locus_tag="VFL49_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HET7911173.1"
                     /translation="MLGANDALQNASAEGDTRTLSFHHVHTGEDITITYKRNGRYDDA
                     ALKKLDWFMRDWRKEKSTRMDPHLFDLLWETYREVGATQPIDVVCGYRSPETNKMLRN
                     RSSGVAQNSNHITGHAMDFFIPGVSLAKIREVGLRLQRGGVGFYPTSGSPFVHLDTGT
                     VRHWPRMTHDQLVKVFPDGRTVHIPSDGHPLRGYALALADVERRGDMPSEMSLEAARE
                     HGAIGADATGKPKRSLFARLFGRGKDTDEVNEEPAPKSTRAPVAVASVSAPKAKPVSV
                     EHIVPLPTARPKAVAVAAAPTPLKQTAIASLGNKLFDTRGYWHGTVQNSPELPAAVAV
                     NTAYQTASAGPDATGSTALAYAAQSGVPALTRTRPMGSNTGASMESQLSPRQPEAKVM
                     PVQGNTTVVAKPSLSADALTGTRRDSPWLRAAMVTPSVSGYLTATRMGAVDMRPLHGL
                     LHKPSMSVVMRFSADPRLGLASDRFNGPAVVFVATTTFATPSTAAAFSPQTTASLR"
     gene            complement(2043..>3103)
                     /locus_tag="VFL49_04040"
     CDS             complement(2043..>3103)
                     /locus_tag="VFL49_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7911174.1"
                     /translation="SIGDVVTRSPAMRPVLKSAEKAAASAIPVLVEGESGVGKELIAR
                     AIHGSSDRRMKPFVAVNCGALPETLVESILFGHEKGAFTGATERHSGKFAEADGGTLF
                     LDEIGELPTPAQVKLLRALQDGEVEPVGGRRNVKVDVRIVSATNRDLIADVKAGRFRE
                     DLFYRLHVFPISVPPLRMRRDDIPELARHFLARIAAEEGKRIRAINADGMTLLAAYHW
                     PGNVRQLENSIFRAVVLADGEEIGADEFPQIAAHVSSGPAPPPLIEPSPAMTASWPEP
                     GGGSIEAAKPPGPTLPMTDARGDVRPLEEIEREAIRFAISHYRGQMSEVARRLKIGRS
                     TLYRKLEGLGLGEDVSAG"
BASE COUNT          563 a          958 c         1001 g          581 t
ORIGIN      
        1 gaggaagaga aaaaggccac ctccgacgat atccgtgacg tgtacgccga ggcgaagggc
       61 acgggcttcg acaccaaagc cttgcgcacg atcgtgcgcc ttcgcaagct ggacaccgat
      121 gagcgccgcg agcagcagga agtgctcgac acctatttgc acgctatggg gatggcgaac
      181 tagcggatca acggagcgat gcggtcgttt gcggcgagaa tgctgcagca gtcgaaggcg
      241 ttgcaaacgt cgtcgtggcg acaaaaacga ccgccgggcc gttgaagcgg tccgacgcca
      301 ggccaagccg aggatcggcc gagaatctca taacgaccga catcgacggc ttgtgcagca
      361 acccgtgcag cggccgcata tcgaccgcgc ccatgcgtgt cgcggtcagg tacccgctga
      421 cgctcggcgt gaccatcgcg gcgcgcagcc atggactgtc acgtcgcgta ccggtcaaag
      481 cgtcggctga aagtgacggt ttggccacga ccgtcgtgtt gccttggacc ggcatgacct
      541 tggcttccgg ctgccgcggc gacaattgcg attccatgct cgcacccgtg ttcgaaccca
      601 tcggtcgggt gcgcgtcaac gcaggcacgc cggattgcgc cgcgtaagcg agcgcggtgc
      661 tgccggtggc gtcgggcccc gcgctggccg tctgataggc cgtgtttaca gcgactgctg
      721 ccggcaattc agggctgttc tgcacggtac cgtgccagta tccgcgggtg tcaaacagct
      781 tgttgccgag cgaagcgata gctgtctgct tcagaggtgt cggtgccgca gcgacagcga
      841 cggctttcgg ccgtgccgtg ggcagcggga cgatgtgctc gacgctcacc ggcttcgcct
      901 tcggtgcgct tacgctggcg accgcgacgg gcgcccgcgt tgatttcggc gcgggctcct
      961 cgttcacctc gtcggtatcc ttgccacgtc cgaacagccg ggcgaagaga ctgcgtttcg
     1021 gttttcccgt tgcatcggcg ccgatcgcgc cgtgctcgcg cgccgcctcg agcgacatct
     1081 cggacggcat gtcgccgcgc cgttcgacat cggcaagggc gagtgcgtag ccgcgcaacg
     1141 gatgaccgtc cgacgggatg tgtaccgtgc ggccatcagg aaaaactttg accaattgat
     1201 cgtgagtcat gcgcggccag tgccggacgg tgcccgtatc gagatgtacg aagggcgagc
     1261 ccgaggtcgg ataaaatccg actccgccgc gctgcagccg caggccgacc tcgcggatct
     1321 tcgccagcga tacgccaggg atgaagaagt ccatggcgtg accggtgatg tggttgctgt
     1381 tctgtgcgac gccgctcgag cggttgcgca gcatcttgtt ggtttccggc gagcggtagc
     1441 cgcagacaac gtcgatcggc tgcgtggcgc cgacctcgcg ataggtctcc cacagcagat
     1501 cgaacagatg cgggtccatg cgtgtcgact tttccttgcg ccagtcgcgc atgaaccagt
     1561 cgagcttctt aagcgcggcg tcgtcgtagc ggccgttgcg cttgtaggtg atggtgatgt
     1621 cttcgccggt gtggacgtgg tggaaggaca gcgtgcgcgt gtcgccttcg gcgcttgcgt
     1681 tctgcaacgc gtcgttggcg ccgagcatga ataatcctgc gagacccaga cgggccccgg
     1741 cgcgcataag agtcactgcg agcggacgct cgcgcgacgc gtgaataggc acagcgttga
     1801 cccgtgaaaa gatccgtccc acgtcgccgt cacgtccccg tcgggccgcc gtaacggcag
     1861 ccccgtgagt gtttatcggg atcggttaat gagaaattag cgccggtccc ccgtccggct
     1921 tctaactatc cgcggaaatg ggccgaaaac ggggcagtcc ggaacctggt ccctgatatt
     1981 tgccccggcc caccgcatcc ggattcttca aaaaaactgc aaataatgcg cgcggagcct
     2041 tgttatccgg ctgatacgtc ctcacccaaa ccgaggccct ccagtttgcg atagagggtc
     2101 gaccgcccga ttttaagccg acgtgccacc tccgacatct gtccgcggta atgggagatg
     2161 gcgaagcgga tggcttcacg ttctatttcc tcaagcggac gaacgtcgcc gcgggcatcg
     2221 gtcatcggca gcgtgggacc ggggggcttt gcggcctcga tcgagcctcc gcccggttcc
     2281 ggccaagatg cggtcattgc cggtgatggt tcgatcagcg gcggcggagc cgggccggat
     2341 gaaacatgcg cggcgatctg cggaaactca tcggcgccga tctcttcgcc gtccgcgagc
     2401 accacggccc tgaaaattga attctcaagc tgacgcacgt tgccgggcca atgataggcc
     2461 gcgagcagag tcataccgtc ggcattgatg gcgcggatgc gcttgccctc ttcggcggca
     2521 atgcgggcca ggaaatgacg cgccagttcg ggtatatcgt cgcgacgcat gcgaagcggc
     2581 ggcactgaaa ttggaaatac gtgcagacgg tagaacagat cttcacggaa acgcccagct
     2641 ttgacgtctg cgatcagatc gcgattggtc gccgaaacga tacgcacatc cacttttacg
     2701 ttcctgcgcc cgccgaccgg ctcgacttcg ccgtcctgca gggcccgcaa tagcttcact
     2761 tgcgccggcg tcggcaattc gccgatctca tcgagaaaca aagtgccgcc gtcggcttcc
     2821 gcgaattttc cagagtgtct ctcggtcgcg cccgtaaagg cacctttctc gtggccgaac
     2881 aaaatcgatt caacgagggt ttccggcagg gctccgcaat tgacggcgac gaacggcttc
     2941 atgcgccggt cgctgctgcc gtgaatggct cgtgcgatca gctccttgcc aactccggac
     3001 tcgccttcga ccagaacagg gatcgcggat gcagcggctt tttccgcaga cttgagcacg
     3061 ggtcgcattg cgggtgagcg cgtgaccacg tcgccgatgc tga
//
LOCUS       DASSRL010000193         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1702550, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000193 DASSRL010000000
VERSION     DASSRL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702550"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFL49_04045"
     CDS             complement(<1..345)
                     /locus_tag="VFL49_04045"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7911175.1"
                     /translation="MAQLSSQEWSPSASHAVKAERRNLTVVFCDMVGSTELSRRLDPE
                     DLRDIMRAFQRSCESAIFGFDGRIGRYMGDGVLAYFGFPAAHEDSAERAVQAALECVR
                     LVSELSFEGVPKI"
     gene            complement(408..2351)
                     /locus_tag="VFL49_04050"
     CDS             complement(408..2351)
                     /locus_tag="VFL49_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET7911176.1"
                     /translation="MSASDLDNEYDFIVVGSGAGGGPVAARIAEAGYSVLVLEAGGND
                     EPVDYKVPAFHSMSTEHPALAWKFYVHHHPDETQGRRDKYNFLSDEVVDGAPRNGIFY
                     PRAGTLGGCTAHHAMIFIAPHNSDWNYIAKLTGDKSWRPWRMRKYFQKLERCGYLNRP
                     WFRRVNFGRHGFGGWLPTTIADPALLLRDSQLLRLVVAAVQTCFTSDVWYSWGFRHRL
                     KAAVESFSDPTGLQRVGVMHRALSWLSGLLDPNSWLHLRAGSEGPIFVPLTVKDGVRA
                     GTRELIRDAERRMPRKLTVKLNALATRVLLEGNRAVGIEFLDGKHLYRADPAADRQGP
                     ITAPLRQVRARREVILAGGTFNTPQLLMLSGIGDPKELARHKIKINVALRGVGRNLQD
                     RLEVGVVHRMHEDFAILKHARLQPDDTEFADWRNGHGLYATNGVVAAMVKRSNLGQPD
                     PDLFVFAIPGHFSGYRPRYSEWGREKNYFSWIILKGHTKGHTGRVQLETDDPRDPPSI
                     NLNYFDPATPDVDEDAHAVAAGIEFVRKMSGRAASLMTEIIPGLQTRTRPQLTQFVRD
                     CAWGHHACGTCKIGADDDGDAVLDGRFKVRDTQGLRVVDASIFPKIPGLFILSAIYMA
                     AEKAADVIIADAREIDRKRKMIT"
     gene            complement(2464..>3363)
                     /locus_tag="VFL49_04055"
     CDS             complement(2464..>3363)
                     /locus_tag="VFL49_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911177.1"
                     /translation="RGSYWGRFVERTDGFAIRPASFKFRNDAGHEVEFTAPYAVKKIK
                     RLQPLALDEYMFLRSVTKLTGKITLPAPSTMHFYRCNDFADRAAYSDVDSFFADLAKV
                     FRQEITDLARAGCRYVQLDEVAVALLCDPAIRDEITRSGSEPDALVDLYIKSINDAVA
                     ECPPDVTVGVHMCRGNFKGHYLGAGGYESVAKRFFAGTRVNHFLLEYDTPRAGDFAPL
                     RFVPKDKGVVLGVISSKTPALESLDALKRRLDEAARYIDLDRLAIGPQCGFASTVAGN
                     PVTEDDERRKLALVVEAARRFWP"
BASE COUNT          626 a         1052 c         1047 g          638 t
ORIGIN      
        1 gattttcggc acgccttcga atgacagttc cgaaacgagc ctgacgcatt ccagcgcggc
       61 ctggacggcc cgttcggcgc tgtcctcgtg cgcggctgga aatccgaaat aggcgaggac
      121 gccgtcaccc atatagcggc cgatccggcc gtcaaatccg aaaattgcgc tttcacagga
      181 tcgctggaac gcgcgcatga tgtcgcgcag gtcctcgggg tcgagacgac gcgacagctc
      241 ggtcgagccg accatgtcgc agaagacaac cgtcagattc cgccgctccg ccttgacggc
      301 gtgtgacgca gacggcgacc actcttgact cgacaactgc gccacgttcg cccctttcgc
      361 gagttgtccg aacaaaagtt ttatcgctac tttatatcat tgtttcgcta ggtgatcatc
      421 ttgcgctttc tgtcgatttc ccgggcatcg gcgatgatca cgtcggccgc cttctcggcg
      481 gccatgtaga ttgcgctcag aataaacagg cctgggattt tcggaaaaat cgacgcatcg
      541 acgacacgca atccctgcgt gtcccgcact ttgaagcggc cgtcgagcac ggcgtcgcca
      601 tcgtcgtcgg cgccgatctt gcacgtgccg caggcgtgat gaccccacgc gcagtccctc
      661 acgaattgag tcagttgggg gcgcgtgcgg gtctgcaggc ccgggatgat ctcggtcatg
      721 agactcgcgg cacgcccgct catcttgcgg acgaattcga taccggcagc gaccgcatgg
      781 gcatcttcat cgacgtcagg cgtcgccgga tcgaaataat tgagattgat ggacggtgga
      841 tcgcgcggat cgtctgtttc cagctgcacg cggccggtgt ggcctttggt atggcccttc
      901 aggatgatcc agctgaaata attcttttcc cgtccccatt cggaataacg ggggcggtag
      961 ccggaaaagt gtccgggaat tgcgaagacg aacaggtccg ggtccggctg cccgagattg
     1021 gatcgcttga ccatcgccgc gacgacgccg ttggtggcgt aaaggccatg gccgttgcgc
     1081 cagtcggcga attccgtgtc gtccggctgc agcctggcat gcttgaggat ggcaaagtcc
     1141 tcgtgcatcc ggtgcacgac gccgacctcg agacgatcct gcaggtttct gccgacgccg
     1201 cgcagcgcga cattgatctt gatcttgtgc ctggccagct ccttcggatc gccgatcccc
     1261 gacagcatca gcagctgcgg cgtgttgaaa gtccctccgg cgaggatcac ttcgcgccgc
     1321 gcacgcacct gccgcagcgg cgcggtgatg ggtccctgcc tgtccgcagc cggatcggcg
     1381 cgataaagat gttttccatc aagaaattcg atcccgacgg cgcggttgcc ctccagcagg
     1441 acccgtgtcg cgagggcatt caactttacg gtcagcttcc tgggcatccg cctttccgcg
     1501 tcgcggatga gctcgcgtgt tcccgcgcgc acgccgtcct tcaccgtcaa tggcacgaag
     1561 atcggtcctt cgctgccggc acgcagatgc agccagctgt tcgggtcgag caggcccgac
     1621 agccaggaca gtgcgcgatg catgacgccg acgcgctgca ggccggtcgg atcgctgaag
     1681 ctttccactg cagccttcag gcggtgccgg aagccccacg aataccagac gtcggacgta
     1741 aagcacgtct gcacggcagc cacgaccagc cgcagcagtt gcgagtcgcg cagcagcagt
     1801 gccgggtccg caatcgtggt cggcagccag ccgccgaacc cgtgccgccc gaaattcacc
     1861 cttctgaacc acggacggtt cagatatccg cagcgctcga gcttctggaa gtacttgcgc
     1921 atgcgccagg ggcgccagct tttgtcgccg gtcagcttcg cgatgtaatt ccagtcgctg
     1981 ttgtgaggcg cgatgaaaat catcgcgtgg tgcgcggtgc agccgcccag cgtgccggcg
     2041 cgcggataga agattccgtt gcgcggcgcg ccgtcgacca cctcgtcgct caggaagttg
     2101 tatttgtccc ggcggccctg agtctcgtca ggatggtgat gaacgtagaa tttccaggcg
     2161 agcgccggat gctcggtgct catcgaatga aatgcgggga ccttgtaatc gacgggctcg
     2221 tcgttgccgc cggcctcgag aacgagaacg ctgtagccgg cttccgcgat ccgcgcggcg
     2281 accggcccgc cgccggcgcc ggatcctacg acaatgaaat cgtattcatt atccaaatcg
     2341 cttgcggaca tcgcgggcct cgcccgaacg agactataga tgaccgcaaa cctttgcgta
     2401 agcgcaccgg gcaaacaccg ctatttgcga tgaacgaaac accccgaaaa taccgacgtt
     2461 tgttcacggc cagaaccggc gcgccgcttc gacaaccagc gccagcttcc ggcgctcgtc
     2521 gtcttcggtg accggattgc cggcgacagt gctggcaaag ccgcattgcg gaccgatggc
     2581 aagccggtcg agatcaatgt aacgcgccgc ttcgtcgagg cggcgcttga gcgcgtcgag
     2641 gctttcaagc gccggcgtct tcgagctgat cacgcccaac accacgccct tgtccttcgg
     2701 tacgaaacgc agcggtgcga aatcgccggc gcgcggcgtg tcgtattcga gcaggaaatg
     2761 gttcaccctg gtgccggcga agaaccgctt ggcgaccgac tcgtagccgc cggcgccgag
     2821 atagtggccc ttgaagttgc cgcggcacat gtgcacgccg accgtcacgt cgggcggaca
     2881 ctccgcaacg gcatcgttga tgctcttgat gtaaagatcg acgagcgcgt cgggctcgct
     2941 gcccgatcgc gtgatctcgt cgcgaatggc gggatcgcac agaagcgcga cggcgacttc
     3001 atcgagctgc acgtaacggc agccggcccg ggcgagatcg gtgatttcct gccggaatac
     3061 tttcgccagg tcggcgaaaa atgaatcgac atcggaatac gccgcgcggt cggcaaagtc
     3121 gttgcagcgg tagaaatgca tggtcgaggg tgcgggaagg gtgatcttgc cggtaagttt
     3181 cgtcacgctg cgcaggaaca tgtactcgtc gagcgccagc ggctgcagcc gtttgatctt
     3241 tttcacggcg taaggcgccg taaactcgac ctcgtggccg gcatcgttgc ggaacttgaa
     3301 cgaggcgggc ctgatcgcaa aaccgtcggt gcgctcgacg aagcgtcccc agtacgagcc
     3361 gcg
//
LOCUS       DASSRL010000194         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_5348, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000194 DASSRL010000000
VERSION     DASSRL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5348"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VFL49_04060"
     CDS             complement(<1..142)
                     /locus_tag="VFL49_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7911178.1"
                     /translation="MPHITARDGTRLYYEEAGQGTPVVFVHEYAADYRTWEPQMRRFS
                     RSH"
     gene            265..1251
                     /locus_tag="VFL49_04065"
     CDS             265..1251
                     /locus_tag="VFL49_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911179.1"
                     /translation="MAKISRRGFAAVFACAAALTVNFFAPNAQAQQKPVIKVSSLTLP
                     VFNPLVWNIMKARGIDAKHGFELDAKAYPSISAFYAAFATGETDVLIGGPTILQKLYQ
                     EGVPVRIIATGFTLADLVIFARDEKIKSLADLKGKQLAIDMGGSQFQVTKIYANAKGI
                     DLGKDIITVNANFAVARAQLEAGRVDAALVIEPLASITAKQHPDWHVIFNGAQGWKEI
                     TGGDGWEIVTAMRADAIAKNPKAPQMLLAALQDVADVMHKDTAAGDKIANETLKLPPG
                     ILTAAVESKRLQMIIKPAWDPATRKSITDIMERAVKAGFYEKMPDKKIIYSP"
     gene            1272..2042
                     /locus_tag="VFL49_04070"
     CDS             1272..2042
                     /locus_tag="VFL49_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7911180.1"
                     /translation="MSTEHRESVPVLIFPRQTLVSLLVFALAWEVLSHLAPALGIPPF
                     AIPSLLRIAKAITTITPIDIAVTLARVIGALIASFVLGIFLAMAMYRSDSLDKYLHPM
                     IRILMAVPVVSWILFAVLWFPGVEFRIGFVLVVVCGPVFLVDTLDAMRSVPRELRQMM
                     RSFRPTTLQFFVKLMLPAIVPTIFTSWKVNISLAIRVVTIAELVGAVTGIGHQLSVAQ
                     ELFSVADVFAWTIVLVALLFLLEAIVSRVETRLLRWRA"
     gene            2039..>2481
                     /locus_tag="VFL49_04075"
     CDS             2039..>2481
                     /locus_tag="VFL49_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7911181.1"
                     /translation="MIGTMNGPPPINVAGVRKVFRQATTGQSVVAIDRLDLTIAPREV
                     VAIVGQTGCGKSTFFDLMIGLEAPTEGTIHIGDRSPHADFDYFRGRIATVFQQDRLLP
                     WRSALDNARLPLELIGVPEAEQRERSLTWLNRLGLGQFTNAYPHE"
BASE COUNT          465 a          786 c          752 g          478 t
ORIGIN      
        1 ggtgcgagcg cgagaaacgc cgcatctgtg gctcccaggt gcggtagtct gccgcatact
       61 catgcacaaa gacgaccggc gtgccctgcc ccgcctcttc gtagtaaagc cgcgtcccgt
      121 ctcgcgcggt gatatgcggc atcacgccct ccactcgcca gtggtcaatt atttgcacgc
      181 aaacgaaagc ggagtaaagt cgcgctcgcc ttcgagcaag ctgacgcgca agatcgctgc
      241 ccgaaatatg caagggggaa cgtcatggcc aagatatctc gacgggggtt cgccgcggta
      301 ttcgcgtgcg cggcggcact gacagtcaat ttcttcgcgc cgaacgcgca ggcgcagcaa
      361 aagccggtca tcaaagtatc cagcctcacc ttgccggtgt tcaacccact cgtgtggaac
      421 atcatgaaag cacgcggcat tgacgccaag cacggctttg agctcgacgc gaaagcctat
      481 ccatcgatct ccgcgttcta tgcggctttc gcgacgggcg agaccgacgt gctcatcggc
      541 ggccccacca tcctgcagaa gctctatcag gaaggtgtgc cggtgcgcat tatcgccacc
      601 ggcttcacgc tcgccgatct cgtgattttc gcaagggacg agaagatcaa gtcgctcgcc
      661 gatctcaaag gcaagcagct cgccatcgac atgggcggat cgcaattcca ggtgacgaag
      721 atctacgcga acgccaaggg catcgatctc ggcaaggaca tcattacggt gaatgcgaac
      781 ttcgcggttg cgcgcgcgca actcgaggcc ggccgcgttg acgcagcgct tgtcatcgaa
      841 ccgctcgcca gcatcaccgc caagcaacat cccgactggc acgtcatctt caacggcgcg
      901 cagggctgga aggaaatcac cggcggcgac ggctgggaga tcgtgaccgc catgcgtgcc
      961 gacgcgatcg caaagaatcc gaaggcgccg cagatgctgc tagcggccct gcaggacgtt
     1021 gcggacgtca tgcataagga cacggccgcc ggcgacaaga tcgccaacga gacgctcaag
     1081 ctgccgccgg gaattctcac cgccgcagtc gagagcaagc ggctgcaaat gatcatcaag
     1141 ccggcctggg atccggcaac gcgcaagtcc atcaccgata tcatggaacg cgcggtgaag
     1201 gccggatttt acgagaagat gccggacaag aagatcatct atagcccgtg agcggatagt
     1261 cgcaccttac gatgagcacg gagcatcgcg agtctgtccc ggtcctgatt tttccgcgtc
     1321 agactctcgt ctccctgctc gtcttcgcgc tggcatggga agtgctgtcg catctggcgc
     1381 cggcgctggg gataccgccg ttcgcaattc cgagcctgct gcggatcgcg aaggcgatta
     1441 cgactattac acccatcgac atcgcggtca ctctggcgcg cgttatcggc gcgctcattg
     1501 cctcgttcgt gctcgggatt ttcctggcga tggcaatgta tcgctccgat tcgttggaca
     1561 aatacctgca cccgatgatc cgcatcctga tggcggtgcc ggtcgtgtcg tggattctgt
     1621 tcgcggtgct gtggtttccc ggcgtcgaat tccgcatcgg gttcgtgctg gtcgtcgtct
     1681 gcggtccggt tttcctggtc gacacgctcg atgccatgcg ctcggtgccg cgcgagctgc
     1741 ggcagatgat gcgctcgttt cgcccgacca ccttgcagtt tttcgtgaag ctgatgctgc
     1801 cggcgatcgt gccgacgatc ttcaccagct ggaaggtcaa tatcagcctg gcgatccgcg
     1861 tggtgaccat cgccgagctg gtcggcgccg tgaccggcat cgggcatcag ctgtccgtcg
     1921 cgcaggaact gttctcggtc gccgacgtct tcgcctggac catcgtgctg gtcgcgctgc
     1981 tgttcctgct cgaggccatc gtgtcacgag tcgaaacgcg cctgttgcgg tggcgcgcat
     2041 gatcgggacc atgaatgggc ccccgccgat caatgtcgcc ggcgtgcgca aggtttttag
     2101 gcaggcgacg accggacaat ccgtcgtcgc catcgaccgg ctcgatctca ccatagcgcc
     2161 gcgcgaagtc gtggcgatcg tcgggcagac gggctgcggg aagtcgacct ttttcgacct
     2221 gatgatcggg ctggaggcgc cgaccgaggg caccattcat atcggcgaca ggagcccaca
     2281 cgccgatttc gactattttc gcggccgcat cgccactgtc ttccagcagg atcggctctt
     2341 gccatggcgt tccgcgctcg acaatgcccg gctgccgctc gaactcatcg gcgtaccgga
     2401 agctgaacag cgcgagcgca gcctgacgtg gctgaaccgg ctcggcctcg gccagttcac
     2461 caacgcctat ccgcacgaat t
//
LOCUS       DASSRL010000195         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_5397, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000195 DASSRL010000000
VERSION     DASSRL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5397"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VFL49_04080"
     CDS             <1..141
                     /locus_tag="VFL49_04080"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET7911182.1"
                     /translation="RYLVASGEKSDRLSVYKIDQGTGKLGEPNRFPVGKGANWVEIVD
                     VQ"
     gene            complement(184..2538)
                     /locus_tag="VFL49_04085"
     CDS             complement(184..2538)
                     /locus_tag="VFL49_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7911183.1"
                     /translation="MTEQPPKFRFIGQPIPRTEDARLLTGRGQFSDDFCFPGQTYAAI
                     VRSPHAHARIARIDASAAKKMPGVLGIFTGEDCLADRLAPIPHDPLPKTKFDMKLHGP
                     GGGEIFIGPHLLLPSDKVRHVGEAVAMVVAETQNRALDAAEAVNVSYEPLPCVTTAEE
                     AMQAGAPAVWDEVPDNILVDTRFGNAAETERAFGRAAHIVAKNFHVGRVTGAPMEPRA
                     AVAHYDATEGRYTIYAGSGGAVRQKRELAAVLGVDPEKIRVLSYDVGGNFGTRNRVYI
                     EFGLVLWAARKLGRPVKYTATRSDCFVSDYQGRDLVAKFELALDERGRFLALRATNIS
                     NVGARCVSLSPLSKGAGLITGSYDIPVATLQAMAIFTNTVPTQAYRSSGRPEVTFAIE
                     RLIDIAARRLGVDRVVLRRRNLVRPKAMPYRNAVGMVYDSGRYEENMDHALRLADWDG
                     FKSRQRDARKRGKLLGRGLANYVESSIGAPKEQTRMTVSAGGISVVIGTQPAGQGHET
                     SFAQVVADLLTVPVDKVRILLGDTDVVKAGGGTHSGRSMRHAATVFSLASTELMAKAK
                     RAVAVILGTGADQVEFNDGRFSARDSNRSFDFLELAEEMTRHPMPGDLSGGLAVITDN
                     EMHEPVFPNGCAICEIEIDAESCSLQLVRYTSVDDVGRCINPLIVHGQTQGAVIQGVG
                     QALWEQCIVDPGSGQPLCGSFMDYGMPRSDRVPSLVTKIVEVLSPTNPLGIKAGGEGG
                     TTAAPAAIVSAVVDALAPYGVEDLAMPVTPYRIWQAIHGVRAMR"
BASE COUNT          522 a          862 c          900 g          539 t
ORIGIN      
        1 cgatatctcg tcgcctcggg tgagaagtct gaccggctgt cggtctacaa gatcgatcag
       61 ggcaccggaa agctcggcga accaaaccgc tttccggtcg gcaaaggcgc aaactgggtg
      121 gagatcgtcg atgtgcagtg accgaaaagg gttcgcatac agcggacgac accacgatct
      181 attttatcgc atcgcgcgta caccgtggat cgcttgccag attcggtaag gtgtcaccgg
      241 catagccagg tcctcaacac catagggcgc gagggcatcg acaacggcgc tgacgatggc
      301 cgcaggtgct gccgtcgtgc cgccttcgcc gcccgccttg atgccgagcg gatttgtcgg
      361 tgaaagcacc tcgacgatct tggtgacgag ggacggcacg cgatccgacc gcggcatgcc
      421 gtagtccatg aacgagccgc aaagcggctg cccggaaccg ggatcgacaa tacattgctc
      481 ccagagggcc tgtcccacgc cctggatgac cgctccttgc gtctgtccgt ggacgatgag
      541 cggattgatg caccggccga catcatcgac cgaggtgtag cggacaagct gcaggctaca
      601 gctttcggcg tcgatctcga tttcgcaaat cgcacagcca ttcgggaaaa cgggctcatg
      661 catctcgttg tcggtgatga cagcgagtcc gcccgagagg tcgccgggca ttggatggcg
      721 tgtcatttcc tcggcgagct cgagaaagtc gaagctgcga ttgctgtcgc gagccgaaaa
      781 ccgcccgtcg ttgaattcga cctggtccgc acccgtgccc aggatgaccg caactgcacg
      841 tttcgccttt gccatgagtt cggtggaggc aagcgaaaac acggtcgctg cgtggcgcat
      901 ggagcggccg gaatgggtgc cgcctccggc cttcaccacg tcggtgtccc cgagaaggat
      961 acgcacttta tcgaccggta ccgtgagcag gtcggcgacc acctgcgcga aactggtttc
     1021 gtgcccttgc ccggccggct gcgtgccgat gacgacactg atgccgccgg cggacaccgt
     1081 catgcgtgtc tgttctttcg gcgctccgat cgaggactcg acgtagttcg ccagcccgcg
     1141 cccgagaagc ttgccccttt tcctggcatc gcgctggcgc gatttgaaac cgtcccagtc
     1201 ggcgagacgc aaggcatgat ccatgttctc ttcgtaacgg ccgctgtcgt aaaccatgcc
     1261 gacggcattc cggtatggca ttgccttcgg ccgtaccaga ttgcgacggc gcagcacaac
     1321 gcgatcgacg ccgagccgcc tggcggcgat gtcgatcaat cgttcgatcg cgaaggtgac
     1381 ctctgggcgt ccggagctgc gataggcctg ggtcgggacg gtattggtaa aaatggccat
     1441 ggcctgcaat gtggccaccg gaatgtcgta gctgcctgtg atcaagcccg cgcccttgct
     1501 caaaggcgaa agcgaaacgc atcgggcgcc gacattgctg atgttggttg cgcgcagcgc
     1561 caggaaccgg ccacgctcgt cgagcgccag ctcgaatttc gccaccagat cgcggccctg
     1621 gtagtcgctg acgaaacaat ccgagcgcgt tgcggtgtat tttaccgggc gcccgagctt
     1681 gcgcgcggcc cacagcacga gtccgaattc aatataaacg cgattacgcg tgccgaaatt
     1741 gccgccgacg tcataggaca gcacgcggat tttctcgggg tcgacgccga gcaccgcggc
     1801 aagctcgcgt ttttgcctga ccgctccgcc gctgccggca taaatcgtgt agcggccttc
     1861 ggtcgcgtca tagtgtgcaa ccgcggcgcg cggctccatc ggcgcgcctg tcacccggcc
     1921 gacgtgaaag ttctttgcga caatgtgggc ggcgcgcccg aaagcccgtt ccgtctcggc
     1981 cgcattgccg aaccgcgtgt cgaccaggat gttgtccgga acctcatccc agacggcggg
     2041 agcgccggcc tgcatggctt cttcggcggt ggtgacgcat ggcaatggct catatgaaac
     2101 gtttacggcc tcggccgcat cgagcgcccg gttttgcgtt tctgcgacca ccatcgcgac
     2161 ggcttcgccg acatggcgca ccttgtcgga gggcaggagg aggtggggtc cgataaaaat
     2221 ctcgccacca ccgggcccat gaagcttcat gtcgaacttt gtctttggca gcgggtcatg
     2281 cgggatgggc gcgagcctgt cggccaggca gtcttcgccg gtgaagatac cgaggacgcc
     2341 cggcattttc ttggcggcgc tcgcgtcgat gcgcgcgatg cgcgcatgcg cgtgtggtga
     2401 acggacaatc gctgcgtagg tctgcccggg aaaacagaaa tcgtcgctga attgcccgcg
     2461 gcccgtgagc aggcgggcgt cttccgtgcg tgggatgggc tgcccgatga agcggaattt
     2521 tggaggttgc tcggtcatcg gttcgtgcag actccgctgc gggaaaggct gcagttgatc
     2581 gcaaaacgct tgcaaatcca accgcgttgc gtgcggcgga ggcttgcctc aaatgccttg
     2641 ctgggccgcc agccatggga aatactgcgg cccaaatcgc gcggaaaccg gatgaaacac
     2701 gtctcgataa aatcgtcgac tggccccctg atcgtcggca tcaccggcgc gtcgggcgtg
     2761 atctacggcg tcaggctgct cgagctgctg cgcgaatgga acattgaaac ccatttgatc
     2821 gtt
//
LOCUS       DASSRL010000196         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1352578, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000196 DASSRL010000000
VERSION     DASSRL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352578"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..582
                     /gene="mutT"
                     /locus_tag="VFL49_04090"
     CDS             178..582
                     /gene="mutT"
                     /locus_tag="VFL49_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HET7911184.1"
                     /translation="MATKLVLVAACALVDADGRVLIAQRPQGKPMAGLWEFPGGKVED
                     GERPEDTLIRELREELSITVNEACLAPLAFASHTYPDFHLLMPLYVCRRWDGTVTPTE
                     GQQLAWVKPNRLRDYEMPPADVPLISHLMTLL"
     gene            complement(579..1475)
                     /locus_tag="VFL49_04095"
     CDS             complement(579..1475)
                     /locus_tag="VFL49_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7911185.1"
                     /translation="MSSGPNIFDRRLLRVRQRRARLLTPATFLLDRVAADLGERLSAV
                     LRKFDVAMDLGTPSDAVRRVLATNENIGAVVAAGLADDDASLRVAVDEESLPFAEGSL
                     DLVVSALALQFVNDLPGTLIQIRRALRPDGLLLAAMIGGDTLTELRSSFAAAESEIEG
                     GVSPRVAPFADIRELGGLLQRAGFALPVVDSERLAVRYNSALALMRDLRRMGATNILH
                     ERLRKPLKRSTLERVTAIYADRFADPDGRIRATFEIIWMSGWAPHQSQQKPLKPGSAT
                     QRLADALGTTELPSGEKPERAR"
     gene            1542..>2289
                     /locus_tag="VFL49_04100"
     CDS             1542..>2289
                     /locus_tag="VFL49_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7911186.1"
                     /translation="MNELASARPAGATPVVARFRKAFRSALRLALDFALPQLCPSCRE
                     PVGDGAGLCAQCWAGLSLIEPPYCARLGIPFAYDPGPGLLSMEAIANPPAYDRARAAV
                     RYDDISRALVHSLKYGDRLDLSPMMGQWMARAGHELLADADGLVPVPLHWRRQWARRF
                     NQSAALAGVISAITGVPVIHGGLKRVRATPQQVGKSKAERADNVQGAFRTAAEHSADI
                     AGRRLVLIDDVLTSGATVDACARALLRASAA"
BASE COUNT          416 a          742 c          715 g          416 t
ORIGIN      
        1 agcaccaaac ccaacgcaat gctggaatac ctgatcatcc ccgccccccg gctgaagaac
       61 aactgaggat aggctgcaac ggaccattgc atcaatgacc tgtcacgctc attaacccgt
      121 cttgcagaca gttcattgga ctgtccttga ttggcgcttg aattccgtcg agggactgtg
      181 gccaccaaac ttgtccttgt cgccgcctgc gcgctcgtcg acgccgatgg ccgtgtgctg
      241 atcgcgcaaa gaccgcaagg caagccgatg gccggcctct gggaattccc tggcggcaag
      301 gtcgaggatg gcgagcggcc ggaagacaca ttgatacgcg aattgcgcga ggaactctcg
      361 ataacggtga acgaggcctg tctcgccccg ctcgcgtttg cgagccatac ctaccccgac
      421 ttccaccttc tgatgccgct ctacgtatgc cgccgctggg acggcaccgt cactccgacc
      481 gaaggtcagc agcttgcatg ggttaagccg aaccggctgc gcgactacga aatgccgccg
      541 gcagacgtgc cgctcatttc gcacctaatg acgttgctct aacgagcacg ctccggtttt
      601 tccccggacg gcaattccgt ggtgccaagt gcgtcggcca ggcgctgcgt tgccgagcct
      661 ggcttgagcg gcttctgctg gctttggtgc ggcgcccagc cggacatcca gatgatttcg
      721 aacgtcgcac ggatgcgccc atccggatcg gcgaagcgat cggcatagat tgccgtcacg
      781 cgttccagcg tgctgcgttt gagaggcttg cgaagccgct cgtgcagaat gttggtcgcg
      841 cccatgcgcc ggaggtcgcg catcagcgcg agcgccgaat tgtagcgtac tgcaagccgt
      901 tcgctgtcga cgaccggcaa cgcaaagccg gcgcgctgta aaagaccgcc aagctcgcgg
      961 atgtcggcga agggtgcgac gcgcggcgag acaccgccct cgatctcact ctcggcggcg
     1021 gcaaatgacg accgcagctc ggtcagggta tctccgccga tcatggccgc cagcaacaat
     1081 ccgtccggcc tcagcgcacg gcggatctga atcagcgttc cgggcagatc attgacgaat
     1141 tgcaacgcca gcgcggacac gacgagatcg agagagcctt cggcaaaggg caaggactcc
     1201 tcgtcgacgg caacacgcag acttgcatca tcgtctgcca gtccggctgc aacgacggcg
     1261 ccgatatttt catttgtcgc cagcacgcgg cgcacggcat cggatggcgt gccaagatcc
     1321 atagccacat cgaacttgcg caagaccgcg gaaagccgct cgcccagatc ggcagcgacg
     1381 cgatccagca gaaatgtcgc aggtgtcagc aatctcgcgc gtctttggcg tacgcgaagc
     1441 aaccgccgat caaagatatt ggggcctgac gacattgcat tgtcctatgg cgtcacgcgc
     1501 tgtcatgtca tgtccgcaga cgatcatgct actgttgcag catgaacgag ctcgcttcgg
     1561 caagaccggc tggcgcaacg ccagttgtcg cgcgctttcg taaggcattt cggtcggcgc
     1621 tgcgactcgc gctcgatttc gccctgcccc agctgtgtcc gtcgtgccgc gagccggttg
     1681 gcgatggagc cggcctttgc gcgcagtgct gggccggcct ttcgctcatc gagccgccct
     1741 actgcgcacg cctggggatt ccgttcgcct atgatcccgg cccgggcctg ctgtcgatgg
     1801 aagcgattgc caacccgccg gcctatgatc gcgcccgcgc cgccgtgcgc tacgacgaca
     1861 tttcgcgcgc gcttgtccat agcctcaaat acggagaccg cctcgacctc tcgccaatga
     1921 tgggccaatg gatggcgcgc gcggggcacg aactgctggc ggatgccgac ggactcgtgc
     1981 cggtgccgct gcattggcgg cggcagtggg cgcggcgttt caaccagtcg gcggcgcttg
     2041 ccggtgttat ctcggcaata accggcgtgc ccgtcatcca tggcggtctc aaacgcgtac
     2101 gggcaacgcc ccagcaggtg ggcaagtcga aggcggagcg cgccgacaat gtgcagggcg
     2161 ccttccggac cgccgcggag catagcgcgg atattgccgg acggcggctg gtgctgatcg
     2221 acgatgtgct tacttcgggc gcaacggtcg acgcctgcgc tcgcgcttta ttgcgggcga
     2281 gcgccgccg
//
LOCUS       DASSRL010000197         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1317687, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000197 DASSRL010000000
VERSION     DASSRL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317687"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFL49_04105"
     CDS             complement(<1..432)
                     /locus_tag="VFL49_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7911187.1"
                     /translation="MTVAVRSPARKKVLPPAAAELLAWYERHARVRPWRAGPRRRTDA
                     YRVWLSEIMLQQTTVKAVAPYYARFLSQWPTIEGLAGAELEDVLRAWAGLGYYARARN
                     LHSCAKVVVERYGAQFPEDIDALRTLPGIGDYTAAAIAAIAF"
     gene            463..936
                     /locus_tag="VFL49_04110"
     CDS             463..936
                     /locus_tag="VFL49_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HET7911188.1"
                     /translation="MSKPSHSFPRALSQLLNATLSDVLKAQGFASTEIISRWSDIVGP
                     EIAGHSEPLRINWPRAAVEKAEPATLVLRVEGPAALEIQHLSAVILERVNRFFGWQAI
                     GRIAIRQAPLRRREPSPLPKIDNGAAERIADALPDVKDENLRQALGRLGAAIKGN"
     gene            1037..1705
                     /locus_tag="VFL49_04115"
     CDS             1037..1705
                     /locus_tag="VFL49_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET7911189.1"
                     /translation="MPMTRREFCQTTATLALATSMLSFSSLSPFPGVAFAETVPTDEL
                     MKPGALPDMAMGDAKAPVTIIEYASMTCPHCAHFQEVTFPELKKRYIDTGKVRYIFRE
                     FPLDSLAAAAFMLARCAGKDDKDKYFALIDTLFRQQRQWAVEKPIPPLLAIAKQAGFT
                     EQTFNACLANQQMLDGIENIRQRAIKQFKVQSTPTFFINGTAHPGALSIDEMAKLIDP
                     YLKG"
     gene            2059..>3031
                     /locus_tag="VFL49_04120"
     CDS             2059..>3031
                     /locus_tag="VFL49_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7911190.1"
                     /translation="MKLTRLRLLGFKSFVEPTDFMIEPGLTGVVGPNGCGKSNLVEAL
                     RWVMGESSYKSMRASSMDDVIFSGSNTRPARNNAEVAIAIDNSSRSAPAAFNDQDSLE
                     IARRIERDSGSNYRINGREVRARDVQILFADASTGARSPALVHQGRIGEIIQAKPEQR
                     RRVLEEAAGVAGLHARRHEAELRLKAAETNLLRVEDVIGQLSAQIDGLKKQARQAIRY
                     RTVSSQVRKAEATLYHLRWTAANNEVAEAEHAKDVSVRTVADRVAAEADASKRQALAA
                     AALPELREAEARAGAALHRLNVALQDIDREEGRAKDRIAELDLRLVQF"
BASE COUNT          601 a          969 c          921 g          540 t
ORIGIN      
        1 gaacgcaatc gccgcaatgg ccgcagcggt gtaatcgccg atacccggca gcgtgcgcag
       61 cgcgtcgatg tcttcgggaa attgtgcgcc ataccgctcg accacgacct tggcgcaact
      121 gtgcaggttg cgggcgcggg cgtagtagcc aagccccgcc caagcccgta agacatcttc
      181 gagttctgcc cccgccagcc cttcgatcgt cggccattgc gacaggaacc gtgcgtaata
      241 gggcgcgacc gctttgacgg tcgtctgctg cagcatgatt tcggacagcc acacacggta
      301 ggcatccgtc cggcgtcggg gccccgcccg ccagggccgc acgcgcgcgt gccgttcgta
      361 ccaggcgaga agttcggcgg ctgccggtgg aagcactttc ttccgcgccg gcgatcggac
      421 tgctacggtc atggcgccaa cgatgccata gggcccgtcc tcatgagcaa gccctcgcac
      481 agttttcccc gggcgctttc gcaactgctg aacgccaccc tgagcgacgt cctcaaggcg
      541 cagggctttg cgtcaaccga aatcatctcg cgctggagcg acattgtcgg gcccgaaatt
      601 gccggccaca gcgagccgct caggatcaac tggccgcgtg ccgccgtcga gaaggcggaa
      661 cccgccacgc tggtcttgcg ggttgaagga cccgcagcgc ttgaaatcca gcacctgtcg
      721 gcagtcatac tcgaacgggt caatcgcttc ttcggctggc aggccatcgg tcggatcgcc
      781 attcggcaag ccccgctcag gcgccgcgaa ccatcgccgc tccccaaaat cgataacggc
      841 gcggccgaac ggatcgccga tgcactcccg gacgtcaagg atgagaattt gcgtcaggcg
      901 cttggccggc tgggtgcggc gatcaaggga aattgagcca tccgaacgtc ttggcaggag
      961 ggcgtgccat tgccacaatt gtacagtcca gctagccaat agctgaattg accggcgttt
     1021 agctcaggag ccccctttgc cgatgacccg acgcgaattt tgccagacca cggccacctt
     1081 ggcgcttgcc accagcatgc tcagcttctc gtcgttgtcg ccgttcccgg gcgtggcttt
     1141 cgccgaaacg gttcccacgg acgaactgat gaagcccggt gcgctgcctg acatggcaat
     1201 gggcgacgcc aaggcgcccg taacaatcat cgaatatgcg tcaatgacct gtccgcattg
     1261 cgcgcatttc caggaggtga cgtttcccga actcaagaag cgctatatcg acacgggaaa
     1321 ggtccgctac attttccgcg aattcccgct cgacagtctc gctgcggcag ccttcatgct
     1381 tgcgcgctgc gccggcaagg atgacaagga caaatatttt gcgttgatcg acacgttgtt
     1441 ccgtcagcaa cggcaatggg cggttgaaaa gccgattccg cccctgctgg cgatcgcaaa
     1501 gcaggcgggc ttcaccgagc agactttcaa tgcctgtctt gcgaatcagc agatgctcga
     1561 cggcatcgaa aatatccgcc agcgggccat caagcagttc aaggtgcagt cgacgccgac
     1621 ttttttcatc aatggaacag ctcatcccgg cgctctctct atcgatgaaa tggccaagct
     1681 gatcgacccc tatctcaagg gttgataagg gttttttggc ctgaaaagca catcggcagg
     1741 gcgtggtttc gaccgcggct ttgcaatcgt ccgcctgaaa tgtctggaaa accgcgcccc
     1801 cgtcccgcag ggcagttgcc cttgcgagcg cgccgattca ttctccgtcc gacgcggcaa
     1861 acgggccgcc aaacccgggc caagccccgg ggaaggcttg ctgaatcagt gaatgcagaa
     1921 tctgaataga ccggcgggct ctctcgtccc gctttgcgca acgaccagga agtcgccgtt
     1981 cccgcaaatc cggcttggcg gtgcaggaag gtcgcagtgc gtgcgcccac aagaagaatg
     2041 caatgaggcc ctaacgcaat gaagctcaca aggctgcgcc tgctcggctt caagtccttt
     2101 gtagagccga ccgatttcat gatcgagccc ggattaaccg gcgtggtcgg tccgaacggt
     2161 tgcggcaagt cgaacctggt cgaagcactg cgctgggtga tgggcgaatc ctcgtacaag
     2221 tccatgcgcg cctcgtccat ggacgatgtc attttttccg gctcgaacac ccgccctgcc
     2281 cgcaataacg ccgaggtggc gatcgccatc gataattcgt cgcggtcggc ccctgccgcc
     2341 ttcaacgacc aggattcgct ggaaatcgcg cggcgcatcg agcgggattc cggctccaac
     2401 taccgtatca acggacgcga agtacgcgcc cgtgacgtcc agattctgtt cgccgacgcc
     2461 tctacaggcg cgcgttcgcc cgcactcgtg catcaggggc gcatcggcga gatcattcaa
     2521 gccaaacccg agcagcgccg gcgtgtgctg gaagaagcgg ccggcgtggc cggcctgcac
     2581 gcacggcgcc atgaggcaga gctgcggctc aaagcggcgg aaacaaacct tctgcgggtc
     2641 gaagatgtca taggccaact ctccgcgcag atcgacgggc tgaagaaaca ggcgcggcag
     2701 gccatccgct atcgcacggt gtcgtcgcaa gtgcgcaagg cagaggcgac gctctatcat
     2761 ttgcgctgga ccgccgccaa caacgaagtg gcggaagccg agcacgccaa agacgtgagc
     2821 gtacgcacgg ttgccgatcg tgtcgccgcc gaggcggacg catccaaacg tcaggccttg
     2881 gccgcggcgg cgttgcctga gctgcgcgag gccgaggcgc gcgccggtgc ggcactgcat
     2941 cgcctgaacg tagcgttgca ggatattgac cgggaagagg gccgcgccaa ggaccgcatc
     3001 gcagaactcg acctgcggct cgtgcaattt g
//
LOCUS       DASSRL010000198         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_145397, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000198 DASSRL010000000
VERSION     DASSRL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145397"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="tuf"
                     /locus_tag="VFL49_04125"
     CDS             <1..1078
                     /gene="tuf"
                     /locus_tag="VFL49_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7911191.1"
                     /translation="YVSYDEVAKASEAQGRRDASKILTIATSHVEYATEKRHYAHVDC
                     PGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVYMNKVDM
                     VDDPELLELVELEVRELLSKYNFPGDKIPIIKGSALKALDGSDEKLGKETIMELMKAV
                     DEYIPQPERPKDQPFLMPVEDVFSISGRGTVCTGRIERGIIKVGEEVEIVGIKPTTKT
                     VVTGVEMFRKLLDQGEAGDNVGCLLRGTKREEVERGQVLCKPGSVKPHTKFKAEADIL
                     TKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPQGTEMVMPGDNVAMEVHLIVPIAME
                     EKLRFAIREGGRTVGAGVVASIIE"
     gene            1228..1536
                     /gene="rpsJ"
                     /locus_tag="VFL49_04130"
     CDS             1228..1536
                     /gene="rpsJ"
                     /locus_tag="VFL49_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7911192.1"
                     /translation="MNGQNIRIRLKAFDHRILDASTREIVNTAKRTGAQVRGPIPLPT
                     RIEKFTVNRSPHVDKKSREQFEMRTHKRLLDIVDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            1556..>2042
                     /gene="rplC"
                     /locus_tag="VFL49_04135"
     CDS             1556..>2042
                     /gene="rplC"
                     /locus_tag="VFL49_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7911193.1"
                     /translation="MRTGVVAQKVGMTRVFTEAGEHVPVTVLRLAQCQVVAHRTKDKN
                     GYVALQLGSGPRRSNNMSKSDRGYFGKQKVEPKRKLAEFRVTEDSLIPVGAEITADHF
                     IPGQFVDVCGITIGKGFAGGMKRWNFGGLRASHGVSISHRSIGGTGGRQDPGKTFKNK
                     KM"
BASE COUNT          434 a          611 c          654 g          343 t
ORIGIN      
        1 gtacgtgtcg tacgacgagg tggccaaggc ctccgaggcc cagggccggc gcgacgccag
       61 caagatcttg accattgcga ccagccatgt cgaatacgcg acggaaaagc gtcattacgc
      121 gcatgtcgac tgtccgggtc atgccgacta cgtcaagaac atgatcaccg gcgccgcgca
      181 aatggatggc gctatcctgg tggtgtccgc cgccgacggc ccgatgccgc agacccgcga
      241 gcacattctg cttgcccgcc aggtcggcgt tccggcgctc gtcgtctaca tgaacaaggt
      301 cgacatggtc gacgatcccg agttgctcga gctggtcgag ctggaggttc gcgagcttct
      361 ctccaagtac aatttcccgg gcgacaagat ccccatcata aagggttcgg cgctcaaggc
      421 gcttgacggc agcgacgaga agctcggcaa agaaacgatc atggagctca tgaaggcggt
      481 ggatgaatac attccgcaac ccgagcgtcc aaaggaccag ccgttcctga tgccggtcga
      541 agacgtgttc tcgatctcgg gccgcggcac tgtgtgcacc ggtcgaatcg aacgcggcat
      601 catcaaggtc ggcgaggaag tcgagatcgt cggcatcaag ccgacgacca agaccgtcgt
      661 caccggcgtc gagatgttcc gcaagctgct cgaccagggc gaggccggcg ataacgtcgg
      721 ctgcctgctg cgcggcacca agcgcgagga agtcgagcgc ggccaggtgc tctgcaagcc
      781 cggctcggtg aagccgcaca cgaagttcaa ggcggaagcc gacatcctca ccaaggaaga
      841 gggtgggcgc cacacgccgt tcttcaccaa ttaccgtccg cagttctatt tccgcaccac
      901 cgacgtgact ggcgtggtgc acctgccgca aggcacggaa atggtgatgc cgggcgacaa
      961 tgtggcgatg gaggtgcacc tgattgtgcc gatcgccatg gaagagaaat tgcgcttcgc
     1021 cattcgcgag ggcggccgca ccgtcggcgc cggcgtggtg gcgagcatca tcgagtaaca
     1081 ggtcgatccg tcgtcatgcc cggccatagc cgttcgaaga acggcgtttt gtcaaacgcc
     1141 tatgtgccgg gcatccacga cttaggaaaa tgcagacgtg gatggccgga acgagcccgg
     1201 ccatgacggg acagagagac gagaaaaatg aacggccaga atatccggat ccggctcaag
     1261 gcgttcgatc accgcatcct tgatgcatcg acgcgcgaga tcgtgaacac ggccaagcgc
     1321 accggtgcgc aggttcgcgg cccgattccg ctgccgacgc gcatcgagaa gttcacggtg
     1381 aaccgttcgc cgcacgtgga caagaagagc cgtgaacagt tcgagatgcg cacgcacaag
     1441 cgcctcctcg acatcgtcga tccgacgccg cagacggtgg acgcgctgat gaagctcgat
     1501 ctggccgcgg gcgtcgatgt cgagatcaag ctctaaggcc gggatgggac tagcaatgcg
     1561 tacgggtgta gttgctcaga aggtcggaat gacccgggtg ttcaccgaag ccggcgagca
     1621 cgtgccggtc acggtgctgc gccttgcgca gtgtcaggtg gttgctcacc ggaccaagga
     1681 caagaacggc tacgtggcac tgcaactcgg ttcgggcccg cgccgctcca acaacatgag
     1741 caagtctgac cgcggctatt tcggcaagca gaaggtcgag ccgaagcgca agctcgccga
     1801 attccgcgtg accgaagact cgctcattcc agtcggagct gaaatcaccg ctgatcactt
     1861 catccccggt cagttcgtcg acgtctgcgg cattaccatt ggcaagggct ttgccggcgg
     1921 catgaagcgc tggaatttcg gcgggttgcg tgcctcgcac ggggtgtcga tttcgcaccg
     1981 ctcgattggc ggcactggcg gccgtcagga tccgggcaag acgttcaaga acaagaagat
     2041 gc
//
LOCUS       DASSRL010000199         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1002786, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000199 DASSRL010000000
VERSION     DASSRL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002786"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="VFL49_04140"
     CDS             complement(<1..1740)
                     /locus_tag="VFL49_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7911194.1"
                     /translation="MNRAAPEPKRNMGQPAPRLDGRLKVTGEARYASDFPVANPAYAF
                     LVTSTIAKGHIEKMELANALAVPGVIDILTYQNVDGLNEVKASASGGSATTSVQNLGP
                     QIWHDGQIVAIVLADSFEAAREGAYKMNVAYKSTPPSAGFGSAGVTEEDASKANERAK
                     NLPQAGDADAAIASADVVLEAEYGTPTQHHNPIELFTTTCAWNDGQLTIYEPSQFVFG
                     VKNSAAQKLGISAEKVHVVNPYVGGAFGSKGQFSPRTGFIALAAKKLNRPVKLVATRD
                     QGFTIQTYRAETRQRIRMGARRDGKIVGFVHEGREVTSRPDPYTVAGVEDSARLYHYG
                     AVKTHVTLVHADRNTPGFMRSPPVVPYIYALESAMDELAEKLDMDPIELRRVNDSMTD
                     SMGKQWSSRSLMRCYDEAAKVFGWSGRNPKPGTMRDGDWLVGWGCASAIYPTHVGAAA
                     ARVRLMADGQTRVQIAAHEIGTGAYTVIGQMAAERLGVPLSSVTVELGDTSLPPAPVA
                     GGSNTTASACCAVMKACDAIRRKLYGSANETVGGSAVPSEAAGDNSLEAAFRRRKLGA
                     IEEYAEYLPPGAKP"
     gene            complement(1743..>2451)
                     /locus_tag="VFL49_04145"
     CDS             complement(1743..>2451)
                     /locus_tag="VFL49_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET7911195.1"
                     /translation="ELAASAQIRNMASLGGNVLQRTRCTYFRDTSYGACNKREPGSGC
                     AAIEGINRSHAVLGTSASCIASYPGDFAQALIALDAEVELAGPRGSRRIHFAELHRPL
                     GQTPHVETILELGELILAFMVPPAPWAKRSLYLKVRDRQSYEFALASAAVALDIDGGT
                     VREARIALGGVSALPWRAHEAEQKLRGRKPDDDTFNEAASIAFAAAKPRQHNAFKVQL
                     GKLTLVRALRQAATMEL"
BASE COUNT          432 a          753 c          760 g          506 t
ORIGIN      
        1 gggtttggcg cccggcggca gatattcggc atattcttcg atcgcaccca gctttcgccg
       61 tctgaaggcg gcctcgaggc tgttgtcgcc agctgcctcg ctcgggacgg cgctgccgcc
      121 gacggtctca ttggcgctgc catagagctt gcgccggatg gcgtcgcacg ctttcatcac
      181 cgcgcagcag gcactcgccg tcgtgtttga gcctcccgcc acgggcgccg gcggcaggct
      241 cgtatcgcca agctcgaccg tcacagatga aagtggaacg ccgagacgct cggccgccat
      301 ctggccgatg acagtatagg cgccggtgcc gatctcatgt gccgcaatct gcacgcgcgt
      361 ctggccatcg gccatcagac gcacgcgtgc ggcggctgcg ccgacatgtg ttggatagat
      421 agcgctcgcg cagccccagc cgaccagcca gtcgccatcg cgcatggtgc ccggtttcgg
      481 gttacgcccc gaccaaccaa agacttttgc cgcctcgtca taacaccgca tcagcgagcg
      541 gctcgaccac tgcttgccca tgctgtcggt catggaatca ttgactcggc ggagctcgat
      601 tgggtccatg tcgagctttt cggccagctc atccatcgcg ctttcgagtg cgtagatata
      661 agggaccacc ggcggagagc gcatgaaacc cggtgtattg cgatcagcat ggacaagagt
      721 cacgtgagtc tttaccgcgc catagtgata aagccgtgcg ctgtcctcga cgccggcgac
      781 tgtatatggg tcggggcgtg aagtgacttc ccggccttcg tggacgaaac ctacaatctt
      841 gccgtcgcgt cgtgcgccca tgcggatacg ctgtcgcgtt tccgcgcggt aggtctggat
      901 ggtgaagccc tggtcacgcg tggcgaccag cttcaccggc cgattgagct tctttgccgc
      961 cagcgcgatg aagccggtgc gcggcgaaaa ttgtcccttt gagccgaacg cgccgcccac
     1021 ataggggttc accacgtgca ccttttcggc cgaaatacca agcttctggg cggcactgtt
     1081 tttcacgcca aagacaaatt ggctcggctc ataaattgtc agctggccat cgttccacgc
     1141 gcatgtggtg gtgaacagtt cgatcggatt gtggtgctgt gtcggcgtgc cgtattctgc
     1201 ctcaagcacg acgtctgcgg atgctatagc cgcgtccgca tcacctgcct gcggcagatt
     1261 cttggcgcgc tcattagcct tgctggcatc ttcctcggtc acgcccgccg agccaaaacc
     1321 ggcactcggc ggtgtcgact tgtacgccac attcatcttg taggcgcctt cgcgggcggc
     1381 ctcgaaactg tcggccagca cgatggcgac gatttgccca tcgtgccaga tctgcggccc
     1441 gaggttctgc acggaggttg ttgcgctgcc tccgctggct gaggctttca cttcgttcaa
     1501 gccgtcgaca ttttggtaag taaggatgtc gatcacgcca ggcaccgcaa gtgcgttcgc
     1561 gagctccatt ttttcgatgt gcccttttgc aatggtgctg gtgaccagaa acgcataggc
     1621 cggatttgcg acgggaaaat ccgaggcgta gcgcgcttcg ccggttacct tcaggcggcc
     1681 gtcgagacgt ggcgctggtt gtcccatgtt gcgcttgggt tccggtgccg cgcgattcat
     1741 aattacagct ccatggtcgc ggcctggcgc aacgctctca ccaaagtgag ctttccgagc
     1801 tggactttga aagcgttgtg ttggcgcggt ttggccgcgg cgaatgctat ggaagccgct
     1861 tcgttgaacg tatcgtcgtc aggcttgcga cctcgcaatt tttgctccgc ctcgtgggcg
     1921 cgccagggca atgccgaaac gccgccaagc gcaatgcggg cttcacgaac agtgccgccg
     1981 tcaatatcga gtgcgacagc ggcggaagcg agagcgaatt catacgactg gcgatcgcga
     2041 accttcaggt acagcgaacg cttcgcccac ggggccggtg gcaccatgaa ggcgagaatg
     2101 agttcgccca gctccaggat cgtttcgaca tgtggcgttt ggcccaaggg acgatgcaat
     2161 tccgcgaaat ggatccggcg cgagcctcgc gggccggcca actcaacctc ggcgtcaagc
     2221 gcgatcagcg cttgggcgaa atcgccgggg taagaagcaa tgcagctcgc gctcgtgccg
     2281 agcactgcat gggaacggtt aatgccctcg atcgcagcgc agccggagcc aggctcgcgc
     2341 ttgttgcagg cgccataaga cgtgtccctg aaataggtgc agcgcgtgcg ctgtaaaaca
     2401 ttgccgccga gcgaagccat gttgcggatt tgcgcgctcg cggcgagctc c
//
LOCUS       DASSRL010000200         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_22781, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000200 DASSRL010000000
VERSION     DASSRL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22781"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VFL49_04150"
     CDS             complement(<1..1311)
                     /locus_tag="VFL49_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7911196.1"
                     /translation="MAEQRTQRRLAAIMAADVVGYSRLMEADEAGTMAALKARRKDVL
                     EPLVARHQGRIFKVTGDGVLVEFASAVNAVQCAVDLQQGMTAANDGQPERIVLRIGVN
                     LGDVMFEGGDLYGDGVIIAVRLQTIAEAGGILVSHAVQEHVTRKLAVRFTDQGERALK
                     NIARPIRVYSVSWECAPDGITPPSARGRPDLPDKVSVAVLPFVNMSTDAEQTFFADGL
                     AEDLITDLSKVPGLLVIARNSSFSYKGRSVDTRTVARELSVRYVVEGSVRRAATRVRI
                     NAQLIDAVSGSHLWADRFDRDLTDIFVVQDEVVAKIVGALVGALPATLMPPKHRTTNL
                     GAYELFVRGRSMVSLSLQETRTARLLLGKALDLDPDFVEAHAWVAMSHIIGWAYCDEG
                     DEHRMLARSAARKSMLLDAENADAHIILGYLRAFEGELSEGVSEV"
     gene            complement(1426..2427)
                     /locus_tag="VFL49_04155"
     CDS             complement(1426..2427)
                     /locus_tag="VFL49_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7911197.1"
                     /translation="MIVESPSHAMLISIMIFLFVPDKGNFYSILMERPGPVRNVTRQR
                     KGARAAADRSTARRPANTKPARSYADLNREISGRYESLSKRLRQIAEFALKHPNEIAI
                     ESIAVIAQHAGVQPSALIRFAKAFGFEGFSDLQRIFQQRLRESRPSYSERIAAMREEL
                     NAQRDHGPQAILTRFAEANIAALQHLCEETKAGDLDRAVQILRRAETIHLVAQRRSFP
                     LAAYLYYALSKIGRRAHLIDAVGGMDTEQRQLMEPRDALVAVTYADYTPRVVETVTFA
                     AQRKVPVIGITDQPLSPLTAQCDVVFYVEDAAVHDFRSLGASMCLAQALVVALGATG"
     gene            2606..3853
                     /locus_tag="VFL49_04160"
     CDS             2606..3853
                     /locus_tag="VFL49_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911198.1"
                     /translation="MAGCASAIALGSLLPALALAADIVLAVPNGSEGDGLRAAAADYQ
                     KLKGITIDIVQAPYANLFDKAANAGASKSGAYDIVLMDDPWLPFFAENGYLEPLASYF
                     KAIGTDGPDSDFLAKSVALCRNPYNTGPYVCLPYVGNAQMFFYDPDEFKKQGLPDGPK
                     TWDDVIKAAAAVTKAGNGERFGYVMRAAEGNPVVADFMPIFWSYGGDLFDKDRKPNLL
                     TPEAKKAVEVFLKLKEVSPPGVESFQADEVGTYIAQGTAASAINWPNWVATFEDPAQS
                     KVVGKIAYSAIPSGTKAGSSEIGHWLMGIMAAAPHKQAAFDFMIWATSPEQIKKSAER
                     GNPPVRFSVFTDPELTSQKQFRHFPILMQAINFSTPRPRHPKWPEIENAFGAELSKAV
                     AGAETGDEALANAQKAIEEILAR"
     gene            3985..>4703
                     /locus_tag="VFL49_04165"
     CDS             3985..>4703
                     /locus_tag="VFL49_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7911199.1"
                     /translation="MSEWIDRRLKFVFLAPAIIVLIALTAFPTVYMFVAAFVKFDASA
                     PLNSKFAGIDNFLRLFTDDKFHNALINTAVFSIVAVALEFVLGLAFALLVDTYIRRAN
                     FIKTLLILPMMMPTIVVAITWKLMYQPQFGVINDLLGRIGVPPLLWAASVKTAMPSII
                     LIDIWEWTPFIFLMCLAGLASLPQEPFEAAELDGASAFQKFRDLTLPFLKPVIVVTLL
                     LRIMDAFRLFDQVYVLTLGGPA"
BASE COUNT          891 a         1472 c         1443 g          897 t
ORIGIN      
        1 gacttcggag acaccctcgg acaattcacc ctcaaaggct cggagatagc ccagaatgat
       61 gtgcgcatcc gcgttctcag catcgagcaa catggacttc cgtgcggccg aacgcgcgag
      121 catccgatgc tcgtcccctt cgtcacagta ggcccagcca atgatgtggc tcatcgccac
      181 ccaagcgtgc gcttcgacaa agtcgggatc gagatcgagt gccttcccta gcagcagtcg
      241 cgcagttcta gtctcctgca gcgatagaga caccatggac cgcccgcgaa cgaacaattc
      301 ataggctccg agattcgtcg tcctgtgttt tggaggcatg agcgtcgcgg ggagcgcgcc
      361 gacaagcgcg cccacgatct tagccacgac ctcatcctga accacaaaga tgtcggtaag
      421 atctcggtca aagcgatcag cccagagatg gctgccgctc actgcatcga tgagttgagc
      481 attgatgcgc acccgcgttg cggctcgcct tacgcttcct tcgactacat agcgaacgct
      541 gagttcccta gcgaccgtgc gggtatcgac cgaacgtcct ttgtaggaaa agctcgaatt
      601 gcgcgcaatg acgagaaggc cgggaacctt ggatagatcg gtgatcagat cttccgccag
      661 gccgtctgcg aaaaacgtct gctcggcatc ggtactcata ttgacaaagg gcagcacggc
      721 gaccgagacc ttgtccggca gatctggccg cccccgcgcc gatggcggcg taatgccgtc
      781 cggagcacat tcccaactga cactgtagac acggattggt cgtgcgatgt tcttcagcgc
      841 gcgttcgccc tgatcggtga agcgcacagc aagcttgcga gtgacatgct cctgcacggc
      901 atgggagaca agtatgccgc ctgcctcggc gatggtctgc agccgaacag cgatgatgac
      961 gccatcgcca tagaggtcgc cgccctcgaa catgacgtcg ccgagattga cgccgatgcg
     1021 cagcacgatg cgctctggct gaccgtcatt cgcagcagtc atgccctgct ggagatcgac
     1081 cgcgcattgc acagcgttga cggcactggc gaactccacc agtacgccgt caccggtcac
     1141 tttgaaaatc cggccctgat gccttgcgac tagcggctcc agcacgtcct tacggcgcgc
     1201 cttgagcgcg gccatggtcc cggcttcgtc agcctccatg agcctgctgt aaccgaccac
     1261 gtcagcggcc atgatggctg cgagacgacg ttgagtgcgc tgctcggcca tgcctgccga
     1321 taccctaggt aagacgcttc aaaattgtat cgtcgggttc tggcaatgtc tatcagactg
     1381 gatttaagat cgccttcgat tctctagata gccacagctg gcttcctacc ccgtcgcccc
     1441 cagcgccacc accagcgcct gcgccaggca catcgacgcg ccgagggagc ggaagtcgtg
     1501 caccgcggcg tcctcgacat agaacaccac gtcgcactgc gcggtgaggg ggctgagcgg
     1561 ctggtcggtg atgccgatga ccggcacctt gcgctgcgcg gcgaaggtga cggtctccac
     1621 cacgcgcggc gtgtagtcgg cataggtgac ggcaaccagc gcatcgcgcg gctccatcaa
     1681 ttgccgctgt tcggtatcca tgccgccgac cgcatcgatc aggtgcgcgc gccgtccgat
     1741 cttgctgagg gcgtagtaga ggtacgccgc gagcgggaag gagcggcgct gcgccaccag
     1801 atggatggtc tcggcccgcc gtaggatctg cacagcgcgg tcgaggtcgc ccgccttggt
     1861 ttcctcgcac agatgctgga gggcggcaat gttggcctcc gcgaagcgcg tcaggatcgc
     1921 ctgcggccca tgatcgcgct gggcattcaa ttcctcgcgc atcgcggcga tgcgctcgga
     1981 atagctcggg cggctttcgc gcaggcgctg ctggaagatg cgctgcaggt cggagaagcc
     2041 ctcgaacccg aacgccttgg cgaagcggat gagggcggag ggctgcacgc cggcatgctg
     2101 ggcgatgacg gcgatggatt cgatcgcgat ctcgttggga tgcttcaggg cgaactcggc
     2161 gatttgccga aggcgcttgc tcaggctctc atagcggcct gagatctctc ggttcaggtc
     2221 ggcgtagctc cgcgctggct tggtgttggc cggacgcctg gcggtcgacc gatcagccgc
     2281 ggctcgcgct ccctttctct gtcttgtcac gtttctgacg ggtcctggcc tctccatcaa
     2341 aatagaataa aaattcccct tgtcgggcac aaacaaaaaa atcataatgc taatcaacat
     2401 cgcatgggac gggctctcca cgatcatcgg tgggagctgg tttgtgctga atcgcttcgc
     2461 cagacgcagc tgtcggcgag gctcaaaaaa ggcactttga gaagcgtcag atcaccgcgg
     2521 atcaaccgcg ggcccgcttg gatgccaaca cactgggaga aatccgatga tcaaagaatg
     2581 gactcaacgc cacaagctcg tccgcatggc cggctgcgcg tcggccattg cgttgggatc
     2641 gctgttgccg gcgctcgcat tggccgccga catcgtgctg gccgtgccga acggttccga
     2701 gggcgacggc ctgcgcgcag ccgcggccga ttatcagaag ctgaagggca tcaccatcga
     2761 catcgtgcag gcgccgtacg ccaatctgtt cgacaaggcg gcgaacgccg gcgccagcaa
     2821 gtcgggcgcc tacgacatcg tgctgatgga tgatccgtgg ctgccgttct tcgccgagaa
     2881 cggctatttg gaaccgctcg cgagctattt caaagcaatc gggacggatg gcccggacag
     2941 cgactttttg gccaaatcgg tcgcgctttg ccgcaatccg tacaacaccg gcccctatgt
     3001 atgcctgccg tatgtcggca atgcccagat gttcttctac gaccctgacg aattcaagaa
     3061 gcagggcctg cccgacggac ccaaaacctg ggacgacgtg atcaaagccg cggcggcggt
     3121 gaccaaggcc ggcaacggcg agcgcttcgg ctatgtcatg cgcgcggccg agggcaatcc
     3181 ggtggttgcc gacttcatgc cgatcttctg gtcctatggc ggcgatctgt tcgacaagga
     3241 ccgcaagccg aacctcctga cgccggaagc caagaaggcg gttgaagtat tcctgaagct
     3301 aaaggaagtc tcgcccccgg gcgtcgagag cttccaggcg gacgaggtcg gtacctacat
     3361 cgcacagggc acagccgcct cggcgatcaa ctggccgaac tgggtggcga cattcgagga
     3421 tcccgcgcag tccaaggttg tgggcaagat cgcatacagc gcgatcccgt ccggcaccaa
     3481 ggcgggtagc tccgagatcg gccactggct gatggggatc atggctgccg cgccgcacaa
     3541 gcaggcggcg tttgatttca tgatctgggc gacctcgccg gagcagatca agaagtcggc
     3601 cgagcgcggc aacccgccgg tgcgcttctc ggtcttcact gatcctgagc tgacgtcgca
     3661 gaagcagttc cgccacttcc cgatcctcat gcaggccatc aacttctcga ccccgcggcc
     3721 gcgtcaccca aagtggccgg agatcgagaa cgccttcggc gccgagttgt cgaaggcggt
     3781 ggccggcgcc gagaccggcg acgaggcgct tgccaacgcc cagaaggcga tcgaggagat
     3841 cctggcgcgg tagagccgag gggggcggaa tgtcgatgcg acgcaacatt ccgccctcgc
     3901 ttttttttca tcgatcaggc gcatgatatg tcaccacctg cgggcacgcg gccggcgaat
     3961 gcccgttgat cgggaaaagg cgacatgagt gagtggatcg accggcgcct gaagttcgtc
     4021 tttctggcac cggccatcat cgtgctgatt gcgctgacgg cgttcccgac cgtctatatg
     4081 ttcgtggcgg ctttcgtgaa attcgatgca tcggctccgc tcaacagcaa gttcgccggc
     4141 atcgataatt ttctgcggct tttcaccgac gacaagttcc acaacgcgct catcaatacc
     4201 gcggtgttct cgatcgtcgc ggtggcgctg gagttcgtgc ttgggctcgc cttcgcgctg
     4261 ctggtcgaca cctacatccg gcgcgccaac ttcatcaaga cgctgctcat cctgcccatg
     4321 atgatgccga cgatcgtggt cgcgatcacc tggaagctga tgtaccagcc gcagttcggc
     4381 gtcatcaacg acctgctggg ccggatcgga gttccaccct tgttgtgggc cgcgagcgtc
     4441 aagaccgcca tgccgtccat catcctcatc gatatctggg aatggacgcc cttcatcttc
     4501 ctgatgtgcc tggcggggct tgccagcctg ccgcaggagc cgttcgaggc ggcggagctg
     4561 gacggcgcct ccgccttcca gaagttccgc gatcttacct tgccgttcct gaagccggtg
     4621 atcgtcgtga cgctgctgct ccggatcatg gatgcgttcc gcctgttcga ccaggtctac
     4681 gtcctgacgc tgggaggacc cgc
//
LOCUS       DASSRL010000201         5071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1667589, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000201 DASSRL010000000
VERSION     DASSRL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667589"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFL49_04170"
     CDS             complement(<1..1227)
                     /locus_tag="VFL49_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET7911200.1"
                     /translation="MAKSPPKKPQKHKDPAKPTRAKAARPEQSPTPDALADLLNPAIK
                     KGTAGLGSGTGLQPPPDNSWDRRRDFSEAHRARKSTPKEFNEAQQADFEGPPVTGLDP
                     KLADELGLTDEAKYRLPRADLPTQPGGFGGIASQQSLERLLREGRPELSAVPWMPHRP
                     PRPEKSEGGRKLVIASDFEAKGDQPQAIKELVEGVQRNDRTQVLLGVTGSGKTFTMAK
                     VIEATQRPALVLAPNKTLAAQLYGEFKSFFPDNAVEYFVSYYDYYQPEAYVPRTDTYI
                     EKESSINEQIDRMRHSATRALLERDDVIIVASVSCIYGIGSVETYSAMTFSLKQGGRI
                     DQRQLLADLVALQYKRTQGDFYRGSFRVRGDTVDIFPAHYEDRAWRIHLFGDEIEKIE
                     EMDPLTGQKTDELEFVK"
     gene            1367..2368
                     /locus_tag="VFL49_04175"
     CDS             1367..2368
                     /locus_tag="VFL49_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HET7911201.1"
                     /translation="MGETMLKWSRQLALCVATLFAILIVIGPVRAQTKLKWAHVYEVA
                     EPYHANALWAAEEIKKRTDGRYEIAVFPASQLGNENQINEGLGLGTVDIIYTGASFAG
                     SIHKPLAITGAPFILRDFDHWKAYRDSKLFRDLAKGYEDKTRHKVVAILYYGQRMLIA
                     NKEIRKPEDMKGMKLRVPPAPLFLMFTKSVGANATPIAFAEVYLALQQGTVDGQENPL
                     PTIMAKKFYEVQSHIMLTGHITESLVTIVGTHVWSKFSDADKKVFEDVLKEASARSTD
                     QIRASELKLADEFRKLGKTVVEVDREAFRKAALPLHNDASAGAGWSKAEYDALQALK"
     gene            2450..3004
                     /locus_tag="VFL49_04180"
     CDS             2450..3004
                     /locus_tag="VFL49_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET7911202.1"
                     /translation="MSEKKSPGDQRLIEAKDSEVVIEHYPEDWIAFVIFWSLAFIVFL
                     QFFTRYVLNDSLSWTEEIARYGLMWIVFIGGAMVTRRNTHIAVELLSNVMRPGPLRAA
                     LLALVDFIKLAFLGLLAFVSWTITERMHYQRMTVFDLPMSYVYGGVALGCFLMLIRQA
                     INVWRNARIGWSRPDDVTHQISAD"
     gene            3159..4439
                     /locus_tag="VFL49_04185"
     CDS             3159..4439
                     /locus_tag="VFL49_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7911203.1"
                     /translation="MIVMAFLFIVVLLVGVPVFIALAGSSFIYTHFIAGLPDFVILHR
                     MAGGIDSFPLLAVPFFILAGNLMNSAGITNRIYDFAVALAGWTRGGLAHVNIIGSVIF
                     AGMSGTAIADAAGLGTIEIKAMQDHGYDAKFSVGVTAASSTLGPIIPPSLPFVIYGMM
                     GNVSIGALFLGGVIPGLVMTACMMGYVYYCARRYRMGRDQAFQWRALALTFIAAFPAL
                     LTPAIIVGGMTFGWFTPTEAAIAACAWAMLLGVFLYRTLTLRQFYKVSMDTIETTAGV
                     LLIVGAASLFGWVLTTTQVTEYTTQSLLALTDNRYVILLLINILLLVIGCFLEPIASI
                     SILVPVLMPVIVKVGIDPVHFGVMMTLNLMIGLLHPPLGMVLFVLSRVAKLSIEQTTM
                     AILPWLIPLLLSLVAITMIPELTLWLPRVVGMIK"
     gene            complement(4476..>5071)
                     /locus_tag="VFL49_04190"
     CDS             complement(4476..>5071)
                     /locus_tag="VFL49_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7911204.1"
                     /translation="WADDIEAVIDELALDRPILVGWSYGGYIISDYIRRRGQKRISGI
                     NFVAAAVVLGPKAFGPLIGPGFLENAPGGCQKDLAENIAAIRRFLRACIVKPVSQDEF
                     EEILAFNMAVKPGVRGALIQRELDFAPVLEAITLPVLVTHGRADTVVLPAMADYILQH
                     CRTAKASWFDGVGHTPFLEEPSRFNAELKDFARNAHR"
BASE COUNT          898 a         1509 c         1561 g         1103 t
ORIGIN      
        1 cttcacgaat tccagctcgt cggtcttctg gccggtgagc gggtccatct cctcgatctt
       61 ctcgatctcg tcgccgaaca gatgaatgcg ccaggcgcgg tcttcgtagt gcgccggaaa
      121 aatatcgacg gtgtcgccgc gaacgcggaa ggagccgcgg tagaaatcgc cctgcgtgcg
      181 cttgtattgc agtgcgacca ggtcggcgag cagctggcgc tggtcgatcc tgccgccctg
      241 cttcagcgag aacgtcatcg ccgaataggt ttccaccgag ccgatgccgt agatgcacga
      301 caccgacgcg acgatgatca cgtcgtcacg ctcgagcagc gcccgcgtcg ccgagtgacg
      361 catgcggtcg atctgctcgt tgatcgagga ttccttctcg atataggtgt cggtgcgcgg
      421 gacgtaggct tccggctggt agtagtcgta ataggagacg aaatactcca ccgcgttgtc
      481 cggaaagaac gacttgaatt cgccatagag ctgcgcggcg agcgtcttgt tgggcgcgag
      541 gacgagggca gggcgctggg tcgcctcgat caccttcgcc atagtgaagg tcttgcccga
      601 accggtgacg ccgagcagaa cctgcgtgcg atcgttgcgc tggacgccct cgaccagttc
      661 cttgatcgcc tgcggctggt cgcctttcgc ctcgaagtcc gacgcaatca ccagcttcct
      721 gccgccttcc gacttttccg gccgcggcgg gcgatgcggc atccacggga cggcggagag
      781 ctccggcctg ccttcgcgca gcaacctttc cagagattgc tggctcgcga ttccgccaaa
      841 accgccgggc tgtgtcggca agtcggcgcg cggcaggcgg tattttgcct cgtcggtcag
      901 gccgagttca tcggctaatt tcgggtcaag gccggtgacc ggcgggccct cgaagtccgc
      961 ttgctgcgcc tcgttgaatt ccttcggcgt cgatttgcgc gccctgtgcg cctcggaaaa
     1021 atcgcgccgg cgatcccacg aattgtcggg cggcggctgc aatcccgtgc ctgaaccaag
     1081 gccggccgtg ccctttttga tcgcggggtt gagcaagtcc gcgagggcgt cgggcgtagg
     1141 cgattgctcc ggccgcgccg ccttggcgcg tgtcggtttc gccggatcct tgtgcttctg
     1201 gggctttttc ggaggagatt tcgccattgc cccgaatatg ggacgagtcc tgtggcgagg
     1261 aaagggcagc cggggaatga gctttccgcg tgtcgcggcg ggcgccgttg cgcgaggcgg
     1321 accgcatagt aacatctttg cctgccgggt ttcgtacagg cggtaaatgg gggagacaat
     1381 gttgaaatgg tcgcgccaac tggcgctgtg tgtcgcgacg ctgttcgcga tattgatcgt
     1441 gatcgggccg gtgcgggccc agaccaagct caaatgggcg cacgtctatg aagttgcgga
     1501 gccctaccac gccaacgcgc tgtgggcggc ggaggagatc aagaaacgca ccgatggccg
     1561 gtacgagatc gctgtttttc ccgcctccca gctcggcaat gagaaccaga tcaacgaagg
     1621 cctcggactt ggcaccgtcg acatcattta tacgggcgca tcgttcgctg gttcgattca
     1681 caagccgctc gcgatcaccg gcgcgccgtt catcctgcgt gacttcgatc actggaaggc
     1741 ctatcgcgac agcaagctgt ttcgcgatct cgccaagggc tacgaagaca agacgaggca
     1801 caaggtcgtc gcgatcctct attacggcca gcgcatgctc atagccaaca aggagatcag
     1861 gaagcccgag gacatgaagg gcatgaagct gcgcgttccg ccggcgcccc tgttcctgat
     1921 gttcacaaaa tcggttggcg ccaacgcgac gccgatcgcc ttcgccgaag tctatcttgc
     1981 ccttcagcag ggcacggtcg acggccagga aaatccgctg ccgacgatca tggcgaagaa
     2041 gttctacgaa gttcagtcgc acatcatgct gaccgggcat atcactgaat ccctggtcac
     2101 tattgttggc actcatgtgt ggtcgaagtt ctccgacgcc gacaagaagg tcttcgagga
     2161 tgtgctgaag gaagcaagcg cgagatcgac cgaccagatt cgcgcgtccg agctcaagct
     2221 cgccgatgag ttccgcaagc tcggcaagac cgtggtggag gtcgaccgtg aggccttccg
     2281 caaggcggcg ctgccgctgc acaacgacgc cagcgccggt gccggctggt cgaaggccga
     2341 atacgacgcg ctgcaggcct tgaaataggt cacaaacgag cgattgcatc atgccgcgcg
     2401 cgacgcggca tgatgcgtgc acttaatgcg tgcagagcgc ggggcatcca tgagtgaaaa
     2461 gaaatcgccg ggcgatcagc gcctgatcga ggccaaggat tccgaagtcg tcatcgaaca
     2521 ttatccggag gactggatcg cgttcgtcat cttctggtct cttgccttca tcgtattcct
     2581 gcagttcttc acgcgttatg tgctcaatga cagcctgtcc tggaccgagg agatcgcgcg
     2641 ctacgggctg atgtggatcg tgttcatcgg cggcgccatg gtgacgcggc gcaacacaca
     2701 cattgccgtc gaactgcttt cgaatgtaat gagacccggg cccttgcgcg ctgcgctgct
     2761 ggcccttgtc gattttatca agctcgcttt tctcggtctg ctcgccttcg tctcatggac
     2821 gatcaccgag cgtatgcact atcagcgaat gacggtgttc gatctgccga tgagctatgt
     2881 ctacggcggc gtcgccctcg gatgcttcct gatgctcatc cgccaggcga tcaatgtgtg
     2941 gcgaaacgcc cgcatcggct ggagccggcc ggatgacgtg acgcatcaga tctccgcaga
     3001 ctaatgtttg atccagttga attgaatcgc gtctttatcc gctcctcccc gcgaaagcgg
     3061 ggacccaggc ccaaactgcc ggattcccgc tttcgcggga atgagcggaa tttgcgtctt
     3121 cagtatcgac agatttcaag ctcgctcagg tcagtcccat gatcgtcatg gcatttctgt
     3181 tcatcgtggt gctgctcgtc ggcgttccgg tcttcattgc gctggccggc tcgtccttta
     3241 tctacacgca tttcatcgcc ggtttgccgg acttcgtcat tttgcaccgg atggcgggag
     3301 gcatcgactc gtttccgctg ctcgcggtgc cgtttttcat tctcgccggt aatctgatga
     3361 attccgccgg catcaccaac cgcatctacg attttgccgt tgctctcgcg ggctggacgc
     3421 gcggcgggct cgcgcacgtg aacatcatcg gctccgtgat cttcgccgga atgtcgggca
     3481 ctgcaattgc cgacgcggca ggccttggca cgatcgagat caaggcgatg caggatcacg
     3541 gctacgacgc gaaattttcg gtcggtgtca cagctgcatc gtcgactctc gggccgatta
     3601 tcccgccgtc tttgcctttc gtgatttacg gaatgatggg caacgtctcg atcggcgcat
     3661 tgtttctcgg cggcgtcatt cccggccttg tgatgaccgc ctgcatgatg ggctacgtct
     3721 attactgtgc ccgccgctat agaatgggcc gcgaccaggc ttttcaatgg cgcgcgctgg
     3781 ccttgacttt cattgcggcg tttcctgccc tgctgacgcc ggcgattatt gtcggcggca
     3841 tgaccttcgg atggttcacg cccaccgagg cggcgattgc ggcctgtgcc tgggccatgc
     3901 tgctcggggt cttcctttat cgcacgctca cgctgcggca gttctacaag gtgtcgatgg
     3961 ataccatcga gaccacggcc ggtgtgctgc tcattgtcgg cgccgcctcg ctgttcggct
     4021 gggtgctcac aacgacccag gtgaccgaat acaccaccca gtcgctgctg gcgctcaccg
     4081 acaatcgcta cgttatcttg ctgctgatca atattctgct gctggtcatc ggttgtttcc
     4141 tggagccgat cgcgtcgatc agcatcctcg tgccggtgct gatgccggtg atcgtaaagg
     4201 tcggcatcga cccggtgcac ttcggggtga tgatgacgct gaacctgatg atcgggttgt
     4261 tgcacccgcc gctcggcatg gtgctgttcg tgctgtcgcg agtcgccaaa ttgtcgatcg
     4321 agcagacgac gatggccatc ctgccctggc tgataccgct gttattgtcg ctggtggcga
     4381 ttacgatgat cccggaactg accctgtggc tgcccagggt cgtggggatg atcaaatgaa
     4441 gagccttgcg gctgcgaacg gcttttcccg tcgcgttagc ggtgcgcgtt gcgggcgaaa
     4501 tccttcagtt ccgcattaaa gcgtgatggt tcttccagaa acggcgtgtg gccgacgccg
     4561 tcgaaccagg aggcttttgc ggtcctgcaa tgctgaagga tgtaatcggc catggcgggg
     4621 agcacgacgg tatccgcgcg cccgtgcgtg acgaggacgg gcagcgtgat tgcttcaagg
     4681 acgggggcga aatccagctc gcgttggatc aaggcgccgc gcacgccggg ttttaccgcc
     4741 atgttgaacg cgaggatttc ctcgaattcg tcctgcgaaa ccggcttgac gatgcaggca
     4801 cgcaggaacc tgcgaattgc cgcgatgttt tccgcaaggt ccttttgaca gccacccggt
     4861 gcgttctcga gaaatccggg ccctatcagc gggccgaagg ccttcgggcc gaggacgacg
     4921 gcagcggcga caaaattgat acccgaaatc ctcttctgtc ctcttctgcg aatgtaatca
     4981 gaaatgatgt agccgccgta agaccagccg accaggatcg gccggtcgag cgccagttca
     5041 tcgatcacgg cctctatgtc atcggcccat t
//
LOCUS       DASSRL010000202         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_967692, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000202 DASSRL010000000
VERSION     DASSRL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967692"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFL49_04195"
     CDS             <1..225
                     /locus_tag="VFL49_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7911205.1"
                     /translation="ATIKGVVDRLHLRGLVTALSDPNDRRRRAVALTDRGRRATEAAM
                     QVAADITAATLTPLTADEQRMAVKLLRKLS"
     gene            240..1124
                     /locus_tag="VFL49_04200"
     CDS             240..1124
                     /locus_tag="VFL49_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7911206.1"
                     /translation="MTLFKVGQATITRIEETNLPGYQLRDIFPDFTDVLLAEHKSWLA
                     PHHYEAETGKIILSVHSWLLQVGGKKILIDSCCGNNKVKPGRPFWHMLNVPYLERLEA
                     AGVRPEEIDLVMCTHLHHDHVGWNTLQRDGKWVPTFPNARYVFSKPDVDYFSKVDADP
                     KEGPAELGTFRECVIPILEHGKADLVSGGPHRLNEFIEIDSAPGHSPGHVFFKLESKG
                     ERAAFIGDVWHHLLQVYYPDWNFPKNSDAAQARVSRRKVLDYCASSGALVFPGHVGLP
                     FAGRIEGGKKGFVPHFAP"
     gene            complement(1129..1971)
                     /locus_tag="VFL49_04205"
     CDS             complement(1129..1971)
                     /locus_tag="VFL49_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7911207.1"
                     /translation="MPPIDSTVIPKLKAPAGTCDTHMHIYDGKYPKAPTARFVPPDAA
                     VSAYRKVCTRLGIERTVVVQPSTYGKDNRCTLAAVATMGPNARCIVVVDESVTDTELE
                     RLTRLGARGIRFFMLPGAPLPWEILETMSARVAPFGWHVQLQLDGRDLPGREVMLKRL
                     TSTLVIDHVGKFLEPVPLDHPGFKVLQRLVDGGRTWIKLSAPYEVSKSGPPTYDDVGK
                     LAKVLAKDAPDRMLWATNWPHPTPGAPVPDDAWMLDMLLDWIPDEAARHKALVENPAQ
                     IYGF"
     gene            complement(1981..>2199)
                     /locus_tag="VFL49_04210"
     CDS             complement(1981..>2199)
                     /locus_tag="VFL49_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7911208.1"
                     /translation="PPYGLCLLMASKFVGIKFSKAMLASLPIYIVFLAAIAFTIYFPD
                     IVLWLPKHLLPQSVGCFPNPNGTGYICP"
BASE COUNT          440 a          664 c          693 g          402 t
ORIGIN      
        1 gcgaccatca agggcgtcgt cgatcgcctc cacctgcgcg gtttggtgac cgcgctcagc
       61 gatcccaacg atcgccgtcg gcgagcggtc gcgctcactg atcgtgggcg gcgggccacc
      121 gaagcggcaa tgcaggttgc agcggacata actgcagcga cgctgacgcc gctcactgcg
      181 gacgagcagc ggatggcggt caagctgcta cgcaaactgt cctgacatcg tccgcggaga
      241 tgacattgtt caaagtcggc caggcgacga ttacccggat cgaggaaacc aatctgccgg
      301 gctatcagct cagggacatt tttccggact tcaccgacgt gcttttggca gagcacaaaa
      361 gctggctggc gcctcatcac tatgaggctg aaaccggcaa gatcatcctt tcggtgcaca
      421 gctggttgct gcaggtcggg ggcaagaaga tcctcatcga ctcctgctgc ggcaacaaca
      481 aggtgaagcc cggccgtccg ttctggcaca tgctgaacgt tccctacctc gaacggcttg
      541 aggcggccgg agtgcggccg gaagagatcg atctcgtaat gtgcacccac ctgcaccacg
      601 accacgtcgg ctggaacacg ctgcaacgcg acggcaaatg ggtaccgacc tttcccaacg
      661 cacgctatgt cttctcgaag cccgacgtcg actatttttc gaaggtcgac gccgacccga
      721 aggagggacc ggcggagttg ggaacgttcc gcgagtgcgt tattccgatt ctcgagcacg
      781 gaaaagccga cctggtgagc ggcgggcctc accggctcaa cgagttcatc gagatcgatt
      841 cggcgcccgg ccattcgccg ggacacgtgt tcttcaagct ggagagcaaa ggcgagcgcg
      901 ctgcgttcat cggtgatgtc tggcaccacc tgctgcaggt ctattacccg gactggaatt
      961 tcccgaagaa ctccgatgcg gcgcaggccc gcgtcagccg gcgcaaagtg ctcgactatt
     1021 gtgcatcaag cggggcactg gtgttcccgg gtcatgtggg gctaccgttt gccggccgca
     1081 tagaaggcgg gaaaaaaggc ttcgttccgc acttcgcgcc ctgacgaatc agaaaccgta
     1141 gatctgcgcc ggattttcca cgagggcctt atgtcgcgcc gcctcgtccg gaatccagtc
     1201 gagcagcatg tcgagcatcc aggcatcgtc aggcaccggc gcgcccggcg tcgggtgcgg
     1261 ccagttggtc gcccaaagca tgcggtctgg cgcgtctttc gccagcacct ttgcgagctt
     1321 gccgacatcg tcgtaggtcg gcggcccgga tttcgacact tcatatggcg ccgacagctt
     1381 gatccatgtg cgaccgccgt cgaccagcct ctgcagcacc ttgaatcccg gatgatcgag
     1441 cggcaccggc tcgaggaact tgccgacgtg gtcaatgacc agcgtgctgg tcaatcgctt
     1501 gagcatgacc tcgcggccgg gcaggtcgcg gccgtcaagc tgcaattgca cgtgccagcc
     1561 gaacggcgca acgcgcgccg acattgtttc gaggatttcc cacggcagcg gcgcgcccgg
     1621 cagcatgaag aaacggatgc cgcgcgcgcc gagcctggtc agacgctcga gttccgtatc
     1681 ggttacgctt tcatcgacga cgacaatgca gcgcgcattc ggtcccatcg tagcgactgc
     1741 agccagcgtg cagcggttgt ctttgccgta agtggacggc tgcacgacga ccgttcgctc
     1801 gataccgagg cgagtacaga ccttgcgata ggcggaaacg gccgcatccg gtggcacgaa
     1861 cctggcggtc ggcgccttgg gatatttgcc gtcgtatatg tgcatatgcg tgtcacaggt
     1921 accggctggc gccttgagct tcggtataac ggtgctgtcg atggggggca cgacaagggc
     1981 ctaaggacaa atgtagccgg tgccgttcgg attcggaaaa cagccgaccg attgcggcag
     2041 caagtgcttg ggcagccaca gaacgatgtc ggggaagtag attgtgaacg caatggccgc
     2101 gagaaacaca atgtagatgg gcagcgacgc cagcatcgcc ttggagaact ttatgccgac
     2161 gaacttcgat gccatcagca ggcacagccc gtaaggcgg
//
LOCUS       DASSRL010000203         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1125447, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000203 DASSRL010000000
VERSION     DASSRL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125447"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFL49_04215"
     CDS             <1..181
                     /locus_tag="VFL49_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911209.1"
                     /translation="VRDEAARSRIAPELLATRRDLEQLVFAGRSDHLVNGWRGAVIGE
                     RLLALAAEAQSATAG"
     gene            complement(213..1178)
                     /locus_tag="VFL49_04220"
     CDS             complement(213..1178)
                     /locus_tag="VFL49_04220"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7911210.1"
                     /translation="MRIVLLGAPGSGKGTQAQRLQGKYGVPQISSGDLLRDAVARGTE
                     LGLKAKAVMEAGQLVSDDIVLGLIRDRLSRPDAAKGFILDGFPRNKDQADSLNALLNE
                     IGQPLEAVLLLDVRNETLVQRLAGRRICRKCGSVYNVQSLDGATVCPKDGNELYQRPD
                     DKEEVIARRLEVYEEQTRPLIDYYSRLGLLRVIAGEGELAEVSERMEAAALAKPVSSE
                     AVKVEKRAAAKRAPAKRKPAARPAKAKGTSRSAAKSAKKTATKAAKKKAAKKKAVGKA
                     AKKSARGKVARKAAKRRTPAVRRAAKRSAKHVRKAARKTPARRKK"
     gene            1373..2644
                     /locus_tag="VFL49_04225"
     CDS             1373..2644
                     /locus_tag="VFL49_04225"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7911211.1"
                     /translation="MPSKKPRNAFYAQSGGVTAVINASACGVIETCRKHKNKIGKVYA
                     GRHGIVGALTEDLIDTTKESAAAIRALRHTPAGAFGSARYKLKKYEDNPAQYERLIEV
                     FKAHDIGYFFYNGGGDSADTCLKVSLLAEKMGYPIQAVHVPKTVDNDLPITDCCPGFG
                     SVAKYVAVSIREAGFDVASMAKTSTKVFIMEVMGRHAGWIAAAGGLAAEKAGDAPHII
                     LFPEIVFDEEKFCARVKECVEKYGYCAIVVSEGVKNADGKFLSDAGLRDAFGHAQLGG
                     VAPVLAQIVKAKLNYKYHWAVADYLQRAARHIASKVDVDQAYAMGKAAVELALKGHNS
                     VMPTVKRISDKPYKWKVGIAKLSDVANQEKMLPRDYITDDGFFITAKARKYLAPLIAG
                     EDYPPYKNGLPQYVTLKNAPVKKRLNSTFVV"
     gene            2799..>3647
                     /locus_tag="VFL49_04230"
     CDS             2799..>3647
                     /locus_tag="VFL49_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET7911212.1"
                     /translation="MSTDIALWLAIGAGVLAVLYGVFSVGWINRQSAGNERMQEIAAA
                     IQAGAKAYLNRQYSTIAVVGVVLFLLLGFSLGWPTAGGFAVGAVLSGLAGYIGMNVSV
                     RANVRTAQAASVGLNPALQVAFRGGAITGMLVVGLGMIGVAGYFTLMRGSIGDEAALH
                     SLVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGD
                     NVGDCAGMAADLFETYAVTVVATMVLGGLMFQAADGGIDLRYVLYPLVLGGVSIVACI
                     VGSFFVK"
BASE COUNT          654 a         1152 c         1099 g          742 t
ORIGIN      
        1 tgtgcgcgat gaagcagcgc gcagccgcat cgcccccgaa ctgctggcga cgcggcgcga
       61 tctggaacaa ctggtgtttg ccggacgctc cgaccatctg gtcaatggct ggcgtggcgc
      121 ggtgatcgga gaacgcctgc tggcgcttgc cgccgaggca caatccgcaa cggcggggta
      181 acccagccgc gcggatcggg ccagtcagtt cgtcattttt tgcggcgtgc gggcgtcttg
      241 cgagcggcct tgcgaacatg ctttgcagat cgctttgccg cacgtctgac cgcgggcgtc
      301 ctgcgtttcg ctgccttgcg ggcgaccttg ccgcgcgctg actttttcgc cgcttttccg
      361 acggccttct tcttcgcagc cttcttcttt gcagccttgg tagccgtctt tttcgccgac
      421 tttgctgcgg accggctcgt ccctttggcc tttgccggcc tggcggcggg cttgcgtttg
      481 gctggcgccc tcttcgccgc cgcacgcttc tccaccttta cagcctcgct ggaaaccggt
      541 ttggccagcg ctgccgcttc catccgttcc gacacttcag cgagttcacc ctcaccggcg
      601 atgacacgca acaaacccag ccgcgagtaa tagtcgatca gcggacgggt ctgctcttcg
      661 tagacttcca ggcgcctggc gatgacttct tccttgtcgt cagggcgctg atacagctcg
      721 ttgccatcct tcggacatac cgttgcgcca tcgagcgatt ggacgttgta gaccgagccg
      781 cacttgcggc agatccgacg gccggccaga cgctgcacca gcgtctcgtt gcgcacgtcg
      841 agcagcaaca cggcttcgag cggctgaccg atctcgttca acagcgcgtt cagcgagtcg
      901 gcctggtcct tgttgcgcgg gaagccatcc agaatgaaac ccttggcggc atcgggacgg
      961 ctgagacggt cgcggatcag cccgagcacg atgtcatcgg acaccagctg gccagcctcc
     1021 atgacggctt tggccttgag tcccagttcc gtgccacgcg cgaccgcatc gcgcaacagg
     1081 tcgccggatg aaatctgcgg cacgccgtat ttgccctgca ggcgctgtgc ctgcgtgccc
     1141 ttgcccgaac cgggcgcccc cagcagaacg atacgcattg ttttgaacgg cctcccgaaa
     1201 aggaggcgct accctacaga aaaccggaca actggccaac tggctcgccg gaccgccggt
     1261 cgaattcagg aagctgttcc cataccgccc ttgctgccag cgaccatcca gccaaccgcc
     1321 cggctgcgtt atccttcgcg gccgaattcc aaccaacgct caggggcccc gcatgccgtc
     1381 gaagaaaccc agaaatgcct tttatgccca gtccggtggg gtgaccgccg tgatcaacgc
     1441 ctccgcctgc ggtgtcatcg aaacctgccg caagcacaag aacaaaatcg gcaaggtgta
     1501 cgcgggacgt cacggcatcg tcggcgcgtt gaccgaagat ctgatcgaca ccaccaagga
     1561 atccgctgcc gccatccgcg cgctgcgcca caccccggcg ggtgcatttg gttcagctcg
     1621 ctacaaactc aagaaatacg aagacaaccc cgcgcagtac gagcggctca ttgaagtctt
     1681 caaggcgcac gacatcggtt atttcttcta caacggcggc ggtgactccg ccgatacctg
     1741 cctgaaggtt tcgctgctgg cggaaaaaat ggggtatccc attcaggccg tgcatgtgcc
     1801 caagactgta gacaacgacc tgcccatcac cgactgctgc cccggtttcg gttcagttgc
     1861 aaagtatgtg gccgtatcga tccgtgaagc cggcttcgac gttgcttcca tggccaagac
     1921 gtcgaccaag gtcttcatca tggaagtgat gggccgccat gcaggctgga tcgcggccgc
     1981 cggtgggctc gctgcggaaa aggctggcga tgcaccccac atcatcctgt tcccggaaat
     2041 cgtgttcgac gaagaaaaat tctgtgcccg ggtcaaggag tgcgtcgaga aatatggcta
     2101 ctgcgccatc gtcgtttccg aaggcgtgaa gaacgcggat ggcaagttcc tctccgacgc
     2161 gggcctgcgt gatgcattcg gtcacgcgca gctcggcggg gtggcaccgg tcctcgcgca
     2221 gatcgtcaaa gcgaaactca actacaaata tcactgggcc gtagccgact acctgcagcg
     2281 cgccgcccgg cacatcgcct ccaaggtgga tgtggatcag gcttacgcca tgggcaaggc
     2341 cgctgtcgaa ctggcactca agggtcacaa ttcggtcatg ccgaccgtga agagaatttc
     2401 cgacaaaccg tacaagtgga aggtgggtat cgcgaagctc agtgacgtgg cgaatcagga
     2461 aaagatgctg ccgcgtgact acatcaccga cgatggtttc ttcatcacgg cgaaggcacg
     2521 caagtatctg gcgcccctga tcgccggtga ggattacccg ccgtacaaga acggtctgcc
     2581 gcagtacgtg acgctgaaga atgcgcccgt gaagaagagg ttgaattcga cctttgtggt
     2641 atagtccgcc gcctttttcg cggctgtttt cagcaaaaat cgtctgcacg ctgtatgcaa
     2701 cggaccgggt ttgtcgccag gcgatgaacc cggtccgcag cgatttattg ttcggttcac
     2761 aacgaagttt cacaaggtcg tagggagaga ggaagttcat gtccacagat atcgcgctct
     2821 ggctcgcgat cggcgccggt gttctcgctg ttctctatgg cgtcttttcc gtcggctgga
     2881 tcaatcggca atcagcgggc aatgaacgca tgcaggaaat cgcagccgcc atccaggccg
     2941 gcgcgaaggc atatctgaac cggcagtact cgaccattgc cgtcgtgggc gtcgtgctgt
     3001 ttctgctgct cggcttctcg ctgggttggc ccaccgcggg cggcttcgca gtcggggcag
     3061 tcctgtccgg tctcgcggga tacatcggca tgaacgtgtc ggtgcgcgcc aacgtacgca
     3121 ccgcgcaggc cgcgagcgtc ggactcaatc ctgcattgca ggtcgccttc cgcggtggcg
     3181 cgatcaccgg catgctggtg gtgggcctgg gcatgatcgg cgtcgccggt tacttcaccc
     3241 tgatgcgcgg ttccatcggc gacgaagcgg cattgcattc gctcgtgggc ctcgccttcg
     3301 gcggctctct catctcgatc ttcgcgcgac tcggcggcgg catcttcacg aagggcgcag
     3361 acgtcggcgc cgatctcgtc ggcaaggtgg aagccggtat tcccgaggat gatccgcgca
     3421 accctgccgt gattgccgac aacgtcggtg acaacgtggg cgattgcgcc ggcatggcag
     3481 ccgacctgtt cgaaacctat gccgtcaccg tggtcgccac catggtgctg ggcggtctca
     3541 tgttccaggc tgccgacggc ggcatcgatc tgcgttacgt gctctatcca ctggtgctcg
     3601 gcggtgtctc catcgtggcg tgcatcgtcg gttcgttctt cgtcaag
//
LOCUS       DASSRL010000204         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_617850, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000204 DASSRL010000000
VERSION     DASSRL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617850"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFL49_04235"
     CDS             <1..445
                     /locus_tag="VFL49_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     lipoprotein"
                     /protein_id="HET7911213.1"
                     /translation="IDVSVKTAELLGFYGHGVARVKVDYIGRAPLAGSDDRKLMATLR
                     QGHPAQLPVQVASGKPFAPAYFDSRPLRASAQIPSPPDRPYRLGETPSELPAADASTT
                     ELAARPRSPGADAQEPVGPRVSPVSAYAPRVYDMRPGYMSGSGLY"
     gene            521..1780
                     /locus_tag="VFL49_04240"
     CDS             521..1780
                     /locus_tag="VFL49_04240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7911214.1"
                     /translation="MGIRNLAFGRWLTVLALAGSLSAAAIAAPPAHKGAHAPAQKKDA
                     PKKEAPKKDEPNVSAPHAILIDAEYGGVLFDRDADRLIFPASLAKLMTAEYVFHEVKE
                     GRIKLTDEYTVSENAWRKGGAPSHGSTMFAALNSKVSVDDLLHGMIIQSANDACIVLA
                     EGIAGNEAEFGSKLTQRAREIGLEKSVFTNSNGLPDPNEKVTTRELAILARHIIRTYP
                     DFYQLFSQPDFTWNKIRQQNRNPLLGAMNGADGLKTGYTKEAGYGLVGSAVQNGLRLI
                     VVVTGVSTAKERADEAKKVLEWGFRNFEQRTIFADGQTIGAAKVFGGSSGRVALRADG
                     EVKVMLPKSGADKLVARIVYTGPVPAPVAEGTPIGNLKVWRNDNLILTMPLKAAETVG
                     KGNMPQRAFDAVTEMIIALFRAGAERL"
     gene            1830..2495
                     /gene="tmk"
                     /locus_tag="VFL49_04245"
     CDS             1830..2495
                     /gene="tmk"
                     /locus_tag="VFL49_04245"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7911215.1"
                     /translation="MRGHFITFEGGEGSGKSTHAATLAQRLKTAGQEVVLTREPGGST
                     GAEIIRHILLSGIAKPLGAETEAILFAAARDDHVRSTILPALKSGTWVICDRFIDSTR
                     VYQGALGNVDERLIRSLERVTVGPAVPELTFILDVPAQVGMARAKSRRGQVEADRFEA
                     ESMEFHEKLRGAYRALAAAEPKRCIVIDGRAPREVVADRIWSIVQERLRPQRLGIAER
                     SAP"
     gene            2492..>2720
                     /locus_tag="VFL49_04250"
     CDS             2492..>2720
                     /locus_tag="VFL49_04250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664280.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the delta' subunit seems to
                     interact with the gamma subunit to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7911216.1"
                     /translation="MSRDEDEDNGAKSPRETTVLFGHADAEKALLDAYKGGRIAHAWL
                     IGGPPGIGKATLAYRMARFVLANPDPQSPAVQ"
BASE COUNT          545 a          836 c          867 g          472 t
ORIGIN      
        1 gattgacgtt tcagtcaaga ccgcggagct gctgggtttc tatggtcacg gcgttgcgcg
       61 ggtgaaggtt gattatatcg gccgcgctcc tttggcaggc tccgacgacc ggaagctgat
      121 ggcgacgttg cggcaaggcc acccggcgca actgccggtg caggtcgcat ccggcaaacc
      181 gtttgcaccg gcgtatttcg atagccggcc gctgcgcgcg tcagctcaga tcccgtcacc
      241 tcctgaccgg ccttatcgtc tgggcgagac accgagcgaa ttgccggcgg cggacgcttc
      301 gaccaccgaa cttgctgcac ggccccgctc gccgggcgcg gacgcgcaag aacctgtggg
      361 acctcgcgtg tcaccggttt ccgcctatgc ccctcgggtc tacgatatgc gaccgggcta
      421 tatgagcggc agcggccttt actgagccgc attctaccgt caaaggtcgc ctgatcgggc
      481 cgccccgatt ttgccgggtg ccgccaaaag agccttgaaa atgggcatac gcaatctagc
      541 gttcggacgg tggctgacgg tgctggcgct tgccggcagc ctttctgcgg cagccattgc
      601 ggcaccgccg gcgcacaagg gcgcacatgc ccctgcgcaa aagaaggacg caccaaaaaa
      661 agaggcgccg aagaaagacg agccgaatgt cagcgcgccg cacgcgatcc tgatcgatgc
      721 cgagtacggc ggcgtgctgt tcgatcgcga tgccgaccgc ctgatctttc cggcaagtct
      781 cgccaagctc atgaccgccg agtacgtctt tcatgaggtc aaggaagggc gcatcaagct
      841 caccgacgaa tacacggtga gcgagaacgc ctggcggaaa ggcggcgcgc cttcgcacgg
      901 ctccacaatg tttgcggcgc tcaacagcaa ggtttcggtg gacgacctgc tgcacggaat
      961 gatcatccag tccgcgaatg atgcctgcat cgtgctcgcg gaggggattg ccggcaacga
     1021 agctgaattt ggcagcaagc ttacgcaacg cgctcgcgag atcgggctcg agaaatcggt
     1081 tttcaccaac tccaacggcc ttccggatcc gaacgagaag gtgacaacgc gcgaactcgc
     1141 gatcctcgcg cgtcacatca tccggaccta tccggacttc tatcagctgt tttcccagcc
     1201 ggatttcacc tggaacaaga tccgccagca gaatcgcaat ccgcttctgg gtgcgatgaa
     1261 cggcgccgac ggcctgaaga ccggttacac aaaggaggcg ggttacgggc tcgtcggctc
     1321 ggcggttcag aacgggctgc ggctgatcgt ggtcgtcacc ggcgtgagca ctgccaagga
     1381 acgtgccgac gaagccaaga aagtcctgga gtggggtttc aggaatttcg agcagcgaac
     1441 catctttgct gacggccaga cgatcggtgc cgcgaaagtc ttcggcggct ccagcggccg
     1501 tgtcgcgctg cgtgccgatg gcgaagtcaa ggtgatgctg cccaaatccg gggcggacaa
     1561 gctcgttgcc cgcatcgtct acaccggacc ggtgccggct ccggtagccg aaggcacacc
     1621 gatcggaaat ctcaaggtct ggcgtaacga taacctgatc ctgacgatgc cgctgaaagc
     1681 cgctgaaacc gtcggcaagg gaaacatgcc gcagcgcgca ttcgatgctg tgactgaaat
     1741 gataatcgcg ctgttccgtg ccggagccga gcggctatga tgtcggcagc caccgtatgc
     1801 cggtggtgac cggaaaaggc tgatcgccat tgcgtggaca cttcatcacc ttcgaaggcg
     1861 gcgaaggcag cggaaaatcc acccacgcag ccacactggc ccagaggctc aagacggcag
     1921 gacaggaggt cgtgctcacg cgcgagccgg gcggctcaac cggggccgag atcatccgcc
     1981 acatcctgtt gtccggcatc gcaaaaccgc ttggcgcaga aaccgaagcg atccttttcg
     2041 ctgccgcacg cgacgatcac gtgcgcagca cgattttacc ggccctgaag tccgggacct
     2101 gggtcatctg cgaccgcttt atcgattcaa cccgggtgta tcagggcgcg ctcggcaatg
     2161 tcgatgagcg gctgatccgc agccttgaac gtgtcaccgt cgggccggct gtgcccgaac
     2221 tgacgtttat tctcgacgtt cccgcccaag tcgggatggc aagggcaaaa agcagacgcg
     2281 gccaggtcga ggctgaccgg tttgaggcgg agtcgatgga gtttcacgag aaactgcgcg
     2341 gggcttaccg ggcgctcgca gcggcggaac cgaagcgctg catcgtcatc gatggccgcg
     2401 cgccgcgcga ggtcgtcgcg gaccggatct ggtcgatcgt gcaagaacgg ttgcgcccgc
     2461 aacgtctcgg aattgcggag aggtcggcgc catgagccgg gacgaggatg aggacaatgg
     2521 cgcaaaatcg ccccgtgaga caactgtgct attcggccac gccgacgcgg aaaaagcgct
     2581 cctcgatgcc tacaagggtg gacggatagc ccatgcctgg ctgatcggcg gtccgcccgg
     2641 catcggcaag gcgaccttgg cctatcgcat ggcgcgtttt gtgctggcca atcccgatcc
     2701 ccaatctcct gccgtgcaga
//
LOCUS       DASSRL010000205         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_460487, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000205 DASSRL010000000
VERSION     DASSRL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460487"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFL49_04255"
     CDS             complement(<1..180)
                     /locus_tag="VFL49_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911217.1"
                     /translation="MKAPNRKESAGNGCNARCRLYGPTAELHDDKGEIQAAHRDKQLV
                     THKPPPEQFRRRHQDR"
     gene            complement(181..1188)
                     /locus_tag="VFL49_04260"
     CDS             complement(181..1188)
                     /locus_tag="VFL49_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET7911218.1"
                     /translation="MTFGPGGLIFAAAAALSYGLLLGLMPLLTRYAVAKPNARSSHRT
                     PTPQGGGIAVIAATVIAGAAAAMILQANEMPALAIVFLAAAGLALVGALDDRRPMEPA
                     PRLLLQAVAVIAVIAALPPEMKIVPASPWWLDRMVMLVGGIWLINVVNFMDGIDWMTV
                     AEIVPVTAGLAVLGWMGALPASAAIVAAALCGAMVGFAPFNKPIARLFLGDVGSLPVG
                     LLLGWMLVLLAGRGHLAAALLLPLYYLADATITLIRRTARGERITQAHRSHFYQRALD
                     GGYSVYQIVGRVFALNILLAALAVATIVKSSSALGVICLLAGIVLVGLALWSFARAAS
                     K"
     gene            complement(1218..2357)
                     /locus_tag="VFL49_04265"
     CDS             complement(1218..2357)
                     /locus_tag="VFL49_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7911219.1"
                     /translation="MARKILYLVSEDWYFVSHRLPMARAAQSAGYEVHVATRVGDCAE
                     DIRREGFHLHPIQWRRGNMNPLRLLAAALETRGIYRQINPDLAHHVALVPTLIGSLAA
                     IGLPVVRLNAIAGLGFAFTSDTAKARAVRPLASLLLGWLLKRPHTTVLVQNPDDYAVV
                     AKLGVRKDNIALIPGSGVDTDLLTPMPEPAGPFTIAFVGRLLDDKGVRTLVRAHELLA
                     QRGLTVRTLLAGEPDPSNPASIDERVLANWRRIPNLKLLGHVDDIGTVWVQAHVAVLP
                     SRREGLPKSLLEAAACGRPLIATDVPGCREVARSDVNAVLVPPDDAKALADAIETLLK
                     DPSMRKRFASASRRIAVSEYSNERIAAEITRLYSRLLQQAPSSTL"
BASE COUNT          491 a          755 c          773 g          417 t
ORIGIN      
        1 tctgtcctga tgtcggcgac ggaactgctc tggcggtggc ttgtgcgtga cgagctgctt
       61 gtcgcgatga gcggcctgta tctcgccctt atcgtcatgc aattcagcgg tggggccata
      121 cagtcggcaa cgagcattgc agccgttacc ggcgctttcc ttgcggtttg gggccttcat
      181 ttatttactg gcagcacgcg caaaactcca cagcgcaagg ccaaccagta cgataccggc
      241 gagcaggcat atcaccccca gcgcggatga gcttttgacg attgtcgcga cagccagtgc
      301 ggcaagcagg atattcagcg caaagacccg gccgacgatc tgataaacgc tgtacccccc
      361 gtccagtgcc cgttgataga agtggctgcg atgcgcctgt gtgatccgct cgcctcttgc
      421 ggtgcggcgt atcaaggtga ttgtcgcgtc ggcgaggtag tagagcggca acagaagtgc
      481 tgcggcgagg tggcctcgac cagcgagtag caccagcatc cagccgagca ataacccgac
      541 gggaagactg ccgacatcgc ccagaaacag gcgcgcgatc ggcttgttga acggcgcaaa
      601 gcccaccatg gcgccgcaaa gtgcggcagc aacgatagct gcggacgccg gaagggcgcc
      661 catccacccg agcacagcga ggcctgccgt caccggcacg atttcggcga cggtcatcca
      721 gtcgatgccg tccatgaaat tcacgacgtt gatcagccag attccgccga cgagcatgac
      781 catgcggtcg agccaccagg gcgacgccgg cacaattttc atttcgggcg gaagggccgc
      841 aatgaccgcg atgacggcaa ccgcctgcag aagcaggcga ggggctggct ccatgggccg
      901 ccggtcgtca agcgccccca cgagcgccag tccggcggcc gccaggaaca cgatcgcaag
      961 tgcaggcatt tcattggcct gcagaatcat cgccgctgct gcgccagcga taaccgtcgc
     1021 agcgatgacg gcaatcccgc caccctgcgg ggtcggcgtc ctgtgcgaag agcgcgcatt
     1081 gggcttggcc accgcgtaac gggtgagcaa cggcatcagg cccagcagga ggccgtaact
     1141 cagcgcggca gccgcggcaa atatcagtcc acccggtccg aatgtcacgc cgtcaccacg
     1201 atgtctctgg gcttgcctta gagtgttgac gatggcgcct gctgcagcaa gcgcgaataa
     1261 aggcgtgtga tttccgccgc gatacgctca ttcgagtatt cgctgaccgc gatcctgcga
     1321 ctggcgctgg caaaccgttt ccgcatcgag ggatccttca acagcgtttc aatcgcatct
     1381 gcgagggcct tcgcatcgtc gggtggaacg agcaccgcgt tcacgtcgct gcgtgcaact
     1441 tcccggcagc cgggaacgtc cgtggcgatc aggggccggc cacatgcggc tgcttcgagc
     1501 aagctttttg gcaagccttc ccgtcgcgaa ggaaggaccg cgacgtgcgc ttgcacccac
     1561 accgtgccta tgtcgtcgac atgaccgagc agcttgagat tgggaatccg ccgccaattc
     1621 gccagaacac gttcgtcgat cgacgcggga ttggatggat cgggctcgcc cgcaagcaaa
     1681 gtacgaaccg ttaatccgcg ctgcgcgaga agttcatggg cgcggaccag cgtgcgtacg
     1741 cctttgtcgt caagcaggcg tccgacaaag gcgatcgtga acggaccggc cggctccggc
     1801 attggcgtca gcaaatcggt atcgacgccg gagccgggga ttagcgcgat attgtctttc
     1861 cgcacgccga gtttcgcgac gactgcatag tcgtccggat tctgcacgag cacggtggtg
     1921 tgaggccgtt tcagcagcca gccgagaagc aggctggcaa gcggccgcac ggcgcgcgcc
     1981 tttgcggtgt cggaggtgaa tgcaaagcca agtcctgcaa tggcattgag tctgacaacg
     2041 ggaagtccga tcgccgccag agaaccgatc aaggtcggaa cgagcgcgac atggtgggca
     2101 agatccggat tgatctgccg gtagatgccc ctggtttcga gtgccgcagc aagcagccga
     2161 agcgggttca tgttgccgcg acgccattga atgggatgca ggtgaaatcc ctcgcgccga
     2221 atgtcttcgg cgcaatcgcc gacgcgagtc gcgacgtgga cctcataccc ggcgctttgc
     2281 gcggcgcggg ccatcggcag ccggtgcgac acgaagtacc agtcttcgga aaccagatag
     2341 aggatcttgc gcgccatcaa acacgtccgc ccagcgatcc gggccgcctc gaacggccgc
     2401 gagcgacctg aacatcgcgg cctacgcaag cgatac
//
LOCUS       DASSRL010000206         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1177852, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000206 DASSRL010000000
VERSION     DASSRL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177852"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1600
                     /locus_tag="VFL49_04270"
     CDS             83..1600
                     /locus_tag="VFL49_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7911220.1"
                     /translation="MRKFAVAVLLALATGAGVLTPAYGQSTVPPGIPPPPTIVNRGAA
                     ITPLGWYVIGSIGCAAVSPIIGTLILGRELTLNEAYRSTFGCVLGPVGWLLADALVPP
                     TPPPPITIQPRSAKTPPQHRRSTGRGRNFKIPPQGETRFVANEILLEFAPGATAQMRN
                     ALASNLQLTELETQRFELTARLLSRWRIDGTRSVPATLRLIARNYPAVSGGWANMIYV
                     GAQIQAPATQPPPAAGSAQYVVNKLHLLEAHRISNGDDVLVAVIDSKIDTKHPDLVGV
                     VANEYDVVGAPGAAHSHGTAMAGAIAARSKLVGVAPKVKLLAVRAFSGSGESAESTTL
                     NILKSVDWAASKNARIINMSFAGPPDELLREMLAKANARGIVLIAAVGNAGPRSPPLY
                     PAADPGVIGVTATDVDDRLMPQANRGPQVAVAAPGVEILAVAPDGKYQVTSGTSVAAA
                     HASGVAALLLASKPSLTPAQVRARLIHGANRIPGKRTEVGAGVIDALAVINPNHK"
     gene            1676..2455
                     /locus_tag="VFL49_04275"
     CDS             1676..2455
                     /locus_tag="VFL49_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7911221.1"
                     /translation="MAVPESLVVERPSSDIVVLRINRPQVRNALNLDVRTRLADEVTR
                     CGADNAVRCVIITGSDVAFAAGADIGEMAEAGPVEVMSRNVQKYWRAIMDCPKPIIAA
                     VEGFALGGGLELALCADIIVAGEGAKLGLPEVKVGILPGGGGTQKLARLVGRHRAMLL
                     IMTGKMFTAPEALDMGVVSEVAPAGQALQRALEIAGEIAAMPAVSIMQIKEIINAGLN
                     APLDTALMLERKAFQLQFATRDQKEGMRAFMEKRKPRFEGQ"
     gene            2459..>2809
                     /locus_tag="VFL49_04280"
     CDS             2459..>2809
                     /locus_tag="VFL49_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7911222.1"
                     /translation="MALDLSRPDLVAAVFGTGTMGRGIAQLCAQAGLTTLLFDSNSVA
                     VKEAVAAIDNGLEGLVSKGRMTAGTKKDVLGRLKPISAYDECAKAGIVIEAIVEDLAA
                     KRALFREIEKRVAAD"
BASE COUNT          532 a          861 c          904 g          512 t
ORIGIN      
        1 tttcacttcg gcccctgaac ctgacgggca tggtgcacga ccaatggccg tgtagaatgc
       61 tttttggaag ctgaggggag ccgtgaggaa gtttgcagta gccgtgttgc ttgcgcttgc
      121 gaccggtgca ggtgtgctga cacctgcgta cgggcaatcg accgtccccc ccggcattcc
      181 gcccccgccg acgattgtta accgtggcgc ggcgatcact ccgcttggct ggtacgtcat
      241 aggttcgatc ggttgcgctg cagtatcgcc gatcatcggc acgctcatat tgggccgcga
      301 actcacgctc aacgaggcct accgttcgac gttcggatgc gtccttgggc ctgtcggctg
      361 gcttttggcc gatgcgcttg tgccgcccac gccgccgcct ccgatcacga ttcaaccccg
      421 gtcagcgaag acaccgccac agcaccgacg ctccacgggc cgtgggcgga atttcaagat
      481 accgccgcag ggcgaaacgc gatttgtggc caacgagata ctgctcgagt tcgcgcccgg
      541 tgcgaccgcg cagatgcgca acgcgctcgc aagcaatctg caattgaccg aactggagac
      601 gcagagattc gaattgaccg cgcgcttgct cagccgctgg cgcattgatg gtacgcgatc
      661 ggtgccggca acgctgcgtc tgattgcccg caactatccg gccgtgtcag gtggctgggc
      721 caacatgatt tatgtcggtg cgcagatcca ggccccggca actcagccgc cgcccgccgc
      781 gggctccgcg cagtacgtgg tgaacaagct gcatctgctg gaagcacatc gcatcagcaa
      841 cggcgacgac gtgctggttg cggtgatcga ttcaaaaatc gacacgaagc atcctgacct
      901 tgttggcgtg gttgcgaacg agtacgacgt cgtcggcgcg ccgggcgccg cccattcgca
      961 tggcacggcc atggccggag caatcgccgc gcgcagcaag ctggtgggtg ttgcgccgaa
     1021 agtgaaactg cttgccgtgc gtgccttttc cggctccgga gaaagcgcgg aaagcacgac
     1081 cctcaatatt ctcaaaagcg tcgactgggc tgcgagcaaa aatgcccgca tcatcaacat
     1141 gagttttgcg ggaccgcctg acgagctatt gcgggaaatg ctcgccaagg caaacgcccg
     1201 gggtatcgtg ctgattgccg ccgtcggcaa tgccggtcca cgctcgccgc cgctctatcc
     1261 ggccgccgac cccggcgtca tcggcgtgac ggcgaccgac gtcgacgaca gactgatgcc
     1321 gcaggccaac cggggccctc aggttgcggt tgcggcgcca ggtgtagaga ttctcgctgt
     1381 cgcgcccgat ggcaagtacc aggtaacctc gggcacctct gtcgcggcag cacatgccag
     1441 cggggttgcg gcgttgctgc tggcgtcgaa gccgagcctg acgcccgcac aggtgcgtgc
     1501 aaggctgatt cacggcgcga accggattcc gggcaagcgc accgaggtcg gagccggggt
     1561 tatcgacgcg cttgccgtga tcaatccaaa tcacaaatag tcttgccgca aatagccttt
     1621 tcgcccggcc atctgctagg taaggccctg aagacagtct tctggatcga ttgccatggc
     1681 cgtgccggag tccctcgtcg tcgaacgacc gtccagcgac attgtcgttc tcaggatcaa
     1741 ccgtccgcag gtgcgcaacg cgctcaatct ggacgtgcgc acgcggcttg ccgacgaagt
     1801 cacgcgctgc ggagctgaca atgccgtgcg ctgcgtcatc atcaccggat cggacgtcgc
     1861 tttcgccgcc ggtgccgaca tcggggaaat ggcggaagcc ggtccggtcg aggtcatgtc
     1921 gcgcaatgtt cagaaatact ggcgcgcgat catggattgc ccgaagccga tcatcgccgc
     1981 cgtcgaaggc tttgcgcttg gcggcggact tgagttggcg ctgtgcgccg acatcattgt
     2041 cgcaggcgag ggtgcaaagc tcggtcttcc agaggtcaag gtcggtatcc tcccgggcgg
     2101 tggcggcacg cagaaattgg cgcggcttgt cggccgccat cgagcgatgt tgctgatcat
     2161 gacaggaaaa atgttcaccg cccccgaggc gctggatatg ggcgtggtca gcgaggtcgc
     2221 gccggcgggt caggcgctgc agcgcgcgct ggaaattgcc ggcgaaattg ccgcgatgcc
     2281 cgcggtgtcc atcatgcaga tcaaggaaat catcaatgcc gggctcaatg cgccgctcga
     2341 tacggcgctg atgctggagc gcaaggcatt ccagctgcaa ttcgccacgc gcgatcagaa
     2401 agaaggcatg cgagccttca tggaaaaaag aaaacccagg ttcgaagggc agtagcgcgt
     2461 ggcgttggat ctatcccggc ccgatcttgt tgccgcggtg ttcggcaccg gcaccatggg
     2521 ccgcggcatc gcccagctgt gcgcgcaagc cggactcacg accttgttgt tcgattcaaa
     2581 cagcgtcgcg gtaaaggaag cggtcgctgc gatcgacaac ggcctcgaag gtttggtgtc
     2641 gaaaggccgc atgactgccg ggacaaagaa ggatgtgctc ggccggctga agccaatcag
     2701 cgcctatgac gagtgcgcaa aggcggggat cgtcatcgag gcaatcgtcg aagatcttgc
     2761 ggccaagcgc gcgctctttc gcgagatcga aaaacgcgtg gccgccgac
//
LOCUS       DASSRL010000207         5757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1370375, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000207 DASSRL010000000
VERSION     DASSRL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370375"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFL49_04285"
     CDS             complement(<1..267)
                     /locus_tag="VFL49_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911223.1"
                     /translation="MRQVSILVFAGVLFTTASSVHSLEASCDFRKCMSICRSDYESGC
                     AGMCGRIISLCKQLVLRPERTSSVRRSSREGIKLHARARGSYGRI"
     gene            complement(457..1500)
                     /locus_tag="VFL49_04290"
     CDS             complement(457..1500)
                     /locus_tag="VFL49_04290"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7911224.1"
                     /translation="MSPVPGGRKARVWMGDEMMDQSGLTTVRFSTADHPEKDRMAMWR
                     DHCCRLVMKLDIEPINGGQLEYSLLARELPGVRVMSTAMSPVRMTRTREYAADGNGDL
                     IFIINQTGKAMASARGRDVMLNEGDALLMSASEAKTFDRHSYGGSLSFRIPRPILSSM
                     VSYVDDVVMQAIPHDTDALKLLAGYAAPLFNDLALAAPDFRRTAVNHLHDLVALALNA
                     ARGGVAAGRAVPAARLRMAKSYIMENSSRRDLSVGGVAAHLGLTPRNLQRLFESEGTT
                     FSEYLLSQRLSRAHRMLTEPRLAQNPVGAIAYDAGFGDLSYFNRSFKRRFGMTPRDVR
                     NEGVKFLELPDIR"
     gene            1983..2903
                     /locus_tag="VFL49_04295"
     CDS             1983..2903
                     /locus_tag="VFL49_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7911225.1"
                     /translation="MTFWSTDTIPQRERFSFWREVICKTIFSISSEAPSECFSARVRV
                     RSSGSLRFAMCQSTSYEIIRTQRDVNRAPADYYTVYLQLQGRTLISQCDESAALDCND
                     IVLSDCRRPYSATLSNDGLRAVAVLPRPMVNSRAPWLSERPLHKIPPTSQYLDLARRH
                     MIQLMSEKLGDRESDLLTENLCNLLALASAPDIPPSRLRTELQTAAMLAFCRQNLHRP
                     DLSPQMVASHFVVSLRTVHSRFERLGTTFGRWLLETRLETCSKALKGPRQPRYISEIA
                     YNCGFNDLSHFNKAFRARFQMAPTEWRDLS"
     gene            2911..3870
                     /locus_tag="VFL49_04300"
     CDS             2911..3870
                     /locus_tag="VFL49_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7911226.1"
                     /translation="MKPAIGTTTWSTDSIDERERFSFWREMVCRNVFNISVQAPPERF
                     FASITARSHGSLRFAMCESSAYETDRTRRDIANESEDHYLMYLQLRGHTLVTQPDDSI
                     EFGRNDIVIADGRRPYHADLFDDGHCRRQAIAVLPRSVIEERAPWLRRRPLFKVSSHS
                     IYVDLTRRHMLQLAADDLTERELALLTENLCNLLVLLDHAEIPINRVQPELQLEALLD
                     FCRRNLHDPALSADFVAAQFGLSTRALHLRFEKLEQTFGRWVLDARLDACSTALRDPL
                     QQPRAISEIAFSCGFSDLSHFNKSFRARFGMPPSEWRAEISRR"
     gene            4437..>5757
                     /locus_tag="VFL49_04305"
     CDS             4437..>5757
                     /locus_tag="VFL49_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911227.1"
                     /translation="MEALIAAICGVNILVSPAFAEDKTDASAQLVAPRQVLTQRAPAQ
                     QEPSPAPARSSPAQQMPARQDRLPQSPPQDSLRQRWSTTFSSEIRYFSWRNDFSPPDT
                     SGIGPGRGWEVYIPFAMQLTGKPVDDLSVDFVARGGWVKAAQTTTGLSGEVATSTDTV
                     MSVNAIYLGWQGIQPFVALSTNLPTGKSALFGTQVNARMDPDLVEISTFGEGFNLGPS
                     FGLNIPIAGSLLITTSVGYTWRDAFKRESAFPVFPPGVNPALVANLIDIIPTATINPG
                     ENITATAAINYGMGSFAIGMTGSASWESPTIITDTPIFRPGLRYLLALQSSYKWPENW
                     GITELTAAVAHSNRNKVLRREATELALEDLNSNSNVYRIGLQHLFPVAQFQVGPTGSV
                     LYRDHNGYNATTLQFVPQKKRWAAGMVAQYVPNERITINARVEHVWIH"
BASE COUNT         1220 a         1764 c         1599 g         1174 t
ORIGIN      
        1 gatacgacca taagagcctc gggcccgagc atggagcttg atgccttcac gcgatgagcg
       61 ccgcacactg ctcgtgcgct ccggtctcaa caccagctgc ttgcagagcg aaatgatcct
      121 gccgcacatg ccggcacagc ccgattcata gtcgctccgg catatgctca tgcatttgcg
      181 aaaatcgcag ctggcttcca ggctgtgcac cgaactggca gtggtgaaaa gcacacctgc
      241 aaagacaaga atggaaacct gacgcatgaa aacgaatctc ccgcaaaatc gggacgtctc
      301 atcgcgcatt gcgtgatgct tcgcgtcatc cgcttgcagg tgtgcggtgg ttatgatgaa
      361 cgcaccgaat tactctttaa tcggcgcgta atcgcctggc ccttacgaca gggcgcggag
      421 tggaaacgaa caaggtaaat tggtcctgct ggccggttac cgaatatccg gcagctccag
      481 aaattttacg ccttcattcc gcacatcccg gggggtcatt ccaaagcgtc gcttaaacga
      541 acgattgaaa taggacaaat ccccaaagcc tgcatcatat gcgattgcac cgaccggatt
      601 ttgcgccagc cgcggctcgg tcaacatgcg gtgggcgcgg ctgaggcgct gtgacagcag
      661 atactccgag aatgtcgtgc cttcgctttc aaacagccgc tgcagattgc gcggcgtcag
      721 cccaagatgg gctgcgactc cacccaccga gaggtcgcgg cggctgctgt tttccatgat
      781 gtatgatttt gccattcgca atcgggccgc gggaactgca cgccctgccg ccacgccgcc
      841 acgcgccgcg ttaagcgcaa gcgcaacgag atcatgcaga tgattgacgg cggtacgccg
      901 aaaatccggc gctgccagcg cgagatcatt gaacagcggc gccgcataac ctgcgagcaa
      961 tttcagcgca tcagtgtcgt gcggaatagc ctgcatcacg acatcgtcga catagctgac
     1021 catcgacgac aagatcggcc gcggaatccg gaatgaaaga gaacctccat atgagtggcg
     1081 atcaaaggtc tttgcctcgc ttgcgctcat cagcagagca tcgccttcgt tcagcataac
     1141 atcccggcca cgggcggagg ccatggcctt gccggtctga ttgataataa aaatgaggtc
     1201 tccgttgcca tcggccgcgt attcgcgcgt tcgcgtcatg cggaccgggg acattgcggt
     1261 cgacatcacc cgcacgccgg gaagctcacg agccagtaac gaatattcca gctgtccgcc
     1321 attgatcggc tcgatatcga gcttcatcac cagacggcag caatggtcac gccacatcgc
     1381 catacgatcc ttttccggat ggtcggcggt agaaaaccga acggtggtaa ggccagattg
     1441 atccatcatc tcatcgccca tccaaacccg cgccttgcgg cctcccggaa ccggggacat
     1501 ttcgacaaat ttgacgaaga gacgattgtt caccggtgat aggcgcacga aatcgccgcg
     1561 gccgggcgaa gtcggcgctc ctacgaaggt ggccaggcac aatcaagttt gaacattcgt
     1621 cgccctactc tctctggtcg caagatgcca gcaaatacac taatacgcgt cctccgaatg
     1681 gaggacgagt ttgggtagtc gcgcttctcc gcttggcatc tcaaaggaaa cgtaaaacac
     1741 atcgcgcaaa ctcagcgcgt tcccgcaggg aagtcgcaag ccgagccaag cggcaacgac
     1801 aaatgccaga gtttcgtcgt gtgtcccgat tgctcctctt cttaagactt gctgtgaccg
     1861 gcaggccgga tgtatacgca cgcaaccaca agcagtcttg gcttgcggcg caatcggctt
     1921 ggttgagagt gcaggcatag ttatccgggc gtttccgcga tatttgttcg cggggggcag
     1981 gcatgacttt ctggtcaacg gacacgatac cgcagcgcga acgcttctcg ttctggcgcg
     2041 aagttatttg caagacaatc ttcagcatct cgtctgaagc gccgagcgag tgcttctcgg
     2101 cgcgcgtgcg cgtgcgcagc tccggttctc tgcgctttgc catgtgccaa tccaccagct
     2161 acgaaatcat ccgcacacaa cgtgacgtca accgggctcc cgcggattac tacacggttt
     2221 acctgcagtt gcagggccgc acgctgatca gccagtgtga cgaatccgca gcgcttgatt
     2281 gcaacgacat tgttctttcc gactgccggc gtccctacag cgctacgctg tccaacgacg
     2341 ggctgcgcgc ggtcgccgtt ctgccgcgcc cgatggtcaa ttcgcgcgcg ccctggctga
     2401 gcgagcggcc cctccacaag attcctccga catcacaata tctcgacctc gcacggcgtc
     2461 acatgattca gctgatgtcc gaaaagctcg gcgaccgcga gtccgatctg ctgacggaaa
     2521 atctctgcaa tctgctggcg ctggccagcg cgcccgatat tccaccgagc cgcctgcgga
     2581 ccgagctgca gaccgcggcc atgctcgcgt tctgccggca gaacctgcat cggcccgact
     2641 tgtctccgca gatggtagcc tcgcactttg tcgtgtcgct cagaacggtt catagccgct
     2701 ttgaaagact gggcaccacc ttcggacgct ggctgctcga aacccggctg gagacctgct
     2761 cgaaggcgct gaaaggcccg cgccagccgc gctacatttc cgagatcgcc tataattgcg
     2821 gcttcaacga cctgtcgcat ttcaacaagg cgttccgtgc ccgctttcaa atggcgccca
     2881 ccgaatggcg cgacttgtcg tgaagaacca atgaagcccg cgatcggcac gacgacttgg
     2941 tcaaccgact cgatcgacga gcgcgaacga ttctcgttct ggcgcgaaat ggtttgccgg
     3001 aatgtcttca atatctcggt gcaggccccg cctgaacgat tcttcgccag catcacagcg
     3061 cgcagtcacg gatcgcttcg gttcgccatg tgcgaatcca gcgcgtatga aaccgaccgc
     3121 actcggaggg acattgccaa cgagtcggag gatcactatt tgatgtacct gcaactgcgc
     3181 gggcacacgc tcgtcacgca gcccgatgat tcgattgaat tcggccgtaa cgacatcgtc
     3241 atcgccgatg gacgacgccc ctatcacgcc gacctttttg atgatgggca ttgtcgccgc
     3301 caggctatcg ccgtgctgcc gcggtccgtc attgaagagc gcgcgccgtg gctgcgccgg
     3361 cggcccctgt tcaaggtctc ctcacattcg atctatgtgg accttacccg ccgccacatg
     3421 ctgcagctcg ctgccgacga cctgaccgag cgcgaattgg cgctgctgac cgaaaatctc
     3481 tgcaatctgc tggtactcct cgatcatgct gagatcccga tcaatcgtgt gcagcccgaa
     3541 ttgcagctcg aagcgctgct ggatttctgc cggcgcaatc tgcatgaccc cgcgttgtct
     3601 gccgattttg tggcggcgca gttcggactc tcgacgcgag ccctgcatct gcgcttcgag
     3661 aagttggagc aaacattcgg ccggtgggtg cttgatgcgc ggctggatgc gtgcagtacc
     3721 gcgcttcgcg atccgttgca gcagccgcgt gcgatctctg aaatcgcatt cagctgcggc
     3781 ttcagcgatc tttcacattt caacaagtca tttcgcgcgc gctttgggat gccgccgagc
     3841 gaatggcgcg ccgaaatttc gaggcgataa gtcgtcgtcc aagcaccggg ctggtcgcgc
     3901 ctcgcggtcc ctcaagccgc gctattgctg ctggcgtttg ttgttattcg gtgcggaacc
     3961 cggaatgatc acaggtttag cgatgccgac agcgttcggc ggcggagggg acgcaatttt
     4021 tttcagcaga gacgggttgc gcaaaagcca gaacgctcac gagaccgagc agcatggcga
     4081 aaaatatttg catcccatct ctcctatcag acgcaccacc gcgcctcaga accatcgtga
     4141 cgatcggcaa tttctacgcg cgtgtaactg tctctgaacg acaccatgcg taaagcgaca
     4201 atgtgtgtgc tcagaacgtg cgcataccaa ggggcaatta catctgaaat tcggtatccg
     4261 agagatgcat ccattacgcg gtgaatcaat tttccggcga tgcgtcgtgc ggaccagact
     4321 aaaaatgctt acgaatatat ttctgactcc gctgcaacct cgttcgagtg aacgacgcgc
     4381 gttgcgttca ttttcgctag tcaactccgc aagctcatgc cggagtgacc ggataaatgg
     4441 aagcgctcat tgccgccata tgcggcgtca acattctcgt ctcgccagcg tttgccgagg
     4501 acaagacgga tgcatcggcg caactggtcg cgccaaggca agtgctgaca cagcgcgctc
     4561 cggcgcagca agagccttca ccggctccgg cgagatcgtc tccggcgcag cagatgccgg
     4621 cgcgacaaga ccggttaccg caatctcctc cgcaagacag tttgcggcaa cgctggagca
     4681 caacgttttc gagcgaaatc cgctattttt cctggcgcaa tgacttttct cctcccgaca
     4741 cctcgggcat cggaccgggt cgcggatggg aggtctacat ccctttcgcc atgcagctca
     4801 ccggcaaacc ggttgacgat ctgagcgtag attttgtggc gcgcggcggt tgggtgaaag
     4861 ctgcgcagac gacgaccggc ctttccggtg aggttgcaac ctctacggac acggtcatgt
     4921 ccgttaatgc gatctatctc ggctggcagg gcatccaacc tttcgtcgcc ctcagcacaa
     4981 acctgccgac gggaaaatca gccttgttcg gcactcaggt caatgcgcgc atggacccgg
     5041 acctggtcga aatctccacc ttcggcgaag gcttcaacct cggcccgtcc tttgggttaa
     5101 acatcccgat cgccggctcg ctgctgatta cgaccagcgt cggttacacc tggcgtgacg
     5161 cgttcaagcg cgaatccgcg ttcccggtat tccctccggg ggttaacccg gcgctggttg
     5221 cgaaccttat cgacatcatc ccgacggcaa cgatcaatcc cggcgaaaac atcacggcaa
     5281 cggcagcgat caattacgga atgggctcat tcgcgattgg catgaccgga tcggcatcct
     5341 gggaatcacc gacaatcatc acggatactc cgattttccg gcccggattg cgatatctgc
     5401 tcgcgctcca gtcatcgtac aaatggcccg aaaactgggg catcacggag ttgaccgcgg
     5461 cagtggcgca ttccaaccgc aacaaggtgc tgcggcgtga agcgacagaa ctggcattgg
     5521 aagatctcaa ttcgaactcc aatgtttacc gcatcgggct gcaacacctg tttccggtcg
     5581 cacaattcca ggtcgggccg accggcagcg tgctctatcg cgatcacaac ggctacaacg
     5641 caaccacatt gcaattcgtg ccgcagaaga agcggtgggc cgccggaatg gtcgcgcaat
     5701 acgtcccgaa tgaaaggatt acgatcaatg cacgcgtcga gcacgtctgg attcatg
//
LOCUS       DASSRL010000208         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1404983, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000208 DASSRL010000000
VERSION     DASSRL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404983"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..752
                     /locus_tag="VFL49_04310"
     CDS             99..752
                     /locus_tag="VFL49_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911228.1"
                     /translation="MRVSMLALAGILACAAPAALGQVVIDVESQRAAEPPQDQPQPQP
                     QPQPALPPQSAPDESHVQAKDDSRLAPAGGGRFTFNRIDNGFLRLDNQSGQVAYCRTQ
                     AAGWACEAVPENRAEPESDVARLQADLTALNALKSEVVRLQDEVASLKREIANLKEPP
                     PPPRPPADLTPPNKGGDATIKLPTQQDIARARDFIEKTWQRLVDMITAVQKDMMQKG"
     gene            788..1237
                     /locus_tag="VFL49_04315"
     CDS             788..1237
                     /locus_tag="VFL49_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7911229.1"
                     /translation="MSTIVEAAPQLIASATLHVDTPGTGFTDITENAASFVAQTGARE
                     GVLLVYLRHTSASLTIQENADPDVQRDLITALDRLAPEDAGWVHDVEGPDDMPAHVKA
                     MLNGVSVQVPVVAGNLALGTWQGIYLVEHRRRPHSREVVFQFIGSRT"
     gene            complement(1264..1632)
                     /locus_tag="VFL49_04320"
     CDS             complement(1264..1632)
                     /locus_tag="VFL49_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911230.1"
                     /translation="MEEMRRIAFETVQRACLFGSLAIFCVMVGMSFEPRLAFQAGGTL
                     TVLMALVLLYKAREALTKDYRRTEMWLYLPKNFRPPEAHAQWASSTVLRDTYLTFALW
                     TSAIAIAMWAIALLFTLAGV"
     gene            complement(1714..2136)
                     /locus_tag="VFL49_04325"
     CDS             complement(1714..2136)
                     /locus_tag="VFL49_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911231.1"
                     /translation="MAFAAEQSVIREAPMIPFTRFAFFTVARDAGFVTLAAATLMLAF
                     SFAPPLAFEIGATVALIFAIGLIFRAYLLTEVRFLQSEAWRALRADERPAGEHGLRLA
                     RAQFEELLLRAAKISSGIAGILYLTALVLSAGGAPIAL"
     gene            complement(2212..2538)
                     /locus_tag="VFL49_04330"
     CDS             complement(2212..2538)
                     /locus_tag="VFL49_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911232.1"
                     /translation="MSRNAFYIGIAFFGMINGIFNQVALLFTLIHMQILAPALLFGSP
                     GLTLMFSSLMVSTATIILAGVPAAIYERVVGAKDDSTAGSLWIWLAGTALLTLPAIGN
                     FFQYGL"
     gene            complement(2547..2882)
                     /locus_tag="VFL49_04335"
     CDS             complement(2547..2882)
                     /locus_tag="VFL49_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911233.1"
                     /translation="MPRISGDTQAVNSNSILSLWYFHLPNFVLAALMYTLLARALLGV
                     IVQMDSPNYIWRFFCRVTDPVIAVIALVTPKATVPVVLWLFSAVWMFWLRVGLLYLFL
                     LAGALPKKG"
     gene            complement(2961..>3499)
                     /locus_tag="VFL49_04340"
     CDS             complement(2961..>3499)
                     /locus_tag="VFL49_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911234.1"
                     /translation="PTIRRLTVARVLLVFVAIAAAATAASKPSDILAMVGWAFSLAMA
                     GNFPPLVLGIWWKRATTSGAICGMIAGFSLCLFYLVVTRYFPQAGVDYFGMTSMTNPV
                     TGANLVDITKPIAPSAAQRVGWFGLNNINCGLLGMPLGFLVIYVVSLFTKEPGREMQA
                     FIDEIRKPRGGTVLQEKT"
BASE COUNT          788 a         1064 c         1059 g          588 t
ORIGIN      
        1 ccgacggttc ttcgaggagc attggcgccc gggtttggcg tgcatagtgt cgatgctacg
       61 cttgtaaatt cccagcgtcg aaccgaagga acgagaacat gcgcgtctct atgctcgctt
      121 tggccgggat tctcgcctgt gctgcgccgg cggcgctggg gcaggtcgtc atcgatgtcg
      181 aaagccaacg tgccgctgaa ccgccgcagg atcagccgca gcctcagcct cagcctcagc
      241 ctgcacttcc gccgcagtca gcgcccgacg aaagtcacgt tcaggcgaaa gatgatagcc
      301 gcctggcgcc cgcgggcggt ggccgcttta cgttcaatcg tatcgacaac gggttcctcc
      361 ggcttgataa ccagagcggg caggttgcct attgccgcac gcaagctgcc ggctgggcat
      421 gcgaagcggt gccggaaaac cgtgccgaac cggaatcgga tgtcgctcgc ctgcaagccg
      481 accttacggc gctgaacgcg ctcaagtccg aggttgtgcg cttgcaagat gaagtcgcct
      541 cgctcaagcg cgagattgcg aacctgaaag agccgccgcc tccgccacga ccgccggcag
      601 atctgacgcc gccgaataaa ggcggagacg cgaccatcaa attgccgacg caacaggata
      661 tcgcgcgcgc tcgcgacttc atcgagaaga cgtggcagcg gctcgtcgac atgatcaccg
      721 ccgtgcaaaa ggacatgatg caaaagggct gagccgtccg acgccacgcg agtccatgcc
      781 acgccgaatg tccaccattg tcgaggccgc gccgcagctg atcgccagcg cgaccttgca
      841 tgtcgacacg ccaggcacag ggttcactga catcacagag aatgcggcga gtttcgtcgc
      901 ccagacaggc gcgagggagg gcgtgctgct ggtgtatctg cgtcatacat cggcttcgtt
      961 gacgattcag gagaacgccg atcccgacgt gcaaagagac ctgatcaccg cgctcgatcg
     1021 gctggcgcca gaggacgcgg ggtgggtcca cgatgtcgaa gggcccgacg atatgccggc
     1081 ccatgtcaag gcgatgctca atggcgtctc ggtgcaggtg ccggtcgttg cgggcaacct
     1141 cgcgctcggg acctggcagg gcatctacct tgtggagcac cggcgcaggc cgcacagccg
     1201 cgaagtcgtt ttccagttta tcggtagccg gacgtgacgc cgccgcgacg gtcctgcaga
     1261 tgatcaaaca cccgccaacg tgaacaaaag tgctatcgcc cacatggcga tcgcgatcgc
     1321 cgaagtccac agtgcaaaag taaggtaggt atcgcgcaag acggtcgagc tcgcccactg
     1381 ggcatgggct tccggcgggc gaaaattctt cggcagataa agccacatct ccgttctgcg
     1441 gtagtctttg gtgagcgctt ctcgtgcctt gtaaaggaga accagcgcca tcagtacggt
     1501 cagcgtgccg cctgcctgaa aggccagccg cggctcaaac gacatgccga ccatgacgca
     1561 aaaaatagcg agcgaaccga aaagacacgc gcgctgcacg gtctcgaatg caatccgacg
     1621 catctcttcc atgcatggcc cccgagtcca tcgttccggc cggattcaac ggatactagc
     1681 ggcaaatccg tgacgcaagc attcacgaag gcgttaaagc gcgattggcg cgccgccagc
     1741 cgacagaaca agggcagtca ggtagagaat tccggcaatt cccgaggaaa tcttggcggc
     1801 ccgcagcagc agctcctcaa attgcgcgcg tgccaagcgc agtccgtgtt caccggccgg
     1861 acgctcgtcc gcccgcagcg cccgccacgc ttcgctctgc aggaatcgga cctccgtcaa
     1921 cagatacgcc cggaagatga gaccgattgc gaaaatcagc gccactgttg caccgatctc
     1981 gaaggccagt ggaggtgcaa aactaaatgc cagcatcagt gtcgcggcgg cgagcgttac
     2041 aaacccggca tcgcgggcta cggtaaaaaa tgcaaatcgc gtgaaaggga tcatcggggc
     2101 ttctcggatc accgactgct cggcagcaaa tgccatgcca atccgacgga tttcacagca
     2161 cgttactgca aacaccgcaa attgcgatgg gttccctgtg caggcaaagt gttataggcc
     2221 gtattgaaag aaattgccga tggcgggcag cgtgagcagt gccgtgcctg caagccagat
     2281 ccataacgaa ccggcagtcg aatcatcttt cgcgcccacg acccgctcgt aaatcgcggc
     2341 cggaacgccg gcgaggataa tcgtggcggt cgaaaccatc aacgaagaga acatcagcgt
     2401 caggccgggg ctgccaaaca gcagcgcggg cgcaagaatt tgcatgtgga tcaacgtaaa
     2461 gagcagtgca acctgattga agatgccgtt gatcatcccg aagaaggcta taccgatgta
     2521 gaaggcgttg cggctcattg cgcgtgtcaa ccttttttcg gcagggcgcc ggcaagcaga
     2581 aacagataga gcaacccgac ccgcagccag aacatccata ccgcgctgaa cagccaaagc
     2641 acgacgggta ccgtcgcctt cggcgtcacc aacgcgataa cggcaatgac gggatcggtg
     2701 acccggcaga aaaaccgcca gatgtagttc ggcgaatcca tctgcacgat cactccgaga
     2761 agtgcgcgtg cgagcagggt gtacatcaac gccgccagaa cgaagtttgg caggtgaaaa
     2821 taccagaggc tcaggatcga gttgctgttc acggcctggg tgtctccgga aatgcgcggc
     2881 atgttagccg attgccagcg ggtgccgcac aaaaaagcgg ggccccgaaa gggccccgct
     2941 cccgtcaatc ggatttgtga tcaagtcttt tcttggagca ccgttccgcc acgcggtttg
     3001 cggatctcgt cgatgaaggc ttgcatttcc ctcccgggct ctttggtgaa taggctgacc
     3061 acgtaaatca caaggaagcc gagcggcatt ccaagcaatc cacagttgat gttgttcaac
     3121 ccgaaccagc caacccgttg cgcggcgctc ggcgcaatcg gcttggtgat gtcgaccagg
     3181 ttggcgcctg tgaccgggtt ggtcatggac gtcatgccga aatagtcgac gcctgcctgc
     3241 gggaaatatc tcgtcaccac gagatagaac aggcagaggc tgaagcccgc gatcatgccg
     3301 caaatggcgc cgctggtcgt cgcgcgcttc caccagatgc cgagcacgag cggcgggaag
     3361 ttacccgcca tcgcgagcga gaacgcccag cccaccatgg caaggatatc cgacggtttc
     3421 gaagccgcgg ttgccgctgc ggcgatggcc acgaacacca gcaacacgcg ggcaacggtc
     3481 agccgccgga tcgtcggcg
//
LOCUS       DASSRL010000209         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_373084, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000209 DASSRL010000000
VERSION     DASSRL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373084"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFL49_04345"
     CDS             <1..988
                     /locus_tag="VFL49_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7911235.1"
                     /translation="LGDLDLLVGTHALFQEDVAFHDLALAVIDEQHRFGVHQRLALTQ
                     KGEAVDVLVLTATPIPRTLVLTYFGDMDISELREKPPGRQPIDTRAIPLSRLEEVEDA
                     VGRALAEGKRAYWVCPLVDESEKIDLAAAQARFEELRKKFGDQVDLVHGRMKGADKDA
                     AMARFADGDTRLLVATTVIEVGVDVPEATIMVIEHAERFGLAQLHQLRGRIGRGSGAS
                     TCLLLYKAPLGATAKARLAILRETEDGFRIAEEDLRLRGEGDLLGTRQSGWPGFRIAL
                     PELHGKYLGAARDDAALILSRDPTLSHPRGEALRHLLYIFGKDEAVKLIRAG"
     gene            complement(991..1752)
                     /locus_tag="VFL49_04350"
     CDS             complement(991..1752)
                     /locus_tag="VFL49_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET7911236.1"
                     /translation="MTEPAGNSSDPLAVDVAKTTSSHTSLAGRLRNYFLTGLVVAGPL
                     AITIWLIWSLITWVDGFMRPVIPPDYRPESYLPWPIPGTGLIIAVVGLTLLGFLTANL
                     VGRTLVGWGEGVLDRLPIVRPIYKTMKHVFEALFSKTGSSFRKVALVEFPAPGMWSLV
                     FLSQPPSGDLANKLPSGEHVSAFMPCTPNPTTGFFFYVKRKDLIELDISVENAMTLLI
                     SAGMVQPEGEVQKNLAALAVKAKAARAKRKAEAAE"
     gene            complement(1763..3589)
                     /gene="glmS"
                     /locus_tag="VFL49_04355"
     CDS             complement(1763..3589)
                     /gene="glmS"
                     /locus_tag="VFL49_04355"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7911237.1"
                     /translation="MCGIVGILGNKPVAGQLVDALKRLEYRGYDSSGVATLEKGVLGR
                     RRAEGKLRALENRLEKEPLAGTIGIGHTRWATHGRPTEDNAHPHATDLVAVVHNGIIE
                     NFRELREKLSKNGSKFASDTDTEAVAHLVTQEMKSGKSPEDAVAASLKQLRGAFALAF
                     LFTGKDDLLIGARKGSPLAVGYGNGEMYLGSDAIALAPFTDTISYLEDGDWAVLTRKG
                     AQIRDEQDREVERPIVKSTASAQLVDKGNHKHFMAKEIHEQPEVVGHTLAHYIDMINE
                     RVALPGALPFDWTKLKRVSISACGTAFYAGLVAKYWFERFAKLPIEIDIASEFRYRGA
                     PLEPGDLAVFVSQSGETADTLATLRYAKENKQHILSVVNVPTSTIARESDVIMPTLAG
                     PEIGVASTKAFTCQLSALACLAIAAGRARGVLSPADEQKLVHALVETPRLMAAALALE
                     PQIERLAQNLAKARDVLYLGRGTSFPIALEGALKLKEISYIHAEGYAAGELKHGPIAL
                     IDETMPVIVIAPYDGVFDKTVSNMQEVAARGGRIILVTDPRGAKEAAIASVETITLPD
                     MSTTITPLVYAIPVQLIAYHTAAAIGTDVDQPRNLAKSVTVE"
     gene            complement(3682..>4775)
                     /gene="glmU"
                     /locus_tag="VFL49_04360"
     CDS             complement(3682..>4775)
                     /gene="glmU"
                     /locus_tag="VFL49_04360"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7911238.1"
                     /translation="ARGADDILVMFSDTPLVRPETLLKLRDALAAGAAVAVLGFRPAN
                     PQGYGRLLMQDRELVAIREEQDANLEERKIGFCNGGLMALAGDRALGILDRIGNSNAK
                     GEYYLTDAVAVARDMGLKTTAIETSEDDVRGINTKTQLAETEAVLQQRLRAAAMEAGV
                     TLVAPETVFLSADTKFGKDVTVEPNVVFGPGVVVEDSATIRSFSHLEGARVGKGARVG
                     PFARLRPGADLGKDVHIGNFVEVKEATIEAGAKANHLTYIGDARVGEGANVGAGTITC
                     NYDGVAKHRTDIGKGAFIGSNTALVAPVKVGDGAYIGTGTVVTKDVPAGALAIARAKQ
                     TVKEGWAKRLRQVKALGKNLARKKAKRPS"
BASE COUNT          877 a         1544 c         1425 g          929 t
ORIGIN      
        1 gctcggggat ctcgatcttc tcgtcgggac acatgcattg ttccaggaag atgtcgcatt
       61 tcatgacctt gcgcttgccg tgatcgacga gcagcatcgc ttcggggttc atcaacggct
      121 ggccttgacg caaaaaggcg aagccgttga cgtgctggta ctcacagcga cgccgatccc
      181 gcgcacactc gtcctcacct atttcggcga catggacatt tccgaattgc gcgagaagcc
      241 ccctgggcgg caaccgatcg atacgcgcgc catcccgctc tcccgccttg aagaagtcga
      301 agacgcagtc ggccgcgcgc tcgcagaggg caagcgcgcc tattgggtct gtccgctggt
      361 cgacgagtcc gaaaaaatcg acctggccgc ggcgcaagcc cgtttcgagg agctacgcaa
      421 gaagttcggc gatcaggtcg acctcgtgca cgggcggatg aagggtgccg acaaggatgc
      481 cgcgatggcc aggtttgccg atggcgacac gcggctgctt gtcgcaacga ccgtcatcga
      541 ggtcggcgtc gacgtgccgg aggcaacgat catggtgatc gagcacgccg agcgcttcgg
      601 gctcgcgcaa ctccatcagt tgcgcggacg gatcgggcgc ggatcgggtg catcaacctg
      661 cctgctgctc tacaaggcgc cgctcggcgc gactgcaaag gcacggcttg cgattttgcg
      721 tgaaaccgaa gacggctttc ggatcgccga ggaagacctg cgcctgcgcg gcgaaggcga
      781 tctccttggc acgcgccaaa gcggctggcc cggtttccgg attgcgctgc ccgaactgca
      841 cggaaaatat ctcggcgccg cacgcgacga cgcggccctc atcctttcgc gtgatccgac
      901 gctatcgcac ccgcgcggtg aggccctgcg tcacctgctc tacattttcg gaaaagacga
      961 agcggtcaaa ttgataagag ccggctgagc ctattcggcc gcctcggcct tgcgcttggc
     1021 gcgcgcagcc tttgccttga cagcaagcgc ggccagattt ttctgcacct cgccctcggg
     1081 ctgcaccatg ccggcggaaa tcagaagcgt catcgcgttt tcgaccgaaa tgtcgagttc
     1141 gatgagatcc ttgcgtttta catagaagaa aaatccggtg gtcggattgg gcgtgcaggg
     1201 catgaacgcc gacacatgct cgccggacgg cagcttgttg gccaggtcgc cgctcggcgg
     1261 ctgcgacaga aacaccagcg accacatgcc gggcgccgga aactccacca gcgccacctt
     1321 cctgaaactg gaaccggttt tcgaaaacaa cgcctcgaaa acatgcttca tcgtcttgta
     1381 gatcggccgc acgatcggca ggcggtcgag cacgccctcg ccccagccga ccaaggtccg
     1441 gccgacgaga tttgctgtga ggaacccgag cagcgtcagt ccgaccaccg cgataatcag
     1501 tcccgtcccc ggaatcggcc acggcaggta gctctccggc cggtaatccg gcggaatgac
     1561 cggccgcatg aaaccgtcga cccaggtgat aagcgaccag atcagccaga tggttatggc
     1621 aagcgggcct gcgaccacga gccccgtcag gaaatagttg cgcagccggc cggcgaggct
     1681 cgtgtgcgag gacgtagttt ttgcgacgtc cacggctaat ggatcgctgg aattgcccgc
     1741 cggttctgtc atgagggcca gtctattcta ccgtaacgga tttcgcgagg ttccgtggct
     1801 ggtccacatc cgtgccgatt gcggcggcgg tatgatacgc aatcaattga accggaatcg
     1861 catagaccaa tggcgttatt gtcgtgctca tgtccggcaa agtgatggtt tcgaccgatg
     1921 ctattgccgc ctctttcgcg ccgcgcggat ccgtcaccag gatgatcctg ccaccgcgag
     1981 cggcgacctc ctgcatgttc gacacggttt tgtcgaacac gccgtcgtac ggcgcgatca
     2041 cgatcaccgg catcgtctcg tcgatcagag caatcggccc gtgcttgagt tcgcccgcgg
     2101 cgtagccctc ggcgtgaatg taggaaattt ccttgagctt gagcgcgccc tcaagcgcga
     2161 tcggaaagct ggtgccgcgg ccgaggtaaa gcacgtcgcg cgccttcgcg agattctgcg
     2221 cgagtctttc gatttgcggc tcgagcgcga gcgcagccgc cataagtcgc ggtgtctcga
     2281 cgagggcatg caccagcttc tgttcatccg ccggcgacaa cacgccacgc gcgcgcccgg
     2341 ctgcgatcgc caggcaggca agcgcgctca gctgacaggt gaaggccttg gttgaggcca
     2401 caccgatttc cggtcccgcc agcgtgggca tgatgacatc gctctcgcgc gcgatcgtcg
     2461 aggtcggcac gttgaccacg gagagaatgt gctgcttgtt ctctttcgcg tagcgcagcg
     2521 ttgccagcgt gtcggcggtc tcgcccgact gcgaaacgaa gaccgcaagg tcgcccggct
     2581 ccagcggagc gccgcgataa cgaaactcgg aggcaatatc gatctcgatc ggcagcttgg
     2641 caaagcgctc gaaccagtat ttcgccacca accctgcata gaatgccgtg ccgcaagccg
     2701 aaatcgaaac cctcttcagc ttcgtccagt cgaacgggag cgcacctggc agcgcgaccc
     2761 gctcgttgat catgtcgatg tagtgcgcaa gcgtatgccc gaccacttcc ggctgctcgt
     2821 ggatctcctt cgccatgaag tgcttgtggt tgcccttatc gacgagttgg gccgacgccg
     2881 tggatttaac aatcggccgt tcaacctcgc ggtcctgttc gtcgcgaatt tgcgcgccct
     2941 tgcgcgtgag caccgcccag tcgccgtctt caaggtagct gatcgtgtcg gtgaacggcg
     3001 caagcgcgat cgcatcggac ccgagataca tttcgccgtt gccgtagccg acggcgagcg
     3061 gcgacccctt gcgggccccg atcaggaggt cgtctttgcc ggtaaaaaga aacgccagcg
     3121 caaaggcgcc gcgcaactgc ttgagcgagg ccgcaaccgc atcctcgggg gacttgccgc
     3181 ttttcatctc ctgcgtgacc agatgcgcca ccgcctcggt atcggtgtca ctggcaaatt
     3241 tgcttccgtt tttgctcagc ttttcacgca gctcgcggaa attttcgatg atgccgttgt
     3301 gcacgaccgc aaccagatca gtcgcgtgcg gatgggcatt gtcttcggtc ggccggccat
     3361 gagtcgccca gcgggtgtga ccgatgccga tcgtgccggc gagcggctct ttctcaagcc
     3421 ggttctcgag cgcgcgcaat ttgccctcgg cgcgcctgcg gccaagaacg cccttctcaa
     3481 gagtggcgac gccggacgag tcgtagccgc ggtactcaag gcgcttgagc gcgtcgacca
     3541 actggcccgc gaccggcttg tttccgagaa ttccgacgat tccgcacatc cgctgggaac
     3601 ggccccttca cccaccgcta tggtttagcc accatcgaca aacgcacgga aatcaaaacg
     3661 catttcgccg tgtgacagcc ctcatgacgg ccgttttgcc ttctttctcg ccaggttttt
     3721 gcccaaggcc ttcacctgcc gcaagcgttt tgcccagcct tccttaaccg tctgcttggc
     3781 gcgcgcgatc gccagtgcgc ctgccggaac gtccttcgtg acaactgttc ccgtgccgat
     3841 ataggcccca tcgccgactt tgaccggcgc aacaagtgcg gtgttggaac cgataaacgc
     3901 gccttttccg atatccgtgc gatgctttgc cacgccgtca tagttgcagg tgatggtccc
     3961 tgctccgaca ttcgcgccct cacccacgcg tgcatcgccg atatatgtca ggtggttggc
     4021 cttcgcgccg gcctcgatcg tggcttcctt gacctcgacg aaattaccga tgtgcacatc
     4081 cttaccaagg tcggctcccg gccgcaggcg cgcaaacggg ccgacgcgcg cgcccttgcc
     4141 gacgcgcgca ccttccagat gcgaaaacga tcggatcgtc gcgctgtctt ccacaacaac
     4201 gcccggcccg aacacaacat tcggctcgac ggtgacgtct ttgccgaatt tggtgtcagc
     4261 cgacaaaaat accgtttccg gggcgaccaa ggtgacgccc gcttccatcg cggccgcgcg
     4321 cagccgctgc tgcagcacag cctcggtctc ggcgagttgc gtcttcgtat tgattccgcg
     4381 cacgtcgtcc tccgaggttt cgatcgccgt tgtcttcaag cccatatcac gggcaaccgc
     4441 aacggcatcg gtgagataat actcgccctt tgcgttgctg ttgccgatac ggtccaggat
     4501 gccaagcgcg cgatcgccgg cgagcgccat cagtccgcca ttgcagaagc cgattttgcg
     4561 ctcttccaga ttcgcgtcct gttcctctcg gatcgcaaca agctcgcggt cttgcatcaa
     4621 aagccggccg tacccctgcg ggttcgccgg cctgaagccg aggaccgcca cggcggcgcc
     4681 ggctgcgagc gcgtcgcgca gctttagaag cgtttcgggg cgaaccaaag gcgtgtcgct
     4741 gaacatgacg agaatatcgt cggcgccacg cgcaa
//
LOCUS       DASSRL010000210         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1055450, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000210 DASSRL010000000
VERSION     DASSRL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055450"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFL49_04365"
     CDS             complement(<1..703)
                     /locus_tag="VFL49_04365"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET7911239.1"
                     /translation="MAVVLDLVNNPRPRHPEKAHRPDSPVLKKPDWIRVKAPVSPGYF
                     TTQKIMRDNGLHTVCEEAGCPNIGECWEKKHATFMIMGDTCTRACAFCNVKTGLPAAL
                     DPSEPTQVADATAKLGLAHIVVTSVDRDDLPDGGARHFAETIRAIRARCLQTTIEVLT
                     PDFLRKDGALEIVVEAKPDVFNHNLETVPSLYLTVRPGARYFHSLRLLQRVKELDPAI
                     FTKSGIMVGLGEDRNE"
     gene            complement(788..>2059)
                     /gene="lpdA"
                     /locus_tag="VFL49_04370"
     CDS             complement(788..>2059)
                     /gene="lpdA"
                     /locus_tag="VFL49_04370"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7911240.1"
                     /translation="PTKALLRSAEVFHYFQHAKDYGLKADNVGYDPKAVVARSRAVSK
                     RLNDGVGFLMKKNKVSIIWGEASIDAPGKVTIKTSKSEAPKGALGAGNYQAKHIIIAT
                     GARPRVLPGIEPDKKLVWTYFEAMVPDRVPKSLLVIGSGAIGIEFASFYRTLGSDVTV
                     VEVLPQILPVEDQEIQAFARKSFEKQGIKIHTGAKVTKLEKKSDSVTATIEEGGKSQT
                     LTVERVISAVGVVGNIENLGLEKLGVKTDRGTIVVDKYNRTNVPGICAIGDVAGPPML
                     AHKAEHEGVICIEAIKGLNPHPMNKLMIPGCTYCSPQVASVGLTEQAAKEKKLDIRVG
                     RFPFMGNGKAIALGEDQGLVKVIFDRKTGQLLGAHMVGAEVTELIQGYVVAMNLETTE
                     EELMHTVFPHPTLSEMMKEAVLDAYGRVLNM"
BASE COUNT          414 a          646 c          547 g          452 t
ORIGIN      
        1 cttcattccg gtcttcgccg agaccaacca taattcccga cttcgtgaaa atagccggat
       61 caagctcctt cactcgctgc aacagccgca acgaatgaaa gtagcgcgcg cccggacgca
      121 cggtgagata aagcgacggt actgtctcaa gattgtgatt aaagacgtcc ggctttgcct
      181 cgacgacaat ttccaacgcg ccgtccttgc ggagaaaatc cggtgtcagc acctcaatcg
      241 ttgtttgcag acagcgcgcg cggatggccc ggatcgtctc ggcaaaatgc cgcgcaccac
      301 catccggcag gtcgtcgcga tcgacggacg tcaccacgat gtgagcgagg ccaagtttcg
      361 ccgttgcatc ggctacctgc gtcggctcgc tcggatcaag cgccgcgggc agcccggtct
      421 tcacattgca aaaggcgcag gcgcgggtgc acgtgtcacc catgatcatg aaggtcgcgt
      481 gcttcttttc ccagcattcg ccgatattcg ggcagccagc ctcctcgcac accgtgtgca
      541 ggccattgtc ccgcatgatc ttctgcgtgg tgaaatagcc gggtgagacc ggtgccttga
      601 cgcgaatcca gtcaggcttt ttcaggacgg gcgaatccgg ccggtgcgcc ttttccggat
      661 ggcgcggacg cgggttgttc accagatcga gaacgactgc catggcccct acttacggcc
      721 gcgccctgtg cgccgcaagg ccgccccgac gccgacaatc gtgccgcaga tacagcagat
      781 aaacccgcta catgttcagc acgcgcccat aggcatcgag cacggcttct ttcatcattt
      841 ccgacaaggt cgggtgcggg aacaccgtat gcatcagctc ttcctccgtc gtctcaaggt
      901 tcatggcaac gacgtatccc tggatgagct cggtcacttc ggctccaacc atatgcgcgc
      961 cgagcagctg accggtcttc cggtcgaaaa tgaccttcac cagaccctga tcctcgccga
     1021 gcgcaattgc cttgccgttg cccatgaaag gaaatcggcc gacacgaata tcaagtttct
     1081 tttccttcgc cgcctgctcg gtcaggccca cggacgcgac ctgcggcgag caataggtac
     1141 agccagggat catcaacttg ttcattggat gcgggttgag acccttgatt gcctcaatgc
     1201 agataacccc ttcgtgctcg gccttgtgcg caagcatggg agggccggct acgtccccga
     1261 tcgcgcagat gccggggacg ttcgtcctgt tgtatttgtc gacgacaatc gtacctcgat
     1321 ctgtttttac gccaagcttt tcaaggccca aattctcgat gttgccgaca acaccgacgg
     1381 ccgagatcac gcgctcaaca gtcaacgtct gcgatttgcc gccctcttct attgtcgctg
     1441 tgacgctgtc gctcttcttt tcaagcttgg tgaccttagc gccggtatga atttttattc
     1501 cttgcttctc gaagcttttg cgggcgaatg cctgaatctc ctgatcttca accgggagaa
     1561 tttgcggcag cacctccacc accgtcacgt ccgagccgag cgttcgatag aacgaagcaa
     1621 actcgatccc gattgcgccc gatccgatga ccagaagcga cttcggtacg cgatccggca
     1681 ccatcgcctc gaaataagtc cagaccaatt tcttgtcggg ctcgatgccc ggcaacacgc
     1741 gcggtcgggc gccggtcgca atgatgatat gtttggcctg ataattgccg gcaccaagcg
     1801 cacccttcgg cgcttcgctc ttagaagtct ttatggtcac tttgccgggt gcatcgatcg
     1861 aggcctcgcc ccaaatgatc gagaccttgt tcttcttcat cagaaagcca acaccgtcgt
     1921 tgagccgttt cgataccgcg cgtgagcgcg caacgaccgc tttcggatcg tacccgacat
     1981 tgtctgcctt caaaccgtaa tccttggcgt gctggaaata gtgaaagacc tccgccgaac
     2041 gcagcaaagc cttggtcgg
//
LOCUS       DASSRL010000211         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1597649, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000211 DASSRL010000000
VERSION     DASSRL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597649"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFL49_04375"
     CDS             <1..350
                     /locus_tag="VFL49_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET7911241.1"
                     /translation="SGLRARISDTVALPVPSAREIRYRAVVGKEMVLGLRPEHITEPR
                     HNGRADGRDFEVTLDVVEPMGMETMVFFSINGTEICSRVEPSSAGDAGSKMRLYANVD
                     HMHLIDPATGAVI"
     gene            complement(358..1458)
                     /locus_tag="VFL49_04380"
     CDS             complement(358..1458)
                     /locus_tag="VFL49_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HET7911242.1"
                     /translation="MSSSLFTPFRLAGLELANRIVVSPMCQYSADDGCANDWHLMHLG
                     MMAHSGAALVVVEATHVERHGRITHGCLGLYSDDNEAALARVIAQARRMGGAKFGIQI
                     AHSGRKGSAQRPWEGAGPLQAEADPWPTIAASPIPFGDGWQTPREVTEADMERVRAAF
                     VSAAQRALRIGFDEIELHMAHGYLAHGFMSPISNKRTDQYGGSLENRLRFPLSIARAV
                     RAVVPKSVVLGARITGSDWREGGLTPDDAVVISRALKGEGVDFICISSGGVAADIRNP
                     TEPGYNVPIAARVKREANIPTRTVGLIVKPEHAEEVVAKGEADMVSMARCFLDDPHWG
                     WHAAKALGADVARPPQYQRAGPKLWAPAAAKA"
     gene            complement(1534..1911)
                     /locus_tag="VFL49_04385"
     CDS             complement(1534..1911)
                     /locus_tag="VFL49_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911243.1"
                     /translation="MQLPRIVVHGGFTLYAVVFLKLSVVVDALRVVRLPAFTRAGGHS
                     MAVVQGGVRFDRIAQKWHALAQRRLAYVRELERSGRWKHFYSEEQFRLYLRETERVAA
                     LWAKLAAGRFAVATGTNSMIPAA"
     gene            complement(1927..>2686)
                     /locus_tag="VFL49_04390"
     CDS             complement(1927..>2686)
                     /locus_tag="VFL49_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911244.1"
                     /translation="ALYFGWASYLSAYSAKAWGFPPELAIVTGTATAAVCGLVAGSLA
                     IRRQGIYFAMITLALAQMMYFFALQAKFTGGEDGIQAVPRGHLFGIFDLKNVMTMYVV
                     VLIIFILGFSFIYRIIHSPFGEVLKAIRENETRAISLGYKTDRYKLVAFILSATLAGV
                     AGATKAIVLQLASLTDVNWPMSGEVVLMTLVGGLGTIFGPVVGAFVILVMQFKLAPIG
                     EWVLVIQGAIFVACVLLFRRGIVGEIANRLRIRL"
BASE COUNT          560 a          868 c          809 g          449 t
ORIGIN      
        1 gctctggtct gcgtgcgcgt atctccgaca cggttgcatt gcccgttcca tcagcccgcg
       61 agatccgcta tcgagccgtt gtcggcaagg aaatggtcct tggtctgcgg ccggagcaca
      121 tcaccgagcc gcgccataac ggccgtgccg atggccgcga tttcgaagtg accctcgacg
      181 tcgttgagcc gatgggcatg gagacgatgg tcttcttttc catcaacggc acagagattt
      241 gcagccgggt cgaaccgagc agtgcgggcg acgcaggatc gaaaatgcgc ctgtacgcca
      301 acgtcgatca catgcacctg atcgatcctg cgaccggcgc agtcatctga cgacggttca
      361 ggcttttgct gcggccggcg cccaaagctt cggtccggcc cgctgatact gtggcggccg
      421 tgccacatcc gcgccaagcg ccttcgccgc atgccagccc caatgcggat cgtcgaggaa
      481 gcagcgcgcc atcgaaacca tatcggcctc gcctttggcg acgacctctt cggcgtgctc
      541 gggcttgacg atcaggccga cggtacgggt cggaatattc gcctcgcgct tcacccgcgc
      601 cgcgatcgga acgttgtagc ccggctcggt ggggttgcgg atgtcggcgg caactccacc
      661 ggacgagatg cagatgaaat cgacgccctc gcctttcagc gcccgcgaga ttacgacggc
      721 gtcgtcgggc gtcagtccgc cctcgcgcca gtcgctgccg gtaatacgcg caccgagcac
      781 gaccgatttc ggcaccaccg cccgcaccgc gcgcgcgatc gatagcggaa aacgcaggcg
      841 gttttccagt gagccgccat attgatcggt gcgcttgttc gaaatcggcg acatgaagcc
      901 atgtgccagg taaccatgcg ccatatgcag ctcgatctca tcgaatccga ttcgcagcgc
      961 gcgttgcgcg gctgaaacaa aggctgcgcg cacgcgctcc atgtcggcct cggtgacttc
     1021 gcgcggcgtc tgccagccat cgccaaaggg aatcggcgag gctgcaatcg tcggccatgg
     1081 atccgcttcc gcctgcagcg gccccgcgcc ctcccatgga cgctgcgccg aacctttcct
     1141 gccggagtgc gcaatctgga tgccgaattt tgccccgccc atgcggcggg cctgcgcgat
     1201 cacacgcgcc agcgcggcct cattgtcgtc ggaataaagc ccgaggcaac catgtgtgat
     1261 gcggccgtgc cgttcgacgt gcgttgcttc cacaaccacc aaggcggcgc cggaatgcgc
     1321 catcatgccg agatgcatga gatgccagtc attggcgcag ccatcgtcgg ccgagtactg
     1381 gcacatgggc gagacgacga tgcggttggc aagctccaga ccggcaagac gaaagggtgt
     1441 gaacaagcta ctggacatga tgttgattga cactcttggt gggaaagctg ctggctctcc
     1501 agatagccga tgatggcacc ggcgtcacgc gtctcaagcg gccggaatca ttgagttcgt
     1561 gcccgtcgcc actgcaaatc ggcctgccgc cagcttggcc caaagggccg caacacgctc
     1621 cgtttcgcgc aggtacaagc ggaattgctc ttcggaataa aaatgcttcc aacggccgct
     1681 gcgctcaagc tcgcggacgt aggcaagccg ccgctgcgcg agcgcatgcc atttctgcgc
     1741 aatcctgtcg aagcggactc cgccctgcac aaccgccatt gagtgccccc cggcacgcgt
     1801 gaaagccggc agccgaacga cgcgcaacgc atcgacgacc accgaaagct tcaggaaaac
     1861 aacggcatac agagtgaaac cgccatgtac gacgattcgc ggcagttgca aagaggtaac
     1921 tggaaactac aggcggatgc gcagcctgtt ggcgatctcg ccgacgatgc cgcggcggaa
     1981 aagaagcacg caggcaacga aaatcgcgcc ctggatgacg agaacccact cgccgatcgg
     2041 cgcaagcttg aactgcatca ccaggatcac aaaggctcct accaccggcc cgaagatcgt
     2101 tcccaggccg ccgacaagcg tcatcagtac cacctcgccc gacatcggcc agttcacgtc
     2161 ggtgagcgac gccagttgca gcacaatggc cttggtcgcg ccggcgacac cggccagcgt
     2221 cgccgacagg atgaacgcga ccagcttgta gcggtcggtt ttgtacccaa gcgagatcgc
     2281 gcgcgtctcg ttctcccgga tggccttgag cacttcgccg aacggcgaat ggatgatccg
     2341 gtagatgaaa gaaaagccga gaatgaagat gatcagcacg accacataca tggtcatgac
     2401 attcttgaga tcgaaaatac cgaagagatg gccgcgcggc acagcctgaa tgccgtcttc
     2461 accaccggtg aacttcgcct gcagcgcgaa gaaatacatc atctgcgcaa gcgcgagcgt
     2521 aatcatggcg aagtagatgc cctggcggcg gatcgccagc gacccggcaa ccagtccgca
     2581 caccgcagcg gtcgcggtgc cggtcacgat cgcaagctcg ggcgggaaac cccacgcttt
     2641 ggcggaatag gccgatagat agctcgccca gccgaaatag agcgca
//
LOCUS       DASSRL010000212         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1527834, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000212 DASSRL010000000
VERSION     DASSRL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527834"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="tsaD"
                     /locus_tag="VFL49_04395"
     CDS             complement(<1..882)
                     /gene="tsaD"
                     /locus_tag="VFL49_04395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7911245.1"
                     /translation="MIVLGIETTCDETAAAVVERFEDGRGKILSNVVLSQVREHAAFG
                     GVVPEIAARAHVETLDGIIARAMAQADRGYDAIDGIAAAGGPGLIGGVIVGLTTAKAI
                     ALVKNKPLIAVNHLEAHALTARLTDNTPFPYCLFLASGGHTQILAVRGVGDYLRLGTT
                     QDDAIGEAFDKTAKLLGLGFPGGPEVEREAMRGSPTRFDLPRPMHARKEPDFSLSGLK
                     TALRLEAEKIAPLSDQDVADLCAGFQQAVVDVVFDRLRAGLRLFRARVGTPTALVAAG
                     GVAANQSIRRALNRLAFE"
     gene            995..1921
                     /gene="hemC"
                     /locus_tag="VFL49_04400"
     CDS             995..1921
                     /gene="hemC"
                     /locus_tag="VFL49_04400"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET7911246.1"
                     /translation="MAQSKASLRIGTRGSPLALVQARLVRSRLSAAAGLSEDAIELVV
                     IRTTGDTIQDRPLAEEGGKGLFTKEIEEALLDGRVDLAVHSAKDMPTLLPTGLILAAC
                     LEREDPRDVFISRQARSLAELPRGASLGTASLRRQAIAKRARPDLRILPLRGNVETRL
                     RKLDAGEADATILALAGLKRLGLADQAAAVLGADEFLPAVGQGAIGIEAREDNAGVRD
                     ILACIDHGETSTAVACERAFLGALDGSCKTPIAGHATVSGNAVQFRGLIARPDGSAAY
                     DIAGTGKRKDAATIGADAGRELKHRAGPGFFD"
     gene            1925..>2605
                     /locus_tag="VFL49_04405"
     CDS             1925..>2605
                     /locus_tag="VFL49_04405"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET7911247.1"
                     /translation="MRIVVTRPQSDGERTAAALEALGHEVLVAPLMRIEPVAADLDGA
                     WSAIAVTSANAPWTVTGTRLKTLPMFAVGQRSAEAARRAGFGDVTSANGDIMALVRLI
                     AARAAGAKVPLLYLAGEDRSGDLVTQLAAHGVEAEMRIVYRTVAEVFPPVLAAALEAG
                     DVDAVLHFSRRSAELFVEGAKASGVAGSAEDVRHFCLSAQIAEPLAGASSVAIAPRAD
                     EAALIALLP"
BASE COUNT          430 a          832 c          888 g          455 t
ORIGIN      
        1 ctcgaatgcg agacggttga gagcgcggcg gatggactgg ttggcggcga cgccgcctgc
       61 ggcaaccagc gccgtgggcg tgccgacgcg cgcgcggaac agccgcaagc cggcgcgcag
      121 acgatcgaac acgacgtcga ccaccgcttg ctgaaagccg gcgcaaaggt cggcgacgtc
      181 ctgatcgctg agcggagcga tcttttcagc ctccagccgc aacgcggtct tgaggccgga
      241 caatgaaaaa tccggctctt tgcgggcgtg catcggccgc ggcaggtcga agcgtgtcgg
      301 gctgccgcgc atcgcttctc tttcgacctc cgggccgccc ggaaagccaa ggccaagcaa
      361 tttcgcggtc ttgtcgaagg cttcgccgat ggcgtcgtcc tgcgttgtgc caaggcgcaa
      421 atagtctccg acgccgcgca cggcgaggat ctgcgtatga ccgccagagg cgaggaacag
      481 gcaatacgga aacggcgtat tgtcggtcag acgcgcggtc agcgcgtggg cttcaaggtg
      541 attgacggcg atcagaggct tgttcttcac cagagcaatc gccttcgccg ttgtcaggcc
      601 gacgatgacc ccgccgatca atccagggcc gcccgcggcc gcgattccgt cgatcgcgtc
      661 gtagccgcgg tcggcctgcg ccatggcccg ggcgatgatg ccgtccagcg tctccacgtg
      721 ggcgcgtgca gcgatctccg gcaccacgcc gccgaatgcg gcatgctcgc gcacctgcga
      781 gaggaccacg ttggataaaa tcttgccgcg gccgtcctcg aaacgctcga cgaccgcggc
      841 ggcggtttcg tcgcaagtcg tttcaattcc gaggacgatc atgattcctc atctgaatcc
      901 gccaccctag gcaggcgagg tgacggcagc ttaattaata gctagttctg gcgacagggc
      961 cgaccccggt gccggcctag cattgcgagg ttacatggcg caatccaaag cgtcgttgcg
     1021 catcggtacg cgcggcagcc cgctcgccct ggtgcaggca cgcctggtgc gctcgcgcct
     1081 ctcggcggcc gccgggcttt ccgaagacgc gatcgagctc gtcgtcattc ggacgaccgg
     1141 cgacaccatc caggaccggc cgttggcgga ggagggcggc aaaggattgt tcaccaagga
     1201 gatcgaggag gcgttgcttg atgggcgcgt ggacctcgcc gtccactcag cgaaggacat
     1261 gccaactttg ttgcccactg ggcttatcct ggctgcgtgc cttgagcgtg aagatccacg
     1321 cgatgtcttc atcagccgcc aggcgcgctc gctcgccgag cttccgcgtg gtgccagcct
     1381 cggcaccgcc tcgctgcgcc ggcaggcgat cgccaagcgg gcgcgtcccg atctccgcat
     1441 tttgccgttg cgcggcaatg ttgagacgcg gttgcgcaag ctcgatgcgg gggaagcgga
     1501 tgccacgatc ctcgcgctcg ccggcctaaa gcggctggga cttgctgatc aagccgctgc
     1561 ggtcctcggc gcggacgaat tcctgccggc ggtgggccaa ggtgcgatcg gcattgaggc
     1621 gcgggaagac aacgcaggcg tccgcgacat tctcgcttgt atcgatcacg gtgaaacatc
     1681 aacggcggtc gcttgtgagc gtgcgtttct tggcgcgctc gacggctcgt gcaagactcc
     1741 gatcgccggc cacgccacgg tatccggtaa tgccgtgcag ttccgcggat tgatcgcgcg
     1801 tccggacggg agcgccgcct acgacatcgc cggaaccggc aagcgcaagg acgccgccac
     1861 catcggcgcc gacgccggcc gcgagctcaa gcaccgcgct ggcccgggat tctttgattg
     1921 agtcatgcgc atcgtcgtca cccgcccgca atccgacggt gagcgcaccg cagccgcgct
     1981 ggaagcgctc gggcacgagg tgctggtcgc gccgttgatg cggattgagc cggtcgctgc
     2041 ggatcttgac ggagcgtgga gcgcaattgc cgtcacgagc gcgaatgcgc cgtggacggt
     2101 gaccggcacg agattgaaga ccttgccgat gtttgcggtc ggtcagcgca gcgcggaagc
     2161 agcgcggcgg gcaggatttg gcgatgtaac gtccgcgaac ggagatatta tggctctggt
     2221 ccggttgatc gccgcgcgcg ccgccggcgc aaaagtgcca ctgctttacc ttgcaggcga
     2281 ggatcgctcc ggcgatcttg tgacgcagct tgcggcgcac ggcgtcgaag ccgaaatgcg
     2341 gatcgtgtat cgaaccgtcg ccgaggtctt tccgccggtc ctggcagccg cgctcgaggc
     2401 gggggacgtc gacgcggtat tgcatttttc gcgccggagt gccgagctct ttgtcgaagg
     2461 cgccaaagca tcgggtgtcg ccggctcggc ggaggatgtg cggcattttt gcctgtcggc
     2521 gcaaattgcc gagccgcttg ccggcgcaag cagcgttgcc atcgcccccc gggccgatga
     2581 agcggctttg atcgcacttc ttccg
//
LOCUS       DASSRL010000213         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1615424, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000213 DASSRL010000000
VERSION     DASSRL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615424"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1595)
                     /locus_tag="VFL49_04410"
     CDS             complement(57..1595)
                     /locus_tag="VFL49_04410"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET7911248.1"
                     /translation="MNPMIPATPKRKGRSARRLVLLASAAGLAIAVTAAGQSGFGPAT
                     IPSATSIAHADGVQRLAGFADVVDKVKPAVVSVRVKMDETAKTASMNDGDMNPMQPGL
                     PLEKFFEQFGGRGMPQGLPKGIPHGRRPVTGEGSGFFISADGYVVTNNHVVDGARTVQ
                     VTTDDGTAYKAKVVGSDPKTDLALIKVEADKSFPFVKFASRDPRVGDWVVAVGNPFGL
                     GGTVTAGIVSARGRDIGSGPYDDYIQIDAPINKGNSGGPAFNMDGEVIGVNTAIFSPS
                     GGSVGIGFDIPAGTAKMVVAQLKEHGHIDRAWLGVQVQPVTDEIADSLGLKKAEGALV
                     AQPQADSPAAKAGILAGDVITEVDGVKIKDSRDLARKVGMSAPGSSVKIAILRKGEAK
                     TLSVALGKMPNEQQAKAETNDEGAANDSPRLGLTLAPAKDVAGSGDQGVAVVNVDPDG
                     PAAERGVRTGDVILDVGGKAVTNVADVRKALSDARTAGKSTVLLRLKTAEATRFIALP
                     IKKA"
     gene            complement(1971..>2815)
                     /locus_tag="VFL49_04415"
     CDS             complement(1971..>2815)
                     /locus_tag="VFL49_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET7911249.1"
                     /translation="VGKTGFLRLGFELRGKQTILADLDRRAPYMAQRALYCDDSAPGL
                     AHVFMITTTGCVLQGDRLALEIELGPRAQAHVTTQSATKIHTMDANYAAQTQTITLDE
                     ESYLELLPDPLIPHRRARYLSDTRICIPTSATLLFSEIIQPGRKHHHPDECFGATLLS
                     IAAAASRPDGQLLFTEKLIIDPQRYSMRQTGVMDSFDVFGNVILCTPKDKAESIYAHI
                     QPDVDLTRGVAWGACRLPNDAGLIFKIVGRECAQVKAKIREIWGLVRKELTGADLPQP
                     FLWR"
BASE COUNT          546 a          866 c          816 g          587 t
ORIGIN      
        1 acccatctcc cagttcattt gttccggccg cacggcggac gaaacgtgct tttttgtcag
       61 gcttttttta ttggcagcgc aatgaacctc gtcgcttcgg cggtcttcag ccgcagcaga
      121 acggtgctct tgcctgcggt acgcgcgtcg ctgagagcct ttcgcacgtc ggcaacgttt
      181 gtgacggctt tgccgccaac atcgagaatc acatcaccag tcctgacgcc gcgctccgca
      241 gcaggtccgt cgggatcgac gttcaccact gcgacgcctt gatcgccaga tcctgcaacg
      301 tctttggccg gggcaagcgt cagcccgagg cgcggcgagt catttgccgc cccctcatca
      361 tttgtttccg cttttgcctg ctgctcattc ggcatcttgc cgagggccac agacaaagtc
      421 ttggcctcgc ccttgcggag gatggcgatc ttgacgctgg agcctggcgc gctcattccg
      481 accttgcggg caagatcgcg tgaatccttg atcttgacgc catcaacttc ggtaatcacg
      541 tcgcccgcga ggatgcccgc tttggcagcc gggctgtcgg cttgcggctg agccaccagc
      601 gcaccttccg ccttcttcaa gccaaggctg tcggcaatct cgtcggtgac aggctgaacc
      661 tgtacaccca gccaggcgcg atcgatgtga ccatgctcct tgagctgggc gaccaccatc
      721 tttgcggtac cggccggaat atcgaagccg attccgaccg acccgcctga cggcgagaaa
      781 atggcggtat taaccccgat gacctcaccg tccatattga acgcagggcc gcccgagttg
      841 cctttgttga ttggcgcatc gatctgaatg tagtcgtcgt aaggaccgga tccgatgtcg
      901 cggccgcgcg ccgacacgat gcccgcagtg acggtaccgc caaggccgaa cggatttccg
      961 acagccacaa cccaatcccc gacgcgcggg tcacggcttg cgaactttac gaagggaaag
     1021 cttttgtccg cctcgacctt gatcagggca aggtcggttt tcggatcgga tccaacgact
     1081 ttcgccttgt aagccgtgcc atcgtcggtg gtgacctgca cggtcctggc gccgtcgacg
     1141 acgtggttgt ttgtcaccac gtaaccgtcg gcggaaataa agaaccccga gccctcgccg
     1201 gttaccggcc gccggccatg cggaattccc ttgggcaggc cctgcggcat gccgcgaccg
     1261 ccgaactgct cgaagaattt ttcgagcggc aacccaggct gcatcggatt catgtcgccg
     1321 tcgttcatgc tcgccgtctt cgccgtttca tccattttga cgcgtacgga aacgacggcc
     1381 ggcttcacct tgtcgacgac gtcggcaaac ccggcaagcc gctgcacacc gtcggcatgt
     1441 gcgatagacg tcgccgatgg gatagtcgca ggaccaaaac ctgactgccc ggcagcggtc
     1501 acggcgatag caagaccagc agccgatgca agaagcacga gccgacgcgc ggaacggcct
     1561 ttacgtttcg gcgtggcagg aatcatcgga ttcatctcgt aatctccact gagagtgtgt
     1621 tcagagcgat cggcctgctg attaggcaac cgcacattac ggcaatcttt ctcgataatt
     1681 gaaagttcgt aatccggcag ggcgaagccg ttttgccgga tcaaatgcag cctcacaggc
     1741 cgataacaag cctgtgaagc gccggggcat cgacggtatt tgttcttatg agagccggac
     1801 gcagccagcg gtcaaaattg gcagcactga ctatgctgcc ggccgcaaac agccggcagt
     1861 tcgacggatt gcaagctcac agcctttccc agcctgccaa aagttccttc attatcaact
     1921 cctgcaatgc agtgaactgt aatttggcga tgtgtccgtg ctcgtttttt tcagcgccag
     1981 agaaatggtt gcgggaggtc tgcgcccgtt agctccttac gaacaaggcc ccaaatctcg
     2041 cgtatcttgg cctttacctg cgcgcactcc cggccgacga tcttgaagat gagcccggca
     2101 tcgttgggca accggcaggc gccccaggcc acaccgcgcg taaggtcgac gtcgggctgt
     2161 atgtgcgcgt aaatactctc ggctttgtct ttcggcgtgc aaaggatcac attgccgaat
     2221 acgtcgaacg agtccatcac accggtctgc cgcattgaat atcgctgcgg gtcgatgatc
     2281 agcttttcgg tgaagagcaa ctgaccgtcc gggcgagagg cagccgcagc gatcgacagc
     2341 aacgttgcgc caaagcattc gtccggatga tgatgcttcc gccccggttg aatgatctct
     2401 gaaaacagca gcgttgccga cgtcggaata cagatccgcg tatcactgag gtagcgtgct
     2461 cgccggtgcg gaatcagcgg atcaggaagc agctccagat aggattcctc atcaagcgtt
     2521 atcgtctgag tttgtgccgc atagttggcg tccatcgtat gaatcttggt cgcggattgc
     2581 gtggtcacgt gcgcctgcgc ccgcggcccg agttcgatct cgagcgcaag gcggtcgccc
     2641 tgcaagacac aaccggtcgt ggtgatcatg aagacatggg caagccccgg cgcgctgtcg
     2701 tcacagtaga gcgcgcgttg ggccatgtac ggcgcgcggc ggtcgagatc cgcgaggatc
     2761 gtttgcttgc cgcgcaattc gaagccgaga cgcaggaagc ccgtcttgcc caccg
//
LOCUS       DASSRL010000214         6017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_600493, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000214 DASSRL010000000
VERSION     DASSRL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600493"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFL49_04420"
     CDS             <1..1215
                     /locus_tag="VFL49_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7911250.1"
                     /translation="ALMQMGNTLQGTLLSVRGSTEGFSPAEIGIIGACFWGGIVLGTL
                     RSEPVIRRVGHIRAFAALGAIASTIPLLHLLLIHPIAWMVLRAATGFCFAALFIVIES
                     WLNGFATNKTRGQVLSIYGMTGLIAGIGGQLLLPATDPAGFRPFCVVAITIALSLVPI
                     ALTSTKAPSSADGNVKIDPRALYRQSPFGVVAAFLSGATTGAFFALGPVFAQGRELDT
                     SEIALFMSSGTLGGFAMALPLGWLSDRFDRRLVIIAAAVIAAGMLLVMVATVPHGAYP
                     WVLYLCVAMFGATVVPIYSIILAQVSDVVSQDELVAASGGLLLLQGIGATAGPVVAGL
                     AMSASPRGLSYTLVVAQILIALWGLYTLLLRRAAPAPAQKTKFQVEPPVPVGTTLASA
                     HSTKIRRSKSRR"
     gene            complement(1198..4296)
                     /locus_tag="VFL49_04425"
     CDS             complement(1198..4296)
                     /locus_tag="VFL49_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7911251.1"
                     /translation="MVSSLLHPLNIFILGLGAGFLIPLLYRLGSSWVTAVFVLSLAAM
                     VLISGHAALMLLHDAAPIEILTGGTKPPYAINLRMGLGEAIVAFCVNLTALFGAVYVA
                     REKYGVLLLYLLLVMGIQGMIMTRDLFNLFVFLEIVSIATYGLLGLRDAPAALSATFK
                     FLMATVLASTFFLIGTVLLYAVTGVLNIDDLIADRDTIGGPIGFAALMFLLACLLLEL
                     KPFPANGWGLDVYETARSDIAAMISGGVSAGVFYALLKLLPLFDDQLEVIAILGAVTF
                     VLSNLIGLKQTKAQRLLGYSSVGQIALLTIAVSLLYGMGAEDAVPLVAGGLFVNHLFA
                     KVGFFWLAGYVGRERLEDWSILAGKSGAVFLFGTLIVAISGLPPFPGFWAKWQFIMTL
                     ASGDQYVWIAILLVGSLLESAYMFRWLGLTVHSSAGTEKTAGRPVELVPVAAMTLLLA
                     LSGYIAADLAGFTAPWMYLPVLAGLAVWLLGPLPGRAQGLIALLLVLAGSIWLISDLS
                     GLNHLFAVVLIAGGLVVSIACLNRTDRRPGFYPFLAVMLLSVAALPCATTSLEFIFAW
                     ELITLSSYFLILRRADAAPHALQYLLFSLVAAFFLLCGFAVLQAQTNSISLSALRTVG
                     PDSAAVFVLLAIGLLIKAGAAGVHVWLPGAYAQADDDVSALLSAVISKVAMFGLLVGT
                     YVAVRSEVSLNLVHVLGWIGMLTTLVGAMLAVSQNDMKRMLAYSSMSQLGYIVAAIAL
                     MSHLGWVTALYLVANHLMVKGILFLVVAGLVIRTGTRHFDDLGGLARVMPFTFASATI
                     AIVAMSGLPPLAGFGGKWLLLSAMMEKGWYGPAVMTLLATFVGFLYMEHFIHAIFFGP
                     RKPKHRDLPEAPLALLIPQYLLIAGILVMSFFPKLLIEPVSQAIDPQFASTLVWQGMS
                     LEMIYGYWNPFPVMMFAVIVSAMLFGLFWLVRNAGWLRIGVARAGLYRFFMAVFTTLT
                     PPVATAFWGGLTDVTTSVAQRTRMIYTGNAQTYNLYILYYFIVLYVACGGLANWSLTP
                     GF"
     gene            complement(4290..4649)
                     /locus_tag="VFL49_04430"
     CDS             complement(4290..4649)
                     /locus_tag="VFL49_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET7911252.1"
                     /translation="MISHIVLVTGLLLILIGLWGLLTHRNILRIIISFSLMGTGTHIV
                     TVAIGYVRNGTAPIIDRTMDASDAVSRVVDPIPSALVVTAIVIGMAVTAVMLAFAIRL
                     YNSRKTLSIDAFTDSKW"
     gene            complement(4662..5369)
                     /gene="mbhE"
                     /locus_tag="VFL49_04435"
     CDS             complement(4662..5369)
                     /gene="mbhE"
                     /locus_tag="VFL49_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HET7911253.1"
                     /translation="MIQRLFVAVVVGLFGLVFASLAIGYTEQYSLRPLAESYVRLVPH
                     ELGAPNVITGILLTYRAFDTLGEVAVLFMVAASVGLVLGTRRARGRASIKDPDEARPS
                     SEIVRTGAEILVPLICIFAAYIIMNGHLSAGGGFQGGAVIASGVLLTLLAFPQYQPNL
                     EFLSITESVAGALFVLVGIAGLIVAGGFLDSRVFPLGQFGAFFSAGAIPLLSVLLGVK
                     VGCELSVILERFRPQKP"
     gene            complement(5366..5704)
                     /gene="mnhG"
                     /locus_tag="VFL49_04440"
     CDS             complement(5366..5704)
                     /gene="mnhG"
                     /locus_tag="VFL49_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HET7911254.1"
                     /translation="MMELIGSILVLLGAVFHFSAGLGMLRMPDAYTRMQAGTKASTLG
                     NTLVLAGLAIYHPGWGLKLLIIVYFVLMTNPVSSHALARAAYGIRIPMAGDTGIDALR
                     DAKRKPGGRG"
     gene            complement(5701..5943)
                     /locus_tag="VFL49_04445"
     CDS             complement(5701..5943)
                     /locus_tag="VFL49_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase subunit MbhD domain-containing
                     protein"
                     /protein_id="HET7911255.1"
                     /translation="MLELFVYLLSGSILVSGILAIRLKSTLAAAVSGGLASLFAAVCY
                     VVLGAPDVAMTEASIGSGLSILIFLYAIRRTTEKTP"
BASE COUNT         1443 a         1799 c         1766 g         1009 t
ORIGIN      
        1 gcgctcatgc agatgggcaa tacgctgcaa ggcacattgc tgagcgtgcg cggaagcacc
       61 gagggttttt cgccggccga aataggaatc atcggggcat gcttctgggg cggaatcgtc
      121 cttggaacgc tccgtagcga acctgtcatc cggcgcgtcg gtcacattcg ggctttcgca
      181 gcgctgggag cgattgcatc aacgatcccg ctccttcacc ttctcttgat acacccgatt
      241 gcatggatgg tcttgcgcgc tgccaccgga ttctgttttg ccgcgctgtt catcgtcatc
      301 gagagctggc tgaacggatt cgcgacgaac aaaacccgag gtcaggtctt aagcatctat
      361 ggcatgaccg ggctgatcgc cggcattggt ggccagctgc ttctgcccgc aacagatccc
      421 gcaggcttcc gccccttctg cgttgtggca attacgattg cgctctcgct ggtcccgatt
      481 gcgctgacca gcacgaaggc cccatcgtcc gccgacggca acgtgaaaat cgatccgcgt
      541 gcgttgtatc ggcagtcgcc tttcggtgtc gtggctgcat tcctgtcggg cgcgaccacc
      601 ggcgcattct tcgctttggg accggtcttc gcacaggggc gtgagctcga taccagcgaa
      661 atcgcgctgt tcatgtcgag cggcacactc ggcggcttcg ccatggcgtt gccgctgggc
      721 tggctctcgg accgcttcga ccgccgactg gtgatcatcg ctgcagctgt tatcgccgcc
      781 ggaatgctgc ttgtcatggt cgctaccgtg ccgcacggcg cgtatccatg ggttctgtat
      841 ctttgcgtcg caatgttcgg ggctacggtc gttccgatct acagcatcat tctcgctcaa
      901 gtcagtgacg tcgtatcaca ggatgagctg gtcgctgcat ctggtggcct gttgcttctc
      961 cagggcatcg gtgcaacggc tggtccggtt gtcgcaggtc tcgcaatgtc ggcgtcgccg
     1021 cgcggtctgt cttatacgct cgttgttgcc cagatcctga tcgccttgtg gggcctatac
     1081 acgctgcttc tgcggcgtgc cgcgccggcg ccggctcaga aaacaaagtt ccaggtggag
     1141 ccgcccgttc ctgttgggac gacgcttgcc tcggcacatt cgacgaaaat ccggcgctca
     1201 aaatccaggc gttagactcc aattcgcgag cccgccgcaa gcgacgtaca aaacaataaa
     1261 atagtacaag atgtacaaat tgtacgtttg ggcattgccg gtgtagatca tccgcgtacg
     1321 ttgcgcgacc gacgtagtga cgtccgtcag cccgccccag aatgcggtgg cgaccggcgg
     1381 cgtcagtgtt gtgaagacag ccatgaagaa acgatacagg ccggcacgcg cgactccgat
     1441 tcgcagccag ccggcgttcc gcaccaacca gaacaagccg aacaacatgg cggaaacgat
     1501 caccgcaaac atcatgaccg ggaacggatt ccagtagccg taaatcattt ccagcgacat
     1561 gccctgccac accagggtcg acgcgaattg cgggtcgatt gcctgggaaa ccggctcgat
     1621 cagcagtttc gggaagaacg acatcaccag gatgcccgcg atgagcagat attgcgggat
     1681 cagcaaggcg agcggcgctt ccggcaagtc gcgatgcttc ggcttgcgag gcccgaaaaa
     1741 gatcgcgtgg ataaaatgtt ccatgtagag aaagccgaca aaggtcgcca gcagcgtcat
     1801 cacggccggc ccgtaccagc ctttttccat catcgcgctc aacagcagcc atttgccgcc
     1861 gaatccggcg agcggcggca ggcccgacat tgcaactatg gcaatggtcg ccgatgcgaa
     1921 tgtgaaaggc ataacgcgtg caagaccacc gagatcgtcg aaatgacgcg tgcccgtgcg
     1981 aataacgagt cccgcaacaa caagaaacaa tatccccttc accatgagat ggttggcgac
     2041 aagatacagc gccgtgaccc agccgaggtg gctcatcagc gcaatcgccg cgacgatgta
     2101 gccgagctgg ctcatgctgg aataggccaa catgcgtttc atatcgttct ggctgaccgc
     2161 gagcatggcg ccgaccagcg tcgtcagcat gccgatccac ccgaggacat gaacaagatt
     2221 caagctcacc tcggaacgaa ccgccacata agtgcctacc agcaatccaa acatcgcgac
     2281 tttgctgatg accgccgaaa gcaaggcgga cacgtcgtca tcggcttgcg cataggcgcc
     2341 cggcagccag acatggaccc cggcagctcc cgccttgata agcagaccaa tcgccagcag
     2401 aacaaaaacc gcggcactgt cgggacccac cgtgcgtaat gccgacagcg atatgctgtt
     2461 ggtctgcgcc tgcagcaccg caaacccgca caacaggaaa aaggctgcaa caagcgagaa
     2521 cagcaaatat tgcaacgcat gcggcgctgc gtcggccctt cgcaggatca gaaaatagga
     2581 cgacagcgtg atcaactccc acgcgaagat gaattcaagg ctcgtggtgg cgcacggcag
     2641 cgccgccact gagagcagca tgacggccag aaacggatag aaacccgggc gtcggtccgt
     2701 acgattgagg caggcgatcg aaacgacgag accgccggcg atcaggacaa cggcaaacag
     2761 atgattgagg cccgacaaat cggaaatgag ccaaatactc cccgccagaa ccagaaggag
     2821 cgcgatgaga ccctgtgccc ggcctggcaa tgggccgagc agccacacgg ccaatcccgc
     2881 aaggaccggc aaatacatcc agggcgcggt gaaaccggca aggtcagcag cgatataacc
     2941 gctgagtgcg agcaataagg tcatcgcggc gaccggaacg agctcaaccg gccggccggc
     3001 ggtcttctcc gtcccggcgg acgagtgcac agtgagaccc agccagcgaa acatgtaggc
     3061 cgactccagc agcgagccga caagcaaaat ggcgatccac acgtactgat cgccggaagc
     3121 gagcgtcatg atgaattgcc atttcgccca gaagccgggg aatggcggca ggccggaaat
     3181 tgcgacaatc agcgtcccga atagaaaaac tgcgccggac tttccggcga gaatcgacca
     3241 gtcttccagg cgctccctgc cgacataacc agcaagccag aagaagccga ccttggcgaa
     3301 cagatgattg acaaaaagac ccccggcgac gaggggaacc gcgtcttcgg cacccatccc
     3361 gtagagcaag gaaaccgcga tggtcagcaa cgcaatctgg ccgaccgagg aatagccaag
     3421 gaggcgttgc gctttcgtct gcttaagacc gatgaggttc gacaggacga atgtcacagc
     3481 cccgaggatc gcgatgacct caagctggtc gtcgaacagc ggaagcagct tgagaagagc
     3541 ataaaagacg ccggctgaaa cgccacccga gatcattgcg gcgatatcgc tacgtgccgt
     3601 ttcataaacg tcgagacccc atccattcgc cgggaacggc tttaattcca gcagcaggca
     3661 ggccagcagg aacatgagcg cggcaaaacc aatcgggccg ccgatcgtgt cgcgatctgc
     3721 gatcaggtca tcgatgttaa ggacgccggt aacggcatag agcagcacgg tgccgatcag
     3781 gaagaatgtc gaggccagca ccgtggccat gagaaatttg aatgtcgcag acaacgctgc
     3841 cggcgcatcg cgcaatccga gaaggccata ggtcgcgatg gagacgatct ccaggaacac
     3901 aaacagattg aaaaggtcgc gggtcatgat catgccctgg atgcccatga cgagcagcag
     3961 gtagagcagc aacacgccgt atttttcgcg cgccacatag acggcgccaa aaagcgcggt
     4021 caggttgacg caaaacgcga cgatcgcttc gccgagaccc atgcgcagat tgattgcata
     4081 aggcggcttc gtgccgcccg tcaaaatctc gatcggcgca gcgtcgtgca aaagcatcag
     4141 cgcggcatgg cctgagatca gcaccatggc cgccagcgaa agaacaaaaa cggccgtcac
     4201 ccatgaactg ccgaggcgat acaacagcgg aatcagaaat ccggcgccca ggcccaaaat
     4261 gaagatgttc agaggatgaa gaaggctgct caccatttcg agtccgtgaa ggcgtcaatc
     4321 gagagcgtct tcctggaatt ataaagccgg atagcaaagg cgagcatgac ggccgtcacg
     4381 gccatgccga tcacgattgc ggtgacaaca agcgcagagg gtatcggatc gacaacgcgc
     4441 gacacggcgt ccgacgcatc catggtgcga tcgatgatcg gtgctgtccc gtttctgaca
     4501 tagccaattg caacggtcac gatatgggtg ccggtaccca tcagggagaa gctgataatg
     4561 atccgcagaa tgttgcggtg ggtgagcaaa ccccagagtc cgataaggat cagcagaagg
     4621 cccgtcacca gaacgatgtg cgagatcatc acgtggggcc tttacggctt ttgaggacgg
     4681 aatctttcca ggattacgct cagttcgcag ccgaccttga cgccgagaag aaccgataaa
     4741 agaggaatgg cgccggcgct gaagaaggca ccaaactgtc caagcggaaa tacacggcta
     4801 tccagaaagc cgccggctac gatgagcccg gcaatgccga cgagcacgaa gagtgcaccg
     4861 gcgaccgact ccgtaatgct gaggaattcg agattgggct ggtattgcgg aaaggccagt
     4921 aatgtcagca ggacgccgga ggcgatcacc gcaccgccct ggaagccgcc gcccgcggac
     4981 aggtgaccat tcatgatgat ataggccgca aaaatgcaaa tgagcggaac aagaatctcg
     5041 gctccggttc gcacaatctc cgatgacggc cgcgcctcgt cgggatcctt gatggacgcc
     5101 ctgccccgcg cacgtcgcgt acccagcacg aggccgacgc ttgccgccac catgaacagt
     5161 accgcgacct ctccgagcgt atcgaaggcg cggtaggtca aaagaattcc ggtgatgacg
     5221 ttcggtgcgc caagctcgtg cggaacgagc ctgacatagc tttccgccaa aggccgcagc
     5281 gaatattgct cggtatagcc gatcgcaaga cttgcaaaca caagtccgaa caagcctacg
     5341 accacggcga cgaagaggcg ctgaatcacc cgcggcctcc aggttttctt tttgcatcgc
     5401 gcagcgcgtc tataccggtg tcgcctgcca tcggaatccg gatgccgtac gctgcccggg
     5461 ccagcgcgtg cgacgacacc ggatttgtca tcagcacaaa atagacgatg atcaaaagct
     5521 tcagccccca tcccgggtga tagatcgcaa gacccgcgag aacgagggta ttgccgagcg
     5581 tggaggcctt ggtgccggcc tgcatgcgag tataggcatc gggcatccgc agcatcccga
     5641 gccccgccga gaagtgaaag accgcgccaa gcagcaccag gatgcttccg atcagctcca
     5701 tcatggcgtt ttctctgtcg tcctgcggat cgcataaagg aaaatgagaa tcgacaggcc
     5761 ggagccgatc gacgcctccg tcattgcgac gtccggcgcc cccagcacga cgtaacagac
     5821 cgcggcaaac aggctggcca gcccgccgct gacggcggcc gccagcgtgc ttttgaggcg
     5881 gatggccaga atgccgctca ccagaatgct gccgctcagc aagtagacga agagctcgag
     5941 catgtggtca gagcccttgc tcgagatagc gggcaatcgc gacaacagcc agaaaactca
     6001 gaacgccgta aaccagc
//
LOCUS       DASSRL010000215         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1457836, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000215 DASSRL010000000
VERSION     DASSRL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457836"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFL49_04450"
     CDS             <1..212
                     /locus_tag="VFL49_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7911256.1"
                     /translation="AKAFGNDVGGGRVVIRQKLSQSDVAAMAGIARENVSRILNEWMR
                     SKTLTRLSGYYCVENKDKLEREIKL"
     gene            347..514
                     /locus_tag="VFL49_04455"
     CDS             347..514
                     /locus_tag="VFL49_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911257.1"
                     /translation="MRKTIILAAATMFLACVTVVTGVARTFDVPSTALASLVVSPHTI
                     GSLTARILNAI"
     gene            complement(625..>3297)
                     /locus_tag="VFL49_04460"
     CDS             complement(625..>3297)
                     /locus_tag="VFL49_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7911258.1"
                     /translation="SLLNLLKHRKERGSFGGDPEDYHAKILAQIAKRTLTKQNVPTPA
                     GRIIQIVNQPMPDGGWVATHEDITDKIEAETAIKKQKEQLDAALANISQGVCMFDASK
                     RLIICNKQYADIYELSEELTKPGTTFKAILEHTIARGNGPKDSAAYLKTRLTDVAGRV
                     PYQTTNKLRDGRYVAVVHRPMANGGWVATHQDITEAKHREESFRLLFEGNPVPMWVID
                     RETLRFLAVNEAAVAHYGFSREQFMAKSVADLRPAADRERFKAFIHGLSDDQFVENVG
                     QHIKADGTKIDIAVYSRALTYDGRNARLTVVHDITKTKLTEAELRRTQIFLDAVIEHV
                     PLPIVVRHVDATGNDVQGGRFLLFNRAYEELTGDSRSRLIGKTAHELFPPERADMIVT
                     SDLETLQGNKIVSTPEHYINTATNGLRLVKANKIAIRDDSGDAQYLLTVLDDITERRR
                     SEQHISYLAHNDSLTGLPNRTSFLKHLFATLDDASKNGARFAIMCLDLDRFKEANDTY
                     GHLVGDSLLREAARRLQAAAEGAFLARIGGDEFIIVLSSDDAEAAAAVIGERMMESFK
                     ENFDVDGHQIQVGLSVGVAMYPADGADATALIANADAALYQAKAEVRGSVRFFEEKLG
                     SRLRERRELQTDLRSAPDRGDLFLHYQPQKTIAYGKVVGFEALARWRCPKRGWVSPET
                     FIPIAEQSTYIITLGEWILREACKEAASWPVPLRVAVNISPVQFHHGDLAKLVHSILL
                     ETGLKPDRLELEITEGVLIDDFSRAVSILRKLKLLGVHIALDDFGSGYSSLSYLHAFP
                     FEKIKIDRVFVGDLENNHHSMAIVRAIVTLGHSLNIPVLAEGVENESQLSFLAQEGCD
                     EVQGYLTGKPQPIETYADLVGKVIAKPRQKALAG"
BASE COUNT          597 a          979 c         1037 g          684 t
ORIGIN      
        1 tggccaaggc gtttggaaac gatgtcggcg gcggccgcgt ggtcatccga cagaagctga
       61 gccagagcga cgtagcggcg atggcaggaa tcgcgcgcga gaatgtcagc cggattctca
      121 acgagtggat gcggtcgaaa acactgaccc gcctgtcggg ctattactgc gtcgagaaca
      181 aagacaagct cgaacgagag ataaaactat agcgatttca gcgcctttta ggcctggcaa
      241 aaaaagcgct cttctgtgac gcgggtcaca tcggcatgaa gcgcggcctc atagtgtgat
      301 ggccataggg agccggttgc ttccgcatta ttcagggcca acgaccatgc gtaagaccat
      361 catcctcgcc gcggccacca tgttcctcgc ctgcgtaacg gtggtgacag gcgttgcgcg
      421 cacgttcgac gtcccttcca cggctttggc gagcctggtg gtaagtcccc acaccatagg
      481 cagcctgacg gccagaattt tgaacgcaat ctgaccgtcg taaggcctgc cccccaccca
      541 aggggcaggc cccccgtttc acgatcctcc aaacttcctg gcggcctccc ggaggccgct
      601 tcttttttga gggtatccgc ggcgttatcc tgccaatgcc ttctgacgcg gcttcgcgat
      661 caccttgccg acgagatcgg cataggtttc gatcggctgc ggcttgccgg tcaaatagcc
      721 ctgcacctcg tcgcaacctt cctgggcaag gaatgacagc tggctttcgt tttcgacacc
      781 ttcggcgagt accgggatat tgaggctgtg ccccagcgtg acgatcgcgc ggacgattgc
      841 catcgaatga tgattgttct cgaggtcgcc gacaaagacc cggtcgatct tgatcttctc
      901 gaacgggaag gcgtgcagat aggacagcga ggaatatccg gacccgaagt cgtcgagcgc
      961 aatatgcacg ccgagcagct tgagcttgcg cagtatggat acggcgcggg agaaatcgtc
     1021 gatcagcacg ccctcggtga tttccagctc cagccggtcg ggcttgagtc cggtctcaag
     1081 aaggatcgag tgcacgagct ttgccagatc gccgtgatgg aactgaaccg gagaaatatt
     1141 caccgcaacc ctgagcggga ccggccatga ggcagcttcc ttgcaggcct cgcgcaggat
     1201 ccattcgccc agcgtaatga tgtacgtgct ctgctcggcg atcggaatga aagtttccgg
     1261 cgatacccag ccccgtttcg ggcagcgcca tcgcgccagc gcctcgaagc cgacaacctt
     1321 gccgtaagcg atggtcttct gcggctggta gtgcaggaag aggtcaccgc ggtccggcgc
     1381 gctgcgcaga tcggtctgca attcccggcg ctcgcgcagc cgcgaaccga gcttttcctc
     1441 gaagaagcgc acggaaccgc gcacctccgc ctttgcctga taaagagcgg cgtccgcgtt
     1501 ggcgatcaag gccgtcgcat cggcgccgtc ggcagggtac atcgccacgc cgacgctcaa
     1561 gccgacctgg atctggtgcc cgtcgacgtc gaaattttcc ttgaatgatt ccatcatgcg
     1621 ctcgccgatc acggcggccg cagcctccgc atcgtcgctg gacaacacga tgatgaactc
     1681 atcgccgccg atgcgggcga ggaacgcacc ttcggcggcg gcctgcagcc ggcgggcagc
     1741 ttcgcgcagc aggctgtcgc caaccagatg gccatatgtg tcgttggctt ccttaaaccg
     1801 atcgagatcg agacacatga tcgcaaagcg ggcaccgttt ttcgatgcgt cgtcgagtgt
     1861 cgcaaacagg tgcttcagga atgacgttct gttcggcagc ccggtcaatg agtcgttgtg
     1921 cgcaagatac gagatgtgct gctcgctgcg ccggcgctcg gtgatgtcgt cgagcaccgt
     1981 caggaggtat tgggcatcgc cgctgtcgtc gcggatggcg atcttattgg ctttgaccag
     2041 gcggagcccg ttggtcgccg tgttgatata gtgctcgggc gtggagacaa tcttgtttcc
     2101 ctgcaatgtc tccaggtccg acgtgacgat catgtcggcg cgttcgggcg ggaacagctc
     2161 atgcgcggtc ttgccgatca gccggctccg tgaatcgccg gtcagctcct cgtaggcccg
     2221 attgaacagc aggaagcgcc cgccttgcac gtcgttgccc gtggcatcga catgacggac
     2281 cacgatgggg agggggacat gttcgatgac cgcatccagg aagatctggg tgcggcgaag
     2341 ctcggcctcg gtcagtttcg ttttcgtgat gtcgtggacg acggtcagcc gggcgttgcg
     2401 tccgtcatag gtgagggcgc gcgaatagac cgcgatgtcg atcttcgtgc cgtcggcctt
     2461 gatatgttgc cccacgtttt cgacgaactg gtcgtccgaa agtccatgga tgaaggcctt
     2521 gaagcgctcg cgatcagcgg ctggccgcag gtcggcgacc gactttgcca tgaactgctc
     2581 gcggctgaag ccgtaatgcg cgaccgcagc ctcgttgacc gcgaggaaac gcagcgtttc
     2641 gcggtcgatg acccacatcg gcaccgggtt tccttcgaac aggaggcgga aggactcctc
     2701 gcggtgcttg gcctcggtga tgtcctggtg cgtcgccacc cagccgccgt tggccatcgg
     2761 ccgatgcact acggcgacgt aacgcccgtc gcgcagcttg ttggttgttt gatagggcac
     2821 gcggcctgcg acgtcggtca gacgagtctt gagataggcc gcggaatcct tgggcccgtt
     2881 tcccctggca attgtgtgct cgaggatggc cttaaaggtc gtgcccggct tggtcagctc
     2941 ctcgctcaac tcataaatat cggcgtattg cttgttgcag atgatcagcc gcttcgaggc
     3001 gtcgaacatg cagacgccct gtgaaatgtt ggcgagcgcc gcgtccagct gttccttctg
     3061 cttcttgatc gcggtctcgg cttctatctt gtcggtgata tcttcgtggg tggctaccca
     3121 gccgccgtca ggcatcggct ggttgacgat ctggatgata cggcccgccg gagtgggcac
     3181 gttctgcttg gtgagcgtcc gcttggcgat ctgcgccagg atcttggcgt gatagtcctc
     3241 ggggtcgccc ccgaacgagc cgcgctcctt gcggtgcttc agcaggttga ggaggga
//
LOCUS       DASSRL010000216         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1247970, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000216 DASSRL010000000
VERSION     DASSRL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247970"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFL49_04465"
     CDS             complement(<1..196)
                     /locus_tag="VFL49_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911259.1"
                     /translation="MPPPSAAADGPFAEFSGSWSGSGTLRPENGAAERIRCNANYRQG
                     SAPNEINLQLRCASDSYNFDL"
     gene            complement(555..932)
                     /locus_tag="VFL49_04470"
     CDS             complement(555..932)
                     /locus_tag="VFL49_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911260.1"
                     /translation="MVDELIPADVRDFILRHIDSVAQWEALLLLRANPNETWDVSRIA
                     RRLYTSDKEIAELLAQLCQDGLLTVSDGLFRYECASAENRDIIDRLAAIYARHLIPVT
                     NMIHDKPRRIRRFAEAFKFRKAR"
     gene            complement(1148..1603)
                     /locus_tag="VFL49_04475"
     CDS             complement(1148..1603)
                     /locus_tag="VFL49_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911261.1"
                     /translation="MMRAGIAAVVLAGAVIVTAAAPGMAQVRDAVYRGTLVCDKLPFS
                     AGKGREAIEVTIAGGIVRYSHVVRLREAAEPVPEQGRGSLNGQDIALEGSWKSGNRQF
                     EAKYSGAFVRRHADLKGTQTWTDGGRTFTRACAGTIKRPLRVFLPGEKK"
     gene            complement(1714..2547)
                     /locus_tag="VFL49_04480"
     CDS             complement(1714..2547)
                     /locus_tag="VFL49_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7911262.1"
                     /translation="MFTANLDSTIERAYDFVAQASWIDRLITHLGFDPNQLTTDGVFH
                     HLMDIAVANINTANMLALGGAIFYVATLMMRTIVPLRVIGIISIAFFIAYGVVAGAVS
                     TLLLYLFSLPVNIVRLRQMLTLVRKARSSAQGDLSMDWLRPYMTPRRYKKGDILFRKG
                     DVANEMFLTVTGKFLVTEIGVELPPGRLMGELGFAAPKNRRTQTIQCLESGDVLSITY
                     ETLLELYFQNPEFGYYLLSLTSERLLQNISRLEGALEDCKVKVQQLEAQRSGLPAAAP
                     R"
BASE COUNT          518 a          808 c          779 g          511 t
ORIGIN      
        1 tgaggtcgaa attgtagctg tcgctggcgc agcgcaactg aagattgatc tcgttcgggg
       61 cgcttccctg gcggtagttg gcattacagc ggatacgctc ggcggcgccg ttttcgggcc
      121 gcagcgttcc gctaccgctc cagctgcccg agaattcggc aaaggggcca tcagcggcag
      181 cgctgggagg cggcatgaca atagccgcaa ccagggcaca ggcgggaatg gcagtgcgta
      241 cgagcgcgcg ggaaattcgg gttggcaagg gacgtttccc tttcaggttg ggttgaactg
      301 cccaaatggg ggccgccggc ctcaaatcaa atggggtagg tccggttcca atcaagcatg
      361 cccgttcagc gcgtcggaag tctagcggca attctctcgc caatggagcg ccatccccgg
      421 cgcggcccgg tcacttcctc tgatgaacga attaccgccc ggccttcatc gttccgctca
      481 ttccctgctc cagaacatcc cataaagcag caagccattg cgaccgacgc aaacccacgg
      541 ttgcctggac cagcttatcg agccttcctg aacttgaatg cctcggcaaa ccggcggatg
      601 cgccgtggtt tgtcatgaat catattggtc accggaatca ggtgccgcgc atagatcgcc
      661 gccagacgat cgatgatgtc gcggttctcg gccgatgcgc attcataccg gaaaaggccg
      721 tcgctgaccg tcagcaagcc gtcctggcac aactgcgcca gcaattcggc gatctccttg
      781 tcgctcgtgt agaggcgacg ggcaatcctg ctgacgtccc aggtctcatt gggattggcg
      841 cgcagaagca gcaatgcttc ccactgggca acggagtcga tatggcgaag gatgaaatcc
      901 cggacgtccg ccgggatgag ttcgtccacc atttcctgga ccagcccgct gtctggcgcc
      961 gccccaccaa gcacgcgtcg atagccgaat ccggtccgtc cagcccggat tcactgcggc
     1021 gagactactt atggagaccg gcctgtcact gtgacatcgt gccagccgtc aggcacggaa
     1081 ccttttgcca agtcgtgcca agttgcgcca ggttgtgaca ggccgtgaca agtcgcgcag
     1141 ggcgcggcta ctttttctca ccaggcagaa agaccctgag cggccgcttg atcgtgccgg
     1201 cgcacgcgcg ggtgaaggtc ctgccgccgt ccgtccaggt ctgggttccc ttgaggtcgg
     1261 cgtgccgccg gacgaaggcg ccgctgtact tcgcttcgaa ttggcggttg ccggacttcc
     1321 aggatccctc caatgcgatg tcctgtccgt tgaggctgcc ccgcccctgc tctggaacgg
     1381 gctcggctgc ctcgcgcagg cgaacgacgt ggctatagcg aacgatgccg cccgcgatcg
     1441 taacttcaat cgcctcacgg cctttgcctg cggaaaacgg cagcttatcg cagacaagcg
     1501 tgccccgata aaccgcatcg cgcacttggg ccatccccgg cgctgcggcc gtcacgatca
     1561 cagccccggc cagcacgacg gccgctattc ctgcccgcat catttacttc cccccaaaag
     1621 cttttgtcgt tgcggccgcc cggcagccgt cacaaccgtg gccgacaata ctgcacattt
     1681 tccggcggga aggcagtgga atttcgactg ccgctagcgc ggcgccgcag caggcagacc
     1741 ggaccgttgc gcttccagct gctgcacctt gaccttgcag tcctccagcg cgccttcaag
     1801 gcgtgaaata ttctgcagga ggcgctcgct ggtcaggctg agcaggtagt agccgaattc
     1861 cggattctgg aaatagagct cgagcagcgt ctcataagta atgctcagca catcaccact
     1921 ttcgaggcac tggatggtct gcgtacggcg gttcttcgga gcagcaaagc cgagttcgcc
     1981 catcaagcga ccgggcggaa gctcgacacc gatttcggtg accaggaatt tcccggtcac
     2041 cgtcaggaac atctcgttgg ccacatcacc tttgcggaaa aggatgtcgc cctttttgta
     2101 tcttcgcggc gtcatgtaag gccgcagcca gtccatcgac aggtcgcctt gcgccgaact
     2161 ccgcgccttt cgcacgaggg taagcatttg acgcaggcgg acgatgttga cgggcagcga
     2221 gaacagataa agcaggagcg tcgagacggc gcccgcgacg accccataag cgataaagaa
     2281 tgcgatgctg atgatgccga tgacacgcag cggaacgatc gtgcgcatca tcagggttgc
     2341 cacgtagaag atcgcaccac cgagcgcgag catgttcgcg gtgttgatat tcgcgaccgc
     2401 aatatccatc aaatgatgga agactccgtc ggttgtgagc tggttcgggt cgaaacccag
     2461 atgggtgatc agcctatcga tccacgacgc ctgggccacg aaatcatagg cgcgctctat
     2521 ggttgaatcg agatttgcgg tgaacatgca tcccccaccg gtggcatggg ctattcgttt
     2581 tctgcccgga taatacagcg cgttcgtacg gtaatc
//
LOCUS       DASSRL010000217         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1195624, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000217 DASSRL010000000
VERSION     DASSRL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195624"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFL49_04485"
     CDS             <1..219
                     /locus_tag="VFL49_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET7911263.1"
                     /translation="KNVANPLSLILSAAMLLAWHGRRNKAKPLEAAAAAIDKAVEEAI
                     AAKESTADIGGRLGTRQTGEAVARRLRA"
     gene            216..1070
                     /locus_tag="VFL49_04490"
     CDS             216..1070
                     /locus_tag="VFL49_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7911264.1"
                     /translation="MKARMRCDCHVHIVGPADRYPQADNRTYIADVATLKQLEMVSAA
                     RAIRRFVIVQPSFYGTDNALLLESLAALGGRGRGVAVVNPETTSARALSAMAAEGVRG
                     LRINLYSPLRETVPFAARFDAVAGSACELNWHIEVIAPLKMLAENSALLRASRVPVVI
                     DHYGLYGGMRAGQSEGIALLALVAQPHVWIKLSAPYRVSPNPLAIKPDADWLAALLTA
                     APDRCLWGSDWPHTPPHDEHMGSGVALPYRSLRYESLVDEFIAAINSDELADRILAHN
                     PARLYGFS"
     gene            1523..>2986
                     /locus_tag="VFL49_04495"
     CDS             1523..>2986
                     /locus_tag="VFL49_04495"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7911265.1"
                     /translation="MFSAEFKRAGSDLTLLGPDGRKFIVFDYFRFEKRPDLVSSDGAA
                     LSAKVVEALAQANSPSQYAQAVTPGPSGQIIGKVEKVSGSVTAVRNGVAVTLNVGDTL
                     NKADIIQTGGDSTCGISLLDGTALNLSANTRMALNEFLFEANAGTGNAGHLTLVQGAF
                     AFVSGLVAKTGGLNIETPVANIGIRGTVGGATCFEISAQARDRCEYYAAQEINGPNAG
                     QPSSFTLSTGGRYANGQYIGGSPIGTVTVGANAQVTATGVNTPPQVAFISAAAADPGL
                     SSLTQQLIQSFPTFTPGPQGPQGPQGPQAPQGPQGPQSPNPQSGPSPGSSTPPPPDNG
                     SPDTHPPLTSIPPPVQPTITAVSFVPNDPGPVASSTNAAVAADAQIIQSAAASAAPPP
                     PAPATISIAIATDSTPTVPNGGVTRDNTPTLSGSVSADVVSVEVLDGPTSLGPATVDG
                     SGSWNFTTGPLADGAHTFTAVATDSAGHTVATNPMTAV"
BASE COUNT          580 a          907 c          908 g          591 t
ORIGIN      
        1 aagaatgtcg cgaatccgct ctcgctcatt ctgtcggccg cgatgctgct cgcctggcat
       61 ggtcgccgca acaaggccaa accgttagaa gccgccgccg cagcgatcga taaagccgtc
      121 gaagaggcga ttgcggcgaa ggaatcaacc gcggatatcg gcggccgtct tggcacccgc
      181 cagactggcg aggcggtggc tcgccgtctg cgcgcgtgaa ggcacggatg cgctgcgact
      241 gtcatgttca catcgtcggc ccggcggatc gctatccgca ggcggacaac cgtacgtata
      301 tcgcggacgt cgccacgctc aagcagcttg agatggtttc ggcggcacgc gcgatcagac
      361 gatttgtcat tgtgcaaccg agcttctatg gcaccgacaa cgcgcttttg ctggagagcc
      421 tcgccgccct ggggggacgg gggcggggcg ttgcggtcgt caaccctgaa acgacatctg
      481 cgcgtgccct ttccgcgatg gctgcggagg gcgtacgcgg gctccgtatc aatctttata
      541 gcccgctccg cgaaacggtg ccgtttgcgg cacgctttga tgctgtggcc ggatccgcct
      601 gtgaactgaa ttggcatatc gaagtcatcg ccccgctaaa gatgctggcc gaaaattccg
      661 cgctgttgcg ggcctcgcgc gttccggtgg tgatcgatca ctacggtctt tacgggggca
      721 tgagagccgg tcaatccgaa gggatcgcct tgctcgcgct ggtcgcgcag ccgcatgtct
      781 ggatcaagct gtcggcgcct tatcgcgtga gcccgaaccc cctggcgatc aagcctgatg
      841 ctgattggct cgcggccttg ctgacggctg cgccggatcg ttgcctctgg ggaagcgact
      901 ggccgcatac gccaccgcac gacgagcata tgggcagcgg cgtcgctttg ccctatcgca
      961 gtcttcgata tgaaagcctg gtcgacgaat ttatagcggc aatcaattca gacgaactgg
     1021 cggatcgcat cctggcgcac aatccggctc ggctctacgg gttttcatga tcccgcagag
     1081 gacgaatagc ttggaaatac acgccccgat tgtgacttaa ccagaacggc gagttctcca
     1141 taaatttcgc gtaaaaatag cgattcagct gaccgctcat cgcgtgcagg tagtattgta
     1201 cagggccgtt gcgaagcccg ggaggaagaa aaaggcgcgc gtggcagggc tctgggggga
     1261 gtcgaccatt gcagtgtggt cggtgggact ttttgcccga tctcgggtcg ggcgggcatg
     1321 gcatgccttg tcgccttttc ttgcgcgagg cagaggcggc gttttctctc gttgcgtcta
     1381 atgggccgca cgctccacgc caggctgagc ggctacggcc ggtgcggcga tgactcgctc
     1441 gttcaatccg cccgccacgc acatacaggc gattgccaat cacgccgatg ctgtgtctct
     1501 gaccatcccg gacgcacagc tgttgttttc ggccgagttt aagcgggccg gatcagacct
     1561 caccttgctt gggcccgatg ggcgtaaatt tattgttttc gactatttcc gattcgagaa
     1621 acgacctgat ttggtctcaa gcgacggcgc cgcactttct gcgaaagttg tcgaggcgct
     1681 tgcccaggca aattcgccca gtcaatacgc gcaggctgta acgccgggtc catcagggca
     1741 gatcatcgga aaagtggaaa aggtcagcgg ttcggtgaca gccgtgcgca acggtgtcgc
     1801 cgtcacgctc aatgtcggcg atacgctgaa caaggccgac attattcaaa ctggtggcga
     1861 ttcaacgtgc ggaatcagtc tgctcgacgg aaccgctctt aacctgtccg caaatacgcg
     1921 gatggctctc aatgagtttc tttttgaggc caatgccggc acgggaaatg caggtcatct
     1981 gacgcttgtt cagggtgcat tcgcattcgt ctccggcctg gttgcaaaaa ccggcggctt
     2041 aaacattgaa acgccggttg cgaatatcgg aatccgcggg accgtcggcg gtgcgacgtg
     2101 ttttgagatt agtgcccagg cgcgagatcg ctgcgaatat tatgcagccc aggaaatcaa
     2161 cggaccaaat gctggtcagc cgagctcatt cactctttcg accggaggca gatatgccaa
     2221 cggccagtat atcggcggat caccgatcgg cacggtaacg gtcggagcga acgcccaggt
     2281 gacggcaacc ggcgtaaaca cgcctccgca agttgcattt atttcagctg ccgctgcaga
     2341 cccggggctg tcgtcgctga cgcagcagct gatccagtcc tttccgacat tcacccctgg
     2401 gccacaaggt ccacaagggc cacaagggcc tcaggcgccc caagggccgc agggcccaca
     2461 aagcccaaat ccacaatcgg gtccgagtcc cggcagttct acacctccgc cgccggacaa
     2521 tggcagcccc gacacccatc cgccgctgac gagcattcca ccgccggtcc agccgacgat
     2581 cacggccgtc agttttgtgc cgaacgatcc tggtcctgtg gcatcttcca cgaacgcggc
     2641 ggtggccgcc gatgctcaga tcattcaatc ggcagcggcg tctgcggctc cgccgcctcc
     2701 tgctcccgcg acgatcagca tagcgatcgc caccgatagc acgccaacgg ttccgaacgg
     2761 tggggtgaca agggacaata cgccgaccct gtcaggctcg gtgagcgccg atgttgtctc
     2821 cgtggaagtc cttgacgggc cgacaagcct tgggccagcg acggttgacg gatcgggctc
     2881 ctggaatttt accaccgggc cgttagcgga tggcgcgcat accttcaccg cggtggcaac
     2941 cgattcagcg ggacacaccg tcgccaccaa tccgatgaca gctgtc
//
LOCUS       DASSRL010000218         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1423129, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000218 DASSRL010000000
VERSION     DASSRL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423129"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..712
                     /locus_tag="VFL49_04500"
     CDS             128..712
                     /locus_tag="VFL49_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911266.1"
                     /translation="MGESAVAYSGVDAARETAGGALTIVNSTIIGKVRSVELILVSNT
                     IFVAALAPSDTWTAPVRSEKKQSGCVRFSYVPPGSLTPRRFRCQPDLEIATRIDATEK
                     AANGPIPQAQVNAIRADVLSWLAPSFSSLRHGAPDYAQLRSSCPAQIRAGASDESEMG
                     AFHDLFQPQRETNLKVRLEEYLRFGLEAGSFYET"
     gene            1019..>2651
                     /locus_tag="VFL49_04505"
     CDS             1019..>2651
                     /locus_tag="VFL49_04505"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HET7911267.1"
                     /translation="MSGDYSRVRFDPKNDFGAVLMQQGRVQLDSDWNELAALLDRRWR
                     AETTDIIGRGTVPKETPDGFRIQVAAGGLTIGRGRIYVDGSLAENHGKAPLEFDAVLA
                     EQRGTLAVPYNEQPYFPNVSVAAPAPTSGGPHLVYLDVWRREVTFLEDLNLVEKAVGV
                     DTTTRWQTVWQVKILANVGSNATCGSPLEAWNSLIAPSAGRLSSAGVGVPTNTDPCLL
                     PPSGGYRGLENRLYRIEIHDGGPSGTATFKWSRDNASVATSVTAITALDKLTVASVGR
                     DSVMHFKIGDWIEVTDDWLEFARQPGVSPQPAGVMAQIKNIDDANRIITLVSPIPANV
                     FGTDAQGNTDPARHTRVRRWDQSGQVRDTNGNLLVDLNAPGAKGVIPVPATGTSIVLE
                     DGVQITFDTPAGGNYKIADCWNFAARTADASVEELKQAPPLGVHHHFCRLALVTFPSS
                     VTDCRTLWPPEVAGEGCECTVCVSAASHNQGTLTIQMAVEQVKAAGGGTICLGVGTYN
                     LGEAPMPINITGARSLTLRGQGTQTSLIYAGRGTAL"
BASE COUNT          538 a          797 c          853 g          463 t
ORIGIN      
        1 caccagccag ccgagcctga tcgtacagat tcccaacgtc gaggtggaga tcgatcactg
       61 catcatcggc gcgctgcaag tcgccgatgg ttcacaagtg acgatcaaca attccatcgt
      121 cgatgccatg ggcgaaagcg cggtggctta ttccggcgtc gacgccgcgc gcgaaacggc
      181 gggcggcgcg ctgacgatcg tgaacagcac gatcatcggc aaggtgcgct cggtcgagct
      241 gatattggtg tcgaacacga ttttcgtcgc ggcgctggcg ccaagcgata cctggacggc
      301 gccggtgcgc tcggagaaaa aacagagcgg ctgcgtgcgc ttcagctatg tgccgccggg
      361 ttcattgacg ccgcgccgtt ttcgctgcca gcccgatctg gaaatcgcca ctcgtattga
      421 cgccacggaa aaggctgcca acggtccgat cccgcaggcg caggtcaatg cgatccgcgc
      481 cgacgtgctg agctggctcg cgccgagctt ttcttcactc cggcacggcg cgcccgacta
      541 cgcacaactg cgcagttcct gtccggcgca gatccgcgcc ggcgcgagcg acgagtcgga
      601 aatgggcgca ttccacgatc tgtttcagcc gcagcgggaa accaatttga aagtgcgcct
      661 ggaagaatat ctgcgcttcg gcctggaggc gggaagcttt tacgagacgt gaagagaaaa
      721 ttttaaccgc aaaggtaggg gcggacgtgc gtgtccaccc tttgggtcgg ggaaattaac
      781 cgcaaagagc gcaaagggcg caaaaaatat tcgtaggggc gaccggcggt cgcccgcaga
      841 tcggggatgc gaagtgcgtt ttttttcaaa gcgggacgga tgggaattaa ccacataggg
      901 cacaaagtac acaaaatgta ggggcgcgat tcatcgcgcc cgtagggtgc gcttgcgtac
      961 cattgagtaa ggacgtgtga caggatttcc gatttagcgc gaacacaggg agacactcat
     1021 gagcggtgat tacagccggg taagattcga tccgaaaaac gattttggcg ctgtgctgat
     1081 gcaacaggga cgggtgcagc tcgattccga ctggaacgag ttggcggcgc tgctcgatcg
     1141 ccgctggcgc gccgagacca ccgatatcat cggccggggc acggtgccca aggaaacgcc
     1201 ggacggcttt cgcatccagg tcgctgccgg cggcttgact atcggccgcg ggcgcatcta
     1261 cgtcgacggt tcgctggcgg agaatcacgg caaagcaccg ctggaattcg atgccgtgct
     1321 cgccgagcag cgcggcaccc tggcggtgcc gtacaatgag cagccttatt ttcccaacgt
     1381 cagcgtcgct gcgccggcgc cgaccagcgg cgggccgcat ctggtttatc tcgatgtttg
     1441 gcggcgcgag gtgacgtttc tcgaagattt gaatctggtc gaaaaagccg tcggtgtcga
     1501 tacgacgact cgatggcaga cggtctggca agtaaaaatt ctcgccaacg tcggatcgaa
     1561 cgcgacctgc ggctcgccgc tggaagcatg gaacagtttg atcgcgccgt cggccggcag
     1621 gctcagcagc gctggggtcg gcgtgccgac caacaccgat ccttgtttgc tgccgccgag
     1681 cggcggctac cgcggcctgg aaaatcgcct gtatcggatc gagattcacg acggcgggcc
     1741 gagcggcacg gcaacgttta aatggtcgcg cgacaacgca tcggtcgcga ccagcgtcac
     1801 agcgattaca gccttagaca agctcacggt tgccagcgtc ggccgcgatt cggtgatgca
     1861 tttcaagatc ggcgactgga tcgaagtcac cgacgattgg cttgagtttg cgcgccagcc
     1921 gggagtcagc ccgcaaccgg cgggagtgat ggcgcaaatt aaaaacatcg atgacgccaa
     1981 tcgcatcatc acgctcgtca gcccgattcc ggccaacgtg tttggcaccg atgcgcaggg
     2041 caacaccgat ccggcgcgcc acacgcgcgt gcgccgctgg gaccaaagcg gccaggtgcg
     2101 cgacaccaac ggcaatctgc tcgtcgatct caacgcgccg ggcgccaaag gcgtgattcc
     2161 cgtgcccgcc accggcacgt cgatcgtcct cgaagacggc gtgcagatca cgttcgacac
     2221 gccggcgggc ggcaattaca aaattgccga ctgttggaat ttcgcggcgc gcacggcgga
     2281 cgcttcggtc gaagagctga aacaggcgcc gccgctcggc gtgcaccatc acttttgccg
     2341 gctcgcgttg gtcacgtttc ccagctcggt gaccgactgc cgcacgctct ggccgccgga
     2401 ggtcgccggc gaaggctgcg agtgcacggt ctgcgtcagc gccgcatcgc acaatcaggg
     2461 caccttgacg attcaaatgg ccgtcgagca agtcaaagcc gcgggcggcg gcacgatttg
     2521 cctcggcgtc ggcacctaca atctcggcga agcgccgatg ccgatcaata tcactggcgc
     2581 gcgctcgctg actttgcgcg gccaaggcac gcagacgagt ctcatttacg caggcagagg
     2641 cacggcgctg g
//
LOCUS       DASSRL010000219         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1230569, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000219 DASSRL010000000
VERSION     DASSRL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230569"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFL49_04510"
     CDS             <1..337
                     /locus_tag="VFL49_04510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7911268.1"
                     /translation="FRNEDNAMVGGLTLANIRRGVAQAGSIGYWVGERFARKGYMTAA
                     VRALIPYSFTTLRLHRLEAACIPNNTASVRLLEKTGFKREGYARGYLCINGIWQDHLL
                     YARLKGDSD"
     gene            427..1110
                     /locus_tag="VFL49_04515"
     CDS             427..1110
                     /locus_tag="VFL49_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911269.1"
                     /translation="MVVTVNGRTATISAVAPAFLCLALALAGCGSTNVFSTSALDLFS
                     TSSKATSGDANDGVDPNADVECPGIQVRSGAATLMIGSKPGEGEPSALDLRYQATILR
                     TARECRVAAGVMTMKVGIEGRVITGPAGGPGTVDVPLRLAVVQEGVNPKTITSKFARE
                     QVTITNAVDRVTFTHIDPEISFPMPQPAANIDAYVVYVGFDPLGAQQERRKPAAKPKP
                     RVAPKPRQS"
     gene            complement(1215..1700)
                     /locus_tag="VFL49_04520"
     CDS             complement(1215..1700)
                     /locus_tag="VFL49_04520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796342.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HET7911270.1"
                     /translation="MLMEAETWVALAFVLFVGMLGYLGVHRMITKSLDDRAARIKAEL
                     DEARKLKDEAAELLAEYQRKRRAAEGEAQDIISGAKAEAERLAVEAKARIEDFVARRT
                     KMAETKIAQAEAQAAADVRSAAAEAAVAAAEKILSAEAKGKLAGELIAKGIDDVRKKL
                     N"
     gene            complement(1704..2252)
                     /locus_tag="VFL49_04525"
     CDS             complement(1704..2252)
                     /locus_tag="VFL49_04525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET7911271.1"
                     /translation="MAEPKHTTGTEHPAQGAHGGFPPFQSQTFASQLVWLAIAFVLLY
                     VLMAKLALPRVGSIIESRQKRIEGDLADASRLKGESDAAVAAYEKALADARNRAQTIA
                     NETRERQAAQAEARRRTLENELNTKLANAEKTIVATKQAAMGNVRSIAEGATRAIVER
                     LIGEVPAEKAVAAAVADVLKGS"
     gene            complement(2277..2504)
                     /locus_tag="VFL49_04530"
     CDS             complement(2277..2504)
                     /locus_tag="VFL49_04530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET7911272.1"
                     /translation="MDPVAAKYIGAGIACIGMGGAGVGVGIIFGNYLAAAMRNPSAAQ
                     GQFGNLIFGFAITEALGIFSLLIALLLLFAL"
BASE COUNT          457 a          796 c          842 g          510 t
ORIGIN      
        1 tttccgaaat gaagataatg cgatggtggg agggctgacg cttgccaata ttcgccgcgg
       61 cgtcgcacag gcaggcagca tcggctactg ggtcggcgag cgcttcgcgc gcaaaggcta
      121 tatgaccgcc gcggtgcgcg cgctgattcc gtacagcttc accaccctgc ggctgcaccg
      181 gctggaagcg gcctgcattc cgaacaacac ggcatcggtc cgcctgctcg agaaaaccgg
      241 gttcaaacgc gaaggctacg cgcgcggtta tctttgcatc aacgggatct ggcaggatca
      301 cctgctgtac gcgcggctta agggcgattc cgattgagcc ggtgccattg aagcggtgcc
      361 ttgggattgc cgatcttcac ttgctataac cgggcgggca gcattgcctc acatgtggga
      421 agagggatgg tggtgacggt aaacggacgt actgcaacta tttcggccgt cgcgccggct
      481 tttttgtgcc tggcgctggc gctggcgggc tgcggctcga ccaatgtgtt cagcacgtcc
      541 gcgctggacc tgttcagcac ctcatccaag gcaacgtccg gcgacgcgaa tgacggcgtg
      601 gacccgaatg ccgatgttga atgtccaggc atccaggtgc gcagcggtgc cgcgacattg
      661 atgatcggca gcaagccagg tgaaggcgag ccgtcggcgc tcgacctgcg ctatcaggcg
      721 acgattctcc gcacggcgcg cgagtgccgg gtcgctgccg gcgtcatgac catgaaggtc
      781 ggcatcgagg ggcgcgttat caccggtccg gctggcggac ccggtactgt cgacgtgccg
      841 ctgcgccttg cggtcgtgca ggaaggtgtc aatcccaaga ccatcacgtc gaaatttgcg
      901 cgcgagcagg tgacgattac gaacgcggtc gatcgcgtca ctttcacgca catcgatccg
      961 gagatttcgt ttccgatgcc gcagcctgcc gcgaatatcg acgcttatgt cgtgtatgtc
     1021 ggatttgatc ccttgggcgc gcagcaggaa agaagaaaac cggcggcgaa gccgaaaccg
     1081 cgcgttgcgc cgaagccgcg tcagagctga accggctcac tgaacaggca attgcacaaa
     1141 ttttcaggca aaaagaacaa acggctgcaa aaccgcatca ggcggttgaa gcctggcggt
     1201 ccgaaacggg cggcttagtt cagcttcttg cggacgtcgt cgatgccttt tgcgatcagc
     1261 tcgcccgcga gcttgccctt cgcctctgcg ctgaggattt tctccgcggc ggcgaccgcg
     1321 gcttcggctg ccgcgctgcg gacgtcggcg gcggcctgcg cttcggcttg cgcgattttg
     1381 gtttcggcca ttttggtgcg ccgcgcgaca aaatcctcga tgcgcgcctt ggcttccacg
     1441 gccagacgtt cggcctcggc ctttgcgccg gagatgatgt cctgcgcctc gccttcggcg
     1501 gcacgccgct tgcgctggta ttcggccagc agctccgccg cttcgtcctt gagcttgcgg
     1561 gcctcgtcga gttcggcctt gatacgcgcc gcgcggtcat cgagcgattt cgtgatcatc
     1621 cggtgcacgc cgagatagcc gagcatgccg acgaacagga cgaaggcaag ggcaacccag
     1681 gtttccgctt ccatcagcat ggcttatgag cccttcagca cgtcggcgac ggcggcagcg
     1741 acggccttct cggcgggaac ctcgccgatc agacgttcga caatcgcgcg cgtcgcgccc
     1801 tcggcgatgc tgcgcacatt gcccatggcc gcctgcttgg tcgccacgat cgttttttcg
     1861 gcattggcca gcttggtatt gagctcgttc tcgagggtcc tgcgacgcgc ttccgcttgc
     1921 gcggcctgcc gttcacgcgt ctcgttcgcg atcgtttgcg cgcggttgcg ggcgtcggcc
     1981 agggcttttt cgtaagccgc gacggcggca tcggattcgc ccttcagccg tgaagcatcg
     2041 gcgagatcgc cttcgatgcg tttctgccgg ctctcgatga tcgagccgac gcgcggcagc
     2101 gccagcttgg ccatgagcac gtagagcagg acaaaggcga tcgccagcca cacgagttgc
     2161 gaggcaaagg tctgcgactg gaacggcggg aatccgccgt gtgcaccctg cgcaggatgc
     2221 tcggtgcccg tggtgtgctt cggctcggcc attgtcagtc cggttcagga cgtgacttag
     2281 agcgcgaaaa gcagcaggag cgcgatcagc agcgaaaaga tgcccagcgc ttcggtaatg
     2341 gcgaacccga agatcaggtt gccgaactgg ccctgtgccg ccgacggatt gcgcatcgcc
     2401 gccgccagat agttgccgaa gatgatgccg acgccgacac ccgccccgcc catgccaatg
     2461 caggcgatgc ctgcaccgat atattttgct gcgactggat ccataacgaa ctcccttggt
     2521 tttcgagcgg gccgtcagtg gcccgggtga atcgcatcgt tgagatagat gcaggtcagg
     2581 atggcgaaga cgtaagcttg cagga
//
LOCUS       DASSRL010000220         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_5733, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000220 DASSRL010000000
VERSION     DASSRL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5733"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..294)
                     /locus_tag="VFL49_04535"
     tRNA            complement(218..294)
                     /locus_tag="VFL49_04535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Arg,seq:acg)
     gene            complement(380..880)
                     /locus_tag="VFL49_04540"
     CDS             complement(380..880)
                     /locus_tag="VFL49_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7911273.1"
                     /translation="MGEGAWLVAFVAAQRLTELCIAQLNTRRLRESGGIEFGARHYPL
                     IVVLHASWIVGLLILGHDRAVDPFWLAAFAVLQAGRLWVIGSLGRRWTTRVIVLPGAV
                     PVARGPYRWIKHPNYVIVALEIAVVPLALGLPVFALVFSAANAALLAYRIRVENKALA
                     WAAQPT"
     gene            complement(873..1922)
                     /locus_tag="VFL49_04545"
     CDS             complement(873..1922)
                     /locus_tag="VFL49_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HET7911274.1"
                     /translation="MAPPVNVLSIATAVPQHQLDQRAVADVARDVYARTFTRYPKLAE
                     VFVNAGIERRYSARPLEWLSKPHNLAERTEAYVESASELFVQTARAALDRAGIPARDV
                     DVVVTVSSTGIATPGIDARVSPELGLRPGVMRVPVFGRGCAGGVSGLALGARLARAEP
                     GEVVLVVVIELCTLTSRTDYGTKADVISSALFGDGAAAVILRAESRERALRIRAAAEH
                     TWPRTLDIMGWMIDPVGFRVMLSRSLPRFVEQRMAAPVRRFIKAARLHAPQLVCHPGG
                     AKVLDAIETALELQNGTLRDEREILRNHGNMSAPTVLFVLEHALRRGLCGPAVLSALG
                     PGFTANFLALEVFYG"
     gene            complement(1986..3362)
                     /locus_tag="VFL49_04550"
     CDS             complement(1986..3362)
                     /locus_tag="VFL49_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7911275.1"
                     /translation="MVAEVLIYVPSVANFRLNWLNDRLAAAHTAALVLDAAPSGMVPE
                     SLANQILRSIGARAVAMKMGNQRRLLAASNMPASIAQDIDMRHVTWWQAIADAFETLL
                     TCKPGDNIRVVGAAPMGGDFLEIILDEAPLCKAMWTFSVNILLLSLAISCITAMLVFL
                     ALHYLFVRPMWRITRNMVAFRADPENPSRVIAESGRRDEIGTAEEELGAMQRELSSML
                     HQQSRLAALGLAVSKINHDLRNLLASAQLFSDQLSTSPDPKVQRFAPKLMRALERAIA
                     FCQSTLSYGGAQEAPPERRTIDVEPLIEEVHEALGLGLDVPIRWIVAVERGLTMEADH
                     DQLFRVLVNIARNAVQALEARGTRDPARDQIRITGRREGSVVVIEVSDTGPGVPEKAQ
                     AHMFQAFQGSARAGGTGLGLAIAAELVRAHGGEIRLVPGTIGATFSITIPDRAVDLDT
                     RRGERARA"
BASE COUNT          721 a         1130 c         1092 g          600 t
ORIGIN      
        1 catcatcctg ctcgcagcaa tctctgcctc tctcggtgcc gccgctcgat acatttggac
       61 ggctgtacgt ggctagaaac gattgaacac gccagcctta cacaacagat cggagtcatc
      121 cgtcgcgcag tttcgattca cggaacgcgg cggattcctg aacgtggtga cggctgacgg
      181 agcgaccccg ggaggagcgc tcagttttgt tcgcgaatgg cgcgcccgaa gagattcgaa
      241 ctcctgaccc ccagattcgt agtctggtgc tctatccagc tgagctacgg gcgcttgatt
      301 ccgctgccgg aaccggcaca cgggaccggg catagctatc gtcttgatcc ggcattggca
      361 agagttgccg cgccaatgcc tatgttggct gcgcggccca ggcgagggcc ttgttttcga
      421 cgcgaattcg atacgcgagc agcgccgcat tcgcagccga gaagaccagc gcaaagaccg
      481 gcaatccaag tgccagcgga acaacggcga tctccagcgc aacgatcaca tagttcggat
      541 gtttgatcca acgatatggc ccgcgtgcga ccggcacggc gcccggcagg acgatcacgc
      601 gcgtggtcca gcgtcggcca agactgccga tgacccacag tcgtcccgcc tgcaaaacgg
      661 cgaatgccgc cagccagaat ggatcgacgg cgcgatcatg cccgagaatg agcagcccga
      721 cgatccaact cgcatgcaga acgacaatga gcgggtagtg ccgcgcgccg aattcgatgc
      781 cgcccgattc gcgcaaccgg cgcgtattga gttgcgcaat gcagagctcg gtcagccttt
      841 gcgcggccac gaatgcgaca agccacgcac cctcacccat agaacacctc gagcgccagg
      901 aaattggcgg tgaagccggg acccagcgcg gaaagcacgg ccggtccgca cagaccacgt
      961 cgcaaagcat gttcgagaac gaagagaacg gtcggcgccg acatgtttcc atggttgcgg
     1021 agaatctccc gctcatcacg cagcgtgcca ttctgcaact cgagggccgt ttcgatggcg
     1081 tcgaggacct ttgcgccacc gggatgacag acgagttgcg gcgcatgcag acgggccgcc
     1141 ttgatgaaac ggcgcaccgg tgccgccatt ctttgttcga caaaccgcgg cagcgagcgc
     1201 gagagcatca cccgaaaacc aaccgggtcg atcatccagc ccatgatgtc gagcgtgcgt
     1261 ggccatgtgt gctcggccgc ggcacgaatg cgcaacgccc gctcacggga ctcggcccgc
     1321 agaatcaccg cggcggctcc atcgccgaac aatgccgacg aaatcacgtc agctttggtc
     1381 ccgtagtcgg tacgagaggt caatgtgcac agctcgatga caaccacgag cacgacttcg
     1441 ccgggttcag cccgtgcaag ccgcgcgccg agcgcaaggc ccgaaacgcc gccggcacaa
     1501 ccacggccaa ataccggcac gcgcatcacg cccgggcgta atccaagttc ggggctgacg
     1561 cgcgcatcaa ttcccggcgt agcgatgcct gtcgaagaca ctgtcacgac gacatcgaca
     1621 tcgcgcgccg ggattccggc acggtcgagc gcggctctcg ccgtttgaac gaaaagctcg
     1681 cttgcacttt cgacgtaagc ttccgtacgt tcagccagat tgtgcggctt gctcagccat
     1741 tcgagcggcc gcgcggaata gcggcgctcg atgccggcgt tgacgaatac ctccgcaagc
     1801 ttcggatacc gcgtgaaagt gcgtgcgtag acatcgcgcg cgacatctgc aaccgcgcgc
     1861 tgatcgagtt gatgctgcgg caccgcggtc gcgatcgaca gcacgttgac gggcggcgcc
     1921 atgccggatg tccttcagtc gcacaagccc ttgaggaaga acaccgccgg cggcgtctgc
     1981 ttccgtcagg cgcgagcgcg ctcaccgcgc cgggtgtcga gatcgacggc ccggtcgggg
     2041 atggtgatgc tgaaggtggc gccgattgtg ccgggcacga ggcggatttc gccgccatgg
     2101 gcacgcacaa gctccgcagc aatggcaagc cccaggccgg taccaccggc ccgcgcggag
     2161 ccctggaaag cctggaacat gtgagcttgc gccttttccg gcacgccagg gccggtatcg
     2221 gaaacttcaa tcacgaccac gctgccttca cgccgtccgg tgatacgaat ctgatcgcgc
     2281 gccggatcgc gcgtaccacg cgcttccagc gcctgcaccg cattacgcgc gatattgacg
     2341 agaacgcgaa acaattgatc gtgatcggct tccattgtca ggccacgttc gacggcaacg
     2401 atccagcgga tcggcacatc gaggccgagg ccaagcgctt cgtgcacctc ttcgatcaac
     2461 ggctcgacgt caatcgtccg acgttcgggg ggcgcctcct gcgcgccgcc gtaagacagg
     2521 gtgctttgac agaacgcaat cgcgcgttcg agagcgcgca tcagcttggg cgcgaagcgc
     2581 tgcactttcg gatcgggcga ggtggacagc tggtcggaaa agagctgggc cgacgcaagc
     2641 aggttgcgaa ggtcgtgatt gatcttcgat accgcaaggc cgagcgccgc gagtcgactc
     2701 tgctgatgca gcatcgagga cagttcgcgc tgcatggcac cgagttcttc ctcggctgtg
     2761 ccgatttcgt cgcgacggcc actttcggcg atcacccggg aagggttctc gggatcggcc
     2821 cggaacgcaa ccatgttgcg cgtgatgcgc cacatcgggc gcacgaacag atagtgaagc
     2881 gcaaggaaca caagcatcgc cgtgatgcag gagatcgcca gcgacaaaag caggatgttc
     2941 accgagaacg tccacatcgc cttgcacagt ggtgcttcgt cgagaatgat ctcgaggaag
     3001 tctccgccca tcggcgcagc gccgacaacg cggatgttgt cgccgggctt gcaggtaagc
     3061 agggtttcga acgcatcggc aatcgcctgc caccaggtca cgtgccgcat gtcgatgtcc
     3121 tgggcgatgc tggccggcat attggatgca gccagcaggc gccgttgatt gcccattttc
     3181 atcgctaccg cgcgcgcacc gatgctgcgc agaatctggt tggcgagact ttccggcacc
     3241 atgccgctcg gcgccgcatc aaggaccagc gccgccgtgt gagcggcggc caacctgtcg
     3301 ttcagccagt tgaggcggaa gtttgcgacc gaaggcacgt aaatcagaac ttcggccacc
     3361 atcacgaaca cgatcgtcag caaaagcagt ttgccggaga gtccgatgcg cggctttcgc
     3421 cccgccggcc ccgtttcgat cgcggtttcc ggtttgtcga tcgattccaa ttttgcctca
     3481 gccgaagatt ctcagccaac cgatgatgcg acgcacccac acgccggtca aacttggggt
     3541 gaa
//
LOCUS       DASSRL010000221         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1160667, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000221 DASSRL010000000
VERSION     DASSRL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160667"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1110
                     /locus_tag="VFL49_04555"
     CDS             205..1110
                     /locus_tag="VFL49_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7911276.1"
                     /translation="MKAIFLDCNDQLAPVWERVVRPPDDPPIDVNRRPFARDELPRII
                     GGYDIAIDDHSYMPTPLVAQCPELKHIVFLGTGPASYMNVGELNARGIKVHIIKGYGD
                     TAVAEHTVALMWACARDIARMDREVRRGVWTPHEGVQLLGKTLGVIGLGGIGREVARI
                     GKGTGMNVIGWNRTKRPEVEVPIVDIDTLLARADIVSMNLTLGDETRGFLSAERIARM
                     KKGAIYVNTARGALADEAALIAALQSGHIRHAGLDVFHNEPLSPDHALAKLENVTLSA
                     HAAFRTPEASETLLRRAIDIVKSIS"
     gene            1175..2017
                     /locus_tag="VFL49_04560"
     CDS             1175..2017
                     /locus_tag="VFL49_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7911277.1"
                     /translation="MKPAPFAYTRARTLKEAVALLAKHKDDARLLAGGQSLIATLNMR
                     LSAPDLLIDINGIKNLNGIAKKGKHVEIGALARHAQVERSDVVAKYAPLIALAIPHIG
                     HPAIRNRGTFGGSIAFADPAAELPACLLALGGEIDIAGPRGKRRIKADVFFKGLFETA
                     LRAQEMITAIRVPIADKNARVGFAELARRHGDYAMVGLAASARSSGKGLADVRLAYFG
                     VGDTPVRAKKAEAALASGDIDAAVKALDLDPHDDVQATAKTKKHLAGVLLRRVAKQLM
                     EPRG"
     gene            2014..2502
                     /locus_tag="VFL49_04565"
     CDS             2014..2502
                     /locus_tag="VFL49_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7911278.1"
                     /translation="MNNHAVEISLTVNGERVRERIDARKTLVDFLRDDLSLTGSHVGC
                     EHGVCGACTVRVDGEIVRGCLMLAVQCDGAKVETIEGLSDSGEVADLQTAFEQRNALQ
                     CGFCTPGMLAAAQDLLKRAKKMPSRDEIREHLSGNYCRCTGYHAIVDAVEAVAQARAK
                     GR"
BASE COUNT          567 a          791 c          821 g          481 t
ORIGIN      
        1 gtgctgcttt atccaatagt tgtgaatcag cggcgaacgg gaatgaccgg cgggccaggc
       61 gataacacag acagctttgt gttctttact cattactccg ctacattatg ttgcgtcagt
      121 gatcggtgca tgtaatccgc accgcgtgac ccgtcaacgc ttgcaaattt aattcctgtt
      181 cagtaacgcc cgaacgagat tcccatgaaa gctattttcc ttgactgcaa cgatcagctt
      241 gctcccgttt gggagcgtgt tgtccggccg ccggacgatc cccctatcga cgtgaaccgc
      301 aggccgtttg cacgcgatga gctgccgcgc atcatcggcg gctatgatat tgcgatcgac
      361 gaccattctt atatgccgac gccgcttgtc gcccaatgtc cggaactcaa gcacattgtt
      421 tttttaggca ccgggccggc gagctacatg aacgtcggcg aactcaatgc gcgcggcatc
      481 aaagtgcaca tcatcaaggg ttacggtgat accgcggtcg ccgagcacac cgtcgccttg
      541 atgtgggctt gcgcccgcga tattgcgcga atggaccgcg aggtgcgccg cggcgtatgg
      601 acgccgcacg aaggcgtcca actgctcggc aaaacgctgg gagtgattgg gctcggtggc
      661 atcggccgcg aagtggcgcg tatcggcaaa ggcacgggca tgaatgtgat cggctggaat
      721 cggacgaaac gcccggaggt cgaagtgcct atcgtggaca tcgacacgct gctcgcgcgc
      781 gcagacatcg tatccatgaa cctgacgctc ggcgacgaga cgcgcggatt tctctccgcc
      841 gagcggattg cgcgcatgaa gaaaggcgca atctacgtca ataccgcccg cggtgccctc
      901 gccgacgagg ccgcattaat tgcggcgctt caaagcggac atattcgcca tgcaggtctc
      961 gacgtcttcc acaatgagcc gctgtcaccg gaccatgccc tcgcaaagct cgagaacgtg
     1021 acattgtcgg cgcatgcggc attccgtacc cccgaggcat ctgaaacatt gctgcggcgc
     1081 gcgatcgaca tcgtgaagtc catttcctag ccacttgcgg ccacattgac ctgtccgcgt
     1141 tcccggtcac aataagagaa acggggaagg aaacgtgaag ccggcgccat tcgcatacac
     1201 cagggcgagg acgctcaagg aagccgtggc gttgctcgca aagcacaagg acgacgcgcg
     1261 gctgcttgcc ggcgggcaga gcctgattgc tacgctcaat atgcggctgt cggcgccgga
     1321 cctgctgatc gacattaacg gcatcaagaa cctcaacggc atcgccaaaa agggcaagca
     1381 cgtcgagatc ggcgcgctgg cgcgccacgc gcaggttgag cggtctgacg tcgtcgcaaa
     1441 atacgccccg ctgatcgcac tcgcgatacc ccatattggc catccggcaa tccgcaaccg
     1501 gggcaccttc ggcggctcta tcgcttttgc cgatccggca gccgagctgc ctgcctgcct
     1561 gctggcgctc ggcggggaaa tcgacatcgc aggcccgcgc ggaaaacgca gaataaaggc
     1621 tgacgttttc ttcaagggtc tgttcgaaac cgcgctgcgt gcccaggaaa tgattaccgc
     1681 gatacgcgtt cccattgccg acaaaaatgc gcgcgtcggc ttcgccgagc tggcgcgccg
     1741 gcatggcgac tacgccatgg tcggcctggc ggcgagcgcg cgcagcagcg gaaaggggct
     1801 ggccgatgtg cggcttgcct atttcggcgt tggcgatacc ccggtccgtg cgaaaaaagc
     1861 cgaggcggcg ctcgccagcg gtgatatcga cgcagccgtc aaagcgctcg atctcgatcc
     1921 gcatgacgac gtgcaagcga cggcaaagac gaagaagcat ctcgcggggg tcctgctccg
     1981 ccgtgttgca aagcaactga tggaaccgcg cggatgaaca accatgctgt cgaaatctcg
     2041 ctgaccgtca acggcgagcg cgtgcgggag cggatcgatg cgcgcaaaac gctggtcgac
     2101 ttcctgcgcg atgatctttc tctgaccgga agccatgtcg gctgcgagca cggcgtttgc
     2161 ggggcctgta ccgtgcgcgt cgacggagaa atcgtgcgcg gttgcctgat gctggcagtg
     2221 cagtgcgacg gcgccaaagt ggaaactatc gaaggcctgt ctgattcagg cgaagttgcc
     2281 gatttgcaga cagccttcga gcagcgcaat gcgctacaat gcggcttctg cacgcccggc
     2341 atgctggctg cggcccagga tttgctgaaa cgcgcaaaga aaatgccgag ccgcgacgaa
     2401 attcgcgagc atctgtcggg caactactgc cgctgcaccg gctaccacgc gatcgtcgat
     2461 gctgttgagg cggtggccca ggcgcgggcg aaaggaaggt aggccatgaa cgagcatgcg
     2521 catcggctgt cgatgtcgga acgggccgac gagcttgaag aagaaaaaat gcacgaaatg
     2581 gaacgcatca tcaccaagtc gaccatcgcg cacctgatgg acggcgaggt gaaaatggat
     2641 accggccccg aaatgaaggc
//
LOCUS       DASSRL010000222         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_705620, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000222 DASSRL010000000
VERSION     DASSRL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705620"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="VFL49_04570"
     CDS             <1..1898
                     /locus_tag="VFL49_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7911279.1"
                     /translation="VGLWLAIGGAGFVAWVGAHLPPIQSLEIPKRPPSIHIVDFQGRA
                     MARRGDLAGAPLPLKDLPAYVPRAFIAIEDRRFYDHSGVDPYGIGRALIANVLHRGVA
                     QGGSTITQQLAKNLFLTQERTITRKLQEALLAIWLERKFSKTQILELYLNRVYFGSGA
                     YGIEQASQHYFGKSARQITVAEAALLAGVVKSPSRLAPTRNFDGAEKRARAVLVAMAE
                     LKFISHSTERTALAHPPRIVAQANTGSVNYVADWVMDALNDTLGRVEEDITVQTTIDA
                     NLQANAEKALTDELAQKGDKNGVGQGALVAMTPDGAVRALVGGRNYAESQFNRAVSAK
                     RQPGSAFKPFIYLTAIEHGLTPDSVREDRPINVKGWQPENYGHEYFGPVTLTKALALS
                     LNTVSVRLTLEFTPLAVIRTAYRLGIVSKLEPNASIALGTSEVSMLELVGAYATFANG
                     GYALVPHVVQKITAANGKVLYARNTGQLGRIVEARYVAMMNQMMAETLTIGTAHKAAL
                     PGWPAAGKTGTSQDFRDAWFIGYTAHLVTGVWLGNDDGTPTKHVTGGGLPVEIWSRFM
                     RTAHQGVPVASLPGASGGGLFTGLFGNNSRPIPPSPVQPAAGTRASTTGTGGLDSWLL
                     NGLFGRR"
     gene            complement(1895..>2850)
                     /locus_tag="VFL49_04575"
     CDS             complement(1895..>2850)
                     /locus_tag="VFL49_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7911280.1"
                     /translation="YCAASLACALSTSIEMLIVARAFQALGGCGGIVLTRAIVRDIYS
                     GAHAGRELSVIGSVMALAPVLAPIVGGIIQTAFGWRVTFLALVGAGLAGGAIVWSLLP
                     ETLTVRAAERVSIASMLRSYRLVARNPAYLAYLAITSASYAGLFAWISGSAFVLQDLY
                     GLAPFDFGVAFAVGSVGYMGGSAIAARVVVRLGLDGVLGLGACACAAGGLAMTAAVAL
                     GFVSSMALVLPMAVYLAGLGLVLPQGIAGAMTPFPERAGAASSLFGFVQQTAAAICGA
                     AVGWFLGQSAWPLALGVASMGLATLGFWLATRGLRARAAKH"
BASE COUNT          574 a          977 c          851 g          448 t
ORIGIN      
        1 tcgtcggcct ttggcttgcg atcggcggcg ccggattcgt ggcctgggtc ggcgcgcatc
       61 ttccgccgat ccagtcgctg gaaattccca aacgcccgcc gtcgattcac atcgtcgact
      121 tccagggccg cgcgatggcc cgccgcggcg accttgccgg tgccccgctg cctttgaagg
      181 acctgccggc ttatgtcccc agggctttta tcgctatcga ggatcggcgc ttctacgatc
      241 acagcggcgt tgacccctat ggtatcggtc gcgccctgat cgccaacgtc cttcaccgtg
      301 gcgttgccca gggcggatcg acgatcacgc aacagctcgc aaaaaatctt ttcctcacgc
      361 aggagcgcac gatcacgcgc aagctgcagg aggcgctgct ggcgatctgg ctcgagcgca
      421 aattctcgaa gacccagatt ctcgagctct atctcaaccg cgtctacttt ggctcgggcg
      481 cctacggcat cgagcaggct tcacagcact acttcggaaa gtccgcacgg cagatcaccg
      541 tcgcggaagc tgccctgctc gcaggcgtcg tgaaatcccc gtcgcgtctt gcacccacgc
      601 gcaacttcga tggcgccgaa aagcgcgcaa gggccgtgct cgtggccatg gcggagctca
      661 aatttatttc gcattcgacg gagcgtaccg ccctggcgca tccgccgcgt atcgtggcgc
      721 aggcaaacac cggctcggtc aattatgtcg ccgactgggt catggacgcg cttaacgata
      781 cgctcggccg cgtcgaagaa gacatcacgg tgcagaccac catcgacgca aatctccagg
      841 ccaatgcgga aaaagcgctg accgacgagc ttgcacagaa aggcgacaag aacggggtcg
      901 gacagggcgc gctggtggcg atgacgcccg acggcgccgt tcgagctttg gtcggcggcc
      961 gcaactacgc cgaaagccag ttcaatcgcg cggtctcggc aaaacgccag cctggttccg
     1021 ccttcaagcc gttcatctac ctcaccgcga tagagcacgg gctgacgccc gacagcgtgc
     1081 gtgaggaccg tccgatcaac gtcaaaggct ggcagccgga gaactacggc cacgaatatt
     1141 tcggacccgt gaccctgacg aaggccctcg ccctttcact caacaccgtt tcggtacgcc
     1201 tgacgctgga attcactccg ttggcggtga tccgcaccgc ctaccggctc ggaatcgtgt
     1261 cgaagctcga gcccaatgcc tcgatcgcgc tcggcacctc cgaagtgtcg atgctggagc
     1321 ttgtcggcgc ctatgcgaca ttcgccaatg gcggctacgc gcttgtcccg cacgttgtcc
     1381 agaagatcac cgccgcgaac gggaaagtgc tctacgcgcg caataccggc cagctcggcc
     1441 gcatcgtcga ggcgcgttac gtggccatga tgaaccagat gatggccgag acacttacca
     1501 tcggcaccgc gcacaaggca gcgctgcccg gctggcccgc cgcgggaaaa accgggacgt
     1561 cgcaggattt ccgcgacgcc tggttcatcg gttacaccgc gcatctcgtg accggcgtct
     1621 ggctcggcaa tgacgatggc acgccgacaa agcatgtgac cggcggcggc ttgccggtcg
     1681 agatctggag ccggttcatg cgcactgccc atcagggcgt gccggtcgca agcctgcccg
     1741 gtgcgtccgg cggcggattg ttcacgggat tgttcggcaa caattcgcgg ccgataccgc
     1801 cctcgccggt gcaaccggct gctggcacgc gcgcgtccac gacaggaacc ggcggtctcg
     1861 acagctggtt actcaacggc ctcttcggcc gccgctaatg ctttgctgcg cgggcgcgga
     1921 gcccgcgggt cgccagccaa aaccccaatg tcgccaagcc catactggcg acaccgagag
     1981 cgagcggcca ggcgctttgc ccaagaaacc atccgaccgc ggcgccgcag atcgctgccg
     2041 cagtttgctg cacgaatccg aacagcgatg acgcagcgcc ggctctttcg gggaacggcg
     2101 tcattgcgcc ggcaatcccc tgcggcagca ccaggccgag gcccgcaaga tagacggcca
     2161 tcggaagcac cagcgccatg gatgacacaa atccgagcgc gacagcagcg gtcatcgcaa
     2221 gccctccggc ggcgcaagca caagccccca atccaagcac cccatccagg ccaaggcgaa
     2281 ctacaacccg cgctgcaatc gccgaccctc ccatgtatcc aaccgaaccg accgcgaagg
     2341 ccacaccgaa atcgaacggc gcaagtccat aaagatcctg cagcacgaat gccgatccgg
     2401 agatccaggc aaatagcccg gcatagctcg ccgacgtgat cgcgagatag gcgagataag
     2461 ccggattacg tgcaaccagg cggtaggatc gcagcattga tgcaatcgaa acccgctccg
     2521 ccgcgcgcac cgtcagcgtt tcaggcagaa gcgaccagac gatcgctccg cctgccagtc
     2581 cggcgccgac caatgccagg aatgtcacgc gccagccgaa tgcggtctgt atgatgccgc
     2641 cgacaatcgg cgccagcacc ggcgcaagcg ccatgaccga gccgatcacc gacaattcgc
     2701 gcccggcgtg cgcgcccgaa tagatatcgc gcacgatcgc gcgcgtaagc acgatgcccc
     2761 cgcagccgcc gagtgcctga aatgcacgcg cgacaatgag catctcgatc gaggtggaca
     2821 gcgcgcaggc aaggcttgcc gcgcagtaga
//
LOCUS       DASSRL010000223         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1073267, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000223 DASSRL010000000
VERSION     DASSRL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073267"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFL49_04580"
     CDS             <1..313
                     /locus_tag="VFL49_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7911281.1"
                     /translation="ADNGIYGTIRMHQEREYPGRVVATDLRNPDFVAYALAFGGYGAV
                     VEKSADFPAVFAAARASGKPSIIHLKIDPEAITPATTLSAIRESGIRSRESGRSPASN
                     S"
     gene            415..1113
                     /locus_tag="VFL49_04585"
     CDS             415..1113
                     /locus_tag="VFL49_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7911282.1"
                     /translation="MPYRRTENVVRRLAAREQTILDAAQSIASESGMGAVQIAPVAHR
                     ADIAAGTVYRYFPSKNNLIAEVIASAAGRELAAMREAADAAPGPLSALAACIATFAAR
                     ALNDRRLTWAVLAEPVDANIDTLRVEFRRAIAGELAWRISVAISGRHLPDQDARAAAP
                     AIVGALLEGLIGPLASPGGNAREAVQAITLLSLRALGVVDARARGLVAQCPLPQNPSA
                     TSAPAPSLIQAAPA"
     gene            complement(1061..1888)
                     /locus_tag="VFL49_04590"
     CDS             complement(1061..1888)
                     /locus_tag="VFL49_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7911283.1"
                     /translation="MIKDIVVTLGLGAQDPAGAFAISVAEKFEAHLVGIAVSYEPVIP
                     GTVMGGVPPEVIEGQRAESTKRANAAIARFEQAAKRAGISMETRLISASVAGAADQIG
                     RIARRFDLAVVGQPARKPSLPDQVVDEGVLFESGRPVIFVPFVQKAGIKLERIMVCWD
                     GSRTASRAVADAMPLLKKAKQVEIVIVSSKPARDDELPGADLGQHLARHGLNIGIKRI
                     TSPDTDVPSTILSYAADSSADMIVMGGYGHSRLREFVLGGVTRGLLESMTVPVLMSH"
     gene            complement(1972..2355)
                     /locus_tag="VFL49_04595"
     CDS             complement(1972..2355)
                     /locus_tag="VFL49_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HET7911284.1"
                     /translation="MKSIFAAAMLMTAMIQVAHAQDVAAGEQSFKKCFPCHSVGEGAK
                     NKVGPELNGLDGRHSGSAPDYNYTDANKNSGITWNETTFKEYIKDPRAKIPNTKMIFP
                     GIKNEKEAGDLWAYLKQFDATGKKK"
     gene            2503..>3945
                     /locus_tag="VFL49_04600"
     CDS             2503..>3945
                     /locus_tag="VFL49_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7911285.1"
                     /translation="MSVRMPEADAEILGRRDRIVAALRSIVPGEGVIASAREMRAYET
                     DGLTAYRQLPMVVALPETTQQVAEVLRYCHDEGIKVVPRGAGTSLSGGALPLGDGVLL
                     GMAKFNRIREIDFDNRVAVVEPGVTNLAVSNAVAHAGFYYAPDPSSQIACTIGGNVAE
                     NSGGVHCLKYGMTTNNLLGCEIVLINGEVLRIGGKHLDAAGYDLLGLITGSEGLLGVV
                     TEVTVRILKKPETARALLVGFQSSEDAGECVSRIIGAGIIPGGMEIMDGPAIAAAEQF
                     VHAGYPLNVEALLIVELDGPSAEVDHLIERVEKIARDCRSVSCKASGSEEERLLFWAG
                     RKAAFPAVGRISPDYYCMDGTIPRAQLPLVLRRMKEMSAKYGLRVANVFHAGDGNLHP
                     LILYDANVPGELEKTEQFGAEILKLCVEVGGVLTGEHGVGVEKRDLMPTMFSEIDLNQ
                     QQRLKCAFDAEGLLNPGKVFPTLHRCAELGR"
BASE COUNT          697 a         1220 c         1241 g          787 t
ORIGIN      
        1 cgccgataac ggcatctacg gcaccattcg catgcaccag gagcgcgagt atccgggccg
       61 cgtcgttgcg accgacctgc gcaacccgga ttttgtggct tatgcgctgg cgttcggcgg
      121 ctatggcgcg gtggtggaga agagtgcgga ttttccggca gttttcgccg ccgcccgtgc
      181 ttccggcaaa ccgtcgatca tccatctcaa gatcgacccg gaggcaatca cgccggccac
      241 gacactgtcc gccattcggg agtcggggat caggagtcgg gaatcaggaa gatccccagc
      301 gtctaactcc tgactcctgg ttcccgaccc ctgaccttct gacttctgac ctcctgatcc
      361 ctgatccctg attcccgctt gccatgcgga cgtgaatcag cgttcagttg atccatgccc
      421 tatcgccgga ccgaaaacgt cgtccgtcga cttgccgcgc gcgagcagac gatccttgat
      481 gccgcgcaat cgattgcgtc ggaaagcgga atgggcgcgg tgcagattgc gccggtcgcc
      541 caccgggccg atatcgccgc cggcacggtt tatcgctatt tcccgtccaa aaataacctg
      601 attgccgagg tgatcgcgtc ggctgccgga cgtgagctcg ccgccatgcg ggaggctgcc
      661 gatgcggcgc cgggaccgct ttccgcgctg gccgcctgca ttgccacctt cgccgcgcgt
      721 gccttgaatg accggcggtt gacctgggcc gtgctggcgg agccggtcga cgccaatatt
      781 gatacattgc gggtggaatt ccgtcgcgcg attgcaggcg agttggcatg gcggatcagt
      841 gtcgcaatca gtggccggca tctgcccgat caggacgcgc gtgccgccgc gcccgcgatc
      901 gtcggtgcgt tgcttgaagg tctgattggc cccctggcgt cgccgggcgg caatgcgcgc
      961 gaggccgtac aggcgatcac gctgctgtca ctgcgcgcgc ttggggtggt tgatgcccgc
     1021 gccagagggc ttgtcgctca gtgcccgttg ccgcaaaacc ctagtgcgac atcagcaccg
     1081 gcaccgtcat tgattcaagc agcccccgcg taacgccgcc gagcacgaat tcgcgcaggc
     1141 gtgaatggcc atagccgccc atcacgatca tgtcggccga ggaatcggcg gcgtaggaca
     1201 agatcgtcga gggcacatcg gtgtcgggcg aggtgatacg cttgatgcca atattgaggc
     1261 cgtgccgggc gaggtgctgg ccgagatcgg cgccgggcag ttcgtcgtcc cgggccggtt
     1321 tacttgaaac gatgacgatc tcgacctgct ttgccttctt caggaggggc atggcatcgg
     1381 cgaccgcgcg actggccgtc cggctgccgt cccagcacac catgatgcgt tcaagtttga
     1441 tgccggcctt ctggacaaat ggcacgaaga tcaccgggcg gccggattcg aacaggacgc
     1501 cctcatcgac gacctggtcg ggcaaagagg gcttgcgcgc cggctgtccg acaacggcga
     1561 ggtcaaaacg tcgggcaatc ctgccgatct ggtcggcggc gcccgcgaca cttgccgaga
     1621 tgagccgggt ttccattgag atgcccgcgc gcttcgccgc ctgctcgaag cgtgcgatgg
     1681 ccgcgttcgc ccgcttggtc gattcggccc gctgaccttc gatcacctcg ggaggaaccc
     1741 cacccatcac cgtgcccgga atgaccggct cataggagac ggcaattcca accaggtgcg
     1801 cctcaaattt ctccgcgacc gaaattgcga acgctcccgc gggatcctgc gcgcccaggc
     1861 cgagagtgac gacgatatct ttgatcatga acggaccttc ctgatctggt aacgcccgac
     1921 cgagtcatgc ctccgcgccc cggcttgcct cacgcggttc tctgcccaat tctatttctt
     1981 cttgccggtg gcgtcgaatt gcttgagata ggcccaaaga tctccggctt ctttctcatt
     2041 cttgatgccc gggaaaatca tcttggtgtt cggaatcttg gcacgtggat ccttgatgta
     2101 ttccttaaag gtcgtctcat tccaggtaat gccggaattc ttgttggcgt cggtgtagtt
     2161 gtagtcggga gccgagccgg aatggcgccc gtccagaccg ttcagctcgg gtccgacctt
     2221 gttctttgcg ccctcgccga cagagtggca cggaaagcac ttcttgaacg attgctcgcc
     2281 tgcggcgaca tcctgtgcgt gcgcaacctg aatcattgcg gtcatcaaca tggccgcggc
     2341 aaatatcgac ttcatctctc ttgctccagt ttgtgatgtt ggctgccgcc cgcgacgaaa
     2401 aacaccttga ggctaccccg gtttccattg gtttttgcca gccctatgtg taagccgtca
     2461 aatggctgat atcgttgttt ggacataacg gagttgcgcg aaatgtcggt gcgtatgccc
     2521 gaagccgatg ccgaaatcct gggccgccgg gaccgcatcg tggcggcttt gcgcagcatc
     2581 gttcccggcg agggggtcat cgccagcgcg cgcgagatgc gtgcctatga aaccgacggc
     2641 ctgacggcct atcggcagct acccatggtc gtggcgctcc cggagaccac gcagcaagtc
     2701 gccgaggtgc tccggtattg tcacgatgag ggcatcaagg tggtcccacg cggggccggc
     2761 acgtcactgt ccggcggcgc gcttccgctc ggcgacggcg tgctgctcgg tatggcgaaa
     2821 ttcaaccgga tccgggaaat cgactttgac aatcgcgtcg ctgtggtcga gccgggcgtc
     2881 accaacctgg cggtgtctaa cgccgttgcc catgccggct tttactacgc gccggacccg
     2941 tcctcgcaga tcgcgtgcac gatcggcggc aacgtggccg agaattcagg cggcgtgcat
     3001 tgcctcaaat acggcatgac gaccaacaac ctgctgggat gcgagatcgt gctcataaac
     3061 ggcgaggtgc tgcgcatcgg gggcaagcat ctcgacgcgg cgggctatga ccttctcgga
     3121 ttgatcaccg gctcggaagg cttgctgggc gtggtaacgg aagtgacggt ccgcatcctg
     3181 aaaaagccgg agacggcgcg cgcactgctc gtaggcttcc aatcgtccga ggatgccggc
     3241 gaatgtgtgt cgcgcatcat cggggcggga atcatacccg gcggcatgga aatcatggat
     3301 gggccggcca ttgcggcggc cgagcaattc gtgcacgccg gttacccgct caacgtcgaa
     3361 gcgctcctga tcgttgaact cgacgggcct tccgccgagg tcgatcatct gatcgagcgt
     3421 gtggagaaaa tagcgcgcga ttgccggtcg gtctcctgca aggcgtcggg ttcggaagag
     3481 gagcggctgc tgttctgggc cggccgcaag gcggcattcc cggcggttgg acgcatctcg
     3541 cctgactact attgcatgga cggcaccatt ccgcgtgcac agttgccgct ggtgctcagg
     3601 cgcatgaaag agatgtcggc aaaatacggc ctgcgggttg ccaatgtttt tcatgccggc
     3661 gacggcaacc tgcaccctct cattctttac gacgccaatg ttccgggcga gctggagaaa
     3721 acggaacaat tcggcgccga gattctcaag ctatgcgtcg aggtcggcgg cgtgctcacc
     3781 ggcgagcatg gagtcggcgt ggaaaagcgt gacctgatgc cgacaatgtt ctcggagatc
     3841 gacctcaacc agcagcaacg gctgaaatgc gccttcgacg cagagggcct gctcaacccc
     3901 ggcaaggtgt ttccgacgct tcaccgttgc gccgaactcg gccgc
//
LOCUS       DASSRL010000224         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1370785, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000224 DASSRL010000000
VERSION     DASSRL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370785"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /locus_tag="VFL49_04605"
     CDS             complement(<1..2032)
                     /locus_tag="VFL49_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911286.1"
                     /translation="MANIPQKTKDATEEALTAIQEALNIRPPDTRAGPSFGPAEAGLP
                     DPQSAHEPGDLFPQDGPQSGWPSGEATPRRAANDDRAGIGQILQTLRHRSSRGPYIAA
                     GIAGLVWAAVSVAALYAIDTQAVFGTAKLGFAALLAIGGAIVVPIVFFFVLAHMFNRA
                     QDMRVVAESMAEVAMRMAQPETVARESIVTVGQAIRREVAAMGDGVERALARAAELEA
                     LVHNEVSALERAYNDNEVRIRDLLTELSNQRETLVNQAEQVRNAIASVHLDLAHDITS
                     VGDLVADKVNEVAQRVTRSLTEKGEHITLALGHAGDSMIDALSERGSTLLDQLETTSD
                     QATTAISSASDRLSASLNFKTEHVHDEFAELAARLQQMMTARLDQVAQGFADRSTVTI
                     NSMASRSQQLTEAIAETSNRLAETIGARAEEVNTALRSTGDSLILDLSLRGGDVVSKL
                     EQTGARIADTMSSRSDAVTESFRENAETLVAALNDRGEAVKEMLASRLQDFEAMFNQG
                     GTEITEKISRDASNLGNLITRHITEFDHTVKNYGGELVGRLGQRTQDVAEALRNYVDT
                     FDQRVSGRTGEISTTLDQRLAQFETLLAARVSDLAKTLTEGGKDVVTALDQRVGEVAG
                     TINSRGIEVADSISARIGEMDRMLGARALEVADNLDSRIGRFEELLVGRAETV"
     gene            2451..2831
                     /locus_tag="VFL49_04610"
     CDS             2451..2831
                     /locus_tag="VFL49_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET7911287.1"
                     /translation="MAQAAVQISERVQADAPQVAVLDRAHLARMTFGDRSLELEVLQL
                     FDRQAELLLDRMRGSQPAAIVTLAHTLKGSAVGIGATQVARAAAETEAIAHAKPGECN
                     RAIAQLAQAVKEARAEISALIRAH"
     gene            2911..>3145
                     /locus_tag="VFL49_04615"
     CDS             2911..>3145
                     /locus_tag="VFL49_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7911288.1"
                     /translation="MPKITFTDSSGAARTVEAENGSTVMETAIRNNIPGIEAECGGAC
                     ACATCHVYVDEAWREKVGGPSPMEEDMLDFGYDV"
BASE COUNT          571 a          959 c         1004 g          611 t
ORIGIN      
        1 tgacggtttc tgcccggccg acaagcagct cttcgaacct tccgatgcgg ctgtcgagat
       61 tgtccgcgac ctcgagcgcc cgcgcgccga gcattcgatc catttcaccg atcctggcgc
      121 tgatcgaatc ggccacttcg atgccgcgcg aattgatcgt ccccgccact tcgccgactc
      181 gctggtcgag tgcggtgacg acgtccttgc cgccctcggt gagggtcttt gcaagatccg
      241 agacacgcgc cgcgaggagc gtctcgaact gcgcgagacg ctggtccagc gtggtcgata
      301 tctcgcccgt ccggccggac acgcgctgat cgaaggtatc gacgtaattg cgcagagcct
      361 cggccacgtc ttgcgtgcgc tggccgagac ggccaaccag ttcgccaccg tagttcttca
      421 ccgtatggtc gaactctgtg atgtgccggg tgatcaggtt gccaaggttg cttgcatcgc
      481 gggagatctt ctcggtgatc tcggtgccgc cctgattaaa catcgcttcg aagtcctgca
      541 gccgcgacgc gagcatttcc ttcactgcct cgccgcggtc gttgagagcg gcgaccagcg
      601 tttcggcgtt ctcgcggaaa ctttcggtaa ccgcgtcgct gcggctggac atggtgtcgg
      661 ctatgcgcgc gccggtctgc tcaagtttcg agaccacgtc gccaccgcgc agcgacaggt
      721 caagaatgag cgagtcgccg gtcgagcgca gcgccgtgtt gacctcttcg gcgcgtgcac
      781 caatcgtctc cgcaagacga ttgctggttt cagcgatggc ctcggtcagc tgctgggagc
      841 ggctagccat ggagttgatg gtgaccgtcg atctgtcggc aaaaccctgc gcgacctggt
      901 caagtcgcgc ggtcatcatc tgctgcagac gggcggcgag ttcagcgaac tcgtcgtgca
      961 catgctcggt cttgaaattg aggctggcgg acagacgatc gcttgccgat gagatggcgg
     1021 tggtggcctg gtcgctcgtc gtttcgagct ggtcgagcag ggtcgatccg cgctcactca
     1081 aggcatcgat cattgaatcg ccggcatggc cgagcgccaa ggtaatgtgc tcgccttttt
     1141 cggtcaggga gcgggtgacg cgttgggcga cttcattgac cttgtcggcg accagatcgc
     1201 caaccgaagt aatgtcgtgc gcaagatcaa ggtgaacact ggcgatggcg ttgcggacct
     1261 gctcagcctg attgacaagc gtctcacgct ggttgctcag ctcggtgagc aggtcgcgga
     1321 tgcgtacctc gttgtcgttg taggctcgct cgagtgcgga gacttcgttg tggacgagcg
     1381 cttcaagctc ggccgcgcgg gcaagggcac gttcgacccc gtcacccatg gccgcgactt
     1441 cgcgacggat ggcctgcccg acagtgacga tggactcgcg ggcgaccgtt tcgggctgtg
     1501 ccatgcgcat cgccacttcg gccatcgatt ctgcaacgac gcgcatgtcc tgcgcgcggt
     1561 tgaacatatg cgcgagcacg aaaaagaaga cgatgggaac gacgatcgcc cccccaatcg
     1621 caagcaaggc cgcaaagccg agcttcgccg tcccgaacac tgcctgcgtg tcgatggcat
     1681 agagcgcggc gacgctgacg gcagcccaaa caagaccggc aattccggcc gcaatgtagg
     1741 gaccgcgcga cgagcgatga cgcagcgtct gcaggatctg gccgatgccg gcgcggtcat
     1801 cattggccgc gcggcgcggt gtggcctcgc cagagggcca gccactttgc ggcccgtctt
     1861 ggggaaagag gtcacctggc tcatgcgcgg attgcggatc ggggagaccg gcttctgcgg
     1921 gtccaaagga ggggccggca cgtgtatccg gcgggcgtat attgagggct tcctggatgg
     1981 cggtcagcgc ttcttcggtt gcgtctttgg ttttctgagg gatattcgcc atatcggtcg
     2041 tcgcctcgtc tttacgccgg actgtcgccg cggcccgaac cgtcaagaaa tcagggagtt
     2101 aagccgagaa cacggcaccg tggcggcagc tgcagccgcc cgcatccccg atttattgcc
     2161 cggcgaccat cctatcgcct tggcaacacg aaggaaacaa cgccggttag gacttttttt
     2221 atcatcgtta accgattgct aaccatagca aacggcgccg gtcggattgg gcagatcatg
     2281 tccggtgaat tgaggcagcg gtacggctgg accacgggtt gcgaacagaa gttggcgccg
     2341 ccgcttacgc gcacttaacc accgtcgtct ttggccgcct tctatttacc gggatttgat
     2401 cccgatcggg tcaaactctt cgccaataaa aacaacaccg ggagggcagg atggcgcaag
     2461 ccgcagtcca gatatccgag cgggtacagg ccgacgcacc gcaggttgcg gttctcgacc
     2521 gtgctcatct tgcgcgcatg acattcggcg accgcagcct ggaactggaa gtgcttcagt
     2581 tgttcgatcg tcaagccgaa ctgctgctcg atcgcatgcg cgggagccag ccggctgcaa
     2641 tcgtaacgct cgcccacacg ctcaaaggat cggctgtcgg aatcggtgcg acacaggttg
     2701 cccgtgccgc cgccgagacc gaggcgatcg cacacgcaaa gcccggtgaa tgcaatcggg
     2761 cgattgccca gcttgcgcag gcggtgaaag aggctcgggc ggagatttcc gccctgatcc
     2821 gcgctcattg attggggatg gcgtccccaa gggaatgact atataggaag cgcgcgcctg
     2881 ctggcgcggt cactcctgcc aagcttcctc atgccgaaga tcacttttac cgactccagc
     2941 ggcgccgccc gcacagtcga ggccgagaac ggctcgaccg tgatggaaac cgccatccgc
     3001 aacaatattc ccggcatcga ggccgaatgc ggcggtgcct gcgcgtgcgc aacctgtcat
     3061 gtctatgtcg acgaggcctg gcgtgaaaag gtcggcgggc cgtcgccgat ggaagaagac
     3121 atgctcgact tcggctacga cgtgc
//
LOCUS       DASSRL010000225         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1545743, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000225 DASSRL010000000
VERSION     DASSRL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545743"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /gene="surE"
                     /locus_tag="VFL49_04620"
     CDS             complement(<1..373)
                     /gene="surE"
                     /locus_tag="VFL49_04620"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET7911289.1"
                     /translation="MRILITNDDGIHSPGLAACLEIARALSDEVWIVAPEIDQSGVSH
                     SLSLNDPLRMRDVGERHFAVKGTPTDCIIMGVRHIMKKKPDLVLSGVNRGRNCAEDVT
                     YSGTVAGAMEGTVLGIPSFALS"
     gene            complement(373..1650)
                     /gene="serS"
                     /locus_tag="VFL49_04625"
     CDS             complement(373..1650)
                     /gene="serS"
                     /locus_tag="VFL49_04625"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7911290.1"
                     /translation="MYDIKWIREHPDAFDRGLKRRGLEPLSKKLVALDERRRAAITKF
                     EQAQARRNAASKEIGDAKKTNDEARAGKLMAEVAELKTSLPAMEDEQKRLTVALEKEL
                     AQIPNTPLDDVPDGTDASGNVEHHVSGKKRDYSFKPKQHFDLGEALGQMDFELAAKLS
                     GARFVVLQKGLARLERALGHFMLDVHTSKEHGYTEVSPPILVRDDVMFGTAQLPKFAD
                     DQFRTTNDLWLIPTAEVPLTNLARESIVEEAQLPMRLTACTPCFRAEAGAAGKDTRGM
                     IRQHQFTKVELVSITTPDRSKDEHERMLACAEEVLKKLDLHYRVVTLCTGDMGFAAQK
                     TYDIEVWLPGQNAYREISSCSNCGEFQARRMNARYRAKGGVRHVHTLNGSGVAVGRAL
                     IAVMETYQQDNGSIAVPSALAPYMGGLKKIERT"
     gene            complement(1801..2619)
                     /gene="tatC"
                     /locus_tag="VFL49_04630"
     CDS             complement(1801..2619)
                     /gene="tatC"
                     /locus_tag="VFL49_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7911291.1"
                     /translation="MTHEDIEATKAPLMDHLIELRSRLIKALLAFGVMFAICFVFAKD
                     IYNVLVWPFVWVAGPENSKFIYTALLEYFITQLKLAMFGAAFLSFPVVAAQIYMFVAP
                     GLYRHERKAFLPYLIATPIFFALGAMVVYFLVLPMLVRFSLHMQQPGSTTDASIALMP
                     KVGEYLSLMMALVLAFGVAFQLPVVLTLLGRIGVITAEQLKAKRRYFIVGAFVLAAVL
                     TPPDVLSQMSLALPLLILYEGSIWSVRMVEKKAQAEAAAKAAAEAASASAKPAE"
     gene            complement(2616..>2836)
                     /locus_tag="VFL49_04635"
     CDS             complement(2616..>2836)
                     /locus_tag="VFL49_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911292.1"
                     /translation="NKEIASAFTDKPAEPAAQDSAPSAETPLPEISVPLPEPLPPVTN
                     ADFAVPPEPLKEDARDLLPPSPKVEGAA"
BASE COUNT          560 a          868 c          848 g          560 t
ORIGIN      
        1 gtgagagcgc gaaggacggg atgccgagca cggtaccttc catggcgccc gcgacagttc
       61 cggaataagt gacatcctca gcgcaattac ggccgcgatt gacgccggac aggaccaggt
      121 cgggcttctt tttcatgata tgccgcacgc ccatgatgat gcagtcggtt ggcgtgccct
      181 ttaccgcaaa atgacgctcg ccgacgtccc gcatgcgcag cggatcgttc agcgagaggg
      241 agtgcgacac gcccgactga tcgatttccg gcgccacgat ccacacctcg tcggaaagcg
      301 cgcgtgcaat ttccaggcag gctgccagtc ccggcgagtg aatgccgtca tcgttggtaa
      361 tgagaatacg cattacgtcc gctcgatctt tttcaagccg cccatatatg gcgccagcgc
      421 cgacggcacg gcaatcgagc cgttgtcctg ctgataggtt tccatgaccg caatgagtgc
      481 gcgtccgaca gcaacgccgg agccgttgag tgtatgcacg tgcctcacgc cacccttggc
      541 gcggtagcgc gcgttcatgc gacgggcctg aaactcgccg cagttcgagc aggacgaaat
      601 ctcacggtag gcattctgcc ccggcagcca gacctcgatg tcgtaggtct tctgcgcggc
      661 aaagcccatg tctccggtgc agagtgtgac gacccgatag tgcaagtcga gcttcttgag
      721 gacctcttcg gcgcaggcaa gcatgcgctc atgctcgtcc ttcgatctgt cgggggtcgt
      781 aatagaaacc aactcgactt tggtgaactg gtgctgccgg atcatgccgc gcgtgtcttt
      841 gcccgcggcc ccagcttccg cgcggaagca tggcgtgcac gcggtcagcc gcatcggcaa
      901 ttgcgcttcc tcgacgatgg attcgcgcgc aagattggtg agcggcactt cggcggtcgg
      961 gatgagccag agatcatttg ttgtccggaa ttggtcatca gcgaacttcg gtaattgcgc
     1021 cgttccaaac atgacgtcat cgcgaaccaa aattggcggg cttacttcag tgtacccgtg
     1081 ctccttgctt gtgtgcacat caagcatgaa atggccgagc gctcgttcga gtcgcgccag
     1141 gcctttctgc aagacaacaa aacgtgcccc ggaaagtttt gccgcaagct cgaaatccat
     1201 ttgcccgagc gcctcgccca gatcgaaatg ctgcttcggc ttgaatgaat agtcgcgctt
     1261 cttgccggat acgtgatgct cgacattgcc gctcgcgtcg gtgccatccg gcacgtcgtc
     1321 gagcggcgtg ttgggaattt gtgcgagctc cttttcgagt gcaaccgtca atcgcttctg
     1381 ctcgtcctcc atcgccggca acgaggtctt gagctcggca acctcggcca tcagcttgcc
     1441 ggccctggcc tcgtcgttcg tctttttcgc atcgccgatt tccttcgacg cggcgttacg
     1501 gcgcgcttgc gcttgctcga acttggtgat ggccgcccgg cgcctttcat cgagcgccac
     1561 gagctttttc gacagcggtt caagcccgcg ccgtttcagc ccacggtcga aggcatcggg
     1621 atgctcgcga atccacttga tgtcgtacat ggcgggcgct ctttagcacg tcggggccgg
     1681 gcaaaaggca tgaagtgacg tcttggtgtc acttgtcccg gccatccacg tctttccaat
     1741 aatgaagacg tggatgcccg gcataaagcc gggcatgacc aagtcaagac cagccgccgt
     1801 ttactccgcc ggcttggcgc tcgcggaggc tgcctcggct gccgcttttg ccgcggcctc
     1861 ggcctgcgcc tttttctcga ccatgcgcac cgaccagatc gagccctcgt aaaggatgag
     1921 cagcggaaga gcgagtgaca tctggctgag cacatccggc ggggtcagaa cggctgccag
     1981 aacaaaggcc cccacgatga aataacgcct tttggccttg agctgctcgg cagtgattac
     2041 cccgatgcgt ccgagcagcg tcagcacaac cggcaactga aaggcaacgc caaaggcaag
     2101 aaccagcgcc atcatcagcg acagatactc gccgaccttc ggcatcagcg cgatcgaagc
     2161 gtcggttgtc gagcctggtt gctgcatgtg cagcgaaaag cgcaccagca tcggcaacac
     2221 gagaaagtag acgaccatcg cgccaagcgc gaagaaaatc ggcgtcgcga tcaggtatgg
     2281 gaggaacgcc ttgcgctcgt ggcgataaag ccccggcgca acgaacatgt agatctgcgc
     2341 tgcgaccacc ggaaaagaca ggaaggccgc gccgaacatc gcgagcttca gctgcgtgat
     2401 gaagtattcc agcagcgcgg tatagatgaa ttttgaattc tcggggccgg caacccacac
     2461 gaagggccac accaggacgt tgtagatgtc cttcgcaaaa acgaagcaaa tcgcgaacat
     2521 cacgccgaac gccaacaacg ccttgatcag ccgcgaccgc agctcgatca ggtgatccat
     2581 cagcggggcc ttggtggcct cgatgtcctc gtgggtcatg ccgcgccctc gaccttcgga
     2641 ctcggcggca ggagatcgcg ggcgtcttcc ttcagtggct caggcggaac ggcgaagtcg
     2701 gcattggtga ccggcggcaa cggctcgggc aaagggacag aaatctccgg tagcggcgtt
     2761 tccgcagacg gcgcgctgtc ttgcgctgcc ggttcggccg gcttgtccgt gaacgccgac
     2821 gctatctcct tgttgg
//
LOCUS       DASSRL010000226         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1685841, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000226 DASSRL010000000
VERSION     DASSRL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685841"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFL49_04640"
     CDS             complement(<1..903)
                     /locus_tag="VFL49_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7911293.1"
                     /translation="MALSERLNTPISNAELDRRWRAVRDAMEKEKIDVLLMQNNNDHM
                     GGYVRWFTDMPATNGYPNTVVFPRDDEMTVVCQGPFGGGEATAQGDQNWRGVKRILTT
                     PSYASAHYTKEYDPELAATALKPFVNGVIGYVGTYQMSYATLDSIKRAFPQARFVDAT
                     DMIDRIKVIKSPEEMELVKRAALMQDGAMRAAFAAVKPGMRDIEVAAVAQHYSLCHGS
                     ENGIYLCASMPLGNPSKFANRHLQNRVIQKGDQIALLVEDNGPGGMYTELGRSCVVGK
                     VPQQMKDELGFCKASRKMTLDLLKP"
     gene            complement(908..1879)
                     /locus_tag="VFL49_04645"
     CDS             complement(908..1879)
                     /locus_tag="VFL49_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET7911294.1"
                     /translation="MPNVLILTEVQEAMRARYKAMLLERFPQLTIDVVGHHNDVGPYI
                     ADVDILLCFSPPMADHVVRDASKLKWIQALGTGVDNIVDLPSLGKEVLVTNVRGIHGA
                     PVSEATIAFMLSLARDMPRSVRAQDQSRWERWPSALLAGKTVGIFGVGLIAEYLAPIC
                     KTFGMTVVGISGSPRETKHFDRMAHRNDLTKIAGDLDFLVVLTPLTAETRDIVGEKVF
                     AAMKPTAYLVNVARGGVVDEPALIAALEAGQIAGAGLDVFSQEPLPSDNPLWKTKNLT
                     IFSHLGGYSQGYEDRAMPTIAGNMGKFLAGDLKSMINIVRKPASWKD"
     gene            1968..2345
                     /locus_tag="VFL49_04650"
     CDS             1968..2345
                     /locus_tag="VFL49_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911295.1"
                     /translation="MDSMPIDLAQVDWLYVILLAVFVAISTFLGGLLSFRRRGTAAVL
                     SAVLFAVIFVFWTYYPHRVPFLPRTLTTAQDTAKQTPAPPPAAPATSQKPANPVRDIT
                     PPNNPVTDVTPPAPAGPPAAPAR"
     gene            2411..3112
                     /locus_tag="VFL49_04655"
     CDS             2411..3112
                     /locus_tag="VFL49_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HET7911296.1"
                     /translation="MKKVYPDANAAMAGLLKDGMLICCGGFGLCGIPETLIKGIRDSG
                     VKNLTIVSNNAGVDGAGLGLLLETRQVKKMISSYVGENKLFAQQYLAGELEIEFNPQG
                     TLAERIRAGGAGIPAFFTRTGAGTDIAKGKEEREFDGSRYIMERGIVADLSIVHAWTG
                     DTEGNLVYRKTARNFNPMMATAGKMTIAEVENLVEPGKLNPDHIITPGVYVKRIVHVP
                     NAVKHIEQRTVRKRA"
     gene            3123..3773
                     /locus_tag="VFL49_04660"
     CDS             3123..3773
                     /locus_tag="VFL49_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HET7911297.1"
                     /translation="MPWTREQMAERAAKELRDGFYVNLGIGIPTLVTNYIPAGMTVQL
                     QSENGMLGFGPFPYEGEEDPDLINAGKQTVTELPTTSFFSSADSFAMIRGGHIDLSIL
                     GAMQVAENGDLANWMIPGKMVKGMGGAMDLVAGVKKVVVVMEHTAKDEPKLLHRCKLP
                     LTGAGVVDMVITDLGVFTIDKKAGGMTLTELAPGVTVDEITKKTEADFKVDPKLKH"
     gene            3811..4125
                     /locus_tag="VFL49_04665"
     CDS             3811..4125
                     /locus_tag="VFL49_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911298.1"
                     /translation="MTREPDFDEEMDRLQGHMPDWAGRNFDRLRQPEAVWVRVPAGIA
                     LTGGGVLSFLPVLGIWMLPVGLALLACDVQPMQPPLARVLRFTNRKIEERKERKSDKK
                     AD"
     gene            4192..>4851
                     /locus_tag="VFL49_04670"
     CDS             4192..>4851
                     /locus_tag="VFL49_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioate hydratase"
                     /protein_id="HET7911299.1"
                     /translation="MLSDADRKKAADILMAAQKEHKQAVQLHVTFPDITIEDSYAIST
                     DVMQRKLRAGAKLIGHKVGLTSKAMQRSSMIDEPDYGYILDDQLIADGAKVNHADYCK
                     PRVEVELAFIMGRKLQGPGVGLTDVLRATEYVVPAIEIVDARIQDPRKIVDTVADNGA
                     AAGIAMGGRPVGPLDIDLRWVGGIMHRNSEIEETGLAAGVLGHPALGVAWLANKIGPH
                     GH"
BASE COUNT          947 a         1454 c         1536 g          914 t
ORIGIN      
        1 gggcttcaac agatcgagcg tcatcttgcg cgatgccttg cagaacccca attcgtcctt
       61 catctgctgc ggcaccttgc cgacgacgca ggagcggccg agctcggtgt acatgccgcc
      121 gggcccattg tcctcgacca gcagcgcgat ctgatcgcct ttctggatga cacgattctg
      181 caggtgacgg ttggcaaact tggatggatt gccgagcggc atcgacgcgc aaagataaat
      241 gccgttctcg ctgccgtggc agaggctgta gtgctgcgcg acggcggcga cctcgatgtc
      301 gcgcatgccg ggcttcaccg cggcgaaggc cgcacgcatg gcgccgtcct gcatcagcgc
      361 ggcgcgtttc accagttcca tctcttccgg actcttgatg accttgatgc ggtcgatcat
      421 gtcggtggcg tcaacgaaac gtgcctgcgg aaaagcgcgc ttgatactgt cgagcgtcgc
      481 ataggacatc tgataggtgc cgacataacc gatcacgcca ttgacgaagg gcttgagcgc
      541 ggtcgcggca agctcggggt catactcctt cgtgtaatgt gcggacgcgt aactcggcgt
      601 cgtgaggatg cgtttgacac cgcgccagtt ctgatcgccc tgcgcggtcg cctcgccgcc
      661 gccgaaggga ccctggcaca ccacggtcat ttcatcgtcg cgtggaaaga ccacggtgtt
      721 ggggtagccg ttggtggcgg gcatgtcggt gaaccagcga acatagccgc ccatgtgatc
      781 gttgttgttc tgcatcagca ggacgtcgat cttttctttt tccattgcgt cgcgcacggc
      841 gcgccagcga cggtcgagtt cggcattgga gatcggcgtg ttgaggcgtt cggacaaggc
      901 catggtttca gtctttccag gatgcgggct tgcgcacgat attgatcatg gatttgagat
      961 cgccggcaag aaatttgccc atgttgccgg cgatcgtcgg catcgcacgg tcttcataac
     1021 cctgcgaata gccgccaagg tgcgagaaga tcgtcaggtt ttttgtcttc cacaatggat
     1081 tgtcggacgg cagaggctcc tgcgagaaga cgtcgagccc ggcgccggcg atttgtcccg
     1141 cttcaagggc tgcgatcaag gccggctcat cgacaacgcc gccgcgcgca acattgacca
     1201 gataggccgt cggcttcatg gcggcaaaga ctttctcgcc gacaatgtcg cgcgtttcgg
     1261 ccgtcagcgg agtgagcacg accaggaagt caagatcgcc ggcgatcttg gtcaagtcgt
     1321 tgcgatgcgc catgcgatcg aaatgctttg tttcgcgcgg gctgccgctg atgccgacga
     1381 cggtcattcc gaaggtcttg cagatcggcg cgaggtattc ggcgatcagc ccgacgccga
     1441 aaatgccgac ggtcttgccc gcgagcagcg cggagggcca gcgctcccac ctggattggt
     1501 cctgggcgcg caccgagcgc ggcatgtcgc gggcaaggga gagcatgaag gcgatcgtag
     1561 cttcggacac cggcgcgcca tggatgccgc gcacgttcgt caccagcacc tcttttccga
     1621 gcgaggggag gtcgacgatg ttgtcgacgc cggtgccgag cgcctggatc catttcagct
     1681 tcgacgcgtc gcgtacgacg tgatcggcca tcggcggcga aaagcacaac aggatatcga
     1741 catcggcgat gtaaggcccc acatcattgt gatggccgac gacgtcgatg gtcagttgcg
     1801 ggaagcgctc gagcagcatc gccttgtagc gcgcgcgcat ggcttcctgg acctcggtaa
     1861 gaataaggac attcggcatg gggcgcaggt atagcgtgtg atctcgaacg aggggaaccg
     1921 gagtttcacg tgacccgcat cgcgggtctg ggataaggtg gaaagcgatg gattcaatgc
     1981 cgatcgatct tgcgcaggtg gactggctct acgtcatcct gctggccgtc tttgtcgcca
     2041 tttcgacttt tctcggcggt cttctgtctt tcaggcgccg cggcacggct gcggtgctgt
     2101 cggcggtgct ttttgcggta atcttcgtat tctggaccta ctacccgcat cgcgtgccgt
     2161 tcctgccgag gacgctcacg acggcgcaag acaccgccaa gcaaacgccg gccccgccgc
     2221 ccgcggctcc tgcaacttcg caaaaaccgg cgaacccggt ccgcgacatt accccgccca
     2281 acaatcccgt caccgacgtc acgccgccgg cacccgccgg cccgcctgcg gcaccggcgc
     2341 gctagacttg catccggcca gccgaatgat gttttctcca atttcctgaa agacgaaaac
     2401 ggtagcttca atgaaaaaag tttatcccga cgcaaatgcc gcaatggcag gcctgctcaa
     2461 ggacggcatg ctgatctgct gcggagggtt cggcctctgc ggaattccgg agaccttgat
     2521 caagggtatt cgtgacagcg gcgtcaagaa tttgaccatc gtttccaaca atgcgggcgt
     2581 cgacggggcc gggctcggtc tgctgctgga gacgcggcag gtgaagaaaa tgatctcgtc
     2641 ctatgtcggc gagaacaagc tgttcgcgca gcagtatctc gccggcgaac tcgagatcga
     2701 attcaacccg caaggcacgc tggcagaacg catacgcgcg ggcggtgcgg gaattcccgc
     2761 tttcttcacg cgcacgggcg cgggcaccga catcgcgaaa ggtaaggaag agcgcgagtt
     2821 cgacggcagc cgctacatca tggagcgcgg catcgtcgcg gacctgtcga tcgtgcatgc
     2881 ctggacgggc gacaccgaag gcaacctcgt gtaccggaag acggcgcgaa atttcaaccc
     2941 gatgatggcg actgccggca agatgaccat tgccgaagtc gagaacctcg tcgagccggg
     3001 caagctcaac ccggatcaca tcatcacacc tggcgtctac gtcaaacgca tcgtgcacgt
     3061 gccgaatgcg gtcaagcaca ttgagcagcg caccgtgcgc aagagagcct agaggaacaa
     3121 tcatgccctg gacccgcgag cagatggccg aacgcgccgc caaggaattg cgcgacggct
     3181 tctacgtcaa tctcggcatc ggcataccga cgctggtgac gaactacatt cctgcaggca
     3241 tgaccgtgca gctgcagagc gagaacggca tgctcggctt cgggcctttc ccctatgaag
     3301 gggaggagga ccccgatctg atcaatgccg gcaagcagac cgtcaccgaa ttgccgacca
     3361 ccagcttttt ctcgtcggcc gattccttcg ccatgatccg cggcgggcat atcgacctgt
     3421 cgatccttgg cgcgatgcag gtcgcggaaa acggcgacct cgccaactgg atgattcccg
     3481 gcaagatggt gaaaggcatg ggcggcgcga tggatctggt cgccggcgtg aagaaggtcg
     3541 tcgtggtgat ggagcacacc gccaaggacg agccgaaact gctgcacagg tgcaagctgc
     3601 cgctcaccgg cgccggcgtg gtcgacatgg tgatcaccga tctcggcgtc ttcaccatcg
     3661 acaagaaagc cggcggcatg acgctgaccg agctcgcgcc cggcgtgacc gtcgatgaga
     3721 tcacaaaaaa gaccgaggcg gatttcaaag tcgatccgaa gctcaagcac tagggccgga
     3781 acaattccgc gcctgggcct ttgatctggc atgacgcgcg agcccgactt cgacgaggag
     3841 atggatcggt tgcagggcca catgcctgac tgggcaggcc gcaatttcga ccgtttgcgt
     3901 cagccggagg cggtgtgggt gcgtgtgccg gccgggatcg cgctgaccgg cggcggtgtc
     3961 ctgagcttcc tgccggttct cggcatctgg atgctgccgg tcggtctggc gctgctggct
     4021 tgcgatgtgc agccgatgca accgccgctc gcccgcgtgc tgcgttttac caaccgcaag
     4081 atcgaggaaa ggaaagagcg caagtccgac aaaaaggcgg actagccggc ttcctccctt
     4141 tgattttctt gtgcgaaccg ttacagcatc ggtctgaccg ggggatgatc catgctgtcc
     4201 gacgccgacc gcaagaaagc tgccgacatc ctgatggcgg cgcaaaaaga gcataagcag
     4261 gcggtacagc tccacgtcac gtttccggac atcaccatcg aggattccta cgcgatttcc
     4321 actgacgtga tgcagcgcaa attgcgggca ggcgccaagc tgatcggcca caaagttggc
     4381 ctcacgtcga aggctatgca gcgctcgtcg atgatcgacg agcctgacta cggatacatt
     4441 ctcgacgatc agctgatcgc cgatggcgcc aaggtcaatc acgccgacta ctgcaagccg
     4501 cgggtggagg tagagcttgc cttcatcatg ggcaggaagc tgcagggacc cggtgtcggt
     4561 ctcaccgatg tcttgcgcgc caccgaatat gtagtgccgg cgatcgagat cgtcgacgcg
     4621 cgtatccagg atccgcgcaa gatcgtggat acggtcgcgg acaatggcgc cgccgccggc
     4681 atcgccatgg gcggtcgccc ggtcggcccg ctggacattg atctgcgctg ggtcggcggg
     4741 atcatgcacc gcaattccga gatcgaggag acggggctgg ctgccggcgt gctcggtcat
     4801 ccggcgttgg gcgtggcctg gcttgccaac aagatcggtc cgcacggcca c
//
LOCUS       DASSRL010000227         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_705778, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000227 DASSRL010000000
VERSION     DASSRL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705778"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VFL49_04675"
     CDS             <1..147
                     /locus_tag="VFL49_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit E"
                     /protein_id="HET7911300.1"
                     /translation="DYYEDLTAESFGKILDDLAAGKKAKPGPQIDRQLSAPIGGATTL
                     KEKA"
     gene            151..1470
                     /gene="nuoF"
                     /locus_tag="VFL49_04680"
     CDS             151..1470
                     /gene="nuoF"
                     /locus_tag="VFL49_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7911301.1"
                     /translation="MLADKDRIFRNLYGQHDWGLKGARARGAWDGTKAILEKGRDAII
                     DEVKTSGLRGRGGAGFPTGLKWSFMPKKSDGRPHYLVVNADESEPGTCKDREIMRHDP
                     HLLVEGCLIAGFAMGANAGYIYVRGEFIRERERLQAAVDEAYEAKLIGKNNANGWDFD
                     LYVHHGAGAYICGEETALLESLEGKKGQPRLKPPFPANVGLYGCPTTVNNVESIAAVP
                     DILRRGASWFAGIGRPNNTGTKLFCISGHVERPCNVEEAMGIPLRELLETHAGGVRGG
                     WDNLLAVIPGGSSMPLVPAGKDWADSLLMDFDGCRDKKSALGTAAVIVMDKSTDIVRA
                     MARISYFYKHESCGQCTPCREGMGWMWRVLTRMAEGRAQKREIDMLMQVTQQIEGHTI
                     CAFGDGAAWPVQGLLRHFRPEIERRIDEYSRNADSHGAREPVPQAAE"
     gene            1470..1970
                     /locus_tag="VFL49_04685"
     CDS             1470..1970
                     /locus_tag="VFL49_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HET7911302.1"
                     /translation="MAMQQPANHHLAQLNIGRIRYEIEDPRMADFTNNLAMVNGLAER
                     TPGFVWRYIDESGNSTSTRPYADPRIANNRSVWENIEALERFVYQTVHKRFYGRRAEW
                     FEHFEGPYFVMWWVPAGHRPAVKEAVARLEYLKQHGASDHAFAWQATDMQLWKTARCA
                     PSESAA"
     gene            1967..2458
                     /locus_tag="VFL49_04690"
     CDS             1967..2458
                     /locus_tag="VFL49_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HET7911303.1"
                     /translation="MTTELRHLAQFNIARIRYPLDDPRMREFVENVDRVNRLADKIEG
                     FVWRLQDESGHAMNMRVYDDPTILPNLTVWESVEALERFVWQTLHRRFFLRRQEWFAP
                     IETTLVLWWVSAGHRPGMTEGVARLDHLIAHGPGDHAFGWRQLPGAKLWKTARVNQPE
                     YAV"
BASE COUNT          530 a          813 c          883 g          462 t
ORIGIN      
        1 gactactacg aagacctgac ggcggagagc ttcggcaaga tcctggatga tcttgcggcg
       61 ggcaagaaag cgaagccagg cccgcagatc gaccggcagc tttcggcgcc gatcggcggc
      121 gcaaccacgc tcaaggaaaa ggcgtgagcg atgctggctg acaaggatcg gatcttcaga
      181 aacctgtacg gccagcacga ctggggcctg aagggcgcgc gtgcacgtgg cgcctgggat
      241 ggcacgaaag ccattctcga aaaaggccgc gacgcgatca tcgacgaagt caagacgtcg
      301 ggcttgcgcg ggcgcggcgg cgccggcttt ccgacgggac tgaaatggtc gttcatgcca
      361 aagaagtcgg acgggcgtcc gcattatctc gtggtcaacg ccgacgaatc cgagccgggc
      421 acctgcaagg accgcgagat catgcggcac gatccgcacc tcctggtcga aggctgcctg
      481 atcgccggct tcgccatggg cgccaacgcc gggtacatct atgtgcgcgg cgagttcatc
      541 cgcgagcgcg aacggctgca ggcggcggtc gatgaagcgt acgaggcgaa gctcatcggc
      601 aaaaacaacg cgaacggctg ggacttcgac ctttacgtcc accacggcgc gggtgcctat
      661 atctgcggcg aggaaaccgc gctgctcgag agccttgaag gcaagaaagg ccagccgcgg
      721 ctgaagccgc cgttcccggc caacgtgggc ctttatggct gtccgaccac cgtcaacaac
      781 gtggaatcga tcgcggccgt tcccgacatc ctgcgccgcg gcgcctcatg gtttgccggc
      841 atcggacggc cgaacaacac cggaaccaag ctgttctgca tctccggcca cgtcgagagg
      901 ccgtgcaacg tggaagaagc aatgggcatt ccgctgcgcg agttgctcga gacgcatgcc
      961 ggcggcgtgc gcggcggctg ggacaacctg ctcgcagtca ttcccggcgg ctcgtcgatg
     1021 ccgctggtgc cggccggcaa ggactgggcg gattcgctgc tgatggattt tgacggctgc
     1081 cgcgacaaga agtcggcgct cggcacagct gccgtgatcg tgatggacaa gtcgacggat
     1141 atcgtgcgcg cgatggcacg catctcctac ttctacaagc atgagagctg cggccaatgc
     1201 acgccgtgcc gcgagggcat gggctggatg tggcgcgtgc tcacgcgcat ggctgaaggc
     1261 cgtgcgcaga agcgcgagat cgacatgctg atgcaggtga cgcagcagat cgaaggccac
     1321 accatctgcg ccttcggcga cggcgccgcc tggccggtac agggcctgct gcggcatttc
     1381 cgtcccgaga tcgagcgtcg catcgatgaa tattcgcgca acgccgacag ccacggcgcg
     1441 cgcgagccgg tgccgcaggc ggcggagtga tggcgatgca gcagccggca aaccatcatc
     1501 ttgcacagct caatatcggt cgcatccgct acgagatcga ggatccgcgc atggcggatt
     1561 tcaccaacaa tctggcgatg gtgaacggcc tcgccgagcg tacacccggt ttcgtgtggc
     1621 gctacatcga tgagagcgga aattcgacga gcacgcggcc ttacgccgac ccgcgtatcg
     1681 ccaacaaccg gtccgtctgg gaaaatatcg aggcgcttga gcgtttcgtc taccagacgg
     1741 tccacaagcg cttttacggc cgtcgcgcgg aatggtttga acatttcgaa ggcccgtatt
     1801 ttgtcatgtg gtgggtacct gccgggcatc gcccggccgt caaggaggcg gtcgcccgcc
     1861 tcgaatatct caagcagcat ggcgcgagcg atcacgcctt cgcctggcag gcgaccgaca
     1921 tgcagctttg gaaaacggcg cgttgcgcgc caagcgagtc ggcggcatga cgacagaact
     1981 ccgccatctc gcccagttca acattgcgcg gatccgctat ccgctcgatg acccgcgcat
     2041 gcgcgaattc gtcgagaatg tcgatcgggt gaacaggctg gcggacaaga tcgagggctt
     2101 cgtctggcgg ctgcaggatg aaagcggtca tgcgatgaac atgcgcgtct atgatgaccc
     2161 caccatcctg cccaacctga cggtctggga aagcgtcgag gcgctggagc gcttcgtctg
     2221 gcagacgctg catcggcgct tctttcttcg ccggcaagaa tggttcgcgc cgatcgagac
     2281 cacgctggtt ttgtggtggg tttctgccgg acatcgcccg ggcatgacag aaggtgttgc
     2341 gcggctcgat catctgattg cgcatggtcc cggtgaccat gcattcggct ggcggcagct
     2401 tcccggagca aaactgtgga agacggcacg cgtcaatcaa cccgagtatg ccgtatgagc
     2461 cattttgcaa ataatgggcg gcgatcggcg cacctggagc aaggcactgg tcgcaatggt
     2521 gcgcttcacg ctgcattcgt ggcgccactt tgcatgattg ccgcgccggt gcgcatccgg
     2581 aaggctgtcg catgaccaag atcattgtcg atggaaaaga aatcgacgtt ccgccggagt
     2641 acacgctgct ccaggcctgc gaggccgcgg gcgcggaaat tccgcgct
//
LOCUS       DASSRL010000228         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_425813, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000228 DASSRL010000000
VERSION     DASSRL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425813"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFL49_04695"
     CDS             <1..522
                     /locus_tag="VFL49_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HET7911304.1"
                     /translation="DGKPISGNQMVEQLFGTLPQFFKDEDQLRRLWSQPATRKALLQG
                     LAEKGFGSDQLAEISRLINAEKSDVYDVLAYIAFALTPVTRAERVERRKRAIFAKYNG
                     SLQSFLEFVLGQYVSQGVEELDQEKLGHLLELKYHTIDDAAAQLGGVPKIRDTFLGFQ
                     QYLYIDEPAKGTD"
     gene            654..1769
                     /gene="mtnA"
                     /locus_tag="VFL49_04700"
     CDS             654..1769
                     /gene="mtnA"
                     /locus_tag="VFL49_04700"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET7911305.1"
                     /translation="MRVNGKSYRTIWPADDGRTVRIIDQTLLPHRFEIVDLRDLAAAV
                     TAIKTMQVRGAPLIGATAAYGICLAMAADASDQSLDRTYDALLASRPTAVNLRWALDT
                     MRSHLRNRRREERVEAAYRRAAEICDEDVAINAAIGEHGLEILRGAAAKKSGKDPLNV
                     LTHCNAGWLATVDWGTALAPIYKAHDSGMPIHVWVDETRPRNQGMSLTAWELGQHGVA
                     HTVIADNAGGHLMQHGMVDLAIVGTDRTTARGDVCNKIGTYLKALAAKDNGVPFYVAL
                     PSPSIDWLIDDGVAGIPIEQRDASEVSRISGRLPDGTIVQVEIAPEGSAVANYGFDVT
                     PAHLVTALVTERGACDASSQGLRRLFPEGAAASRAHA"
     gene            1762..>2154
                     /locus_tag="VFL49_04705"
     CDS             1762..>2154
                     /locus_tag="VFL49_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HET7911306.1"
                     /translation="MRNLWSDRDARAAVKIYRAQGVNEDLALRTYSTRLLGGDPRLVQ
                     HGGGNTSVKTVMRDPAGEPAGVLCVKGSGWDMAAIEPAGLPAVRLAPLLKLAGLKALN
                     DEDMVAIQRGNLLDSAAPNPSVETLLHAF"
BASE COUNT          421 a          654 c          696 g          383 t
ORIGIN      
        1 gacggcaagc cgatttccgg caaccaaatg gtcgaacaat tgttcggtac gctgccgcag
       61 ttcttcaagg atgaagacca gttgcggaga ctctggagcc agcctgccac gcgcaaggca
      121 ctgctgcagg gactggccga aaagggattc ggctcggatc agcttgcgga aatcagtcgg
      181 ctcatcaacg cggaaaaaag cgacgtctat gacgtcctcg cctacattgc gtttgcgctc
      241 acgcccgtga cccgcgcgga acgcgtcgag cggcgcaagc gcgcgatctt cgcaaagtat
      301 aatggaagcc ttcagtcgtt tctggaattt gtgctgggcc aatacgtgag tcagggcgtc
      361 gaggaactgg accaggagaa gctcggccat ctgcttgagc tgaaatatca cacgattgac
      421 gacgcggcag cgcagcttgg aggggttccg aaaattcgcg atacatttct gggctttcag
      481 cagtatttgt acattgatga gcctgcaaaa gggactgact agtcgtaagc gcgcatctca
      541 tccggccatg caagcgagag tctgcaggcg gcctttaggc agacgatcat gcttggcttg
      601 cacaattctt tcgcgagatg ccgcaccaaa cagtctcagc tataatggtt tccatgcgcg
      661 tgaacggcaa atcctaccgc acaatctggc cggcggacga cggcagaacg gtccgcatca
      721 tcgaccagac cctgctgccg caccggttcg aaatcgtcga cctgcgcgat ctggctgccg
      781 ccgtgaccgc gatcaagacc atgcaggtgc gcggtgcgcc gctgatcggc gcgactgccg
      841 cgtacgggat ttgcctggcg atggccgccg acgcgtcgga tcagtcgctc gatcggactt
      901 acgacgcgtt gctggcgagt cgcccgacgg cggtgaattt gcgatgggcg ctcgatacga
      961 tgcgttccca tctgcgcaat cgccggcgcg aggaacgcgt cgaggcggcg taccgccgcg
     1021 ctgccgaaat ttgcgacgag gacgtggcga tcaacgccgc catcggcgaa cacggtcttg
     1081 aaattctgcg tggcgccgcc gcgaagaaat ccggcaaaga tccgctcaac gtgctcacgc
     1141 attgcaatgc gggctggctc gcgacggtcg attggggcac ggcgctggcg ccgatctaca
     1201 aggcgcacga cagcggcatg ccgatccatg tctgggtcga cgagacgagg ccgcgcaatc
     1261 agggaatgag cctgaccgcg tgggagctgg gacagcatgg cgtcgctcac accgtcatcg
     1321 ccgacaatgc aggcgggcat ctgatgcagc acggcatggt cgacctggcc attgtcggaa
     1381 cggaccgcac gacggcgcgc ggcgacgtct gcaacaaaat cggcacgtat ctcaaagcgc
     1441 ttgcggcgaa agacaatgga gttccgttct atgtcgcgct gccgtcgcca tcgatcgact
     1501 ggctgatcga cgacggtgtt gcgggcatac ccatcgagca gcgtgatgcc tcggaagtct
     1561 cccgcatcag cggacgtctt cccgacggga caatcgtgca ggtcgagatc gcgccggaag
     1621 gcagcgcggt tgccaattac ggcttcgatg tcacgcctgc gcatctcgtt acggcgctgg
     1681 taaccgaacg cggggcgtgc gatgcctcgt cacaaggatt gcgccggctc tttccggaag
     1741 gcgcggccgc gagcagagcc catgcgtaac ctgtggtcgg accgcgatgc gcgggctgcg
     1801 gtcaagatct atcgcgcgca gggcgtaaac gaggacctcg cgctgcgcac atattcgacc
     1861 cgcctgctgg gcggcgatcc gcgcctggtt cagcacggcg gcggcaatac ctcggtcaag
     1921 accgtcatgc gcgatccggc cggtgagcct gcgggcgtcc tgtgcgtgaa aggctccggc
     1981 tgggatatgg cggcaatcga gccggccggc ttgccggcgg tgcggctcgc accgctgttg
     2041 aagctcgccg gcctcaaggc cctcaacgac gaggacatgg tcgcgattca gcgcggcaat
     2101 ttgctcgaca gcgccgcgcc caatccgtcg gtcgagaccc tgctgcacgc gttc
//
LOCUS       DASSRL010000229         5499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1055869, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000229 DASSRL010000000
VERSION     DASSRL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055869"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFL49_04710"
     CDS             <1..621
                     /locus_tag="VFL49_04710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7911307.1"
                     /translation="AVLKALADQCTKALFFPIGKHAGWHPEILKQVAAAGHTVGSHTW
                     SHKDLSKLSQQEGIDEIEKGIAAVSIALGNKPTAPFFRFPALRHPPELLKYVGERNIG
                     VFSTDLDSFDFKMRKPDQVIASVMKKLEKHGKGIILMHDFQQATAHAAPELLKKLKDG
                     GYKVVQVVGKTPLEPKKEYVDMVLKEMGGGMESARPMSSVIKDISN"
     gene            complement(851..1681)
                     /locus_tag="VFL49_04715"
     CDS             complement(851..1681)
                     /locus_tag="VFL49_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7911308.1"
                     /translation="MIDPLYSLSGFAVGLLVGMTGVGGGALMTPILIMLFGVHPATAV
                     GTDLLYAAATKTGGSLVHGFARSIDWRVVGRLATGSIPATILTLAALSHFNLSGDAAR
                     NLITLVLSIALFATAFVLVFGASIVRVYRARVVDLDPERTATITIVVGAVLGCLVSIS
                     SVGAGAMGVIALVMLYPHLPMAKIVGSDIAHAVPLTLIAGLGHWMMGSVNWAIIGSLL
                     AGSLPGIFVGSYFAIRIPERALRLVLATMLFFVATRIAYEHATAASSIFTAFSGRAVP
                     "
     gene            1889..2842
                     /locus_tag="VFL49_04720"
     CDS             1889..2842
                     /locus_tag="VFL49_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET7911309.1"
                     /translation="MADIVTLTINPAIDIFVNVGRVEPTRKMRCSAPKRDPGGGGINV
                     ARVAHRLGGRVTAIFPTGGAIGKLLHRLVDREGIDSIVTPSHVETRENFTAYEESTGE
                     QYRFVLPGSTLHRAEWETVLEKLTTLSQRPKFVVASGSVPPGVPDDFFARVARHAKML
                     GAQCVIDTSGPALKAALAEGVSLIKPNLVELSDLVGATLGNDEVRLRACRKLIEDGRV
                     KIVALTLGEDGALLVTADQTFRAEPMAIEPVSTVGAGDSFVGGLIAALASGESLAQAF
                     RVAVAAASAAVMSPGTELCRHEDVQRLLPQVKISELARATA"
     gene            complement(3081..3509)
                     /locus_tag="VFL49_04725"
     CDS             complement(3081..3509)
                     /locus_tag="VFL49_04725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7911310.1"
                     /translation="MKPIYILNGPNLNRLGKREPSIYGKATLADVERFCRDEAGDYPV
                     EFRQSNSEAELIDWIHEAIDMGGGIIINPAAFTFTSIAILDALKMFEGPIIELHISNI
                     HKREAYYHHSFVSKVATAVIAGFGPEGYRIATSSMKKLLK"
     gene            complement(3522..4823)
                     /locus_tag="VFL49_04730"
     CDS             complement(3522..4823)
                     /locus_tag="VFL49_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7911311.1"
                     /translation="MTMPSPFAICIVAIVALGALGLPIGHAMIAGSILYLLLSGLDLG
                     TAAEQILNGLFNSYVLLAIPLFILAADLMNIGSLTDRLLQFCLVLVGRFRGGLGHVNV
                     VANMIFAGMSGSAIADAVGIGRIIIGMMTREGRYPVAYAGAITASAAIIGPIIPPSIP
                     MVLYALISDTSIGYLFLGGVVPGVMLGIAFMVANSVIARRRHYPVEPPVPAREIPRIT
                     LRAFPALMLPVILLFGIYGGVMTPTEGAAAAAFYALFASTVLYRAVSWRQLYDAILAS
                     GKATTSIGMLIAGAMIFNYVVTIENIPASLARLMAGHAFSAITFLAVVNLILLVLGCL
                     LEGSTILLVVVPIFIPTAKALGIDLVHFGVVVVVNIMIGLLTPPYGLLLFVLANMTKQ
                     PLGVIVREAMPFIVMAILVLAMITVVPDSILWLPRLWGYKG"
     gene            complement(4820..5335)
                     /locus_tag="VFL49_04735"
     CDS             complement(4820..5335)
                     /locus_tag="VFL49_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET7911312.1"
                     /translation="MQMSQLITVARWLRRRAENIAVALLCVMFATFIVQIFARYVLNN
                     PVGWSEEVIITTWLWTVLWGAAFILRESEEIRFDIIYSKVSERTRRVFTVLTGVALIV
                     LYGISLPAAYSYVSFMKVERSAYLRVPINWLYCVFIIFSVACICRYCWLVCRAVRGSK
                     SPETDPVQLPD"
BASE COUNT         1244 a         1622 c         1674 g          959 t
ORIGIN      
        1 gcggtgctca aggcgctcgc cgatcaatgc accaaggcac tctttttccc gatcgggaag
       61 cacgccggat ggcatccgga aattctgaaa caggtggccg ccgcgggaca tacagttggc
      121 tcgcacacct ggtcgcacaa ggatctgtcc aagttgagcc agcaggaagg gatcgacgag
      181 atcgagaagg gcatcgcggc cgtcagcatc gcgctgggca acaagccgac cgctccgttc
      241 ttccgctttc cggcgctgcg gcatccgccc gagctgctga aatatgtcgg cgagcgcaat
      301 atcggcgtct tctcgactga tcttgattcc ttcgacttca agatgcgcaa gcccgaccag
      361 gtcatcgcat cggtgatgaa gaagctcgaa aagcacggca aaggcatcat cctgatgcac
      421 gactttcagc aggcgacggc ccatgcggca ccggaactgc tgaaaaagct gaaggatggc
      481 ggctacaagg tcgtgcaggt ggtcggcaag actcccctcg agccgaagaa ggaatatgtc
      541 gacatggtcc tcaaggaaat gggcggcgga atggaatcag cgcgacccat gtccagcgtc
      601 atcaaggata tcagcaacta attgaacggc gctgtttttc ccgccggccg cgcagcattg
      661 cgcggccggc ttttttctcg cgcacgccaa acccgcggct ttgtgataac ctgatcgcag
      721 cagaggtcac ggacgcgggg ggttatccat gaacgtgctg ctcatcgcct tcggcgcact
      781 tgccgcactt tgggttgttg ttctcctggg atcgcggttc tttggtccga aggagcaata
      841 gccacgcgac tcaaggcacc gcccgtcccg agaaggcggt gaagatcgac gatgcggcgg
      901 tggcgtgttc gtaggcaatc cgcgtcgcaa cgaagaacag catcgttgcg agcaccagcc
      961 ggagcgcgcg ctcgggaata cggatcgcga aatagctgcc gacgaagatg cccggcagcg
     1021 agccggcgag cagcgagccg ataatcgccc aatttacgga ccccatcatc cagtggccga
     1081 ggccggcgat caaagtcagc ggaacggcat gcgcgatgtc cgaaccgacg atcttggcca
     1141 tgggcaaatg cgggtagagc atcaccagtg cgatgacgcc catggcaccg gcgccgactg
     1201 aagaaatcga tacaagacag ccgagaacgg ccccgaccac gatcgtaatc gtcgccgtcc
     1261 tctccgggtc gagatcgaca acgcgcgcgc ggtagacgcg cacgatcgac gccccgaaaa
     1321 caagcacgaa cgcggtcgca aacaaggcta tactcaacac aagcgtgatc aggttgcgtg
     1381 cagcgtcgcc gctcagattg aaatgcgaaa gcgcagccag cgtgagaatc gtcgcgggaa
     1441 tgctcccggt cgcaagccgg ccgaccacgc gccagtcgat gctgcgggca aagccatgta
     1501 ccagactgcc gccggttttg gtcgcggcgg cataaagcag gtctgttccg accgcggtcg
     1561 cggggtggac accgaacagc atgatcagga tcggcgtcat caacgcgccg ccgccgactc
     1621 cggtcatgcc aacaagcagg ccgacggcaa agcccgacag cgagtacagc ggatcaatca
     1681 tcagaatgac ctcgtcgcga caggcctgcc gctaatctaa gcatggccaa tctcagcagt
     1741 catcgtgacg aaatggggga acgccccatg gacatggctg ttccgcacaa ttttctgaaa
     1801 tatgcggcat ggcaccgcaa cccaaaaata cccatttcgt tcggtaaaag gccgttccgc
     1861 aacgcgaatt atccactgga cgctacacgt ggctgatatt gttacgctta cgatcaaccc
     1921 cgccattgac attttcgtca atgtcggccg cgtcgagccg acacgaaaaa tgcgttgttc
     1981 ggcgccaaaa cgcgatcccg gcggcggcgg tatcaatgta gcccgcgtcg ctcaccggct
     2041 gggaggacgg gtgaccgcga tcttcccgac gggcggcgct atcggaaagc tgctgcaccg
     2101 gctcgtcgac cgggaaggca tcgacagcat cgtgacgccg tcgcacgtcg agacgcggga
     2161 aaactttacg gcctacgagg aatcgaccgg ggagcagtat cgtttcgttt tgcccggatc
     2221 gacgctgcat cgcgcggaat gggaaacggt tctcgagaag ctgacgacgc tttcgcagcg
     2281 gccgaaattt gtcgttgcaa gcggcagcgt gccacccggt gtcccggatg atttcttcgc
     2341 ccgcgtcgcc cgccacgcga agatgttggg cgcgcaatgc gtcatcgaca cctccgggcc
     2401 tgcactgaag gcggcgctgg cggaaggcgt ttcactgatc aagccgaacc tggtcgagct
     2461 gtcggacctc gtcggggcca cgcttggcaa tgacgaggtg cggctgcgtg catgccgcaa
     2521 gctgatcgag gatggacgcg tcaaaatcgt cgcgctgacg ctcggcgaag atggcgcgct
     2581 gctggtgacc gccgatcaga cttttcgcgc agagccgatg gcaatcgagc ccgtcagcac
     2641 ggtcggcgca ggcgacagtt tcgtcggcgg gctgattgct gcgctcgcct cgggcgagtc
     2701 gttggcacaa gcttttcgag ttgctgttgc cgcagcctcc gccgccgtaa tgtcgccggg
     2761 cacggagctg tgccgccacg aggacgtgca gcgactcctg ccgcaggtga aaatcagcga
     2821 gcttgcacgc gcgaccgcct gagcggctgc tgcaatctac atctggcggc gcgctttgat
     2881 tgtcgccggc tcccgcaggt cgtccttcat cgcctcaagc atgccgagcc agatgatcag
     2941 cgggaaatag agtaacagtg gcatgttttg tttctcgcgt tgcggttcgg cagggaaacg
     3001 caggagggcg aatgccagtt ccgccgatca cgccggcgtg tttcaatacc cggatacgcc
     3061 cgccgttcct cggccgccga tcatttcagc aacttcttca tggagctcgt ggcgatccga
     3121 taaccttccg ggccgaaccc ggcgatcacc gccgttgcaa ctttcgagac aaacgaatgg
     3181 tggtagtagg cttcacgctt atggatattc gaaatatgca gctcgataat cggcccttcg
     3241 aacatcttga gggcgtcaag gatcgcaatc gacgtgaaag taaatgcggc gggattgatg
     3301 atgattccgc ctcccatgtc gatcgcttcg tgaatccagt ctatcagctc ggcctcgctg
     3361 ttggactgac gaaactcaac gggatagtca ccggcttcgt cacggcaaaa tcgttcgaca
     3421 tccgcaagcg ttgccttgcc gtaaatcgac ggctcgcgtt ttccaagccg attaagattg
     3481 ggaccgttca ggatgtagat cggtttcatt gatctgtaac ctcaaccttt gtatccccaa
     3541 agccgcggta accagagaat cgagtccgga acaacggtga tcatcgcaag cacgagaatc
     3601 gccatgacaa taaaaggcat ggcctcgcga acaatcactc ccaacggttg tttggtcatg
     3661 ttggcgagca caaagagcag caatccgtag ggtggcgtca gcagtccgat catgatgttg
     3721 accaccacca cgacgccgaa atgcacaaga tcgatgccaa gggcctttgc agtcggaatg
     3781 aagatcggca ccacgacgag cagaatggtg ctgccttcga gcagacagcc cagaaccaga
     3841 agaatcaggt tgacgacggc aagaaacgta atcgccgaaa aggcgtgacc agccatgagg
     3901 cgagcgagtg acgccgggat gttctcgatc gtgaccacat agttgaagat catcgcgccg
     3961 gcgatcagca tgccgatcga cgtcgtcgct tttccgctcg ccagaatcgc gtcgtagagc
     4021 tggcgccagg acaccgcgcg gtaaaggacg gtggaggcga aaagagcgta aaaagccgca
     4081 gccgccgcgc cttcggtggg cgtcatgacg ccaccgtaaa tcccgaacag caaaatcacc
     4141 ggcaacatca gtgccgggaa ggcgcgcagg gtaatcctcg gaatttcgcg cgcgggaacc
     4201 ggcggttcga ccggatagtg acgtcgccgc gcgatgaccg agttggcaac cataaaggcg
     4261 atgccgagca tcacgccggg cacaacaccg ccaagaaaaa ggtagccgat cgaggtatcg
     4321 gagatcagcg cgtagagcac catcggaatc gatggcggga tgatggggcc gatgatggcg
     4381 gccgaggccg tgattgcccc tgcataggca accgggtaac ggccctccct cgtcatcatg
     4441 ccgataatga tgcggccgat gccgacagcg tcggcaatcg ccgaacccga catcccggcg
     4501 aaaatcatgt tggcgaccac gttgacgtgg ccaaggccgc cgcggaaacg tccgaccagg
     4561 acaaggcaga actgcagcag cctgtcggtc aggctcccga tattcatcag gtcggccgcg
     4621 aggatgaaaa gcgggattgc gagaagaacg taggagttga acagcccgtt gaggatctgc
     4681 tcggcggcgg ttccgagatc gagtcccgac agcagcagat acaggatcga gccggcgatc
     4741 atcgcgtgac cgatcggcag acccagtgcg ccaagcgcga ctatggcaac gatgcagatg
     4801 gcaaaaggcg acggcatcgt cagtccggaa gctgcacggg gtcggtctcg ggcgactttg
     4861 agccgcgcac ggcccgacag accagccagc aatagcggca gatgcaggcg acgctgaaga
     4921 tgatgaacac gcaataaagc cagttgatcg gtacccgcag atatgcggat cgctcaacct
     4981 tcatgaagct gacgtagctg taggcggcgg gaagtgagat gccgtaaaga acgatcagcg
     5041 ccacgccggt caggaccgta aagacgcgcc gcgtacgctc cgataccttg gagtagatga
     5101 tgtcgaagcg gatttcttcg gattcgcgca gtatgaacgc ggcgccccaa agcactgtcc
     5161 aaagccaggt cgtgatgatc acctcttccg accatccgac aggattgttg agcacgtaac
     5221 gggcgaagat ctggacaatg aacgtcgcaa acatcacaca caacagcgcg acggcgatat
     5281 tctcggcgcg gcgccgcagc cagcgcgcca ccgtaatcaa ttgcgacatt tgcatcagct
     5341 ttgcccgacc caatgtgcac tgatggaatg cgcggccggt tcgggaaaat cgaaccggcc
     5401 atagccccgg actggcgcgt tcgcggaagc ccgattaccg gatagcgttg atccgctcga
     5461 gcgcgccttt cggccagtct ttgccgtatc tctcgacgt
//
LOCUS       DASSRL010000230         4930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_968330, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000230 DASSRL010000000
VERSION     DASSRL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968330"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFL49_04740"
     CDS             complement(<1..267)
                     /locus_tag="VFL49_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET7911313.1"
                     /translation="MTLAFTEGARASVRRPELDKSNTAATRALLQVQQRDGHWVFELE
                     ADATIPAEYVLLRHFRGEPIDKDLERKIAAYLKRIQGAHGGWALF"
     gene            complement(264..1535)
                     /gene="hpnE"
                     /locus_tag="VFL49_04745"
     CDS             complement(264..1535)
                     /gene="hpnE"
                     /locus_tag="VFL49_04745"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HET7911314.1"
                     /translation="MRSSRSGTVHIIGAGLAGLAAALRLTQAHRAVVVHEATAYAGGR
                     CRSYYDAATDMLIDNGTHLLLSGNHAALSFVGDIGSSVGLHGPKEAEFPFIDLANGEQ
                     WTLRFGESRFPWWVFDKGRRVPQTSIADYLPIARLAWAKADKPIADVLNCSGTLYTRF
                     LEPLLLAALNIAPPQGSAKLAGVLIRETIALGGQACRPLLARDGLGPVFVQPAVSWLT
                     QHGVSVVFEDELVALKFSDGRASQLQFSRHSVELDADDAVILAVPPYVATKLVPGLTG
                     PTAFRGIVNAHFRIDPPAKLQPILGVINGTSQWIFSLPGRICVTISDGGGLFDVPRAE
                     LAQSIWNDVAAATGLPTILPPWQIVRERRATFEATPEQNARRPEPQTRWSNVLLAGDW
                     TATGLPATIESAIRSGYRAAALIGGWMEAAA"
     gene            complement(1522..2364)
                     /gene="hpnD"
                     /locus_tag="VFL49_04750"
     CDS             complement(1522..2364)
                     /gene="hpnD"
                     /locus_tag="VFL49_04750"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET7911315.1"
                     /translation="MTAQVQQPGDSRPAAARAGGSSFYLAMRILGREQREAMFEIYSF
                     CRAVDDIADGDVPGDKLTLLVGWRDNIDALYAGQVPPGLEGLAQAISRYGLLKDDFLA
                     VIDGVEMDAQADIRAPSLEQLDLYCDRVACAVGRLSVRVFGLEESDGKMLAHHLGRAL
                     QITNILRDIDEDAGVGRLYIPREALQLAGIDTVIPQSAVTDPRLGTACTFLAERARGH
                     FTKANEVMARCTRRSVRAPKIMGIVYRRMLEDMIARGWAPPRQPVHVNKSQLIWIALR
                     HAFV"
     gene            complement(2361..3239)
                     /gene="hpnC"
                     /locus_tag="VFL49_04755"
     CDS             complement(2361..3239)
                     /gene="hpnC"
                     /locus_tag="VFL49_04755"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HET7911316.1"
                     /translation="MTTSGQLRSGKGSGDENFPVASWLIKPQHRPAILAFYEFVRVAD
                     DIADHASLTSGEKVERLNGMEASLLGKSDREPEGVRLRGVLEAQRLTPRHAQDLLDAF
                     RQDATKQRYATWDELIDYCSRSAMPVGRFVLDVHGESKAVWPANDALCAALQIINHLQ
                     DCGSDYQRLNRIYIPLDAMAAAGVSVDMLSRNRASPALRECLKGLVGQTADLLRESEG
                     LAASVKDRRLGLEIAVIQTLARRLLRILARRDPLSEPVHLSKAQATGYALRGAVGAGL
                     HRLLRAVVPAHRYQVQ"
     gene            complement(3239..4387)
                     /locus_tag="VFL49_04760"
     CDS             complement(3239..4387)
                     /locus_tag="VFL49_04760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7911317.1"
                     /translation="MTATTTIAVICVAIWVYLIVARGGYWRAGIRDNTGIAAMSGAWP
                     AVAVVIPARNESECIARSVQSLLRQDYCGPLRIVVVDDDSDDGTGAVATDAAAGDGRL
                     NVLRTNGPPAGWTGKLWAVSQGIAAAEASPADYLLLTDADIEHAPDTLSWLVAQSVTG
                     RLVLASLMAKLRCVSLAERIHVPAFIYFFQMLYPFAWVARAGSSTAAAAGGCMLVRAD
                     ALASIGGIASIRGALIDDCSLGAKLKTQGLIWLGLTNRVQSIRPYPAFADIARMVSRS
                     AYSQLRYSPLALIGTTLGMAITFIAPPMLTLFASGLPRYLGLLAWAAMALSFVPTLRY
                     YRLSPIWSVALPGIALLYMIYTLQSAYQHFRRRGGQWKGRVQAGAPSV"
     gene            4564..>4930
                     /locus_tag="VFL49_04765"
     CDS             4564..>4930
                     /locus_tag="VFL49_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7911318.1"
                     /translation="MNTPAHARLPRSVDVAIVGGGNAAFCAAHAAAEHGVSVLVLERA
                     PEEESGGNSRFTAGAFRCVYDGVDDLRALMPDLTDEEVANTDFGTYTQDKFFDDMGRV
                     TEYRTDPDLCEVLITRSKDT"
BASE COUNT          948 a         1622 c         1515 g          845 t
ORIGIN      
        1 aaagagcgcc cagcccccat gcgcaccctg gatgcgcttg agataggcag cgattttccg
       61 ctcaagatcc ttgtcgatcg gctcgccacg aaaatggcga agcagcacgt attccgcggg
      121 aatggtcgcg tccgcctcca gctcgaacac ccagtgcccg tcgcgctgct ggacctgcag
      181 cagcgcccgg gtggccgcgg tgttcgattt gtcgagttcc ggccgccgca ctgacgccct
      241 cgccccctcg gtgaatgcca gcgtcatgcc gcggcctcca tccagccgcc aataagcgcg
      301 gcggcgcgat agccggaccg gatcgcgctc tcgatcgtcg ccggcagtcc tgtcgccgtc
      361 cagtccccgg ccagcagcac attgctccag cgcgtctgcg gctccggacg gcgcgcattc
      421 tgctccggtg tcgcctcgaa ggtggctcgc cgctcgcgca cgatctgcca cggcggcagt
      481 atcgtcggaa ggcctgtggc ggcggcaaca tcgttccaga tgctttgcgc gagctcggcg
      541 cgcggcacgt cgaacaggcc gcccccatcg ctgatggtca cgcagatccg cccgggcagc
      601 gaaaatatcc actggctggt cccattgatc acaccaagga tcggttgaag cttcgccggc
      661 ggatcgatgc ggaaatgcgc gttgacgatg ccgcgaaacg ccgtcggtcc ggtcaggccg
      721 ggcaccagct tggttgcgac ataaggcggc acggcaagaa tgacggcatc atcggcgtcg
      781 agctcgacgc tgtgcctgga gaattgcagc tgcgatgcgc ggccgtcgga aaacttgagc
      841 gcgacgagct cgtcttcgaa caccaccgaa acgccgtgct gagtcagcca ggaaaccgcc
      901 ggctggacaa agaccggccc gaggccgtcg cgcgccagaa gcggacggca ggcctgtccg
      961 ccaagcgcga tggtttccct gatgagcacg ccggccagct tggccgagcc ttggggaggc
     1021 gcaatattga gcgcggcgag cagcaacggc tcgagaaaac gcgtgtagag cgtgccggag
     1081 caattcagga cgtcggcaat cggcttgtcg gcttttgccc aggcaagccg cgcgatcgga
     1141 agatagtcgg caatactggt ttgcggaacg cgccgaccct tgtcgaagac ccaccatgga
     1201 aaccggcttt cgccgaaccg caacgtccat tgctctccgt tggcaagatc aatgaacgga
     1261 aattccgcct ccttcggccc atgcaggccg accgaggaac cgatatcgcc gacaaaagac
     1321 agcgccgcat ggtttcccga cagcagcaga tgcgtgccgt tgtcgatgag catgtcggtc
     1381 gcggcgtcgt agtaagagcg gcatcgcccg cccgcatagg cggtcgcttc gtgaaccaca
     1441 acggcgcgat gcgcttgcgt aagccggagg gcggcggcaa gccctgccaa tcccgctccg
     1501 ataatatgga cagtaccgga cctagacgaa cgcatgccgc aacgcgatcc atatcagctg
     1561 ggacttgttg acgtgcaccg gctggcgcgg cggcgcccag ccgcgcgcta tcatgtcctc
     1621 gagcatccgg cgatagacga tgcccattat cttgggcgcg cggacgctgc ggcgtgtgca
     1681 cctggccatg acttcgtttg ccttcgtgaa atgtccccgc gcacgctccg caagaaatgt
     1741 gcacgccgtc ccgaggcgcg ggtcggtaac ggcggattgg ggaataactg tgtcgattcc
     1801 cgccagctgc aatgcttcgc gagggatata aagacgtcca acgcccgcgt cttcgtcgat
     1861 atcacgtaat atgttcgtga tctgcagtgc ccgacccaga tggtgggcca gcattttgcc
     1921 gtcggattcc tccaggccaa agacccgcac ggacagacgt cccacggcac aggccacacg
     1981 atcgcaatag agatcgagct gctcgaggct cggcgcgcgg atgtcggcct gcgcgtccat
     2041 ttccacgccg tcgatgacgg caaggaaatc gtctttcagc agaccgtatc gcgaaatcgc
     2101 ctgcgcgagg ccttcgaggc cgggcggcac ctggccggca taaagtgcgt cgatgttgtc
     2161 gcgccacccc acaagcaaag tgagcttgtc acccggcaca tcgccatcgg cgatgtcgtc
     2221 gaccgcgcgg cagaatgaat agatctcaaa catcgcttcg cgttgctcgc ggccgagaat
     2281 gcgcatggcg agatagaacg aactgccgcc cgcgcgcgcg gccgctgggc ggctatcccc
     2341 gggttgctga acctgcgccg tcattgcacc tgatacctgt gcgccggcac gactgcgcgc
     2401 aacagccgat gcaatccagc gccgaccgcg ccgcgaagcg catagccggt ggcctgcgcc
     2461 ttgctgaggt ggactggctc gctcaaagga tcgcgccgcg cgagaatccg caacaggcgc
     2521 ctggcgagcg tctggatcac cgcaatttca agtccgaggc gccggtcttt cacgctcgcc
     2581 gccaagccct cgctctcccg caggagatcg gcggtttgcc ccaccagtcc tttgagacat
     2641 tcgcgcagcg ccgggctcgc ccgattgcgg gagagcatgt cgacgctcac gcccgctgcc
     2701 gccatcgcgt cgagagggat gtagatgcgg ttgagcctct gataatcgga cccgcaatcc
     2761 tgcaggtggt tgatgatctg cagggccgcg cacagcgcat cgttggcagg ccagaccgct
     2821 ttcgattctc cgtgcacgtc gagcacgaag cgcccgaccg gcatcgccga gcgtgagcag
     2881 taatcgatca gttcgtccca ggtcgcgtat cgctgcttcg tggcatcctg ccggaacgca
     2941 tcaagcagat cctgcgcatg gcgcggggta agtcgctgcg cttcgagaac gccgcgcagc
     3001 cgcacgcctt ccggctcgcg atccgatttg ccgagcaggc tcgcctccat tccattgagg
     3061 cgctcgacct tttcaccgct tgtcagcgag gcgtggtcgg caatgtcgtc ggcgacgcgc
     3121 acgaattcat agaacgcgag aattgccgga cgatgctgcg gcttgatcaa ccaggacgcg
     3181 acgggaaagt tctcgtcgcc cgaccctttg cccgatcgaa gttgtccgct ggtggtcatt
     3241 atacgctcgg cgcgccggct tggacgcgcc ccttccactg gccacccctg cgccgaaaat
     3301 gttgataggc ggactgcaag gtgtagatca tgtacaacaa tgcgattccc ggaagggcca
     3361 cgctccatat gggcgaaagc cgataatacc gcaacgtggg tacgaacgat aatgccatcg
     3421 ccgcccaggc cagcagccca agatatcgcg gcaaacccga ggcaaacagg gtcagcatcg
     3481 gcggcgcaat aaaggtgatc gccatgccca gagtcgtgcc gatcaacgca agcggcgaat
     3541 agcgcaattg gctgtaggcc gagcgcgaaa ccatccgtgc aatgtcggca aaagcagggt
     3601 atggccggat gctctgcacg cggttcgtca ggccgagcca gatcagccct tgggttttca
     3661 gcttggcgcc caacgagcaa tcatcgatca acgcgccacg gatgctggcg atgccgccga
     3721 tgcttgcgag tgcgtcggcg cggacgagca tgcacccgcc ggcggcggca gccgtcgatg
     3781 aaccggcgcg cgcgacccac gcgaacggat agagcatctg gaaaaaatag atgaaggccg
     3841 ggacatgaat cctttcggca agactcacgc agcgcagctt ggccatcagc gacgccagca
     3901 caagtcggcc ggtcaccgac tgcgcgacca gccatgagag cgtgtcaggc gcgtgctcga
     3961 tatcggcgtc ggtaagcagc agataatctg caggagatgc ttctgccgcg gctatgccct
     4021 ggctgacagc ccacaatttc ccggtccagc ccgccggcgg tccattcgtt ctgagcacgt
     4081 tcagccgccc gtcgccggct gcggcatccg ttgcgaccgc gccagtgccg tcatcgctgt
     4141 cgtcgtcgac gaccacgatg cgaagcggcc cgcaatagtc ctgccgcagc agcgattgca
     4201 cgctgcgcgc gatgcattca gattcgttgc gcgccggaat gaccacggca accgccggcc
     4261 acgcgcctga catggccgcg atgccggtat tgtcccgaat gccggctcgc caataaccac
     4321 cccgcgcgac gatcaggtag acccaaatcg ccacgcagat caccgcgatg gttgtggtcg
     4381 ccgtcatcgt ctttctgccc gtatcaaccg acccagcatc cacctcgatt actacattaa
     4441 gttcaacgcg tctcccaaac catttgacaa tgtgagataa ttcgacgggc tcgcaggctc
     4501 cgcgcgttga catgccggtg cgggcacata cactccggcc ctctcgatct caggtttcct
     4561 tcggtgaata cccccgcgca cgccaggcta ccccgctctg tcgacgtcgc catcgtcggc
     4621 ggcggcaatg ccgcattctg cgccgcgcat gcagccgccg agcatggcgt ttcggtgctc
     4681 gtgctcgagc gcgcaccgga ggaggaatcc ggtggtaaca gccgttttac ggccggcgcc
     4741 ttcagatgtg tctacgacgg cgtcgatgac ctgcgtgcgc tgatgccgga cctcaccgac
     4801 gaggaagtcg ccaataccga tttcggcact tacacgcagg acaaattttt tgacgacatg
     4861 ggccgcgtga cggaataccg gaccgatccc gacctgtgcg aggtcctcat cacgcgcagc
     4921 aaggacacga
//
LOCUS       DASSRL010000231         5493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_58648, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000231 DASSRL010000000
VERSION     DASSRL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58648"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1617
                     /gene="cimA"
                     /locus_tag="VFL49_04770"
     CDS             22..1617
                     /gene="cimA"
                     /locus_tag="VFL49_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET7911319.1"
                     /translation="MLKERLFLFDTTLRDGAQTNGVDFTLADKLAIARMLDELGIDYV
                     EGGYPGANQTDSELFGDDHKLDTTFTAFGMTRRPGRSASNDPGLAALLEAKADAICFV
                     AKSWDYHVKVALETTEEENLASIRDSVRAAKAKGREVLLDCEHFFDGYKANPEYALAC
                     AKAAYDEGARWVVLCDTNGGTLPDEVEKIVGEVVKVIPGDHVGIHAHNDTDQAVANSF
                     AAVRAGARQIQGTLNGLGERCGNANLVSIIPTLKLKPEFATRFDIGVSNAALKKLAHV
                     SHALDERLNRAPNRHAPYVGESAFATKAGIHASAIMKDPATYEHVAPDMIGNRRKLLV
                     SEQAGKSNILAELDRIGIKADKGDPRVGRLLEIVKEREKIGYAYEAADASFELLARRT
                     LGKVPNYFDVTQFDVNVEQRNNAKGERVTVTMAVVKVKVGEDNMISAAEGNGPVNALD
                     LALRKDLGKYQKYIAGLKLTDYRVRILNAGTEAVTRVLIESEDEKGDRWTTVGVSPNI
                     IDASFQALMDSIIFKLVKSGAPS"
     gene            1655..2029
                     /locus_tag="VFL49_04775"
     CDS             1655..2029
                     /locus_tag="VFL49_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7911320.1"
                     /translation="MIDHISIAVRDVKAAEPFYTSLLATLGMNKLREWPDAAIGYGKR
                     YPEFWINRRSDMPPVAQDSGVHICLRADSREAVNAFHTAALAAGGTSDGGPGLRKEYN
                     DRYFAAFIRDPDGNRIEAVTFV"
     gene            complement(2033..2656)
                     /locus_tag="VFL49_04780"
     CDS             complement(2033..2656)
                     /locus_tag="VFL49_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7911321.1"
                     /translation="MNRNEKVSKIRKICVYCGSGPGTDPAFVEAGRAFGATLASNGIG
                     LVFGGGSVGMMGAIAKSVLDHGGAVTGVIPEFLVAREHAMRGADNLIVTRDMHERKRK
                     MFELADAFVAMPGGIGTLEEVVEQITWVQLGRHRKPILLANIKNFWNPLRALIDHMKT
                     LEFIRGDLDFELLVADDVADILPMLQKSAAAVPEKEKEMPAVIADRL"
     gene            2767..4740
                     /locus_tag="VFL49_04785"
     CDS             2767..4740
                     /locus_tag="VFL49_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET7911322.1"
                     /translation="MSDHHSHRASESDSRVSATRGSLFGTIVHLWPYIWPSERGDLKF
                     RVIGSLLLLLAAKLATIAVPFTFKWATDALAGHGTAPVAASDWLLWAIAAPVAMTIAY
                     GGMRILMAAFTQLRDGLFAKVAMHAVRRLAYRTFVHMHELSLRFHLERKTGGLTRVLE
                     RGRNGIETIVRMVILQLSPTIVELAFIVVVLMWQFDWRYVAVISVTVALYMSYTYRAT
                     EWRIGIRRKMNDSDTDANVKAIDSLLNYETVKYFSAEEREAARYDRAMARYEDASVRS
                     YTSLAVLNAGQAVIFTVGLAAVMVMCALEIQAGSKTVGDFVLINAMMIQLYQPLNFMG
                     MVYREIKQAVTDIEIMFSILSRDPEIKDVPNAPQLKVDSGAIRFENVVFAYDPERPIL
                     KGISFEVPAGKTVAVVGPSGAGKSTISRLLFRFYDLSGGRILIDGQDIARVTQKSLRQ
                     AIGMVPQDTVLFNDTIRYNIRYGRWEATDAEVEEAARLAQIDPLIRIAPKGYETEVGE
                     RGLKLSGGEKQRVAIARTILKGPAILVLDEATSALDSHTEKEIQDALERVSRNRTTLV
                     IAHRLSTIVGADEIIVLDAGQIVERGTHYALLARNGLYASMWNRQREAEEARETLART
                     GEDQRAPNRNPPPVEDAIAGTAALLETPADAAE"
     gene            4750..5448
                     /locus_tag="VFL49_04790"
     CDS             4750..5448
                     /locus_tag="VFL49_04790"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET7911323.1"
                     /translation="MSIYDSIRAQIAPVTPEGYPFIGAFALVSLILFWLWSPLGWLGT
                     LATLWCAYFFRDPSRVTPVRDGLVVAPADGRVSQVTSAAPPRELGLGEKPLPRISIFM
                     SVFDCHVNRSPVAGTVEKIVYQPGKFFNADLDKASVDNERNAMVIAIPGGRIAVVQIA
                     GLVARRIVCFIRVGQPIGAGERFGMIRFGSRVDVYLPEGVPPLVAVGQTAIAGETVLA
                     DLRAADGGRAFRTG"
BASE COUNT         1122 a         1579 c         1668 g         1124 t
ORIGIN      
        1 attacctttg gtgagagtcc aatgctgaag gagcgcctct ttctcttcga caccacgctg
       61 cgcgatggcg cgcagacgaa cggggtcgat tttacgcttg ccgataagct ggcgatcgcg
      121 cgaatgctgg atgagcttgg catcgactac gtcgagggcg gctatccggg cgccaaccag
      181 accgattccg agttgttcgg cgacgatcac aaacttgata cgaccttcac cgcctttggc
      241 atgacgcgtc ggccaggccg ctcagcctcc aacgatcccg ggcttgcggc gctccttgaa
      301 gcaaaggcag atgccatctg ctttgtcgcc aagtcatggg actatcacgt caaggtcgcg
      361 ctggagacga ccgaagagga aaatctcgcg tcgatccgcg acagtgtgcg tgccgccaag
      421 gcgaaaggca gggaagttct gctcgactgc gagcatttct tcgatggcta taaggccaat
      481 ccagaatatg cgctcgcctg tgccaaggcc gcttatgatg agggtgcgcg atgggtcgtc
      541 ctgtgcgata cgaacggtgg cacccttccg gacgaggtcg agaagatcgt cggtgaggtg
      601 gtgaaggtta tcccaggcga tcatgtcggc attcatgccc acaatgacac tgatcaggcg
      661 gttgcgaact catttgcggc ggtgcgagcc ggtgcgcgcc agattcaggg caccctcaat
      721 ggccttggcg agcgttgcgg caatgccaac ttggtttcca tcattccgac gctgaagctc
      781 aagccggaat tcgcgacgcg cttcgatatc ggagtcagca acgccgcgct taaaaaactt
      841 gcgcacgttt cacacgcgct cgatgaacgc ctcaatcgcg cgccaaatcg ccatgccccg
      901 tacgtgggcg aaagtgcttt tgcgaccaaa gcaggcatcc acgcttctgc gatcatgaag
      961 gacccggcaa cctacgagca tgtcgcgccg gacatgatcg gaaaccgccg caaattgctg
     1021 gtgtctgaac aggcgggcaa atccaacatc ctggcggagc tcgatcgcat cggtatcaag
     1081 gccgacaagg gcgacccgcg tgttggccgg cttttggaga tcgtgaagga acgggaaaag
     1141 atcggatatg catacgaagc ggccgacgcc tcgtttgaac tgctggcgcg ccggacgctc
     1201 ggcaaggtac cgaactattt cgacgtgaca cagttcgacg tcaacgtcga gcagcgcaac
     1261 aatgccaaag gtgaacgtgt caccgtcact atggccgtgg tgaaggtgaa agtcggcgag
     1321 gacaacatga tctccgccgc ggaaggcaac ggaccggtca acgcgctaga ccttgcgctg
     1381 cgtaaggatc tgggcaagta ccagaagtac attgctggcc tgaagctcac agactatcgc
     1441 gtgcgcatcc tcaatgccgg aaccgaggcg gtcacgcggg tgctgatcga gagcgaggac
     1501 gagaagggcg accggtggac gacggtaggc gtatcgccca atatcatcga cgcatcattc
     1561 caggccttga tggattcgat catttttaag ctcgtgaaat caggcgcacc gtcgtgagat
     1621 agtgcgggct gaaccggtaa cccggcgggg cgccatgatt gatcacattt ccatcgccgt
     1681 gcgggacgtt aaagccgcgg aaccatttta tacctcgctg ctcgcgacgc tcgggatgaa
     1741 caagctgcgg gaatggcccg atgcggccat cggttacggc aaaaggtatc cggagttctg
     1801 gatcaatcgc cgttcagaca tgccccctgt cgctcaggac agcggcgtgc acatttgctt
     1861 gcgggctgac tccagggaag cggtgaatgc ctttcacacc gctgcgcttg cagcaggagg
     1921 aacctctgat ggcggacctg ggcttcgcaa agaatataat gaccgctatt tcgcagcctt
     1981 cattcgtgat cctgacggca accgcatcga agcggtgacc tttgtgtgat tcctagagtc
     2041 ggtccgcgat cacagccggc atttcttttt ctttctccgg cactgcggcc gccgatttct
     2101 gcagcatcgg caggatatcc gcgacgtcgt ctgcaaccag cagttcaaag tccagatcac
     2161 cacggatgaa ttcaagcgtt ttcatgtgat caatcagtgc gcggagcgga ttccagaaat
     2221 tcttgatgtt ggcgagcaaa attggtttgc ggtgacggcc gagttgaacc caggtgatct
     2281 gctcgactac ctcttcaagg gtgccgatgc cgcccggcat tgccacaaag gcgtccgcca
     2341 gttcgaacat cttgcgtttg cgttcatgca tgtcgcgagt gacgatcagg ttgtcggccc
     2401 cgcgcatcgc atgttcgcgc gcaacgagga attccggaat aacgccggtc accgctccgc
     2461 cgtggtcgag gaccgatttg gcaattgctc ccatcatgcc gaccgagcca ccgccaaaaa
     2521 ccagcccgat gccattgctg gcgagggtgg cgccgaatgc tcgacccgcc tccacaaagg
     2581 cagggtcggt gccgggacca gagccgcaat aaacgcagat tttccggatt ttgctgactt
     2641 tttcgtttct gttcatgtct atgcatgtgc atcgcgcttg cggcatcgtc aaggcggcca
     2701 aaaaacaaag gtccggcctt ggcgaaggat gattacaaac gagaaatctc ctatatgaag
     2761 gccggaatga gcgaccacca ttctcatcgc gcatcggaaa gcgactcgcg tgtttccgcc
     2821 acgcgtggat cgctgttcgg caccatagtc catttgtggc cctatatctg gccttcagag
     2881 cgaggcgatc tcaaatttcg ggtgatcggc tcgctgctcc tgttgctggc ggcaaagctc
     2941 gcgaccattg cggtaccctt cacattcaaa tgggccacag acgcactcgc cggtcacggg
     3001 acggctcccg ttgccgcctc tgactggttg ctttgggcca tcgctgcgcc ggtcgccatg
     3061 actattgcgt atggcggcat gcgtattttg atggcggcgt tcacgcaatt acgcgatggg
     3121 ctgtttgcca aggttgcgat gcacgccgtg cggcggcttg cgtaccgcac tttcgttcac
     3181 atgcacgagc tgtcactgcg ctttcatctc gagcgcaaga ctggcggact gacacgtgta
     3241 ctcgaacgtg gccgcaacgg catcgaaaca atcgtacgca tggtcatcct gcaactgtcg
     3301 ccgacgatcg tcgagcttgc gttcatcgtt gtcgtgctga tgtggcaatt cgactggcgc
     3361 tatgtagccg tcatttcggt caccgtcgca ctgtacatga gctacacgta tcgcgcgaca
     3421 gaatggcgta tcggcatccg tcgaaaaatg aatgacagcg ataccgatgc caacgtgaaa
     3481 gcgatcgact cgctgcttaa ctatgaaacc gtaaagtatt tttccgctga ggagcgtgag
     3541 gccgcccgct acgaccgcgc catggcgcga tacgaagatg cgagcgtgcg ctcatacacc
     3601 tcgctcgccg ttctcaacgc cggtcaagcc gtgatcttca ccgtcgggct ggccgccgtc
     3661 atggtgatgt gcgcgctgga gatccaggcg ggcagcaaga cggtcggcga tttcgtgctg
     3721 atcaacgcca tgatgattca gctttaccag cccctcaact tcatgggcat ggtctatcgc
     3781 gagatcaagc aggcggtcac cgacatcgag atcatgttct cgatcctttc tcgcgacccg
     3841 gaaatcaaag acgtgcccaa tgcaccgcaa ctgaaggtcg atagcggggc aattcgcttc
     3901 gagaatgtgg tcttcgccta tgatccggaa cggccgatcc ttaagggaat cagctttgaa
     3961 gtgccggccg gcaaaacggt ggccgtcgtc ggtccctccg gcgccggaaa atcgacgatc
     4021 tcgcggttgc tgttccgctt ctacgatctg tcgggcggcc gcattctgat cgacggtcag
     4081 gatatcgcga gggtgacgca gaaatcgctt cgtcaggcaa tcggcatggt gccgcaggac
     4141 actgtgctgt tcaacgatac gatccgctac aacatccgct acggtcgctg ggaagcgacg
     4201 gacgcggaag tggaggaggc ggcgcgactt gctcagattg atccgctaat tcggattgcg
     4261 cccaagggct atgagaccga agtgggcgag cgcggcctca agttgtctgg cggagagaag
     4321 cagcgtgttg cgatcgcgcg caccatcttg aagggaccgg cgatcctggt attggacgaa
     4381 gcaacctcgg cgctggacag ccataccgaa aaggaaattc aggatgcgct cgagcgcgtg
     4441 tcgcgcaaca ggacgacgct cgtgatcgcc caccggctgt cgaccattgt cggggcggat
     4501 gagatcatcg tgttggacgc gggacagatc gtcgaacgcg gcacgcacta cgccctgctt
     4561 gcccgaaacg ggctatacgc gagcatgtgg aaccgtcagc gcgaggccga ggaagcgcgc
     4621 gagacgctgg cgcgaacggg tgaggatcag cgggcgccca atcgcaatcc tccgccggta
     4681 gaagatgcaa ttgccggcac cgccgctctc cttgagacgc cggccgacgc ggcggagtaa
     4741 aatagccgta tgtcgatcta cgattcgatt cgcgcgcaaa ttgcgccggt cacgccggaa
     4801 ggttatccgt tcatcggcgc ctttgcgctc gtgagcctta tcctgttctg gctgtggagt
     4861 cccttgggat ggctcggcac tctggccacg ctctggtgcg cttacttttt tcgggatccg
     4921 tcgcgcgtga cgcccgtgcg cgacggtctg gtggtggcgc ctgccgatgg ccgcgtgagc
     4981 caggtgacga gcgcggcgcc accgcgcgag ctcggcctcg gtgagaagcc gctaccgcgg
     5041 atttcgatct tcatgagcgt atttgattgc cacgtaaacc gcagcccggt cgccggaacc
     5101 gtcgagaaaa ttgtctacca gccgggaaag ttttttaacg ccgacctcga caaggccagt
     5161 gtcgataacg agcgtaatgc aatggtgatc gccatacccg gagggcgcat tgcggtcgtg
     5221 cagatcgctg gtctcgtcgc tcgtcgtatc gtctgtttca tacgtgtggg acagccaatc
     5281 ggggcaggag agcgttttgg catgatccgc ttcggttccc gtgtcgatgt ctatcttccg
     5341 gaaggcgtgc cgccccttgt cgcggtgggt caaaccgcaa ttgccggaga aacggtgctt
     5401 gccgatctgc gcgcggccga cgggggccgt gcgttccgca cggggtaaac gattaacgag
     5461 accgggaaaa tgcacctcgg gcgccgtggc gtc
//
LOCUS       DASSRL010000232         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_41244, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000232 DASSRL010000000
VERSION     DASSRL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41244"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFL49_04795"
     CDS             complement(<1..394)
                     /locus_tag="VFL49_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET7911324.1"
                     /translation="MSTQPDLRGVQRAVQGALHRHWVLFLIEGIVLLVLGVLAVLVPP
                     VATLAVTILFGWLFLISGIVGLVTTLWMRNAPGFWWSLISAVLGIVVGLLLLARPLGG
                     ALSLTLLLIAFFIVEGAVSIMFALDHKRE"
     gene            complement(467..1438)
                     /gene="msrP"
                     /locus_tag="VFL49_04800"
     CDS             complement(467..1438)
                     /gene="msrP"
                     /locus_tag="VFL49_04800"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HET7911325.1"
                     /translation="MNVICRRGWDISDRLATPEHLAFSRRSFLAGGASALAMTPYAAQ
                     AQRVSDVAKLPDPSESLYPAKRNEKYVLDRPITDEKVNGNYNNFYEFGTSKTIAAAAQ
                     ALPIRPWTVKIDGMVEKPMEVGIDDLIHKLTLEERTYRHRCVEAWGMAIAWTGFPFAK
                     LVAFARPLGSAKYVRMETFMNPKIAPGQKQPWFPWPYIEGLTMAEATNELAFLATGAY
                     GHALARQHGAPLRLAVPWKYGFKSIKSIVRFTFTDTRPKGLWEALQASEYGFWANVNP
                     DVPHPRWSQASEEIIGTGERRPTLLFNGYGEYVAGLYKGLEKERLWA"
     gene            complement(1550..>2506)
                     /locus_tag="VFL49_04805"
     CDS             complement(1550..>2506)
                     /locus_tag="VFL49_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7911326.1"
                     /translation="WRQCLAVNRPAAALTQNSRHARMRRRIRCADTGMLQGFRNLFSS
                     GPVPYADPKSSIPLGAVLRHGWMELWYQPKVELRSTHLVGAEALVRARHPTRGIIPPA
                     LFLPGASDQDMHELTERVILTALGDWEDFAEYSTPLKLAVNVPVSALVHLPIPRMLRE
                     ARPRASSWPGLILEVTEDQIINDLGIANDVAETLRELKCELAVDDFGAGYSSLVRLKQ
                     LPFSELKIDRSYVANCNEDHFNAGLCETIVELGRRFGLRTVAEGIETTRESHKLQAIG
                     CQIGQGYLFAKPMPKADFLSRLRARVKGAGISQAMSGRVALS"
BASE COUNT          496 a          785 c          763 g          462 t
ORIGIN      
        1 attcgcgctt gtgatcgagc gcaaacatga tggagacggc gccttcgacg atgaagaacg
       61 cgatcagcag cagcgtgagg gaaagcgcgc cgcccagagg ccgcgccaac agcagaaggc
      121 cgacgacgat cccgagcact gccgaaatca gcgaccacca gaacccgggc gcgttgcgca
      181 tccataatgt cgtgacaagg ccgacgatgc cgctgatgag gaacagccag ccgaacagga
      241 tggtgaccgc aagggttgca accggcggca caagcaccgc caacacgccg agaaccagca
      301 gcacgatgcc ttcgatcagg aacagcaccc agtgcctgtg caacgcgcct tggaccgcgc
      361 gctggacgcc tctcaggtcg ggttgcgtgg acatgcgagc ctccgcgttt tttcccgtcg
      421 cggctggtat actcccgctc gcgctttcgg tacagcaccg ctcggctcac gcccaaagac
      481 gttctttttc cagccccttg tagaggccgg cgacgtactc gccatagccg ttgaaaagca
      541 gcgtcggccg ccgctcgccg gtgccgatga tttcttcgct cgcctggctc cagcgcggat
      601 gcggcacgtc cggattgacg tttgcccaaa agccgtattc cgacgcctgc aacgcctccc
      661 acaggccctt ggggcgtgtg tcggtgaaag taaaccgcac gatcgacttg attgacttga
      721 acccgtattt ccaaggcacc gccagccgca gcggcgcgcc gtgctggcgc gcaagcgcat
      781 ggccataagc gcccgtcgca agaaatgcga gttcgttggt cgcctccgcc atcgtgaggc
      841 cttcgatata aggccacgga aaccagggct gcttctgacc cggggcgatc ttcggattca
      901 tgaaggtttc catccgcacg tattttgcgg agccgagcgg gcgcgcaaac gcaacgagtt
      961 ttgcaaacgg gaagccggtc catgcgatcg ccatccccca ggcctccacg caccgatgac
     1021 ggtaggtccg ctcttcgagc gtcagcttgt gaatgaggtc gtcaatgccg acttccattg
     1081 gcttctcgac catgccgtcg atcttcaccg tccacggcct gatcggcagc gcctgcgctg
     1141 ccgcggcgat cgtcttcgag gtgccgaatt cgtagaaatt attgtagttg ccgttgacct
     1201 tctcgtcggt aatcggccgg tcaagcacat atttctcgtt gcgcttggcg ggataaaggc
     1261 tctcgctcgg atcaggcaat ttggcgacgt cggaaacgcg ctgggcctgc gccgcatagg
     1321 gcgtcatcgc caacgcgctg gcgccaccgg ccagaaaact gcggcggcta aaagccagat
     1381 gctcaggtgt cgcgagccga tcggatatgt cccagccgcg gcggcagatg acgttcatcg
     1441 caaggtcctc cagaatcgca tatccagagg taaaagccgg ccggctcact ggcaagtcac
     1501 gcttctgcga gaggaaaccc cgccgtcagg ccggatctaa tggcgtgatt cagctgagcg
     1561 cgacccggcc ggacatcgcc tgcgatatcc cggctccttt cacccgcgca cgcaggcggc
     1621 tgagaaaatc tgctttcggc attggctttg caaacaggta gccctgcccg atctggcagc
     1681 cgatcgcctg cagtttgtgg ctttcgcgcg tggtttcgat gccttctgca acggtgcgca
     1741 ggccgaaacg gcggccgagc tcgacgatcg tctcgcacag gccggcattg aagtgatcct
     1801 cattgcagtt ggcgacatag ctgcgatcga tcttcagctc gctgaacggc agctgcttga
     1861 ggcgaaccag cgacgaatag ccggcgccga aatcgtccac cgccagttcg cacttgagtt
     1921 cgcgtaacgt ctccgcgacg tcattggcaa taccgagatc gttgatgatc tgatcttcgg
     1981 tgacttcaag gatgagaccc ggccagctcg atgcgcgggg acgtgcctcg cgcaacatgc
     2041 gtggaatcgg cagatgaacc agcgccgaga ccggaacgtt cactgcaagc ttgagcggtg
     2101 tgctgtattc ggcgaagtct tcccaatcgc ccagcgcggt gagaattacg cgctcggtca
     2161 gttcgtgcat gtcctgatca ctggcgccgg gcagaaacag cgcgggtgga atgatgccgc
     2221 gtgtcggatg gcgcgcgcgt acgagcgcct cggcgccgac gagatgcgtt gagcgtaact
     2281 cgaccttcgg ctgataccac agctccatcc agccgtgccg taacaccgcg ccgagcggaa
     2341 tagacgattt tgggtcggca taaggcaccg gcccggatga aaacagattt cgaaagccct
     2401 gcagcatgcc cgtatccgca cagcgaatgc gccgcctcat tcgcgcatgg cgggaatttt
     2461 gcgtgagggc ggcggctggc cggttaacgg cgagacactg ccgcca
//
LOCUS       DASSRL010000233         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1598212, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000233 DASSRL010000000
VERSION     DASSRL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598212"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFL49_04810"
     CDS             complement(<1..410)
                     /locus_tag="VFL49_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7911327.1"
                     /translation="MASNEKNYFDPKVLAGISNLALRARWVVEGLMSGIHRSRAKGFS
                     VEFEEHREYSPGDEIRRIDWKALGKFDRYFIKEYEDETNLRAYLLLDASASMNYASDG
                     ISKFDYACTLTASLAYLILKQQDAVGVVTFSDRI"
     gene            complement(495..1034)
                     /locus_tag="VFL49_04815"
     CDS             complement(495..1034)
                     /locus_tag="VFL49_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911328.1"
                     /translation="MQWEICHGDLRLPFRPRRYIVGHGIELYGDAEFCRRTQEALALL
                     RPLALFEIICAHLGIIRQGKRSGMKAWAVKPTFVVGKPTWSHSALWYAGAVAHDAYHA
                     VLYAEAKKRDGGKEPPAATWTGAAAEKQCLAFQREVLVELNADEKLIGYIDRCARDPT
                     YQGHHQGWRGWLDYLKRRW"
     gene            complement(1072..2100)
                     /locus_tag="VFL49_04820"
     CDS             complement(1072..2100)
                     /locus_tag="VFL49_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7911329.1"
                     /translation="MEATEIQQHEIAEIEEFAVKRDSMLGEIRKVIVGQDQVIEEVLI
                     ALFARGHCLLVGVPGLAKTLLISTLAEILDLDFNRVQFTPDLMPSDITGTDILQEDAG
                     TGRRSFQFLKGPIFTNILLADEINRTPPKTQAALLQSMQEYNVTAGGSTFPLDLPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMVNIEIGYPNFEDEVQIVMQTTSTGKPAPRKIMDG
                     PAILRYQELVRKVPVSPFVASYAVALAQRSRPQSPDASPYVKDYVEWGAGPRASQYLI
                     LGAKARTILQGRYAVSIEDIQALAPSVLRHRIVPNFKAQGEGLSSIDIIHRLLSDVKP
                     SADGKATK"
     gene            complement(2120..>2613)
                     /locus_tag="VFL49_04825"
     CDS             complement(2120..>2613)
                     /locus_tag="VFL49_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7911330.1"
                     /translation="VRKLKVKKDQREDEEVVELREIQSAVARNRTHLADQLLGKGRTE
                     AAANEYQRALQASPYSPIILNKLGRIMVETHRPDDALPLFKKALDVDPDNVNTYVQLG
                     RAQVAKKNFKEARVALEEAIQINPFNPLIYRLLGDTYAALGDQEKARSAKATLDQLMT
                     SRN"
BASE COUNT          481 a          744 c          809 g          579 t
ORIGIN      
        1 tcgatgcgat cggagaaggt cacgactccg accgcgtcct gctgcttgag aatgagatag
       61 gcgagcgacg cggtgagcgt gcaggcgtaa tcgaatttgc tgatgccgtc cgaggcatag
      121 ttcatcgacg cgctggcatc gagcaggaga taagcacgca ggttggtttc gtcttcgtat
      181 tctttaatga agtagcggtc gaacttgccg agcgccttcc agtcgatgcg gcggatttca
      241 tcgccgggcg aatattcgcg gtgctcttcg aattcgacgc taaagccttt ggcgcggctg
      301 cggtggatgc cggacatcaa tccttcgacc acccagcggg cacgcagcgc gaggttggaa
      361 atgcccgcaa ggacctttgg gtcgaagtaa tttttctcgt tgctagccat gctttttaac
      421 ggccgaaggt cgagagtaga gtagcagatc gaagatcgcg attctccatc cgcgatcctc
      481 catcctcgga gtttttacca ccggcgtttc agataatcta gccagccgcg ccaaccctgg
      541 tgatgtcctt gataggttgg atcgcgcgcg cagcgatcga tgtaaccgat gagcttctcg
      601 tcggcgttga gctcaaccag cacctcgcgc tggaaggcga gacactgctt ttccgccgcc
      661 gcacccgtcc aagtcgcggc gggcggttcc ttgccgccgt cacgtttctt agcctccgcg
      721 tacagcacgg catgataggc gtcgtgagcg acggcaccgg cataccagag ggcggaatgg
      781 ctccaggtcg gcttgccaac gacgaaagtg ggttttaccg cccaggcttt catgccgctc
      841 cgtttgccct ggcggatgat gccgagatgc gcgcaaatga tttcaaatag cgccagcggc
      901 cggagcagag ccaatgcttc ctgggtgcgg cggcaaaact ccgcatcgcc gtaaagctcg
      961 atgccgtggc cgacgatgta tcggcgcggc cggaacggca gccgcaaatc cccatgacag
     1021 atttcccatt gcatggcgaa ttcgaaatct gcgcgccggc aatcgcaaat tctatttcgt
     1081 cgctttgccg tcggcggacg gttttacgtc gctgagcagg cgatggataa tgtcgatgga
     1141 cgagagtcct tcgccttgcg ccttgaagtt cggcacgatg cggtggcgca gcacggaagg
     1201 ggcgagggct tgaatgtctt cgatggaaac cgcgtagcgg ccttggagaa tcgtacgcgc
     1261 cttggcgccg agaatcagat attgcgaggc gcgcggtccg gcgccccact cgacgtaatc
     1321 cttgacgtac ggcgaagcgt ccgggctttg cggccgcgag cgctgcgcca gcgccaccgc
     1381 gtaggaagct acgaacggag agaccggaac tttccgcacc agctcctggt agcgcaggat
     1441 cgccggaccg tccatgatct tgcgcggcgc cggcttgccg gtcgacgttg tctgcatgac
     1501 gatctgcact tcatcctcga agttcgggta accgatctcg atgttgacca tgaaccggtc
     1561 gagctgcgct tcgggcagcg ggtaggtgcc ttcctgctcg atggggtttt gggttgccag
     1621 gacaaagaac ggcaggtcga ggggaaaggt gctgccgccg gcggtgacgt tgtattcctg
     1681 catcgactgc agcagcgccg cctgcgtctt gggcggcgtc cggttgattt catcggccag
     1741 caaaatattc gtaaagatcg gtcccttgag aaactgaaaa ctgcgccggc cggtgccggc
     1801 atcttcctgc agaatgtcgg tgccggtgat atcggagggc atcagatcgg gcgtgaactg
     1861 gacgcgattg aaatcgagat ccaaaatttc cgcgagggtg ctgatcagca gggttttggc
     1921 caatcccggc acgccgacca gtaggcaatg tcctctggcg aacagcgcga tcagaacttc
     1981 ctcgatgacc tgatcctgac cgacgatgac tttgcgaatc tcgccgagca tgctgtcgcg
     2041 tttgaccgcg aactcttcga tctcggcgat ctcatgctgc tgtatctcgg ttgcttccat
     2101 gatacccctt ctcgatggat taatttcggc tcgtcatgag ttgatcgagc gtggccttgg
     2161 ccgaccgggc cttttcctgg tcgccgaggg cggcgtacgt gtcgccgagc agccggtaga
     2221 tgagcgggtt gaacgggttg atctggatgg cttcctcgag cgcaacgcgc gcctctttaa
     2281 agtttttctt ggcaacctgc gcgcgaccca attgcacata agtattgacg ttatcggggt
     2341 cgacgtcgag cgccttcttg aatagcggca gagcgtcgtc gggacgatgg gtttcgacca
     2401 tgatgcgtcc gagtttgttc aggatgatcg gcgagtacgg gcttgcttgc agggcgcgct
     2461 gatattcgtt ggccgcggct tcggtgcgcc ccttgccgag caactggtcg gcaagatgcg
     2521 tccggttgcg cgccaccgcc gattggatct ctctcagctc cacgacttct tcatcttcgc
     2581 gctgatcttt tttcaccttc agcttgcgca ccc
//
LOCUS       DASSRL010000234         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_968372, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000234 DASSRL010000000
VERSION     DASSRL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968372"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VFL49_04830"
     CDS             complement(<1..1159)
                     /locus_tag="VFL49_04830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7911331.1"
                     /translation="MSEPGKFAVSGSVPAEAPADGYRVLARKYRPASFDDLIGQDAMV
                     RTVSNAFESGRIPQAWILTGVRGVGKTTTARILARALNFELPDGSVKEPTIRMPVMGV
                     HCQAIIESRHPDVLEMDAASHNSVDDVRQINDAIRYAPMSARYKVYILDEVHMLSGAA
                     FNALLKTLEEPPPHAKFIFATTEVRKVPVTVLSRCQRFDLRRVEASVLVEHLQGIAAR
                     ERVDVEPAALALIARAAEGSVRDSLSLLDQAIAHAAGPVRAEDVRQMLGLADRVRVVD
                     LFEALMKGDASAALKELRDQYETGADPAVILSDLADFTHFVTRVKVVPAVADDVSLSE
                     AERTRGRTFAAQLSMRVLSRAWQMLLKGISDVQASGRPIAAAEMVLVRIAYA"
     gene            complement(1221..1316)
                     /gene="ffs"
                     /locus_tag="VFL49_04835"
     ncRNA           complement(1221..1316)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFL49_04835"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1410..1859
                     /locus_tag="VFL49_04840"
     CDS             1410..1859
                     /locus_tag="VFL49_04840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET7911332.1"
                     /translation="MDSGPAKGWSLHPQLAHDTVPVGDLPLARALLTNDANYPWLILV
                     PRLPGLVELIDLEENAQVQLLGEIAATARALKSITQCDKLNIAALGNQVPQLHVHIIA
                     RRHDDAAWPKPVWGAKPPLPYEQAKLHALLETLRRLLKPEGLIAMDR"
     gene            1883..>2137
                     /locus_tag="VFL49_04845"
     CDS             1883..>2137
                     /locus_tag="VFL49_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911333.1"
                     /translation="MAANPSRILIVSLRRIGDLLLTTPVIRSVRRAWADSQIDVLVFE
                     GTAGIIAGNPDIDRVLTMAQRPTAGESLGLIRRLWRSYDIA"
BASE COUNT          422 a          690 c          631 g          394 t
ORIGIN      
        1 cggcgtaggc gatgcggaca agcaccatct cggcagcagc gatgggacga ccggatgcct
       61 gcacgtccga aattcccttg agcagcattt gccaggcgcg cgacagcacg cgcatcgaca
      121 gctgcgccgc aaaagtgcgc ccgcgcgtgc gctccgcctc cgacagagac acatcgtcag
      181 cgaccgccgg aacgactttg acgcgcgtaa caaaatgggt gaagtcggcg agatcactca
      241 ggatcaccgc cggatcggca ccggtttcat attgatcgcg cagctctttc agagctgcag
      301 aggcatcgcc tttcatgagc gcttcgaaaa gatcgaccac gcgcacgcga tcagccaagc
      361 cgagcatctg acggacgtct tcggcgcgaa ccggaccggc cgcatgcgct attgcctgat
      421 ccaacagcga gagcgaatca cgtacggagc cttccgctgc gcgcgcgatc aacgcaagcg
      481 ccgctggttc cacgtccact ctctctctgg cggcaatgcc ctgcaaatgt tccaccagga
      541 cagatgcctc gacgcggcgc agatcgaaac gctgacagcg ggacaacacc gttaccggca
      601 ccttgcggac ctcggtggtc gcgaaaataa atttcgcgtg cggcggcggc tcttccagcg
      661 tcttgagcag cgcgttgaag gccgcacccg acagcatgtg tacttcgtcg agaatgtaga
      721 ctttgtagcg cgcgctcatc ggcgcgtagc ggatcgcatc attgatctgc cgcacgtcgt
      781 ccacgctgtt gtgtgaggcg gcatccattt ccagcacgtc gggatgccgg ctctcgatga
      841 tggcctggca atgcacgccc atcaccggca tcctgatcgt tggctctttc accgagccat
      901 ccggcagttc aaagttgagg gcacgtgcga gaatgcgtgc ggtcgtcgtt ttcccgacgc
      961 cgcgtacacc ggtcagaatc caggcctgcg gaatacggcc gctctcgaac gcgttcgata
     1021 ccgtccgaac catggcgtcc tgaccgatga gatcgtcaaa acttgccggg cggtatttgc
     1081 gtgcaagcac gcggtatccg tcagccggcg cctcggccgg gacggaaccg gaaacagcga
     1141 atttgccggg ctcgctcatc gtgccatccg cttcgccgcc gcgcgacgcg cggactgatt
     1201 gcgaacaact ctaggaatta gtgggaggct gacgagcgac ccgaaccgcg ctcgttaggg
     1261 ctgcttcctt ccggacctga cccggttggc gagtggctcg tccaccgcca acctcccggg
     1321 gtctatatcg ggccaataaa ccgggaaagc aagcagcgcc acagcgtctc tgctagcttt
     1381 cctccgaaag aatcgcgtgg atttatccaa tggattcagg acctgccaag ggctggtcgc
     1441 tgcacccgca gctcgcgcac gacacggttc ccgtcggcga cctgccgctg gcccgcgcgt
     1501 tgctgacgaa cgacgccaac tatccgtggc tgatccttgt cccgcgcctg cctggactcg
     1561 ttgaactcat cgaccttgaa gagaatgcac aagttcagct gctcggtgaa atcgccgcaa
     1621 cggcgcgcgc gctgaaaagc atcacccaat gcgacaagct taatatcgcc gcgctcggaa
     1681 atcaggtgcc gcaattgcat gtgcatatta tcgcgcgtcg ccatgacgat gccgcctggc
     1741 caaaaccggt ctggggcgca aaaccgccgc ttccctacga acaagcaaag ctgcacgcgc
     1801 ttctggagac gctgcggcgg ttgctcaagc ccgaaggtct catcgccatg gaccggtagt
     1861 tccggttctg tcaaaaatcg gcatggccgc caatccctcc cgcattctga tcgtatcgtt
     1921 gcgccggatc ggcgatctgc tgctgacgac gccggtgatc cgcagtgtgc gccgcgcctg
     1981 ggcggattcg cagatcgacg tgctggtctt cgagggcacc gcaggaatca ttgccggaaa
     2041 tccggatata gatcgtgttc tgactatggc tcaacggccg accgcagggg aaagcctcgg
     2101 cctgatccgg cgcctgtggc gctcctacga cattgcg
//
LOCUS       DASSRL010000235         6266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1318457, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000235 DASSRL010000000
VERSION     DASSRL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318457"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFL49_04850"
     CDS             complement(<1..417)
                     /locus_tag="VFL49_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911334.1"
                     /translation="MVIADPLALIRGAHFAATLLASGTVCFAALTIGSTRLQSYPVLR
                     MRMNVLVWSGLALAFITGAMWLVWLASNILGESLADVGLHGGVLSVLAETRFGWIWCL
                     RLALAVLVALPVLDPAWRVIRVVAAVVFLVSPAFVGH"
     gene            complement(439..807)
                     /gene="copC"
                     /locus_tag="VFL49_04855"
     CDS             complement(439..807)
                     /gene="copC"
                     /locus_tag="VFL49_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HET7911335.1"
                     /translation="MAIHRSVAAALGAALTLVPIAALAHAHLDHASPAAGSTVAESPK
                     EVSLWFTEALEAKFSAIEVRDAQGAAVQAGPAALARDNTAQLRVPLKPLKPGTYKVIW
                     RVLSVDTHRTQGNFTFRVGP"
     gene            complement(810..1349)
                     /locus_tag="VFL49_04860"
     CDS             complement(810..1349)
                     /locus_tag="VFL49_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016737106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HET7911336.1"
                     /translation="MFRKPWPLAILAQAFGLAITFTPSTVLAHVTLEQQQAPVGTPYK
                     VVLRVPHGCSESATIRLTVQIPEGVIAVKPMVKPGWQIATSRGPYAKAYSYLHGAKFT
                     EGIREVRWDGGKLPDAFYDEFVLSTFIAGELSPGRMLYFPVVQQCEQGEHRWVEIPAA
                     GKHPNEPAPGVMLTPKKQE"
     gene            complement(1411..1788)
                     /locus_tag="VFL49_04865"
     CDS             complement(1411..1788)
                     /locus_tag="VFL49_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911337.1"
                     /translation="MPTGFRRALSILAIAAVALHTALWGATAISMEVAPIDPFSVICH
                     SPGNVDAAAAQAPAGPLSKPSHACDHCNLCGSMPPPAASPAAVVGELLPDRLLHELEP
                     VSSVARAGSEVALKGARGPPGFA"
     gene            1935..3257
                     /locus_tag="VFL49_04870"
     CDS             1935..3257
                     /locus_tag="VFL49_04870"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7911338.1"
                     /translation="MRLSRYFLPILRETPKEAEIVSHRLMLRAGMMRQEAAGIYAFLP
                     LGFRVLQKICRIVREEQDRSGAIELLMPIIQSADLWRESGRYDAYGKEMLRIKDRHER
                     DMLYGPTNEEMITDIFRAYVRSYKDVPLNLYHIQWKFRDETRPRFGLMRGREFLMKDA
                     YSFDLDQAGATHSYNKMFVAYLRTFARMGLKSIPMRAESGPIGGNLSHEFIILAQTGE
                     SEVFCHKNYLDMAVPPADVNFDDVPGLQAIVNRWTSQYAATSDMHDAAAFAKVPAGKQ
                     VSARGIEVGHIFYFGTKYSEPMKAMVATHDGKEVPVHMGSYGIGPSRLVAAVIEASHD
                     DAGIIWPEPVAPFKVVILNLKQGAAETDGACEQLYRDLTAKGVEVLYHDLDERPGAKF
                     ATADLIGIPYQVLVGPRGLAEGKVEVKKRADGSRENISPAAALDLLAK"
     gene            3430..4698
                     /locus_tag="VFL49_04875"
     CDS             3430..4698
                     /locus_tag="VFL49_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET7911339.1"
                     /translation="MTEANGTRPFSPFEWMLSLRYLRARRKEGFISVIAGFSFLGIML
                     GVATLIIVMAVMNGFRKELLDKILGLNGHLLIQPLEKPLTDWEEVAARVAGVKGIRLA
                     APIVEGQALASSPFNAGGVLIRGIRLRDLEKLPSIANNIKQGTLKGFDEDMGVVIGRR
                     LADQLSLRSGDNITLVAPRGAVTPMGTTPRIKVYKIAAVFEIGMSEYDSAMVFMPLKE
                     AQAYFNRAGDVTAIEIYTDNPDRVDRYRKPVQDAAQRPVFMIDWRQRNATFFNALQVE
                     RNVMFLILTLIVLVAALNIVSGLIMLVKDKGQDIAILRTMGATQGAIMRVFLITGASI
                     GVIGTIVGFLLGIVICLNIESIRQFLSWLTNTELFSPELYFLSHLPAQMDSSETTAVV
                     VMALVLSLLATLYPSWRAARLDPVEALRYE"
     gene            4724..5449
                     /locus_tag="VFL49_04880"
     CDS             4724..5449
                     /locus_tag="VFL49_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911340.1"
                     /translation="MVEEQQQPPQARQEEDIPVLFLHDVERQYRQGDETLHVLNGVDL
                     ALWPGQSVALVAPSGAGKSTLLHIAGLLEHPDHGDVYIDGRSTSILADHERTRIRRNE
                     IGFVYQSHHLLPEFSALENVMLPQMIRGLARTEAKARASELLEYLGLAGRLNHRPAEL
                     SGGEQQRVAIARAVANAPRVLLADEPTGNLDAHTAEHVFRALSQLVTASGLAAVIATH
                     NMDLAARMDRRVTLREGRIIEMD"
     gene            5853..>6266
                     /locus_tag="VFL49_04885"
     CDS             5853..>6266
                     /locus_tag="VFL49_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7911341.1"
                     /translation="MPGLDFVHLHVHSSYSLLEGALTISRLAELAKADRQPALALTDT
                     DNMFGALEFSEKISGYGIQPIVGCALAIDFGDSDHGPRNPNAQPERTRIVLLAARPEG
                     YRSLMRLNSRAFLDTPANEPPRLKVEWLQEETAGVI"
BASE COUNT         1230 a         1864 c         1956 g         1216 t
ORIGIN      
        1 gtgcccaaca aaggcaggcg aaacgagaaa gacgaccgcc gcgacgaccc gaatgacgcg
       61 ccacgccgga tcgaggaccg gaagcgcaac aaggacagcg agcgccagcc gcaaacacca
      121 gatccatccg aagcgcgtct cggcgaggac ggacaacacg cctccgtgca ggccgacgtc
      181 ggcgagagac tcgccaagga tgttggaagc aagccagaca agccacatcg cgccagtaat
      241 gaacgccagg gcaagcccgc tccacaccag cacgttcatg cgcatgcgca ggaccggata
      301 actctgcaga cgggtcgatc cgatagtcaa tgccgcaaag cacaccgttc ccgacgccag
      361 caacgtcgcg gcaaaatgcg cgccacgaat gagagcgagc ggatcggcga tcaccatgcg
      421 gcctcgacgc gacgactcct aagggccgac gcgaaatgtg aagtttccct gtgtgcgatg
      481 cgtgtcgacc gaaagtacgc gccagatcac cttgtaggta ccgggcttaa gtggcttgag
      541 cggcacacgg agttgggccg tgttgtcgcg ggcaagcgca gcaggtcctg cttgcacagc
      601 cgcgccttgc gcgtcgcgca cttcgatcgc gctgaatttc gcctcgagcg cctcggtgaa
      661 ccagagggag acttctttcg gcgattccgc caccgtgctg ccggccgcag ggctcgcatg
      721 atcgagatgt gcgtgggcca gcgcggcaat cgggacgagc gtgagggctg cacctaaagc
      781 agcggcgacc gatcgatgga tagccatggc tactcctgct tcttcggtgt cagcataacg
      841 cccggcgccg gctcgttggg atgtttgccg gcggccggga tttcgaccca gcgatgctcg
      901 ccttgctcgc actgctgcac caccggaaaa tacaacatcc gtcccggcga cagctcgccg
      961 gcgatgaaag tcgacagtac gaattcgtcg tagaaggcgt caggcagctt gccgccatcc
     1021 catctcacct ccctgatgcc ttcggtaaat tttgcaccgt gtaaatagct gtaggccttt
     1081 gcgtatggcc cgcgcgatgt tgcgatctgc cagccgggtt tcaccatcgg tttgaccgcg
     1141 ataactcctt cgggaatctg gaccgtgaga cgaatggttg cggattcgct gcatccgtgc
     1201 ggtacgcgca ggacgacttt gtacggtgtg ccgacaggcg cctgctgttg ttcgagggtg
     1261 acatgcgcga ggacggttga tggcgtaaag gttatggcaa gtccgaatgc ttgcgccaga
     1321 atggccaaag gccatggttt tctgaacatg ggtcgtctcc agtgtcgcat tgacggttga
     1381 actcgccggc gatgccggct gcaaatgacg tcaggcgaag cctggaggac cgcgggcgcc
     1441 tttcaacgcg acctcagacc cggcacgtgc aacgctcgat accggttcga gctcatgcaa
     1501 cagccggtcc ggaagaagct caccgacgac ggcagcggga gaggccgcgg gcggtggcat
     1561 cgagccacag aggttgcaat ggtcgcaggc gtggctgggc ttcgagagcg ggcctgccgg
     1621 ggcctgcgcg gccgccgcgt cgacgtttcc cgggctgtga cagatcaccg aaaatgggtc
     1681 gatcggcgcg acttccatgg agattgccgt tgcgccccac aatgcggtgt gcagggcgac
     1741 ggcggcgatc gccaaaatgc tcagggcccg tctgaagcct gtaggcatgc tctcctcgcg
     1801 acgctgcggc ggtgtagcag acaatagatg cagcgtgcgc cgataaattc ccgaagacct
     1861 gcctcggccc ttgtgttttt acggccgatc cgctttcact ctgacgctac cacctcgaat
     1921 cttccaagga ttccatgcgc ctgtcccgct atttcctgcc catcctgcgc gagacgccga
     1981 aagaggcgga gatcgtgtcg caccgcttga tgctgcgcgc cgggatgatg cgccaggagg
     2041 ccgcgggtat ctacgccttc cttccgctgg gtttccgcgt gctgcagaag atctgccgga
     2101 tcgtgcgcga agagcaggac cggtccggag cgatcgagct tttgatgccg atcatccagt
     2161 cggctgatct gtggcgcgaa agcgggcgtt acgacgccta cggcaaggag atgctgcgca
     2221 tcaaggaccg gcatgagcgt gacatgctct acggcccgac caacgaggaa atgatcacgg
     2281 acatcttccg cgcctatgta cgttcctaca aggatgtccc gctcaatctt tatcacatcc
     2341 aatggaaatt ccgcgacgag acgcgcccgc gcttcggcct gatgcgcggc cgcgaatttc
     2401 tgatgaagga tgcctattca ttcgatctcg atcaggccgg agcaacgcat tcctacaaca
     2461 agatgtttgt ggcctacctg cgtacgttcg cgcgcatggg gctgaaatcg attccgatgc
     2521 gggcagagtc ggggccgatc ggtggaaatc tctcgcatga gttcatcatc cttgcgcaga
     2581 ccggtgagtc ggaggtgttc tgccataaga actatctcga catggcggtg ccgccggcgg
     2641 atgtgaattt cgatgacgtc ccgggactgc aggcgatcgt gaacagatgg acgtcacaat
     2701 atgccgcaac ctcggacatg cacgacgcgg cggcttttgc gaaggtgccg gccggcaagc
     2761 aggtttcggc ccgtggtatc gaggtaggtc atattttcta tttcggcacc aaatattccg
     2821 agccgatgaa ggcgatggtt gcaacgcatg acggcaagga agtgcccgtg catatgggtt
     2881 catacggcat tgggccgagc cgtctggtgg cggccgtcat agaagcgtca cacgacgatg
     2941 ccggcatcat ctggccggag ccggttgcgc cgttcaaggt tgtgatcctg aaccttaagc
     3001 agggggcggc ggagaccgac ggcgcctgcg agcagctcta ccgcgatcta acggcgaaag
     3061 gggtcgaggt gctctatcac gacctcgatg agcggccggg cgccaaattc gcgaccgcgg
     3121 accttatcgg aattccgtat caggtcctgg tggggccgcg cgggctggcc gaaggcaagg
     3181 tcgaggtcaa aaagcgtgcc gacgggagcc gcgaaaacat aagtcccgcg gcggcactag
     3241 atttgttggc gaaatagcga ccggtaagtg gcgaatagag gcggctcgag aacgaccgac
     3301 tatttgctac gctcaattcg ctcttcgccg ccttaaaatc ctgtgtgttg acaagggcct
     3361 tcgctgtctt gccgggcacg aatccgccag aattcgcgtt aacagtctcg cgtggggaat
     3421 cctgcatcaa tgaccgaggc caacgggacg cggcccttct ccccgttcga atggatgttg
     3481 tcgctgcggt acctgcgtgc gcggcgcaag gaaggattca tttcggtcat cgccgggttt
     3541 tctttcctag gaattatgct gggcgtcgcc accctgatca tcgtgatggc ggtgatgaat
     3601 ggctttcgca aggaactgct cgacaagatt ctcggtctca acggtcatct gctgatccag
     3661 cctttggaga agcctttgac cgactgggaa gaggtggcgg cccgcgttgc cggcgtcaaa
     3721 gggatcaggc ttgccgcgcc gatcgtcgaa ggccaggcgc tggcatcctc gccgttcaat
     3781 gccggcggcg tgctgatccg cggcatccgg ctcagggatc tggagaagct tccctcgatc
     3841 gccaacaaca tcaagcaggg cacgctcaag ggcttcgacg aggacatggg cgtcgtcatc
     3901 ggaaggcgcc tcgccgatca gctgtcgctt cgatcgggag ataacattac gctggtcgcg
     3961 ccgcgcggcg cggtcacgcc gatgggcacg acgccgcgca tcaaggttta caagatcgcc
     4021 gcggtgttcg agatcggcat gtcggaatac gacagcgcga tggtcttcat gccgttgaaa
     4081 gaggcgcagg cctatttcaa tcgcgccggc gacgtcacgg ccatcgagat ctatacggac
     4141 aacccggacc gcgtcgaccg ttatcgaaag ccggtgcagg atgccgcgca gcgtcccgtc
     4201 ttcatgatcg actggcgtca gcgcaacgcg acctttttca acgcgctgca agtcgaacgc
     4261 aatgtaatgt tcctgatcct gaccttgatc gtcctcgtcg ccgcgctcaa tatcgtgtcc
     4321 ggtctgatca tgctcgtgaa ggacaagggc caggacatcg cgattctgcg cacgatgggg
     4381 gcgacgcagg gagcgatcat gcgggtattt ctcattaccg gcgcttcgat cggggtgatc
     4441 ggaacgatcg tgggattcct tctggggatc gtcatctgcc ttaacatcga gagcatccgg
     4501 caattcctgt cctggctgac caacacagaa ctgttttcgc ctgaactgta cttcctctcg
     4561 catctgccgg cgcagatgga ttccagcgag accacggcag tggtggtgat ggcgctcgtg
     4621 ctgtccttgc ttgccacgct ctatccgtcg tggcgcgcgg cgcgtctcga tcctgtggag
     4681 gcgctgcgct atgagtgaag cttacaccat tgccaggacg gaaatggtcg aggagcagca
     4741 acagccgccg caggcccggc aggaagagga tattccggtc ctcttcctgc acgacgtcga
     4801 acgccaatat cggcagggtg atgagacgct ccatgtgctc aacggcgtcg atcttgcgct
     4861 ctggccgggt cagtcagtgg cgctggttgc cccgtccggg gccggcaaat cgacgctttt
     4921 gcatatcgcc ggactgcttg agcaccccga tcacggcgac gtctatatcg atggccgctc
     4981 gacgtcgatc cttgccgacc acgagcggac ccgaatccgc cgcaacgaga tcggcttcgt
     5041 ctaccagtcg caccatctgc tgccggaatt ctccgcgctg gaaaatgtaa tgctgccgca
     5101 gatgatccgt ggcctggcgc ggaccgaggc caaggcgagg gcgtccgaac tgctcgaata
     5161 ccttggattg gccggccgtt tgaatcaccg gccggcggag ctttcggggg gcgagcaaca
     5221 gcgcgtcgca attgcgcgcg cggtcgcgaa cgccccgcgt gttcttctcg ccgacgagcc
     5281 gacgggaaat ctcgatgccc acaccgccga gcacgttttc cgcgcgctgt cgcaactcgt
     5341 gaccgcatca ggactcgccg cggtcattgc cacccacaac atggatctgg cggcccgcat
     5401 ggaccggcgg gtgacgttgc gagagggcag gatcatcgag atggactgac cgccatggcg
     5461 agtatttcgc ctgaggataa cctcataacg atatgattta acaagatatt attgctggaa
     5521 gcggtccctc ttgactcaag aacaaacact gaactatgtt cgctctctgt tctgggagga
     5581 catcatgttg aaaaccgtcg ttcaggaagc cgcggcgctg gcctcgctgt cactgtttgt
     5641 cggaatgatc gcgatctgga ctcaggttct cgggaatctc tagccttcat tctcgaccgt
     5701 cattccgggt tcgcggcctg aaggccagga acccggaatg accagtgatg cgaaaagcgg
     5761 gcatagcggg gtttgcggcg gcattcccgg tggacgcggg cgccgagtgt cctcactctg
     5821 cggcatagaa ggaatcagcc aaagtcacgg acatgcccgg cctcgatttc gtgcacttgc
     5881 acgtgcactc gtcctattcg ctgctggaag gggcgcttac gatttcgcgg ttggccgagc
     5941 tcgcaaaagc cgaccgccag ccggccctcg ctctgaccga caccgacaat atgttcggtg
     6001 cgctggagtt ctcggaaaaa atttcgggtt acgggatcca gccgatcgtc ggctgtgcgc
     6061 ttgcgatcga ttttggtgac tccgaccacg gtccgcgcaa tccgaatgcc cagccggaac
     6121 gtacgcgcat tgtgctgctg gctgcgcggc ccgaaggcta ccgtagcctt atgcgcctca
     6181 attcgcgcgc atttctggac acgccagcca atgagccgcc gcgcctcaag gtcgaatggc
     6241 tgcaggaaga gactgccggc gtcatt
//
LOCUS       DASSRL010000236         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_985920, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000236 DASSRL010000000
VERSION     DASSRL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985920"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..931
                     /locus_tag="VFL49_04890"
     CDS             44..931
                     /locus_tag="VFL49_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7911342.1"
                     /translation="MRFDFTDLNLFRHIVEAGSITHGAARSNLALAAASTRVRKMELA
                     FGTPLLVRGRAGIKPTPAGRTLLQHARGILAQTERLQEDLSPYARGLAGQVRVLSNTN
                     ALTEFLPETLSSFLMAYPDISVDLEERLSDEIVGLIAEGHADIGIVAGTVDYGGLNTF
                     PFRSDRFVLVVAKNHPLANRSRIGFAEVLDYDMVGLDRASAIQRFLADKANRIGRPLR
                     LRVQLRSFDAICRLVECNVGVGIVPETTVQRTERTMSISGIRLTDPWALRELTICVRD
                     VDVLPPYARQLVDHLRASA"
     gene            complement(961..2019)
                     /locus_tag="VFL49_04895"
     CDS             complement(961..2019)
                     /locus_tag="VFL49_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7911343.1"
                     /translation="MSAPSKTAKGNFFEDFHLGQKIRHATPRTVTVGDVALYNGLFGT
                     RFAVQSSDMFAKKIGYPRAPLDDLLVFHVVFGKTVADISLNAVANLGYADCRFLRPVF
                     PGATLTAVSEVIGLRENSNKKTGIVYVRSRGFDEMGETVLDFARWVMVRKRDENAPTP
                     AENIPQLPDAVDAKQLGGACPVLNLVEYDNELAGSPSRFGDYKTGDRIDHVDGITVEE
                     AEHQIATRLYQNSARIHFNQFAESKGRFGRRLIYGGHAISLARALSFNGLANAFHVAA
                     INGGRHVAPLFAGGTVFAWTEVLATAEIPKRKDVGALRLRTIATNDRPCPDFPLKGGG
                     DYDPAVILDLDYWALIPR"
     gene            2191..2418
                     /locus_tag="VFL49_04900"
     CDS             2191..2418
                     /locus_tag="VFL49_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /protein_id="HET7911344.1"
                     /translation="MSAAIAVGLILSPLAPVAFADDMKKDTMSKDSMSKDSMKKDSMA
                     RDSMSKDSMSKDSMKKDSMSKDNMSRDGMKK"
BASE COUNT          512 a          729 c          762 g          506 t
ORIGIN      
        1 tttatgtgta gtctaccttc gccaaataag aaggtagttc cctatgcgct tcgatttcac
       61 cgatttgaat ttgttccggc acatcgtcga agccggcagc atcacgcatg gtgcggcgcg
      121 ctcgaacctg gccttggccg cggcctcgac ccgcgtccgc aagatggaac tggcgttcgg
      181 tacccctctg ctggtgcgtg gccgagcggg gatcaaaccc acccccgcgg gacgtacgct
      241 gctgcagcac gcgcgtggga tcctggcgca aaccgaacgg ttgcaggagg acctctcgcc
      301 ttatgccagg ggtctcgctg gccaggttcg cgtgctctcg aatacgaacg ccctcaccga
      361 attccttccc gaaacgctga gctcgttcct gatggcctat ccggacatca gcgtggatct
      421 ggaagaacgg ttgagcgatg agatcgtcgg tctgattgca gaaggccacg ccgacatcgg
      481 aatcgtcgcc ggcacggtcg actacggcgg gctcaatact tttccgttcc gaagcgaccg
      541 cttcgtgctg gtggtcgcaa agaatcatcc actcgccaat cgctcgcgca tcggctttgc
      601 cgaagtgctc gactacgaca tggtaggcct ggatcgcgcc agcgcgatcc agcgcttttt
      661 ggccgacaag gcaaatcgca tcggccggcc gttgcgcctg cgcgtgcagt tgcgcagctt
      721 cgatgccatc tgccggctgg tcgaatgcaa tgtcggtgtc ggtattgttc ccgaaacgac
      781 cgtgcaacgc accgagcgca caatgtcgat cagtggcatc cggctcaccg atccctgggc
      841 cttgcgtgaa ttgaccattt gtgtgcgtga cgttgacgtg ttgccgcctt atgcacgaca
      901 gctcgtcgat catttgcgtg cgagtgcctg acgcaggtcc ctgcgtgcaa tccttcgcat
      961 ctatcttgga atcagcgccc agtagtccag gtcaaggatg actgccggat cgtagtcgcc
     1021 gccgcctttg agcggaaagt cgggacatgg cctgtcgttg gttgcgatcg tgcgcaaccg
     1081 caaagcgccg acatctttcc gctttggtat ttcggcagtg gccagcacct cggtccacgc
     1141 gaagactgtg ccgccggcaa acagcggcgc gacgtgcctg ccgccgttga ttgcagccac
     1201 atggaatgcg tttgcgaggc cgttgaatga cagtgcgcgc gcgagcgaga tggcgtgacc
     1261 gccgtagatc agccggcgac cgaaacggcc cttgctctcg gcgaactgat tgaaatggat
     1321 gcgggccgaa ttctgataga gccgcgtcgc aatctggtgc tcggcctcct cgacggttat
     1381 gccgtcgacg tgatcaatcc ggtcgccggt cttgtagtcg ccgaaacggg aaggactgcc
     1441 cgcgagttcg ttatcgtatt cgaccaagtt gagcaccggg caggcgccgc caagctgctt
     1501 cgcatctaca gcatcgggaa gttgcgggat gttctctgcc ggggtcggtg cgttttcgtc
     1561 gcgctttcgc accataaccc accgcgcgaa gtcgagcacg gtttcgccca tttcgtcgaa
     1621 gccgcgcgag cgcacataga cgatgccggt tttcttgttg gaattttccc tcaatccgat
     1681 gacttcggaa acggcagtca gtgtggctcc tggaaatacc ggcctgagaa aacggcaatc
     1741 ggcgtagccg agatttgcga cggcgttgag cgagatgtcg gccacggtct tgccgaaaac
     1801 gacgtgaaaa accagcaaat cgtcgagcgg cgcgcgcgga tagccgattt tcttcgcgaa
     1861 catgtcggag gattgcaccg caaaacgcgt gccaaacagc ccgttgtaga gggctacatc
     1921 gccgacagtg acggtgcgcg gggtggcgtg ccggatcttc tggccgaggt gaaaatcctc
     1981 gaagaagttg cctttggcgg tcttggatgg cgcactcaca aatcgactcc tgatcgggcc
     2041 cggtaacgct tgaccattct cacaaaagcg tgaggcgaac gccagcttgc ggcaacggga
     2101 aactgatgag gacgtgttca gcctgtgtgg tagcgaagaa gtgcaacgca aattacccct
     2161 ggaggacgtc tcgtgaaaaa gagcactctt atgtcggcgg cgatcgcggt cggcctgatc
     2221 ctttccccgc ttgcaccggt cgcctttgcc gacgacatga aaaaggacac catgtccaag
     2281 gacagcatgt cgaaggattc gatgaagaaa gactcgatgg ccagggacag catgtcgaag
     2341 gacagcatgt ccaaggactc gatgaagaag gactctatgt cgaaggacaa catgtccaga
     2401 gacggcatga aaaaataacc ggtcaaagcc gggccggcgc atccgccggc cccgctgcgc
     2461 cgtacattcg tcgaaagcga gggacgacga tgagcgcaga agccatatc
//
LOCUS       DASSRL010000237         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1668351, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000237 DASSRL010000000
VERSION     DASSRL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668351"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFL49_04905"
     CDS             <1..392
                     /locus_tag="VFL49_04905"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET7911345.1"
                     /translation="IAARPESARNRLISLIESHLSPLIDRSNFVKVFLNERQHLPRES
                     RRRIGRWSRGLERIFEDVIKEGVAKGEFRADLDVRLATLAILGMCNAASSWQRKENID
                     VSDIAEEFARLVTDGMGKRPTARRKRR"
     gene            479..2356
                     /locus_tag="VFL49_04910"
     CDS             479..2356
                     /locus_tag="VFL49_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7911346.1"
                     /translation="MTVMVDRDRLRRTTAPHLLCERARTMPDSVAFRSKHFGVYRERR
                     WRDYAGLVAHAARALQELGIVRGERVAIMGDVCEEWMICDLAAQSLGAITYGIYPTAA
                     PAEVEYQMRDGGACVFIAEDQEYVDKILPIADRLPDLRAIVVLDDSAMFGYAHEKLHR
                     FADLLPAVQKPDLAWLEKKLAGLSPADPAFIVYTSGTTGPPKGALVSHGKHLAATDTV
                     VAHYPTLREKPHRTVVYLPMCHVLGRDVAITLPLMSQLVPHFGEDPEDFTATLFEVAP
                     TVMFTVPRYLQKFASHVLVGVLNSSRIKRSAYELAVQFARKHVRRRWDGTASGLHEAA
                     YRALRTLIFRPILNKLGFDRLELVVSGGASLPAETAVLWQIYGVNVVEMYGQTEEAGG
                     IITGQRGPFARPGNVGTAVDGVEVSLAADGEVLIRSPDLFDCYWQNDEATREIKRADG
                     WLRTGDIGEWRDGALRLVDRARDFIVTVGGKTISPSFIENALRASPYVSEAVVFGHGR
                     KYLTAVIEIDFDTVADWARTNNVGYTGFTSLTTHPQIEKLMRAEIDRVNGDLARVEQI
                     KSFRILPKALDPEEEGEPVTPTRKVKRKLMYERFRDLVDGMYDDSEERLIAAGAGDVK
                     F"
BASE COUNT          442 a          762 c          767 g          438 t
ORIGIN      
        1 cgattgccgc ccggccggaa agcgcacgaa accggctgat ttcccttatc gagtcccacc
       61 tttcaccgct gatagaccga agcaatttcg tgaaggtgtt cctcaatgag cgccagcatc
      121 tgccgcgcga aagccgccgc cgcatcggcc gctggtcgcg cgggctcgag cgcatcttcg
      181 aggacgtcat caaggagggc gtcgccaaag gcgaattccg cgccgatctc gatgttcgcc
      241 tggcgacact tgccatactc ggcatgtgca atgcggcatc gagttggcag cgcaaggaaa
      301 atatcgacgt atcggatatt gccgaggaat tcgcgcggct ggtgaccgac gggatgggca
      361 agcgccccac ggcgcggcgc aagcgccgct gaaggccggt cttgcttgac gcccgcccgg
      421 atatggaatt cgatagcgct atagaatttt gccggaccaa gcgcggaaag agcgcccgat
      481 gaccgttatg gtcgatcgcg acaggctgcg ccgcaccacc gctccgcacc tgttgtgcga
      541 gcgggcgcga acgatgccgg attcggttgc cttccgctcc aagcatttcg gcgtctaccg
      601 cgagcgccgc tggcgcgact acgcgggact tgtcgcgcat gcggcgcgcg ctctgcagga
      661 acttggtatt gtccgcggcg agcgcgttgc catcatgggc gacgtctgcg aagagtggat
      721 gatctgcgat ctggccgccc aatcgcttgg cgccatcacc tacggaatct acccgaccgc
      781 agcgccggcc gaggttgagt accagatgcg cgacggcggc gcctgcgtct tcatcgcgga
      841 agaccaggaa tatgtcgaca agatcctgcc gattgccgac cggctgcccg acttgcgcgc
      901 gatcgtcgtg ctcgacgatt cggcaatgtt cggctacgcg cacgagaagc tgcatcgctt
      961 cgcggatttg ctgcccgcag tacaaaagcc cgacctcgcc tggcttgaaa agaagctggc
     1021 cggtctttcg ccggccgacc cggccttcat tgtctacacg tcgggcacga cgggccctcc
     1081 caaaggcgca ttggtcagcc acggcaagca tctggccgcc accgataccg tggtcgcgca
     1141 ttatccgacc ttgcgcgaga agccgcatcg caccgttgtt tatctgccga tgtgccatgt
     1201 gctcggccgc gacgtcgcca tcacgctgcc gctgatgtcg cagctggtgc cgcatttcgg
     1261 tgaggatccg gaggacttca cagccacatt gttcgaggtc gcgccgacgg tgatgttcac
     1321 tgtgccgcgc tatctgcaga agttcgcctc gcatgtcctg gtcggcgtgc tcaattcgag
     1381 ccgcatcaaa cgcagcgcct acgaattggc cgtgcagttc gcgcgcaagc atgtccgtcg
     1441 ccgctgggac gggacggcgt cgggcctgca tgaggccgcc taccgggcgc tgcggaccct
     1501 gattttccgc ccaattctca acaagcttgg cttcgaccgg ctggaactcg tggtcagcgg
     1561 cggcgcttcg ttgccggcgg agaccgccgt cctctggcag atctacgggg tcaacgtcgt
     1621 tgaaatgtac gggcagaccg aggaggccgg cgggatcatc accggccagc gcgggccctt
     1681 cgcgcgaccc ggcaatgtcg gcaccgctgt tgacggcgtc gaggtctcgc ttgccgctga
     1741 tggggaagtg ctgatccgca gcccggattt gttcgactgc tactggcaaa acgacgaagc
     1801 gacgcgcgag atcaagcgtg ccgacggctg gctgcgcacg ggcgatatcg gcgaatggcg
     1861 cgacggcgcc ttgcggcttg tcgaccgcgc gcgcgatttc atcgtcaccg tcggcggcaa
     1921 gacgatttcg ccgtccttca ttgagaacgc gctgcgcgcg agtccttacg tgtcggaagc
     1981 cgtggtgttc gggcatggcc gcaaatacct gacggcggtc atcgagatcg atttcgacac
     2041 cgtcgccgac tgggcgcgca ccaacaatgt cggctatacc ggattcacca gcctcaccac
     2101 ccatccgcag atcgaaaaac tcatgcgcgc cgagatcgac cgcgtcaacg gcgatcttgc
     2161 gcgcgtcgag cagatcaagt cgtttcgcat cctgccgaag gcgctcgatc cggaagagga
     2221 gggtgagccg gtgacgccga cgcgcaaggt caagcgcaag ctcatgtacg agcgcttccg
     2281 cgatctcgtc gacggaatgt acgacgacag cgaggagcgg ctgatcgccg ccggcgccgg
     2341 agatgtgaag ttctagagac agaaacaatg acaacgggag ggaccaccat gttcaggaag
     2401 gctttgctt
//
LOCUS       DASSRL010000238         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1423703, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000238 DASSRL010000000
VERSION     DASSRL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423703"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFL49_04915"
     CDS             <1..212
                     /locus_tag="VFL49_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HET7911347.1"
                     /translation="HLGEGHIAYVKQIRSEDVPGLFPQAPNLAPGMKLFALHAADGTP
                     IMLTDSREAAIANAWSNELQAVSVH"
     gene            complement(381..620)
                     /locus_tag="VFL49_04920"
     CDS             complement(381..620)
                     /locus_tag="VFL49_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911348.1"
                     /translation="MSTHVNKVHHVTTITQVAKDLGEDEDWLWDIANEMEIEDGVIWV
                     YGVGEDGVQAFTDFGIENLIELIRFYKENPGLLKR"
     gene            complement(617..2266)
                     /locus_tag="VFL49_04925"
     CDS             complement(617..2266)
                     /locus_tag="VFL49_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET7911349.1"
                     /translation="MDADRDAAPDDIAALKEALAVERAKGLQIAAELAVARAKASEDE
                     ALIAQQKLQIAKLRHQIYGQRSERSSRLIEQLALTFEELEADATEDELAAERAVAKTT
                     TVRGFTRKRAERQTFPEHLPRERVVIEPPAACECCGSSRLRKLGEDVTRTLEVVPRQW
                     KVIETVREKFSCRDCEKISQVPAPFHAIPRGWAGPSLLAMVMFEKFGQHQPLNRQAER
                     YALEGVPIALSTMADAVGSVCAALDPLLRLVEAHVMAAERLHADDTTVPVLAKGKTDT
                     ARCWIYVRDDRPFGGAGPPAAMFYYSRDRKGEHPQGHLARYTGILQADAYDGYNQLYL
                     AGRQPGPIREAACWSHGRRPFFVMADIEENARRKAAGKKEIPLSPIAVEVVRRIDALF
                     EIDRSINGRSAEERVRIRQTLSRPLVEDLQIYMREQLVKLSRGHDLAKAFNYILKRWA
                     SFTLFLEDGRVCLSNNAAERGLRGIALGRKSWLFCGSDRGGRRAAAMYSLIVTAKMNG
                     IDPQAWLTDILARIAAHPAHRLDELLPWNWTPASEISARAA"
     gene            complement(2340..2687)
                     /gene="tnpB"
                     /locus_tag="VFL49_04930"
     CDS             complement(2340..2687)
                     /gene="tnpB"
                     /locus_tag="VFL49_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET7911350.1"
                     /translation="MIPIPSGVRVWIATGHTDMRRGMQSLALAVQESLKRDPHAGDLY
                     IFRGRRGDLVKILWHDGLGMSLYAKRLDRGKFIWPSASDGAVSISAAQMAYMLEGIDW
                     RNPQLSWRPQSAG"
     gene            complement(2921..3076)
                     /locus_tag="VFL49_04935"
                     /pseudo
     CDS             complement(2921..3076)
                     /locus_tag="VFL49_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          563 a         1052 c          985 g          632 t
ORIGIN      
        1 ctcatctcgg cgaaggccac atcgcttatg tgaagcagat ccgctccgag gacgtaccag
       61 ggctgttccc gcaggcgccg aatcttgcgc cgggaatgaa gcttttcgcg ctccacgcgg
      121 ctgatggcac gccgatcatg ctgaccgatt cgcgcgaagc cgcgatcgcg aatgcctgga
      181 gcaacgagct gcaggcggta agcgtgcatt agggcacgct agcctttaat cgcgcatgac
      241 gagtttaatg cgcgggtatt ggcctcgatg atcgaggtcg atacccgcgc atttttgctt
      301 gtcgactttg tttgttcgag gcgttgcgtc tatcgtcacg ctgatgcgtg cgttgtatcc
      361 atgcggcgta ggccgcgggg ttaccgcttg agcagtccgg ggttctcttt gtagaatctg
      421 atcagctcga tcaggttttc aattccgaag tcagtgaagg cctgcacgcc gtcttctccg
      481 acgccataga cccagatgac gccgtcctcg atctccattt cgttggcgat gtcccagagc
      541 caatcctcgt cttcgccgag gtctttggcg acctgggtga tggtggtgac gtgatggacc
      601 ttgttgacgt gcgtgctcat gccgctcgag cggatatctc tgatgccggc gtccaattcc
      661 agggcagaag ttcgtccagt cgatgagccg gatgggcggc gatgcgggcg aggatatccg
      721 taagccacgc ctgcgggtcg atgccattca tctttgctgt gacgatcaag ctgtacatgg
      781 cagcggcgcg ccgcccgccg cggtcagatc cgcagaacag ccaggatttt cgtccaaggg
      841 cgatgcctct taggcctcgt tcggcggcat tgttggagag acacacgcgt ccatcttcca
      901 ggaacagcgt gaagctcgcc catcgcttca ggatgtagtt gaacgccttg gccaggtcat
      961 gtccgcggga cagcttgacg agctgttcgc gcatatagat ctgaaggtcc tcgaccagag
     1021 gccggctcag cgtctgccga atccgaacgc gttcctcggc actcctgcca ttgatggagc
     1081 ggtcgatctc gaacagcgca tcgatccgcc gcaccacctc gaccgcgatg ggcgagagcg
     1141 ggatttcctt tttgcccgcg gccttgcgcc gggcattttc ttcgatatcg gccatgacaa
     1201 agaatgggcg ccttccatgc gaccagcagg ctgcctcccg gatcggcccg ggctggcgcc
     1261 cggccagata gagctggttg tacccgtcat aggcgtccgc ctgcaggatg ccggtatatc
     1321 gggccagatg cccctgggga tgctcgccct tgcggtcgcg ggagtagtag aacatcgctg
     1381 ctggcggccc cgcaccccca aacggccggt cgtcccggac atagatccag caccgtgcgg
     1441 tgtcggtctt gcccttggcc aggactggca cggtcgtatc atcggcatgg aggcgctcgg
     1501 ctgccatgac atgggcttcc accaggcgca gcagtggatc cagcgccgca cagaccgatc
     1561 ccacggcatc cgccatggtc gacagcgcga tcggcacgcc ttccagggca tagcgctcag
     1621 cctggcggtt caagggctga tgctggccga acttctcgaa catgaccatg gccaagaggc
     1681 tcgggccggc ccatccccgg gggatggcgt ggaacggcgc cggcacctgg ctgatcttct
     1741 cgcagtcgcg gcaggagaac ttctcccgca ccgtctcgat caccttccac tggcgcggca
     1801 ccacttccag cgtgcgtgtc acgtcctcgc cgagcttacg caggcgactg ctgccgcagc
     1861 actcgcaagc cgccggcggc tcgattacca cccgctcgcg tgggaggtgc tcggggaagg
     1921 tctggcgctc ggcgcgcttg cgcgtaaatc cgcgtaccgt cgtcgtcttg gccacggccc
     1981 gttccgccgc aagctcatcc tcggtggcgt cggcttccag ctcttcgaac gtcagcgcca
     2041 actgctcgat caggcgcgaa gaacgctccg accgctgtcc gtagatctga tgccttagct
     2101 tggcgatctg cagcttctgc tgggcgatca gcgcctcgtc ttccgacgct ttcgcgcggg
     2161 caaccgcgag ttcagcggcg atctgcagac cctttgcgcg ctcgacagcc agcgcctcct
     2221 tcagggcggc aatgtcatcc ggagcagcgt cgcggtcagc atccatgcga cgcactgaat
     2281 cacagattgg acacgttggg attccccaaa atgccagcag ccgcagaatt ttttctggat
     2341 cagcccgcgc tctgcggtcg ccagctcagt tgcggattcc gccagtcgat tccttccagc
     2401 atataggcca tctgcgccgc cgagattgat accgcgccgt ccgacgctga gggccagatg
     2461 aacttgccgc ggtccagacg tttggcgtag agcgacatgc ctagcccgtc atgccagagg
     2521 atcttgacca gatcgccgcg gcggccccgg aagatgtaga gatcgccagc atgaggatcg
     2581 cgcttaaggc tctcctggac cgcaagcgcc aggctttgca tgccgcggcg catgtcggtg
     2641 tggccagtgg cgatccaaac cctgactccg ctcggaatcg ggatcatcgc cgtttcagaa
     2701 gctcaagaac tcgctgcagc gcctcaacgt ccacgtcgcg gtcaacgcga acgcgacagc
     2761 cgccaagctc gatctcgatg attccagccc ttgcacgctg cgcagacagt ggagacgctg
     2821 gttttggcac gccgttcgag atcggagccg ccacaggcgt gatctcgatg ggaacaagag
     2881 caggcgcggc atctccgccg agtcgaccct ggcgcgcttg tcgacgccac gtgaacagca
     2941 ggctatgagc caccccgtgc cggcgcgcga cggcgcagac cgtctcgcca gcctgcaagg
     3001 tctcttcgac caggcgaacc ttctcgtcgt aactccatcg ccgccggcgc tcggcgccca
     3061 gcacattgac ctgcattcgc cacgtgacct taaagctaga cttaaggtca aatcctcggc
     3121 ccgcctctca gattacacaa ggcggccgcc gccggagcga tacccttggc cggcttgccg
     3181 ccttggaact ggccgcacgt tggcgccgca atggccgcaa accgtgcaga cg
//
LOCUS       DASSRL010000239         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1546034, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000239 DASSRL010000000
VERSION     DASSRL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546034"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1543
                     /locus_tag="VFL49_04940"
     CDS             278..1543
                     /locus_tag="VFL49_04940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7911351.1"
                     /translation="MLLAGGLQSSTVDLPTLSLVAVCIAGLLGLFLIIDWMQQRNVRA
                     LAWWGSAYLIGASAMALGTMPAPFIKLPAALPGALTFLACGMVWNGVRLFQGRRVLPF
                     ATFIGAVLWLGLAQIPGMMDRGNGEVLGALIVPVYTFFIAMELWRERRRTRYSRAAAI
                     VVPCLHAGIFLVPLAMRALLPDGHSGAWLTVFTLETILYAVGTAFIVMLMVKDHHVHV
                     YRTAASTDHLTGLLNRRAFIENAATLCKRQARRGEPVAVLMFDLDHFKSINDRFGHSV
                     GDEVLRLFARVTRASTRLNDIIGRFGGEEFIAIVPGGIEIARKIAERVRTAFENAGTV
                     IESQPIGATVSIGAVSSYDATMPVETLIARADAALYRAKHDGRNRVVATESESASERA
                     RLIAAARRSRPLGAVLARISQRSKSVRSA"
     gene            1592..1834
                     /locus_tag="VFL49_04945"
     CDS             1592..1834
                     /locus_tag="VFL49_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911352.1"
                     /translation="MREASIHSRDGADALQLEACAERAGAALACVYSDSAEFDAALIA
                     ARRAAGVYGPRRHRRQMLVTAAGVAAGLALVAFLIV"
     gene            complement(1910..>2905)
                     /gene="glnA"
                     /locus_tag="VFL49_04950"
     CDS             complement(1910..>2905)
                     /gene="glnA"
                     /locus_tag="VFL49_04950"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7911353.1"
                     /translation="DVKYAAEPYNTGFRLDSIELPSNSATEYEGGNLGHRIGTKKGYF
                     PVPPQDSAQDMRSEMLAAMAKMGVKVEKHHHEVASAQHELGMKFSPLVIMADQMMVYK
                     YCIQQVAQIYGKTATFMPKPVYGDNGSGMHCHQSIWKAGKPVFAGNKYSDLSETCLSY
                     IAGIIKHAKAINAFTNPTTNSYKRLVPGFEAPVLLAYSARNRSASCRIPYTTNPKAKR
                     VEVRFPDPLANPYLAFAAMLMAGLDGIKNKLDPGSAIDKDLYDLPPAELKKIPTVCGS
                     LRQALGELDKDRDFLKAGGVFDDDFINSYIDLKMQEVIRFEHTPHPVEYDMYYSA"
BASE COUNT          496 a          835 c          932 g          642 t
ORIGIN      
        1 cgctaggcga tttcgtatcc gagattgtca taaaaggcgc gccaaaagcg cgtctttttg
       61 tttgccgttc gcgatcgttc aaattgtaac catttcgttt ggccggcatt catcgcgtcg
      121 atgcgcgggt ggccgggagg atgcctcagg attgattaca attcgcgaca gtctttcatc
      181 gaagcccgcc gaatagtgca aaggtttccc gacaaaacgg ggttatttta ccgtccgagt
      241 ttggcgggct gtgcgccgaa cagaggtggg aactccaatg ctgctcgcag gcggcctgca
      301 atcctcaacg gtggatttgc cgacgctgtc gttggtcgcg gtctgcattg ccggactgct
      361 cggcctgttt ctcattatcg actggatgca gcagcgtaac gtccgtgcgc tggcgtggtg
      421 gggatcggca tatctgatcg gcgcctctgc gatggcgctg ggaacgatgc cggccccgtt
      481 tatcaagctg ccggcagcgc tgcccggcgc gctgacattc ctcgcctgcg gcatggtctg
      541 gaacggggtg cggttatttc agggccgccg tgtactgcct tttgcgactt tcatcggcgc
      601 cgtgctgtgg ctcggacttg cgcaaattcc cggcatgatg gaccgcggca acggcgaggt
      661 cctcggcgca ttgatcgtgc ccgtctatac gttcttcatt gccatggagt tatggcgtga
      721 gcggcgtaga acgcgttatt cgcgggctgc cgcgatcgtc gtgccctgtc tgcacgccgg
      781 catctttttg gtgccgctgg cgatgcgcgc gttgctgcct gacgggcata gcggtgcctg
      841 gctgacggtc ttcacgctcg agacgatcct ctatgcggtt ggcaccgcct tcatcgtgat
      901 gctgatggtg aaggaccatc acgtgcacgt ctatcgcacg gcggcctcga ccgatcatct
      961 caccggcttg ctgaatcgtc gcgcattcat cgagaacgcc gcgactttat gcaagcggca
     1021 agccaggcgc ggcgagccgg tcgcggtgct gatgttcgac ctcgatcact tcaaatcgat
     1081 caatgaccgg tttggtcatt cggtcggcga cgaagtgctg cgattgttcg cgcgggtcac
     1141 gcgcgcgagc acgcgtctca acgacattat cggcaggttt ggcggcgaag agttcatagc
     1201 aatcgtcccg ggcggtatcg agattgcccg aaaaatcgcc gagcgcgtgc ggactgcgtt
     1261 cgaaaatgcc gggacagtga tcgaaagcca gccgatcggt gcgacggtca gcattggcgc
     1321 ggtctcttcg tacgatgcga caatgccggt cgaaaccctg atcgcgcggg ccgatgcggc
     1381 cctgtaccgg gcgaaacatg atggccgcaa tcgcgtcgtc gcgaccgaga gcgagagcgc
     1441 ttcggagcgg gcgcgcctga ttgcggcagc ccggcgctcg cgtccgctcg gggcggtgct
     1501 cgctcgcata tcgcagcgca gcaaatctgt ccgttcagcc tgattcagag tcgaaacgtc
     1561 tcgtttacac gccggcagta ccatttccgc catgcgggag gcatcgattc acagtcggga
     1621 cggcgctgat gcgcttcagc tcgaagcctg tgccgaacgg gcgggcgccg cgctggcgtg
     1681 cgtctactcc gattcagccg aattcgacgc cgcactgatc gccgcgcgcc gtgctgcagg
     1741 tgtgtatggc ccccggcgtc accgccgcca gatgttggta accgcggccg gtgtcgccgc
     1801 cggtcttgct cttgttgcat tcctgatcgt ttgacatcgc ccaacgaaaa cgggcgcccc
     1861 tgcggagcgc ccgtaagtcg gatggctttc gtgatgttcg ggagaatgct tacgccgagt
     1921 agtacatgtc gtattcgacc ggatgcggcg tgtgttcgaa gcggatcact tcctgcatct
     1981 tgaggtcgat gtacgagttg atgaagtcat cgtcaaaaac gccgcccgct tttaagaagt
     2041 cgcggtcctt gtccaactcg ccgagcgcct ggcgtagcga gccgcagacg gtcggaatct
     2101 tctttagctc ggcaggcggc agatcataaa gatccttgtc gatcgcggag cctggatcga
     2161 gcttgttctt gatcccgtcg agacccgcca tcagcatcgc ggcgaaggcg aggtatggat
     2221 tggcgagcgg gtcggggaag cggacctcga cgcgcttggc cttcgggttt gtagtgtacg
     2281 gaatgcggca tgacgccgag cgattgcgcg cggagtaggc gagcaacacc ggcgcctcga
     2341 agcccggcac cagtcgcttg tacgaattgg tggtcgggtt cgtgaaggcg ttgatggcct
     2401 tcgcgtgctt gatgatgccg gcaatgtagg aaaggcaggt ttccgacaga tcggaatatt
     2461 tattgcccgc gaacaccggc ttgccggctt tccagatcga ctgatggcag tgcatgcctg
     2521 agccgttgtc gccgtaaacg ggcttgggca tgaacgtcgc tgtcttgccg tagatctgcg
     2581 cgacctgctg gatgcaatat ttatagacca tcatctggtc ggccatgatc accagcggcg
     2641 agaacttcat gccgagctcg tgctgcgccg acgccacttc gtggtggtgc ttctcgacct
     2701 tgacacccat cttggccatg gccgcgagca tttcgcttcg catgtcctga gcggagtcct
     2761 gcggcggcac cgggaaataa cccttcttgg tgccgatgcg atggccgaga ttgccgcctt
     2821 catattcggt ggccgaattg gaaggcagct cgatcgaatc gagccggaag cccgtgttgt
     2881 agggctcggc ggcgtatttg acgtc
//
LOCUS       DASSRL010000240         3991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_426020, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000240 DASSRL010000000
VERSION     DASSRL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426020"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFL49_04955"
     CDS             complement(<1..1142)
                     /locus_tag="VFL49_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HET7911354.1"
                     /translation="MIPALRSFTADLYRTAVAAAHPATCLPPLLPQLPKGQLIVLAAG
                     KAAGSMAETAEAHYLDRLRIAPERLTGVAVARHGYARTLRKVHMIEAGHPIPDAAGLE
                     AADRALTLADDAGADDLVLVLMSGGASANWIAPVGGISFEDKQALTRGLLRSGANISE
                     INTVRKHLSRIKGGRLAQRAYPAKLLTIAISDVPGDDPSAIGSGPTVPDTTTLADARA
                     VIAKYKIDAPETIAQALNKSNNESPKPGDQVFSGSQFVLAARPADALHAAEAAVRAAG
                     YDCICLGDRIEGEARDVGAEHARLARELRAQGKRSVILSGGELTVTVRGKGRGGPNQE
                     YALALAIGLAGLPAIAALAADTDGTDGGSGAATDPAGALVDGESVAR"
     gene            1330..1788
                     /locus_tag="VFL49_04960"
     CDS             1330..1788
                     /locus_tag="VFL49_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7911355.1"
                     /translation="MTATAFTINGKAASVDVEPQTPLLWVVREHLKLTGTKFGCGSGL
                     CGACTVHVDGKPVRSCQIAVSKVAGKKVTTIEGLSPNGQHPLQKAWVAEQVPQCGYCQ
                     SGQIMQAAALLATTKKPTREQIVSHMDGNICRCGTYPRIVRAIQRAAREA"
     gene            1792..>3991
                     /locus_tag="VFL49_04965"
     CDS             1792..>3991
                     /locus_tag="VFL49_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7911356.1"
                     /translation="MTIHVKNGSLDTSISRRGFVAGVAGLTFSFTLGGLGRSGEAIGA
                     TQPTKFNAWVSIAADNTISIVCPAAEMGQGVYTSLPLILAEELDADWSKVKTEFAPAN
                     PKVYGNTHQLFGGAQITAASVSVPGYYMPLRMAGAQARKVLLNAVAEKWKVPVSELAT
                     DKSVITHKKSGRKISYGEVVAFANVPSEPPKVTEQELKKPSQFKLIGRTDIGRSDVPM
                     KVNGSAKYGIDVQVPGMVYASVLQSPMEGAKPKDVNAPDVMKVKGVSKVLPLPFGVAV
                     IGDTVEATREGLQALKVTWDTSSATAAGFDSEKAKAEYAAKAKDPSAETKVEYQVGDV
                     KAGLSGAAKTVEAAYWSEYTYHAQMEPMNAVAQVSEDGSSADIWTGTQFGALASLIIS
                     GILKTTPDKVRIHNQFLGGGYGRRIWPDAAIQATILSNITKKPVKLILTREEDIAAAR
                     PRPMTHHLLKAGLDNKGTIVGWHHRLVSENVDAVAAPPRFKATGGKDYIGARGLDQAF
                     YAIPNVLAEYTREDRGMRVHAWRGIGSGYNKFAAESFLDEVAAAAGKDPLALRLELTK
                     DKPRANAVLKAVAEMSDYSRKRPGRGMGIAFSDYHDTLSAGVAEISLDRGSGKIKVHN
                     FWIAVDPGLVIQPDHVRGQLESAVIYGLSAALIEQFVVKHGVVQATNFDSYPVMRMSE
                     VPEIHTRVLATNNPPTGMGEIGVVTVAPAIAGALFQLTSKRIRHVPMTAER"
BASE COUNT          809 a         1237 c         1259 g          686 t
ORIGIN      
        1 cgcgccactg attcgccatc aaccagcgcg cccgcagggt ccgtcgccgc tccgctgccg
       61 ccgtcggtgc cgtccgtgtc cgcggcgaga gccgcaatgg caggcagtcc ggccagacca
      121 atggcaagcg ccagcgcgta ttcctgattt gggccgcctc gccctttgcc ccgcactgtt
      181 acggtcagtt cgccgcccga gagaatcacc gaccgtttgc cttgcgcgcg cagttcgcgg
      241 gcgaggcggg catgctcggc accgacgtcg cgcgcttcgc cttcgatccg gtcgccaaga
      301 caaatgcaat cataacccgc agcgcgaacc gccgcttcgg ctgcgtgcag ggcgtcggcc
      361 ggccgcgcag ccagcacgaa ttggctgccg gagaaaacct ggtcgccggg cttgggcgat
      421 tcattgttcg acttgttgag cgcttgtgcg atcgtctcgg gagcatcgat tttgtatttc
      481 gcaatgaccg cacgcgcatc ggcgagggtc gtagtatccg gaacggtggg acccgagcca
      541 atcgccgaag gatcatcccc cggcacatcc gagatggcga tggtcagaag ctttgccgga
      601 taggcgcgct gcgccagacg tccgccttta atgcgcgaaa gatgtttgcg cacggtgttg
      661 atttcactga tgttggcgcc ggagcgaagc aggccgcgtg ttagcgcctg tttgtcttca
      721 aacgaaattc caccgacagg cgcgatccag ttggccgaag ccccgcccga catcaggacc
      781 agaacgaggt cgtccgcgcc cgcatcatcg gccagcgtca gggcgcgatc ggcggcctct
      841 agcccggctg cgtccggtat cggatgcccg gcctctatca tgtgtacctt gcggagtgtg
      901 cgggcgtagc catgcctggc gactgcaacg ccggtcaggc gctccggcgc aatccggagg
      961 cggtcgagat aatgcgcttc agccgtctct gccatcgacc ccgcggcttt gcccgcagca
     1021 agcactatca gttgtccctt gggcaattgc ggcagcagtg gcggcaagca tgtcgccggg
     1081 tgcgcggccg ctaccgcggt gcggtacagg tcagcggtaa aagagcgcag cgccggaatc
     1141 atagccacat tctagctgcc caccctctgg aaaacctgct tataatggat ttcagcaatg
     1201 actgaatgag cggggcaact caccggacat ttcacgcaga ttttgccgtc gtgccagcgc
     1261 agaccgccac caacgcgcgg atcaagcgcg atccaacgac aacaaaacgc ttttcctggg
     1321 agtagagcaa tgactgcaac agcattcacg atcaacggga aggcggcgag tgtcgacgtc
     1381 gagccgcaga caccgttgct ttgggttgtg cgcgaacatc tgaagctgac cggcaccaaa
     1441 ttcggctgcg gctccggcct gtgtggggcc tgcaccgttc atgtcgacgg caagccggtc
     1501 cgctcgtgcc agatcgccgt atccaaggtc gctggcaaaa aggtgacgac catcgaaggc
     1561 ttgtcgccga atgggcagca tccactgcag aaggcatggg tcgccgaaca agtgccacag
     1621 tgcggctatt gccaatccgg acagatcatg caggcggccg cattgcttgc gaccaccaaa
     1681 aaaccgacgc gcgagcagat cgtcagccac atggacggca atatctgccg ctgtggcacg
     1741 tatccccgca tcgtgcgcgc cattcaacgc gctgctcggg aggcctgagc catgaccatc
     1801 catgtaaaga acggctcgct cgacaccagc atcagccggc gcggtttcgt cgccggcgtg
     1861 gccggactga cattttcatt caccctcggc gggcttggcc gcagcggcga agcaatcgga
     1921 gcgacgcagc cgacgaagtt caacgcttgg gtgagcatcg cggccgacaa cacaatttcg
     1981 atcgtgtgtc cggccgcgga aatgggccag ggcgtctata cgtcattgcc gctgattttg
     2041 gcggaagagc tggatgcgga ctggtcgaaa gtgaagaccg aattcgcccc ggcaaatccc
     2101 aaagtctacg gcaacacgca ccagcttttc ggcggcgcgc aaattaccgc agccagtgtt
     2161 tccgtgcccg gctactacat gccgctccgg atggccggcg cgcaggcccg caaggtcctg
     2221 ctcaacgcgg ttgccgagaa atggaaagtg ccggtgtctg aactcgcgac cgacaagagc
     2281 gtcatcacgc acaagaaatc cggccgcaag atcagctacg gcgaggtggt cgcgtttgcg
     2341 aacgttccgt ccgagccgcc gaaggtgacg gagcaggagc tcaagaagcc gtcgcagttc
     2401 aagctcatcg ggcgcacgga tattggccgc agcgacgtcc ccatgaaggt gaacggctcg
     2461 gcgaaatacg gcatcgatgt gcaggtgccg gggatggtct acgcctccgt gctgcagtcg
     2521 ccgatggagg gcgccaagcc gaaggacgtc aacgcgcccg acgtcatgaa ggtgaagggc
     2581 gtaagcaagg tccttccgct gccgttcggc gtcgcagtca tcggcgacac ggtggaggcg
     2641 actcgcgaag gcttgcaggc gctcaaggta acctgggata ccagcagcgc caccgccgcc
     2701 ggcttcgatt cggaaaaagc caaagcggaa tacgcggcga aggccaagga tccgagcgcg
     2761 gaaaccaagg tcgaatatca ggtcggcgat gtgaaggccg gcctgagcgg cgcagcgaaa
     2821 acggtggagg ccgcctactg gtccgaatac acctaccacg cgcagatgga gcccatgaac
     2881 gcggtggcgc aggtgtcgga agatggctca tccgccgata tctggaccgg cacgcaattc
     2941 ggcgcgctgg cgagcctcat catttcgggc atcctcaaga ccacacccga caaggtccgc
     3001 attcacaacc agttcctcgg cggaggttac ggccggcgta tttggccgga cgccgcgatc
     3061 caggcgacga tcctgtcgaa cataaccaag aaaccggtga agctgatcct gacacgtgaa
     3121 gaggatattg ctgcggcccg accgcgtccg atgactcatc atctgctcaa agccggactg
     3181 gacaacaagg ggaccatcgt cggctggcac caccgccttg tgtcggagaa tgtcgacgcg
     3241 gtggcggcac ctccgcgctt caaggccacc ggcggcaagg actacatcgg tgcacggggt
     3301 ctcgatcagg ctttctacgc gattcccaac gtgctagcgg aatacacgcg cgaagaccgc
     3361 ggtatgcgcg tccatgcctg gcgcggcatc ggctccggct acaataagtt cgcggctgag
     3421 tcattcctgg atgaagtcgc agcggctgcc ggcaaggatc cgcttgcact gcggctggaa
     3481 ctgaccaagg acaagccgcg tgcaaacgcc gtgctcaagg ctgtcgccga aatgtcggac
     3541 tattcacgca agcggcccgg gcgcggaatg ggaatagcgt tttccgacta tcacgacacg
     3601 ctgtcggccg gagttgcgga aatctctctc gaccgcggtt ccgggaagat aaaggtgcat
     3661 aatttctgga tcgcggtcga ccctggcctg gtgatccagc ccgaccacgt gcgtggccaa
     3721 ctcgagagcg cggtcatcta tggcttgagc gccgcgctca tcgaacagtt cgtggtgaag
     3781 cacggtgtcg tccaggccac gaacttcgat agctatcccg tcatgcggat gagcgaggtg
     3841 cccgaaatcc acaccagggt tcttgcgacc aataatcctc ctacgggcat gggcgaaatc
     3901 ggcgtggtga cggttgcgcc ggcaattgca ggcgcgctgt tccagttgac gagcaagcgc
     3961 atccggcacg tgccgatgac ggcggaacgc g
//
LOCUS       DASSRL010000241         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_163691, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000241 DASSRL010000000
VERSION     DASSRL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163691"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFL49_04970"
     CDS             complement(<1..549)
                     /locus_tag="VFL49_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911357.1"
                     /translation="MQRLPEPSRAKRARLIAGGLAAAGFGLTLLVFYPGIMTYDAKYV
                     YEDIAKHTLGDWQSPAMTVLWAFVDPVAPGSASMFLLMAGAYWLAFGLLGSALAGRST
                     GLGALPPILALLPPAFVLVGVLWRDVLFAITWLLAAVMTFAVTDRPRKQRAAIQGLAL
                     AVCGLGVLLRPNSIIAAPLVALY"
     gene            complement(533..1279)
                     /locus_tag="VFL49_04975"
     CDS             complement(533..1279)
                     /locus_tag="VFL49_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET7911358.1"
                     /translation="MLGLLKTTGGILRSLRIYYGNRAHASAMDRLYGEFVDDGDLVFD
                     VGAHVGDRVASFRRLGARVVAVEPQPAMVTVLKLLHGRNPAITIEPVAVGREAGSADL
                     MINPGNPTVSTASPDFVSAAHNAPGWESQRWTKTTQVPVTTLDALIAKHGKPAFIKID
                     VEGFEAEALAGLTRPVRALSFEFTTIQREVARACIERCLTLGYSRFNSALGESQVLTH
                     EQWVDGHAMAAWLAALPHSANSGDIYAALA"
     gene            complement(1283..3061)
                     /gene="mdoH"
                     /locus_tag="VFL49_04980"
     CDS             complement(1283..3061)
                     /gene="mdoH"
                     /locus_tag="VFL49_04980"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003962.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HET7911359.1"
                     /translation="MTIGVAGHAMTWRRGLFALLFAATMIGSLGLAACALSPGGLDVA
                     DIVLLVLFAVTLPWMIAGLWNAVIGFLIMRFARDPIAAVVPEATSIRGDEPIVASTAI
                     VLCVRNEAPERIVRNLEPMLAGLSTSGFGNCFHLYVLSDTNASSIAVAEEMRIEELAA
                     QWKGRVAVTYRRRAMNTGYKAGNIRDFCERWGGDHELAVTLDADSFMTADAILRLVRL
                     MQANPRLGILQGLVVGLPSTSAFARIFQFGMRLGMRSYTIGSAWWQGDCGPYWGHNAA
                     LRLKPFIAHCALPVLSMDGTEDRHILSHDQIEAALMRAAGYEVRVLPEEDLGWEENPP
                     TLLEFIRRDLRWCQGNMQYWRFLVRPGLTAVSRYQLALAIVMFIGSPAWMAILALTAL
                     RPFFADASAGFMRGDAGIALFAWVLSMWFAPKIATAADILLRPDLRREFGGTARFVAN
                     YWIELVYSLFLVPVLWFGHTMFLIGLLFGRDIGWIGQTRDDHTVPLSLAFRSLWPHTL
                     LGAAVLIALAMTWPAAIPYAAFLAAGLAFAIPFAMVTAWPRAGAFCVRAGIGRLPEET
                     APPAALRALTLPAVETAAPTPSLSPV"
     gene            3183..>3632
                     /locus_tag="VFL49_04985"
     CDS             3183..>3632
                     /locus_tag="VFL49_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HET7911360.1"
                     /translation="MTKDGATKAAQALWYTARGKAEIRQETLQPPRPHEVRVRALFGA
                     LSRGTEALVLSGRVPRSEFERMRAPFMDGHFSFPVKYGYATVGEIEGGPAELAGRKVF
                     ALHPHQTHFNIPQDAVVVLPENVAPQRAVLAANMETALNAVWDAAPGP"
BASE COUNT          786 a         1165 c         1130 g          551 t
ORIGIN      
        1 gtagagggca accaagggtg cggcaattat cgagttcggc cgaagcagaa cgccgagacc
       61 gcagaccgcc agtgccaggc cttgaatggc agcgcgttgc tttcgcgggc ggtccgtcac
      121 ggcgaaagtc ataaccgcag cgagaagcca ggtgatcgcg aacagcacgt cgcgccacag
      181 taccccgacg aggacaaaag ccggcggaag caaagctaga atcggcggta gcgcaccaag
      241 ccctgtagac cggcccgcaa gcgccgaccc gagcagacca aaagccagcc agtaagcccc
      301 tgccatgagc aggaacatgc tggccgaacc cggcgccacc ggatcaacaa aggcccagag
      361 caccgtcatc gccggcgact gccagtcgcc caaggtgtgt ttcgcgatgt cctcataaac
      421 gtatttggca tcgtaggtca tgatgccggg ataaaagacc agcagcgtga gaccaaatcc
      481 cgccgcggcc aggccgccgg caatcaggcg ggcgcgcttt gcccgcgaag gttcaggcaa
      541 gcgctgcata gatgtcaccg gaattggccg aatgcggcag tgccgccagc caggccgcca
      601 ttgcatggcc gtcgacccac tgctcgtgcg tcagcacctg gctctctccg agcgcgctgt
      661 tgaaacggct gtagccgagc gtaaggcaac gctcgatgca ggcgcgggcg acctcacgct
      721 ggatggtcgt gaattcaaac gacagcgccc tcaccggccg cgtaaggccc gccagcgcct
      781 cggcctcgaa tccctcgacg tcgatcttga tgaaggccgg cttgccgtgt tttgcgatca
      841 gggcgtccaa cgtcgttacc ggcacctgag ttgtctttgt ccaacgctgg ctttcccaac
      901 ccggcgcatt gtgcgcggcg ctgacaaaat ccggggaagc cgtcgaaacc gtcggattgc
      961 cgggattgat catcagatct gccgacccgg cttcccggcc gacggcaacc ggctcaatgg
     1021 ttatggcggg atttcggccg tgaagcagtt tcagcacggt aaccatcgcc ggctgaggct
     1081 cgaccgcaac gacgcgcgcc cccaaccggc gaaacgacgc cacgcggtcg ccgacatgcg
     1141 cgccgacatc gaagactaga tcgccatcat cgacgaattc gccataaaga cggtccatcg
     1201 ctgacgcatg cgcgcggttg ccgtagtaga tgcggagcga ccgaaggatg ccgcccgtgg
     1261 tcttcagcag tccgagcacg tgtcagaccg ggctgaggga aggtgtcggc gccgcggtct
     1321 cgactgcagg cagcgtcagc gcgcgtaatg ctgccggtgg cgcggtttcc tcgggcagac
     1381 ggccgatgcc ggcgcggacg cagaaagccc cggcacgcgg ccaggcggtc accatcgcga
     1441 acggaatggc aaatgcgagg ccggcggcaa gaaaggccgc atagggaatc gcggccggcc
     1501 aggtcatcgc caaagcgatc agcaccgcgg ccccgagcag cgtatggggc cagaggctgc
     1561 ggaaggccag cgacaaaggc accgtgtgat cgtcccgcgt ttgcccgatc caaccgatgt
     1621 cacggccgaa cagcagtccg ataagaaaca tcgtgtggcc gaaccagaga accggcacga
     1681 ggaagagcga atagaccagc tcgatccagt agtttgcgac aaagcgtgcc gttcctccga
     1741 actcgcggcg aagatcgggc cgcaaaagaa tatcggccgc ggtggcgatc ttcggcgcaa
     1801 accacatcga cagcacccaa gcaaacagcg ctatccccgc gtcgccgcgc ataaaaccgg
     1861 cggatgcatc ggcgaaaaag ggccgcaacg ccgtcaacgc caatattgcc atccaggccg
     1921 gcgatccaat gaacatcacg atcgcaagcg ccagctgata gcggctgacg gcggtcaggc
     1981 ccgggcgcac caggaagcgc caatactgca tgttgccctg gcaccagcgc agatcgcgcc
     2041 ggatgaattc gagcagagtc ggcgggttct cctcccatcc gaggtcctct tccggcaaga
     2101 cgcggacttc atatccggcg gcgcgcatca gggctgcttc gatctggtcg tggctgagga
     2161 tatggcgatc ctctgtcccg tccatcgaca gaaccggcaa ggcgcagtgt gcgatgaatg
     2221 gcttcagcct cagagccgcg ttgtggcccc aatagggacc gcagtcgccc tgccaccatg
     2281 cgcttccaat cgtgtaggac cgcatgccga gccgcatccc gaattggaag atacgggcga
     2341 aggcgctggt cgaaggcaga cccacaacca ggccctgcag gattccaagc cgcggatttg
     2401 cctgcatcag ccgaacaaga cgcagaatcg catccgcagt catgaaactg tcggcgtcca
     2461 gcgtgaccgc gagctcgtga tccccgcccc accgctcgca aaaatcgcgg atattgccgg
     2521 ccttgtatcc ggtattcatc gcacgccggc ggtaggtcac cgcaacacgg cctttccact
     2581 gcgcggcgag ctcttcgatg cgcatctcct ctgccaccgc aatcgaggac gcattcgtat
     2641 cgctgagcac atagaggtgg aagcaattgc caaatccgga agtgctcagc ccggcaagca
     2701 tcggctcgag attacggaca atgcgttcag gcgcctcgtt gcggacgcac aggacaatgg
     2761 cggtcgaagc gacgatgggc tcgtcgccac gaattgacgt ggcctccgga acgactgccg
     2821 cgatcggatc gcgcgcgaag cgcatgatca ggaatccgat cacggcgttc cacaggcctg
     2881 caatcatcca tggcagcgtc actgcaaaca gcacaagcag aacgatatcg gccacgtcca
     2941 gtccgccggg ggaaagcgcg caggccgcaa gccccagcga cccgatcatg gtggcggcaa
     3001 aaaggagtgc gaacaggccg cggcgccagg tcatcgcgtg ccctgcgacg ccaatcgtca
     3061 tcgattgcgg aggtgagcgc tctaacacgc ggctaacgac ttctattccc cggcgggccg
     3121 tgcgaacctg atgatataac acgggaacga tctcaatagt ccaatccggg ggctcagcga
     3181 tagtgaccaa ggacggcgct accaaggctg cccaagcgct ctggtatacg gcccggggaa
     3241 aagcggaaat ccggcaggaa acgctgcagc cgccacggcc gcacgaagtg cgggtccgcg
     3301 cgcttttcgg tgcgctcagc cgaggcaccg aggctcttgt tctaagcgga cgggtccccc
     3361 gcagcgagtt tgaacgtatg cgcgcaccgt tcatggacgg tcatttttcc ttcccggtga
     3421 aatacgggta cgccactgtc ggcgaaattg aaggtggacc ggccgaattg gcaggccgca
     3481 aagtctttgc gctgcatcct catcaaaccc atttcaatat tccgcaggac gccgtcgtgg
     3541 tgctgcccga gaacgtcgcg ccgcagcgcg cggtgctggc ggcgaacatg gaaacggcgc
     3601 tcaacgcggt atgggacgcg gcacccggcc ca
//
LOCUS       DASSRL010000242         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_443787, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000242 DASSRL010000000
VERSION     DASSRL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443787"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFL49_04990"
     CDS             complement(<1..530)
                     /locus_tag="VFL49_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911361.1"
                     /translation="MVCVGLGVAASGASAADVNIRGHVGETLEGSDNYFLSNAPLGST
                     FRSLTALNLDVLARTPGWRYLLSNNVSYYNYFGDGAGQTSPKSGFPVYELFRVDHTTD
                     LARYYATASWTRADVASTQLRESGVVTGTGTIDTLRAGGGVTYDISRIDSISWTGQVS
                     HTSFSNSTQTPYNDYAT"
     gene            complement(605..1210)
                     /locus_tag="VFL49_04995"
     CDS             complement(605..1210)
                     /locus_tag="VFL49_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET7911362.1"
                     /translation="MRSVLQSGLRSGLFVCLALCAFVTIARAQEIQPGDTLQISVWQD
                     PKLDRQVVVSPDGMIAFPLAGHIKAGGITPAALENLLRARLQKNYTGKLDITVSLAAV
                     NPTEAADQRPRVYISGEVLKPGPYPAKPPIDIAQAISLAGGLSPFAASRRIQIHRKVN
                     GVDTILVFDYKAFQSGTGAPDSNLRLRSGDVIIVPERGLLE"
     gene            complement(1310..2146)
                     /locus_tag="VFL49_05000"
     CDS             complement(1310..2146)
                     /locus_tag="VFL49_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7911363.1"
                     /translation="MYEAFYGLREKPFSILPDPDLIYWGRAHRMAFSMLEFGVMNNAQ
                     FTVITGEIGSGKTTLVRHLLRRFDPRRINVGLISNTPRGRDELLQWIMMSLNLPFEGS
                     IPSLLRIFQDFLYRQRTQGRRTILIVDEAQNLGEGALEGLRMLSNINVDKMQFLQSIL
                     VGQPQLKDMLCRPELLQFAQRVSSDFHLKALAREEVGDYIGFRLSAAGAREHLFTNEA
                     CLLIADASHGIPRMINILSDMALVYGYSTDARHINRKLMQEVIEDKRNFGIFPIGAEA
                     VS"
BASE COUNT          445 a          695 c          714 g          400 t
ORIGIN      
        1 gttgcatagt cgttgtaagg cgtctgggtc gaattgctga aactcgtgtg actcacctgc
       61 ccggtccacg aaatcgaatc gatgcggctg atgtcgtaag tgacgccgcc gccggcccgc
      121 agggtgtcga tcgtcccggt accggtgacc acgccgcttt cgcgcaactg ggtggaggca
      181 acgtcggcgc gcgtccagga ggcggtggcg taatagcgcg ccagatcggt ggtgtgatcg
      241 acgcggaaca gttcgtagac cggaaagccg ctcttcggcg aggtctggcc ggcgccgtcg
      301 ccgaaataat tataatagct gacattgttg ctgagcagat agcgccagcc gggcgtgcgc
      361 gcgagcacgt cgagattgag cgcggtcagc gacctgaaag tgctgccgag cggcgcgttg
      421 gacaggaaat aattgtcgct gccctcgagc gtttccccga cgtgtcccct gatgttgacg
      481 tcagccgcag aggcgccgga cgccgcgacg cccagaccca cgcagaccat gaacgtcacc
      541 ctgcacaccg atctcttcac aacgccgggg tcctgttcgg gacgactgcc gcctggccac
      601 acacctattc aagcaagcct cgttcgggga cgatgatgac gtcgcccgac cgcaggcgaa
      661 gattgctgtc gggggcgccg gtgccactct gaaaagcctt gtagtcaaac accaaaatcg
      721 tatcgacgcc gttaaccttg cgatggatct ggatacggcg gctggccgca aaggggctca
      781 ggccgccggc gagcgagatc gcctgcgcaa tatcgatcgg cggcttcgcc ggatagggcc
      841 cgggcttgag cacctcgccg gagatataga cgcgcggcct ctggtccgcc gcctcggtcg
      901 gattgacggc ggcgagcgag accgtgatgt cgagcttgcc ggtatagttt ttctgcaggc
      961 gcgcgcgcag gagattctcg agcgccgccg gcgtaatccc tccggccttg atgtggccgg
     1021 cgagcgggaa ggcgatcata ccgtcggggc tgaccaccac ctggcggtcg agcttgggat
     1081 cctgccagac ggaaatctgc agggtatcgc cgggctggat ctcctgcgcg cgcgcaatcg
     1141 tgacgaatgc acacaaagcg aggcagacaa acagacctga ccgcaacccc gactgcaaaa
     1201 ccgaacgcat tatttccccc acgtgatttc ccccaacttg tgcgaccttc acgcgccccc
     1261 cgcccgacgt ccaggcaacg cgccgcaacg cgtcacccaa ccggcattct cagcttaccg
     1321 cttcggcacc gattggaaaa atgccgaagt ttcttttgtc ctcgatgacc tcctgcatca
     1381 gcttgcggtt gatatgccgc gcgtcggtcg aatagccgta gaccagagcc atgtcgctca
     1441 ggatgttgat catgcgcgga atgccatggc tcgcatccgc aatcagcagg caggcctcgt
     1501 tggtaaacag atgctcgcgg gcaccggccg ccgacaggcg gaagccgata tagtcgccga
     1561 cctcctcgcg cgcgagcgcc ttgaggtgaa agtcggatga gacccgctgc gcgaactgca
     1621 ggagctcggg ccggcacagc atgtccttga gctgcggctg gccgaccagg atggactgca
     1681 ggaactgcat cttgtcgaca ttgatgttgg acagcatgcg caggccttcg agcgcgccct
     1741 cgccgaggtt ctgcgcctcg tcgacgataa ggatcgtgcg gcgcccctgc gtgcgctgcc
     1801 tgtagagaaa atcctggaag atcctgagca gcgaggggat cgagccctcg aacggcagat
     1861 tgagagacat catgatccac tgcagcaact cgtcgcggcc gcgcggcgtg ttggagatca
     1921 atccgacatt gatgcggcgc ggatcgaagc ggcgcagcag atggcgcacg agcgtggtct
     1981 tgccggagcc gatctcgccg gtgatgacgg taaactgcgc attgttcatc acgccgaact
     2041 cgagcatgga gaaggccatg cggtgcgcgc ggccccaata gatcagatcg gggtcgggca
     2101 aaatcgagaa cggcttttcg cgcaatccgt aaaacgcctc gtacatcaat gctcctcaac
     2161 gcgcgcagcc ggtggcagcc gcggggccaa gcgggctgcg gcgtgttcag tgcttaccat
     2221 gcgtccgtta ctcaacaacg ttcccagata aaat
//
LOCUS       DASSRL010000243         4174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1580897, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000243 DASSRL010000000
VERSION     DASSRL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580897"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFL49_05005"
     CDS             complement(<1..992)
                     /locus_tag="VFL49_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET7911364.1"
                     /translation="MSTPIALQRRRDYAGPALFSYGFRPFFLAAGLWAVVGILLWIPQ
                     YMGKLSIPTHLSPLDWHIHEMLYGYVAATVAGFLLTAIPNWTGRLPVNGWPLAGLAAL
                     WLAGRLAMLVSAQIGGLVAAIVDTAFLATLAVVAGREIIAGENWRNLRVLTVLVVLVI
                     GNIVFHVEVLMTGTADYGTRIAIAAVILLISLIGGRIVPSFTNNWLTRNNPGRMPVPF
                     SRFDLATVVSSAIALVAWIAAPMHPISGAALIAAGCLQSVRLARWAGDRTIADRLVLV
                     LHIGYFFVPLGFVMVGLAAFVAEVPATAGIHAWTAGAIGLMTVAVMTRATLGHTG"
     gene            complement(1020..1628)
                     /locus_tag="VFL49_05010"
     CDS             complement(1020..1628)
                     /locus_tag="VFL49_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911365.1"
                     /translation="MPLQSRVSPFGELFATPARGTLMGNRGGRLHDSHRKLSARRWIN
                     RAWICCKLEFNDRHRNVWGDGYTELFFLDEVTAFAAGHRPCFECRRKDAERFALLFSG
                     KKQRAPAAAMDKILHAERLDGKAKLSDRAMLDGLPDGAMISLDGQPFALQGNRLLRWE
                     PEGYSQVRSRPRGIDVDVLTPSSIVSVLRRGYVPLWHPSATR"
     gene            complement(1691..3616)
                     /gene="ftsH"
                     /locus_tag="VFL49_05015"
     CDS             complement(1691..3616)
                     /gene="ftsH"
                     /locus_tag="VFL49_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7911366.1"
                     /translation="MNANLRNFALWVIIVLLLLALFTLFQNPGQRTTAQDISFSQLLS
                     EVDQGRVRDVVIQGPEIHGTFSNGTSFQTYAPNDPGLVQKLYSKGVAITARPPSDNVP
                     WFVSLLVSWLPFIALIGVWIFLSRQMQGAGGKALGFGKSRAKLLAEAHGRVTFEDVAG
                     VDEAKQDLQEIVEFLRDPGKFQRLGGRIPRGVLLVGPPGTGKTLIARAVAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQLLVEMDGFEANEGIILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVVGRESI
                     LKVHVRKVPLAPDVNLKTIARGTPGFSGADLMNLVNEAALMAARRNKRMVTQAEFEDA
                     KDKVMMGAERKSLVMTEEEKLLTAYHEGGHAIVAFNVKANDPVHKATIIPRGRALGMV
                     MQLPERDKLSMSREQMTSRLAIMMGGRVAEEMVFGKEKVTSGAASDIEQATKLARMMV
                     TRWGLSDELGTVAYGENQDEVFLGYQVSRQQNISEETSRKIDAEIRRLVEAGYAEATQ
                     ILTEKRADLEVLAKGLLEFETLTGDEIADLLAGKRPVRESVIEPTTPRSSAVPPAGKP
                     RPRPEPPTGEVAPQPQA"
     gene            3905..>4174
                     /locus_tag="VFL49_05020"
     CDS             3905..>4174
                     /locus_tag="VFL49_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911367.1"
                     /translation="MGLIKRGFECFELAELDRSFVTDPCHRAAEQAQCDLRGQIDKSP
                     RFYRDRRPGRQFDEQVGNGTLDGPFDGNIRSPEPPSEPGQLGGIET"
BASE COUNT          790 a         1352 c         1298 g          734 t
ORIGIN      
        1 ccggtatgtc cgagcgtcgc ccgcgtcatg accgcaaccg tcatcaaacc gatggcacct
       61 gccgtccaag cgtggatacc cgcggttgcc ggcacctccg cgacgaaagc cgccaggccc
      121 accatgacaa aaccgagcgg cacgaagaag taaccgatgt gcaacaccaa gacgagcctg
      181 tcagcaatcg tgcggtcacc ggcccagcgc gccagccgga ccgactgcag gcatccggcc
      241 gctatcagcg cagcgccgga aatcgggtgc atcggcgcag ctatccacgc gacaagcgcg
      301 atggcgctgc tgacgacagt ggcgaggtcg aagcgggaaa acggaaccgg catccgcccc
      361 ggattgtttc gggtaagcca gttgttggta aagctcggca cgatccgtcc gccgatcagc
      421 gaaatcagca gaatgaccgc ggcaatcgcg atgcgcgtgc cgtagtcggc ggtacccgtc
      481 atcagcacct caacatggaa gacgatgttg ccgatgacga gtacgacaag gaccgtcagc
      541 acccgcagat ttcgccagtt ttcgccggcg atgatctcgc gtcccgcgac cacagcaagc
      601 gtggccaaga acgccgtgtc gacgatcgcc gcgacgagtc cgccaatctg cgctgaaacc
      661 agcatggcaa gacggccggc gagccacagc gccgccagcc cggcaagcgg ccaaccgttc
      721 accggcaacc gcccggtcca gttcgggatc gccgtaagca ggaagcccgc aacggtcgcc
      781 gcaacgtagc catagagcat ttcgtgaatg tgccaatcga gcgggctcaa atgggtcggt
      841 atgctcagct tgcccatgta ttgaggaatc cagagaagaa tgccgacaac cgcccagagg
      901 ccggccgcca gaaagaacgg ccggaagccg tacgagaaca gtgcgggacc ggcatagtca
      961 cggcggcgtt gcagggcgat gggcgtcgac attttctgga cctttctttc gtgtccagct
     1021 tagcgcgttg cgctcggatg ccagagcggt acatagccgc gcctgagcac ggagacaatt
     1081 gacgacggcg tcagcacatc gacgtcgatc ccgcgcggac gcgaccggac ttgcgaatat
     1141 ccttccggct cccagcgcag caggcggttc ccctgcagcg caaatggctg cccgtccaga
     1201 gagatcatcg ccccgtcggg aaggccgtcg agcatggcgc ggtcgctgag cttggccttt
     1261 ccatcaagcc gttcggcatg aagaattttg tccatcgccg cagccggtgc gcgttgtttc
     1321 ttgccggaga acaaaagcgc aaaccgctcg gcatccttgc gccggcattc gaagcacggc
     1381 ctgtggccgg cggcgaaggc cgtcacctca tcgaggaaga acaattcggt gtagccgtcg
     1441 ccccagacgt tccgatgacg gtcgttgaat tcaagcttgc agcagatcca ggcgcggttg
     1501 atccatcgac gcgcgctcaa tttccgatgc gagtcatgca gccgcccgcc ccgatttccc
     1561 atcagcgtcc cgcgtgcggg cgttgcaaac agttccccga aaggtgagac gcgggattgg
     1621 agcggcattt ggcgcacgaa agcatgccgc ctttcgggcg gctatgctca tttatgcgaa
     1681 cagaggctgc ttacgcctgc ggctgcggcg cgacttcgcc ggtcggcggc tcgggccgcg
     1741 ggcgcggttt gcccgccggc ggcaccgcag acgagcgcgg tgtggtcggc tcgatcacgg
     1801 actcgcgcac cgggcgtttg ccggcaagca gatcggcaat ttcatcgccg gtcagcgtct
     1861 cgaactcgag caggcccttg gccagcacct cgaggtcggc gcgcttttcc gtgagaattt
     1921 gcgtcgcctc ggcatagccg gcttccacca ggcggcggat ttccgcgtcg atcttgcgcg
     1981 acgtctcttc cgagatgttc tgctggcgcg aaacctggta gccgaggaag acttcgtcct
     2041 ggttctcgcc gtaggcaacc gtgccgagct catccgacag gccccagcgc gtcaccatca
     2101 tgcgcgccag ctttgttgcc tgctcgatgt cggaggcggc gcccgaggtc accttctcct
     2161 tgccaaacac catctcctcg gcaacacggc cacccatcat aatcgccaga cgcgatgtca
     2221 tctgctcccg gctcatcgaa agcttgtcgc gttcgggcaa ctgcatgacc atgcctagcg
     2281 cgcggccgcg cggaatgatg gttgccttgt gcaccggatc gttagctttg acgttgaagg
     2341 caacgatcgc gtggccgcct tcgtgatagg cggtgaggag cttttcctct tccgtcatca
     2401 ccaacgactt gcgctcggcg cccatcatca ccttgtcctt ggcgtcttcg aattcggcct
     2461 gcgtgaccat gcgcttgttg cggcgcgcgg ccatcagggc cgcctcgttg acgagattca
     2521 tgagatcggc gccggagaag ccaggcgttc cccgcgcaat cgtcttcagg ttcacgtcgg
     2581 gcgccagcgg caccttgcgc acatggactt tcagaatgct ctcgcggccg acgacgtccg
     2641 ggttcggcac gaccacctgg cggtcgaaac ggcccgggcg cagcagcgcg ggatcgagca
     2701 cgtcgggacg gttggttgcg gcaatgagga tgatgccttc attggcctcg aagccgtcca
     2761 tttcgaccag cagctgattg agcgtctgct cacgctcgtc attgccgccg ccgaggccgg
     2821 cgccgcgatg acggccgaca gcgtcgattt cgtcgatgaa gatgatgcag ggagcattct
     2881 tcttcgcctg ctcgaacatg tcgcgcacgc gcgaagcgcc gacgccgacg aacatctcaa
     2941 cgaagtcgga gcctgaaatg gtgaagaacg gaacgttcgc ctcgccggcg accgcccgcg
     3001 cgatcagcgt cttgccggta cccggagggc cgacgagcag cacgccacgc ggaatgcgcc
     3061 caccgaggcg ctggaatttc ccggggtcgc gcaggaattc gacaatttcc tgcaggtcct
     3121 gcttcgcctc atcgacgcca gcgacgtcct cgaacgtgac gcggccgtgc gcttccgcga
     3181 gcagtttcgc ccgcgacttg ccgaagccga gcgccttgcc gccggcgccc tgcatctggc
     3241 gcgacaggaa aatccagacc ccgatcaggg cgatgaacgg cagccaggaa acgagcagcg
     3301 agacgaacca cggaacattg tcggacggcg ggcgcgcggt gatcgcaacg ccctttgaat
     3361 agagcttttg caccaggccg ggatcgttcg gtgcgtaggt ctggaagctg gtgccgttgg
     3421 agaaggtgcc gtggatctcc ggcccctgga tcacaacgtc gcggacacga ccctggtcca
     3481 cctcggacag gagttgcgag aacgagatgt cttgcgcggt ggtgcgctgg ccgggattct
     3541 gaaacagcgt gaaaagcgcc agcagcagca ggacgatgat cacccaaagc gcgaaatttc
     3601 gtagattagc gttcatcaca cgtcctgacc ccgccgcgct tgccgcacgg ccatgtgttt
     3661 tatgtatccg cttcgtggcg acttcacgtc ggactcgcca gtcaatgtag gcagcaccgc
     3721 cccccatgaa aaggccttaa gccttaatat tctagcgtct tttggcattg cccgcccggg
     3781 gaggtcggcg tttggttaag agcttgcgac ggcgcggcgg tgcccgttcg acgataattt
     3841 gaggaccctt aagcgtcacg attgcgcctg cgagagtgcg ccgaaaacga tcttctgcgc
     3901 cgttttgggc ctcatcaagc gcggttttga gtgtttcgag cttgccgagc tcgaccggtc
     3961 cttcgtcacc gacccttgcc atcgcgcggc cgagcaggcg caatgcgacc tccgcgggca
     4021 aatcgacaaa tcgccgcgct tctatcgcga tcgccggccc ggcaggcaat tcgacgaaca
     4081 ggtcggcaac ggcacgctcg acggcccgtt cgacggcaac atccgctcgc ctgagccgcc
     4141 gagcgagcct ggccaactgg gcggcatcga gacc
//
LOCUS       DASSRL010000244         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1546203, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000244 DASSRL010000000
VERSION     DASSRL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546203"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFL49_05025"
     CDS             <1..311
                     /locus_tag="VFL49_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7911368.1"
                     /translation="FGDWVSYWIGLKLEYTVAHIWPLSKRPDLIPRGEVFMKKWGIPG
                     IFIGRFFGPLRAIVPLIAGIFEMPFWHFQVANFTSAFVWGWTLLTIGDVISKAVIWLW
                     "
     gene            complement(365..901)
                     /locus_tag="VFL49_05030"
     CDS             complement(365..901)
                     /locus_tag="VFL49_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7911369.1"
                     /translation="MRRLTMLAGALGAALLMSSGAFAQVPADPNNPNENVPDKYNFTP
                     YGETIKLADAKKVAEAALAEATKRGWPMCIAVVGPSGDLVYFEKMDDCQYASIEISQN
                     KAKAAAVYRRPTVVFERLIAKGQYFAYLPTLGAGFIGSRGGNPLVAGGKIVGAIGVSG
                     GTGSQDDTISQAGVAALK"
     gene            1160..2539
                     /locus_tag="VFL49_05035"
     CDS             1160..2539
                     /locus_tag="VFL49_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7911370.1"
                     /translation="MAETAFEQALAARTAAMLDACTRCGKCVEICPMAQPAGIGNAAP
                     ESVIAGVLDIVRTGEGPEASKAWAKGCALTGDCIAACDEAVNPRFLLAMARVALARQS
                     SELRSRRKQGVENFRLVADGVNVLARMQLSENDLARLGQYVNERLENGSTAKPGERPD
                     FVFYTGCNVLKTPHMALTALDIMDALGVTYRVMGGPTHCCGVIQLRAGDTDVSGRVAT
                     SSLDKLAEGKAGVISWCASCHVQFTETTIPTIEKVRGSRPFEMTPFMLFLKTRFEDLR
                     PMLKHPVPMRIALHKHPGVKGVVEAGTELLRMVPGIEIVDLHQPAVGLMSNSLNALPE
                     YKRGLQLAELEAAAAAGVDALVAIYHVDHRELCAHERDWPFRVINILDIVGASMGLHH
                     DDHFKRLKIMQDAEAIVADCRDMIAAYGIEPAFAREVVIKAMLKEQPLPLRGRAVDGS
                     GPAAYIARP"
     gene            2713..>3527
                     /gene="argC"
                     /locus_tag="VFL49_05040"
     CDS             2713..>3527
                     /gene="argC"
                     /locus_tag="VFL49_05040"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7911371.1"
                     /translation="MATKAKIGVLGASGYTGGELVRVLLRHPRAEIVLLTAERSAGRA
                     MRDVFPQFSPFTLPQLVAIDGVDWKNAALDVAFCALPHATTQKVIKDLMAKSPATKVV
                     DLSADFRLSDTAAYAKWYGHDHHAPELQKEAVYGLTEIHRREIRQARLVANPGCYTTC
                     AQLPLLPLIKNKAIELDEIVVDAKSGMTGAGRAAKESMLFSEVSEGFHAYGVGHHRHM
                     AELDQEFSLAAGQEVIVTFTPHLVPMNRGILSTIYVRGRRGRTAEDLHAILLK"
BASE COUNT          673 a         1060 c         1101 g          693 t
ORIGIN      
        1 cgttcggcga ctgggtgtcg tattggatcg ggctcaagct tgaatacacg gtcgcgcata
       61 tctggccttt gtcgaaacgg cccgatctca ttccccgcgg tgaagtcttc atgaagaagt
      121 ggggtattcc cggaatattc atcggccggt ttttcgggcc gctccgtgcc atcgtgccgt
      181 tgatcgcagg catattcgaa atgccgttct ggcattttca ggttgcaaat ttcacatccg
      241 cttttgtgtg ggggtggaca ttgctgacaa tcggcgacgt gatttcgaag gccgtgatct
      301 ggctgtggtg actcgttgtt gaaacggcgt ggccggcaga ggcccgccac gccatttttc
      361 aaacttactt caacgccgca acgccggcct gggaaatggt gtcgtcctgt gagcccgtgc
      421 cgccgcttac gccaatcgct ccaacgatct tgccgcccgc tacgagcgga ttgccgccac
      481 gcgaaccgat aaatcctgca ccgagcgtcg ggagataagc gaagtactgg cctttggcga
      541 tcaggcgctc gaacaccacg gtcggacgac ggtaaaccgc tgccgccttt gccttgtttt
      601 gggaaatttc gatggatgca tactggcagt catccatctt ttcgaaatag acgaggtcgc
      661 cagagggccc gacaacggca atgcacatcg gccagccgcg cttggttgct tcggccagcg
      721 cggcctcggc gactttcttg gcatcggcaa gctttatggt ttcgccgtaa ggcgtgaaat
      781 tatatttgtc cggcacgttc tcattcggat tgttcgggtc ggccggcact tgcgcaaaag
      841 cacccgagct catcagcagc gcggcgccga gagcgcccgc cagcatagtc agacgacgca
      901 ttatgtacct cccacgtgaa agccggttgg gaccggcatt gcttgcctgg ccagggcggc
      961 gcaggtttaa acgcaacgag tttagccaac cgcacaggcg ccgcctatat gggtcccggc
     1021 tctcagggcc gcgcgcggaa taacgcaccc ggcacggggt tagccgcggc tacatcgaga
     1081 aaaccggcaa cggctatcgg ccttcttgtt tgcgacgggc cgcgctaagt tgctgctttg
     1141 cggcagcggg aaatgttcca tggccgagac ggcattcgag caggctttgg cggcgcgcac
     1201 ggcagcaatg cttgatgcct gcacgcgctg cggcaaatgc gtggagattt gtccgatggc
     1261 gcagccggcc ggtatcggca acgctgcgcc ggaaagcgtg attgcgggcg tgctcgatat
     1321 tgtccggacc ggcgagggac cggaagcatc aaaggcatgg gccaaaggct gcgcgctgac
     1381 aggcgactgc atcgcggcct gcgacgaagc tgtcaatccg cgcttcctgc tggcgatggc
     1441 gcgcgtcgcg ctcgccaggc aatcctctga actgcgcagc cggcgcaagc aaggcgttga
     1501 aaatttccgg ctggttgccg acggcgtgaa tgttctcgcc cgcatgcagc tgagcgagaa
     1561 cgatcttgcc cgcctcggcc agtatgtgaa cgagcgtctc gagaatggct cgaccgcgaa
     1621 accgggcgag cggccggact ttgtcttcta caccggctgc aacgtgctca agacgccgca
     1681 tatggcattg acggcgctcg acatcatgga cgcgcttggc gtgacttatc gtgtcatggg
     1741 cggcccaacg cattgctgcg gtgtcattca actgcgcgcg ggcgatactg acgtctcggg
     1801 ccgcgttgcc acgagttcgc tggacaaact cgcggaaggg aaagcaggcg tcatatcctg
     1861 gtgcgcgagc tgtcacgtcc agttcacgga gacgacgatc ccgaccatcg agaaggtgag
     1921 aggatcacgt ccgttcgaaa tgaccccgtt catgctgttc ctgaagaccc ggttcgaaga
     1981 tcttcggccg atgctgaaac atccggttcc gatgcgcatc gcgctgcaca agcatcctgg
     2041 cgtcaaaggc gtggtcgaag ccggcaccga gcttctgcgc atggtgcccg gcatcgagat
     2101 cgtcgacctg caccagccgg ccgtcggcct gatgagcaat tcgttgaatg cgctgccgga
     2161 gtacaaacgc ggtcttcagc ttgccgagct tgaggctgcg gctgccgcag gagtcgacgc
     2221 gctggttgcg atctaccatg tcgatcaccg cgagctctgc gcgcacgagc gcgactggcc
     2281 cttccgcgtc atcaatatcc tcgacattgt cggcgccagc atgggcctgc accacgacga
     2341 tcacttcaag cggctgaaga tcatgcagga tgccgaggcg atcgttgccg actgcaggga
     2401 catgatcgcg gcctacggca tcgagcccgc tttcgcgcga gaggtcgtca tcaaggccat
     2461 gctgaaagag cagcctttgc cgctgcgcgg gcgcgcagtt gacggatcgg ggcctgcggc
     2521 ttatatcgcc cgcccatgac gatgcttttg gcaaagaaag agccgcgccg ccgccgtgcc
     2581 actcaggcac gggcgtaagg cgatcgtcac ccgtgccgcc ggacgtgagg ccgcggcacg
     2641 tttctccaag acaaaccgcg ctgttggcct tcttccggta gttctttaag acttcggaac
     2701 aaggatcgat tcatggccac aaaagcgaag atcggggtgc tgggagcctc cggctacacg
     2761 gggggcgagt tggtgcgcgt gctgttgcgc cacccgcggg cggaaatcgt gctgctgacg
     2821 gccgaacgca gcgcgggcag ggcgatgcgg gatgtgtttc cgcagttttc gccctttacg
     2881 ctgccgcaac tcgtcgccat cgacggcgtc gactggaaga acgcggcact ggacgttgcc
     2941 ttttgcgcct tgccgcacgc gacgacgcaa aaagtcatca aggatctgat ggcgaaatcg
     3001 cccgcgacca aggttgtcga tctgtcggca gactttcggc tgtccgatac cgcagcctat
     3061 gcaaaatggt atggccatga ccatcacgcg cccgaactgc agaaagaggc cgtctacgga
     3121 ttgaccgaaa ttcatcgccg cgagatcagg caggcacgcc tggtcgccaa tccgggctgc
     3181 tacacgacct gcgcgcagct gccgcttctc ccgctcatca agaacaaggc gatcgagctc
     3241 gacgagattg tcgtcgatgc aaaatccggc atgaccgggg ccgggcgggc cgcaaaagag
     3301 tccatgctct tttccgaagt ctcggaaggc ttccacgcct acggcgttgg tcatcaccgg
     3361 catatggcag agctcgacca ggagttctcg cttgccgccg ggcaggaggt gatcgtcaca
     3421 ttcacgccgc acctggtgcc gatgaaccgc ggcatcctgt cgacgatcta cgtccgggga
     3481 cggcggggcc gaaccgccga agatcttcat gcgatacttt taaagtt
//
LOCUS       DASSRL010000245         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_846276, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000245 DASSRL010000000
VERSION     DASSRL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846276"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFL49_05045"
     CDS             <1..516
                     /locus_tag="VFL49_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7911372.1"
                     /translation="VHEDELLGTGGTILVNRKWFGAGPFLVAHADNLTNFNVAEFIAA
                     HKQRPHPCVLTMLVFWTDSPTSCGILETGPRGIVTGFHEKAENPPGDLANAAVYICEP
                     EIAAFIASLGKPNVDFSTEVIPAFLNRIYAVRTNGYHRDIGTIEALSRAQAECKTMPP
                     GTLSQEARRRG"
     gene            525..1460
                     /locus_tag="VFL49_05050"
     CDS             525..1460
                     /locus_tag="VFL49_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911373.1"
                     /translation="MKVFVTGGCGYKGTVLVPKLLERGHQVVAFDIQWFGNRLRPHPS
                     LTVIKGDVRNPEEYDLSGIDAVIHLSSIANDPAGDLDPKLTWEVNCLATMQFADKARR
                     QGVKRFVFASSGSVYGVKKELQVTEDLELLPISEYNKTKMCAERILLSYKDEMAVQIV
                     RPATVCGFSPTMRNDVSVNMLTMLALKNRRITVFGGDQVRPNVHMDDITDLYIFLLDH
                     PEHTGIFNAGFENVSIMQIAQMAARHVDAEIVVTPSNDPRSYRVNSDRLLAAGFRPKK
                     TIEDAIKELVGLYRQGVWTDDPSFHRMSWMQKEVV"
     gene            1460..2080
                     /locus_tag="VFL49_05055"
     CDS             1460..2080
                     /locus_tag="VFL49_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7911374.1"
                     /translation="MLDPSAARKSMLPKVRDGHFEDYSRRLSAALEACDWHPVSDLAD
                     ELLDCARTGRQVFIAGNGGSAANAVHLANDFLYAWSKQQGRGIRVHALPANTAVMSCL
                     ANDEGYDKVFSGQLAVHAEKGDLLLVFSSSGNSPNILRALEQARIIGMKSFAVLGFDG
                     GKAKSMCDVPIHFAVDDTQIAEDVQLILGHMIMQHLNAHRDDLGRP"
     gene            2084..>2856
                     /locus_tag="VFL49_05060"
     CDS             2084..>2856
                     /locus_tag="VFL49_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7911375.1"
                     /translation="MADPKFLILGSNSFSGATFVDYLTEQGHDAFATSRSNEPHDALL
                     PYKWHKRPGRVRFQRIDINHDLGALGVLLKHERFTHVVNFAAQSMVGQSWDRPDDWMM
                     TNVVSTVRLHVLLRDIDFLDRYVHVTTPEVYGSTDGWIRENTPFNPSTPYAVSRAAGD
                     MSLKTFVDTYNLPAVSTRAANVYGPGQQLYRIIPRTVLAAMTGQKLTLDGGGVSKRVF
                     IHMRDVADATLKIALSGRLGETYHISGHDLISIRELVEI"
BASE COUNT          620 a          780 c          825 g          631 t
ORIGIN      
        1 gttcatgaag acgagctctt gggcacgggg ggcacaattc tcgtgaatcg caaatggttc
       61 ggcgccggcc cctttcttgt cgcgcatgcc gacaatttga caaatttcaa tgtcgcagaa
      121 tttattgccg ctcacaagca acgaccacac ccgtgtgtcc ttacgatgct ggttttttgg
      181 accgactcac cgacatcatg tggaattctc gaaaccggac cccgaggcat cgtcacgggc
      241 ttccacgaga aagctgaaaa cccgcccggc gatcttgcca atgcagcggt ctacatttgc
      301 gagccggaaa tcgccgcgtt catagcctcg ctcgggaagc cgaatgtgga cttttcaact
      361 gaagtgattc ccgcattttt gaatcgtatt tatgcggtaa gaacgaatgg atatcatagg
      421 gatataggta caattgaggc tttgtctcgc gcacaggcgg agtgtaagac gatgccgcca
      481 ggaacgttga gccaggaagc ccgtcgtcgg ggatgaggcg ttgtatgaaa gtattcgtga
      541 caggggggtg cggctataag gggaccgttc tggtgccgaa gctcctggag cgcgggcacc
      601 aggtggtcgc cttcgacatc cagtggttcg ggaacaggtt gcggccgcat ccgagtctga
      661 ccgtcatcaa aggggacgtg cgcaatccgg aggaatacga cctttccggc atcgacgcgg
      721 tcattcatct ttcttcgatc gcaaatgacc cggccggaga tctcgatccg aagctcacct
      781 gggaagtgaa ttgcctggcg accatgcagt tcgccgacaa ggccaggcgc cagggagtca
      841 agagattcgt cttcgcctcg tccggaagcg tctacggcgt caaaaaggag ctccaggtca
      901 ccgaggatct cgagctgctg ccgatcagcg agtacaacaa aaccaaaatg tgtgccgagc
      961 gtatccttct ctcatataaa gacgagatgg cggtgcaaat cgtgcggcct gcaacggtgt
     1021 gcggattttc gccgacaatg cgaaacgacg tgtccgttaa catgctgact atgctggctc
     1081 tcaagaatcg ccgcatcact gtttttggcg gagaccaggt acggccgaac gtccatatgg
     1141 acgacatcac cgatctttac attttcctgc tggaccatcc cgagcacacc ggcatattca
     1201 atgccggctt tgaaaatgtt tcaattatgc agatagccca gatggctgcc cgtcacgtcg
     1261 acgccgagat cgtcgttacg ccgtcgaatg atccgcgttc atatcgggtc aattccgatc
     1321 gccttctggc cgccggcttc aggccgaaga agaccatcga ggatgcgatc aaggagctcg
     1381 ttggactata ccgtcagggg gtttggaccg acgacccgag cttccatcgt atgtcgtgga
     1441 tgcagaagga ggtcgtataa tgcttgatcc gtctgctgcg aggaagtcga tgctgcccaa
     1501 ggtcagggac ggtcacttcg aggattatag tcgacgcctt tcggcggcgc tcgaggcctg
     1561 cgactggcat ccggtttccg atctggcgga cgagctcctt gattgcgcgc gcaccggccg
     1621 gcaggtcttt atcgccggaa atggtggaag cgctgccaac gccgtccacc tcgccaacga
     1681 ttttctatat gcctggtcga agcagcaagg tcgcggcatt cgcgtgcatg cgctgccggc
     1741 caataccgcc gtgatgagct gccttgccaa cgatgagggc tatgacaaag tcttttccgg
     1801 ccagctggcg gtccacgctg agaaaggcga tctgttgctt gttttctcaa gcagcggcaa
     1861 ctcacccaat attttgcggg cgctcgagca ggcaagaatt attggcatga agagctttgc
     1921 cgtgctgggg ttcgacggcg gcaaggcaaa gtcaatgtgt gatgttccca tccacttcgc
     1981 agtcgatgac acgcagattg ccgaagacgt ccagctgatc ctgggacata tgatcatgca
     2041 gcatcttaat gcacaccggg acgacctggg acgaccgtga cctatggccg atccgaaatt
     2101 tctgatcctg gggtcgaact cgttttcggg cgctactttc gtcgactatc tgactgaaca
     2161 aggccacgac gcctttgcga cctcgcgctc gaatgagccg catgatgcgc tgttgcctta
     2221 taaatggcac aagagaccag gtcgcgtgag gttccagcgc atcgacatca accacgacct
     2281 cggtgctctc ggcgtgctgc taaagcacga acggttcact cacgtggtca attttgctgc
     2341 ccagtcgatg gttggacaaa gttgggatcg cccggacgac tggatgatga caaatgtcgt
     2401 gtcgaccgtg aggctgcacg tgctgttgcg cgacatcgat ttcctggatc gatacgtgca
     2461 tgtgacaacg cccgaagtct acgggtcgac cgacggctgg attcgcgaaa acactccgtt
     2521 caatccgtcg acgccttatg ccgtttcgcg tgcggcggga gacatgagcc tcaagacctt
     2581 cgtcgacacc tacaatctgc ctgcggtttc gacccgggcg gccaatgtgt acggaccggg
     2641 acagcagctt taccggatca ttccacgtac ggtacttgcg gcaatgaccg gccaaaagct
     2701 cacgcttgat gggggcgggg tgtcgaagcg cgtctttatc catatgcgcg atgttgcgga
     2761 cgcaactctg aaaattgcat tgagcggccg gttgggcgaa acgtatcata tatcgggcca
     2821 tgatctgatt tcgatccgcg aactcgtgga aatcat
//
LOCUS       DASSRL010000246         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_23754, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000246 DASSRL010000000
VERSION     DASSRL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23754"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFL49_05065"
     CDS             complement(<1..345)
                     /locus_tag="VFL49_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7911376.1"
                     /translation="MDRVTSMTSFVKVADNGGFSAAARRLNLSTGVVTGHIRSLEDHL
                     GVRLFNRNTRKVSLTDVGQAYYERCVQILAEIDDADQIAEALQSKPRGFLHLNLAPVL
                     PMVFAPIIAEYTA"
     gene            543..1826
                     /locus_tag="VFL49_05070"
     CDS             543..1826
                     /locus_tag="VFL49_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET7911377.1"
                     /translation="MRDDALIAEKVRTAGDDNRNGETVDCKYQSAHVRIPHAPAANAA
                     PAGKVPAKAGAKRGRFAVPAAILLAGVTAGGWYGWHWYTVGRFLVETDDAYIQADNVT
                     VSPQVAGYISELLVTDNQPVKKGEVIARINDRVYRAAVAQARAALDIQIANAHNVDAQ
                     IARQGSIIEQMKADVESAQAQLEFARQEQSRYAKLMKDGAGTVQKAQETDSALRAKKA
                     VLDKSRANLDASQHQLAVLHTRMEQAQAGIEHAQAALEQAEINLGYTTITAPRDGVVG
                     DRTVRVGQLVQPGTRLLTLVPMHDVYVVANYKETQLADMKRGQPVDIRVDGYPDAVLH
                     GHVDSLSPGSGAQFALLPPENATGNFTKIVQRVPVKIVLDRDAPLSGTLRPGLSVVSS
                     VDIRPVHKILAIEHKAPHGQYEAALPVGRRPGEEN"
     gene            1830..>2389
                     /locus_tag="VFL49_05075"
     CDS             1830..>2389
                     /locus_tag="VFL49_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7911378.1"
                     /translation="MTGAAQTSMPAAKVMARDWIAIWGALLGAFMAVLDIQITNASLK
                     DIQGGIAASLDEGTWISTSYLVAEIIVIPLTGYLGGVFGVRRYLMVNAALFICFSMVC
                     GTAGNLDEMVLFRACQGFTGGVMIPMAFTILNLKLPPQKRAMGMAMFGLTATLAPAIG
                     PTIGGLITDRYGWPFIFYINLLPGLLL"
BASE COUNT          456 a          762 c          742 g          429 t
ORIGIN      
        1 ggccgtgtat tccgcgatga tcggagcaaa gaccatcggc aggaccgggg ccagattgag
       61 gtgcagaaag ccgcgcggct tcgactgcag cgcctcggcg atctggtcgg cgtcgtcgat
      121 ttccgcgagg atctggacgc agcgctcgta ataggcctgt ccgacgtcgg tgaggctgac
      181 cttgcgcgtg ttccggttga acagtcgcac accgaggtga tcttccagag acctgatgtg
      241 cccggtgacc acaccggttg aaagattgag ccgccgcgcc gctgcggaaa acccgccatt
      301 gtcggccact ttcacaaacg acgtcatgct tgttacgcga tccatgcctg cgatccatcg
      361 tttgacgccg cgcgtgcggt ggcgacgcca tttatgcggg ccggtcgaaa ggtgttctct
      421 gaagtttcca aattatcaat cggccgcctg agcgccattc ttccgaccat gaggtccgca
      481 gcctgcgccg gcacttttcg accggatcgt ggccaacgca tggcagttgc cgggagaatg
      541 acatgagaga cgacgcattg atcgccgaaa aagttcgaac cgcaggcgac gacaatcgga
      601 acggcgaaac agtcgattgc aaatatcaat ctgcgcacgt ccgcatcccc catgcgccgg
      661 cggccaacgc tgctccggca ggaaaggttc cggccaaggc cggcgccaaa cgcgggcgtt
      721 tcgctgttcc tgccgcaatt cttctggcgg gcgtgacggc gggcggatgg tacgggtggc
      781 actggtacac ggtcggccgt tttctcgtcg aaaccgacga tgcctacatt caggcggaca
      841 acgtcacggt cagtccgcaa gtcgccggtt acatcagcga actgctcgtc accgacaacc
      901 agccggtcaa gaagggtgaa gtcatcgccc gcatcaacga tcgcgtttat cgtgccgccg
      961 tggcccaggc gcgcgccgcc ctcgatatcc agatcgccaa tgcacacaat gtcgatgcgc
     1021 agatcgcgcg ccaaggctcg atcatcgagc agatgaaggc cgatgttgag tcggcacagg
     1081 cacagctgga attcgcccgg caggagcagt cccgctacgc aaaactgatg aaggacggcg
     1141 caggcactgt ccagaaagcg caagaaaccg attccgccct gcgggcaaaa aaggctgtgc
     1201 tcgacaagtc ccgcgccaat ctcgacgcct cgcaacatca gctcgcggtc ttgcataccc
     1261 ggatggagca ggcgcaggcc ggcatcgagc acgcacaggc cgcactcgag caggcagaga
     1321 tcaatctcgg atacacgacg atcacggccc cgcgggacgg cgtcgtcggt gaccgtaccg
     1381 tgcgggtcgg acagctggtg cagccgggaa cgcggttgct gacgctggtg ccgatgcacg
     1441 acgtatacgt ggtcgcgaac tacaaggaga cgcagcttgc cgatatgaag cgcgggcagc
     1501 cggtcgacat ccgggtcgac ggttatcccg atgcagtgtt gcatggccac gtcgacagtc
     1561 tctcaccggg atcgggcgca cagttcgcgc tgctgccgcc ggaaaacgcc accggcaact
     1621 tcaccaagat cgtacagcgc gttccggtca agatcgtgct cgaccgggac gccccgcttt
     1681 ccggcacgct gcggcccggc ctgtcggtgg tctcctcggt cgatatccgc cccgtgcaca
     1741 agatccttgc gatcgaacac aaggcgccgc acggccaata cgaagccgcg ctgcccgtcg
     1801 gccggcgtcc gggcgaggag aactaggtca tgaccggcgc ggcacaaaca tcgatgccgg
     1861 ccgccaaggt gatggcgcgc gactggatcg cgatctgggg cgcgctgctc ggtgccttca
     1921 tggcggtact cgatatccag atcaccaacg cgtcgctgaa ggacatccag ggcggcatcg
     1981 cggcttcgct cgatgagggc acctggatct ccacctctta cctcgtcgcc gagatcatcg
     2041 ttattccgct caccggttac ctcggcggcg tgttcggcgt gcgccggtat ctgatggtca
     2101 atgccgcgct gttcatctgt ttctcgatgg tctgcggcac ggccggcaat ctcgacgaga
     2161 tggtgctgtt ccgggcctgc caaggcttca ccggcggcgt catgatcccg atggccttca
     2221 ccatcctgaa tctcaagctg ccgccgcaga agcgcgcgat gggcatggcg atgttcgggc
     2281 tgactgcaac gcttgcgccg gccatcgggc cgacgattgg cgggctgatc accgaccgct
     2341 acggctggcc ctttattttc tacatcaacc tgcttcccgg cctgctgct
//
LOCUS       DASSRL010000247         5035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_548975, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000247 DASSRL010000000
VERSION     DASSRL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548975"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5035
                     /locus_tag="VFL49_05080"
     CDS             <1..>5035
                     /locus_tag="VFL49_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7911379.1"
                     /translation="SVGYVECHATGTIVGDPLEIEALNMAFRKQTKRNQYCAVGSVKA
                     NIGHPEQAAGIAGLIKTALVLHHKQIPPSINYEVPNPAIEFASSPFYVNTGLRNFPRA
                     DTPRRAALNSLGIGGTNAFAVLEEAPTAAATTSQSQVNLPCLVTLSAKSADALVAHVE
                     RLLKWLDDNPEAPVGDICYTTNISRSQFAFRFAAPARSLAQLKNSLATWLRTASQAGS
                     GLQHTGNAPIAFMFSGQGAQQAGMARQLYRTHSVFRNAMDRCDVLARPHLERSLLEVI
                     FAADGEDALVSRTDYTQPALFAVEYALAELVKSWGISPDAVIGHSLGEFVAACAAGVM
                     TLEDAIRLVVARGALMHKMPGGGGMAAIFASESAVRALIDKVAPEIAVAAMNGPLNTV
                     ASGDRSALRMLLGELDRQHISYRELHVSNAFHSPRTESILDDLQNVASQITYYPPKVP
                     LISNLTGALMVAAPDEIYWRRHLREPVRFGDGMLALAELGCRSFLEIGPHPVLLPIAQ
                     ACLGARGRSTSWVATLNRQKPDVDSITEMLAALYLAGHNINWAAVHADSSWSRIPLPT
                     YPFQRKRYWLEDNAVHTERARKVVERIHPLVGTRTNFASNGPCYEARYGTHHAGFLAD
                     HKVAGTVVLPTTAELEAATAVGRMHFGTSRISFDNAMHHQAMSFANGDDRTVRVLVTP
                     LNSDKASFKLVSKDAEDPRVWHTHMTGTLRKSEMTSRPAFSANQIRDRCRQTLSAADF
                     YDRLDKLGLEYGPGFRGVRELYLGQHETLTKVSLPDGLANTQYLMHPAFLDACLHAYP
                     LVLDGVGKAGSDGRQSYLPISLEGYRCYQDGIDKAWVHTRLRSVGKDDTQVVDIQVYD
                     DTERPVADLEGLSVRLLPLDKVQQPQVNADDLFYRSVWRKSVRRTGKTGEDGVRKSWI
                     IFADARGVAAALADKMEAAGHHCHVVSRGGSFARQRARAWTVNERRPQDFRKLLAEVA
                     ASEALPCGGLVYLWGLDAPSIDGLTLARLKSGSEMMCRGALAILHALSETRSANPDGR
                     RLWFVTANTQNTQVGGQCVDPVQACLWGLGRTAAIEYPGTWGGLIDLQLNGAPTQDID
                     ALAEELLNPDGETQIVISAGKQRHVPRFVRQSLSELALQSPPVRGDATYLVTGGLGML
                     GRSVAKWLIGKGAKQLVLTGRSASRDAIKKLFSAEEIDAASIEVAACDISRRDDVRRL
                     MRTIGKKFPPVKGVVHSVGVLDDGILAQLDWDRFSRVFESKVYGSWLLHECTKSLELD
                     FFILQSSVLSVLGSAGQANYSASNTFVDSLAAHRRVAGLPAKAINWSAWSGGGLATAS
                     GARGEAMWSSLGVKFVSPDLAMESFDRLMHRDVDQIAVVIADWPTYAGKVGNPPFLAE
                     LLNGREAPGPSGSARINGEPAAAVAQMDGGTRAELLNRLQQRIMTELGFVDPIDPDRP
                     LNEVGLDSLRSVTLANNLEDEFGVLISISQLISGPTINQLVDHLSGLFAGTEKNGAAL
                     AAPVPTLRNGKDRSLNTLAVQLINDRASDEAVADPAEDRSGPEIYRETAANEKISYGG
                     KLTNGSGAQNFAATFLRPAGEAVSRGRGKWLIAPRPNPDARARIFCFPYAGGGLVSFR
                     AWAQLFDDAVEVVAVEAPGRGTRIDETAIDDLDTFVEGLLPEMVGWLDRPSAFFGHCL
                     GGLTMFATLQ"
BASE COUNT         1018 a         1429 c         1574 g         1014 t
ORIGIN      
        1 cagcgtcggg tacgtcgaat gccacgcgac cggcacaata gtgggcgatc cgctggagat
       61 cgaagcgctg aacatggcgt ttcggaagca gaccaaacga aaccagtatt gtgctgttgg
      121 ttcggtgaag gccaatatcg ggcatccgga gcaagcggcg ggaattgccg gtctgatcaa
      181 aaccgcactc gtgcttcatc acaaacaaat tccgccgagc atcaactatg aagtacccaa
      241 ccccgcaatc gagttcgcgt cgtcgccatt ctacgtaaac accggactcc ggaattttcc
      301 ccgtgcggat acgccgcggc gagccgcatt aaacagcctg ggtatcggcg gaacgaatgc
      361 tttcgcggtg ctcgaagaag cgccgacggc ggctgcaacc acaagccaat cacaggtgaa
      421 tttgccgtgc ctggttacgc tatccgccaa aagcgccgac gcgctggtgg cgcatgttga
      481 gcggcttctg aagtggttgg acgataatcc cgaggcgccg gtcggtgaca tttgttacac
      541 caccaatatc agcagaagcc agttcgcctt tcgcttcgcc gcgcccgcac ggtcgctcgc
      601 gcaactgaaa aatagcctcg cgacatggct gcgaaccgca tcccaagcag gttcgggctt
      661 acagcacacc ggcaatgcgc caatcgcttt catgttttcc ggccagggcg cacagcaggc
      721 cggcatggct cggcaattgt atcgcacgca ttctgttttt cggaatgcca tggatcgctg
      781 tgatgtcctg gcccgaccgc atcttgagcg cagtttgctc gaggtgattt ttgccgccga
      841 tggtgaggac gccctggtca gccgcacaga ttacacgcag cccgcgctgt ttgcggtcga
      901 atatgccctg gccgagcttg tcaaatcctg gggcatttca cctgacgcgg tgatcgggca
      961 cagcctgggt gagttcgtcg ccgcttgtgc ggcgggcgtc atgacgcttg aagacgcaat
     1021 acgactcgtc gttgcgcgcg gcgcgttgat gcacaagatg ccgggcggcg gaggcatggc
     1081 cgcgatattc gcttcggaat ccgccgtacg cgcgctgatc gacaaggtcg ctcctgaaat
     1141 cgccgtggcc gcaatgaacg gacctctgaa tacggtcgca tccggtgatc ggagtgcgtt
     1201 gaggatgctg ttgggcgaac tggaccggca acatatcagc tatcgtgaat tgcatgtgtc
     1261 caacgccttt cactctcccc gtacggagtc gatcctcgac gatctgcaga acgtagcgag
     1321 ccagatcaca tattacccgc cgaaagtacc cctgatctcc aaccttaccg gcgcgttgat
     1381 ggtggctgca cccgacgaaa tttattggcg tcggcatctg cgggaaccgg tgcgtttcgg
     1441 cgacggaatg cttgcgcttg ccgaactcgg ttgccggtct tttctggaaa tcggtcccca
     1501 cccggtgctg ctgccgattg cgcaagcttg cctcggggcg aggggcaggt cgacgagctg
     1561 ggttgcaacg ctgaaccgtc aaaagccgga cgttgattcg attaccgaaa tgctcgccgc
     1621 gctctatctc gccggccaca atataaattg ggcagcggtg catgcggact cctcatggag
     1681 ccggatcccg ttgcccacat atcccttcca gcgcaagcgt tactggctgg aggacaatgc
     1741 agtccatact gagcgagccc ggaaagttgt cgagcgaatc catcccctgg taggcacgcg
     1801 aacaaatttt gcttccaatg gaccgtgcta tgaagcgcgc tacggcacgc accatgccgg
     1861 cttcctcgcc gatcacaagg ttgcgggaac cgtcgtcctg ccgaccaccg ccgaactgga
     1921 agccgccaca gccgtcggcc gcatgcattt cgggacatcg cggatcagtt tcgacaacgc
     1981 gatgcatcat caggcgatgt cgttcgccaa cggggacgat cggacggtgc gggttttggt
     2041 aactccgctg aattccgaca aggcaagttt caagctcgtg agcaaagatg ccgaggaccc
     2101 gcgggtctgg catacccaca tgacaggcac cttgcgcaag tcagagatga cgagccggcc
     2161 ggcattttca gctaatcaga tccgcgaccg ttgccggcaa acactgtcgg ccgccgattt
     2221 ctatgaccgg ctggacaagc tcgggcttga gtatggaccg ggctttcggg gcgttcggga
     2281 attgtacctc ggtcaacacg agacgctgac gaaagtaagc ctgcctgatg gcctggcaaa
     2341 tacgcaatat ctgatgcacc cggcattcct ggatgcgtgt ctgcatgctt atcctctcgt
     2401 gctcgacggc gtggggaagg ccgggagcga cggccgtcaa tcctacctgc ccatctcgct
     2461 ggaaggatat cgctgctatc aggacgggat cgacaaagcg tgggtgcaca ccaggctgcg
     2521 gagtgtcggg aaagacgata cgcaggtggt cgacattcag gtttacgacg acacggagcg
     2581 cccggtcgcc gacctcgaag gcctcagtgt tcggttgctg ccgctcgaca aggtgcagca
     2641 gccgcaagtc aacgctgacg acttgtttta ccggtcggtc tggcggaaga gcgtcagacg
     2701 gaccggtaaa accggagagg atggcgtccg gaaaagctgg atcatcttcg ccgatgcaag
     2761 aggtgtcgct gcagccttgg ccgacaagat ggaggccgcg gggcatcatt gccacgttgt
     2821 cagcaggggc ggttcattcg cccggcagcg cgcccgagcg tggaccgtca acgagcggcg
     2881 gcctcaggat tttcgtaaat tgctggcgga agtcgcggcg agcgaggcgc taccctgcgg
     2941 tggcctcgtt tacctgtggg gattggatgc tccgtcgata gatggcctga ccctggcaag
     3001 gctgaagagc ggcagcgaga tgatgtgccg cggagcgttg gcgatcttgc atgcgctttc
     3061 tgaaacgcga tcggcaaacc ccgatggcag gcggctctgg tttgtgaccg cgaataccca
     3121 aaatacgcaa gtcggaggcc agtgtgttga tcccgttcaa gcgtgtctgt ggggcctggg
     3181 gcgaaccgct gccatcgagt accccgggac ctggggcgga ttgattgatc tgcaactgaa
     3241 cggtgccccg acccaggaca tcgatgcctt ggcggaagag ttgctgaatc cggatggcga
     3301 gacccagatc gtcatctccg ccggcaaaca gcgccatgtt ccgaggttcg tgcggcaatc
     3361 cctttcagaa ctcgcgctgc agtcgccgcc cgtccgggga gacgcgacgt atttggtgac
     3421 cggcggtttg ggaatgttag ggcgcagcgt cgccaaatgg ctcataggca aaggcgccaa
     3481 acaacttgtc ctgacgggcc gaagcgcaag ccgcgacgcg ataaagaaac tattcagtgc
     3541 ggaagaaatt gacgccgcct caattgaagt cgcggcgtgc gacatcagcc ggcgcgacga
     3601 tgtacgccgt ctcatgcgga caattggcaa gaagtttccg ccagtaaagg gcgtggtgca
     3661 ctcggtcggc gttctggacg acggaatact cgcgcaactt gactgggaca gattctcgcg
     3721 cgtgttcgaa tccaaagtgt atgggagctg gcttctgcac gagtgtacga aatcgctcga
     3781 gctcgacttc ttcattttgc aatcttctgt attgagcgtg cttggttctg ccggccaggc
     3841 aaactacagc gccagcaaca catttgtgga ttccctggca gctcatcgcc gtgttgcggg
     3901 cttgccggcc aaagcgatca actggtcggc ctggtcgggg ggcggactgg cgaccgcctc
     3961 gggcgctcgg ggtgaagcga tgtggtcatc gcttggcgtc aaatttgttt ctcccgatct
     4021 cgcgatggag agctttgaca gactgatgca ccgcgatgtg gatcagattg cagtcgtaat
     4081 tgctgattgg ccaacctatg ccggaaaagt cggcaaccct ccgtttttgg cggagcttct
     4141 gaacgggcgt gaggcccccg gaccgtccgg atctgcgcgg ataaacggtg agccggctgc
     4201 ggcggttgcg cagatggacg gtgggacacg cgcagagctc ctgaacagat tgcaacagcg
     4261 cataatgacg gagctcggct tcgtagatcc catcgatccg gatcggcctc tgaatgaagt
     4321 cgggctggat tcgctgcggt cggtcacgct cgcgaacaac ctggaagacg aattcggcgt
     4381 actgatttcg atttcgcagc tgatcagcgg accgaccatc aatcagcttg tcgatcattt
     4441 atccggtctg tttgcaggga cggaaaaaaa cggggccgct ttggcggccc cggtgccaac
     4501 tctccggaat ggaaaagacc ggtccctgaa tacgctggcc gtgcaactca tcaatgatcg
     4561 cgcatcggac gaggcggtgg cagatccagc tgaagatcga agtggcccgg agatttatcg
     4621 ggaaacggca gcgaacgaaa agatatcgta cggcggcaag ctgaccaacg ggagcggagc
     4681 gcagaatttt gcagcgacct tccttcgccc tgccggagag gccgtatcgc gcggtcgtgg
     4741 aaaatggttg attgcaccgc ggccgaatcc ggatgccagg gcccgtatat tctgttttcc
     4801 ctacgccggc ggaggccttg tatcgtttcg ggcctgggcg caactgttcg atgatgctgt
     4861 ggaggtggtt gcggtcgagg cgccggggcg cgggacgcgc atcgatgaga cagccatcga
     4921 tgacctggat actttcgtcg aaggcttgtt accggaaatg gttggctggc tggacaggcc
     4981 gtccgccttt tttggtcact gcctgggcgg ccttaccatg ttcgcgacac ttcag
//
LOCUS       DASSRL010000248         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1528777, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000248 DASSRL010000000
VERSION     DASSRL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528777"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..907)
                     /locus_tag="VFL49_05085"
     CDS             complement(137..907)
                     /locus_tag="VFL49_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET7911380.1"
                     /translation="MNDSELGRTLAAAAFSRIRADILSSKLLPGTRLRFEPLKKTYGV
                     GLSPLREALSRLVVSGLVTAEGQRGFRVAPASIEDIQDISDVRTNVECLAFRQSIERG
                     DDQWQADVVAAHYRLNLLVERNATEAVDEEVWEARHREFHLALLQACGSRWLLQIVSL
                     LMDQFDRYRRLSVESSLSGKPVSLEHQRIVDAALKRDVDLAVRLLKDHIAHATRLIIE
                     NWDAVVKPRQRGDANHAALNRGTRRTSKAANRSSARSL"
     gene            1156..1353
                     /locus_tag="VFL49_05090"
     CDS             1156..1353
                     /locus_tag="VFL49_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911381.1"
                     /translation="MKFGPTPQNTWDVTPAEVSLIRPVLLPRPASIPALVHRLYGFTA
                     TSGDPIRGLTDSSEVRQAQTS"
     gene            1410..2456
                     /locus_tag="VFL49_05095"
     CDS             1410..2456
                     /locus_tag="VFL49_05095"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911382.1"
                     /translation="MLTSKFLRVAVLCAAAMSFTTAAYAQDPVKIRFILNWKYQGPQA
                     WFFVAQDKGYFKAEGLDVQMDQGEGSGAAVTRVASRAYDVGFGDINALTVFAAKNPND
                     SPKAVFMIYGVSPFSIYSMKDKNITSPKQLEGKTLGAPVDDAPFKLFPAFAKATGIDP
                     KKVTWKHMQPSLREQMLVRGEVDAVSGYVNTVWFSLRKIYKDPGEKVNRMKYSDFGLN
                     LYSNAIIVSKDFLEKNPKAVAGFLRAISKGFQDVLANPSMGVETALKRQPLLDREIEL
                     ENLIATVNVEMLSPESQKIGIGDVDDARLKKGIDVVVNAYELSRTPAPNEVFTREFLP
                     PRDMRNIHEIKQTN"
     gene            2461..3300
                     /locus_tag="VFL49_05100"
     CDS             2461..3300
                     /locus_tag="VFL49_05100"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911383.1"
                     /translation="MNRGSAAAANVMPKRDDADVFVDVRNVKLNYKAGDSSTLALEQT
                     NMMLRRGEFVAIVGPSGCGKSSLLKLASGLRRPTEGEIVVDGSPVLKPIKIVGMAFQN
                     PTMLPWLRTIDNVVMPLKIVEPYRRKFRAEKAKYEQMAASLLTEVGLGNFQQKYPWEL
                     SGGMLQRASLCRALIHDPRLLLLDEPFGALDAFTREDLWQVLQKLWMERNPTVILVTH
                     ELREAVFLADTVHVMSARPGRVLVSHTVDIRRPRTLDDLFTDKASKLVHELRSHISHD
                     RSV"
     gene            3315..4088
                     /locus_tag="VFL49_05105"
     CDS             3315..4088
                     /locus_tag="VFL49_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7911384.1"
                     /translation="MRAWDIAERVFPLLAIVAFFVLWEIACTVFQVPVFILPKPSEFL
                     GDLIRRWPAILPNAVQTLYTTIVGFALGVAVGAAIGACIGLSRFVYRVTYPLLIGFYS
                     VPKIAVVPILVIWFGIGTVPAILTAAIMCLFPVVVNVATGLATTEPELEDVLRALGAS
                     KFDILRRVGLPRSMPYFFASLKIAISLAFVGTVVAETVAANIGIGSMMILASSTFDVP
                     LVFAGVFLLAVLGVAMYAIFALIESHVTFWARRNADIAA"
     gene            complement(4246..4908)
                     /gene="petA"
                     /locus_tag="VFL49_05110"
     CDS             complement(4246..4908)
                     /gene="petA"
                     /locus_tag="VFL49_05110"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET7911385.1"
                     /translation="MADVPSEFDAEHTGLSEAKTRRDFIYIATAAVGAVGAAAALVPL
                     IAQMNPDASTLAAGGPIELDLAQVQPGQQVVVRWRSRPIFVTHRTPEILRMLRNPQLL
                     ARLADPHSAEVQQPSYADNWHRSVKPEFGVLVGICTHLGCIPMFEPTPNATAPAPNWP
                     GGYFCPCHGSKYDLAGRVFQGVPAPYNLPVPPYHFVTDTKIRIGENPKNVSFDFSSIR
                     QI"
BASE COUNT         1007 a         1489 c         1542 g         1047 t
ORIGIN      
        1 gttcatcggc accatgatcc gcgaaggttt tctcccgcct cagcagaaag acatgctgat
       61 cgtcgatgcc gatccgcgcg tcctgctcgc gcgctgccgg acgttcacgc cggtaacggt
      121 cccgaagtgg atgtgactac aggctgcgcg cggacgaacg gttcgcggct ttgcttgtgc
      181 gccgcgtgcc gcggttgagg gcggcgtggt tggcgtcccc gcgctgcctc ggtttcacga
      241 cggcgtccca gttctcgatg atcagccgcg tcgcgtgcgc aatgtgatcc ttcagcagcc
      301 ggaccgcaag atcgacatcg cgcttgagcg cggcgtcgac gatccgctgg tgttcgagcg
      361 ataccggctt gcctgacagg ctgctttcga cggagaggcg gcggtagcgg tcgaactggt
      421 ccatcagcaa ggatacgatt tgcagtagcc agcgcgaccc gcaggcctgc aggagcgcga
      481 ggtgaaattc gcgatggcgc gcttcccaca cctcctcgtc gacagcctcg gtcgcgttgc
      541 gctcgaccag caggttgagc cggtagtgcg ccgcaacgac gtccgcctgc cactggtcgt
      601 ccccacgctc gatcgattgg cggaaggcca gacattccac gttggtacgg acatcggaga
      661 tgtcctggat gtcctcgatc gacgccggcg caacgcggaa accgcgctgt ccttcggcag
      721 tgaccagtcc gctgacaacc agccgggaca atgcctcgcg cagcggactc aagccgacgc
      781 cataggtctt cttcagtggc tcgaacctta gtcgcgtgcc gggcagcagc ttagagctga
      841 ggatatcggc gcggatacgc gaaaacgccg cggccgcgag cgtgcgtccc agttcagaat
      901 cgttcatcgg aaatggtccg gcatagagaa ggggagactg gcggcgttgt gggcattctg
      961 attagaaaat cggcagttgt taggactaat ctccgtaagt gctatattct acactatctc
     1021 agataatttc cagaggttcg aaattttgtt gacgtcttta cccgcctttg gtccaatctt
     1081 caggtgggtc aacaccggcc cgttcggcgc ctgccggccg gcttgcgggg ttgagtgtgt
     1141 ggaggcgagg tctccgtgaa gtttggtccg acgccgcaga acacctggga cgttacgccg
     1201 gccgaagtca gtttgatccg cccggtacta ttgccgcggc cggcctcgat tcccgctctc
     1261 gtgcatcggc tctacggctt cacggcgacg agcggcgatc ccatcagggg gctgacggac
     1321 agcagcgaag tgcggcaagc tcaaacgtca taggactgga agaacgcgtg caaccggcca
     1381 tgcagccgga aatccagggg aggactcaga tgttgacatc aaaattcctg cgcgtggctg
     1441 tgctgtgtgc cgccgccatg tcgttcacaa ccgctgctta tgcccaggat ccggtcaaga
     1501 tccgcttcat cctcaattgg aaatatcagg gaccacaggc ctggttcttc gtcgcccagg
     1561 acaagggtta cttcaaggct gaaggactgg acgtccagat ggatcaaggc gagggatcgg
     1621 gcgccgcggt cacgcgggtt gcgagccgcg cctacgatgt gggcttcggc gacatcaacg
     1681 cgctcacagt gttcgccgcc aagaacccga acgattctcc gaaagcggtg ttcatgatct
     1741 acggcgtttc acccttctcg atctactcga tgaaggacaa gaacatcacg tcgccgaagc
     1801 agcttgaggg caagactctc ggcgctccgg tcgatgacgc gccgttcaag ctgttcccgg
     1861 ccttcgccaa ggcgaccggc atcgatccca agaaagtgac gtggaagcac atgcagccca
     1921 gcctgcgcga gcagatgctg gtgcggggcg aagtggatgc ggtctccggc tacgtcaaca
     1981 cggtgtggtt cagcctgcgc aagatctaca aggatccagg cgagaaggtg aaccggatga
     2041 aatacagcga cttcggcctg aatctctaca gcaatgcgat catcgtttcg aaggacttcc
     2101 tcgagaagaa tccgaaggcg gtcgccggtt tcctgcgcgc gatctcgaaa ggcttccagg
     2161 atgtgctcgc aaatccgagc atgggtgtcg aaaccgcgct caagcgccag ccgctgcttg
     2221 accgtgaaat cgaactcgag aacctgatcg cgacggtgaa tgtcgagatg ctgtcgccgg
     2281 aatcgcagaa aatcggcatt ggtgatgtcg atgatgcgcg gctgaagaaa ggcatcgacg
     2341 tggttgtcaa cgcctatgag ctcagtcgga cgcccgcgcc gaacgaggtg ttcacccgcg
     2401 aatttctgcc gccgcgcgac atgcgcaaca tccacgaaat caaacagacc aattgacgta
     2461 atgaacagag gaagtgccgc cgcggccaac gtgatgccga aacgcgacga tgccgacgtg
     2521 ttcgtcgatg tcagaaacgt aaagctcaat tacaaggctg gcgacagctc gacgctcgcg
     2581 cttgagcaga ccaacatgat gctcaggcgc ggcgaatttg tcgccatcgt cggcccgagc
     2641 ggctgcggca agtcatcgct cctgaagctt gcttccggcc tgcggcggcc gactgaaggc
     2701 gagattgtcg tcgacggcag cccggtcctc aaaccgatca agatcgtcgg gatggctttc
     2761 cagaatccga cgatgctgcc gtggctgcgg acgatcgaca acgtggtgat gccgttgaag
     2821 atcgtcgagc cttaccgccg caaattccgc gcggaaaaag ccaagtacga gcaaatggcg
     2881 gcctcgctcc tcaccgaggt cggactcggc aattttcagc aaaaataccc ctgggagctg
     2941 tcaggcggaa tgttgcagcg cgcgtcgctc tgccgcgcgc tgatccatga cccgcgcctg
     3001 ctgctgctcg acgagccgtt cggcgcgctc gatgcgttca cccgcgagga tctgtggcag
     3061 gtgctgcaaa agctatggat ggagcgcaac ccgacggtga tcctggtcac gcatgaactg
     3121 cgcgaggcgg tattccttgc cgacaccgtg cacgtcatga gtgcgcggcc gggccgtgtg
     3181 ctggtgtcgc acacggtaga catcaggcga ccgcgcacgc tcgatgacct cttcaccgat
     3241 aaagcctcga agctcgtgca tgaattacgc agtcacattt cccacgaccg cagcgtctga
     3301 aaggagaatg agaaatgcgc gcatgggata tcgccgagcg ggtgtttccg cttcttgcga
     3361 tcgtcgcgtt cttcgtcttg tgggaaatcg cctgcaccgt tttccaggtt ccggtcttca
     3421 ttctgccgaa accgagcgag tttctcggcg atctcatccg gcgttggccg gcgattctgc
     3481 cgaacgccgt gcagacgctt tatacaacca tcgtcggctt cgcgttgggc gttgcggtcg
     3541 gtgcggcaat cggcgcctgc atcggactct cgcgcttcgt ctatcgggtg acgtatccgc
     3601 tgctgatcgg cttctatagc gtgccgaaga tcgcggtggt gccgattctg gttatctggt
     3661 tcggcattgg tacggtgccg gcgatcctga cggccgcgat catgtgcctg ttccctgtcg
     3721 tcgtgaacgt tgcgaccggc ctcgccacta cggaaccgga gctggaagat gtgctgcgcg
     3781 cgctgggcgc gagcaagttc gacattcttc gccgggtagg gttgccccgg tcgatgcctt
     3841 atttctttgc ctcgctgaaa atagcgatct cgcttgcctt cgtcggcacc gtcgtcgcgg
     3901 agacggtggc ggcgaacatc ggcatcggca gcatgatgat tctggcaagc tcgacattcg
     3961 atgtgccgct ggttttcgcc ggcgtgttcc tgctcgccgt gttgggcgtc gcgatgtacg
     4021 cgatctttgc cttaatcgaa agtcacgtca ccttctgggc gcgtcgcaac gcagacattg
     4081 ccgcgtagag accagactgc gctgcaattg ggttagcgtt gcgacttgct gcgcacctgg
     4141 cgtcgcacgg cgctacgcca ggcgcacagt gcacgctcaa cgcttgatga cgtccgcaat
     4201 tttttagtca cgcggcggat ttgtcgcatg ttcgcgcaac aatttttata tctgtcgaat
     4261 cgacgagaaa tcgaagctta catttttcgg attttctccg atgcggattt tcgtgtccgt
     4321 cacgaaatga tagggcggta ccggtaggtt gtaaggtgca gggacgccct gaaatacgcg
     4381 accagccaga tcatatttcg acccatggca tggacaaaaa tagccgcccg gccaattcgg
     4441 agccggggcg gtcgcattcg gcgtcggctc gaacatcgga atgcagccaa gatgagtgca
     4501 tattcccaca agcacaccaa attcgggctt caccgagcga tgccagttat cggcatagga
     4561 aggctgttga acttccgcgg aatgcgggtc tgccaatcgt gccagcaact gcgggtttct
     4621 gagcatcctc agtatttccg gcgttcggtg ggtgacaaat atcggtctgc ttcgccagcg
     4681 aacgacgacc tgctgtccgg gttggacttg tgcgaggtcc agctcgatag ggccgccggc
     4741 tgcgagggtc gaagcatccg ggttcatctg ggcgatcaag ggcacgagag ccgccgctgc
     4801 gcctacggcg cccaccgccg cggtcgcaat gtaaatgaaa tcacgccgcg tttttgcttc
     4861 agaaagcccg gtatgttcgg catcaaattc tgacggtaca tcggccaaga caagctccct
     4921 cgtgtccaca aggtcgcccg tcaccgattt actattacgg catcagccgc aatctcatct
     4981 gccttttata taggacacga ctccgctatc accagatcgc gtcgtgagat tcgattaaat
     5041 cgaaagacac atgaagtacg cgaccaaaaa accgacatct gtttg
//
LOCUS       DASSRL010000249         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1336279, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000249 DASSRL010000000
VERSION     DASSRL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336279"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VFL49_05115"
     CDS             complement(<1..815)
                     /locus_tag="VFL49_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HET7911386.1"
                     /translation="MKDDQGLTQLIGDIYDAALDSSRWTSVLAKIAGFAGGQAAGLLS
                     KDSVSKSGSIHYQSGVDPYFVRLYAETYWKFDPVASLPDCDLEQIVSVPDLVSYDEFR
                     AGRFYREWAQPQGWVDAANAVLEKSATSCAYLSVLRNDASGMVDEEMRRRMRLIVPHV
                     RRAVRTGRVVDLSQAEAATFADILDGLSPAIYLIDADGLIVHGNAAGRAVLDKGDLLR
                     AVHGRLTAIDTQIDQALHAAFLAANKDAETGGKGSALPMRARAGERYVAHVLPL"
     gene            1532..2749
                     /locus_tag="VFL49_05120"
     CDS             1532..2749
                     /locus_tag="VFL49_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7911387.1"
                     /translation="MQTPSKFAQLSVLTLAFALSQAFRTVPSITVNGIADELHAGPAA
                     LALFGGVFHWSFALLQVPVGLALDIFGVRRTVIVLSGFAVLGGAICTLTPDVNWLFLG
                     QILIGIGCAPAFMAAIVFTSRHWPAGRFAAISGFVLAIGSGGMLLSATPLAWFIERWS
                     WRSGFAILMAMSILTLFACALVLEKSAAKPSRRLTAEILAGFYGLRFVLFGRRPIALL
                     AIGFVSYGAVITIRGLWIVPMFVERYGLTLIAAGNIALLVSVAMIVGPAVAGHFDPGD
                     KARPAAITAIAVLMTATTGALALFDGHSVWLDAALCIFLGFISGFFILVYAEARSSYP
                     AELTGRGLTALNMSFFLGAAVAQSLSGVIAAAAQRAGWNAIDAVLFFLTAVLLVGTIC
                     FFTLIRPAKPRSA"
     gene            complement(2842..3399)
                     /locus_tag="VFL49_05125"
     CDS             complement(2842..3399)
                     /locus_tag="VFL49_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HET7911388.1"
                     /translation="MEIRRTLIVKDTVSFEGGAPAIVPVTRVAACALVANPLAGVVTD
                     DLQALIPFGADLGAYLVRAAMEALQAPSISYGKAAIVGTAGDIEHAAAIIHPRMGKPM
                     REAIGGGQAIIPSNVKVAGAGASIDVPLGHKDDVWSFDEIDTLTITIPGAPRPEEIVV
                     IVALANGGRPRPRVAKSGAVLPKRV"
     gene            complement(3404..>3789)
                     /locus_tag="VFL49_05130"
     CDS             complement(3404..>3789)
                     /locus_tag="VFL49_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7911389.1"
                     /translation="INILKMICSGFLDRWPRLKLVCAHTGAFSLVLRARMQREVDTNA
                     QLSAALKAPVGDYLGRLYFDTVCFEPGILQYAASVVPVEHLLMGSDAPFPLGEPEPVK
                     FVRKALPANQAETIMQKNFDDLVGG"
BASE COUNT          717 a         1158 c         1117 g          797 t
ORIGIN      
        1 aggggcagaa cgtgcgcaac ataacgctcg ccggcacgcg ctctcatcgg aagagcgctg
       61 cctttacccc cggtttccgc atccttgttt gcggcgagga aagctgcatg aagcgcctgg
      121 tcgatctggg tatcgatcgc cgtcaaccgc ccgtggacgg cacgaagcag gtcgcctttg
      181 tcaagcacgg cgcggcccgc cgcattgccg tgcacgatca gcccatcagc gtcgatgaga
      241 taaatcgcgg ggctaagacc gtcgagaata tcggcgaacg tggcggcttc tgcctggctg
      301 agatcgacga ccctgccggt gcgaaccgca cgccggacat gtggcacgat gagacgcatg
      361 cggcggcgca tctcttcatc gaccatgccg ctcgcgtcgt tacgcaatac gctgagatac
      421 gcacagctcg ttgcggattt ttcgaggacg gcgtttgccg catcgaccca gccctgcggc
      481 tgtgcccatt cacggtagaa gcggcctgct cgaaattcat cataggaaac gagatccgga
      541 acgctgacga tctgttcgag gtcgcagtcg ggcaagctgg caacaggatc aaatttccaa
      601 taagtctcgg cataaagccg cacgaagtag gggtcgactc ccgactgata gtggatgctg
      661 ccggacttac tcaccgaatc cttggataac agtccagccg cctgcccacc cgcaaatccg
      721 gcgatctttg cgagcacgga tgtccaccgc gacgaatcga gcgccgcgtc gtagatatcg
      781 ccgataagtt gcgtgagacc ttgatcgtct ttcatgtcac ccagcgcgcc gacttcccga
      841 ccgggcgctg acacagcgat gcccccggtg cagatacgac tcagccggtg cgaccgctcg
      901 gccggtccag ctgcacgaga ggtaatctac gacattcccc tggagacttc ttggacgaga
      961 gcgcaaatga cttggcctac agcgcgcgat gcaacgcgtt aagcttcggt cgaaccaata
     1021 tgcgtttcag cttttcagcg tgatcgcaca cattcaaaaa accggcgctg ctggccgcgc
     1081 cgaaacgggc ggcgaacgcg ccgctataca cgcgtccgat cgaatttcgc tatgtcctga
     1141 atactggtga gggtgagacc catagcagat gcgtatagcg cgcgatgaca aaccgccgtg
     1201 cgaatgcaaa tgagtttgag tgctatcctg ataccttttc caattgtttt ccgatcagct
     1261 tcctgcttca ccttatcgca cggaaaattt ccctctcagg cgcgacgagt accggcgggt
     1321 cgaaaactgc tgcgaggaaa acgctggccc ggaccgtgca ctcaaagcag ctaccgggcg
     1381 atcacaccag ctgcgtaacg acatttgcag gcgttgtggc gaatgaattg caaaggatac
     1441 tcgcgggcag gaattccact tgaatgcgat agcgcccggc agcgaattcc attattggat
     1501 cgcgcgaggc gaacagaatc tccgctccac aatgcagact ccatcgaaat tcgcacagct
     1561 atcggtgctg acccttgcct tcgcattgag tcaggccttt cgcaccgtcc cgtccatcac
     1621 ggtcaacggg atcgctgacg aattgcacgc cggcccggcc gcccttgctt tgttcggcgg
     1681 ggtttttcac tggagttttg cactgttgca agtgccggtt gggctcgcgc tggacatttt
     1741 cggagtgcgc cgcaccgtga ttgtgctgtc cggatttgca gttttgggcg gagcaatttg
     1801 tacgctgact cccgacgtga actggctttt cctcggccag atcctgatcg ggataggctg
     1861 tgctccggct ttcatggctg cgatcgtgtt tacgtccagg cattggcccg cggggcgttt
     1921 cgccgccatc tccgggtttg ttttggcgat cggcagcggc ggaatgcttc tgtcggcgac
     1981 gccgcttgcg tggttcatcg aacgctggtc gtggcggagc ggcttcgcaa tcttgatggc
     2041 gatgtcgatc ctcactctgt tcgcatgcgc gctcgtcctt gagaaatcgg cggccaagcc
     2101 ttcgcggaga cttacagccg agatcctggc cggcttttac ggtcttaggt ttgtcctgtt
     2161 cgggcgccgc cccatcgcgc ttcttgctat tggatttgtg agttacggag ccgtcatcac
     2221 catccgcggc ctgtggattg tgccgatgtt cgtcgagcga tatggactga cgctaattgc
     2281 ggcaggcaac atcgccctgc tggtttcggt cgcgatgatt gtcgggcctg cggtggcggg
     2341 ccacttcgac ccaggagaca aggcgcgtcc cgccgccata accgccatag cggtattgat
     2401 gacagccacg accggcgcgc tggctctatt cgatggtcat tcagtttggc tcgacgctgc
     2461 cctctgcatt ttcctgggtt tcatttcggg tttctttatc cttgtctatg ccgaggcgcg
     2521 cagttcgtat cctgcagagc ttacgggacg cggactgacg gcgctcaaca tgagcttttt
     2581 tctcggagcc gcggtggctc aatcgctctc gggcgtgatt gccgctgccg cgcaacgcgc
     2641 gggctggaac gcaattgacg ctgtgctgtt ttttctgact gcggtgctgc ttgtcgggac
     2701 aatctgcttt ttcacgctga ttcggcccgc caagccgcgc tcggcctgag ctaatccaaa
     2761 cgccagtcgt ccgtgccaaa ggccgatgaa gcggtcttgt tcgccgcact catacgcaaa
     2821 ccgcgacgtg cccttccccc gtcagacccg cttcggaaga accgccccgg acttggccac
     2881 ccgtgggcgc ggccggccgc cgttcgccaa tgccacgatc actacaattt cctcggggcg
     2941 cggcgcgccc gggatcgtga tcgtcagcgt gtcgatttcg tcgaaagacc agacatcgtc
     3001 cttgtgcccc agtggaacgt cgatcgaagc cccggccccg gcgaccttga cgttggacgg
     3061 aatgatcgcc tgcccgccgc cgattgcctc gcgcataggc tttcccatcc ggggatgaat
     3121 gattgcagct gcgtgctcga tgtcccctgc cgttcccacg atcgcagcct tgccgtaaga
     3181 gatggacggc gcctgcaggg cctccatggc agcgcggacc aggtaggcgc cgaggtcggc
     3241 tccgaaagga atgagcgcct ggaggtcgtc ggtcacgaca ccggcaagtg ggttggccac
     3301 cagcgcgcag gcggcaacgc gtgtgacagg cacgatcgcg ggggcgccgc cctcgaagct
     3361 gacagtgtct ttcacgatga gcgttcgccg gatctccatt atctcaacct ccgacaagat
     3421 cgtcgaaatt tttctgcatg atggtctcgg cctgatttgc cggaagcgct ttgcgcacga
     3481 acttcacggg ctccggctcg cccaacggaa acggtgcatc gctccccatc aaaagatgct
     3541 ccaccggaac gacgctggcg gcatattgaa ggatgccagg ctcaaaacag accgtatcaa
     3601 aataaagacg cccgagataa tcgccgacgg gcgccttcaa agcggcggac agctgggcat
     3661 ttgtatcgac ctcccgctgc atgcgcgccc gcaaaaccag gctgaacgcg ccggtatgag
     3721 cacagaccaa tttcagccgc ggccagcgat cgaggaaccc ggagcagatc atcttgagga
     3781 tattgatgg
//
LOCUS       DASSRL010000250         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1441455, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000250 DASSRL010000000
VERSION     DASSRL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441455"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFL49_05135"
     CDS             complement(<1..545)
                     /locus_tag="VFL49_05135"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET7911390.1"
                     /translation="MNRQLVRLGLGLIASALGSAPAFAHHLMGGRTPATFGEGILSGL
                     GHPVIGLDHLTAVVAVGCLAAAHRAVSALAIAFVLAMVGGVVLHLHGTTVPGAEILVA
                     LTVLALGVLIVLHRDMPASAAVALFAIVGVIHGYALGESIYGAERTPLYAYLIGLAVI
                     QSVIALAAAHVTRGLVRRSPD"
     gene            641..1471
                     /gene="dapD"
                     /locus_tag="VFL49_05140"
     CDS             641..1471
                     /gene="dapD"
                     /locus_tag="VFL49_05140"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET7911391.1"
                     /translation="MANDKLASTIDDAFEARDKIGPKTKGAVRKAVNAALELLDAGKA
                     RVAERKPDGQWQVNQWLKKAVLLSFRLNDLSVIPGGPDKAVWWDKVDSKFKGWSAARF
                     RKAGFRAVPGCVVRRSAFIAPGVVLMPSFVNLGAYVDSGTMIDTWATVGSCAQIGKNC
                     HISGGAGIGGVLEPLQAGPVIIEDNCFVGARSEVAEGVIVRTGAVLSMGVYLGASTRI
                     VDRETGQSTQGEVPPYSVVVPGTLPGKSGGPGLYCAVIVKRVDERTRSKTSINELLRD
                     "
     gene            1481..2638
                     /gene="dapE"
                     /locus_tag="VFL49_05145"
     CDS             1481..2638
                     /gene="dapE"
                     /locus_tag="VFL49_05145"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET7911392.1"
                     /translation="MSADPIAIARDLVRCPSVTPAEGGALAFLEGILKKAGFAVHRMT
                     FREAGAEDVENLYARIGTASPNLVIAGHTDVVPPGDEKAWKHPPFSGEVADGEMYGRG
                     AVDMKGGIACAVAAVLDHLGQRGGKPKGSISFLITGDEEGIAVNGTPKLLKWTAERGE
                     KFDHCILAEPSNQDELGDTIKIGRRGSLNGHLIVSGTQGHVAYPQRASNPIRGLVTLV
                     SALQAEPLDQGSAQFAPSHLEFTSVDVGNKTVNLIPGEARARFNIRFNDKHTLDSLKK
                     LIEQRAAKAAGGKIKFAFQWESSNAGVFVTKPGPFTDLVATAVAEETGRKPALSTTGG
                     TSDARFITHHCPVVEFGLVGQTMHQVDERVPIADLQKLTAIYRKIIDRYFA"
     gene            complement(2652..3389)
                     /gene="truA"
                     /locus_tag="VFL49_05150"
     CDS             complement(2652..3389)
                     /gene="truA"
                     /locus_tag="VFL49_05150"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7911393.1"
                     /translation="MPRYKLTVEYDGRPFAGWQIQVGQLTVQGLLTSAVEALSGDKTL
                     VQGAGRTDAGVHARAQVAHVDLSKDWDTDTIRDALNAHLRPNPIAVLAAERAANDFSA
                     RTSATRRHYLYRIINRRADLTLDAGRAWRVPRPLDATAMHAAAQRLIGKHDFTTFRST
                     ECQAKSPVKTLDSLKIERDGEDVYVTSVARSFLHNQVRSMVGSLVVVGEGKWSPDDLA
                     KALDARDRAACGPVAPPDGLYLMRVDY"
     gene            complement(3391..>3768)
                     /locus_tag="VFL49_05155"
     CDS             complement(3391..>3768)
                     /locus_tag="VFL49_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7911394.1"
                     /translation="LAAAERGSLTLTPQPDAGITYAEKVSKGETRVDWSKPWKQVHDH
                     IRGLSPFPGAWFEIDGVRIKALRSAKGEGSGAAGAVLDNKLTIACGAGAVRLTQVQRA
                     GKQPMSADEFLRGTPVKSGARAA"
BASE COUNT          725 a         1233 c         1207 g          603 t
ORIGIN      
        1 aaatcgggag atcggcggac aagcccgcgg gtcacatgcg ctgcggcgag cgcgatcacg
       61 ctttggatga ctgcgagccc gatgagatag gcgtaaagcg gtgtccgctc ggccccgtag
      121 atcgattcac ccagggcata accgtggatc acgcccacga tcgcgaacag cgccaccgcg
      181 gcgctcgccg gcatgtcgcg gtgaagcact atcagcacgc caagcgccag gacagtcaat
      241 gcgacgagga tctcggcgcc cggcaccgtc gtgccatgca ggtgcaggac aactccgccc
      301 accatcgcca gcacgaaagc aatcgccaaa gccgataccg cgcgatgtgc ggctgccaga
      361 cagccgacgg cgacgaccgc ggtcaggtga tcgaggccga tcaccggatg gcccaatccc
      421 gacaggatgc cttcaccaaa cgtcgccggc gtgcggcccc ccatcaggtg atgcgcgaaa
      481 gccggtgccg aaccgagtgc cgatgcgatg agacccaaac ccagacgtac caattggcga
      541 ttcattgctt ttctcccggc acgaccatag ctcgcccggc gcggcttgac atcccctgcc
      601 ccggccccga caaaccgcag ccgaacaagc cggagcatcc atggccaacg acaagcttgc
      661 cagcaccatc gatgacgcct tcgaggcgcg cgacaagatc ggcccgaaga ccaagggcgc
      721 cgtccgcaag gcggtgaacg ccgcgctgga gctgctcgat gccggcaagg cgcgcgtggc
      781 cgagcgaaag cccgacggcc aatggcaggt caaccagtgg cttaagaaag cagtgctgct
      841 gtccttccgg ctcaacgacc tgagcgtgat cccgggcggg ccggacaaag ccgtgtggtg
      901 ggacaaggtc gattccaaat tcaagggctg gagcgccgcg cgctttcgca aggcgggctt
      961 ccgcgccgtt cccggctgcg tggtccgccg ctcggccttc atcgcgccag gcgtcgtgct
     1021 gatgccctcc ttcgtcaacc tcggcgccta tgtcgattcc ggcacgatga tcgatacctg
     1081 ggcgaccgtc ggaagctgcg cgcagatcgg caagaactgc cacatctccg gtggcgccgg
     1141 cataggcggc gtgctggagc cgctgcaggc cgggccggtt atcatcgagg acaattgctt
     1201 cgtcggggct cgctccgaag tggcggaggg cgtcatcgtg cgcaccggcg cagtcctgtc
     1261 gatgggcgtc tatctcggcg cctcgacccg cattgtcgac cgcgagacag ggcagagcac
     1321 gcaaggcgaa gtgccaccct actccgtggt tgtacccggc actttgccgg gaaaaagcgg
     1381 cggtccgggg ctgtattgtg ccgtcatcgt caagcgcgtc gacgagcgta cgcgttcaaa
     1441 gacaagcatc aacgagctgc tgagagactg aaggccgacc gtgtccgccg accccatcgc
     1501 catcgcccgc gatctggtgc gctgcccctc ggtgacaccg gcggaaggcg gcgcattggc
     1561 gtttctcgaa ggcatattga aaaaagcggg cttcgcggtg caccggatga cgttccgcga
     1621 ggccggcgcc gaggacgtcg aaaacctcta cgcgcgcatt ggcactgcct cacccaacct
     1681 ggtcatcgca ggccacactg acgtggtgcc accgggcgac gaaaaggcgt ggaaacatcc
     1741 accgttttcc ggcgaagttg ccgacggcga gatgtacggc cgcggcgcgg tcgatatgaa
     1801 aggcggcatc gcctgcgccg ttgcggcggt gctcgatcat ctcggccagc gcggcggaaa
     1861 gccgaagggc tcgatctctt tcctgattac cggcgacgaa gagggtatcg ccgtcaacgg
     1921 cacgccgaaa ttgctgaagt ggacggccga gcgcggcgag aagttcgatc actgcatcct
     1981 ggccgagccg agcaatcagg atgaactcgg cgacactatc aagatcggcc ggcgcggctc
     2041 cctcaacggc cacctcatcg tgagcggtac gcaagggcac gtggcctatc cgcagcgcgc
     2101 cagcaatccg atccgcggcc tggtcacgct ggtttccgcg ctccaggccg agccgctcga
     2161 ccagggcagc gcacaattcg cgccatcgca tctcgagttc acatcggtcg acgtcggcaa
     2221 caaaacggtc aatctcattc cgggcgaagc acgggcacgc ttcaacatcc gtttcaacga
     2281 caagcacacg ctcgactcgc tgaaaaagct gattgagcag cgcgcggcga aggcagccgg
     2341 tgggaagatc aagttcgcgt tccagtggga gtcgtccaat gccggcgtgt tcgtgaccaa
     2401 gccggggccg ttcaccgacc tggtcgccac cgctgtcgca gaagaaaccg gccgcaagcc
     2461 ggcgttatcg accaccggag gcacgtcgga cgcgcgattc atcacgcatc attgtcccgt
     2521 ggtcgagttc ggcctggtcg gccagacgat gcatcaggtc gatgagcgcg tgccgattgc
     2581 cgacctgcaa aagctcaccg cgatctaccg gaaaatcatc gacagatatt tcgcctgacc
     2641 gcgagggcgg gtcagtagtc cactcgcatc agatagagcc cgtccggcgg agcgacagga
     2701 ccgcaggccg cgcgatcacg cgcatcgagc gccttcgcaa ggtcgtcggg gctccatttg
     2761 ccctcgccga ccacgacgag cgagccgacc atcgagcgca cctgattgtg caggaacgag
     2821 cgcgcgaccg aggtcacata aacgtcctcg ccatcgcgct cgatcttgag actatcgagc
     2881 gtcttcaccg gtgatttggc ctgacattcg gtcgagcgaa acgttgtaaa atcgtgcttt
     2941 ccgatcaggc gttgcgccgc ggcgtgcatt gcagtcgcat caagcgggcg cggcacgcgc
     3001 caggcacggc cagcatcgag cgtaaggtcg gcgcggcgat tgatgatgcg gtagagatag
     3061 tggcgcctgg tcgccgatgt ccgggcactg aaatcattgg cggcacgttc ggccgcgaga
     3121 accgcaatcg ggttcggccg caggtgcgca ttgagcgcgt cgcgtatggt gtcggtatcc
     3181 cagtccttgg aaagatcgac gtgagcgacc tgcgcacgcg catgcacacc ggcgtcggtg
     3241 cggccggcgc cttgcaccag cgttttatcg ccggagaggg cttcaaccgc ggacgtgagc
     3301 agcccctgaa cggtgagctg gccgacctga atttgccagc cggcgaacgg ccgcccgtca
     3361 tattcgactg tcaacttgta gcgcggcata tcaggcggcc cgcgcgccgg atttcaccgg
     3421 cgtaccgcgc aggaattcgt cagccgacat cggctgtttg ccggcgcgct gcacctgcgt
     3481 gagacgcacc gctcccgcac cgcatgcgat ggtgagcttg ttatcgagca ccgcgccggc
     3541 agcaccggaa ccctcgccct tcgccgagcg caacgccttg atccgcacgc catcgatttc
     3601 gaaccaggcg ccggggaacg gcgaaagccc gcggatgtga tcatgcacct gcttccatgg
     3661 cttcgaccag tcgacgcgcg tctcgccttt ggaaaccttt tcggcgtaag tgatgcccgc
     3721 gtccggttgc ggggtgagcg tgagcgaacc acgctcggct gcggcgag
//
LOCUS       DASSRL010000251         6964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_59148, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000251 DASSRL010000000
VERSION     DASSRL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59148"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VFL49_05160"
     CDS             complement(<1..1533)
                     /locus_tag="VFL49_05160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7911395.1"
                     /translation="MTESLDLSLYAKPMADGTLGMELAVEGIACGGCIGRIERAVRNV
                     PGVTEARLNFTNRRLHVVWSPGLAEPAAILRALEDGGYHGHPFVPMRAEQQEAAEARR
                     LTRCLAVAGFAAMNIMLLSVSVWSGNVADITPETRDFFHWASALIALPAAAYAGRPFF
                     ASAWRALKARSLNMDVPISLGVILALGMSLVETANHAEHAYFDSAIMLLFFLLVGRAL
                     DHAMRRKTRAVASNLAALKAENAHRLIGDELVSVPSAALKAGDRVLVKPGERVPADGM
                     VLGGKSDLDESLITGETAHRNVASGATVYAGSMNYSGALTLKVTAAGDAGLIDEIEKL
                     LEKAASGKSRAMRLADRASRVYAPMVHLGAALTLAGWLIAGASLHDAIITAIAVLIIT
                     CPCALALAIPAVQVVTSGALFRSNVILNAGDAIERLAEIDTVIFDKTGTLTLPEPRVV
                     TTVALDPKLLQMAARLALSSRHPLAVALAREATIRVPFDEVVEEPGQGVRAHIDGIET
                     RLG"
     gene            complement(1530..2030)
                     /locus_tag="VFL49_05165"
     CDS             complement(1530..2030)
                     /locus_tag="VFL49_05165"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET7911396.1"
                     /translation="MRTSRKPRELTGKHVLICFVGFFGLVFAVNAVMVKAAMSTFGGV
                     QTTSSYKAGLMFGQDLARAEKQDALHWQVSGRLSRDDAGNAVLDISVRDTRDVPVKGL
                     AAEARLAHPADERRDHLIALNDASGGRFHGVTRAEPGQWELIVDLYKDDARVFRSRSR
                     VMLNSP"
     gene            complement(2044..3564)
                     /gene="ccoG"
                     /locus_tag="VFL49_05170"
     CDS             complement(2044..3564)
                     /gene="ccoG"
                     /locus_tag="VFL49_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HET7911397.1"
                     /translation="MTAHVIMSRDQPAPGAPALAHLVKEAEVGGAPPEDDVPLYAPRR
                     NIYPQSVHGTFRNIKWAVLVVTLGIYYLLPFVRWDRGPNAPSQAVLIDFPNRRFYFFF
                     IEIWPQEIYYLTGLLILAAIVLFLMNAVAGRIWCGYLCPQTVWTDLFFAIERFVEGDR
                     REHMQRDARKWTAGTYARKGAKHFLWLMVAWWTGGAWVLYFSDAPTLVKDLATGEAPL
                     IAYIWIGILTVTTYSLAGHMREQVCLYMCPWPRIQAALTDEYALNVTYRYDRGEPHCS
                     VKKAEQLRSQGQPAGDCIDCLQCVHVCPTGVDIRGGANLGCIQCGLCIDACDAIMSKL
                     GRPTRLIAYDTDLNIKQRIEGKKPFYKLVRMRTALYAGIIAVVGGIMLYTLATRDSEG
                     ISVIHDRNPMYVRLSDGAIRNAYTIRIVNKRLKYRDFIMSVDGLPSTLIDFVGLPPRA
                     DGRQLITIGPDQTKEVRVVVTDYSPTPPPPSTSILFKLIDIDSGETAEMRDHFFGP"
     gene            complement(3561..4439)
                     /gene="ccoP"
                     /locus_tag="VFL49_05175"
     CDS             complement(3561..4439)
                     /gene="ccoP"
                     /locus_tag="VFL49_05175"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HET7911398.1"
                     /translation="MVEEHKEIDAVTGTPTTGHEWDGLRELNTPLPRWWLWLFYITII
                     WSIGYWIVYPAWPLFTNSTQGLFGYHTRTALFEDLDALKQQRGPMMDKLAGASLAEIA
                     ADPQLLDFARAQGRVAFADNCAPCHGAGGGGAKGYPNLNDDEWLWGGKLADIEQTIRY
                     GARASDNKGRTGSMPAFGRDKILKPNEISAVADFARSLSGLSTEPKADLALGKKVYAD
                     NCAVCHGDDGKGNREMGAPNLTDKIWLYGSDKATIVQGIENGRGAVMPNWGGRLSEPT
                     IKALTVYVHTLGGGEK"
     gene            complement(4442..4597)
                     /locus_tag="VFL49_05180"
     CDS             complement(4442..4597)
                     /locus_tag="VFL49_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HET7911399.1"
                     /translation="MDPTYRAIAEFAQTWGLAYFVVVFLLVLVYALWPSRKKKFDEAA
                     RIPLRED"
     gene            complement(4603..5337)
                     /gene="ccoO"
                     /locus_tag="VFL49_05185"
     CDS             complement(4603..5337)
                     /gene="ccoO"
                     /locus_tag="VFL49_05185"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HET7911400.1"
                     /translation="MSLWSRHAIFEKNSIVLVIGILIVIAIGGLVEIVPLFYLKNTIE
                     KVDGVRPYTPLELAGRNIYVREGCYLCHSQMIRPLRDEVERYGHYSLAAESMYDKPFQ
                     WGSKRTGPDLARIGGKYSDEWHRDHLKAPRSVVPGSVMPDYSWLAETNLDFAHIADDL
                     KAQALLGVPYTSDMIANAAADVVTQSTTDSPDAVEMAKRYPKLNARDFDGDPSRISEA
                     DALIAYLQMLGTQVDFKLYDDKANIR"
     gene            complement(5350..6963)
                     /gene="ccoN"
                     /locus_tag="VFL49_05190"
     CDS             complement(5350..6963)
                     /gene="ccoN"
                     /locus_tag="VFL49_05190"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HET7911401.1"
                     /translation="MGEGGSALIFAALAFLSILIAAKAYTPEYAFHAYLFGAASVAAV
                     FAIINRYYERPVDAPPLTIDGKPNYNMGPVKFSAVAAVFWGIAGFTVGLIIALQLAFP
                     VLNFDLPWTTFGRLRPLHTSAVIFAFGGNVLIATSLYVVQRTCRARLAGDIAPWFVVL
                     GYNFFIVIAGTGYLLGITQSKEYAEPEWYADLFLTTVWVVYLLVYLGTIMRRTEPHIY
                     VANWFYLAFIVTIAVLHLGNNAAVPISLFSPKSYIVWSGVQDAMVQWWYGHNAVGFFL
                     TAGFLGLMYYFIPKRCDRPVYSYRLSIIHFWSLIFLYIWAGPHHLHYTALPDWAQTLG
                     MTFSVMLWMPSWGGMINGIMTLSGAWDKLRTDPVLRMMVVSVAFYGMSTFEGPMMSVK
                     IVNSLSHYTDWTIGHVHSGALGWVAYISFGALYCIIPWMWNRPLHSLKLVNWHFWIST
                     IGIVLYVSAMWVSGILQGLMWRAYTNLGFLEYSFVETVEAMHPFYVIRAIGGGLFLAG
                     ALLMAFNLWKTVNATVSAAPADLRLAPAE"
BASE COUNT         1517 a         2112 c         2091 g         1244 t
ORIGIN      
        1 gcccaggcgc gtctcgatgc cgtcgatgtg cgcccgcacg ccctgcccgg gttcctccac
       61 aacctcgtca aatggcacgc gtatcgtggc ctcacgcgcg agcgcaaccg ccagcggatg
      121 ccggctcgac aacgcgagcc gcgccgccat ttgcagcaac tttggatcaa gtgccaccgt
      181 ggtgacgaca cgaggctccg gcagtgtcaa agtgccggtc ttgtcaaaaa taaccgtgtc
      241 aatctccgcc aggcgctcaa tggcgtcgcc ggcgttgagt ataacattcg atcggaatag
      301 cgcgcccgag gtgaccacct gcacggccgg aatcgccaaa gccagcgcac acggacaggt
      361 aataataagc acggcaattg cagtgatgat ggcgtcatgc aggcttgcgc ctgcgatcag
      421 ccagccggct aatgtcaacg cagcaccaag gtgcaccatc ggcgcataga cgcgcgaagc
      481 gcgatcggcg agccgcatgg cgcgcgattt tccgctcgcc gccttttcca agagcttctc
      541 gatctcgtcg atcagaccag cgtcgcccgc agctgtcact ttcagtgtaa gggcgccaga
      601 atagttcatg ctgccggcat agactgttgc tccggatgca acgttacggt gcgccgtttc
      661 gcccgtaatc aagctttcat cgagatcgga ttttccgcca agcaccatgc cgtcggcggg
      721 aacacgctcg cccggtttca ccagcacccg atcgccggct ttcagcgccg ccgatggcac
      781 actgacaagc tcgtcgccga taagccgatg cgcattctcc gctttcagcg ccgcaagatt
      841 gcttgccacc gccctggtct tgcggcgcat ggcatggtcg agagcgcgcc cgaccaacag
      901 gaaaaacagc agcatgatgg cggaatcgaa ataagcgtgc tcggcatggt tggccgtttc
      961 caccagcgac atgccaagag caaggatcac gccgagcgag atcggcacgt ccatattgag
     1021 ggagcgcgcc ttgagcgcgc gccaggcact cgcaaagaat ggccggccgg cataggccgc
     1081 cgcaggcagc gcgatcagcg ccgaggccca atgaaaaaag tcgcgcgttt caggcgtgat
     1141 gtcggcgaca tttcccgacc acaccgacac cgacagcagc atgatgttca tggccgcgaa
     1201 gccggcaacc gccaggcagc gcgtaagacg gcgagcctcg gcagcctcct gctgctcggc
     1261 gcgcatcggc acgaacggat ggccgtggta accgccatct tcgagcgcac gcagaattgc
     1321 ggccggctcc gcgagtcccg gcgaccaaac gacgtgcagc ctacgattcg tgaagttcag
     1381 ccgcgcctcg gtcacgcccg gaacgtttct gaccgcccgc tcgatgcggc cgatgcatcc
     1441 gccgcacgca ataccttcaa cggcaagctc catgccgagc gtgccgtcag ccatcggctt
     1501 ggcataaagc gaaaggtcga ggctctcggt catggcgaat tcaacatcac cctgctgcgc
     1561 gagcggaaaa cgcgcgcatc atccttgtag agatcgacga tcagctccca ttgacccggc
     1621 tcggctcgcg tcacgccgtg aaagcggccg ccggacgcgt cgttgagcgc gatcaaatga
     1681 tcacgccgct catccgccgg atgcgcaaga cgtgcctccg cagcaaggcc cttcacggga
     1741 acgtctctgg tatcccgcac cgatatatca aggacggcat tgccggcgtc atcgcgtgac
     1801 agcctgccgc tgacctgcca atgcagcgcg tcctgctttt ctgcccgagc gaggtcctgc
     1861 ccgaacatca acccggcttt gtaagaactc gtggtctgca cgccgccgaa ggtggacatc
     1921 gccgctttca ccatcacggc attgacggcg aagacgagtc cgaaaaatcc gacaaaacaa
     1981 atcaggacgt gcttaccggt caattcgcga ggcttgcggg aagtgcgcat tttcgctctt
     2041 tcatcatggt ccaaagaaat gatcgcgcat ttcagcggtc tcaccggagt cgatgtcgat
     2101 cagcttgaac agaatcgaag tagatggcgg cggcggcgtg gggctgtagt cggtgacaac
     2161 cacgcgcact tccttggttt gatcggggcc gatcgtgatg agctgccggc cgtcggcgcg
     2221 cggcggcaat ccgacgaaat cgatgagagt cgacggcaat ccgtcaacgc tcatgatgaa
     2281 gtcgcggtac ttcagacgct tgttgacgat gcgaatggta taggcgttgc ggatcgcgcc
     2341 gtcggacagc cgcacgtaca tcggattgcg gtcgtggatg acgctgatgc cctcgctgtc
     2401 gcgcgtggca agcgtgtaga gcatgattcc gccgaccaca gcgatgatgc ccgcgtagag
     2461 agcagtgcgc atgcgcacca gcttgtagaa aggctttttc ccctcgatcc gctgcttgat
     2521 attgagatcg gtgtcgtagg caatcaggcg ggtcggtcgc ccgagcttgc tcatgattgc
     2581 atcgcacgca tcgatgcaca gaccgcactg gatgcagccg agattggcgc ccccgcggat
     2641 atcgaccccc gtcgggcaga catgcacgca ttgcaggcaa tcgatgcagt cgccggccgg
     2701 ctgaccttgc gagcgcagtt gctcggcctt cttgaccgaa caatgcggct caccgcggtc
     2761 atagcggtag gtcacattga gcgcgtattc atcggtgagc gccgcctgaa tgcgcggcca
     2821 cgggcacatg taaagacaga cctgctcacg catgtggccg gcgagcgaat aggtcgtcac
     2881 cgtgagaatg cctatccaga tataggcaat gagcggggct tcccccgtcg caaggtcttt
     2941 caccagagtc ggcgcatccg agaagtaaag aacccacgcg ccgccggtcc accatgccac
     3001 catcagccac aggaaatgct tcgccccttt gcgggcatag gtgccggctg tccacttgcg
     3061 cgcgtcgcgc tgcatgtgct cgcgccgatc gccctcaacg aaccgctcaa tggcaaagaa
     3121 cagatcggtc cacaccgtct gcgggcagag atagccgcac cagatacggc cagcgacggc
     3181 attcatcagg aacagcacta tcgcggcgag aatgagcagc ccggtaaggt agtaaatctc
     3241 ctggggccag atctcgatga agaagaagta aaagcgccgg ttcggaaaat cgatcagcac
     3301 cgcctgggac ggcgcgttcg ggccgcgatc ccagcgcacg aacggaagca ggtaatagat
     3361 accgagcgta acgaccagaa ccgcccattt gatattgcgg aacgtgccgt ggacgctctg
     3421 agggtagatg ttgcggcgtg gcgcatagag cgggacatcg tcttccggcg gcgccccacc
     3481 gacttccgct tccttcacca aatgcgcaag agccggcgca ccgggcgccg gctgatcacg
     3541 cgacatgatg acatgcgcgg tcatttttct ccgccaccga gcgtatgcac gtagaccgtc
     3601 agggccttga tggtcggctc gctcagacgg ccgccccagt ttggcatcac cgcgccgcgt
     3661 ccgttctcaa taccctgaac gatggtcgcc ttgtcggacc cgtataacca gatcttgtcg
     3721 gtcagattgg gagcgcccat ctcgcgatta cctttgccat catcgccatg gcagaccgcg
     3781 caattgtccg cgtagacttt cttgccaagg gcgagatcgg ctttgggttc ggtcgacagg
     3841 ccggagagcg aacgggcaaa atccgctaca gccgagatct cgttgggctt gaggatcttg
     3901 tcgcggccga aagcaggcat gctgccggtg cggcctttgt tgtcactggc ccgcgcgcca
     3961 taccggatcg tttgctcgat atcggcaagc ttgccgcccc agagccattc gtcatcattg
     4021 agattgggat agcccttggc gccgccgcca cccgcgccat ggcacggcgc acaattatcg
     4081 gcaaaggcga cccggccctg cgcccgagca aagtcgagca actgcggatc ggcggcgatc
     4141 tctgcaagcg acgcgccggc gagcttgtcc atcatcgggc cgcgctgctg cttgagcgcg
     4201 tcgagatctt caaaaagcgc ggtccgggtg tgataaccga acaggccctg cgtcgaattg
     4261 gtgaacagtg gccaggccgg gtagacgatc cagtagccaa tcgaccagat aatcgtgatg
     4321 taaaacagcc atagccacca gcgcggcagc ggtgtgttca gttcgcgcag cccgtcccat
     4381 tcatgccccg tggttggggt gccggtcaca gcgtcgattt ctttatgttc ttcgaccacg
     4441 gtcagtcctc ccgcagcgga atgcgcgccg cttcatcaaa tttcttcttg cgcgaaggcc
     4501 agagcgcgta aacgagcacc agcaggaaga cgacgacgaa ataagcgagc ccccaggtct
     4561 gggcgaactc agcgatagca cggtaggttg gatccatggc gatcaccgga tgttggcttt
     4621 gtcgtcgtag agcttgaagt cgacctgcgt tccgagcatc tgcaggtagg caatcagtgc
     4681 atcagcttcc gagatgcgtg acggatcgcc gtcgaaatca cgcgcattca atttcggata
     4741 gcgtttggcc atctccaccg catcggggct gtcggtcgtc gactgcgtga cgacgtcggc
     4801 ggcagcgttg gcgatcatgt ccgacgtata agggacgcca agaagcgcct gcgccttcag
     4861 atcgtccgcg atgtgggcga agtcgagatt ggtctcggcc agccaggaat agtccggcat
     4921 gaccgagccc ggaacaaccg aacgcggtgc tttcagatga tcgcggtgcc attcatccga
     4981 atacttgccg ccgatgcgcg cgagatcggg cccggtgcgc ttcgaccccc actgaaacgg
     5041 cttgtcatac atgctctcgg cggcgagcga ataatggccg tagcgttcga cctcatcgcg
     5101 cagcgggcgg atcatctgcg agtgacagag atagcagccc tcccgtacgt agatgttacg
     5161 tccggcgagt tcgagcggcg tgtagggccg aacgccatcg accttttcga tcgtattctt
     5221 cagatagaac agcggtacga tctcgacgag accgccgatc gcgatgacga tcaggatgcc
     5281 gataaccaga acgatcgagt ttttctcgaa gatcgcgtgt ctagaccaga gtgacatgaa
     5341 tgcccccgct tattccgccg gagccagcct gagatcggcc ggcgccgcgg aaaccgtcgc
     5401 gttcacggtt ttccagagat tgaaagccat cagcaacgcg ccggcgagga acaggccgcc
     5461 tccaatcgcc cggatgacat agaatggatg catcgcttcc acggtctcga cgaacgagta
     5521 ctcgagaaat ccaagattgg tgtaggcgcg ccacatcaat ccctggagaa ttcccgacac
     5581 ccacatcgcg ctgacgtaga gaacgatgcc gatggtcgaa atccagaagt gccagttgac
     5641 gagcttgagc gaatggagcg gccggttcca catccacggg atgatgcagt agagcgcacc
     5701 gaaggatatg tatgcgaccc aaccgagcgc accggaatgc acgtgaccaa tggtccagtc
     5761 tgtgtagtgc gacaatgagt tgacgatctt caccgacatc atcggacctt cgaaggtcga
     5821 catgccgtaa aaagcgacgg aaaccaccat catgcgcagg acgggatcgg tgcgaagttt
     5881 gtcccaggct ccagacagcg tcatgatgcc gttgatcatc ccgccccagg acggcatcca
     5941 cagcatcacc gaaaacgtca taccgagcgt ctgcgcccag tcgggcagtg ccgtgtagtg
     6001 cagatgatgg ggcccggccc agatgtagag aaaaatcagc gaccagaagt ggatgatcga
     6061 aagccgatag gagtagaccg gccggtcgca gcgtttcgga ataaagtagt acatcaatcc
     6121 gaggaagccg gcggtgagga aaaaaccgac ggcgttgtgg ccataccacc actgcaccat
     6181 cgcgtcctga acgcccgacc agacgatgta ggacttcggc gagaaaagag agatcggcac
     6241 cgcagcattg ttgccgagat gcagaaccgc gatcgtgacg atgaaggcga gataaaacca
     6301 gttcgccacg tagatgtgcg gctcggtccg ccgcatgatc gtgccgaggt agaccagaag
     6361 gtagacaacc cacaccgttg tcaggaacag gtcagcatac cattcaggct cggcatattc
     6421 cttggactgc gtaataccga ggagatagcc ggtgccggcg atgacgatga agaagttgta
     6481 gccaagcacg acgaaccacg gcgcaatgtc gcccgcgagc cgcgcgcggc aggtccgctg
     6541 cacgacgtag agcgacgttg cgatgagcac gttgccaccg aatgcgaaga tgaccgccga
     6601 tgtatgcaac gggcgcaacc ggccgaaggt cgtccagggc agatcgaagt tgaggacggg
     6661 gaacgcgagc tgcagtgcga tgatcagtcc gactgtaaat cccgcgatgc cccagaacac
     6721 tgccgcaacc gcagagaact tgacgggacc catattgtaa tttggtttgc cgtcgatggt
     6781 cagcggcgga gcatcaaccg ggcgttcata gtagcgattg atgatggcaa ataccgctgc
     6841 gacgctggcg gcaccgaaaa ggtaggcatg gaacgcgtat tccggcgtgt acgctttagc
     6901 cgcgatgagg atcgataaaa atgcgagggc tgcaaatata agcgctgacc cgccctcccc
     6961 gatc
//
LOCUS       DASSRL010000252         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_636747, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000252 DASSRL010000000
VERSION     DASSRL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636747"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFL49_05195"
     CDS             complement(<1..952)
                     /locus_tag="VFL49_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HET7911402.1"
                     /translation="MLTVYNCLVDEHDLRLVVLAALVCAIASFAALSLLHHVRRSTGY
                     MRAVWLTVSAISTGFGIWATHFVAMLAYSPAIQNAYSVPLTSLSLVAAIVLTGVGLAV
                     AVSSKFRGGTWIGGGLVGGGIAVMHYTGMAAFEVQGRIIWDLPLVTASIVLGAVIGAA
                     ALPVGLRADSLKWKLSGALLLTLAICSHHFTAMGAASLIPDPTISISDTALPSGWLAI
                     AVAVASAIILMLALAGVALEMRDRRRGELENARMRGLANAAIEGLVVCEGDKIITVND
                     SFSALAGRPAGAIVGTNLEQHFAEEVIRLRLWDRPNNAVEG"
     gene            complement(1112..>2141)
                     /locus_tag="VFL49_05200"
     CDS             complement(1112..>2141)
                     /locus_tag="VFL49_05200"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7911403.1"
                     /translation="FEQALKAADYDGFRERKKAAKKRGKLRGVGIGCYLEISGAFPEE
                     AARVSFPGGKSVQVSVGAGASGQGHKSVFGRVAARRLGIRPDAITVTSGDSTRDVPGF
                     GAVASRSAMMTGGAVARTADAVLEKGKRVAAMLLQADENEIEFGDGKFRVKNSAREVS
                     LFEAAERAAELKRQGVIPESLDTQAGIKVPPSFPNGCHIAEVEIDPSTGAVDVVSYVA
                     IDDCGNLLDHIIVEAQIHGGVAQGLGQALMENTVYDAGGQLMSGSFMDYAMPRADMMP
                     GMKVEHHAVACRTNPLGVKGTGEAGTTAAPPAVMNAILDALPAGAQIEMPATSERVWL
                     ALRATSST"
BASE COUNT          424 a          701 c          645 g          371 t
ORIGIN      
        1 caccctcgac ggcattattg gggcggtccc ataacctcag gcgtatcact tcctcggcga
       61 aatgctgttc gaggttcgtg ccgacgattg ctcccgctgg acggccggca agagccgaga
      121 agctgtcgtt gacggtgatg atcttgtccc cttcgcagac gacgagacct tcgatcgcgg
      181 cattggcaag accgcgcatc cgcgcattct caagttcgcc acgccggcgg tcgcgcattt
      241 ccaatgcaac gcccgcgagc gcaagcatca ggatgatggc gctggcaaca gctaccgcaa
      301 tcgcaagcca gcctgaaggc agggccgtat cggagatcga aattgtcggg tccggaatga
      361 gcgaggcggc gcccatcgcg gtgaagtgat ggctgcaaat cgccaaggtc agcagcagcg
      421 cgcccgacag tttccatttc agagaatcag cgcgcagccc gacgggaagt gcagccgcac
      481 cgatcaccgc ccccaacaca atcgacgcag tgacaagcgg cagatcccaa atgatccttc
      541 cctgaacttc gaacgccgcc atgccggtgt aatgcatcac cgcaatgccg ccgccgacca
      601 gcccgccacc tatccaggtt cccccgcgga atttcgatga gaccgccacg gccagaccga
      661 cgccggtcag gacgatagcg gcgacgagcg agagagaggt caacggcacg ctgtaggcat
      721 tctgaattgc cggcgagtac gccagcatcg caacaaagtg cgtcgcccag attccgaatc
      781 cggttgaaat cgctgaaaca gtcagccaga cagcccgcat gtagccggtc gatctgcgaa
      841 cgtgatgaag caaggacaac gccgcaaacg aggcaatggc gcagacgagc gcggcgagta
      901 cgacaagccg gaggtcgtgc tcatcaacga ggcaattgta gaccgtcagc atcccgtcac
      961 cgcacctgtg ttggcttcgc agggaaggtc gcgcacgcta cattttcgtg ctgcactaaa
     1021 agcttaatgc cggcgcgcaa gaatggcgca cgcggtgcag cggctgcact tttggttagg
     1081 gggttgttaa cgcaacgcga ggcggaggcg atcaggtgga actggttgcc cgcaaggcca
     1141 gccatacacg ctccgatgtc gccggcattt cgatctgcgc ccccgccgga agcgcatcga
     1201 gtatggcgtt catcaccgcc ggcggcgccg ctgtcgtgcc ggcctctccg gttcccttca
     1261 cgccgagcgg attggtgcgg caggcgacag cgtggtgctc caccttcatg cccggcatca
     1321 tgtcggcgcg cggcatcgcg tagtccatga acgagccgga catgagctgg cctccggcgt
     1381 cgtagacggt gttttccatg agcgcctggc caaggccctg tgcgactccg ccgtgaattt
     1441 gcgcctcgac gataatgtga tcaagcaggt tgccgcagtc gtcgatagcg acataagaca
     1501 cgacgtcgac ggcgcccgtt gatggatcaa tctcgacttc cgcgatatga cagccattgg
     1561 gaaacgacgg cggcaccttg atgccggcct gcgtgtcgag gctctccggg atgacaccct
     1621 ggcgtttgag ctcggcggcg cgctccgccg cttcgaacag cgagacctca cgcgcggaat
     1681 ttttcaccct gaacttgccg tccccgaatt cgatctcatt ttcgtccgcc tgcaacagca
     1741 tggcagcgac acgcttgcct ttctcaagga ccgcatcggc cgtgcgcgca acggcgccgc
     1801 ccgtcatcat ggccgagcgc gacgcaaccg cgccgaagcc cgggacgtcg cgggtggaat
     1861 cgcccgacgt cacggtgatc gcgtccggcc gtatccccag gcgccgggcg gcgacgcggc
     1921 caaacacgga cttgtgcccc tggccgctcg cgcctgcccc gacgctgacc tgcacggatt
     1981 ttccgccggg aaatgaaaca cgcgccgctt cttccggaaa ggctccggat atttccaggt
     2041 agcagccaat gccgacgccg cgcagcttgc cgcgcttctt cgcggctttt tttcgctcac
     2101 ggaagccatc ataatcagcc gccttgagcg cttgctcgaa c
//
LOCUS       DASSRL010000253         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1458735, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000253 DASSRL010000000
VERSION     DASSRL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458735"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..2101)
                     /locus_tag="VFL49_05205"
     CDS             complement(428..2101)
                     /locus_tag="VFL49_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7911404.1"
                     /translation="MDNPDFLIIGGGSAGAVIASRLSEDPQTRVLLVEAGRDTPPDAL
                     PADIADTFPASSLNPDYFWPGLQAVRTSGGPARPFPQARIMGGGSSVMGLWALRGVPS
                     DFDAWIAAGAEGWSWADVLPYYRKLEDDFDRDQSQTSRGTYPIRRLPRDEWPAFVTAI
                     EGAAKARGLPLVEDINERPGPGFFPMPLSQDMTTRASSARTYLTTAVRRRPNLKILTE
                     AQVTALRFDGLRTCGATIAHGSSISDISAREVILCAGAIHSPAMLLRAGVGPPDELRK
                     IGITPRLDRPGVGRNLQNHPYLHFALTLPRRSRLQNDLRRFAIAGARISSGQEEAPPA
                     DLLLFTIGRVSSRDYGTDVAMVGCALYAPYSHGAVSLASADAKALPRIDFRLLEDPRD
                     PPRMLIAARFAESMLCSPGVAATYNDAFLLPPVMSLHQFNRPGATGDIFAFVAKTVLN
                     APAAVSRQVIGRVIRPGKWFANKRRRRELSDEQLLAAAAPMAHPVGTCSIGRPDNPMA
                     VVDRWCRVYGAYSLRVADASIMPRIPSANTNLPTIMIAERISDLIRHGR"
BASE COUNT          444 a          648 c          711 g          448 t
ORIGIN      
        1 ttggcgccgc cggttgcgcg gttgccgggt cccgacgaac agggctcctg agggaaaggc
       61 tttgggcgcc gctgcatcta ggcgcgtcgc gcccgggcct ttttcctcgg ttccgtgagg
      121 ggtagccgaa gctgtcttcg cttccgtttc tgctgcagcg acgccggccg ctcccggcgt
      181 ccgcatagtg cgctggtctg atgctggtac gatcccggca aggccagggg tcagtcagaa
      241 ggaaatttcg aggaaagtgg ctactcgact caaaatgact cacatgactc actattgggc
      301 ggtcggcgtt cacgtgacag ccgataagca gcgattatcg gtggtctggc tttaagaaaa
      361 atctcacaca cgatcgggtg gttgcaagcg gttatagccg caaagagatg ctggcttttg
      421 gcaatgctca ccggccgtgg cggatcaagt cgctaatacg ttcggcgatc atgatcgtcg
      481 ggagattggt gttggcgctt ggaatgcgtg gcattatcga ggcatcggca acgcgcaggc
      541 tgtaggcgcc gtaaacccga caccaacggt caacgaccgc cattggatta tccggtcggc
      601 ctattgaaca tgtgccaacc ggatgtgcca ttggcgccgc ggcagcaagc agttgttcgt
      661 cacttaattc gcgtcgcctt cgtttattgg cgaaccactt gcctggacgg ataactctgc
      721 caataacctg gcgactaacg gcggccggcg cattcaacac ggtcttggcg acgaaagcga
      781 aaatgtcacc tgttgctcct ggccggttga actggtgcaa agacatgacc ggcggcaata
      841 aaaacgcgtc attatacgtg gcagccaccc cgggagagca cagcatgctt tcggcaaagc
      901 gagccgcgat gagcatccgc ggcgggtcgc gcggatcctc tagcagccgg aaatcaatgc
      961 gcggtaatgc cttcgcgtcc gcactcgcta gcgagacggc gccatgggaa tagggcgcgt
     1021 aaagtgcgca tcccaccatt gctacgtctg taccatagtc gcgcgagctg acgcgtccga
     1081 tcgtgaacag cagcagatca gcgggaggcg cttcctcctg acccgacgag atgcgagcac
     1141 cggcaatggc aaatctgcgc aaatcgtttt gtagccgtga acgacgcgga agggtcaggg
     1201 cgaaatgcaa atacggatgg ttttgcaggt tgcggccgac gccgggccgg tcgagtcgag
     1261 gcgtaatgcc gattttgcgc agttcgtccg ggggaccgac gcccgcacgc agtagcatgg
     1321 ccggtgaatg gatcgcaccg gcgcacagaa tgacttcgcg cgcactgatg tcgctgatgc
     1381 tgctgccatg cgcgatggtc gcgccgcagg tcctcaggcc gtcaaagcgg agtgcggtca
     1441 cttgcgcttc cgtcaggatc ttgaggttcg gtcggcggcg aacggcggtg gtgaggtagg
     1501 tgcgggcact tgatgcgcgc gtcgtcatgt cttggctcag cggcatcgga aaaaagcccg
     1561 gccctgggcg ctcgttgatg tcttcgacca gcggcaggcc ccgcgccttg gccgctccct
     1621 cgatggcggt gacgaaagca ggccactcgt ctcgcggcag acgtcgaatg gggtaagtgc
     1681 cgcgtgaggt ttggctttga tcacggtcaa agtcgtcttc cagcttgcgg taataaggaa
     1741 gtacgtccgc ccagctccag ccttcggcgc cagcggcgat ccatgcatca aaatcagacg
     1801 gtacgccccg caaagcccac aggcccatca cactcgatcc gccgcccatt atgcgtgcct
     1861 gtggaaacgg gcgcgccgga ccgcctgacg ttcggaccgc ttgcaggccg ggccagaaat
     1921 aatccggatt caacgatgaa gccggaaagg tatcggcgat atcggcgggc agtgcatccg
     1981 ggggtgtatc acggccggct tcgaccagca gcacgcgcgt ttgaggatct tcagaaagcc
     2041 gcgatgcaat gaccgcgcca gcgctgccgc cgccgataat tagaaagtca ggattgtcca
     2101 tgagccaatc gaggtgagta tgcacggcga gttaaccaac cagcttgcgt ccgttcgatc
     2161 aagcctcgcc cttcttgccc atgattattt cgtgccctta ggccgtttgc gatcgtttct
     2221 ctgatgcggt gtagcagatt tcggcttcat c
//
LOCUS       DASSRL010000254         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1633896, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000254 DASSRL010000000
VERSION     DASSRL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633896"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFL49_05210"
     CDS             <1..201
                     /locus_tag="VFL49_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET7911405.1"
                     /translation="IQTLVRHLTNRGIGVLITDHNVRETLGLTDRAYIIYSGEVLMEG
                     RADDIVNNPDVRRLYLGEEFRL"
     gene            326..1843
                     /gene="rpoN"
                     /locus_tag="VFL49_05215"
     CDS             326..1843
                     /gene="rpoN"
                     /locus_tag="VFL49_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET7911406.1"
                     /translation="MVLSQRLEIRQSQALVMTPQLMQAIKLLQLSSLDLAAYVEGELE
                     RNPLLERAGDDEIGAGGEPQASSGSDDIAGGADWINADLETSRESIEQGLGTELENVF
                     PDDGADKSTPARELRPEAYSEWSGTGPAGGEDYNLEAFVSAETTLADHLAEQMTLAIS
                     SPAGRIIGRYLIDMVDEAGYLAGDLDTVAEKLGAQRSDVELVLAVLQTFDPLGVCARN
                     LAECLAIQLKDRNRYDPAMQTLVEHLDLVARRDLAGLRRLCGVTDEDLADMIAEVRML
                     KPKPGLTFGSTMVQPIVPDVFVRAAPDGTWQVELNSDTLPKVLINQRYYAQVSGSTRN
                     EKEKTYLADCLQTATWLVRALDQRAKTILKVSSEIVRQQDGFFARGVQFLRPLNLKAV
                     ADAIGMHESTVSRVTANKYMATSRGIFELKYFFTSAIAAADGGEAHSAEAVRHRIRQL
                     IDDESSENVLSDDTIVDKLRNAGIDIARRTVAKYREAMRIPSSVQRRREKQASGA"
     gene            1945..2256
                     /gene="raiA"
                     /locus_tag="VFL49_05220"
     CDS             1945..2256
                     /gene="raiA"
                     /locus_tag="VFL49_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7911407.1"
                     /translation="MPLRVSGRNISIGAALQRKITDRVEEATSKYFRGGYSGHATVGR
                     DGFGFRTECILHLDSGALLEAEGMAPNAYDSAEQAALRIEKRLRRYKRRRKDHQSRRG
                     G"
     gene            2387..2851
                     /gene="ptsN"
                     /locus_tag="VFL49_05225"
     CDS             2387..2851
                     /gene="ptsN"
                     /locus_tag="VFL49_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HET7911408.1"
                     /translation="MTLTDFVAPGAVIAALKVTSKKQAIQELAARAAELTGQSEKEIL
                     EILLQREKLGSTGVGSGVAIPHGKLPKLGRLFGLFARLQRPIDFEALDNQPVDLIFLL
                     LAPEAAGADHLKALARVARQLRDPEIARRLRESTDADALYAVLAMPPASTAA"
     gene            complement(2948..3223)
                     /locus_tag="VFL49_05230"
     CDS             complement(2948..3223)
                     /locus_tag="VFL49_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HET7911409.1"
                     /translation="MTNDKQIEPNIAHPVITQEALAQLGDGRLAYVKTIRSEDVANLF
                     PQAPKIAPGIKLFALHAADGTPIMLTDTREAAVANAWSQELEPVSVH"
     gene            complement(3284..3709)
                     /locus_tag="VFL49_05235"
     CDS             complement(3284..3709)
                     /locus_tag="VFL49_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HET7911410.1"
                     /translation="MSRVPSLSSPFLLGFDEIERVLDRVAKGADGYPPYNIERLQRDG
                     QNPERLRITLAVAGFTRDQLDVSIEESQLVIRGRQQDDKTRQYLHRGIAARQFQRTFV
                     LCDGMEVLGADLKNGLLSIDLIRPQPERIVKTISINEQE"
     gene            3882..>4497
                     /locus_tag="VFL49_05240"
     CDS             3882..>4497
                     /locus_tag="VFL49_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7911411.1"
                     /translation="MPIDPEFTPLTIRSLRGTPVEVPLNFVLGTSAVAVRRAPLLLVD
                     LDTEEGVTGRTYLFCYLPAAAPAIISLLEEVERIVKGETIAPAELWKKLARRFTLIGV
                     QGIVRMAMSAFDAAAWDALAIAAGKPLAAFIGATPRNIPAYNSCGLGLNDDLGALADE
                     AEKLLAGGFRAIKLRLGYPTVQEDISAVHAVRKRIGDDVALMVDY"
BASE COUNT          926 a         1314 c         1363 g          894 t
ORIGIN      
        1 attcagacac tagtgcggca cctgaccaat cgcgggattg gcgtcctgat caccgaccac
       61 aatgtgcgtg agacactggg actcaccgat cgggcctaca tcatctattc cggagaggtg
      121 ctgatggagg gccgcgccga cgatatcgtg aacaatcccg acgtgcggcg gctttatctc
      181 ggcgaggaat tccggctcta gcagcgctga aaagttaaca cgcacaagaa ggaggcaggc
      241 tctgcctgct aaatcacgta ccgcgactcg caggcagctg gtataaacaa gcaagaatcg
      301 gaccagtttc cgccgggatt tctgaatggt tttgtcgcaa aggctggaga tccgccagtc
      361 tcaagcgctg gtgatgacgc cgcagttgat gcaggccatc aagctgttgc aattgtccag
      421 cctcgacctc gcggcctatg tggaaggtga actggagcgc aatccgttgc tcgagcgcgc
      481 cggtgacgac gagattggcg caggtggcga gccgcaagct tcatcaggaa gtgacgacat
      541 cgccggaggc gccgactgga tcaacgccga ccttgagacg agccgggagt ccatcgaaca
      601 ggggctcggc accgaactcg aaaacgtgtt tcccgacgat ggcgcggaca agagcacgcc
      661 cgcgcgtgaa ttgcggccgg aagcctattc ggagtggtcg ggtacgggac cggcaggcgg
      721 cgaagattac aatcttgaag cattcgtctc ggccgaaacg acgctcgccg accatctcgc
      781 tgagcagatg acgctggcca tttccagtcc ggccggccgc atcatcggcc ggtacctgat
      841 cgacatggtc gatgaagcag gctacctggc tggcgatctc gacacggtcg ccgagaagct
      901 cggtgcgcag cgcagtgacg tcgagcttgt gcttgcggtt ctgcagacgt tcgatccctt
      961 gggagtctgc gcgcgcaatc tggccgaatg cctcgcgatc cagctcaagg accgcaaccg
     1021 ttacgatccg gcaatgcaga ccttggtgga gcatctggat ctcgtcgcca ggcgcgatct
     1081 tgccgggttg cgccggctgt gcggcgttac cgacgaggat ctcgccgaca tgatcgccga
     1141 ggttcgcatg ctcaagccaa agccaggttt gacattcggc tcgacgatgg tgcaaccgat
     1201 cgttccggac gtgttcgtgc gtgcggcgcc cgacggcacc tggcaggttg aactcaattc
     1261 cgacacgctt ccgaaagtcc tcatcaatca gcgctattac gcgcaggttt cgggcagcac
     1321 gcgcaatgaa aaagaaaaaa cctacctcgc ggactgtctg cagacggcca cctggctggt
     1381 gcgcgcgctt gatcagcggg caaagacgat tctgaaggtg tcgagtgaaa tcgtgcgcca
     1441 gcaggacggg tttttcgcaa gaggcgtgca attcttgcgg ccgctcaatc tcaaggctgt
     1501 tgccgatgcc atcggaatgc acgagtcgac ggtttcgcgg gtgaccgcca acaaatacat
     1561 ggcgacgagc cgcggcattt ttgaattgaa atattttttc acctccgcaa tcgccgcagc
     1621 cgacggcggt gaagcccatt cggccgaggc ggttcggcat cgcatccgcc agctgatcga
     1681 cgatgagagt tcggaaaacg tgttatcgga cgacacgatt gtggacaaac tgcgcaatgc
     1741 tggaatcgac atcgcgcggc gcacggttgc gaagtaccgt gaggcaatgc gtattccatc
     1801 gtcggtgcaa cgccggcgcg aaaagcaggc cagcggcgcc taaaaaaacc cccgtctcaa
     1861 ggcattgacg cgcggaacaa atctacgctg aaatctcaat gtctgcccaa aaggcggcgt
     1921 cacgagtccc aatggaggca aacaatgcct ttgcgcgtct cgggtcgaaa catcagcatc
     1981 ggcgcggcat tgcagcgaaa aatcaccgac cgcgtcgaag aggcaacctc aaaatatttt
     2041 cgcggcgggt attcggggca tgcaacggtc ggcagagacg gcttcgggtt ccgtactgaa
     2101 tgtatcctgc atctcgattc gggcgcgttg ctggaagctg aaggaatggc gccgaacgcc
     2161 tacgacagtg ccgagcaggc agctctccga atcgaaaaaa ggctgcgacg ctacaaacgc
     2221 cgccgcaaag atcatcaatc gcgtcgtggc ggctaacatc gccaaaaatg gcactatatt
     2281 gcgcgagtaa tgccattgac ggcgggccgt acggcactta tggtccgccg ccttcaacac
     2341 cccctttgca actcggcgct gtcattctcg atcattggcc acgtccatga cgcttaccga
     2401 tttcgtcgcg cccggcgccg tcattgccgc gctcaaggtt accagcaaga aacaggcgat
     2461 tcaggaactc gctgcgcgtg cggcagaact gacagggcaa agcgagaaag aaattctgga
     2521 aatactgctt cagcgcgaaa agctcggctc gaccggcgtc ggtagcggcg tggcgattcc
     2581 gcatggcaaa ttgccaaaac tcggccggct ctttggtctt tttgcacgac tgcaacggcc
     2641 gatcgatttc gaggcgctcg acaaccagcc ggtcgatctc attttcctgc tgctggcgcc
     2701 agaagcggcc ggcgccgatc atctcaaggc attggcgcgc gttgcgcgcc agctgcgcga
     2761 tcccgagatc gcgcgccgcc tgcgcgaatc aacagacgct gacgcgctct atgcggtgct
     2821 tgcgatgccg ccggcgagca cggcggcctg atcagggatc agtcgtcagt aatcaaaaga
     2881 gcgcggaacg gcactgtccc gcgctctttg gcgatctgat tcctgatatc tgatgactga
     2941 tccctgatca gtgcacgctc acgggctcga gctcctggct ccaggcgttc gccacggcgg
     3001 cttcacgcgt gtctgtcagc atgatcggcg tgccgtcggc cgcatgcaga gcgaacagtt
     3061 tgatgcctgg cgcgattttt ggcgcctgcg ggaacagatt ggcgacatcc tcggaacgaa
     3121 tcgtcttcac gtaagcgaga cgaccgtcac cgagttgtgc cagcgcctcc tgcgttataa
     3181 ccgggtgcgc gatgttcggc tcaatctgtt tgtcgttggt catggccctc gactcctttc
     3241 tgattcaacg cttgacgcgg tcgagtcctg cttcacgagt ccgtcattcc tgctcgttga
     3301 tcgagatcgt tttcacgatc cgttccggtt gtggccggat cagatcgatc gacaacaggc
     3361 cgtttttcag gtcggcgccc agaacctcca tgccatcgca gagcacaaac gtgcgctgaa
     3421 actggcgtgc ggcgatgccg cgatggagat attggcgggt cttgtcgtcc tgctgccgcc
     3481 cgcggatcac caattggctt tcctcgatgc tgacatcgag ttgatcgcgg gtgaacccgg
     3541 caaccgcgag cgtaatacgc agccgttccg ggttttgccc gtcgcgctgc agccgctcga
     3601 tgttgtacgg cgggtagccg tcagctcctt tcgcaacgcg gtcgagcaca cgctcgatct
     3661 cgtcgaatcc gaggaggaac gggctggaga gagacggaac tcgtgacatt ggtctcaaag
     3721 ccctcgcttg aagcgacttt gacggggccc ctttctgagc accccaaccg aaaagcgact
     3781 gctctccggt cgttcctaat atgggcaagc cgggttaaag gttcaagaac gggcggaatc
     3841 cggtccggac aggaaatatt gccagctttc aaaggcttct catgcctatt gaccccgaat
     3901 ttacgccgtt gaccatccgc agcctgcgcg gcaccccggt ggaagttccg ttgaacttcg
     3961 tgctcggtac cagcgcggtc gccgtgcgca gggcgccgct tctgctggtg gatctggaca
     4021 ctgaggaagg ggtaaccggc cgcacatatc tgttctgcta cctgcctgcg gctgcgccgg
     4081 cgatcatcag tctgctcgag gaggtcgagc ggatcgtgaa aggtgagacc attgcaccgg
     4141 ctgagctttg gaagaaactc gcacgccgat ttacgttgat cggcgtgcag ggcatcgtcc
     4201 gcatggcaat gtcggctttt gatgcggccg cttgggacgc acttgccata gcggccggca
     4261 agccgcttgc ggcattcata ggggcgacgc cgcgcaatat ccccgcatac aacagctgtg
     4321 gtctcggatt gaacgacgat ctgggcgcac tcgccgatga agctgagaaa ttgcttgccg
     4381 gaggctttcg cgcaatcaag cttcgattgg gttacccgac ggtgcaagag gatatttcgg
     4441 cggttcacgc cgtgcgcaag cgcatcggcg acgatgtggc tttaatggtt gactaca
//
LOCUS       DASSRL010000255         7741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1161643, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000255 DASSRL010000000
VERSION     DASSRL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161643"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFL49_05245"
     CDS             <1..790
                     /locus_tag="VFL49_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HET7911412.1"
                     /translation="GAAIILVGHVTKDGQIAGPRVVEHMVDAVLSFEGEGSHQFRILR
                     AVKNRFGPTDEIGVFEMTGAGLREVANPSELFLSERDLGQPGTAVFAGIEGTRPLLVE
                     IQALVAPTSLGTPRRAVVGWDPSRLSMVLAVLEAHCGVRLGGHDVYLNVAGGMRIQEP
                     AGDLAAAAALVSSLANAPLPADAVYFGEVSLSGAVRPVAQSASRLKEAAKLGFNRAIV
                     PEAARAETEPGFKVSDVRSLAALVADIAVRGKAPRLVKTGGQDP"
     gene            1011..1607
                     /locus_tag="VFL49_05250"
     CDS             1011..1607
                     /locus_tag="VFL49_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7911413.1"
                     /translation="MPITLLDIILLAVMLVSGLLAMIRGFMREILSIGAWGIAALVTL
                     YSYSRLLPTAKQYFSSDMVAAGVTAGGVFLGTLLVVSIITARISDMVLDSRVGALDRT
                     LGFLFGLGRGLIIVVVAFLFFAWLVPDRSQPDWVRSAKSKVVLQNTGQWLMSMLPDDP
                     ESTILKRLKRPKPEDQDAPDTNPDQRSELLTQPRASVR"
     gene            1640..3100
                     /gene="purF"
                     /locus_tag="VFL49_05255"
     CDS             1640..3100
                     /gene="purF"
                     /locus_tag="VFL49_05255"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7911414.1"
                     /translation="MTDEAFDPDADRLREECGVFGIFGHPDAAAITALGLHALQHRGQ
                     EAAGIVSFDGKRFHSERRLGLVGDAFARREVIDRLPGTSAIGHVRYSTTGETIIRNVQ
                     PLFAELNAGGFAIGHNGNLTNGIKLRRDLVHEGAMMQSTTDTEVILHLVARAKRNRFV
                     DRFIEGLRQLEGAYAFVGLTNKKLVGARDPLGIRPLVVGRLDGCPILASETCALDIIG
                     AQYVRDVENGEVVIFDEDGAQSHKPFPPMPERPCIFEYVYFSRPDSIVGGRHVYNVRK
                     TMGAQLAREAPAPADVIIPVPDSGVPAAIGFAQEAKIPYELGIIRNHYVGRTFIQPTQ
                     AIRDQGVRLKHSANRAVVAGKRIVLVDDSIVRGTTSRKIVQMMRDAGAREVHFRIASP
                     PITHPDYYGIDTPERDKLLAATHDLEGMRKFIGADSLAFLSVDGIYRAMGFEGRDPVR
                     PQFTDHCFTGDYPTPLTDLAAQEASPRQLSLLAEAI"
     gene            3121..3870
                     /locus_tag="VFL49_05260"
     CDS             3121..3870
                     /locus_tag="VFL49_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7911415.1"
                     /translation="MTGTTGPLSDRIALVTGASRGIGAAVALELAKAGAHIVAVARTV
                     GGLEELDDKIKAAGSSATLVPLDMKDSDGIARLALALNDRYQRLDVLIGNAGVLGTLS
                     PLSHYEPKDWDNMIAVNVTANWQLIRCMDALLQRSQAGRAVFLTSGVAHLDRAYWGPY
                     AASKAALEVLVRTYAAETATTNVRANLFGPGPTRTRMYLGAFPGVDPLTLPTPEEVAK
                     AILPLCLPGFTESGKLYDYRDRKLKAFQAPA"
     gene            complement(3875..5236)
                     /gene="der"
                     /locus_tag="VFL49_05265"
     CDS             complement(3875..5236)
                     /gene="der"
                     /locus_tag="VFL49_05265"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7911416.1"
                     /translation="MPFTVAIIGRPNVGKSTLFNRLVGRRLALVDDRPGVTRDRREGE
                     ARLGDIAFRVIDTAGLEQSKPESLSGRMQAQTDAAIDQADAVFFMIDARTGPMPADHA
                     FADVARKSGKPVVLVANKSEGTAGEAGRVEAYALGLGDPVPISAEHDEGMSDLYDALR
                     AVLPEQSGADSSGLAMDEAHPIRVAIVGRPNAGKSTLINRLIGEDRLLTGPEAGITRD
                     AIAVEHVWQHHKFRIYDTAGLRRRSRVDEKLEKLSVADALNAVRFAEVVVVLLDVENA
                     FEEQDQRIADLVEQEGRAIVIAVNKWDLNEKKAGAIGKLREQAEEKLTQLKGVPLVAV
                     SGLTGEGLDRLMQAVVDAYDVWNKRIPTSGLNRWFEHAVSAHPPPAVSGRALRLNYIT
                     QAKSRPPSFVIFCTRADAVPDAYKRYLLNSLRETFELPGTPIRLTLREKDNPYKSRAK
                     RKH"
     gene            complement(5290..5829)
                     /locus_tag="VFL49_05270"
     CDS             complement(5290..5829)
                     /locus_tag="VFL49_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911417.1"
                     /translation="MERAERMRAISRIVVLAVLAAAVPALAGCSNFDPDKLDVFGLNE
                     KKKLPGERKELFPGGVPGVTQGIPPEYMKGNQPPPETAQALQAPATEQNKAEAEPEKK
                     AAAAEPEPKPKPKRAAKPRTAPQQSTQAARQPQQQMEPWPQQNPQPAPQQAQSPWPAP
                     TQAAPAPWPSSPQPGTFSR"
     gene            complement(5816..6469)
                     /locus_tag="VFL49_05275"
     CDS             complement(5816..6469)
                     /locus_tag="VFL49_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7911418.1"
                     /translation="MADIFHEVDEEVRREQLKKLWDRYSIHLIALAVLIVAGIAGWRG
                     YEYWVAKKAAVAGAQFEAAIALSEQGKEAEAEAAFAKVAAEAPAGYRVLARMRAASVL
                     AQSKPADAVKAFDELSADASLGAIWQDLAAVRAGMLLVDTAPLADMRRRLDPVAEPTR
                     SFRHTARELLALSAWRNHDFAAARKYLDMIAGDAESPIGVRARADVLSALITADGKS"
     gene            complement(6581..7408)
                     /gene="panB"
                     /locus_tag="VFL49_05280"
     CDS             complement(6581..7408)
                     /gene="panB"
                     /locus_tag="VFL49_05280"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7911419.1"
                     /translation="MSIQDEIRRFTAPDILARKGAEPIVCLTSYHAHTARLVDKYCDV
                     ILVGDSLGMVMHGMETTVPVTLDMMILQGHAVMRGSRRALVVVDMPFGSYEESKEQAF
                     MNAARVLKETGCGAIKVEGGKRMAETIRFLAERGVPVMAHVGLTPQAINAIGSFRAQG
                     RDEADWARIEEDTVAVAQAGAFSVVIEAVAEPLARKLTAAVAIPTIGIGASVACDGQI
                     LVLEDMLGLSPRVPKFVKRYGSLGPGIEKAISDYAAEVRARSFPGPEHVYPMKSKKV"
     gene            complement(7412..>7741)
                     /locus_tag="VFL49_05285"
     CDS             complement(7412..>7741)
                     /locus_tag="VFL49_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HET7911420.1"
                     /translation="VIFLVSAFIPCRIRKWLKHPMLTSVKTWALAHLLSNGDLGGIIL
                     FGSFLLWGGYSRAAAKRRGDLAAAAKPVPEGWANDIIVVVLGAAIFLALGYWFHPYVI
                     GVSVFGR"
BASE COUNT         1402 a         2513 c         2353 g         1473 t
ORIGIN      
        1 cggcgcggcg atcattctgg tcggtcatgt caccaaggat gggcagatcg ccggtccgcg
       61 cgtcgtcgag cacatggtcg atgcggtgct ctcattcgag ggcgaaggct cgcaccaatt
      121 ccgtatcctg cgcgcggtca agaaccggtt cggaccgacc gatgaaatag gcgtttttga
      181 aatgaccggt gcgggtttgc gcgaggtcgc caatccgtcc gaattgtttc tgtccgagcg
      241 cgatctcggc caacccggta cggctgtctt cgcagggatc gagggcacac ggccgttgct
      301 ggtcgaaatc caggcgctcg ttgcgcccac atcgcttggt accccacggc gcgccgtcgt
      361 cggttgggac cccagccgtc tctcgatggt gctggcggtg ctggaagccc actgcggggt
      421 gcggctgggc ggacacgatg tctaccttaa tgtggccggc ggtatgcgta ttcaggagcc
      481 ggccggcgac ctagcagcag ccgcagcact ggtctcgtcg ctcgccaatg ctccgctacc
      541 ggcggatgcg gtctattttg gggaagtatc gctctccggg gcagtgcgcc cggtggcgca
      601 gagcgcctcc cggctgaagg aagcggcaaa gctcggtttc aatcgcgcca ttgtcccgga
      661 ggctgcgcgc gccgagaccg agcccggctt taaggttagt gatgtacgaa gccttgcagc
      721 gctcgtcgct gacatcgccg ttcgtggtaa agcgccgcgg ctggtcaaaa ctggtggaca
      781 agacccgtga cggcgccccg gcgccccgct atacacgggc gtcaaaaacc ggctccggcg
      841 gcctctacgg gcaccccgcc gccagcgtaa aaatgggcta aaaattaagc acttaaggtc
      901 gagattgccg cccctgtccg gagccgctat ggctggcccg gattcgtcgc gctggcgccg
      961 tgccagacgc tacggcaaac gactgagttc gcggagcgcc tgagcacccg atgccgatca
     1021 cgctgctcga catcattctg ctggcggtca tgttggtctc aggcctgctt gccatgatcc
     1081 gcggcttcat gcgcgaaatt ctctcgatcg gagcctgggg cattgcggcc ctggtgacac
     1141 tgtattcgta ttcacggctg ctgccgacag cgaagcaata tttcagcagc gacatggtcg
     1201 cggccggagt gacagccggt ggcgttttcc ttggcacact tctggtcgtc tcgattatca
     1261 ccgcgcgcat ttccgacatg gtccttgaca gccgggtcgg tgcactcgac cgcacgctcg
     1321 gtttcctttt cggcctcggc cgtgggctta tcatcgtcgt ggtggctttc ctcttctttg
     1381 cgtggctcgt tcccgaccgc agccagcccg attgggtgcg cagcgccaag tcgaaagtcg
     1441 tgctgcaaaa taccgggcaa tggctgatgt cgatgttgcc ggacgacccc gagagcacca
     1501 tcttaaagag gctgaagagg ccgaagcctg aggatcagga tgcacccgac accaatcccg
     1561 accagaggtc ggagttgttg acgcaaccga gagcaagcgt gcggtgacaa tggacaactc
     1621 ttcgcgatac ctcgtgccga tgaccgacga agcgttcgat cctgacgccg accgtctgcg
     1681 cgaagaatgt ggcgtctttg gcattttcgg ccaccccgac gctgcggcga tcaccgcgct
     1741 tggcctgcac gcgcttcagc atcgcggcca ggaagccgcc ggcatcgtct ccttcgacgg
     1801 caagcgattt cactcggagc gccggctggg tcttgtcggc gacgccttcg cccggcgcga
     1861 ggtgatcgat cgactgccgg gcacgtcggc gatcgggcat gtgcgctact cgacgacggg
     1921 cgagacgatc atccgcaacg tgcaaccgtt gttcgctgag ctcaacgccg gcggcttcgc
     1981 catcggtcat aacggcaacc tcaccaacgg catcaagctc cgccgcgacc tcgtgcatga
     2041 gggcgccatg atgcagtcga ccaccgatac tgaggtgatc ctgcaccttg tcgcccgcgc
     2101 gaaacgcaac cggtttgtcg atcgtttcat cgagggcctg cgccagctcg agggcgctta
     2161 cgcttttgtc ggcttgacca acaagaagct cgtgggcgcg cgggacccac tcgggatccg
     2221 ccccttggta gtcggcaggc ttgacgggtg ccctatcctc gcgtcggaga cctgtgcgct
     2281 cgacatcatc ggtgcccaat acgtgcgcga cgtcgagaac ggcgaggtcg ttatcttcga
     2341 cgaagacggc gcacagtcgc acaagccgtt tccgccgatg ccggagcggc cgtgcatctt
     2401 cgagtacgtt tatttctcgc ggccggattc catcgtcggc ggccgtcacg tatacaacgt
     2461 ccgcaagacg atgggtgcgc agctggcccg ggaggctccg gcaccggccg acgtgatcat
     2521 tcccgtccca gattccggcg tgccggccgc gattggcttt gcgcaggaag ccaagatccc
     2581 ctacgaactc ggcatcattc gtaaccatta cgtcggccgc acatttattc agccgaccca
     2641 ggcgatccgc gaccagggtg ttcgcctcaa gcactcggcc aatcgcgctg tcgtcgcggg
     2701 caaacgtatc gtactcgtcg acgactccat cgtccgcggc acgacatcgc gcaagatcgt
     2761 acaaatgatg cgcgacgcag gcgcgcgcga agtccacttc cgcattgcct cgccgccgat
     2821 cacgcatccc gattactacg gcatcgatac gccggagcgc gacaagctac tcgctgcaac
     2881 ccacgatctc gagggcatgc ggaaattcat cggggcggat tcgctcgcct tcctgtctgt
     2941 ggacggaatt tatcgggcta tgggattcga aggacgcgat ccggtgcggc cccagttcac
     3001 cgaccactgc ttcacgggcg actatccgac gccgctcacc gatctcgccg ctcaggaagc
     3061 aagtccgcgg cagttgtcgc ttctggcaga agccatttaa tcgccgttat ggccggacca
     3121 atgaccggca ccaccgggcc cctttccgac cgtattgcgc tggtcacagg cgcctcgcgc
     3181 ggaattggcg cggctgtcgc gctcgaactc gcaaaggcag gcgcccatat cgtcgccgtc
     3241 gcacgcaccg ttggcgggct ggaagagctt gatgacaaga tcaaggctgc cgggagttcc
     3301 gcaaccctgg tgccgctcga catgaaggac agcgacggca ttgcccggct cgcccttgct
     3361 ctcaacgacc gctaccagcg ccttgacgtt ctgatcggca acgcgggcgt attgggcact
     3421 ctgtcgccgc tttcgcacta cgagccgaaa gactgggaca acatgatcgc ggtcaacgtc
     3481 accgcgaact ggcaactgat ccgctgcatg gacgccctgc tgcaacgctc gcaggccggc
     3541 cgcgcggtat tcctgacgtc cggcgttgca cacttggacc gcgcctattg ggggccatac
     3601 gctgcatcaa aagcagccct ggaggtcctg gtgcgcacct atgccgcgga gacggcaacc
     3661 acaaatgtgc gcgctaacct gttcggtcca ggccccacgc ggacacgcat gtatcttggt
     3721 gcctttcccg gcgtcgatcc gttgacactg ccgacacccg aagaggtcgc caaagcgatc
     3781 ctgccgcttt gcctgccggg cttcacggag tccggcaagc tctacgacta ccgcgaccgc
     3841 aagctgaaag catttcaagc tccagcctag ccgatcagtg cttgcgcttc gcgcggctct
     3901 tgtagggatt atccttctcg cgcaaggtca ggcgaatggg cgtgcccgga agctcgaatg
     3961 tctcgcgcaa agagttcaac agataccgct tgtaggcatc aggcacggca tcggcgcggg
     4021 tgcagaaaat cacaaagctg ggcggccggc tcttggcttg cgtgatgtag ttgagccgca
     4081 atgcgcgtcc tgacaccgcg ggcggcggat gtgcacttac ggcatgctcg aaccagcggt
     4141 taagtccact ggtcggaatg cgcttgttcc acacgtcata tgcatcgacg actgcctgca
     4201 tcaaccggtc gagtccttcc ccggtaaggc cggagacagc aaccagcggc acgcctttaa
     4261 gctgggtgag tttctcttcc gcctgctccc gcagcttgcc gatcgcaccc gccttttttt
     4321 cgttcaggtc ccatttgttg accgcaatga cgatggcgcg gccttcctgc tcgaccaaat
     4381 cggcgatgcg ttgatcctgc tcttcgaaag cattttcaac gtcgagcaaa acgacgacga
     4441 cttccgcgaa gcggaccgcg ttgagcgcgt cagcaaccga aagcttttcc agcttctcgt
     4501 cgacacggga gcgccggcgc aggcccgccg tgtcgtaaat ccggaatttg tggtgctgcc
     4561 agacatgctc gaccgcaatt gcgtcacgcg taatgcccgc ctcaggaccc gtcagcaacc
     4621 ggtcctcccc gatcagccgg ttgatcagcg tggattttcc agcattgggc cggcccacga
     4681 tagcaacgcg gatcggatgg gcctcgtcca ttgcaaggcc cgaggagtcc gcaccactct
     4741 gctcaggcag caccgcgcgc aatgcgtcgt agagatcgga cattccctcg tcatgctcgg
     4801 ccgagatcgg taccgggtca ccgagaccga gtgcataagc ctcgacccgt cccgcttcac
     4861 cggcggtccc ctcactcttg ttcgcgacca gaacgaccgg cttgccggac ttgcgtgcga
     4921 catccgcaaa cgcatgatcc gcgggcatcg gcccggtgcg cgcatcaatc ataaagaaga
     4981 cggcgtcggc ttgatcgatg gccgcgtctg tctgcgcttg catgcggcct gacaggcttt
     5041 ccggcttgga ttgctcgagc ccggccgtgt caatgaccct aaacgctatg tcaccgagcc
     5101 ttgcttcccc ctcacggcgg tcgcgggtga cgccgggcct gtcgtcaacg agcgccagcc
     5161 gccgcccgac cagcctgttg aagagcgtcg atttgccgac attgggacgg ccgataatgg
     5221 cgacggtgaa aggcatgaat gatggcccgg ctgcgtaacg cggcgcaggt ttttagcccg
     5281 tcacgctatt cagcgcgaga aggtgccggg ctgaggcgaa gacggccagg gagccggcgc
     5341 ggcttgcgtg ggcgcgggcc agggcgactg ggcctgttgc ggcgccggct gaggattctg
     5401 ctggggccaa ggctccattt gctgctgcgg ctgcctcgcc gcttgcgtgg actgctgcgg
     5461 cgcggtgcgc ggctttgcag cgcgcttcgg ctttggcttt ggctccggct cggcggcggc
     5521 agctttcttc tccggctcgg cctctgcctt gttctgttca gttgcgggtg cctgcaaagc
     5581 ttgcgcggtt tccggtggcg gctggtttcc cttcatgtac tcgggcggaa taccctgcgt
     5641 tacgccgggc accccgccgg gaaacaactc cttgcgctcg cccggaagct ttttcttttc
     5701 gttgagacca aatacgtcga gcttgtccgg atcgaaattc gagcacccgg caagcgcagg
     5761 cactgcggcc gcaagcactg ccagcacaac gattcgcgag atcgcgcgca tgcgttcagc
     5821 tctttccatc ggcagtgatc agcgcagaca ggacgtccgc acgcgcgcgg acgccgatag
     5881 ggctttcggc atcgcctgca atcatgtcga gatatttacg ggcagcggcg aaatcgtggt
     5941 tccgccacgc tgacaaggcg agcagttcgc gtgcagtgtg gcgaaagctc cgcgtcggtt
     6001 cggcaaccgg gtcgagccgc cgccgcatgt cagccagcgg tgccgtatcg acgagcaaca
     6061 ttcccgcgcg caccgccgca agatcctgcc agattgcccc cagcgaagca tcggcggaaa
     6121 gttcatcgaa ggccttgacg gcatccgccg gtttggattg cgccagcaca cttgccgcgc
     6181 gcatgcgcgc gagcacgcga taaccggccg gcgcttcggc tgccactttc gcaaaggccg
     6241 cctcggcctc ggcttctttg ccctgctcgc tcagggcgat cgccgcctcg aattgcgcgc
     6301 cggcaactgc agcctttttc gcgacccagt attcgtatcc gcgccagccg gcaatgccgg
     6361 caacgatcaa caccgcgagc gcgatcagat ggatgctgta gcgatcccac agctttttga
     6421 gctgctcacg gcggacttct tcgtccactt catgaaaaat atcggccacg atggttccca
     6481 acccgtctcg gcccaccccg gggccgaatt gcgccgtaac ctaccgatgt ggcgcgccta
     6541 aggcaaagcc aaagccggta atcgttaata tttcagcgcc ttagaccttc ttgctcttca
     6601 tcgggtagac gtgctccggc ccggggaaac tccgtgcgcg gacctccgcg gcataatcgg
     6661 aaatcgcctt ttcgatgccg ggtccaaggc tgccgtagcg tttgacgaat ttcgggacgc
     6721 gcggtgaaag gccgagcatg tcctcaagca ccagaatttg cccgtcgcag gcaacgctcg
     6781 caccgattcc gatggtaggg attgccacgg cggcggtgag tttgcgcgcg agcggctccg
     6841 caacggcttc aatcacgact gaaaaagcgc ccgcctgcgc gaccgcaacc gtatcctcct
     6901 cgatgcgcgc ccagtcggcc tcatcgcgcc cttgcgcccg gaacgagccg atcgcgttga
     6961 tcgcctgcgg cgtcagcccg acatgggcca tgaccggaac accgcgctca gcgagaaacc
     7021 ggatcgtttc cgccatgcgc ttgccgccct cgaccttgat cgcgccgcag ccggtttcct
     7081 tcagcacgcg cgccgcattc atgaaagcct gctctttcga ttcctcatag gaaccgaacg
     7141 gcatgtcgac aacgacaagg gcgcgccgag agccgcgcat caccgcgtgg ccctgcagga
     7201 tcatcatgtc gagtgtgacg gggacggtgg tttccattcc atgcatcacc atgccgagcg
     7261 agtcaccaac cagaatgaca tcgcaatact tgtcgacgag gcgagcggtg tgcgcatgat
     7321 atgaggtgag gcacacgatc ggctcggcgc ccttgcgcgc aagaatatcc ggtgcggtga
     7381 accgcctgat ctcgtcctga atcgacacgc attacctccc gaacaccgat acgccgatca
     7441 cgtaaggatg gaaccagtaa ccgagcgcaa gaaatatggc ggctccgaga accacgacaa
     7501 tgatgtcatt cgcccagccc tcggggacgg gcttcgctgc cgcagcaagg tcgccgcggc
     7561 gcttggctgc cgcgcgggag tagccgcccc atagcaaaaa cgaaccgaac aggataatgc
     7621 cgccgagatc accgttcgag agcaggtgcg caagcgccca ggtctttaca ctcgtcagca
     7681 tcggatgctt gagccatttg cggatacggc acgggatgaa cgcggacacg agaaagatca
     7741 c
//
LOCUS       DASSRL010000256         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1406228, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000256 DASSRL010000000
VERSION     DASSRL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406228"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..2337
                     /locus_tag="VFL49_05290"
     CDS             151..2337
                     /locus_tag="VFL49_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET7911421.1"
                     /translation="MPDGVFGTVGRLRGLLSQWLLAEVTPGRLIPWVPVAFGAGIAGY
                     FSVDREPAIWAVLPLAIGSFAVAFVARRRPFGFPFAVAMAATFTGFSVATLQTARISH
                     PVLQSSISSVILTGFVEIREERERSDRIVIRVENFAAPNVPAVPQRVRVAVRKGSAPP
                     VGSFVEIKAHLSPPMQPLRPGGYDFARDMYFQKIGASGYALGVVKIRSPPTAGGFWLR
                     YATIIDGIRESIDDRIRALLPGDKGSIASALITGKRDAISTPVNDAMYVSSLAHILSI
                     SGYHMAVVSGIVFFVIRATLALIPAFSHRRPIKKWAAFGALLAATFYLLLSGAEIATQ
                     RSYIMIAIVLSGVMLDRPTLTFRTLSVAAIVVLLIAPQAIVHPSFQMSFAATLALIAA
                     YQRSLPLTAGRDSQLGARVALWGIREVASLILASLVAGLATTPYAAYHFHRLAPYGVL
                     ANLLAMPIVSAIVMPMGILGVLSMPFGFDAPFWQLMGQGIDWMNGVALWVASLPGAVG
                     RMPAFGTGPLLLGTGCLLVICLLRTPLRWSGAVVGAGAVAWALMAPQPDVLVSGDGQT
                     AAFRGADGRLAVLHAGRDTFAVKEWLAADADARAPKDVSLSKGVTCDSIGCIGKLADG
                     RQMSAVLEIEAFVEDCMQAAIVVSEREAPGACNATLIDRAAWQSYGAVTLRWTGDRFA
                     RTVALPPGYDRPWIHRARAVSAHAEPQRRQAPREAEPRQDDLAPDD"
     gene            complement(2334..3035)
                     /gene="lexA"
                     /locus_tag="VFL49_05295"
     CDS             complement(2334..3035)
                     /gene="lexA"
                     /locus_tag="VFL49_05295"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET7911422.1"
                     /translation="MLTRKQFELLRFIHERLTEAGVPPSFDEMKDALDLRSKSGIHRL
                     ITALEERGFIRRLPNRARAIEVIKLPDSVGHGLGSARSRGFTPSVIEGDLGRVRTTSE
                     DDGGRPVAVPVMGRIAAGTPIEAIQTRSHVINMPPDLLSVGEHFALEVRGDSMIEAGI
                     LDGDIALIRKSEAADTGDIVVALIDDEEATLKRFRRRGASIALEPANTAYEVRILPPN
                     RVRIQGKLVGLFRRY"
     gene            complement(3158..3283)
                     /locus_tag="VFL49_05300"
     CDS             complement(3158..3283)
                     /locus_tag="VFL49_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911423.1"
                     /translation="MPAQDSTLMAPLAKADCLLIRQSNAPAAAAGSDCVILKLGL"
BASE COUNT          535 a         1015 c         1062 g          682 t
ORIGIN      
        1 ggtgcgctaa tatagcgcag gttgtggcat tgcgtcgggg aggcgcgtgg cgccttggcc
       61 cggagaaaaa atccatatgg acctgcccag ggtcgcgccg agacctgggc gccagcgttt
      121 gacggtgccc gccggccggc cggcctgtca ttgccagatg gcgttttcgg aacggttggt
      181 cggctgcgcg gactgctgtc gcaatggctg ctggccgaag tcaccccggg ccggttgata
      241 ccatgggtgc ccgttgcgtt cggcgcgggg atcgccggct atttctccgt cgatcgtgaa
      301 ccggccatct gggccgtcct gccgctcgcc attggctctt tcgcggtggc ttttgttgcg
      361 cgccgacggc cattcgggtt tccattcgcc gtcgccatgg cggcgacctt taccggattt
      421 tcggtggcaa cactgcagac cgcgcgcatc agtcatccgg tcctgcagtc atcgatctcc
      481 agcgtgattc tgacgggctt tgtcgagatt cgcgaagagc gggaacgcag tgaccggata
      541 gtaattcgcg ttgaaaattt cgccgcgccg aatgtccctg ctgtgccgca acgcgtgcgc
      601 gtcgcggtgc gcaagggaag cgccccgccg gtcggcagtt tcgtcgagat caaggcacac
      661 ctgtcgccac cgatgcagcc acttcgtccc ggcggctacg attttgcgcg cgacatgtat
      721 tttcagaaga tcggcgcatc gggctatgcg ctcggtgttg tcaagatcag gtctccgccg
      781 acagccggcg gattttggtt gcgatacgcg accatcatcg acggaatacg tgaatcgatc
      841 gacgaccgta ttcgcgcgct tttgcctggc gacaaaggct ccatcgcctc ggcgctgatc
      901 accggcaagc gcgatgcaat ctcgacgccc gtcaacgacg ccatgtacgt gtctagtctc
      961 gcgcatatcc tgtcgatttc cggctatcat atggcggtag tttcgggaat cgtcttcttc
     1021 gtcatccgcg cgactctggc gctgatcccc gcgttctcgc atcggcgtcc gatcaagaag
     1081 tgggccgcat ttggagcatt gctcgcggca acgttctatc ttctcttgtc cggcgccgaa
     1141 atcgcgacgc aacgctcata catcatgatc gcgatcgtgc tttccggtgt gatgcttgat
     1201 cgccccacgc tgacgtttcg cactctttcg gtggctgcca tcgtggtcct gttgatcgcg
     1261 ccgcaggcga tcgtgcaccc aagcttccag atgtcgtttg cggcgacgct ggctttgatc
     1321 gccgcctatc agcgcagttt gccgttgacc gcgggccggg acagccagct cggcgcgcgc
     1381 gtggcactat gggggattcg cgaagtcgcc agcctgattc tggcttcgct ggtggccggc
     1441 cttgcgacga caccttatgc cgcctaccat tttcatcggc tggcaccata cggcgtgctg
     1501 gcgaaccttc tcgccatgcc gatagtttcc gcgattgtca tgccgatggg cattctcggc
     1561 gtactgagca tgccgttcgg ctttgacgcg ccgttttggc aactcatggg acagggtatc
     1621 gattggatga atggcgtcgc gctttgggta gcgtcgcttc ccggcgccgt gggacgcatg
     1681 ccggctttcg gcaccggccc gcttcttttg ggcacgggtt gtctgctggt gatttgcctg
     1741 ttgcgcacgc cgctgcgctg gagcggcgcc gttgtcggcg ccggcgcagt tgcatgggcc
     1801 ctgatggcgc ctcagcccga cgtactggtg tccggcgacg gccagacggc cgcgtttcga
     1861 ggcgctgacg gccggctcgc cgttcttcac gccggccgag atactttcgc cgtcaaggaa
     1921 tggctggccg cagacgccga tgcgcgcgcc cccaaggatg tgagtctgag caagggggtc
     1981 acgtgcgatt ccatcggttg catcggaaaa ctcgccgacg gccgccagat gtcggcggta
     2041 ttggagatcg aagcgttcgt cgaggactgc atgcaggcgg ccatcgtggt gtcagaacgc
     2101 gaggcgcctg gcgcctgcaa tgctacgctc attgatcgtg ccgcatggca atcttacggc
     2161 gcggtcacgc tgcgttggac cggtgatcgt tttgcgcgga cggtcgcact tccgcccggc
     2221 tatgaccgcc catggattca tcgtgcaaga gccgtttccg cgcatgccga gccacagcgg
     2281 cgccaggctc cacgcgaggc cgagccgcgt caagacgacc tcgctccgga cgactaatag
     2341 cgacggaaga ggcccacgag cttgccctga atgcgcacac ggttcggcgg cagaatgcga
     2401 acctcgtagg cggtgttggc gggctcgagt gcgatcgacg cgccgcggcg acgaaagcgc
     2461 ttgagcgtag cctcctcgtc atcgatgagc gcgacgacga tgtcgccggt gtcagcggcc
     2521 tcggatttgc ggatcagcgc gatgtcgcca tcgagaatac cggcctcgat catgctgtcg
     2581 ccgcgcacct caagcgcgaa atgctcgccc accgacaaaa gatcgggcgg catattgatg
     2641 acatggctgc gcgtctggat tgcttcgata ggcgtgccgg cggcgatgcg gcccatgact
     2701 ggcacggcga ccggccgtcc gccgtcgtct tcggatgtcg tgcggacgcg accgagatcg
     2761 ccctcgatga cgcttggggt gaacccccgc gaacgggccg agcccaagcc atgccccacc
     2821 gaatccggca gtttgatcac ttctatggcg cgggcgcggt tcggcagccg ccggatgaat
     2881 ccgcgctcct ccagggccgt gataagccgg tgaatgcccg attttgaccg caggtcgagg
     2941 gcgtccttca tctcgtcgaa agaggggggc acaccggcct cagtcaggcg ttcgtgaatg
     3001 aaacgcagga gctcgaactg cttgcgggtc agcatctgtc tctccccgtc gggccggacc
     3061 gggtgggtcc aagccagaaa caaatcatga acggacacta tctgttcgat atgtgttccg
     3121 caactgttta atttggagtg aaaaactcga aaggccgcta caggcctagc ttaaggatga
     3181 cacagtctga gccggccgct gcggccgggg cgttcgattg ccgaatcaac aggcaatcgg
     3241 ccttggccag cggcgccatg agagtactgt cctgtgcagg cagtggagtg gcca
//
LOCUS       DASSRL010000257         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_916720, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000257 DASSRL010000000
VERSION     DASSRL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916720"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..1767
                     /gene="urtA"
                     /locus_tag="VFL49_05305"
     CDS             445..1767
                     /gene="urtA"
                     /locus_tag="VFL49_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HET7911424.1"
                     /translation="MSDMNWAAARRPQARSWLSAAAGIAFGVAATSSAMAQDTIKIGI
                     LHSLSGTMAISETTLKDTMLFLIDEQNKKGGVLGKKLEAVVVDPASNWPLFAEKAREL
                     ISKDKVSVVFGCWTSVSRKSVLPVFKELNSILFYPVQYEGEESERNVFYTGAAPNQQA
                     IPAVDYLMSKDGGSVKRWVLAGTDYVYPRTTNKILEAYLKAKGVKDEDIMINYTPFGH
                     SDWQTIVADIKKFGSAGKKTAVVSTINGDANVPFYKELGNQGVKATDIPVVAFSVGEE
                     ELAGLDTKPLVGHLAAWNYFESIKNPANAEFIKKWQASTKNPKRVTNDPMEAHVIGFQ
                     MWVKAVEKVKSTDPDKVIDALPGIEAENLTGGTSKMLANHHITKPVFIGEVRGDGQFD
                     VVWKTKGLVPGDAWSDFLPGSKDLEADWVTLKCGNYNKVTRKCGGQAS"
     gene            1825..3444
                     /gene="urtB"
                     /locus_tag="VFL49_05310"
     CDS             1825..3444
                     /gene="urtB"
                     /locus_tag="VFL49_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET7911425.1"
                     /translation="MKCMHGFHDRALALLFAIGLLLFGATLANAGPYEDALAGLTTDS
                     FGDTADAIEALTASGDPRTGPVLEALKDQRLLYSAETRRVFVKDRSDRLTDAATGVPV
                     AAAPADADNVRLNNRVRGMLDAALGSLTLMAKEPGRRLDAAQAVFKSRDAGALPNIDK
                     ALEKETDTRVRKALTEARAAILLSSAETSPEDKIGAIAVIRDRGDQDALGLIDGLPAD
                     LPPAVRQAAASASAKIQGNLALWSAVQNAWYGLSLGSVLLLAAIGLAITFGVMGVINM
                     AHGEMVMLGAYTTFVVQEYIRAYNPALFDYSLIIAVPLAFAVAGAVGVAIERGIIRFL
                     YGRPLETLLATWGLSLILQQAVRTVFGPTNKEVGNPSWMSGAFELGGLTITYNRLWII
                     CFALAVFVALLAMLRLTRLGLEMRAVTQNRAMAAAMGIRTARIDALTFGLGSGIAGIA
                     GVALSQIDNVSPNLGQGYIIDSFMVVVFGGVGNLWGTLVGALTLGIANKFLEPYAGAV
                     LGKIAILVLIILFIQKRPRGMFALKGRAVEA"
     gene            3445..4590
                     /gene="urtC"
                     /locus_tag="VFL49_05315"
     CDS             3445..4590
                     /gene="urtC"
                     /locus_tag="VFL49_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HET7911426.1"
                     /translation="MGAFPLTRLLDRSAAVFLILLAAFAVVIPTLNLLMPASSGLHVS
                     TYLVALFGKYICYAMLALSIDLIWGYAGILSLGHGAFFALGGYAMGMYLMRQIGTRGV
                     YANPILPDFMVFLNWHELPVYWYGFNHFAYAALMIVLAPGLLAFVFGWFAFRSRVTGV
                     YLSIITQAMTYALMLGFFRNNFGFGGNNGLTDFKDILGFNVQAETTRNALLVASCLAL
                     AICFLICRAVVTSKFGKVLVAMRDAETRTRFLGYRVEAYKLFVFTLSAGMAGVAGALY
                     VPQVGIINPSEFAPGNSIEAVIWVAVGGRGTLTGAVLGAFVVNYAKTFFTTGWLASYW
                     LFVLGGLFIAVTLLLPRGIVGTYEWWRARRAEKQTIPIAAGLPHAAE"
     gene            4597..5352
                     /gene="urtD"
                     /locus_tag="VFL49_05320"
     CDS             4597..5352
                     /gene="urtD"
                     /locus_tag="VFL49_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HET7911427.1"
                     /translation="MQLKTTSAMLYLDGISVSFDGFKALNELSFVIEPGEMRAIIGPN
                     GAGKTTMMDVITGKTRPDNGDVYFEQGSYDLSTLDETEIAELGIGRKFQKPTVFDMHT
                     VEDNLLLALKNDRSVRAALFWEDSSAQRDRINQILHIIRLGSARARVAGSLSHGQKQW
                     LEIGMLLAQEPKLLLVDEPVAGMTDAETMQTAELLKEINRDKTVIVVEHDMAFVRELG
                     VKVTCLHEGSVLAEGSIDQVSQNERVVEVYLGR"
     gene            5464..>5618
                     /locus_tag="VFL49_05325"
     CDS             5464..>5618
                     /locus_tag="VFL49_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7911428.1"
                     /translation="MLEVSNIDLHYGAAQALRGISLKAEPGKVTCVLGRNGVGKTSLL
                     RAIMGQHP"
BASE COUNT         1028 a         1782 c         1728 g         1080 t
ORIGIN      
        1 ggcgaactgc tgatcagctt gaggatttca gcggtcgcgg cctgctgatg cagcgcctcg
       61 tcgcgctcgc gcgtgagctg cgcaaccgtt gaatctttgc caatggtctg acggcgcgtg
      121 gcttttcgcg cattgcgtgc gttggccgtc ttgcgaccgg tcttgcgccg cttcattccc
      181 taacgcccca tgtcggacat ggtacgcagc aaacatgcgc gcaaaaaggg ggctgcgcgc
      241 agcctgcctc cgaagaggca atctgcctaa ataatagcgc gtgtgccgct caggcgatgg
      301 gcaggctgcg cagcaaattg gcgaacgcgc caaataaccc agtcaaatcc gtcagatgac
      361 ctgtctggca cgcgccttgc gaataggtgt ttgcggagcc gggaccgggc ccgattgcgc
      421 gtgcgtcaaa caggagagag gcaaatgtcg gacatgaatt gggcggccgc gagacggccg
      481 caggcacgca gctggctgtc ggccgccgcg ggcatcgcat tcggagttgc agccacgtcc
      541 tcggcgatgg cccaggatac gatcaagatc ggcattcttc attcgctgtc cgggacgatg
      601 gcgatcagcg agaccacgct gaaagacacg atgctgtttt tgatcgacga gcagaacaag
      661 aaaggcggcg ttctcggcaa gaaactggag gcggtggtcg ttgaccctgc gtccaactgg
      721 ccgcttttcg ccgaaaaggc gcgcgagctg atctcgaaag acaaggtctc ggtcgtgttc
      781 ggctgctgga cctcggtgtc gcgcaaatcg gtgctgccgg tgttcaagga gctcaattca
      841 attctcttct acccggtgca atatgagggc gaggagtccg agcgcaacgt cttctacacc
      901 ggcgctgcgc cgaaccagca ggcaatcccg gccgtcgact acctgatgag caaggacggc
      961 ggatcggtga agcggtgggt gctcgcgggc actgactacg tctatccgcg caccaccaac
     1021 aagatcctcg aggcctatct gaaggcgaaa ggcgtcaagg acgaggacat catgatcaat
     1081 tacacgccgt tcgggcattc cgactggcag accatcgtcg ccgatatcaa gaagttcggc
     1141 tcggctggca aaaagaccgc cgtggtgtcg accatcaacg gcgacgccaa cgtgccgttc
     1201 tacaaggaac tcggcaacca gggcgtcaag gcaaccgaca ttccggtggt ggcgttctcg
     1261 gtcggcgaag aagaactcgc cggcctcgac accaagccgc tggtcggcca tctcgcggcg
     1321 tggaactact tcgagtcgat caagaatccc gccaacgccg agttcatcaa gaagtggcag
     1381 gcttccacga agaacccgaa gcgcgtcacc aacgatccga tggaagcgca tgtcatcggc
     1441 ttccagatgt gggtgaaggc cgtcgagaag gtcaaatcaa ccgatccgga caaggtgatc
     1501 gacgccctgc ccggcatcga ggctgaaaac ctgacaggcg gcacctcgaa gatgctggcg
     1561 aaccaccaca tcaccaagcc ggtgtttatc ggcgaagtcc gcggcgacgg ccagttcgac
     1621 gtcgtgtgga agaccaaggg tctcgtgccg ggcgacgcct ggtccgattt cctgcccggc
     1681 tcgaaggatc tcgaggctga ctgggtgacg ctgaagtgcg gcaactacaa caaggtgacc
     1741 aggaagtgcg gaggccaggc ctcctgaacg gacggcggcg gggcggcggt caatcgccgc
     1801 ctcccgtcgc gcatttcaag ccggatgaaa tgcatgcatg gctttcacga tcgcgcgctg
     1861 gcgcttttat tcgccatagg ccttctgctt tttggcgcga cattggccaa tgccggcccc
     1921 tacgaggacg cgctggcggg cctcacgacc gattccttcg gcgacacggc cgacgccatc
     1981 gaggcgctga ccgcaagcgg cgacccgcgc accgggccgg tccttgaagc gctcaaggat
     2041 cagcggttgc tttattccgc agagaccagg cgcgtcttcg tcaaggacag atccgacagg
     2101 ctgacggatg cagcgaccgg cgtgcccgtg gcggcggcgc cggccgatgc cgacaacgtc
     2161 cggctgaata atcgcgtgcg cggaatgctc gacgcggcgc tcggctcgct cacgctgatg
     2221 gcgaaggagc cgggccgccg gctcgacgcc gcccaggcgg ttttcaagtc ccgcgacgcc
     2281 ggcgcactgc ccaatatcga caaggcgctc gagaaggaga cggacacgcg cgtcagaaag
     2341 gccctgaccg aagcgcgcgc ggcgatcctg ctctcctccg ccgagacaag ccctgaagac
     2401 aagatcggcg ccattgccgt catccgcgac cgcggcgacc aggacgcact tggcctcatt
     2461 gacggcctgc cggccgatct tccgccggcc gtgcgccagg ctgcggcgtc agcgagcgcg
     2521 aaaattcagg gcaatcttgc gctctggagc gccgtgcaga acgcctggta tggcctttcg
     2581 ctcggctccg tcctgctgct cgcggcgatc ggcctcgcca tcaccttcgg cgtgatgggc
     2641 gtcatcaaca tggcgcatgg cgagatggtg atgctcggcg cgtacacgac gttcgtcgtg
     2701 caggaataca tccgcgctta caatcccgcg ctgttcgact attcgctgat cattgcggtg
     2761 ccgctggcct tcgccgtcgc cggcgccgtc ggcgtcgcga tcgagcgcgg catcatccgc
     2821 tttctctacg gtcggccgct cgaaacgttg ctcgccacct ggggcctgtc gctgatcctg
     2881 cagcaggccg tacgcacggt cttcggcccg accaacaagg aggtcggcaa cccctcgtgg
     2941 atgagcggcg cgttcgagct cggcggcctc accatcactt ataaccggct ctggatcatc
     3001 tgctttgcgc tggcggtttt cgttgccttg ctcgccatgc tgcgcctgac ccggctcggc
     3061 cttgaaatgc gcgccgtcac gcaaaaccgc gccatggcgg ccgccatggg tatccgcacc
     3121 gcgcgcatcg acgccctcac cttcgggctc gggtccggta tcgccggcat cgccggtgtc
     3181 gcgctgtcgc agatcgacaa tgtctcgccc aacctcggac aaggctacat catcgactcg
     3241 ttcatggtcg tggtgttcgg cggggtcggc aatctgtggg gcacgctggt cggcgctttg
     3301 acgctcggca tcgccaacaa attcctcgag ccctatgccg gcgccgtcct cggcaagatt
     3361 gcgatcctgg tgctcatcat ccttttcatc cagaaacggc cacgcggcat gtttgcgctc
     3421 aagggccgcg cggtggaggc ctagatgggc gccttcccgc tcacacgcct gctcgaccgt
     3481 tccgcggccg tattcctgat attgctcgcg gcctttgccg tcgtcattcc gacgctcaac
     3541 ctgctgatgc ccgcaagctc cggactgcac gtatcgactt atctcgtcgc gctcttcggc
     3601 aagtacatct gctacgcgat gctggcgctt tcgatcgacc tgatctgggg ctatgccggc
     3661 atcttgtcac ttggccacgg cgcatttttc gcgctcggcg gatacgccat gggcatgtac
     3721 ctgatgcggc agatcggcac gcgcggcgtt tatgccaatc cgatcctgcc ggacttcatg
     3781 gtgttcctga actggcatga actgccggtc tactggtacg gcttcaatca cttcgcctat
     3841 gcggcgctga tgatcgtcct tgcgcccggg ctgctcgcct tcgtgttcgg ctggtttgcg
     3901 ttcaggagcc gcgtcaccgg cgtctacctg tccatcatca cacaggcgat gacctacgcc
     3961 ttgatgctcg gcttcttccg caacaatttc ggtttcggcg gcaataacgg cctcaccgac
     4021 ttcaaggata tcctcggctt caacgtgcag gccgaaacga cacgcaacgc cctgctggtc
     4081 gcatcgtgcc ttgcactcgc catctgcttt ctcatctgcc gcgccgttgt gacctcgaaa
     4141 ttcggcaaag tgctggtggc gatgcgcgac gccgagacgc gcacgcgctt tctcggctac
     4201 cgcgtcgaag cctacaagct gttcgtgttc acgctgtcgg ccggcatggc gggcgtcgcc
     4261 ggcgcgctct acgtcccgca ggtcggcatc atcaacccga gcgagtttgc cccgggcaat
     4321 tcgatcgagg cggtgatctg ggtcgccgtc ggcggccgcg gcacccttac cggagcggtg
     4381 ctcggcgcct tcgtcgtcaa ctacgccaag accttcttca ccacgggctg gctcgcgtct
     4441 tactggctct tcgttctggg cggcctgttc atcgcagtga cgctgctgct gccgcgcgga
     4501 attgtcggca cctatgagtg gtggcgggcg cgccgggccg aaaagcagac cattccgatc
     4561 gcggccgggt tgccgcatgc ggcggagtga gatgacatgc agctgaaaac aacctccgca
     4621 atgctctatc tcgacggcat cagcgtgtcc ttcgacggct tcaaggcact gaacgagctg
     4681 tcgttcgtca tcgagcctgg cgaaatgcgc gccatcatcg gcccgaacgg tgccggcaaa
     4741 accacgatga tggatgtcat cacgggcaag acccggcccg acaacggcga tgtctatttt
     4801 gagcagggct cctacgacct gtccacgctc gacgaaaccg agatcgccga gcttggcatc
     4861 ggccgcaagt ttcagaagcc gacggttttt gacatgcata ccgtcgagga caatctgctg
     4921 ctggccctca aaaatgaccg cagcgtgcgt gcggcgttgt tctgggaaga ttcatcggcg
     4981 cagagggacc gcatcaatca gatcctgcac atcatccggc tcgggagcgc ccgcgcgcgc
     5041 gtcgccggca gcctgtcgca cggccagaag caatggctcg agataggcat gctgctcgcc
     5101 caggagccca agctgcttct tgtggacgag ccggtcgccg gcatgactga tgccgaaaca
     5161 atgcagaccg ccgagcttct gaaagagatc aaccgcgaca agacggtcat tgtggtcgag
     5221 cacgacatgg ctttcgtgcg tgagctcggc gtaaaggtca cctgcctgca cgaaggctct
     5281 gttctcgcag aaggctcgat cgaccaggtc tcgcagaatg aacgtgtggt ggaggtgtat
     5341 ttggggagat agcaaatagg gcgtagcgaa tagcttctcc ggtcgtttcc attcactact
     5401 cgctactcgc tattcgctat tccctattcg ctacttccct attcgccctg gcggagagaa
     5461 ataatgctag aggtttcaaa catcgatctc cactacggcg ctgcccaggc gctgcgggga
     5521 atctcgctca aggccgagcc gggcaaggtg acctgcgtgc tgggccgcaa tggtgtcggc
     5581 aaaaccagcc tgttgcgcgc gatcatgggc cagcatcc
//
LOCUS       DASSRL010000258         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1196733, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000258 DASSRL010000000
VERSION     DASSRL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196733"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VFL49_05330"
     CDS             complement(<1..1307)
                     /locus_tag="VFL49_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7911429.1"
                     /translation="MARSKIDRRRFLAGVALAGAAGASSEGAKAATASVIEKTPRPSA
                     LRPSAAVAAAETGTPQVSMKSAGVPGSDFMVDVIKSLDIPYLPSNPAQSFRGLHESLI
                     DYGGNKKPEFLTCTHEETSVAMAHGYFKIAGKPLLVLCHGTVGLQHAAMAIYNAWCDR
                     VPVIAMGGTDLDASRRPPGVPTFHSAQDICALVRDFTKWDDQPVSLQHFAQSFVRAYK
                     LAMTPPHEPVALALNAELQEMTAHDRARLSIPRYVPTAPPQGDLNAVREAARLLANAE
                     HPVIVADKCARTANGVRLLVELAETLQAPVIDQKGRMNFPNTHHLSQNARGRALIGEA
                     DVILGLELSDYWGTVNGFIDNGEIDGTGLQESHIKPSTKLISISSILLNTKSNYQDFQ
                     RLQVVDIEMAADAEATLPALIEAVRQAQPSSRKAAIEARGAAM"
     gene            complement(1404..2474)
                     /locus_tag="VFL49_05335"
     CDS             complement(1404..2474)
                     /locus_tag="VFL49_05335"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7911430.1"
                     /translation="MARFSSVFMLTLPVAAAFLVTPALAQRPLPPPGIYQEQLPPVMG
                     NYDDDDLYPATPRGPRNGSRGSNRVVPYPDDDPSPPPTGLFDSSRGHQLDTRRQPPGA
                     TQAPVAATSDPEAIRPPGQVGVAPNAAPQPGQSPNTVMSLPPEDQPETGQPKELPPNL
                     KKQLVDFTTKEPAGTIIVDTANTYLYLVLGNGKAMRYGIGVGRDGFTWTGTERISRMK
                     EWPDWFPPSEMIERQPYLPRMMAGGPGNPLGARALYLGHTLYRIHGTNQPSTIGSFVS
                     SGCIRLLNEDIEDLYSRVQVGTRVVVLPGKAPEHIANTGAQPAAAGAAPAAARANAAP
                     GSGGGPGNSAAAVTSSPLPPVR"
     gene            complement(2596..2979)
                     /locus_tag="VFL49_05340"
     CDS             complement(2596..2979)
                     /locus_tag="VFL49_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7911431.1"
                     /translation="MDKALYDRGMTMRRKVLGDEYVDRAVANTDDFNRQFQELLTTYC
                     WGAVWGDDALKPRERSILNLGMIACLGRMHEFEAHFRGALRNGLTPNELSAILRQIAV
                     YCGFPAAVDCHRIAKQVLAAEAKKT"
     gene            3128..3739
                     /locus_tag="VFL49_05345"
     CDS             3128..3739
                     /locus_tag="VFL49_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HET7911432.1"
                     /translation="MRISSPRIAYLALSLFVVGFAAYAIAPKAAAQDAGWTVLFDGKN
                     LDQWNQVGESNWHIVDGDVVADKGGKDAGYLVSKQSYKNFIVRVEFWPSDDANSGIYF
                     RCLDPKKITDRTCYEANIFDQRKDPSYGTGAITRYVEVNPMPKAGGKWNIYEVTVNGR
                     DITAVLNGQTTAKLRNGMFDEGPIALQHGAGSIKFRKVEVKPL"
     gene            3769..>4471
                     /locus_tag="VFL49_05350"
     CDS             3769..>4471
                     /locus_tag="VFL49_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7911433.1"
                     /translation="MEVAIVGGGICGLSLALNLKERGIACRVYERAPQLKELGVGITL
                     LPHAMREFTALGLAQELLKAGIENGESRFYNRFGQIIYKESRGKLAGYQYPEVCIHRG
                     RLHMILYRAALARLGADAIRTDHECTGAEQDERGVTLHFKNRGSVRADIAVDCEGINS
                     TIRRQFYPSDKVAFAGINTWRGVTRRKPIFDGRTYMRVGSILTGKIVIYPIVDNIDGA
                     GHQLINWMAEIKRDTF"
BASE COUNT          883 a         1358 c         1393 g          837 t
ORIGIN      
        1 ttcatcgccg cgccgcgcgc ctcgatggct gccttgcggc tcgatggctg cgcctgcctt
       61 accgcctcga tcagcgcagg aagcgtcgcc tcggcatcgg ccgccatttc gatgtccacg
      121 acttgcaatc gctgaaagtc ctggtagttc gactttgtat tgagcaggat cgagctgatg
      181 ctaatcaatt tggtgctggg cttgatgtgc gattcctgga gaccggtgcc gtcgatctcg
      241 ccgttgtcga tgaagccgtt aaccgtgccc cagtagtccg acagttcgag gccgaggatc
      301 acgtcggctt cgccaatcaa cgcacggcca cgcgcgttct gggaaaggtg gtgagtattg
      361 ggaaagttca tccggccctt ctggtcgatg actggggcct gtaacgtctc ggccagctca
      421 accagcagcc tgacgccgtt ggccgtgcgc gcgcatttgt ccgcaacgat cacagggtgt
      481 tcggcgttcg cgagcagacg ggcagcttcg cgcaccgcat tgagatcgcc ctgcggcggc
      541 gcggtcggca catagcgcgg aatgctcagc ctggcacggt cgtgcgccgt catttcctgc
      601 agctcggcat tgagcgccag cgcgaccggt tcgtgcggcg gcgtcatcgc gagcttgtaa
      661 gcgcgcacga acgattgtgc gaagtgctgg agcgataccg gctggtcgtc ccatttggtg
      721 aaatcacgta caagcgcgca aatatcctgc gccgagtgga aggtcggcac gccgggcgga
      781 cgcctggagg cgtcgagatc ggtgccgccc atcgcgatga ccggaacgcg gtcacaccat
      841 gcattgtaga tcgccattgc cgcatgctgg aggcccacgg tgccgtggca cagaaccagc
      901 aggggcttgc ctgcaatctt gaaatagccg tgcgccatcg cgaccgacgt ttcttcatgc
      961 gtgcaggtga gaaattcagg cttcttgttg ccgccatagt cgatcagcga ttcatggagt
     1021 ccacgaaagc tctgcgccgg attgctcggc aggtagggaa tgtcgagcga cttgatcacg
     1081 tcgaccatga agtcggaccc cggcacgccg gcggatttca tcgaaacctg cggcgttccg
     1141 gtctccgcag cggcgaccgc agccgacgga cgcaatgccg aagggcgcgg cgttttttct
     1201 atgacggaag cggttgcggc tttggcgcct tccgatgacg cgcccgccgc accggcgagc
     1261 gcaacacccg caagaaatct gcgtcggtcg atcttggatc gtgccatcgc cgcctcccct
     1321 tgcatgactg cagctactgc cgagaacagc agcgccgaga caatgttagt gcaggggcta
     1381 ttgaggcgct accggcccgg cggttaacgc accggcggta gaggggatga ggtaacggcg
     1441 gcagcgctgt tgcctggccc gccacccgag ccaggcgcgg cgttggcccg agcggcggca
     1501 ggcgccgcgc ctgctgccgc cggttgcgca ccggtattgg cgatatgctc aggcgccttg
     1561 ccgggcagca cgacgacccg cgtgcccacc tgaacacggc tgtaaagatc ctcgatgtcc
     1621 tcattgagaa gacggatgca gcccgacgat acgaatgagc cgatcgtcga aggctggttg
     1681 gtgccgtgaa ttcgatagag cgtgtggccg agatagagcg cgcgcgcgcc gagcggattg
     1741 ccgggaccgc ctgccatcat ccgtggcaaa tagggctggc gctcgatcat ttcagacggc
     1801 gggaaccagt ccggccattc cttcattcgt gaaatgcgct cggtgcctgt ccaggtaaat
     1861 ccgtcccggc caacgccaat gccgtaacgc atggccttgc cgttacccag cacgagatag
     1921 agataggtgt tggcggtatc gacaatgatc gtgccggccg gctctttggt cgtgaagtcg
     1981 accagctgtt ttttaaggtt cggcggcaat tccttcggct gccctgtttc cggttgatcc
     2041 tccggcggca aggacatcac ggtattgggg gactggcctg gctgcggtgc cgcgttaggc
     2101 gccacgccga cctggcccgg cggacgaata gcctccggat cggacgttgc cgcaacagga
     2161 gcctgcgtcg cccccggcgg ctgccgacgc gtgtcgagtt gatggccgcg ggatgaatcg
     2221 aagaggccgg tgggcggcgg cgacggatcg tcgtccggat aaggaacgac cctattcgat
     2281 ccacggcttc catttcgcgg gccgcgcggc gtagccggat agaggtcgtc atcgtcatag
     2341 ttgcccataa ccggcggaag ctgctcctga tagattcccg gcggcggcaa aggccgctgt
     2401 gccagtgcgg gggtgaccaa aaaggccgcc gcaaccggca aagtcagcat aaaaacggaa
     2461 gagaatcggg ccattatcca cctagagccg agaaaaggag tgccccgccg agccgcgcct
     2521 tcattgctcc gggatgaagg cacattcaag accacaatac gactatctca ttagcgtgat
     2581 tctgccgctc caatatcagg ttttcttggc ttctgcggcg agaacctgct tggcgatccg
     2641 atggcagtcg accgccgccg ggaacccgca gtaaacggcg atctgccgca aaatagccga
     2701 aagctcattt ggcgtgagcc cgttgcgaag tgcaccgcgg aaatgcgctt caaactcatg
     2761 cattcggccg aggcaggcga tcatgccgag gttgaggatg ctgcgctcgc gcggcttgag
     2821 agcgtcgtcg ccccagacgg cgccccagca ataggtcgtg agcaattcct ggaattgccg
     2881 gttgaaatcg tcggtattgg cgaccgcgcg gtctacgtat tcgtcgccca ataccttgcg
     2941 acgcatcgtc attccgcggt cgtaaagtgc cttgtccatg ggatcctccg ttttgtcagg
     3001 gagccggtgc cgtatcagcc catgttgaca gacaggaacc gatttcgtcc attcatacgg
     3061 tcccggcccg gccagcaaag aaccggatcc aacaaaaatc ccgccgggcc gattgggagg
     3121 aaacgccatg cgcatttcgt cgccacgcat cgcctatctc gcgctgtcgc tttttgtcgt
     3181 gggatttgct gcatacgcca ttgcacccaa ggcagcagcc caggatgccg gctggaccgt
     3241 cctgttcgac ggcaaaaacc tcgaccaatg gaatcaggtc ggcgaatcca attggcacat
     3301 tgtggatgga gacgtcgtcg ccgacaaagg cggcaaggac gcgggctacc tcgtaagcaa
     3361 gcagtcctac aagaatttta tcgtccgcgt ggaattctgg ccgagcgacg atgccaacag
     3421 cggcatttat ttccgctgcc tcgaccccaa aaagattacc gaccgtacct gctacgaggc
     3481 caacatcttc gatcagcgca aggacccgag ctacgggacc ggcgccatca cccgttacgt
     3541 cgaggtcaat ccgatgccga aagccggcgg caaatggaat atctacgaag tcacggtcaa
     3601 tggccgcgac atcacggccg tgctcaacgg acagacgacc gcaaagttgc gtaacggcat
     3661 gttcgatgag ggcccgatcg ccctccaaca cggcgctggt tcgatcaagt tccgcaaggt
     3721 cgaagtcaag ccgctataag acgacgaaga acagagcgcc gtagggcaat ggaagtcgct
     3781 atcgtcggcg ggggcatctg cggactgtcg ctcgcactca atctcaagga gcgtggcatt
     3841 gcctgccgcg tctatgagcg tgcgccgcag ctcaaagaac ttggcgtagg catcacgctg
     3901 ctgccgcatg ccatgcgcga gttcaccgcg ctcggtcttg cccaagaact gctcaaggcc
     3961 ggcatcgaga acggggagag ccgcttctac aatcgtttcg gccagatcat ctacaaggaa
     4021 tcgcgcggga agctggcagg ctatcagtat ccggaagtct gtatccatcg cggccgcctc
     4081 cacatgatcc tgtatcgggc ggcgctggcg cgtctgggcg ccgacgcgat ccgcactgac
     4141 catgaatgca ccggcgcgga gcaggacgag cgcggggtaa ccctgcactt caagaatcgc
     4201 ggcagcgtgc gcgcggacat tgcggtcgac tgcgagggca tcaattcaac catccgcagg
     4261 caattctacc cttcggacaa agtcgcgttt gccggcatca acacgtggcg cggagtcacg
     4321 cgacgcaagc cgatcttcga cggccgcacc tatatgcggg tgggctccat tttgacgggc
     4381 aagatcgtca tttaccccat cgtcgacaac atcgacggcg ccggacacca gctgatcaac
     4441 tggatggccg agatcaagcg cgatacgttc g
//
LOCUS       DASSRL010000259         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_444312, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000259 DASSRL010000000
VERSION     DASSRL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444312"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VFL49_05355"
     CDS             <1..1496
                     /locus_tag="VFL49_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7911434.1"
                     /translation="FAPKLQAPRSAPRFQKYDREALAKLAESATFAAPGSGAGATGFD
                     STNSRKARSKVKTKNAANAQAIAPGVPAVPAPSAYDKAADITASVPAGMPGAPPVQIG
                     PIRTPLKKRKAHTEPEDPYTPLGIRAGAFDLFPAVELIGGYNTDPGQAPNGKAAWFYT
                     VVPELRAQSDWSRHELKADLRGSYIGYTPDETPSLNRPYFNGRVDGRIDVTHDTRIDL
                     AGRVLVSTDNPGSPNLQAGLAKLPIYVTGGGTIGLGQRFNRFDLSIKGDVQRTVYQQS
                     LLTDGSTVSNDDRNYDQYAGTLRGGYELSPGLSPFAEIVADTRVHDLNTDSSGFQRNS
                     NGLTGKVGAKFELTRLLTGEAALGYTRRVYEDPRLEDISGPIGDVSLIYTASALTTVK
                     VLGRSTIGESTIPGVSGVFYRDVGVQVDHSFRRWLVGSLKLGFGVDDYVGNDRLDKRY
                     SAGVGVTYKLNRSLQIKGEFRQDWLQSNAPNADYTASIVMLGVRVQR"
     gene            complement(1497..2375)
                     /gene="galU"
                     /locus_tag="VFL49_05360"
     CDS             complement(1497..2375)
                     /gene="galU"
                     /locus_tag="VFL49_05360"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET7911435.1"
                     /translation="MPIRKAVFPVAGLGTRFLPATKAMPKEMLPVVDRPLIQHVVDEA
                     RQAGIEHFIFVTGRNKSVIEDHFDRQFELELTLTERGKKAELNLLSQDLPEAGTVSFT
                     RQQSPLGLGHAVWCARELVGHEPFALLLPDVLVQHTHGCLTQMIDAAKDLDGNANIIA
                     VEEVPADRVHMYGVVGVGAAKGKAFSITRMVEKPKASEAPSNLSITGRYILQPQIFEL
                     LEKQAAGAGGEIQLTDAMVALMRTQPFYGLKFEGRSFDCGSKIGFLSANIAYGLQRDD
                     IAPELRAEMRRLLDGK"
BASE COUNT          478 a          716 c          720 g          479 t
ORIGIN      
        1 tgtttgcgcc gaagctgcag gcgccgcgca gtgctccgcg atttcagaaa tacgatcgtg
       61 aggctctggc gaagctcgcc gaatccgcaa catttgcggc ccctggctcg ggagccggcg
      121 cgaccgggtt tgactccaca aacagccgca aggcccggtc gaaagtcaaa acgaaaaatg
      181 ccgcgaatgc tcaggcaatc gcgcctggcg ttcctgcggt gccggcaccc tctgcatacg
      241 acaaggcagc ggacataacg gcgagcgtgc cggcaggcat gcccggagcg ccgccggtac
      301 agattggacc gatccgcacg cctctgaaga aacgaaaggc gcacaccgag cccgaggatc
      361 cttacacccc gttgggcatc cgcgcgggcg cgttcgatct tttccccgct gtcgagttga
      421 tcgggggcta caatacggat ccagggcagg cgcccaatgg caaagccgcc tggttctata
      481 ccgtcgtacc tgagttgcgt gcgcagtcgg actggtcccg ccacgaactc aaagccgatc
      541 tgcgcggcag ctacatcggt tacacccccg acgaaacgcc ttcgttgaac cggccttact
      601 tcaatggccg ggtcgacggc cgcatcgatg ttacgcacga tacccgcatc gacctcgcgg
      661 ggcgcgtcct ggtgtcgacc gacaatcccg gcagtccgaa tttgcaggcc gggctcgcca
      721 agcttccgat ctatgtaaca gggggcggca ccattgggct tggccaacgc ttcaatcgtt
      781 tcgacctgtc gataaagggc gatgtgcaac gcaccgtgta ccagcaatct ttgttgaccg
      841 acggttcgac cgtctccaat gacgatcgca actatgatca atatgcgggg actttgcgcg
      901 gcggctatga gctgtcgccc gggttgtcac cctttgccga gattgtcgca gatacgcgtg
      961 tgcacgatct caacacggat tcttccggtt tccagcgaaa ttcaaatggt ctgaccggaa
     1021 aggtcggtgc gaagttcgag ctcacgcgcc ttctcacggg cgaagccgca ctcggctata
     1081 cgcgccgcgt ctacgaagat ccgcggctgg aagatatcag cgggccgatc ggcgatgttt
     1141 cgcttatata caccgcgagc gcgctgacga ccgtaaaggt gctcggacga tcaacaatcg
     1201 gggaatcgac gattcccggc gtatccggcg tattttatcg cgacgtcgga gtgcaggtcg
     1261 accattcgtt caggcgttgg ctggtcggaa gtctcaagct gggttttggc gtcgacgact
     1321 atgtggggaa tgaccggctc gacaagcgct actcggcagg cgtgggcgtg acctacaagc
     1381 tcaaccgctc tctccagatc aaaggtgagt ttcggcaaga ttggctgcaa tcgaatgcgc
     1441 cgaacgccga ctacacggcg agcatcgtga tgcttggtgt gcgcgtgcag cgctagctac
     1501 ttgccgtcca gcaagcggcg catttcggcg cgaagctcgg gcgcaatatc gtcgcgctgc
     1561 agtccgtaag cgatgttggc cgagagaaat cctattttcg agccgcagtc gaagctgcgg
     1621 ccttcgaatt tcaatccata aaacggctgc gtgcgcatca atgccaccat cgcgtcggtg
     1681 agctgaattt caccgcccgc acctgccgcc tgcttttcca gaagttcgaa gatctgcggc
     1741 tgcaggatgt agcgtcccgt gatcgaaaga ttggaagggg cttcactggc cttcggtttt
     1801 tccaccattc tggtgatcga aaatgccttt cccttcgcgg cgccgacgcc aaccacgcca
     1861 tacatatgga cacggtcggc cgggacttcc tcgaccgcga tgatattggc attgccgtca
     1921 aggtctttgg cggcgtcgat catctgcgtc aggcaaccgt gggtgtgctg gaccagcacg
     1981 tcgggcagaa gcaatgcaaa cggctcatgg ccgacaagtt cgcgggcgca ccatacggcg
     2041 tgacccaggc cgagcggtga ctgctgccgc gtgaaactga ctgtccccgc ctcgggcaag
     2101 tcttgcgaca acaagttgag ttcggccttc ttgccccgct ccgtcaaggt taattcgagt
     2161 tcgaactggc gatcgaaatg atcctcgatc acgcttttgt tccggcccgt tacgaatatg
     2221 aaatgttcga tgccggcctg gcgggcttca tcgaccacat gctggatcaa tggccggtcg
     2281 acgaccggaa gcatctcctt gggcatggcc ttggtggccg gcaggaaacg ggtgccgaga
     2341 ccggcaacag ggaagacagc cttccggatg ggcaggttca tgagggcctc ttg
//
LOCUS       DASSRL010000260         5561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_601670, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000260 DASSRL010000000
VERSION     DASSRL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601670"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VFL49_05365"
     CDS             <1..805
                     /locus_tag="VFL49_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7911436.1"
                     /translation="TVPCTKVTGRWLFPKAELDHWLVSSVSRPEGMVRPDAAPIVSGS
                     HDPLFEWALRESGSGLAMLAVGSEAGFSRFTTGETIAAAIHLHALEDLEADANVAALR
                     GRNELQDAVMIAFCRREQGFVVAPGNPLKLASIEDIRKKRARIAMRPRGAGAQLLLLA
                     LLHRADIAFDQLVSAAPVCPTGPDIAQAIRAGRADTGIATRGVANAAGLDFTPIVWER
                     FDLVMRQRDYFRTPLQSLLKFFHSPDFAARARELGGYDLNTAGTVRLVN"
     gene            805..1185
                     /locus_tag="VFL49_05370"
     CDS             805..1185
                     /locus_tag="VFL49_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET7911437.1"
                     /translation="MTDAQVWPTELRLHKDRKALTVTFDSGERFELPAEYLRVKSPSA
                     EVQGHSEEERKTVPGKRNVEILEVLPIGNYAVRLVFDDMHSTGIFGWDYLHDLGRNHA
                     AYWQDYLDELAARNMTRDPPARRS"
     gene            complement(1224..1631)
                     /locus_tag="VFL49_05375"
     CDS             complement(1224..1631)
                     /locus_tag="VFL49_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911438.1"
                     /translation="MSDMRMSTGTIVRISETTRDQIAFGLWLAMLAVALFLIAEGQSI
                     SMTAGLMLELTAAYSTFVLCGKSARSPFIHVVPYAFALAGTAFLCLAPDFPNAVQAGL
                     VFLAVTALMHGSVVFGTRKISADAEDPIYASAA"
     gene            1898..2512
                     /locus_tag="VFL49_05380"
     CDS             1898..2512
                     /locus_tag="VFL49_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911439.1"
                     /translation="MAGKLSHRRWWRPLQALAIVLLLAIPAGADAAPGTSAAGSTAHV
                     YLLRGVLNIFSLGMDQIAAKLEQRGIQTTVTNYLFWSSVADRAAADYRSGKVRTIILV
                     GHSSGATVLPDMVDRLTRLGAPVKLAIGLDSVFQTTLSGKVGRYINFYIGNGAGTRVA
                     RGKHLQGQLSNVDVENIPGGVGHITIDKNEIIQQKVISAILSSL"
     gene            complement(2540..3166)
                     /locus_tag="VFL49_05385"
     CDS             complement(2540..3166)
                     /locus_tag="VFL49_05385"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7911440.1"
                     /translation="MPLSLSQLFTSVWSGWLATWPTELLALIWIAWLVSWTVGSFWSA
                     RTEKRPVTWRARIHRFFVIIGGVLLTPWIAQVLGERPLWNAGNGGTYVLAVVVVAGLL
                     FTWWARIHLGRFWSNEITRKEGHRVIDTGPYGLVRHPIYTGLIAAILATGAAVGTVTA
                     ILGAVLISLGLSVKARAEENFLTSELDPSAYDSYRRQVPMLIPFLPHR"
     gene            complement(3156..4139)
                     /locus_tag="VFL49_05390"
     CDS             complement(3156..4139)
                     /locus_tag="VFL49_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7911441.1"
                     /translation="MTCVLVTGGSGFIGQHLVATLVASGRQTRVLDLRPPGRAVATAQ
                     YIKGSVLDHGLVDQTLDGVDEVYHLAGLPGMWAARKDDFHTVNCRGTEVVISAARKRG
                     VARLLHCSTESILFRSSPGGKAGVEDALLTADDMPGPYTRSKLLAERLAMEAAASGFP
                     VVIGSPTMPIGPHDHNLTPPTAMLRHFLGKRFQFYLDFVVNLVDVRDVATGLILAMER
                     GKVGQRYILGGESIPLQKLLEYVAAISGRDSLRVPVPGRLAEVTAAILEFMADHVTRT
                     PPSATAEAVRIALRSTALSTERAQRELGYAPRPIESALRETIAYLADTHAA"
     gene            complement(4136..4681)
                     /locus_tag="VFL49_05395"
     CDS             complement(4136..4681)
                     /locus_tag="VFL49_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HET7911442.1"
                     /translation="MSAFLRRQLTDYVEYHRDPMNCALHVAGIVILFLGAILPLSLWP
                     VTVFGLHANLGVLLAVPVLIYWIVLDVPIGLAIACAAALLLFTGAVIASHATVTAVWI
                     IFAVLIVIGVALQIIGHQVFERRQPALIDNPTHLLLGPVFVMAKLFISLGFRDDLAAI
                     IQQGPNSVYQHQSQGGAQQDR"
     gene            complement(4856..>5561)
                     /locus_tag="VFL49_05400"
     CDS             complement(4856..>5561)
                     /locus_tag="VFL49_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911443.1"
                     /translation="LGVEEKFYLAAPILVWVCLSQRRLWQKMTVVLALATLSIMLRAV
                     VFARMPGTINYDMFFAFLRSPFHLCLESLAAGVAVALFHADRNQPSDRKLGTGLMAGA
                     FIALLLWLPSEDFMSSIGWRDVVIQPILLAGLFALFVNGAVLMAKVALPFEPFVRAAA
                     RLSYTLYLVHIPLIPLSLSTAGGGNGSLGRFWIAYILLSICAALVLHYFVEKPFLLLK
                     ARIKSSSVAGRAVAPA"
BASE COUNT         1220 a         1607 c         1713 g         1021 t
ORIGIN      
        1 taccgtgccc tgcaccaagg tcacgggccg ttggctgttt ccgaaagccg agcttgatca
       61 ttggctcgtt tccagcgtct cgaggcccga aggcatggtg cggccggacg cagcacccat
      121 cgtcagcggc agccacgatc cgctgttcga atgggctttg cgggaaagcg gctcgggact
      181 ggcaatgctg gcggttggca gcgaggccgg attttcccgt ttcaccaccg gtgaaacgat
      241 tgccgcggca attcatctgc acgcgctcga agatctcgaa gcggacgcca acgttgccgc
      301 tttgcgaggc cgcaacgagc tgcaggacgc ggtgatgatc gcgttctgcc gccgcgagca
      361 gggattcgtg gtggcgccgg gaaatccgtt gaagctcgcc tcgatcgagg acatccgcaa
      421 gaagcgtgcg cgcatcgcca tgcggccgag aggcgccggt gcccagttgc tgctgttggc
      481 attattgcat cgcgctgaca tagcattcga ccagcttgtc tccgcggcgc cggtctgccc
      541 gaccggtccc gacattgcac aggcgatccg tgccggccgc gccgacaccg gcatcgctac
      601 gcgcggtgtt gccaacgcag ctgggctcga cttcaccccg attgtctggg agcgattcga
      661 tctggtgatg cgccagcgcg attatttccg gaccccgctg caatcgctgc tgaagttttt
      721 ccattcgccg gatttcgccg cgcgcgcgcg agaacttggc ggctacgatc tcaacactgc
      781 aggcaccgta cggctcgtaa actgatgacc gatgcgcaag tctggccgac cgaattgcgt
      841 ttgcacaagg accgcaaggc tttgaccgtc actttcgaca gcggggagcg cttcgaattg
      901 ccggccgaat acctgcgggt gaaaagtccg agcgcggaag tgcagggcca ttcggaagag
      961 gagcgaaaga ccgttcccgg caagcgaaat gtcgagattc ttgaggtatt gccgatcggg
     1021 aattacgccg tgcgtctcgt ttttgacgac atgcattcca ccggtatttt cggctgggat
     1081 tatctacatg acctcggccg caaccacgcc gcatactggc aggactatct cgacgagctt
     1141 gctgccagga acatgacgcg cgatccaccg gcgcgccgca gttagcagcg gcgctgtcct
     1201 ttcaacgagt ttggaacggg acgtcaggcc gcactggcgt aaatcgggtc ttctgcatcg
     1261 gcggagattt ttcgcgtgcc gaatacgaca gatccgtgca tcagcgcggt gaccgccagg
     1321 aacacgaggc cggcctggac ggcattcggg aaatcggggg cgagacagag gaaagctgtg
     1381 ccagcgagtg cgaatgcgta aggcacgacg tggatgaacg gactgcgagc cgatttgccg
     1441 cataagacga acgtcgaata ggccgcggtc aactcgagca taagccccgc ggtcatcgaa
     1501 atcgattgac cttcggcgat gagaaaaagc gcgactgcca gcatggcaag ccagagtccg
     1561 aaagcgatct gatcacgcgt cgtttcgctg atccgcacaa tcgtgccggt ggacatcctc
     1621 atatcgctca taacaatact cctggccaat ggacgcgctt caataggcga ggaggtcttt
     1681 cgcgatcggc actataggtg ccgtaatccg tgccacttga gtaagcagat tttaacgcgc
     1741 tgcaaaccgc cgtaatccgc ttggaatcca ggttggtctg gctaagatta ggctatgtga
     1801 cgtcgctatt ttaccgcgac ggatcaagga aacaccgggg tcgccgcgta cgcgagaggg
     1861 gggaatgata atgacgccag ttggtggctg ttttcgattg gccggcaaat tgtcgcatcg
     1921 gcggtggtgg cggccgctac aggcgctggc aatcgtgctc ctgcttgcca ttcccgccgg
     1981 cgccgatgcg gcaccgggaa cttccgccgc cggatcaacc gctcacgttt atctgctcag
     2041 gggcgtgctc aacatatttt cgttgggcat ggaccaaatt gcggccaagc tcgaacagcg
     2101 gggcattcag accactgtga ccaactatct cttttggtca tcggtggcag accgagctgc
     2161 cgcggattac agaagtggaa aagtgcggac catcattttg gtcggccact cttcgggggc
     2221 gacggttctg ccggatatgg tcgatcgcct gaccaggctc ggcgccccgg tcaaactggc
     2281 catcggtctg gattccgttt ttcagacgac gctgtcgggc aaggttggcc gctatatcaa
     2341 tttctatatc ggcaatggcg caggcacgcg cgttgcgcgg ggcaagcatc tgcaggggca
     2401 gctgtccaat gtcgatgtcg agaatatacc tggcggtgtc ggtcacataa cgatcgacaa
     2461 gaacgagatc atacagcaga aagtcataag cgcgatcctc tcctcacttt agatcgctga
     2521 cctgacaccg agaccgcagc taccggtggg gcaggaaggg tatcagcatc ggaacctgcc
     2581 gccggtagga atcgtatgcg ctcgggtcga gctccgaggt aaggaagttt tcttccgcgc
     2641 gcgccttcac cgacaagccc aaagaaatca gcaccgcgcc aagaatggcg gtcaccgtcc
     2701 cgacggctgc gcccgtcgcc agtatcgcgg cgatgagccc ggtatagatc ggatgacgca
     2761 cgagcccgta cggaccggtg tcgatgaccc tgtgaccttc ttttcgcgtg atttcattcg
     2821 accagaagcg tccgagatga attcgtgccc accaggtgaa caagagcccg gccacgacga
     2881 ccactgccag cacataggta ccgccattgc cagcgttcca cagggggcgt tcgcccagga
     2941 cctgtgcgat ccacggggtg aggaggactc ctccaatgat gacgaagaac cggtgaatgc
     3001 gtgcgcgcca ggtcacaggc cgcttttctg tgcgagccga ccagaaagaa ccaacggtcc
     3061 agctgacaag ccaggcaatc cagatgaggg caagcaattc ggtcggccag gtggcaagcc
     3121 acccgctcca gaccgaggta aacaactgac tgaggctaag cggcatgggt gtcagcgagg
     3181 taggctatgg tttcgcgcag ggcggattca atgggccgtg gcgcgtagcc gagctcccgc
     3241 tgcgcccttt cagtcgaaag tgccgtggat cgcaatgcga tacggacggc ctctgccgtg
     3301 gcggaaggcg gggtgcgcgt tacgtgatcg gccatgaact ccagtatagc agcagtcact
     3361 tctgcaagcc gccccggaac tggaacgcgc aggctgtcgc ggccgctgat cgcggcaaca
     3421 tattccagaa gcttttggag tggaatgctt tcgccgccga ggatgtaccg ctggccgacc
     3481 tttccgcgtt ccatggcaag gatcaggccc gtagcgacgt cgcgcacgtc gacgagattc
     3541 acgacgaaat cgagatagaa ctgaaaccgc ttgccgagaa aatgccggag catcgccgtt
     3601 ggcggagtaa ggttatggtc gtgaggcccg atcggcattg tcgggctgcc gatgaccacc
     3661 gggaaaccgg atgcggcggc ttccattgcg agtctctcgg cgagcaattt cgaccgggtg
     3721 tacgggcccg gcatgtcgtc cgcagtcagc aaggcatctt ccacgcccgc ctttcctccg
     3781 ggcgaggaac gaaaaaggat ggactcggtc gagcaatgga gtaaccgcgc aacgccacgc
     3841 ttgcgcgcgg ccgaaatgac gacctccgta ccgcgacaat tgaccgtgtg aaaatcgtcc
     3901 ttgcgcgccg cccacatgcc gggcaggccg gcaaggtggt aaacctcatc gacaccgtct
     3961 agcgtctgat cgaccagccc gtggtccagt accgagccct tgatatattg cgcggttgcg
     4021 acggcgcggc ccggagggcg gagatcgaga acgcgcgtct gccgaccgct cgccacgagc
     4081 gttgccacca agtgctggcc aatgaaaccg ctgccgcccg tgacgagaac gcaggtcatc
     4141 gatcttgctg cgcgccgccc tggctttggt gctggtagac cgagttcggc ccttgttgaa
     4201 ttatcgccgc aagatcatct ctaaacccga gcgaaatgaa caattttgcc atcacgaaca
     4261 ccggacccag gagaaggtga gtggggttat caataagtgc cggctggcgg cgctcgaaca
     4321 cctgatgccc gataatctgc agcgccacgc cgatcacgat caatacagca aaaattatcc
     4381 aaacggccgt gacggtcgcg tggctcgcga ttacggcgcc ggtaaaaagc aggagagcgg
     4441 ccgcgcaggc gattgccagg ccaatcggga cgtcgagcac gatccaatag atcagcacgg
     4501 ggacggccag gagaacgccc aagttggcat ggaggccaaa taccgtgaca ggccacaggc
     4561 tgagcggaag aatcgcccca agaaacagga tcacgatgcc ggccacgtgc agcgcgcagt
     4621 tcatggggtc ccggtggtat tccacgtagt ctgtaagctg acgccgaaga aaagcactca
     4681 tattgcgctc gctcccctgg accgcgcgac attgaggacg gaggatgaca tgaacttcgc
     4741 cgtccaaaca atgccgaaca acagatcctt tctgtcgcgg tccggaagga tgctgaattt
     4801 gggctaattg gcgctgtaaa cgaagggcgc tttagggatt gaatctaatg atctctcagg
     4861 ctggggcaac agccctgcct gcgaccgagc tgctcttgat gcgcgctttc agaagcaaga
     4921 aaggcttctc gacgaagtag tgaagtacaa gtgcggcgca gatcgacaac aaaatatagg
     4981 cgatccagaa tcgtcccaag cttccgttgc cgccacccgc ggtcgaaagc gagagcggaa
     5041 taagcgggat gtgaacaagg tagagcgtgt agctcagccg tgctgccgca cgaacaaacg
     5101 gctcgaaggg aagggcaact tttgccatca gaacggcgcc gttgacaaaa agcgcaaaca
     5161 accccgccaa aagaatcggc tgtatgacta cgtcgcgcca gcctatggag gacatgaaat
     5221 cttcgctggg aagccataac agcagggcaa taaaggcgcc tgccatcaac cctgtgccca
     5281 gcttgcggtc ggacggctgg ttgcggtcgg cgtggaacag cgcgacagcg acaccggcag
     5341 ccagcgactc caggcagagg tggaacggac ttcgcagaaa agcgaagaac atgtcgtagt
     5401 ttatagtacc gggcattctg gcaaagacga ccgcccgtaa catgatcgag agggtcgcca
     5461 gtgccagcac taccgtcatt ttctgccaaa ggcggcgttg cgagaggcaa acccagacca
     5521 gaattggcgc tgcgaggtag aatttttctt caacgccgag a
//
LOCUS       DASSRL010000261         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1214346, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000261 DASSRL010000000
VERSION     DASSRL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214346"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFL49_05405"
     CDS             complement(<1..421)
                     /locus_tag="VFL49_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET7911444.1"
                     /translation="MSESTGRVADATGNWVDSLAPPYARPYLRLARLDRPIGSWLLLM
                     PCWWSVGLAGMHEGRFPSLWHIVLFFIGAFAMRGAGCTWNDLVDRDLDGRVERTRSRP
                     IPSGQVTVAQATLFMLGQAIIGFLVLIQFNRLTVVTGI"
     gene            complement(418..903)
                     /locus_tag="VFL49_05410"
     CDS             complement(418..903)
                     /locus_tag="VFL49_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911445.1"
                     /translation="MSLHRALVVAGVVALFAGGAKAQFGPAVPQQEPPCLKDFSKLRD
                     DAEKKASAIRAAGERKAQPREACQLFTAFVAAQSKMVKFATDNSVWCGIPNQILANLK
                     DGLAKTSEVRTKVCQAAAAPPRPSGPSLSDALSSPVPDSNNVKTGRGTFDTLTGNALG
                     K"
     gene            1076..1825
                     /locus_tag="VFL49_05415"
     CDS             1076..1825
                     /locus_tag="VFL49_05415"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7911446.1"
                     /translation="MPRYEFRMPRLFVDAPLTPGGQVSLDRSQANYLLNVLRLGQGDP
                     VLMFNGRDGEWRGNLSGTGKRGAAIRLETCLRPQPRPGDLHFLFAPLKHTRLDYVVQK
                     AVEMGAAHLQPVMTRHTQVARVNLDRMRANAIEAAEQCGILHVPTVGAVVELAAAVSN
                     ADRLLVFCDESAEVADPLAALTAARSRRFPVSVLIGPEGGFADEERSMLAKLPNVVRI
                     ALGPRILRADTAAVAALALVQAVLGDWSSAE"
     gene            1917..2372
                     /locus_tag="VFL49_05420"
     CDS             1917..2372
                     /locus_tag="VFL49_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911447.1"
                     /translation="MTTFSLCLRGLLAIALIAVFAVSPVRAAGWIEKGIYLIGPRYDG
                     VLPPCDAALGTIASRFAQKEGRFWNSNLQILGFDRVRETAFRPWAPETVPRRYCSAVA
                     QVSDGRNHTVHYSIIEDAGMIGMFWGVEWCVVGLDRNWAYNPACKMARP"
     gene            2351..3193
                     /locus_tag="VFL49_05425"
     CDS             2351..3193
                     /locus_tag="VFL49_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HET7911448.1"
                     /translation="MQDGPALKAARSISFYSFLFLDCSWSQGTPTMEPLSQINFRFVL
                     FRIRKLMMWRSSSHRSALIGAIAVAHFFFFNAETVAQVRQHAASGQFDFYVLSLSWSP
                     SYCEAAVDRSRGARDRQCSGRPFAFVVHGLWPQYERGFPSNCQVPAPRLARAIVDEML
                     DIMPSPRLVFHEWDRHGTCSGLSPKAYFDLVRAAWHAVKIPQDYVQVDQPISVTPGEV
                     ANAFMKANPRLARDGINVSCDGRRLTEIRICLTKALTFRGCPEMARYSCRRDKVVMPA
                     VRGR"
BASE COUNT          584 a         1014 c          984 g          682 t
ORIGIN      
        1 cgatgcccgt gactaccgtt aaccggttga actggatgag caccaagaag ccgatgatcg
       61 cctggccaag catgaacagc gtggcttgcg cgacggtgac ttgcccggag ggaatcggcc
      121 gcgagcgcgt gcgctcaacg cgcccatcaa gatcgcgatc aaccagatca ttccaggtgc
      181 agccggcgcc acgcatggca aaggcgccga tgaagaacag cacgatatgc cacaaactcg
      241 ggaagcggcc ctcgtgcatt ccggccaggc cgactgacca ccagcacggc atcaacagca
      301 gccacgatcc gatcgggcgg tcgagtcggg caagccgcag atagggccgg gcatacggcg
      361 gagcgagact gtcgacccag tttccggtcg catcggcgac gcgtccggtg ctctcgctca
      421 ttttccgagt gcgttgcctg tcaaagtgtc aaaagtgcct cggccggtct tgacattgtt
      481 tgagtccggc accgggctgg acagcgcatc gctcaggctt gggcccgacg gacgcggcgg
      541 cgcggcggca gcctgacaga ccttggtgcg gacttccgaa gtcttggcga ggccgtcctt
      601 gaggttggcc aggatctggt tcgggattcc gcaccacacg ctgttgtcgg ttgcgaattt
      661 gaccatcttt gactgcgctg caacgaacgc agtgaacagt tgacaggctt cccgcggctg
      721 cgccttgcgc tctccggcgg cccggattgc gctggctttc ttctcggcat catcgcgaag
      781 cttggagaag tccttcaggc aaggaggctc ctgctgcggc accgcaggcc cgaattgcgc
      841 cttagcgcct cccgcgaaaa gcgcaaccac acccgccacg accaaggccc ggtgcaaact
      901 cattaagcct gatccctctc tgtatccggc ccactataac agtttcggag gccgtaccgt
      961 cctggcttat aacaacccga atgcgtcggg aaggcggcgg aatggcctcg ccgagcctcg
     1021 cgtagtcatg ccagaacgcg taaaacccgg ccatcgtctt gcgacaatta accccatgcc
     1081 gcgctacgaa tttcgcatgc cccggctttt tgtcgatgcg ccgctgaccc ccggcggcca
     1141 ggtctcgctt gatcgcagcc aggcaaatta tctgctcaat gtcctgcgcc tggggcaggg
     1201 cgatccggtt ctgatgttca acggacgcga tggcgaatgg cgcggcaatc tttcgggaac
     1261 cggaaaacgg ggagccgcca tcagacttga aacctgtctg cgcccacaac cacggcctgg
     1321 cgatctgcat ttcttgttcg ctccgctgaa gcacacgcgg ctcgactacg tcgtgcaaaa
     1381 ggcggtggag atgggcgctg cccatctgca acccgtcatg acgcggcaca ctcaggttgc
     1441 gcgcgtcaat ctcgatcgca tgcgcgccaa cgcgatcgaa gccgccgagc aatgcgggat
     1501 cctgcacgtg cccacggtcg gtgccgtggt cgaacttgca gcagccgtca gcaacgcaga
     1561 ccggctgctg gttttttgcg acgagagtgc cgaagtcgcg gatccgctcg ccgcgctgac
     1621 ggcggcacgg tctcggcgtt tccccgtttc cgtcctgatc ggtcccgagg gcggcttcgc
     1681 cgacgaagaa agatcgatgc tcgcaaagct cccgaatgtg gttcggatcg cgcttggtcc
     1741 gcgcattctg cgcgcggaca cggcggcggt ggcggcgctt gcgctggtcc aggccgttct
     1801 cggtgactgg tcaagcgcgg aatagcggtc tgtccggctt caaacctgtg tacccaagcg
     1861 tgggcgacct gctaaaatcg tgctagagtc taaattcggg tctggggagc tcaacgatga
     1921 cgactttttc gctctgcctc cgcggtcttt tggccatcgc actcatagcg gtctttgccg
     1981 tatcgccggt ccgcgccgct ggttggatcg aaaagggcat ctatctcatc ggcccccgat
     2041 acgatggcgt gcttccacca tgcgacgccg cgctcggcac aattgcctcg cgttttgctc
     2101 agaaggaagg gcgcttctgg aattccaacc tgcagatttt aggcttcgac cgcgtgcgcg
     2161 agactgcatt ccgtccttgg gcgccggaga cagtcccacg ccggtattgc tccgcggtcg
     2221 cgcaggtcag tgacgggcgc aaccacacgg tgcactactc gatcatagaa gacgccggaa
     2281 tgatcggtat gttctggggc gttgagtggt gcgtggtcgg tctggaccgc aactgggcct
     2341 ataatccggc ttgcaagatg gcccggccct gaaggctgcc cggtcgatct ctttttattc
     2401 ttttttgttc ttggactgtt catggtccca aggtacccct accatggaac cgttgagcca
     2461 aatcaatttc cggtttgttc tttttcgcat acgtaagctg atgatgtggc gttcttccag
     2521 ccatcgatcg gcactcatcg gggcgatcgc ggtcgcccat tttttctttt tcaatgccga
     2581 aactgtcgcg caggttcgcc agcacgccgc gtcggggcaa tttgattttt acgtcctgtc
     2641 actgtcgtgg tcgccttcat attgtgaggc ggcggtcgat cgttcgcggg gcgcacgcga
     2701 tcgccaatgt tcgggccggc cgttcgcctt tgttgtccac ggtctctggc cgcaatacga
     2761 acggggcttt ccatccaact gccaggtgcc agcgccgcgg cttgcgcgtg caatcgtgga
     2821 tgagatgctc gacatcatgc ccagtccccg actcgtcttc catgaatggg accggcacgg
     2881 gacctgctcc ggcctttcac caaaggccta cttcgatctc gtccgcgcgg cgtggcatgc
     2941 ggtgaagatc ccgcaggact atgtccaagt ggatcagccg atcagcgtga cgcccggcga
     3001 ggttgcgaat gccttcatga aagcgaaccc ccggcttgcg cgggatggca tcaacgtttc
     3061 atgcgacggc aggaggctga ccgaaatacg catctgcctg accaaggcgt tgacctttcg
     3121 cggctgtcct gaaatggcgc gctattcgtg ccgccgcgat aaagtcgtga tgccggccgt
     3181 gcgcggtcga taattcggtc ctatcgtgaa ctatcgccac gaattccatg ccggcaattt
     3241 tgccgacgtt ttcaagcacg ccgt
//
LOCUS       DASSRL010000262         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_76990, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000262 DASSRL010000000
VERSION     DASSRL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76990"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..942
                     /locus_tag="VFL49_05430"
                     /pseudo
     CDS             313..942
                     /locus_tag="VFL49_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            1083..1430
                     /locus_tag="VFL49_05435"
     CDS             1083..1430
                     /locus_tag="VFL49_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911449.1"
                     /translation="MKATLRTSTIGLLALLALGGAAQASDAGVTIRVSDYRFIGDDRF
                     SGGDHFDRPRHHEYDRRDWRRSYGRPGPHWHHARPVFGKPGWDRFDDCRVIVRRHINR
                     WGEHVVRRMRVCD"
     gene            1949..2158
                     /locus_tag="VFL49_05440"
     CDS             1949..2158
                     /locus_tag="VFL49_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7911450.1"
                     /translation="MNTGTVKFYNETKGYGFIQPDNGGKDVFVHATALERAGLRGLVE
                     GQKVSFDTAEDRRTGKIAVNNIQTA"
BASE COUNT          440 a          756 c          690 g          453 t
ORIGIN      
        1 ccgtagctcg gcagaatgtt aacttgattt gcccaaactg cccgtgtcgg tttaaagatc
       61 gacattcgtt tgacgacaga acacaatttt cggggcttgg gggcaatgaa ctgcgcaact
      121 tctgcggcgg tcgcatcctg cgcaattttt tcgatctttc tcgtatcaac ggcttgtgca
      181 gagagtagcg ggagtgccgc tgaatttccg aagctgcccc ttcctccaca aatccggccg
      241 cttaccgagc aagcgtttct tgcagccgat ggcctcgctc gcctccccgc ctctcgtgca
      301 acctatctgc cgatcgtcac acgcgaggcc accgcgcgtg gtcttcctcc ggccatcgcc
      361 gatgcggtca tgagcgtcga gagcgcctat aacccggcgg cgatcggagg tgtcggcgaa
      421 atcggcctca tgcaggtcag gccgagcacg gcggcaatgc tcggcttcga gggatcggac
      481 gcggagttgg cgcagcccga gaacaatatc cgttacggcg tcacctatct ggcccgggcc
      541 tggcgcctgg ccggtgggga tctgtgccgt gccctgatga aatatcgagc cgggcacggc
      601 gaggagcgca tgagcccgct cagcgtcgaa tattgccgac gtgtccgggt gcatcttgct
      661 tcgctcggct acggggccga tcatccgatc ctggcggctc ccatgccgga accggacttc
      721 attccggcgc ccgacgccgc aaaggcacct cgcatcgtgt catcgcgacc catgtcccgc
      781 accgccggct tcatcgcggt tcagcgggcg cggattgcag agacccgcac gcgactttca
      841 gccaagacga gggcccgcgc cttctgggct gctcacgagg cccggatggc cgtgctgcgg
      901 cgcaagctgg cgtccacgga cctcaccatc atcgcgcgct gaggcgagtt cggtcccggc
      961 gtgaagcggt gcattccgcc tgaacatccg ctgaaggagt cgttcggtcc agcttcaagg
     1021 gtgatgccgc acattgaacc catcgccgtc ctcacggtga ggacggcacc gggatcattc
     1081 cgatgaaagc cacccttcgg acttcgacga tcggcctgct ggcgctcctc gccctgggcg
     1141 gtgctgcaca ggcttccgac gctggcgtca cgatcagagt cagcgactat cgctttatcg
     1201 gcgacgaccg cttctccggc ggcgaccatt tcgaccgtcc gcgccatcat gaatacgacc
     1261 gccgggactg gcgccgctcc tatgggcgcc cgggacctca ttggcatcac gcccgacccg
     1321 tcttcggaaa gcccggctgg gaccgcttcg acgattgccg cgtgatcgtg aggcgtcaca
     1381 tcaaccgctg gggcgaacac gtcgtccgcc gtatgcgggt ttgtgattga cctgagggca
     1441 gccagacgaa cctccgacac cacatcgtca tggcaggccg caggcgaagc cgtccagcct
     1501 tcatccggtg agcaactgga tggcttcgct ctgctcgcca tgacgaagag ggatgccgcg
     1561 catcccgcgg caaggcctga ttccgtcaat gatgacgacg caatcgacct gccagagatg
     1621 aagtcaagta tgcaggatcg cacgatgcca ttcagatcag cactgtataa gtcctgcaaa
     1681 aattgacatc ttgatcacgg caagggttgt ctttttcctg ctgccggcat tcagcacggg
     1741 gacattgatc cctcggcagg cttgccaaat ggcctcccgg cacctaaata tacgttcatc
     1801 gactttggcc ggacgatcgg gccgttacgc cttggcacag ccaggcgcac gttctggact
     1861 ttctttccaa atttcgacga accgagtgtg agtttgcatc gaagcatctt gcactcacgt
     1921 gcacccaagc agtgataagg cctctgccat gaatactggc acagtgaaat tctacaacga
     1981 aaccaagggt tacggtttca ttcagccgga caacggcggc aaggacgtgt tcgtccatgc
     2041 gacggcgctc gagcgcgctg gtctgcgcgg cctcgtcgag ggccagaagg tatccttcga
     2101 tacagcggaa gaccgccgca ccggcaagat cgccgtcaac aacatccaga ccgcgtaagg
     2161 gatggattcc gccgtgcagc atgctgcgcg gcccacgaag ccgccctcct cagggcggct
     2221 tttctttcac gcatcgctcc ccggaataga tgagagatgc gtccaccttg agagcccgga
     2281 ctgcgatcct atccggtgct ttcacgcgct ccggccccgg ggcgcccaaa cgagaggac
//
LOCUS       DASSRL010000263         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_689551, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000263 DASSRL010000000
VERSION     DASSRL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689551"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFL49_05445"
     CDS             <1..392
                     /locus_tag="VFL49_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911451.1"
                     /translation="PDRSAWQKPTTTKPKTHETAKDPEYQPKPAARESLLNIPNLSNS
                     WAPHTRRLDLIRSKANAQYSDAGWAIQALQERIAHRSNSRFLLLFPEPTKNQRLRSTA
                     RVLVESARGVKRKTQQSYPHPATSTGR"
     gene            complement(591..1046)
                     /locus_tag="VFL49_05450"
     CDS             complement(591..1046)
                     /locus_tag="VFL49_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HET7911452.1"
                     /translation="MTVHLIKLCVGVSDVSQLLQEHERLAKQALKRKKPVEDHHWTRM
                     RPRRSEEVLDGGSLFWVVRGIVRVRHRILRLDDVRDADGQEYCAIIYDPKVILVEPRP
                     RRAFQGWRYLEAEDAPPDLPNQRQALANAAAEGDMPPQMLAELRTLGLF"
     gene            1126..1653
                     /locus_tag="VFL49_05455"
     CDS             1126..1653
                     /locus_tag="VFL49_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911453.1"
                     /translation="MSRLLAIILIGALAAGCTPGRIDRIRVEDGYDPVQYQFVPYTRA
                     LHADVSGNPFGMDQEAFNNLVSDTIQPPGVLASDGSPYRVHLAFNGSTSVNRDLACRA
                     SGGGASHGDMTLVAALCPGNGPALTFLTGSVSDIAGPDDPRFHQFVRSSVVKLFPGPT
                     IDNRNQNDRCFLPMC"
     gene            1859..3361
                     /locus_tag="VFL49_05460"
     CDS             1859..3361
                     /locus_tag="VFL49_05460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7911454.1"
                     /translation="MTFENLGLSPSILQAIADLGYQQPTPIQAQAIPVVLMGRDVLGC
                     AQTGTGKTAGFTLPMIEILSSGRAKARMPRSLILEPTRELAAQVADSFEKYGKNANLS
                     YALLIGGESFADQEKKLDRGVDVLIATPGRLLDLFERGKILMNDVKILVIDEADRMLD
                     MGFIPDVERIVGLLPKLRQTLFFSATMAPEIRKLADAFLMNPKEISVAPPATAASTVV
                     QGLAVVETEMKREGLRRLLRTENVTNALVFCNRKRDVEILFKSLSKHGFDAVRLHGDM
                     SQPARTETLDKFRRGEARLMVCSDVAARGLDIKGLSHVFNFDVPMHSEDYVHRIGRTG
                     RAGREGHAFTLAAPIDGKYVAAIEKLLGSPIPRIAIEGLEGAALSSGREHRRGGRHDR
                     ARQQQEKRQRKAAEVIDAVVPHPEIEGGRHAMLETSVALPQLEPEQHANRHHRRAGGR
                     DQEAQQHSNGRERRGPRRDYRGQDEGLPGVAFGDHTPAFLLRRGRSSEAR"
BASE COUNT          676 a         1155 c         1024 g          574 t
ORIGIN      
        1 acccagatcg ctccgcatgg cagaagccaa caacaaccaa acccaagacc cacgaaacag
       61 caaaagaccc ggaataccaa ccaaagcccg ccgcccgcga atcccttctc aatataccaa
      121 acctgtcaaa tagctgggcg ccgcacacgc gacgcctcga cctgattcgg tcaaaggcaa
      181 acgcgcaata tagcgatgcc ggatgggcga ttcaagcgct ccaagagcgc atcgcccaca
      241 gatccaacag ccgatttttg ctgctgtttc cggagccgac caagaaccag cggctccgtt
      301 cgacggcgcg ggttctagtc gagtccgcgc ggggtgtaaa gcggaaaacg caacaatctt
      361 atccacaccc cgcgacatcg acagggcgtt gaacaactgc ctgcagcaag cccggacctc
      421 tccctgccga ccaatcaccc ggagtgcgcg gcgttacgcg cccttgcagc atcacggaac
      481 cgcgcggcgc gcacgaggcc acgatcggcg ctttggcttg caaacgtgcc gctctgccag
      541 accgcaagca gcgatgtgtc ttccggtcaa tctgcacgca gcggagccgc ctagaacaga
      601 ccgagcgtgc gcaactcggc gagcatctgc ggcggcatat ccccttccgc ggccgcattg
      661 gcaagcgcct gacgctgatt gggcagatcc ggcggcgcat cctcggcctc caagtaccgc
      721 cagccttgga aagcgcggcg cggccgcggc tcgaccaaaa tcaccttcgg gtcgtagatg
      781 atcgcgcaat attcctggcc gtcagcatcc cgcacatcat ccaggcgcag aattcgatgc
      841 cggacgcgga caatgccgcg gacaacccag aagagcgaac cgccatcgag aacctcttcg
      901 gaccgccttg gccgcatgcg cgtccaatgg tgatcctcga ccggcttctt ccgcttcagg
      961 gcctgcttgg ccaggcgctc gtgctcctgg agcagctgac tgacgtcgct gacgccgaca
     1021 cagagcttga taaggtgaac ggtcatgtct cgcgaaatcg tgatgattgg tttcggaagg
     1081 atcggtcgtc aaatagaatc gaacagattc cctgaggaga agacgatgtc caggcttctc
     1141 gcaatcattc tgatcggcgc tctggcggcc ggctgcacgc ccggcaggat cgatcgtatc
     1201 cgggtggagg atggctacga tccggtgcaa taccagttcg tgccctacac ccgcgccctg
     1261 catgccgatg tgtccggcaa tcctttcggc atggatcagg aagcgttcaa caacctggtt
     1321 tccgatacca tccagccgcc gggcgtcctg gcgagcgatg gcagccccta ccgggtgcac
     1381 ctggccttca acggctccac cagcgtcaat cgcgaccttg cctgccgggc ctccggcggc
     1441 ggagcctcgc acggcgacat gaccctcgtt gcggccttat gccctggcaa cgggccggcg
     1501 ctgacgttcc ttacgggatc ggtctccgat atcgcggggc ctgacgatcc gcgattccat
     1561 caattcgtcc ggtcttccgt cgtgaagctg ttccccggcc cgaccatcga caaccgcaac
     1621 cagaacgacc gctgcttcct gccgatgtgc tgattgccgc tgcgtcgcat tgcaaaagca
     1681 cgatcgcctc gccatatgat ctgtcgatca cggggtagct gcaaactacc ggccaaccgg
     1741 ttgaattgac aaagcttgcc ttatccctat agtgcgcgcg actttccggc agtgggatcg
     1801 ggctcccctg cccctcatcg cccttcggcg agccggcaaa tgcccgcacc ttgagagaat
     1861 gacgttcgaa aatctggggc tcagcccctc catcctgcaa gcgattgccg atctcggcta
     1921 tcagcagccc acccccatcc aagcccaggc aattccagtg gttcttatgg gccgcgacgt
     1981 tttgggctgc gcccagaccg gcaccggaaa aaccgccggc ttcacccttc ccatgatcga
     2041 gatcctgagc agcggccggg ccaaggcccg catgccgcgc tctctcatcc tggagccgac
     2101 tcgggaactg gccgcgcaag tagccgacag cttcgagaag tatggcaaga acgcaaacct
     2161 gagctacgcg ttgctcatag gtggcgaaag cttcgccgac caggagaaga agctcgatcg
     2221 cggcgttgac gtgctgatcg cgacgccggg gcgtctgctc gacctgttcg agcgtggcaa
     2281 gatcctgatg aacgacgtca agatcctggt catcgacgaa gccgatcgca tgctcgacat
     2341 ggggttcatc cccgacgtcg agcgaatcgt cgggctgctg ccgaagctgc ggcagaccct
     2401 cttcttctcc gccaccatgg cgccggagat ccgcaagctg gccgatgcct ttctgatgaa
     2461 tccgaaggag atcagcgtcg cgccgccggc gactgcagcc agcacggtgg tccaggggct
     2521 cgcggtggtc gagacggaga tgaagcgcga ggggctgcgt cgattgctgc gcaccgagaa
     2581 cgtgaccaac gccctcgtct tctgcaaccg caagcgcgac gtggagatcc tgttcaagtc
     2641 gctctccaag catggcttcg atgcggtccg cctgcatggc gacatgtcgc agccggcacg
     2701 caccgagacg ctggacaagt tcaggcgcgg cgaagcgcgc ctgatggtgt gcagcgatgt
     2761 cgccgcccgc ggcctcgaca tcaagggcct cagccacgtc ttcaatttcg acgtgccgat
     2821 gcattccgag gattacgtgc accgcatcgg ccgcaccggc cgcgccgggc gcgagggcca
     2881 cgccttcacc ttggccgcgc cgatcgatgg caagtatgtg gcggcgatcg agaagctgct
     2941 gggcagcccc attccgcgca tcgcgatcga ggggctcgag ggtgcggcct tgtcgtccgg
     3001 ccgggaacac cgcagaggcg gtcgccacga ccgcgcgcgc cagcagcagg aaaagcgcca
     3061 gcgcaaggct gccgaagtca tcgacgccgt ggtgccgcat cccgagattg agggtggccg
     3121 gcatgccatg ctggaaacca gcgtcgcgct gccccagctc gagcccgagc agcatgccaa
     3181 tcgccatcac cgccgtgccg gcggacgcga ccaggaggca cagcagcatt ccaatggtcg
     3241 tgagcgtcgc gggccccgac gcgactaccg cggccaggat gaaggcttgc cgggcgtcgc
     3301 cttcggcgat cacacgccgg ctttcctgct ccgccgcgga cgctccagcg aagcccgctg
     3361 agcgcaaaga agaagcgcgg gagaaatctc ccgcgccgag gtgttccgca agcttgaagc
     3421 gttctagtt
//
LOCUS       DASSRL010000264         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_24198, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000264 DASSRL010000000
VERSION     DASSRL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24198"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VFL49_05465"
     CDS             complement(<1..988)
                     /locus_tag="VFL49_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7911455.1"
                     /translation="MKIIAAVVHERSGPFSVEQLDLCDPRSDELLVEVVTSGMCATDL
                     HGRDAYYPTRFPKVFGHEGAGMVRAVGNAVTKFKPGDHVVMTYPWCGECPNCRSQRLS
                     YCLHAFDLKMNGTRADGSTLHSKDGKPVYSAFFQQSSFGNFTIANERFAVKVRNDAPI
                     EHVCALACGGQTGAGAVLNVMKPRACDSFVVFGVGAVGLSALMSAKIAGCDPIIAVDV
                     HEHRLALARTLGATHTISHTNRTDVVEEVRKLTGIGARYSLETSALPSVFREAVDCLM
                     PAGTCILLGSARKGTETSFEMPFLQNGRTVRGVIQGDSVPQEFIPLLVDHIVA"
     gene            complement(1003..1662)
                     /locus_tag="VFL49_05470"
     CDS             complement(1003..1662)
                     /locus_tag="VFL49_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET7911456.1"
                     /translation="MSSHQPVRVDVVSDVVCPWCFIGKRRLEKAIALNPDIPVEVRYR
                     PYFLNDWIPREGISREEYLTTKFGSPERYNGIAQRVRATAASEGLTYAMDKISRQPNT
                     LDAHRLIRWAEGIGKAAEMKQKLMDLYFAEGADLTNHAVLVQAAADIGLDAEDVREAL
                     ASDKDVVEVEREALSAKEAGIQGVPCFILGGKFAISGAQAPEDLAEAIARLSESADAA
                     E"
     gene            complement(1699..>2360)
                     /locus_tag="VFL49_05475"
     CDS             complement(1699..>2360)
                     /locus_tag="VFL49_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7911457.1"
                     /translation="FSPRMVEAFLTVLAQEPRFRDIPVAVICETPPEFADALPNIEHV
                     AADPARLVGRIIPLVRLRAFEARLKRMLKSLDTDGVFDPQSGLLTRDSFWHDLKIAIT
                     EAADRSMPLSVARFSFGGHLDSRTSLDAARLMSRLIRNIDFATRDEEGAILVAFTQTD
                     LRNTHVIVRRIATSLKRMILGPQGEEPPAANVTLATLKAGDTPDTLMMRVVGSRMVAA
                     E"
BASE COUNT          449 a          729 c          754 g          428 t
ORIGIN      
        1 cggcgacgat gtgatcgaca agcagcggga tgaactcctg cggcacgctg tcgccctgga
       61 tcacgccgcg cacggtgcgg ccgttctgca ggaacggcat ctcgaaagat gtttcggtgc
      121 ccttgcgcgc gctgccgagc aggatgcagg tgccggccgg catcaggcag tcgaccgcct
      181 cgcggaacac ggatggaagc gccgacgttt ccagcgaata gcgtgcacca atcccggtaa
      241 gcttgcgaac ctcctcgacg acatcggtgc ggttggtatg gctgatcgtg tgtgtcgcgc
      301 cgagcgttcg ggcgagagca agccgatgct catgcacgtc aaccgcgatg atgggatcgc
      361 agcccgctat cttcgccgac atcagagcgg agaggcccac ggcgccaacg ccgaagacga
      421 cgaaactgtc gcaagcgcgt ggcttcatca cattgagcac cgcaccggca ccggtctgcc
      481 cgccgcaggc gagcgcgcag acgtgctcga tcggcgcgtc gttgcggact ttcaccgcaa
      541 agcgctcgtt ggcgatcgtg aaattgccga acgaagattg ctggaagaac gcgctgtaga
      601 ccggtttgcc gtccttcgaa tgcaatgtcg agccgtcagc gcgggtacca ttcatcttca
      661 ggtcaaaggc gtgcaggcag tagctcagcc gctgcgacct gcaattcggg cactcgccgc
      721 accagggata ggtcatcacg acgtggtcgc ccggcttgaa cttcgtgacg gcattgccga
      781 ctgcgcgcac catgccggcg ccctcgtggc cgaatacctt gggaaacctg gtcggataat
      841 aggcatcgcg gccatgcagg tcggtggcgc acatgccgct ggtcaccacc tcgaccagca
      901 gttcgtcgct tcgcgggtcg cagagatcga gctgctcgac gctgaacggg ccggagcgct
      961 catgcaccac ggcggcgata atcttcataa gcagtcccga aactactcgg cagcgtcggc
     1021 cgattcggac aggcgcgcga tcgcctcggc gaggtcctcc ggcgcctgcg cgcctgagat
     1081 cgcaaatttt cctcccagga tgaagcaggg gacgccttga atgccggcct ccttggcgga
     1141 aagtgcctcg cgctcgacct cgacgacgtc cttgtcgctt gcgagcgcct cgcgcacgtc
     1201 ctcggcgtca aggccgatgt ctgcggcggc ctgcaccagc acggcgtgat tggtgagatc
     1261 ggcgccttcg gcaaaataaa ggtccatcaa tttctgcttc atctcggcgg ccttgccgat
     1321 cccttcggcc cagcggatca ggcgatgggc gtcgagcgta ttgggctggc ggctgatctt
     1381 gtccatggca taggtaagcc cttcggatgc cgccgtcgcg cgaacgcgtt gcgcgatgcc
     1441 gttgtagcgc tcgggagaac cgaacttggt ggtcagatac tcctcgcgcg aaatgccttc
     1501 gcgcgggatc cagtcgttga gaaagtaggg ccggtaacgc acttcgaccg ggatgtcggg
     1561 attgagggcg atcgccttct cgagccggcg cttgccgatg aagcaccatg ggcagaccac
     1621 gtcggaaaca acgtcgacac gaaccggttg atggctggac attggaggct ccctttgcgg
     1681 gtgagcctag gccgccaact attcggccgc aaccatgcgg ctgccgacaa cgcgcatcat
     1741 cagcgtatcg ggcgtatcgc cggccttcag cgttgccagc gtgacattcg ccgccggtgg
     1801 ttcctcgccc tgtgggccga ggatcatgcg cttcagcgag gttgcgatcc ggcggacaat
     1861 cacatgcgtg ttgcgcagat cggtctgggt aaaggcaacc aggatagcgc cttcctcgtc
     1921 acgcgttgcg aaatcgatat tgcggatcag ccgtgacatc aaccgtgcgg catcgagact
     1981 ggtacggctg tccaagtgcc ccccgaatga aaatcgcgcg accgagagcg gcatgctgcg
     2041 atcagccgct tcggtaattg cgatcttcaa gtcgtgccag aatgaatccc tcgtcagcag
     2101 tccgctttgc gggtcgaata cgccatcagt atcgagcgac ttgagcatgc gcttgagccg
     2161 cgcttcaaag gcgcgcaggc gaacaagcgg aataatccgt ccgacgagac gcgcagggtc
     2221 ggctgcgaca tgctcgatat tggggagggc atcggcaaat tccggaggcg tttcgcagat
     2281 gaccgcgacc gggatatcgc gaaaccgcgg ctcctgcgcc agcacggtca ggaatgcctc
     2341 caccatccgc ggagaaaagc
//
LOCUS       DASSRL010000265         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1284249, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000265 DASSRL010000000
VERSION     DASSRL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284249"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFL49_05480"
     CDS             <1..327
                     /locus_tag="VFL49_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET7911458.1"
                     /translation="GTLEKYGCFVGSHAVVILSDKDDIKAAALNLMRFFEDESCGQCT
                     PCRVGTEKAASLMAKGPWDVKLLEELSAAMRDASICGLGQAAPNPLLCVLKYFPDELT
                     KPLGSW"
     gene            330..>2514
                     /locus_tag="VFL49_05485"
     CDS             330..>2514
                     /locus_tag="VFL49_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7911459.1"
                     /translation="MATNGKAATVKFTLDGKEVSARADETIWQVAKRHGTDIPHLCYS
                     PEPGYRADGNCRACMVEIEGERVLAASCIRKPTEGMKVKAASDRAKTARQMVFELLVT
                     DQPERATSHDPQSKFWSWADRMGIDVGRFPEREKMPGPDRSHPAMAVNLDACIQCNLC
                     VRACREVQVNDVIGMAGRGHGEKIVFDFDDPMGQSTCVACGECVQACPTGALMPATLV
                     NDKNVRTEFPDREVHSVCPYCGVGCQITYYIKNDKLLYVSGRNGPANENRLCVKGRFG
                     FDYVHNPQRLTKPMIRKEGVPKVPHEVIDPMNPWTHFREATWEEALDKAAAGLKKIRD
                     RDGSKALAGFGSAKCSNEEAYLFQKLVRAGFGTNNVDHCTRLCHASSVAALLEGVGSG
                     AVSATFNECRNSDVIVVIGANPTENHPVAATFFKQAAKRGAKLIVMDPRGQALKRHAW
                     RMLQFKNGTDVAMLNAILNVIVTENLHDKQYVQTYVEGFGPFADSIKDFTPEEMAPIC
                     GIPADTLREVARTFARAESAIIFWGMGVSQHTHGTDNARCLIALSLITGQIGRPGTGL
                     HPLRGQNNVQGASDAGLIPMFFPDYKSVEDSAIRAKVENLWKTKLDPKKGLTVVEIMD
                     AVHADVIKGMYILGENPAMSDPDLNHAREALAHLEHLVVQDLFLTETAVYADVVLPAS
                     AWPEKDGTVTNTNRQVQMGRQALPLPGDAKADWWITQELARRMGLD"
BASE COUNT          502 a          783 c          801 g          428 t
ORIGIN      
        1 ggaacgcttg aaaaatacgg ctgcttcgtg gggtcgcacg cggttgtgat cctctccgac
       61 aaggacgaca taaaggctgc cgcgttgaat ctgatgcggt tcttcgagga tgaaagctgc
      121 ggccagtgca caccctgccg cgtcgggacc gagaaagcgg ccagtctgat ggccaagggc
      181 ccatgggacg tgaagctgct cgaagagctg tcggccgcca tgcgcgatgc gtccatttgc
      241 gggctcggcc aggcggcgcc gaatccgctg ttgtgcgtgc ttaaatattt tccggatgaa
      301 ctgaccaagc cattgggaag ctggtgatca tggctacgaa cggcaaagct gcgacggtga
      361 aattcacgct cgatggcaag gaagtctccg cgcgggcgga cgagactatc tggcaggttg
      421 ccaagcgcca cggcaccgac attcctcacc tgtgttattc gcccgagccc ggttatcgcg
      481 ccgacggcaa ttgccgcgcc tgtatggtcg agatcgaagg cgagcgcgtg ctcgcggcca
      541 gctgcatccg caagccgacg gaaggcatga aagtgaaggc ggcctccgat cgcgccaaaa
      601 ccgcgcggca gatggtcttt gagctgctgg tcaccgatca gcccgagcgc gcgacctcgc
      661 atgatccgca atcgaagttc tggagctggg ccgaccgcat gggcatcgac gtcggccgct
      721 tccccgagcg cgaaaaaatg ccggggcccg accgcagcca cccggcgatg gcggtcaacc
      781 tcgacgcctg catccagtgc aacctctgcg tccgcgcctg ccgcgaagtg caggtcaacg
      841 acgtcatcgg catggccggc cgcggccatg gcgagaaaat cgtcttcgac ttcgatgatc
      901 cgatggggca gtcgacgtgt gttgcctgcg gcgagtgcgt gcaggcctgc ccgaccggcg
      961 cgctgatgcc ggcgactttg gtcaacgaca agaacgttcg taccgaattt cccgaccgcg
     1021 aggtgcacag cgtgtgcccc tattgcggcg tcggctgcca gatcacgtac tacatcaaga
     1081 acgacaagct cctctacgtc agcggccgca acggaccggc gaacgagaac cgcctgtgcg
     1141 tgaagggccg cttcggcttc gactacgtgc acaatccgca gcgcctcacc aagccgatga
     1201 tccgcaagga aggcgtgccg aaggtgccgc acgaagttat cgaccccatg aacccgtgga
     1261 cgcatttccg cgaagcgaca tgggaagagg cgctcgacaa ggctgcggcg ggcctcaaaa
     1321 agatccgcga ccgtgatggc tcgaaggcgc tagccggctt cggatcggcg aagtgctcga
     1381 acgaagaggc atacctgttc cagaagctgg tgcgcgcggg attcggcacc aataacgtcg
     1441 accactgcac gcggctttgc cacgcgtcat cggttgcggc gctgcttgaa ggcgtcgggt
     1501 cgggcgcggt ctcggcaacg tttaacgagt gcaggaattc cgacgtgatc gtcgtcatcg
     1561 gcgccaaccc gacggaaaac catccggtcg cggcgacctt cttcaagcag gccgccaagc
     1621 gcggcgccaa gctgatcgtg atggatccgc gcggccaggc gctcaagcgt cacgcctggc
     1681 gcatgctgca attcaagaac ggtaccgacg ttgcgatgct caacgcgatc ctcaacgtca
     1741 tcgtgaccga gaacctgcac gacaagcagt acgtgcagac ctatgtcgaa ggcttcggcc
     1801 cgttcgcgga cagcatcaag gatttcacgc cggaagagat ggcgccgatc tgcggtattc
     1861 ccgccgatac gctgcgcgaa gtggcgcgta cctttgcgcg cgccgaaagc gccatcattt
     1921 tctggggcat gggcgtgtcg cagcacacgc acggcaccga caatgcgcgc tgcctgatcg
     1981 ccttgtccct gatcaccggc cagatcggcc ggcccggcac cgggttgcat ccgttgcgcg
     2041 ggcagaacaa cgtacagggc gcgtccgacg ccggcctcat cccgatgttc ttccccgact
     2101 acaagtcggt ggaggattcg gcgattcgcg ccaaggtcga gaatctttgg aagaccaagc
     2161 tcgatccgaa aaagggcctg accgtggtcg agatcatgga tgccgtccat gccgacgtga
     2221 tcaagggtat gtacatcctc ggcgaaaatc cggcgatgtc ggatccggac ttgaatcacg
     2281 cacgcgaggc gctggcgcat ctcgagcatc tggtcgtgca ggatctcttc ctgactgaga
     2341 cggccgtcta tgccgacgtg gtgctgccgg cttcggcctg gccggaaaaa gacggcaccg
     2401 tcaccaacac caaccggcag gtgcagatgg gccgtcaggc gctgccattg ccgggcgatg
     2461 ccaaggccga ctggtggatc acgcaggaac tcgcccgccg tatgggcctc gact
//
LOCUS       DASSRL010000266         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1092001, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000266 DASSRL010000000
VERSION     DASSRL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092001"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFL49_05490"
     CDS             <1..348
                     /locus_tag="VFL49_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911460.1"
                     /translation="VDSDPFERVIFFIVSFFATSFVGAVAILPAALVIAISEAARMRS
                     FIYYGVGGALVALASYYGSDISVQLENTTDVTPVANALQLAAAAGILGGLTYWLIAGR
                     NAGRWREQPTPSA"
     gene            409..1185
                     /locus_tag="VFL49_05495"
     CDS             409..1185
                     /locus_tag="VFL49_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7911461.1"
                     /translation="MNRTGLLIALAIAVVSGLAFGLYPGLDLDVARYFHAFEDSNHNM
                     FALRIYPPLMRARNIGLWVGTILVAPAVCALVIKLLLPRRKMLMPGRAVVFLIATMAL
                     APGLLVNVLLKDHWGRPRPIDVTLFGGNQNFVAWWDPRGDCPSNCSFVSGDVAGAVWT
                     IAPAALAPPHWRALAYAAALALSFGMAAIRVMAGAHFPSDVIFAGLFTFLIIWITYAV
                     IYRWRPTRFSDEDVEQTIERIAMPGYVFIVGLFNGSRKAK"
     gene            1267..>2336
                     /locus_tag="VFL49_05500"
     CDS             1267..>2336
                     /locus_tag="VFL49_05500"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7911462.1"
                     /translation="MADDKTRKPVKKVVLAYSGGLDTSIILKWLQTNYGCEVVTFTAD
                     LGQGEELAPAREKALLLGIKEENIFVEDLREEFVRDYVFPMFRANAVYEGQYLLGTSI
                     ARPLIAKKQIEIAEKVGADAVAHGATGKGNDQVRFELTYYALKPDVTVIAPWREWDLT
                     SRTKLIEFAEKHQIPIAKDKRGEAPFSVDANLLHTSSEGKVLEDPGQEVPDYVFSRTV
                     DPEKAPDEPTVITVDFEKGNPVAVNGKKLTPAALLKLLNDLGKANGIGRLDLVENRFV
                     GMKSRGMYETPGGTILLAAHRGIESITLDRGAAHLKDELMPKYAELLYYGFWFTPERE
                     MLQAAIDRSQELVTGRVRLKLY"
BASE COUNT          444 a          671 c          711 g          510 t
ORIGIN      
        1 gtcgactccg acccttttga gcgcgtgata ttcttcattg tttccttctt cgccaccagc
       61 ttcgtcggcg ccgttgccat attgccggcg gcgctggtga tcgcgatctc ggaggcggcg
      121 cgcatgcgca gcttcatcta ctacggcgtc ggcggtgctt tggtggctct cgcgtcttat
      181 tatgggtccg acatctcagt gcaactcgag aatacgaccg atgtgacgcc ggtggcgaat
      241 gccctgcaac tggcagctgc cgccgggatc cttggcggcc tgacttattg gctgatcgcc
      301 ggccgcaatg caggccgatg gcgcgaacag cctacaccct ctgcctgatc cgaccggacg
      361 attgcaatcg gctttctatt ttccggggcg tcggctagac gagccggcat gaaccgcacc
      421 ggattgctga tagcgctcgc cattgccgtt gtttccggac tggcattcgg gctttatccc
      481 ggcctcgacc tggatgtcgc gcgatatttc cacgcgttcg aggacagcaa tcacaatatg
      541 tttgcgttgc gcatctatcc gccgctgatg cgggcccgca atatcgggct ttgggtcggc
      601 accatcctgg tggcgcccgc ggtgtgtgct ctcgtcatca agctactgct gccacgtcga
      661 aaaatgttga tgccgggccg tgcggtcgta ttcctgatcg caaccatggc acttgccccg
      721 ggactgctgg tgaatgtcct gctcaaggat cattggggcc ggccacgacc catcgacgtg
      781 acgctgttcg ggggcaacca gaatttcgtc gcctggtggg atccgcgtgg cgattgtccg
      841 tccaattgtt cattcgtgtc cggggatgtg gcgggtgcgg tgtggacgat tgcgccggcc
      901 gcgctggcgc caccgcactg gcgcgcgctt gcctatgcag cggcgctcgc gctcagcttc
      961 ggcatggcag cgatccgggt gatggccggc gcacattttc cctcggatgt tatcttcgcc
     1021 gggttattca cctttttgat tatttggatc acttacgccg tcatctaccg ctggcgcccg
     1081 acgcgctttt cggatgaaga cgttgagcag acgatcgagc gcatagcgat gcccggctac
     1141 gtttttattg tcggattgtt caacggaagc cgaaaggcaa aatgaaatcg ttaacgagga
     1201 cgcgctttgc gacgctggcc tttggcccta tagtccgccg cgattttcca ctgcagcagt
     1261 tgtccaatgg ccgacgacaa gaccaggaag cctgttaaaa aagttgtgct cgcctattcc
     1321 ggcgggctcg atacttcaat catcctgaag tggctgcaga ccaactacgg gtgcgaggtg
     1381 gtgactttca ccgccgatct ggggcaaggc gaggaactcg cgcccgcacg cgagaaggca
     1441 ttgctgctcg gcatcaaaga agaaaacatt ttcgtcgagg acctgcgcga agaatttgtg
     1501 cgcgattatg tgttccctat gttccgggca aatgcggtgt acgagggaca gtatctgctg
     1561 gggacgtcca tcgcgcggcc cctgatcgcg aaaaagcaga tcgagatcgc cgaaaaggtt
     1621 ggcgctgatg ccgtcgcaca cggggccacc ggcaaaggca acgatcaggt gcgtttcgaa
     1681 ctgacttact acgcgctcaa gcccgacgtg acggttattg cgccgtggcg cgagtgggac
     1741 cttacctcgc gcaccaagtt gattgaattc gcggaaaagc accagattcc gatcgccaag
     1801 gacaagcgcg gcgaggcgcc gttctcggtc gacgccaacc ttctgcacac gtcgtcggaa
     1861 gggaaagttc tggaggatcc tgggcaggag gtgccggatt atgttttctc tcgcacagtg
     1921 gatccggaaa aggcgcctga cgagcccact gtgatcacgg tcgacttcga gaaaggcaat
     1981 ccggttgcgg ttaacggcaa aaaactgacg cctgccgcac tgctcaagct acttaacgat
     2041 cttggcaaag cgaacgggat tggacggctc gatcttgtcg agaatcgctt cgtcggcatg
     2101 aagtcgcgcg gcatgtacga gacaccgggc ggcaccatac tgctggccgc gcatcgtggt
     2161 atcgaatcga tcacgctcga ccgtggcgcg gcacacctca aggacgagtt gatgccgaaa
     2221 tacgcggaac tgctctacta cggtttctgg ttcacgcccg agcgggaaat gctgcaggct
     2281 gcgatcgaca gaagtcagga actggtgacc ggccgggtcc ggctcaagct ctataa
//
LOCUS       DASSRL010000267         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1476696, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000267 DASSRL010000000
VERSION     DASSRL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476696"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFL49_05505"
     CDS             complement(<1..1076)
                     /locus_tag="VFL49_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HET7911463.1"
                     /translation="MKPGPERAVARERSSFLAELRDRSPADPTGPRFLIEDIFPSVDG
                     GRYPVKRIAGESVDVWADIFREGHDVLAAALLWRPESDADWRRVPMQLHGNDRWHGSF
                     TPPAPGVYLFAIEAWTDQFATWRKEFGIRKDAGQDLSLAAREGLELVSQLRPKNRKAR
                     TIIDDYAQRFAAEGNPDLLLDDALAAAMERTETRPDLTRSQAIPVLAERERARFGAWY
                     EMVPRSQGTVPGRHGTFDDCIARVPEIATLGFDVIYLTPIHPVGRTNRKGKNNSLTAG
                     PDDPGSFYAIGNESGGHDAIEPQLGTIKDFRRLVDACHENDMEIALDFAVQCSLDHPW
                     LKEHPEWFHRRPDGTLKYAENPPK"
     gene            1298..2890
                     /gene="malQ"
                     /locus_tag="VFL49_05510"
     CDS             1298..2890
                     /gene="malQ"
                     /locus_tag="VFL49_05510"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7911464.1"
                     /translation="MLHAFQGDGRRYWLLAVQLYMLRSDRNWGHGDFGDLARIIPLAA
                     SRGAAGIGLNPLHALFIERAGEASPYAPNSRIFLNPLYIDVDAIPDFPGTTAAGIDLD
                     ALRTGDLIDHGQVARAKLDGLRLAFEHFLTSAQDGRRADFDAYRREQGERLRQFACFE
                     VLRARRAPARWQDWPAPWGNPSRQDLLRLREEERTQCEFHEYIQWIADRQLKQCKETA
                     RECGMSVGLYLDVAVGIHPDGADAWIAQDRVLSDVSIGAPPDEFTPSGQNWGLAPFNP
                     ATLPNDDFAQLRELMRSAMRHAGAIRLDHVLGLKRIFMIPHGSPASEGTYVRFPFEQS
                     LRVIAEESLKALCIVIGEDLGTVPEGFRETITRWGLWTYRVVLFEREEDGRFRPPEWY
                     PVEALATFNTHDLPSLRGWLEGHDLHTKRKIGLDPGESDEARAWARQCLDTMLSDRSF
                     SADGNQLAAIARFLAQTPSRLVVIALDDIVGALEQINIPGTFDEHPNWRWRLPVSLED
                     LERNENLKIVADVFARAGRGFG"
     gene            complement(2892..>4097)
                     /locus_tag="VFL49_05515"
     CDS             complement(2892..>4097)
                     /locus_tag="VFL49_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET7911465.1"
                     /translation="LVEEGADRRVRMAHLAFLGSHAVNGVSHLHTDLLRVTVFHDLAR
                     TTSTRIVNKTNGITFRRWLFEANRPLIELLISTLGDRVLDDPACLRELEQKVGDDQFV
                     QRYRHARLVNKSNLVAWLHGSVDIRVDAHALFDVHIKRIHEYKRQLLNILSTIALYDY
                     IRAHPHDAHVPRVKIFAGKAAASYERAKLIIKLVNDVASVVNDDPIVGDRLKVIFVPN
                     YNASLAERIIPAADLSEQISTAGMEASGTGNMKLALNGAITIGTLDGANIEIREHVGN
                     ENIVIFGMTAEQVADRNRAEFKGAQAVARSPRLAAVIEGLAGGRFSPDDPTRYEDLAQ
                     ALLGYDPFMVAADFDAYWNAQRSVDQLWQSPSKWWRASILNTARMAWFSSDRAIREYA
                     QDIWHIPAD"
BASE COUNT          805 a         1196 c         1233 g          863 t
ORIGIN      
        1 ttcttcggcg ggttctcggc gtacttcagc gtgccgtccg ggcggcggtg aaaccactcc
       61 ggatgctcct tcagccatgg atgatcgagt gagcactgca ccgcgaagtc cagtgcaatt
      121 tccatatcgt tttcgtgaca cgcatcgacc agtcgacgga aatcttttat cgttcccagt
      181 tgcggctcga ttgcatcgtg tccgccgctc tcgttgccga tcgcataaaa gctgcccgga
      241 tcatcgggtc ccgcggtcag ggaattattc ttgcctttgc ggttggttcg gccgaccgga
      301 tggatcgggg tgagatagat cacgtcaaag ccaagagttg cgatttccgg aacccgtgcg
      361 atgcagtcgt cgaacgtgcc gtgccggccg ggaacggtcc cctggcttcg cggcaccatt
      421 tcataccagg cgccgaaacg cgcacgctca cgttccgcca atacaggaat ggcctgactg
      481 cgcgtaaggt ccgggcgcgt ctcggttctt tccatggccg ccgcgagcgc atcgtcgagc
      541 agcaggtcag ggttaccctc ggccgcaaat ctctgcgcgt agtcgtcaat gatcgttcgc
      601 gccttgcggt tcttgggcct gagctgtgat accagctcaa gcccttcacg cgcggccaga
      661 gaaaggtcct ggccggcatc ctttctgatt ccgaactcct tgcgccaggt ggcgaactga
      721 tcggtccatg cctcgatggc aaagagatag acacccggag caggcggggt gaaactgcca
      781 tgccaccgat cgttgccgtg caactgcatc ggtacgcgcc gccagtcggc atcactctcc
      841 gggcgccaca ggagtgctgc cgcgaggaca tcgtgccctt cgcgaaaaat atctgcccag
      901 acgtcgacgc tttcgccggc aattcgtttt accgggtaac ggccgccatc aacggaagga
      961 aaaatgtctt cgatgaggaa acgcggccct gtcggatcag cgggagacct gtcccgcagc
     1021 tccgcgagga aactagatcg ctcgcgcgca acagcacgct cgggtccggg cttcattgtt
     1081 aaggtctcgg cttacccgaa acgaaaacca caggatttcc cgaaagttcc ttccgaaaat
     1141 gaatgacctg gccgcagaag cgcgaatttg gggcattgaa cccgggtatc acgacatttt
     1201 tgggcgctgg cacgcggcca gcgaggaaac ccaacggcga ctaatgagag cgttgtcacg
     1261 aggcaaaaag cggccgggcc tttctcctgc ggcggaaatg cttcatgcat tccagggaga
     1321 cgggcgccgg tactggctcc tggctgtcca gctttacatg cttcgctcgg accgaaattg
     1381 gggtcacggc gatttcggtg atctggcgcg catcattccg cttgcggcat cgcgcggcgc
     1441 ggcgggtatt ggcctgaacc cgctgcatgc gctgttcatc gagcgtgccg gcgaggctag
     1501 cccctacgct ccaaacagcc gcatcttcct caatcctctc tatatcgatg tcgatgcgat
     1561 tccggatttt ccgggcacta cggcggcggg cattgacctg gatgcacttc gcacaggcga
     1621 tctcatcgat cacgggcagg tcgcgcgtgc caaactcgac ggcctgcgtc ttgcctttga
     1681 gcattttctg acttcggcgc aggatggccg ccgcgcggac ttcgacgcct atcgccggga
     1741 acaaggtgag cggctccggc agttcgcctg tttcgaagtc ttgcgcgcga ggcgtgcacc
     1801 ggctcgatgg caggattggc ctgcgccttg ggggaatccg agccggcagg acttgcttcg
     1861 tctgcgggaa gaagagcgaa ctcaatgcga attccacgaa tacatacagt ggattgccga
     1921 tcgtcagctc aagcagtgca aagaaaccgc gcgtgaatgc ggtatgtctg tcgggctcta
     1981 tctcgatgtt gccgttggca ttcatcccga tggtgccgat gcatggatcg cgcaggaccg
     2041 cgtcctctcc gacgtcagca tcggcgcgcc acccgacgaa ttcactccga gcggacagaa
     2101 ctggggtctc gctcctttca atcccgcaac actgccgaat gacgatttcg cgcagttgcg
     2161 cgagctgatg agatcagcaa tgcggcacgc cggcgcaatc agactcgacc acgttctggg
     2221 gttgaaacgg attttcatga ttccgcacgg gtcgcctgcc tcagaaggga cctatgtccg
     2281 ctttcccttc gagcaatctc tgcgtgtgat cgccgaggaa agcctgaaag cgctttgcat
     2341 tgtcatcggc gaagatcttg gcacggtacc tgagggcttc cgcgaaacaa ttacgcgctg
     2401 gggtctttgg acttatcgcg tggtgctgtt cgagcgggaa gaggatggcc gtttccgccc
     2461 gccggaatgg tacccggtgg aggccctcgc taccttcaac acgcatgacc ttcccagtct
     2521 gcgtggatgg ctggaagggc atgacttgca cacgaagcgc aagatcgggc tcgatcctgg
     2581 agagagcgac gaggcgcgcg cctgggcccg gcaatgcctc gacacgatgc tgagcgaccg
     2641 gagtttttcc gccgatggca atcagctcgc ggcaattgcc cgttttcttg cacaaacgcc
     2701 ttcacgtctc gttgtgatcg ccctcgacga cattgtgggc gcgctggagc agatcaacat
     2761 tccaggcacc ttcgacgaac atccaaactg gcgctggcgg ttgcccgttt ctctcgagga
     2821 tcttgagcgg aatgagaact tgaaaattgt tgccgatgtg tttgcgcgag cgggccgggg
     2881 cttcggatag ttcagtctgc cgggatgtgc cagatgtcct gcgcatattc gcggatggcg
     2941 cggtccgagg aaaaccacgc catccgggcc gtattgagaa tcgatgcgcg ccaccatttg
     3001 gaaggcgact gccacaattg atcgacgctg cgttgagcgt tccaataggc atcgaaatcg
     3061 gccgccacca tgaagggatc atagccgagc agagcctgcg caaggtcttc gtagcgcgtg
     3121 ggatcgtcgg gagaaaagcg gccgcccgca agcccctcaa tcacagcggc aagtcgtggc
     3181 gaacgagcaa ccgcctgtgc gcccttgaat tccgcgcggt tcctgtcggc gacctgttcc
     3241 gccgtcatcc cgaaaatgac gatgttttcg ttcccgacat gctcgcgtat ttcaatattg
     3301 gccccgtcaa gcgtgccaat ggtgattgca ccattgagtg cgagcttcat gttgccggtg
     3361 ccggatgcct ccataccggc ggtggaaatc tgctcagaca gatcggcggc ggggatgatc
     3421 ctttcggcca ggctggcatt gtagttgggg acaaatatca ccttgagccg gtcgccgacg
     3481 atcggatcgt cattgacgac gctggcgacg tcattcacca gcttgataat cagcttggcg
     3541 cgctcgtagg acgcggccgc ctttcctgca aagatcttca cacgcggcac atgtgcgtcg
     3601 tgcgggtggg cgcggatgta atcgtagaga gcaatcgtcg agagaatatt gagaagctgc
     3661 cttttgtatt catgaattcg tttgatgtgg acgtcgaaca gggcatgcgc gtcaacacgg
     3721 atgtcgaccg agccatgcag ccaagcaacg aggttgcttt tattcacaag ccgtgcgtgc
     3781 ctgtaacgtt ggacaaattg gtcgtcgcca actttttgct cgagctcgcg caggcaggcc
     3841 gggtcatcga gcacacgatc gcccagcgta gaaatcaaca gttcgatcaa cggcctgttg
     3901 gcctcgaaga gccaacgccg gaaagtgatg ccattggtct tgttgacgat ccgtgtcgat
     3961 gttgtgcgcg caagatcatg gaataccgtg acacgcagca aatcggtatg aagatgtgaa
     4021 acgccattga ccgcgtggct tccgagaaaa gccaggtgtg ccatgcgcac gcgtctgtcc
     4081 gcgccctctt cgacgag
//
LOCUS       DASSRL010000268         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_671873, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000268 DASSRL010000000
VERSION     DASSRL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671873"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="VFL49_05520"
     CDS             <1..1821
                     /locus_tag="VFL49_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7911466.1"
                     /translation="ESAAQLCDAEIAAIIREKGDAYFWATSYGLPPDSAEYMKVNVAM
                     KPGRATVAGRVLQEGKTVHLPDVLADADYAALDVQKAIGFRAILGVPLMRAGAPIGII
                     LLMRRVPKPFTDKQIELAETFADQAVIAMENVRLFDEVQMRTRDLTESLQQQTATADV
                     LKTISRSAFDLPTVLDALIETAARMSGADQGAITREIDGAFFRAANYGYSAALSDFIR
                     NTPVLTDRSSIAGRALVEGRIVQIDDVKADPDYTFSPALDSGDFRTALGVPMLREGMP
                     IGVLSLTRKDVRPFSDKQIELASTFADQAAIAIENVRLFDEVQGRTRELAKSLEDLRT
                     TQDRLVQTQKLASLGQLTAGIAHEIKNPLNFVNNFSVVSSELIDELQDTLKGVPFDDK
                     TRAGINELTKILRGNLDKVVQHGKRADAIVRNMLLHSREGSGEHRVVDVNAIVEESLN
                     LAYHGARAEKQGFNITLEKSFDANAGKADLFPQEITRVLLNLIVNGFYAATKRKAEAS
                     GGGYEPTLTAATRSLGDRVEIRIRDNGTGILPEVKEKIFNPFFTTKPAGEGTGLGLSL
                     SHDIVVKQHGGSIEVDTRPGEFTEFRVILPRSGAALAKSE"
     gene            1857..>2175
                     /locus_tag="VFL49_05525"
     CDS             1857..>2175
                     /locus_tag="VFL49_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911467.1"
                     /translation="MRRRDFITLFAGASAAVPFAAFAQKSPRIGWLVFGDASLGSTDQ
                     SLKDALAQNGLVDGRTISILYRFANGDLKRVPALAEELAAEKPDIMIAIGGDLTKALF
                     DASK"
BASE COUNT          463 a          645 c          655 g          412 t
ORIGIN      
        1 gaatcggcgg ctcagctttg cgacgcggaa atagcggcca tcattcgtga gaaaggtgac
       61 gcctacttct gggcgacgag ctatggtttg ccgccggaca gcgccgaata catgaaggtg
      121 aacgtcgcga tgaagccggg acgcgcgacc gtcgccggac gtgtgctgca ggagggaaaa
      181 accgttcacc ttccggatgt ccttgcagat gcggactatg cggctctcga cgttcaaaag
      241 gctatcggct tccgcgccat actcggcgtt cccctgatgc gggcaggagc accgatcggc
      301 atcatcctgt tgatgcgccg agtgccgaaa ccgttcaccg acaagcagat cgagctggcc
      361 gagacctttg ccgaccaggc agtcatagcg atggagaacg tccggttgtt tgacgaagta
      421 cagatgcgca cgcgcgatct gacagagtcc ctccagcaac aaaccgccac cgcggatgtg
      481 ctcaagacaa tcagccggtc ggcatttgac ttgccgacgg tcctcgacgc cttgattgaa
      541 acggcggccc gcatgagtgg tgccgaccaa ggcgccatta cccgggagat cgacggtgca
      601 ttctttcggg ccgcaaatta cggctattcg gccgcgcttt cggacttcat tcgcaataca
      661 ccggtgctga cggatcgcag ctcaattgcg ggccgcgcgc tggtggaggg ccggattgtt
      721 caaattgacg acgtcaaggc tgatcccgat tacacctttt cgccagcgct ggattcaggt
      781 gactttcgca ccgccctcgg ggttccaatg ctgcgggaag gcatgccgat cggcgtcctg
      841 tcgctgacac gcaaggacgt gcgacctttt tccgataaac agatcgagtt ggcttcgacc
      901 ttcgccgacc aggccgcgat cgcgatcgag aatgtgcgcc tgttcgacga agtgcaggga
      961 cgcacgcgcg aactggccaa atcactggaa gatctgcgca ccacgcagga ccgcctggtg
     1021 cagacgcaga aactcgcttc gctcggccag ctcaccgccg gcatcgcgca cgaaatcaag
     1081 aacccgctca atttcgtcaa caatttctcg gtcgtctcca gcgaattgat tgatgaactg
     1141 caggacacgc tgaaaggtgt ccctttcgac gacaagacgc gtgccggaat caacgagctg
     1201 acgaagattc tgcgcggcaa tctcgacaag gtcgtgcaac acggcaagcg cgccgatgcc
     1261 atcgtcagga acatgctgct gcactcgcgc gagggctcgg gtgagcaccg ggttgtggat
     1321 gtcaatgcta ttgtcgagga gagccttaat ctcgcctatc acggggcgcg ggccgagaag
     1381 cagggcttca atatcacgct cgaaaagtca ttcgatgcga acgccggcaa ggccgatctg
     1441 ttcccgcagg agatcacgcg ggtgctgctt aatctgatcg tcaatgggtt ctatgcggcg
     1501 acaaagcgca aggccgaggc aagcggcggc ggctacgagc cgacgctgac ggcggccacc
     1561 agaagcctcg gggaccgcgt cgagatccgc attcgcgaca acggcaccgg tattcttccc
     1621 gaggtgaagg aaaagatctt caacccgttt ttcacgacca agccggccgg tgaagggacc
     1681 gggctggggc tgtccctcag tcacgacatt gtcgtgaagc agcacggcgg atcgatcgag
     1741 gtcgacacca ggcccggaga attcaccgag tttcgtgtca ttcttccgcg ctcgggcgct
     1801 gcgctcgcaa agtcagaatg acgggtaaaa tgcaatcgct cgcgccgaga gctgcaatga
     1861 gacggcgaga tttcatcaca cttttcgctg gcgcttcagc ggcggtgccg ttcgcggcct
     1921 tcgctcaaaa aagcccccgg atcggatggc ttgtctttgg tgacgcttca cttgggtcca
     1981 ccgaccaatc cctgaaagac gcgcttgccc aaaacggcct cgtcgacggc cgcaccatca
     2041 gtatcctgta tcgcttcgcg aatggcgatt tgaaacgtgt tccggcgctt gctgaagagc
     2101 tggctgccga gaagcccgat atcatgattg cgatcggcgg cgacctcacc aaggcgctct
     2161 tcgatgcaag caaag
//
LOCUS       DASSRL010000269         5683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_847046, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000269 DASSRL010000000
VERSION     DASSRL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847046"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3129)
                     /locus_tag="VFL49_05530"
     CDS             complement(<1..3129)
                     /locus_tag="VFL49_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018245221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7911468.1"
                     /translation="MQHFNLSAWAITHRALILFMMIILAGAGIYSYLNLGRAEDPSFT
                     IKVMIVNVSWPGATAAEMEKQVADKIEKKLQELPHLDRVESYSRPGVSYIRVILSDTT
                     PPSQVKELWYQVRKKVADIRNDLPPDIQGPNFNDEFGDVYSALYMLTGDGQSYAELKA
                     RAEDIRQRLLRVPNVNKADIIGEQPQRIYIEFSHAKLATLGITPQQIFDSVARQNAVT
                     SGGSIDTSADRINLRVSGAFSGVEAIAAVPVQADGRVFRLGDIATVKRGYEDPPSFLV
                     RSNGKPALGIGVSMLEGANIIKLGEDLKQAMKSITVELPVGVEITQVADQPHVVDESV
                     SEFFHVFTEALVIVLIVGFVSLGLRTGVVVALSVPLVLAIVFVVMFATGFDLHRITLG
                     ALIIALGLLVDDAIIAIEMMVVKMEQGFDRVRAATFAWTSTAFPMLTGTLVTAAGFLP
                     VGFAKSSAGEYAGGIFWVVGIALIASWFVAVLFTPYLGIKLLPDLKKHGTEHDPNAIY
                     QTRIYRGLRSVIELALRWRKTVVAATVLMFLASIGGFGLVQQQFFPTSTRTELFFEMR
                     LPEGTAIGVTEATAKKAEQLVGNDPDVESYTTYVGAGAPRFWLGLNPELPNTNFAQIV
                     IVAKNLEGRERLKARLEKAIAAGALPQARARVDRFVFGPPVGFPVQFRVIGPDPMKVR
                     AIAEDVRKVMAENHKLVDPHLDWNEQVKSIRLDIDQDRARALGLTPQDISQTLQTLVS
                     GHTITQYREGIEQIAVVARAIPSERLDLDRLPDLTITTRNGVAVPLSQVAKLSYDFEE
                     PILWRRNRDLVLTVRSDVVDGVQPPDVSNEVAPRLQHIKDALPYGYRIETGGSIEESV
                     KANVALVAVFPVMIIAMLMLLMIQLQSFSRLALVVVTAPLGLIGATGALLISNRPFGF
                     VALLGLIALAGMIMRNTVILVDQIDKDIAEGHARYRAIVDATIRRARPVTLTALAAIL
                     GMIPLSRSVFWGPMATTIMGGLLVATILTLLVVPALYALWFRVRKDEPAAEISHEESA
                     AVDREEKPARIAA"
     gene            complement(3214..4296)
                     /locus_tag="VFL49_05535"
     CDS             complement(3214..4296)
                     /locus_tag="VFL49_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7911469.1"
                     /translation="MPKPFTLAGPMAALTAALLLAACQAETAPAPRAERPVQVQSVAY
                     EAEDAARDFVGVVRARYETDLGFRVAGKMVARLVNMGDRVHAGDVVAQLDPEDLRLQV
                     ESAQAELAAATTNLSQISADYERYETLKARGYASIADFDRKAAAKGEAESRLARARRA
                     LDLARNQLDYSELKAGADGVITATLAEPGQVVALGQPVVKLAHRGEKEAVVALPENWL
                     GKARAAVATVTLWSGNGRRYAARLRELSPQADPATRTYAARFTILDPDDSVALGMTAT
                     VSLKPAGEAMVAKLPLSAVLSRGSGASVYVVNQAGELILRPVTVASFNEDDALITGGV
                     SAGEKVVTLGVQKLEPGLKVRSIETK"
     gene            complement(4392..5000)
                     /locus_tag="VFL49_05540"
     CDS             complement(4392..5000)
                     /locus_tag="VFL49_05540"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7911470.1"
                     /translation="MNALPRTKPDDTRDRIMDVADRLFRQVGFAKTTVGDIAAELTMS
                     PANVYRFFPSKNAIVEAICRRCLAELDERAWEIARSRGSAAARLERLILEILAYHKAN
                     LLTDKRVNDIVLVAIEDSWDAIQAHKEIIRNVVELILRDGIAAGEFEKVDPAETSQLL
                     MRSLVAYVHPVLISQCLTDEEDVEAQAKAGIRFSLRAITPRT"
     gene            complement(5116..5358)
                     /locus_tag="VFL49_05545"
     CDS             complement(5116..5358)
                     /locus_tag="VFL49_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911471.1"
                     /translation="MTVRTILSALAVLAATTSMGFAQSLPNYGPNAPATGDSFGKPPS
                     ATRSPGVTRSQRSAYAYQRDHRYHRYRHKRVQQPYD"
BASE COUNT         1029 a         1774 c         1815 g         1065 t
ORIGIN      
        1 ggctgcaatg cgtgccggtt tctcctctct gtcgactgcc gccgactcct catgcgatat
       61 ttccgctgcc ggctcatctt ttctgacacg gaaccacagc gcatacagtg ccggcacgac
      121 cagaagcgtc aggatggtcg caaccagcag gccgcccatg atggtggtcg ccatggggcc
      181 ccagaacacg ctgcgcgaga gcgggatcat gccgaggatg gcggcgagcg ctgtcaaggt
      241 caccgggcgg gcgcgccgga tcgtcgcgtc gacgattgcc cggtagcggg catgaccttc
      301 ggcgatatcc ttgtcgatct ggtccaccag gatcacggtg ttgcgcatga tcatcccggc
      361 aagtgcgatc aggccgagca gagccacgaa gccaaacggc cggttggaga tcagcagcgc
      421 gccggttgcc ccgatgagac cgagcggcgc ggtcacgacc acgagcgcca gccgcgagaa
      481 gctctgcagc tggatcatca gcagcatcag catcgcgatg atcatcaccg ggaaaacggc
      541 gaccagggcg acgttcgcct tgacgctttc ctcgatcgat ccgccggttt cgatcctgta
      601 gccataaggc aaggcgtctt tgatgtgctg gagcctgggc gcaacttcat tgctcacgtc
      661 cggcggctgc acgccgtcga cgacgtcgct gcgcaccgtc aggaccagat cgcggttgcg
      721 ccgccacagg atcggctctt cgaaatcgta agagagcttg gccacttgcg acagcggcac
      781 cgcgacgcca ttgcgggtcg taatcgtgag gtccggcaac cggtcgagat cgagccgttc
      841 ggacggaatg gcgcgcgcca caactgcgat ttgctctatt ccttcgcggt actgcgtgat
      901 cgtatgaccc gagaccagcg tctgcagggt ctgcgagata tcctgcggtg tcagccccag
      961 ggcccgcgcc cgatcctggt cgatgtcgag ccggatcgat ttcacctgct cgttccagtc
     1021 gagatgcgga tcgacgagct tgtggttttc cgccatcacc ttgcggacgt cttcggcgat
     1081 ggcccgcact ttcatcgggt cgggaccgat gacgcggaat tgcaccggga aaccgaccgg
     1141 tgggccgaac acgaagcggt cgacgcgggc gcgcgcctgc ggcagggcgc cggcggcgat
     1201 cgccttttcc agccgcgcct tgaggcgctc gcgcccctcg agattctttg cgacgatgac
     1261 gatctgcgcg aaattggtgt ttggcagttc cggattgagc ccgagccaga agcgcggggc
     1321 gccggcgccc acgtaagtcg tatagctttc gacgtccggg tcgttgccga cgagttgctc
     1381 cgcctttttg gccgtcgcct cggtgacgcc gatcgccgtt ccttccggca ggcgcatctc
     1441 gaagaacaat tcggtgcggg tcgatgtcgg gaagaactgc tgctgcacca ggccgaagcc
     1501 gccgatgctg gcgaggaaca tgagcaccgt cgccgccacc accgtcttgc gccagcgcag
     1561 ggcaagctcg atcaccgaac gcagtccgcg gtagattcgc gtctgataga tcgcgttcgg
     1621 atcgtgttcc gtgccgtgtt tcttcagatc gggcagcagc ttgatgccga gataaggcgt
     1681 aaacagcacc gccacgaacc acgacgcgat cagcgcgatg cccaccaccc agaaaatacc
     1741 gccggcatat tcgccggcgc tcgatttggc gaagccgacc ggcaggaagc ccgccgccgt
     1801 caccagcgtc ccggtcagca tcgggaatgc cgtggacgtc catgcgaagg tcgccgcgcg
     1861 cacccgatca aacccctgct ccatcttcac caccatcatt tcgatggcga tgatcgcgtc
     1921 atccaccagc agcccgagcg cgatgatcag cgcgcccaaa gtgatgcggt gcagatcgaa
     1981 gccggtcgcg aacatcacga caaagacgat ggccagcacc agcggcaccg acaacgccac
     2041 gacgacgccc gtgcgcaggc ccagcgagac aaagcccacg atgagaacga tcaccagcgc
     2101 ctcggtgaac acatggaaga attccgacac cgattcgtcg accacgtgcg gctgatcggc
     2161 cacctgcgtg atttccacgc cgaccggcag ctcgacggtg atggatttca tcgcctgctt
     2221 gaggtcttcg ccgagcttga tgatgttggc gccctcgagc atcgacacgc cgatgccgag
     2281 cgccggcttg ccgttgctgc gcacgaggaa gctcggcggg tcctcatatc cgcgcttcac
     2341 cgtcgcgata tcgccgagcc ggaaaacgcg gccgtcggcc tgtacgggca ctgcggcaat
     2401 ggcttcgacg cctgaaaaag ccccgctcac gcgcagattg attcgatcgg cggaggtgtc
     2461 gatcgagccg cccgaggtca cggcattctg cctggcgacg ctgtcgaaga tctgctgcgg
     2521 cgtgatgccg agtgtcgcca gcttggcgtg gctgaactcg atgtagatgc gctgcggttg
     2581 ctcgccgatg atgtcggcct tgttcacgtt cggcacgcgc agcaggcgct ggcggatatc
     2641 ctcggcgcgc gccttgagct cggcgtagct ctggccgtcg ccggtgagca tgtaaagcgc
     2701 cgagtagacg tcgccgaatt cgtcgttgaa attcggtccc tggatgtccg gcggcaaatc
     2761 gttgcggatg tccgccactt tcttgcggac ctggtaccag agctccttca cctgcgaggg
     2821 cggcgtcgtg tcactgagga tgacccggat gtaggaaacg cccggccgtg aatagctttc
     2881 cacgcggtcg aggtgcggca attcctgcag cttcttctcg atcttgtccg cgacctgctt
     2941 ctccatttcc gccgcggtgg cgcccggcca cgacacgttg acgatcatca ccttgatagt
     3001 gaacgagggg tcttcggcac ggccgagatt gaggtaggag tagatgccgg cgcccgccag
     3061 aatgatcatc atgaagagga taagcgcgcg atgcgtgatg gcccaggcgg acagattgaa
     3121 atgttgcatg gcgccgttac tccgtcagga aggacttcgt tgggacttgc aacttcgctg
     3181 cacgggctgc gactgcccgt gaagcatcgc gaattatttc gtctcgatgc tgcggacctt
     3241 gagcccgggc tcgagctttt gcacgccgag cgtcactact ttctcgccgg ctgaaacgcc
     3301 gccggtgatg agcgcgtcgt cttcgttgaa cgaagcaacg gtgacgggtc gcaagatcag
     3361 ctcgccggcc tggttgacca cgtagaccga cgcgcccgag ccgcggctga gcaccgcaga
     3421 caaaggcagc ttcgcgacca ttgcttcgcc ggctggtttg agcgagaccg tcgccgtcat
     3481 tccgagcgca acgctgtcgt cgggatcgag gatcgtgaaa cgcgccgcgt aagttcgcgt
     3541 cgccgggtcg gcctgaggcg agagctcgcg caaccgggcg gcgtagcggc ggccgttgcc
     3601 ggaccaaagc gtgacggtcg cgaccgccgc gcgtgccttg ccaagccaat tttcaggcag
     3661 cgcgacgact gcttcctttt ctccgcgatg ggcaagtttc accaccggct ggccgagagc
     3721 gaccacctgc cccggctccg ccagcgtcgc cgtgatgacg ccgtcggcgc cggccttgag
     3781 ctcggaatag tcaagctgat tgcgcgcgag atcgagcgcg cgcctggcgc gcgcgagcct
     3841 gctttcggcc tcgcctttcg ccgctgcctt gcggtcaaaa tcggcaatcg acgcatagcc
     3901 gcgagccttc agggtctcgt agcgctcata gtcggcggag atctggctca gattcgtggt
     3961 tgccgcggca agctccgctt gcgcgctttc gacctgcagc cgcaggtctt cgggatcgag
     4021 ctgcgcgacc acgtcgccgg cgtgcacgcg gtcacccatg ttgacgagcc gcgcgaccat
     4081 tttgccggcg acccggaagc cgagatcggt ttcgtatcgc gcccgaacga caccgacaaa
     4141 gtcgcgcgca gcgtcttcgg cctcataggc aacgctttgc acctggaccg ggcgctcggc
     4201 tctgggggcc ggtgcggttt cggcctggca cgcggccaaa agaagtgccg ccgtcaacgc
     4261 cgccatgggt ccggcaaggg taaaaggctt gggcatggcg gggctccttt cactgagatg
     4321 tgacgagtga tgaaatctca ttttcgtcac actttagaca cgcgaaatct tggagggcgg
     4381 acggcatcgg gtcaggtccg gggagtgatg gcgcggaggg agaagcgtat gccggccttg
     4441 gcctgtgcct cgacgtcttc ttcgtcggtc aggcactggc tgatcaggac cgggtgcacg
     4501 taggcgacca gggaccgcat cagaagctgc gaggtctcgg caggatcgac cttttcgaac
     4561 tcgccggcgg cgatgccgtc gcgcaggata agctcgacca cgttgcggat gatttccttg
     4621 tgtgcctgga tggcgtccca actatcttcg atcgcgacca gcacgatgtc attcacgcgc
     4681 ttgtccgtaa gcaaattggc cttgtgatag gccaggatct cgaggatcag ccgttcaagg
     4741 cgcgcggcgg ccgagccgcg cgagcgggcg atttcccagg cccgttcgtc gagctcggcg
     4801 aggcagcgcc ggcagatcgc ttcgacgatc gcgttcttgg aggggaagaa ccgatagacg
     4861 ttcgccggag acatagtgag ctccgcggca atatcgccga ccgtcgtttt ggcgaagccc
     4921 acctgccgga acagcctgtc ggccacatcc atgatgcggt cgcgggtatc gtcgggcttg
     4981 gtccttggca gggcattcat ggaactgaca ctctgatgag tgatgaaatt gataattcgt
     5041 cactcatcat ttgtcaatac cccgaccctg cccgattccg ccaccgggcc ggcaggctgt
     5101 tttgacgggc tgtgcctaat cgtaaggctg ctgcacgcgc ttgtgccggt agcggtgata
     5161 ccggtgatcg cgctgatagg cgtaggcgct gcgctgcgag cgggttacgc cgggtgaccg
     5221 ggtggcgctt ggcggcttgc cgaagctgtc gccggtcgcc ggggcgtttg gtccgtagtt
     5281 cggcaggctc tgcgcgaagc ccatcgaagt cgtggcggca agcaccgcca gtgcggaaag
     5341 aatcgttctc actgtcatgg tccaatctcc agcatccgtt tctctggaga acccactgga
     5401 gaccgcctgg ttcgacacgc tgctcggttg ctcttgcttc aagacaagct tctgtgcatc
     5461 ggccttcgat attggccatg gcacgcccct aacgaaaaga gcagccgcta acgggatatt
     5521 cccagcagca cggaaattat ctcggatgca cccatcaccc cgaaaaccat aacgacaggc
     5581 gggtaaagta cgagtgcggg ttcaaaccga agaaatcgcg ggtgttgacc ggcgcgattt
     5641 ggccgtccga tgaaaacaag aaagatagcg agtgccacca acg
//
LOCUS       DASSRL010000270         5633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1302056, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000270 DASSRL010000000
VERSION     DASSRL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302056"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VFL49_05550"
     CDS             complement(<1..1164)
                     /locus_tag="VFL49_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911472.1"
                     /translation="MLLCLNCQASAADQRRRIFFLESLSPTLPAAVRTIDAFKKRLSE
                     KTSEQFDIFVDYMELVRLPGQAHIDRTVRYLSGRYAEARPDVLITLGRAAIPFMVKYR
                     DTIAPDVPLILANVPIRDVKESHLKNVFWVTTEYNFSKTLELAQELQPDARHLVVVGG
                     AGTYDQRWLDDARRELKPFSERYTTRYIVGRSYEDTLKEVARLPKNTIVIMSFFFVDG
                     SGQTYSSSKVAADVARVSPAPVYSPISTNLGDGIVGGYTDSWEAHGIATADVALQILA
                     GKPLETIQRENKPEQAYRLDARQLTRWNINASRSPAGSDIHNRQFNVWERYRWQIMAI
                     LATVLLQAAVITGLFFERRRRHLAELELRERLMEVIHLNRTAVAGALSASVAHE"
     gene            complement(1332..3800)
                     /locus_tag="VFL49_05555"
     CDS             complement(1332..3800)
                     /locus_tag="VFL49_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7911473.1"
                     /translation="MASRRYKKAGQPAPNNRGDKDAAVESSLSTRKRFAPLIRLAMTS
                     VAAGFIVILTFGFLAPRWSLFPAAPQAEPPAATFVGSEICAGCHRVETALWRDSQHKH
                     AMDHATEKSVLGDFSGATFDYYGVRSRFFRKDGKYFVDTDGPGGKLAVFQVKYTFGID
                     PLQQYLIEFPDGRVQALSIAWDARSKEKGGQRWFHLYPDENIRHDDVLHWTKLNQNWN
                     FMCAECHSTGLRKNYDADHDRYATTWTEISVGCEACHGQGSNHVAWARAQQSWLLFNR
                     TEDRGKGLLARLNEREGIIWTHDPAGGNVRRNLPANILRTEVETCGRCHARRSEISEK
                     WVPGHWLQDTHAIAASLAGDVYWPDGQMRDVAEAYNYVPFKQSKMFAAGVTCSNCHDP
                     HSGRQRIADDGVCLQCHADEVYAGTRHSHHKQADAAPNCVSCHMPARTYMVIDKRHDH
                     SLRIPRPDLSVKHGTPNACNDCHTDKAAQWAADTIDRWFGPDRKGFQTYAEAFAAASA
                     DRADARQRLASVAGDTNVPGVARAQALIDLAPSISPANADLARRSLADPDPMVRIAAM
                     DMLANVPAAQVWPLVAPLLSDSVLGVRIRAASLLAAVPTERQPQADRERFERAAAEFV
                     AALKLNADRPEDRSTLANFYARQGRSAEAEAEYKAALRLSPQYVVAAINLADLYRQVG
                     RDADGESALRAAIAVSRGDAGLHYALGLTLNRLKRSDDSLVELGRAAELDPDRARYAY
                     VYAVGLHSAGRVGDAIAALKNNLDKHPADRDSLLALVTFNRDSGNVASALDYAERLAR
                     LEPADRRISELVETLKRQLESPSR"
     gene            complement(3863..4189)
                     /locus_tag="VFL49_05560"
     CDS             complement(3863..4189)
                     /locus_tag="VFL49_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911474.1"
                     /translation="MKKAGALVVTVFFGLLSDAAFADDVPKLDFRKSCRTDVAAYPAG
                     GGNKACLSDEQKARKTLVSQWKRFSPQSRARCTQMVTDMVGSQSYVELLTCLQMAIDS
                     KSLPKM"
     gene            complement(4250..4732)
                     /locus_tag="VFL49_05565"
     CDS             complement(4250..4732)
                     /locus_tag="VFL49_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HET7911475.1"
                     /translation="MTRFMSVLAAFVLAVLVAPASAQSGKPWTQVGMLTCKLNPSIGF
                     VIAGHQSMECRYVPNTGTQPQAYQGALNMVGINIGISAGGILNWAVLAPTDGIPAGAL
                     AGEYVGASGDVGIGVGVGANVLLGGSGRTLALQPVSVEGSVAANVALGVSALKLRPAI
                     "
     gene            complement(4809..>5633)
                     /locus_tag="VFL49_05570"
     CDS             complement(4809..>5633)
                     /locus_tag="VFL49_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7911476.1"
                     /translation="WGVNEAGMKQMDDNIGVVLKKIDDMGQTNNTIVVFTTDNGAETI
                     TFPDGGTTPFKGGKLTTWEGGMRAPMVIRWPGVIRPGTVHSQLFSALDWVPTLVDIAG
                     GPKGDALKRRIEAGQYPGIVKTTLDGLDQRDFLEGKSKSARDTIMYYSGKDPSAVRYK
                     NWKMYFAMVSDAPAGFVAGVLPYHWTQVVNIKRDPFETSVGQQQKTLFGMGGAIASPS
                     TAYVYDWNLLPIGQALWLKELETYVKFPPLQAPSSYNLEQVMQQVREMKTASHAGE"
BASE COUNT         1103 a         1708 c         1682 g         1140 t
ORIGIN      
        1 ctcatgcgcg acggaggccg acaacgcgcc tgcaaccgcc gtccggttca ggtggatcac
       61 ctccatcagc cgctcacgca attcaagttc tgccaggtgc cggcggcgac gttcgaaaaa
      121 caggcctgtg ataacggccg cctgcaggag gacagtcgcg agaatcgcca tgatctgcca
      181 gcgataccgt tcccacacat tgaattgacg attgtgaatg tccgaccccg caggactgcg
      241 tgaggcattg atgttccacc gcgtcaactg gcgcgcatcc agacggtatg cctgctcagg
      301 cttgttttcg cgctgaatgg tttcgagcgg tttgccggcc agaatttgca aggcgacatc
      361 cgctgtcgca atgccgtgcg cctcccagct atccgtgtat ccgcctacga taccgtcgcc
      421 cagattggtg ctgatcggcg agtacaccgg cgccggcgaa acccttgcca catcggcggc
      481 gactttggac gaagagtatg tttggcccga accatcaaca aagaagaatg acatgatgac
      541 aattgtgttt ttcggcagtc gagcaacctc cttcagggta tcctcgtagg agcggccaac
      601 gatgtatctt gtcgtgtagc gttcgctgaa tggcttcagc tcgcggcgcg catcgtccag
      661 ccatcgctgg tcgtaggtgc cggcaccgcc aaccacgaca agatggcgcg cgtccggctg
      721 cagttcctgt gcaagctcca gggtttttga gaaattgtat tcggtggtga cccagaagac
      781 gtttttcagg tgactttctt tcacatcgcg gattggcacg ttggcaagaa tgagcggtac
      841 atcaggcgca atagtgtcgc gatatttgac catgaagggt atagcggcgc gccctaacgt
      901 tatcagcacg tccgggcgcg cttcggcgta cctcccggaa agataccgca ccgtgcgatc
      961 gatgtgcgcc tggcccggca accggacaag ctccatgtaa tcgacaaaga tatcaaattg
     1021 ctcgctcgtc ttttcgctaa gtcgtttctt gaaggcatca atggtgcgca ctgccgcggg
     1081 cagcgtcggc gaaaggcttt ccagaaaaaa gatgcgccgc cgttgatcgg cggcgctcgc
     1141 ctggcagttc aggcaaagca acacgacgaa cgcgacaatg gatgttcgta ccggagaggc
     1201 tcgacgaatt tcggtcattc cggttgctgt gtcgcggtcc aggcatggtc ccaccgttgc
     1261 tattttactt tcgtgacatt ctggctaaat tggaccatgg gccaatttgg atggccgatt
     1321 tggactggtc tttatcgcga tggagactcg agctggcgct tgagcgtctc gaccaactcc
     1381 gaaatccgcc tgtcggcggg ttcgagccgg gccaggcgct ccgcgtaatc caaagccgac
     1441 gcgacatttc cgctgtcacg attgaacgtg acaagggcca gcaacgagtc tcgatccgcc
     1501 ggatgcttgt ccagattgtt ctttagggcg gcgattgcgt ctcccacccg ccctgcggaa
     1561 tggagaccaa cagcatagac ataggcatat cgtgccctgt ccggatcaag ctcagcagcg
     1621 cggccaagct caacaagcga atcatcagat cgcttcaggc ggttgagggt cagtccgagc
     1681 gcgtaatgca gcccggcatc gccgcgcgaa acggcaatgg ccgcccgcaa cgcgctttca
     1741 ccgtcggcgt cgcggccgac ctgccggtac agatcggcga gattgattgc tgccaccacg
     1801 tactgcgggc tcagccggag cgccgccttg tattcagctt cggcctccgc gctacggccc
     1861 tgccgcgcat agaagttcgc cagtgtcgaa cggtcctccg gacgatcggc attgagctta
     1921 agcgcagcga caaactcggc cgcggcccgc tcgaagcgtt cacgatcagc ctgcggctgg
     1981 cgctcggtcg gaaccgcggc gagcagactg gccgctcgaa tacgcacgcc aagaaccgaa
     2041 tccgacagaa gcggcgcaac cagtggccag acctgcgcag ccggcacatt ggccagcatg
     2101 tccatggcag caatgcgcac catcggatcg ggatcggcaa ggctgcggcg cgccaggtcg
     2161 gcattcgccg gcgagatgga gggcgcgaga tcgatgagcg cttgcgcacg cgcaacacca
     2221 ggcacgttgg tatcgccggc gacagatgcc aggcgctgcc gggcatcggc acgatcggcc
     2281 gaagctgcgg caaaggcttc cgcataagtc tgaaaccctt tgcgatcggg gccgaaccag
     2341 cgatcgatcg tatccgcagc ccattgcgcc gctttgtcgg tgtggcaatc gttgcaagca
     2401 ttaggagtac cgtgcttcac agacaaatcc ggccgcggta tgcgcaggct gtggtcgtgg
     2461 cgtttgtcga tcaccatgta cgtgcgcgcc ggcatgtgac acgacacgca atttggcgcg
     2521 gcatccgctt gcttgtgatg actgtgcctg gtgcccgcat agacttcgtc cgcatggcat
     2581 tgcaggcaga cgccgtcgtc cgcaatcctc tgcctgccgc tgtgaggatc atgacagttg
     2641 ctgcaggtca cacccgccgc aaacatcttg ctctgcttga aagggacata attataagct
     2701 tcggcgacat cgcgcatttg gccatcgggc cagtagacgt cccctgcaag cgaggcggca
     2761 atcgcgtgcg tgtcctgcag ccagtgtccg ggcacccatt tctcggatat ttcgctgcgt
     2821 cgggcgtggc agcggccgca agtttcaact tccgtccgta gaatatttgc cggaagattg
     2881 cgccggacgt ttccgccggc aggatcgtgc gtccaaatga ttccttcccg ctcgttcagg
     2941 cgagcgagca ggcctttgcc gcgatcctct gtccggttga aaagcaacca gctctgctgc
     3001 gcgcgagccc aggcaacatg gttggacccc tgtccgtgac acgcctcgca gcccacgctg
     3061 atttcagtcc acgtcgtcgc gtagcggtca tggtcggcgt cgtaattctt ccgcagcccg
     3121 gttgaatgac actcggcgca catgaaattc cagttctgat tgagcttcgt ccaatgcagg
     3181 acgtcgtcat ggcggatatt ttcgtcggga tagagatgaa accagcgttg tccgcccttt
     3241 tctttcgagc gggcatccca ggcaatcgag agagcctgca cgcgcccgtc cggaaactcg
     3301 atcagatatt gctgcagcgg atcgatgccg aaggtgtact tcacctgaaa gacggcgagt
     3361 ttgccgcccg gtccgtccgt atcgacaaaa tacttgccgt ccttccggaa gaagcgcgag
     3421 cggacgccgt aatagtcgaa agtcgcgccg ctgaaatcgc cgagcaccga tttctccgtc
     3481 gcgtgatcca tcgcgtgctt gtgctgcgag tcgcgccaca acgcggtttc aacccggtga
     3541 catccggcgc agatttcact gccgacaaaa gtcgccgcag gaggctccgc ttgcggcgca
     3601 gctggaaaca atgaccatct gggcgctaag aatccgaatg tcagaatgac gatgaacccg
     3661 gcagcgacgc tcgtcatcgc gagccggatc aatggcgcga aacgcttgcg cgtggaaagc
     3721 gagctttcga ccgcggcatc cttgtcgccg cggttgttgg gcgccggttg tccggctttt
     3781 ttataccgcc tggaggccaa tgatcgccct cgccgagcta tgcagcctta agctgacccg
     3841 gataacgacg gcttgccaga aatcacattt ttggaagcga tttgctgtct attgccattt
     3901 gcaggcaggt cagcaattcg acgtagctct gtgagcccac catgtcggtt accatctggg
     3961 tgcaacgagc ccggctttgc ggcgagaagc gcttccactg actgacgagc gtttttcggg
     4021 ccttctgttc atcggataga caagccttgt tgccgccgcc tgccggatag gccgcaacgt
     4081 ccgtccggca actcttccgg aaatccagct tcggtacgtc atctgcgaat gccgcatccg
     4141 agagaagacc gaagaatact gtaacgacca acgcgccggc ttttttcatg ctcagccccg
     4201 aaaaaagagt gtcatgcggc agtaacccgg cggagcatac atcattttgt cagatagctg
     4261 gccgcaattt gagggccgaa acccccaggg ctacgttggc cgccaccgag ccttcgaccg
     4321 agacgggctg cagcgcaagc gtccggccag agccgccaag cagaacgttg gcgccaacac
     4381 cgacgccaat gccgacatcc ccggatgcgc cgacatactc gcctgcaagc gcgcccgccg
     4441 gtattccgtc ggtcggcgcg agcaccgccc aattaaggat gcctccagcg ctgatgccga
     4501 tgttgatccc gaccatgttc agcgcaccct ggtaggcctg cggctgagtg ccggtattcg
     4561 ggacgtaacg gcattccatg gattgatgcc cggcgatgac gaagccgatg ctcgggttga
     4621 gcttgcatgt cagcatgccg acctgcgtcc agggttttcc cgattgagcg gatgccggag
     4681 cgacaaggac tgcaagcacg aaagcggcga gcacggacat aaatcgagtc attggcttgc
     4741 tccgaaatga atcacttcca ggaaggactg cggggcgccc gttggtgggc gcccgcatcg
     4801 gcttggcatt attcgcctgc gtgactcgcc gtcttcattt cccggacctg ctgcatgacc
     4861 tgctccaggt tgtagctgga cggagcctgc agcggcggga atttgacgta ggtctccagc
     4921 tccttcagcc acagcgcctg gccgatcggc aggagattcc agtcatagac ataggcggtc
     4981 gacggagaag caatcgctcc gcccatacca aagagcgtct tctgctgctg accgacggac
     5041 gtctcgaagg ggtcgcgttt gatattgacg acctgggtcc aatggtaggg aagcacgccg
     5101 gcaacgaaac ccgccggggc gtcggatacc atcgcgaagt acatcttcca gttcttgtag
     5161 cggaccgctg aaggatcctt gccggaatag tacatgatcg tgtcacgcgc tgatttcgac
     5221 ttcccttcca ggaagtcgcg ctggtcgagg ccgtcgagcg tggtcttgac gatgccgggg
     5281 tactggccgg cctcgatccg tcgcttcagc gcgtcaccct tgggaccgcc ggcgatgtcg
     5341 accaaggtcg gcacccagtc gagcgcggag aacagctgac tgtgcaccgt gcccggcctg
     5401 atgacgcccg gccagcggat caccatcggg gcgcgcatgc cgccttccca ggttgtcaac
     5461 ttgccgccct tgaaaggggt ggtgccgccg tccgggaagg tgatggtctc ggcgccattg
     5521 tcggtggtga agacgacgat ggtattgttc gtctgcccca tgtcgtcgat tttcttgagc
     5581 acgacgccga tgttgtcgtc catctgcttc atgccggctt cgttgacgcc cca
//
LOCUS       DASSRL010000271         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_112394, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000271 DASSRL010000000
VERSION     DASSRL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112394"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..560)
                     /locus_tag="VFL49_05575"
     CDS             complement(96..560)
                     /locus_tag="VFL49_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7911477.1"
                     /translation="MNQRADHSFNEYVTAMIGGQLFGLPILRVQDVFAPERLTAVPLS
                     PPEVAGVLNLRGRIVTLIDMRCRLGLDDRENDGRQSMAIGVESRGESYGLLIDSVGEV
                     LKLDSLAWEPNPTNLDARLASVSTGIFRLDSQLLMILDVDRVLDITVKAVAA"
     gene            complement(572..3196)
                     /locus_tag="VFL49_05580"
     CDS             complement(572..3196)
                     /locus_tag="VFL49_05580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET7911478.1"
                     /translation="MDDLLREFVTETNESLDVVDVELVRFEQDPNNAKILDNIFRLVH
                     TIKGTCGFLGLPRLEALAHAAETLMGKFRDGVPASSEAVTLVLATIDRIKIILESLES
                     DQREPEGVDDDLITSLEGMVDRISAGGARPEAEHTVGTLVHQVLERPLREDEDTLDDL
                     EHAFRSAVAESGHANSVKPALNAADPAIKDDEAGKSANQSIRVTVDTLEQLMTMVSEL
                     VLTRNQLLEIVRRHEESEFKAPLQRLSNVTAELQEGVMKTRMQPIGNAWQKLPRIVRD
                     LSNELGKKIDLEMQGADTELDRQVLDLIKDPLTHMARNAADHGLETVDERRARGKPEH
                     GLIRLSAYHEGGYIIIRVADDGRGLDTERIKAKAIAQGLVSEADAEKLPEAQIHKFIF
                     APGFSTAAKITSVSGRGVGMDVVRNNIDQIGGTIDVKSVAGAGLSFTIKIPLTLAIVA
                     ALIVEAGGHRFAIPQLSVVELVRARSGSEHRIESIKSTRVLRLRDKLLPLVSLRHLFG
                     LDKGTGEIDGGFIVVTQVGSQTFGIVVDGVFHTEEIVVKPTSSKLRHIAMFSGNTILG
                     DGSVIMIIDPNGVAQAFGSNVTSQLAAAEASEIKPTPAAGASTSLLVFRAGSMQPKAV
                     PLSLITRLEEIDAQKIELSNGRHMVQYRGHLMPLISMSDNVRVKGEGAQPLLVFSDGS
                     RSMALVVDEIVDIVDDRLDIQVASENPGVLGSAVVRGQATEIIDVGHFLPLAFEDWFR
                     RKEEQVQHRPRTILLVDDSPFFLNMLTPVLQAAGYAVTTAASARDALALLAEGQTFDV
                     AVTDIEMPDMNGFQFAEAVRRDPRTGDLPIIALSSVVSPEAIERGRRVGFYDYVAKFD
                     RQGLIAALKEQTGVVQAA"
     gene            complement(3352..>3721)
                     /locus_tag="VFL49_05585"
     CDS             complement(3352..>3721)
                     /locus_tag="VFL49_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HET7911479.1"
                     /translation="ISRGFLEDEKTKLAWNLPRALLQKNRVKLLLNMLAIGGQTIPRG
                     GALTVDPIGSGETMGFKVTAAGINAKVPPAVSVLLGGETAEGLDAHRIQPFYAGLLAK
                     ACGVKAAIAMDGEKVVITAQ"
BASE COUNT          689 a         1157 c         1115 g          760 t
ORIGIN      
        1 tccggatgac gctggagtcg tcaacgacca aacaggtttt catcgtatag cctctttgtg
       61 ttctcgccca attcccattt ccagctccga ccgcattacg ctgcgaccgc cttcaccgtg
      121 atatcgagca cacgatcgac gtcgagaatc ataagcagtt ggctatcaag ccgaaaaatt
      181 ccggttgata cgcttgccaa acgtgcatcg agattggtcg gatttggctc ccaggccagg
      241 ctgtccagct tgaggacttc gccgacgctg tcgatgagca agccgtagga ttcgccccgg
      301 gattcgacac cgatcgccat cgactgccgg ccgtcatttt cccggtcgtc gagtccgagg
      361 cggcatcgca tgtcgatcag cgtcacgatg cggccccgca gattgaggac gcctgccact
      421 tccggcggcg aaagcggcac ggcggtaagg cgctccggcg cgaagacgtc ctgcacccgc
      481 aggatcggca agccgaacaa ctggccgccg atcatggcgg tcacatattc gttgaagcta
      541 tggtcggctc tctggttcat gctgtgctct gtcacgcggc ctggacaacg ccggtttgct
      601 ccttgagcgc cgcaatcagc ccttggcgat caaacttcgc cacgtagtcg tagaagccga
      661 cgcgccgtcc gcgttcaatt gcctccggcg aaaccaccga ggacagcgcg ataatcggta
      721 gatcgcccgt tcgggggtcg cgccgcacgg cctcggcgaa ctgaaagccg ttcatgtccg
      781 gcatttcgat gtcggtcacg gctacgtcga atgtctgccc ctccgcgagc agtgccagcg
      841 cgtctcgcgc cgacgcagcc gtggtcacgg catatccggc tgcctgcagc acgggcgtga
      901 gcatgttcag aaaaaacgga gagtcgtcca cgaggagtat tgtgcgcggg cgatgctgta
      961 cctgctcctc cttgcggcga aaccagtcct cgaacgcgag tggcagaaag tgcccgacgt
     1021 cgatgatttc ggtcgcttgc ccgcgcacga cggctgagcc gagcacgccg gggttttcac
     1081 tcgcaacctg gatgtcgagc ctgtcgtcga cgatatcgac gatttcgtcc acgaccagcg
     1141 ccatggaccg gctgccatcc gaaaacacca ggagtggctg cgcaccctcc ccttttacgc
     1201 gcacattatc gctcatgctg atgagcggca tcaggtggcc gcgatactgg accatgtgac
     1261 ggccattcga cagctcgatt ttctgcgcat cgatttcttc aaggcgcgta atgagcgaaa
     1321 gcggaacggc tttcggctgc atcgatccgg cacgaaacac caagagtgat gtactcgcgc
     1381 ctgcggctgg cgtcggcttg atttcggaag cttccgcggc ggccaattga gacgtgacat
     1441 tcgagccgaa cgcctgcgca acgccgttgg gatcgatgat catgatcaca gagccgtcgc
     1501 cgagaatggt gttgccggag aacatcgcga tatggcgcag cttgctcgac gtcggcttca
     1561 caacgatttc ttcggtatgg aatacaccgt caacgacaat gccgaaggtc tgactgccga
     1621 cctgggtgac gacgatgaat ccgccgtcga tctcgccggt gcccttgtcg agaccgaaca
     1681 aatggcgaag gctgacgaga ggcaggagct tgtcgcgcaa ccgaagcacc cgcgtgctct
     1741 tgatgctctc tatgcgatgc tcgctgccgc tgcgcgcccg cacaagctcg acgaccgaca
     1801 gttgcgggat cgcgaaacga tgcccgcccg cttcgacgat cagcgcagcc acgatcgcga
     1861 gggtaagggg gattttgatg gtgaagctta gcccggcgcc ggcgaccgac ttcacgtcga
     1921 tcgttccgcc gatctggtcg atattgttgc gcacgacgtc catgccgacc ccgcggccag
     1981 acacgctggt gatcttggcc gccgtggaaa agccgggcgc gaatatgaac ttgtgaatct
     2041 gggcttccgg cagtttttcc gcgtcggcct cggagaccag gccctgcgcg atggcctttg
     2101 ccttgatgcg ttcggtgtcg agcccgcgcc cgtcgtcggc gacccgaatg atgatgtagc
     2161 cgccctcgtg ataggccgaa aggcggatca gcccatgctc gggcttacct ctggcacgcc
     2221 gctcatcgac tgtttcaagg ccgtgatccg ccgcatttcg cgccatgtgt gtcagcgggt
     2281 ccttgatcag gtccagaacc tgacggtcga gctcggtatc ggcaccctgc atctcgaggt
     2341 cgatcttctt gccgagctcg ttggaaagat cacgcacgat gcgcggcaac ttctgccagg
     2401 cattgccgat cggctgcata cgcgtcttca tcacgccttc ctgcaactcg gcggtgacgt
     2461 tcgacagtcg ctgcagcggt gccttaaact cggattcctc gtggcggcgc acgatctcga
     2521 gcagctggtt gcgcgtgagc accaactccg agaccatggt catgagttgc tcaagcgtat
     2581 cgacggtgac gcggatcgac tgattggccg acttgccggc ctcgtcgtcc ttgatggccg
     2641 ggtcggcagc gttcaatgca ggcttgactg agtttgcatg tccggattcg gcaactgcgc
     2701 tgcgaaacgc atgctcgagg tcgtcaagcg tatcctcgtc ctcacgcaag ggccgttcga
     2761 gaacctggtg aaccagcgtt ccgacagtat gctcggcttc cggcctggct cccccggcgc
     2821 tgatgcgatc gaccatgccc tcaaggctgg tgatgagatc atcgtcaacg ccttcgggct
     2881 cgcgttggtc gctctcgagc gactccagaa tgatcttgat ccggtcgatg gtcgcaagaa
     2941 caagcgtgac ggcttcgctc gacgccggaa cgccgtcgcg aaacttgccc atgagagtct
     3001 cggcggcatg ggcaagcgcc tcgagccgcg gcaagccgag aaatccgcag gtccccttga
     3061 tggtatggac gagccggaag atattatcga gaatcttcgc gttgttcgga tcctgctcga
     3121 aacgcacgag ctcgacgtcg accacgtcga gactctcgtt ggtctcggtg acaaattccc
     3181 gcagcaggtc gtccattaaa aaacgccttc gtcgacgggg ctggcccgtc cgccgctttc
     3241 cgtcggaagc gcggctagtt tgtcggtgaa gcgtttaatt ttggttgcgg ggccgcgaca
     3301 tcctgtctcc gttaaccggg cattaactgc cggaaacggc cgcggcagcg ctcactgcgc
     3361 ggtgatgacg accttctcgc cgtccatcgc gattgccgct ttgacgccac aggcctttgc
     3421 caggagcccg gcgtaaaatg gttggatgcg gtgagcgtcg agcccctcgg cggtctctcc
     3481 tccgagcaac acagatacag caggcggaac ctttgcattg atgccagcgg ccgtgacctt
     3541 gaaccccatc gtctcccccg atccgatggg atcgacggtc agcgcgccgc cgcgggggat
     3601 tgtctgtccc ccgatcgcca gcatgttgag aaggagtttg acccggttct tctgcaacag
     3661 cgcccgcgga aggttccagg caagctttgt cttttcgtcc tccaggaatc cgcgtgaaat
     3721 c
//
LOCUS       DASSRL010000272         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1599404, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000272 DASSRL010000000
VERSION     DASSRL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599404"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFL49_05590"
     CDS             <1..250
                     /locus_tag="VFL49_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7911480.1"
                     /translation="PCMQFLADILASPVDRPTVAETTAMGAAYLAGRAAGVCPDLSGF
                     AKQWRLDRRFEPRMDASERERKYAGWKQAVRRTLTAAN"
     gene            324..1427
                     /locus_tag="VFL49_05595"
     CDS             324..1427
                     /locus_tag="VFL49_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911481.1"
                     /translation="MAAHGKNRIAGERVPLIRFEAVSKRFGAVTAVETLSLDIAQGEF
                     FALLGPSGCGKTTLLRMLAGFETPSEGRIFLGGEDLVPVPPHRRPVNMMFQSYALFPH
                     LTVEGNIAFGLRQENVGREESAARVAEMLNLTRLQGFGGRKIHQLSGGQRQRVALARS
                     LIKRPRVLLLDEPLAALDKKLRAETQFELMELQRKLGTTFVIVTHDQEEAMIVADRIA
                     VMNQGRLMQVATPAEIYESPASRWVADFIGEVTIIEGRTAAPGRLDSALGPLSITDHG
                     PGAGQTVWLALRPEKIGMSAAPPTGEKNAVAGTIFEIGYRGEMSTYKVCLADRSLMKV
                     SIANVNARGRVPYAEGDAVWLSWPADAGILLAS"
     gene            1431..2351
                     /locus_tag="VFL49_05600"
     CDS             1431..2351
                     /locus_tag="VFL49_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7911482.1"
                     /translation="MQDGQEARGWGTRIVILLPYMWLAIFFLAPFVIVLKISLSQSVL
                     AQPPYTPVLDLATGWQGLKAFAAQLSLDSFRLLGSDSLYLLSYFKSLQVAAISTAFLF
                     ALGYPFAYGMARAPKAWQPLLVMAVVLPFWTSFLIRIYAWINILQRDGLLNDVLMRLH
                     IIDAPAAWLSSDTAIYIGIVYSYLPFMVLPLYASLERMDRSLLEAAADLGCPAWKAFW
                     LITFPLSLPGVAAGALLSFIPIVGEFVIPDLLGGSESQMIGQTLWTEFFSNRDWPVAS
                     AIAIALLGLLLVPILFYERMQQRSLEGGRT"
     gene            2348..>2854
                     /locus_tag="VFL49_05605"
     CDS             2348..>2854
                     /locus_tag="VFL49_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HET7911483.1"
                     /translation="MNRRSSSFNVVSVALGLAFLYLPIVILVIYSFNASRLVTVWGGW
                     SLVWYRELLNDEAMLSAAWISFRVALLSASAATVLGTLAAVSLTRMGRFRGRLLFSGM
                     VYAPLVMPEVITGLSLLLLFVALNLDRGFWTVVIAHTTLTTCFVTVIVQSRLIGFDRS
                     LEEAAMDLG"
BASE COUNT          470 a          876 c          899 g          609 t
ORIGIN      
        1 cccgtgcatg cagttcctcg ccgatattct ggcatctccg gtcgatcgac cgacggtcgc
       61 cgaaacgacc gccatgggcg ctgcgtatct ggccggacgg gcggccggcg tctgccccga
      121 tctttccgga tttgcgaaac agtggcggct cgatcgccgc ttcgagccgc gaatggacgc
      181 aagcgaacgc gagcggaaat atgcaggctg gaaacaggcg gtgcgacgga ccttgaccgc
      241 ggcaaactga catccggaca acgtcgtcgg gttgacgtgt gcgcgcgcgc cgttagcgtg
      301 tgcgccgcga aaacgggaaa ccgatggcgg ctcacggcaa aaatcggata gcaggcgaga
      361 gagtgccact gatccgcttc gaagcggtca gcaagcgctt cggcgccgtc acggcggtcg
      421 aaacgctcag cctggatatc gctcagggtg aattcttcgc gctgctggga ccgtccggct
      481 gcggcaaaac gacgttgctg cgcatgctcg ccgggttcga gacgccgagc gaaggccgta
      541 tcttcctcgg cggggaagac ctcgttccgg tgccgccgca ccggcggccc gtgaacatga
      601 tgttccagag ctacgcgttg tttccgcatc tcacagtcga aggcaacatt gctttcgggc
      661 tgcgccagga gaatgtcggg cgcgaggaaa gcgctgcgcg cgtggccgag atgctcaacc
      721 tcacccgcct gcagggcttc ggcggacgta aaattcacca gctgtcgggt ggccagcgcc
      781 agcgcgtggc gcttgcccgt tccctgatca agcggccgcg cgtgctcctg ctcgacgagc
      841 cgcttgcggc gctcgacaag aaactgcgcg ccgagacgca gttcgagctg atggaattgc
      901 agcgcaagct cggcaccaca ttcgtcatcg tcacgcacga tcaggaagag gcgatgattg
      961 tcgctgaccg catcgcagtg atgaatcaag gccgtctgat gcaggtagcg acacccgccg
     1021 agatctacga gagcccggct tcgcgctggg ttgccgattt catcggcgaa gtcacgatca
     1081 tcgaagggcg gacggccgcg cccggcaggc tcgacagcgc tttggggccc ttgtcgatca
     1141 ccgaccacgg cccgggcgcc ggacaaaccg tatggctggc gctgcgtccg gaaaaaatcg
     1201 gcatgagcgc ggcgccgccg acgggtgaga agaatgccgt cgccggcacg atattcgaga
     1261 tcggctaccg cggggaaatg tcgacctaca aggtttgtct cgctgaccgg tccctgatga
     1321 aagtctcaat tgccaacgtc aatgcgcgcg gccgcgtgcc gtatgccgaa ggcgatgccg
     1381 tatggctgtc gtggccggcg gatgccggaa tccttctcgc gagctagccg atgcaggacg
     1441 gccaggaagc acgaggctgg ggaacgcgga tcgtaattct gctgccatac atgtggctgg
     1501 cgattttctt tctcgcgccg ttcgtcatcg tgctcaagat cagcctgtcg cagtcggtgc
     1561 ttgcgcagcc gccttacacg cccgtgctcg acctggcgac gggttggcag ggacttaagg
     1621 cattcgcggc gcagctctct ttggattcct tcagattgct cggttccgat tccctgtacc
     1681 tgctgtccta cttcaaaagc ttgcaggttg ccgcaatttc taccgcattt ctgttcgcgc
     1741 tcggctatcc attcgcgtat gggatggccc gcgcaccgaa ggcctggcag cccctgctgg
     1801 tcatggccgt cgtgctgcca ttctggacgt cgttcctgat ccgcatctat gcgtggatca
     1861 atattctgca gcgtgacggg ctgctcaacg acgtcctcat gcggttgcac atcatcgacg
     1921 ctcccgccgc gtggctgtcc tccgacaccg cgatctatat cggtattgtc tattcctatc
     1981 ttccgttcat ggttctgccg ctctacgcaa gcctcgaaag gatggatcgg tcattgctgg
     2041 aagcggcggc cgaccttggc tgcccggcgt ggaaggcgtt ctggctgatt acctttccgc
     2101 tgtcgcttcc gggcgtcgcc gcgggggcgc tgctgtcgtt cattcccatc gtcggcgaat
     2161 tcgtcattcc ggacctgctc ggcggttcgg aatcgcagat gatcggccag acactgtgga
     2221 ccgagttctt ctccaatcgc gactggcctg tcgcttcggc tattgcgatc gcactcctcg
     2281 gcctcctgct ggtgccgatc ctcttctatg agcgtatgca gcagcgctcg ctcgaaggag
     2341 gacggacatg aaccgccgat cctcttcgtt taacgttgtc tcggtcgcgt tggggctggc
     2401 gtttctgtat ctgccgatcg tcatcctcgt gatctattcg ttcaatgcat cgcggctggt
     2461 cacggtctgg ggcggctggt cgctcgtatg gtaccgcgag ctgctgaacg atgaggcgat
     2521 gctcagtgcg gcctggatca gcttccgtgt cgccctgctg tcggcaagcg ccgcgacagt
     2581 gctcggcaca ctggcggccg tttccctcac ccgcatggga agatttcgcg gccggctgct
     2641 tttttccggt atggtctatg ccccgcttgt gatgcccgaa gtcattaccg gattgtcgct
     2701 gctgctgctg tttgtcgcgc tcaacctgga ccgcggtttt tggacggtcg ttattgctca
     2761 tacgacgctg acaacgtgct tcgtgaccgt gatcgtgcaa tcgcggctga ttggatttga
     2821 ccggagcctc gaggaggccg cgatggacct cggg
//
LOCUS       DASSRL010000273         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1442199, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000273 DASSRL010000000
VERSION     DASSRL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442199"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFL49_05610"
     CDS             complement(<1..192)
                     /locus_tag="VFL49_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HET7911484.1"
                     /translation="MRVDISFLPALAAAFLLAFARVGAMVMLLPGVGEQTMPSRVRLG
                     IALVLTAVILPAHQKAYTVD"
     gene            complement(198..461)
                     /locus_tag="VFL49_05615"
     CDS             complement(198..461)
                     /locus_tag="VFL49_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HET7911485.1"
                     /translation="MTGPEVLDVSRDAIVTLIVVSSPVMLVGLAVGVAISLLQALTQI
                     QEMTIAFVPKILAIFLSLLIALPFMAEKLHAEMLRIAARIVSG"
     gene            complement(474..797)
                     /locus_tag="VFL49_05620"
     CDS             complement(474..797)
                     /locus_tag="VFL49_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HET7911486.1"
                     /translation="MASPLAAASAYASVARIASDASSLSVIRQPVEAKGETSFASMLK
                     DAVGSVAEMGRKSDAQARAAVNGKGNLVDVVTAVSETEVAIDAVVAVRDRVIQAYEDI
                     MKMPI"
     gene            complement(812..1222)
                     /gene="flgC"
                     /locus_tag="VFL49_05625"
     CDS             complement(812..1222)
                     /gene="flgC"
                     /locus_tag="VFL49_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HET7911487.1"
                     /translation="MDFIKTLTIAASGLRAQAGRMRIISENIANADSTPQRPGADPYR
                     RKIPTFRSELDRTLDTQLVELGKVKTDTSAFRLKYEPGHPAADSNGNVKYPNVNSLIE
                     MTDMREAQRSYEANINVISATRRMIQRTIDILKT"
     gene            complement(1253..1663)
                     /gene="flgB"
                     /locus_tag="VFL49_05630"
     CDS             complement(1253..1663)
                     /gene="flgB"
                     /locus_tag="VFL49_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HET7911488.1"
                     /translation="MAITDIPILSMLRTRLDWSQARQRVLAENVANADTPNFRARDLA
                     PPKFDDPPAAASTTVAGISLTRTASGHIEGVGLSQSPFANGAARVYEVRPAGNAVNLE
                     EEMMKVAANQMDFQTATALYTRSLNLIKTALGKR"
     gene            1937..>2470
                     /locus_tag="VFL49_05635"
     CDS             1937..>2470
                     /locus_tag="VFL49_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET7911489.1"
                     /translation="MFESLFGAEMPLAARFFIAFLVVLALIGLTAWLVRRFGANRLGN
                     AARGRQPRLAVIDAATVDGRRRLVLIRRDNIEHLLMIGGPTDVVIEPNIVRAVAARDA
                     VREQARVASAGDGRQPPTIENGWPLQPTPEPAPAARPYRPSATDEPWLAPEPGARPRP
                     ADSLPGLAGELSARLNPS"
BASE COUNT          458 a          738 c          782 g          492 t
ORIGIN      
        1 atcgaccgtg taggccttct gatgtgcggg taatatgacc gcggtgagca cgagcgcgat
       61 gccgagccgg acgcgcgatg gcatcgtctg ctcgccgacg cctggcaaca gcatcaccat
      121 ggcgccgacg cgggcgaagg cgagcagaaa tgcagcagca agcgccggta aaaatgagat
      181 atcgacccgc atcaggccta accgctgacg atgcgtgcgg cgatccgcag catctcggca
      241 tgcagcttct cggccatgaa tggcagcgcg atcagcaacg acagaaaaat ggccaggatc
      301 ttcggcacga aggcgatagt catttcctgg atttgtgtaa gcgcctgcag gagggaaatg
      361 gcaacgccga cggcaagccc gaccagcatt acggggcttg agacgacgat cagcgtcacg
      421 atggcgtcgc gcgatacgtc gaggacttcg ggaccggtca tgtcatctgc tgcctaaatc
      481 ggcatcttca tgatgtcttc gtacgcctga atcacacggt cgcgcacggc gacgacggca
      541 tcgattgcga cctcggtctc ggaaacggct gtgacaacgt cgacaagatt cccttttcca
      601 ttcacggcgg cgcgcgcctg ggcgtcggac ttccgcccca tctcggcgac cgagccaacc
      661 gcatctttaa gcatggaggc gaagctcgtt tcgcctttcg cctcgacggg ctgccggatg
      721 acggacaatg acgatgcatc ggatgcgata cgcgcgacac tggcataggc gcttgcggca
      781 gcgagcggag aagccatcgt gatggtgcct ttcaggtttt gagaatatcg atcgtgcgct
      841 ggatcatgcg ccgggtcgcg ctgataacgt tgatattcgc ctcgtatgac cgttgcgctt
      901 cgcgcatgtc cgtcatttcg atcagcgaat tgacgttggg atatttgacg ttgccgttgc
      961 tgtcggcggc ggggtgaccg ggctcgtatt tgaggcggaa ggcggatgta tcagtcttca
     1021 ccttgccaag ttcgacgagc tgggtatcaa gcgtgcgatc cagctcgctg cgaaatgtcg
     1081 gaatcttgcg gcggtaggga tcggcacccg gccgctgcgg cgtcgaatcc gcgttcgcga
     1141 tgttctctga aataatgcgc atacgccccg cctgcgcgcg caggccggat gcggcgatcg
     1201 tcagcgtttt aatgaaatcc aaagcgtttc tccccgacgg aggtgggcgc gcctagcgtt
     1261 tgccgagcgc cgtcttgatg agattgaggc tgcgcgtgta gagcgccgtt gccgtctgaa
     1321 aatccatctg gttggcggcg accttcatca tctcctcttc gagattcacg gcgttgccgg
     1381 cgggacgcac ctcgtaaacg cgggccgctc catttgcgaa aggcgactgg ctcaagccga
     1441 ctccctcgat atggccactg gccgtacgcg tgagcgaaat gccggccacc gtcgtcgacg
     1501 cggctgccgg cggatcgtcg aacttcggtg gggcgaggtc gcgcgcccga aaattcggcg
     1561 tgtcagcatt tgcaacgttt tccgcaagca cacgctggcg ggcctgtgac cagtccagcc
     1621 gggtccgcag catcgacagg ataggaatat ccgtgatcgc catagccctc ttccccaaag
     1681 cgccccccag cggcaggcgg ccgcgctagg cagactttgc cgggtgtatg gttaacgggc
     1741 ggtaaaagga gacggcaggc cggttgtggt ttggccgttg tcgctgcaaa tggccagatt
     1801 cagagcctac ccgtaaattg ccactttatt tgttaaacct ctgttagtat tcagggcggc
     1861 aaatagtgcc tagcgaaatt aaccattcgg gcgattctcg cccgacgcgc gttggcggga
     1921 agaacggagc gcattgatgt tcgagagctt gttcggcgct gagatgccgc tggccgcccg
     1981 cttcttcatc gcctttctgg tggtgctggc gcttattggt ctgacggcgt ggctggtacg
     2041 acgtttcgga gcgaaccgtc tcgggaacgc cgcgcgcgga cgccaacccc ggcttgcggt
     2101 gatcgacgcc gcaacggttg atggacggcg acgcctggtt ttgattcgcc gcgacaatat
     2161 cgaacacctt ctgatgatcg gtggaccgac ggatgtcgtt atcgagccga atattgtgcg
     2221 cgccgttgca gcgcgcgacg cggtacgcga gcaggcacgt gttgcttcgg ccggcgacgg
     2281 acgtcagccg ccaacgatcg aaaatggctg gccattgcaa ccgacgcctg agcccgcgcc
     2341 tgcggcacgc ccctatcggc cttccgccac agacgagccg tggcttgcgc ccgaacccgg
     2401 cgctcggcca cgccctgcag acagcctccc cggcctcgcc ggcgaattgt cggcgcgtct
     2461 caatccttcc
//
LOCUS       DASSRL010000274         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_321881, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000274 DASSRL010000000
VERSION     DASSRL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321881"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFL49_05640"
     CDS             complement(<1..209)
                     /locus_tag="VFL49_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135090.1"
                     /note="cytochrome c-type biogenesis protein; required for
                     the transfer of heme to apocytochrome c; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HET7911490.1"
                     /translation="MIPEVGHFALVLALGLGLIQSTVPVFGAHKRDGALMRLAGATAI
                     AQFVFVASSFLALTMCYVTSDFSVAT"
     gene            complement(206..670)
                     /gene="ccmE"
                     /locus_tag="VFL49_05645"
     CDS             complement(206..670)
                     /gene="ccmE"
                     /locus_tag="VFL49_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET7911491.1"
                     /translation="MTRKQRRLTLIGTSLAVLAVAAALVLSALRDSIVFFSSPTDVAE
                     RHAAPGTRMRIGGLVKPGSIERGDNLLVRFAVTDGKTDIAVRYQGIVPDLFREGQGVV
                     AEGKLDTDGIFTADTVLAKHDERYMPREVVDALKKSGHWQEGEQKGSGSATK"
     gene            complement(677..1798)
                     /gene="ccmI"
                     /locus_tag="VFL49_05650"
     CDS             complement(677..1798)
                     /gene="ccmI"
                     /locus_tag="VFL49_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET7911492.1"
                     /translation="MTLWFVFALMTAAAIFAVLWPLGQVANPQTGNDLAVYRDQLDEI
                     ERDQAAGLIGAAEAEAARTEVSRRLLAAADAAELAHALPAGSPLWRRRAVTAGAIVLL
                     PSIAAAFYLALGSPQLPGQPLAERARQAHQGRSIASLVSQAEAHLERNPDDIRGYEVL
                     APIYLRMGRFADAVSARRKLLTLGGDNAGRQSDLGEALVAAANGVVTADAKSAFERAL
                     ALDPDELKAKFYVGLAAEQDGDRARAAEIWGAMTKNAPADAPWLPMVREALARVGASP
                     PAGTTPGPTADDIAAAGAMSEKDRGEMIRTMVARLADRLKENGTDVEGWQRLLRAYLV
                     LGERDKAMSAAGAAKRALADDADKLRRIEDTIKSLGLEG"
     gene            complement(1920..>2686)
                     /locus_tag="VFL49_05655"
     CDS             complement(1920..>2686)
                     /locus_tag="VFL49_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911493.1"
                     /translation="IESAAARDGELFLYALLTVMNRMAAPWQLIRLGVKAAGSDTAAR
                     VADTHYGVAVNIVLAELERMVAELREDLRSGQGVAVGALLKSIHDSTRGLRTELALPV
                     DSTWGRALAAQRTHISDILRAEIESMPSRVRHLLRPRPAKEIRPNSVLDPNEVAEVEA
                     LVEFVGICRYFAGELAINEMTQRALSELRHFLDNGTQALLEGLRHAGDADRSFRQSQV
                     DAAVRFCAKVFGREYAALLGKAAEVAVIGDSRAARA"
BASE COUNT          534 a          872 c          783 g          497 t
ORIGIN      
        1 gttgcgacgg aaaagtccga cgtcacgtaa cacatcgtga gcgcaagaaa cgacgaagca
       61 acaaaaacaa actgagcaat tgcggtcgcg ccggcaaggc gcataagcgc gccgtcacgc
      121 ttatgcgcgc caaagactgg caccgtcgat tggatcagcc ccaagcccag cgccaatacc
      181 aatgcaaaat gaccaacctc cggaatcatt tcgttgcact cccggagccc ttttgctcgc
      241 cttcctgcca gtgtccggat tttttcagcg cgtcaacaac ctcgcgcggc atgtagcgtt
      301 catcgtgttt ggcgagaaca gtatcggctg taaatattcc atcggtgtca agtttgccct
      361 cggcaacaac cccctgcccc tcgcgaaaca agtcagggac gatgccttga taccgcaccg
      421 caatgtcggt cttgccgtcg gtcacggcga atcgcacgag caggttgtcg ccgcgctcta
      481 tactcccggg tttgacgaga ccgccgatgc gcatgcgggt gcccggcgct gcgtgccttt
      541 cggcgacatc ggtcggcgaa ctgaaaaaaa cgatcgagtc gcgcaacgcg ctcagcacga
      601 gcgccgcagc aaccgccaga acggccaggc tggtgccaat cagagtcaga cgtcgttgtt
      661 tgcgtgtcat tccttctcag ccttcgagcc cgaggctttt gattgtgtcc tcaatacgac
      721 ggagcttgtc ggcatcgtcg gcgagagctc gcttcgcagc gccggcggca gacatcgcct
      781 tgtctcgctc cccgagaacc aaataggcac gcaagaggcg ttgccatcct tcgacatccg
      841 ttccattttc tttcaaccgg tcggcaaggc gagcgaccat agtgcggatc atttcgccgc
      901 gatctttctc gctcatggcg ccggcggcgg caatatcatc ggcggtcggc cccggcgtag
      961 tgccggccgg tggtgacgca ccgacgcgtg ccaaagcctc ccgcaccatc ggaagccacg
     1021 gcgcgtcggc cggcgcattc ttagtcattg ccccccatat ctcggccgct ctggctcggt
     1081 cgccatcctg ctcagccgcg aggccgacgt aaaatttggc cttaagttcg tctggatcga
     1141 gcgccagcgc acgttcgaac gcggatttgg catccgcagt aacgacgcca ttggccgctg
     1201 caacgagtgc ctcgccaaga tccgattgcc ggccggcgtt atcgccgccg agcgtcagta
     1261 acttgcgccg tgcgctcacg gcatcggcga agcggcccat ccgaagatag atcggcgcca
     1321 acacttcgta gccgcggatg tcgtccggat tgcgttcaag atgtgcttcg gcctgcgaga
     1381 cgagacttgc aattgaacgg ccttgatggg cctgacgtgc ccgctctgcc agaggctggc
     1441 ctggcagctg cggcgatccg agggcaaggt aaaatgcagc agcgatgctc ggcaataaaa
     1501 cgatagcgcc ggccgtcacc gcacggcggc gccaaagcgg cgacccggcc ggcaaggcgt
     1561 gagcgagttc cgcggcatcg gcggccgcga ggagtcggcg cgagacctcg gtgcgagccg
     1621 cttctgcttc cgccgccccg atcagtccgg cggcctgatc acgctcgatt tcgtccagct
     1681 ggtcacgata gaccgcaaga tcgttcccgg tctgcggatt cgcgacttgg ccaagcggcc
     1741 acaagacggc gaaaatcgcc gccgctgtca tcagcgcaaa cacaaaccaa agggtcatgg
     1801 agccttaaat tccgcgcgcg ccctccacca gaggacggca ccgcagggaa tacattacgt
     1861 ccggtcatga cggcaatgtg cattatcgta caaggaacct tgcggcaaaa gtggcccttt
     1921 taggcgcggg ctgccctgct gtcgccaata actgcaactt ccgcggcctt gcccagcagc
     1981 gcggcatatt cccgtccgaa aactttggcg cagaaccgca cggccgcatc gacctgtgac
     2041 tgccggaagc tgcgatcggc gtcgcccgcg tggcgcagtc cttcaagcag tgcttgcgtg
     2101 ccgttgtcga gaaaatgccg caactcgctc agggcgcgtt gcgtcatctc attgatcgcg
     2161 agctcgccgg caaagtaccg gcatattccg acaaactcga ccagcgcctc aacctcggca
     2221 acctcattgg gatcgagtac ggaatttggc cgaatttctt ttgccggacg tggccgcaac
     2281 agatgccgga cccgactcgg catggattcg atctctgctc gcagaatatc ggaaatgtgt
     2341 gtgcgctggg cagccagcgc gcggccccag gtgctgtcaa ccggaagcgc gagctcggta
     2401 cgcagaccgc gtgttgagtc atgaattgac ttgagcagcg cacccacagc aacaccttgc
     2461 ccgcttcgca ggtcctcacg caactcagcg accatgcgtt cgagctcggc caggacgata
     2521 ttgaccgcga cgccatagtg cgtgtcggcg acgcgcgccg ccgtatcgct tccagccgcc
     2581 ttcactccaa gccggatcag ttgccagggt gcagccatcc ggttcatgac cgtcagcaag
     2641 gcataaagga agagttctcc gtcgcgtgcc gccgcacttt cgatga
//
LOCUS       DASSRL010000275         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1232185, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000275 DASSRL010000000
VERSION     DASSRL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232185"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..215)
                     /locus_tag="VFL49_05660"
     CDS             complement(78..215)
                     /locus_tag="VFL49_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911494.1"
                     /translation="MVGKIARGAYVFVVATMFVAWIMSFNSEEPAKPAQAGPQIWYIY
                     S"
     gene            400..1359
                     /locus_tag="VFL49_05665"
     CDS             400..1359
                     /locus_tag="VFL49_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HET7911495.1"
                     /translation="MAGPVIAVTDSVFPSLDPAKAALSGLNPTYRMAKSANADDILAV
                     AKDADAILVTYAKLSREVLTQLTRCRAIGRFGLGVDNIDLVAAKEKGIAVNYVPDYCI
                     REVSDHAMALLLGLIRKIPLSNKLVQGGRWEMPAVVPIRRIEGTVLGLVGFGHIPRLI
                     APKAQAFGMKVLAYDPFAKPDVFKAARVESVDFDTLLNRSDYVSVHAPLTAETRGMMN
                     AAVFAKMKKGAYIINTARGPLIDETALVAALDSGQVGGAGLDVVAAEPLAKDSPLLGR
                     DNVIISPHTAFYSIEALEELQTKCASDVARVLSGEKAIYPISA"
     gene            1363..1869
                     /locus_tag="VFL49_05670"
     CDS             1363..1869
                     /locus_tag="VFL49_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7911496.1"
                     /translation="MLDRTDLDPVFFAPFVSSLMHVEPQWIDYNGHLNMAYYNVLFDR
                     AVDEVYELIGIGGDYLKRANHSTFTAEAHVRYVRELHVDDPVRVTFQLLGFDSKRFHY
                     FEQLFHATEGWVSATSENMTLHVNMATRKVTHFPDTIMRSLARMKAAHAKLPRPEAAG
                     RHIAMPGR"
     gene            complement(1874..2041)
                     /locus_tag="VFL49_05675"
     CDS             complement(1874..2041)
                     /locus_tag="VFL49_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET7911497.1"
                     /translation="MLGWVVTFLIIGLIAAILGFGGIAGASFAAAKIIFFVAIILLLL
                     SIVFGAIRRPL"
BASE COUNT          467 a          696 c          633 g          452 t
ORIGIN      
        1 gggaattacg acctgcgcca atgcggaatt catgtgcgtc gaccgtaagc agagcccgcc
       61 gccaccggac aatagactta cgaatagata taccagatct gaggaccggc ctgcgccggc
      121 ttcgccggtt cttcgctatt gaacgacatg atccaggcga cgaacatcgt ggcgaccacg
      181 aacacgtagg ctcctcgagc aatctttcca accattgccg cgctcccgta tttttttgtc
      241 aggatagtcg acagggcccg ccgagcgtgg ccgtcgttaa tttttggtta atcgcgctaa
      301 gccattggaa tcgtgggcgg ccctcggccg cgcgggtgga cgcccgcaca ctgctgtcgt
      361 agcttcgcga cagttccgat ccttttcgtg gagtttgtaa tggccggccc cgtgattgca
      421 gtgaccgaca gcgtgtttcc gtccctcgac ccggccaagg ccgcgctttc cgggctcaac
      481 cccacctacc gcatggcgaa aagcgccaac gctgacgaca tcctggcggt ggcgaaagat
      541 gccgacgcta ttctcgtcac ctatgcgaaa ttgagtcgcg aggttctcac gcaattgact
      601 cgctgccgcg cgatcggccg cttcggtctc ggcgtcgaca acatcgatct cgtcgccgcc
      661 aaggaaaaag gcatcgccgt caactacgtg cccgactatt gcatccgcga ggtctccgac
      721 catgcgatgg cgctgctgct cgggctgatc cgaaagattc cgttgtcaaa caagctggtg
      781 cagggcggac gttgggaaat gccggccgtc gtcccgatcc gccgtatcga aggcacggtg
      841 cttggtcttg tcggctttgg ccacatccct cgcctgattg cgccgaaggc ccaggccttc
      901 ggtatgaaag tgcttgctta tgatcctttc gcaaagccgg acgtgttcaa ggctgcgcgt
      961 gtagaaagcg tggacttcga cacccttctc aacagatccg actatgtctc tgtgcacgcg
     1021 ccgctcacgg cggagacgcg cggcatgatg aacgccgccg tcttcgcgaa gatgaaaaag
     1081 ggcgcctata tcatcaacac ggcgcgcggc ccgttgatcg acgagacggc gctggttgcg
     1141 gcgcttgatt ccggacaggt tggcggtgcc ggactcgacg tggtggcagc cgagccgctg
     1201 gccaaagact cgcctctgct tggccgcgac aacgtcatca tctctccgca tactgcgttc
     1261 tattcgatag aagcgctcga agaattgcag accaaatgcg ccagtgacgt cgcgcgcgtc
     1321 ctcagcggcg aaaaggcgat ttatccgatc tcggcctgaa gcatgctcga tcgcaccgac
     1381 ctcgacccgg ttttcttcgc accgttcgtc tcgtccttga tgcatgtcga gccgcagtgg
     1441 atcgactaca acggccacct caatatggct tactacaacg tgcttttcga tcgcgccgtc
     1501 gatgaagtct acgagctcat cggcatcggg ggcgattacc tcaagcgagc caaccattcg
     1561 accttcaccg ccgaggcgca tgtgcgctac gtacgcgaat tgcacgtgga tgatcccgtg
     1621 cgcgtgacct tccagcttct cggcttcgat tcgaagcgat tccactattt cgagcagctg
     1681 tttcacgcga ccgagggctg ggtgtccgcc acttcggaga acatgacgct gcatgtgaac
     1741 atggcgacca ggaaggtcac ccattttccc gacacgatca tgcgctcact tgcgcgcatg
     1801 aaggccgcgc acgcaaaatt gccacggccg gaggctgccg gacgccacat cgcaatgccc
     1861 gggcgatgat ccgttaaagc ggccgtctga tcgctccgaa cacgatcgac agaagcaaca
     1921 ggatgattgc gacgaagaaa atgatcttcg cagccgcaaa cgatgcgccg gcgataccgc
     1981 caaatccgag gattgccgca atcagcccga tgatgagaaa agtcacgacc cagccgagca
     2041 tgatcgactc ctgaatccct tccccgtcga caacagcacg gacaatcgaa aagttcctgc
     2101 cggccggaac tatcgcaatt ttgccttgtt agattgtcga cagcggcacg ctgaaagcgc
     2161 agccgagcat ttttcaggag acggccatgg ccgacactgc aatcgttggc gaaaatgtcg
     2221 aggtaattca accgcgcggt gcccgcac
//
LOCUS       DASSRL010000276         6184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_619818, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000276 DASSRL010000000
VERSION     DASSRL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619818"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="pgeF"
                     /locus_tag="VFL49_05680"
     CDS             <1..540
                     /gene="pgeF"
                     /locus_tag="VFL49_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET7911498.1"
                     /translation="AKEPWTRDGRPRADAVVTHLPRLAIGVSTADCGPLLFADGEAGV
                     IGAAHAGWRGALSGVVEATVAAMEQLGAKHGRITAALGPTIRQSNYEVGPEFMEQFVA
                     ADPANKRYFAPSQREGHAMFDLSGYIARRVERAGVAQFEDLGLCTYEEPGRFYSYRRS
                     THRGEPDYGRHINAIALSA"
     gene            639..1376
                     /locus_tag="VFL49_05685"
     CDS             639..1376
                     /locus_tag="VFL49_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911499.1"
                     /translation="MSMELSWGHRARGAPRRVWRVGALLAAALALAACNPDNQGLSAQ
                     QPRGATVAFDSLDGLPPSQFQRLVKNLNDEAQSRRLAVISRERPSAYHVRGYLAARVT
                     KHQTTVSWVWDVFDQDEHRALRISGEETAKGHHKDAWAAADDAMLGRIAQSSMDQLAA
                     FLTSPEVAPGIAPPPEPQIALAGTSDSSPEAAGIFRIFRPQADPISTASAEQPGEDEA
                     GPVPLPRRRPPALVSSRETLMLAASRR"
     gene            1493..2446
                     /locus_tag="VFL49_05690"
     CDS             1493..2446
                     /locus_tag="VFL49_05690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028174674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7911500.1"
                     /translation="MSGKNGAIKLVAGNSNPALARGISDHLNLPLTKAVVRRFADMEI
                     FVEIQENVRGADVFVIQSTSFPANDHLMELLIIIDAMRRSSARRITAVIPYFGYARQD
                     RKPGPRTPISAKLVANLITRAGADRVMTLDLHAGQIQGFFDIPTDNLYASPVMVRDIR
                     ERFKLDNVMVVSPDVGGVVRARGLAKRINAPLAIIDKRRERAGESEVMNVIGDVAGYT
                     CVLVDDIVDSGGTLINAADALLESKAKAVYAYISHGVLSGEAAKRISGSRLKELVITD
                     SIQPTKEILAAGNIRVLEIAPLIGEAIGRTAAEESVSSLFD"
     gene            2748..3248
                     /locus_tag="VFL49_05695"
     CDS             2748..3248
                     /locus_tag="VFL49_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HET7911501.1"
                     /translation="MSLTSYAEEPRINPLDVVERLATGNDWSFERASDDEITILVTGR
                     WADYQLSYTWMGDIEALHLACAFDLRVPERRCAEVQALITLINERLWVGHFDHWVTEG
                     IVMFRHALVLAGGTEASGKQCEALLGTALDSCERYFTAFQFVVWAGKTAREALDATMF
                     ETSGEA"
     gene            3277..4119
                     /gene="proC"
                     /locus_tag="VFL49_05700"
     CDS             3277..4119
                     /gene="proC"
                     /locus_tag="VFL49_05700"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7911502.1"
                     /translation="MTRAKAADKPLRGLSGRLVLLGAGKMGSAMLEGWLARGLDPRNL
                     AVIEPHPVKAIRALTRRGVTINPRKKISGVSAVVIAVKPQNAPEAIPALEPFVGASTL
                     ALSIMAGRTIVFLQGTLPQGTPIVRAMPNTPAAVGRSITVACANAMVNTRQRKLASDL
                     LSAIGKVEWANDETLMDAVTALSGSGPAYVFLLAEAMASAGIAAGLPAALATRLAHDT
                     VAGSGELLHRSQLSAATLRENVTSPGGTTAAALDVLLGPGGLEELMTKAIAAATRRSR
                     DLAG"
     gene            4190..4885
                     /locus_tag="VFL49_05705"
     CDS             4190..4885
                     /locus_tag="VFL49_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7911503.1"
                     /translation="MARKSKRPRRSPAAPPSRGGSDRDKAIDALMSLLGDRPFEEIGL
                     AEIAGRAGIKLSQLRAEFGSTLAILAAHIKEIDRAVLAGGDGDMAEEPARERLFDVLM
                     RRLEALAPYKDAIRSLMRSTRRHPGLAFALNAMAVRSQRWMLEAAGIGTSGPRGALRA
                     QGAALMFARVLSVWIDDEDEGLDRTMATLDRGLASAERWAGFLDDLCALPACVLRGPR
                     RRRRMRDEEAEAA"
     gene            complement(4882..>6184)
                     /locus_tag="VFL49_05710"
     CDS             complement(4882..>6184)
                     /locus_tag="VFL49_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7911504.1"
                     /translation="SVMPKGLYARALLIIIAPMVILQSVVAFVFMERHWNVVTQRLSA
                     SVVQDIAALIDIYRGYPQDADQAQIRRIAQQRLGLVVDFLPLSEMPPPGPKPFFSLLD
                     QALAEELRKQIGRPYWMDTVGKSALLEIRVQLDNTVMRVFARRSAAYVSNSEIFLLWM
                     VGTSTVLLTVAIIFLRNQIKPILRLADAAESFGKGREVPNFRPRGAREVRRAAAAFIE
                     MKTRIERSIEQRTAMLAGVSHDLRTVLTRFKLELALIKKKTPEVEAMQKDIDEMARML
                     EAYLAFARGDSGETAAPTDMAEFLEELRLDAERHGHKATVIFHGPPIVTVRPAAFKRC
                     LANLVSNAARHAASIAISGHRDHRYLTVTIDDDGPGIPAEMREEVFKPFLRLDDARNQ
                     DEGGTGLGLAIARDIARSHGGDITLGDTPMGGLRATVRVPV"
BASE COUNT         1103 a         1996 c         1977 g         1108 t
ORIGIN      
        1 gcaaaggagc cgtggacgcg cgacggccgg ccgcgcgccg acgcggttgt cacgcacttg
       61 ccacgcctgg cgatcggcgt gtcgacggcc gattgcgggc cgctgctgtt cgccgacggc
      121 gaggcgggcg tgatcggtgc agcgcatgcc ggatggcgcg gcgcgctgtc gggtgtggtc
      181 gaagccaccg ttgctgccat ggagcagctt ggtgcgaagc acggacgcat cactgccgcg
      241 ctcggcccaa ctatccggca atcgaactac gaagtcggcc ctgaatttat ggagcagttt
      301 gttgccgccg atccggcgaa caagcgctat ttcgctccct cgcagcgcga aggccatgcc
      361 atgttcgacc tctccggcta tattgcgcgg cgtgtcgagc gcgcgggcgt tgcacaattc
      421 gaggatcttg gcttgtgcac atatgaggag cccgggcgtt tctacagcta ccgccgcagc
      481 acgcatcgcg gcgagcccga ctatggccgc catatcaatg caatcgcgct gagcgcctag
      541 ttctcacgca atgagaggcg tctggactcc ggactgcggt tgccgttacg gttgaccccg
      601 gccggacggg tttaagagcc aaaaacaagg ccgcaggggt gtctatggag ctgagttggg
      661 gtcaccgcgc gcgcggcgcg ccgcggcgtg tctggcgtgt cggtgccttg ctcgcagcag
      721 cgctcgcact ggccgcatgc aatcccgaca atcagggcct ctccgcacaa cagccgcgcg
      781 gcgccaccgt tgcattcgat tccctcgacg gtttgccgcc gagtcaattc cagcgtctcg
      841 tgaaaaatct caacgacgag gctcagtcac gtcgccttgc ggtgatttcg cgcgaacgac
      901 cgtcggctta ccacgtgcgc ggctatctcg ccgcgcgggt gacaaagcac cagaccaccg
      961 tctcctgggt gtgggacgtc tttgatcagg acgagcaccg cgcattgcgc atcagcggcg
     1021 aggaaaccgc caagggccac cacaaggatg cctgggcggc cgctgacgac gccatgctgg
     1081 ggcggatcgc gcaatcgagc atggatcagc ttgccgcttt cctgacatcg ccggaggtcg
     1141 cgcccgggat cgcacccccg ccggagccgc aaattgccct cgccggcaca agcgattcct
     1201 cccccgaggc cgccggcatt ttccggattt tccgcccgca ggccgatcca atatcgacgg
     1261 cgagtgccga gcaacccggt gaagatgaag cgggaccggt gccgctcccg cggcggcgcc
     1321 cgccagcact cgtatcgtcg cgggagacgc tgatgcttgc ggcttcccgc cgctaagtcg
     1381 caattcatac tttcggccaa ggctggacaa caatttccag ccgtattgcc gcgccctgcc
     1441 cgccttgtta tgaccacgcc gcgcgtgacg acgtgagggc aaggatttcg ggatgtcggg
     1501 caagaacgga gcgatcaagc tcgtcgccgg caattccaat cccgcgctgg cgcgggggat
     1561 ttccgaccat ctcaacctgc cgctgaccaa ggccgtggtc cggcgtttcg ccgacatgga
     1621 gattttcgtc gagatccagg aaaacgtgcg cggcgccgac gtcttcgtga tccagtcgac
     1681 ctccttcccg gccaacgatc atctcatgga gcttctgatc atcattgacg cgatgcggcg
     1741 ctcgtccgcg cgccgcatca ctgcggtgat tccctatttc ggatatgcgc gacaggaccg
     1801 caaacccggc ccacgcacgc cgatctcggc gaaactggtc gccaacctga tcactcgcgc
     1861 aggcgcagac cgcgtgatga ccctcgacct gcatgccggc cagattcagg gtttcttcga
     1921 cattccgacc gacaacctct atgcgtcgcc ggtgatggtc cgcgacatcc gcgagcgctt
     1981 caagctcgac aatgtgatgg tcgtgtcgcc cgacgtcggc ggcgtggtgc gcgcgcgtgg
     2041 attggccaaa cgcataaacg cccctctcgc gatcatcgac aagcggcgcg agcgcgccgg
     2101 cgagtccgaa gtgatgaacg tgatcggcga cgtcgcggga tacacctgcg tgctcgtcga
     2161 tgacatcgtc gattccggcg gcacgctgat caacgccgca gacgcgcttc tcgaaagcaa
     2221 agccaaggcc gtctatgcct acatcagcca cggcgtgctt tccggtgaag cggcaaagcg
     2281 catctcggga tcacgactta aggaactggt gatcacggac tcgatccagc cgacaaaaga
     2341 aatcctcgcc gccggcaata tccgagtact ggaaatcgcg ccgctcatcg gcgaggcgat
     2401 cggacgcacc gcggccgagg agtcagtgtc tagtctgttt gactgaacgc accgcgcgat
     2461 tgacttatcc accgttgagt cacgttgcca cagcattggc tgtggagagt cgaatcggct
     2521 cggttgcctc taacaggcaa atcacggctg attggcactt gacgatgcct ttggcgggcg
     2581 gaatcaggcg cgatatagtc gaatcgcttg gtgattcgct ttcgccgtca aggcatcccg
     2641 taacccctgc gtctaacggc ggtccgggcc gcgtctcggg tcatccccgt gtcgtgcgtg
     2701 ttgtggcgtg cgtttcctcg cgctccaatc ggcaaagcgg agacgtcatg tccctgacaa
     2761 gctacgcgga agagccgcgc atcaatcccc tcgacgtggt ggagcggctc gcgaccggca
     2821 atgactggtc tttcgaacgc gccagcgacg atgaaattac gatcctggtt acgggacgct
     2881 gggctgacta tcagctctct tacacctgga tgggcgacat cgaagcgctg catctggcat
     2941 gcgcctttga cctgcgagta ccggaaaggc gatgcgcgga ggtgcaggcc ctgatcaccc
     3001 tgatcaacga acggctttgg gtcggccact ttgaccattg ggtcaccgag ggcatcgtca
     3061 tgttccgcca cgccctcgtc ctcgcgggcg gcacagaggc ctcgggcaag cagtgcgagg
     3121 cgctgctcgg cacggcgctc gactcctgcg agcgctattt cacggccttc caattcgtgg
     3181 tctgggccgg caagaccgcg cgcgaagcgc tcgacgcgac gatgttcgaa acatccggtg
     3241 aggcatgacg gcgcaaatgg cgatataatc gtcgccatga cgcgggcaaa agctgcggac
     3301 aagccgttgc gtggactttc cggccgcctt gttctgctcg gcgccggcaa gatgggctcg
     3361 gccatgctgg agggctggct cgcgcgcgga cttgatccgc gcaacctcgc cgtcatcgag
     3421 ccgcatccag tcaaggccat cagggcactg acgcggcgtg gcgtgacgat caacccccgc
     3481 aaaaaaattt ccggagtcag cgcggtcgtg atcgcggtaa agccgcagaa cgcgcctgaa
     3541 gcgataccgg cgcttgagcc ttttgtcggt gcgtcgacgc tggccctttc gatcatggcg
     3601 ggccggacca tcgtcttcct gcagggcaca ctcccgcagg gaacgccgat cgtgcgcgca
     3661 atgccgaata cgccggccgc cgtcgggcgc agcatcaccg ttgcctgcgc gaacgcgatg
     3721 gtgaacacac gtcagcgcaa gctcgcctcc gatctgctct cggccatcgg caaagtggag
     3781 tgggcgaacg acgaaacgct gatggacgcg gtcacggcac tgtccgggtc ggggcccgcc
     3841 tatgtatttc ttctggctga agcaatggcc agcgccggga ttgcagcggg gctgcctgcc
     3901 gcgcttgcaa cgcgcctcgc gcacgacacg gtcgcgggct ccggggaact gctgcatcga
     3961 tcccagctca gcgccgcaac gttgcgggaa aatgtgacat cgcccggcgg gacgaccgct
     4021 gcggccctcg atgtccttct gggaccgggc gggcttgagg aactaatgac gaaggccata
     4081 gcggccgcga cacgccggtc gcgtgatctc gccggctagt cctgccctag aaccgtcccc
     4141 attccgcgcc tacattgatc gcagacgagt attgccatgg gaggtcgtca tggcccgcaa
     4201 gtccaagcgt ccgcgcagaa gcccggctgc gccgccctcg cgcggcggct ccgaccgcga
     4261 caaggcaatc gatgcgctta tgtcgctgct cggcgatcgc ccgttcgaag agatagggct
     4321 ggccgaaatt gccggcagag ccggcatcaa gctctcacag ttgcgtgccg aattcggatc
     4381 gacgctcgcg atcctggcgg cccatatcaa ggagatcgac cgcgcggttc tcgccggcgg
     4441 cgacggtgac atggccgaag agcccgcgcg cgagcgcctg ttcgatgtcc tcatgcggcg
     4501 cctggaggcg cttgcaccct acaaggacgc tatccgctcg cttatgcgct ctacgcggcg
     4561 acatcccggg ctcgcctttg cactcaatgc catggcagtc cgctcgcaac gctggatgct
     4621 ggaggccgcc ggcattggga cttccggccc gcgcggcgcg ctgcgggcac aaggcgcggc
     4681 gctcatgttt gcgcgcgtgt tgtccgtatg gatcgacgac gaggacgagg gtcttgaccg
     4741 gacgatggca acgctcgatc gcgggcttgc gagcgccgag cgttgggcag ggttcctcga
     4801 tgacctctgt gcgcttcctg cttgcgtctt gcgcggtccc cggcgccggc ggcgcatgcg
     4861 tgacgaagaa gcagaagccg cttagaccgg cacgcgaacg gtcgcgcgca atccgcccat
     4921 tggcgtgtcg cccaaggtga tatcgccgcc gtgcgaacgc gcgatgtcgc gcgcaatcgc
     4981 cagtcccagg ccggtgccgc cttcgtcctg attgcgtgca tcgtcgaggc gcaagaaggg
     5041 tttgaacact tcttcacgca tctcagccgg gatgcccggt ccatcgtcat caatcgtgac
     5101 cgtgagatag cggtgatcgc ggtggccgga aatggcgatt gacgccgcgt gacgtgccgc
     5161 attggaaacg agattggcaa ggcagcgctt gaaggccgcc gggcgcactg tgacgatcgg
     5221 cgggccgtgg aaaatcaccg tggccttgtg accatggcgc tctgcgtcga gacgcagctc
     5281 ctccaggaat tccgccatgt cggttggcgc cgccgtctcg ccgctgtcac cgcgcgcgaa
     5341 ggccagatag gcttccagca tccgcgccat ctcgtcgatg tctttttgca tcgcctcaac
     5401 ttcgggcgtc ttctttttga tgagagcgag ttcgagcttg aatcgcgtga gcacggtgcg
     5461 caggtcatgc gaaacgcccg ccagcatcgc cgtgcgctgc tcgatcgaac gctcgatgcg
     5521 agtcttcatc tcgatgaagg cggctgccgc gcggcggact tcgcgggcgc cgcggggacg
     5581 gaagttcggc acctccctcc ccttgccgaa gctttcggcc gcatcggcaa gtcgcaagat
     5641 cggtttgatc tgattgcgca ggaagatgat ggcgaccgtc agcagcaccg tcgacgtccc
     5701 caccatccag agcagaaaga tttccgaatt ggagacgtag gccgcactgc gccgggcaaa
     5761 gacccgcatc acggtgttgt cgagctgcac gcggatttcc agcagggcgg acttgccgac
     5821 ggtgtccatc cagtacggcc gcccgatttg cttgcgcaac tcctccgcca gagcctgatc
     5881 cagcaatgaa aagaagggtt tgggcccggg tggcggcatc tcggaaagcg gcagaaagtc
     5941 gaccaccagg ccaaggcgct gctgcgcgat ccgccggatc tgcgcctgat cggcatcctg
     6001 cggatagccg cgatagatat cgatgagcgc ggcgatatcc tggaccacgc tcgccgacag
     6061 gcgctgcgtg accacgttcc agtggcgctc catgaagacg aacgcgacga cagactgcag
     6121 gatcaccatc ggcgcgataa tgatgagcaa cgcgcgggcg tagaggccct tcggcatgac
     6181 gctg
//
LOCUS       DASSRL010000277         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_339926, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000277 DASSRL010000000
VERSION     DASSRL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339926"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFL49_05715"
     CDS             complement(<1..324)
                     /locus_tag="VFL49_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017273176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HET7911505.1"
                     /translation="MLGTVQTLAIILVALAMSLSLAHALEFPGKRRLGRDAYLAVQAI
                     YYPGFTIGGISEPLGVLAVLVLLFVTPRGTPAFWLTLAAVVALILMHATYWVMTHPVN
                     KFWLAG"
     gene            complement(411..791)
                     /locus_tag="VFL49_05720"
     CDS             complement(411..791)
                     /locus_tag="VFL49_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911506.1"
                     /translation="MRMTILPIALAALAGPAVAEPREVFGYGGELGEWEVSATVTEKE
                     SSPSRELSGPLTMTHIGICTQDGPEQKMGEIRFLVLDAPPRLRASLLVDGVSCTYSGK
                     LSNFYSGTLNCPGRAGVPLKLWLK"
     gene            complement(819..2399)
                     /locus_tag="VFL49_05725"
     CDS             complement(819..2399)
                     /locus_tag="VFL49_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7911507.1"
                     /translation="MKMSVAFAASAIAVVSLVRPAVGQSDDDNLGTVHFETSCTPEAQ
                     KLFDRGMLYQHSFWYRASQHEFEATLNADPSCGIAYWGIALSKLLNPHVPTPAKNLAE
                     GAAAITKGKAVGARTQRERDYIDALAVIYVDYDKLGHLPRTQAYAKAMEKVAQSYPND
                     DEAQIHYALALNTSASPADKTYANQLKGAAILEPIFARQPHHPGVAHYLIHLYDYPPI
                     AEKGLNAARAYAKIAPGAAHAQHMPSHIFTRVGYWKESIESNKVAQRVAKEASDFHDQ
                     LHAMDYQVYAYLQLGQDADARAVLDQMTAVTGFTETFLVGPYALAVSPARYALERGDW
                     KTAAGLKVRPSPLPQVQAVTYFARALGAARSGHPDAAQPEIAKLVELRETLRGKDAYW
                     TEQLDIQQKVASAWEIYAEGKYDDALKAMSAAADAEDKTEKHPVTPGAPIPARELYGT
                     MLLERGMASDALAAFEAVLKKEPNRLGAYAGAARAAAKAGDTRKARQYAEKVLAIAAN
                     ADKTRTDVSDAREFLKSH"
     gene            2545..4122
                     /locus_tag="VFL49_05730"
     CDS             2545..4122
                     /locus_tag="VFL49_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7911508.1"
                     /translation="MAIVMQDQAISFGQYRIVPRGGLMAGKREVRLTPKALALLSYLA
                     EHPNEVVTKDELFAAVWPKTDVGDAALVTCIQELRKALRDNARHPRYIETLHRRGYRF
                     IEKVSIGQPAGDGTQAAALQLPDCPSIAVLPFANMSEDPDQEHFADGISEDLITGLSR
                     IRWLFVIARNSTFVYKHRAIEVKQVARELGVRYVLEGSVRRAGRQLRVSAQLIDAISG
                     AHHWAEHYDREVDDVFAVQDQITNSVAAAIEPHLLAAEGVRALLRRSNDLGAWELVAR
                     AQTHFWRMTKDDYERAIDELKRAVDVYPDYAPARSLLAFCLMFAAHMGWTNRGRDLVL
                     GREHAMRAITLDDRDPWGHIALGYWALMEKRTEESIAAFRRAVEINPNSAAAHTHLGH
                     GLAFAGHDREAIEHAEGAIRLSPFDPMLALNLGTIAVSHFTAGRYAEAVKYTTEASRI
                     RPGFQGAHRLRCASLAQTGRIDDARAHFLIVRRQQPHLSADWIRANVPYQSPELMEQF
                     LEGMRKAGLDEEKIAVE"
     gene            complement(4141..>4242)
                     /locus_tag="VFL49_05735"
     CDS             complement(4141..>4242)
                     /locus_tag="VFL49_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET7911509.1"
                     /translation="AFVEKLTAAPNVRNVKTSLTLRSSKDAAMVPME"
BASE COUNT          812 a         1284 c         1313 g          833 t
ORIGIN      
        1 acccgcgagc cagaatttgt tcaccggatg cgtcatcacc cagtaggtcg cgtgcatcag
       61 gatgagcgca acgaccgcgg cgagggtcag ccagaacgcg ggcgtccctc tcggagtaac
      121 aaacagcagg acaagcaccg ccagcactcc gagaggctcg ctgataccgc caatggtaaa
      181 gccgggatag tagattgcct gcacggcaag ataggcatcc ctgccgagac ggcgcttgcc
      241 aggaaattca agcgcgtgcg caagcgagag cgacatcgcg agcgcgacaa ggataatcgc
      301 cagagtttgc accgtgccaa gcatggggtg cgccctggcg tagcaacgct gttctcgaac
      361 ggaagtttcc gtcgctcagc cgttgatttc tatccgaacc acacagcgta tcatttcagc
      421 catagcttca atggcacgcc tgcccggccc gggcagttca gtgttccgct gtagaaattc
      481 gacaacttgc cgctgtaggt gcagctgact ccgtcgacaa gcagtgatgc gcggagtcgg
      541 ggcggcgcat ccaaaaccag aaaccggatt tcgcccatct tctgctccgg accgtcctgc
      601 gtgcagatgc cgatatgcgt catcgtcaga ggcccggaca attcacggct gggagacgat
      661 tccttctcgg tgaccgtggc actgacctcc cattcaccga gctccccgcc atagccgaac
      721 acctcgcgcg gctcggcaac agccggacca gcaagtgccg caagcgcgat tgggaggatg
      781 gtcattctca tgatgccact cgctcggctg tgcggtcgtc aatgcgactt caggaattca
      841 cgagcatcgc taacatcggt gcgagttttg tcggcattcg ctgcaatggc cagcaccttc
      901 tccgcgtatt gccgcgcctt cctcgtgtct cctgccttgg cggcagcccg ggctgcgcca
      961 gcataggcgc cgagccggtt gggctccttc ttcaacaccg cctcaaaggc agcaagggcg
     1021 tccgacgcca tgccgcgctc gagcagcatc gtgccgtaca gttcgcgtgc agggatcggc
     1081 gcgcccggcg tcaccggatg cttttcggtc ttgtcttcgg cgtcggctgc cgcgctcatg
     1141 gccttgagcg catcgtcgta ttttccctcg gcatagattt cccaagccga tgccaccttt
     1201 tgctgaatgt cgagctgctc ggtccaatac gcatctttgc cgcgtagcgt ctcgcgcagc
     1261 tcaacaagtt tggcaatttc aggctgcgca gcgtcgggat gacctgagcg cgcggcgcca
     1321 agcgcacggg cgaaatacgt taccgcttgc acctgcggca acgggcttgg gcgaactttc
     1381 agtccggccg ccgtcttcca gtctccgcgc tcgagcgcat agcgcgccgg cgaaaccgcc
     1441 aatgcatatg gtccaaccag aaatgtctcc gtgaagccgg tgaccgccgt catctggtcg
     1501 aggactgccc tggcatctgc atcctgaccg agctgcaggt aggcatagac ctggtaatcc
     1561 atggcatgca gctgatcgtg gaagtcggat gcttccttcg cgacacgctg cgcgacttta
     1621 ttggattcga tcgactcctt ccaataaccg acgcgggtaa agatgtgcga cggcatgtgc
     1681 tgtgcgtgtg ccgcgcccgg tgcaattttt gcataggccc tcgcagcatt gagccccttc
     1741 tcggcgatcg gcggatagtc gtagagatga atgaggtaat gggcgacgcc gggatgatgc
     1801 ggttggcgcg cgaagatcgg ctccaggatc gccgcgcctt tcagctggtt ggcgtaggtc
     1861 ttgtctgccg gcgaagccga cgtgttgagc gccagcgcgt agtgaatctg cgcctcgtcg
     1921 tcgttcggat agctctgcgc gactttctcc atcgccttgg cgtaggcctg cgtgcgcggc
     1981 agatggccga gcttatcgta gtcaacgtag atgacggcaa gggcgtcgat atagtcgcgc
     2041 tcacgctgcg tcctagcacc gacagctttg cccttggtga ttgccgcagc gccctcggca
     2101 agatttttcg ccggcgtagg gacgtgcggg ttcaggagct tgctcagcgc gatgccccaa
     2161 taggcgatgc cgcatgaagg atcggcattc agcgtcgctt cgaattcgtg ttgcgaagcc
     2221 cggtaccaga acgaatgctg gtaaagcatg ccgcggtcga aaagcttttg agcttccggc
     2281 gtgcatgatg tttcgaaatg caccgtcccc agattgtcgt cgtcagattg cccgaccgcg
     2341 ggccggacca gcgacacgac ggcgatggcc gaagctgcga atgcgaccga cattttcatg
     2401 atggctacct ttccggaggt tccaaaaggc gggcgtaggt ccagcaccgc ccggcttttg
     2461 attgattttc gaccctagga gcgccgcctc cggaagtcat aaggaagaag caagatttct
     2521 ataggtgccc gtgatatctt tgaaatggcg attgttatgc aggatcaggc gatttcgttt
     2581 ggccaatacc ggatcgtgcc gcgcgggggc ctgatggccg gcaagcgcga ggtacggctc
     2641 acacccaagg cgctggcact cctgtcgtac ctcgccgaac accccaacga agttgtcacc
     2701 aaggatgagt tgttcgccgc ggtctggccg aagaccgatg tgggcgacgc agcgctggtc
     2761 acatgcatcc aggaattgcg caaggcgctg cgggacaacg cgaggcatcc ccgctacatc
     2821 gaaaccctgc accggcgagg ctaccgattc attgaaaaag tgtcgattgg acagccggcc
     2881 ggtgatggaa cgcaagcggc agccctgcag ctgcctgact gtccttccat cgctgtcctg
     2941 ccgtttgcca acatgagcga ggacccggac caggagcact ttgccgacgg catttcggaa
     3001 gacctgatca ctggcctatc ccgcatccgc tggcttttcg tgatcgcgcg caactccaca
     3061 ttcgtctaca agcaccgggc gatcgaggtg aagcaagtcg cgcgcgagct cggcgtgcgt
     3121 tacgtgctcg aaggcagcgt acgtcgggcc ggcaggcagt tgcgggtgag cgcgcaactc
     3181 atagatgcga taagcggcgc gcaccactgg gcggagcact acgaccgcga ggtcgacgat
     3241 gtctttgcgg tgcaagacca gatcaccaac agcgtcgccg cggccatcga accgcatttg
     3301 ctggccgccg aaggcgtgcg tgcgctgttg cgccggtcga acgatcttgg cgcctgggag
     3361 cttgtggcgc gcgcgcagac gcacttctgg aggatgacaa aagacgacta tgagcgggcc
     3421 atcgatgagc tcaagcgtgc ggttgatgtt tatcccgact acgcgcccgc ccgcagcctg
     3481 ttggcctttt gcctgatgtt tgccgctcac atggggtgga ctaaccgcgg ccgcgacctg
     3541 gttctgggcc gcgagcatgc gatgcgagcg atcacgctcg atgatcggga cccttgggga
     3601 cacatcgcgc tcggttattg ggcactgatg gaaaagcgca cggaagaaag catcgccgcg
     3661 ttccgacggg cggtcgagat caatccgaac tcggctgccg cccataccca cctcgggcac
     3721 gggctggctt ttgctggtca cgacagggag gcgatcgagc acgccgaagg agctatcagg
     3781 ctcagtccgt tcgacccgat gcttgcattg aacctcggca ccattgcggt gagccacttt
     3841 acagccggac gttatgccga agcggtcaag tacacgacgg aagcgtcgcg gatccggccg
     3901 ggatttcagg gcgcgcatcg gctgcggtgc gcgagccttg cgcaaaccgg tcgcatcgat
     3961 gacgcgcgcg cgcatttcct gattgtgcgg cgccagcagc cacatctgtc ggctgattgg
     4021 atcagagcaa acgtgcccta ccaatctcct gagctgatgg agcagtttct tgagggtatg
     4081 cgcaaggcag gtctggacga ggaaaagata gccgtggaat gacgcggcta ccgctcgcca
     4141 tcattccatc ggcaccatcg ccgcgtcctt ggaactgcgc agcgtgagcg aggtcttcac
     4201 gttgcgcacg ttcggcgcag cggtgagctt ctcgacaaag gc
//
LOCUS       DASSRL010000278         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1721941, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000278 DASSRL010000000
VERSION     DASSRL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721941"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1112
                     /locus_tag="VFL49_05740"
     CDS             60..1112
                     /locus_tag="VFL49_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HET7911510.1"
                     /translation="MRMFQVCVCGQPLQLNEQPTPEPKGTEVLLKVLAAGVCHSDLHL
                     ADGWFDLGGGKRMSLADRGMKLPVTLGHENVGEVVSVGPDVKGVKAGDRRLAHPWIGC
                     GTCDACKRGEENLCRAMRSLGVFSNGGYADYLMVPHPRYLFDIGDLPPERAAPLACSG
                     VTTYGALKKIPTLKSEPTVIIGAGGLGLMCLALHKAMGGHSAIVVDIDPAKRDAARKA
                     GAAKVVDGGAPDASQQIIDMTNGGAWGVIDLVGSSQSARVGYDSLSKGGKYVVVGLYG
                     GDLTVSLPPIPMRALTIQGSYVGSLPEMKELIELVQRTGIPPVPVATRPLEDVNAVMD
                     QLRAGKVIGRVVLTPN"
     gene            1123..1632
                     /locus_tag="VFL49_05745"
     CDS             1123..1632
                     /locus_tag="VFL49_05745"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7911511.1"
                     /translation="MDASGLRALQAPIKERYKNDPKAARITLMARGNLDETNIACKVD
                     TGRALAIAGLHPATGGSGLELCSGDMLLEALVACAGVTMKAVATALDIPLKSASVSAE
                     GDLDFRGTLGVAKDAPVGFAQIRLRFAVDTDAPQEKLDQLLKLTERYCVVYQTIKNGP
                     PVDVKLARL"
     gene            1704..2498
                     /locus_tag="VFL49_05750"
     CDS             1704..2498
                     /locus_tag="VFL49_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911512.1"
                     /translation="MPPDLYFALTLIAKMAITAAFVLAATITAERAGPLVGGLVATLP
                     IGAGPVYVFLALDHDAHFISQSAVASLAVNAANVIFAVVYCVLAQRRSLAVSLGGTYI
                     VWIVLTAIVTASHWALGPALLINAAAIAVGIWIVRPFRHTPMPRQQPRWYDYALRALM
                     VAVLVGIVVTFSFRIGSYGSGLLAVFPVVLTSIILIFHRRAGGKPTAAVLANAPLGLL
                     GFGIACMVLHLTAEPLGVVTGLSLALATSIGWSFAVLAARRQGIAV"
     gene            2502..>3268
                     /locus_tag="VFL49_05755"
     CDS             2502..>3268
                     /locus_tag="VFL49_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911513.1"
                     /translation="MSLFLFGLLLKIATTASIVVAASVIVERSGPFVGSLIAALPTAG
                     GAAMIILALEHPPEFIAQSAVGSLIANAACAFFALTYAALAQRQSLQLSLGGAFLVWL
                     VIVFLLRLVEWNAIGAVLLNAVVYPIAIVASRRFRIAGAVKRVELTGIDLAWRAGVVT
                     LCVVLVTAASSSIGSYFSGTFAFFPVAMGSFFIILHPRIGGPAAASVAAHVLTPLIGL
                     GLGLLAVHLLATTLGVWWSYALGLSIGVGWNALLWVLR"
BASE COUNT          514 a         1007 c         1081 g          666 t
ORIGIN      
        1 ggccgcggag tgtggttcgg tgtcgggacg atccagcgat gccgggagaa taaaagacga
       61 tgcgcatgtt ccaggtctgc gtttgcggtc agccgttaca gctcaatgag cagcccacgc
      121 ccgagccgaa aggcacggaa gtcctgctga aggtcctggc tgccggtgtc tgtcatagcg
      181 atctgcacct tgccgacggc tggttcgatc tgggcggcgg caagcggatg agcctggccg
      241 atcgcggcat gaagcttccg gtcacgctcg gtcacgaaaa cgtcggggag gtggtctccg
      301 tcggtccgga cgtcaaaggc gtcaaggccg gtgatcgccg gctcgcgcat ccttggatcg
      361 gctgcggcac ctgcgatgcc tgtaaacgcg gcgaggaaaa cctgtgccgc gccatgcgca
      421 gtctcggcgt cttttcaaac ggcggttacg ccgactatct gatggtcccg catccgcgct
      481 atctgttcga catcggcgac ctgccgcccg agcgcgcggc gccgcttgcg tgttccggcg
      541 tcacgacata tggagctttg aaaaagattc cgacactgaa aagcgagccg accgtcatca
      601 tcggtgcggg aggccttgga ctcatgtgtc tcgcgctgca caaggcgatg ggagggcaca
      661 gcgccatcgt cgtcgatatc gatccggcga agcgcgacgc cgcaaggaaa gcgggtgctg
      721 caaaggtcgt cgacggcggc gcgcccgatg cctcgcagca gatcatcgat atgaccaatg
      781 gtggcgcctg gggtgtgatc gatcttgtcg gctcctcgca gagcgcgcgc gtcggctatg
      841 acagcctgag caagggcggc aaatatgtcg tcgtcgggct ctatggtggc gacctgacgg
      901 tgtcattgcc gccgatcccc atgcgggcgc tgacgatcca gggttcctac gtcggcagcc
      961 tgccggagat gaaagagctg atcgaactcg tgcagcgcac ggggattcct ccggttcccg
     1021 tcgcgacccg tccgctcgaa gatgtgaacg cggtcatgga tcagcttcgc gccggcaagg
     1081 tgatcggccg cgtggtgctg acgccgaatt gaggagcgca tcatggatgc gagcgggctg
     1141 cgcgcgctgc aggcgccgat caaggagcgt tacaaaaacg acccgaaggc cgcacgcata
     1201 acgctcatgg cgaggggcaa tctcgacgag acgaacattg cttgcaaggt cgacacggga
     1261 cgcgcgctcg cgatagcggg cctgcatccg gcgaccggcg gctcaggcct ggaactttgc
     1321 tccggcgaca tgctgctgga ggcgctggtc gcctgtgccg gcgtcaccat gaaagcggtg
     1381 gcgaccgcgc tcgatattcc gctcaaatcg gcaagcgtct cggcggaagg tgatctcgat
     1441 ttccgcggca cgctcggcgt cgccaaggat gcaccggtcg gctttgcgca aatccgcctg
     1501 cgcttcgctg tcgacactga cgcgccgcag gaaaagctcg atcagctact caagctcacc
     1561 gagcgctatt gcgtggtcta ccagaccatc aagaacgggc cgccggtcga cgtaaagctt
     1621 gcacgcctct agcgccgacg gtctgctatg catggttgca acttgcacca cgcctgatgg
     1681 cgtggtcatg tgcgccgccc cagatgcctc ccgacttgta tttcgcactc acgctcatcg
     1741 ctaaaatggc catcaccgcc gcgttcgtac tggcggcgac aatcacggcc gagcgcgccg
     1801 ggccgctggt cggcgggctg gtggcgacgc tgccgatcgg cgcgggaccg gtctacgtgt
     1861 ttcttgcgct cgaccacgac gcgcatttca tatcgcaaag tgcggtcgcg agccttgccg
     1921 tcaatgcggc gaacgtcatt ttcgcggtgg tttattgcgt gctggctcag aggcgatcgc
     1981 tcgccgtcag tttgggaggc acctacattg tatggatcgt gttgacggcg atcgttacgg
     2041 ccagccactg ggcgctcggt cccgcgcttc tgatcaacgc ggccgcaatc gccgtgggaa
     2101 tctggatcgt gcggccgttc cggcacacgc cgatgccgcg gcagcagccg cgctggtacg
     2161 actacgcttt acgcgcactg atggtcgccg tcctggtcgg catcgtcgtc acattcagct
     2221 tccgcatcgg gtcatatggc agcggcttgc tggcggtgtt tccggttgtg ctcaccagca
     2281 tcatcctcat tttccatcgc cgggccgggg gcaagccgac tgcggcggtg ctcgccaatg
     2341 ccccgctcgg cctacttggt tttggaatag cctgcatggt gctgcatctg accgccgagc
     2401 ccctgggtgt cgtgacaggg ctttcgctgg cgctggcgac atcgatcggc tggagctttg
     2461 cggtgctggc cgcgcgtcgc cagggaatcg cggtttagca tatgtctctt tttctgttcg
     2521 gattgttgct gaagatcgcg acgactgcgt cgatcgtggt ggcagcttcg gtcatcgtcg
     2581 agcgcagcgg tcctttcgtc ggctcgctca tcgcggcgct tccgacagcc ggcggcgcgg
     2641 cgatgatcat cctagcgctc gagcatccgc ccgaattcat cgcgcagagc gcggtgggaa
     2701 gcctgatcgc gaacgccgct tgcgcgttct ttgcgctgac ctacgctgcg ttggcgcaac
     2761 ggcaatcgct gcaactcagc ctcggcggtg cgttcctggt gtggctcgtc atcgtgtttc
     2821 tgctgcggct ggtggagtgg aacgcgattg gtgcggtact gctcaacgcc gtcgtctatc
     2881 cgattgcgat tgtcgcttcg cgacgctttc gaattgcggg tgcagtgaaa cgcgtcgagc
     2941 tgaccggcat cgaccttgcc tggcgcgccg gcgtggtgac gctctgtgtc gtgctcgtca
     3001 ccgcggcgag cagctcgatc gggtcatatt tttccggcac gtttgcattt ttcccggtcg
     3061 cgatgggctc attcttcatc atcctgcatc cgcgcatcgg cgggcccgca gcggcgagcg
     3121 ttgcggcaca cgtgctcacg ccgctgatcg gcttgggtct cggcctgctc gccgtgcacc
     3181 tgctggccac gacactcggc gtgtggtggt cctatgcgct cggactgagc atcggcgttg
     3241 gctggaacgc gctgctgtgg gtgctgcg
//
LOCUS       DASSRL010000279         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1319804, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000279 DASSRL010000000
VERSION     DASSRL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319804"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="nrfD"
                     /locus_tag="VFL49_05760"
     CDS             complement(<1..924)
                     /gene="nrfD"
                     /locus_tag="VFL49_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7911514.1"
                     /translation="MTAKAATHPILPPAQTYADVTDQLASIPLHFPSRRRWLVALFVS
                     SLLLGLLFVAATVLFATGVGIWGLNIPVNWAFAIHNYVWWLGIGHAGTLISALLLLLG
                     RDWRNSLNRFAETMTLFAVVCAGMYPILHLGRPWFVYWMFPYPATMKVWPQFRSPLEW
                     DIWAVLTYLTVSIVFWYIGLVPDLAAARDRARRRRWKVFFGILSLGWRGSALHWMRWH
                     QAYRLLAALAVPLVVSVHSEVSLLFAVGTIPGWHSTIFPPYFVLGAAFSGFAVVSMIA
                     IALRALFHLDNLVTDDHLDILGKVLLATGLMT"
     gene            complement(921..>3456)
                     /locus_tag="VFL49_05765"
     CDS             complement(921..>3456)
                     /locus_tag="VFL49_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7911515.1"
                     /translation="GASDAFMQSALFELYDPERSKIPLMNGAPSSWNAFAGVLNELRA
                     RWRERQGEGLRILMGSASSPTLIRQLGELAKQFPKMRWSQFSAVASAQEAKAMTLNFG
                     RPAASHLVLDQCDVIVSLGHDLLGPGPHQVVRASAWAKRRGEVPPGSGRSRLHVAESL
                     PSLTGTVASTRLAGEPDRIAALARAVAAEFKIEGFAQPHLEAGERNWLDRAVRELRDH
                     GGHSLLTIGPQFDAHLQAIAPLVNEQLKNVNATVWYSDPILPAGRGAQSLSDLTAEIE
                     SGVVDTLIVLDCNPVYAAPGMLKFPSQMVRVPNRIHAGMHPDETAQLCQWHLPLSHAL
                     ECWSDGRAVDGSASIVQPVVAPLYASRSVHQLADLLLGVSDPSADAAVRATWQARFGP
                     AFDEQWTRALHDGFVADTGARPLTVSARPPGQIAENPNSGPLHIVFKPDPCVWDGRFA
                     NIAWLQELPKPLTKITWDNAIEISPAIAAEYKLSNGDIAEVTVAGRRVRGAVWIMPGQ
                     AARTIALSFGYGRRAGGDIATGSGYDAYSVRPDENSWLTSGTIMRVGGEYRIATTQAH
                     HRMDGFDFVREVTAGNPRTPAPKDQSTFYPDWNGEQANGAVAEHAWGMVIDLDLCIGC
                     NACVSACNVENNVLVVGKDQVARGREMLWLRVDRYYTGAPENPRTYFQPVPCMHCEKA
                     PCEMGCPVHATAHSPEGVNQMVYNRCIGTRTCSSYCPYKVRRFNWYDYRHFAEAEQAV
                     KNPDVTVRSRGVMEKCTYCTQRIQAAHVQADKDNRALRRDEIVTACQQACPTKAITFG
                     DLKDEGSAAAKRRRSGRHYVLLEELGTRPRTTYLARWNDSPDGGGA"
BASE COUNT          662 a         1100 c         1116 g          578 t
ORIGIN      
        1 ggtcatcagg ccggtagcca gcagaacctt gccgagaatg tcgagatgat cgtcggtgac
       61 gaggttgtct agatggaaca gcgcgcgcaa ggcgatcgcg atcatcgaca cgacggcaaa
      121 gccggagaac gctgcgccga gcacgaagta gggcgggaag atcgtcgaat gccagccggg
      181 gatcgtgccg accgcaaaca gcaatgagac ttcgctgtgc accgagacga ccagcgggac
      241 ggcgagcgcc gccagcaggc gataggcctg atgccaccgc atccagtgca atgccgagcc
      301 gcgccagccg agcgacagga tgccgaaaaa caccttccag cgccggcgcc gggcgcgatc
      361 gcgcgccgcg gcaagatcgg gaacgagacc gatgtaccag aacacgatcg agacggtgag
      421 ataggtcagc accgcccaga tgtcccactc gagcgggctg cggaattgcg gccacacttt
      481 catggtcgcc ggatacggaa acatccagta cacgaaccat ggccggccaa gatgcaggat
      541 cggatacatg ccggcgcaga ccaccgcgaa cagcgtcatc gtttcggcga agcggttaag
      601 cgaattgcgc cagtcgcggc cgagcagcag cagcagcgct gagatgagtg tgccggcatg
      661 gccgatgccg agccaccaga cgtaattgtg gatcgcgaag gcccagttga ccgggatgtt
      721 gagaccccag atgccgacgc cggttgcgaa caaaacggtc gcggcgacga acagaagtcc
      781 aagcagcagc gacgacacaa acagcgcgac cagccagcgt cgccgcgacg gaaaatgcag
      841 agggatgctg gccagctgat cggtgacgtc ggcataagtt tgcgcgggcg gcaagatggg
      901 atgtgtcgct gctttcgccg tcatgccccc ccgccatccg gggaatcatt ccagcgcgcc
      961 agataggtcg tgcgcggacg cgtccccaat tcctcgagca gcacatagtg ccggccgctg
     1021 cgccggcgct ttgccgcagc cgagccttcg tctttcaggt cgccgaaggt gatggccttg
     1081 gtggggcagg cctgctggca ggcagtgacg atctcgtcac ggcgaagcgc gcgattgtct
     1141 ttgtcggcct gcacgtgggc agcctggatg cgttgcgtgc aataggtgca tttttccatg
     1201 acgccgcggg agcgcaccgt gacatcggga ttcttcaccg cctgctcggc ttccgcgaag
     1261 tggcggtagt cgtaccagtt gaagcggcgc actttataag ggcaatagct cgagcatgtg
     1321 cgcgtcccga tgcagcggtt atagaccatc tggttgacgc cttccggcga gtgcgcggtg
     1381 gcgtgcaccg ggcagcccat ttcgcagggc gccttctcgc aatgcatgca cggcaccggc
     1441 tgaaaatagg tgcgcgggtt ttccggtgcg ccggtgtagt aacggtccac gcgcagccac
     1501 agcatctcgc gcccgcgcgc gacctgatcc ttgccgacaa ccagcacgtt gttctcgacg
     1561 ttgcaggccg aaacgcaggc gttgcagccg atgcagaggt cgaggtcgat caccattccc
     1621 caggcgtgct cggcaacggc gccgtttgcc tgctcgccgt tccagtcggg ataaaacgtc
     1681 gactgatcct tcggcgcagg cgtgcgcgga tttcccgcag tcacctcgcg cacaaagtcg
     1741 aagccgtcca tgcgatgatg tgcctgcgtc gtggcaatgc ggtattcgcc gccgacacgc
     1801 atgatcgtcc cgctcgtcag ccaggaattt tcgtcggggc ggaccgaata ggcgtcgtag
     1861 ccgctgccgg tcgcgatatc gccgccggcc cgccgaccat aaccaaatga cagcgcgatc
     1921 gtgcgcgcag cctgaccggg catgatccag accgcgccgc gcacgcgccg gcccgccacc
     1981 gtcacctcgg cgatatcgcc gttcgaaagt ttgtattcgg cggcaatcgc cgggctgatc
     2041 tcgatcgcgt tgtcccaggt gatcttggtg agaggcttcg gcaattcctg cagccaggca
     2101 atgttcgcaa aacgtccgtc ccagacgcag ggatccggct taaacacaat gtgcagaggg
     2161 ccggagttcg gattttccgc gatttggccc ggaggcctgg cggacaccgt caaaggccgg
     2221 gcaccggtat cggcgacgaa gccgtcatgc aaggcacggg tccactgctc atcgaaggcc
     2281 gggccgaacc ttgcctgcca ggtggcgcgc accgcggcgt cggctgacgg atcgctgacc
     2341 cccagcaaaa ggtccgcgag ttgatgaaca ctgcgcgagg cataaagcgg cgccacgacc
     2401 ggctggacga tgctcgcact gccgtcgacc gcgcgcccgt cggaccagca ctccagcgca
     2461 tgcgaaagcg gcaaatgcca ttggcataat tgggcggttt cgtccggatg catccccgca
     2521 tgaatgcggt tgggcacgcg aaccatttgg ctgggaaatt tcagcatgcc gggcgcggca
     2581 tagacgggat tgcagtcgag cacgatcagc gtgtcgacaa cgccgctctc gatttccgcc
     2641 gtgaggtcac ttaaagattg cgcgccgcgg ccggccggga gaattgggtc gctgtaccat
     2701 accgtcgcat tgacgttctt cagctgctcg ttcaccagcg gcgcgatcgc ctgcaggtgc
     2761 gcatcaaatt gcgggccgat ggtcagtagc gaatgaccgc cgtggtcgcg cagctcgcgc
     2821 accgcgcggt cgagccagtt gcgctctccg gcctccaggt gaggctgcgc aaagccctcg
     2881 atcttgaact ccgccgcaac agcccgcgcc agcgcggcaa tgcgatcggg ctcgccggca
     2941 agccgcgtgg atgccacggt tccagtgaga cttggcaggc tttccgcgac gtgcagtcgg
     3001 ctgcgcccgc tgcctggcgg gacctcgccg cgccgcttcg cccaggcgct tgcgcgcacc
     3061 acctgatgtg gccctggtcc gagcaggtcg tggccgaggc tgacaatcac gtcgcattgg
     3121 tcaagcacga gatgcgacgc cgccggccgc ccgaaattga gcgtcatcgc tttggcttcc
     3181 tgtgccgagg cgaccgcgga aaattgtgac caacgcattt tcggaaactg cttcgccagc
     3241 tcgccaagtt ggcggatcaa ggttggcgat gacgccgacc ccatgagaat ccgcaggccc
     3301 tcgccctgcc gttcgcgcca gcgtgcgcgc aattcgttca acacgccggc gaaggcattc
     3361 cagctcgacg gcgcaccgtt catcaacgga atcttcgacc gttccggatc gtagagctcg
     3421 aaaagtgcgc tctgcatgaa tgcatcgctc gccccg
//
LOCUS       DASSRL010000280         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1232324, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000280 DASSRL010000000
VERSION     DASSRL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232324"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFL49_05770"
     CDS             <1..402
                     /locus_tag="VFL49_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7911516.1"
                     /translation="TVTFVAGLRLDGFTAPFAIDCAMNGDIFVEYVQQCLAPALKPGD
                     IVVIDNLPAHKRDEVRMIIEAAGATLRYLPPYSPDLNPIEQSFAKLKAHLRKAKERTI
                     PALYDQIGQSLETFQPTEFRNYFRKSGYVPA"
     gene            complement(415..1245)
                     /locus_tag="VFL49_05775"
     CDS             complement(415..1245)
                     /locus_tag="VFL49_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7911517.1"
                     /translation="MLLGYPISEIAWLALWIVAAGVLVGILAGLFGIGGGAIIVPVLY
                     EVFRVLGVPEDVRIQACIGTSLAIIVPTTVRSYLGHKKKGSVIPEVVRIWALPAIVGV
                     AIGSLTASFAPAFVFKIAFLVFVTFIAVRMLFGGERWKLGDQLPGRGILAIYGLITGL
                     FSSLVGVSGGAISNAVLTLYGRPMHQAVGTSAGVGVPITIAGTLGYVLAGWPHMAQLP
                     PLSIGYVSLVGLVLMAPVSSYTTSYGVRLAHWLPRRKLEIAFGVFLILVALRFAASMI
                     "
     gene            complement(1266..2231)
                     /gene="dusA"
                     /locus_tag="VFL49_05780"
     CDS             complement(1266..2231)
                     /gene="dusA"
                     /locus_tag="VFL49_05780"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HET7911518.1"
                     /translation="MMEWTDRHCRYFHRLLTRHALIYTEMLTTGAVLHGDRARLLGFD
                     EFEHPVGLQLGGCDPHALAQSARIGAEFGYDEINLNVGCPSDRVQEGRFGACLMAEPR
                     LVGDCVVAMKAAVAVPVTVKCRIGIDEQDPESTLFTFAETVKAAGADALVVHARKAWL
                     KGLSPRENREVPPLDYPLVHRLKAAHPNLEIVLNGGIVSLEQAHAHLGALDGVMMGRA
                     AYQEPWRLLGVDPLFYGATAPFKTPKGAAAALIPYIERQLDSGLRLHAVTRHVLGLFR
                     GVPGARAFRRHLATAGVKPEARAHVMAEALALVLDSGDDLAHIAA"
BASE COUNT          498 a          736 c          650 g          390 t
ORIGIN      
        1 acggtgacct ttgtggccgg gttgcggctg gacggcttca cggcgccgtt cgccatcgac
       61 tgcgccatga acggcgacat cttcgtcgaa tatgtgcagc agtgcctggc accggcgctt
      121 aagcccggcg atatcgtcgt catcgacaat ctgccggccc acaagcgcga tgaggtgcgc
      181 atgatcatcg aagcagccgg cgcgacattg cgctatctgc cgccctacag ccccgatctc
      241 aacccgattg agcagagctt cgccaagctc aaggcccatc tgcgcaaggc caaggagcgc
      301 accatccctg cgctctatga ccagatcgga cagtccctcg aaaccttcca gccaaccgaa
      361 ttccgcaact acttcagaaa atctggatat gttccagcct gataggattc tgctctagat
      421 catgctggcg gcaaagcgca gtgcaaccag gatcagaaaa actccaaatg cgatttccag
      481 ttttctccgt ggcagccagt gcgccaggcg cactccatag cttgtcgtgt agctggagac
      541 cggcgccatt agtactaatc caacaagcga cacgtagccg atcgacaaag gtggcaactg
      601 cgccatatgc ggccagcctg caagcacgta gccgagtgtc ccggcgatcg tgatcggcac
      661 gcctacaccc gccgacgttc cgactgcctg atgcatcggc cgtccgtaga gcgtgagcac
      721 cgcgtttgag atggcgccgc cgctcacgcc gacgagagat gagaagagcc ccgtgatcag
      781 accgtaaatc gccagtatgc cgcggcccgg gagctggtcg ccaagtttcc agcgctcgcc
      841 gccgaacagc atgcgtacag cgataaacgt gacaaatacg agaaaggcga tcttgaatac
      901 aaaggccggt gcaaatgagg ccgtcagcga gcctattgcg acaccaacta tcgcaggcag
      961 cgcccagatg cgcaccacct cgggaatgac cgaacctttc ttcttgtgac cgagatagga
     1021 gcgtaccgtc gtcggcacga tgattgcgag cgaagtgccg atacaggcct ggatgcggac
     1081 atcctcaggc actccgagca cgcggaatac ctcatatagc accggcacaa tgatggcgcc
     1141 gccgccaatg ccaaacaaac ctgcgagaat tccgaccagt actccggccg caacgatcca
     1201 aagcgcgagc caagcgatct cgctgatcgg atatcccagc agcatgtgtc tccggcaggc
     1261 ttatgttacg cggcgatgtg cgccaaatcg tcgccgctgt ccagcaccag tgcgagcgct
     1321 tcggccatga catgcgcgcg ggcctcgggc ttgaccccgg cggtcgccag atgccgtcgg
     1381 aacgcccgcg ctcccggcac gccacggaac aggccgagca cgtggcgcgt gaccgcgtgc
     1441 aaacgcaacc cactgtcgag ttgacgctcg atatacggaa tgagagcggc cgcggcaccc
     1501 ttaggcgtct tgaacggtgc ggtggctccg taaaacaacg ggtcgactcc gagaaggcgc
     1561 cagggttcct ggtaggctgc ccggcccatc atcaccccat cgagcgctcc aagatgcgca
     1621 tgcgcctgct ccaggctgac gattccccca ttgagcacaa tttccagatt cggatgtgcc
     1681 gccttcaacc gatgcaccag cggatagtcg agtggcggca cctcacgatt ttctcgtggc
     1741 gacaagcctt tcagccaggc cttgcgcgcg tgcacgacca aggcatcggc gcccgccgcc
     1801 ttcacggtct cggcaaaagt aaaaagcgtg ctttccggat cctgttcgtc aatgccgatc
     1861 ctgcacttga cggtcaccgg caccgcaacg gcggctttca tcgcgaccac acaatcgccg
     1921 accagccgag gctcggccat caggcaggct ccgaaccggc cttcttgaac gcgatcggac
     1981 gggcaaccaa cgttgagatt gatttcgtcg taaccgaact ccgctccgat gcgggctgat
     2041 tgcgcaagcg catgcggatc gcacccgccg agttgaaggc cgacgggatg ctcaaattcg
     2101 tcgaaaccaa gaagacgcgc acggtcaccg tgcagcaccg cgcccgtcgt gagcatctcg
     2161 gtgtagatca gcgcatgccg tgtgaggaga cggtggaaat accggcagtg ccggtccgtc
     2221 cactccatca taggagcgac acagaaacga tgggacagac tctgcggcgc tttc
//
LOCUS       DASSRL010000281         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_742502, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000281 DASSRL010000000
VERSION     DASSRL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742502"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFL49_05785"
     CDS             complement(<1..740)
                     /locus_tag="VFL49_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016553229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7911519.1"
                     /translation="MFAGLTFGPFTFDAQRGRLLRQGRPVAVSSKGLRLLEAFLAAPG
                     HVLKKSELMRAGWGDAAVEESNLSVQIAALRKQLGPAADGSDWITTVPRVGYRFVGFS
                     GQVPVEGAAAPKPSPVERDHRPSIAVLPFANSGGDKDQDYLADGITEDIITALIRFRW
                     FFVIARNSSFAYKDKALDAKQIAHELGVRYLLQGSVRKSGQRIRISSQLIEAASAKHI
                     WAERYDLELTEAFAVQDEIAERVAGAIEP"
     gene            861..1307
                     /locus_tag="VFL49_05790"
     CDS             861..1307
                     /locus_tag="VFL49_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016553230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7911520.1"
                     /translation="MTMLDAARTDDNTVSAPRKASVVKGAAGYRAEQGSDYEPGVSAE
                     TVGSKSIWLGMISLLPGKRTRAHVHEFHETALYMLSGDEMELWTGDQLQYRDVVMPGD
                     YIFIPANMLHVAVNRGRKPAVFVGSRNEATAQESMVLRPEMDGKVP"
     gene            1408..1710
                     /locus_tag="VFL49_05795"
     CDS             1408..1710
                     /locus_tag="VFL49_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911521.1"
                     /translation="MKALHVAAFSFAAALAALAIQAAQAQDNSNVRQDNYVRKVPLED
                     FKVPVVPIIPPGSQLDLRPGRTPDSADRIYDSTPFSRDPTTPSIGLSIKSPFDDRK"
     gene            complement(1682..>2417)
                     /locus_tag="VFL49_05800"
     CDS             complement(1682..>2417)
                     /locus_tag="VFL49_05800"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7911522.1"
                     /translation="SIRLACQLRPQSDIGVIPILPSSMNANLLRKGRRMNIGKERYIV
                     SMFVDMRGSTKLAEARLPFDVVFLINRFLAAVSQAALDAGGQPNQFIGDGTLALFGLE
                     TAPETACRQALRAAAKVAANVEQMNRQLKADLREPIQYGIGIHGGVVIVGDIGFKDHT
                     VFTALGDPVNVAARLQDLTKSLNCKVVISEDVCVTAGIAVDALARQQVVIRGRDEPMI
                     VRCVDDPAELVALVDGAAISGRQTAT"
BASE COUNT          480 a          774 c          725 g          438 t
ORIGIN      
        1 ggctcgatcg cacctgccac ccgctcggca atctcgtcct gcaccgcaaa ggcctccgtc
       61 agctcgagat catagcgctc ggcccagatg tgctttgcgg acgcggcctc tatcagctgc
      121 gaggatatgc ggatgcgctg acctgacttt cgcacgctgc cttgcagcag atagcgcacg
      181 cccagctcat gcgcgatctg cttggcgtcc agcgccttgt ccttgtaagc gaaactcgag
      241 ttgcgcgcga ttacgaaaaa ccagcgaaaa cggatgagcg cggtaataat gtcttcagtg
      301 atgccatcgg cgagatagtc ctgatccttg tcgccgccgg aattggcaaa gggcaggacg
      361 gcaatcgatg gtctgtgatc ccgctcgaca ggcgaaggtt tcggcgccgc ggccccctca
      421 accggcacct gtcccgagaa cccgacgaac cgatagccga cccggggcac cgtcgtgatc
      481 cagtcgctgc cgtccgccgc cggcccgagc tgcttcctga gcgccgcgat ctggaccgaa
      541 aggttgcttt cctcgaccgc cgcgtcgccc caacccgccc gcatcagctc cgattttttc
      601 agcacgtgac cgggcgcagc aagaaatgcc tccagcaggc gcagcccctt gctgctcacg
      661 gcgaccggcc ggccttggcg gagaagcctt ccccgctgcg catcgaaggt gaaagggccg
      721 aaggtcaaac cggcaaacat gcgggcagac taatgggttt cgcaaaaatt cggaactttt
      781 cggacctgga ttcaagactt ctggatagct gccagcgatc ttgcgcgcac tcgatccaga
      841 taaccgcaca ggagatttcg atgacaatgc ttgacgccgc acgaaccgac gacaatacag
      901 tgtccgcccc gcgaaaggcc tcagtagtca agggcgccgc tggctaccgc gccgagcaag
      961 gcagtgacta cgagccgggc gtgagtgcgg aaaccgtcgg ctcgaaatcg atttggctcg
     1021 ggatgatctc gttgctaccc ggcaagcgga cacgcgcgca tgtgcacgag tttcacgaaa
     1081 ccgcactcta catgctcagc ggcgacgaaa tggaattatg gacgggcgac cagcttcaat
     1141 atcgcgacgt ggtgatgccg ggcgattaca tctttatccc cgcaaatatg ctgcatgtcg
     1201 ccgtgaaccg aggccgtaag ccggcggtct tcgtcggctc gcgcaatgag gccaccgcac
     1261 aggaaagcat ggtgttacgg cccgagatgg acggaaaagt gccttagctc atcgtcgcgc
     1321 tgtccggcca aaaaggctat ttcggcggtg cgcttggtgg tagattttgc cggtggccgg
     1381 cccgaaaccg gccttcggga ggcggcgatg aaagctctcc acgttgcggc tttttccttc
     1441 gcggctgcgc tcgcggctct ggcaatccag gccgctcagg cgcaagacaa cagcaacgtt
     1501 cgccaggaca attacgtccg caaggtgccg ctcgaggact ttaaggtccc ggtcgttccg
     1561 attattccgc cgggctcgca actcgacctg cggccgggcc gcacgccgga ttcggccgac
     1621 cgtatctacg actccacgcc gttctccaga gacccgacga cgccttcgat cgggctgtca
     1681 atcaagtcgc cgtttgacga ccggaaatag ccgcaccgtc gaccagcgcc acaagctccg
     1741 ccggatcatc gacacaacgc acaatcattg gctcgtcacg gccgcggatg acaacctgct
     1801 ggcgtgcgag tgcgtcaacc gcaatgccgg cggtgacgca cacgtcttcc gaaatgacga
     1861 ccttacagtt gagcgatttc gtcaaatcct gcagccgcgc ggccacattc accggatcgc
     1921 cgagcgcggt gaacacggtg tgatccttga agccgatgtc gccgacgatc acgacgccgc
     1981 catgaatacc gatgccgtac tgaatcggct cgcgcaggtc ggccttcagt tgccggttca
     2041 tctgctcgac attggcggcg acctttgcgg ccgcgcgcag cgcctgacgg cacgccgtct
     2101 caggggctgt ctcgaggccg aacagcgcca gcgtcccgtc gccgatgaac tggttgggct
     2161 ggccgcccgc atcaagcgcg gcctgcgaaa cggcggcgag gaaacggttg atcaggaaca
     2221 cgacatcgaa cggcagccgt gcttcggcga gcttggtcga gccgcgcatg tcgacgaaca
     2281 tgctgacgat atagcgttcc ttgccgatgt tcatgcggcg gcccttgcgc agcaaatttg
     2341 cattcatgct tgacggcaga atcgggataa cgccgatgtc cgactgcggt cgcagctggc
     2401 atgcgagccg gatcgat
//
LOCUS       DASSRL010000282         7917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_427331, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000282 DASSRL010000000
VERSION     DASSRL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427331"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFL49_05805"
     CDS             <1..1320
                     /locus_tag="VFL49_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7911523.1"
                     /translation="GMNYFMEIAKLRAARMLWAKLMKPFKPEDVRSLSLRTHCQTSGW
                     SLAAQDVFNNVARTMIEAMAATQGQTQSLHTNALDEALALPTDFSARIARNTQIVLQQ
                     EAGATRIIDPWGGSYYVERLTYELARKAWGHIEEVEGLGGMAKAIEAGIPKLRIEEAA
                     AKTQARIDAGVQSVIGVNKYPPSDETPIDVLKIDNSAVRASQINKLKRLKAERDPSAL
                     EEALDALTRAADRGNGNLLALAVDAARAEATVGEISSALEKVFGRHRAEIKAITGVYK
                     REVGMTGAVERVRTAVEGFESAEGRRPRLLVAKIGQDGHDRGQKVIASAFADLGFDVD
                     IGPLFATPAEAARQAVENDVHILGVSSLAAAHLTLVPELKAELKKQGREDIMIVVGGV
                     VPPQDYDALMKAGAEAIFPPGTVIAEAAEKLLKKLNKRLGHSEEAAE"
     gene            1317..1862
                     /locus_tag="VFL49_05810"
     CDS             1317..1862
                     /locus_tag="VFL49_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911524.1"
                     /translation="MTPSYTCVAGAVLCALSLSGCVDSSGPILPDARPVFGEQLRLQF
                     YSLRKGIADEPEQATYKWDGATYLRTGGGMTDVGSFSVHPLARDTYVVQSAAAKRPGT
                     FEYAIARRLADGVYQVTAIDEADAGRVTRARYCKRTSDSSCRIERRNQLYAFARATAE
                     RRRGQGGLVLRLADGVAESSR"
     gene            1892..2878
                     /gene="meaB"
                     /locus_tag="VFL49_05815"
     CDS             1892..2878
                     /gene="meaB"
                     /locus_tag="VFL49_05815"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET7911525.1"
                     /translation="MASRNLPSADLVRGVRAGDRAMLARAITLIESKRADHRKTAHHL
                     VQELLPLTGKGVRLGITGAPGAGKSTTIDVLGTYLTGKGHKVAVLAVDPSSTRTGGSI
                     LGDKTRMAQLASDAHAFVRPSPSSGTLGGVAAKTRETMLVCEAAGYDVVMVETVGIGQ
                     SETAVADMTDFFLVLMLPGAGDELQGLKKGIVELADMVAVNKADGDNVERARVAAAEY
                     KAALNILSPQSATWAPPVITYSARTGGGIAELWAQVIAHKEKMTASGELAARRREQQV
                     KWMWTMLEERLTARLRSDASVRSKLRQMEAQVAAGKLAPTLAVEEIAKLLGV"
     gene            2879..3508
                     /locus_tag="VFL49_05820"
     CDS             2879..3508
                     /locus_tag="VFL49_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET7911526.1"
                     /translation="MPLTILVTGFGPFPGAPFNPTGPLAQRLTRARRPALAGVKIVTH
                     VFPTSYAAVDRELPPLIARHKPDALLMFGLAPRARELRVETRARNALALLPDARGASL
                     GRRAIAVDGPTSMKMQTPARSLLAALRGLRPRATLSRDAGRYLCNYLCWHATEAALAG
                     TGPRFVTFIHVPPVRRGPRRKRDKVLAGLNDLTVAGTRALVALVAAARR"
     gene            3535..4866
                     /locus_tag="VFL49_05825"
     CDS             3535..4866
                     /locus_tag="VFL49_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HET7911527.1"
                     /translation="MPMDRRSLLIGSLSGAAALTVPLSAYGIDAAQFGVRPGAPDDQS
                     RALQRAISQAARTRAPLMLAPGVYRTGDLKLPAGAQLFGVRGATRLLLTRGPSLLSGE
                     GGDHITISGLVLDGVGQPLPQNRGLVHLVASKSIRIVDCEFSGAGGNAIALERCEGRV
                     TANSITGAADNAIFCNDNFGVTISSNTIRDSGNGGIRVWQSVKRHDGSIVADNTIDDT
                     EARAGGTGENGNAINVFRAANVIVRNNIIRRAAFSAIRGNAAANIQIIGNNCASLKET
                     ALYSEFDFEGAIVANNVVDTAENGISVTNFNNGGRLATVHGNLIRNIGVQRPGTSPED
                     AGIGIGIEAETAVTGNVVESAAHIGIRAGWGPYLRNVTISGNVVRNAAIGIAVSVAPK
                     AGDAIVSGNIIAGAQLGAVVAMEWSKVVSGDLAKEGAARYPQLAIDNNQTR"
     gene            4908..5693
                     /locus_tag="VFL49_05830"
     CDS             4908..5693
                     /locus_tag="VFL49_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HET7911528.1"
                     /translation="MTPTQSPRRRLRTVMFAILLLFIAPILARVVLYAISDDPRSWRD
                     ADWSATGLLPAAADFAPARVIIFTGTAGAWKGVFSVHSWIALKHANASRWQRYDVVGW
                     GQPVRLNNWPVDGRWYGNVPVMLADISGLEAEKLIPRIEAAVKDYNYSQTGDYRIWPG
                     PNSNSFIAAILRSVPELELALPSNAVGRDFRYGFYAGQTDSRTGFEINLHGVAGLKVG
                     WVEGVEVNLLGLVAGLDLRHPGLKLPGFGRIGIDLPVTTALAR"
     gene            5791..6762
                     /gene="arsS"
                     /locus_tag="VFL49_05835"
     CDS             5791..6762
                     /gene="arsS"
                     /locus_tag="VFL49_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HET7911529.1"
                     /translation="MRDTLPLLEKIPFPTVRRGKLEILQVNVGYRCNQACFHCHVNAG
                     PNRTEEMNGDIADLVLKFLRHRKIATLDITGGAPELNPNFRRLVQDARKSGAKVMDRC
                     NLTILEQPGQEDLAAFLAAEHVEVVASMPCYLQDNVERQRGKGVFDASIRGLMRLNSL
                     GYGRDPELALNLVYNPQGPSLPPAQASLEADYKRVLGERYGIEFNKLFVLANMPIQRF
                     GTTLITNGEFNAYIELLRDAHLDANLDGVMCRSLISVDWRGYVYDCDFNQMLQLPMAR
                     GSRSVTHLSDLLDDPINGNPVHVAGHCYGCTAGQGSSCGGALKEAAE"
     gene            6759..7457
                     /locus_tag="VFL49_05840"
     CDS             6759..7457
                     /locus_tag="VFL49_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET7911530.1"
                     /translation="MNDPFLSIVVPVLNEAAQIEAMFKLLSPFRERGAEVIVVDGGSS
                     DATADVARTHAHHVLTSTQGRATQMNAGAAIARGKVLLFLHADTQLPDGADRLILAGL
                     EATGRNWGRFDVRFNEGPLLGLIALMMNLRSKMTGIATGDQAIFVSRAVFEAVGGFPS
                     IALMEDIALSAHLKRIGRPLCLRARVTTSPRRWKENGTLRTVFLMWRLRLAYFLGADP
                     RQLAQRYGYVPAEQ"
     gene            7432..>7917
                     /locus_tag="VFL49_05845"
     CDS             7432..>7917
                     /locus_tag="VFL49_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET7911531.1"
                     /translation="MAMSPPSNSPVSIAILAKAPIAGYAKTRLIPAIGAHAAAVLQER
                     LTERTVTTAIASGVGPVILCCAPDATHDSFLKMVARLRITLRPQPQGDLGARMLAAAA
                     GSAGPVLIIGTDCPALTEMHFRSAAAALHDGADVVLIPAEDGGYVLLGMRKAQPALFS
                     NI"
BASE COUNT         1513 a         2495 c         2407 g         1502 t
ORIGIN      
        1 ggcatgaact atttcatgga gatcgcgaag ttgcgcgccg cccgtatgct ttgggcaaag
       61 ctgatgaagc ccttcaaacc ggaggacgta cgttctctct ccctgcgcac gcattgccag
      121 acgtcgggct ggtctcttgc cgcacaggac gtattcaaca acgtcgcccg taccatgatc
      181 gaggcgatgg cggcgacgca aggccagaca cagtcgctgc acaccaacgc gctcgacgag
      241 gcgcttgcct tgccgactga cttttcagcg cgtatcgcgc gcaataccca gatcgtgctg
      301 cagcaggagg ccggggcaac ccgcattatc gatccgtggg gcggctccta ttacgttgag
      361 cggttgacgt atgaactcgc acgcaaggcc tggggccata tcgaggaggt cgagggtctc
      421 ggcggcatgg ccaaggcaat cgaagcggga attccgaagc tccgcatcga agaggcagcc
      481 gcgaagacgc aggcccgcat cgatgcaggc gtgcagtccg tgatcggggt caacaaatat
      541 ccaccatcgg acgagactcc gatcgacgtg ctcaagatcg acaattcggc cgtgcgggca
      601 agccagatca acaagctaaa gcggctgaag gccgagcgcg atccgtcggc cctggaggag
      661 gccctcgatg cgctgacacg cgctgcagac cgcggcaacg gcaacctgct cgcccttgcc
      721 gtcgatgccg cgcgagccga ggcgaccgta ggcgagattt catcggccct ggaaaaggtc
      781 ttcggccgcc accgcgccga gatcaaggca atcacagggg tctacaagcg ggaggtcggc
      841 atgaccggcg ccgtcgaacg tgtccgcact gccgttgagg gttttgaatc agccgaaggc
      901 cggcgcccgc gtctgcttgt cgccaaaatt ggccaagacg ggcacgatcg cggccagaaa
      961 gtgatcgcct cggcattcgc cgacctcggt ttcgatgtcg atatcggtcc gctgtttgcc
     1021 acgcccgccg aggcggccag acaggctgtc gagaacgacg tccatatcct tggcgtgtct
     1081 tcccttgcag ccgcccattt gacgctcgtg cccgagctga aggccgaact caagaaacag
     1141 ggtcgcgagg acatcatgat cgtcgtcggc ggggtcgtgc cgccgcagga ctatgacgcc
     1201 cttatgaagg caggcgcgga agcgatcttt cctcccggca cggtgatcgc cgaggcggcg
     1261 gaaaagctgc tcaaaaaact gaacaagcgg ctcggacaca gcgaagaggc tgcggagtga
     1321 cgccgtctta cacctgcgtc gccggcgcgg tcctttgcgc attgtcattg tcgggctgcg
     1381 tcgattcgtc gggaccgatt cttccggacg cgcggcccgt ctttggcgaa cagttgcggc
     1441 ttcagttcta ctcgctgcgc aagggaatcg ccgacgagcc cgaacaggca acctataaat
     1501 gggacggtgc gacgtatctg cggacgggcg gcggcatgac ggacgttggc tcgttctcgg
     1561 ttcacccgct ggcacgcgac acctacgtcg tacagagtgc agccgctaag cgacccggca
     1621 cgttcgaata tgcgatcgcg cgcaggcttg cggatggggt ttaccaggtg accgcaatcg
     1681 atgaagccga tgccggccgg gtgacacggg cccgttactg caagcgcacc agcgactcgt
     1741 cttgccgtat cgaacggcgc aatcagcttt atgcttttgc ccgcgcgacg gccgagcgga
     1801 gaaggggcca aggcggcctc gtgcttcgtc tcgcggatgg cgttgcggaa tcctcgcgtt
     1861 agcatgatca gtcccggggc ttgaggactc gatggcttcc cgcaacctgc catctgccga
     1921 tctcgttcgc ggcgtgcgtg ccggcgaccg cgcgatgctt gcccgcgcga tcacgctgat
     1981 cgagagcaag cgtgcggacc atcgcaagac cgcacatcat ctggtgcagg agctgctgcc
     2041 cctcaccggc aaaggcgtgc gcctcggcat caccggcgcg cccggcgccg gcaaatcgac
     2101 cacgatcgat gtcctcggaa cctacctgac cggcaagggc cacaaggtcg cggtcctcgc
     2161 cgttgatccg tcctcgacac gaaccggagg ttccatcctc ggcgacaaga cgcgcatggc
     2221 gcagcttgcc agcgatgcgc atgccttcgt gcgcccgtcg ccttcgtccg gcacgctcgg
     2281 cggtgtcgcg gccaaaacac gtgagaccat gctggtttgt gaggccgccg gctacgacgt
     2341 ggtgatggtg gaaacggtcg gcatcggtca gtcggagacg gcggtcgccg acatgactga
     2401 ttttttcctg gtgctgatgc tgccgggcgc cggcgacgaa ttgcagggcc tgaagaaagg
     2461 catcgtcgag cttgccgaca tggtcgccgt gaacaaagcg gacggcgaca atgtcgaaag
     2521 ggcaagggtc gccgcggcag agtataaggc cgcgctcaat atcctttcac cgcagtctgc
     2581 aacatgggcc ccacccgtca ttacctactc cgcgcggacc ggcggcggca tcgccgaatt
     2641 gtgggctcag gtcatcgcac acaaagagaa aatgacggca tcgggcgagc tggcggcgcg
     2701 tcggcgtgaa cagcaggtga aatggatgtg gaccatgctg gaggaacgcc tgaccgcgcg
     2761 cttgcgcagc gacgcttctg tacgctcgaa gctgcgccag atggaggcgc aggtcgccgc
     2821 cggcaagctc gcgccgacac tcgccgtcga agagatcgcg aaattgcttg gcgtctagat
     2881 gccccttacg atcctcgtca ctggatttgg gccattcccg ggtgcaccgt tcaacccgac
     2941 cggtcccctc gcccaacgac ttacgcgagc gcgccgcccc gctttggcag gcgtgaaaat
     3001 cgtcacgcat gtatttccca caagctatgc ggcagtcgac cgtgaattgc cgccgttgat
     3061 tgcccggcac aagccggatg cattgctgat gttcggactt gcgccgcgtg cgcgcgagct
     3121 tcgcgtggaa acccgcgcac gaaatgcttt ggcgctcttg cccgacgctc gcggcgcatc
     3181 gttgggacga cgggccatcg cggtcgacgg gccgacatcg atgaaaatgc agacgcccgc
     3241 ccgctcgctg cttgccgcgc ttcgcggcct gcggcctcgc gccacacttt cacgcgatgc
     3301 ggggcgatat ctctgcaact acctttgctg gcacgccacc gaggcggcat tggcaggcac
     3361 cgggcctcgt ttcgtcacct tcattcatgt accgccggtc cggcgcggcc cgcgacgaaa
     3421 gcgtgacaag gtattggcag gcctcaatga cctgaccgtc gccgggaccc gcgcgcttgt
     3481 cgctctcgtc gcagcagcac ggcgttaacc tccccggcgc atcatgtgcg cgccatgccg
     3541 atggaccgcc gcagcctgct gattggttcg ctctcgggcg ccgccgcatt gaccgtgccg
     3601 ctttcggcct atggaattga cgccgcacaa ttcggcgttc ggcctggcgc tcctgacgat
     3661 caaagccgtg ccctgcaacg cgctattagt caggcggcgc ggacgcgcgc cccgctgatg
     3721 ctcgcgcctg gtgtctatcg cactggcgat ctcaagcttc cggccggggc gcagcttttc
     3781 ggcgtgcgcg gtgccacccg cctcttgctt acccgcggtc cgtcattgct gtcaggcgaa
     3841 ggcggagatc acatcacgat ctccggcctg gtgctggacg gcgtcgggca gccgctgccg
     3901 cagaaccgcg gcctggtaca cctcgttgca tcaaagtcga tccgcatcgt cgattgcgaa
     3961 ttttccggcg ctggcggaaa cgcgattgcg ctcgaacgtt gcgagggacg ggtcacagcc
     4021 aattcgatca cgggtgcggc ggacaatgcg attttctgca acgacaattt tggcgtcacc
     4081 atctcttcca ataccatccg ggattccggc aatggcggca tccgggtctg gcaaagcgta
     4141 aaacggcacg acggcagcat cgtcgccgac aacacgatcg acgacaccga ggcgcgcgcc
     4201 ggcggcaccg gagagaacgg caatgcgatt aacgtcttcc gggctgccaa cgtaatcgtg
     4261 cgtaacaaca tcattcgccg cgcggcgttc tccgccattc gcggcaacgc ggcggccaat
     4321 atccagatca tcggcaacaa ttgcgcatca ctgaaggaaa ccgcgctgta ttcggaattc
     4381 gatttcgaag gtgcgatcgt tgccaataac gtggtcgata cggcggaaaa cggaatctca
     4441 gtcacaaatt tcaacaatgg cgggcggctg gcgaccgttc atggcaatct tattcgcaac
     4501 atcggcgtgc agcgcccagg cacgtctccc gaagatgccg gcatcggcat aggtatcgag
     4561 gcggaaaccg cagtgaccgg aaacgtggtc gagagtgccg cccatatcgg aatacgagcc
     4621 ggctgggggc cttaccttcg caatgtgaca atcagcggca acgttgtccg caacgccgcc
     4681 atcgggatcg ccgtatctgt cgcgcccaag gccggtgatg ccatcgtctc cggcaatatc
     4741 attgccggag cgcaacttgg cgccgtcgtc gccatggaat ggtccaaggt cgtatcgggc
     4801 gatctcgcca aggaaggcgc cgcacgctat ccgcagctcg ccatcgacaa caaccaaacg
     4861 cggtaacagc cacgtttagg ccggaaagcg tctataattc cgcaatcatg acgcccaccc
     4921 agtcaccacg acgacgcctc aggaccgtaa tgttcgccat ccttcttctt tttatcgcgc
     4981 cgatcctcgc ccgtgtcgtc ctgtacgcga ttagcgacga tccgcgctcg tggcgcgatg
     5041 ccgactggtc ggccacggga cttttgccgg cagcagctga ttttgcgccc gcacgcgtaa
     5101 tcatattcac cggcaccgcc ggcgcctgga aaggcgtttt ctcggtgcac agttggatcg
     5161 ccctcaagca cgcgaatgcg tcacgttggc aacgttatga cgtggtcgga tggggtcagc
     5221 cggttcggct caacaattgg ccggtcgacg gcagatggta tggcaacgta ccggttatgc
     5281 tcgccgatat ttccgggctc gaggctgaaa agcttatccc gcgcatcgaa gccgctgtta
     5341 aagactacaa ttactctcaa accggcgatt accgaatctg gcccggtccg aacagcaaca
     5401 gcttcattgc cgcgatcctg cgtagcgtgc cggagctcga actggcattg ccctcaaatg
     5461 cggtcggccg cgatttccgt tacggctttt acgccggaca aaccgacagt aggaccggtt
     5521 tcgaaatcaa tctgcacggt gtcgccggac tgaaggttgg ctgggtcgag ggtgtcgaag
     5581 tcaatctcct gggcttggtt gcaggtctcg atctgcgtca tccgggactg aagttgccgg
     5641 gcttcggacg gatcggcatt gatttgcctg tgacgacagc gctggcccgc tgaaatctca
     5701 ccactcacga cgatttgtcc agcgatcaat atcccggggt gacgtattcg cggccttcgt
     5761 ggcacattgc cggtggaatt gaggccgcca atgcgcgaca cgctgccgct gctcgaaaag
     5821 attccctttc ccacggtccg ccgcggcaag ctcgaaatct tgcaggtcaa tgtcggctat
     5881 cgctgcaatc aggcctgctt ccattgccac gtcaatgccg gtccaaacag aaccgaggag
     5941 atgaacggcg acattgccga tctcgttctg aaatttctcc gtcacagaaa aatcgcgaca
     6001 ctcgatatca ccggcggcgc gccggagctc aacccgaatt tccgccgtct ggtgcaggac
     6061 gcgcgcaagt caggcgcaaa ggtcatggac cgctgcaacc tcacaatact ggagcagccc
     6121 gggcaggaag acctcgcagc atttctcgcg gcagagcacg ttgaggtggt tgcatccatg
     6181 ccctgttact tgcaggacaa cgtggaacgt cagcgcggta aaggcgtctt tgacgcatcg
     6241 atccgtggtc ttatgcggct taattcactt ggatatgggc gcgacccaga actggcgctc
     6301 aatctcgtct acaacccgca ggggccgtca cttccgccag cgcaggcctc gctcgaggcc
     6361 gactacaaac gcgttcttgg cgaacgttat gggatcgaat tcaacaagct ctttgtgctt
     6421 gccaatatgc cgatccagcg ctttggcacc acactgatca ccaacggcga attcaacgct
     6481 tacatcgaat tgttgcgcga cgcgcatctc gacgccaacc tcgacggcgt catgtgccgg
     6541 agcctcatat cggtcgattg gcgcggctat gtctatgact gcgatttcaa tcagatgctt
     6601 cagctgccga tggcgcgagg ctcgcgttca gtcactcacc tctcagatct tctcgatgac
     6661 ccgatcaatg ggaatccggt ccatgtcgcc ggccattgct acggctgcac cgccggacag
     6721 ggttccagct gcggtggcgc gttgaaagag gcggcggaat gaacgaccca tttttgtcaa
     6781 ttgtcgtccc tgtcctgaac gaagccgcgc aaattgaggc catgttcaaa ttgctttcgc
     6841 cgtttcgtga aaggggtgcc gaggtgatcg tcgtcgatgg tggaagcagc gacgctacag
     6901 cggatgttgc tcgcacgcat gcccaccacg tccttacaag cacgcagggc cgggccaccc
     6961 aaatgaatgc aggcgcagca atagcgcgcg gcaaagtctt gctgtttctg cacgccgaca
     7021 cgcaactgcc ggacggcgct gaccggctca tcctcgcagg cctcgaggca acaggccgca
     7081 actggggccg atttgatgtg cgatttaacg agggacccct gctcggcttg atcgccctga
     7141 tgatgaattt gcgatcgaag atgaccggca ttgccacggg cgatcaggcc atcttcgtgt
     7201 ctcgtgctgt ctttgaggca gttggcggct ttccatccat tgcacttatg gaggacatcg
     7261 cgctttcggc acacctgaaa cgtatcggcc gtcctttgtg cctccgcgcg cgggtgacga
     7321 catcgccgcg ccggtggaaa gaaaacggga ctttgcgcac agtcttcttg atgtggcggc
     7381 tgcggctggc gtatttcctc ggcgccgacc cgaggcaact cgcgcaacgc tatggctatg
     7441 tccccgccga gcaatagtcc cgtatcgatt gcaatattgg cgaaggctcc gatcgcgggc
     7501 tacgccaaga cacggctcat tcccgcgatc ggcgcgcatg ccgcggcggt cctgcaggag
     7561 cgcttgaccg aacgtacggt cacgaccgcg atcgcctcgg gcgtcggccc ggtcattttg
     7621 tgctgcgcgc ccgacgccac gcatgactca tttctcaaaa tggtcgcgcg gcttaggatc
     7681 acgctcaggc cgcaaccgca gggcgacctt ggtgcacgga tgcttgccgc ggctgccggc
     7741 agcgccggac ccgttctgat aatcggcacc gactgccccg ccctcacaga aatgcatttt
     7801 cgcagcgccg ccgcggcact gcacgacgga gccgacgttg tcctgattcc cgctgaggac
     7861 ggcggctacg tccttctcgg gatgcgcaag gcacaaccgg cgctcttctc caatatc
//
LOCUS       DASSRL010000283         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1127491, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000283 DASSRL010000000
VERSION     DASSRL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127491"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="eno"
                     /locus_tag="VFL49_05850"
     CDS             <1..436
                     /gene="eno"
                     /locus_tag="VFL49_05850"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503221.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7911532.1"
                     /translation="ISIEDGMAEDDWEGWKSLTMLLGNKCQLVGDDLFVTNVTRLSKG
                     IKEGVANSILVKVNQIGTLTETLAAVEMAHKAGYTAVMSHRSGETEDSTIADLAVATN
                     CGQIKTGSLARSDRTAKYNQLLRIEEELGPQAKFAGRDILKA"
     gene            514..1317
                     /locus_tag="VFL49_05855"
     CDS             514..1317
                     /locus_tag="VFL49_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911533.1"
                     /translation="MDQTTTLSTYDRRRASDRLATFIVQSIDAARAVEKPFFHLEFDR
                     VFPDDTYAQILALMPESSDYRPMHGRSKGHDLADGTHTRVKIDLFPEYIRNLPTEKRA
                     LWDVVGRALCSEPVKLAFIRRLAPGLSKRFGESFAKVGMYPIPILTRDIPGYLITPHT
                     DTRWKGITVQLYLPKDDANTDIGTIFHEKLPDGSLPKKAQMRFAPNSGYAFAVGSDTW
                     HSADPVHSRVKTRDSILLTYFVDQGVLKVLRNRGKRVGNFFLNEIRHRI"
     gene            1361..1678
                     /locus_tag="VFL49_05860"
     CDS             1361..1678
                     /locus_tag="VFL49_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7911534.1"
                     /translation="MVTHRRRRTFLTVLGLYIFAALFIGYFAANAFSGNHGLRAQQDL
                     EQQLTAMKAELAGLKAERAVWERRVSLLRADRIDPDMLDERARALLGYADPRDLTLLL
                     NPR"
     gene            1865..2872
                     /gene="pdhA"
                     /locus_tag="VFL49_05865"
     CDS             1865..2872
                     /gene="pdhA"
                     /locus_tag="VFL49_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET7911535.1"
                     /translation="MAVAQKNPSADAKPVADFSKEQELSAYRTMLTIRRFEEKAGQMY
                     GMGLIGGFCHLYIGQEAVVVGMQMVLKPGDQVITGYRDHGHMIATGMDPKGVMAELTG
                     RAHGYSKGKGGSMHMFSVEKGFYGGHGIVGAQVPLGTGLAFANNYRSNGNISITYFGD
                     GAANQGQVYESFNMAELWKLPVIYVIENNRYAMGTAITRSSAEVNLSRRGLSFNIPGD
                     QVDGMDVRAVKAAGEKAAAWCRAGKGPYILEMLTYRYRGHSMSDPAKYRTREEVDEVR
                     SHHDPIDMARHRILEKKFASEDALKKIDGEVRGLINQAAEFATHDPEPDVSELYTDVY
                     R"
     gene            2898..>3823
                     /locus_tag="VFL49_05870"
     CDS             2898..>3823
                     /locus_tag="VFL49_05870"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HET7911536.1"
                     /translation="MPIEVLMPALSPTMEKGNLAKWLKKEGDEIKSGDVIAEIETDKA
                     TMEVEATDEGTLGKILVPEGTADVAVNTPIAVILGEGEDEAAIKSPKPHQQQKAAESA
                     PPAKMQKPQAKAQDAPAPKPAKAPDAPAPQAADDPDIPEGTEMVTITMREALRDAMAE
                     EMRRDKDVFVMGEEVAEYQGAYKVTQGLLQEFGATRVIDTPITEHGFAGLGIGAALAG
                     LKPVVEFMTFNFAMQAMDQLINSAAKTLYMSGGQMGCQIVFRGPNGAAARVAAQHSQD
                     YSAWYSHIPGLKVIAPSTAADAKGLLKAAIRDP"
BASE COUNT          816 a         1104 c         1187 g          716 t
ORIGIN      
        1 catctccatc gaggacggca tggcggagga tgactgggaa ggctggaaga gtcttacgat
       61 gctgctcgga aacaagtgcc agctcgtagg cgacgatctt ttcgtgacga acgtgacgcg
      121 cttgtcgaag ggcatcaagg agggcgtcgc aaactcaatc ttggtgaaag tcaatcagat
      181 cggcacgctc acggagacgc tggctgcggt cgagatggct cacaaggccg gctacaccgc
      241 ggtgatgtca catcgttcgg gtgagacgga agattcgacc atcgccgacc tcgccgttgc
      301 tactaattgc gggcagataa agaccggatc acttgcgcgg tccgaccgaa ccgcaaaata
      361 caatcagctg ctgcgtatcg aggaggagct cggtccacag gcaaaattcg ccggccgcga
      421 tatcctgaaa gcctgagtag cgttctggca tgtcttgcct ttcgtcgtga atttgacgca
      481 aattgcttcc ggaccagatt gtgggtgtgc acgatggatc agaccacgac cctgtcgact
      541 tatgaccgtc gccgagcctc ggaccgcctg gcaacattta tcgtgcagtc gatcgatgcg
      601 gcacgcgcgg tcgagaagcc gttttttcat ctcgaattcg acagagtgtt ccctgacgac
      661 acctacgcgc aaatcctcgc gctgatgccg gagtcatcgg attaccggcc aatgcacggg
      721 cgcagcaagg gacatgatct cgccgatgga acacacacgc gggtgaagat tgatctgttc
      781 ccggaataca tccgcaacct gccgacggaa aagcgcgcgc tgtgggacgt cgtcggccgc
      841 gccctttgtt cggaaccggt caagctggcc tttatccggc gcctggcgcc tgggttgtca
      901 aaaaggttcg gcgaaagctt tgcgaaggtc gggatgtatc ccattccgat tttgacgcgc
      961 gacattcccg gctatctcat cacaccgcac acggacacgc gttggaaggg catcactgtt
     1021 cagctctatc tgccgaagga cgatgccaac accgacatcg gcacgatctt tcacgaaaag
     1081 ctgcctgacg gctccttgcc gaagaaggcg cagatgcgtt ttgcgccgaa tagcggatat
     1141 gcgtttgctg tcggaagcga cacctggcat tccgccgacc ccgttcatag ccgcgtgaag
     1201 acgcgcgatt cgattctgct cacttatttt gtggatcaag gcgtgttgaa ggtcctgcgc
     1261 aaccgcggca agcgcgtcgg aaacttcttc ctcaacgaga taaggcaccg gatctgaggc
     1321 attaaaggcg ccttaacggc cttggtccat gttgcgcgtc atggtcacgc atcgccgccg
     1381 ccgcacattc ctgaccgttc tcggtctcta catttttgcg gcgctgttca tcggctattt
     1441 cgcggccaac gcgttcagcg gcaatcatgg cttgcgggcg caacaggatc tcgagcagca
     1501 gctgacggcc atgaaggccg agttggcggg gctcaaggcc gagcgcgcgg tctgggagcg
     1561 gcgcgtctcg ctgttgcgtg ccgaccggat cgatcccgac atgttggacg agcgggcgcg
     1621 cgcgctgctc ggctatgcgg atccgcgcga tctcaccttg ctgctcaatc cccgttagca
     1681 accgcaaggg cctggcggcg ttatccaccg gccgccttga aattttcagc caatttccgg
     1741 gcgttttgcg caatggcccg cggcagtctc gcgctttcgc cgccgagcaa tttaagctat
     1801 tggggttcgg gaaagcctac cgacatcccg tcacaactgc cggacgcttt cgcagggagt
     1861 gccaatggcg gtcgcccaga aaaacccgag cgccgacgcc aagcctgttg ccgatttcag
     1921 caaggagcag gagctatccg cctaccgcac catgctgacc attcgccgct tcgaggagaa
     1981 ggccgggcaa atgtatggca tgggtctgat cggcggcttc tgccacctct atatcggaca
     2041 ggaagcggtc gtcgtcggca tgcagatggt gctcaaaccg ggtgaccagg tcatcaccgg
     2101 ctatcgcgac cacggccaca tgatcgcgac cggcatggac ccgaaagggg tcatggccga
     2161 gctgaccggc cgcgcccatg gctattcaaa gggcaagggc ggctccatgc acatgttcag
     2221 cgtggagaag ggcttctacg gcggacacgg aattgtgggc gcgcaggtac cgctcggaac
     2281 gggactcgcg ttcgccaaca actatcgaag caacggcaat atcagcatca cttacttcgg
     2341 cgacggcgcc gccaatcagg gccaagtcta cgaaagcttc aacatggcgg agttgtggaa
     2401 gctgccggtg atctacgtga tcgagaataa tcgctacgca atgggtacgg cgatcacgcg
     2461 atcttcagcg gaagtcaacc tgtcgcgtcg gggattgtcc ttcaacattc cgggtgacca
     2521 ggtcgacggc atggatgtgc gtgcggtgaa agcggccgga gaaaaggctg ccgcatggtg
     2581 ccgggccggc aagggcccgt acatcctcga aatgctgacg taccggtatc gcgggcactc
     2641 aatgtcggat cctgcgaagt accgtacgcg cgaagaagtc gacgaagtgc gcagccatca
     2701 cgatccgatc gacatggccc ggcacagaat tctggaaaag aaattcgcat ccgaagatgc
     2761 attgaagaag atcgacggtg aagttcgcgg cctgatcaat caggcggcgg aatttgccac
     2821 gcacgatccg gagcccgatg tctccgaact ttataccgat gtgtaccgct gatttgttgt
     2881 ctggaaaccg ataggccatg ccgattgaag tgctcatgcc cgcgctctcc ccgacgatgg
     2941 agaagggcaa tctggccaag tggctcaaga aagagggcga tgagattaag tccggcgatg
     3001 tgatcgccga gatcgagacc gacaaggcca cgatggaggt cgaggcaacg gacgaaggta
     3061 cgctcggcaa aattctcgtc cctgagggaa cagccgacgt tgcggtcaat acgcctatcg
     3121 ccgttattct cggcgaaggc gaggacgagg cggcgatcaa gtcgcccaag ccgcatcagc
     3181 aacagaaggc ggctgagtcg gcgccgccgg cgaaaatgca gaagccgcag gccaaggcac
     3241 aggatgcgcc agcgcccaag ccggcgaagg cgcctgacgc tcccgcaccg caggcagcgg
     3301 atgatccgga catcccggaa ggcaccgaga tggtcaccat caccatgcgc gaggcgctgc
     3361 gtgatgcgat ggccgaggaa atgcgccgcg ataaagatgt tttcgtgatg ggtgaagagg
     3421 tcgccgagta tcagggcgca tacaaggtga cccaggggct gctgcaggag ttcggcgcga
     3481 cgcgggtgat cgatacaccg atcaccgaac atggttttgc cggcctcggc atcggtgccg
     3541 cgctcgccgg cctcaagccg gtcgtcgagt tcatgacttt caacttcgcc atgcaggcga
     3601 tggaccagct catcaactcg gcggcgaaga cgctctatat gtcgggcggg cagatgggct
     3661 gccagatcgt gttccgtggc ccgaatggcg cggccgcgcg tgtggctgcc cagcacagcc
     3721 aggattattc ggcgtggtat tcgcacattc cgggattgaa ggtgattgcg ccatcgacgg
     3781 cagccgatgc caagggatta ctgaaggcgg caatccgcga ccc
//
LOCUS       DASSRL010000284         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_199811, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000284 DASSRL010000000
VERSION     DASSRL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199811"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VFL49_05875"
     CDS             <1..1519
                     /locus_tag="VFL49_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HET7911537.1"
                     /translation="KSIEHSMTMSGAIPTYLVPTRNRLGLIGPIPQDRLMPNAIKKAI
                     KDNPLVGKDVDPTPVHAVITNSTYDGLCYSAKRVKELLGQSVDRLHFDEAWYGYARFN
                     PLYRDRHAMYGSPTDHPATDPTVFATHSTHKLLAALSQASYIHIRDGRRPIPHERFNE
                     AFMMHASTSPQYAIIASNDVSAAMMDGPSGIALTTESIREAVAFRQMLARLHDDFAKK
                     NEWFFTAWQPTTVQDQKTGKSVPFHQAAEQQLVTDPSCWVLHPKQNWHGFDGLEDDYC
                     MLDPIKVSIVTPGVDADGTLAKTGIPATLVTAYLHQQGIEVEKTTDFTILVLFSLGIT
                     KGKWGTLVNALLDFKTDYDANKPLERTLPQLAAKTGNAYAGVGLKDLAARLFEAMRKL
                     KTTEYLAKAFSLLPKASVSPVQAYETLVKGEVETLTLDKIAGRSVATGIVPYPPGIPL
                     LMPGEEVGAADGPILGYLKSLEAIDRLVPGFGHDTHGVENHEGVYHLQCLRSSGK"
     gene            1539..2888
                     /gene="potE"
                     /locus_tag="VFL49_05880"
     CDS             1539..2888
                     /gene="potE"
                     /locus_tag="VFL49_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HET7911538.1"
                     /translation="MAASKSKMNVWQLTILTAVNMMGSGIILLPAKLAEVGTISIFSW
                     LITAGGSLALAYAFARCGMLSKKPGGMGGYAEYAFGKGGNYMANYTYGLSLVIGNVAI
                     GVTAVGYGTVLFGTSLTPIQTCIWTIILLCLTTFANFGGAQITGRIGSVTVWGVIIPT
                     IVISIIGWFWFSAGTYAAAWNPHDLPFMTAVGSAISITLWAFLGLESACANADAVENP
                     ERNVPIAVLGGTIGAAIIYIVSTNVIAGIVPNADLAASSAPFGLAYATMFNATVANIV
                     TGLMVVACIGSLLGWQFTVAQVFKSSADVGYFLPVFGKATAIGVPIIGMLILLAAQLA
                     LALMTISPELNEQFGRVVNLAVVTNLVPYVMSMAALITIQNVSGVPRGKALLTNIIAM
                     VATIYSFYALWSSGTEAIMLGGLCIFLGWTLFGFVSARFYRMEVAAHVANPGKSLQA"
     gene            2976..3392
                     /locus_tag="VFL49_05885"
     CDS             2976..3392
                     /locus_tag="VFL49_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911539.1"
                     /translation="MNVRTMLFATGVGLVLSAMPAYAGPCSLEIETLAQALPGAGSHL
                     PKAPSTTGHVPDNPLDTTIHGGNLAAAGAMPKAPSTAGTPPANPLSSSSGANGATETG
                     ATASASLQRARELDQVGDEAGCMNEITKAKQLLGTD"
     gene            complement(4100..>4376)
                     /locus_tag="VFL49_05890"
     CDS             complement(4100..>4376)
                     /locus_tag="VFL49_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911540.1"
                     /translation="AAGDPAIGAAIGGGSGLLVGSSAGVSNAYASGAALQSRYDMAYT
                     QCMSAKGESVPMAHNGSASYPPLYAYRMYPAYPGYYYPGYYYYPYPY"
BASE COUNT          872 a         1382 c         1313 g          809 t
ORIGIN      
        1 caaatcgatc gagcattcca tgaccatgtc gggtgccatc ccgacctacc tggtgccgac
       61 ccgcaatcgc ctcggcctga tcggcccaat cccgcaggac cgcttgatgc cgaatgccat
      121 caagaaagcg atcaaggaca atccactggt cggtaaggat gtcgatccga ccccggttca
      181 tgccgtcatc accaactcga catatgatgg tctctgctac agcgccaaac gggtcaagga
      241 gctgctgggg cagagcgtcg accggctgca tttcgatgaa gcctggtacg gctatgcgcg
      301 attcaacccg ctctatcgcg accggcacgc catgtacggc tccccgacgg atcacccggc
      361 caccgatccg acggtgttcg caacccactc gacccacaag ctgctcgctg ccctgtcgca
      421 ggcctcctac atccacatcc gcgatggtcg ccgaccgatc ccgcacgagc gcttcaacga
      481 ggcgttcatg atgcatgcct cgacctcgcc gcaatatgcg atcattgcgt ccaatgatgt
      541 cagcgcggcg atgatggatg ggccgtccgg cattgcgctg accaccgaat ccatacgcga
      601 agcggtcgcg ttccgccaga tgctggcgcg gctgcacgac gatttcgcca agaagaacga
      661 atggttcttc accgcctggc agccgacgac cgtgcaggac cagaagaccg gcaagtccgt
      721 tcccttccac caggcagcgg aacagcagct ggtcaccgat cccagctgct gggtgctgca
      781 tcccaagcag aattggcacg gcttcgacgg actcgaagac gattactgca tgcttgatcc
      841 gatcaaggtc tcgatcgtga ccccgggagt cgatgcggat ggcacgctgg cgaagaccgg
      901 catcccggca acattggtca ccgcctatct ccaccagcaa ggcatcgagg tcgagaagac
      961 caccgacttc acgatcctgg tgctgttctc gctcggcatc accaagggaa aatggggaac
     1021 gctggtcaac gccctgctcg attttaagac cgactacgac gccaacaagc cgctggagcg
     1081 cacgctgccg cagctcgccg ccaagacagg caacgcctat gcgggcgttg gcctaaagga
     1141 tctggccgcc cgcctgttcg aagccatgcg caagctgaag accacggagt atctggccaa
     1201 agccttttcg ctgctaccca aagccagcgt gagcccggtc caggcctacg aaacgctggt
     1261 aaaaggtgag gtcgaaaccc tgacccttga caagatcgcg gggcgcagcg tcgccaccgg
     1321 catcgtgccc tatccgccgg gcattccgct gctgatgcca ggcgaggaag tcggcgccgc
     1381 cgatggcccg atccttggtt atctgaagtc gctggaggcg atcgaccgcc tggtccccgg
     1441 cttcggccat gacacgcatg gcgtcgagaa ccacgaaggc gtctatcacc tgcaatgcct
     1501 gcgatccagc ggcaagtgac actcggaagg agcttcagat ggctgcgtcc aaaagcaaaa
     1561 tgaatgtctg gcagctcacc atcctgactg ccgtcaacat gatgggatcc ggcatcatcc
     1621 tgctgccggc caagctggcc gaggtcggca caatctcgat cttctcatgg ctgattaccg
     1681 ccggcggctc cctggcgctc gcctacgcgt tcgcgcgctg cggcatgctg agcaagaagc
     1741 ctggcggtat gggcggctac gccgaatacg cgttcggcaa gggcggcaac tacatggcca
     1801 actacactta tggcttgtca ctggtgatcg gcaacgtggc cattggcgtc acggccgtag
     1861 ggtatggcac cgttctgttc ggcacgagct tgacgccgat ccaaacctgt atctggacca
     1921 tcatcctgct ctgcctcacc accttcgcca atttcggcgg cgcgcaaatc accggtcgta
     1981 tcgggtcggt caccgtgtgg ggggtcatca taccgaccat cgtcatatcg atcatcggct
     2041 ggttctggtt cagtgccggc acctacgccg cagcctggaa tccacatgac ctgcccttca
     2101 tgacggcggt cggcagcgcc atctccatca cgctctgggc attcctgggc ctggaatcgg
     2161 cctgcgccaa cgcggacgcg gtcgagaatc ctgaacggaa cgtaccgatc gccgttctcg
     2221 gcggcaccat cggagccgcc atcatctaca ttgtttcgac caatgtgatc gccggcatcg
     2281 tgcccaacgc cgacctcgcg gcaagcagcg cgccatttgg cctcgcctac gcaacgatgt
     2341 tcaacgcgac ggtcgccaac atcgtcactg ggctgatggt cgtcgcctgc atcggctcgt
     2401 tgcttggctg gcaattcacc gtggcgcagg tcttcaagag ctctgccgat gtcggctatt
     2461 tcctccccgt cttcggcaag gcgacggcta tcggcgttcc gattatcggc atgctgattc
     2521 tgttagccgc gcaactggcc ctcgccctga tgacgatcag tccagagctc aacgagcagt
     2581 tcggcagggt cgtgaacctg gccgtggtca ccaacctggt gccgtatgtc atgtctatgg
     2641 ctgcactgat cacgatccag aacgtgtccg gcgttccgcg cggcaaggcg ctgctgacca
     2701 acatcatcgc gatggtcgcg acgatctaca gcttctatgc gctctggagt tcgggcacgg
     2761 aagccatcat gctgggcggt ctgtgcatct tcctgggctg gaccctgttc ggcttcgtga
     2821 gcgcccgatt ctaccggatg gaagtcgccg cgcacgtcgc gaaccccggc aaatcgttgc
     2881 aggcgtaggc tggtgcggtc cccctggagg cacgaccgat caggattgca ggcttgttga
     2941 ttccgctagg agcagctctc acgcggagga ttttcatgaa cgtcagaacc atgttgttcg
     3001 caaccggcgt cggattggtg ctgtccgcaa tgccagccta tgctggtcca tgcagcctgg
     3061 agatcgaaac cctggcgcag gcgcttccgg gcgccggctc gcatctgccg aaggcgccct
     3121 cgaccactgg ccacgtgccg gataacccgc tggataccac gatacatggc ggcaatctcg
     3181 ccgccgcggg cgcgatgccg aaggcgccgt cgaccgccgg aacaccgccc gcaaacccgc
     3241 tttcgagtag ttctggagcg aatggcgcga ccgagactgg cgccactgca tccgcttcgc
     3301 tgcagcgggc gcgggaactt gatcaggtcg gcgacgaagc tggttgcatg aacgagatca
     3361 ccaaggcaaa gcaactactt ggaactgatt aggacagcgt caaggggcga aacgaaagaa
     3421 gggtcctgtg gggccgaggc tggaacaggt ggggtggatt gacgattgag agaggccggc
     3481 tgttagtccg taactgccat tgacttgcct gactccgcgg cgttgtcggg ccagtgaaaa
     3541 aggctgtggg gaatgttaag aataacggac ctgaattgct ggaggcagcg caatgaacga
     3601 tcagggccgc tccctcgaac gtcgcaggct attgcgttgg ctgggggccg ccgcgctgtt
     3661 ccttccggct gcgctcgcag cgtgtgcaaa ctccgatgca ccgagacagt atgagcgaac
     3721 cccgagcgac attaggggaa gtgaccatcg cgacgggcaa tctgatcgca tcaatcttat
     3781 cgggcgtacg tataggtttt gatagcaccc gtcaccgcgc cgcaaacaaa agcccgccac
     3841 ctgcggcggc tgccggtgac gtggtacgtc gattggcact ctccaggggc tgatatgatg
     3901 gcatgacttt ccgctagtcc gacctggacc gcatcaatcg tataatcgag gaaagcaatc
     3961 ggcgcatcgt ggcgcacgaa gaccgcactg agcggctccg gcgatttcgc cattaggtcc
     4021 gctatgggcc acgaatcgac actcccgtga gctcctccat agcgacccct cacacaacgg
     4081 gcagggatcg gtacgcgcgc taatagggat acgggtagta gtaatagcca gggtagtagt
     4141 agccgggata agccggatac atgcgatagg catagagcgg cgggtaggaa gcagagccgt
     4201 tgtgcgccat cggcacgctc tcgcccttgg ccgacatgca ctgggtatag gccatgtcgt
     4261 agcggctctg gagagcggcg ccggaggcat acgcgttcga cacaccggca gagctcccca
     4321 ccagcaggcc gctgccgccg ccgatggcag caccgatcgc gggatcgccg gcggcg
//
LOCUS       DASSRL010000285         6454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_969865, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000285 DASSRL010000000
VERSION     DASSRL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969865"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFL49_05895"
     CDS             <1..641
                     /locus_tag="VFL49_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7911541.1"
                     /translation="AILRLISSDGPIVFMGNPIQELRFKEMRPYRHNMQIVFQDPYGS
                     LSPRMSVSDIIEEGLWVHHPGLSAAERERRVVASLNDVGLDSQTRYRYPHEFSGGQRQ
                     RIALARALVLEPTFIVLDEPTSALDMLIQSQMVDLLRDLQKRHDLTYMFISHDLRVVA
                     ALASRLLVMRHGKAVEEGAAAELFKAPKSDYTRALFAAAFKLETAPEGVVSQ"
     gene            641..1321
                     /locus_tag="VFL49_05900"
     CDS             641..1321
                     /locus_tag="VFL49_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7911542.1"
                     /translation="MRTIVIWLAAFVCVVATAAGAAQFGPDQYPIKSDEGDPITNFDL
                     TPELSARLVKLPGQIAVGNQNGDVTLVQFYDLNCPFCREAAADVDALVRADKKLKLVF
                     APYAVLSVQSAQGALVELAAGEMLTPEKYLEFHRRIYAGRGLIDAPRVLAAAEAIGLD
                     RQELAESANTESMLAVLKQNADFGSDAKLIATPAYVINGVAILGHPGFKSLQGVIRSV
                     RTCRKVTC"
     gene            complement(1318..2157)
                     /locus_tag="VFL49_05905"
     CDS             complement(1318..2157)
                     /locus_tag="VFL49_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7911543.1"
                     /translation="MVALDPRINPFRPEIAASHLRGQVEAKRFVEGSRHQIIEPATAL
                     RRVPSHDARLDTEALLGERVTIYERTDEGWAWGQLETDGYVGWLSSNALGAVGAAPTH
                     KIVALRAFAFAGPDIKLPPAAALPMGARLAVTRQDERFAVTALGWHIPEVYLAPLKAR
                     QADFVTVAERFVGAPYLWGGKTSLGLDCSGLVQVSLQAAGISCPRDSDMQELALGKLS
                     SLASLRRGDLVFWKGHVAIAIDPETLIHANAHHMMVAVEPAVDAITRIRAAGSEITSV
                     RRV"
     gene            complement(2161..3537)
                     /locus_tag="VFL49_05910"
     CDS             complement(2161..3537)
                     /locus_tag="VFL49_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HET7911544.1"
                     /translation="MHPIFANPGRNQTIPVWFVHAANFDAIRKRLGESTQTFIATAGF
                     EARPGRSLLMPGSKGELAGVLFGIESPDDADLDRFRPGQLAGLLPSGTYRFANAPHDA
                     RLAALAIALSAYQFSRYRKSETRNVRFVLPDGVDGEELSRVVEGVTFCRDLTNTPSND
                     MGPAELEAAARALAKQHSAKMQVTSGDALAKNFPLVHAVGSGSPRSPRLMDMTWGKGS
                     DPKITLVGKGVCFDTGGLDIKPDTGMLNMKKDMGGAATALALAHMVMAAGLKVRLRVI
                     IPAVENAISGTSFRPRDVYMSRKGISVEIGHTDAEGRLILADALALADEDKPSLVIDF
                     ATLTGAARVALGPDVPPFFTDDDALADELMRAAAAENDPLWRLPLWRPYGSLLDSKVA
                     DLNNVGTGGQAGAITAALFLRRFVSTKAWVHFDIFAWTPAAKAGRPEGAECQSARALY
                     ALLRARYS"
     gene            complement(3540..4769)
                     /gene="acdA"
                     /locus_tag="VFL49_05915"
     CDS             complement(3540..4769)
                     /gene="acdA"
                     /locus_tag="VFL49_05915"
                     /EC_number="3.13.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-sulfinopropanoyl-CoA desulfinase"
                     /protein_id="HET7911545.1"
                     /translation="MDMDLGLSADEMALRDRARTFAAEVVRPRAAAIDRDEMYPWDIV
                     KALGQSGFCGMTIPKALGGQGRSFLDAVVVIEEMAKACTVTSRIVVETNMGAISTVMA
                     YGTESQKRLAAELVLQGDKPAICITEPEAGSDAMAMTTRAVRCGDTYIINGKKHWITG
                     AGISRLHLIFARVFDERGEELGIGGFLAVHGQSKGLRVGKREQTMGLRGMPEGEIICE
                     DLELPASMALLPPSGFRRGFGDLMNAYNSQRVGAGTVALGVAAGALDHALNWVKTREQ
                     FGRPIGEFQGLQWMLAEMQIQLTAARLMLYQAARSRGPENSAFPDPMLAAQAKVFASE
                     TAIRIVNDALQFFGARGYSRELPLERMARDVRMFTIGGGTAQVLRTLVASKMLGWKLP
                     QRRDGYSPKSSMRDAAE"
     gene            4848..5660
                     /locus_tag="VFL49_05920"
     CDS             4848..5660
                     /locus_tag="VFL49_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7911546.1"
                     /translation="MRSRTGPSHLLASAAVLAILALSAGACSNIGQREVTGSVSGANT
                     ASLTQEEARRSLETLGARYRQNPNDADAAITYAKALRLTDQRAQAVAVLEQASIRNPN
                     SMPLLGAYGRALADAGQYQQALTTLERAHTPDNPDWRVLNVQGAVLDQMGRHAEAQKH
                     YASALKIMPNEPSVLSNLGLSYMLTKDLKNAEATLKRAAAQPDAGPKVRQNLALVVGL
                     QGRFDEAEKIASADLPDSEAAANVAYLRQMLAQKGDLKKMGRAYAPVADAGS"
     gene            complement(5692..6255)
                     /locus_tag="VFL49_05925"
     CDS             complement(5692..6255)
                     /locus_tag="VFL49_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET7911547.1"
                     /translation="MTAVTLRITKLLQSFRRDREGASAVEFALLAPLMIALYFGCVEV
                     TNGVAADRKVTLTAGALANLTSQSQTITLDGMTNILNASAAIIKPYSVGNLAATITCL
                     KIDADGNAKVKWSATLNGTARADGASVTLPSSALAVPNSSLVWSEVSYNYTPVIGYTI
                     TGTLSLSDQMFMSPRITPPVYGVKTCS"
     gene            complement(6266..>6454)
                     /locus_tag="VFL49_05930"
     CDS             complement(6266..>6454)
                     /locus_tag="VFL49_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HET7911548.1"
                     /translation="LNYSPGNPGQIEVVTLYYQWPITVSLLGNNLANINNNKRLLVAT
                     AVFCNEPFSTATPANSCH"
BASE COUNT         1238 a         1967 c         1991 g         1258 t
ORIGIN      
        1 tcgccattct gcgtctgatt tcgtcagatg gcccgatcgt gttcatggga aatccgattc
       61 aggagctcag attcaaggag atgcggccgt accgccacaa catgcagatc gtatttcagg
      121 atccgtacgg ctcgttgtcg ccgcgcatgt ccgtgtccga catcatcgaa gaggggctct
      181 gggtgcatca tccggggctg tcggcggcgg aacgcgagcg gcgcgttgtc gcgtcgttga
      241 acgacgtcgg tctcgattcg cagacgcggt accgctatcc gcacgagttc tccggcggcc
      301 agcgtcagcg tatcgcactg gcgcgtgcgc tggtgctgga gccgacattc atcgttctcg
      361 atgagccgac cagcgccctc gacatgctta tccagtcaca gatggtggac ctgctgcgcg
      421 acctgcaaaa gcgccacgac ctcacctaca tgttcatttc gcacgacctg cgcgtcgttg
      481 ccgcattggc gagccggctt ttggtcatgc gtcacggcaa ggcggtggag gagggcgctg
      541 cggccgaatt gttcaaggcg cccaagagcg attacacgcg ggcgctgttc gctgccgcgt
      601 tcaagctgga aaccgctcct gaaggcgtgg tgtcgcaata atgcgcacga tagtgatctg
      661 gctggcggcg tttgtctgcg tcgtggcgac ggctgccggc gcggcgcaat ttggtcccga
      721 tcaatatccc atcaagagcg acgaaggcga tcccatcacc aacttcgatc tgacaccgga
      781 gctgtcggcg cgactcgtaa agctgccggg ccagatcgca gtcggaaatc aaaacggtga
      841 tgtgactctt gtgcaattct acgatctcaa ctgtccgttc tgccgggagg cggctgcgga
      901 tgttgacgcg cttgtgcgtg ccgacaagaa gctcaagctg gtgttcgcgc cctatgcggt
      961 tttgtccgtg cagtcagctc aaggggccct cgtcgaactg gcggcgggcg aaatgctaac
     1021 tcctgaaaag tacctggaat ttcatcgccg catttatgcg ggccgtggcc tcatcgatgc
     1081 tccgcgcgtg ctcgcggcgg ctgaagccat aggtctcgat cgccaggagc tggccgagag
     1141 cgccaacacc gaaagcatgc tagccgtgct caagcaaaac gccgatttcg gctcagacgc
     1201 caagctcatt gcaacgcctg cttatgtcat caacggtgtg gccattctcg gccatcccgg
     1261 tttcaagtca ttgcagggcg tgatccgctc ggtgcgcacc tgccgcaagg tgacctgcta
     1321 aaccctgcga acactggtga tttcggagcc ggctgccctg atgcgcgtga tggcgtcgac
     1381 ggctggttca accgcgacca tcatgtgatg ggcatttgcg tgaatgagag tttcggggtc
     1441 aatcgcaatg gcgacgtggc ccttccagaa gacgagatca ccccggcgca ggcttgcaag
     1501 cgaagacagt tttcccaagg ccaattcctg catgtcgctg tcgcgggggc atgagatgcc
     1561 ggcagcctgc aacgaaacct ggacgaggcc ggaacaatcc agcccgagcg aggtcttgcc
     1621 gccccataaa tagggcgcgc ccacaaaccg ctccgcaacg gtgacgaaat cagcctgccg
     1681 cgccttgagc ggcgcaagat acacctcggg tatatgccag ccgagagccg tgacggcgaa
     1741 gcgctcgtcc tgccgggtga ccgcaagacg cgcgcccatc ggtaaagccg cggcgggtgg
     1801 cagcttgata tccggcccag caaaggcaaa tgcacgtagc gcgacgattt tgtgggtcgg
     1861 cgctgcaccg acggctccga gcgcgttgga cgaaagccag ccgacatagc cgtcggtttc
     1921 cagctggccc catgcccagc cttcatcggt tctttcgtag atcgtcaccc gttcacccaa
     1981 cagggcttcc gtatcgagcc ttgcgtcgtg cgaaggcacg cgccggaggg cggtggccgg
     2041 ttcgatgatt tgatgccggc tgccttcaac aaatcgcttg gcttcgactt gtccgcgcag
     2101 gtggcttgcg gcgatctcag gccggaaggg attgatgcgg ggatcgagcg cgaccatcga
     2161 tcaagaatat ctggcgcgga gtaatgcata gagggcgcgc gccgactggc attccgcacc
     2221 ttccgggcgg ccggcttttg ccgccggcgt ccaggcgaag atatcaaaat gtacccacgc
     2281 tttcgtgctc acaaaccggc gcaggaacaa agcagccgtg attgcgccgg cctgtccccc
     2341 ggttccgacg ttgttgagat cggccacttt cgagtcgagc aggctgccgt agggccgcca
     2401 aagcggcaaa cgccacagcg ggtcattctc ggctgcggcc gcgcgcatca actcgtcggc
     2461 aagggcgtcg tcgtcggtga agaatggcgg cacatcggga ccaagggcaa cgcgcgccgc
     2521 acccgtgagg gtcgcaaaat cgatcaccag cgacggcttg tcctcgtcgg caagcgcgag
     2581 ggcatccgcc aggatcaacc ggccttcggc gtcggtgtga ccgatttcga cggaaatccc
     2641 cttgcgcgac atgtaaacgt cacgcggacg gaagctcgtt ccggatatcg cgttctcaac
     2701 ggcaggaatg atcacgcgca aacggacttt cagtccggcc gccatgacca tgtgcgcaag
     2761 tgcaagcgca gtcgccgcgc cgcccatatc cttcttcatg ttgagcatgc cggtgtcggg
     2821 cttgatatcg agcccgccgg tatcaaaaca gacgccttta ccgaccagcg tgatcttcgg
     2881 atcgctcccc ttaccccacg tcatgtccat cagccgcggc gagcgaggcg atccggaccc
     2941 aaccgcgtgc acaagcggaa aattcttggc cagcgcatcg ccgctcgtca cttgcatttt
     3001 cgcggagtgc tgcttggcga gcgcccgggc cgcagcctca agttcggcgg gacccatgtc
     3061 gtttgacggc gtatttgtca ggtcacggca gaacgtcacg ccctcgacga cgcggctcag
     3121 ctcttcgccg tcgacgccgt ccggcagcac gaagcgaacg ttgcgagttt ccgattttcg
     3181 atatcgcgag aattgataag cgctgagcgc aattgccagt gcagcaagcc gtgcgtcgtg
     3241 cggcgcattg gcgaaacgat atgttccgga aggcaaaaga ccggcgagtt ggcccggccg
     3301 gaaccggtca aggtccgcgt cgtccgggct ctcaatcccg aacaggacgc cggcaagttc
     3361 gcctttgctg cctggcatca gcagcgaccg gccgggcctc gcctcgaagc cggcagtggc
     3421 gatgaacgtc tgtgtggatt cgccgagcct ctttcgtatt gcgtcgaaat tggcggcatg
     3481 aacgaaccag acgggaatcg tctggttcct gccggggttc gcaaatatcg gatgcatgcc
     3541 tactccgcgg cgtcgcgcat cgaggacttg ggagaatagc cgtcacgcct ttgcggcagt
     3601 ttccagccca gcatcttcga agcgaccagc gtgcggagta cctgtgccgt cccgccgccg
     3661 atcgtgaaca tccgcacgtc ccgtgccatg cgctccagcg gcaattcgcg cgaataacct
     3721 cgcgcgccaa aaaactgcag ggcgtcgttg acgatcctga tagcggtttc ggatgcaaat
     3781 accttggcct gcgccgcgag catcgggtcg gggaacgcgc tgttctcggg gccgcgcgag
     3841 cgcgcggcct gatagagcat gagccgggcc gccgtcagct ggatctgcat ctcggcaagc
     3901 atccactgca gaccctgaaa ttcgccaata ggccggccga actgctcgcg ggtcttcacc
     3961 caattcaaag catggtcgag tgcgccggca gcgacgccga gtgcaacggt acccgcgccg
     4021 acccgctgac tattgtaggc attcatcagg tcgccaaagc cacggcgaaa ccccgacggc
     4081 ggcagcaagg ccatgcttgc cggcagctca aggtcctcgc agatgatttc gccctcgggc
     4141 attcctcgca gtcccatggt ctgctcgcgc ttgccaaccc tcaggccttt cgattgcccg
     4201 tgtactgcga gaaagccgcc aattccaagc tcctcgccgc gctcgtcaaa cacgcgcgca
     4261 aaaatcaggt gcagacgtga gatgcccgcg ccggtgatcc aatgcttctt gccatttatg
     4321 atgtacgtat caccgcacct gaccgcgcgg gtggtcattg ccatcgcatc ggatccggcc
     4381 tcgggctcgg tgatgcagat cgccggcttg tcgccttgaa gcacgagttc agccgccagc
     4441 cttttctgtg attccgtgcc gtacgccatc acggtcgaaa tcgcgcccat gttggtttcc
     4501 acaacgatgc gcgaggtgac cgtgcaagcc ttcgccatct cttcgatcac cacgaccgca
     4561 tcaaggaaag agcggccctg gccgccgaga gccttcggga tcgtcatgcc gcagaagccc
     4621 gactgaccca gcgctttgac tatgtcccac gggtacattt cgtcgcggtc aatggccgcg
     4681 gcgcgcgggc gtacgacctc ggcggcgaaa gtgcgggcgc gatcgcgcag tgccatttcg
     4741 tcggcggaga ggcccaggtc catgtccatg acggcgacca taaacgaagt gtggcggtga
     4801 gtttacaagt taaccagtca ttagggttaa cgttttattg ctcgtccatg cgatcgagaa
     4861 ccggccccag tcatctgctc gcctcagctg ccgtgttggc catcctggct ctgtcggcgg
     4921 gtgcttgctc caatatcggt cagcgcgagg tgacaggctc ggtaagtggc gcgaacactg
     4981 cgtcgcttac ccaggaggaa gcccgacgct cgcttgagac gcttggcgcc cgctatcgcc
     5041 agaatccgaa cgatgcggat gcggcgatca cctacgcaaa ggcattgcga ttgaccgacc
     5101 agcgcgccca ggctgtggcg gtgctggagc aggcttcgat ccgtaatcca aacagcatgc
     5161 ctttgctcgg tgcctatggt cgcgcgttgg cggatgccgg ccagtaccaa caggcgctga
     5221 cgacgctcga gcgcgcacac accccggaca atccggattg gcgcgttctc aacgtgcaag
     5281 gggccgtgct tgatcagatg ggtcgccatg ccgaagcgca aaaacattat gcctccgcgc
     5341 tcaagatcat gccgaacgaa ccatcggtgc tgtccaacct tggcctgtcg tatatgctca
     5401 cgaaggatct gaaaaacgcc gaggcgactt tgaagcgcgc cgccgctcag ccggacgccg
     5461 ggccgaaggt ccggcagaat ctggcgcttg tcgtcggtct gcaaggccgt tttgatgaag
     5521 cagagaagat tgcgagcgcc gacctgccgg acagcgaagc ggcggcgaac gtcgcttatc
     5581 tgcggcagat gctcgcgcaa aagggtgatt tgaagaagat ggggcgtgct tacgcgccgg
     5641 tcgccgacgc gggatcctga gctgcttttc tgacgaacgg gacgtttagc gtcagctgca
     5701 agtcttgacg ccgtagacgg gcggcgtgat gcgcgggctc atgaacatct ggtccgatag
     5761 cgacagcgtg cccgtaatcg tataaccaat caccggagta taattgtagc tcacttcgct
     5821 ccacaccagc gaagaattcg gaaccgccag tgccgacgac ggaagggtca cggaggctcc
     5881 gtcggcgcgt gccgtgccgt tcaaggtagc gctccatttg accttggcgt tgccatccgc
     5941 gtcgatcttc aggcatgtga tcgtcgctgc cagattgccg accgaatacg gtttgatgat
     6001 cgccgcagag gcattcagaa tattggtcat gccgtcgagc gtaatcgttt gggattgaga
     6061 cgtcaggttg gcgagcgcgc cggctgtgag cgtcaccttg cgatccgccg caacaccgtt
     6121 ggttacttcc acgcatccga aatagagcgc gatcatgagt ggtgcgagga gggcaaactc
     6181 gaccgccgag gctccttccc ggtcacgcct gaatgattgc agcagcttgg ttatacgcag
     6241 tgtcacggct gtcatgacgg atcgcctaat ggcaggaatt tgccggggtg gcggttgaga
     6301 atggttcgtt gcagaagact gccgtggcca ccaggagacg tttgttgttg tttatgtttg
     6361 cgaggttgtt tccgagcagc gaaaccgtga tcggccactg atagtaaagt gtcacgactt
     6421 caatctggcc tgggtttccc gggctatagt tcag
//
LOCUS       DASSRL010000286         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1565089, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000286 DASSRL010000000
VERSION     DASSRL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565089"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFL49_05935"
     CDS             <1..288
                     /locus_tag="VFL49_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET7911549.1"
                     /translation="RLAGWTILSHLLQVLTWGVVYATAGAIRGLASAFYFSAVTYTTT
                     GYGDLVLPQQWRVVGGIEALTGILMCGLSTGLFFGVFARVAGIIAPTAPAQ"
     gene            364..1803
                     /gene="pyk"
                     /locus_tag="VFL49_05940"
     CDS             364..1803
                     /gene="pyk"
                     /locus_tag="VFL49_05940"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7911550.1"
                     /translation="MNPALSAHKTKIVATIGPASDSPEMLVRLIRAGVSVARLNFSHG
                     DFSQHGETIRRIREAARETGRRVAILADLPGPKMRLGKIDPEPIQLTPGARFTLTNEE
                     IVGNAERVSMSFEPLPRVVRPGNRLFLNDGLVQLVVDRVSGNDVECKVAVGGELRSRK
                     GLNLPGIHLGIGAFTEHDRACLEFALHARVDAVSQSFVERAADLEAVRAAATKIGRQP
                     FLIAKIERSDALAHIDEIVAAADGIMVARGDLGVEVPIEEIAYTQKQLIAKANLAGKP
                     VITATQMLESMVTSRLPTRAEATDVANAIIDGTDCVMLSGESAMGKFPEEAVLMLAKI
                     AAFTEAHRPRTTLAARREVLEQEAEKMSGGDRMTSVVEHALDTVPCDLVCVPTRSGTT
                     ARRISRFKPGVWLIAPSEDPAVCQGLAFSYGVHAVDLTEEPDDDWRTFVEGWVRAHGI
                     PASRVMLVAGPSARNPHANHRVELMKLRA"
     gene            complement(1808..>2216)
                     /locus_tag="VFL49_05945"
     CDS             complement(1808..>2216)
                     /locus_tag="VFL49_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003589375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HET7911551.1"
                     /translation="VEAVHDAAMAFGMTPAFVGFIVVALVGGAAEMVAAFSAARRNQL
                     DLSISIALGSAAQIALFVAPVLVLLSRVIGPAPMNLDFWPGAVAMVFLATLTAALVTN
                     SGRSAWFNGVLVMMVYLMFAAALYLLPPAISTP"
BASE COUNT          433 a          701 c          688 g          394 t
ORIGIN      
        1 cgcctggccg gctggaccat cctttcccat ttgctgcaag tcctaacctg gggcgtggtc
       61 tatgcgacgg ccggggcgat ccgcggtctt gccagcgcct tttatttcag cgcggtgacc
      121 tacacgacta cgggctacgg cgatctggtc ctgccccagc aatggcgcgt cgtcggcggc
      181 atcgaagcgc tcaccggcat cctcatgtgc ggcttgagca caggactttt ctttggcgtt
      241 ttcgcccgcg tcgccggaat catcgcacca accgcaccgg ctcaatgagt catggtccaa
      301 gcatcagaga ctggcagcgt cgattgggcg tcgccatgct tgtggtaaaa caacatcccc
      361 gtcatgaatc cagctctttc cgcacataaa accaagatcg ttgccacgat cggtccggcc
      421 tcggattcgc cggagatgct ggtgcgactg attcgtgccg gcgtcagtgt cgcgcgcctc
      481 aatttctccc acggcgattt ttctcaacac ggagaaacca ttcgccggat ccgcgaagcg
      541 gcacgcgaaa ccggccggcg cgtagcgatc ttggccgacc tgccaggtcc gaagatgcgg
      601 ctgggcaaga tcgatccgga gccgatccag ctcaccccgg gtgcgcgctt caccctgacg
      661 aatgaagaga ttgtcgggaa tgcggagcgc gtctccatga gtttcgagcc gctgccgcgg
      721 gtcgtcaggc cgggcaaccg tctctttctc aacgacggcc ttgttcagct cgtggtcgat
      781 cgcgtttccg gcaacgacgt cgagtgcaag gtcgcggttg gcggcgaact gcgctcccgc
      841 aaaggactta acctcccggg aatccacctc ggaattggcg ctttcaccga gcacgaccgc
      901 gcctgtctcg agtttgcgct ccacgccagg gtcgatgcgg tgagtcagtc gttcgtcgaa
      961 cgcgctgccg atctcgaggc ggtccgggcc gcggcaacga aaatcggacg gcaaccgttc
     1021 ctgatcgcaa agattgagcg atcggacgcg ctcgcacaca tcgatgaaat tgtggcggcg
     1081 gctgacggca tcatggtggc gcgcggcgac ctcggagtcg aggtcccaat cgaagagatc
     1141 gcatacacgc aaaagcagtt gatcgcgaaa gccaacctgg cgggcaagcc ggtcatcacc
     1201 gcaacccaga tgctcgagtc gatggtaacg agccgtttgc ccacccgggc cgaagcgacc
     1261 gacgtggcca acgccatcat cgacggcacc gactgcgtca tgctctcggg tgaatccgcc
     1321 atgggtaaat ttccggagga agcagtgctg atgctggcca agatcgctgc gtttaccgag
     1381 gcgcaccggc cacgcaccac cttggcggca cggcgggaag tgttagagca agaggcggag
     1441 aaaatgagcg gcggcgatcg catgacctcg gtggtcgagc atgctctcga taccgtcccg
     1501 tgcgatctcg tctgtgttcc gacccggagc gggaccaccg cccgaaggat ttcgcgcttc
     1561 aagccgggag tctggctgat cgctccgagt gaggatccgg cagtttgtca gggtcttgcc
     1621 ttctcttacg gcgtgcacgc cgtcgatctg acggaagaac cggatgacga ttggcggacg
     1681 ttcgtcgagg gttgggtccg agcgcacggc attcccgcca gccgcgtcat gctggtagcc
     1741 ggtccctccg cccgtaaccc ccacgccaat catcgcgtcg agttgatgaa gctgcgcgcc
     1801 tgacgattca gggcgtcgaa attgccggtg gcaagagata gagggcagcg gcgaacatca
     1861 ggtaaaccat cataaccagc acgccattga accaggcgga ccggcccgag ttggttacca
     1921 gcgccgcggt cagcgtagcc agaaaaacca tcgcgaccgc ccccggccag aaatccaggt
     1981 tcatgggcgc ggggccaatc acacggctaa gcaggaccag caccggcgcg acaaacagag
     2041 caatctgggc cgcgcttccg agcgcaatgc tgatactcag gtcgagttga ttacgacgtg
     2101 ccgcggagaa cgccgccacc atctcggctg ctccgcccac cagggcgacg acgatgaagc
     2161 caacgaaggc gggggtcatt ccgaacgcca tcgcggcgtc gtggaccgct tcgacg
//
LOCUS       DASSRL010000287         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1565102, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000287 DASSRL010000000
VERSION     DASSRL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565102"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="VFL49_05950"
     CDS             complement(<1..1793)
                     /locus_tag="VFL49_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911552.1"
                     /translation="MNLGVSFAPLVPSYVVWAAFAAAALISVILLIARSRGAPLRALA
                     LALMVLALANPSLTREDREPIPSVAAVIVDKSPSQSFGDRTQQTEAARSALVERLKRI
                     PGLEVRVAEAGAADGETDGTKLFAALSSTLADVPPDRVAGAILITDGRVHDVPADASA
                     LGFSAPLHALITGHKDEIDRRVVLTQTPRFGIVGQSQTVGFRVEDQGAKVGAAEVTVR
                     RDGEVLSKRVVTVHTDVKFNVPIPHAGPNIVEIEASPLDGELTPVNNRAVVSIDGVRD
                     KLRVLLVSGEPHAGERTWRNLLKSDASVDLVHFTILRPPEKQDGTPINELSLIAFPTR
                     ELFQQKISEFQLIIFDRYARQGVLPLIYFDNIAKYVRDGGAVLIAAGPDYASPTSIWR
                     TPLDGILPAEPTGEVTERRFQPQLTEAGRRHPVTRDLEGSDSDPPHWSNWFRLVDIKR
                     STGTNVMQGPDGKPLLVLGREGQGRVALLLSDHIWLWARGYEGGGPHLDLLRRLSHWL
                     MKQPDLEEEALRLIVRGRDITVQRQTMADTVNQITLTSPTGATRTIDLQAVEPGLWRA
                     ATTANELGLWRATDGKLTALANVGPANPREFA"
     gene            complement(1803..>2951)
                     /locus_tag="VFL49_05955"
     CDS             complement(1803..>2951)
                     /locus_tag="VFL49_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7911553.1"
                     /translation="PSNYTQRGSLDHPPGFYGPPEGLVAVNTLAPADRPALLDTSSLN
                     ARVDAYRQGEPLDLRGPIFLAALALLVLDALVVIWLSGGLSAMRPRRRATVAAIALAL
                     ATGLVLQHNPAQAQSAPAQPQPLTAQQLDFAMKATLQTHLAYVVTGDAAVDEVSKNGL
                     QGLTMFLAQRTALEAGDPIGLDPARDELAFFPLIYWPIAPGAPKPTQAALDKIDAYMK
                     RGGTVLFDTRDAVDAPAGRGGEMRGPGMVALRSILSSLDIPELEPVPHDHVLTKTFFL
                     LRDFPGRFTNGQLWVEALPAAGDEEENNRPARAGDGVSSILITSNDLAGAWALRPDGQ
                     PMLPLVPGEPRQRELAFRAGVNIVMYALTGNYKADQVHIPALLERLGQ"
BASE COUNT          513 a          937 c          993 g          508 t
ORIGIN      
        1 gcaaattcac gcgggttggc gggtccgaca ttggcaagcg cggtgagctt gccgtcggtt
       61 gcgcgccaga ggccaagctc gttcgcggtc gttgccgccc gccaaagtcc gggctcgacc
      121 gcctgaagat cgatcgtgcg cgttgcgccg gtgggtgacg tcagcgttat ctgattgacg
      181 gtatcggcca tcgtctgccg ctgcacggta atgtcgcggc cgcgcacgat gagccgcaac
      241 gcctcttcct cgaggtcggg ctgcttcatg agccagtgcg acagccgccg cagcagatcg
      301 agatgcggcc cgcccccttc gtagccgcgc gcccaaagcc agatgtgatc tgacagcagg
      361 agcgccacgc gaccctggcc ttcgcgtccc agcaccagga gcggcttgcc gtccggtcct
      421 tgcatcacgt tcgtgccggt gctgcgcttg atgtcgacca gccggaacca gttgctccag
      481 tgtggcgggt cgctgtccga tccttcaaga tcacgcgtca ccggatgccg ccggcccgct
      541 tcggtgagtt ggggctggaa tcgacgctca gtcacttcgc cggtgggctc ggccggaagt
      601 atgccgtcga gcggcgtacg ccagatgctg gtcgggctcg cataatcagg tccggccgcg
      661 atcagaaccg cgccgccgtc gcgcacgtat ttcgcaatgt tgtcgaagta gatcagcggc
      721 agcacgccct gcctggcata gcggtcgaag atgatgagct gaaattcgct gatcttctgc
      781 tgaaacagct cgcgggtcgg aaacgcaatc aacgacagct cgttgatcgg cgtgccgtcc
      841 tgcttttccg gcggccgcaa aatggtgaag tggaccagat cgacggaggc gtccgacttg
      901 agcagattgc gccacgtgcg ctcaccagca tgtggctcgc ccgaaaccag caatacgcgc
      961 aacttgtcac gcacgccgtc gatcgagacg accgcgcgat tattgaccgg cgtcagctcg
     1021 ccatcgagcg gcgatgcttc gatctcgacg atgttcggtc ccgcgtgcgg gatcgggaca
     1081 ttgaacttga cgtcggtgtg cacagtgacg acacgcttgc taagcacctc gccgtcgcgg
     1141 cgcacggtga cttcggccgc gccgaccttc gcaccttggt cttcgacgcg gaatccgacc
     1201 gtctgggatt gcccgactat tccgaaacgg ggcgtctgcg taagcacgac acggcgatcg
     1261 atttcgtcct tgtggccggt gatcagcgca tgcaacgggg ctgagaaccc gagcgcggat
     1321 gcgtctgccg ggacatcatg caccctgccg tcggtgatca gaatcgcgcc ggcgacgcgg
     1381 tcaggcggaa catctgcgag tgtggacgaa agcgccgcaa acagcttggt gccatcggtt
     1441 tctccgtcag ccgcgccggc ctccgcgacg cgcacttcaa ggcccgggat gcgtttgagc
     1501 cgctcgacca gcgcactacg ggcagcttcg gtctgctgcg tgcgatcccc gaaactctgg
     1561 ctcgggctct tgtcgacgat caccgcggcc accgatggaa tgggctcgcg gtcttcgcgc
     1621 gtgagcgaag gattggcgag cgccagcacc atcagcgcca gcgccagcgc gcgaagtggc
     1681 gcgccgcggc tccgcgcaat cagcaagata acggagatca gcgcagcagc agcaaatgct
     1741 gcccacacga cgtaggaagg gaccagcgga gcaaatgata cgccgaggtt cacgatacgc
     1801 tcctactgcc ccagccgctc gagtaaagcg gggatgtgca cctgatcggc cttgtagttg
     1861 ccggtcagcg cgtacatcac gatgttgacg ccggcgcgga atgcgagctc gcgctgacgc
     1921 ggctcgcccg gcaccagcgg cagcatcggc tgtccgtcgg gccgcagcgc ccacgcgccg
     1981 gcgaggtcat ttgacgtgat caggatcgag gagacgccgt cgccggcgcg ggccggacga
     2041 ttgttctctt cttcgtcgcc ggcagcgggc aatgcttcga cccataattg cccgttggtg
     2101 aagcggcccg gaaaatcgcg cagaaggaag aatgtcttgg tcagcacgtg atcgtgcggc
     2161 accggctcaa gctcgggaat gtcgagagaa gacaggattg atcgcaatgc gaccatgccg
     2221 ggcccgcgca tttcgccgcc gcggccggcc ggcgcgtcga cggcgtcgcg ggtgtcgaac
     2281 agcactgtgc cgccgcgctt catgtaggcg tcgatcttgt cgagcgcagc ctgcgtcggc
     2341 ttcggcgccc ccggcgcaat cggccagtag atcagcggga agaatgcgag ctcgtcgcgc
     2401 gccgggtcga gcccgatggg gtcgccggcc tcaagcgccg tgcgctgggc gaggaacatt
     2461 gtgaggcctt gcaatccgtt cttgcttact tcgtcgacgg cagcgtcacc cgtgacgacg
     2521 taggcgaggt gcgtctgcag cgtcgccttc atggcgaagt ccagctgctg cgccgtcaac
     2581 ggttgcggtt gcgcgggcgc cgactgtgcc tgagcaggat tatgctgaag caccaggccg
     2641 gtggccaatg cgagcgcaat cgccgcgacg gtggcgcgac ggcgtggccg catcgcagag
     2701 aggccacccg aaagccagat caccaccagc gcgtcgagca cgagcaatgc caatgcggcg
     2761 aggaatatcg gtccacgcag gtcgaggggc tcgccttgcc ggtacgcatc gacacgtgcg
     2821 ttaagcgacg atgtatcgag cagtgcgggg cggtcggcgg gcgcaagcgt attcaccgcg
     2881 accagccctt cgggcggccc gtaaaaaccg ggcgggtgat cgaggctgcc gcgctgggtg
     2941 tagttgctgg g
//
LOCUS       DASSRL010000288         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_497542, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000288 DASSRL010000000
VERSION     DASSRL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497542"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFL49_05960"
     CDS             <1..385
                     /locus_tag="VFL49_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911554.1"
                     /translation="LIHGLPQDVADQGIDTQHESLGSAFLSQHFGPAVSEPVRLHVAA
                     KRYLATTERGYYGLLSPASIQSLQLQGGLMSAEERTCFAAERFAGDALRLRRWDDEGK
                     IIGARTPDLLHFERYLDASLRSLSA"
     gene            599..1273
                     /locus_tag="VFL49_05965"
                     /pseudo
     CDS             599..1273
                     /locus_tag="VFL49_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
     gene            1598..2398
                     /locus_tag="VFL49_05970"
     CDS             1598..2398
                     /locus_tag="VFL49_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7911555.1"
                     /translation="MLIAVAAGSVLFHLLSPWWWTPIASNWNYIDHTITITFWITGFV
                     FTAIVVFMAYCVWRFHHRPGLQAAYEPESTKKEVWLTIGTTVGVAAMLAPGLIVWHQF
                     ITVPETASQIEIVGQQWQWNYRLPGKDGKLGAFEAQHITDQNPLGLNPNDPNSQDDVV
                     ITADDFHLPVGKPVKVLLRSIDVLHDFYVPEFRAKMDMIPGMVTYFWFTPTRTGTFDV
                     LCAELCGVGHAYMRGRVVVENEVDYQAWLGQHQTFAQSLQLPKKQAAN"
     gene            2434..>3178
                     /locus_tag="VFL49_05975"
     CDS             2434..>3178
                     /locus_tag="VFL49_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7911556.1"
                     /translation="MIDISRGATDLIPPAEVKDVDLYHPKSWITKYVFCQDAKVIAIQ
                     YSLTALAIGMLALVLSWLMRLQLGFPGVFSFIDAEHYYQFITMHGMIMVIYLITAIFL
                     GGFGNYLIPLMLGARDMVFPYLNMLSFWFYLLAVLVLVAGFFIPGGPTGAGWTLYPPQ
                     AILSGTAGREWGIILMLVSLMLFIIGFTMGGLNYVVTTLQARTRGMTLMRMPLTVWGI
                     FIATVMAMLAFPALFVACVMMLFDKLLGTS"
BASE COUNT          532 a          993 c          987 g          666 t
ORIGIN      
        1 tctgatccac ggcctgccgc aggatgtggc cgaccagggc atcgacacgc agcatgagag
       61 cctcggctcc gccttcctgt cgcagcattt cggcccggcg gtgagcgagc cggtgcgcct
      121 gcacgtcgcc gcgaagcgct atctcgcgac cacggagcga ggctactacg gcctgctgtc
      181 gccggcttcg atccagagcc tgcaattgca gggcggcctg atgtcggcgg aggagcgcac
      241 ctgcttcgcg gcggagcgct tcgccggaga cgccctccgg ctgcggcgtt gggacgacga
      301 aggcaagatc atcggcgcca ggacgcctga cctgttgcac ttcgaacgct atttggacgc
      361 cagcttgcgg tcgctatcgg cctgaaaatg cagggtatgt tcgcgccagg gtgagacaaa
      421 atggcgcaga cggccgtcag tgagcggagt atagttccct gatgactggt cgcggcgttg
      481 ccgtgaccgg tatcggactt gggtacgcgc caggcgcggt atccgtgctg gcgggaccat
      541 cgaagatccg gcccggcgtg ccggcgacct ctcgaactcg cggttcggag gtgcggcgat
      601 gccccagacg atccgaaacc cggttgagtg gctagcggac gaagtccgag aagtggccag
      661 agggatcggc gacgcgagcc gctccgtcca ccgaacctcg acgcatctgg tttcccgacg
      721 gctgacggtc aaccggattg cggtcgacga tctgaaggac gtactggtca agggcttcga
      781 tgacttcggt gccttccgga ccgacatcat cttcctgatc gtcatctatc ccatcatttc
      841 attcgtcctt ggcgcagcgg ccctccacca cgagctcatt ccgctgctgt tcccgctcgc
      901 ctccgggtcg gtcatcatgg ggccgttcgt gggcgtgttc ctgtatgaaa tgagccgtcg
      961 gcgcgagctg aatctcgagc gccaccacga agacaaccat agttgggcca acgcgctcag
     1021 cgtggtccgc tcgcgcaatt tcggcgcgat catactgctc ggcctgatcc tggccgcgat
     1081 gtacctgatc tggctcggcg cggcctgggg catctatcag gcgacgcatg ggccgcagcc
     1141 ggtcgaatcg gccggtcgct tcatccagga tctgttcctg accgaggccg gctggtggct
     1201 gatcggtctc ggttgcggcc ttggtttcct gtttgcggcg ctgacgctgg tggtcaccgt
     1261 cgtatcgttc ccgctgctgc tcgaccgcga ggtcgggctc atcaccgcgg tctcgacgtc
     1321 gatcggcgct gcagctgcca atcccatccc gatcgcggct tggggcttca ttgtgggcgc
     1381 ggggctggtg atcggtgcga tcccactttt catgggcctt gttgtcgtca tgccggtgct
     1441 gtgccacgca acctggcacc tctatcgcaa gctggttccg cggcaggcgc cggcgccccc
     1501 gccgcaccat catcgcaagg cggccgtgcc gccggcgtcg gctgtcccgg tcgacacgct
     1561 cgagcagagc cttgatgaag ggcagaccgt tgcgctcatc ctcatcgcgg tcgcagccgg
     1621 ctccgtattg ttccacctgc taagcccatg gtggtggaca ccgatcgcgt cgaactggaa
     1681 ctacatcgac cacacgatca ccatcacctt ctggatcacg ggtttcgtct tcacggcgat
     1741 cgtggtcttc atggcctatt gcgtctggcg ctttcatcac cgcccgggac tccaggcggc
     1801 gtatgagcct gaaagcacga agaaggaggt gtggctgaca atcggcacaa cggtgggggt
     1861 tgccgccatg ctggccccgg gcctgatcgt ctggcaccag ttcatcacgg ttcccgagac
     1921 cgcgtcgcag atcgaaatcg tcggccagca atggcaatgg aactaccggc tgccgggaaa
     1981 ggacggcaag ctcggcgcgt tcgaggcgca gcatatcact gatcaaaatc cattgggtct
     2041 caatccgaac gatcccaata gtcaggacga tgtcgtaatc acggcggacg actttcacct
     2101 gccggtcggc aagccagtga aagtgctgtt gcgctcgatc gatgtgctgc acgatttcta
     2161 cgtgccggaa tttcgcgcca agatggacat gattcccggc atggtgacct atttctggtt
     2221 cacgcccacc cggaccggga cgttcgacgt cctgtgcgcg gaactgtgcg gcgtcggcca
     2281 tgcctatatg cgaggccgag tcgtggtgga gaacgaggtc gactaccagg cgtggctggg
     2341 acagcaccag acctttgcgc aatcgctgca gctgccgaaa aagcaagcgg cgaactagac
     2401 ctggtctttt gtgagaagac gaggaggcat tcaatgatag acatctcacg tggcgcgact
     2461 gatctgatcc cgcctgccga ggtcaaggac gtcgatctct atcacccgaa aagctggata
     2521 acgaaatacg tcttttgcca ggatgcgaaa gtgatcgcga tccagtattc gctgaccgcc
     2581 ttggccattg gcatgcttgc gctcgtgctg tcgtggctga tgcgtctgca gctcggcttc
     2641 cccggtgtgt tctccttcat cgacgccgaa cattactacc agttcatcac catgcatggg
     2701 atgatcatgg tgatctatct gattaccgct attttcctcg gcggcttcgg caactatctc
     2761 atcccgctga tgctgggcgc gcgggacatg gtgttcccgt acttgaacat gctcagcttc
     2821 tggttttatc tgctcgcggt gctggtgctg gtggccggct tcttcatccc cggcggcccg
     2881 acgggcgcgg gctggacgtt atatccgccg caagccattc tgtccggcac cgccgggcgc
     2941 gagtggggca tcatcctcat gctggtctcg ctcatgctgt tcatcatcgg gttcaccatg
     3001 ggcggactaa actacgtggt gaccaccctg caagcgcgca cgcgcggcat gacgctgatg
     3061 cgcatgcccc tgaccgtctg gggcattttc atcgccaccg tcatggccat gctggccttc
     3121 ccggcgctgt tcgtcgcctg cgtcatgatg ctgttcgaca agctgctcgg caccagct
//
LOCUS       DASSRL010000289         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_24915, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000289 DASSRL010000000
VERSION     DASSRL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24915"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /gene="purB"
                     /locus_tag="VFL49_05980"
     CDS             <1..1241
                     /gene="purB"
                     /locus_tag="VFL49_05980"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7911557.1"
                     /translation="FEIEAHAADAMAKLGTIPGDAAKKIWEKGKSAEFDVARIDAIEA
                     EVKHDVIAFLTHLSEIVGPEARFVHSGMTSSDVLDTCLNVQLVRAADILIADVDTLLA
                     ALKRRAFEHKFTPTVGRSHGIHAEPTTFGLKLAYAYAEFARARDRLAAARKEVATCAI
                     SGAVGTFAQVDPRVEAHVAKAMGLSVEPVSTQIIPRDRHAMYFATLGVVASSIERLAT
                     EIRHLQRTEVLEAEEYFTEKQKGSSAMPHKRNPVLSENVTGLARMVRAYALPAMENVA
                     LWHERDISHSSVERMIGPDATVTLDFALARLAAIVDKLIVYPAAMQKNLDRLGGLIHS
                     QRVLIALTQKGIAREDAYRLVQRNAMKVWAGEGDFLSFLKADKDVRATLTDREIEANF
                     NLDHHFKHIGTIFERVFGSA"
     gene            1256..2035
                     /gene="murI"
                     /locus_tag="VFL49_05985"
     CDS             1256..2035
                     /gene="murI"
                     /locus_tag="VFL49_05985"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET7911558.1"
                     /translation="MPQAPTILLFDSGLGGLTVFQQVRSARPDANFVYVADDAGFPYG
                     NLEEDRLIERVIKVVGHVISDSNPDLVVVACNTASTLALGELRAHFRVPFVGTVPAIK
                     PACAQSATKRIAVLGTQATVAREYTHTLIREFGSGCDVHLVGSTQLAASAEAELAGTP
                     VADAVIAGEIARCFIDKDGRRTDTIVLACTHYPLLLERFRANAPWPVVWLDPAPAIAR
                     RVADLMRARPNGGMPIAPRMIFTSGCAPSPQLAASLARYGF"
     gene            complement(2032..2661)
                     /locus_tag="VFL49_05990"
     CDS             complement(2032..2661)
                     /locus_tag="VFL49_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7911559.1"
                     /translation="MIDLTLVPQGAIAAVLQVIMIDLVLAGDNAVVIGLAAAGLPPAQ
                     RSKAILVGIIAATVLRLAFAAIAVELLAITGLLLAGGILLLWVCWKMWRDLRGPGAED
                     TEAALMKREAHVPRKTFAQAAWQIVLADVSMSLDNVLAVAGAARDHPYALIFGLALSI
                     VLMGVAANYIARLLNRHRWIAYAGLAIILYVSLDMIWRGYWELEPRIFG"
BASE COUNT          671 a         1061 c          977 g          581 t
ORIGIN      
        1 ggtttgaaat cgaggcacac gcggctgacg ccatggcgaa gctgggcacc atccccgggg
       61 acgccgccaa aaagatctgg gaaaagggca agtcagccga attcgacgtc gcgcggattg
      121 atgccatcga ggccgaggtc aaacacgacg taatcgcatt ccttacgcac ctgtctgaaa
      181 tcgtcggccc ggaagcccgc ttcgttcatt cgggcatgac atcgtcggac gtgctcgaca
      241 cgtgcctgaa cgtacaactc gtgcgagctg ccgacatcct gatcgcagat gtcgatacat
      301 tgctcgccgc gctcaaacgt cgcgctttcg agcacaagtt cacgccaacc gtcggtcgct
      361 cgcatggcat ccacgcagag ccgacaacat tcggtctcaa actcgcttat gcctacgccg
      421 agttcgcgcg cgcgcgcgat cggctggccg ctgcgcgcaa ggaagtcgcc acctgcgcaa
      481 tttccggcgc ggtcggcacg ttcgcccagg tcgacccgcg cgttgaagcg cacgtcgcaa
      541 aagcgatggg cctgtccgtc gaaccggtgt ctacccagat catcccgcgc gaccggcacg
      601 ccatgtattt tgcaaccctc ggcgttgtcg cctcctcgat tgagcgcctt gcgaccgaaa
      661 tccgtcacct gcagcgcacc gaagtcctcg aggccgaaga atatttcacc gagaagcaaa
      721 aaggctcctc ggccatgccg cacaagcgca acccggtcct ttcggaaaac gtgaccggtc
      781 ttgcgcgcat ggttcgcgcc tatgcgctgc ccgccatgga aaacgtggcc ctctggcacg
      841 aacgtgacat ttcgcattct tcggttgaac gcatgatcgg cccggacgcc accgtcaccc
      901 ttgattttgc gctcgcgcgg ctggcggcga tcgtcgataa attgatcgtc tacccggcgg
      961 cgatgcagaa gaacctcgac aggcttggcg gactgatcca ttcgcagcgc gtactgatcg
     1021 cactcacaca aaaagggata gcgcgcgaag acgcctatcg cctcgtgcaa cgcaatgcca
     1081 tgaaggtctg ggcgggcgaa ggcgatttcc tgtcatttct gaaggccgat aaggacgtgc
     1141 gcgcgacgct caccgataga gagatcgagg ccaatttcaa cctcgaccac catttcaaac
     1201 acatcggcac catcttcgag cgggtattcg gatccgcctg atttctccga gcgcgatgcc
     1261 gcaagcaccc acaatattgc tgttcgactc cggtctcggg ggtttgaccg tttttcagca
     1321 agtgcggtcg gcgcggcctg acgcaaattt tgtttacgtg gcggatgacg ccgggtttcc
     1381 gtatggaaat ctcgaggagg atcggttgat tgagcgcgtc atcaaggtcg tcggacacgt
     1441 catctcggac agcaatcccg atcttgtcgt ggtggcctgc aacaccgcct ccaccctcgc
     1501 cctcggcgaa ttgcgcgcgc atttccgcgt ccctttcgtc ggcacggtgc ccgcgatcaa
     1561 gccggcatgc gcacagtcgg caacgaaacg catcgccgtg ctcggcacgc aggcaaccgt
     1621 tgcacgcgag tacacccaca cgctcatccg cgagttcggt tcaggttgtg acgtccacct
     1681 ggtcggctcg acgcagctcg ccgccagcgc agaagccgag ctcgccggca cgcctgtcgc
     1741 cgatgcggta attgccggtg aaatagcacg ctgcttcatc gacaaggatg gcaggcgcac
     1801 cgatacgatc gtgctcgcct gcacgcatta tccacttctg ctcgaacgtt ttcgcgcaaa
     1861 cgcaccctgg ccggtcgtct ggcttgatcc ggcgccggcc attgcccggc gggttgccga
     1921 cctcatgcgc gcccggccga acggaggtat gccgattgca cctcgtatga ttttcacatc
     1981 agggtgtgcg ccctcgccgc agctcgccgc gtcgctcgcc cgctacggct tctagccaaa
     2041 gatacgcggc tcgagttccc agtatccgcg ccagatcatg tcgagcgaaa catagagaat
     2101 gatcgcaagc ccggcatagg cgatccatcg gtgccggttc agcagccggg cgatgtagtt
     2161 cgcggcgact cccatcagta cgatcgagag ggcgagcccg aaaatcagtg cgtacggatg
     2221 gtcgcgcgcc gcacccgcta ctgcgagcac gttgtcgagc gacatggaaa cgtcggcaag
     2281 cacgatctgc caggcggcct gtgcaaaggt cttgcgcgga acgtgcgcct cccgcttcat
     2341 gagagcggct tcggtatctt ccgcgccggg accccgcaaa tcccgccaca tcttccagca
     2401 cacccagagc aacagaatgc cgcccgcgag cagaagcccg gtgattgcga gcagttccac
     2461 cgctatcgcc gcgaaggcga gacgcagcac ggtggccgcg atgatcccga cgaggatcgc
     2521 cttgcttcgt tgcgcgggcg gcaggccggc agccgcaaga ccgatcacca ccgcattgtc
     2581 gccggcgagt accaggtcaa tcatgatgac ctgcaggacg gcggcgatcg cgccctgcgg
     2641 aaccaaggtg agatcaatca ttggctctgt tcctgggccc cgacgggcgg atcaattcga
     2701 taatcggctc aatctctcct aaaatcgccg ctgcgcaacc gaccaaagtg aacgcacctg
     2761 ccatgtccca ggcagcgacg gcagaaggtc gtctggcgcg cggcgaaagg attgcgagga
     2821 gaaccgtgca ggccgcagca atgagtgccc acaggctgaa caacggcagc acgacagggt
     2881 gcggcaagat aaggacaaaa atggccagtg ccgcgaccaa taccgtgaca aacacaagac
     2941 cggacgcggg cggattttca cccggcgggg ggacgaagcg agggagctga gaaaaatctg
     3001 gtaaatgcat cgggcccgcc gaacgtcatc atcagtccga catcgcagcg acatgctgcc
     3061 agaggggccc gggctgatgc aggcaaacat gcttatcggc agccggaaat gcaagaaaaa
     3121 ccggctgcgg cgagataacc caggcccgca aggctcgttt gacaggccct tctgataccc
     3181 ctacaacccg cgcgcccggc ggatcctgga ggtttgccgg gcgtttcgcg acccgcggct
     3241 gggaccttgc gctttagagg tcctgcctgt cggttccggt tcctgccggg
//
LOCUS       DASSRL010000290         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_567752, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000290 DASSRL010000000
VERSION     DASSRL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567752"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFL49_05995"
     CDS             complement(<1..569)
                     /locus_tag="VFL49_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911560.1"
                     /translation="MSHNSLDDPAPPMLSDPGKLELVDLRRRLAARHPLSAPRIAGDF
                     HHKAGFRPDQPRWPKGTGEDSGRWSGGAGAEGNQGDAGTIDLSAMRRKPRLPELPLHL
                     PHFSSGPGRSSPSRQPITIINNAQTGFSTIDETTEKLRPILENVVNARPEGFGPDYGK
                     AIHYDFAAAVRSGNLRGIGSEGVEQTFGP"
     gene            807..1055
                     /locus_tag="VFL49_06000"
     CDS             807..1055
                     /locus_tag="VFL49_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911561.1"
                     /translation="MKHWPIGLLAAATGFFVASSAQAAPDFRVIQWDFTRICQIYDFG
                     WGGRPIPSNYRILTPPLPSFRAATRAKSRLARHGRCSI"
     gene            1176..1424
                     /locus_tag="VFL49_06005"
     CDS             1176..1424
                     /locus_tag="VFL49_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911562.1"
                     /translation="MKRFSLMLLGAVGAFFVLTPAQASDYRVIQWDTTRICQVYDMTW
                     KIKPFPSNYKVLTKKSLPTYAAAVEAKGKLAKKANCLI"
     gene            complement(1548..1880)
                     /locus_tag="VFL49_06010"
     CDS             complement(1548..1880)
                     /locus_tag="VFL49_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911563.1"
                     /translation="MIIATRTLFSRNNGRNIEIPIRIHVPEKAKVDWICRFEIEWPEG
                     RVERWGAGIDAIQSLWIALQMIGAEVYTSRHHEVGELVWLGPGRGYGFPVPNSIRDLL
                     VGDDKAHL"
     gene            complement(1967..2497)
                     /locus_tag="VFL49_06015"
     CDS             complement(1967..2497)
                     /locus_tag="VFL49_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911564.1"
                     /translation="MLLRMTLLCLYTTSVPSLAEVRDSFRDLLSVYRCEVVHRLEQIY
                     EFGDLANDRDRFIAITVPGHPHGYVQCMFHDNGTEIYCEASSGFYYDKENAPRTFRQP
                     QQAVDALAGLGFDTDDSEGNFRIDFAVGAPPNFNAIADFMLRALHDGYGARGQMNLKF
                     NAPFAPRAPSACVPVS"
     gene            2661..2966
                     /locus_tag="VFL49_06020"
     CDS             2661..2966
                     /locus_tag="VFL49_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7911565.1"
                     /translation="MAQRVSVIGAGTIGASWAAYFLARGFEVAAYDPTPNGEEFARHF
                     IDDAWPTLEKLNAVQPGADRRRFSFFKDSGLYCRCLPGRPLSVAAHSPRRLPSYLSP"
     gene            complement(2927..3178)
                     /locus_tag="VFL49_06025"
     CDS             complement(2927..3178)
                     /locus_tag="VFL49_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911566.1"
                     /translation="MAHQLYITNLDLLAPSVVKQLQNTLAGFPDWEIVVAVYLDEKGY
                     SWPDMGLIIRAHEIIDELQRQYFPVQYQGLKYDGSRRGE"
     gene            complement(3366..3869)
                     /locus_tag="VFL49_06030"
     CDS             complement(3366..3869)
                     /locus_tag="VFL49_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911567.1"
                     /translation="MAMISGLPPVVVQPADTLNELLAAERCSLSAQLKAVYERPSAFK
                     ERDRFLVVRVEARPQSYVQCMFADNRSKLYCEASSFYYAEPEMKPRTFYMPSDAMAAL
                     QKLGFATRTSEKSFPYERALSGTPDFDAIATLMLTALHDAYGAREDTVLTTAAPFVGT
                     IIVVCRR"
     gene            complement(3896..>4286)
                     /locus_tag="VFL49_06035"
     CDS             complement(3896..>4286)
                     /locus_tag="VFL49_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911568.1"
                     /translation="GFQLRLASDVRFATASGMVPATLEDKQTGFECYHDDAMETMGFL
                     GVSNFTHFWKYALGLRWGADFSALEAAWMAATAYAAATAGIIFDHEEGRVFTPQQARE
                     LVARFISDRPNTKAFLEDIERKFGKKP"
BASE COUNT          919 a         1243 c         1198 g          926 t
ORIGIN      
        1 tccggaccga atgtctgctc aacaccctcg ctgccgatcc cgcgcagatt tccggagcgc
       61 accgcggccg cgaaatcgta atggatcgcc tttccgtaat cgggaccgaa tccttctggg
      121 cgcgcattga cgacgttttc cagaatcggc cgcagctttt ccgtggtttc gtcgatcgtc
      181 gagaatccgg tttgcgcgtt gttgatgatc gtgatcggtt gccgcgacgg cgaactccgg
      241 cccgggcccg acgaaaaatg cggcaggtga aggggaagct cgggcagccg cggcttgcgc
      301 cgcatcgcgc tgagatcgat cgttccggca tcgccctggt tgccttccgc ccccgccccg
      361 ccggaccagc ggccgctatc ctcaccggtg cccttcggcc agcgcggctg gtcaggccgg
      421 aagcccgcct tgtgatgaaa gtcgccggcg attcgcggcg ccgaaagcgg gtgccgcgcg
      481 gcaagccgcc gccgcaggtc gacaagctcc agtttgccgg ggtccgaaag catcggcgga
      541 gcgggatcgt ccaggctgtt atgtgacatt tcggataatc tgccattgta ggtatattat
      601 cctataatcc cgcaatccga ttgtcaagta tttattccta atgttttgat tgtgagaaaa
      661 gcgcggcgcg ggcttactta cgacctccat gtgttacgga ccttgtcgag gcgccggcac
      721 ccggaacaat ttcccgcgcc cgcctgttag cgttgcggcg tgccccttcc ggagcaagcc
      781 ggattgaatc agacaggaga gcaggaatga agcactggcc gattggattg ctggccgccg
      841 cgacgggatt ttttgtcgca agctcggcgc aggcggcgcc tgacttccgc gtcatccagt
      901 gggacttcac cagaatttgc cagatctatg attttggctg gggcggccgg ccgatcccct
      961 ccaactaccg gatcctgacc ccgccattgc cgagcttccg cgcggcaacg cgtgccaaga
     1021 gcaggctggc gcgccacggc cgctgctcga tctaacaccg ccataaatat ccggagatta
     1081 tcgtgtaccg tgcaagtcac gtcttgcaag gcgctcgccg cacggcatac gcttttgcgc
     1141 cgtaacgcgg caggataaat ttcagggaga cgacgatgaa gcgtttcagt cttatgttgc
     1201 tgggcgccgt gggggcgttc tttgttctca ctccggcaca agcttccgat taccgggtca
     1261 ttcaatggga taccacgcga atctgccaag tctacgatat gacctggaag atcaagccgt
     1321 tcccgtcgaa ctataaagtt ctgacgaaaa aatcgctgcc gacctacgcc gctgcggtcg
     1381 aggcgaaagg caagcttgcg aagaaggcca attgcttgat ctagctgaca gtcaaggagc
     1441 cgggggcttc tgccacctca tcatcgccat caccgatgat gaggtggttt tgctgaggca
     1501 gagccggctg gctactcgac gcaagctggc tgctcacttg ttggcggcta caaatgggcc
     1561 ttgtcatccc ctacgagcaa atcacgaata ctgttcggca caggaaaacc atagccgcgg
     1621 ccaggcccga gccacacaag ttcgcccact tcgtgatgtc ggcttgtata gacttcagct
     1681 ccgatcatct gcagcgcaat ccacaaagac tgaatcgcat cgatcccagc gccccatcgt
     1741 tcaacgcgtc cttcgggcca ttcgatctcg aaacggcaaa tccaatccac tttcgccttc
     1801 tccggcacat ggatccgaat tggaatctca atattccggc cattattgcg ggagaataaa
     1861 gtacgagtag caataatcat ggcaaaacct caaaatggaa gtggtggaat tgctcgtccg
     1921 ctaagacatg cgcccgcacg catattttcc tattagtaag attgtgtcag ctgactggca
     1981 cacaggccga tggtgcgcgc ggtgcaaacg gagcattgaa tttcaagttc atctggcctc
     2041 gcgcgccata gccatcgtgt aaagcgcgca gcatgaaatc ggcaatggcg ttgaagttgg
     2101 gcggagctcc aacggcaaaa tcgatacgga agtttccttc cgaatcgtcc gtgtcgaagc
     2161 ctaggcctgc aagtgcatcg actgcctgtt gcggctgacg aaatgtacgc ggtgcatttt
     2221 ctttgtcgta gtaaaagcca gaagacgcct cacaatagat ttcggtgccg ttgtcgtgga
     2281 acatgcattg cacatagcca tgcgggtgac cgggcacggt gatagcgatg aaccggtcgc
     2341 gatcgttcgc caggtccccg aattcgtaaa tctgctcaag tcgatgcacg acctcacagc
     2401 gataaacaga cagaagatcg cggaaactgt cgcgcacttc cgcaagcgac ggtacgcttg
     2461 tcgtgtacaa acaaagcagc gtcattcgta acaacacgaa tatctcgata gattcatata
     2521 cacgctgcaa ttgtattatt ttatactacc cgcattagtc aagcgtgttt ttaactacgc
     2581 tgctgcaaga acctgcctgt gaggcgggta cgtgcagtgc agtctggtaa gctgtgccgc
     2641 acaagcgata gggaggacaa atggcacagc gcgtaagcgt cattggcgcc ggcacgatcg
     2701 gcgcaagctg ggcagcctat tttctggcgc gcggtttcga ggtcgccgcc tacgatccga
     2761 cgccgaacgg cgaggagttc gcccgccact tcatcgacga tgcctggcca acactggaaa
     2821 agctcaacgc ggtgcagcct ggcgccgatc gcaggcgctt ttcgttcttc aaggattcgg
     2881 gcctgtactg tcgttgcctt cccggtcgac cgctgtctgt ggccgctcac tctccgcggc
     2941 ggctgccgtc gtatttgagc ccttgatatt gcaccgggaa gtactggcgc tgcaattcat
     3001 cgatgatttc gtgcgcgcgg atgatcaaac ccatatcggg ccaagaatac cctttctcgt
     3061 cgagatagac ggctacaaca atttcccagt cgggaaagcc tgcgagggtg ttctgcagct
     3121 gcttcacgac cgagggcgca agcaaatcca gattggtgat gtacagctga tgagccaata
     3181 atccgcgcgt ccgcgccagt cttcccgacc aagatcgtcg agcagatccg ggcatagagt
     3241 atttcccaca ctcgcacctg ctcggcatcg tcgtcatcta cggtctgcat caaacgttcc
     3301 tcattcaact ctgcgcggaa ttcggcgcaa ataaaactgg aactcgcgca cacagatgct
     3361 gagccttatc tgcggcacac tacaatgatc gtgccgacga atggagccgc ggtcgtaagg
     3421 acggtatcct cccgcgcgcc ataagcatcg tgtagggctg tgagcatcaa ggtcgcaatc
     3481 gcgtcgaaat cgggcgtgcc gctgagagcg cgctcataag gaaaactctt ttccgacgtt
     3541 ctggtcgcga aaccaagctt ttgaagtgct gccatcgcgt cggatggcat gtagaacgtg
     3601 cgcggtttca tttccggctc ggcgtaataa aatgacgagg cttcgcaata aagtttgctg
     3661 cgattgtccg cgaacatgca ttgcacataa ctttgtggcc gggcctccac gcgaacgacg
     3721 agaaatcgat cgcgctcctt gaatgcgctc gggcgctcat agacagcctt tagttgcgcc
     3781 gatagcgaac agcgctcggc agccagcaac tcgttgagtg tgtctgctgg ctgcacaacc
     3841 acaggcggta gccccgagat catggccaag aaaactgcat gacggctttt gaccatcagg
     3901 gcttttttcc gaattttcgt tcgatgtcct cgagaaatgc tttggtgttg ggacggtcac
     3961 taataaatct ggcgaccagc tcacgtgcct gttgaggcgt gaacacccgt ccctcctcgt
     4021 gatcgaagat gatgccggcc gtggcagcgg cgtatgcggt tgcggccatc caggccgctt
     4081 ccaatgctga aaagtccgcg ccccagcgca agcccagtgc atatttccag aaatgggtga
     4141 aattgctgac gcccaggaac cccatcgttt ccatggcatc gtcgtgatag cactcgaacc
     4201 cggtttgctt atcctcgagt gttgccggca ccatgccgct tgcggtggcg aaacgaacat
     4261 cgctggccaa ccgcagctga aagcct
//
LOCUS       DASSRL010000291         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1215275, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000291 DASSRL010000000
VERSION     DASSRL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215275"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1234)
                     /locus_tag="VFL49_06040"
     CDS             complement(59..1234)
                     /locus_tag="VFL49_06040"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7911569.1"
                     /translation="MKDDIVIVSAARTPVGAFNGAFANLPAHELGKAAIKAAIERAGV
                     EGGRVSEVILGQILTAGQGQNPARQASIAAAIPVETPAWGVNQLCGSGLRAVALGYQA
                     ILNGDSEIVVAGGQESMSMAPHCQHLRSGVKMGSFEMIDTMIKDGLWDAFNGYHMGTT
                     AENVAKQWQITRQQQDEFAVASQNKAEAAQKSGKFKDEITPVTIKSRKGDIVVDTDEY
                     PKSGVTMESIAKLRPAFDKEGTVTAANASGINDGAAAVVLMKASEAAKLGKTPLARIV
                     SWAQAGVDPAIMGTGPIPASRAALKKAGWKKEDLDLVEANEAFAAQACAVNKDLGWDT
                     SKVNVNGGAIAIGHPIGASGARVLVTLLYEMQKRNAKKGLATLCIGGGMGIAMCVER"
     gene            1485..2066
                     /gene="phaR"
                     /locus_tag="VFL49_06045"
     CDS             1485..2066
                     /gene="phaR"
                     /locus_tag="VFL49_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HET7911570.1"
                     /translation="MSEDKKEPVTIKKYANRRLYNTGTSTYVTLEDLAAMVKNGEDFI
                     VYDAKTGEDITRSVLAQIIFEQENKEGQSLLPITFLRQLIRFYGDSMQMLVPRFLEAS
                     IESLTKEQGKFREQMAQAFGVGAFGPLEDQVRRNMEMFEKTFAMFAPFARGQSKPAEK
                     AQGTGGGSGNEMDDLKRQLDEMQKRLDKLGGKE"
     gene            complement(2554..>2996)
                     /locus_tag="VFL49_06050"
     CDS             complement(2554..>2996)
                     /locus_tag="VFL49_06050"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7911571.1"
                     /translation="GIMLNNRGSCFTLEPDHPNTFGPGKRPMHTIIPGLTMRDGRCDM
                     SFGVMGAHYQPMGHVQIILNMLDYGMDVQQAIDAPRFFFEGEKTDVERGTPAATIEGL
                     KARGHDVVMATSPWGGAQTIRIDWGRGVLIAGSEPRKDGCALGY"
BASE COUNT          589 a          917 c          864 g          626 t
ORIGIN      
        1 catagccgtg caaagcacgc cgtccggcca tgttgtgaat caatcggcag caccaaggct
       61 accgctcgac acacatggcg atgcccatgc cgccgccgat gcaaagcgtg gccaaaccct
      121 tcttggcatt acgcttctgc atttcataga gcagcgtcac caacacgcgc gcgcctgaag
      181 cgccgatcgg gtgtccgata gcgattgcac cgccgttgac gttcactttc gaagtatccc
      241 agccgagatc tttgttgacc gcgcaggcct gcgcggcgaa tgcctcgttc gcctcgacga
      301 ggtcgaggtc ttcctttttc cagccggcct tcttaagcgc cgcccgcgac gccgggattg
      361 ggcccgttcc catgatggcc ggatcgacgc ccgcctgcgc ccaggaaacg atgcgtgcaa
      421 gcggggtctt gccgagcttc gctgcctccg aagccttcat cagcaccaca gccgccgcgc
      481 cgtcgttgat gcccgacgca ttagctgcgg ttaccgtgcc ttccttgtcg aaggcagggc
      541 gtaacttggc aatcgactcc atcgtcacgc ccgattttgg atactcgtcg gtatccacga
      601 cgatatcgcc cttgcgtgac ttgatcgtca caggtgtgat ttcgtccttg aacttgcctg
      661 acttttgggc ggcctctgcc ttgttttgcg aagcgaccgc aaactcgtcc tgctgctggc
      721 gcgtgatctg ccattgcttg gcgacgttct ccgccgtcgt tcccatgtgg tagccgttaa
      781 aggcgtccca caggccatcc ttgatcatgg tgtcgatcat ctcgaacgac cccattttca
      841 cgccggaacg caaatgctgg cagtgcggcg ccatgctcat cgattcctgg ccgccggcta
      901 caacaatttc agaatcgccg ttgaggattg cttgatagcc gagcgcgacc gcgcgcaggc
      961 cggaaccgca tagctggttt acgccccacg caggagtctc gaccggaata gctgcggcga
     1021 tcgatgcctg ccgcgccggg ttctgtccct gccccgccgt aaggatctga cccaggatga
     1081 cttcactgac gcggccgcct tcgactcctg cccgctctat cgcggccttg atcgcagcct
     1141 tccccagctc atgtgccgga agattcgcaa atgccccgtt gaaagcgccg accggggtcc
     1201 gggccgcgct cacgatgacg atgtcgtctt tcatcagaag ctcctgttag atcagactct
     1261 ggggcattca ccggatcttt cggcactgcc gcatcctcag taggccaagc tcgtctgtca
     1321 actcgacctg ccactcgaat attcagacaa ttggcgcttt ttcgaccctt ctgcaagaga
     1381 tgcccgaagc cgttccggca ctgccgcggt gccacaaaac ggtggcccag cgaagcggaa
     1441 attggttatg cttgaagcct gggcagggga cacgtgtgaa cgatatgtca gaggacaaga
     1501 aagaaccggt cacaataaag aagtatgcca accggcggct ctacaatacc ggaacaagca
     1561 cttacgtgac gctcgaggac ctcgccgcca tggtcaaaaa tggcgaggat ttcatcgtct
     1621 acgacgccaa gaccggcgag gacatcacgc gctcggtcct tgcgcaaatc atctttgaac
     1681 aggaaaacaa agaaggtcag agcctgctgc cgatcacatt tctccggcag ctgatccgct
     1741 tttatggcga cagcatgcaa atgctcgttc cgaggttcct cgaagcttcg atcgaatcgc
     1801 tgacgaaaga gcagggaaag tttcgcgaac agatggctca ggcctttggc gttggggctt
     1861 ttggtcctct cgaggatcaa gtccgtcgca acatggaaat gttcgaaaag actttcgcca
     1921 tgttcgcgcc ttttgcgcgc ggccaatcga agcccgccga aaaggcgcaa ggtacgggcg
     1981 gcggatcggg caacgagatg gacgatctca aacgtcagct cgacgaaatg cagaagcggt
     2041 tggacaagct cggcggcaag gaatagtcgg tggcccgtta ccgcatcgtt ttggaacaca
     2101 aggacgtgtg aagacgtttc tttgcgcgac ggatgtgcgc tagcgtccag ccgcatttcg
     2161 tcagaccttt caaaaggtgg gattttgaag gctgggccgc ggttaacagc gcgcggcacg
     2221 gcgtttgcac gtttgagttt tggcagaagt cgagtgtcct gaatcgagac gacagatgcg
     2281 gcttggcgac gttaacgaat cacaattccg gcagacccct cctgaggcgg gtgtgtccgc
     2341 gactggcggc gtgctccctg gatcggagag ccgtgcgctc gtcgcgctcg ctccgttgca
     2401 aagtgtctcc ggacaaccgg ctggccatcg ccatgcacca tttgtcgcgc atctggttgc
     2461 aacaaaagat cagcatccgc agacgcgtga acgtcggcgc gcggatccac acgaggcctt
     2521 ggcggcctat cgggctgcgg ctgctctgac gatttaatag ccgagcgcgc agccgtcctt
     2581 gcgcggctcc gagccggcga tcaatacacc gcgaccccag tcgatcctga tcgtctgcgc
     2641 gccgccccac ggcgacgttg ccatgacgac atcatgcccg cgcgccttca ggccttcgat
     2701 tgttgccgcg ggcgtgcctc gctcgacgtc ggttttttcg ccttcaaaga agaagcgtgg
     2761 tgcgtcgatc gcctgctgca catccattcc gtaatcgagc atgttgagaa tgatctggac
     2821 atgacccatg ggctgatagt gcgcacccat cacaccgaag ctcatgtcgc agcggccatc
     2881 gcgcatcgta aggcccggga tgatcgtgtg catcgggcgc ttgcccggac cgaacgtgtt
     2941 tggatggtcc ggttcgagtg taaagcatga gccgcggttg ttcagcatga taccgg
//
LOCUS       DASSRL010000292         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_865189, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000292 DASSRL010000000
VERSION     DASSRL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865189"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..590
                     /gene="rsfS"
                     /locus_tag="VFL49_06055"
     CDS             183..590
                     /gene="rsfS"
                     /locus_tag="VFL49_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7911572.1"
                     /translation="MSTSALSRAPRRKPETVSRHPTAKETLKTVLARLEDMKAEDSVV
                     IDLTGKSSIGDYMVVTSGRSQRHVSSMADHVVKDIEEAGGPRARVEGMRQGDWVLIDA
                     GDVIVHVFRPEIRAFYNLEKMWAAASRDARRPG"
     gene            607..1089
                     /gene="rlmH"
                     /locus_tag="VFL49_06060"
     CDS             607..1089
                     /gene="rlmH"
                     /locus_tag="VFL49_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET7911573.1"
                     /translation="MRIIVAAVGRLKQGPETELSSRYRKRAAQTGRHLGLRDVEIIEI
                     RESRAGDAAKRMLEESIALANVIPQNAVVVVLDSRGESLDSGALAAQIAKWRESSRPA
                     VVFLIGGADGLAVNLGEKASLRLSFGMATWPHQLVRVMLLEQIYRVTTILTGHPYHRA
                     "
     gene            1193..2530
                     /locus_tag="VFL49_06065"
     CDS             1193..2530
                     /locus_tag="VFL49_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7911574.1"
                     /translation="MICLGGFGGFDPRIALIAGLGFALPVTLAAAVLAQERPVPAAQN
                     ETADPLRTLKQRDEELKAVRDEQRKSAESESALKREIEQIGADRTKLSQDLIDTAGRL
                     RAVEAKVASTQERLKPLDENERSIRKSLEGRRAVIGEVLAALQRMGRQPPPALVASPE
                     DALQSVRTAIVLGAVLPQMRREVESLANDLSELLAVRKKIEVEREQLKTELAALDGER
                     ARMAALVDERQKQLAEREKALEAERARAGALAHQVDDLKDLIAKLEQGLDPKTRELRE
                     AGRSDSRPALSAFRDPGRLAPAVAFASLRGRVPIPVNGVKLREFGAPDANGGSEKGIS
                     IATRTGAQVTAPADGWVVYAGPFRSYGQLLILNVGGGYHVLLAGMDRISVDLGQFVLT
                     GEPVAVMGNGSHIAATRATGSSQAVLYVEFRKDGIPVDPGPWWAAGEGEKVRG"
     gene            2527..>2895
                     /locus_tag="VFL49_06070"
     CDS             2527..>2895
                     /locus_tag="VFL49_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HET7911575.1"
                     /translation="MMRKTSLILLGAAAGAAMTLLAVQPRMVLVGATAKAAAADTYRQ
                     LNLFGDVFERVRADYVEKPDDAKLIETAINGMLAGLDPHSSYMDAKSFRDMQVQTRGE
                     FGGLGIEVTMEDGLIKVVAPI"
BASE COUNT          598 a          853 c          931 g          513 t
ORIGIN      
        1 gaatcgaaac gggcacggaa cggcggcaaa cccgaaaaca agcgtggaaa cagaaccagc
       61 aaacgcaaat cggcggcgaa gttcgcgcgc cgccggggta gaaagacgcg aacgagctga
      121 ttatcttcag gtgaatcccg atagtatcgg gtgagtgcga cggatcgaaa ggaccagaaa
      181 ccctgagcac atctgcactc tctcgggcgc cgcgtcgtaa gcccgaaacc gtctcccggc
      241 atccgactgc caaggagacg ctcaaaaccg tcctcgcgcg tttggaagat atgaaggccg
      301 aggacagcgt cgtcatcgat ctcaccggca aatcctcaat aggcgattac atggtcgtga
      361 cgagcggccg gtcgcagcgt cacgtcagct ccatggccga tcacgtcgtc aaagacattg
      421 aggaagccgg cggcccccgc gcccgcgtcg aaggaatgcg gcagggagat tgggtcctca
      481 tagacgccgg tgacgtcatc gtgcatgtct tccggccgga aattcgcgcc ttctacaatc
      541 tggaaaagat gtgggcggcg gccagcaggg acgcacggcg gccaggctga tagtctgcgc
      601 ggcaacatgc gcatcatcgt tgccgccgta gggcgtctga agcagggccc ggaaaccgaa
      661 ttgagttcgc gctatcgtaa gcgcgccgcg caaaccggcc gtcacctcgg cctgcgcgac
      721 gttgaaatca tcgaaatccg ggaaagccgt gccggcgacg ccgcaaagcg catgctcgaa
      781 gaatcgatcg cgctcgccaa cgtcattccg cagaacgcag tcgtggtcgt gctcgattcg
      841 agaggagaaa gtctcgacag cggtgcgctc gccgcccaga tcgccaaatg gcgggaaagc
      901 agccggcctg cggtggtctt cctgatcggg ggagccgacg gcttggctgt gaaccttggc
      961 gaaaaagcca gcctccggct gtcgtttggc atggcgacct ggccgcacca gctcgtccgt
     1021 gtaatgcttc ttgagcagat ctatcgggtc accacgatcc tgaccgggca cccttatcat
     1081 cgagcctgaa ttcgcgggcg gagtggatcg accccgattt agtcgcaatc tgatccgata
     1141 ggttttggca aatgaacttg tcgttcaaac ttgcgggcgt atcgtggccc cgatgatttg
     1201 ccttggggga ttcggcggtt ttgatccgcg cattgcactc atcgcggggc tgggatttgc
     1261 gttaccggtg actctcgccg ccgcagtgct ggcgcaggaa cggccggttc ccgctgccca
     1321 aaacgagacg gcagatccgc tcaggacact caaacagcgc gacgaagagc tcaaagccgt
     1381 tcgtgacgag caacgcaaat ccgcagagtc ggaatccgcg ctcaagcgcg agatcgagca
     1441 gatcggcgct gatcgcacga aactcagtca ggatctgatc gacactgccg gacgcttgcg
     1501 cgccgtcgaa gccaaagttg cgtcaaccca ggaacggctc aaaccgctcg atgaaaatga
     1561 acgcagcatt cgaaagtctc tcgaggggcg tcgcgcggtg attggagagg tcctggccgc
     1621 gctgcaacga atgggtcgtc agccgccacc ggcgcttgtc gccagccccg aagacgcgtt
     1681 gcaatcagtg cggacggcca tcgtgctggg cgctgtcctg ccgcaaatgc gcagggaagt
     1741 ggaatcgctg gcgaacgatc tgtccgaatt actggcggtg cggaaaaaga tcgaagtcga
     1801 gcgcgagcag cttaagaccg aacttgccgc gcttgatggc gaacgcgcac gcatggcggc
     1861 actggtggat gagcgtcaaa aacaattggc cgaacgcgag aaggcacttg aggccgagcg
     1921 ggcccgagcg ggcgcgctgg cgcatcaggt cgacgatctc aaggatttga tcgccaagct
     1981 cgagcagggg ctcgacccca aaacgcgcga gttgcgcgag gctggccgct ccgacagccg
     2041 tccggcgctt tcagcatttc gtgatccggg acgccttgcg cctgccgttg ctttcgcgtc
     2101 tttgcgcggg cgggttccga ttccggttaa cggcgttaaa ttgagggaat ttggtgcgcc
     2161 agacgccaat ggcggctcgg aaaaaggcat ttccattgcc acgcgtaccg gcgcgcaggt
     2221 gaccgccccg gccgacggtt gggtcgtcta tgccggtccg ttccgttcct acggtcaact
     2281 cttgatcctt aatgtcggcg gcgggtatca tgtcttgctt gccgggatgg atcggatatc
     2341 agttgacctc gggcagtttg tgctgaccgg ggaaccggtt gcagtcatgg gtaacggctc
     2401 tcatatcgcc gctacccgcg cgacaggttc cagccaagcg gtgctctacg tcgagtttcg
     2461 aaaggacggg attcccgtcg atccaggccc atggtgggca gctggcgaag gcgaaaaggt
     2521 tcgcggatga tgcgcaagac ctcactcatt cttcttggcg cggcggcggg agcagcaatg
     2581 acgctgctcg cggttcagcc gcgcatggtg ctcgtgggcg cgaccgccaa ggcggcggca
     2641 gcggatacgt atcgccagct taatctgttc ggcgacgtct tcgagcgcgt gcgcgccgac
     2701 tacgtcgaga agcccgatga cgcaaaactt atcgaaaccg cgatcaacgg aatgctcgcc
     2761 ggcctcgatc cgcattcgag ctacatggac gccaagagct tccgcgacat gcaggtgcag
     2821 acgcgcggcg agttcggcgg gctgggcatc gaggtcacca tggaggacgg cctcatcaag
     2881 gtggtcgcac cgatc
//
LOCUS       DASSRL010000293         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_847687, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000293 DASSRL010000000
VERSION     DASSRL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847687"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFL49_06075"
     CDS             complement(<1..390)
                     /locus_tag="VFL49_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7911576.1"
                     /translation="MTVELTKTPSFRLDGKRALVTGAGRGIGLSAASALADAGAHVTL
                     AARTSNEIEEAADAIRARGQKAESLTLDVRNVEAVKTMIVAEAPFDILVNNAGTNKPA
                     SFVDVTVEDFDFVLSLNVRAAYFVAQAV"
     gene            complement(387..1694)
                     /gene="hisD"
                     /locus_tag="VFL49_06080"
     CDS             complement(387..1694)
                     /gene="hisD"
                     /locus_tag="VFL49_06080"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET7911577.1"
                     /translation="MARWLKRGMDAGAIKAADAKVRETVESILAHVEERKDDAIRELS
                     EKFDKWSPKDFRLSPSEVERAITQVPKRDIEDIKFAQAQVRNFAQKQRDTMLDLEVET
                     LPGVVLGHRHIPVNSIGCYVPGGRYPMVASAHMSIVTAKVAGVKRIIACAPPFKGGPH
                     PAIVAAMHFGGADDIYVLGGVQAVAAMALGTESIKPVDMIVGPGNAYVAEAKRQLFGR
                     VGIDLLAGPTETLIIADDSVDGEICATDLLGQAEHGPTSPAVLITNSEKLARDTMKEV
                     ERLLKILPSADLAGQAWKDYGEVIVCGDEAEMIVEADRVASEHVQVMTRDPDVFLNGM
                     KNYGSLFLGPRTNVAYGDKVIGTNHTLPTRKNARFTGGLWVGKFIKTCTYQKVLTDEA
                     SARIGEVCSRLCVLEGFLAHAEQANVRVRRYGGRNVPYAQAAE"
     gene            complement(1827..2780)
                     /locus_tag="VFL49_06085"
     CDS             complement(1827..2780)
                     /locus_tag="VFL49_06085"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7911578.1"
                     /translation="MNIKVGKQAIAEATQKLKNWGRWGKEDQIGTLNHVTPEDIVKAA
                     SLIRSGKVFALGIPLDRTGPQTGLFGGRFNPIHQMLATGTDAIAGQQDWNKIRYADDT
                     LTLCVQGATHWDALGHIFYEEKAYNGYDAKLIDSRGLSVLGIEHSKNKMVGRGVLLDI
                     ARFRGVKWMQDGESISNDELDQCAKQQNVSIGRGDFVIIRTGQMERCHEEKDWGGYAG
                     GDAPGVKFENCYWCQEKQVAAICTDTWGVEVRPNETIEANQPWHWVVIPAMGLCMGEI
                     FYLKELAEDCEQDGIYEFFFCGPPLIITGGTGSPINPQAIK"
     gene            2974..>3451
                     /locus_tag="VFL49_06090"
     CDS             2974..>3451
                     /locus_tag="VFL49_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7911579.1"
                     /translation="MKRNLIATAAVVAALTLPARANNIVDEWASIKAPAAPTLKPVTV
                     DPKTTALLMLDFMNQNCGKRPRCLATIPAMKRLLDEARAHKVAVIYSIIANSTPGDVI
                     KDVAPQAGEPHVLSGVDKFLNTDLDKILKDKGIKTVIAVGTSSNGAVLYTASGAASR"
BASE COUNT          635 a         1073 c         1033 g          710 t
ORIGIN      
        1 gaccgcctgc gccacgaagt aggcggcgcg aacgttgagt gaaagtacga agtcgaaatc
       61 ctcgactgtc acatcgacga acgacgccgg tttgttggtg ccggcgttgt tgacaaggat
      121 gtcgaatggc gcctctgcga ctatcattgt tttgacggct tccacgttac gcacatcaag
      181 cgtgagcgac tcggccttct gtccgcgcgc gcggatggcg tctgcggcct cctcgatctc
      241 attcgaggtg cgtgcggcga gcgtgacatg cgcgccggca tcggccagcg ccgaagcggc
      301 cgacaatccg atgccgcgac cggcgccggt caccagcgcg cgcttaccgt cgagacggaa
      361 cgaaggagtc ttcgtcagct cgaccgtcat tccgccgcct gcgcgtacgg cacgttgcgg
      421 ccgccgtagc ggcgcacgcg cacattggcc tgctcggcgt gcgccaggaa gccctccagc
      481 acgcaaagcc gcgaacacac ttcaccgatc ctggcgctgg cctcgtcggt cagcaccttc
      541 tggtaggtgc aagttttgat gaatttgccg acccatagcc cgccggtgaa acgcgcattc
      601 ttcctggtgg gcagcgtatg attggtgccg atcaccttgt cgccgtaggc gacattggtg
      661 cgcgggccga ggaacagcga gccgtagttt ttcatgccgt tgagaaagac atccggatcg
      721 cgggtcatca cctgcacatg ctccgatgcg acacggtcgg cctcgacgat catctcggcc
      781 tcgtcgccgc agacaatgac ttcgccatag tccttccatg cctggcctgc gaggtccgcg
      841 ctcggcagaa ttttcaatag ccgctccact tctttcattg tgtcgcgcgc gagcttttcc
      901 gaattggtga tgaggacggc aggagaggtg ggtccatgct cggcctggcc gagcaggtcg
      961 gtggcacaga tttcgccatc gacactgtcg tccgcaatga tcagcgtttc ggtcggtccg
     1021 gcgagcagat ctatgccgac acgcccgaac agctggcgtt tggcctccgc cacgtaggca
     1081 tttcccgggc cgacgatcat gtcgaccggc ttgatgctct ccgtgccgag cgccattgcg
     1141 gccaccgcct gcacgccgcc caatacatat atgtcgtcgg cgccaccgaa gtgcatcgcc
     1201 gcgacgattg ccgggtgcgg cccgcctttg aacggcggtg cacaggcgat gatacgttta
     1261 acgcccgcga ccttggccgt gacaatcgac atgtgcgcgc tcgccaccat cggatagcgg
     1321 ccgcccggca cgtagcagcc gatcgaattg accgggatgt gacgatggcc gagcacgacg
     1381 ccgggcaggg tttcgacctc aagatcgagc atggtgtcac gctgcttttg cgcgaaattg
     1441 cgcacctggg cctgcgcgaa cttgatgtcc tcgatgtccc gtttcggcac ctgggtgatg
     1501 gcgcgctcga cttcggatgg cgagaggcgg aagtccttcg gcgaccactt gtcgaacttc
     1561 tccgataatt cgcggatcgc gtcgtccttc cgctcctcga cgtgggcgag aatggattcc
     1621 acggtctcgc gaactttggc gtccgcggcc ttgatcgcac ccgcgtccat tccgcgtttg
     1681 agccaacgag ccatggtgtc ctccggtctg tccgcatcat ggccgggctt gacccggcca
     1741 tccacgtctt cgccagcact ataacctaaa gacgtggacc cccgggacaa gcccggggat
     1801 gacagagatt ggcgcgatgt tggcgattat ttaattgcct gcggattgat cggcgagcca
     1861 gttccgccgg tgatgatcag cggcgggccg caaaagaaaa actcatagat gccgtcctgc
     1921 tcgcagtcct cggcgagttc cttgagatag aaaatctcgc ccatgcagag gcccattgcc
     1981 gggatcacga cccaatgcca gggctggttg gcttcgatgg tttcgttggg gcgcacttca
     2041 acaccccagg tgtcggtgca gatcgcggcg acctgcttct cctgacacca gtagcaattc
     2101 tcgaatttga cacccggcgc gtcgccgccg gcatagccgc cccaatcttt ctcctcgtgg
     2161 cagcgctcca tctgcccggt gcggatgatg acgaaatctc cgcgcccgat cgaaacattc
     2221 tgctgcttgg cgcattgatc gagttcgtcg ttggaaatgc tctcgccatc ctgcatccac
     2281 ttcacgccgc ggaagcgcgc gatgtcgagc agcacgccgc ggccaaccat tttgttttta
     2341 gaatgctcga ttccgagcac ggacaggccg cgcgagtcga taagcttggc gtcgtagcca
     2401 ttataagcct tctcttcgta gaaaatgtgc ccaagcgcat cccaatgggt ggcgccttgc
     2461 acgcacagag tcagagtgtc gtcggcataa cggatcttgt tccagtcctg ctggccggct
     2521 atcgcgtcgg tgccggtagc gagcatctgg tggatcgggt tgaagcgccc gccaaataat
     2581 ccggtctgcg ggccagtgcg gtcgagcgga atgccgagcg caaagacctt tccggagcgg
     2641 atcaggctcg ccgccttcac gatatcctcg ggcgttacgt ggttgagcgt gccgatctgg
     2701 tcctctttgc cccaacgccc ccagttcttg agcttctgag tggcttcggc gatcgcttgc
     2761 ttgccgacct tgatgttcat aagcctgttc cttccggtct tttcgttcgc ggactgctct
     2821 tatcaacgct gccgtactcg ttgcaatatt gcgctggacc ccggtgtttc atcaagcggg
     2881 gtgcgttgcc gcagctttga cagaacacgc ggtgcttgcg catgatcgtg cgcacaagca
     2941 tctgctcgag aagctcaccc agggaggata ttcatgaaac gcaatctgat tgccacggcc
     3001 gccgtggtgg cggcgctgac gttgcccgcg cgggcaaaca atatcgtcga tgagtgggca
     3061 agcataaagg cgccggcggc gccaacactg aagccggtaa ccgtcgaccc caaaaccacg
     3121 gctctcctga tgcttgattt catgaatcag aactgcggca agcgcccgcg ctgcctcgcg
     3181 acgattccag cgatgaagag gcttttggat gaggcgcgtg cacacaaggt cgcagtgatc
     3241 tattccatca tcgccaatag tacgccaggc gacgtgatca aagatgtggc gccgcaagcc
     3301 ggcgagccgc acgtgctgtc gggcgtcgac aaattcctga acactgacct cgacaagatc
     3361 ctcaaagaca aaggcatcaa gaccgtgatt gccgtcggga cctcatcaaa cggcgcggtg
     3421 ctctatacgg caagcggagc ggcgtcgcgc g
//
LOCUS       DASSRL010000294         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_445452, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000294 DASSRL010000000
VERSION     DASSRL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445452"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..921)
                     /locus_tag="VFL49_06095"
     CDS             complement(52..921)
                     /locus_tag="VFL49_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7911580.1"
                     /translation="MRSLLFVPADGGPKLDKALASGADAVIVDLEDSIVPERKPAARA
                     AALEFLKRAGAAKRRPRLLVRINGLDTGMTDADLDAIVAGKPDAVVFPKAEGGPSVIH
                     LDAKLTAREAIAGVPEGQIKILAQAVESAAGLFAAGNFRNVSARLIGLTWGPEDLSAE
                     LGAETNRDLQGELTEPYRLARSMCLYAAAAAKVPAIETVYVDFRNSEALRRDTEAARR
                     DGFTGRLAIHPAQVAVINDVFTPTAAQIAKAKAVVAAFAAQPGAGAVGIDGKMYDRPH
                     LARAQALLASAKD"
     gene            1038..1934
                     /locus_tag="VFL49_06100"
     CDS             1038..1934
                     /locus_tag="VFL49_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7911581.1"
                     /translation="MQIRSLIALSAAVGAAGLATTAMAQTPAQQPMFATTKVDGTDNV
                     YIFRYQNHQAMFVVTPAGVIVTDPISYGRPQAAKTYLDEIRKITNAPIKYLIYSHHHY
                     DHIAGGKVFKDAGATVVAHRRAKERLAQLKGLDVVIPDQVVDGKRTIKLGGTALDLIY
                     TGRNHSDSSIVMLLPKEKILFAVDFNSLGAVPSRLAVNDSYPTEWEASLQRTLALNWE
                     RMIPGHPGPGGRLGTKKDMEQQIAFMTDMAAEVKKASDAGKCFDPATKDVRLPKYGTL
                     TGYEQNIEWNIHRWCGYYGRGI"
     gene            complement(1979..>2167)
                     /locus_tag="VFL49_06105"
     CDS             complement(1979..>2167)
                     /locus_tag="VFL49_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7911582.1"
                     /translation="VKFPNPLFEGDTIHCSTQVVSKRESKSRPGAGIVEFHHQAFKQD
                     GTLVAECHRQAFMRMRPK"
BASE COUNT          423 a          657 c          669 g          418 t
ORIGIN      
        1 tgacgctgaa aagcatttcg atccgaacaa agtctacaaa tagccgaccg ttcagtcttt
       61 cgcgctcgcc aacaacgcct gtgcacgggc gaggtgcgga cgatcgtaca tctttccgtc
      121 gatgccgacc gcgccggcgc caggctgtgc cgcaaaggcc gcgaccaccg ccttggcctt
      181 tgcgatctgc gccgcagtcg gcgtgaacac gtcgttgatc accgccacct gagcggggtg
      241 aatggcgaga cgtcccgtga aaccatcgcg gcgcgcggct tcggtgtcgc ggcgcaaggc
      301 ttccgagttg cgaaagtcga cgtagacagt ttcgatggcc ggcacctttg ccgccgcggc
      361 tgcatagagg cacattgagc gcgcgagacg gtatggctcg gtcagttcgc cctgcaggtc
      421 gcgattggtt tccgcgccca gttctgccga aagatcttcc gggccccatg tgagtccgat
      481 caggcgtgcg ctgacgttgc gaaaattgcc ggcggcaaaa agtccggccg ccgattcaac
      541 ggcttgtgcc aaaatcttga tctgtccttc gggcacgccg gcaatggcct cgcgggcggt
      601 gagctttgcg tcgagatgga tcacgcttgg gccgccttcg gccttcggga aaacgacggc
      661 atccggcttg ccggcgacga ttgcgtcgag atcggcatcg gtcatgcccg tatcgagacc
      721 gttgatgcgc acgagaaggc gggggcgccg tttggcggcg ccggcgcgct tgaggaattc
      781 cagcgccgcc gcacgggccg ccggtttgcg ctcgggaacg attgaatctt ccaagtctac
      841 aatcacggcg tcggccccgc tcgccagtgc cttgtcgagc ttggggccgc cgtcggcggg
      901 aacgaacaaa agagagcgca tgtaccggtc cttgttgggc tttttgaact tagccgaaat
      961 accgcccggg tgcgcgattt tggcgcagga attagcgccg gtcttgcggg gtttgggtcc
     1021 cgggggaggt accacccatg caaatcagga gtctcatagc gctttcggca gctgtcggtg
     1081 ctgccggttt agccacgact gcaatggcgc agacaccggc gcaacagccg atgtttgcca
     1141 ccaccaaagt cgacggcacc gataacgtct acattttccg ttaccaaaat catcaggcga
     1201 tgttcgttgt cacgcctgcc ggcgtgatcg tcacagatcc gatcagttac ggccgtccgc
     1261 aggcggcgaa gacttatctg gacgagatcc gcaagatcac caacgcgccg atcaaatatc
     1321 tgatttacag ccatcatcac tatgaccaca tcgccggcgg caaagtgttc aaggatgcag
     1381 gggctacggt ggtcgcgcat cgccgcgcca aggagcggct ggcccagctc aagggccttg
     1441 acgttgtcat tccggaccag gtcgtggacg gcaagcgtac gatcaagctt ggcggcaccg
     1501 cgctcgatct catctatacc ggccgcaatc attcagactc atcgatcgtc atgctgctgc
     1561 cgaaagagaa gatcctgttc gccgtcgact tcaattccct cggcgcagtg ccgtcgcgcc
     1621 tcgctgtgaa cgattcttac ccgacagaat gggaagcatc attgcagcgc accctggctc
     1681 tgaattggga gcgcatgatt ccgggtcacc ccggaccggg tggccggctt ggcaccaaga
     1741 aggacatgga gcagcagatc gccttcatga ccgacatggc cgccgaggtg aaaaaagcgt
     1801 cggacgccgg caaatgtttc gacccggcaa cgaaggacgt gcggctgcca aaatatggaa
     1861 cgctcacggg ttacgagcag aacatcgagt ggaacattca ccgctggtgc ggctattacg
     1921 gccgcggcat ctgatccgcc caggaaccca ccggagtgac tttcaggccc ggccggtttt
     1981 atttcggccg catgcgcatg aaggcctgcc ggtgacattc cgccaccagc gtgccgtcct
     2041 gcttgaaggc ctggtgatgg aattcgacga tcccggcgcc agggcgcgac ttcgactcgc
     2101 gcttgctcac cacctgcgtg gagcaatgga ttgtgtcgcc ttcaaagagg ggattgggaa
     2161 acttcac
//
LOCUS       DASSRL010000295         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1320321, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000295 DASSRL010000000
VERSION     DASSRL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320321"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="VFL49_06110"
     CDS             <1..1699
                     /locus_tag="VFL49_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7911583.1"
                     /translation="VESANGARWISWREVAVRSDGGTEVQSVGRDVTSRVEAEQALTV
                     ARDQAEAASRTKSRFLATVSHEIRTPLNGMLGMADLLLDTPLTSEQLTYTKAAKASGE
                     TLLALIEEILDFSKIEAGRLDVELRPFALAAMIEESVELMAPRAQAKGIEIVSYIDER
                     VPANVVGDAARLRQVLLNLIGNAVKFTEKGGVGVVVEPGEYENEIRFEVRDTGIGIKQ
                     DDQQRIFDDFEQADGSSTRKFGGTGLGLAISKRILERMNGRIDVTSQVGVGSIFGISL
                     RLPPADTQEAPFVAPELNRLAVMIVASAEIEASLLARRLGRWGASTCAVIDETIAAAL
                     LPERPWDALLVDYPLARAMAENRDLNRLRIARRIVMIRPSERHELPALKKAGFTGYLV
                     KPVRAASLASRLTASDIFEYSANDAANETAEALSPSVKGLSILVAEDNEINALLARAL
                     LTRLGHRPTIAATGEMAVEFWLAARAAGSRYDLVLVDLQMPGLDGLEAARRIRAAEAA
                     AGDSRTRMVALTANAQTEDRQSILNAGLDGLLLKPLDRERLREILDTAGPGTSPLAA"
     gene            1935..2798
                     /locus_tag="VFL49_06115"
     CDS             1935..2798
                     /locus_tag="VFL49_06115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HET7911584.1"
                     /translation="MSSLQAYGGLRAWAMRAYRPSRAMGRVGMLEVRLAQTAAEIRQA
                     QKLRYQVFYQEGSAIPSPARFLARRDIDGYDAICDHLLVLDHTGRDRGALKRPAVVGT
                     YRLLRQPFAEEYGGFYTAGEFDISALVARHSRLQFLELGRSCVLSPYRNKRTVELLWH
                     GIHAYILENRCDVMFGCASLDGTDPKRLALPLSFLHHYAGAPEPWRARALPERYVEMN
                     RMSKGAIDPKAALRALPPLIKGYLRLGAYIGEGAVIDHGFVTTDVLIILPVSAIKQRY
                     FEHFALSRRAA"
     gene            complement(2801..3754)
                     /gene="coaA"
                     /locus_tag="VFL49_06120"
     CDS             complement(2801..3754)
                     /gene="coaA"
                     /locus_tag="VFL49_06120"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET7911585.1"
                     /translation="MEQRIEENLSPYRTFSRAEWAALREDTPMTLTPEEVTRLRSLHD
                     RLDMTEVEEIYLPLSRLLSMYVAATQRLFVAQERFLGTEDTKMPYVIGVAGSVAVGKS
                     TTARVLQALLARWPNVPKVDLITTDGFLFPNSILEREGLMEKKGFPESYDLPALLRFL
                     SDVKAGRRPARAPIYSHLVYDVIPNQWIEVDRPDILIVEGLNVLQTGRLPKDGKAIPF
                     VSDFFDFSVYLDADVSVLKSWYVDRFLTLRGTAFRDPKSYFHRYSNLSDEEATETASS
                     IWSRINLVNLHENILPTRQRADLILKKSESHLVEEVSLRKL"
     gene            complement(3768..4088)
                     /locus_tag="VFL49_06125"
     CDS             complement(3768..4088)
                     /locus_tag="VFL49_06125"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET7911586.1"
                     /translation="MSKFTLADLEKRVHERAKASAAESYTRALIEKGIAHCAKKLGEE
                     AIETGLAAVQEDNNRLTAEAADLLYHLLVLLEARGVTLADVEAALEKRTAQGGHEEKS
                     ARAR"
     gene            complement(4149..4925)
                     /gene="hisF"
                     /locus_tag="VFL49_06130"
     CDS             complement(4149..4925)
                     /gene="hisF"
                     /locus_tag="VFL49_06130"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET7911587.1"
                     /translation="MFKVRIIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAMAYDAAGA
                     DELCFLDITASHENRDTIYDVVTRTAEACFMPLTVGGGVRTVDDIRKLLVCGADKVSI
                     NTAAVARRPFVKEAAEKFGDQCIVVAIDAKKVSKTGEKGRWEIFTHGGRNPTGLDAVS
                     YAKEVVALGAGEILLTSMDRDGTKAGYDIALTRAVADAVGVPVIASGGVGTLDHLVEG
                     IRDGHATAVLAASIFHFGEHTVRQAKGYMAKAGLPIRMDP"
     gene            complement(4925..>5245)
                     /locus_tag="VFL49_06135"
     CDS             complement(4925..>5245)
                     /locus_tag="VFL49_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET7911588.1"
                     /translation="WSETSELSALDIGRRFEDVGVSAIVYTDIARDGMLQGLNLDATI
                     ALADAISIPVIASGGLASIEDVRALLAPRAEKLAGAIVGRALYDGRLDAADALGLIRA
                     ARAT"
BASE COUNT         1007 a         1597 c         1575 g         1066 t
ORIGIN      
        1 ggttgaatct gccaacggtg cacgttggat ctcctggcgg gaggttgccg tacgcagcga
       61 cggcggtacc gaagttcaga gcgtcggacg agacgtgaca agccgtgtcg aagctgaaca
      121 ggccctcaca gttgcgcgcg atcaggccga ggccgcgagc cgtaccaaat cgcgcttcct
      181 cgccacggtc agtcatgaga ttcgcactcc gctcaacggc atgcttggca tggccgattt
      241 gctgctcgac acgccgctca cgagtgaaca gctgacgtat acgaaggctg cgaaagcttc
      301 gggcgagaca ttgctggcat tgatcgagga gattctcgat ttctcgaaaa ttgaagccgg
      361 caggctcgat gtggaattgc gcccgttcgc actcgccgcc atgatcgagg aatcagttga
      421 gctcatggcc ccgcgtgctc aggcaaaagg aatcgaaatc gtctcctata ttgacgagcg
      481 ggttccggcg aatgtagtcg gcgatgctgc ccgtctgcgt caggttttgc taaacctgat
      541 cggcaatgcc gtgaagttta ccgaaaaggg cggcgtcgga gtcgttgtcg agccgggaga
      601 gtacgagaac gagattcgtt ttgaggttcg cgatacgggc attggaataa agcaagacga
      661 ccagcagcgc atttttgatg actttgagca ggccgatggt tcctcgacgc gaaaattcgg
      721 aggaaccggc ctgggacttg caatctcaaa gcgtattctc gagcgcatga atgggcgcat
      781 cgatgtcaca agccaggtcg gcgtcggttc gatcttcggc atcagcctgc ggttgccgcc
      841 ggcagacacg caggaagcgc ccttcgtcgc ccccgaactc aatcgcctgg cggttatgat
      901 tgtcgcctcg gcagaaatcg aagcgtcatt gcttgcacgc cggctcgggc gatggggcgc
      961 cagcacctgc gccgtgatcg atgagacgat tgccgcggca ctgctgccgg agcggccgtg
     1021 ggatgcgttg cttgttgact atccgcttgc gcgcgcgatg gcggagaacc gcgatctcaa
     1081 taggctgcgt atagcccggc gaatcgtaat gatccgaccg agcgagcggc acgagctgcc
     1141 ggcgctgaaa aaagccggct tcaccggtta cctggtcaag ccggtgcgcg ctgcatcgct
     1201 ggcctcgcgc cttactgcca gcgacatatt cgaatattct gcaaatgacg ccgccaacga
     1261 aacagccgaa gccttgtctc cctccgtcaa gggcctgtcg atcctggtcg cggaggacaa
     1321 cgagatcaat gcattgctgg cacgcgcgct tctcacacga ttgggccatc gtccgaccat
     1381 cgcggcaacc ggcgagatgg cggttgagtt ctggctggca gcgcgtgccg ctggaagccg
     1441 ctacgatctt gtgctggtgg acctgcagat gccgggtctc gatggcttgg aggccgcacg
     1501 ccggatccgc gcagctgaag ccgcagcggg cgactctcgc actcgcatgg tcgcgttgac
     1561 tgccaacgcg caaaccgagg atcgccaatc aatcctcaat gcgggcctcg acgggctgct
     1621 gctcaagccc ctcgatcgtg agcggctgcg tgaaatcctc gataccgccg gccccggtac
     1681 cagcccgctt gcggcgtgac accagtttcg ccacattgtc ataattccgt gtcgggcccg
     1741 gggtgtagag gtatcaccag ccacgagcat gattcggttg cccggatttc acggtcaaca
     1801 tcagtgatgc ctcgccgagc ggacttaaag acaggaccct gatgcgcctg gccatccgca
     1861 ctaattttca cccgccgcgc agtttattcg agcggttgcg cgtcacctcg accggccccg
     1921 gcgcgccggg attcatgtcg tcactgcagg cttacggcgg cttgcgcgcg tgggctatgc
     1981 gcgcttatcg gccttcacgg gcaatgggcc gggtcggcat gctggaagtg cggctcgctc
     2041 agaccgccgc ggagatccgg caggcgcaaa agcttcgtta tcaggttttc tatcaggagg
     2101 gctccgccat tcccagtccg gcccgctttc tcgcgcggcg ggacatcgat ggctacgacg
     2161 cgatatgtga ccacctgctc gtgctcgatc ataccgggcg tgatcgcggc gcgttgaaac
     2221 ggccagccgt ggtaggcacc taccggctct tgcgtcagcc gtttgccgaa gagtatggcg
     2281 gattttatac cgccggcgag tttgacatca gcgctcttgt cgcgcggcat agccgtctgc
     2341 aattcctcga gctcggccgt tcctgcgtgc tttcgcccta ccgcaacaag cgcacagtcg
     2401 aacttctctg gcacggcatc cacgcgtaca tcttggagaa tcgttgcgat gtgatgttcg
     2461 gatgcgcaag tctcgatggc actgatccga aacggctggc gctcccgctg tcgtttctcc
     2521 atcactacgc cggtgcgcca gaaccatggc gcgcgagggc cttgcccgag cgttatgtcg
     2581 agatgaaccg gatgtcgaaa ggggcgatcg acccgaaggc ggcgctgcgc gcgctccctc
     2641 cgttgatcaa aggctacttg cggcttggcg cctatatcgg cgagggcgcg gtgattgacc
     2701 acggattcgt caccacggac gttctgatca ttctgcccgt gtcggcgatc aagcaacgct
     2761 acttcgagca ttttgctctt agccgccgcg cggcctgatc tcaaagcttc cgtagcgaaa
     2821 cctcttccac gaggtggctc tcgcttttct ttagaatgag atcggcgcgt tgccgcgtcg
     2881 gcagaatatt ttcatgcaga ttgacgagat tgatgcgcga ccagatcgat gatgctgttt
     2941 cggtcgcctc ctcgtccgac agattggaat agcggtggaa atacgatttc ggatcacgaa
     3001 acgccgtgcc gcgcagcgtc aggaacctgt cgacatacca ggacttcagc actgatacgt
     3061 cggcgtcgag atagacggaa aaatcgaaaa agtccgagac gaacggaatg gccttgccgt
     3121 ctttgggcag ccgtccggtc tgcagcacat tgagaccctc tacgatgaga atgtcgggcc
     3181 gatcgacctc gatccactgg ttgggaataa catcgtagac gaggtgtgaa tatatgggcg
     3241 cgcgtgccgg gcggcgcccg gctttgacgt ccgacaggaa ccgtagcagc gccggaagat
     3301 catagctttc gggaaagccc ttcttttcca tcaggccttc acgttccagg atagaattcg
     3361 gaaacagaaa tccgtctgtt gtgatgagat cgaccttcgg cacgttcggc cagcgtgcga
     3421 gcagcgcttg cagcacgcgt gccgtcgttg atttgccgac agcgaccgaa cccgcaacgc
     3481 cgatgacata cggcattttc gtgtcttctg tgccgagaaa gcgctcctgt gcgacgaaca
     3541 accgttgcgt cgccgctaca tacatcgaca aaagccgcga gagcggaaga tagatttcct
     3601 ccacttcggt catgtcgagc cggtcgtgca gagagcgtag acgcgtgacc tcctccggag
     3661 tcagcgtcat cggcgtgtcc tcgcggagcg ccgcccattc tgcgcgggag aaggtgcgat
     3721 agggcgagag attttcttcg atgcgctgtt ccatcacaag atcccgatca gcgtgcgcgc
     3781 gcggattttt cctcgtgtcc gccctgcgct gtccgtttct cgagtgccgc ctcgacatcg
     3841 gccaacgtga caccgcgtgc ttcaagcagc accagcagat gataaagaag atcggccgct
     3901 tcggcggtga gccggttgtt gtcctcctga acggcggcca ggccggtttc gatcgcttcc
     3961 tcgccgagct ttttggcgca atgggctatg cctttttcga tcaaggcacg cgtataggac
     4021 tcggcagcgc ttgccttggc gcgctcgtga accctttttt caaggtcggc cagtgtgaac
     4081 ttgctcatcg acagtccttc ggaccgtcct ttatcacctc ttaccgcctg cggggagagg
     4141 tcagcgcgct acggatccat gcgaatcggc aggccagctt tggccatata acccttggct
     4201 tgccgcaccg tgtgctcacc gaaatgaaag attgaagctg cgagtaccgc ggtggcatgg
     4261 ccgtcgcgga tgccttcgac cagatggtcc aaggtgccaa ccccacccga ggcgatgacc
     4321 ggaaccccga cggcatcggc caccgcgcgc gtgagcgcaa tatcatagcc ggctttggtg
     4381 ccgtcgcggt ccattgacgt cagcaggatc tcgcctgcgc ccagcgccac gacttccttg
     4441 gcataactga cagcgtcgag gccggtcgga ttgcgcccgc cgtgcgtgaa gatttcccag
     4501 cggcctttct caccggtctt tgaaaccttt ttcgcgtcga tcgcgaccac gatgcattga
     4561 tctccgaatt tctcggctgc ctccttgacg aaggggcggc gcgccaccgc tgcagtattg
     4621 atcgatactt tgtccgcacc gcagacgagc agcttgcgga tgtcgtcgac cgtgcgcacg
     4681 ccaccgccga cagtcaacgg catgaagcaa gcctcggctg tgcgcgtcac cacgtcgtag
     4741 attgtgtcac ggttttcgtg actcgcggtg atatcgagaa aacagagttc atcggcgccg
     4801 gcggcgtcat atgccattgc cgcttcgacc gggtcgcccg catcgcgcaa atcgacgaaa
     4861 ttcacgccct tgacgacccg gccgtctttc acgtcgagac agggaatgat gcgaaccttg
     4921 aacatcaggt cgcccttgct gcacgaatga ggccgagcgc gtccgcggca tccagacgcc
     4981 cgtcatagag cgcccggcca acgatcgccc ccgccagctt ttccgcacgc ggagcaagca
     5041 gcgccctgac gtcctcgatc gaggcgagac cgcccgatgc gatcaccgga atggaaatcg
     5101 catccgcgag cgcaatcgtg gcgtcgaggt ttagtccctg caacatgcca tcgcgcgcga
     5161 tatcggtata aacgatcgcc gaaactccga cgtcttcaaa ccgacgcccg atatcgagcg
     5221 ccgacaattc ggacgtctcg gacca
//
LOCUS       DASSRL010000296         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_655557, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000296 DASSRL010000000
VERSION     DASSRL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655557"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            533..886
                     /locus_tag="VFL49_06140"
     CDS             533..886
                     /locus_tag="VFL49_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911589.1"
                     /translation="MRRMIVSCVAIAITSLAGIFVAATAEAMTTSVPLAMAGILESGN
                     SIQKAEYVCRRVRRCGPYGCGWRRQCWEAGPGYYGGGYGYYAPRRGYYGRGGAYPCGP
                     GWSLQDGVCKPYRGY"
     gene            complement(984..1289)
                     /locus_tag="VFL49_06145"
     CDS             complement(984..1289)
                     /locus_tag="VFL49_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911590.1"
                     /translation="MVSLGIMIAGGAHAADNKTSWRYNAHGSELPFPRSERAQAVWAS
                     ERCWKDCESYCAWGMAGCLQQNAQGLCLKLTDKCDRHCQRACRTTGGPYLPIEFPWE"
     gene            1469..2293
                     /locus_tag="VFL49_06150"
     CDS             1469..2293
                     /locus_tag="VFL49_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HET7911591.1"
                     /translation="MRSVAEQQNDRDLIPAQKFGTAGITPMARPSLVTRFFMRIVAAV
                     ETLNVALSKFGNPPIYDDAVFPWSRLIEKEWRAIRAELDRILIRKDELPGFHELAADV
                     STISQDRGWKTFMLAGYGFRSDNNIGQCPETWRVCQNIPGLITVMFSILEPGKHLPPH
                     RGPYNGVLRLHLGLIVPEPRDQLGIRVENGVYRWREGEAVVFDDAYEHEASNYTSQTR
                     VVLFVDFRKPLRFPANVINWLLLHLAVFTPFIREGMDNQKAWEQRFYEEAQALRNR"
     gene            2351..3694
                     /gene="rimO"
                     /locus_tag="VFL49_06155"
     CDS             2351..3694
                     /gene="rimO"
                     /locus_tag="VFL49_06155"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7911592.1"
                     /translation="MANPEPKSDAAAPKVSFVSLGCPKALVDSERIVTRLRAEGYELS
                     REHKGSDLVIVNTCGFLDSAKAESLAAIGNALKENGKVIVTGCMGAEPDSISSAYPDV
                     LAITGPQQYETVVDAVHKALPPKHDPFLDLVPPEGIKLTPRHYAYLKISEGCNNRCTF
                     CIIPRLRGDLVSRPAADVLREAEKLVKAGVKELLVISQDTSAYGLDIRYASSKWRDKD
                     VRAKFIDLAAALGELGVWVRLHYVYPYPHVDDVIPLMAERKVLPYLDIPFQHASPEVL
                     KRMKRPAAQEKTLERIEQWRRECPDLTLRSTFIVGFPGETDADFDMLLEWLEQASLDR
                     VGCFKYEPVRGAAANDLGEPVPDQVKEQRWHRFMQAQQKISARRLKRKLGTRQKVIID
                     EVGPTVTKGRSMADAPEIDGAVYAVSRRPLRVGEIATVKVERVDEYDLHGTVVGF"
     gene            complement(3700..>4062)
                     /locus_tag="VFL49_06160"
     CDS             complement(3700..>4062)
                     /locus_tag="VFL49_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7911593.1"
                     /translation="VADATGWASLVNAGQLRLLVLWTASRSKNWPDAPTLREAGIDMV
                     SNSPFGIAGPKGMDPAVVRVIHDAFKRGAEDPVYLDATAKLDQEPAYMSTDDYRRYAA
                     AQLVEQKKLIEDLGLRQD"
BASE COUNT          787 a         1282 c         1209 g          784 t
ORIGIN      
        1 aagcgtaggc gttgctaccg atcagtactg caatcgcgca caacgcgaat cgaacgatat
       61 taattgtcca atgggtcttc ttcggaggac acatggcaca aacctccgcc accacaagaa
      121 cacgctatcc gtgcaggccc acgcccgtaa acgcgccgac ctcatcacct tcagacacgg
      181 ttaacaggcg aaatcgggtc gttttggcgc cgggttaccc aattttaaag ccaaaccggc
      241 agagttccga atggcccgcc tgggtgttca cgccacacgg ggactcccac accgggggac
      301 ggggccgggg gccgcgcgtg tggcagcgcg gcccttcccg cttgagaaat tggactcgcg
      361 acccctgcct acaaggcaga acaagcgcgg tgagccatcg aacgaaatcg gggacccgga
      421 gttatttgga tcagtctcgg gcaagatccg aacagtgctc gcgtgtggtg ttcagccatt
      481 gttcagcgtt tcagcaacac gaattctctg aataaagcca aggagagcaa caatgcgaag
      541 aatgattgtg tcctgcgtgg caatcgccat cacctcgctg gcgggaattt tcgtcgccgc
      601 caccgccgag gcaatgacga catccgttcc actggcgatg gcgggaatcc ttgaaagcgg
      661 aaattcaatt cagaaggctg agtacgtctg ccgtcgcgta cgccgctgtg ggccgtacgg
      721 ttgcgggtgg cggcgccaat gctgggaggc gggtccgggc tactatggtg gcggctacgg
      781 ctattatgcg ccccgtcgcg gctactatgg gcgcggcggt gcttatccgt gtggtccggg
      841 ctggagccta caggacggcg tctgcaagcc ttaccgaggt tactgatacg aggagggccg
      901 ccgctcgcgg cctcctgctc cctgccatcc gggacgccca atcgagttga ccgcgacggt
      961 caccgtgatc aagtggactc gcttcactcc cacggaaact caatcggcaa atacggtccg
     1021 cctgtcgtcc ggcacgcccg ctgacaatgc cgatcgcatt tgtctgtgag cttcagacag
     1081 agcccctgcg cgttctgctg caggcagccg gccattcccc acgcgcaata ggattcgcaa
     1141 tccttccagc agcgctccga agcccagacc gcttgagcgc gctcgctgcg cggaaacggg
     1201 agctcggaac cgtgcgcgtt atagcgccac gacgtcttgt tgtcggcagc atgcgccccg
     1261 cctgcaatca tgattccgag cgagaccaag ccgacaaaaa tccgcaatcg catcatggcg
     1321 agtcacccat ctggcgtcac aatacctccg ccggcgcata gcgccaacgc gcctgaagca
     1381 tgcttgcgga gcttccgatg ccggtcttcc cttggtattg tggcagtact ccgaaaactc
     1441 aagtgggcgt gcgcggagga cgaccgtatt gcgtagcgtc gcagagcaac aaaacgatcg
     1501 cgacctcatc cctgcccaaa aattcggaac ggccggcatc acgccgatgg cgcggccgag
     1561 cctggtgacg cgcttcttca tgcgcatcgt tgctgcggtg gaaacactca acgtcgccct
     1621 gagcaagttc ggcaatccgc cgatctacga cgatgcggtc tttccgtggt ccaggttgat
     1681 cgagaaggaa tggcgagcga tccgcgccga actcgaccgc attctcatcc gcaaggacga
     1741 attgcccggt tttcatgagc ttgcggcgga tgtgtcgacc atcagccagg accgcggctg
     1801 gaaaaccttt atgctcgccg gttacgggtt ccgttcggac aacaatatcg ggcagtgtcc
     1861 ggagacctgg cgtgtctgcc agaatatccc cggtctgatc accgtgatgt tttcaattct
     1921 tgagcccggc aagcatttgc cgccgcatcg tggtccctac aatggcgtgc tccgcctgca
     1981 ccttggactg atcgtgccgg agccgcgcga tcagcttggc atccgggtcg agaacggtgt
     2041 ctatcgctgg cgcgagggag aggccgtcgt atttgatgac gcctatgaac acgaggcatc
     2101 gaactacaca tcgcagacgc gagtggttct tttcgttgat ttccgcaaac ctctgcgttt
     2161 ccccgccaac gtgatcaact ggctgttgct gcatcttgcc gtctttacgc ccttcatccg
     2221 cgagggcatg gacaaccaga aggcctggga gcagaggttt tacgaagagg cgcaggcact
     2281 gcgcaatcgc tagccgccgg cgactttccg ggttgctatt ccatccgatc gccgttagct
     2341 ccttgccgcc atggccaatc ccgagccgaa atccgacgcc gctgcgccga aggtgagctt
     2401 tgtctctctc ggctgcccga aggcgctcgt agacagcgag cgcatcgtca cccgcctgcg
     2461 cgcggagggc tacgagcttt cgcgcgagca caagggttcg gatctcgtca tcgtcaacac
     2521 ctgcggcttc ctcgacagcg ccaaggcgga atcgcttgcc gcgatcggca acgcgctcaa
     2581 ggagaacggc aaggtcatcg tcaccggttg catgggtgcg gagccggaca gcatttcgtc
     2641 ggcctatccc gacgttctgg cgatcaccgg tccgcagcaa tacgaaacgg tcgtcgacgc
     2701 agttcacaag gcattgccgc cgaagcacga tccgtttctt gacctcgtgc ctccggaagg
     2761 catcaagctc acgccacggc actatgctta tctgaagatt tcggaaggct gcaataaccg
     2821 ctgcaccttc tgcattattc cgcgcctgcg cggcgacctt gtctcgcgtc cggcggccga
     2881 cgtcctgcgc gaggccgaga aactggtgaa agcgggcgtg aaggagttgc tggtcatctc
     2941 acaagataca tccgcctacg ggctcgacat caggtacgca tcgagcaaat ggcgcgacaa
     3001 ggacgtgcgc gccaaattta tcgacctcgc ggcggcgctc ggcgaactcg gcgtctgggt
     3061 acgtctgcac tatgtctatc cctatccgca tgtggacgat gtcattccgc tgatggccga
     3121 gcggaaggtt ctcccctatc tcgacatccc ctttcagcac gcttcgcccg aggtcttgaa
     3181 acgcatgaaa cggcccgccg cccaggaaaa gacgcttgaa cgcatcgagc agtggcggcg
     3241 ggagtgcccc gatctgacgc tccgctcgac cttcatcgtc ggctttcccg gcgagaccga
     3301 tgccgatttc gacatgctgc tggagtggct ggagcaggca tcgctcgatc gggtcggctg
     3361 cttcaagtac gagcccgtgc gcggcgcggc tgcgaatgat ctcggcgaac ccgtccccga
     3421 ccaggtgaag gagcaacgct ggcatcgctt catgcaggcg cagcagaaaa tttccgcacg
     3481 ccgtctcaag cgcaagctcg gcacccggca aaaggtcatt atcgacgagg tcgggccgac
     3541 ggtgaccaaa gggcgctcga tggccgacgc gcccgagatc gacggcgcgg tttatgccgt
     3601 cagtcgccgg cccctccgcg tcggcgagat cgccacggtc aaggtcgagc gcgtcgacga
     3661 atacgacctg cacgggaccg tggtgggctt ttaaaggcct cagtcctgcc tgagtccgag
     3721 gtcctcgatc agcttctttt gctcgacgag ttgcgcggcg gcatagcggc ggtagtcgtc
     3781 ggtgctcata taggccggct cctgatccag cttcgccgtc gcatcgagat agaccgggtc
     3841 ctccgcaccc cttttgaacg cgtcatggat gaccctgacc accgccggat ccatgccttt
     3901 tggtccggct atgccgaacg gagaattcga caccatgtcg attcccgctt cgcgcaatgt
     3961 cggcgcatcg ggccagttct tgctgcgtga cgcagtccac aggaccagca gccgcagttg
     4021 cccggcattc accagtgacg cccagccggt ggcgtcggcg ac
//
LOCUS       DASSRL010000297         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_743064, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000297 DASSRL010000000
VERSION     DASSRL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743064"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..501)
                     /locus_tag="VFL49_06165"
     CDS             complement(106..501)
                     /locus_tag="VFL49_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911594.1"
                     /translation="MTPRTQLKLFTAAAALALIGFGAGVAAHAEEPLNNLGPVGPHEP
                     IVIAAGNQRVLAFYEPERGGCAVSAVTWKEAAPDAPFRVRISLKPGQMFHLDGAERQS
                     VGLLCGADASTLAAVAPAELILTGATAKD"
     gene            785..1474
                     /locus_tag="VFL49_06170"
     CDS             785..1474
                     /locus_tag="VFL49_06170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7911595.1"
                     /translation="MLQEWNKAHLINHLAEVHGYRHYLEFCTFSTGLRYAEIDRTKFR
                     TCARLMYGCPDDFEDGLPIDYRSNTLDIGESLKKISSEDRRFDIALVDSWHEYETSWR
                     DLVAAFLLIREGGTLVVHDCLPPRSEIAVPKFIRGEWCGVGYQAYVDFISQRHDLAAY
                     TVDTDYGCGVIRKLAEPSPESATSAGADLLAYWRRKRDDPMEAFAFLQANKQVLLNLI
                     TTDEFFARERS"
     gene            complement(1437..>2011)
                     /locus_tag="VFL49_06175"
     CDS             complement(1437..>2011)
                     /locus_tag="VFL49_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911596.1"
                     /translation="YGLVWADIILAFGIARIREVPSFCRDADCAQFFTEHAKSFWGFW
                     RMGDVARTYLLDRRNLCNTVTWGEQTRGAGFISIDRNAGSVDVEVCLSDREADDGASP
                     LEEWVGIVKDAKDDINKEVLLRLNLAESALRDLDIDAFKSGKPLGTTDIELEVRRSAI
                     QFAPDDIEELAAQSRIPAVSFVPWRKIRRW"
BASE COUNT          411 a          596 c          607 g          397 t
ORIGIN      
        1 agcgcaggct gtggaaaacg aaatgggtgg agcgagctgc ctcggtccac ccatgtatct
       61 ctctaaatgc tcgcctcgcc ggatgtcacc cggcactgac aatacctagt cctttgccgt
      121 cgcgccggtg aggatcagct cggctggagc aacggcggca agcgtcgaag cgtcggcgcc
      181 gcaaagcagg ccgaccgact ggcgctcggc gccatcgaga tggaacatct gacctggctt
      241 caggctgatg cggacacgga aaggcgcatc cggtgcggcc tccttccaag tgaccgcgct
      301 caccgcgcat ccgccacgct caggctcata gaaggcgagc acccgctgat tgccggcggc
      361 gatgacgatc ggctcatgcg ggccgactgg gccgagattg ttcagcggct cctcggcgtg
      421 cgctgctacg cccgcgccga aaccgataag ggcgagggct gcggcggcgg tgaaaagctt
      481 cagttgggta cgtggggtca ttattggcct cctattgaag ttagatgcgc gcgcaaacga
      541 atcacgtgct gcactgcaaa ggtttttatt gcggtgcagt gacagaacta tgttgcagtg
      601 caagacaaac tgacgcgggg ccggctgccg caggaatttt ccgaaggagc aatttggatt
      661 tcagatgatg gcggcgtgag agaacgcgtc gtgtgcacga tgcctcgtcg atgcaatttt
      721 tttgtgcagg gcggatgtgt cggcggcgat tatcatgccc tcatagcttg gtgggggcct
      781 gccgttgctt caagagtgga acaaggcgca cctcatcaat catttggccg aggtgcacgg
      841 ctatcgtcac tatctcgaat tttgcacgtt ttcgaccggg ctgcgctacg cagagatcga
      901 tcggacgaaa tttaggacct gtgcccggct gatgtatggt tgcccggacg atttcgaaga
      961 cggcctgccg atcgactatc gcagcaatac tctcgacatc ggcgaaagcc tcaagaagat
     1021 cagcagcgag gaccgccgct tcgacatcgc gctggtcgat tcatggcacg aatacgaaac
     1081 gtcatggcgc gatctcgtgg cggccttcct gctcattcga gaaggcggca cgctcgtcgt
     1141 gcatgactgc ctgccgccaa ggtccgagat cgccgtgccc aaattcatcc ggggagaatg
     1201 gtgcggcgtc ggctatcaag cctatgtcga tttcatcagc caacggcacg acctcgcggc
     1261 ttacacggtc gacaccgact atggttgcgg cgtgatccgc aagctcgccg agccatctcc
     1321 tgagagcgcg acttctgccg gcgctgacct tctcgcatac tggcgccgca agcgcgacga
     1381 ccccatggaa gccttcgcgt tcttgcaggc caacaagcag gttctactga acctgattac
     1441 caccgacgaa tttttcgcca gggaacgaag ctaacggcag gaatgcgcga ctgtgctgca
     1501 agctcctcaa tatcatctgg tgcgaattga atagcgctcc tgcgcacctc aagctcgatg
     1561 tcggtcgtgc caagtggctt gccagactta aaggcgtcga tgtcaaggtc gcgaagcgca
     1621 ctctcggcaa ggttgaggcg caacaacacc tccttattga tgtcgtcttt ggcgtccttg
     1681 acgatcccaa cccactcttc gagtggtgaa gcgccatcgt cggcctcccg gtcggagagg
     1741 cagacctcga cgtcaacgct gccggcattg cgatcgatcg agatgaaccc ggcgccgcgc
     1801 gtttgctccc cccacgtcac cgtgttgcac aggtttcggc ggtcaagcag gtaggtgcgc
     1861 gccacatcgc ccatgcgcca aaagccccaa aagctcttgg cgtgctcggt gaagaattgc
     1921 gcgcaatcgg cgtcgcgaca gaagctcggc acctcgcgga tcctggcaat gccgaacgct
     1981 agaatgatgt cagcccagac aaggccatag g
//
LOCUS       DASSRL010000298         6739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_95804, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000298 DASSRL010000000
VERSION     DASSRL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95804"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFL49_06180"
     CDS             complement(<1..354)
                     /locus_tag="VFL49_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7911597.1"
                     /translation="MTVQVTRLPSGVSVVTDRMAHLETASLGIWIASGSRNEKPDEHG
                     ISHLLEHMAFKGTNRRTARQIAEAIEAVGGDLNAATSIESTGYFARVLKADVPLAIDV
                     LSDILSEPTFDAEELK"
     gene            complement(351..1766)
                     /gene="thrC"
                     /locus_tag="VFL49_06185"
     CDS             complement(351..1766)
                     /gene="thrC"
                     /locus_tag="VFL49_06185"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7911598.1"
                     /translation="MRYVSTRGEAAPLDFVEVMLAGLARDGGLYVPESWPALDSEVVA
                     GFAGKPYPEVAVDVIRPFIGGSIADADLARMVREAYGGFRHPATAPLVQLDASLFVLE
                     LFHGPTLAFKDLAMQLVARLMDHTLHQRGERRTIVVATSGDTGGAAAEAFRGRGQVDV
                     VVLFPHGRISDVQRRMMTTVPDANVHAIAIEGTFDDCQALVKAMFNHHAFRDRVRLSG
                     VNSINWARIVAQAVYYFTAAVALGAPHRKIAFTVPTGNFGDVYAGYVAARMGLPVDRL
                     VVATNANDILARTFATGAYEIRDVIATMSPSMDIQVSSNFERLLFEASGRDAASVRAL
                     MGSLAQARRFVVSAQALKELRALFSANRADEQECAAEIRAWMREANYCIDPHTAIALA
                     VAEKETRDASVPMVVLSTAHPAKFPAAVKAACGIEPPLPDWLADLQKRKERVTVLPAD
                     QAAVERFVASASRAAREGAAA"
     gene            complement(1851..2603)
                     /locus_tag="VFL49_06190"
     CDS             complement(1851..2603)
                     /locus_tag="VFL49_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET7911599.1"
                     /translation="MKKALRKRRATMLDATMFALAGVAILCGLGVWQLERKVWKENLI
                     ATLNARLAAAPGDLPPRVSWRILKENEAEFRRVTFPAEFLDGEEALVYTAGSPLRPDV
                     KGPGYWVFTPAQLAGGSIVLVNRGFVPADRKDPATRAEGAPHGSVDIVGVIRWPETRG
                     AFTPPDDPKDNVWYLRDSDAIAASKKWLTAAPFYIDQESPVPPGGMPKPGKLEVHLPD
                     NHLQYAITWFGLALALAGVYVAWLARRLFGRY"
     gene            complement(2600..2986)
                     /locus_tag="VFL49_06195"
     CDS             complement(2600..2986)
                     /locus_tag="VFL49_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HET7911600.1"
                     /translation="MTDQPQPESLPIGRGLRARCPRCGEGHLFQGFLALRPACEHCKL
                     DYGFADAGDGPAVFVILIGGGIVVFAALITEVLYQPPYWLHALLWLPLILIVTLVPLR
                     PLKGLLIALQYHHKAAEGRLERKGDA"
     gene            complement(3060..3905)
                     /locus_tag="VFL49_06200"
     CDS             complement(3060..3905)
                     /locus_tag="VFL49_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7911601.1"
                     /translation="MADAHAKHHDYHLVDPSPWPAVGSVSAFIMAVGGISWMHHMYPA
                     APLVFGVGVVGVLYTFIAWWRDVIREAQFEGFHTRVVQISHRYGMILFIASEVMFFVA
                     WFWAYFNTALFPGEPHEVGRLAVTGGVWPPKGIETFDPWHLPLLNTLILLTSGTTVTW
                     AHHALLEDDRQGLKWGLILTILLGLTFTCVQAFEYSHAHFHFSGNIYGATFFMATGFH
                     GAHVIIGSIFLIVCLLRAMAGHFTPKQHLGFEFAAWYWHFVDVVWLFLFACIYVWGAG
                     APHPE"
     gene            complement(3920..4525)
                     /locus_tag="VFL49_06205"
     CDS             complement(3920..4525)
                     /locus_tag="VFL49_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7911602.1"
                     /translation="MADPGHSSDRKPLRRDALVAACCGAVVVSMVGAAYAAVPFYNWF
                     CRTTGFGGTTQVSEKAPDHVLGRTITIRFDSNVTPGLPWKFVPERNEIKVRIGEVATV
                     NYKVVNEAAREIVGQASYNVSPPTVGAYFSKINCFCFTEQRMKPGETREMTVVFYVDP
                     AIVKDRDQDRLNTITLSYTFYRLRDQDRPVAETPVADSKKL"
     gene            complement(4534..4698)
                     /locus_tag="VFL49_06210"
     CDS             complement(4534..4698)
                     /locus_tag="VFL49_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoxF protein"
                     /protein_id="HET7911603.1"
                     /translation="MSEPHDDDGIVLTEAQKRARRSRSIAIALALAALVILFYVMTLV
                     KGPGVLIRPI"
     gene            complement(4713..5666)
                     /locus_tag="VFL49_06215"
     CDS             complement(4713..5666)
                     /locus_tag="VFL49_06215"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET7911604.1"
                     /translation="MSLVSEGADFRGSLSSGLAEPSLATVGDYFTLMKPRVMSLVVFT
                     ALVGLAVAPGSLHPLTGFTALLCIAVGAGAAGALNMWYDADVDAVMTRTSRRPVPMGR
                     VQPGEALAFGLTLAGFSVATLGLLVNWLAAGLLAFTIFFYVAVYTVWLKRSTPQNIVI
                     GGAAGAFPPMIGWAATTGSLSLEPVLLFLIIFFWTPPHFWALALYRTDDYARAGIPML
                     PVVSGDASARRQILFYTLILVPLAIAPWPLGYTGAIYGVTAVISGAVMLLLGWQVLHE
                     RAPTERASRNLFAFSVLYLFLLFAVLLVDRGWGGLIARLSA"
     gene            complement(5684..>6739)
                     /locus_tag="VFL49_06220"
     CDS             complement(5684..>6739)
                     /locus_tag="VFL49_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7911605.1"
                     /translation="NFITTIFNMRAPGMTLHRMPLFVWSILVTAFLLLLSLPVLAGAI
                     TMLLTDRTFATTFFSPEGGGDPLLYQHLFWFFGHPEVYIMILPGFGIISHVVSTFSRK
                     PVFGYLGMAYAMVAIGFVGFVVWAHHMYMAGLDVDTRAYFVAATMIISVPTGIKIFSW
                     IATMWGGSIEFKTPMLWALGFIFLFTIGGVTGVVLANAGVDRVLQDTYYVVAHFHYEL
                     SLGAVFAIFGGWYYWFPKMTGYMYNETIGKLHFWVTFIGVNLVFFPQHFLGLAGMPRR
                     YVDYPDAFHGWNMVSSYGSYISGFGVVIFFFGMIEAFAKKRVAGNNPWGVGATTLEWT
                     LSSPPPFHQFETLPRIK"
BASE COUNT         1433 a         2150 c         2070 g         1086 t
ORIGIN      
        1 cttgagctct tccgcgtcga aggttggctc ggaaagaatg tcggagagca catcgattgc
       61 gagcggcaca tccgccttga ggacgcgcgc gaaatagccg gtcgattcga tgctcgtcgc
      121 cgcgttgagg tcgccgccaa cggcctcgat cgcttcggca atctgccgcg cggttcgccg
      181 attcgtgccc ttgaacgcca tatgctcgag cagatgtgaa atcccgtgct cgtcgggctt
      241 ctcattgcgg ctgcccgacg caatccatat gccgagcgac gccgtttcga gatgcgccat
      301 ccggtcggtg accaccgaca caccggatgg aagacgagtc acttgcacgg tcatgccgct
      361 gctccttccc gcgcggcacg cgaggccgac gccacaaatc gttcgactgc cgcctggtcc
      421 gccggcaaca ccgtgacgcg ttctttccgc ttctgcagat cggccagcca atcggggagc
      481 ggcggctcga taccgcaagc ggcctttacg gcagccggaa atttcgccgg gtgcgcggtc
      541 gacagcacca ccatcggcac ggacgcgtca cgtgtctcct tttcggcaac cgccagcgcg
      601 atggccgtat gcggatcgat gcaataattt gcttcccgca tccatgcgcg aatttcggca
      661 gcgcactcct gctcgtccgc acgattggcg ctgaacagcg cgcgcagttc cttgagcgcc
      721 tgcgccgaca ccacgaagcg gcgcgcttgt gcaagcgacc ccatcagcgc ccgcaccgaa
      781 gccgcgtcgc ggccgctggc ctcgaacagc aggcgctcga agtttgacga gacctggata
      841 tccatcgacg gcgacatcgt cgcaatcaca tcacggatct cgtaagcgcc cgtagcgaag
      901 gtccgcgcca ggatgtcatt ggcatttgtc gcaaccacaa gccgatcgac cggcagcccc
      961 atacgcgctg cgacatagcc ggcatagacg tcgccgaagt tgccggtcgg caccgtgaag
     1021 gcgattttcc ggtgtggcgc gccaagcgcc acggccgccg tgaagtagta gacggcctgc
     1081 gccacgatgc gcgcccagtt gattgaattg acgcccgaca ggcgcacgcg gtcgcggaac
     1141 gcgtggtgat tgaacatggc cttcaccaac gcctggcagt catcgaaggt gccctcgata
     1201 gcgatcgcat gcacattggc atcgggtacg gtggtcatca tgcgccgttg cacatcggag
     1261 atgcgtccat ggggaaacag caccaccacg tcgacttgcc cgcgtcccct gaacgcttct
     1321 gctgcagcgc cgccggtgtc gccggacgtc gccacgacga tggtcctccg ctcgccgcgc
     1381 tgatgcagcg tgtggtccat caggcgcgcg accagctgca tcgccagatc cttgaacgcg
     1441 agggtcgggc catgaaagag ctcgagcacg aacaggctgg cgtcgagttg cacgagcggc
     1501 gccgtcgccg gatggcggaa tccgccatag gcctcgcgca ccatgcgcgc caggtcggca
     1561 tccgcgatgc tgccgccgat gaaggggcgg ataacgtcca ctgccacttc ggggtagggc
     1621 ttgccggcga agcctgcgac cacctcgctg tcgagcgccg gccaggactc cggaacatag
     1681 agtccaccgt cgcgggccag acccgcgagc atcacctcga caaaatcaag cggggcggcc
     1741 tcgccccggg ttgagacgta acgcattgat ttcgctttat tttggggacc gattgcgccc
     1801 ctgcgctcga ccgcacagta cccgcgggca cctgttttca caagaaaagc tcaatagcgc
     1861 ccaaagaggc ggcgggcgag ccatgccacg tagacgccgg caagcgccag cgccaggccg
     1921 aaccaggtga tggcatattg gagatggttg tcaggtagat gcacctcgag cttgcctggc
     1981 tttggcatcc cgcccggcgg aacaggcgac tcctgatcga tgtagaacgg cgccgcagtc
     2041 agccatttct tcgatgcggc gatcgcatcg gaatcgcgca gataccagac gttgtccttt
     2101 ggatcgtcgg gcggagtgaa cgcgccgcgc gtttccggcc agcggatgac gccgacaatg
     2161 tccacgctgc catgcggcgc gccctctgct cgtgttgccg gatccttgcg gtcggccgga
     2221 acgaacccac ggttgacaag aacaatgctg ccgcccgcaa gctgtgccgg cgtgaagacc
     2281 caatagcccg gtcccttcac atccggacgc agcggcgagc cagccgtgta aaccaatgcc
     2341 tcttcgccgt cgagaaactc ggcgggaaac gtgacgcggc gaaattccgc ctcgttctcc
     2401 ttcagaatcc tccagctgac gcgtggcggc agatcccccg gcgccgctgc gagccgcgca
     2461 ttcagtgtcg cgatcagatt ttccttccag accttgcgct cgagttgcca cacgccgagt
     2521 ccgcacagga tcgccacacc ggcgagcgcg aacatggttg catcgagcat ggtggcgcgg
     2581 cgcttgcgca gcgctttctt cacgcgtcac cttttcgctc aagacggccc tcggcggcct
     2641 tgtgatgata ctgcaaggcg atcaacaggc ctttaagcgg acgcaacggc acaagcgtca
     2701 cgatcaggat caatggcagc cacagcagcg cgtgcagcca atacggcggc tgatagagca
     2761 cctcggtgat cagcgcggcg aaaaccacga tgcctccgcc gatcagaatg acaaacaccg
     2821 caggcccgtc acccgcatca gcgaagccgt agtcgagttt acagtgctca caggccggac
     2881 gcagagccag aaagccttgg aaaagatgac cctccccgca gcgcgggcag cgtgcgcgca
     2941 gaccgcgacc aatcggcaaa ctctccggct gtggttgatc ggtcatggcg ttacaaacac
     3001 atcaaaacaa aggggcggcg tgatgacgcc gccccctttt tcaaaactat ccgcgccgct
     3061 cactccggat gcggcgcacc agcgccccac acatatatgc aggcaaacag gaacagccat
     3121 accacgtcga cgaaatgcca gtaccaggcg gcgaattcga agcccaggtg ctgctttggg
     3181 gtgaagtggc cggccatcgc gcgcagcaga cagacgatca ggaagatcga gccgatgatc
     3241 acgtgcgcgc cgtgaaatcc ggtcgccatg aagaacgtgg cgccgtagat gttgccgctg
     3301 aaatggaagt gcgcgtgtga gtactcaaag gcctgcacgc aggtgaaagt aagtccgagc
     3361 aggatggtga ggatcagacc ccatttcagc ccctgccggt cgtcttccag aagcgcgtga
     3421 tgggcccagg tcactgtggt gccggacgtg agcaggatca gggtattgag cagcggcaag
     3481 tgccagggat cgaaggtctc gatccccttc ggcggccata caccgccggt gacggcaagc
     3541 cgcccgactt catgcggctc gcccggaaac agcgccgtat tgaagtaagc ccagaaccag
     3601 gcgacgaaga acatcacttc agaggcgatg aacaggatca tcccgtagcg gtgcgaaatc
     3661 tgcacgacgc gtgtgtggaa gccctcgaac tgcgcctcgc ggattacgtc gcgccaccag
     3721 gctatgaacg tataaagcac gccaaccacg ccgacgccga ataccagtgg cgcggcgggg
     3781 tacatatggt gcatccagct gattccgccg accgccataa tgaacgcgga taccgaaccg
     3841 accgccggcc atgggcttgg atcgaccaga tggtagtcat gatgcttggc gtgggcgtcg
     3901 gccatcgtcc tcgtctccgt taaagctttt ttgaatccgc aactggcgtc tcagccaccg
     3961 gccgatcctg atcgcgcagc cggtagaaag tgtacgacag tgtgatggtg ttgagcctgt
     4021 cctggtcgcg gtccttgacg atcgcaggat ccacatagaa gacgaccgtc atctcacgag
     4081 tctcgcccgg cttcatgcgc tgttcggtga agcagaagca gttgatcttg ctgaaatagg
     4141 cgcccacggt cggcggcgag acattgtaag aagcctgccc aacaatttcg cgggccgcct
     4201 cgttgacgac cttgtagttg acggtcgcca cctcgccgat ccgcaccttg atctcgttcc
     4261 gctcgggcac gaacttccac ggcagcccag gtgtgacatt ggaatcaaac ctgattgtga
     4321 tggtgcgtcc aagaacgtga tcgggcgctt tttccgaaac ctgggtcgtg ccgccaaatc
     4381 cggtcgtcct gcaaaaccag ttgtaaaagg gcactgcggc ataggccgcc ccgaccatgc
     4441 tcaccacaac ggcaccgcag caggccgcga ccagcgcgtc gcgccgcagc ggctttcgat
     4501 cggaagagtg gccgggatca gccatcgttt cgctcagatc ggccggatca agacgcctgg
     4561 gcccttcacc agcgtcatga cgtagaacaa tatgaccagc gccgcgagcg cgagcgcgat
     4621 cgcgatcgag cgcgaacgcc gcgcgcgctt ctgcgcctcg gtgagcacga ttccgtcatc
     4681 gtcgtgcggc tcgctcatga caactcccgt gctcacgcgg acaaccgggc aatcagcccg
     4741 ccccatccgc gatcaaccag cagcactgca aagagcagaa atagataaag aacggagaag
     4801 gcaaacaggt tgcggcttgc acgttcagtg ggcgcccgct catgtagaac ctgccagccg
     4861 agcagcagca tgaccgcacc gctgataacg gcggtcactc cgtagatcgc gccggtatag
     4921 ccgagcggcc agggcgcgat ggcaagtggc acgaggatca gcgtgtagaa caagatctgc
     4981 cgccgcgcgc tggcatcgcc gctcaccacc ggcagcatcg gaattccggc gcgcgcataa
     5041 tcgtcggtcc tgtagagggc gagcgcccag aaatgcggcg gcgtccagaa gaagatgatc
     5101 aggaacagca gaaccggctc aagcgacagc gagcccgtcg tcgccgccca gccgatcatc
     5161 ggcggaaagg cgccggcggc gccgccgatc acaatgttct gcggcgtcga gcgcttgagc
     5221 cacaccgtgt aaacggcaac gtaaaagaag atcgtgaaag caagcaggcc ggccgcgagc
     5281 cagttcacca gaagaccgag ggtcgcaacc gaaaacccgg cgagcgtgag gccgaaagcc
     5341 agcgcttcgc ccggctgcac gcggcccatc ggcaccggac ggcgcgacgt tcgcgtcatc
     5401 accgcatcga cgtcggcgtc ataccacatg ttgagcgcgc ccgcggcgcc ggccccgacc
     5461 gcgatgcaga gcagcgccgt gaacccggtc agcgggtgca gcgagccggg cgcaaccgca
     5521 agcccaacca gcgccgtgaa caccaccagc gacatcacgc gaggcttcat caatgtgaag
     5581 taatcgccga cggtcgccag gctcggctcg gcaaggcccg acgagagcga cccgcggaag
     5641 tctgcgcctt cgctgacgag agacataagc tgcgccgatt gccctatttg atccgcggca
     5701 gggtttcgaa ctggtgaaac ggcgggggtg acgacagtgt ccactcgagc gtggtcgcgc
     5761 cgacgcccca tggattattg cccgcgaccc gcttcttcgc gaatgcctcg atcatgccga
     5821 agaagaagat gaccacgccg aagccggaaa tgtacgagcc gtaggacgag accatattcc
     5881 agccgtggaa cgcatccgga taatcgacgt agcggcgcgg catgccggca aggccaagga
     5941 agtgctgcgg gaagaagacc aggttcacgc cgatgaaggt gacccagaag tgcagcttgc
     6001 cgatcgtctc gttgtacatg tagccggtca ttttcgggaa ccagtagtac cagccgccga
     6061 aaatcgcgaa cacggcgccg agtgagagct cgtaatggaa gtgcgccacc acgtaatacg
     6121 tgtcctgcag cacgcggtcg acaccggcgt tggcgagaac cactccggtc acgccgccaa
     6181 tcgtgaaaag gaagatgaag ccaagcgccc acagcatcgg cgtcttgaac tcgatcgagc
     6241 cgccccacat cgtcgcgatc caagagaaaa tcttgatgcc cgtgggcacc gagatgatca
     6301 ttgttgcggc aacgaaatag gcgcgggtat cgacgtcgag gccggccatg tacatgtggt
     6361 gcgcccagac gacgaagccg acgaagccga tcgcgaccat cgcgtaggcc atcccgagat
     6421 agccgaacac cggcttgcgc gagaaggtcg agacgacatg gctgatgatc ccgaaccccg
     6481 gcaggatcat gatgtagacc tcggggtgac cgaagaacca gaatagatgc tgatacagga
     6541 gcgggtcgcc accgccctcc ggactgaaga acgtggttgc gaaagtccga tcggtcagca
     6601 gcatcgtgat cgcgccggca agcaccggca gcgacaacag cagcagaaac gcggtgacca
     6661 ggatcgacca tacaaacagc ggcatgcggt gcagcgtcat gcccggggcg cgcatgttga
     6721 agatcgtcgt gatgaaatt
//
LOCUS       DASSRL010000299         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1530456, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000299 DASSRL010000000
VERSION     DASSRL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530456"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VFL49_06225"
     CDS             complement(<1..1608)
                     /locus_tag="VFL49_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7911606.1"
                     /translation="MSLSHSSAHVNRLAQATSPYLLQHKHNPVDWWTWGPEALGEAER
                     TNRPILLSVGYAACHWCHVMAHESFEDEATARVMNELFVNIKVDREERPDIDQIYMNA
                     LHLLGEQGGWPLTMFLTPKAEPVWGGTYFPKESRFGRPAFVDVLREVSRLFREEPSRI
                     EQNRAALLSRLAEKARPAGKVIIGLKELDAAAMQVGNMFDPVNGGLRGAPKFPQPAIL
                     EMLWRAGLRTNDARFFETVEHTLARMSEGGIYDHLGGGFTRYSVDERWLVPHFEKMLY
                     DNAQLLELLSLAWQRSDNPLFARRARETVAWLSREMTTGEGAFSASLDADSEGEEGKF
                     YVWSYEEVIKCLGVADGEYFARHYDVTPGGNFEGHNILNRLAGINCPAEDEVQLARMR
                     VKLLAVRDTRVHPGLDDKVLADWNGLMISALVNSALILDEPDWLAMARRAFDFIARTM
                     TKDDRLGHSWREGKLLFPGLASDFATMIRAALALYEATGERGYLEQALAWQDSFDKHY
                     ADAETGGYYLSADDADDLLLRPHSTQDD"
     gene            complement(1722..2528)
                     /locus_tag="VFL49_06230"
     CDS             complement(1722..2528)
                     /locus_tag="VFL49_06230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7911607.1"
                     /translation="MAPGARLIAVLLAILIACFSQPLAAADNDLARAYRVIASRRFVD
                     LTHSFDSRSPVWSGFGQAKMTPAADPKTQRPYTIKEDGFRTTLYELVGQYGTHVDPPA
                     HFDENGITMDAIPLKQMILPLIVLDATPYLQKDPNHAFSMADLRTWEKRHGRIPRGSF
                     VALRTDMYKDWDASPERFKRAPFPAWSFETIKFLYEQRGVVATGHESLDTDTTDKMES
                     ETYLLQHGHYQIEVMANLDKVPPKGALIVVTWPKVRNGLGFPARAFAILP"
     gene            2696..>2892
                     /locus_tag="VFL49_06235"
     CDS             2696..>2892
                     /locus_tag="VFL49_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007698695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7911608.1"
                     /translation="MSNVARDTIFALSSGRPPAAIAVVRISGPRAGEALKALMGRIPD
                     PRKAALARIRGRGGEIVDQALA"
BASE COUNT          575 a          867 c          871 g          579 t
ORIGIN      
        1 gtcgtcctga gtcgaatgtg gccgcagcag caggtcgtcg gcatcatcgg cagaaaggta
       61 atagccgccg gtctcggcgt cggcgtagtg cttgtcgaaa gaatcctgcc acgcgagagc
      121 ttgctcgaga taaccgcgtt cgccggttgc ctcatagagc gccagcgccg cacgaatcat
      181 ggtcgcgaaa tcggatgcca ggcccggaaa gagcaatttt ccctcgcgcc aggagtggcc
      241 gagccggtcg tctttggtca tcgttcgggc aatgaaatcg aaggcacgcc gggccatcgc
      301 gagccagtcc ggctcgtcaa gaataagcgc cgaattgacc aaagccgaga tcataagacc
      361 gttccaatcg gccaggacct tgtcgtcaag cccgggatgg acgcgcgtgt cgcgcaccgc
      421 aagcagcttg acgcgcatcc gtgccagctg gacttcgtct tcggcggggc agttgatacc
      481 ggcgagacgg ttaaggatgt tatggccttc aaagttgcct cccggtgtaa cgtcgtagtg
      541 gcgtgcgaaa tattcgccgt cggcaacgcc gaggcatttg atcacctcct cgtatgacca
      601 gacgtagaat tttccttctt cgccttccga atcggcgtcg agcgaggccg aaaaagcgcc
      661 ctcgccagtc gtcatctcgc ggctgagcca tgcaacagtt tctcgcgcgc gccgggcaaa
      721 cagaggattg tcactgcgct gccacgccaa cgacagcagc tcaagcagtt gggcattgtc
      781 gtagagcatt ttctcgaagt gcgggacaag ccaacgctca tccacggaat agcgggtaaa
      841 tccgccaccg agatgatcgt agatgccgcc ctcgctcatg cgggcgaggg tgtgctcgac
      901 ggtttcgaaa aagcgcgcat catttgtgcg caggccggcg cgccaaagca tttcgagaat
      961 tgccggttgc ggaaacttcg gagcgccgcg cagaccgccg ttgaccgggt cgaacatgtt
     1021 gccgacctgc atggctgccg catcgagttc ctttaatccg attatcacct tgccggccgg
     1081 ccgggccttc tcggcaagtc gcgacagcag ggccgcccga ttctgctcga tccggctggg
     1141 ctcttcgcgg aacagccgtg acacctcccg caggacatcg acaaaagcgg gtcggccgaa
     1201 gcgcgattct tttggaaaat atgtgccgcc ccagaccggc tccgccttcg gggtcagaaa
     1261 catcgtcaaa ggccagccgc cctgctctcc cagcagatgc aatgcgttca tgtaaatctg
     1321 gtcgatgtct ggacgttctt cccggtcgac cttgatgttg acgaacagct cattcattac
     1381 gcgtgcggtg gcctcatctt cgaaagactc gtgcgccatc acatggcacc agtggcaggc
     1441 cgcatagccg accgacagca ggatcggcct attggtgcgc tcagcctcac ccaatgcttc
     1501 cgggccccag gtccaccaat cgactggatt gtgtttgtgc tgaaggaggt agggtgaggt
     1561 cgcctgagca aggcgattca cgtgggcgct gctgtgagaa agtgacatcg cccgacccta
     1621 gcgtgattgg ccgccagcgg gaaaccacct ctaccggggc gcgtcgactg aaaatagaaa
     1681 cggcgcgatt gatcgaattg ttcctgacgg ccgacaccgc ctcacggtaa aatcgcaaaa
     1741 gcccgtgcgg gaaacccgag tccgttgcgg accttgggcc aggtcacgac gatcaatgcg
     1801 cccttcggcg gcaccttgtc gagattggcc atgacttcga tctggtaatg accatgctgc
     1861 aggaggtagg tctccgactc cattttatcg gtcgtgtcgg tgtcgagtga ctcgtgcccc
     1921 gtcgcgacca cgccgcgctg ctcgtaaagg aacttgattg tttcgaatga ccaggccgga
     1981 aacggcgccc gcttgaatcg ctcagggctg gcgtcccaat ctttgtacat gtccgttcgc
     2041 agcgccacaa aagacccgcg cgggatgcgc ccatgccgct tctcccacgt gcgtaaatcc
     2101 gccatcgaaa acgcgtgatt cggatccttc tgcagatacg gcgttgcatc caatacaatc
     2161 agcggaagga tcatctgttt gagaggaatc gcatccatcg ttattccatt ttcgtcgaaa
     2221 tgcgccggcg gatcgacgtg agtaccgtac tggccgacga gttcgtagag cgttgtgcgg
     2281 aagccatctt ccttgatcgt gtaagggcgc tgggtttttg gatcggctgc gggcgtcatc
     2341 ttcgcctggc cgaagccgga ccacacaggc gaccttgagt cgaagctgtg cgtgagatca
     2401 acgaaacgcc ttgacgcaat cacacggtac gcgcgagcta gatcattatc ggctgcggcc
     2461 aaaggctggc tgaaacacgc gatcagaatc gccagcagga ctgcgatgag acgggcaccg
     2521 ggtgccatgt agcctctccg atcaatcgtg aatttgcacg atcatatcgt gggcggcgga
     2581 atggcacctc agggattttg cgcgcgccgt atgcggtcac ctgaggacga tttgcgatct
     2641 gcgccaacgt gaccgggctt gacccctgcc cgtctcagcg cttccgtgcg ctgaaatgtc
     2701 gaacgttgcg cgcgatacca ttttcgcact gtcctccggg cgtccgccgg ccgccattgc
     2761 cgtcgtgcgg atttcggggc cgcgtgccgg cgaagcgctc aaggcgctca tgggcaggat
     2821 ccccgatccg cgcaaggctg cgcttgcacg catccgtggg cgtggtggcg aaatcgtcga
     2881 tcaagcgctg gc
//
LOCUS       DASSRL010000300         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1023255, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000300 DASSRL010000000
VERSION     DASSRL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023255"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFL49_06240"
     CDS             complement(<1..271)
                     /locus_tag="VFL49_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911609.1"
                     /translation="MQATGIYSDVFLFHSSNPSQTSTSISLNLDAFGTMAVGGPFATS
                     EILLRVNVTGTSVGELRANLDTTGPAHCASSFSGGAGCSGAFFAGR"
     gene            851..>4040
                     /locus_tag="VFL49_06245"
     CDS             851..>4040
                     /locus_tag="VFL49_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7911610.1"
                     /translation="MLHNLIRNREHVVSRNALLADVWQGRVVSDATVSSRINSARSAI
                     GDNGEQQRLIRTVHSKGFRFVGAVVEGTEALAEAAPQQRESVAAGATAAREVSELALA
                     DKPVAELSPVSRFGGGAERRQMTVMLAGATAPSARPDPEELYATMNAHHRRVRDIVGG
                     FGGHVARHAADTLMVYFGYPQAQEDDPERAIRSALAIIDASTPQLQLRIGIATGLVVV
                     GEPGGDGHAQEPSVVGDPPGLAARLHALAEPQTIVVSAGTRRVAGGAFEYRALVSPSV
                     EAWQVLGASAAESRFEARHTAGWSSLVGRAAELELLHSRWRQIVDGEARVVVIAGEAG
                     IGKSHLVHALQQQLADEPHLQIRFFCSPYHRTSALFPVSSNLERAAGFQGDEKVGDRL
                     DKLNELLVPSGARSEQVGLVAELLGLPALGRYRPPDLTPQQRKEKIFEVVLSHILALS
                     RRQPVMMLFEDVHWIDPTSLELLTALIERAQNARVLVLITARSEFPVPWARHAHVSTL
                     SLPRLSRREGAALVGEITGGKVLPDQIFNQILDRADGVPLFLEELTKTVVESGLLRDA
                     DDRYEIAGQLSPTAIPATLHDSLMARLDHVTARAVAQMAACIGRDFDRSLLTAVSGLT
                     EERLREALDELRRTGLIVATGIGANERHSFRHALIRDAAYESLLKGRRAQLHAAIAGA
                     LERSFADVVAAQPEIVAHHFSEAGQPDKAITYWVQAGQRATLRSANAEAFSHLQNGLE
                     LLDQITDARERDRCELLLRTILGAVYVATKGYSAAETVATFKRAADLLQATGDLRLRL
                     AVHNGLLVGYYNLARFDGALDLAQETLRQGEKDGDDAVICVGHRMVAAVCNSVGEFER
                     AAHHARRGWELYRPERHGLAALGLVHDTGLGAKLHLALALCHLGFQNQSKQAAAEALS
                     LAADLHHVNSQAYAWFFAAVLVNFVGRNHSALIEYAPRLRTYAQQHEMPQWAAYGRAF
                     GAVPLVSSGRAGVALDEITGAIGDCERIQNFVFRPAQLTILASAELAVGRADRALAAT
                     ASALEIAEHTREHWLTAETCRIRGHALLSMEDP"
BASE COUNT          731 a         1258 c         1311 g          740 t
ORIGIN      
        1 gcctaccggc aaaaaaagcg ccgctgcagc cggcgccgcc gctgaacgag cttgcgcaat
       61 gggcaggacc cgttgtgtcg aggttggccc tgagctcgcc gaccgacgtg cctgtgacgt
      121 tgacccgcag gaggatctcg gaggtcgcaa acgggccgcc gacagccatc gtgccgaacg
      181 catccaggtt gagcgagatc gacgtgctgg tctgtgaagg gttggaagag tgaaagagga
      241 atacgtcgga gtagattccc gtcgcttgca tgttggtccc ggccgcagaa ccgaacgact
      301 ggaggtcttg ggatccgccg acatggcccg cgctggcgct tgccgcgaca gaaccggtcg
      361 ccgcaaaagt ggggtcgcca agattgctgc agctttggct gttgctgacc ggcgatgaac
      421 cggtattaaa aaaccccgta cagccgccgc cggctccgcc gcccgccacc gtaaaagtga
      481 ccgccaatga tggggagctt ccgagaagcg tcaggaagaa ggccgcgcaa aaaacggggc
      541 ctgcggcagc aaaaagcctg gattgtcgga ccatcacgct ctccctgaac aggggccgcc
      601 acaagctccg ttgatcgcgt ttttgtggca gaaacgccct gtcgttgctt ggagagcagc
      661 agcagaaacc tttgattttg ctttcagaac gacttgattt tgcttttgcc gacccggccg
      721 ctattttgat cgccggtggc ttgggccagg caacttgctg tatcagttcg agaattgttc
      781 cttagacagc gatcgacgag agctgcgtcg cgcgggcagg ctcgttgcgg ttgagccgca
      841 agtattcgac gtgctgcaca acctcatccg caaccgcgaa cacgtggtca gcaggaacgc
      901 cttgcttgcc gatgtctggc agggacgcgt cgtttcggat gcaacggtga gcagccgtat
      961 caattccgcc cgctcggcga tcggcgacaa tggcgagcag cagcgcctga tccgtaccgt
     1021 gcacagcaaa ggcttccgtt ttgtcggagc ggtcgtggaa ggaacggaag ccttggctga
     1081 ggcggcgccg cagcagcggg agagcgtcgc agcgggtgcg acggcggccc gagaagtgtc
     1141 cgagctcgcg ctggcggata aacctgtcgc agaactgtcg ccggtttccc gctttggcgg
     1201 tggcgccgag cgccgtcaga tgacggtgat gcttgcaggc gcaaccgcgc cgtcagcgcg
     1261 tcccgatccg gaagagctgt acgccacgat gaatgcccac catcgccgcg tacgagacat
     1321 cgtcggtggt tttgggggac atgtcgccag gcacgcggcg gacaccctca tggtctactt
     1381 cggatatccg caggcacagg aggatgatcc ggaacgcgcc attcgctcgg ccctcgccat
     1441 aatcgatgcg tccacgcctc aactgcagct gcggatcggc attgccacgg gactcgtcgt
     1501 ggtgggcgaa cccggcggcg acggccatgc gcaagagccc agcgtggtcg gcgatcctcc
     1561 tggtctggct gcgcgactcc acgcgttggc cgagccgcag acaatcgtcg tcagcgccgg
     1621 cacgcgccgg gttgccggcg gcgcctttga gtaccgtgct ctcgtcagcc cctcggtcga
     1681 ggcctggcaa gtgctcgggg caagcgcagc ggagagccga ttcgaggcgc gacatacagc
     1741 cggctggtca tcgctggtcg ggcgcgcggc cgagctcgag ctgctgcaca gccgctggcg
     1801 gcaaatcgtc gacggcgaag cccgcgtcgt ggtgattgcc ggcgaagccg ggatcggcaa
     1861 atcgcacctg gtccacgcat tgcagcaaca gttggccgat gaaccacatt tgcagatccg
     1921 gtttttctgc tcgccctatc atcgcacgag cgcattgttc cccgtcagca gcaacctcga
     1981 gcgggccgcc ggctttcagg gcgatgagaa agtcggcgac cggctggaca agctaaacga
     2041 gttgctggtg ccgtcggggg cacgttccga gcaggttggg ctggttgccg agttgctcgg
     2101 cttgccggcg ctcggccgct accgcccgcc agacctcaca ccgcaacagc gcaaggaaaa
     2161 gatcttcgaa gtggtgctgt cgcacatact ggctttatcg cgacgtcagc cggtgatgat
     2221 gctgttcgag gacgtgcact ggattgaccc gaccagcctc gaactgctga cggccttgat
     2281 cgagcgcgcg caaaacgctc gcgtgctggt gctcatcacc gcccgctccg aattccctgt
     2341 gccctgggcg cgtcatgccc acgtcagcac gttatcgctg ccgcgcttgt cgcgccgcga
     2401 gggagcggcg ctcgtcgggg agatcaccgg tggaaaggtg ctgcctgacc agattttcaa
     2461 tcagatcctt gatcgcgcag acggcgtgcc gctgttcctc gaggagttga cgaaaaccgt
     2521 tgtcgagagc ggcctgttgc gggatgccga cgatcgctac gagatcgcgg ggcagctttc
     2581 gccgacggcg atccccgcga cgctccacga ttcattgatg gccaggctcg atcacgtgac
     2641 ggcaagggct gtcgcccaga tggcggcgtg catcgggcgc gatttcgatc gctcgctgct
     2701 gacggcggtg tccggattga cggaggagcg cctccgcgag gcattggacg aactgcgccg
     2761 gaccgggctg atcgttgcaa ccggtatcgg cgccaacgag cgccacagtt ttcgtcatgc
     2821 tctcatccgc gatgccgcct acgaaagcct gctgaaaggc cgacgggccc agctgcatgc
     2881 cgcgatcgcc ggcgctctcg agcgatcctt tgccgacgtc gtcgctgcgc agcccgagat
     2941 cgtcgcgcat catttctccg aggctggcca acccgacaag gccatcacct attgggtgca
     3001 agccgggcaa cgcgcgactt tgcgctcggc caacgcagag gcattttcgc atctgcagaa
     3061 tggcctggag ctgctggatc agatcactga tgcgcgcgag cgcgatcgct gcgaattatt
     3121 gctccgcacg atattgggcg cggtctacgt cgccacaaaa ggatactcgg cggccgaaac
     3181 cgtggcgact ttcaagcggg cggcagacct gctgcaggcc acaggagatc tgcggcttcg
     3241 gctcgccgtc cataacgggc ttctggtcgg atactacaat ctcgcgcgct tcgacggcgc
     3301 tctcgacctg gcccaggaga cgttgagaca aggtgagaaa gacggcgacg atgccgttat
     3361 ctgcgtggga caccggatgg tggccgcggt ctgcaattcg gtcggcgagt tcgagcgagc
     3421 tgcccatcac gcgcgcagag gctgggagct gtaccggccg gaacgtcacg ggcttgcggc
     3481 gctcggtctc gtccacgaca cgggtctcgg ggccaaattg caccttgcgc ttgcactatg
     3541 tcacctcggg ttccagaacc agtcaaagca agccgccgcc gaggcgttgt cgctggcggc
     3601 agatctgcat cacgtgaaca gccaggccta tgcctggttt tttgccgcgg tattggtcaa
     3661 ctttgtcggc cgcaatcaca gcgcccttat cgaatacgcg cctcgactgc gcacatacgc
     3721 ccaacagcac gaaatgccgc aatgggctgc ttacggacgg gcgttcggcg ccgtgccgct
     3781 tgtaagctct ggccgcgccg gtgtcgcact ggacgagata accggtgcaa taggcgattg
     3841 cgagcgcatc cagaattttg tttttcgccc ggctcaactc actattcttg cttcggccga
     3901 gcttgccgtc gggcgcgccg atcgagccct cgccgccaca gccagcgcac tcgaaattgc
     3961 cgagcatacg cgcgagcatt ggttgaccgc cgagacgtgt cgcatccgcg gtcatgccct
     4021 cctgtcgatg gaagatccgg
//
LOCUS       DASSRL010000301         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1740463, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000301 DASSRL010000000
VERSION     DASSRL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740463"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFL49_06250"
     CDS             complement(<1..223)
                     /locus_tag="VFL49_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911611.1"
                     /translation="MARLTAATSAGEPQDGVPEKAARLLYALLWGFARIFSVLALAVT
                     WEILARSGTFTPFQLPALSAVLERIWSDAV"
     gene            complement(211..954)
                     /locus_tag="VFL49_06255"
     CDS             complement(211..954)
                     /locus_tag="VFL49_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7911612.1"
                     /translation="MKQAMIRYLPLLILAIAWEIVARLELVSTSALPPLSDVISAGIA
                     MVKDGELISNGASSLYRAAAGLALAVLIGSTVGIAMAWWKPVNVLVAPIVEIFYPLPK
                     SALIPVTVIWLGFGDGSKILLIFLGCMLPVTIGAFNGARSSEQVLVWSARSMGASRLR
                     MLWDVVVPSAMPEMLNGVRTALALSFILLVSSELIVAQRGFGYLIGFLGANGNYDGMY
                     AVVLTVAFLGFAADRLYLQITKRTLAWRD"
     gene            complement(951..1742)
                     /locus_tag="VFL49_06260"
     CDS             complement(951..1742)
                     /locus_tag="VFL49_06260"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911613.1"
                     /translation="MATAAQIVVDRVNHLYRPPRGREVLALDQVSLEVGNREFVALLG
                     PSGCGKSTLLYLIGGFLPVETGAIRIDGQNVGGPGPDRGIVFQHFALFPWKTVRGNIL
                     YGLERQGMPKEEREKRAMDFIELVGLAGFEDSYPSQLSGGMKQRTAIARTLAFDPKIL
                     LMDEPFGALDAQTRGLMQSELLRIWQRTPKTVIFVTHDVQEAVYLADRVAVMSARPGR
                     IKTIVDTKFDKNDPDIFKNKFFVEKVDEIWNLVKEEAIKAQGKRS"
     gene            1862..2683
                     /locus_tag="VFL49_06265"
     CDS             1862..2683
                     /locus_tag="VFL49_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7911614.1"
                     /translation="MANTLPEACRELALANHIVANEGVIDAFGHVSMRHPDNPKRYLL
                     SRSRAPELVTPDDFIEYDLDSQPGRDPGVGQYSERVIHGEIYKARPEVNSVCHHHCPA
                     FMPLLATGTDYMPIFHLGSVGGIRPPFWDQRDEFGDTNMLVIKPEEGASLARALGKHW
                     MVLMMRHGVTVAGTSVRDCVFRSVFSARNAAYQVMAMTVGSEIATLSPGETVLAGQIS
                     SKTTGLTRSWEYWSMRVANKAGGSAASSQPKSSQKRRAAPAKKSRSKQSRKKRRR"
BASE COUNT          609 a          893 c          907 g          536 t
ORIGIN      
        1 agacggcgtc gctccagatg cgctcgagca cggcgctcag tgccggtaat tggaacggcg
       61 tgaaagtacc gctgcgggcg aggatttccc aggtgaccgc aagcgcgagg acggaaaaga
      121 tgcgcgcaaa tccccaaagc agcgcataga gaagccgcgc cgctttttcg ggaacgccgt
      181 cttgcggttc gccggccgag gtcgctgcgg tcagtcgcgc catgcgagtg tccgtttggt
      241 gatctggagg tagagtcgat cggcggcgaa accgagaaag gcaacggtca ggaccaccgc
      301 atacatcccg tcatagttgc cgtttgcgcc caggaagccg atcagatagc caaatcctct
      361 ttgcgcaacg atcagctccg acgagaccag caggataaac gagagggcga gcgcggtgcg
      421 cacgccattg agcatctccg gcatggcgct cggcaccacg acgtcccaca gcatgcgcag
      481 ccggctggcg cccatgctgc gtgccgacca gaccagcacc tgctcgctcg accgcgcgcc
      541 gttgaacgcg ccgatcgtca ccggcagcat gcaacccaga aatatcagca ggattttcga
      601 cccgtcgccg aagccgagcc agatgacggt cacgggaatc agcgccgact ttggcagcgg
      661 atagaaaatt tcgacgatgg gcgcgaccag cacgttcacc ggcttccacc aggccatggc
      721 gatcccgacc gtgctgccga tcaggaccgc caaagcgagc ccggcggcgg cccgatacag
      781 cgacgatgcg ccgttgctga taagttcgcc gtccttgacc atggcgatgc cggccgagat
      841 gacatctgac aagggtggca aagcggacgt cgatacgagc tcgaggcgcg ccacgatctc
      901 ccaggcaatt gcgagaataa gaagcggaag atagcggatc atggcctgct tcatgaccgc
      961 ttgccctgcg ccttgatcgc ctcttccttc accagattcc agatctcgtc gaccttctcg
     1021 acgaagaact tgttcttgaa gatatcggga tcgttcttgt cgaacttggt gtcgacgatt
     1081 gtcttgatgc ggccgggtcg tgccgacatc accgcgaccc ggtcggcaag gtagacagcc
     1141 tcctgcacgt cgtgagtcac gaagatgacg gtcttcggcg tgcgttgcca gatgcgcagc
     1201 agctcactct gcatcaggcc gcgtgtctgc gcatcgagcg cgccgaatgg ttcatccatc
     1261 agcaggatct tcgggtcgaa ggcgagcgtg cgcgcgatcg cggtgcgttg tttcatgccg
     1321 ccggacagtt gggaaggata gctgtcctcg aaaccagcga gtccgaccag ctcgatgaaa
     1381 tccattgcgc gtttctcgcg ctcttccttg ggcatgccct gccgttcgag cccgtagaga
     1441 atattgccgc gcacggtctt ccacgggaaa agggcgaagt gctgaaaaac gatgccgcgg
     1501 tcaggccccg ggccgccgac attctggccg tcgatccgga tcgcgccagt ctcgaccggc
     1561 aaaaatccgc cgatgaggta gagcaaggtg gatttgccgc agccggacgg cccgagcagc
     1621 gcaacgaatt cgcgattgcc gacctcaagt gaaacctggt cgagcgccag cacctcgcgg
     1681 ccgcgcggcg ggcgatacag atgattgacc cggtcgacga cgatttgggc tgcagttgcc
     1741 atgcggaaag aagcgtcccc aaccggcaca atctgtcaag cgccgtccgt gctggttcga
     1801 cgccgatgat gtcctataat gcgggacctt aaaccttccg tactttcaag aggaaatacc
     1861 agtggccaac acgcttcctg aagcctgccg cgaactcgcg cttgcaaacc atattgtcgc
     1921 caatgaaggc gtgatcgatg ccttcggcca tgtcagcatg cggcatccgg ataatccaaa
     1981 acgctatctg ctgtcgcgct cgcgtgcgcc cgagctggtg acgccggacg acttcattga
     2041 atatgacctt gactcgcaac cggggcgcga tccgggcgtc gggcaatatt ccgagcgggt
     2101 catccacggc gaaatctata aggcgcggcc ggaagtgaat tccgtgtgtc accaccactg
     2161 tccggccttc atgcccctgc ttgccaccgg tacggactac atgccgattt ttcacctggg
     2221 ctctgtcggc ggcatcagac cgccgttctg ggatcagcgc gatgagttcg gcgacaccaa
     2281 tatgctggtg ataaagccgg aggagggcgc ttcgctggcg cgtgcgctcg gcaagcattg
     2341 gatggtgctg atgatgcgcc acggggttac ggtggccggc accagtgtgc gtgattgcgt
     2401 cttccgttcg gtgttctcgg cgcgcaatgc cgcgtatcag gtcatggcga tgacagtcgg
     2461 ctccgagatc gccacgcttt cacccggcga gaccgtgttg gcgggccaga tcagctccaa
     2521 gaccacgggt ctcacacgct cgtgggaata ttggtcgatg cgcgtcgcca ataaggctgg
     2581 gggatctgcg gcttcttctc agcccaaatc gagccaaaaa cggcgcgccg cgcctgccaa
     2641 aaagtcgcga tcaaagcaaa gccgtaaaaa gcggcggcgc tgatattgca ctttcctgcc
     2701 ggcagatgcc ggtgttgaat gcggctcata agggggcaca cgtcgacaag caaaagcacc
     2761 atgggaggaa cgcatgaaag ccttcacgac aataggaggc ctgctcggga ttgcggttgc
     2821 gctgtccgta gcccccgctg cggctgagga tcacctgaag gtggctatcg gccagatcaa
     2881 caactgggaa aatcaggctc cgaccctcgg ccaggacgcc ggcattttca agaagcacgg
     2941 gctcg
//
LOCUS       DASSRL010000302         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1705409, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000302 DASSRL010000000
VERSION     DASSRL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705409"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFL49_06270"
     CDS             <1..748
                     /locus_tag="VFL49_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7911615.1"
                     /translation="PSDAPKELLFKLHERLGIQHCVIVQPACHGEDNSVTADAIAATG
                     GSYKGIALVPLSVSDAELKRLDAAGLCGARFHYMTHLAQGPAINDVIAFARRLAAVGW
                     HLQIHMEADLIAELAPALRQSPVPVVIDHMGRINASLGLEQPAFKALLALLNDRNVWV
                     KVSGADRVTRQGPPYADAVPFARRLVAEFGDRCVWGSDWPHPNHRGPIPDDGVLVDIL
                     GEIAPGEALKAVLVDNPQRFYRFAPARQLT"
     gene            761..1561
                     /locus_tag="VFL49_06275"
     CDS             761..1561
                     /locus_tag="VFL49_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7911616.1"
                     /translation="MGGRLQDRIAIVTGAGCVGPGWGNGRAIAVRFAEEGARIFAVDR
                     DLDSVAETVERVKAAGGEIVTHPCDVTGAASVEAMVKTCVERFGRIDVLVNNVGGSAA
                     GGPAEMSEDVWDAQVDFNLKSVFLTLKHVLPVMERQKSGAVVNIASTSGLRWTGSAQV
                     GYAATKAGVIQLSRVVAVQYADKGIRVNTVVPGQLHTPMVEARLAKQRTGGDVEALLK
                     SRLARIPIGFMGDGRDTANAALFLASDEARFITGTEIVVDGGMTVRCD"
     gene            1884..2351
                     /locus_tag="VFL49_06280"
     CDS             1884..2351
                     /locus_tag="VFL49_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7911617.1"
                     /translation="MTQKFTLNVNGNVHTVDADPDMPLLYALRDDIGLNNPHFGCGLA
                     QCGACTVHLDGQPTRSCITAMSLVGDAKITTLAGLGTPEKPHPLQTAYIEEQVPQCGY
                     CINGWIMTAAALLRDKKKPTEAEIKDALSSLKCRCGTHISILKAVKRAAEMMG"
     gene            2358..4643
                     /locus_tag="VFL49_06285"
     CDS             2358..4643
                     /locus_tag="VFL49_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7911618.1"
                     /translation="MTKYEKMSPFTRRSVLLGGGALVVSVGAAISLETVLSIGKAHAQ
                     GVKPPLTPEQLSSYIAVNADGTVSAFFGKMDMGHGLHVAIGQMVAEELDVPFKNVKVI
                     MADTAVTVNQGGASGSTGIQNGGKQMRFAAAEARRVLVEMAASRLSVPAERLVVTDGV
                     VSASDDKTKRISYAELIGGKYFNVQLDWNKQYGNALYAPGKAQPKKPSEHKIVGQPIK
                     REDVAPKVFAQEDFVTDVKVPGMVHGRMIRPAVAGSVPVKVDESSVKDIAGVKVVWDK
                     GFLGVVADKEWDAIQAAQKLKVEWSDTPPPFPDQSALYDHIRKASVRKRQIEKENGNV
                     DEAFARAAKVIEAEYEWPFQSHASMGPACALVEIKDGQVTCWSGTQKSHFQQQGLAAT
                     LGVPLESVRVIWKPGPGSYGRNDADDAAMDAAVMAKAVGKPVRVQYMRDQGTGWDPKG
                     PASVHKVRAAIDASGKVTAYEFMSKAFSRIDVNTNGSKPIDTLAGQARGADLKSGDGF
                     GIPAESYAFENKRAGWETVPPLLDRASPLRTSHLRDPVGPQIHFASESFIDEVAAALN
                     VDPIEFRLRHVKDPRDIALLKAVTEKSGWQSRPSPRHDQSGNKVSGRGVAYSQRNGTR
                     VAVVAEVDIDRSTGKIWARKFTVAHDCGQIINPDGLVKCIEGNIVQGVSRTLWEEVTF
                     DRKAVTSVDWLTYPILDITETPGEVTVVLINHPEIAPTGAGEPSIRPVAAAIANAIFD
                     ATGVRIRRVPFSPDRVKAALS"
     gene            4704..4874
                     /locus_tag="VFL49_06290"
                     /pseudo
     CDS             4704..4874
                     /locus_tag="VFL49_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         1005 a         1496 c         1568 g          871 t
ORIGIN      
        1 gccgtccgat gcgccgaaag agctgctgtt caagttgcat gagcgcctcg gcatccagca
       61 ttgcgtgatc gtgcagccgg cctgtcacgg cgaagacaat tcggtgacgg ccgacgccat
      121 tgcggcgacg ggcggctcgt ataaaggcat cgcactcgtg ccgctgtcgg tttcggatgc
      181 cgagctcaag cggctcgacg ccgcgggcct ttgcggcgcc cgtttccact acatgacgca
      241 tctcgcccag ggtcccgcga tcaacgacgt cattgccttc gccaggcggc tcgccgccgt
      301 cggttggcat ctgcagattc acatggaagc ggacctgatc gccgagctgg cgccggcgct
      361 caggcagtcg ccggtgccgg tcgtgatcga tcacatgggg cgcatcaacg cgtcgctcgg
      421 cctcgaacag ccggccttca aggcgctgct cgcactcctc aacgacagga atgtgtgggt
      481 gaaagtgagc ggtgccgacc gcgtgacccg gcagggacca ccctatgccg atgcggtgcc
      541 gtttgcgcgc aggctggtgg ccgaattcgg cgaccgttgc gtctggggca gcgactggcc
      601 gcatcccaat catcgcgggc cgatcccgga tgacggggtg ctggtcgata ttctgggcga
      661 gatcgcaccg ggtgaagcgc tcaaagcggt gctggtcgac aatccgcaac gcttctatcg
      721 tttcgcaccg gcgcgtcaat tgacctgagg aacaaagagc atgggcgggc gcctgcagga
      781 ccgcatcgcg atcgtcaccg gcgccggctg cgtcggcccc ggctggggca atggccgcgc
      841 cattgccgtg cgcttcgccg aggaaggcgc caggattttc gccgtcgacc gcgatctcga
      901 cagcgtcgcg gaaaccgtcg agcgcgtgaa agcggccggc ggcgagatcg tcacccatcc
      961 atgcgacgtc accggcgccg cctcggtcga ggccatggtg aagacctgcg tcgagcgttt
     1021 cggccgcatc gatgtgctgg tgaacaatgt cggcggctcg gccgccggcg ggccggccga
     1081 aatgagcgag gacgtctggg atgcgcaggt cgatttcaac ctgaaaagcg tgtttttgac
     1141 gctcaaacac gtgctgccgg tcatggagcg gcagaagagc ggcgcggtcg tcaatatcgc
     1201 atcgacgtcg ggcctgcgct ggaccggctc ggcgcaggtc ggttacgccg cgaccaaggc
     1261 gggcgtgatc cagctttcgc gcgtcgtggc cgtgcaatat gccgacaagg gcatccgcgt
     1321 gaacaccgtt gtgccggggc aactgcacac gccgatggtg gaagcccgcc ttgcgaagca
     1381 gcgcaccggc ggcgacgtcg aggctttact gaaatcgcgc cttgcgcgca tccccatcgg
     1441 ctttatgggc gatggccgcg acacagcgaa tgcggcactg tttcttgcat cggacgaggc
     1501 acgtttcatc accggcaccg agatcgtcgt ggatggcgga atgaccgtgc ggtgcgacta
     1561 gaagccaaat tggtcccgcg tccccgctga tggcagtccg gatgcaaaaa atttccctgc
     1621 gcgtggtcca gagtaatcgc acgtattcag gcaccgcttt cgggattgaa gcggtctagg
     1681 gcgcacagcg cctgcgagat gttcgcttgg acccaaaatg gacgggcacc ggaacctcac
     1741 gccgatgata catcaatttt acgcttgagc cgttcccaag ctcggttttg ttgcctaggc
     1801 tttcttttca aaccgttggt ccacgaccgc ccgcgagctt cgcgacaaag cggcaaatcc
     1861 gctcaagcca gaaagcgacc cgcatgaccc aaaagtttac cctcaatgtc aacggtaacg
     1921 tccatacggt cgatgccgat cctgatatgc cgctgctcta tgcattgcgc gatgatatcg
     1981 ggctcaacaa tccacatttc ggctgcggac tggcgcaatg cggcgcctgc accgtgcatc
     2041 tcgatggcca gccgacacgc tcgtgcatca ccgccatgtc tttggtcggc gacgccaaga
     2101 tcacgaccct cgcaggcctt gggactccgg aaaagccaca tccgttacag accgcctata
     2161 tcgaggagca ggtgccgcaa tgcggctact gtattaatgg ctggatcatg acggccgctg
     2221 ctttgcttcg cgacaagaag aagccgacgg aagccgaaat caaggatgcg ctttccagcc
     2281 tcaagtgccg ctgcggcaca cacatcagca ttttgaaagc ggtcaagcga gccgccgaga
     2341 tgatggggtg aggcatcatg acaaaatatg aaaaaatgtc gcctttcacc cgccggtcgg
     2401 tcctgctcgg tggtggcgcg ctagtggtct cggtcggcgc agccatatcg ttggagaccg
     2461 ttttgtcgat cggcaaggcg cacgcgcaag gcgtcaagcc accgctgacg cccgaacaac
     2521 tatcatcgta tatcgctgtc aatgccgacg gcaccgtatc cgctttcttc ggcaagatgg
     2581 acatgggcca cgggctgcat gttgccatcg gtcagatggt ggccgaggaa ctcgatgttc
     2641 ctttcaagaa tgtcaaggtc atcatggccg acaccgccgt caccgtgaac cagggcggcg
     2701 cttcaggctc gaccggtatt cagaacggcg gcaagcagat gcgcttcgcc gcggcggaag
     2761 cgcgccgcgt gctggtcgag atggcggcaa gccgactatc cgtaccggcc gaaaggctgg
     2821 tcgttactga cggcgtggta agcgccagcg acgacaagac caagcgcatt tcctacgccg
     2881 agctgatcgg cgggaaatat ttcaatgttc agctcgactg gaacaagcaa tacggcaacg
     2941 cactctatgc gcccggcaag gcgcagccga agaaaccgag cgagcacaaa atcgtcgggc
     3001 aaccgatcaa gcgcgaagac gtggcgccaa aggtctttgc ccaggaggac ttcgtcaccg
     3061 acgtgaaggt gcccggcatg gtgcatgggc ggatgattcg tcccgcagtg gccggcagcg
     3121 tgccggtgaa ggtggatgaa agctcggtca aggacatcgc tggcgtcaaa gtggtgtggg
     3181 acaagggctt tcttggcgtt gtcgccgaca aggaatggga tgccattcaa gcagcgcaaa
     3241 aacttaaggt cgaatggtcg gatacgccgc cgccattccc ggatcagagc gcgctctacg
     3301 accacatccg taaggcgagt gtacggaagc gccaaatcga aaaagaaaac ggcaacgtcg
     3361 acgaagcgtt cgcgcgcgca gcaaaggtca tcgaagcaga gtacgaatgg ccgttccaat
     3421 cacatgcgag catgggcccg gcctgcgcac tggtcgagat caaggatggg caggtgacgt
     3481 gctggtcggg cacgcaaaag tcgcacttcc agcaacaagg cctcgcggcg acgctcggcg
     3541 tgccgcttga aagcgtccgc gtaatctgga agcccgggcc cggatcttac ggtcgcaatg
     3601 acgccgatga cgcggccatg gatgcggccg tgatggcgaa agcggtcggc aaacctgtac
     3661 gcgtgcaata catgcgcgac cagggcaccg gctgggaccc aaaaggaccc gcgtccgtgc
     3721 acaaggtccg tgcggcaatc gatgcctccg gcaaagtcac cgcctacgaa ttcatgagca
     3781 aggcgttttc gcgcatcgac gtcaacacca atggcagcaa gcccattgac accctggccg
     3841 ggcaggctcg cggagccgac ctcaaatcgg gagacggctt tggcattccg gccgaatctt
     3901 atgccttcga aaacaagcgg gccggctggg aaacggtccc gccattgctg gatcgcgcat
     3961 cgccgttgcg cacatcgcat ctgcgcgacc cagttgggcc gcagatccat ttcgccagtg
     4021 agtcgttcat cgacgaggtg gccgccgccc tgaatgtcga tccgatcgaa ttcaggctgc
     4081 ggcatgtcaa ggatccacgc gacatcgcgc tgcttaaggc ggttacggaa aaatccgggt
     4141 ggcaatcgcg gccgtcaccg cgacacgatc agagcggaaa caaagtgtcc ggccgtggcg
     4201 tagcttactc gcaacgaaac ggcacgcgcg tcgcggtcgt cgccgaagtc gacatcgatc
     4261 gctcaaccgg aaaaatctgg gcacgcaaat tcacggtggc gcacgattgt gggcaaatca
     4321 tcaacccgga cggcctggtg aaatgcatcg agggcaacat tgtccagggt gtcagccgca
     4381 ccttgtggga agaagttacc ttcgaccgca aagccgtgac cagtgtggac tggctgacct
     4441 atccaatcct cgatatcacc gagacgccgg gcgaagtgac cgtggtgctg atcaaccatc
     4501 cggaaatcgc gccgaccggt gccggcgagc catcgatccg accggtggcg gcggcgatcg
     4561 ccaacgccat cttcgatgcc accggcgtgc ggatacgacg ggtgccgttc tcgcccgatc
     4621 gcgtgaaagc cgcgctatcg taagccggca accatggtgg cacgccaacg gtggaagccc
     4681 gccttgcgaa gcagcgcacc ggcggcgacg tcgaggcttt gctgaaatcg cgccttgcgc
     4741 gcatccccat cggctttatg ggcgatggcc gtgacacagc gaatgcggca ctgtttctcg
     4801 catcggacga ggcacgtttc atcaccggca ccgagatcgt cgtggatggc gggatgacca
     4861 tgcggtgcga ctagctcatg ccgcgtggga atgcggtgaa aggccgtcgt ggctatgcct
     4921 ttcgatgcga tggtggcgtt
//
LOCUS       DASSRL010000303         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_900882, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000303 DASSRL010000000
VERSION     DASSRL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900882"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFL49_06295"
     CDS             <1..189
                     /locus_tag="VFL49_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HET7911619.1"
                     /translation="DQAGASKSASAAMANLAEAIQGLVHHMRTEQQMIRDWVDSQADQ
                     QREIKKLLEVMVREHVNR"
     gene            200..1228
                     /locus_tag="VFL49_06300"
     CDS             200..1228
                     /locus_tag="VFL49_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HET7911620.1"
                     /translation="MALARVRRGESGMNYWPGFVDALSTLILGIIFLLTVFVVVQFYL
                     QQEVAGKDTALSRLNAQIAQLTELLSLEKSGKTDLEDQLAQLRATLATTESERDRLKG
                     AAESAGAGAAAAQGKVSDLTGQLEGEKRISARALAQVEVLNQQIAALRRQLAALEQTL
                     DAAEKKDKDAQSRITELGQRLNLALAERVNELKRYRSDFFGKLREILGNRADVRIVGD
                     RFVLQSEVFFDTGKADLKPEGRAELDKVADALLELEKQIPTDLAWVLRVDGHTDVRPI
                     SGGVFKSNWDLSAARAISVVQYLIGKGVSPQRLVAAGFGEFQPIDLGKTEDAYRRNRR
                     IEFKLTER"
     gene            1225..1680
                     /locus_tag="VFL49_06305"
     CDS             1225..1680
                     /locus_tag="VFL49_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7911621.1"
                     /translation="MSGPTFTLRSYRASDEDAAIELWRRTWSSHYPHLDFNARQPWWR
                     ERWRNELVPTAQIAVAERNGTIIGFVTVDPKTMYLDQIVVAPEHWGSDVALALLDEAK
                     RLSPQGIHLLVNKDNFRAIRFYEKHGFVYAGEDKNPVSGLPVNRMTWRG"
     gene            complement(1677..3491)
                     /locus_tag="VFL49_06310"
     CDS             complement(1677..3491)
                     /locus_tag="VFL49_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET7911622.1"
                     /translation="MSYSSDGRGLGVADGPAPDERRKLNLRPLARLVPYVVRYRWRAA
                     AALAALIVASLTTLIVPVAVRRMIDFGFSDKAAQLIDSYFAVMVLIVAALAISSALRF
                     YLVTTLGERIVADLRSDVFAHLTRLSPAYFDSVRAGEIVSRLTADTTQIKSAVGASVS
                     IALRNLVLFVGGAGMMVVTSPRLSLFVLGAIPVIVLPLVGFGRAVRRRGRAAQDTLAD
                     ASGYANELIGATRTLQAFTNEKLARSRFATAVEQAYVAARGATKARAFLTAVVIFLVF
                     ASVVIVLWVGAQDVLAGEMTPGRLGQFVLYAVFAAGGLGELSQVWGEISQASGAAERL
                     SEILAVQPEIRSPPKPIALPNPASGDVKFHHVTFSYPARPNVPVLENLSFHVVPGEKL
                     ALVGPSGAGKSTIFQLILRFYDPLSGNITFDGLRLPDVDPAALRSRIALVPQDPIVFA
                     TSVRDNIQFGRPEASKAEIENAAEAAHAAEFVRRLPLGFDTPIGERGVTLSGGQRQRI
                     AIARAILRRAPLLLLDEATSSLDAESETLVQQALKRLMLNRTSIVIAHRLATVQSCDR
                     ILVIDNGRIVEEGTHTSLASAGGLYARLAKLQFEMGAA"
     gene            3646..3882
                     /gene="rpmE"
                     /locus_tag="VFL49_06315"
     CDS             3646..3882
                     /gene="rpmE"
                     /locus_tag="VFL49_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET7911623.1"
                     /translation="MKQDIHPSYHTIKVVMTDGSEFTTRSTWGKEGDVLHLDIDPKSH
                     PAWTGGQQHLVDRGGRVSRFQKKFSGFLKADEKK"
     gene            complement(3949..>4272)
                     /locus_tag="VFL49_06320"
     CDS             complement(3949..>4272)
                     /locus_tag="VFL49_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HET7911624.1"
                     /translation="LTTRLMQLASWLLLHRAVKEGEMSLAQAGKEKSKVKLAAADSHD
                     PNNIELLPPALSDLIARSQKLYAKVRRLDATIYNQGPAERPAAANPVERQLGLLKAAF
                     GTERV"
BASE COUNT          895 a         1323 c         1305 g          749 t
ORIGIN      
        1 gaccaggcgg gcgcgagcaa atcagcatcc gcggccatgg ccaatctggc cgaggcgatc
       61 caggggttgg tgcaccacat gcgcaccgag cagcagatga tcagggactg ggtcgactca
      121 caggccgacc agcagcgaga gatcaagaag ctgctcgaag tgatggtgcg cgagcacgta
      181 aaccgttgag tcaagactga tggcgcttgc aagagtccgc cgcggcgaga gcggcatgaa
      241 ctactggccg ggcttcgtcg atgctctgtc gacgctgatt cttggcatca tcttcctgct
      301 caccgtcttc gttgtggtgc agttctatct gcagcaggaa gttgccggaa aggatacagc
      361 gctcagccgc ctgaacgcgc agatcgcgca actcaccgag ctgctgtcgc tggaaaagtc
      421 gggaaaaacc gatctcgagg accaactggc gcagttgcgc gcgacactcg ccaccacgga
      481 aagcgagcgc gaccgcctca agggcgctgc cgaaagcgcc ggcgccggtg ctgcagctgc
      541 tcagggaaag gtgtccgacc tcaccggcca gctcgaaggc gaaaagagga tctcggcgcg
      601 cgcgttggcg caggtcgagg tgctcaatca gcagatcgcg gcgctgcgcc gtcagctggc
      661 ggcgctggag cagacgctcg acgctgcgga gaagaaggac aaggacgcgc aaagccgtat
      721 taccgagctt ggacagcggc tcaatctcgc cttggccgaa cgcgtgaatg aactcaagcg
      781 ctatcgttcg gatttcttcg gaaaactccg cgagattttg ggcaaccggg ccgacgttcg
      841 tatcgttggc gaccgttttg tcctgcagtc cgaggtattt tttgacaccg gcaaagccga
      901 cctcaagccg gaaggccgcg ccgaactcga taaggtcgcc gatgccctgc tcgagctgga
      961 aaagcagatc ccgaccgatc tcgcctgggt gctgcgcgtc gacggccaca ccgatgtccg
     1021 gcctatctcc ggcggtgtgt tcaagtcgaa ctgggacctt tcggcggccc gggcgatctc
     1081 ggtcgtgcaa tatctcattg gcaagggcgt ttccccgcag cggctggtgg ctgccggctt
     1141 cggcgagttc cagccgatcg atcttggcaa gaccgaggat gcctacaggc gcaatcgccg
     1201 catcgagttc aagctgaccg aacggtgagc gggcccacct tcaccctgcg ctcctaccgc
     1261 gcctccgacg aagacgctgc gatcgaattg tggcgccgca catggtccag ccactatccc
     1321 catctcgact tcaacgcccg tcagccgtgg tggcgcgagc gctggcgcaa tgagctcgtg
     1381 cctacggcgc aaatcgccgt ggcggaacgc aacggaacca tcatcggttt cgttaccgtc
     1441 gacccgaaga caatgtatct cgaccagatc gtcgtcgcgc ctgagcactg gggctcggat
     1501 gtcgcgctgg ctttgctcga tgaagccaag cgcctttcgc cgcagggaat ccatcttctt
     1561 gtcaacaagg acaattttcg cgccatccgc ttctacgaaa agcacggctt cgtgtatgca
     1621 ggcgaagaca agaatcccgt atcgggcttg cctgtgaacc gcatgacgtg gcgcggttag
     1681 gcggcaccca tctcgaattg caacttcgcc agacgtgcat aaaggccgcc cgccgaagca
     1741 agcgatgtat gcgtcccttc ctcgacgatg cgaccgttat cgatgaccag gatgcgatca
     1801 cacgactgca ccgtggcgag ccgatgcgcg atcacgatgc tggtgcggtt gagcatcagc
     1861 cgcttgagcg cctgctgtac cagggtttca ctctcggcat cgagtgatga tgtcgcttcg
     1921 tcaagcaaca atagcggcgc tctgcgcagg atagcgcggg cgatggcgat ccgctgccgc
     1981 tgtccgcctg acagagtaac gccgcgttca ccgatcgggg tgtcaaagcc gagcggcagg
     2041 cggcggacaa attccgcggc atgcgcggct tccgccgcgt tctcgatttc agccttgctc
     2101 gcttccggcc gcccgaactg aatgttgtcg cggacgctcg tcgcgaacac gatcgggtct
     2161 tgcggcacga gcgcgatgcg cgagcgcagc gccgcgggat cgacatcggg cagacgcaag
     2221 ccgtcaaaag ttatgtttcc ggataagggg tcatagaatc gcaggatcag ctgaaaaatg
     2281 gtgctcttgc cggccccgga cggcccgaca agcgcgagtt tctcgcccgg cacaacgtga
     2341 aacgaaaggt tttcgagaac cggaacgttc gggcgcgcgg gataagaaaa agtgacgtga
     2401 tgaaacttga cgtcgccgct ggccggattc ggcaaagcga ttggctttgg tggcgatctg
     2461 atctccggct gcaccgccag aatttcagac agtcgctcgg cggcacccga ggcctgcgag
     2521 atttcacccc agacctggct taactcaccg agaccgcccg ccgcaaacac cgcgtagagc
     2581 acaaactggc cgagccgacc gggcgtcatt tcgcctgcga gcacatcctg tgctccaacc
     2641 cacaggacga tcacgacgct ggcaaagacg agaaatatga cgaccgctgt gagaaacgca
     2701 cgtgccttgg tcgcgccgcg ggcggcgacg taagcttgct cgaccgccgt agcgaaacgc
     2761 gacctcgcca gcttctcgtt ggtgaaagcc tgcaaggttc gcgtcgcgcc gataagctcg
     2821 ttggcatagc cggacgcgtc ggccaatgta tcctgcgccg cgcgtccgcg ccggcgcacc
     2881 gcgcgcccga agcctacaag cggcagcacg atcaccggaa ttgcccccaa tacgaacaaa
     2941 gacagccgcg ggctggtcac gaccatcatg ccggcaccgc caacaaacag gaccagattg
     3001 cgcaacgcga tcgacaccga agcgcctacg gccgatttga tctgagtcgt atcagcagtg
     3061 agccgcgata cgatttcacc tgcgcgcacg ctatcgaaat aggccggcga cagccgcgta
     3121 agatgcgcga atacgtccga gcgcaaatcg gcgacgatac gttcgcctaa tgtcgtcacc
     3181 agataaaatc gcagcgcgct cgaaattgcg agcgcggcga cgatgagaac catcaccgca
     3241 aaatagctgt cgataagctg cgcggccttg tcggaaaacc cgaagtcgat catgcgacgg
     3301 acggccacgg gtacaatcaa ggtcgtcaac gaagcaacga tcagtgcggc aagcgccgcg
     3361 gcggcacgcc agcgatagcg cacgacatag ggaaccaggc gggcgagcgg cctcagattc
     3421 agcttgcggc gctcgtccgg agctggtccg tctgcgacgc cgaggccccg tccatcgctc
     3481 gaataactca tgcgacgcct ctcaccgccc catttgccgc ttccttaatg ccttgcgggc
     3541 ttggcggcgg tcaatcacag ccgcttgccg cctttggccg cctcctgtat agggagcgca
     3601 aaatcaggca tatcggcgca ttctgaagcg cccggaattg aagagatgaa acaagatatc
     3661 catcccagct atcacaccat caaggtggtg atgacggacg gcagcgagtt caccacgcgc
     3721 tccacgtggg gcaaggaagg tgacgtcctg cacctcgaca tcgaccccaa atcgcatcct
     3781 gcgtggaccg ggggccagca gcatctcgtt gatcgcggcg gccgcgtgtc gcgcttccag
     3841 aagaaattct ccggcttcct gaaggccgac gagaaaaagt aaagactgta ccggaagcca
     3901 aaaacccccg cggatgcggg ggtttttgtt ttgccgaaat ccaggcggtc agacgcgttc
     3961 agtgccgaag gccgccttga ggagaccaag ctgccgttcc accggattcg ccgcggcagg
     4021 acgttcggcc ggaccttgat tgtagatcgt tgcgtcgagc cggcgcactt ttgcgtaaag
     4081 cttctgcgag cgcgcgatca gatcgctaag cgccggcggc aacaactcga tattgttcgg
     4141 atcgtgactg tcggcagccg ccagcttgac cttgctcttt tccttgccgg cctgggcaag
     4201 cgacatttcg ccttccttta ccgcgcgatg gagcaacagc caggaggcaa gttgcattag
     4261 ccgagtggtg ag
//
LOCUS       DASSRL010000304         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_918248, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000304 DASSRL010000000
VERSION     DASSRL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918248"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1995
                     /locus_tag="VFL49_06325"
     CDS             232..1995
                     /locus_tag="VFL49_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7911625.1"
                     /translation="MTTQAPTLGDVQTTRVDAPAWLRGIGVRQVRLACGLIMFAYIFS
                     HFFNHALGNISYTLMQEWLWYHIWWWRIPLVNATLYSAAVIHFSLGLWALYQRRHFRY
                     TAAEITQLVLGLSIPLWLASHFGVTRVAGWLYDWPPLPYANPLFGYWVTRQHMIAVQF
                     ILMTVAWIHACIGLYFWLRMKPFFRWAAPILLSIAVLLPPLAMLGAHQGAQEVIRRAK
                     DPAWRAQNIKPIPRDKRQVIDTITLFYFPIGYAAAILLVFAARGVRTLNERRRGIVTV
                     SYPGRRVRIPKGLSVLEASLRNKVPHASVCGGRARCSTCRVKVVSDRSALPKPSGRES
                     FVLARVGAGKDPSIRLACQLRPQTDVAVIPVMPPNIGADFVRNRQRIHVGEEHFVVSM
                     FVDMRGSTSLSEARLPFDVVFLINRFVETVSRAVTDAGGEPNQFVGDGVLALFGLNSD
                     RATACRQALRAASLVESNVAHLNHQFATEVSEPVQYGIGIHAGEVIIGDIGFRGRTVF
                     TALGDSVNVAARLQELTKRLDCKAVISEEVCKLSGIRPDALTKTDVEVRGHEKPMSVR
                     TTDDPTVLAGLIDPQQAVAAE"
BASE COUNT          337 a          673 c          646 g          399 t
ORIGIN      
        1 agggtaaggg cccgtttcgg cgccttcata accgccgtca tgcccgcgca tagccgttcg
       61 aagaacggcg tgctttcgca cgcctatgtc gcgggcatcc acgtcttcgg ccgtgcaaaa
      121 aagtaaggcg tggatggccg ggacaagccc ggccatgaca ggggcctacc ttgtattcgt
      181 ccggcaattc ccggacgatg gctcggggac aggcgggcga gctatcggtt catgacaact
      241 caggcaccca ctttgggtga cgtgcagacg acacgcgttg atgcgccggc gtggctgcgc
      301 ggcatcggcg tgcggcaggt tcgccttgcc tgcggactga tcatgttcgc atacatcttc
      361 tcgcacttct tcaatcacgc actcggcaat atctcctaca cgctgatgca ggagtggctc
      421 tggtatcaca tctggtggtg gcggattccc ctcgtcaacg cgacgcttta ttccgccgcc
      481 gtgattcatt tctcgctcgg gctctgggcg ctctatcaac gccggcattt ccgctacacc
      541 gcggccgaga tcacgcaatt ggtgctcggg ctgagcattc cgctctggct cgccagccat
      601 tttggcgtga cgcgcgtcgc cggctggctc tacgactggc ctcccttgcc ctacgccaac
      661 ccactgttcg gttattgggt gacgcggcag cacatgatcg ccgtgcagtt catcctgatg
      721 accgtcgcct ggatacatgc ctgcatcggc ctctacttct ggctgcgtat gaagccgttc
      781 ttccgctggg ccgcccccat tctcctgtcg atcgccgtgc tgttgccccc gctggcgatg
      841 ctcggcgcgc accagggcgc gcaggaagtg atccggcgcg ccaaggatcc ggcctggcgg
      901 gcgcagaaca tcaagccgat cccgcgcgac aagcggcagg tcatcgacac catcacgttg
      961 ttctatttcc cgatcggcta cgcggcggcg atcctgctgg tattcgccgc gcgcggggtg
     1021 cgcacgctca acgagcgccg gcgcggaata gtcaccgtgt cctatccggg ccgccgggtg
     1081 cgcattccga aaggcttgag cgtgctcgag gcaagcctgc gcaacaaagt tccgcacgcg
     1141 agcgtctgcg gcggtcgcgc ccgctgctcg acctgccgcg tcaaggttgt cagcgaccgc
     1201 agcgctttac caaaaccatc cgggagggaa tccttcgtgc tggcacgcgt cggcgccgga
     1261 aaggatccct cgatccgcct ggcctgccag ctgcggccgc agaccgacgt cgcggtcatt
     1321 ccggtcatgc cgccgaatat cggcgccgat ttcgtgcgca accgccagcg catccacgtc
     1381 ggcgaggagc actttgtcgt cagcatgttc gtggacatgc gcggctcgac cagcctttcg
     1441 gaagcgcgat tgccgtttga cgtggtattt ctgatcaacc gctttgtcga gacggtgtca
     1501 cgcgcggtga ccgacgccgg cggcgagccg aaccagttcg tcggcgatgg cgtgctcgcg
     1561 ctgttcggcc tcaactccga tcgggcaacg gcctgccggc aggcactgcg ggccgcatcg
     1621 ctcgttgagt cgaacgtcgc ccatctcaac caccagttcg cgaccgaagt gagcgagcct
     1681 gttcagtacg ggatcggcat tcacgccggc gaggtgatca tcggcgacat cggcttccgt
     1741 ggccgcacgg tgttcacggc gctgggcgat tccgtcaacg tcgccgcgcg gctgcaggag
     1801 ctaacgaagc ggctcgactg caaggcggtc atttcggaag aggtttgcaa gctgtccggg
     1861 atccggcccg acgcactgac aaaaacggat gtcgaagtcc gcgggcatga aaagccgatg
     1921 agcgtgcgca cgacagacga cccgaccgtg ctcgcgggcc tgatcgatcc gcagcaggcg
     1981 gtggctgcgg agtgattaat gcagccatgt ccggatttgt ccccatgcat cgtcatgccc
     2041 ggcactcggg tccgg
//
LOCUS       DASSRL010000305         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_445887, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000305 DASSRL010000000
VERSION     DASSRL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445887"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2395)
                     /locus_tag="VFL49_06330"
     CDS             complement(116..2395)
                     /locus_tag="VFL49_06330"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET7911626.1"
                     /translation="MPAKPPAGRSRPTFTDQEALQFHSIGRPGKLEVIATKPMATQRD
                     LSLAYSPGVAVPVLAIAEDESRAYDYTTKGNFVAVITNGTAILGLGNRGALAAKPVME
                     GKAVLFKRFADIDSIDLEVSSEDPDEIVNCVKLLGKGWGGINLEDIKAPECFVIEQRL
                     RELMDIPVFHDDQHGTAIIAAAGLLNALDLTGREIATTKLVCNGAGAAGIACLELLKA
                     IGFKPDNLILCDTKGVIYQGRKDGMNQWKSGYAVKTSARTLAQALDGADVFFGLSVKG
                     AVTQDMVKKMAPKPIIFAMANPDPEITVEEVAAVRDDAIMATGRSDYPNQINNVLGFP
                     YIFRGALDVRASAINMEMKIAAARALAELAREDVPDEVASAYGARPKFGADYIIPVPF
                     DPRLISYVPPAVAKAAMDTGVARKPIVDMDAYRHQLSARRDPIAGTLVQVFNRLRRQP
                     RRVVFAEGEEEQVIRAAASFVHQGLGTALLVGREDRVRQSARETGIEIGEGIEIVNAR
                     MSTRNTVYMNFLYERLQRHGYLMRDCQRLVNQDRNHFAACMVALGDADAMVTGVTRNF
                     SVALEDIRHVIDPKPGHRVIGVSVVVARGRTVLVADTAVTEMPEAAEIADIAVEAAGV
                     ARRLGYEPRLALLAFSTFGHPPGERSARVIEAVKILDSRRVDFEYDGEMAADVALNPE
                     LVAAYPFCRLSGPANVLVMPAFHSASISTKMLQELGGATVIGPLLVGLDRPVQIVQLG
                     AKDNQLVNMAALAAYNVSG"
     gene            2558..2878
                     /locus_tag="VFL49_06335"
     CDS             2558..2878
                     /locus_tag="VFL49_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911627.1"
                     /translation="MIRFLLRFIGLICLAGGFILLIYDGTKSIAGNMIFLTDVRTLWE
                     LVNPASLARLKPIIEPYAGGFLWDPLMVTFLAAPSWSVFGVLGILFILLGRKKKPLIG
                     YVRP"
BASE COUNT          521 a          928 c          960 g          559 t
ORIGIN      
        1 ctcgaccgcg cgcttgaagt cgggcgcgcc gtcctcgtcg atcggggtcg cgatcgcggc
       61 gatcacgccg gaaaggctct tggtcaacgt cgtctcctgt ctgtcagtct tctagctagc
      121 cactcacgtt ataggcggca agcgccgcca tattgaccag ctggttgtcc ttggcgccga
      181 gctggacgat ctggaccggg cgatcgagcc cgaccagcaa cggcccgatc acggtggcgc
      241 cgccgagctc ctgcaacatc ttggtcgata tcgacgccga gtgaaatgcc ggcatgacca
      301 acacgttggc cgggccggac agccggcaaa acggataggc tgccaccagc tcaggattga
      361 gcgccacgtc ggcagccatt tcgccgtcgt actcgaaatc gacccgcctg ctgtcaagaa
      421 tcttcaccgc ctcgatcacc cgtgccgatc gctcgccggg cggatgcccg aaggtggaga
      481 aggcaagcag agcgagacgc ggctcatagc cgaggcggcg cgcgacgccg gcggcctcga
      541 cggcgatgtc ggcgatctcg gccgcttcgg gcatctcggt gaccgcggtg tcggcgacga
      601 gcacggtgcg gccgcgcgcc accaccaccg aaacgcctat gacccgatgt ccgggcttgg
      661 gatcgatgac gtgccggata tcttcgagcg ccaccgaaaa attgcgggta acgccggtca
      721 ccatcgcgtc ggcatcgccg agcgcgacca tgcaggccgc gaaatggttg cggtcctgat
      781 tcaccagccg ctggcaatct cgcatcaggt agccgtgccg ctgcaggcgc tcgtagagaa
      841 aattcatgta caccgtgttg cgggtcgaca tgcgcgcgtt gacgatctcg ataccctcgc
      901 caatctctat gccggtttcg cgcgcggact ggcgaacgcg atcctcgcgc ccgaccagga
      961 gtgcggtgcc caggccctgg tgcacgaagc tggccgccgc gcggataacc tgctcctcct
     1021 cgccttcggc aaagacgacg cgccgcggct gccggcgcag gcgattgaag acctggacca
     1081 gcgtgccggc gatcggatcg cgccgcgcgc tcaattggtg ccggtaagcg tccatgtcga
     1141 cgatcggctt gcgggcgaca ccggtgtcca tcgccgcctt ggcgaccgcc ggcggcacgt
     1201 aggaaatcaa ccgcggatcg aatggcaccg ggatgatgta gtccgctccg aatttcggac
     1261 gtgcgccgta ggcgctcgcg acctcatccg gaacgtcctc gcgcgcaagc tcggcgaggg
     1321 cgcgcgcagc ggcgatcttc atttccatgt tgattgcgga cgcccgcacg tcgagcgcgc
     1381 cacggaaaat ataagggaag ccgagaacgt tgttgatctg gttcgggtag tcggagcgcc
     1441 cggtcgccat gatggcgtcg tcgcggaccg cggcgacttc ctcaacggtg atttcggggt
     1501 ccggattggc catcgcgaag atgatcggct tcggcgccat tttcttcacc atgtcctgcg
     1561 tcacggcgcc tttaacggag agtccgaaga aaacatcggc gccgtccagc gcctgcgcca
     1621 gcgtgcgtgc cgaggtcttc acggcatagc ctgacttcca ctggttcatg ccgtccttgc
     1681 ggccctggta gatcacgcct ttggtgtcgc agaggatgag attgtcaggc ttgaagccga
     1741 tcgccttgag caattcgagg caggcgattc cggcggcgcc tgcgccgttg cagacgagtt
     1801 tcgtcgtggc gatctcgcgc ccggtcagat cgagtgcatt gagcaggcct gcggcagcga
     1861 taatcgcagt gccgtgctgg tcgtcatgaa ataccgggat gtccatcagc tcgcgcaggc
     1921 gctgctcgat cacgaagcac tccggcgcct tgatatcttc caggttgatg ccgccccaac
     1981 ccttgccgag aagcttcacg caattgacga tctcgtcggg gtcctcggat gagacctcca
     2041 gatcgatcga atcgatgtcg gcaaagcgtt tgaagaggac tgccttgcct tccatcaccg
     2101 gcttcgccgc aagcgctccg cggttgccga ggccgagaat ggcggtgcca ttggttatga
     2161 cggcgacgaa attgcccttg gtcgtgtagt cgtaagcgcg actttcatcc tcggcgatcg
     2221 cgagaacggg gaccgcgacg cccggcgagt aggcgagcga caggtcgcgc tgggtggcca
     2281 tcggcttggt agcgatgact tcgagcttgc ccggccggcc gatactgtgg aattgcaggg
     2341 cttcctggtc ggtaaaggtc ggccggctgc ggccggcagg gggtttggcg ggcattgata
     2401 ctccatcgac acaaccggta cgccggcgtg atcgacgtta gcggatgcaa ccgcgcggac
     2461 tcaagggaag gtgataccgt cctcatactg ttgaacgaag caaggggccg gggtaatcgg
     2521 tgccgggaag accgattcct ttcggctggc ggcggctatg atccgatttc tcctgcgctt
     2581 catcggcctg atctgcctcg caggcggatt catcctgctg atctacgacg ggaccaagtc
     2641 aattgccggc aacatgattt ttctcaccga cgtgcggacg ctgtgggagc tggtcaatcc
     2701 cgccagcctg gccaggctca agccgatcat cgagccttac gcgggtggtt tcctctggga
     2761 tcccttgatg gtgacattcc ttgcggcgcc gagctggagc gtgttcggcg tcctcggcat
     2821 tctgttcatt ctgctggggc ggaagaagaa gccgctcatc ggctatgtaa ggccgtaaac
     2881 accgccaccg tgccggagac gtgatccgcc cggccattcc ggcagcgccg gtcccgacat
     2941 atattgcggc caacgagtgc aggaggac
//
LOCUS       DASSRL010000306         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_358032, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000306 DASSRL010000000
VERSION     DASSRL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358032"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1347)
                     /locus_tag="VFL49_06340"
     CDS             complement(115..1347)
                     /locus_tag="VFL49_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7911628.1"
                     /translation="MHNSAKFAAVLATAALAYVPAPAAAQSCEDLILGAALSATGIYA
                     ANGNNTKNGYEFAVKKINDAGGIKIGGKCYHLKIKYYDDESTPARAAQLVERLIDQDK
                     VKFMLGPYGSPLTKAILPVIEKYKIPLVQGEAASRSLFTQGYKYQFGIVATSEKYLTP
                     VIDMAANLAKKAGKNPSAVKVAMIYQDDAFSLDVRQGVVEQIKKHNMHTVIDDRMPKD
                     LNDITTFFTKVKALQPDVLLISGHEKGAATAARQMGEFKTSVPLIGITHCESAKIVSD
                     FPKIAEGVVCPTQWDETMKASDPMFGTAPEYNKAMMAAHPEYKVVPYQTAQATAAVYV
                     WADAFKRAQSLDTEKVREALTKTDLQTFYGGVKFAEDGSNPGKEIVMRQIQHGKFIVV
                     SPTEVAAAPLVYPRDAHY"
     gene            complement(1491..1934)
                     /locus_tag="VFL49_06345"
     CDS             complement(1491..1934)
                     /locus_tag="VFL49_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911629.1"
                     /translation="MDTDALRRLHYAAAIACGVFAALVVHILLTVFGLGLDAVLRDAA
                     TGTRQQFVSAVAWWAIGGAGFAGGWAAGAYLIAAARERELIYRLAQRFLIGLVFVAAT
                     AGGIMSKTGNLGGTVDVIAGLAALGLGLICAFCGARLAYLNAEQV"
BASE COUNT          401 a          626 c          656 g          476 t
ORIGIN      
        1 ctgttccatc gcggacgtcc gtcgttgaga tggacggccc tatcgcattg cgatcgggcc
       61 gcgcacggaa gcggagagga agcgggcaga atgccgcccg cttccgtctt cggctcaata
      121 gtgcgcgtcg cgcggataga cgagcggtgc ggcggcaacc tccgtcggcg agacgacgat
      181 gaacttgccg tgctggatct gacgcatcac gatttctttg cccggattgc tgccatcctc
      241 ggcaaatttc acgccgccat agaaggtctg caggtctgtc ttcgtcagcg cctcgcggac
      301 cttttccgtg tcgagcgatt gtgctcgctt gaatgcgtcg gcccagacat aaaccgccgc
      361 ggtggcttgc gccgtctgat aaggcacgac cttgtactcg ggatgcgcgg ccatcatcgc
      421 cttgttgtac tcgggcgcgg ttccgaacat cggatcgctc gccttcattg tttcatccca
      481 ctgcgtggga cagacgactc cttccgcgat ctttgggaag tcgctgacaa tctttgccga
      541 ctcgcaatgt gtgatgccga tcaagggtac cgaggttttg aactcaccca tttgccgagc
      601 cgcagtcgcc gccccttttt cgtgacccga aatgagcagg acatccggct gcagcgcctt
      661 caccttcgtg aagaaggtgg tgatgtcatt gaggtctttc ggcatgcgat cgtcgatgac
      721 cgtatgcatg ttgtgtttct tgatctgctc gacgacgccc tgacggacgt ccagcgagaa
      781 tgcgtcgtcc tggtagatca tcgccacctt gacggctgac gggttttttc ccgctttctt
      841 cgcaagattc gccgccatgt cgatgaccgg cgtcagatat ttttccgacg ttgcgacgat
      901 tccgaactgg tatttatagc cctgcgtaaa gagcgacctc gaggcggcct cgccctgcac
      961 gagaggaatc ttgtattttt cgatgacggg caaaattgct ttggtcaacg gcgagccgta
     1021 tggacccagc atgaacttga ccttgtcctg gtcgatcagg cgctcaacca gctgtgcggc
     1081 gcgcgccggc gtcgattcgt cgtcgtagta tttgattttc aggtggtaac atttgccgcc
     1141 gattttgatg ccgccggcgt cgttgatttt tttgacggcg aattcgtaac cgttcttggt
     1201 gttgttgcca ttggcggcgt aaatgccggt ggccgaaagg gcggctccga ggatcaggtc
     1261 ttcacacgat tgcgccgcag cgggtgcggg tacataggcg agtgcggccg ttgccaacac
     1321 ggccgcaaat tttgcgctgt tgtgcattgg ttgttcctcc catgaaactg cgtgtctccc
     1381 ccggctggtt ggttccagtc cttgataatt gaaagtatag cgcgaatacg ggacgggaca
     1441 acgaatagtc gtgcgttcgg aggaccccgg ggacggagcc ggtccttcgg ctagacctgt
     1501 tcggcgttga gataagcgag ccttgcaccg caaaaggcgc agatcagccc caggccgagt
     1561 gccgcgagcc ctgcgatgac gtcgacggtg ccgcccagat tgccggtctt gctcatgatg
     1621 ccccccgcag tcgcggccac gaacacgagc ccgatcagaa accgctgggc cagccgatag
     1681 atcagctcgc gctcacgtgc ggctgcaatc aggtaggctc cggcggccca gccgcctgca
     1741 aatcctgcgc cgccgattgc ccaccaggcc acggcggata cgaactgctg cctggtgccg
     1801 gttgcggcat cgcgaagcac cgcgtcaaga ccaagaccaa acacggtcag gaggatgtgc
     1861 acgacaaggg cagcaaagac gccacaggca attgccgcgg cgtaatgcaa tcggcgcaga
     1921 gcgtcggtgt ccatcggctg ccgttgaaag gggacccggg aggtcgtctt gcgggcacaa
     1981 tagccgatca aagcggcgag cgtgaatacc ctttcgaaag ctcagctgca tcggcgtggt
     2041 ctggcgaaat accggtctca aggcttgcgt cgatttcctg gtcgacgatg tagcgcggcc
     2101 gcgactttgt ctcgaaatag attttgccga catattcgcc gatgactgca atggcgaag
//
LOCUS       DASSRL010000307         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1040863, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000307 DASSRL010000000
VERSION     DASSRL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040863"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFL49_06350"
     CDS             <1..331
                     /locus_tag="VFL49_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7911630.1"
                     /translation="GIHVAKVFADLGLDKALGPKIQLRDRGPDGLKEVAAGEIDIGLG
                     QISEILANKDVVLVGPLPPAIQGAVTFSGAIHSQAKDRAAAKALIDLLTSPEAKEKFK
                     AVGFTVS"
     gene            375..1364
                     /locus_tag="VFL49_06355"
     CDS             375..1364
                     /locus_tag="VFL49_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7911631.1"
                     /translation="MTKLTMPRRDFLTIAGGALAAAATGMRANAEGAQINFGYQNTSW
                     GTIGMIAGSEDLFKKAGANVAIHTFDSGKTTRDAMISGRIDIGVIGATPFVIGAAKGE
                     LEAIGLALYGAKTLAVVAGLKSGINSIKDLKGKKVGSQPGSATDHVFLTKILPKAGLT
                     KDDVQMVNVRFQNHISALASGSIDAFAGVEPFPSVAEVEKLGKVITDYSNYDLQPIIL
                     AANKSVIEKNRDGVVAFMRGWLAAVKIYQDKRDLATSIVLKHFKDQGFSVDDKVIKLM
                     LSKIDVNPEFGPQLKTYLTEEAQTLLEQKKISAIPDWNTRLNSSILSAARAKA"
     gene            1576..2346
                     /locus_tag="VFL49_06360"
     CDS             1576..2346
                     /locus_tag="VFL49_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7911632.1"
                     /translation="MKRALSIGLPLATFAVIAAAWQMLATYGGFPPKLVPGLGKIAEA
                     FVRLAGDGTLLAATIATLYRLGAGFLIAAVIGVTLGIAMGRRQWIEDTLLPMVTFLYP
                     IPGLAYAPLFVLWFGLGDVPTILLTGVASSFTVIINAWKGVKAVKPVWLRSAEVMGAR
                     GRDIFRRVVLPGALPYIMIGLRLGLAQAWRILIAVEMLMSVKRGLGWLIFGAQTFLNT
                     DVMLATIAVIGAIGILLEWQVFGRIERWTVVRWGMVAA"
     gene            2343..>2913
                     /locus_tag="VFL49_06365"
     CDS             2343..>2913
                     /locus_tag="VFL49_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7911633.1"
                     /translation="MRKGFSWIAFARGVAGIAVLLIIWEIFARSGMFSPAMTPPLESV
                     GRVIWTMLADGTFFRNALATLVRVFIGLGISFAVAVPLGVMMARYWVAERIFLPVLSV
                     LLPIPSLAWVPLFILWFGIGNLATILVVIYASIFPLVYSVWTGVRAVNPLWLRAATVM
                     GADEATLFRKVIWPGALPYVITGLRLSFGR"
BASE COUNT          501 a          938 c          919 g          555 t
ORIGIN      
        1 cggcatccac gtcgccaagg ttttcgccga ccttggcctc gacaaggcgc tcggcccgaa
       61 aatccagctg cgcgaccgcg ggccggacgg cctgaaggag gtcgccgccg gcgagatcga
      121 catcggcctc ggccagatca gcgagatcct tgccaacaag gacgtcgtac tggtcgggcc
      181 gctgccgcct gccatccagg gcgctgttac tttctccggc gcgatccatt cacaggcaaa
      241 ggatagggcg gcggcgaagg cgctgatcga tctgctgacc tcgccggagg cgaaagagaa
      301 attcaaggcc gtcggcttca ccgtgagctg acgggacaac aaggacatcg aggacgattc
      361 aaagggagga catcatgacc aagctgacca tgccacgacg cgattttctg accatcgccg
      421 gcggcgcact cgccgccgcc gcaaccggca tgcgtgcaaa tgccgaaggc gcccagatca
      481 atttcggcta ccagaatacg tcatggggca cgatcggcat gatcgcgggc tcggaagacc
      541 tgttcaagaa agccggtgcc aacgtcgcca tccacacctt cgacagcggc aagacgacgc
      601 gtgacgccat gatctccggc cgcatcgaca tcggcgtgat cggcgcgaca ccgttcgtga
      661 tcggcgcggc gaagggcgag ctggaggcaa tcggtcttgc cctctatggc gcgaagacgc
      721 ttgccgtcgt cgccggcctc aagtccggca tcaattcgat caaggatctc aagggcaaga
      781 aagtcggctc gcagcccggc tcggcgaccg atcacgtatt cctgaccaag atcctaccca
      841 aggcgggact gaccaaggac gacgtgcaga tggtcaacgt gcgcttccag aaccacatct
      901 cggcgctcgc ctccggctcg atcgatgctt ttgccggcgt cgagccgttc ccgtcggtgg
      961 ccgaggtcga gaagctcggc aaggtgatca ccgactacag caactatgac ctgcagccga
     1021 tcatcctcgc cgccaacaag tcggtgatcg aaaagaatag agacggcgtc gtcgccttca
     1081 tgcgcggctg gctggcggcg gtgaaaatct atcaggacaa gcgcgacctc gcgacctcga
     1141 tcgtgctcaa gcacttcaag gaccagggat tctcggtcga tgacaaggtc atcaagctga
     1201 tgctgtcgaa gatcgacgtc aatccggagt tcggcccgca gctcaagacc tatctgaccg
     1261 aggaggcgca gaccttgctc gagcagaaga agatctccgc catcccggac tggaatacgc
     1321 ggctcaacag cagcatcctg tccgcggcgc gcgccaaagc ctgatgcttg agtgtaatcc
     1381 gttggaattg gcacaacctc cgctcattcc cgcgaaagcg ggaatccggt gttttcgcct
     1441 ctgggtccca tcgcaaatcg gctttagccg acttgcgcac gtatatgcca acgtcgggta
     1501 tacccgatgt tggtgcgcgg ggacgagcgg attaccaatc gactcattcc aatcggacca
     1561 aaccttgaac gcgcgatgaa acgggcgctc tcgatcggcc tgccgctcgc gaccttcgcg
     1621 gtgatcgcgg cggcgtggca gatgctcgcc acctacggcg gctttccgcc gaagctggtg
     1681 ccgggtctcg gcaagattgc cgaggccttc gtgcggcttg ccggcgacgg cacgctgctg
     1741 gcggcgacca tcgcgaccct ctatcgcctc ggcgccggat tcctcatcgc cgccgtcatc
     1801 ggcgtcacgc tcggtatcgc gatggggcgg cggcaatgga tcgaggacac gctgctgccg
     1861 atggtgacgt ttctctatcc gatccccggc ctcgcctacg cgccgctgtt cgtgctgtgg
     1921 ttcgggctcg gcgacgtccc gaccatcctc ctcaccggcg tggcctcgag cttcaccgtc
     1981 atcatcaatg cctggaaggg cgtgaaggcg gtgaagccgg tctggctgcg ttccgccgag
     2041 gtgatggggg cgcgcggccg cgacatcttc cgccgtgtcg tgctgcccgg tgccctgccc
     2101 tacatcatga tcgggctgcg gctcggcctc gcgcaggcct ggcgcattct gatcgcagtc
     2161 gagatgctga tgtcggtaaa gcgcgggctc ggctggctga tcttcggcgc gcagaccttc
     2221 ctcaacaccg acgtgatgct cgccaccatc gccgtcattg gcgcgatcgg gatcctgctc
     2281 gaatggcagg tgttcggccg catcgaacgc tggacggtcg tgcgctgggg catggtggcg
     2341 gcatgagaaa gggattttcg tggatcgctt ttgcgcgcgg cgtggccggg atcgccgtgc
     2401 tgctcatcat ctgggagatc tttgcccgca gcggcatgtt ttcgccggcg atgacgcctc
     2461 cgcttgaatc ggtcggccgc gtcatctgga ccatgctggc cgacggcact ttcttcagga
     2521 acgcgctggc gaccctggtg cgcgtcttca tcggcctcgg catatcgttc gcggtggcgg
     2581 tcccgctcgg cgtgatgatg gcgcgttact gggtggccga acgcatcttc ctgccggtgc
     2641 tgagcgtgct gctgcccatc ccgtcgctcg cctgggtgcc gctgttcatt ctgtggttcg
     2701 gcatcggcaa cctcgccacc attctggtcg tgatctatgc ctcgattttt ccgctggttt
     2761 acagcgtctg gaccggcgtg cgcgcggtga acccgctctg gctgcgcgcg gcaaccgtca
     2821 tgggtgccga cgaggcaacg ctgttccgca aggtgatctg gccgggcgcc cttccctacg
     2881 tgatcaccgg cttgcggctc tccttcggcc gcg
//
LOCUS       DASSRL010000308         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_25571, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000308 DASSRL010000000
VERSION     DASSRL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25571"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..847)
                     /locus_tag="VFL49_06370"
     CDS             complement(77..847)
                     /locus_tag="VFL49_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET7911634.1"
                     /translation="MVRETGTFRMDECFVPSRRKVVGTTGVLALAAVLQPWRTLAQPT
                     SGQKLKIGVIGSGNIGGTIGGLWVKSGHPVLFSSRHPEELKDLVQRLGASAQAGTVAQ
                     AIAFGDVSFIAVPYGALPQIGRDFRDALKGKIMLDACNAVAARDGAVAEEVERDGIGI
                     TSQKYLAGTRLVRAFNTMSYAIFAREANRSDPKLAIPIAGDDAEALRVTEGLIRDAGF
                     DPVVVGKLADARRFQRGGPGYGQPVSAAELKQKLSLAP"
     gene            complement(953..1984)
                     /locus_tag="VFL49_06375"
     CDS             complement(953..1984)
                     /locus_tag="VFL49_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET7911635.1"
                     /translation="MLPRRRWLNVGALCAALALVAGTFALANVVLRYPAAPTRIEITA
                     TPITSFDNRDPTRTRFGDLEFRGGLELSSRHPAFGGISAIHVEPDGDRFIAATDRGSW
                     LRGRISYRDGRPAGIADAEMAPILGADGRPLAARGWFDVESLTEREGILYIGIERVQQ
                     IVRFDYRRDGLRARGQPIDVPAEFKTFTFNKSLECLAAPREGPLAGQLIAVTERSLDS
                     AGNLRSFILSGDRMARFSVKRTDDFDVSDCAVLAQGDLLLLERRFSMTRGLAMRIRRI
                     TLGSIKADVVVDGHSMIEADLGYQIDNMEGIALHRNASGEAIITLVSDDDFSVIQRNL
                     LLQFAVIGD"
     gene            complement(1984..>2903)
                     /locus_tag="VFL49_06380"
     CDS             complement(1984..>2903)
                     /locus_tag="VFL49_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HET7911636.1"
                     /translation="DLCEAEELDRLRGYLDKQLSHLQGVVARLANRLQRRLMAQQNRS
                     WEFDLEEGQLDPARLSRVIIDPLHPLSFKAEKDMQFRDTVVTLLLDNSGSMRGRPITV
                     AATCADILARTLERCGVKVEILGFTTRAWKGGQSREHWLTAGKPANPGRLNDLRHIIY
                     KSADAPWRRARKNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPV
                     DDSTLSVNPGNYLERHLRWVIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELG
                     GAMTEKLAELFEEHAAAERPSPRARRRVA"
BASE COUNT          572 a          922 c          886 g          523 t
ORIGIN      
        1 ccgaacgacc aaaacgctgc cgcacgctct tgcggcgtag ccggcagtgc ccagccgagc
       61 caacgctgca aagggttcac ggcgccaacg acagtttctg cttgagctct gcggcgctga
      121 ccggttgtcc ataaccgggt ccgccacgct ggaaccggcg cgcgtcggcc agctttccga
      181 ccacaaccgg atcgaatccg gcatcgcgaa taagtccctc cgtcacccgc aacgcttctg
      241 catcgtcacc ggcaatcggt atcgcaagct tcgggtcgga acggttcgcc tcgcgcgcga
      301 aaatcgcgta cgacatggtg ttgaacgcgc gcacaagccg cgtgccggcg agatatttct
      361 gcgacgtgat gccaattccg tcgcgctcga cttcctcggc gacggcgcca tcgcgcgcag
      421 cgacggcatt acaggcgtca agcatgatct tgccctttaa cgcatcgcgg aaatcccgac
      481 cgatctgtgg gagtgcaccg taaggcaccg cgatgaacga tacatcacca aaggcaattg
      541 cttgcgccac ggtgccggcc tgtgccgacg caccaagccg ttgcacgagg tccttgagtt
      601 cctcagggtg acgcgaggaa aacaagacgg ggtggcccga tttgacccag aggccgccga
      661 tcgtgccgcc gatattgccg gaaccaataa caccgatttt caatttttga ccggaggttg
      721 gctgcgccag cgtgcgccat ggctgcagaa ccgccgccag ggcaagtacg ccggtcgtgc
      781 caacgacctt tcgccgcgac gggacaaagc attcatccat gcggaacgtc cccgtttcac
      841 gaaccatccg tgtcaatatt ttaggagtcc taacgcctcg cgtctctcac ggttgcgtga
      901 aaggcgcagt gaccatatca cggaagagtg tggcgcgtgg aatacctgcg ccttagtccc
      961 ctatcaccgc aaactggagc aacagattac gctggatcac tgaaaaatcg tcgtcagaaa
     1021 ccagagtgat gatagcctcg cccgacgcat tacggtgcaa agcaatgcct tccatgttgt
     1081 cgatctggta gccgagatcg gcctcgatca tcgaatgtcc atcgacgacc acgtccgcct
     1141 tgatactgcc cagcgtgatg cggcggattc gcatcgcaag tccccgtgtc atggaaaagc
     1201 gccgctcgag cagcagaaga tcgccctgcg ccaggaccgc gcaatcgctc acatcaaagt
     1261 cgtcggtacg cttgacgctg aagcgcgcca tccgatcgcc gctcagaatg aacgagcgca
     1321 agttgccggc gctgtcgagg ctgcgctcgg tcaccgcgat caactggccc gccaaaggtc
     1381 cttcgcgcgg agcagcaaga cattcgagac tcttattgaa ggtaaaggtc ttgaattcgg
     1441 cgggcacgtc gatcggttgg ccgcgtgcgc gcaggccgtc gcggcggtag tcgaagcgca
     1501 cgatctgctg gacgcgttcg atgccaatgt aaaggatgcc ttcgcgctct gtaagagatt
     1561 cgacgtcaaa ccagccgcgt gcggcgagcg gccgtccgtc ggcgccgaga attggcgcca
     1621 tttcggcgtc ggcaatgcca gcgggcctgc catcgcgata gctgatgcgg ccacgcagcc
     1681 acgagccgcg gtcggtcgcc gcgataaacc ggtcgccatc cggctcgacg tggatcgctg
     1741 aaattccgcc gaaagccgga tgtctcgacg aaagttcgag tccgccgcga aactcgagat
     1801 cgccgaagcg cgtccgtgtc ggatcgcggt tatcgaatga cgtaatcggc gttgcggtga
     1861 tttcgatgcg cgtcggcgcc gcggggtagc gcagcaccac atttgccagc gcaaaggtcc
     1921 cggcaacaag cgcgagcgcg gcgcacagtg ctccgacgtt gagccagcgt cggcggggca
     1981 gcatcaagcg acgcgtctgc gcgcgcgtgg actgggccgc tcggcagcgg catgttcctc
     2041 aaacaactcg gcaagcttct cggtcatcgc gccgccaagc tcctcggcgt cgacgatggt
     2101 gacggcgcgg cgatagtagc gcgtgacgtc gtgaccgatg ccgatggcaa tcagttctac
     2161 cggtgagcgc gtctcgattt cctcgatgac ccaacgcagg tgacgctcga gatagttgcc
     2221 cggattgacc gacagtgtgg aatcgtccac cggcgcgccg tcagagatca tcatcaggat
     2281 cttgcgctgc tcggatcggc ccagcagacg cttgtgcgcc caatcgagcg cctcgccgtc
     2341 aatgttctcc ttgagcagac cctcgcgcat catcaggccg agattcttgc gggcgcggcg
     2401 ccacggcgcg tcggccgatt tgtagatgat gtgacggaga tcgttgagac ggccagggtt
     2461 ggccggcttg ccggcggtga gccagtgctc gcgcgactgc ccacctttcc aggcccgcgt
     2521 cgtgaaacca agtatctcga ccttgacgcc gcagcgctcg agcgtgcgcg caaggatgtc
     2581 ggcgcaggtt gcggcgaccg tgatcggccg gccgcgcatc gagccggaat tatcgagcag
     2641 aagcgtcacg acggtatcgc ggaattgcat gtccttttcc gccttgaacg acagcggatg
     2701 cagcgggtcg atgatcacgc gcgacagacg cgccggatcg agctgacctt cctcgagatc
     2761 gaattcccac gaacggttct gttgcgccat cagccggcgc tgcagccggt tggcaagccg
     2821 cgccaccacg ccctgcagat gtgacagctg cttgtcgaga taaccccgca gtcgatcaag
     2881 ctcttccgct tcacagagat cct
//
LOCUS       DASSRL010000309         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_200714, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000309 DASSRL010000000
VERSION     DASSRL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200714"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1290
                     /locus_tag="VFL49_06385"
     CDS             172..1290
                     /locus_tag="VFL49_06385"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HET7911637.1"
                     /translation="MRTRAVIITSLLLAAVPLDSPAQAQLSPQGIVNGMTRPLRQMLG
                     HFGHFPRGHRHRSSNAARAAASPDRDTQGAVRSRLGRAGPAAWVVAYEEVLGFTFWPD
                     DYGPQLRSRGFDVIADTITGRFDRMRAPTRVATTGAARSDSGNDVAADQCSDPSSADD
                     KWPASRIEQALQLSDAQHATLDKLQLAANNAVKTIKSHCHQMGDLSPPDRLRALVQTL
                     WVVRDGGIAMRAPLKDFYESLTSAQKNSFAVQQPQNAPPPDDRAKGENKQYHACAAQN
                     IGSAERLVKEIEMKVKPDKAQGASLENLHKASSDMAKLLIASCAQPIPADPLGRLDSA
                     SDQITAMNYAASNVQIALDDFYGRLSQTQKARFEATGR"
     gene            complement(1294..>2938)
                     /gene="shc"
                     /locus_tag="VFL49_06390"
     CDS             complement(1294..>2938)
                     /gene="shc"
                     /locus_tag="VFL49_06390"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET7911638.1"
                     /translation="KMAGESPDAPHMRRAREAMLARGGAVHSNVFTRALLSLYGILDW
                     RSVPEMPVEIVLLPRWFPFHLSKVSYWARTVLVPLLVLQALRPRAKNALGVRIDELFV
                     EPPASIGPAPKAPHQRWIWFLLFRGIDIVLRPAVKLFPESLRRRAIDRAVEFVTERLN
                     GKDGLGAIFPAMANATLMYDVLGDTANAAIARDSIDRLLVIHDHEAYCQPCVSPVWDT
                     ALTCHALLEMGGDDAQASALRGLDWLVPNQVLDVVGDWAARRPHVRPGGWAFQYANPH
                     YPDLDDTAVVVMAMDRARRSGAGDRYDQAIARAVEWVRGLQSTNGGWAAFDVDNVHDY
                     LNNIPFADHGALIDPPTEDVTARCLSMFAQLGETPETSESVARGVQYLLRTQLKDGSW
                     YGRWGLNYIYGTWSSLCALNACGVDHNSPEIRKAVDWLLSIQNPDGGWGEDGTSYRLD
                     YRGHEPAASTSSQTAWALLGLMACGQVRNPAIARGVQYLAQTQTDSGFWNEERFTATG
                     FPRVFYLRYHGYRKFFPLWAMARYRNLTESNSVSVPFGM"
BASE COUNT          596 a          946 c          925 g          471 t
ORIGIN      
        1 cggcaagcga agaaccggcc cgagctcgcc aaagctggat aatcggcggg aaaaaaggcg
       61 ggataaccgc acttttgatc cgtccggatg gaacttccgg atcgatttgc ctgttgtgta
      121 aagcgaaaag tttcgccacc gtcaggtccg taatggccct ggagcacggc catgcgcacg
      181 cgcgccgtca tcatcacgag tttgctgttg gcagccgttc cgctggacag cccggcgcag
      241 gcgcaactat cgccgcaggg aattgtcaac ggcatgacgc ggcccctccg ccagatgctc
      301 gggcattttg gccactttcc gcgcggccac aggcaccggt cctccaatgc agcgcgggcc
      361 gccgcgtcgc ccgatcgtga cacgcagggc gccgtcagat cgcgtttagg tcgcgcagga
      421 cccgcggcat gggttgtggc ttacgaagag gtgctgggtt ttacgttctg gccggacgac
      481 tacggcccgc aactccgcag ccgcggcttt gacgtgatcg cagacacgat caccggccgt
      541 tttgaccgga tgcgggcgcc gacgcgggtg gcgaccaccg gagcagccag gagcgacagc
      601 ggcaatgacg tagcggccga tcaatgcagc gatccgtcaa gcgccgatga caagtggccg
      661 gccagcagaa tcgagcaagc cttgcagctg tccgacgccc agcacgcgac gctcgataag
      721 ctgcagcttg ccgcaaacaa tgcggtcaaa acgatcaaat cgcattgtca ccagatggga
      781 gacctttcgc cgcctgaccg gctgagggcg ctggtgcaga cactgtgggt cgtgcgcgac
      841 ggcggcatcg ccatgcgggc gccgctcaaa gacttttatg agagcctgac gagcgcgcag
      901 aagaacagct ttgccgtcca gcagccgcag aacgctccgc cgcccgacga ccgggcgaaa
      961 ggcgagaaca agcaatatca cgcctgcgcc gcgcaaaata tcggcagtgc cgaacggctg
     1021 gtcaaagaaa tcgaaatgaa ggtgaagccg gacaaagccc agggcgccag cctcgagaac
     1081 ctgcacaagg cctcatccga tatggccaag cttctgatcg cgtcgtgcgc acagccgatc
     1141 cccgccgatc cgctggggcg gctggattcc gcaagcgacc agatcacggc gatgaactac
     1201 gctgcaagca atgtgcaaat tgcactggac gatttttatg gcaggctgtc gcagacgcag
     1261 aaggcgcggt tcgaggcgac cggccgctga cggtcacatg ccgaacggca cgctcacgct
     1321 gttgctctcc gtcagattgc gatagcgcgc catcgcccag agcgggaaga atttccggta
     1381 tccgtgatag cgcaggtaga agacccgcgg aaatccggtc gccgtaaatc gctcttcatt
     1441 ccagaaaccg gaatcggtct gcgtctgcgc cagatattgc acgccgcggg caatcgccgg
     1501 gttgcgaacc tggccgcagg ccatcaggcc gagcagcgcc caggccgtct gcgaggaagt
     1561 gctcgccgcc ggctcgtggc cgcgatagtc gagcctgtag ctcgtgccgt cttcgcccca
     1621 acccccgtcc ggattctgga tcgacagcaa ccaatcgacg gccttgcgga tttcgggaga
     1681 attgtggtcg acgccgcagg cgttgagcgc gcagagcgaa gaccaggtgc cgtaaatgta
     1741 attcagaccc cagcggccgt accagctgcc gtccttcagc tgcgtgcgga gcagatactg
     1801 gacgccgcgc gccactgatt cacttgtctc gggtgtctcg ccgagctgtg cgaacatcga
     1861 caggcagcgc gcggtcacgt cttccgtcgg cgggtcgatc aacgcgccgt gatcggcgaa
     1921 cgggatgttg ttgagataat cgtggacgtt gtcgacatcg aacgccgccc agccgccatt
     1981 ggtgctctgt aatcccctca cccactcgac cgcgcgcgca atggcctgat cgtaacgatc
     2041 tccggcgcct gaccggcggg cacgatccat cgccatgacg acgacggcgg tgtcgtcgag
     2101 gtcgggataa tgcggattgg catactggaa cgcccagccg cctggccgga cgtgcgggcg
     2161 gcgcgccgcc cagtccccga cgacatcgag cacctggttc ggcaccagcc aatcgagccc
     2221 gcgcaaggcg cttgcctgcg cgtcgtcacc gcccatttcg agcagcgcat gacatgtcag
     2281 cgccgtatcc cacaccggcg acacgcaagg ctggcagtag gcctcgtgat cgtgaatgac
     2341 cagcagcctg tcgatcgagt cgcgcgcaat cgccgcattg gccgtgtcgc caagcacgtc
     2401 atacatcagc gtcgcattcg ccattgcggg aaagatcgcg ccaagaccgt ctttgccgtt
     2461 aagacgttcg gtgacgaatt cgaccgctcg atcgatggca cgccggcgca ggctttcggg
     2521 aaaaagcttt acggccggcc gaagcacgat atcgataccg cgaaacagga ggaaccagat
     2581 ccagcgttga tgcggcgctt tcggtgccgg cccgatcgat gccggcggct cgacaaaaag
     2641 ctcgtcaatg cgcacgccga gcgcattctt cgcgcgcggg cgcagcgcct gcagcaccag
     2701 cagcggcacc agaacagtcc gcgcccaata ggacaccttg gacaaatgga agggaaacca
     2761 ccgtggcagc agcacgatct cgaccggcat ctccggaacg ctgcgccagt cgaggatgcc
     2821 gtagagcgac agcagagcgc gcgtgaagac gttggaatga acggcgccac cgcgcgccag
     2881 catcgcctcg cgcgcacgcc gcatgtgcgg ggcatcgggg ctttcgccgg ccatcttc
//
LOCUS       DASSRL010000310         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1355592, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000310 DASSRL010000000
VERSION     DASSRL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355592"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFL49_06395"
     CDS             complement(<1..525)
                     /locus_tag="VFL49_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HET7911639.1"
                     /translation="MNTTALLRTLIVLMSTCLYAVSASAQQKKTAASPTDQAVLLGQF
                     GDWGAYRASPGGKKICFALSKPTAATTEPAGRNRDASYAFVSTRPSEKVKNEVSVIVG
                     YPQKPGHDANAAIGSATYAMYTQNDGAWVKNAAEEAQMVDAMRKGADLVIKSESSRGT
                     KTTDTYSLKGIAQAL"
     gene            692..1666
                     /locus_tag="VFL49_06400"
     CDS             692..1666
                     /locus_tag="VFL49_06400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET7911640.1"
                     /translation="MKALLCTRHGTPDDLELVDIPDPVAAAGEAVVRISAAALNFFDT
                     LIIANKYQTKPPLPFSPAAEFAGTVESVGASVTAVKTGDRVIGCLTHGAAREKIAAPA
                     GKLIKIPDALDFDRAAGVCVTYGTTLHALKDRARLRAGETLAVLGASGGAGLAAVELG
                     KLMNARVIACASSDEKLAFAKKHGADDAINYRKNDLKEALRRATSGRGADVIYDPVGG
                     PYAEPALRSIAWQGRFLVIGFAAGDIPKLPLNLVLLKGCDVVGVFWGSWMDRDPQGHR
                     ANTEQLLAWCVEGKLSSHVHAIYPLADGPTALKAIAARQVMGKVILRP"
     gene            complement(1684..2043)
                     /locus_tag="VFL49_06405"
     CDS             complement(1684..2043)
                     /locus_tag="VFL49_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET7911641.1"
                     /translation="MAFSYSAQSAFWRRKDDDTAFRRQFWQKLRNVAASIPFAEDLLA
                     AYYCAFDRQTPVSVKAALFGALAYFVMPADAIPDVVPVLGFTDDAAMLAAAIKLVSDH
                     IRPEHRDAARKSLADAK"
     gene            complement(2086..2379)
                     /locus_tag="VFL49_06410"
     CDS             complement(2086..2379)
                     /locus_tag="VFL49_06410"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7911642.1"
                     /translation="MSAKLTGDARKSALAGLKGWSEATGRDAITKKFVFRDFSQAFGF
                     MARAALVAEKMDHHPEWFNVYKTVDVTLSTHDAGGLTDRDVKLAEAMDRIAGQ"
     gene            2498..>2915
                     /locus_tag="VFL49_06415"
     CDS             2498..>2915
                     /locus_tag="VFL49_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017968795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7911643.1"
                     /translation="MSWSLNIGRVAGTVVRVHLTFLLFLAWIFAASYASGGAATAWDS
                     LAFMILLFVCVLLHEFGHIFTARAFGVPTPYVTLLPIGGVAQLERIPEEPWEEFLIAI
                     AGPLVNVVIAGVLVYIFGAELHTHAAAAVDNVGIPLV"
BASE COUNT          532 a          891 c          884 g          608 t
ORIGIN      
        1 gagtgcctgc gcgatgccct tgagggagta ggtatccgtg gtcttcgtgc cgcgggacga
       61 ctcactcttg atcaccaggt cggcgccctt gcgcatggca tccaccattt gcgcttcttc
      121 cgccgcattc ttcacccagg cgccgtcgtt ctgcgtgtac atggcgtagg ttgccgagcc
      181 gatcgccgca tttgcatcgt ggcccggttt ttgcggatag ccgacgatca ccgacacctc
      241 gttctttacc ttctccgacg gccgcgtcga cacgaaagca tatgaggcgt cacggttgcg
      301 gccggccggt tccgtggtgg ccgctgtcgg cttcgaaagc gcgaaacaga ttttcttgcc
      361 cccgggcgac gccctgtacg ccccccaatc gccgaattga ccaaggagaa cagcctgatc
      421 agtcgggctt gcggcggttt tcttttgctg ggcggaggct gatacggcgt acaggcaagt
      481 cgacatcaga acaatgagcg tgcgaagcaa tgcggtcgta ttcatcgggc cctaccggtg
      541 acgatattga cggttgttga gtcgccccca taaagaatcg ctggatatta gcccattacc
      601 gacgaatgtc ggcaatgtga aggcggcaat ccgccgctcg tgaccatggt agatatcgtc
      661 gcctctccga cgcttcacca agtggaaggc catgaaagca ctcctctgca cccgccacgg
      721 aacacctgac gatctcgaac ttgtcgacat tcccgaccct gtcgcggcag ccggcgaagc
      781 cgttgtgcgt atcagcgcgg ccgcattgaa tttcttcgat acgctgatca tcgccaacaa
      841 gtaccagacc aagccgcccc tgccgttctc gccggcagcg gaatttgccg gaacagtgga
      901 gagcgtggga gcgagcgtga cggcggtgaa gactggcgat cgcgtcattg gctgccttac
      961 ccacggtgcc gcacgtgaaa aaatagcggc gcctgccggc aagctcatca agatacccga
     1021 tgcgcttgat ttcgatcggg cggccggagt ttgcgtcacc tatggcacga cgcttcatgc
     1081 gctgaaggat cgtgccaggc tacgcgcggg cgaaacgctg gctgtgctcg gcgcctccgg
     1141 cggcgcgggc ttggcggcgg tcgaactcgg caagctgatg aatgcacgcg tgatcgcgtg
     1201 cgcatcctcg gacgaaaagc ttgcgttcgc gaaaaagcac ggcgccgatg acgcaatcaa
     1261 ttaccgcaag aatgatctta aggaggcgct tcgtcgcgcc acaagcggcc ggggtgccga
     1321 cgtcatctat gatcctgtcg gtggacctta cgccgagccg gcattgcgtt cgattgcgtg
     1381 gcagggccgt tttcttgtga tcggcttcgc cgccggcgac attccgaaac tgccgctcaa
     1441 cctcgtgctg ctcaaaggct gcgacgtcgt cggcgtgttc tggggctcat ggatggaccg
     1501 tgacccgcag ggtcatcgcg cgaataccga gcagttgctg gcctggtgcg tggaggggaa
     1561 gctgtcatcg cacgttcatg caatctatcc gctggccgac ggccctacgg ccctaaaggc
     1621 cattgcagca aggcaagtga tgggaaaagt gatcttgcga ccgtaatctg tcgcgaaagg
     1681 gcgttacttg gcgtccgcaa gactttttcg tgccgcgtcg cgatgctccg ggcgaatatg
     1741 atcggatacc agcttgattg cggcggccag catcgctgca tcgtcggtga agcccagaac
     1801 gggcaccaca tcgggaatcg cgtcggccgg catcacgaaa taagcaagcg cgccgaaaag
     1861 ggccgccttc accgacaccg gcgtttggcg atcgaaggcg caataatagg cggcgagcaa
     1921 atcctcggcg aatggaatcg acgccgctac atttcgcagc ttctgccaga actggcgccg
     1981 gaacgcggta tcatcgtctt tgcgccgcca gaacgcggac tgcgcggagt atgagaatgc
     2041 catggccgat tatgtggttg ttaaaacggg cgcccgcaag gcgggctact gcccggcaat
     2101 tctgtccatg gcctcggcaa gcttcacatc gcggtcggtc aggccgccgg catcgtgcgt
     2161 ggagagggtt acgtccaccg ttttgtagac attgaaccac tccggatggt ggtccatttt
     2221 ttccgcgacc agtgccgcgc gcgccatgaa gccgaaggcc tggctgaagt ccctgaacac
     2281 gaatttcttc gtaatcgcgt cgcggccggt cgcctccgac cagcccttga gtccggccag
     2341 cgctgattta cgggcatctc cggtaagttt ggcgctcatg atcgagcctc tctttcctgc
     2401 tttactgtcc gccggctgtg ggtcgacgct gcgccctcgc attgtgctcc tatcgatatt
     2461 aactcatgaa catagatacc gaccatccga ggctctcatg tcctggtctc tgaatatcgg
     2521 cagagtggcc ggcaccgtcg tccgcgtcca cctcaccttc ctgctgtttc tcgcctggat
     2581 tttcgcggcg agctacgcat cgggcggcgc cgccacagcc tgggacagcc tcgccttcat
     2641 gattctgctg ttcgtctgcg tgctgctgca cgaattcgga cacattttca ccgcccgcgc
     2701 cttcggcgtg cccacgccct acgtgaccct gctgccgatc ggcggcgtgg cacagctcga
     2761 gcgcattccg gaagagccgt gggaagagtt cctcatcgcg atcgcggggc cgctggtcaa
     2821 cgtcgtcatt gcaggtgtgc tggtttacat ttttggtgcc gaactgcata cgcacgcggc
     2881 ggcggcggta gacaacgtag gaattccgct cgtcg
//
LOCUS       DASSRL010000311         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_411130, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000311 DASSRL010000000
VERSION     DASSRL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411130"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1430
                     /gene="hemA"
                     /locus_tag="VFL49_06420"
     CDS             201..1430
                     /gene="hemA"
                     /locus_tag="VFL49_06420"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HET7911644.1"
                     /translation="MDYKDYFASALGKLRNERRYRVFADLERVAGRFPHAIWHSPKGQ
                     RDIVIWCSNDYLGMAQHPKVIGAMVEAATRMGTGAGGTRNIAGTNHPLVELERELADL
                     HGKPAALVFTSGYVSNETGISTIAKLLPNCLILSDALNHNSMIEGVRQSGSEKKIWRH
                     NDLQHLEELLRTADHARPKLIVFESLYSMDGDVAPVHAICDLAARYNAMTYCDEVHAV
                     GMYGPRGGGICDRDNAMGRVDVIEGTLAKAFGCLGGYIATSEDVVDAVRSYAPGFIFT
                     TALPPAICAAATAAIRHLKSSSWERERHQERAARVKAVLNAAGLPVMPSTTHIVPVLV
                     GDPEKCKAASDLLLAEHGVYIQPINYPTVPRGMERLRITPSPYHDDALVDALAEALVD
                     VWDRLGLRRGEQAAAAE"
     gene            complement(1444..>2432)
                     /locus_tag="VFL49_06425"
     CDS             complement(1444..>2432)
                     /locus_tag="VFL49_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET7911645.1"
                     /translation="ILAVAGVAIAGRPLLIPTPLMRAIFVLIGTSLGAVVTPETLHGM
                     ATYPLSIAILVVAMSVISFGGAGYLRLVHRWGTVDSYLAAAPGGMSQVLALGAELGGD
                     LRAIAIVQSIRVVVIAVGLPAGLSLLGLVGHAPSRVTGPLSVDVLDELAILLAASTVV
                     AIIAYRIRFPGGLLFGAMLTSAILHGSGVIHAVLPWWVANAAMIAMGAITGSRFANTS
                     PRMLLNFAAAAFGSFAVAVAISAVFAAVLISMLSLRAAEVMIAFAPGAVDAMMLLALA
                     LSLDPVYVGAHHLTRIFFVSLTMPLLARYSARTQMTVRDAAKRPVKPPPFQD"
BASE COUNT          498 a          789 c          760 g          385 t
ORIGIN      
        1 ggattttaca ttgcaactgc atgtcggcgc gctgctgctc gcggccttgc gaggacatct
       61 cgacattgca gcattccgtt agactgaaac cggacacgac cagcgatctc caactttgga
      121 atgcgacgaa tctcgcctcg acaaataccc tggaaccctt ctaaaagtag gactgcgcgg
      181 ggcgccagaa agaaaaagat atggattaca aagattattt cgcatctgcc ttgggcaaac
      241 tgcggaacga acggcgttac cgggtctttg ccgatcttga acgcgtcgca ggccgttttc
      301 cgcacgcgat ctggcactca ccgaaaggtc agcgggacat cgtgatctgg tgctcgaacg
      361 attacctcgg catggcgcag cacccgaagg tgatcggcgc catggtcgag gccgcaaccc
      421 gcatgggcac cggcgcgggc ggcacgcgca acatcgccgg caccaaccat ccgctggtcg
      481 aactcgagcg cgagctcgcc gacctgcacg gcaagccggc cgcactggtg ttcacgtcag
      541 gctatgtgtc gaacgagacg ggcatctcca caatcgcgaa gctgttgccg aattgcctga
      601 tcctttccga cgcgctcaat cataattcca tgattgaagg cgtccggcag tcgggaagcg
      661 aaaagaaaat ctggcgccat aatgatctgc agcatctcga ggagcttctc aggacggccg
      721 accacgcccg cccgaagctc atcgtcttcg agagcctgta ctccatggac ggcgacgtcg
      781 cgccggtgca tgcgatctgc gatctcgccg cgcgctacaa cgcgatgacc tattgtgatg
      841 aagtgcacgc ggtcggcatg tacggaccgc gcggcggcgg catctgcgac cgcgataatg
      901 ccatggggcg cgtggacgtg atcgagggca cgcttgccaa agccttcggc tgcctcggcg
      961 gttacattgc gaccagcgag gacgtcgttg atgcggtgcg ttcctatgca ccgggcttca
     1021 tctttaccac ggcgctgcca cccgcgatct gcgccgccgc gaccgccgcg atccgccacc
     1081 tcaagtcgtc gagctgggag cgcgagcggc atcaggagcg cgccgcgcgc gtgaaagccg
     1141 tgctcaatgc ggcaggcctg ccggtgatgc cgagcacgac acatatcgtt cccgttctcg
     1201 tcggcgatcc cgagaagtgc aaggccgcaa gcgatctgtt gctggccgag catggcgtct
     1261 atatccagcc gatcaactat ccgaccgtgc cgcgcggcat ggagcggctg cgcatcacgc
     1321 cttcgccgta ccatgacgac gcgctggtcg atgcgcttgc cgaagcgcta gtcgacgtct
     1381 gggaccggct cggattgcgc cggggcgagc aggctgctgc ggccgaatag tccgccggcc
     1441 ccgtcaatcc tgaaatggcg gcggctttac aggccgtttt gcggcatcac gcaccgtcat
     1501 ttgcgtgcgc gcgctatacc gtgccagcag cggcatggtc agcgacacga agaagatgcg
     1561 cgtgaggtgg tgcgcgccga cgtagacggg gtcgaggctg agcgccagcg ccagcagcat
     1621 catggcatcg acggcgcccg gcgcgaaggc gatcatcacc tcggcagcgc gcagcgacaa
     1681 catgctgatg agcaccgccg cgaaaaccgc cgagatcgcg accgcaaccg cgaacgatcc
     1741 gaaggcggcg gcagcgaaat tcagcagcat ccgcggcgag gtattggcaa agcgcgatcc
     1801 ggtgatcgcg cccatcgcga tcatcgcggc gttcgcaacc caccagggca ggactgcgtg
     1861 aatgacgccg cttccgtgca ggatcgccga ggtcagcatg gcgccgaaga gcaacccgcc
     1921 cggaaagcgg atgcggtaag caatgatcgc aacgacggtc gacgctgcga gcaggatagc
     1981 cagctcgtcg agcacgtcaa cactgagcgg gccggttact cttgacggtg catggccgac
     2041 gagtccgagc agcgacagtc cggccggaag tccgacagca atcaccacga cccgaatgct
     2101 ctgcacgatc gcgatcgccc gcagatcgcc accgagctcg gcaccgagcg ccagcacctg
     2161 cgacatgccg cccggtgccg ccgccaaata ggaatcgacg gtgccccacc ggtgcacaag
     2221 acgcagatat cccgcgccgc cgaaggaaat gaccgacatc gcgacgacca ggatggcaat
     2281 gctcagcgga taggttgcca tcccgtgcaa ggtttcgggt gtcaccaccg cgccaagcga
     2341 cgtgccgatc aatacgaaaa tcgcccgcat cagcggcgtc gggatcagca gcggccggcc
     2401 ggcgatcgcc acccctgcca ccgccaggat cg
//
LOCUS       DASSRL010000312         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_760843, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000312 DASSRL010000000
VERSION     DASSRL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760843"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFL49_06430"
     CDS             <1..974
                     /locus_tag="VFL49_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7911646.1"
                     /translation="KDANERSSRNAVDTIIANMERWRWLPRDLGEAHVIVNVPDFSLG
                     VYKDDALVWHTKIVVGKPGRLATPLLSETMKYLTVNPTWNVPPSIIRNEYLPALERDP
                     DALERIGLRVAYNQDGSLRVYQPPGERNALGRIRFNFPNQFLVYQHDTPDKKLFARDS
                     RAFSHGCMRVQDPEKYAEILLSLSQPEEGFTIARIRSLYGEQEHTINLRQPIPVHVTY
                     QTAFVDEKGQLNFRRDIYGLDAAVLKLMRNDERMIADTPVARPRNASSKPVMARLPER
                     EYGEWPGQYQYRSGGWFGFGAADRSYASSPYRSRSPYRGFDRADGIW"
     gene            1329..1460
                     /locus_tag="VFL49_06435"
     CDS             1329..1460
                     /locus_tag="VFL49_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911647.1"
                     /translation="MVETVATSHIVGVLLSEQVQQRTIRIGRFSANDRGAKGSKYPK"
     gene            complement(1617..2060)
                     /locus_tag="VFL49_06440"
     CDS             complement(1617..2060)
                     /locus_tag="VFL49_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7911648.1"
                     /translation="MPNTVRLHRVLATKPDKVYRAFVETDAVAKWLPPNGFACTVHHL
                     DAKVGGTHKMSFRNFTTGMSHSFGGKYLELVPGERLRYTDKFDDPNLPGEMQVTVTLR
                     QVSVGTELNIVQEGIPDAIPAEACYLGWQESLKNLARLVEPEINR"
BASE COUNT          447 a          687 c          652 g          408 t
ORIGIN      
        1 gcaaggacgc gaacgagcga agcagcagga atgccgtcga cacgatcatt gccaacatgg
       61 aacgctggcg ctggctgccg cgcgatcttg gcgaagcgca tgtgatcgtg aacgtccctg
      121 atttctcgct cggcgtctac aaggacgacg cgcttgtctg gcacaccaaa atcgtggtcg
      181 gaaagcccgg caggctggcg accccgctgc tcagcgagac gatgaagtat ctcaccgtca
      241 atccgacctg gaacgtgccg ccctcgatca tccgtaacga atatctgcct gcactcgaac
      301 gcgatccgga cgcgctggag cggatcggac tgagagtcgc ctataaccag gacggctcgc
      361 ttcgcgtcta ccagccgccc ggcgagcgca atgcgctggg acggattcgc ttcaacttcc
      421 cgaaccagtt cctcgtgtac cagcacgaca cgccggacaa gaaattgttc gcgcgcgaca
      481 gccgcgcctt cagccacggc tgcatgcgcg tgcaggatcc ggaaaaatat gccgagatcc
      541 tgctctcatt gtcgcagccg gaagaaggct tcaccatcgc ccgcatcaga agcctctatg
      601 gcgagcagga gcacacgatc aacctgcgcc agccgattcc ggtgcacgtc acgtatcaga
      661 ccgccttcgt cgacgaaaag gggcagctca atttccggcg cgacatttac ggtcttgacg
      721 ccgcggtcct gaagctgatg cgcaacgacg agcgcatgat cgcggatacg ccggtcgcac
      781 ggccgcgcaa cgccagcagc aagccggtga tggcgcgcct gccagaacgc gaatatggcg
      841 aatggccggg acagtaccag tatcggagtg gcgggtggtt tggttttggc gccgccgacc
      901 gatcctacgc atcctcgccg tatcgctctc gctctccgta tcgtggcttc gatcgcgctg
      961 acgggatctg gtgatgcacg tcttgcgtgc aaacagcgta gcaccatcta cggtgcgttg
     1021 cttccgtcga cagagaagtc ccggcccaca acgtcatcgt gagcgctggc tctgcggacg
     1081 gttcgagcca tcctgacgcc ggcatgccgg ataacagcag gactattgcc gccgatctcc
     1141 atcgcccaat acctccggcc ggtagccgac accgcggatg caacggcctg gcacccgccg
     1201 agcattatgt ttcggcggtg cgccggcgac tgcatccaca ttcgtcttgc gcaagcttcg
     1261 gtctcacatc ccaccgcgac attctcggca acggcaccct tcggtccccg atgccgcatg
     1321 aaacctgaat ggtcgagact gttgcgacga gccatatcgt tggcgtgctg ctgagcgagc
     1381 aagtgcagca acgaacaatc cgcataggcc gattcagcgc aaacgatcgt ggcgcaaagg
     1441 gcagcaagta tccgaagtga cgtcaccgcc ccacatctcc tgagcgcaag accatagcct
     1501 agcggagttt caacgcctcc gggaggagct ggtgaaattg tgcaccaacc tgtccaattc
     1561 gatgcgcgcg gaaccgtagg cgtttttttc ttcagccgac gcgcctgcgc agcgtactac
     1621 cgattgattt ccggctcgac aaggcgggcg agatttttca gcgactcctg ccagccaaga
     1681 tagcaggctt ccgccgggat ggcgtcgggt atgccttcct gcacgatgtt gagctcggta
     1741 ccgaccgata cctgcctgag cgtcaccgtc acctgcatct cgcccggcaa gttgggatcg
     1801 tcgaatttgt ccgtgtagcg caagcgctcg cccggcacga gctccagata tttgccgccg
     1861 aacgaatgac tcatgccggt ggtgaagttt cggaacgaca tcttgtgggt gccgccgacc
     1921 ttcgcgtcga ggtgatgcac agtgcaggca aagccgttgg gcggcagcca tttcgccacg
     1981 gcatccgtct cgacgaaggc gcgataaacc ttgtcgggct ttgtcgccag gacgcggtgc
     2041 aatcgaacgg tattgggcat ggatgtttct cccaggaggc gcggcgagtt atcggatacg
     2101 gatctgctgc actataaaga aggcgataag actacggcgc ctcaacccca aggtaggccg
     2161 ccagcacgtt tggatcgttg agaacgcgcg cggg
//
LOCUS       DASSRL010000313         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1530851, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000313 DASSRL010000000
VERSION     DASSRL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530851"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFL49_06445"
     CDS             <1..759
                     /locus_tag="VFL49_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7911649.1"
                     /translation="DPHNRTGPQTSRNYAWRDYGNRVGNWRFFEILDELKLPASILLN
                     SAVCYEYPDIVEKIKQRGDDVLGHGRTNAETLRGMWEDDERRVIQECVDVLTKHVGVR
                     PTGWMGSGAAETSVTPDLLKECGFTHLLDWPVDDQPIWMRTRSGPLLSVPYPMELNDA
                     GTLALRDHTGRQFADMIVDQFEELLEASEKQPLVFALSLHGFILGQPFRLRPLRQALK
                     HCVQHKHADQIWFTRAGEISKYCYSMEPGILPGG"
     gene            768..1736
                     /locus_tag="VFL49_06450"
     CDS             768..1736
                     /locus_tag="VFL49_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET7911650.1"
                     /translation="MNVSATANATTRLLKATPDTVHWGYLDAAIKPALTIDSGERVTI
                     ECATCNPEWMPAKDSGFDILPDIVEIHQKVKKGSGNHIFTGPVYVRGAAVGDVLEVKI
                     LDIKVRQNWGWNLFRGYMGTIPEDFPYTRIIHVSLDLKANVATTASGYKIPMAPFFGQ
                     LAVAPAAVFGRQNSKEPREFGGNLDCKELTAGSTIYLPVWNEGALFSTGDGHAAQGDG
                     EVDGTAIETALTGTFEFNVKKKLGWKMPRAETPTHYITFGLDTDLDDAARQALKEMID
                     WIVSMTGIHKDEAYAMCSFAGDLHVTQTVNNVKGVHAMLQKSILKK"
     gene            1897..2202
                     /locus_tag="VFL49_06455"
     CDS             1897..2202
                     /locus_tag="VFL49_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911651.1"
                     /translation="MDGFDFLPAARSSGVDDLLTCIRSRDALARLGEVYDYILVDLPP
                     MLESGETATMMTVAGGCVLVTHSDCSSTDDVARALELSLLEPEQITAAALITSSPVQ"
     gene            complement(2215..3735)
                     /locus_tag="VFL49_06460"
     CDS             complement(2215..3735)
                     /locus_tag="VFL49_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HET7911652.1"
                     /translation="MSPFILRGFALALVLASALHPANADDAAKNRAQPAVEQGAERHV
                     AAKGVLRLLPPDAISDHSIDTPRGKLAYSATAGTLAFYDQSGEQSASVFYTAYVAKNG
                     AANRPLTFAFNGGPGAASAFLHLGLVGPRILDFGSDPHDATRAVLRDNPDTWLAFTDL
                     VMIDPIGTGWSRAVKSEDSKHFWGIRSDADAMAKAIALYVAKNNRTGSPKYLFGESYG
                     GFRAAKTARALQRDQGITINGIVMLSPLLEGWLTFGDDGSALHAALQLPSLAAAELER
                     RHAFTAEALAAAEKFAMTDYLTTLAGAPPKDDTARTFYSRVAQMTGLPPDAITQARGF
                     AARAYVKNLRASEGKVVSRYDAAFAVDDPYPESRSSRGPDPILDAITRSYGGAMAAYA
                     RNELGFKTEMTYALLAGDVTSHWDWQGGRVQASAEDDLRVLLAFGPSFRLLIAHGYSD
                     MITPYAMTRYVLDHMPVFDPPGRVQLKLYRGGHMLYLDPKSRRAFSADAAAFFRTE"
     gene            complement(3786..4061)
                     /gene="infA"
                     /locus_tag="VFL49_06465"
     CDS             complement(3786..4061)
                     /gene="infA"
                     /locus_tag="VFL49_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7911653.1"
                     /translation="MAKEELLEFDGTVTEVLPDGNFRVKLDNDHEVLAYTAGKMRKFR
                     IRTGVGDRVIVEMSPYDLQRGRISFRHKGDAPSPAPQQRRPNFRGRR"
BASE COUNT          825 a         1260 c         1303 g          788 t
ORIGIN      
        1 gatccgcaca accgcaccgg tccacagacc tcgcgcaact acgcgtggcg cgactacggc
       61 aaccgcgtcg gcaattggcg ctttttcgaa attctcgacg agctcaaact gcccgcgtcg
      121 atcctgctga attcggccgt ctgctacgaa tatccggaca tcgtggaaaa gatcaagcag
      181 cgcggcgacg acgtgctcgg ccacggccgc accaatgccg agacgctgcg cggcatgtgg
      241 gaggacgacg agcgccgcgt catccaggaa tgtgtcgatg tcctcacgaa gcatgtcggc
      301 gtgcggccga ccggatggat gggatcgggc gccgccgaga cgagcgttac gcctgatctc
      361 ctcaaggaat gcggcttcac gcatctgctc gattggccgg tcgacgatca gccgatctgg
      421 atgcgcacgc gttcgggtcc gctgctgtcg gtgccctatc cgatggaact caacgatgcg
      481 ggcacgctgg cgttgcggga ccacaccgga cgtcagttcg ccgacatgat cgtcgatcag
      541 ttcgaagagc tgctcgaagc gtcggaaaag cagccgctcg tctttgcgct gtcgctgcac
      601 ggcttcattc tcggacagcc gttccgcctg cgcccgctgc ggcaggcact caagcattgc
      661 gttcagcaca agcacgctga ccagatctgg ttcacacggg ccggcgagat ctcgaagtac
      721 tgttacagca tggagccggg catcctccct ggcggttgaa gcttgccatg aatgtgagcg
      781 ccacagcgaa tgcgacgacc aggcttctga aagcaacgcc ggacacggtg cattggggct
      841 atctcgacgc cgccatcaag cccgcgctca ccatcgattc cggcgagcgg gtaacgatcg
      901 agtgcgctac ctgcaatccc gaatggatgc cggcgaagga cagcggcttc gacattctgc
      961 cggacatcgt cgagattcat cagaaggtga agaaaggatc cggcaatcac attttcaccg
     1021 ggcccgtcta tgtccgcggt gccgcggtcg gagatgtgct cgaagtgaaa attctcgata
     1081 tcaaggtccg gcaaaattgg ggctggaacc tctttcgcgg ctacatgggc acgatcccgg
     1141 aagactttcc gtatacgagg atcattcacg tttcgctcga tctgaaagcc aatgttgcga
     1201 ccacggcttc cggctacaaa atcccgatgg cgcccttctt tggccagctt gcggtggcgc
     1261 cggccgcagt gttcggacgg cagaacagca aggagccgcg cgaattcggc ggcaatcttg
     1321 actgcaagga gctgacagcg ggcagcacga tttatcttcc ggtatggaac gaaggcgcgc
     1381 tgttctcgac cggcgacggc cacgccgcgc agggtgatgg cgaagtcgac ggaaccgcta
     1441 tcgagaccgc gctcaccgga acgttcgaat tcaacgtgaa aaagaaactc ggctggaaga
     1501 tgccgcgcgc ggaaactccg acgcactaca tcacgttcgg tctcgacacc gatctcgacg
     1561 atgccgcgcg tcaggcgctc aaggaaatga tcgactggat cgtcagcatg accggcatcc
     1621 acaaggacga ggcctacgcc atgtgcagct tcgcgggcga tctgcacgtg acgcagacgg
     1681 tgaacaatgt caaaggcgtg catgcaatgc tccagaagag catactgaag aaatagcgcg
     1741 cgcggcgccg ttctcatcaa ggccggcgaa cgcgcgcttc tgatcgatgg cgactcgact
     1801 cgcacagcgc tgaccacggc gttgatatca gatcggcagg cggcaaaaat cgaccttctg
     1861 cacggcgatt ccggacacag gtttcggccg ttgacggtgg acgggttcga ttttctcccg
     1921 gcggcccgat cctcaggtgt cgacgatctg ctgacctgca tcaggtcacg cgatgcgttg
     1981 gcacgcctcg gcgaggtcta cgactatatt ttagtcgatt tgccgccgat gcttgaatcc
     2041 ggcgagacgg ctacgatgat gacggttgca ggtggctgtg tgcttgttac gcactccgat
     2101 tgctcgtcga ccgatgatgt cgcgcgagcc ctcgaattga gtttgctcga gccggaacaa
     2161 attacggccg ccgcgctaat tacgtcttcg cccgtacaat gaccggaaaa atcgctattc
     2221 cgtgcggaaa aacgccgccg catcggcact gaaagccctg cgcgatttag gatcgagata
     2281 gagcatgtgc ccgccacggt atagcttgag ctgcacgcgc ccaggcggat cgaagaccgg
     2341 catgtgatcg agcacgtagc gcgtcatggc gtaaggcgtg atcatgtcgg aatagccatg
     2401 cgcgatcagc aggcggaacg acggaccgaa cgccagcagc acgcgcagat catcttccgc
     2461 gctcgcctgc acgcgaccgc cctgccaatc ccagtggctg gtgacgtcgc cggcgagaag
     2521 cgcataggtc atctcggttt tgaaaccgag ctcgttgcgc gcatacgctg ccatggcacc
     2581 accatagctg cgcgtgattg catcaagaat tggatccgga ccgcgcgacg aacgggactc
     2641 gggatagggg tcgtccacgg caaaagccgc gtcgtatcgg ctcacgactt tgccttcgct
     2701 tgcgcgcagg ttcttcacat aggccctggc agcaaagccg cgcgcctggg tgatagcgtc
     2761 aggcggcaaa cctgtcattt gcgccacgcg gctgtagaac gtccttgctg tatcgtcttt
     2821 cgggggcgct cccgccagag tcgtcaaata gtcggtcatc gcaaactttt ccgccgcggc
     2881 cagcgcctcg gcggtgaacg catgtctgcg ctccagttcg gcggcggcca gcgacggcag
     2941 ctgcagggcg gcgtgcagcg cggagccgtc gtcgccgaaa gtcagccagc cctcgagcaa
     3001 aggcgagagc atgacgatgc cgttgatcgt tatgccttga tcccgttgca gtgctcgcgc
     3061 tgtttttgcc gcgcggaaac cgccgtaact ttcaccgaat agatatttcg gcgagccggt
     3121 gcggttgttc ttggcgacgt aaagtgcaat cgccttggcc atagcatcgg cgtcgctgcg
     3181 tataccccag aaatgcttgc tgtcttcact tttgactgcg cgactccagc cggtgccgat
     3241 cggatcgatc atgacgaggt cggtaaaggc gagccaggta tcggggttgt cgcgcaagac
     3301 cgcgcgggtg gcgtcgtgcg gatccgatcc aaaatcgaga attcgggggc cgacaaggcc
     3361 aaggtggaga aaagcagacg ccgcgccagg accgccattg aacgcaaacg tgagcggacg
     3421 gtttgcagcg ccgttcttcg ccacgtaggc ggtgtagaag actgacgcac tctgctcgcc
     3481 ggattgatca tagaacgcca gcgtgccggc ggtcgccgaa taggcgagct ttccgcgcgg
     3541 cgtgtcgatg gagtggtcgg agatcgcgtc gggcggcagc agccgaagca cacccttggc
     3601 cgccacgtgc cgttcggcac cttgttcgac cgccggctga gcacggtttt tggcggcgtc
     3661 gtcggcgttt gcgggatgaa gtgcgctggc cagaaccagc gccaacgcga agccgcggag
     3721 gatgaagggg gacatcggcg cgtgcaactc ctgtgatgtc ccccgaaacc ccatgtggcg
     3781 gccgcctaac gccggccgcg gaaattcggc cggcgttgct gcggggcagg ggacggcgcg
     3841 tcgcctttgt ggcggaaact gatgcgaccg cgctgcaggt catagggtga catttccacg
     3901 attacgcgat cgccgacgcc ggtgcggatg cggaacttgc gcatcttgcc agctgtgtag
     3961 gccagaacct cgtgatcgtt gtcgagtttc acccggaaat ttccgtccgg gagaacctcg
     4021 gtaaccgtac cgtcaaactc cagcagttct tctttcgcca tccagtgacc tccagttgcc
     4081 tcagttcggg ctgcggccgg cattgcggcg cggctcgccc gggcgctgca taaatctaac
     4141 atttcccaat tcgtttggct cgcccttcgc gtgacc
//
LOCUS       DASSRL010000314         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_515957, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000314 DASSRL010000000
VERSION     DASSRL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515957"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VFL49_06470"
     CDS             complement(<1..170)
                     /locus_tag="VFL49_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7911654.1"
                     /translation="MAYRDFVSLVHKATKRDYLARVTQRDKAEVAELALQWGYDYWDG
                     SRETGYGGYRYDG"
     gene            complement(189..1790)
                     /locus_tag="VFL49_06475"
     CDS             complement(189..1790)
                     /locus_tag="VFL49_06475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET7911655.1"
                     /translation="MRIGIDFDNTIVDYEGAFHAAAMERGLVTADLPKTKNSVRDFLN
                     GAGRKDDFTALQGYIYGTRMDLARVYPGFREFVAAARASRHDVFIVSHKTRFPLLGPK
                     YDMHQAARAFLAGHMLSEDHAVPRQNIFFEETKEQKINRAAVLQVDVFIDDLPEMLVM
                     RGLPERCRRILFAPHGSDARGNFEQCRRWDEITALLFGARENVLPHEMARTLAERAGL
                     GVPKSVESLIGGRNNRVFRVDFKSGEAAVLKSYHHDPRDPRDRLRAEWSFLNYVTARG
                     VQNVPRPLAIDAATHSALYSFVIGERPKKVDGDLIRQAAEFAVAINRAPNEAGSLDPA
                     SEACFSLASHLETVDRRVVRLRELDPNIPHVEAAQKFVDDRLKVMWDRVKSGVIRQAA
                     ERGISRTNSVRAIVSPSDFGFHNSLINMDGEASFLDFEYAGLDDPAKLICDFFCQPEL
                     PAPIRHYAEFTERLVRPLALQDEDLWRARALLDAYRVKWVCIMLNEFSPLGARRRAFA
                     SSDESGAHAAQQLRNAEHYFELISP"
     gene            complement(1795..>2403)
                     /locus_tag="VFL49_06480"
     CDS             complement(1795..>2403)
                     /locus_tag="VFL49_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7911656.1"
                     /translation="CSAPTGMGAILNVLNVKAGNSVVVFGTGGIGLNAIMAAAHVDGD
                     PVIGVDINASRRDLALAFGATHVIDPGAGDTRAQIKTIAPQGIDVAVEATGNPAVMAE
                     ALTMTRMRGGRAVVIGNARHDAVFTVSPSVFNEGKSLLGTWGGDSIPDRDYARYASLL
                     SNSRFAVERLLSAPYRLEDINQALGDFRAGTITRPLIDMSKG"
BASE COUNT          509 a          734 c          687 g          473 t
ORIGIN      
        1 ccgtcgtagc ggtagccgcc gtaaccggtc tcccggctgc cgtcccagta gtcatatccc
       61 cattgcaacg ccaactcggc gacttccgcc ttgtcacgct gtgtaacacg cgcaaggtag
      121 tcccgcttgg tggccttatg aacgaggctg acaaaatcac gataggccat tagcgatctc
      181 cgacaaattc aaggcgagat gagctcgaag tagtgttccg cgttccgaag ctgctgcgcg
      241 gcatgagccc cgctttcgtc gctcgatgca aaagcccgcc ggcgggcgcc taacggggag
      301 aactcgttga gcatgatgca tacccatttg actcgatagg cgtcgagcag cgcccgcgcc
      361 cgccacaagt cttcgtcctg cagggccagt ggcctcacaa gccgctccgt aaactccgca
      421 tagtgccgga tcggtgccgg caactcgggt tgacagaaaa aatcgcaaat aagcttggcg
      481 ggatcgtcaa gcccggcgta ttcgaagtcg agaaaagatg cttcgccatc catgttaatc
      541 agcgaattat gaaatccaaa atccgaaggc gacacgatcg ctcgcaccga attggtccgg
      601 gaaatgcctc gttccgctgc ctgccggatc actcccgact tcacgcgatc ccacatcacc
      661 ttaagacgat catcgacaaa tttttgagcc gcctcaacat gcggaatgtt cgggtcaagc
      721 tcgcgcagcc ggacaactcg acgatcaact gtctccagat ggcttgccag tgagaaacag
      781 gcttcggacg ccggatcgag actcccggct tcattcggag cccggttgat cgccacggca
      841 aattcggcag cctgccgaat gagatcaccg tcgacttttt ttggccgctc ccctatgacg
      901 aagctataaa gcgcgctgtg cgtggccgcg tcgatggcca acggccgcgg cacattttgc
      961 acgccgcgcg cggtaacata gttcaggaag ctccattccg cgcggagccg atcgcgtgga
     1021 tcgcgcgggt cgtgatggta cgatttcagc acggcggcct cgccgctttt gaaatccacg
     1081 cggaagaccc ggttgttgcg gccgccaatc aaggactcaa cgctttttgg gacgccgagg
     1141 ccggcgcgtt cggcgagcgt tctagccatt tcatgcggca ggacattctc gcgagcaccg
     1201 aaaagcaacg cggtaatttc atcccagcgc cggcattgct cgaagttgcc gcgggcatcc
     1261 gagccatggg gagcaaaaag aatgcgccgg caccgctccg gcagtccccg catcaccagc
     1321 atttccggca gatcgtcgat aaagacatcc acctggagca ccgccgcacg gttgattttc
     1381 tgctccttgg tctcttcgaa aaaaatgttc tgtcgtggaa ctgcgtggtc ttccgataac
     1441 atgtggccgg ctagaaatgc gcgcgccgct tgatgcatgt cgtatttcgg cccgagcaaa
     1501 ggaaatcgag tcttgtggct gactataaaa acatcatgcc gagatgcgcg agcggctgcg
     1561 acgaattcgc ggaagccggg gtaaacccgg gcaagatcca tccgtgtgcc gtagatgtat
     1621 ccctgcagcg cggtgaaatc gtccttgcgg ccggcgccgt tgaggaaatc gcgaaccgaa
     1681 tttttggtct ttgggagatc ggctgtgacc agcccacgct ccatggccgc agcatgaaac
     1741 gccccttcgt agtccacgat cgtattgtcg aaatcaatgc cgatgcgcat ggcatcagcc
     1801 ttttgacata tcgatcaatg ggcgggtgat cgtgcccgcg cgaaaatcac ccagcgcctg
     1861 attgatgtct tccaggcggt aaggcgcgga caaaagccgt tccaccgcaa atcggctgtt
     1921 ggaaagcagg ctcgcgtaac gggcgtagtc gcggtccgga atactgtcgc cgccccaggt
     1981 ccccaacagg ctcttgccct cgttaaaaac ggacgggctc acggtgaaga ctgcgtcatg
     2041 acgcgcgttg ccgatgacga ccgcgcggcc tccgcgcatg cgggtcatag ttagagcctc
     2101 ggccatgaca gcgggatttc cggtcgcttc gacggcgaca tcaatgccct gcggcgcaat
     2161 ggtcttgatt tgtgcgcgcg tgtcaccagc ccccggatcg atgacgtggg tcgcaccaaa
     2221 ggcgagcgcc agatcgcgac gcgacgcatt gatgtcgacg ccgatgactg gatctccatc
     2281 gacgtgtgcc gcggccatga ttgcgttcag gcctataccc ccggtgccga agacaaccac
     2341 cgaattacct gccttcacat tcagaacgtt caggatcgcg cccatacccg ttggcgcaga
     2401 gca
//
LOCUS       DASSRL010000315         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1075916, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000315 DASSRL010000000
VERSION     DASSRL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075916"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFL49_06485"
     CDS             complement(<1..849)
                     /locus_tag="VFL49_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7911657.1"
                     /translation="MPDRAEGHERKAFDKSQTAPAGSSIILLLLVALALISAAGGLIL
                     VGRDYVEGYILTLLAILGTIGVFALFALAAGIVKFTGKDQGNPLLKAVVDGAFDGLLV
                     TDQNGRVIYANATYLDLTGATDMRDVRPIERVFIGDPDVSESIYRLLKAAREGRRLQE
                     EVRITGTAGENARWLRMRVRPLGEKRRDARMTVWSIADVTRDRERQENVFQELQYAID
                     YLDHAPAGFFSVDAAGDIVYLNATLASWLDHDLAQVGAGSLKLGDIVAGEGAALLTTF
                     TAAPGEV"
     gene            complement(945..2033)
                     /gene="flhB"
                     /locus_tag="VFL49_06490"
     CDS             complement(945..2033)
                     /gene="flhB"
                     /locus_tag="VFL49_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HET7911658.1"
                     /translation="MAEESGEFDKTEEPTQKRLEEALKRGDVVKSQEVNTWFVIAGAV
                     LVLMAFSGPMSRDLTATMRGIIANSYSISVDGPALPRLFQKIGSEMVAAVAVPFIVLI
                     VAAIAGNIIQHRLVWSYQTVAPKLSKISPLSGLKRIFSKQALANLAKGLVKLAVVGSV
                     MTVLMWPERGRLEALTRSDTAAIIPVSLTLALKMLGAVVAMLALVAAADYLFQYRQWY
                     ERQKMSLRELKEEFKQSEGDPAIKGKMKQVRQIRMRRRMIAAVPKATVVITNPTHYAI
                     ALEYERGMDAPVCLAKGVDALALKIREVAGKHSVPIVENPPLARALHATVEIDQQIPP
                     EHYKAVAEVIGYVMRLRRAAFLRRSERI"
BASE COUNT          426 a          658 c          610 g          407 t
ORIGIN      
        1 tacttcgccg ggggccgcgg tgaatgtcgt cagcagcgcc gccccttctc ccgcaacaat
       61 atctccgagc tttagcgagc ctgcgccgac ctgggcgaga tcgtggtcga gccacgatgc
      121 cagcgtcgca ttcagataga cgatgtcacc tgcggcatcg accgaaaaga agccagccgg
      181 tgcatggtcg agataatcga tcgcgtattg caactcctga aacacgtttt cctggcgctc
      241 gcggtcgcgc gtgacatcgg caatcgacca tactgtcata cgggcgtcgc ggcgtttctc
      301 tccgagaggc cgtacgcgca tgcgcaacca gcgggcgttt tctccggcag ttccggtgat
      361 cctcacctct tcctgcaggc gccggccctc ccgagcggcc ttaagaagcc tgtagatcga
      421 ttccgaaacg tcggggtcgc cgatgaacac gcgctcgatc ggacggacat cgcgcatatc
      481 agtcgcaccc gtcagatcga gataggtcgc gtttgcgtaa atgactcggc cgttttggtc
      541 cgtcaccagg aggccgtcga aggcgccgtc caccaccgcc ttgagcaggg gattgccctg
      601 gtccttgccg gtgaatttca cgatgccagc agccaaggca aacagtgcga aaacgccgat
      661 cgttccgagg atagcgagca gcgtgagaat gtagccttcc acgtagtcgc ggccgacaag
      721 aattagtcca ccggccgcac tgatcaacgc caaagcgacc agaagcaaca ggataattga
      781 actgccggcc ggcgctgtct ggctcttgtc aaaagccttg cgctcgtgcc cttccgcacg
      841 gtccggcatc tgcgccgacg ctgagggatc gtggcttgcc atagtccctg agtgccttaa
      901 ggcgacctgt ccgcgcaagt gccagtccgg ctgattccag gcatttaaat acgctctgag
      961 cgccttagga acgccgcccg gcgcagccgc atcacatagc cgatcacctc agccaccgcc
     1021 ttgtagtgct cgggaggaat ctgctgatca atttccacgg tagcatgcag ggcgcgggca
     1081 agcggcggat tttccacgat cgggaccgaa tgctttccgg cgacctccct gatcttcagg
     1141 gccagcgcat ccacgccttt tgccaggcac accggcgcat ccatgccgcg ctcgtattca
     1201 agcgcgatgg cgtagtgcgt cgggttggtg atgacgaccg tggctttcgg gacggctgca
     1261 atcatgcgcc gacgcatccg gatctgccgg acctgcttca tcttcccctt gatcgccgga
     1321 tcgccttccg actgtttgaa ctcttccttc aactcgcgca gggacatctt ctgccgctcg
     1381 taccactgcc ggtactggaa caggtagtcg gcagcagcga ccaaagcgag catggcgacc
     1441 accgcgccga gcatcttcag ggcgagggtc agcgagaccg ggatgatcgc tgcggtgtcg
     1501 ctgcgagtca gcgcctccag ccgcccccgt tcgggccaca tcaacaccgt cataaccgaa
     1561 ccgacgacgg ccagcttgac gagacccttg gccaggttgg ccagcgcctg cttggagaaa
     1621 atgcgcttga gccccgagag cggagaaatt ttggagagct tgggggcaac agtttggtaa
     1681 gaccagacga gccgatgctg aatgatattg ccggcaatcg cagcgacaat cagaacgatg
     1741 aatggaacgg caacagctgc aaccatctcg ctcccgattt tctgaaacag ccgcggcaag
     1801 gccgggccgt ccacgctgat actgtaggaa tttgcaatga tgccgcgcat ggtggccgtt
     1861 agatctctgc tcatcggacc ggagaacgcc atcaggacaa gtacggcacc ggcaatgacg
     1921 aaccaggtat tcacctcctg gcttttgacg acgtcgccgc gcttgagcgc ctcttcaagc
     1981 cgcttctggg tcggttcttc tgttttgtcg aattcgccgg attcttccgc catgcaagcc
     2041 ccccgtcaga aacgtggagc gagatcggaa agaacgccgt tgagataatc gacaaacgta
     2101 c
//
LOCUS       DASSRL010000316         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1407944, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000316 DASSRL010000000
VERSION     DASSRL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407944"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /gene="tcuA"
                     /locus_tag="VFL49_06495"
     CDS             <1..1226
                     /gene="tcuA"
                     /locus_tag="VFL49_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HET7911659.1"
                     /translation="MGRVTEYRTDPDLCEVLITRSKDTMLWMRKKGIRFIPIYGRQAY
                     KIDGKFKFWGGLTVESWGGGPGLIEAHTAIAKKNGVTVAYNARALSLIADDDGVKGIR
                     VKHDGRTVDIKAKCVVLAAGGFQANSEMRTRYLGPGWELAKIRGTRFNTGDGIRMALE
                     IGAMPTGNWSGCHAVGWDRNAPEFGDLAVGDNFQKHSYPWGIMINANGERFVDEGADF
                     RNYTYAKYGRVILMQPGQFAWQVFDKKIIPMLRDEYRIKQVTKVRADTLEELVKRLDD
                     VDARRALETIKAYNKAVRTDVAFNPNVKDGRRTEGLAVNKSNWANTIDEPPFEAYAVT
                     CGITFTFGGLKIDTSGRVIDTDGQVIAGLHAAGELVGGLFYFNYPGGTGLMAGSVFGR
                     IAGTTAGQRAAQTPA"
     gene            complement(1254..2411)
                     /locus_tag="VFL49_06500"
     CDS             complement(1254..2411)
                     /locus_tag="VFL49_06500"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET7911660.1"
                     /translation="MPHSIAPAGTADRRQQGGGSRPAILDSHQSLIDELEAAISQRNI
                     ASRAEILRRITDLFAAGSGNFGGEQTSLFDDVMGRLVNEIDHSARAAFGERIAGLSNT
                     PPRITRELALDESIDVAGPVLRRSNSLDEDTLITGARTKGQDHLFAISQRSQLSESVT
                     DVLVERGNQKVVISTAANTGARFSEFGYTKLVSRSQTDSELALLVWSRPEIPRDYLLR
                     LFESASEAVQQRFEAADRTRADLVHEMIKRAADQLQTSLRDHSSDFAAARAAIEELHK
                     KGKLTELQLYRFAERGKFDETAIALAILTDLPVGAIERALVHDTGDQVLVLARSIDLS
                     WRTTRAILAVRSGAGGEFPDYHERFNKLRAETARSAIQFYRLRERASKPQK"
     gene            complement(2401..>2636)
                     /locus_tag="VFL49_06505"
     CDS             complement(2401..>2636)
                     /locus_tag="VFL49_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7911661.1"
                     /translation="NLTKRTDCAAIIAATLTLARSLDIETTAEGVETADQYRLLRLAG
                     VTSLQGFLFQAPCPSAEIDFSSAYNIPEIENAA"
BASE COUNT          523 a          811 c          810 g          492 t
ORIGIN      
        1 acatgggccg cgtgacggaa taccggaccg atcccgacct gtgcgaggtc ctcatcacgc
       61 gcagcaagga cacgatgctc tggatgcgga aaaagggcat ccggttcatc ccgatctacg
      121 gccgccaagc ctacaagatc gacggcaagt tcaaattctg gggcggtctc accgtggaat
      181 cgtggggcgg cggcccggga ttgatagaag cacacacggc gatcgccaaa aagaacggcg
      241 tcaccgttgc ttacaatgcg cgcgcactgt cacttatcgc cgacgacgac ggcgtcaagg
      301 gcattcgtgt caagcacgac ggccgtactg tcgacatcaa ggcaaaatgc gtggtgctcg
      361 cagccggagg cttccaggca aacagcgaga tgcgcacccg ctaccttggc cccggctggg
      421 aattggcgaa gattcgcggc acccgcttca acaccggtga cggcatccgt atggcgctcg
      481 aaatcggcgc gatgccgacg ggcaactggt ccgggtgcca cgccgtaggc tgggaccgca
      541 atgcgcccga gttcggcgat cttgcggtcg gcgataattt ccagaagcac tcctacccct
      601 ggggcatcat gatcaatgcc aatggggagc gcttcgtcga cgagggcgcc gacttccgca
      661 actacaccta tgcgaagtac gggcgcgtca ttctcatgca gccgggtcag ttcgcctggc
      721 aggtcttcga caagaaaatc attccgatgc tgcgcgacga gtaccggatc aagcaggtca
      781 ccaaggtccg cgccgatacg ctcgaggaac tcgtgaagcg gctcgacgat gtcgacgcca
      841 ggcgcgcgct ggagacaatc aaggcctaca acaaggcggt gcgcaccgac gtcgccttca
      901 atccgaacgt caaggacggg cggcggaccg aaggccttgc cgtcaacaaa tcgaactggg
      961 ccaacacgat cgatgagccg ccgttcgagg cctatgccgt gacctgcgga atcactttta
     1021 cctttggtgg gctgaagatc gacacctccg gccgcgtgat cgacaccgac ggccaggtta
     1081 tcgccggcct gcacgcggcg ggcgaactgg tgggcgggct gttttatttc aactaccccg
     1141 gaggcaccgg cctgatggcc ggttcggtct tcggcagaat cgccggcacg accgcgggtc
     1201 aacgggccgc ccaaacgccg gcctgacggc gcgcgcgcct gtcggcgcaa aagctatttc
     1261 tgcggtttgg acgctcgttc gcgcaaccgg tagaactgga tcgctgaacg cgccgtttcc
     1321 gctcgtagct tattgaagcg ctcgtgatag tcggggaatt ccccgcccgc cccgctgcgg
     1381 acagcgagaa tggcgcgcgt cgttctccag gaaaggtcga tcgatctcgc gagcacgaga
     1441 acctgatctc ctgtatcgtg caccagcgca cgctcaattg cgccgaccgg caaatcggtg
     1501 aggatggcca aggcgatcgc cgtctcgtcg aattttcccc gctctgcaaa ccggtaaagc
     1561 tgcaactcgg tcaacttccc cttcttgtga agttcctcaa tggcggccct cgctgccgcg
     1621 aagtcggatg agtggtcgcg caggctggtt tgcagctggt ccgcggcgcg cttgatcatt
     1681 tcgtgcacga ggtcggccct ggtccggtcg gccgcctcga atctctgctg tacggcctcg
     1741 gaagcggact caaagaggcg caacagataa tcgcgcggaa tctcgggacg tgaccacacc
     1801 aggagtgcaa gttcgctatc ggtttgcgag cgtgacacca gcttcgtgta gccaaactcc
     1861 gaaaaccgcg cacccgtatt cgccgccgtg ctgatcacta ctttctggtt tccccgctcg
     1921 acaagaacgt cagtgacact ttcgctcagc tggctgcgct gagagatggc aaaaagatga
     1981 tcctgacctt ttgtccttgc accggtaatc aaggtgtctt catcgagaga gttggaccgc
     2041 ctcagcaccg ggccggcgac gtcgatagac tcatcgagtg ccagttcgcg ggttatcctg
     2101 ggcggcgtat tcgacaagcc ggcgatgcgc tcgccgaacg cggcgcgcgc ggaatggtcg
     2161 atctcattga ccaaccggcc cataacgtcg tcgaacaggg atgtctgttc gccgccaaaa
     2221 ttgcctgacc cggcggcaaa caaatcggtg atgcgccgca gaatctcggc gcgagacgca
     2281 atatttctct gcgaaattgc ggcctcaagc tcgtcaatca gcgattgatg cgaatccaga
     2341 atggcggggc gactcccgcc gccttgctgg cggcgatccg cagtacccgc gggtgcgatg
     2401 ctatgcggca ttttcgattt cagggatgtt ataggcgctg ctgaaatcga tttccgcgga
     2461 agggcacggc gcctggaaca ggaagccctg cagcgatgtc acgccggcga ggcgcagcag
     2521 ccggtactga tccgccgttt ccacgccttc cgcagtcgtt tcgatgtcga ggctgcgcgc
     2581 gagcgtcagc gtcgccgcaa ttatggcggc gcagtccgtg cgctttgtca gatttt
//
LOCUS       DASSRL010000317         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1565908, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000317 DASSRL010000000
VERSION     DASSRL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565908"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1458)
                     /locus_tag="VFL49_06510"
     CDS             complement(46..1458)
                     /locus_tag="VFL49_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7911662.1"
                     /translation="MSRTLIKNGFVVTVDGSRAVHPGGFVATDGARITGVGPSSKTPD
                     AKGFDEVIDLSGCIVLPGLINGHQHHWYTLFKGLADGYLLEDWVSGFLLPLTRHLSEE
                     EMRCSSYVAAMEMLATGTTCSLNHSVTTTTPEIVRATIEPQAELGIRQVFAKELRCRT
                     TGNPHHPFALDESLAAFEEEFKRWDGKADGLVRMAMVIESNAHWVAAGMTTEELIGRG
                     YELARRLKTKISTHIAGGTFSIEKGFLRYLRETGRTDVRYLMQLGLLDPHWVLIHGIH
                     VTELDIEHMARVGCSFVYTPTSESIRGGGIGPFANAWRAGVNVALGTDGPMVDYSVDM
                     VEQIKACMLMQHVRHLDPTRMPVERSIEMATINAAKALGLDHEIGSLEAGKRADIAVF
                     DMARPYIGVLHRPISTFICAGKGSDARLVMVDGQIVYRDGVFPRFKDGDRVIRDAEKI
                     GKKTLDAAGLSGRLEPAWRQ"
     gene            complement(1632..2369)
                     /locus_tag="VFL49_06515"
     CDS             complement(1632..2369)
                     /locus_tag="VFL49_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HET7911663.1"
                     /translation="MSIIIALMVLSLAGLLFLPPITQDQNYHDFADRRTILGVPHFWN
                     VVSNLPFIVVGATGLRRFHRHPATFVLFLGILMTGFGSVYYHLDPNDRTLFWDRLPMA
                     VSFMAILANAVEDRIDERAGRILLWPLIATGVLSLFLWRWTGDLRLYGWVQFFPCVAL
                     VLLFVLLPAKYSGTSYWFIAGALYALAKLFEFYDREIYSVGHILSGHTLKHIAAAAAC
                     LAILRYFQNRRLLPAGQGSSPSRAAPA"
BASE COUNT          584 a          765 c          744 g          505 t
ORIGIN      
        1 gttctcgacc gacacgtcca tagcgcgggg tacccgaatt gttcgctatt gccgccatgc
       61 cggctccagc cgaccgctta atcctgccgc atccagcgtt ttcttgccga tcttttcggc
      121 gtccctgatg acacggtcgc cgtccttgaa gcgcgggaac acgccgtcgc gatagacgat
      181 ctggccatca accatgacca gccgcgcatc actgcccttg ccggcgcaga tgaaagtcga
      241 aatcggccgg tggagcacgc cgatataggg cctggccatg tcgaaaaccg cgatatccgc
      301 gcgctttccg gcctcaagcg agccgatctc atgatcgagc ccgagcgcct ttgcggcatt
      361 gattgtggcc atttcaatcg agcgctccac gggcatgcgc gtcggatcga gatggcggac
      421 gtgctgcata agcatgcacg ccttgatctg ctcgaccatg tccacgctgt agtcgaccat
      481 cggcccatcg gtgccgagcg cgacattcac gccggctcgc catgcgttgg cgaagggtcc
      541 gatgccgccg ccgcgaatgg attcgctggt gggcgtatag acgaagctgc agccgacgcg
      601 cgccatgtgc tcgatatcga gctccgtcac atggatgccg tgaatcagga cccaatgcgg
      661 atccagcaga ccaagctgca tcaggtagcg gacatcggtg cgcccggttt cgcgaaggta
      721 gcggagaaag cccttctcga tcgaaaacgt gccgccggcg atgtgggtcg agatcttcgt
      781 tttgagtctt cgcgcaagct cgtaaccgcg gccgatgagc tcctcggtcg tcatgccggc
      841 ggccacccaa tgcgcatttg actcaatgac catcgccatc cgtacgagac catcggcctt
      901 gccatcccag cgcttgaatt cctcctcaaa agcggcgaga gattcatcaa gcgcaaatgg
      961 atgatgcgga ttgcctgtgg tgcggcagcg gagctctttc gcgaaaacct gacggattcc
     1021 gagctccgcc tgcggctcga tggtcgcgcg tacgatttcc ggcgtagtcg tggtcaccga
     1081 gtgattgagt gagcatgtgg tgccggtcgc cagcatttcc atcgcggcga cgtagctcga
     1141 gcaccgcatc tcttcttcgg acaggtggcg cgtgagcggc agcaaaaagc cgctcaccca
     1201 atcttcgaga aggtaaccgt ccgccaggcc cttgaagagc gtgtaccagt gatgctggtg
     1261 gccattgata agccccggca gaacgatgca gccggatagg tcgatgacct cgtcgaaacc
     1321 ttttgcatcc ggcgttttcg aagacggccc gacgccggtg atcctggcac cgtccgtcgc
     1381 aacaaagccg cccggatgaa ccgcgcggct tccatcaact gtgacaacga atccgttttt
     1441 tatcagcgta cgcgacattt acccgtctcc gaggacagcc tttatccaaa atatttccgt
     1501 gtcaactcaa tgcgagttgc atggcgggag cagcggtctt gaccgcgcga ctggatcaaa
     1561 gaaaacattc cagcttgatc attgcgaatc gaaattgact cttcgattac gctgctgcag
     1621 ttgcaggacg atcaggccgg cgctgcgcgc gacggggagc tgccctgccc cgccggaagc
     1681 aagcgccggt tctgaaaata tctcagaatt gcaaggcagg cagcggccgc ggcaatgtgc
     1741 ttgagcgtat gcccgctcaa aatatgaccg acggaataga tttccctgtc atagaactcg
     1801 aacaattttg caagcgcgta cagggcgccc gcgatgaacc aataagaagt tccgctgtat
     1861 ttcgccggca acaagacaaa aagaagaacc aaggcgacac agggaaagaa ctgcacccat
     1921 ccatacaggc gaaggtcgcc ggtccagcgc cacagaaaca ggctcaggac accggtggca
     1981 atcaaaggcc acaacaatat tcttccggcc cgctcatcaa tccggtcctc gaccgcattc
     2041 gcaagaatgg ccatgaagct gaccgccatc ggcaatcggt cccaaaacag cgtccggtca
     2101 ttcggatcga gatggtaata gaccgagccg aacccagtca taagtattcc aaggaaaagt
     2161 acgaaggttg cgggatgccg atgaaatcgg cgcagccctg ttgctccgac aacaataaaa
     2221 ggcaaattcg atactacgtt ccagaaatgc ggcacgccga gtatcgtgcg ccgatcggcg
     2281 aagtcgtgat aattctgatc ctgggttatc ggcggcagga acagaagacc cgcgagcgac
     2341 aagaccatca aagcgataat gatgctcacg gggcggctgt tttttgaact gacgaacatc
     2401 tgctttgggc accgttcaat cggatcctgc ttaacgtttg atcgtgggca gcgaggcgcg
     2461 aatccggtcg gaaaagtcgg gggcattgtc ggccgcctgc gcgacaccgt tgatgagaga
     2521 gcgccggcgc aattcgtcaa agggcagcgt cgtatctatt cgacccgagt agtaaaggta
     2581 gctgtcgatc agcccgtt
//
LOCUS       DASSRL010000318         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1163566, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000318 DASSRL010000000
VERSION     DASSRL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163566"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFL49_06520"
     CDS             complement(<1..260)
                     /locus_tag="VFL49_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911664.1"
                     /translation="MTGLWTAANSGQKRLTETADILVVAIAVSLPWSTSATSVLIALW
                     FLALLPTLRWAEIRHEAETLAGGLPVVLVALGLAGMLWSDVT"
     gene            complement(264..1088)
                     /locus_tag="VFL49_06525"
     CDS             complement(264..1088)
                     /locus_tag="VFL49_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7911665.1"
                     /translation="MAELISVIVTTFNREDALDAVLRSLSIQGDGDFEVIVADDGSDA
                     ATARLIDAWKPKFAHRLEHVWHADQGFRAAEIRNRAILAARGSYCIFLDGDCIVRPDF
                     VATHRRLATHGWFVTGNRVLLSPALTTSVLRENRTPETWGAGRWLGERSRGGVNRLSA
                     LLTLPLGILRRLRKNAWQGARSCNLAIWRSDLDRVDGFDADYRGWGKEDSDIIARLLH
                     AGIRRKDGIFATGVIHLWHPEAERSQLARNENRLADVLAGDRVRAVRGLSALQGAS"
     gene            1205..1999
                     /locus_tag="VFL49_06530"
     CDS             1205..1999
                     /locus_tag="VFL49_06530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7911666.1"
                     /translation="MQKISAYILTYNEAEKIEAAVSSVLWADEVVIVDSFSADRTAQI
                     ARSLGARVVDLPFEGFGELRNRAIEACEFEWIFSLDADERCTEAVRDEILALLAGSPP
                     HDIYMVPRRSYMMGRWIKGSGWYPNFRQPQLFRKGSIRYTLEPVHEGYEVLGKRSVGS
                     LKNAVWQFPFRNLEEVINKMNRYSTLGVPKLAHKRVSMGSALGHGIWSFLKHYIFKLG
                     FIDGWAGFVIALGNFEGTFYRYAKRYEQMQAWKPPASGAIRREASL"
BASE COUNT          426 a          643 c          631 g          368 t
ORIGIN      
        1 aatgtgacat cggaccagag cattccggcg agaccaagcg caacgagaac caccggcaac
       61 ccgccggcca acgtttcagc ttcgtgccgg atctccgccc agcgaagcgt cggaagcagt
      121 gcaagaaacc atagagcaat gaggacagag gtagcggacg tggaccacgg gagggaaaca
      181 gcaatcgcga caacaagaat gtccgccgtc tccgtcagcc gcttctgacc cgaatttgca
      241 gcggtccaga gtcctgtcat tgctcaactc gcgccctgca gcgccgacag cccgcgaacc
      301 gcccgcacgc ggtcgcctgc caggacatcg gcaagtctgt tctcgttccg cgcgagttgc
      361 gaccgctcgg cctccgggtg ccaaagatga atgacgccgg tggcaaatat gccgtccttg
      421 cggcgtatgc cggcgtgaag cagccgggcg ataatatcgg agtcctcttt tccccagccg
      481 cgatagtccg cgtcaaaacc gtcgacacga tcgagatcgg atcgccagat cgcgagattg
      541 cacgagcgcg caccctgcca ggcatttttt cggagtcgcc gaagaatgcc gagcggcagg
      601 gtcagaagtg ccgacaaacg gttgacgccg ccgcgcgagc gctcgccaag ccagcggccg
      661 gcgccccagg tttccggagt cctgttctcg cgcagcacgc tcgtcgtcag cgccggggag
      721 agcaagacac ggttgccggt gacgaaccag ccgtgcgtcg ccaggcgacg gtgtgtcgcg
      781 acgaaatcgg ggcgcacgat gcaatctccg tccagaaaaa tgcagtagct gccgcgcgct
      841 gcgagaattg cgcggttgcg gatctccgcc gcgcggaagc cctgatcggc gtgccagaca
      901 tgttcgaggc ggtgcgcaaa tttcggcttc caggcgtcga tcaacctcgc ggtcgccgcg
      961 tccgagccgt cgtcggccac gatgacctcg aaatcgccgt cgccctgtat ggaaagcgac
     1021 cgcaacaccg catcgagcgc atcttcgcgg ttgaatgtcg tgacgatgac ggaaatcaac
     1081 tcggccacag gcgccctctt cttccggctt tagccgaagt cgcacatgcc gacaaacccc
     1141 tagggtccgc gcgcgcccca atgttacctt gccgtctatc cggagccttc gcaggcaagc
     1201 gattatgcag aaaatatcag cctatatcct cacttataat gaggcggaga agatcgaggc
     1261 ggccgtatcg agcgtattgt gggccgacga ggtcgtcatc gtcgactcgt tcagcgccga
     1321 tcgcaccgcc caaatcgcac gctcgctcgg tgcccgcgtc gtggacctgc cgttcgaggg
     1381 cttcggagag ctgcgcaacc gcgcgatcga agcctgcgag ttcgagtgga ttttcagtct
     1441 tgatgccgac gagcggtgta ccgaagccgt gcgcgacgag attctggcat tgcttgcagg
     1501 ttcgccgccg cacgacatct acatggtgcc ccggcgcagc tacatgatgg gccgctggat
     1561 caagggatcc gggtggtacc cgaatttccg tcagccgcag ctctttcgca aaggcagcat
     1621 ccgctacacg ctcgagccgg ttcacgaagg ctacgaagtg ctcggcaaac ggtcggttgg
     1681 cagcctgaag aatgcggtct ggcaattccc gttccgtaat ctcgaggaag tcatcaacaa
     1741 gatgaaccgg tactcgacgc tcggcgtgcc caagctcgcg cataaacggg tctcgatggg
     1801 aagcgcactt gggcacggca tctggtcgtt cctcaagcac tatatattca agctcggatt
     1861 catcgacggc tgggccggat tcgtgatcgc gctgggaaat ttcgaaggca cgttttaccg
     1921 ctacgccaag cgttatgagc aaatgcaggc ctggaagcct ccggcaagcg gggcgatccg
     1981 tcgcgaggct agtctctgac aggcttgcgc atccgccgcg acagattgcg cagcatccgg
     2041 aatggcaatg cgaggtaata tttgcgca
//
LOCUS       DASSRL010000319         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_708266, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000319 DASSRL010000000
VERSION     DASSRL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708266"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFL49_06535"
     CDS             <1..651
                     /locus_tag="VFL49_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7911667.1"
                     /translation="PNGAGKTTLVNLLTGLLTPSEGEVLFMGTDIAGVGPVALADHGL
                     VRAFQLIEIFPQLSVAETIAAAVVSRQKKRWRLFARLSADADIAARVREVADIFGLTH
                     RLDTVAATLSQGEKKLLDVASAFALEPQVILLDEPTSGVSTAEKHTIMQTLIAAAKRA
                     GVKGIVLVEHDMDLVAVYSSRIIALSEGQVLADLPPGEFFADPHLIETVIGKRRGH"
     gene            652..1374
                     /locus_tag="VFL49_06540"
     CDS             652..1374
                     /locus_tag="VFL49_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7911668.1"
                     /translation="MLAVRDLAVDIQGSRVLRGVSFQVGQGELVCLVGRNGAGKTTTF
                     RSIMGFRKPLAGSVEFDGRSLLGLRPYEIARLGLGFAPEESEVFGELTVAENIAMPTW
                     TRSEGRPADERIAEAYKVFPRLERYRARGGQALSGGERKMVSIARALALDPRLLLLDE
                     PTEGLSPAIVPSVVEGIAKIRTFGRAVFIAESNIHHVPEFTDRLYVIERGEIIFAGRP
                     AEARRNPAVARVIEGTAQRRPD"
     gene            1507..2226
                     /locus_tag="VFL49_06545"
     CDS             1507..2226
                     /locus_tag="VFL49_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET7911669.1"
                     /translation="MGWYDGYFHIAIIYAMGAAAFYIYVAHIHDVTWLEWLTVPLTFL
                     FTNLFEWAVHKFVMHRPINIKGVRAIYERHTLNHHQFFTDEEMRFRDHKDWRVTVFPP
                     YALVVFILMSIPGAVILGLLFSPNVGWLFMSTTTGMYLIYEFMHFCCHVDENWFVRNC
                     PFVNTLRRHHTAHHNARLMMETNMNLTFPIADWLFGTCDLDRGLIGTLLNGYDTRHLK
                     KNLRGRPRQPDEAAAQPVSSY"
     gene            2240..2434
                     /locus_tag="VFL49_06550"
     CDS             2240..2434
                     /locus_tag="VFL49_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911670.1"
                     /translation="MPNDVKAVVSAIVLIAAAVLAYWSDSPIRPGYGVYTLATAVFMV
                     VAMWIFPEAGVKKGDLRKSN"
     gene            complement(2501..>2733)
                     /locus_tag="VFL49_06555"
     CDS             complement(2501..>2733)
                     /locus_tag="VFL49_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911671.1"
                     /translation="RLHESFRAICSTLTPPLASAVWGGLTAVTTTSAERIRLLYTGNG
                     QTYSLYILYYFIVLYLAGGGIHHLWPSGIAAD"
BASE COUNT          509 a          824 c          849 g          551 t
ORIGIN      
        1 ccaaacggcg ccggcaagac tacgctggtc aatctgctca ccggcctgct gacgccgagc
       61 gaaggcgaag ttctgttcat gggtacggat atcgccggcg tggggcccgt tgcgcttgcc
      121 gaccacggcc tcgtgcgggc cttccagctt atcgagatct ttccgcaatt gagcgtggcg
      181 gagacgatcg ccgcggcagt ggtgtcaagg cagaagaagc gctggcggct ttttgcccgg
      241 ctgtcggcgg atgcggatat cgcggcgcgt gtgcgggaag tcgctgacat cttcgggctg
      301 acgcaccggc tcgacacggt ggctgctacg ctgtcgcaag gcgagaagaa attgctcgac
      361 gtcgcctccg cctttgcgct cgagcctcag gtcattttgc tggacgagcc gacttcaggc
      421 gtttccaccg ccgaaaagca cacgatcatg cagacgttga tcgcggcggc gaagcgggcc
      481 ggcgtcaagg gtatcgttct ggtcgagcat gacatggatc tggtcgccgt ctactcaagt
      541 cgcatcatcg cgctgtcgga gggacaagta ctcgctgact tgccgcccgg cgagtttttt
      601 gccgacccgc acctcatcga aaccgtgatc ggcaagcggc ggggtcactg aatgcttgca
      661 gtgcgcgatc tcgccgtgga tatccagggc agccgggtgc tgcgaggcgt gtcctttcag
      721 gtcgggcagg gcgagctcgt ctgtctcgtg ggacgcaacg gcgccggcaa gaccacgacc
      781 tttcgcagta tcatgggctt tcgcaaaccg ctcgccggct cggtcgagtt tgacggcagg
      841 tcgctgctcg gcttgcgccc ttacgagatc gcgcggcttg gtcttggttt tgctccggaa
      901 gaaagcgagg tcttcggcga actgaccgtc gccgagaata tcgcgatgcc gacctggacc
      961 cgttcggaag gccgccccgc ggacgaacgt atcgcagaag cctacaaagt cttcccgcgg
     1021 ctcgagcgtt atcgggcgcg tggcgggcag gcgctctccg gcggtgagcg caaaatggtc
     1081 tcgatcgcgc gggcgctggc gctcgacccg cggttactgc tgctcgacga gccgaccgaa
     1141 ggactctccc cggcgatcgt gccgtcggtc gtcgagggca tcgccaaaat ccgcacgttc
     1201 gggcgcgcgg tcttcatcgc cgaatcgaat atccaccacg tccccgagtt caccgatcgc
     1261 ctctatgtca ttgaacgcgg cgagattatt ttcgccggca ggccggcaga agcgcgaagg
     1321 aacccggcgg tcgcccgcgt catcgaggga acggcgcagc gccggcctga ttgaaccggg
     1381 gcggccgccg tgatagatgt gtgatatatc aggtgcggcc cggggcggcc gccccggcag
     1441 gcgaggagaa gacacgtgat cagtcagcgg cagatcgatt ttcgccagga ataccgctcc
     1501 cgcatcatgg gttggtacga cggctatttt cacatcgcga tcatctacgc catgggcgcg
     1561 gcggccttct atatctacgt cgcgcatatt catgacgtga cctggctcga atggctgacg
     1621 gtgccgttga ccttcctgtt caccaatctg ttcgaatggg ccgtgcataa attcgtcatg
     1681 caccggccga tcaacatcaa aggcgtccgc gccatctatg agcggcacac gctcaaccat
     1741 catcagttct tcaccgacga agagatgcgc tttcgcgatc acaaggactg gcgcgtcacc
     1801 gtgtttccgc cctatgcgtt ggtggtgttt atcctgatgt cgatcccggg cgcggtcatc
     1861 cttggattgc tgttcagccc caatgtcggc tggctgttca tgtcgacgac cacgggcatg
     1921 tatctcatct atgagttcat gcatttctgc tgtcatgtcg acgagaactg gttcgtgcgc
     1981 aactgcccct tcgtcaacac gcttcggcgc catcacacgg cccatcacaa tgcgcgactg
     2041 atgatggaaa ccaacatgaa cctgaccttc ccgatcgcgg actggctgtt cggcacgtgc
     2101 gatcttgatc gcggcctgat cgggactttg ctcaacggtt acgacacgcg gcacctgaag
     2161 aaaaacctgc gcggccggcc acgccagccc gacgaggcgg cagcgcagcc tgtgagctcg
     2221 tactagaccg gagaccgcca tgccgaacga tgtcaaagcc gtcgtttccg cgatcgttct
     2281 gatagcggcg gcagtgcttg cctattggag cgactcgccg atccggcccg gctatggagt
     2341 ttacacgttg gcgacggctg ttttcatggt tgtggccatg tggattttcc cggaggccgg
     2401 tgtgaagaaa ggcgacctga gaaaatcaaa ctaggcatta acaggcagct cggacgacca
     2461 gtcgtcattg cagaacgcgt cgcgaactcc cgcgcggcaa tcagtctgcg gctatccccg
     2521 acggccataa atgatggatg ccgccgcctg ccaaatacaa gacgataaaa tagtacaaaa
     2581 tatagaggct gtaggtctgg ccattgcccg tatagagcag ccggattctc tcggccgagg
     2641 tcgttgtgac tgccgtcagt ccgccccaga ccgcgctggc caatggcggg gtcaatgtcg
     2701 agcagatggc tctgaaactc tcatggagtc tgg
//
LOCUS       DASSRL010000320         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_918596, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000320 DASSRL010000000
VERSION     DASSRL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918596"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1191)
                     /locus_tag="VFL49_06560"
     CDS             complement(139..1191)
                     /locus_tag="VFL49_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911672.1"
                     /translation="MVGVSLHHVYKRFGELAVVDDVSLEVADQEFLVLLGPSGCGKTT
                     TMRIIAGLETASSGDVYIGGRRVNDLLPRHRDVAMVFQSYALYPHMTVFKNLEYPLKI
                     RKVAAEERRKQVVAAARRVNIEELLERLPKQLSGGQRQRVALGRALIRRPNLFLMDEP
                     LSNLDAKLRVQMRSELKHLQHALKTTTIYVTHDQIEAMTLATRVAVMNKGVMQQLATP
                     KEIYDNPANLFVAGFIGSPSMNFLHGDLSGGVFECKGARVTGLAVRDAGQVTLGVRPE
                     DLTVCGIEDGQIGGEVFSLELTGDSSLLTVQLDNTLVMARVDKDFATEIGAKAWLKAN
                     PNRCFLFDARTEARIA"
     gene            complement(1226..2095)
                     /locus_tag="VFL49_06565"
     CDS             complement(1226..2095)
                     /locus_tag="VFL49_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7911673.1"
                     /translation="MQQSAFTEARLRQFWIKAKGWIAVAVIAIALAQAVFPIIWLGLN
                     SLKNRLEMFARPPIWFTMTVTNENYVATFIDRPFLTYMWNSLVVAACSTLLSMLIGVP
                     AGYALTRFDFGKAKRHIAFWILSTRMTPPIVTVIPIYLLFSYLDLLDTKTSLVIAYTT
                     FNLPFVVWMMRSYLADIPRDLEESAMVDGDTHLGAFWRVVLPLAKPGLAATAIFCLIQ
                     SWNELLFALILTETTNANTLPIGIAGRVTQYKTEWGEIAAAGFAGCLPIIIFAFIVQK
                     HLVRGLSFGAVKG"
     gene            complement(2113..>2482)
                     /locus_tag="VFL49_06570"
     CDS             complement(2113..>2482)
                     /locus_tag="VFL49_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7911674.1"
                     /translation="PFVFLMCLAGLASLSQEPFEAAELDGASAFQKFRDLTLPFLKPV
                     IVVTLLLRIMDAFRLFDQVYVLTLGGPAGATETLSFYLYKVTFRFFDIGYAAAISLFM
                     MFVALQLAGWMLKRTSLHEA"
BASE COUNT          495 a          786 c          776 g          425 t
ORIGIN      
        1 gcaccgtctc gcctgccggc gaaagtgcgg caccgccctg gatggtgatc ggtggattat
       61 caggcagcgg ccaagcctct ccagccagcg cgccgccgcc gcagaggcaa aaggcgactg
      121 ccagcaaccc gcgcaccgtc acgcaatccg cgcctctgtg cgcgcatcga acaggaagca
      181 gcggttcgga ttggccttga gccacgcctt ggcaccgatc tcggtcgcga aatccttgtc
      241 cacgcgcgcc atcaccagcg tgttgtcgag ttgaactgtc agcagcgagg agtcccctgt
      301 cagctccagc gagaacacct cgccgccgat ctggccgtcc tcgatgccac acactgtcag
      361 atcctccggc cgcacgccca gcgtcacctg tccggcgtcg cgcacggcga ggccggtgac
      421 gcgcgcaccc ttgcattcga acacgccgcc acttaggtcg ccgtgcagaa agttcatgct
      481 gggcgagccg atgaagcccg ccacgaacag gttggctggg ttatcgtaga tctccttcgg
      541 ggtcgccagt tgctgcatga cgcccttgtt catgactgcg acgcgggtgg ccagggtcat
      601 cgcttcgatc tgatcgtgag tgacatagat ggtcgtggtc ttcagtgcgt gctgcaggtg
      661 cttgagctcg ctgcgcatct gcacccgcag cttggcatcg aggttcgaga gcggctcgtc
      721 catcaggaac aggttgggcc ggcggatcag cgcgcggccg agggccacgc gctgccgctg
      781 gccgcccgac agttgcttgg gcaggcgctc gagcagctcc tctatgttca cacggcgcgc
      841 ggcggcgacc acctgcttgc gccgctcctc cgccgcgacc ttgcgaatct tcaggggata
      901 ctccagattc ttgaacaccg tcatgtgcgg gtagagcgca taggattgga acaccatcgc
      961 cacgtcgcgg tgacgcggca acagatcgtt cacgcggcgc ccgccgatat agacgtcgcc
     1021 cgatgacgcc gtctccaggc ccgcgatgat gcgcatggtc gtggtcttgc cgcagcccga
     1081 ggggccgagc agcaccagga attcctgatc cgccacctcg aggctgacat cgtcgacgac
     1141 tgcgagctcg ccgaacctct tgtagacgtg gtggaggctc acaccgacca tgtcccctcc
     1201 tccggatttg ccgtcagtat catgatcagc ccttcaccgc accgaacgac aagccgcgca
     1261 ccagatgctt ctggacgatg aaggcgaaga tgatgatcgg caggcagccg gcaaagccgg
     1321 ccgccgcgat ctcgccccac tcggtcttgt actgcgtgac acggcccgca atgccgatgg
     1381 gcagcgtgtt ggcattggtc gtctcggtca ggatgagggc aaacagcagc tcgttccacg
     1441 actggatgag gcagaagatg gcggtcgcgg cgaggccggg tttcgcgagc gggaggacga
     1501 cccgccaaaa cgcccccaag tgggtatcgc cgtcgaccat ggccgattcc tcgagatccc
     1561 tggggatatc ggccaggtag ctcctcatca tccacaccac gaacggcagg ttgaaggtgg
     1621 tgtaggcaat caccagcgag gtcttggtgt cgagcaggtc gaggtaggag aacagcagat
     1681 agatcggaat gacggtcaca atcggcggcg tcatgcgggt cgacaggatc cagaaagcga
     1741 tgtgccgctt ggccttgccg aaatcgaacc gggtcagcgc atatccggcc ggcacgccga
     1801 tcagcatgga caaaagggtc gaacaggctg ccaccaccag gctgttccac atgtaggtca
     1861 ggaacggccg atcgatgaac gtcgccacat agttctcgtt ggtcaccgtc atggtgaacc
     1921 agatcggcgg ccgcgcgaac atctccagcc ggttcttgag cgagttcagg cccagccaga
     1981 tgatcggaaa gactgcctgg gccagcgcga tcgcgatcac tgcaacggcg atccaaccct
     2041 tggccttgat ccagaactgg cggagcctgg cttcagtaaa ggcggattgc tgcaacgtgg
     2101 tctccgccat cgtcaggcct catgcaggga tgtgcgcttc agcatccagc cggcgagctg
     2161 cagtgcgaca aacatcatga agagcgaaat agcggcggcg tacccgatat cgaagaaccg
     2221 gaatgtcacc ttgtagagat agaagctcaa ggtttccgtt gcgccggcgg gtcctcccaa
     2281 cgtcagaacg tagacctggt cgaataggcg gaacgcatcc atgatccgaa gcagcagcgt
     2341 caccacgatc accggcttca ggaatggcaa tgtgagatcg cggaacttct ggaaggcgga
     2401 ggcgccgtcc agctccgccg cctcaaacgg ctcctgcgac aggctggcaa gccccgccag
     2461 gcacatcagg aagacgaagg gc
//
LOCUS       DASSRL010000321         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_376145, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000321 DASSRL010000000
VERSION     DASSRL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376145"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFL49_06575"
     CDS             <1..195
                     /locus_tag="VFL49_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HET7911675.1"
                     /translation="SLFGGALTLAILQFRKLPLPGVLAHQAWIMRLHDSAGGVPYGIA
                     LAAAALAVYPKTGWMPGYGL"
     gene            360..1130
                     /gene="cpaB"
                     /locus_tag="VFL49_06580"
     CDS             360..1130
                     /gene="cpaB"
                     /locus_tag="VFL49_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET7911676.1"
                     /translation="MKAARVVVLTVALAAGGVAALLAGRSDRAPAPKAEAPKSDTVDI
                     LVAKSDIPRGQTLSPADVSWQAWPATTAAGNFIRKSDRPKALENLSGMIVRAPFVAGE
                     PIREAKLVNAKGSGFMAAILPTGMRAVSTQISPETGAGGFILPNDRVDVIMTPRNRDD
                     KGGERTSETILRNIRVLAIDQNVEEKNGQKVVVGKTATLELTPGQAETLALSHQIGIL
                     SLSLRSIADGTLEEKSDDSARQNVNVVRFGVSTTTTPR"
     gene            1152..>2584
                     /locus_tag="VFL49_06585"
     CDS             1152..>2584
                     /locus_tag="VFL49_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HET7911677.1"
                     /translation="MAIKTLTHVASSGALIAFMALTPILPAPAADGPQKAAVKVAASE
                     GTSRSIPLGIGKSVAIDLPTDIKDVLVADPKIANAVVRSSRRVYMIGVAVGQTNIFFF
                     DANGKQIAGFDIAVTRDLNGVRAALKQSMPDSDIHIEGVGTDGIMLSGTAASAAESQH
                     AFDIAARLVGGETKVVNGIAVRGRDQVMLKVTVAEVRRDVIKQLGIDLSGSLNYGTAV
                     VNFNTSNPFSATGQPISDTFFRGAFPSVTATLRAMERAGVIRTLAEPNLTAISGETAT
                     FVAGGEFPIPNGLSCDTTRSPPICQPQITFKKFGVSLVFTPVVLSEGRISLKVMTEVS
                     DLSTENAMTLQVTGSANSLTVPSIQTRRADTVVEIPSGGSLAMAGMIQEQTKQSISGL
                     PALQQVPVLGALFRSRDYINHQSELMVIVTPYVVRAVAQKQLSRPDDGFADPSDPQTV
                     LLGNLNRIYGVAGKSDTLRGNNYHGKYG"
BASE COUNT          530 a          784 c          769 g          501 t
ORIGIN      
        1 tcgctttttg gtggcgcact cacgctggcc atactgcagt tccgcaaact gccgttgccg
       61 ggcgtccttg ctcaccaagc ctggatcatg cgcctgcatg acagtgcggg tggcgttcct
      121 tacggcatcg ctctcgcggc tgcagcgctc gcggtttatc ctaaaaccgg ttggatgcct
      181 ggctacggcc tctaagcgct cgccgtcgtt agcaaggcaa tacaatatta actcgattta
      241 gataccgctc cttaaccata ccttgacctt tagctgttga aatccggtgc agaagccgca
      301 ccggcggcga gtggtttacg cggccagatt ggccgtcagg tcacggtgag agtgagcgta
      361 tgaaagctgc acgtgtcgta gtactgaccg ttgcgttggc cgcaggtggc gttgccgcat
      421 tgctggccgg ccgatccgac agggcaccgg ctcccaaggc cgaggcaccc aagtccgaca
      481 ccgtcgacat tcttgtcgcc aaatcggaca ttccgagagg gcaaacgcta tctccggccg
      541 atgtgagctg gcaggcatgg cccgcgacca cagccgcagg aaatttcatc cgaaaaagcg
      601 atcgtccgaa agcgcttgaa aatctttcag gcatgatcgt gcgtgcgccg tttgtcgcgg
      661 gcgagccgat tcgcgaggca aaactcgtga atgccaaggg ctccggcttt atggctgcaa
      721 ttcttccaac tggcatgcgc gcggtgtcga cgcagatttc cccggagaca ggggcgggtg
      781 gtttcatcct gccgaatgac cgcgtcgacg tcatcatgac cccgcgcaat cgcgacgaca
      841 aaggcggcga gcggaccagc gagacaattc tgcgcaacat ccgtgtgctg gcgattgacc
      901 aaaacgtgga agagaagaac ggccagaagg ttgtcgtcgg caagactgcc acgctcgaac
      961 tcacgccagg gcaagcagaa actttggcgc tatcgcatca gatcggcatt ttgtcgctgt
     1021 cgctgcgaag cattgccgac gggacgctcg aagagaagtc ggatgattcg gcccgtcaaa
     1081 acgtcaatgt tgtccgtttt ggcgtgagca ccacgaccac accaaggtga acgattgggg
     1141 gataagggga catggcgatc aagacactaa cccacgttgc ctcatcaggc gcgctgatcg
     1201 ccttcatggc gctcaccccg atcttgcctg cccccgcagc ggacggcccg caaaaggccg
     1261 ccgttaaggt cgccgcaagc gaagggacgt cacgctccat cccgctcggg atcggcaaat
     1321 cggttgcgat cgaccttccg acggatatca aggacgtgct ggttgccgat ccgaagattg
     1381 ccaacgcggt cgtccgctcg tcgcgacggg tctacatgat cggtgttgcg gtaggacaaa
     1441 cgaatatttt ctttttcgac gccaacggca aacaaattgc ggggttcgac atcgcggtga
     1501 cccgcgacct caatggcgtg cgcgccgctc tcaagcagtc gatgcctgat tccgacatcc
     1561 atatcgaggg tgtcggaacg gacggcatca tgctgtccgg caccgcagcc agcgccgccg
     1621 aatcccagca tgctttcgat attgccgcgc ggctcgtcgg cggtgaaacg aaggtcgtaa
     1681 acggtattgc ggtccgcggg cgggatcagg tgatgctcaa ggtgaccgtc gctgaagtgc
     1741 ggcgcgacgt catcaagcag ctcggcatcg atctatcggg aagtctcaac tacggcactg
     1801 ctgtggtgaa tttcaacacc agcaatccct tctcggccac tggtcagccc ataagcgata
     1861 cgtttttccg cggcgcgttc ccgagcgtta cggccacgct gcgtgcgatg gagcgcgcgg
     1921 gcgtgatccg caccctggca gagcccaacc tgaccgcgat ttccggagag acggcgacat
     1981 tcgttgccgg cggtgaattc ccgatcccga acggactgtc gtgcgacacg actcgttcgc
     2041 cgccgatctg ccaaccccag atcacattca agaaattcgg tgtgagcctg gtgttcaccc
     2101 ccgtcgttct ttcggaaggg cgcatcagcc tgaaggtcat gacggaagtg tccgatcttt
     2161 caaccgagaa cgcgatgacc ctgcaggtca caggatccgc caactcgttg accgtgccgt
     2221 cgatccagac gcgccgcgcc gacacggtgg tcgaaattcc gtcgggcggc tctctggcca
     2281 tggcgggcat gattcaggaa cagacgaaac aatcgatcag cggcttgccg gcgcttcaac
     2341 aggtgccggt gctgggcgct ttgttcagaa gtcgcgacta catcaatcac cagtccgagt
     2401 tgatggtaat tgtcacgcct tacgtggtgc gcgcggtagc gcaaaagcag ctatcgcgtc
     2461 cggatgacgg ctttgccgac ccgagcgacc cgcagaccgt cctgctcggc aacctcaacc
     2521 gcatctacgg cgtcgccggc aagtccgaca cgctgcgcgg caacaactat cacggcaagt
     2581 acgg
//
LOCUS       DASSRL010000322         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_918677, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000322 DASSRL010000000
VERSION     DASSRL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918677"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..577)
                     /locus_tag="VFL49_06590"
     CDS             complement(257..577)
                     /locus_tag="VFL49_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET7911678.1"
                     /translation="MAARSKPRIHIHPGEILKEEFLVPLGISANRLAGSLGVPANRIS
                     AIVNGDRGITAETAILLGRALRTTPEFWINLQAHYDLELARSTISADRVRSAERLAKE
                     LRRA"
     gene            complement(591..872)
                     /locus_tag="VFL49_06595"
     CDS             complement(591..872)
                     /locus_tag="VFL49_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7911679.1"
                     /translation="MIRSFGDKATAALFHGRGCDPRWQRFERVALRKLLMVNAAASLH
                     DLKSPPNNRLEALKKDRLGQHAIRINGQYRVCFVWSEGHAEQVEIVDYH"
     gene            complement(1076..1237)
                     /locus_tag="VFL49_06600"
     CDS             complement(1076..1237)
                     /locus_tag="VFL49_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911680.1"
                     /translation="MKLANAHQELDEAVAAYGWPADLCDEEILKRLFDLNQERATKQG
                     AEARNKAAK"
     gene            1428..2582
                     /locus_tag="VFL49_06605"
     CDS             1428..2582
                     /locus_tag="VFL49_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET7911681.1"
                     /translation="MEPMTVSEANPARRQIDDLAFQLNRDANRLAGRLAPAVAASIGD
                     LVRSMNCYYSNLIEGHDTHPIDIEKAMAKDFSADPEKRDLQQEAVAHIKVQAWIDSGQ
                     DAAIQGLVALTSEIHRRFCAELPERLLRLELPSGKTVKIVPGAWRTLGVAVGRHVPVS
                     PAAIPRFMGRWEEAFALPPARLLSELGSVHHRLVWIHPFADGNGRASRLLTHAILRRI
                     GVGSPLWAVSRGLARNVERYKALLQAADEPRRGDLDGRGALSEAALADFNRFFLEQAL
                     DQVSFMGELIEVDRLLARILLHVREEAALGRLDTGAEIVMRALFQSGELPRGEVDRLL
                     GTSPRTRTRILKALFDLKMLQADSHRAPIRLLFSAANAERWMPGLFPPKR"
     gene            complement(2617..2805)
                     /locus_tag="VFL49_06610"
     CDS             complement(2617..2805)
                     /locus_tag="VFL49_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911682.1"
                     /translation="MVRAESRVNVITINLRNALKYQAEYRPTRFDEEILKRLFDLNQE
                     RARAQGAAASRKKRDAGD"
     gene            complement(2882..3622)
                     /locus_tag="VFL49_06615"
     CDS             complement(2882..3622)
                     /locus_tag="VFL49_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HET7911683.1"
                     /translation="MPTFSDYTIFVDESGDHSLNVIDRDYPVFVLSFCIFRKSHYGQV
                     LCPRVQDFKFRHFGHDHVILHEHAIRKQLRPFLFLKSVDKRKDFLDQLTAIIADTEFV
                     LVSAIIDKRALARRYVTPGSPYDIALLFCMERAHAFLRGVGQAHLVTHIVVECRGAKE
                     DAALELQFRRICAGTSFEGKMDGFDIVFADKKANLPGLQIADLTARPVGRHVINPTQP
                     NRAFEILQTKFRRSPTGKIEGWGMKVFP"
     gene            complement(3622..>4895)
                     /locus_tag="VFL49_06620"
     CDS             complement(3622..>4895)
                     /locus_tag="VFL49_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HET7911684.1"
                     /translation="VATNSIGGGKNRMVLDAITRDSTIFEAWSDEPWVLDGAAVRVAL
                     ICATSKASNLASKLNGHDVSQIHPDLTGRSSAGDGEDLSKAKRLTENRKICFVGTVKA
                     GRFDIPGAQARALLAAPTNPNGQPNSDVIVPWFNGLDVVRRPQDKWIIDFGVNEDLSK
                     VELYEAPFQIVQQMVKPERSKVRRARYRDFWWLEAEPCVGMRDAIGRLKRFIAVPALA
                     KHRVCVWLSSTIQPDHQLMAIARDDDTSFGILQSRFHQLWSLRMGTSPEDRPRYTPST
                     CFETFPFPAGLTPNIPAKNYADDPRARRIAAAAKRLNELREAWLNPPDLVRREAEVVP
                     GYPDRILPKDAKAAAILKKRTLTNLYNERPAWLANAHQELDEAVAAAYGWPADLSDEE
                     ILKRLFDLNQERARARGEGKPAKSKAVPEEA"
BASE COUNT          888 a         1502 c         1491 g         1014 t
ORIGIN      
        1 atcaccaccg ccgccggcat caccgcgatg gtcgacctga tcttcgacgg caagctgccc
       61 agccggggct tcatccgcca ggagcagtgc gacatgggcg acttcctggc gaaccgcttc
      121 gggcggtact acgcgtgagc ggggtcaaga actgcgacag cgacggtgat tgagcgcggc
      181 tgccgccgtt gctatcgcag gctggtcaat gggaagcggc gcggcactgg aaggccgcca
      241 tccacccgta acagtgctac gcccggcgga gctccttcgc caggcgttcg gcgcttcgta
      301 cgcggtcggc cgaaatggtg cttctggcga gttccaggtc gtagtgcgcc tgcaggttga
      361 tccagaactc aggcgtggtc cggagcgccc ggccgagcag gatcgcagtt tcggccgtga
      421 tgccgcggtc gccgttgacg atggcggaga tccggttggc gggaacgccg agcgagccgg
      481 cgagacgatt ggcgctgatc ccgagcggaa ccaggaactc ctccttgagg atctcgccgg
      541 ggtgaatatg gattcgaggc ttgctgcgtg ccgccacggc tgcgctcctt tcaatgatag
      601 tcgacgatct caacctgctc cgcatggcct tcgctccaga caaagcagac ccggtactga
      661 ccgttgatcc ggattgcgtg ctggccaaga cggtcctttt tcaaggcttc gagccggttg
      721 ttcggcggag acttgagatc gtgaaggctc gccgcggcat tcaccatcaa cagcttcctc
      781 agcgcaaccc gctcgaaccg ctgccatctc gggtcgcaac cccttccatg aaagagggcg
      841 gccgtcgcct tgtctccgaa cgacctgatc atcgtcgccc ttcttacgtc ttacgtcaat
      901 cgtaatataa gttttcccca gtgtatcaac agatgcattg atatgccata acactaccgt
      961 atggattatt gatgagaagc gacggcggcg gcgaggacca ggggttcttg gccggtgcga
     1021 agcttctgca tgcgccggcg cgcttttgtt tgcggctggc ccgattggct ttaccctact
     1081 tcgccgcctt gttcctggct tccgcacctt gcttcgtcgc gcgctcctgg ttgaggtcga
     1141 acaggcgttt caggatttcc tcgtcgcaga ggtcggcggg ccagccgtag gcggcgaccg
     1201 cctcgtccag ctcctggtgc gcattggcga gcttcatcgg gcagactatg gcagccgctc
     1261 gcccagaatc gcgccatata acacgccaac actgccatat tgcgttcgac tgtgttttga
     1321 tttatgcatg cgaatcatca atttagtatc tgtataaaca cgccatgaaa cgcgtcagga
     1381 accatgcgaa acctgcgccc gtccgaggtg acgaaccggt cgccctgatg gagccgatga
     1441 cggtctccga ggcgaacccc gcccggcgcc agatcgacga ccttgccttc cagctcaacc
     1501 gagatgcgaa ccgcctggcc ggccgcctgg cgccggcggt ggccgcgtcg atcggcgacc
     1561 tcgtgcgttc gatgaattgc tactactcca atctgatcga aggccacgac acgcacccga
     1621 tcgatatcga gaaagcgatg gccaaggatt tctctgccga ccccgagaag cgggatctgc
     1681 agcaggaggc cgtcgcccac atcaaggtcc aggcctggat cgattcgggc caggatgcgg
     1741 cgatccaggg actggtggcg ctgacgtccg aaatccatcg ccgcttctgc gcggagctgc
     1801 cggagcggct tttgcggctg gagctgccat cgggcaagac cgtgaagatc gtgccgggcg
     1861 catggcggac tctcggcgtg gccgtcgggc gtcatgtccc ggtttccccc gccgcgatcc
     1921 cgcgcttcat ggggcgttgg gaggaggctt ttgccctgcc gccggcacgc ctgttgtcgg
     1981 aactgggctc ggtgcatcac cggctggtct ggatacaccc cttcgccgac ggcaacggcc
     2041 gcgcttcccg gctgctgacg catgcgatcc tgcgccgcat cggagtcggc agcccgctct
     2101 gggcggtgag ccgcgggctg gcgcgcaacg tcgaacgcta caaggcgctg ctgcaggcgg
     2161 ccgacgagcc gcgccgcggc gaccttgacg ggcgcggcgc gctgtccgaa gcggcgctcg
     2221 ccgacttcaa ccggttcttt ctcgaacagg cgctggatca ggtctcgttc atgggcgagc
     2281 tcatcgaggt cgatcgattg ctggccagaa tcctgctgca tgtgcgcgaa gaggcggcgc
     2341 tgggcaggct cgataccggc gccgagatcg tcatgcgcgc gctgttccag agcggcgagc
     2401 tgccgcgggg cgaggtcgac cggctgctcg gcacctcgcc ccgcaccagg acgcggatat
     2461 tgaaggcgtt gttcgatctc aagatgctgc aggcggattc gcatcgcgcg ccaatacgcc
     2521 tgctgttctc ggcggcgaat gccgagcgct ggatgcccgg actgtttccg ccgaagcgct
     2581 gagcacgaag cgccggcaac gccgatcagt cgccgatcag tccccggcgt cgcgtttctt
     2641 cctgctcgca gccgcgccct gcgccctcgc ccgctcctgg ttgaggtcga acaggcgttt
     2701 caggatttcc tcgtcgaacc gcgtcgggcg gtactccgcc tgatatttca atgcattacg
     2761 caagtttatc gtaatgacgt tgacacgcga ctcggcacga accatcttgg agcgagaaat
     2821 ggactggggg ctgttcccgg tcggcgctgg gccttggtgt atgccgaggc ctttcgcttt
     2881 tctagggaaa cactttcatt ccccatcctt cgattttgcc ggtcgggctg cgcctgaatt
     2941 tcgtctgcag gatttcgaac gctcggttcg gctgagtggg gttgatcacg tgccgaccca
     3001 ccggccgcgc cgtcaaatca gcgatctgca atccgggtag attggccttc ttgtcggcaa
     3061 acacgatgtc gaagccgtcc atttttcctt cgaagcttgt gccggcgcag atgcgcctga
     3121 attgcagctc caatgccgcg tcctctttcg cgcctcgaca ctcgacaacg atgtgggtca
     3181 cgagatgcgc ctggccgacg cctctgagaa atgcatgcgc acgttccatg cagaacagca
     3241 gcgcaatgtc gtaggggctg cccggcgtga catagcgccg tgccagggcg cgtttgtcga
     3301 tgatcgcgga aaccaacaca aactcggtat ccgcgatgat cgccgtcagt tggtcgagga
     3361 aatccttgcg cttgtccaca ctcttcagga acagaaaggg gcggagttgc ttgcggatag
     3421 catgctcgtg cagaatcaca tgatcatgcc cgaagtgacg aaacttgaaa tcctgcacgc
     3481 gcggacaaag cacttgtccg taatgcgact tgcgaaagat gcaaaaactg aggacgaaga
     3541 ccgggtagtc ccgatcgatg acgttcaagc tgtgatcgcc actctcatcg acgaaaatag
     3601 tatagtcgct gaaggtcggc attacgcttc ttccggaacc gcttttgact tggccggctt
     3661 gccctcgcct cgcgccctcg cccgctcctg gttgaggtcg aacaggcgtt tcaggatttc
     3721 ctcgtcggag aggtcggcgg gccagccata ggcggcggcg acggcctcgt ccagctcctg
     3781 gtgcgcgttg gcaagccagg ccgggcgctc gttgtagagg ttggtcagcg tgcgcttttt
     3841 gaggatcgcc gccgccttcg catccttcgg gagaatgcgg tcgggatagc ccggcaccac
     3901 ctcagcctcg cggcgcacga ggtcgggcgg gttgagccac gcctcgcgca gttcgttcag
     3961 ccgcttcgcc gccgccgcga tccgccgcgc gcgcggatca tcggcgtagt tcttggccgg
     4021 gatgttgggc gtcaggcccg cggggaaggg gaaggtctcg aagcaagtcg aaggggtgta
     4081 gcgaggacga tcctccgggc tcgtgcccat gcgcagagac cacaactgat ggaatcggct
     4141 ttgtagaatt ccaaagctgg tgtcgtcgtc gcgagcaatt gccattaact gatgatctgg
     4201 ttgaatcgta gagctaagcc aaacgcaaac gcgatgtttg gccaacgcag gcacagcaat
     4261 gaatcgcttc aatcgtccga tggcatctcg catacctacg caaggctctg cttcaagcca
     4321 ccaaaaatcg cgatatcgtg cgcgacgtac cttggaacgc tccggcttaa ccatctgctg
     4381 cacaatttga aagggcgctt cgtataactc cacttttgaa aggtcttcgt tcaccccaaa
     4441 atcgattatc cacttgtctt gtggacgcct tacgacatcc aatccgttga accacggcac
     4501 tataacgtcg gaattcggct gaccattggg attggtggga gcagctaaca gtgctcttgc
     4561 ttgtgctcct ggtatgtcga accgtcctgc ctttacagta ccgacgaagc atatcttccg
     4621 gttttcagta agccgtttcg cctttgataa atcttcgcca tcgcctgcgc tgcttcgacc
     4681 tgtgagatca gggtgtattt gggagacatc gtgaccgttt agcttcgacg caagattgct
     4741 cgcctttgag gtagcgcaga tcagcgcgac acgcaccgct gctccatcga gcacccacgg
     4801 ttcatctgac cacgcctcga agatggtcga gtctcgtgtg atcgcatcaa gaaccattcg
     4861 gtttttcccg ccgccaatag aattagtagc aacca
//
LOCUS       DASSRL010000323         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_131117, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000323 DASSRL010000000
VERSION     DASSRL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131117"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFL49_06625"
     CDS             <1..352
                     /locus_tag="VFL49_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HET7911685.1"
                     /translation="NVTRFPYEIARLAQDIAGGLVVTMPSEKDLQNNEIGPLIRKFLQ
                     GRDGVEVTDRMRILRLIENMTIGRNAVGYLTESLHGAGSPQAQRIQIARGMKIEEKKA
                     RARHLAGVAQTGSK"
     gene            complement(334..714)
                     /locus_tag="VFL49_06630"
     CDS             complement(334..714)
                     /locus_tag="VFL49_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HET7911686.1"
                     /translation="MGVNKKHDEFHTLDMNAGWETPSGYPKGIEQKILSGALDETNRR
                     GTRTRLLRFAPGVFTTKPFVHEYWEEVYLVSGDLTVGNDEQGNGGKSFPPNTYAVRPP
                     GAYHGPFKSDGGCMLLEIHYFDPV"
     gene            complement(721..1251)
                     /locus_tag="VFL49_06635"
     CDS             complement(721..1251)
                     /locus_tag="VFL49_06635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7911687.1"
                     /translation="MLDTRRFRDALGQFPTGVTIITALTAGGDRLGMTISSFNSLSLD
                     PPLVLFSILRKANSFAAWQQAKRYAINVLNESQEELSNKFARPLGEKWIGQTVLTGKT
                     GLPLLPNAVIAFECEAYGRHDGGDHEIFVGRVVELHENQAKRGHPLVFFGGQYRRLGA
                     VEADAPADIVNVLHGW"
     gene            1321..2001
                     /locus_tag="VFL49_06640"
     CDS             1321..2001
                     /locus_tag="VFL49_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HET7911688.1"
                     /translation="MLVFERHSNGETDRAELVPKALIVAGWTGRDEKALRHHIEELAA
                     IGVPRPSSVPVFYRISTANLMQAAQLEVLGPDTSGEAEPVIVAMHDGLWLGVGSDHTD
                     RKAETMGIALSKQLCAKMVGKTLWRLDEVVGHWDQLILRAEVMIGGRRLKYQEGPLSA
                     MRNPSDLMSRIGGEAKFAAGTIMFCGTLGAIGGIRPAAQFTALLEDPVLGREMRCDYR
                     IDALPVVR"
BASE COUNT          390 a          613 c          636 g          389 t
ORIGIN      
        1 caatgtcacg cgctttccct acgagatcgc gcggctggca caggatatcg ccggcggcct
       61 tgtcgtcacc atgccgtcgg agaaggacct gcagaacaac gaaatcggtc cgttgatccg
      121 caagttcctg caagggcgcg atggcgtcga ggtcacggac cgcatgcgca tcctgcggct
      181 gatcgagaac atgaccatcg gccgcaacgc cgtcggctat ctgaccgaaa gcctgcatgg
      241 cgccggctcg ccgcaggcgc aacgcatcca gatcgcgcgc ggcatgaaga tcgaggagaa
      301 gaaggcgcgc gcccggcacc tcgccggcgt cgctcagacc ggatcgaagt agtgaatttc
      361 caggagcata cagccgccgt ccgacttgaa tggcccgtgg taggcgcccg gcggacggac
      421 ggcataggtg ttgggcggaa atgatttgcc gccgtttccc tgttcgtcat tgccgacggt
      481 gaggtcgccc gacacgaggt aaacttcttc ccaatattcg tgcacgaatg gcttcgtcgt
      541 gaatacgccg ggtgcaaagc gcaggagccg cgtgcgcgtg ccgcgcctgt tggtctcgtc
      601 cagcgcgccg gacaaaatct tctgctcgat gcctttcgga tagccggacg gcgtttccca
      661 gccggcgttc atgtcgaggg tgtggaattc gtcgtgtttc ttgttcacgc ccatggcgct
      721 tcaccatccg tgcaggacat tgacaatgtc ggcgggcgcg tccgcctcga cggcgccaag
      781 acgccggtac tggccgccga agaacaccag cgggtggccg cgcttcgcct gattttcatg
      841 cagctcgacc acgcgtccga cgaagatctc gtgatcaccg ccgtcgtggc ggccgtaggc
      901 ttcgcactcg aaggcgatca cggcgttcgg caacaatggc aggccggtct tgccggtcag
      961 caccgtctgc ccgatccatt tttcgcccag cggacgcgcg aacttgttgg acagctcctc
     1021 ctggctctcg ttcaggacgt tgatcgcgta gcgcttggcc tgctgccagg cggcgaaact
     1081 gttggccttg cgcaaaatac tgaacaggac gagaggcgga tcgagcgaca gcgaattgaa
     1141 cgaactgatg gtcataccaa gccggtcgcc gccagccgtc agcgctgtga taatagtgac
     1201 gccggtcgga aactgaccaa gcgcgtcacg gaaacgtcgg gtgtccaaca cggatttccg
     1261 ccggtgaatt ttcttcagtg atcatgtacc aagtgcctgt cgccaatcaa ggaacaagcc
     1321 atgctcgttt tcgagcgcca ttcaaacggt gaaactgatc gcgccgaact tgtgccgaaa
     1381 gcgctgatcg ttgcgggctg gaccggccgc gacgagaaag cgctgcgtca tcacatcgag
     1441 gagcttgcgg cgatcggcgt accccgccca tcgtcggtgc cggtctttta ccgcatcagc
     1501 acggcaaatc tcatgcaagc cgcgcaactg gaggttcttg ggcccgacac gtccggcgag
     1561 gcagagccgg tgatcgtcgc gatgcacgat gggttgtggc tcggcgttgg ttccgatcac
     1621 accgaccgca aagcggaaac gatgggcatc gcgctatcga aacagctctg cgccaagatg
     1681 gtcggcaaaa ccctttggcg tctcgacgag gttgtcgggc attgggacca attgatcctg
     1741 cgagccgagg tgatgatcgg cggcaggcgc ctgaaatacc aggagggccc gctgtcggcc
     1801 atgcgcaatc cctccgatct gatgtcacgc atcggcggtg aagcaaaatt tgctgcgggt
     1861 acgatcatgt tctgcggaac gctcggcgcc atcggaggca ttcgcccggc ggcacagttt
     1921 actgctctgc tcgaggatcc ggtcctcggc cgcgagatga gatgcgacta ccggattgat
     1981 gccttgccgg ttgtccgctg atgtggatca accggtcgca ttcgataa
//
LOCUS       DASSRL010000324         4845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1093643, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000324 DASSRL010000000
VERSION     DASSRL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093643"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFL49_06645"
     CDS             <1..883
                     /locus_tag="VFL49_06645"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET7911689.1"
                     /translation="ELAIKHGKAVRIGANWGSLDQELLTAMMDENAKSARPFDARAVT
                     REALVRSALLSAARAEDLGMKREQIILSAKVSAVQDLIAVYTELARRCAYALHLGLTE
                     AGMGSKGIVASSAAMGVLLQQGIGDTIRISLTPEPNGDRTREVQVAQELLQTMGLRTF
                     VPLVAACPGCGRTTSTTFQELAAEIQGFIRESMPEWKKRYPGVETLNVAVMGCIVNGP
                     GESKHADIGISLPGTGEQPTAPVFIDGKKAATLRGPALTTDFKQMVVDYIERRWGAGP
                     APAKKLADIAGEFSHSA"
     gene            938..3310
                     /locus_tag="VFL49_06650"
     CDS             938..3310
                     /locus_tag="VFL49_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7911690.1"
                     /translation="MTSLPPADRDVKVPPRAVKPRNLALGIERIGFFALSHRTLCAIV
                     FIILSAVAAVGFVRVKVDDSLSQLFRSDTAEFRTYEEVTRRFPSNEFDVLIVIEGERL
                     LERASLEKLRELAIELQLIDGTRGLISIFSAREPPDNGLIPPPLFPNPLPQGADYQAL
                     IKRVTSNEIIRGKMLSDDGKLTLMVLALEPSSTAPDKLGPTVAAIRDTVKTHLQGTGL
                     KAELSGVPVMQLEIRNAIQRDRLVYNTLGFAVGCLIAIIFFRRPSFMIIAAAPPLLAI
                     LMALGALGWLGFSLNMFLNVMTPLIMVISFSDSMQITFAARDRLLLGDDKIQAFRNAL
                     LIVGPACVLTHATAGVSFIALQFSSSDLIRTFGEAGLIAVAVALIAVLMGVPLLGVLL
                     IRNEAAFVAAVRDMDTALDWLRRLCSWIAIRMVARPGLYSLVSLIVVVVLSLGYMQLQ
                     PRYRLADQVPDRQQAVLASQRLDVKLTGANPFDVYIQFPKGASLYDPDTLAVIADVHA
                     LLERQKGIGNVWSLETLRRWLAEKLHKSDVATLKQYVDLLPPYLTRRFISAEQDAVIV
                     SGRVPDSDASQLLPIVDRIDQQLNAVRAKYPGYKIAVTGLSVISARNSALMIDKLSDG
                     LTIEIVFVAAFIGLAFWSPVVMLISILPGLFPIVLAGCMLLLTGQGLQFASVIALTVS
                     FGLGLSATIHFLNRLQIEDVDPDPAVGVERATILVGPPLILTSAVLACGLAMTVLSSL
                     PSLRLFGWLSAFAMVAALLADMFMLRPIAMFLSKLVRRTSRRSLLRSSAE"
     gene            complement(3321..4217)
                     /locus_tag="VFL49_06655"
     CDS             complement(3321..4217)
                     /locus_tag="VFL49_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911691.1"
                     /translation="MVLEHFQADFPDALPVLLQASQNTKRIGNCVFAEPGRIRRAGGL
                     FFRRALERVQRLWSRKAFLVGGEGRGAANLNQDNGCADFGVHCVRPDFRAWFHLSTAA
                     VCSRSTENPDMLGRYCVGFRSSSRSTPSSRSRWLKKLAGMAALSLSLAVCIADSSRAQ
                     SIAPFNRLAGQWAGSGTIELANGRRESIKCRAAYDVLSQQKKLQLNIRCASDSYNFDL
                     RASATYSGGSVSGIWSESTRNAGGTISGRAEGDHIHVLAKSSSFAATLTVVTHGSRQS
                     VVIRTQNADASIKGVSMNLRRS"
     gene            complement(4227..>4845)
                     /locus_tag="VFL49_06660"
     CDS             complement(4227..>4845)
                     /locus_tag="VFL49_06660"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7911692.1"
                     /translation="RSAIDKVMMERCIGLSAKGVEQGEMPFAGMICRDADVLVETSNQ
                     VSRTEDITRHAEMVAISKTQELLDRRDLSDCTLYSTVEPCAMCAFAARETRIGRVVYA
                     IKSPMMGGVSKWNVLRDTELSNAMPEVFGPVPEVVAGLMQDAAEQVWQAWNPLIWAVI
                     KHRGYLGGIDAGCTTLHAIPDRHGLLRRLFTFHNHAPDLFRKLRF"
BASE COUNT          907 a         1531 c         1442 g          965 t
ORIGIN      
        1 cgaactcgcc atcaagcacg gaaaggccgt tcgtatcggc gcgaactggg ggtcgctcga
       61 tcaggagctg ctcaccgcga tgatggacga gaacgcgaaa tcggcgcgtc cattcgatgc
      121 gcgcgccgtc acgcgcgagg cgctggtgcg ctcggcgctt ctgtccgcgg cgcgcgcgga
      181 agatctcggc atgaagcgcg agcagatcat tctctccgcg aaagtctcgg cggtgcagga
      241 tctgatcgcg gtctataccg agcttgcgcg gcgctgcgcc tatgccctgc atctcgggtt
      301 gaccgaagcg ggcatgggtt cgaagggcat cgtcgcctcg tccgcggcca tgggcgtgct
      361 cctgcagcag gggatcggcg acacgatccg catttcgctc acgccggagc cgaacggcga
      421 tcgcacgcgc gaagtccagg tcgcgcagga gctgctgcag acgatgggcc tgcggacatt
      481 cgtgccgctc gtggcggcct gcccggggtg tggccgcacg acctcgacca cgttccagga
      541 actggcggcc gagattcagg gcttcatccg cgagtcgatg ccggagtgga aaaaacgcta
      601 ccccggcgtg gaaacgctca acgtcgcggt gatgggatgt atcgtgaacg ggcccggcga
      661 gagcaagcat gccgacatcg gcatctcgct gccgggcacc ggcgagcaac ccactgcgcc
      721 tgtgttcatc gatggcaaaa aagccgccac cctgcgtgga ccggcgctca ccaccgattt
      781 caagcagatg gtcgtcgact acatcgagcg ccgctggggc gcaggacccg cgccggcaaa
      841 gaaacttgcc gatatcgcag gagaattttc gcactcggcc tagtggaaat ttccagtctg
      901 aaagtccatc cttcaccgcc atctggaccg agtatctgtg accagccttc cgccagccga
      961 ccgcgatgtg aaagtcccgc cgcgggcggt aaagccgcgg aatcttgcgc tgggcatcga
     1021 acgcatcggc tttttcgcac tgtcgcaccg gaccctgtgt gcgatcgtgt tcattatcct
     1081 aagcgcggtt gcggcggtcg gcttcgtgcg ggtcaaggtg gacgattccc tcagccagct
     1141 atttcgctcc gacaccgccg aattccggac ctacgaagag gtcacgcgcc gctttccctc
     1201 caacgaattt gacgtgctga tcgtcatcga gggtgagcgt ctgctggaac gtgcgtcgct
     1261 cgaaaagctg cgtgagctcg cgatcgagct tcaactgatt gacgggacgc gcgggctgat
     1321 ctccatattc tccgcgcgcg agccgcccga caacgggctg atcccgcctc ccctttttcc
     1381 aaacccgctt ccgcagggcg ccgattacca ggcgctgatc aagcgggtaa cgtcgaacga
     1441 gatcatccgc ggcaagatgc tgtcggacga cggcaagctc actctgatgg tgctcgcact
     1501 cgagccgtca tccacggcgc cggacaaact gggaccgacc gtcgcggcaa tccgcgacac
     1561 ggtgaaaacc catctgcagg gcaccgggct caaagccgag ctctccggcg tgccggtgat
     1621 gcagctcgag atccgcaacg cgatccagcg cgaccggctg gtctacaaca cgctgggatt
     1681 cgcggtcggc tgcctgattg cgatcatctt cttccgccgc ccgtcgttca tgatcatcgc
     1741 ggcggcaccg ccgctgctgg cgatcctgat ggcgctcggc gcgcttggct ggctgggatt
     1801 cagcctgaac atgttcctga acgtgatgac gccgctgatc atggtcatca gcttctcgga
     1861 cagcatgcag atcacgttcg cggcgcgcga ccggctgctt ttgggcgacg acaagattca
     1921 ggcgttccgc aatgcactgc tgatcgtggg gcccgcctgc gtgctcaccc acgcgaccgc
     1981 aggtgtgtcg ttcatagccc tgcaattttc gtcttccgat ctcattcgca ccttcggcga
     2041 agccggcctc atcgccgtcg cggtcgcact catcgcggtt ctcatgggcg tgccgctgct
     2101 tggcgtcctg ctcatccgca acgaggccgc atttgtcgcg gcggtcaggg atatggacac
     2161 ggcgctcgac tggctgcgcc ggctgtgcag ctggatcgct atccgcatgg tcgcgcggcc
     2221 gggtctctac tcgctggtga gcctgatcgt cgttgtcgtg ctctcattgg gctatatgca
     2281 actgcagccg cgctatcggc ttgcagatca ggtgccggac cggcagcagg cggtgctggc
     2341 cagccaacgt ctcgacgtca agctgaccgg cgcaaatccc ttcgatgtct atatccagtt
     2401 cccaaagggc gcatctcttt atgatcccga cactctggcc gtaattgccg acgtccacgc
     2461 tctgctcgaa cgccagaagg ggataggcaa cgtctggtcg ttagagacgt tgcgtcgctg
     2521 gctcgccgaa aaattgcaca aatccgacgt cgcaacgctc aagcaatacg tggacctgct
     2581 gccgccctat ctgacgcgcc ggttcatttc ggcggagcag gatgccgtca ttgtcagcgg
     2641 gcgtgttccg gattccgacg cgagccagct gctgccgatc gtcgaccgga tcgatcagca
     2701 gctcaacgca gtgagggcaa aatatcccgg gtacaagatt gcagtgaccg gcctgtcggt
     2761 catatcggcg cgcaacagcg ccctgatgat cgacaaactg agtgacggcc tcaccatcga
     2821 gattgtcttc gtggccgcgt tcatcggcct ggcgttctgg tcacccgtcg tcatgctgat
     2881 cagcattctg cccggacttt ttccgatcgt ccttgcgggt tgcatgctgc tgctgacggg
     2941 ccagggcctg caattcgcga gcgtcatcgc gcttacggtc tctttcgggc ttggcctgag
     3001 cgcgacgatc catttcctca accgcctgca gatcgaagac gtcgatcccg atcccgccgt
     3061 gggcgtcgaa cgcgccacaa tactggtcgg accgccattg atcctcacgt ctgcggtgct
     3121 cgcgtgtggt ctggcgatga ccgtgctgtc gagcctgccg tcactgcggc ttttcggctg
     3181 gctcagcgct tttgcgatgg tggcggcact gctcgcagac atgttcatgc tgcgcccgat
     3241 cgccatgttc ctcagcaagc tcgtccgccg cacgtcgcgg cgatcgctgc tgcgttcttc
     3301 ggcggaatag agcgcggctg tcagctgcgc cgcagattca tcgacacgcc tttgatgctg
     3361 gcatcggcgt tctgcgtcct gatcacaacc gactgacgcg acccatgcgt gacgactgtc
     3421 agagttgcgg caaaagatga actcttggcc agcacatgaa tatgatcgcc ttcggctctt
     3481 ccggagatcg tgcctccggc gtttcgcgtc gattcactcc aaatgccgga aaccgaaccg
     3541 cccgagtaag tggcactcgc gcgcaaatcg aaattgtagc tgtcgctggc gcagcggata
     3601 ttgagttgca attttttctg ctggctgagc acatcgtagg cggcgcggca tttgatcgac
     3661 tcgcgcctgc cgttcgccag ttcaatggta ccggagccgg cccattgacc tgccaaacgg
     3721 ttaaatggtg ctatgctttg cgcgcgggac gaatctgcaa tgcaaacagc gagcgaaaga
     3781 gacagcgcgg ccatgcccgc caacttcttc aaccagcgtg atcgcgatga cggcgttgaa
     3841 cgcgacgaac tcctaaagcc gacgcagtaa cgaccgagca tatccgggtt ctctgtagaa
     3901 cgtgaacaca ctgccgcggt tgacaaatga aaccatgcac gaaaatcggg cctgacacaa
     3961 tgtacgccaa aatctgcgca tccattgtct tgattcaaat ttgcggcgcc gcggccttcg
     4021 ccgccgacga gaaaagcctt ccgggaccac agcctttgaa cccgttccag ggcacgccgg
     4081 aagaacagtc cgcctgcgcg ccggatgcga cccggttctg caaagacgca attcccgata
     4141 cgtttcgtgt tctggcttgc ctgcaagagc accggcagcg catccggaaa gtctgcctga
     4201 aagtgctcga ggaccacggg caataactaa aaccgcaatt tccggaaaag atccggtgcg
     4261 tggttgtgga acgtaaagag ccggcgtaac agcccgtgcc ggtccggaat ggcgtggaga
     4321 gtcgtgcaac cggcgtcgat accgccgaga tatccgcgat gcttgatgac ggcccagatg
     4381 agcggattcc atgcttgcca gacctgttcg gccgcatcct gcatcaaccc ggcgaccact
     4441 tcagggaccg gaccaaaaac ttccggcatt gcgttggaaa gctcggtatc ccgcagaacg
     4501 ttccattttg acactccgcc catcatgggc gatttgatgg cgtagacaac gcgtccgatg
     4561 cgcgtctccc tcgcggcgaa tgcgcacatc gcgcagggct caacagtcga atagagcgtg
     4621 caatccgaca gatccctgcg gtcgagcaac tcttgcgttt tcgagatcgc aaccatctcg
     4681 gcgtgacgcg ttatatcttc ggttcgcgag acttgattgc tcgtctcaac cagaacgtcc
     4741 gcatcgcggc aaatcatgcc cgcaaatggc atctctccct gctcgacccc tttcgcagaa
     4801 aggccgatgc agcgttccat catgaccttg tcgatggcag accgc
//
LOCUS       DASSRL010000325         7298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_831082, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000325 DASSRL010000000
VERSION     DASSRL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831082"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..751
                     /gene="aat"
                     /locus_tag="VFL49_06665"
     CDS             65..751
                     /gene="aat"
                     /locus_tag="VFL49_06665"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7911693.1"
                     /translation="MANRENTFIEITPDVLLKAYACGIFPMAESADDPALYWIEPDMR
                     GIIPLQGFHVPSRLARTVRSTLYTVHIDRDFDAVIKGCAEPKHDRARTWINIRIRRIY
                     RSLFDRGHCHTVEVYDGDHLVGGLYGVSLGRAFFGESMFHRMRDASKIALVHLVARLK
                     VGGYRLLDTQYVTEHLKTFGAVEIPKRRYHRLLEEALHSGADFAALPATVSGEEALAV
                     IAGTQRSRRH"
     gene            complement(783..1697)
                     /locus_tag="VFL49_06670"
     CDS             complement(783..1697)
                     /locus_tag="VFL49_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HET7911694.1"
                     /translation="MARRIRPTALFVLAVLLAAPPAFAQFGSIFSNPPPRPPADVPNA
                     PQSQQQPPPYYPSGRDTPPSQPLPAPMNLPPSNRPGAGSIQSQPLPPPPGGIVAPAPP
                     PGQRPQQDVSTPSQPPTALPPGPGSPRNAPQQTNTAPQPNDEVVVEPPPQRITNPTAI
                     FSGLDKITGRIISFDVAINETVQFGALQVTPRVCYSRPPTETPNTDAFVEVDEVTLQG
                     EIKRIFTGWMFAASPGLHAVEHPIYDVWLTECKGGRNPNVAEVPAEQTPKPPARQQQQ
                     QPQQRRTSPPPQPQRAPPPPGLFPPPQR"
     gene            complement(1815..2228)
                     /locus_tag="VFL49_06675"
     CDS             complement(1815..2228)
                     /locus_tag="VFL49_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HET7911695.1"
                     /translation="MKSFLLKLFTWWNSQTFGTQFWTWMYGEFVGEDEFGNRYYRTRG
                     AKIDKTLGFERRWVIYNGYTEASTVPPSWHGWLHHTVDTPPTQESYKPFPWQKPHRPN
                     LTGTPGAYRPPGSTLATGRRPKATGDYKAWNPGGQ"
     gene            2355..3491
                     /locus_tag="VFL49_06680"
     CDS             2355..3491
                     /locus_tag="VFL49_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET7911696.1"
                     /translation="MNSLGAALTQVKDLLDWLPDPVVALLILAIAVLIALVLHRWARK
                     LVRRALAGRYPYVFSVFTQTRNLTRLALLILAMIVAIPVAPLPPETAVLLARAMGVAV
                     IGLIGWAAIIALHIAADLYLRRFRLDIDDNLLARKHNTQVRVLSRTIDVLLIMLTLGA
                     ALMTFPAVRQYGVSLFASAGVAGIVAGLAARPVLSNLMAGVQLAMTQPIRLYDAVIVE
                     NEYGTVEEITSTYVVVKLWDLRRMIVPLTYFIEKPFQNWTRESSSLIGNVMIYLDYRA
                     PIDLIREKFAAILKESHRWDGQVSAVQVTDFKEGTMEVRLLMSARSSGQAFDLRCEVR
                     EKLIEFLQREHPEALPHTRQIAFRGDSGAAAGSENEGRRAAARR"
     gene            4255..>7298
                     /locus_tag="VFL49_06685"
     CDS             4255..>7298
                     /locus_tag="VFL49_06685"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET7911697.1"
                     /translation="MRIERRYTKDEQSAYADIAFRFTTSEIRNPDGSVVFKADNVEVP
                     EFWSQVASDVLAQKYFRKAGVPARLKKIEENSVPSFLWRSVADEDALAALPEKERYIG
                     ETSAKQVFDRLAGTWTYWGWKGGYFDSESDAQAFFDELRYMLAAQMCAPNSPQWFNTG
                     LHWAYGIDGPSQGHYYVDPFTGKLTKSKSAYEHPQPHACFIQSISDDLVNEGGIMDLW
                     VREARLFKYGSGTGSNFSLLRGEGERLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVIVDVDHPDIEQYIDWKVLEEQKVAALVTGSKINQKHLKAVLKACVNCEGSGDD
                     CFSPEKNPALKREIKLARRAMVPDNYIKRVIQFSKQGYKDIHFPTYDTDWDSDAYLTV
                     SGQNSNNSVRVTDHFLKAVEADGKWDLKFRKNGKVAQTLDARALWEKIGYAAWASADP
                     GLQYHTTVNDWHTCPASGEIRASNPCSEYMFLDDTACNLASLNLLTFRDEKTGEFQVE
                     SYEHGVRLWTVVLEISVMMAQFPSKQIAELSYEFRTLGLGYANIGGLLMTSGIPYDSD
                     SGRAICGTLSAIMTGVSYATSAEMAEKLGPFPGYKKNREHMLRVMRNHRRAAYGERTG
                     YEKVATPPVPLDHSACPDQTLIAHAKTAWDRALTLGEKHGYRNAQATVIAPTGTIGLV
                     MDCDTTGIEPDFALVKFKKLAGGGYFKIINRAVPEALRTLGYSEADIAEIEAYAVGHG
                     TLSQAPAINHTTLKAKGFTDEAIARIEKALPTAFDIKFAFNKWTLGEDFCRDKLGVDA
                     GDLASPAFDLLAALGFGKREIEAANIHVCGAMTVEGAPHLKAEHYPVFDCANPCGRTG
                     KRYLSVESHIRMMAAAQPFVSGAISKTINMPNEATVEDCKAAYLLSWKLALKANALYR
                     DGSKLSQPLNAQLIADDDDEDDAVEMFLDRPAAARATGLAEKVVEKIVERITVLRERE
                     RMPDRRKGYTQKAVVGGHKVYLRTGEYDDGRLGEIFIDMHKEG"
BASE COUNT         1386 a         2278 c         2246 g         1388 t
ORIGIN      
        1 caatcgagtg ggtttcgagg ccgctgttgt ttgcgctacc ataggacagg ctcgtcgctt
       61 cgccatggca aaccgcgaaa acactttcat cgaaatcacg ccggacgtgc tgctgaaggc
      121 ctacgcctgt ggaatttttc cgatggcgga aagtgccgat gaccccgcgc tctactggat
      181 cgaaccggat atgcgcggga tcattccact gcaagggttt catgttccgt cacgcctcgc
      241 ccgcaccgtg cgctcgacac tctacacggt ccatatcgac cgcgacttcg acgccgtgat
      301 caaaggctgc gccgaaccga agcatgaccg cgcgcgcaca tggatcaaca tacgcatccg
      361 ccgcatatac cgatcgctgt tcgatcgcgg ccattgtcat acggtcgaag tctacgacgg
      421 cgatcatctc gtcggcgggc tttacggtgt gtcgcttggg cgcgctttct tcggcgagag
      481 catgttccac cggatgcgcg acgcttcaaa aatcgcgctc gtgcacctgg tcgcgcggct
      541 caaggtgggc ggctacaggt tgctcgacac ccaatatgtt accgagcatc tgaagacgtt
      601 cggtgccgta gaaattccaa agcgccgcta tcaccgactg ctcgaagaag cgttgcacag
      661 cggtgctgat tttgcggctc tgcctgccac ggtcagcggc gaggaagcgc tcgcggtcat
      721 tgcgggaaca cagcggtcca gacgccactg agcagattgc acccgaccgg gaacggatat
      781 ccctaacgct gaggcggcgg gaacaagccg gggggcggcg gcgcccgctg cggctgtggc
      841 ggaggcgatg tgcggcgctg ctgcggttgc tgttgttgct gccgcgccgg cggcttcggc
      901 gtttgctcag ccggcacttc ggcgacgttc ggattgcgcc cgcctttgca ttcggtgagc
      961 cagacatcgt agatcgggtg ctcgaccgcg tgcaggcccg ggctcgcggc gaacatccac
     1021 ccggtgaaaa tccttttgat ctcgccctgc aacgtcactt cgtcgacttc aacgaaagca
     1081 tccgtattcg gcgtctcggt cggcggccgc gaataacaca cgcgcggcgt gacctgcagc
     1141 gcgccgaatt gtacggtctc attgatcgcg acatcgaacg aaatgatccg tccggtgatc
     1201 ttatcgagac cggagaatat cgctgtcggg ttggtgatgc gttgcggcgg tggctcgacc
     1261 acaacttcgt cattgggctg cggcgccgtg ttggtctgct gcggagcatt gcgcggcgaa
     1321 cccggaccgg gtggcagcgc ggtgggcggc tgcgagggcg tcgatacatc ctgttgcgga
     1381 cgctgacccg gcggcggagc cggcgcgacg atgccgcctg gcggtggcgg cagcggctgc
     1441 gactggatcg agccggcgcc cggccggttc gaaggcggca aattcatggg ggctggcaat
     1501 ggctgcgacg gcggcgtgtc gcgcccactc ggatagtaag gcggtggttg ctgctgcgac
     1561 tgtggcgcgt tcggaacatc cgcaggcggc cgcggcggcg gattgctgaa gatcgagccg
     1621 aattgtgcaa atgccggcgg cgccgcaagg agcacggcga gcacaaacaa cgcggttggg
     1681 cgaatgcggc gcgccatggt cagagatcag taatcagcgg tcagtaatca aggtcaagtg
     1741 ttcaggtttc gaacggaacc atggaaatca ggctttatca gggatcaggt gatacctaat
     1801 ctcctgatgc ctgattactg acctcccgga ttccaggcct tgtaatcccc ggtcgccttc
     1861 ggccggcggc cggtggcgag cgtcgatccc ggcgggcggt aggcaccagg cgttccggtc
     1921 aaattcgggc ggtgtggttt ctgccacgga aacggtttgt agctttcctg cgtcggaggc
     1981 gtgtcgacag tatgatggag ccagccgtgc caggacggcg gcacggtgga ggcctctgtg
     2041 tagccgttgt agatgaccca tcgccgctcg aagccgagcg tcttgtcgat cttggcgcca
     2101 cgggtccgat agtaccgatt gccgaactcg tcttccccga cgaattcgcc gtacatccag
     2161 gtccagaatt gcgtcccgaa agtctgcgaa ttccaccagg tgaacaattt aagcaggaaa
     2221 cttttcattg gcgccgcgca ttggaacctg ttgcaacgcg ggccttttgc caccggcccg
     2281 taaccttgtc cagcggggca cgctgggccc cggtatggta ttcgcttccc tgatatggat
     2341 gatatgaaac gcgcatgaat tcgctcggag ccgcgctgac ccaggtcaag gatctgctcg
     2401 attggctgcc ggatccggtg gtcgcactgc ttatcctcgc gatcgcggtg ctcattgcgc
     2461 tcgtgctcca ccgctgggcg cgcaagctgg tgcgccgggc cctcgccggg cgctaccctt
     2521 atgtcttctc ggtcttcacc cagacacgca accttacacg tctcgcgctg ctgatccttg
     2581 cgatgatcgt ggcaattccg gttgccccgt tgccgccgga gacggcggta cttctcgcgc
     2641 gcgcgatggg cgtcgcggtg atcggcctca tcggctgggc ggcgataatt gcgctgcata
     2701 ttgccgccga tctttacctg cggcgcttcc gccttgacat cgacgacaac ctgcttgccc
     2761 gcaagcacaa cacgcaggtg cgggtgctct cgcgcaccat cgatgtgctg ctgatcatgc
     2821 tcacgctcgg cgccgcactg atgactttcc cggccgtgcg gcaatacggt gtcagcctct
     2881 ttgcctccgc cggcgtcgcg ggcattgtcg caggtctcgc cgcaaggccc gtgctcagca
     2941 atctgatggc gggcgtgcaa ctcgccatga cgcaaccgat ccggctctat gacgcggtca
     3001 ttgtcgagaa cgagtacggc accgtcgagg agatcacatc cacttacgtg gtcgtgaagc
     3061 tctgggacct gcggcgcatg attgtgcctc tcacctattt catcgagaag ccgttccaga
     3121 actggacgcg cgaaagctcc tcgctcatcg gcaacgtgat gatctacctg gactatcgcg
     3181 cgccgatcga cctgatccgc gagaagttcg cggcgatcct caaggaatcg caccgatggg
     3241 atggccaagt atccgccgtg caggtcaccg atttcaagga aggcacgatg gaggtgcgtc
     3301 tgctgatgag cgcccgctcg tccggccagg ccttcgatct gcgctgcgag gtgcgagaaa
     3361 agctgattga gtttcttcag cgcgagcatc cggaagcgct gccgcacacg cggcaaatcg
     3421 ccttcagagg cgactccggc gccgcagccg gctccgagaa cgagggcaga agagctgccg
     3481 caaggcgtta acttgccgcc gacccctgcc agacacggct ttcacgggtt caatgctctc
     3541 ggcggcgaga tcgtttcgcg gctgggttcc gtgccgcacc aattcacagc gctgagtaag
     3601 ccggctgaag ggcagcgcat ccgcggcctg tggggtaacg cactgaattg gttccgagaa
     3661 tcagcgactc caaagccgga tagacaccgc ttttccccgc tctgtccccc gcctcgccct
     3721 cgactgcttt tgcgccccgg cgcatgcaag tcccattttt ccgtcatttt tctccgatgg
     3781 ccggaggcat aaaaacctaa gttcgccaat cgagaaaaat gcttttcgga cgtccgcgat
     3841 cgcccaatac tgccttcgtt ctcgcggtcc gtttgtcccc aaagttcact gttggacaca
     3901 agatttagtg tttgattcag atttaccgac tatctcttgt tgacgcgtac ggcgagtcca
     3961 catagctttt ctttgttcgg ttggcgttga gtttcaggtt ccctccggac cctcagaacg
     4021 gcttttcagt cgatgagctc aacccacgcc ggaacatggc gtcttgggac agggatttgc
     4081 tgtgtgccgg ggcaaaaacc ccgggtcgaa ccggtgccgc ttcgaagcat cggaccctag
     4141 atgagtggga tgggtagcgt cgaacgagtt cgagcaaggg cggcggacga ggacaagtcc
     4201 gccgaagaca aaggggcggt ggtcaatcgc cctgcaattt ggggctagaa aaatatgcga
     4261 attgagcggc gctataccaa ggatgaacag tcggcctatg cggacattgc cttccgtttc
     4321 accaccagcg agatccgcaa cccggatggc tcggtggttt tcaaggccga caatgtcgaa
     4381 gtgccggagt tctggtctca ggttgcctcg gacgtcctgg cgcagaaata tttccgcaag
     4441 gccggtgtcc ctgcccgcct gaaaaagatc gaagaaaatt ccgtcccctc attcctctgg
     4501 cgctcggtcg ccgacgaaga tgccctcgcc gcgctgcccg agaaggaacg ttacatcggc
     4561 gagacctccg caaagcaggt attcgaccgg ctcgccggca cctggaccta ctggggctgg
     4621 aagggcggct atttcgattc cgaaagcgac gcgcaggcct tcttcgacga gctgcgctac
     4681 atgctggccg cgcagatgtg cgcgccgaat tcgccgcagt ggttcaatac cggtctgcac
     4741 tgggcctacg gcatcgatgg cccgagccag ggccactatt acgtcgatcc gttcaccggc
     4801 aagctgacca aatcgaaatc ggcctacgag cacccgcagc cgcacgcctg tttcatccag
     4861 tcgatcagtg acgatctcgt caacgaaggc gggatcatgg atctgtgggt gcgcgaggcg
     4921 cggctgttca aatacggctc cggcaccggc tccaatttct cgctgctgcg cggcgaaggt
     4981 gagcgtctct cgggaggcgg caagtcatcc gggctgatgt cgttcctgaa aatcggcgac
     5041 cgcgccgccg gcgcgatcaa gtctggcgga accacgcggc gcgccgcgaa aatggtgatt
     5101 gtcgacgtcg atcaccctga catcgagcaa tacatcgact ggaaggttct cgaggagcag
     5161 aaggtggcgg cactcgtcac cggctccaag atcaaccaga agcacctcaa ggcggtcctc
     5221 aaggcctgcg tgaattgcga gggctcgggc gacgactgct tctcgccgga gaagaacccc
     5281 gcgctcaagc gtgagatcaa gctcgcgcgc cgcgcgatgg tgcccgacaa ctacatcaag
     5341 cgcgtgatcc agttctccaa gcagggctat aaggacatcc acttcccgac ctacgacacc
     5401 gactgggact ccgacgctta cctcactgtc tcgggacaga actcgaacaa ttcggtgcgc
     5461 gtcaccgacc acttcctcaa ggcggtcgaa gccgacggca aatgggatct caagttccgc
     5521 aagaacggca aggtcgcaca gactctcgat gcgcgcgcgc tgtgggagaa gatcggctac
     5581 gccgcctggg cctcggccga tcctggcctg caatatcaca cgacggtcaa cgactggcac
     5641 acctgcccgg cctcgggcga aatccgcgcc agcaatccat gttcggaata catgttcctc
     5701 gacgacaccg cctgcaatct ggcgtcgttg aacctgctca ctttccgcga cgagaagacc
     5761 ggcgaatttc aggtcgagag ctacgagcac ggagtccgcc tgtggaccgt cgttctcgaa
     5821 atctcggtga tgatggcgca gttcccgtca aaacagattg ccgaactgtc ctacgaattc
     5881 cgcacgctcg gcctcggcta cgccaatatc ggcggcctgc tgatgacctc cggcatcccg
     5941 tatgattccg attccggccg cgccatctgc ggcacgctga gcgccatcat gaccggcgtc
     6001 tcctacgcga cctcggctga aatggccgag aagctcgggc ccttccccgg ctacaagaag
     6061 aaccgcgagc acatgctgcg cgtgatgcgc aaccaccgcc gcgccgccta cggcgagcgc
     6121 actggctatg agaaggtcgc aacgccgccc gtgccgctgg atcattccgc ctgccctgac
     6181 cagacgctga ttgcgcatgc caagacggcc tgggaccgcg cgctgacgct cggcgagaag
     6241 catggctacc gcaatgcgca ggcgacggtg atcgcgccca ccggcacgat tggcctggtg
     6301 atggactgcg acaccaccgg catcgagccg gatttcgcgc tggtgaagtt caagaagctc
     6361 gccggcggcg gctacttcaa gatcatcaac cgcgcggtgc ccgaagcgct gcgcacgctt
     6421 ggctacagcg aagccgacat cgccgagatc gaggcttatg cggtcggcca cggcacactg
     6481 tcgcaggcgc cggcgatcaa tcacacaacg ctcaaggcca agggcttcac cgatgaggcg
     6541 atcgccagga tcgagaaagc gctgccgacg gccttcgata tcaagttcgc cttcaacaaa
     6601 tggacgctgg gcgaggattt ctgccgcgac aagctcggcg ttgacgccgg agatctcgcc
     6661 tcgccggcct tcgatctcct cgccgcgctc ggcttcggca agcgagagat cgaggccgcc
     6721 aatatccacg tctgcggcgc aatgacggtc gaaggtgctc cgcacctgaa ggccgagcac
     6781 tatccggtgt tcgactgcgc caatccgtgc ggccgcaccg gcaagcgtta tctctcggtc
     6841 gagagccaca tccgcatgat ggcggcggcg cagcccttcg tctcgggcgc catctccaag
     6901 accatcaata tgccgaacga ggcaacggtc gaggattgca aggcggccta tctgctctcg
     6961 tggaagctgg cgttgaaggc caacgcactc taccgcgacg gctcgaaact gtcccagccg
     7021 ctcaacgcgc agctcatcgc cgacgacgat gatgaggatg atgcggtcga gatgtttctc
     7081 gaccgtccgg ccgcagcccg cgcgacggga ctcgccgaga aggtggtcga gaagatcgtc
     7141 gagcgcatca ccgtgctgcg tgagcgcgag cgcatgcccg accgccgcaa gggctatacc
     7201 cagaaggcgg tcgtcggcgg ccacaaggtc tatctccgca ccggcgaata tgacgacggc
     7261 cggctcggcg agatcttcat cgacatgcac aaggaagg
//
LOCUS       DASSRL010000326         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_428498, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000326 DASSRL010000000
VERSION     DASSRL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428498"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..582)
                     /gene="erpA"
                     /locus_tag="VFL49_06690"
     CDS             complement(253..582)
                     /gene="erpA"
                     /locus_tag="VFL49_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET7911698.1"
                     /translation="MSTSVTVSERAAKRIGEILKGEPAGTMLRVSVEGGGCSGFQYKF
                     DMERAKATDDLVISRDNAVILIDPVSVNYMAGSEIDFVDDLIGASFKVNNPQAKASCG
                     CGTSFAL"
     gene            666..1871
                     /locus_tag="VFL49_06695"
     CDS             666..1871
                     /locus_tag="VFL49_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET7911699.1"
                     /translation="MAVETGCERAVYAADPAQSRGRRHAETTSPTRNAFRRDCDRIIH
                     SAAFRRLAHKTQVFVYHEGDHYRTRLTHTLEVAQIARSLARALGLDEDLAEALALAHD
                     LGHPPFGHAGERALDECLSALGGFDHNAQALRIVTDLERRYAAFDGLNLSWETLEGLV
                     KHNGPLFNRDGAAVGRYARHGAPAAIVAYSKIQDLELWSYASAEAQAAALSDDIAYDA
                     HDIDDGLRADLFTLEDISAVPFVGAMLADIDREYPRLETARRVHELVRRIITRMIEDV
                     IAESGRRVAMLKPRSAVDVREAPRPIVGFSPAMDKADRDIKGFLYPRMYRHDRVMRVM
                     ADAEGVVRDLFAHYTATSADLPDEWREGVGTNDADSARRVADYIAGMTDRYALIEHAK
                     YFKGTPELR"
     gene            1877..>2689
                     /locus_tag="VFL49_06700"
     CDS             1877..>2689
                     /locus_tag="VFL49_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7911700.1"
                     /translation="MDENREVDLLVAGAGPAGLTAALVASLEGLDVLLCEKSDQVGGT
                     GSTSAGTLWIPGNSQSRSAGFADSAEQADRYLDTLIGEKTNRAIRNAFLQSGPKVIDY
                     LSARTDVQFLPCGKHPDYRSNMPGAAISGRAIVPVPFDGRLLGADFGRIRPPVPEFMV
                     FGGMMVGKADIPRLIGRFRSISNFVYSAKLFARYLADRLRYRRGTRIMMGNALIGRLF
                     YSLRKRNVHILYGARIVDLIGDRRGITGARLAVGGDQITIKVRKGVVLATGGY"
BASE COUNT          502 a          814 c          853 g          520 t
ORIGIN      
        1 gctcaagcgc cgtctcgagc cgcagcttgc cgaagttgag gaagctccgc ccgcagtcgc
       61 ggcggaataa acttcgaaat tgtcatggcc gggcaaccgg gtccggcaaa ggccggcccg
      121 gtcgtaaact tgtcccggcc atccacgact tctctgccaa agacgtggat gcccggcaca
      181 aggcctgtgc tcgggccggc cgaaggccgg acccgagtgc cgggcatgac gaaggcaaga
      241 ccgtgcgagt ggttaaagcg cgaaactggt gccgcaaccg caggaagctt tggcctgcgg
      301 gttattcacc ttgaacgacg cgccgatcag atcgtcgacg aagtcgatct ccgagccggc
      361 catgtaattc accgacacgg ggtcgatcag gatcacggcg ttgtcgcggc tgatgacaag
      421 atcgtcggtc gccttggcgc gctccatgtc gaatttgtac tgaaagccgg agcagccgcc
      481 gccttcgacg ctgacgcgca gcatggttcc ggccggctcg cccttgagga tttcgccgat
      541 ccgtttcgcc gcccgttcgc tgaccgtgac gcttgtgctc atcgcctgtg ccttttgacg
      601 gccatcctat tggcagattg cccaacggat agttaagtgc gcgggccagc gaggtcaatg
      661 tatccatggc ggtcgaaacc gggtgtgaaa gggcggttta cgcggccgat ccggcgcaaa
      721 gccggggcag gcgacatgcg gagacgacaa gcccgacgcg caatgctttc aggcgggact
      781 gtgatcggat catccattcc gccgctttca ggcggctcgc ccacaaaacg caggtcttcg
      841 tttatcatga aggcgatcat tatcgcacgc ggctgacgca cacgctggaa gtcgcccaga
      901 ttgcccggtc gcttgcgcgc gcgcttgggc tcgatgaaga cctcgccgaa gcattggcgc
      961 ttgcgcacga tcttggtcat ccgccgttcg gccatgcggg cgagcgcgcc ttggacgaat
     1021 gcctttccgc cttgggaggc ttcgatcaca acgcgcaggc cctgcgcatt gtgacggatc
     1081 tcgagcgccg ttacgcagcg tttgacggcc tgaacctgag ctgggagacg ctcgagggtc
     1141 tggtcaagca caacgggccg ctcttcaatc gcgacggcgc tgcagtcgga cgctatgcga
     1201 ggcacggggc gcccgccgcg atcgttgcct actcgaaaat tcaggatctc gagttgtgga
     1261 gttacgccag cgcggaagcg caggccgcgg ccttgtcgga cgacatcgcc tacgatgccc
     1321 acgatatcga cgacggcttg cgtgccgacc tgttcacact tgaagatatt tcggcggtac
     1381 cgtttgtggg ggcgatgctt gccgacatcg atcgagagta tcctcgcctc gagaccgcac
     1441 ggcgcgtgca cgaactcgtt cgtcgcatca ttacacgcat gatcgaggat gtgattgccg
     1501 aaagcgggcg tcgcgtcgcg atgctcaagc cgcggtcggc cgtggacgtg cgcgaggcac
     1561 cccggccaat cgtgggattc tcacccgcca tggacaaggc cgaccgggac atcaagggct
     1621 ttctctatcc gcgcatgtat cgccatgacc gtgtgatgcg cgtcatggct gacgccgaag
     1681 gcgtcgtgcg cgacctgttc gcgcattaca cggcaacgtc ggcggatttg ccggacgaat
     1741 ggcgcgaagg cgtagggacg aacgatgccg acagtgcgcg ccgcgttgcg gattacattg
     1801 cagggatgac cgatcgatac gcactgatcg agcatgcaaa atatttcaag ggaacgcccg
     1861 agcttcggtg atgagcgtgg atgagaaccg cgaggtcgac ctcttggttg ccggtgctgg
     1921 tccggctggc ctgactgcgg cgctggtcgc ttcgctcgaa gggctcgacg tgcttttgtg
     1981 cgagaaatcg gatcaggtcg gcggcaccgg ctcgacgtcg gccggcacgc tctggattcc
     2041 cggcaacagc caaagccggt ccgccggctt cgccgatagt gccgagcagg ccgaccgcta
     2101 cctcgataca ctgatcgggg agaaaaccaa ccgcgcgatc aggaatgcat ttcttcagag
     2161 cgggccaaag gtgatcgact acctgagcgc acgcaccgac gtgcagtttc ttccatgcgg
     2221 caaacatccg gactatcgca gcaacatgcc cggtgcggcc atttccggcc gcgccattgt
     2281 gcccgtaccg ttcgacggcc gtctgcttgg cgcagatttc ggacgcattc gaccgccggt
     2341 gcccgagttc atggtcttcg gcggcatgat ggttggcaag gcggacattc cacgactgat
     2401 cggccgcttc cgttcgattt ccaattttgt ctattcggca aaattatttg cacgctacct
     2461 ggccgaccgc ctgagatatc ggcgtggcac ccggatcatg atgggcaacg cgctcatcgg
     2521 tcggctgttc tacagcctgc gcaaacgcaa tgtgcacatc ttgtacggag ccaggatcgt
     2581 cgatctcatc ggtgaccggc gcggcattac aggagctcgt ttagctgttg gcggcgacca
     2641 aataacgatc aaagtgcgca agggtgtcgt gcttgcgacc ggcggctat
//
LOCUS       DASSRL010000327         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_218907, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000327 DASSRL010000000
VERSION     DASSRL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218907"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFL49_06705"
     CDS             <1..412
                     /locus_tag="VFL49_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HET7911701.1"
                     /translation="ATAPSDVLVDLIFALKAGYRQNAAFVMNRKTQSLIRKFKDTTNN
                     YIWQPPASPSGRASLMNFPVHDVEDMPDIAANSLSVAFGDFMRGYLIVDRAGVTLLRD
                     PYTAKPYILFYTTKRVGGGVQDYDAIKLLKFAVS"
     gene            699..1337
                     /locus_tag="VFL49_06710"
     CDS             699..1337
                     /locus_tag="VFL49_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911702.1"
                     /translation="MSTSSPVRTRQPRRPSRLPQARSNPSPRRGPVVVRSILLVVAAV
                     SVIATSNYLAYLIGRKQVTYEELRRQVDRVVQLIDEKRVEDDLAATTLKQRQERLEQN
                     AQGLSGLTTSTINGKTTVVAPEPAPAEGPTPAAPIEAKPVARPDSEASLQPPESSPNA
                     TTKHRDTHGVAHQRGPGRAKAPKRAPTEALAEQPGQSTTATAPTPDAGLARQ"
     gene            complement(1371..2765)
                     /locus_tag="VFL49_06715"
     CDS             complement(1371..2765)
                     /locus_tag="VFL49_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7911703.1"
                     /translation="MSETQALFDTLRNSADRAVVAAIESAARDGTDRQLCRINVLTFA
                     ARHGLDEEKSISGFLHAARLGLFELSWNVLCPGCGGVLEATQTLRSVHKEAYDCALCA
                     AGYEPTLDEMVEVAFTVSPRLRRIAAHDPHSLPVWEYFRQIFWGSGIDLPETGFEELL
                     EKITIEAIELPPGEKALISLQLPAEFVIVFEPVTHAAHFIDVKGEPTRERQSLTMIFN
                     RIHAPTGTTTMRPGPLRLALENRSDVRTLPSVWIAGDELHHLLGKRRPFLTAKRLLSN
                     QTFRDIYRTDTLDVDQGLKVTSLTFLFTDLKGSTALYDRVGDIVAYDLVREHFRILQD
                     IVSSEGGAVVKTIGDAVMATFPTPDRAVSAALRMRDSVQKLKNDLLIKIGIHEGPCLA
                     VTLNDRLDYFGQTVNIAARVQSLADSRAIFATKSVVENSQVSKILEAGEFKLTEQSAA
                     LRGVADKMAVFQIP"
BASE COUNT          579 a          882 c          894 g          558 t
ORIGIN      
        1 cgcgacggcg ccatcggatg tgctggtcga tctcatcttc gcgctcaagg cgggttaccg
       61 ccagaatgcg gcatttgtga tgaaccgcaa gacgcagtcg ctgatccgca agttcaagga
      121 cacgaccaac aactatatct ggcagccgcc ggcttcgccg tcgggacgcg ccagcctgat
      181 gaatttcccg gtgcatgatg tcgaagacat gcctgacatt gcggccaatt cgttgtccgt
      241 tgcatttggc gacttcatgc ggggctacct catcgtggac cgcgcgggcg tcaccttgct
      301 tcgcgaccct tacacggcaa agccctacat acttttttac acgaccaagc gcgtcggcgg
      361 cggcgtgcag gattatgacg cgatcaagct gctgaagttt gcggtgagct gatccgacac
      421 ttttttacca ctgactcaga cagggcgccc aaaaggcggc ctgtctcttt cagcaacaga
      481 gctctctcat gagagcttgt agaccgcacg tcggctgctt tccgttgctt gaagatgaaa
      541 tatgccattt cagtttcgac ggactcccat caggccgctc gcgggatcgc ctcgatgcga
      601 gtagcagaag gtattcgcgt gagcaggacg gcaatctttg ctttgactca tcacggcgaa
      661 tctggctcgg catatgtcca accggtacgg agcgctgcat gtcgacctct tccccggtac
      721 gcactcgcca accacgccgt ccgtcacgcc tgccgcaggc gcggagcaat ccgtccccgc
      781 ggcgtgggcc cgtcgttgtc aggtcaattc tgctggtcgt tgcggcggta tcggtgatcg
      841 caaccagcaa ctatctggcg tacctgatcg gacgcaagca ggtcacctac gaggaactgc
      901 gcaggcaggt cgaccgcgtc gttcagttga tcgacgagaa gcgcgtcgaa gacgacctcg
      961 cagccacaac gttgaagcag cggcaggaga ggttggagca gaatgcgcag ggcctcagcg
     1021 gcctgacgac gagcacgatc aacgggaaga caacggtcgt tgcgcccgag ccggcgcctg
     1081 ccgaaggacc gacacccgcg gcgccgattg aggccaaacc cgttgcgcgg ccggacagtg
     1141 aagcgagctt gcagccgcct gaatcgtcac cgaatgccac aacgaaacat cgcgacacgc
     1201 atggcgtggc ccatcaacgc ggtccaggcc gggcgaaggc accaaagcgg gctcccacgg
     1261 aggcgcttgc cgagcagccg ggtcaatcaa caacggcgac agcgcccaca ccggacgcgg
     1321 gcctcgcccg tcaataattt ggaggcgagt cgcgtcacgc tgattgccgt tcaaggaatt
     1381 tgaaagaccg ccattttgtc ggctacgccg cgcagggccg cgctttgctc ggtcagcttg
     1441 aactcgccgg cctcgaggat cttcgatacc tgcgaatttt ccaccaccga ctttgtcgcg
     1501 aagatcgcgc gtgaatcggc aaggctctgc acgcgggcgg cgatattgac ggtctggccg
     1561 aagtaatcga gccgatcgtt aagggtgacc gcgaggcatg gtccctcgtg aattcctatc
     1621 ttgatcagca ggtcgttctt cagtttttgc acggagtcgc gcatgcgaag cgcggcgctc
     1681 acggcgcgat caggcgtcgg aaaagttgcc atcacggcgt cgccgatcgt tttgacgacg
     1741 gcgcctccct ccgaggaaac gatgtcctgt agaattctga aatgctcgcg caccagatcg
     1801 taggcgacga tgtcgccgac gcggtcgtaa agcgccgtcg agcccttgag atcggtaaac
     1861 aggaaagtga gacttgtgac tttcagtccc tggtccacat cgagcgtatc agttcggtag
     1921 atgtcacgaa aagtctggtt gctcagcagc cgctttgcgg tcaggaacgg tcgccgcttg
     1981 ccgagcaggt gatgcagctc gtcgccggcg atccacaccg acggcagcgt gcgcacgtcg
     2041 ctgcggttct cgagtgcaag ccgcagcggg ccgggccgca tcgtcgtggt gccggtcggg
     2101 gcgtgaatcc tgttgaagat catcgtcagg ctttggcgct cgcgggtcgg ctcccctttc
     2161 acgtcgatga aatgggccgc gtgcgtgacc ggttcgaaga cgatcacgaa ttccgccggc
     2221 agctgcagtg atatcagggc cttctcgccg ggcgggagct cgatggcctc gatggtgatt
     2281 ttttcgagaa gctcctcaaa tcccgtttcc ggcaggtcaa tccccgagcc ccagaaaatc
     2341 tgccggaaat attcccatac cggaagcgag tgcgggtcat gtgccgcgat acggcgcagg
     2401 cggggactga cggtaaaggc gacttcgacc atctcgtcga gcgtcggctc atagccggcc
     2461 gcgcacagcg cgcagtcata cgcctccttg tgcacggacc tcagcgtttg cgtggcctca
     2521 agcaccccgc cgcaaccggg gcagagaaca ttccagctca actcgaacag tcccagccgg
     2581 gcagcatgca ggaacccact gatcgatttt tcttcgtcca gcccgtgcct ggcggcgaag
     2641 gtcaggacat tgatacggca caattgccgg tcggttccgt cgcgcgccgc cgattcaata
     2701 gcagcgacga cggcgcggtc agccgaattg cgcagcgtgt cgaaaagcgc ctgagtttcg
     2761 ctcatggtcg gcaggttacc ccaaaatcgg taaagttgga ggcgcagacc gccgtgcttg
     2821 ccgactcgac ggcgaactga gcgaggaccg gtcggtccgg acccagtacg atttgaataa
     2881 gagaagggct ttggtgttag tgccgtatgc gat
//
LOCUS       DASSRL010000328         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1128784, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000328 DASSRL010000000
VERSION     DASSRL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128784"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFL49_06720"
     CDS             complement(<1..794)
                     /locus_tag="VFL49_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911704.1"
                     /translation="MTIEMRLPWTRLPATQAQSPRASGWLSTIRGRLYVAFGFAAALT
                     IIGSSIAFYEFTIIGSTTNEIVSRSLPTTAVSLRLAEQASSLVSSGPRLMAAATDKVR
                     AEILEDINKQEKNLEAGIAQLKTLGVGNADEIEVSRKALAQRLQTLNQAVSNRISMSS
                     ERSYLASSVRSAHEALLIGLAPAIDDANFDLMMNARQEGAAAIATRLEYLRRLLETES
                     ESNLLAGLLTEASLVSDTTRLEPLRDLIASAQRKITKNLNAMPDRA"
     gene            complement(791..2014)
                     /locus_tag="VFL49_06725"
     CDS             complement(791..2014)
                     /locus_tag="VFL49_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911705.1"
                     /translation="MRAPSYLTRNRWWDLSVRINRGWLCIFGAALICSLTVHARAEDG
                     VSKDSILFGQVAALNGPAQALGQGMRDGILAAFEDVNRSGGINGRKLELKSIDDGYEP
                     EKTIEATNRAIREDKVFALVGAVGTPTSKAGQPIATAAKVPFIGPFTGAEFLRNPFNR
                     YVVNIRSSYFQETEAWIEHLTNDLGVTRIAILYQDDAFGLAGLAGVKTAMEKRNMSLV
                     AEGTFKRNTTAIKSALLEIMKGQPQAIVTVGPYKPVAAFIKLARQLKVDATFVAISFV
                     GSDSLAQELGGEGAGVVVSQIVPFPGDRSLPVVASYQSALAALNEKSKPGFVSLEGYL
                     VGRLVVEALKRVPGEPTRDAFLDAIEREPFDLGGMKLTFSATQNQGSNQVFFTVLQSD
                     GLLRPVTKLVKTVAR"
     gene            complement(2087..2329)
                     /locus_tag="VFL49_06730"
     CDS             complement(2087..2329)
                     /locus_tag="VFL49_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911706.1"
                     /translation="MTISTAISIAMPESRRCKERALECRTVASRLRVQNARDQMLKAA
                     ADYERMARDAEQREIAVGLSHLRALAVRQRHGRQAA"
     gene            complement(2416..2586)
                     /locus_tag="VFL49_06735"
     CDS             complement(2416..2586)
                     /locus_tag="VFL49_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911707.1"
                     /translation="MPPFSAVDYRRYAAECIRLAQQVADPGEKARLLDMAETFRELAD
                     KSEAKQSGPAGD"
     gene            2723..2881
                     /locus_tag="VFL49_06740"
     CDS             2723..2881
                     /locus_tag="VFL49_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911708.1"
                     /translation="MLESEKFRQYAADCIRIARQMSGEDRQILLDIAAAWEMRAQDAE
                     KKEKGSRS"
     gene            complement(2944..>3508)
                     /locus_tag="VFL49_06745"
     CDS             complement(2944..>3508)
                     /locus_tag="VFL49_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911709.1"
                     /translation="IQQKPAPQTQADSAAATAASGAAPEHVIDSPAAGPARPAPSAGS
                     NAAPVSRAREPQRRQQMQVRPEPPAPAIARVDHTFALLMVMFAALAIAGPALHFVERR
                     RRREAANFRPPPWARVVALNAPKPRIRVMPRQKIANASRVPVPIRPPDQTERLAHALQ
                     QLVDRLQTSERPEPKAIRIRPRERASM"
BASE COUNT          655 a         1033 c         1090 g          730 t
ORIGIN      
        1 tgcgcgcgat caggcattgc gttcaggttc ttggtaatct ttctctgcgc ggatgcgata
       61 aggtcgcgca acggctccag acgcgttgta tcgcttacga gcgaagcttc tgtgagcaat
      121 ccggccagca ggttcgattc cgattccgtt tccaaaagcc ggcgaaggta ttcaagcctt
      181 gtcgcgatcg ccgcggcgcc ttcctgtctg gcattcatca tgagatcgaa attcgcatca
      241 tcaatcgccg gcgcgaggcc tatgaggagc gcctcgtgcg cggagcggac cgaggacgcc
      301 agataggacc gctcgctcga cattgaaatg cggttcgaaa ccgcttggtt gagtgtttgc
      361 agccgttgtg ccaacgcctt gcggctcacc tctatttcat cggcattgcc gacgccgagc
      421 gtcttcagct gtgcaatccc ggcctccagg tttttttcct gcttgttgat atcttccagg
      481 atttcagcgc ggactttgtc tgtcgcggcc gccatcagcc ggggaccgga ggagacaaga
      541 ctgcttgctt gttcggcaag gcggagtgaa acggccgtcg tcggaaggct gcgggagacg
      601 atttcgtttg ttgtggatcc gatgatggtg aattcataga acgcgataga ggagccgatg
      661 atggtcagcg ccgcggcgaa gccgaaggcg acgtacagcc ggccacgaat ggtcgagagc
      721 cagccagaag cacgaggact ttgcgcttgt gtcgccggta gtctggtcca gggcagacgc
      781 atctcgatcg tcaccttgcc acagtcttta cgagctttgt caccggccgg agaagtccgt
      841 ccgactgaag cacggtgaaa aagacctgat ttgatccctg attttgcgtt gcgctgaacg
      901 tgagtttcat gccgccgaga tcgaacggtt cgcgctcgat ggcgtcaaga aacgcgtcac
      961 gcgtgggctc gcccggtact cgtttaagcg cttcgaccac cagccggccg acgaggtagc
     1021 cttcgagcga tacaaatcca ggcttcgatt tttcgttcag cgccgcgagc gcggactggt
     1081 atgacgccac gaccggcaat gacctgtcgc cggggaaggg tacgatctgg cttacgacga
     1141 cgcccgctcc ttcgccaccg agttcctggg cgagcgaatc cgaccccacg aatgaaatcg
     1201 cgacgaaggt cgcatcgacc ttgagttggc gcgcaagctt gatgaacgct gcgaccggtt
     1261 tgtacgggcc caccgtgacg atcgcctgcg gctgaccctt catgatttcg agcaacgccg
     1321 acttgatggc agtggtgttg cgcttgaacg tgccttccgc gaccagcgac atgttgcgct
     1381 tttccatcgc ggtcttcacg ccggcgaggc cggccaggcc gaaagcgtcg tcctggtaca
     1441 gaatggcgat tctggtaacg ccaagatcat tggtcagatg ctcgatccag gcttcggtct
     1501 cctgaaagta cgagctgcgt atattgacca cgtagcgatt aaacggattg cggagaaatt
     1561 ccgctccggt gaaaggtccg atgaaaggca ccttggcggc agtcgcgatc ggttggccgg
     1621 ccttcgatgt cggcgttccc actgcgccga caagtgcaaa cactttgtct tccctgattg
     1681 ccctgtttgt cgcttcgatg gtcttttccg gctcgtagcc gtcgtcaatc gacttgagtt
     1741 cgagcttgcg gccgttgatg ccgccggagc gattcacgtc ctcaaatgcc gcaagaatgc
     1801 cgtcgcgcat tccctggccc aaagcctgcg ccggaccgtt gagggcggcg acttgtccga
     1861 aaagaatgct gtctttggat acgccgtctt ccgcacgcgc atgtacggtc aaagaacaaa
     1921 tgagtgctgc tccgaaaatg cagagccagc cacgattgat gcgcacggac agatcccacc
     1981 agcggttgcg cgtaaggtag gatggtgcgc gcacatggtt tacgaatgat taagaaccgg
     2041 tcgaaggccg ccttctcgca tttgttcgag gacggattgc cactgatcag gcggcctgcc
     2101 ggccgtgacg ctgcctgacg gcaagtgccc tcaagtgcga caggcccacg gcgatttccc
     2161 gttgctcggc gtcgcgtgcc atgcgctcgt agtcggcagc ggccttcaac atttgatcgc
     2221 gcgcattttg cacgcgcagc cggcttgcca ccgtgcggca ttcaagcgcc cgttctttgc
     2281 acctccggct ttcgggcatg gcaattgaaa tggcagttga aattgtcatt gcaggcggcg
     2341 ccttggatca ggactcctga gaaacacaca gcgcgtttgc cggttccgcg gcatgagaaa
     2401 aatgaaccgc cggggtcagt ccccggcagg gcctgattgc ttggcctcgc tcttgtcggc
     2461 gagttcccgg aaagtttcgg ccatgtcgag cagccgcgct ttctcgccgg gatcggcgac
     2521 ctgctgcgcc aaccttatgc attccgcggc gtatcgacga taatcgacag cggaaaatgg
     2581 cggcacccaa cgctcccctg cacgcagcgg tagaagtcgc gtccgggtcc attggttccc
     2641 tgccaccttc cttcacaata ttgttggaga acaaagttcc aaaaatcgac gttgcctcct
     2701 tgcggggatt gaggaggcga ggttgctgga gtcggaaaaa ttcaggcaat acgcggcgga
     2761 ttgcatccgc attgcgcgcc agatgagcgg ggaggaccgg caaatcctgc tggacatcgc
     2821 cgccgcctgg gaaatgcgtg cgcaggacgc ggaaaaaaag gaaaagggtt cccgcagcta
     2881 gaggatttga ggcgcaacac aatcgggccg atgtctcatc tctcgctatt ccggacaccg
     2941 ctttcacatt gacgcgcgct cgcgcggtct gatgcggatt gcctttggct ccggccgctc
     3001 gcttgtctgc agacggtcca ccagttgctg cagcgcatgc gccagtcttt ccgtctgatc
     3061 gggcggcctg atcggcacag gcacacggga cgcattcgcg attttctggc gcggcatcac
     3121 acggatgcgc ggtttcggcg cgttcaatgc gaccacgcgt gcccatggag gcggccggaa
     3181 attggcggcc tcgcgtcggc ggcggcgttc aacaaaatgc agggccgggc cggcaatcgc
     3241 cagcgccgca aacataacca tcagcaacgc gaatgtgtga tcgacgcggg caattgcagg
     3301 ggccgggggt tcggggcgga cctgcatctg ctgacgacgt tgcggctctc ttgcgcgcga
     3361 caccggcgcc gcgttgctgc cggcgctcgg agcgggacgg gcgggaccag cggccggcga
     3421 atcgatgacg tgctcgggcg ccgccccgga cgcggcggtc gctgcagcac tgtcagcttg
     3481 cgtctgcggc gccggctttt gctgtatc
//
LOCUS       DASSRL010000329         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1128792, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000329 DASSRL010000000
VERSION     DASSRL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128792"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFL49_06750"
     CDS             <1..632
                     /locus_tag="VFL49_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7911710.1"
                     /translation="IASISEIGRQISQAAEVASHSVAEAQTTKEHMARLTDTVQEIGA
                     VVNLIRNIAGQTNLLALNATIEAARAGEAGRGFAVVASEVKSLAVQTAKATEQIASQI
                     DAVQSSTRVAVDAIRRNTDRMREIDGYTSAVALSLQQQDNATDEISHNVASAANGAKG
                     MVSVLEEVTRAVGDTRSAAGKVLQASETVETAATSLQRGIEGFLSRVAV"
     gene            complement(636..1493)
                     /locus_tag="VFL49_06755"
     CDS             complement(636..1493)
                     /locus_tag="VFL49_06755"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7911711.1"
                     /translation="MAKKKIIFQGEPGANSHLACREAYPDHEPVPCPTFEDCFAALVA
                     GDVDLGMIPIENSVAGRVADIHHLMPTAGLHIVAEWFLPIRNQLMAVKGASLKTIKTV
                     QSHVMALGQCRNVIRELKLKPIVAADTAGSAREVSETKDPTRAAIASRLAAEIYGLDI
                     LKENVEDESHSTTRFVVLSREKKWAKPGTGKVVTTFVFRVRNVPAALYKALGGFATNG
                     VNMTKLESYMVGGNFFATQFYADVEGHPDDRALVFALEELAFFSKELTVLGVYPTHPF
                     RETFKEEKD"
     gene            complement(1539..2267)
                     /locus_tag="VFL49_06760"
     CDS             complement(1539..2267)
                     /locus_tag="VFL49_06760"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET7911712.1"
                     /translation="MPDTLILIPARLTSTRLPGKPLADLHGAPMIVHVLRRAEAAGIG
                     DAIVATDSEAVAAAVEKGGGRAVMTRSDHESGSDRIYEALEAVDPERRVETVVNLQGD
                     LPTIGAADIRASLKPLADGNVDIATLASVITDPAERTNPNVVKVSGREVAPGHLQATA
                     FTRKDAAGPGPHYHHIGLYAYRRAALERFVKLPPSAGERREMLEQLRALDNGMRIDVT
                     VVKSVPLGVDTPDDLEKARKLLAG"
     gene            2422..>2973
                     /locus_tag="VFL49_06765"
     CDS             2422..>2973
                     /locus_tag="VFL49_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HET7911713.1"
                     /translation="MNSFELNKILGAVLGTCLIMLALNIGAGAIFAPEKPAKPGFNIA
                     VKEESGGEKAGAPKEPEQPIETLLAKASVEKGQTIAKQCAACHTFEKGGPNRVGPNLW
                     NIVGSDRGEGRGGFNFSAAMKAKGGKWTFDELNKFLANPRQYIPGTAMTFAGISKPEQ
                     RADVIDYLRTLADNPVPLPKAAEG"
BASE COUNT          593 a          898 c          926 g          556 t
ORIGIN      
        1 tgatcgcgtc aatcagtgaa atcggacggc agatcagcca ggccgccgag gtcgcgtccc
       61 attcggttgc cgaagcgcag acgaccaaag agcacatggc tcggctcacc gacaccgtcc
      121 aggagatagg cgcggtcgtc aacctgatcc gcaatattgc gggccagacg aatctgcttg
      181 cgctcaatgc cacgattgaa gccgcgcgtg cgggtgaggc cggccgcggg ttcgccgtgg
      241 ttgcttccga agtgaaatca cttgccgtgc agaccgccaa agcgaccgag caaattgcca
      301 gccagatcga tgcggtgcag agctcgacgc gagtggccgt ggatgcaatt cgccgcaata
      361 ccgaccggat gcgcgaaatc gacggctata cctcggctgt ggcgctgtcc ctgcaacagc
      421 aggacaacgc gaccgacgag atctcgcaca acgtggcgag cgctgcgaat ggagccaaag
      481 gcatggtctc ggtcctcgag gaagtcacgc gggcggtcgg ggacacccgc agcgcggccg
      541 gcaaggtgct gcaagcatcg gaaaccgtcg aaaccgcagc gacaagcctc caacgcggta
      601 ttgagggatt cctctcccgt gttgccgtgt gacgactagt ctttctcttc cttgaatgtc
      661 tcgcggaagg ggtgcgtcgg gtagacgccg agcaccgtca gttccttgga gaaaaacgcc
      721 agttcctcga gcgcgaagac cagcgcccga tcatcgggat gcccctcgac gtcggcatag
      781 aactgcgtag cgaaaaagtt tccgccgacc atataacttt ccagcttcgt catgttgacg
      841 ccgttggtcg cgaacccgcc gagcgccttg tagagcgctg cgggcacgtt gcgcaccctg
      901 aacacaaacg tcgtgacgac tttgccggtg cccggcttcg cccatttctt ctcgcgcgag
      961 agcacgacga agcgcgtggt tgagtgagac tcgtcctcga cattttcttt gagaatgtcg
     1021 agaccgtaga tctccgctgc cagccgcgag gctatcgcgg cgcgggttgg gtccttggtc
     1081 tccgagacct cgcgtgccga tcccgcggta tcggcggcga cgatcggctt caacttcagc
     1141 tcgcggatga cgttgcggca ttggccgagc gccatcacgt ggctctgcac agtctttatc
     1201 gtcttgaggc tcgcgccctt cacggccatc agttgattgc ggatgggcag aaaccattcg
     1261 gcgacgatgt gcagccccgc ggtcggcatc aggtgatgaa tatcggcgac gcggcctgct
     1321 acggaatttt cgatcggaat catgccgaga tcgacgtcgc ccgcaacaag cgctgcgaag
     1381 caatcctcga aggtggggca gggcacgggt tcatgatccg gataggcctc acgacaggca
     1441 agatgcgaat ttgcccccgg ttcgccctga aaaatgatct tctttttggc catatcccac
     1501 cttgtcgtgc gttccgtacg cagacattac cggttgagtc aaccggcgag cagcttgcgc
     1561 gccttttcca gatcgtcggg cgtatcaacg ccgagcggca cgcttttcac gaccgtcaca
     1621 tcgatgcgca tgccgttgtc caacgcgcgc aattgctcca gcatctcgcg ccgctcgccc
     1681 gcggacggcg ggagtttcac aaagcgctcg agcgccgcgc ggcggtaggc gtaaaggccg
     1741 atgtggtgat agtgcggccc gggaccggct gcatccttcc tggtgaaggc cgtggcctga
     1801 aggtgaccgg gcgcaacctc gcgaccggat actttgacga catttgggtt tgtccgctcg
     1861 gccggatcgg taatgacgga ggccagcgtt gcaatgtcga cattgccgtc agccagcggc
     1921 ttgagcgagg cgcgaatatc cgcagcgcca atagtcggca aatctccctg gaggttgaca
     1981 acggtttcaa cgcggcgttc ggggtcgact gcttcgagcg cttcatagat gcggtcggag
     2041 ccggactcgt gatcgctgcg cgtcatgacc gcacggcccc cgcccttttc cactgccgct
     2101 gcaacagcct ccgaatcagt tgcgacgatg gcgtcgccga ttccagcggc ttcggcgcgg
     2161 cgaagcacgt gcacgatcat cggcgcgccg tgcaggtccg ccaatggctt gccgggcaag
     2221 cgagtcgacg tcaggcgcgc gggaatgaga atgagcgtat cgggcatcgg gtgcgggggc
     2281 acatgtgacg aatgtcgaaa atgccgcagg cgcttatacg ggttgccaga ggccaggcaa
     2341 accggtatct gatcgccgac gccaggtccc tggctgtatc cgctccgcac cgttacgccc
     2401 cgcttcggga gccgaatttc gatgaattcc ttcgaactga acaaaatcct cggcgccgta
     2461 ctcggcacct gtctgatcat gcttgcgctc aatatcggcg ccggtgctat ttttgcgccc
     2521 gagaagccgg ccaagcctgg gttcaatatt gccgtcaagg aagagagcgg cggtgagaaa
     2581 gccggcgcac ccaaggaacc ggagcagccg atcgagaccc ttctcgcgaa ggcatcggtg
     2641 gaaaaaggtc agacgatcgc caagcaatgt gctgcctgcc acacgttcga gaagggcggc
     2701 cccaatcgcg tcggcccaaa tctctggaat attgtcggga gcgaccgtgg ggaaggccgc
     2761 ggtggtttca acttctcagc cgcaatgaag gccaaaggcg gaaaatggac ctttgacgag
     2821 ctcaacaagt ttctcgcaaa tccgcgccaa tatatcccgg gaacggcgat gacgttcgcc
     2881 ggaatttcaa agcccgagca gcgtgccgac gtgatcgatt acctgcgcac gctggcagat
     2941 aatccggttc cgttaccgaa ggctgcggaa ggt
//
LOCUS       DASSRL010000330         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_411479, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000330 DASSRL010000000
VERSION     DASSRL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411479"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFL49_06770"
     CDS             complement(<1..191)
                     /locus_tag="VFL49_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7911714.1"
                     /translation="MSQIEDIVSRLMALHPKRIDLSLGRVQRLLGALGHPERNVPPVI
                     HVAGTNGKGSTIAFLRAIL"
     gene            complement(257..1153)
                     /gene="accD"
                     /locus_tag="VFL49_06775"
     CDS             complement(257..1153)
                     /gene="accD"
                     /locus_tag="VFL49_06775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET7911715.1"
                     /translation="MNWISNLVRPKIRSFLRRETPENLWIKCPETGQLVFYKDVEANQ
                     FVIPSSNYHMRISAPQRLKAMFDGGEFEDVALPEVTLDPLKFRDERRYADRLKDARTK
                     TGLQDAVKVGVGRLEGQMVTIAVQDFDFMGGSLGMAAGEAVITGLETAVRRQTPFIMF
                     AASGGARMQEGILSLMQLPRTTVGVQMLREARKPYIVVLTNPTTGGVTASYAMLGDVQ
                     IAEPGALIGFAGPRVIEQTIREKLPDGFQRAEYLAEHGMVDMVVHRHKLRSTLAELCR
                     LLTRMPAPSGQYREQLPVPAHA"
     gene            complement(1211..2050)
                     /gene="trpA"
                     /locus_tag="VFL49_06780"
     CDS             complement(1211..2050)
                     /gene="trpA"
                     /locus_tag="VFL49_06780"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7911716.1"
                     /translation="MTTRIERRFAALKKQGRAALVTFTMAGDPDYATSLAIARELPKA
                     GADVIELGMPFTDPMADGPAIQAAGLRALKSGQQVSDTLRLVEEFRENEDETPIVLMG
                     YYNPIYIYGVEKFLGDAKSAGVDGLIVVDLPPEEDEELCIPTLKAGLNFIRLATPTTD
                     DHRLPTVLKNTSGFVYYVSITGITGAAAPDTGRVTTAVARIKRHTKLPVAVGFGVRTS
                     DHARAIAEGADGVVVGSALVDALRQSLDKNGKAGPGTVKAVTGLVSALAEGVRSVRRV
                     AAE"
     gene            complement(2047..>2288)
                     /locus_tag="VFL49_06785"
     CDS             complement(2047..>2288)
                     /locus_tag="VFL49_06785"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083569.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7911717.1"
                     /translation="HDMGRVKFLSATDEEAVAAFQLCSKLEGIIPALESAHALAKVPE
                     IAAKKSKDHLMVVNLSGRGDKDLTSVEAFLERKSR"
BASE COUNT          453 a          706 c          652 g          477 t
ORIGIN      
        1 tccagaattg cccgcagaaa cgcgatggtc gatcctttgc cattggtgcc ggcaacgtga
       61 atgaccggcg gcacgtttcg ctcggggtgt ccaagcgcgc cgagcagtcg ctgcacgcga
      121 ccgagtgaca gatcgatgcg ctttgggtgc agcgccatca gccgcgagac gatgtcttcg
      181 atttgactca tggcaacacg cttctcatca tcgggctatg cccggccatg acaaattata
      241 gggcatggct caacgttcag gcgtgcgccg gaacaggcaa ttgttctcgg tattggccac
      301 tgggtgcagg cattctcgtc aagagccggc acaattcggc aagtgtcgag cgcaacttat
      361 gccggtgcac gaccatgtcg accatgccat gctcggcaag atattcggca cgctggaagc
      421 cgtccggcaa tttttcacgg atggtctgtt caatcacgcg cggccccgca aagccgatca
      481 atgcacccgg ctcggcgatc tgcacgtcgc ccagcattgc gtacgaagcc gtcacaccgc
      541 ccgtggtcgg attggtcagt acgacaatat aaggcttgcg cgcttcgcgc agcatctgca
      601 cgccgacggt cgtacgtggc aactgcatca gcgacagaat tccttcctgc atgcgcgctc
      661 cgcccgaggc ggcaaacatg atgaacggcg tctgccggcg gacggcagtc tcaagccccg
      721 tgatcaccgc ttcaccggcg gccatgccaa gcgaaccacc cataaaatcg aaatcctgaa
      781 ccgcgatggt caccatctgg ccctcaaggc ggccgacgcc aaccttcacg gcatcctgca
      841 accctgtctt ggtgcgggca tctttcagac gatccgcgta gcggcgctcg tcacgaaact
      901 tgagcgggtc gagcgtcact tcgggcaggg caacatcttc aaactcgccg ccatcgaaca
      961 tggctttcag ccgttgtggc gcgctgatgc gcatgtggta gttcgaactc gggatgacga
     1021 actggttggc ctcaacgtcc ttgtaaaaga cgagctgccc cgtctccgga catttgatcc
     1081 agagattttc cggcgtctcg cgacgcagga agctgcgaat cttcgggcgt acgaggttgg
     1141 agatccagtt catgggtcgt ttctataggt acgattcggc aaaattataa ctgcctggcg
     1201 agccgaatgt tcactcggcc gccacgcgcc ggactgaacg cacgccctcc gccagtgctg
     1261 aaaccaggcc agttacggct tttaccgttc ccgggccggc tttgccgttt ttatcaagac
     1321 tttggcgcaa agcatccacc agcgcagacc cgaccacgac gccatcagcg ccttccgcaa
     1381 ttgcgcgagc gtggtcggat gttctcactc caaaccctac cgcaaccgga agcttggtgt
     1441 ggcgcttgat gcgcgccacc gccgtcgtca ctctgccggt gtccggcgct gcggcaccgg
     1501 taatgccggt gatggaaacg taatatacaa aacccgatgt gttcttgagc accgtcggga
     1561 ggcgatggtc gtcagtggtc ggcgtcgcga gacgaatgaa gttgagccca gctttcaggg
     1621 ttgggatgca gagttcttcg tcttcctccg gcggcaagtc cacaacgatc aagccgtcga
     1681 caccggctga tttcgcgtcg ccgagaaatt tttcgacgcc gtagatgtag atggggttgt
     1741 agtagcccat caggacgatt ggcgtttcgt cctcattctc acgaaattct tctacaagcc
     1801 tgagcgtgtc gctgacctgc tgaccagact tgagcgcacg caagccggcg gcctgaattg
     1861 ccggaccatc cgccatcgga tcagtgaacg gcatgcccag ttcgatgaca tcggctccgg
     1921 cctttggcaa ttcccgggcg atggcaagcg acgtcgcata gtcgggatcg cccgccattg
     1981 tgaaggtcac caacgcggcg cgcccctgtt tcttcagtgc agcgaagcgg cgttcgatgc
     2041 gggtcgtcat cgactctttc tttcaagaaa cgcctcgacc gacgtgagat ccttgtcgcc
     2101 gcggccggac aggttcacga ccatcaggtg atccttgctc tttttcgccg cgatctccgg
     2161 cactttcgca agcgcgtgcg cagactcgag cgcgggaatg atcccttcaa gcttgctgca
     2221 caactggaat gccgccaccg cttcttcgtc ggttgccgaa agaaatttca cacggcccat
     2281 gtcatgca
//
LOCUS       DASSRL010000331         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_953785, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000331 DASSRL010000000
VERSION     DASSRL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953785"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..711
                     /locus_tag="VFL49_06790"
     CDS             76..711
                     /locus_tag="VFL49_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911718.1"
                     /translation="MKKGTQGVWALVVAGAFTLAMGAATVSANEPGYGHGGGKDGYGG
                     GGHGMMGKHGMGMMHSSAGHLIRHLLKHEKEIGLTSDQVTKLKAIQLDLDKARIKAEA
                     DIQVAERELKALTDDEKSDLKAIEGKLRQSEDMQVALRMTSIKTRRDVLALLTPEQRA
                     KEKAEHEKMMQKHTEGGKGHGSSMPNPHGMMQGNPHQGGASPMPPANMKAQ"
     gene            735..1070
                     /locus_tag="VFL49_06795"
     CDS             735..1070
                     /locus_tag="VFL49_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7911719.1"
                     /translation="MRTQTITGVLALYLVVGLSTPGWSDDKEGKVTDLVKDAKVTAEQ
                     AIKTAMDKVPGTVVEAELEKKHGKTVWEVEILGADGHVTEVHIDAADGTVIDTEAKKG
                     EKKKEKKGK"
     gene            1076..2536
                     /gene="ilvD"
                     /locus_tag="VFL49_06800"
     CDS             1076..2536
                     /gene="ilvD"
                     /locus_tag="VFL49_06800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7911720.1"
                     /translation="MTKEPKRNSHELLTGPGRAPARAMLKAVGFTDDDLSRPIIGVAN
                     TWIEVMPCNFHLRRLSERVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDGVVALSGCDKTIPGTVMALARLNVPSLMLYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHASGKMTNSELKDLEDHACPGPGACGGQFTANTMAIAFEFLGMSPM
                     GRNGVPAMDTRKDDVAFECGKMVMDLLKNDLRPRQIITRKSLENAIAAVATTGGSTNA
                     VLHLLAIAYEAGLKLGIDDFDRINRKVPLLADLKPGGRFTAADLYAAGGTTLVAKRLL
                     DAGLLHANQPTVTGRTIGEEAKEAVETGGQQVLRPLMNPIKTTGGLVILRGNLAPDGC
                     VVKVAGHSMTHFRGPAKVYDREEDAFVAVKAGQIKDGDVVVIRYEGPSGGPGMREMLG
                     VTAAIVGAGLGDSVALLTDGRFSGATHGLMAGHVAP"
BASE COUNT          616 a          744 c          855 g          422 t
ORIGIN      
        1 agggagaagg cggcttgttt gtgcgataat gtcgtgtctc tgtctctcga ggacgtcaac
       61 acaaggaggt ttcgcatgaa gaagggaacg cagggagtct gggcgctcgt tgtggccgga
      121 gccttcacgc tggccatggg agccgccaca gtgtcggcca acgagcccgg ctacggccat
      181 gggggtggga aagacggcta tggaggaggc ggccatggga tgatgggcaa acatggcatg
      241 ggcatgatgc acagcagcgc cggccatttg atccgtcatc ttctcaagca tgaaaaggaa
      301 atcggcttga cgtccgatca agtgacgaaa ctcaaagcca ttcaactcga ccttgacaag
      361 gctcgcatca aagccgaagc ggacatccag gtggcagaac gcgaattgaa ggcgctgacc
      421 gacgacgaaa agtccgatct caaggcgatc gaagggaagt tgagacaaag cgaagacatg
      481 caggtggccc tgcgcatgac cagcatcaag actcgccgcg atgtcttggc tctgttgacg
      541 cccgaacagc gcgcgaaaga aaaggctgaa cacgagaaga tgatgcagaa gcacacagag
      601 ggcggcaaag gccacggcag tagcatgccg aatccgcacg gcatgatgca gggcaatccg
      661 catcagggcg gcgcctcgcc gatgcctccc gccaacatga aggcgcagta aggtagtcgc
      721 cgaaaggagg accgatgcgt acacagacaa tcaccggcgt gctggcgctc tacctggtcg
      781 taggcctgag cacgccaggc tggagcgacg acaaggaagg caaggtgacc gatctcgtca
      841 aagacgcgaa ggtgacggcc gagcaggcga tcaagaccgc catggacaaa gtgccgggca
      901 ccgtcgtgga agccgagctc gaaaagaagc acggcaagac cgtatgggaa gtcgagatcc
      961 taggcgccga cggccatgtg accgaggtcc acatcgacgc cgccgacggg acggtcatcg
     1021 acacggaagc gaaaaaaggc gagaagaaaa aagagaagaa ggggaaatag gcgagatgac
     1081 gaaagagccg aagcgcaaca gccacgaact gctgacggga ccaggccggg ctccggcacg
     1141 ggcgatgttg aaggccgtcg gctttaccga cgacgacctt tcccgtccga tcatcggcgt
     1201 cgccaacacc tggatcgagg tgatgccctg caactttcac ctacgccgct tatccgagcg
     1261 ggtgaaagcc ggcatccgcg ccgcgggcgg cacgcccatc gaatacaata cgattgccgt
     1321 ctccgacggc atttcgatgg gcaccgaggg catgaaggcc tcgctgatca gccgcgaggt
     1381 gatcgccgat tccatcgaat tggtggcgcg cggccatctg ttcgacggcg tcgtggcgct
     1441 gtcaggctgc gataagacga ttccgggaac cgtgatggcg cttgcccgtt tgaacgtacc
     1501 gtcgctcatg ttgtacggcg gatcgatcat gcccggacaa ttccaaggtc atgacgtgac
     1561 catccaggac gtcttcgaag ccgtgggcaa acatgcctcg ggcaagatga caaacagcga
     1621 gctgaaagat ctcgaagatc atgcctgtcc tggacccggc gcctgcggcg gccagtttac
     1681 ggcgaacacg atggcaatcg cgttcgaatt cctcgggatg tcgccgatgg gacgcaacgg
     1741 cgtccccgcg atggacactc gcaaggacga cgtggcgttc gaatgcggga aaatggtcat
     1801 ggatttgctg aaaaacgatc ttcgtccgcg gcagatcatc accaggaaat cgctcgagaa
     1861 cgcgatcgca gccgtggcga cgacgggcgg ttcgacgaac gccgtccttc atctgctggc
     1921 tatcgcctac gaagccggcc tcaagctcgg tatcgacgat ttcgaccgga tcaaccgcaa
     1981 ggttccgctg ctcgccgatt tgaaaccggg cggccgcttt accgccgcgg atctctacgc
     2041 ggccggcggc acgacgctgg tggcgaaacg cctgctggac gcgggtctcc tgcacgcgaa
     2101 tcaaccgacc gtgaccggcc ggaccattgg agaagaagcc aaagaggccg tcgaaaccgg
     2161 gggacagcaa gtcttgcggc cgctgatgaa tcccatcaaa acgacgggcg gtctggtaat
     2221 tctgagaggc aatttagcgc cggacggctg cgtcgtgaaa gttgccggcc actcgatgac
     2281 gcattttcgc ggaccggcca aggtctacga tcgagaagag gacgcgttcg tcgcggtcaa
     2341 ggccggccag atcaaggacg gcgatgtggt cgtgatccgg tatgagggac cgtccggcgg
     2401 tcccggcatg cgcgaaatgt tgggggtgac cgcagcgatc gtcggagccg gattgggcga
     2461 ctcagtcgca ctgttgaccg acggccgctt ttcaggcgcg actcacggcc tcatggccgg
     2521 tcatgtcgct ccgtaagccg tcagtggctg accgatcggc gcagtcaaga acggcgacat
     2581 cgtcgtgttc gacattccaa agcgccaact gaacgtcgaa ctgtccgtga aggaatt
//
LOCUS       DASSRL010000332         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1023929, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000332 DASSRL010000000
VERSION     DASSRL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023929"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFL49_06805"
     CDS             complement(<1..761)
                     /locus_tag="VFL49_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7911721.1"
                     /translation="MSEDRVKLREYHAPVWDEPVIMELGHPGRRGQVFFRKAGKVSGL
                     IPKGLARTEPPALPEMTEPEVQRHYLHLCQQTLGMVNISLFGTCTMKYNARASEAVMQ
                     LPQVADVHPLQPDDSIQGILQAIHEFDLGLRALSGMDQFIFQAGGGADAAYTNCVVTR
                     AYHQSRGELVRRNEIITSIQAHPCNAATAAAAGFKVVTLNLEKNGYPSVDALKAAISD
                     RTAALFINNPNDMGIYNPDIKEWVRLVQEAGGLCF"
     gene            complement(758..2164)
                     /gene="gcvPA"
                     /locus_tag="VFL49_06810"
     CDS             complement(758..2164)
                     /gene="gcvPA"
                     /locus_tag="VFL49_06810"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET7911722.1"
                     /translation="MGELTGHPWMGNALPDLKASMLKEIGAPSIDALFEQIPADHRIR
                     KALKLSDGIRSEALLRRRLTNMMAQNQSAATHLTFLGAGCWPHHVPAVVDEIVGRTEF
                     LTPVWGSPQSDFGRNQAWFEYASQLGELLNMEVVSLPVYSWGCAIGHALRMASRLTGR
                     REIIIAEIFDPERLSVVENYCEPIDMASHITIRRVKVEAKSGTIDIADLKRKVTDKTA
                     AVYFESPSYLGQFEINAAAIARIARQNGAETIVGVDPLTLGVARPPAEFGADIVVGPT
                     QPMGVHMNCGGGVGGFMASRDEERYVREYNGFLVSITETLKPGQFGFGLASAHQTSYG
                     MREQGKDWTGNSVYLWAIANAVYMSLLGPQGFREIGELILAQSHKAAKRLAKIKGVTI
                     RHPSGFFKEFVVDFTKTGKTVAAINKKLRREEIFGGKDLSKQLPEFGQSALYCVTEIH
                     TDEDIERLRSAIERAVRR"
     gene            2232..2999
                     /locus_tag="VFL49_06815"
     CDS             2232..2999
                     /locus_tag="VFL49_06815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911723.1"
                     /translation="MTDLSGKVVLLTGASKGIGAEMAGAIGAAGADLIAHYGDDRAGA
                     HEATAGLDKDRVKLIGADLKDKAAVDRLWADSLAWRGRVDVVINNAAVMRIAGGIEDD
                     DSAWDEVWDEALKVNVLAPVRLMRHAVRHYLQVGGGILITVSSWAAQRGPGNPALLAY
                     ASSKGAVLSATKTIARNYASKGILAYVIAPGVVRTRLSEQAAAAAGGEAVFTAGLAMG
                     EWVPPAELGSLAVFLASGACRHLSGATLDVNGASYIR"
     gene            complement(3120..3482)
                     /locus_tag="VFL49_06820"
     CDS             complement(3120..3482)
                     /locus_tag="VFL49_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911724.1"
                     /translation="MLAVMAGDGTTKGEVDIPLTDAQVQSQEISPPNRHCISHHALRF
                     PPIPVCQASTEDEGSAAIPDLDCNAQRTAERQLDECVDRSTSRSSAQGSTGSIEPVRA
                     NLTRHYTRNKGAVAESLQ"
BASE COUNT          642 a         1090 c         1180 g          753 t
ORIGIN      
        1 tagaagcaga gacctcccgc ctcctggaca aggcggaccc attctttgat gtcgggattg
       61 tagatcccca tgtcgtttgg gttgttgatg aagagcgccg ccgtgcgatc ggagatcgcg
      121 gccttcagcg cgtcgaccga ggggtatccg ttcttctcga gattgagcgt taccaccttg
      181 aagcccgcgg ctgcggccgt tgccgcgttg cagggatggg cctggatcga ggtgatgatc
      241 tcgttgcggc gcacaagctc accgcgtgac tggtggtagg cccttgtcac gacgcagttg
      301 gtataggctg catccgcgcc gccaccggcc tggaagatga attgatccat gcccgaaagc
      361 gcacgcagac cgagatcgaa ctcgtggatg gcctgcagga tgccctggat cgaatcgtcg
      421 ggctgcagcg gatgcacgtc ggcgacctgc ggcagctgca tcaccgcctc gcttgcgcgg
      481 gcgttgtatt tcatggtgca ggtgccgaac aagctgatgt tcaccatgcc gagtgtctgc
      541 tggcagagat ggaggtagtg gcgctgcacc tccggctcgg tcatctctgg caacgccggc
      601 ggttccgtgc gggcgagtcc ttttgggatc agcccggaga ctttgccggc cttacggaaa
      661 aaaacctggc cgcgccgacc aggatggccg agctccatta tcactggttc gtcccagacc
      721 ggcgcgtgat attcgcgcag tttcacccga tcctcgctca tcgccgcacc gcccgctcga
      781 ttgccgagcg cagtcgttcg atatcctcgt cggtatggat ctccgtcacg caataaagcg
      841 cgctttggcc gaattccggc agctgcttcg atagatcctt gccgccgaat atctcctcgc
      901 gcctcagctt cttgttgatg gcggcgacag tcttgccggt ctttgtgaaa tccaccacga
      961 actctttgaa gaagccgctc ggatggcgga tcgtgacgcc cttgatcttc gccaggcgct
     1021 tcgccgcctt gtgcgattgc gccaggatca gttcgccgat ttcgcggaag ccttgcggcc
     1081 ccagcagcga catatagacc gcattggcga tcgcccagag atagacggag ttgccggtcc
     1141 agtccttgcc ctgctcgcgc attccgtagg aggtctgatg cgcgctggcg aggccgaagc
     1201 cgaactgacc cggcttcaag gtctctgtga tgctgaccag aaaaccgttg tattcgcgca
     1261 cataacgctc ctcgtcgcgc gaggccatga acccaccgac gccgccaccg caattcatat
     1321 gaacgcccat gggctgggtc gggccgacca cgatgtctgc cccgaattcc gccggcggcc
     1381 tcgccacgcc gagcgtcagc ggatcgacgc cgacgatcgt ctcggcgcca ttctgccgcg
     1441 cgatccgcgc gatcgccgcg gcattgatct cgaactgccc gaggtacgag ggagattcga
     1501 aatagactgc cgccgtcttg tcggtgacct tgcgcttcag gtcggcgata tcgatcgtcc
     1561 cgctcttggc ttcgaccttc acccgcctga tggtgatgtg gctcgccata tcgatgggtt
     1621 cgcaatagtt ctcgacaacg gaaaggcgct cgggatcgaa aatctcggca atgatgatct
     1681 cgcggcggcc ggtgaggcgt gatgccatgc gcaaggcatg accgatggcg cagccccagg
     1741 aatagacagg caggctgacg acttccatgt taaggagctc gccgagctgg ctcgcgtatt
     1801 cgaaccaggc ctggttgcgg ccgaaatcgg attgcggcga tccccagacc ggtgtcagga
     1861 actcggtgcg gccgacgatc tcgtcgacca ccgctggcac gtgatgcggc cagcaacccg
     1921 cgccgagaaa ggtgagatgg gtagcggcgc tctggttctg cgccatcatg ttggtcaggc
     1981 ggcggcgcag cagcgcttcc gaccggattc cgtcagacag cttcagcgcc ttccggatgc
     2041 ggtgatcggc tgggatctgc tcgaacagcg catcgatgct aggagcacca atctccttga
     2101 gcatgctcgc cttcagatcc ggcagtgcgt tgcccatcca gggatggccg gtcaattctc
     2161 ccattgcgat cgctccctga gtattagtat acgtgttgta tacgaaatgg cgttgcgagg
     2221 cgagacgcat tgtgacagat ctctcgggca aggtcgtgct gctgaccggc gcttccaagg
     2281 ggattggcgc cgaaatggcc ggcgcgatcg gtgccgcggg tgccgacctc attgcccatt
     2341 acggcgacga tcgagccggt gcgcacgaag cgactgccgg tctcgacaag gatcgggtca
     2401 aactcatcgg cgccgatctc aaggacaagg cggccgtcga tcggctgtgg gccgatagcc
     2461 tggcgtggcg gggccgtgtc gacgtcgtta tcaacaacgc ggcggtgatg cggatcgccg
     2521 gtgggatcga ggacgatgat tccgcctggg acgaggtctg ggacgaagcg ttgaaggtca
     2581 atgtcctggc gccggtacgc ctcatgcgcc atgccgtgcg tcactatctg caggtgggcg
     2641 gcggcatctt gatcacggtt tcgagctggg ctgcgcagcg gggcccaggc aatccggctt
     2701 tgctggctta tgcgagttca aagggcgcgg tgctctcggc aacgaaaacg atcgcgcgga
     2761 actacgcaag caagggcatt ctcgcttacg tcattgcccc tggcgtggtg cgaacgcgcc
     2821 tctccgagca ggctgccgct gcggcgggcg gagaggcggt cttcaccgcc ggtctggcga
     2881 tgggggagtg ggtcccccca gccgagctcg gtagcctggc ggtctttctg gccagcggcg
     2941 cctgtcggca cctcagcggc gccacgttgg acgtgaacgg cgcgagctac atccgataga
     3001 gattgccgag gggtgtcttg tggacctggg acaccgggat aagcgttgga tctggctgag
     3061 ctgacatcag aagctcaaaa gcttgggctg ggctattaac gggcgcacgt gatcattggt
     3121 tactgaagac tttcagccac cgcgcctttg tttcgtgtgt aatggcgagt caaattggcg
     3181 cggactggtt caattgaacc agttgacccc tgcgcagatg agcgagaggt cgagcgatcg
     3241 acgcactcgt caagttgtcg ctcggcagtg cgctgtgcat tgcagtcgag atcgggaatt
     3301 gcggccgatc cctcgtcttc cgtacttgcc tggcaaacgg ggatcggagg aaagcgcaga
     3361 gcgtggtggc tgatgcaatg acgattcggc ggcgagattt cttgtgattg cacttgggcg
     3421 tccgttaagg ggatatccac ctcgcctttg gtcgtgccgt cgcctgccat gacggccagc
     3481 acgatcggat cggcaagaag gtcacggagt cgtggctctc cactaaactg tcaccggctc
     3541 cccggcatgg ttggcatcct ccatcgcgcc aggctcctcc ccgccgttcg caggatgctt
     3601 cagcggtttc agcgtctgct taagttgtgt gcaggttgcg atgttccgct catggacttc
     3661 gagca
//
LOCUS       DASSRL010000333         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_988571, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000333 DASSRL010000000
VERSION     DASSRL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988571"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(443..1810)
                     /locus_tag="VFL49_06825"
     CDS             complement(443..1810)
                     /locus_tag="VFL49_06825"
                     /inference="COORDINATES: protein motif:HMM:NF005460.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7911725.1"
                     /translation="MGWTECENGPTVPPDTIQKAVPMARNSARERLEAAFARIDDPEG
                     EGARACLIVYREQARAAADAADARTKSGISLGPLDGTIVSIKDLFDVAGEVTRAGSRI
                     LAEERKPAAADAPVVRRLRAGGAIIVAKTNMSEFAYSGIGANPHFGTPGNPADRKRVP
                     GGSSSGAVVSAADGMCEIAIGTDTGGSCRIPAAICGIVGYKPSRQRIPTEGAFPLSYS
                     IDSIGPIARTVEACAKADAVMAAENFAPLEPAQLAGLRVGIAQGEPAENLDATVSKRF
                     AGAIDQLRKAGAQLSEEKISLFADMADVNRKGGVAPAEAFAIHRELMTRRAQDIDPNI
                     RIRLERARNINAADYIDMVQQRARLIRLMGMRLADIDVLVLPATSIVAPTMQEVAPAD
                     EFARRNAMLLRNTAIVNFFDLCAISVPIPRESDLPVGLMLVARNGHDHRLFRVAMAVE
                     RLFGG"
     gene            1887..>2088
                     /locus_tag="VFL49_06830"
     CDS             1887..>2088
                     /locus_tag="VFL49_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911726.1"
                     /translation="MTAKGKHSPPGKGNIYAKANEVIVKITGDDTAQTFEVVEENCKP
                     GFQSRAHYHIKAYETFYVFEGSA"
BASE COUNT          410 a          654 c          636 g          388 t
ORIGIN      
        1 atatgtacaa tagccgccct gacgacggtc ttcagagcag cgggttgaga ctttcgtcgg
       61 ctgctggagg ccgtcatgga tcggaccgga cgatccggag ggccaagcac ggagatcagc
      121 cctaatctga cggatttcaa tcttcaaatc gcggccggac gatggtgagt atgcctacgc
      181 ggcgcacgcg ctcggactgt tgcgcccgta ccgtaagtgg ccctcgtatg tccgcctgga
      241 cctgccgcct tattgcggca agaccgaccc aaagattgtt ccgcctcgtt ctcgaacccc
      301 atgaggaagg gcctggcgcc gattccgaag agaagcacga gcccgcgtgt gacatcgaca
      361 ctggaccgat ggatagcctg aaagcgcccg gaacggccga gcggacatcg atgcatgccg
      421 ctttcctgcg caaccgcatc gcctaaccgc cgaacaatcg ctccaccgcc atcgcgacac
      481 ggaacagccg gtgatcgtgg ccattgcggg ccaccagcat caggccgacc ggaagatcgc
      541 tttcgcgcgg gatcggcacg gaaatggcgc agaggtcgaa gaaattcaca atagccgtgt
      601 tgcgcaaaag catcgcgttc cggcgcgcga attcgtcggc cggcgccacc tcctgcatcg
      661 tcggtgcgac gatcgaggtg gccggcaata ccagaacgtc aatgtcggcg agcctcatgc
      721 ccataagccg aatgaggcga gcacgctgct gcaccatgtc gatgtagtca gccgcgttga
      781 tgttgcgtgc ccgctccagc cggatccgga tattcgggtc gatatcttgg gcgcgacgcg
      841 tcataagctc gcggtgaatc gcaaaggctt ctgcgggcgc cacgccgccc ttgcggttga
      901 cgtcggccat gtctgcaaac agcgagatct tctcttccga cagttgcgct cctgctttgc
      961 gcaattgatc gatcgctccg gcgaagcgct tcgataccgt cgcgtcgaga ttctccgccg
     1021 gctctccctg agcgatgccc acacgaagac cggcaagctg cgcgggctcg agtggcgcaa
     1081 agttttcagc cgccatgacg gcatcggcct tcgcacaagc ttcaacagtt ctggcgatcg
     1141 ggccgattga atcgatgctg tatgacaacg ggaaagcgcc ttcggtcggg atgcgctggc
     1201 ggctgggctt gtagccgacg ataccgcaga tcgccgccgg gatacggcat gagccgccgg
     1261 tatctgtgcc gatcgctatt tcgcacatgc cgtcggcggc tgaaaccacg gcgcccgacg
     1321 acgagccgcc tggaacacgc ttgcggtcgg cggggttgcc cggcgttccg aagtgcggat
     1381 tggcgccgat acccgagtag gcaaattcgc tcatgttcgt tttcgcgacg atgatcgcgc
     1441 cgccggcacg cagcctgcgg acgacaggcg catcagcggc ggccggtttt cgttcctccg
     1501 caaggattct ggatcctgcc cgcgtcactt cacctgccac gtcgaagagg tccttgatac
     1561 tcacaatcgt gccgtccagc ggaccaagtg aaattccgga cttcgtgcgc gcatcggccg
     1621 catcggcggc tgccctggcc tgctcgcgat agacgatgag gcaggcgcga gcgccttctc
     1681 cttcaggatc gtcgatgcgg gcaaacgcgg cctcaagccg ctcgcgggcg gaatttcgtg
     1741 ccatagggac tgccttttgg atcgtatccg gtggaactgt cgggccattt tcgcattccg
     1801 tccagcccac cgccaatgtg tccctctgtt cagtgggggc gtgcctatgc aatcaattgg
     1861 cgaggaaacg atcgagagaa attcccatga ccgccaaagg caagcactcg ccgccgggca
     1921 aaggcaacat ctacgccaag gccaacgagg tcatcgtgaa gatcacgggt gatgacaccg
     1981 ctcagacctt cgaagtggtc gaggagaact gcaaacccgg ctttcagtcg cgagcccact
     2041 atcacatcaa ggcctacgaa acgttctacg tgttcgaagg cagcgcgg
//
LOCUS       DASSRL010000334         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1461082, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000334 DASSRL010000000
VERSION     DASSRL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461082"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..863
                     /locus_tag="VFL49_06835"
     CDS             111..863
                     /locus_tag="VFL49_06835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911727.1"
                     /translation="MATSQTNQPRRTLLLTGASRGIGHATVKRFSAAGWRVITCSRHP
                     FPENCPWEAGPEDHIQVDLSDEDNTNEAIAEIKRRLDNKELHALVNNAAISPKAEGGK
                     RLSTLDTSNDDWHHVFQVNFFAPIMLARGLMDELKAAKGSVVNVTSIAGSRVHPCAGA
                     AYSTSKAALAALTREMASDFGRLGIRVNAIAPGEIDTAILSPGTQKIVEQIPMHRLGT
                     TDEVAKIIYVLCTDTSSYVNGAEIHINGGQHV"
     gene            1629..1913
                     /locus_tag="VFL49_06840"
     CDS             1629..1913
                     /locus_tag="VFL49_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911728.1"
                     /translation="MKTVIATIIVLAAISVASPAMAQSFDADNGTGNIAATAGWTGRS
                     QTRTVREYPGVSAYAMSPRRKAGFAEEGRGTIDTTTGGGSPGYNEMLRNW"
     gene            2155..>2580
                     /locus_tag="VFL49_06845"
     CDS             2155..>2580
                     /locus_tag="VFL49_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET7911729.1"
                     /translation="MATAKYVRRKDDVPSSHEVSLQAKLRDAIVAKLVYSLGKTKASA
                     TDRDWYQATALAIRDRVIDIWVQAKGGAKRERKKRVYYLSIEFLIGRLLFDNLSNLRL
                     VETTRAALSEFGVDLDRLREIEPDPALGNGGLGRLAACFM"
BASE COUNT          554 a          817 c          735 g          474 t
ORIGIN      
        1 gaattgcatt tacgcgcgac aagccgggcg cagcatggag caagacgcgg ctctacccgt
       61 aagctagcat catggaacga tcgccaagct agaacgattc caggagcgaa atggcgacat
      121 cgcagacgaa ccagccgcgc cgcacgctgc tcctgaccgg agcgagccgc ggcatcggcc
      181 atgcaacggt gaaacgattt tccgcggcag gctggcgcgt catcacctgc tcgcgacatc
      241 cgtttccgga aaattgcccg tgggaagcag ggccggaaga ccacatccag gtcgatctct
      301 ccgatgaaga caacacgaac gaagcgatcg cggaaatcaa gcggcgcctg gacaacaagg
      361 aactgcatgc gctggtcaat aacgccgcaa tctctcccaa ggccgagggc ggcaaacggc
      421 tctcgacact cgatacaagc aacgacgact ggcaccacgt cttccaggtg aatttctttg
      481 ccccgatcat gctggcgcgc ggcctgatgg atgagctcaa ggctgcgaaa ggttcggtgg
      541 tgaacgtaac ctcgatcgcc ggctcgcgcg tgcatccgtg tgcgggcgcc gcctattcca
      601 cgtcgaaagc ggcgcttgcc gcactgacgc gcgagatggc aagcgacttc ggccggctcg
      661 gcatccgcgt caacgccatc gcgcctggcg aaatcgacac cgcgatcctg tcgccgggca
      721 cgcaaaagat cgtcgagcag attccgatgc accggctcgg caccaccgac gaggtcgcca
      781 agatcatcta cgtgctgtgc accgacacaa gctcttacgt gaacggtgcc gagattcaca
      841 tcaacggcgg ccagcacgtg tgaaccgtga cgtcataaat acgattcgct tttcaaacag
      901 cccagacacg tcgacagcca acgctccgcc gcccggattt ttgctcaggc gccgggagcc
      961 gcctgcattt ccttgtccgc gatcgcccgg aaagagaacc gagggcgcgc gagacgccag
     1021 gggtccaatt ggacccgcgg gcctcgacgc ctcgcgacat cgaggtctgt cgaagtcctt
     1081 atgtgcccgc cgctcgccac gctgccacca ccgctcatct ccccgcaagc ggggatccag
     1141 ggcacacggc aacgcccctc atccgtcatg gccggacttg ttccggccat ccacgtcttg
     1201 cggcgagaag aaaggcgtgg atgcccggca taaagccggg catgacgccg aaagtgtcgc
     1261 gcgtccgaca tccgattcac ttttcaaaac agcccggcat caagccaacg ctccgcccct
     1321 gcaccgcatc tccgatgcaa ggtcatcctc ccggccaccg ctccccgccc cgcgtctgga
     1381 gacgctgatc agacacccct cggtaacggg gcggggtgtc ctatgactac tatcctatag
     1441 gaatattgtc aagatctgga aacgatattt ttcctcaaaa tgtgtgcggc ccgttctccg
     1501 aacattcacg gtcccgaccg cttccatcgc cccgactgtc ggctggttca cacaggcggg
     1561 cggttcgaag ggcgcatact gcccgccatg cggtgatatc ggtcaccggt aaaggatgga
     1621 gcatcagcat gaagacggtt atcgccacga tcattgtcct tgccgcgata tcggttgcct
     1681 cgccggcaat ggcgcaatcc ttcgatgcgg acaacggaac cggcaacatc gccgcgacag
     1741 caggatggac cggccgttcc caaaccagaa cagtccgcga atatccaggc gtcagtgcct
     1801 acgcaatgtc accgcgccgc aaggcaggtt tcgccgagga aggccgggga actatcgaca
     1861 cgacgacggg cggcggcagc cctggttaca atgaaatgct gcgcaactgg tgagtcgttt
     1921 cacatagaaa aggaggcggc tcaacgcccc tccttcacgg tacgcatttt gcatagcgga
     1981 acgatctgct tgatttacga ttttctcttt cggtggatga tcacattttc tggcttgtat
     2041 gtctgttcgc tccgcgcgac gcatctattt gtgtcatgaa gacgcggccg gatgccgcat
     2101 ggaacttttt atcgattgcg cataggcacc cgaccgtctt tcttcgagcc gcaaatggcc
     2161 acagcgaaat acgtcagacg caaagacgat gtcccttcct ctcatgaggt ttcgcttcag
     2221 gcgaaactcc gcgacgccat tgtggcgaag ctcgtctata gtctggggaa gacgaaagca
     2281 tcggcgacgg accgcgattg gtatcaagcc acagcattgg caatacgcga ccgcgtcatc
     2341 gacatttggg tgcaggccaa aggcggcgcg aagcgggagc gcaaaaagcg cgtctactat
     2401 ctctcgatcg aatttctgat cggccgcctg cttttcgaca atctcagcaa tctgcggctc
     2461 gtcgaaacca cgcgcgcggc gttgagcgaa ttcggcgttg acctcgatcg cctccgggag
     2521 attgagcccg atccggcact tggcaatggc ggcctcggac ggttggccgc ctgcttcatg
//
LOCUS       DASSRL010000335         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1286085, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000335 DASSRL010000000
VERSION     DASSRL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286085"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..657)
                     /gene="hisH"
                     /locus_tag="VFL49_06850"
     CDS             complement(7..657)
                     /gene="hisH"
                     /locus_tag="VFL49_06850"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET7911730.1"
                     /translation="MSVAIVDYGSGNLHSAAKAFERAARESGKAQPIIVTGDPDTVAR
                     ADRVVLPGVGAFADCRRGLDGISGMIDALEQTVRKKGKPFFGICVGMQLMAERGREYR
                     VTPGFGWIPGEVDRITPSDPKLKIPHMGWNTLNMLTDHPVVAGIPLGLNGLHAYFVHS
                     YELKAARAADLVAQADYGGPVTAMVGRDNMIGTQFHPEKSQRLGLALIANFLKWKP"
     gene            complement(654..1163)
                     /locus_tag="VFL49_06855"
     CDS             complement(654..1163)
                     /locus_tag="VFL49_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HET7911731.1"
                     /translation="MPTYTVHAPQAVPTSDASRATERFVFVRDGFHFWAFVLAPLWLL
                     LHRLWLALLVYIVGYGILGVGLAFARVSSNIQLIVALLIALLIGFEASSIWRWTLARR
                     GWATLGFVVAEDAETAERRFFAEWQKRAAQAPPAPVPAEPKYSTPVRRGPPAASDVIG
                     LFPEPGGQR"
     gene            complement(1264..1854)
                     /gene="hisB"
                     /locus_tag="VFL49_06860"
     CDS             complement(1264..1854)
                     /gene="hisB"
                     /locus_tag="VFL49_06860"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET7911732.1"
                     /translation="MRKGAVKRKTKETDVEVEIDLDGAGRASIATGIGFLDHMLDLLA
                     RHSRIDITVKAKGDLHIDHHHTTEDVGIALGQAVKQALGDMKGLTRYADVHVPMDEAL
                     TLVAIDISGRPFLVFKAEFVRDKVGTFDTELVQEWFQAFAMNAGVTLHAEVLYGTNDH
                     HIAESCFKGLARALRTAVTIDPRARDEVPSTKGSLG"
     gene            1976..>2488
                     /gene="hslV"
                     /locus_tag="VFL49_06865"
     CDS             1976..>2488
                     /gene="hslV"
                     /locus_tag="VFL49_06865"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET7911733.1"
                     /translation="MSPNELPSWHGTTILTVRKDGTVVIGGDGQVSIGQTIVKSNAKK
                     VRRLGKGDVIGGFAGATADAFTLFERLEAKLEQYPGQLTRAAVELAKDWRTDRYLRRL
                     EAMMIVADISVSLVLTGTGDVLEPENGVMGIGSGGNYALASARALLDGPLDAEQIVRR
                     SLDIAADICVF"
BASE COUNT          503 a          762 c          732 g          491 t
ORIGIN      
        1 aaagaatcac ggcttccact tcaaaaaatt cgcaatcaga gcgagtccaa gccgctggct
       61 tttttccgga tggaattgag tgcctatcat gttgtcacgt ccgaccatgg ccgtaacagg
      121 cccgccgtaa tcggcctgcg cgacaagatc tgcggctctt gcggccttca gctcatagga
      181 atgcacgaaa tatgcgtgca agccgttcaa accgagcggg atacctgcga ccaccggatg
      241 atccgtcagc atgttgagtg tgttccaccc catgtgcgga atcttcagtt tgggatcgga
      301 gggcgtgatg cggtcgacct cccccggtat ccaaccgaag ccgggcgtta cccgatattc
      361 gcgtccgcgc tcggccatca attgcatacc aacgcagatt ccgaagaatg gtttgccttt
      421 tttgcgcacg gtctgctcaa gcgcatcgat catcccggaa attccatcga gtccccggcg
      481 gcaatctgcg aacgcgccca cgccaggcag aacgacacga tcggctcgcg cgacggtgtc
      541 gggatcgcca gtgacgatga tcggctgggc cttcccgctt tcgcgtgctg cacgctcgaa
      601 cgccttggca gctgaatgca gattgccgga gccgtagtcg acaattgcaa cgctcaccgc
      661 tggcctccag gctccggaaa caagccgatg acatctgatg ctgccggcgg gcctcgccgc
      721 accggcgtcg aatatttcgg ctcagccggt acgggggctg gaggcgcctg cgcggcgcgt
      781 ttttgccact ccgcaaaaaa acggcgctcc gctgtttcag cgtcctcggc tacaacaaaa
      841 ccaagcgtcg cccagccgcg gcgagccaaa gtccatctcc agatgctgga cgcctcgaac
      901 ccgatcagca gcgcaataag cagcgcgacg atcagttgga tatttgacga cacgcgcgca
      961 aaagcgaggc cgacgccgag gattccatag ccaacgatat aaacgagcaa tgccagccag
     1021 agccgatgca gcagcaacca cagcggcgcc agtacaaacg cccagaaatg gaatccatca
     1081 cgcacgaaca cgaaacgctc ggtcgcgcgt gatgcatcgc tcgttggcac ggcttgtggt
     1141 gcgtgcacag tgtaggtggg cattgcacgt cctcgtactc aaactcatcg tggccggctc
     1201 gtcggcgccg ttctaccgag aacggacagc gaaaatcggg ttactcccga cctccggcag
     1261 gcgtcatccg agtgaaccct tcgtggacgg cacttcgtct ctggcgcgcg gatcaatggt
     1321 cacggccgtc cgcaatgcgc gcgccaagcc tttaaagcag gactcggcga tatgatggtc
     1381 attcgtgccg tacagcacct cggcatgcag cgtaaccccg gcattcatcg cgaaggcctg
     1441 gaaccattcc tgcaccagct cggtgtcgaa cgtgccgacc ttgtcacgca cgaactcggc
     1501 cttgaaaacc aggaacggcc gaccggaaat atcgatcgct accagcgtaa gcgcctcgtc
     1561 catcggcacg tgcacgtcgg cgtaccgcgt gaggcccttc atgtctccga gtgcttgctt
     1621 gacggcctgt ccaagcgcga taccgacatc ttcggtggta tggtgatgat cgatgtgcag
     1681 gtcgcctttg gctttgaccg tgatgtcgat gcgtgaatgc cgcgcgagca ggtcgagcat
     1741 atggtccagg aagcctatgc cggtcgcaat cgacgcgcgg ccggctccgt cgaggtcgat
     1801 ttcaacctcg acgtctgttt ccttggtctt gcgcttgacg gcacccttgc gcatgcagat
     1861 ggcctttgtt cgcgcgcctt ttatcagggg tttgcgggat tcgccaccgc gtagcgaacg
     1921 gtttgcatat acggcgcatc gtgactagat gacgcggatt gtggggctta ggcgcatgtc
     1981 accgaacgaa ctcccctcct ggcatggcac gacaatcttg accgttcgca aggacggcac
     2041 ggtggtcatc ggaggcgacg gccaggtttc gatcggccag accatcgtca aatccaatgc
     2101 aaaaaaggtc cggcgccttg gaaagggtga cgtcatcggt ggctttgccg gtgccactgc
     2161 cgacgcattt accttgtttg agcggctgga agccaaactt gagcaatacc cgggccagct
     2221 cacccgggcg gccgttgagc tcgccaagga ttggcgaacc gaccgttacc tgcgccgtct
     2281 cgaagccatg atgatcgtcg ccgatataag tgtttcattg gtcctcaccg gaaccggtga
     2341 cgtactggag cccgaaaatg gcgtcatggg aatcggctcc ggcggcaatt atgcgctcgc
     2401 gtcggcacgg gcgctgctgg acgggccgct cgacgccgag cagatcgtgc gcaggtcgct
     2461 ggacattgcg gctgatatct gtgttttc
//
LOCUS       DASSRL010000336         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_131383, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000336 DASSRL010000000
VERSION     DASSRL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131383"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFL49_06870"
     CDS             <1..352
                     /locus_tag="VFL49_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HET7911734.1"
                     /translation="NVTRFPYEIARLAQDIAGGLVVTMPSEKDLQNNEIGPLIRKFLQ
                     GRDGVEVTDRMRILRLIENMTIGRNAVGYLTESLHGAGSPQAQRIQIARGMKIEEKKA
                     RARHLAGVAQTGSK"
     gene            complement(334..714)
                     /locus_tag="VFL49_06875"
     CDS             complement(334..714)
                     /locus_tag="VFL49_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HET7911735.1"
                     /translation="MPVNKKHDEFHTLDLNVGWETPPGYPQGIEQKILSGALDETNRR
                     GTRTRLLRFAPGVFTTKPFVHDYWEEVFLVSGDLTVGNDAQGNGGKSFPPNTYAVRPP
                     GAYHGPFKSNGGCMLLEIHYFDPV"
     gene            complement(721..1251)
                     /locus_tag="VFL49_06880"
     CDS             complement(721..1251)
                     /locus_tag="VFL49_06880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7911736.1"
                     /translation="MLDTRRFRDALGQFPTGVTIITALTAGGDRLGMTISSFNSLSLD
                     PPLVLFSILRKANSFAAWQQAKRYAINVLNESQEELSNKFARPLSEKWIGQTVLTGKT
                     GLPLLPNAVIAFECEAYGRHDGGDHEIFVGRVVELHENQAKRGHPLVFFGGQYRRLGS
                     VEANAPADIVNVLHGW"
     gene            1321..2001
                     /locus_tag="VFL49_06885"
     CDS             1321..2001
                     /locus_tag="VFL49_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HET7911737.1"
                     /translation="MLAFERHSNGETDRAELVPKSLIVAGWTGRDEKALHHHIEELAA
                     IGVPRPSSVPVFYRISTANLTQAAQLEVLGPDTSGEAEPVVIALQDGLWLGVGSDHTD
                     RKAETMGIALSKQLCAKVVGKVLWRLDEVAPHWDQLILRAHVTIDGKRLKYQEGPLSA
                     MRNPADLMSRIGGEARFAAGTIMFCGTLGAIGGIRPAAQFTALLEDPVLGREMRCDYR
                     IDALPVVR"
BASE COUNT          397 a          612 c          623 g          396 t
ORIGIN      
        1 caatgtcacg cgctttccct acgagatcgc gcggctggca caggatatcg ccggcggcct
       61 tgtcgtcacc atgccgtcgg agaaggacct gcagaacaac gaaatcggtc cgttgatccg
      121 caagttcctg caagggcgcg acggcgtcga ggttacggac cgcatgcgca tcctgcggct
      181 gatcgagaac atgaccatcg gccgcaacgc cgtcggctat ctgaccgaaa gcctgcacgg
      241 cgccggctcg ccgcaggccc aacgcatcca gatcgcgcgc ggcatgaaga tcgaggagaa
      301 gaaggcgcgc gcccggcacc tcgccggcgt cgctcagacc ggatcgaagt agtgaatttc
      361 caggagcata cagccgccgt tcgacttgaa cggcccgtga taggcgccag gtggacgaac
      421 ggcataggtg ttgggcggaa atgattttcc gccatttcct tgtgcgtcgt tgcctacggt
      481 caggtcgccc gatacgagga agacctcctc ccagtaatcg tgcacgaagg gcttcgtcgt
      541 gaacacgccc ggcgcgaaac gcaggagtcg cgtacgcgtg ccgcgcctgt ttgtctcgtc
      601 gagcgcgccc gacaggatct tctgctcgat gccttgtgga tagccgggcg gcgtttccca
      661 gccgacattc aggtcgagag tgtggaattc gtcgtgtttc ttgttgactg gcatcgtcct
      721 tcaccatcca tgcaggacat tgacaatgtc ggctggcgca ttcgcctcga cggagcccag
      781 gcgccgatac tggccgccga agaataccag cgggtggccg cgcttcgcct gattttcatg
      841 cagctcgacg acgcgtccga caaagatttc gtggtcaccc ccatcgtggc gcccgtaggc
      901 ttcgcactcg aatgcgatca cggcattcgg caataatggc aggccggtct tgccggtcaa
      961 caccgtctgc ccgatccatt tttcgctcag cgggcgcgcg aacttgttgg acagctcctc
     1021 ctggctctcg ttcaggacgt tgatcgcgta gcgcttggcc tgctgccagg cggcgaaact
     1081 gttggccttg cgcaaaatgc tgaacaggac gagaggcgga tcgagcgaca gcgaattgaa
     1141 cgaactgatg gtcataccaa gccggtcgcc gccagccgtc agcgctgtga taatagtgac
     1201 gccggtcgga aactgaccga gcgcgtcacg aaaacgtcgg gtgtccaaca cggatttccg
     1261 ccggtgaatt ttcttcggtg atcatgtacc aagtgcctgt cgccaatcaa ggaacaagcc
     1321 atgctcgcct ttgagcgcca ttcaaacggt gaaactgatc gcgccgaact tgtgccgaaa
     1381 tcgctgatcg tcgcgggttg gaccggccgc gacgagaaag cgctgcatca tcacatcgag
     1441 gagctggctg cgatcggcgt gccgcgcccg tcgtcggtac cggtgttcta ccgcattagc
     1501 acggcaaatc tcacgcaagc cgcgcaactg gaggttcttg gacccgacac ctcgggcgag
     1561 gccgagccgg tggtcattgc gctgcaggat ggattatggc ttggcgtcgg ctcagatcac
     1621 accgaccgca aggcggaaac gatgggcatc gcgctatcga aacagctttg cgcgaaggtt
     1681 gtcggcaaag tcctctggcg tctcgatgaa gttgcccctc attgggacca attgatcttg
     1741 cgcgcccatg tgaccatcga cggcaaacgc ctgaaatacc aggagggccc gctgtcggcc
     1801 atgcgcaatc ccgccgacct gatgtcacgc atcggcggtg aagcaagatt tgctgcgggt
     1861 acgatcatgt tctgcggaac gctcggggcc atcggaggca ttcgcccggc ggcacagttt
     1921 actgctctgc tcgaggatcc ggtcctcggc cgcgagatga gatgcgacta ccggattgat
     1981 gccttgccgg ttgtccgctg atgtggatca accggtcgca ttcgataa
//
LOCUS       DASSRL010000337         3942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_866396, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000337 DASSRL010000000
VERSION     DASSRL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866396"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="hemN"
                     /locus_tag="VFL49_06890"
     CDS             complement(<1..993)
                     /gene="hemN"
                     /locus_tag="VFL49_06890"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET7911738.1"
                     /translation="MSNIKSIRPDIIARFEGQRAPRYTSYPTAPHFTHAFDDPEYQTL
                     LTALPSDSKLSLYLHVPFCQTMCWYCGCHTRIVASHSSIEAYLHALEVEIRMVAAALP
                     QRMTVNHIHWGGGTPTIMAPNAFLNLMNLLRECFECASDAEIAVEIDPRRVTDKMIRT
                     LGQCGVTRASLGVQSFDPAVQKAINRIQTYEQTGRVTHGLRRMGIEVVNFDLIYGLPY
                     QTVASCEDTVAKALQLEPDQLSVFGYAHVPWMKKHQRMIETSALPAPRERLAQFAAIA
                     DRLVDAKYRRIGLDHFARPADRLARCLDAGTLHRNFQGYTSDQCTTLIGFGASAV"
     gene            complement(1048..1461)
                     /locus_tag="VFL49_06895"
     CDS             complement(1048..1461)
                     /locus_tag="VFL49_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7911739.1"
                     /translation="MQNTDDQRIMLIDQDEVVRDSLKILIESHGMQVEEFRSPTDFLE
                     RNDFGRQGCVVIGSNRHTVDSLDLLNTLRKRGTRLPVVFIVGGGNALTRTTALAFGAA
                     AYLDRPIEETTLIRTIRDVPGRAPVAGPVAQARPH"
     gene            1736..2788
                     /locus_tag="VFL49_06900"
     CDS             1736..2788
                     /locus_tag="VFL49_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HET7911740.1"
                     /translation="MRLRFQRGRPDNLETLHPAYFGMVMATGIVAIATMLCGVPIVPI
                     VLFWLNAAIFAVLLVATGMRAVRYPGAFLADIRDHNRGVGFFTLVAAFGIFGNELVIQ
                     MQAAVAAVIFWAMTAVLWFVVTYGILAVLTVKPDKPGLTHGINGGWLVIVVATQSISI
                     LTVLILPAGVSADFHARLMFAALALWLGGGALYMWLMTLIFFRYTFRRMSAEDLTPPY
                     WINMGAVAISTLAGATLLREAALSPTVIELAPFVKGFTLLFWAIGSWWIPMLLVLGVW
                     RYIMCGVPFAYDPLYWGGVFPLGMYSVCTRRLAEVLNISFLMPVSTAFVVLAALAWLA
                     TFAGLIDSRLGVKLRA"
     gene            2838..>3942
                     /locus_tag="VFL49_06905"
     CDS             2838..>3942
                     /locus_tag="VFL49_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7911741.1"
                     /translation="MSHFLERLTYLSRRREAFADGHGELRDEDRTWEEGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKSGVVTWETQQTDYPRTRPGMPNHEPRGCSRGASYSWY
                     LYSASRVKYPMVRGRLVKAWREARKTLAPVEAWAAVAGNAATATSYKSMRGRGGFVRS
                     TWDEVSEIIAAANVHTIKTWGPDRVVGFSPIPAMSMVSYASGARYLSLIGGSLLSFYD
                     WYCDLPPSSPQTWGEQTDVPESADWYNSAYIIAWGSNVPQTRTPDAHFFVESRYNGTK
                     VVAITPDYSEVAKLSDLWLHPKQGTDAAFAMAMGHVILREFFLDRQVPYFQDYSRRYT
                     DLPMLVRLRRDGARWVPDRYVRQSDLQDSPKSDR"
BASE COUNT          707 a         1168 c         1238 g          829 t
ORIGIN      
        1 tacagccgac gcgccaaagc cgatcagtgt cgtgcactga tcactggtat agccctggaa
       61 attgcggtgg agtgtgccgg catccaggca tcgcgccagg cgatcggcgg gcctggcaaa
      121 atggtcgagg ccgatacgcc ggtatttcgc gtccacgagc ctgtccgcga ttgcggcgaa
      181 ctgcgccagc cgctcgcgcg gagccggcaa ggctgacgtt tcgatcatcc gctgatgttt
      241 cttcatccac gggacatgtg cgtagccgaa caccgagagc tgatcgggct ccagctgcag
      301 cgctttcgcc accgtatcct cgcaggacgc gaccgtctga tacggcagcc cgtagattag
      361 gtcgaaattg accacctcga tccccatgcg gcgcagccca tgcgtgacgc ggccggtctg
      421 ctcatacgtt tgaatgcggt tgatcgcttt ctgcacggcc ggatcaaacg actgcacccc
      481 caggcttgcg cgcgtgacgc cgcactggcc cagcgtcctg atcattttgt cggtgacacg
      541 gcgcggatcg atctcgacag cgatctcggc gtcgcttgcg cactcgaagc actcgcgcag
      601 caggttcatc agattcaaga acgcattggg cgccatgatc gtgggcgtgc cgccgcccca
      661 atggatgtgg ttgacggtca ttcgctgcgg caaggcggct gccaccatcc ggatttcgac
      721 ttccagcgcg tggagatagg cttcgatcga gctgtggctc gcgacgatgc gggtgtggca
      781 gccgcagtac caacacatgg tctggcagaa cggcacatgc aggtagagcg acagcttgct
      841 gtcggacggc aaggcggtca gcagcgtctg atactccgga tcgtcgaagg cgtgggtgaa
      901 atgcggcgcc gtgggatagc tggtgtagcg cggcgcgcgc tggccttcga agcgcgcgat
      961 gatatccggg cggatggact tgatgttgct catgcacctc cagctggctt gatttcaggc
     1021 cggtatcatg tcacgggctt cagcgcctca atgcggtctt gcctgagcga cgggaccggc
     1081 gaccggcgct cgtcccggga cgtccctgat ggtgcgaatc agcgtcgttt cctcgatggg
     1141 cctgtccaga taggcggctg ctccgaatgc caacgccgtc gtcctggtca atgcattgcc
     1201 cccaccaacg atgaagacga ccggcagccg agttccgcgc ttgcgcagcg tgttaaggag
     1261 atcgagactg tcaacggtgt gccgattcga cccgatcaca acgcaacctt gccggccaaa
     1321 gtcattgcgt tcgagaaagt ccgtcgggga tctgaattcc tccacttgca tgccatgaga
     1381 ttcgatcagg atcttgagcg aatcccgtac gacctcatcc tggtcgatga gcatgatgcg
     1441 ctgatcgtct gtattctgca cagatcctcc ggcaacggca gtgctcgcat ctgcgaccag
     1501 cctgctctgg agaacgtaaa accttcatgg ttatccgagc catccgggaa ttcccttatt
     1561 gccaacgcga tgggaaggga gtttccatac gaggggccgt ccagggagag gtcaggattt
     1621 tggatgagga ctgtcgtgac gcgcgaatgg cgcgttcaca tccagaggga gctttcgcca
     1681 taccgacgct cgtcaccggc aggacgtggc gggccggtgc cagggatact ctgccatgag
     1741 actgcggttt caacgcggcc gcccggacaa cctcgaaaca ctccatccag cgtatttcgg
     1801 catggtcatg gcgacaggca tcgtggcaat cgccaccatg ctctgcggcg tcccgatcgt
     1861 gccaatcgtc ctgttttggc tgaacgcggc gatattcgct gtactgttgg ttgcgacagg
     1921 catgcgagcg gtgcgctatc ccggcgcgtt tttggcagac atacgcgatc acaaccgagg
     1981 tgtagggttc ttcaccctgg ttgcggcctt cgggatattc ggcaacgagc tggtaatcca
     2041 gatgcaagct gcggtcgcag ccgtgatttt ctgggccatg acggccgtac tatggttcgt
     2101 ggtgacatac ggcatcctcg cggtgcttac cgtcaagccg gacaagcccg gcctcacaca
     2161 tggcatcaac ggcggctggc tggtgatcgt ggttgccacc cagtccattt ccattctgac
     2221 agtcttgatc ctgccagcgg gtgtatcggc cgattttcac gcccggctga tgttcgcggc
     2281 gttggctctc tggctcggcg gcggggcgct ctacatgtgg cttatgacgc tgatcttttt
     2341 ccgctacacg ttccggcgga tgtccgcgga agatttgacg ccgccgtact ggatcaacat
     2401 gggcgcggtt gcaatctcca cacttgccgg cgcaacgttg ttacgcgaag cagcactttc
     2461 cccgacggtc atagagttgg cgcctttcgt gaagggcttc acactcctct tctgggcgat
     2521 tggaagttgg tggatcccga tgctcctggt tctgggggtc tggcggtata tcatgtgtgg
     2581 cgtgccattc gcttatgacc cgctctactg gggcggcgtc ttcccgctcg gcatgtactc
     2641 ggtgtgtacg cgccggttgg cggaggtgct gaacatatcg ttcctgatgc cagtgtcgac
     2701 tgcgtttgtg gtgttggcgg cattggcgtg gcttgccacc ttcgccggtc tgatcgatag
     2761 tcggttgggt gtgaagcttc gcgcatgatc gacatttcgt actagtgtcc agaggaatcg
     2821 tcggaaggga agaacgcatg agccatttct tggaacgtct gacctacctg tcccggcgac
     2881 gggaggcatt cgcggatggg cacggcgaac tgcgcgacga agaccgcacg tgggaagaag
     2941 gctatcggca gcgctggcag catgacaaga tcgtccgctc gacgcacggc gtgaactgta
     3001 ccggctcctg ctcatggaag atctacgtca agagcggcgt cgtcacatgg gagacgcagc
     3061 agaccgacta tcctcgcact cgtcccggca tgcccaatca tgaaccgcgc ggctgctcgc
     3121 gcggcgcgtc ctacagctgg tatctctaca gcgcgagccg ggtcaaatat cccatggtgc
     3181 ggggccgtct ggtgaaggcc tggcgtgagg cgcgcaagac gctcgcgcct gtagaggcct
     3241 gggcggcggt ggccggcaac gcggcgacgg caacatccta caaatcgatg cgcgggcgcg
     3301 gcggcttcgt ccggtcgacc tgggacgaag tcagcgagat cattgcggca gccaatgttc
     3361 atacgatcaa gacctggggg ccggatcgcg tggtggggtt ctcgccgata ccggcgatgt
     3421 cgatggtttc ctacgcgtcc ggcgcgcggt atctcagttt gattggcggt tccctgttgt
     3481 ctttctacga ctggtattgc gatctcccgc cgtcgagccc gcagacctgg ggtgaacaga
     3541 ccgacgtccc ggaatcggcc gactggtata attccgcgta catcatcgcc tggggctcga
     3601 acgtgccaca aacacggaca cccgatgccc acttcttcgt cgaatcccgc tacaatggta
     3661 cgaaggtcgt ggccatcacg cccgactatt cggaagtggc caagctctcc gatctttggc
     3721 ttcatccaaa gcagggaacc gatgcagctt tcgcgatggc gatgggccat gtgatcctgc
     3781 gcgagttctt cctcgatcgg caagtcccct atttccagga ttacagccgc cgctacacgg
     3841 acctgccgat gctggtgcga ttgaggcgag atggcgcgcg ctgggtgccc gaccgctatg
     3901 tgcggcaaag cgacctgcaa gactccccca agtcggaccg gg
//
LOCUS       DASSRL010000338         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1688847, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000338 DASSRL010000000
VERSION     DASSRL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688847"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..755
                     /locus_tag="VFL49_06910"
     CDS             147..755
                     /locus_tag="VFL49_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET7911742.1"
                     /translation="MISNEDHERITKAIRLAEEKTSGEIVCVLARVSSHATVFPVFAA
                     ALVALVVPWLLVAFTGMPVLRILSVQVIVFAVLLIVLCIPAVRVALLPRRARRAIAHR
                     VAMEQFINRGIARNVDRSGILIFVSLAERYARIIADDEIAKRVPQSRWQVAVDALIAH
                     AREDRIAEGFIVAIELCGQELAQHFPPTADNRGKLPDRLYLI"
     gene            complement(849..1682)
                     /locus_tag="VFL49_06915"
     CDS             complement(849..1682)
                     /locus_tag="VFL49_06915"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7911743.1"
                     /translation="METIRLGLVGDNIAASRAPDLHRAAARICGIDLTYERLVPRDLG
                     LAFDAVIERAKEQGLRGLNITHPYKERILPFVTIEDSTVRAIGACNTVLFGTQTIGLN
                     TDYTGYIAAFKERFGDTLPGMVAMAGNGGAGKAIGFALAQLGAQSLSLFDIDERKSLA
                     LADALGNQQCRMDVRVARSIQDASKNADGLINCTPRGMTGYGGNAFEGIRLNGRRWIC
                     EAVYTPIETPFLKEGRTAGVDVLSGYELFLYQGIHAFQLFTGRQVDSSTLRQMLSMAR
                     T"
     gene            complement(1692..2420)
                     /locus_tag="VFL49_06920"
     CDS             complement(1692..2420)
                     /locus_tag="VFL49_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7911744.1"
                     /translation="MRAGVQTAGEKVYHRLRSDIIFGRLAPSQKLKLETLKDAYGISI
                     STLRELLNRLTSEGLIIAESARGFEVAPVSAENFKELANLRLLLECHALQRSFAAGDM
                     DWEGRVVAAHHKLATMEKRTLAGDRRGLEILKRYDSEFHQALLSACGSRVLLDSHAAV
                     FDKYVRYLMIAVIFRDAAAPEHQKLLDCALKRDAKTAQAVLATHIMDCVSFTLANAST
                     NLLWSNRPALSGRRRVGSQATAPV"
     gene            2545..>3472
                     /gene="dctP"
                     /locus_tag="VFL49_06925"
     CDS             2545..>3472
                     /gene="dctP"
                     /locus_tag="VFL49_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET7911745.1"
                     /translation="MTKITRRSVVVGGASLLAVSAGGPALAQARQKLRFSSAFTETDL
                     RAEAYKSFAAAIKDDFDFEPYWGNTLFKQGTELVALQRDNLDLCNLAPADISKQVPAW
                     SLLTSAYLFRDADHMTKTFKSDVGREFIKMAKDQLGIQVITPVYFGSRSVNLKPDKTI
                     RTPADMAGIKLRMPPGEFWQFLGESLGANPTPVAYAELYTALQTGTVDGQDNPAVASK
                     LMKFDEVTTQFVLTRHVIAYDVMAIRSKIWDAMKPEQQAKFQAAADKAMQENTARFNK
                     QEAEVLEYFRKEGKKVYEPDQNAFRAYAQKKYV"
BASE COUNT          702 a         1024 c         1032 g          714 t
ORIGIN      
        1 tggctgtttt tcaacgtcgt gctgaatgtt ctcgccagct ccggttcgag cagaggcagc
       61 tattcgggag gcggctattc tggaggaggc ggcttttcgg gcggcggggg ctcgtcaggc
      121 ggaggcggag cgtcgggaag ctggtgatga tttcgaacga agaccacgag cgcatcacga
      181 aggctattcg gctggccgaa gagaagacct ccggcgagat cgtttgcgtg cttgcgcgcg
      241 tatcctctca tgcaacggtt tttccggtat ttgccgccgc gttggttgcg ttggtggtgc
      301 cgtggctcct cgtggccttc accggtatgc cggtcctgcg aatcctgtcc gtgcaggtga
      361 tcgtattcgc cgtgcttctc atcgttcttt gtattccggc cgtccgggtg gcgcttctgc
      421 cgcgcagggc acggcgcgcg atcgcgcacc gtgttgcaat ggagcagttc atcaatcgcg
      481 gtatcgcgcg aaacgtcgat cgttccggga tattgatctt tgtttcgctc gccgagcgat
      541 atgcacgcat cattgccgac gatgaaattg cgaagcgggt accgcaatcg agatggcagg
      601 tggcggtcga tgcgctgatc gcgcatgcgc gcgaggaccg cattgccgaa ggcttcatcg
      661 tcgcgatcga attgtgcgga caggagcttg cgcagcactt tcctccaacc gcagacaacc
      721 gcggaaagtt gccggatcgc ctctatctga tctgaaatcg ggatcgtcga agcaaccggc
      781 ggtactctct gacatcctgc cggcggcccg cgcagctgct cgcctgtttt acgcgcgccg
      841 tctgcgggtc atgtccgagc cattgaaagc atttggcgca gggtcgagga gtccacttgc
      901 cgccccgtga acaattggaa ggcgtggata ccctggtaaa ggaaaagctc gtagccgctg
      961 agaacgtcaa ctccggctgt ccgtccttct ttcaaaaacg gcgtttcaat cggcgtatag
     1021 acggcctcgc atatccagcg cctgccgttc aggcggatgc cttcgaacgc attccccccg
     1081 tatccggtca tgccgcgcgg tgtgcaattg atcaggccgt ccgcgttctt actggcgtct
     1141 tgaatcgacc gcgcaacgcg gacgtccata cggcactgtt ggtttccaag ggcatccgcc
     1201 agggcaagcg attttcgctc gtcgatatcg aataggctca gtgattgtgc gcccagttgc
     1261 gcaagcgcaa atccgatcgc tttccccgcg ccgccgtttc ctgccatggc aaccatgcca
     1321 ggcaatgtgt caccgaagcg ttctttgaaa gcggctatgt agccggtgta atcggtgttc
     1381 agaccaatcg tctgcgtgcc gaacagaacc gtgttgcaag cgccgatcgc ccggacggtt
     1441 gaatcctcga tcgtaacgaa aggcaaaatg cgctctttat acggatgcgt aatgttgagg
     1501 ccgcggagac cctgttcttt tgcccgctcg atgacggcgt cgaacgcaag gccaagatct
     1561 cgcggcacca atcgctcgta agtaaggtcg atcccgcaaa tccgggcagc tgcgcgatgc
     1621 aaatcgggcg cacgcgacgc ggcgatgtta tcgccgacca gccctaagcg gattgtctcc
     1681 aaggaccgac ctcagacagg ggctgtagcc tggcttccaa ccctccggcg cccggacaat
     1741 gccggccggt tcgaccacag caaatttgtt gacgcattgg cgagcgtaaa ggacacgcag
     1801 tccataatat gggttgcaag gacggcctga gccgtcttgg cgtcgcgctt gagcgcgcag
     1861 tccagcagtt tctgatgctc cggtgcggca gcatcccgga aaataacggc gatcatcaga
     1921 tagcggacgt atttgtcgaa gaccgcggca tgggagtcca gcaggacacg agagccgcag
     1981 gccgacagca gcgcctgatg aaattcggag tcgtatcgtt tcaggatttc caggccgcgg
     2041 cggtcgccgg ccaaagtacg tttttccatg gttgcgagct tgtgatgcgc ggcaacgaca
     2101 cgcccctccc agtccatgtc gcccgcggca aatgagcgtt gcaaggcgtg gcattcgagc
     2161 agcaagcgca ggttggcgag ttctttgaag ttttcagcgg aaacgggtgc tacctcgaag
     2221 ccgcgtgcgc tttcggcgat gatcaagccc tcggatgtca gccggttcag cagctcccga
     2281 agcgtgctga tactaattcc atacgcatct ttcagcgttt caagcttgag cttttgcgat
     2341 ggtgccagac ggccgaatat gatgtcgctg cgcagccggt gatagacctt ctctccggcc
     2401 gtctgcacgc ccgcacgcag gccactcact gaagtcccct cagatgattt gacgatcgtc
     2461 cgatgatctt gattttgact attgctgtgc ctttcgcaag ccccaaaaat gcagccagaa
     2521 cgacagcaat caggaggaat gcccatgacg aaaatcacgc gccgctccgt ggttgtcgga
     2581 ggcgcttcgc tgctcgcggt cagcgcaggc gggccggctc ttgcacaagc ccggcagaag
     2641 cttcgcttca gcagcgcttt caccgaaacc gatctgcggg ccgaggccta caaatccttc
     2701 gctgcggcga tcaaagacga cttcgacttc gagccgtatt ggggcaacac gcttttcaag
     2761 cagggcacgg agctggtcgc gctccagcgt gacaacctcg atctgtgcaa tctcgcgccg
     2821 gccgacattt cgaaacaggt gccggcatgg tcgctgctga cctcggcgta tctgttccgc
     2881 gatgccgacc atatgacaaa gacgttcaag agcgatgtcg gcagggaatt catcaagatg
     2941 gcgaaggacc agctcggtat ccaggtcatc accccggtct actttggctc ccgcagcgtc
     3001 aatctcaagc ctgacaagac gatcagaacg ccggccgaca tggccggcat caagctgcgc
     3061 atgccgccgg gagaattctg gcagtttctg ggggaatcac ttggcgccaa tcccacgccc
     3121 gtcgcctatg cggagctcta caccgcgctg cagacgggca cggttgacgg tcaggacaat
     3181 cccgcggttg ccagtaaact gatgaagttc gacgaagtca cgacccagtt cgtcctcacc
     3241 cgccatgtca tcgcttacga tgtcatggcc atccgctcga agatctggga cgcgatgaag
     3301 cccgagcaac aggccaaatt ccaggctgcc gccgacaagg ccatgcagga aaacaccgcg
     3361 cgcttcaaca agcaggaagc cgaagtgctc gaatacttca ggaaggaggg taagaaagtc
     3421 tacgagccgg accagaatgc atttcgcgcc tatgcgcaaa aaaagtacgt cg
//
LOCUS       DASSRL010000339         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1338699, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000339 DASSRL010000000
VERSION     DASSRL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338699"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFL49_06930"
     CDS             complement(<1..423)
                     /locus_tag="VFL49_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HET7911746.1"
                     /translation="MTQQAAARHDDTWKGRVEDDALLRGQGRFGDDVKPDDTLAAYFV
                     RSPHAFAKIARIDVSAATGRPGVVAVLTADDLKDAHYHSISHGHPIPGRDGKTAISPH
                     RPSLAETRVMHVGEPVVMIVAETAAAAQDAADKVVVDYE"
     gene            complement(440..964)
                     /locus_tag="VFL49_06935"
     CDS             complement(440..964)
                     /locus_tag="VFL49_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7911747.1"
                     /translation="MAKLRAAECRDFIQAVVEKAGAMGVPVSIAVVGPEGHLIAVERM
                     DEAGFITPETAIAKAFTVAAFRSMSPRFPDGLVIQQWFKERNPQLLVSLAAMTNGRIA
                     ASGGSAPVFKGDEMVGAYGISGGTSQQDEEMAIYARAKTGWAAAPEHDTTDPEVKAHI
                     NTLYAKAGITDRKL"
     gene            1077..2540
                     /locus_tag="VFL49_06940"
     CDS             1077..2540
                     /locus_tag="VFL49_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HET7911748.1"
                     /translation="MVEHTSAHAHARDWRLVMRAGGASFAVSYALLHYVVPVARWGAP
                     APVVSFFLSAIIALLAMAATLAALSRMLRTDNQRMRIAINNMSQGLCMFDGNERLVIC
                     NQRYMDMYKLSADIVKPGCTLQSLLEYRIRNGSFTRDPVEYRRDLVGAMSAGKTTSTE
                     VKSADGRSISVINRPMVDGGWVATHEDITERRDAERERVSMQEQQQRRAVIEQAIAVF
                     RQRVEDHLRTVSEGATTMHATATTLFGNSAQTSQSADGAVSASNEASTNVETAAVAAD
                     ELSGSINEIGRQLSRTTEIVRAAVTEAHGTNNQITALADAAQKIGDVIKLIRAIAGQT
                     NLLALNATIEAARAGEAGKGFAVVASEVKSLAVQTAKATEDISKLIMGVQAATTSAVS
                     AISRISGRMQEIDSCATAVSAAVEEQSAATGEISQNVASASDGAKVVVQVLGDVSGAA
                     AQTRESAENVLSASQAVDAAAAELRREVEGFLARVAA"
     gene            complement(2541..>3083)
                     /locus_tag="VFL49_06945"
     CDS             complement(2541..>3083)
                     /locus_tag="VFL49_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET7911749.1"
                     /translation="IMLASSWLLKGASARGAERTFRSLHLVSSAAYSLSHGLNDAQKT
                     MGIITVLLYSTGYLQGEFHVPHWVAISCYIAIGLGTLSGGWRIIETMGTRITKLSQHQ
                     GFSASTGGTVMLLSASWLGIPVSTTHTITGCIIGVGAAKKVSAVRWGVARGIVTAWII
                     TIPAAAAVAALFYWLSGLFG"
BASE COUNT          591 a          960 c          985 g          547 t
ORIGIN      
        1 ttcgtagtcg acaacaacct tgtcggcggc gtcctgtgcg gcggccgccg tttccgcgac
       61 gatcatgacc accggttcgc ctacgtgcat cacacgtgtt tctgcgagcg acgggcgatg
      121 cggcgagatc gcggtcttgc cgtcgcgacc cggaattggg tggccgtgcg aaatcgagtg
      181 gtagtgcgcg tccttgaggt cgtccgccgt caacaccgca acgacccccg gcctccctgt
      241 agctgcggag acatcgatgc gtgcaatctt cgcgaaggca tgcggcgagc gcacgaaata
      301 ggcggcgagc gtatcgtccg gcttgacgtc gtcgccgaaa cgtccctggc cgcgcaacaa
      361 ggcgtcgtct tcaacgcgcc ctttccaggt gtcgtcatgt cgggcggcgg cttgttgcgt
      421 catgcgatgc tccgaaatct caaagtttgc ggtcggtgat gccagccttg gcgtatagcg
      481 tgttgatatg cgctttcacc tcagggtcgg tcgtgtcgtg ttcgggagcc gcggcccagc
      541 ccgtctttgc gcgggcgtag atcgccatct cctcatcctg ctgcgacgtg ccgccggaaa
      601 tcccgtaggc gccgaccatc tcgtcgcctt tgaagacagg cgccgaaccg ccgctggccg
      661 caatgcgacc gttggtcatg gctgcaaggc tgaccagcaa ttgagggttg cgctccttga
      721 accactgctg gatcaccagg ccatcaggga agcgcgggct catcgaacgg aatgcggcaa
      781 ccgtgaaagc cttggcgatt gcggtttccg gggtgatgaa cccggcctcg tccatccgct
      841 caacagcgat gaggtgtccc tccggtccca ccaccgcgat cgaaacgggc acgcccatgg
      901 cgccggcttt ttcgaccacg gcctggatga aatcgcggca ttccgccgcc cgcaacttgg
      961 ccatcctccc aaccctccag cttggcctgt taagctttgc ttaacctgtt atgtccggca
     1021 attaactgca aaaaaacggg ttggggaata gcgtccgggc ttacctggac gcgaacatgg
     1081 ttgagcatac ctccgcacac gcccacgcgc gcgactggcg tttggtcatg cgggccggcg
     1141 gagcaagctt tgccgtcagc tatgccctgc ttcattacgt cgttcccgtc gccaggtggg
     1201 gtgccccggc gcccgtcgtg tcctttttcc tctccgcaat catagccctt cttgccatgg
     1261 cagcgacctt ggcagcgctc tcccgaatgc tgcgcacgga taaccagcgc atgcggattg
     1321 cgatcaacaa catgtcgcaa ggcctgtgca tgttcgacgg caacgaacgg ctggtgatct
     1381 gcaatcaacg ctacatggat atgtacaagc tgtcggccga tatcgttaag ccgggctgca
     1441 cgctgcaaag ccttctcgag tacagaatcc ggaacggcag ctttacgcgt gaccccgttg
     1501 aataccggcg cgaccttgtt ggcgccatgt ccgcgggcaa gacgaccagc accgaagtga
     1561 agtcggccga tggacggtca atttcagtca tcaaccggcc catggtcgat ggcggctggg
     1621 ttgcaacgca tgaggacatc accgagcggc gcgatgccga gcgcgagaga gtctcgatgc
     1681 aggagcagca gcagcggcgc gccgtgatcg agcaggcgat cgctgtcttc cggcaacgcg
     1741 tcgaggatca tctcaggacg gtcagcgaag gtgcgacgac gatgcatgcg accgccacga
     1801 cattgttcgg caattcggca cagacgtcgc agagtgccga tggcgcggta tcggcctcga
     1861 acgaggcatc gacgaacgtg gaaacggccg cggtcgcggc ggacgagctg agcggatcga
     1921 tcaatgaaat cggccggcag ctctccagga caaccgaaat tgtgcgggca gctgtgaccg
     1981 aggcacacgg aaccaataac cagattaccg cgctcgcaga cgccgcgcag aagatcggcg
     2041 acgtcatcaa gctgatccgc gccatcgccg gacagaccaa tctgcttgcg ctcaacgcca
     2101 cgatcgaagc ggcgcgcgcc ggcgaggccg gcaagggctt cgcggtggtt gcttccgaag
     2161 tcaagtcgct cgcggtgcag accgccaagg cgacggagga tatctccaag ctgatcatgg
     2221 gcgtgcaggc ggcaaccacg agcgccgttt cggcgatcag cagaatctcc gggcgcatgc
     2281 aggagatcga cagttgcgcg actgccgtgt ccgctgcggt cgaagagcag agcgccgcga
     2341 ccggagaaat ttcacagaac gtcgccagcg cgtccgatgg cgcaaaagtc gtggtccagg
     2401 tactgggcga cgtgtccggg gcggcggcac agacgcggga atcggccgaa aacgtgctct
     2461 cggcttcgca ggcggtggac gcagccgcgg ccgaattgcg cagggaagtc gaaggcttcc
     2521 tggcgcgtgt cgccgcctga tcagccgaaa agaccgctca gccagtaaaa cagcgcggca
     2581 actgccgccg ccgccggaat cgtgatgatc caggcggtga caatccccct ggccacgccc
     2641 cagcgtaccg ccgatacttt ctttgcggct ccgacgccga tgatgcagcc ggtgatggtg
     2701 tgcgtggtcg aaacagggat gccgagccat gaggcggaaa gcagcatcac ggtgcccccg
     2761 gtcgaagcgc tgaagccctg gtgctgcgac agcttggtga ttcgcgtgcc catcgtctcg
     2821 atgatccgcc agccgcccga gagagtgccg aggccgatcg cgatgtaaca gctgatcgcc
     2881 acccagtgcg gaacgtggaa ctcaccttgc agatagccgg tcgagtagag cagcacggtg
     2941 atgatgccca tcgtcttctg ggcgtcgttg agcccgtggc tgagtgaata ggcggccgag
     3001 gataccagat gcagcgatcg gaacgtccgc tcggcgcccc ttgccgaggc acccttcagc
     3061 agccagctgg aggcgagcat gat
//
LOCUS       DASSRL010000340         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_26184, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000340 DASSRL010000000
VERSION     DASSRL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26184"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFL49_06950"
     CDS             complement(<1..343)
                     /locus_tag="VFL49_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET7911750.1"
                     /translation="MTDPQTAIPSRAPSATTADDTVMPFEVAKLDLRGRVVRLGPLVD
                     EILSRHAYPPPVAKLLGEAIVLTVMLGSAVKIDGRFILQTQSDGPVRMLVVDFVAPDK
                     VRACARFDKNRV"
     gene            complement(409..1335)
                     /gene="argF"
                     /locus_tag="VFL49_06955"
     CDS             complement(409..1335)
                     /gene="argF"
                     /locus_tag="VFL49_06955"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7911751.1"
                     /translation="MTVNGVRHFLDLTDIPKPVLAGLIDRSRAMKAARANGQLASPLA
                     GKTLAMVFDKPSTRTRVSFDVAMRQLGGEAILLTAQEMQLGRGETLADTARVLSRYVD
                     AIMIRILDHEAVHELARHATIPVINGLTRRSHPCQVLADVMTYEEKRGPIRGRTVAWS
                     GDANNVLASWMHAAERFEFQLRVATPTELRPKKWLMDWVKSSGAAIEIGDDPDAAVKD
                     ANCVVTDTWVSMGDRDGKLRHNILKRYQVNGRLMAKARPDALFMHCLPAHRGEEVTDE
                     VIDGPQSVVFDEAENRLHAQKGLLAWCLHVDA"
     gene            complement(1332..2534)
                     /locus_tag="VFL49_06960"
     CDS             complement(1332..2534)
                     /locus_tag="VFL49_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7911752.1"
                     /translation="MTTHLLPTYARVDLAFERGEGAWLFTAEGDRYLDFTAGVAVNAL
                     GHAHPHLVKTLTDQAHKVWHVSNLYRIPEGERLADRLCAITFADTVFFQNSGAEAIEC
                     AIKMVRKYQSVSGKPERYRIITFEGAFHGRTLAAIAATGNKKYLEGFGPPVEGFDQVP
                     FGDLEAVKCAIGPETAAILIEPIQGEGGVRVASPTFLRALRQLCDQHGLLLVFDEIQT
                     GFVRSGEMFAYERSGVKPDIMTIAKALGGGFPVGACLATTEASKGMTVGTHGTTFGGN
                     PLAMAVGNAVLDIMTDPEFLEQVRKNALLLKQRLAELKDRYASVIAEVRGEGLLIGLR
                     MIPQASEMVDELRAEKMLTVGAGDNVVRLLPPLIIGDEEISEAVTRIDRACARLAQTH
                     ARTAKVAS"
BASE COUNT          531 a          928 c          887 g          580 t
ORIGIN      
        1 ccacccggtt tttgtcgaag cgcgcgcagg cgcgcacctt atccggcgcg acaaagtcga
       61 ccaccagcat gcgcaccggg ccgtcgctct gcgtctgcag gatgaaacgg ccgtcgattt
      121 tcaccgccga gccgagcatc accgtcaata cgatagcctc ccccaggagc tttgccacag
      181 gcgggggata ggcatggcgc gaaaggattt cgtccacgag cggcccgagc cgaacgaccc
      241 ggccgcgcag gtcgagcttg gccacctcga agggcatgac ggtatcgtca gcggtggtcg
      301 cggacggcgc gcgggatgga atagccgttt gaggatcggt catgcgccct atgtagcgtt
      361 caacagccga aatgcgaggg cttgttccga agccggctat gcgcggcctc acgcgtcgac
      421 gtgcagacac caggccagaa ggcccttctg cgcatgcaga cggttctcgg cttcgtcaaa
      481 gaccactgac tgcgggccgt cgatgacctc atcggtcacc tcttcgccgc gatgcgccgg
      541 caggcagtgc atgaacagcg catccggcct ggctttggcc atcaagcggc cattgacctg
      601 gtaccgcttg agaatgttgt gccgaagctt gccgtcccgg tcgcccatgg acacccatgt
      661 gtcggtgacc acgcagttgg cgtccttgac cgcggcatcc gggtcatcgc cgatctcgat
      721 cgcagcgcct gacgatttta cccagtccat cagccatttt ttcggccgca actccgtcgg
      781 cgtcgcgacc cgcaactgga actcgaaccg ctcggcggcg tgcatccagc ttgccagcac
      841 gttgttggcg tcgccgctcc aggcaacggt gcggccacgg atcggacctc gcttctcttc
      901 gtaggtcatg acgtcggcca gcacctggca aggatgagaa cggcgcgtca atccgttgat
      961 gaccgggatg gttgcatgcc gcgcgagctc atggaccgcc tcatgatcga ggatccggat
     1021 catgatggcg tcgacataac gcgacaggac ccgcgccgta tcggcgagcg tttcgccgcg
     1081 cccaagttgc atttcctggg ccgtgagcaa aattgcctcg ccccccagct ggcgcattgc
     1141 gacgtcgaac gagacgcgtg tgcgggtcga cggcttgtcg aacaccatcg ccaacgtctt
     1201 gccggcaagc ggagacgcaa gctgaccgtt cgcgcgcgcg gccttcatcg cgcggctgcg
     1261 gtcgatcagc ccggcgagaa ccggcttcgg aatgtcggtg agatcgagaa aatgccggac
     1321 gccgttcacg gtcatgacgc caccttcgcc gtccgcgcgt gggtctgcgc cagccgcgca
     1381 caggcgcgat cgatccgggt gacggcttcc gatatttcct catcgccgat gatcagtggc
     1441 ggcagaaggc gtacgacgtt gtcgccggcg ccgaccgtga gcattttttc cgcgcgcagt
     1501 tcatcgacca tttcggacgc ttgcgggatc atgcgcaggc cgatcagcag cccttcgcca
     1561 cgcacctccg cgatcacgga cgcatagcgg tctttcagtt cggcgagccg ctgcttgagc
     1621 aacagtgcat tttttcgcac ctgctcgaga aattcgggat cggtcatgat gtccagcacg
     1681 gcattgccga ccgccatggc gagcggatta ccgccgaagg tcgttccgtg cgtcccgacc
     1741 gtcatgcctt tcgaggcttc cgtcgttgca aggcacgcgc cgaccggaaa gccgccgcca
     1801 agcgccttcg caatcgtcat gatgtccggc ttaacgccgc tgcgttcata agcgaacatt
     1861 tcacctgaac ggacaaaacc ggtctgtatt tcgtcaaata ccagcaagag cccgtgctgg
     1921 tcgcaaagtt ggcgcagcgc gcgcaggaat gtcggcgatg cgacccgcac gccgccttcg
     1981 ccttgaatcg gctcgataag tatcgcagcc gtctccggcc cgatcgcaca cttgaccgct
     2041 tcaaggtcgc cgaacggcac ctgatcgaag ccttcgaccg gaggtccaaa gccttccaga
     2101 tatttcttgt taccggttgc agcgatcgca gccaacgtgc gcccatggaa ggcgccctca
     2161 aaggtgatga tacgatagcg ttccggtttt ccggagaccg actgatactt gcgtaccatc
     2221 ttgattgcgc actcgatcgc ctcggcgccg gagttctgga agaacacggt gtcggcaaag
     2281 gttatcgcac atagcctgtc ggcaagcctt tcgccttccg ggatgcggta aagattggag
     2341 acgtgccaga ccttgtgcgc ctggtcggta agcgttttga ccaggtgcgg gtgggcatgg
     2401 ccaagcgcat tgaccgcgac gcccgcggtg aaatccaggt acctgtctcc ttcagccgtg
     2461 aacagccatg cgccctcgcc ccgctcgaac gcgaggtcga cgcgcgcata ggtcggcaat
     2521 agatgcgtgg tcacggagtt tctttcgtca acttggcggc ttgcgcatgg gcttgtccga
     2581 agcgtatccg cttttcggga ccacgcgcgc gccgaaaaga aatgtgccgc ctcgccgggc
     2641 ggcacttttt tcattctatt gccggacctt gctctgtcaa cacagcggcc gctctttgaa
     2701 tctccgactc aattgcgccg caaccccaga ttctaaggga aaaactgttg gtagctgcac
     2761 ggagagcgct gtgagtgctt gcacgggagt cactgcctat tgtagcgttt cggtcaggta
     2821 gtacgacatc tcgtgctacg gggcttcttg ttcattcctg acgcgtacgc gtctggcgct
     2881 gatgtgcgcc cgacgacggg gtcggattcc atccaaggcc ttgggg
//
LOCUS       DASSRL010000341         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1076447, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000341 DASSRL010000000
VERSION     DASSRL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076447"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1464)
                     /locus_tag="VFL49_06965"
     CDS             complement(208..1464)
                     /locus_tag="VFL49_06965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET7911753.1"
                     /translation="MRLLSNLLRRFIRKGTLRVRDADGKVHVFGGLLPGPDVAIHLHD
                     RKLYTKLFVNPELHTAEAYMDGTLTLEDGAQIHDFLLLFSVNRAALYSYGSQKLMRRA
                     WRSLRRWHQANPIGLAAAHARHHYDIPIELYRLFLDDQMQYSCAYFSDPEHDTLEQAQ
                     RSKLIHATSKLQLKPGMTVAEIGSGWGGFAIHLARETGARVTGVNVSPEQIKIARAKA
                     EAAGVSDRVEFRELDYRQFTGQFDRVVSVGMMEHVGAGHYDEYFQKIRELLTPDGYAF
                     IHCIGRMSPPGATGPFIRKYIFPGGYVPALSETFAATERWGLWCNDMEVLRLHYRYTV
                     KHWRERFAKNRAQAAAICDERFCRMWEFYLAAVELEFLHGSHMVFQLLLSTKRDAVPI
                     TRDFMIDAERGAFAKTAQSAPSGRLS"
     gene            1682..1939
                     /locus_tag="VFL49_06970"
     CDS             1682..1939
                     /locus_tag="VFL49_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET7911754.1"
                     /translation="MASAADDIIEMITDRISATSGALQLSEPLDKLGLDSMQVVELIF
                     DIEEKFDINLPVNANMDIESKTVGDLIQAVDQLVAAKAKPN"
     gene            1936..>2414
                     /locus_tag="VFL49_06975"
     CDS             1936..>2414
                     /locus_tag="VFL49_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7911755.1"
                     /translation="MNRVVITGFGVVSSVGQSTRDYWDSLIAGRSAFAPPTLGTAAQA
                     SGKLVGEVKDFDPAAHFESSQVPLLDRVSQFAIVAARQAVSQSGLELRGEIAERTAAI
                     IGTGSGGHTTIDDSFRRLYSGGAARLHPLTIPKAMISAPASQVSMYCGIKGPVFSVSS
                     "
BASE COUNT          522 a          686 c          719 g          487 t
ORIGIN      
        1 gccgcataag ggcatctgat ttgacgccca gcctcgcgac gatgcctccc tgggatccgt
       61 cggcctgaga cgagcgtgat cctggcctga cgccgccgac gccctgcgag cttagtttcg
      121 cgggaatcca taaccggtgg ccagccgctg tgcaaaggac gactggctct gccaaagcca
      181 aatcaacacc atccaaatca acgcaatcta tgacaaacgg ccgctagggg cgctttgcgc
      241 cgtcttcgca aacgcgcccc gctcggcgtc gatcatgaaa tcgcgtgtga tcggcaccgc
      301 atcgcgcttg gtcgaaagca gcagttgaaa caccatgtgc gagccgtgaa ggaattcgag
      361 ttcgacggca gcaagataga attcccacat ccggcagaat ctttcatcgc agatcgccgc
      421 tgcctgggct cgatttttgg cgaaacgctc gcgccaatgc ttgactgtgt aacgataatg
      481 cagccgcaga acttccatat cgttgcacca caggccccag cgctcggtgg cagcgaaagt
      541 ttccgacagc gcgggcacat acccgccggg aaaaatgtat ttgcggatga acgggccggt
      601 cgcgcccggc ggcgacatcc ggccgatgca atggatgaaa gcatagccgt caggcgtcag
      661 taactcgcgg atcttctgga aatactcgtc gtaatggccc gcgccgacat gttccatcat
      721 gccaaccgag acaacacggt caaactggcc ggtgaactgc cgatagtcca gctcacgaaa
      781 ctcgacacgg tcggaaacgc cggcagcttc cgccttggcg cgcgctatct tgatctgctc
      841 gggcgaaacg ttgaccccgg tgacacgggc gcccgtttcg cgcgcaagat ggatggcgaa
      901 gccgccccag ccagagccga tctcggcgac ggtcatgccg ggctttagtt gcaacttgct
      961 ggtggcgtgg atcagcttgc tgcgctgggc ctgctcaagc gtatcgtgct cgggatcgct
     1021 aaagtaagcg caggaatatt gcatctgatc gtcgaggaac aggcggtaaa gctcgatcgg
     1081 gatgtcgtaa tggtgacggg catgagcggc ggcaaggccg atcgggttgg cctgatgcca
     1141 gcggcgcaga ctgcgccacg cccgccgcat caatttctgg gagccgtagg aatagagcgc
     1201 agcgcggttg accgagaaca gcagaagaaa atcgtggatt tgagcgccat cctcgagagt
     1261 cagcgtgccg tccatatagg cttcggctgt atgtagttcc ggattgacga acaacttggt
     1321 ataaagcttg cgatcatgca gatggatggc gacgtcgggt ccgggcagga gaccgccgaa
     1381 tacatgcact ttcccgtcgg cgtcgcggac ccgcaatgtg cccttgcgga tgaagcgccg
     1441 aagcagattg gacaatagac gcacctttgt cgtcctccgc ggtcgtccca aatcttcata
     1501 gcaatgtatc cggaagagat cacgtggccg gaagacgcgg cgcagaatta cctcatggaa
     1561 tatctgcggt aaatccgcta tctccggtaa acgctgcccg gaccgtcttt agctttcggg
     1621 caatcctgcc gtctgctatc gtttgcgcca cccggaaatg tctgaaggat aaagtgcagt
     1681 tatggcgagt gctgctgatg atattatcga gatgattacg gatagaatca gcgccaccag
     1741 cggtgcgctt cagctgtcgg aaccgctcga caagctggga ctggactcca tgcaggtggt
     1801 tgagttgatt tttgatatcg aggaaaaatt cgacataaac cttccggtca acgcgaatat
     1861 ggacattgaa agcaagacag tgggggatct cattcaagcg gtggaccagc ttgttgccgc
     1921 taaagcgaaa ccgaattgaa ccgggttgtt attaccggct tcggtgttgt ctcatccgtt
     1981 ggacaatcga cgcgggacta ttgggattcg ttgatcgcgg gacgctcggc tttcgcgccg
     2041 ccaacgctgg gaaccgctgc gcaggccagc ggcaagctcg tcggtgaagt caaagatttc
     2101 gatccggcgg cccattttga atcatcgcag gtcccgctgc tcgaccgggt ttcgcaattc
     2161 gccatcgttg ccgcgcggca ggcggtgagc caatcgggac ttgagttacg cggcgagatt
     2221 gccgagcgaa ctgccgcgat catcggtaca ggatcgggag gacatacgac tatcgatgac
     2281 agcttccgcc gtctctattc gggcggcgcc gcgcgccttc atcccctgac cattcccaag
     2341 gccatgatca gcgcgccggc cagccaggta tcgatgtact gcggcatcaa aggtccggta
     2401 ttttcggttt catc
//
LOCUS       DASSRL010000342         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_44033, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000342 DASSRL010000000
VERSION     DASSRL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44033"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFL49_06980"
     CDS             <1..195
                     /locus_tag="VFL49_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911756.1"
                     /translation="FSGVPKIEIDLIDRPTEKPVGAGEAACTAVGASLANAVHNATGA
                     RLRTVPFTPERVKLALTGKA"
     gene            296..1423
                     /gene="proB"
                     /locus_tag="VFL49_06985"
     CDS             296..1423
                     /gene="proB"
                     /locus_tag="VFL49_06985"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET7911757.1"
                     /translation="MPKAPLLKDFHRIVVKVGSSLLVDSSAGGLKRDWLASLAADIAG
                     LHRKKCDLLVVSSGAIALGRAVLKLPPGALKLEDSQAAAAVGQIALARAWSEALGAHS
                     LTAGQVLVTLGDTEERRRYLNARSTIAKLLEWRSVPVINENDTVATTEIRYGDNDRLA
                     ARVATMMSADLLVLLSDIDGLYDRPPMAGNGARLVPVVTRITPDIEAMAGASGSELAR
                     GGMLTKIEAGKIATTAGTHMVIASGRVLHPLQAIANAAACTWFLTPANPVTARKKWIA
                     GSLEPRGALLIDAGAVRALRSGKSLLPAGVIKVEGGFGRGDAVVVRGPDGQEVGRGLV
                     AYDAEDAEKIKGCSSANVLLILGYTGRTEMIHRDDLVLGGE"
     gene            2054..>3265
                     /locus_tag="VFL49_06990"
     CDS             2054..>3265
                     /locus_tag="VFL49_06990"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET7911758.1"
                     /translation="MTAPLKSIEGSGDIAAAMGEIGCRARAAARALALAPSAQKDKAL
                     GSAAAAIRSQTSLILAANAEDLAEARAAGVAGAFLDRLALDQARVEAMAAGVEAVRTL
                     NDPVGAVTESWTRPNGMRIERVRVPLGVIGIIYESRPNVTADAGALCLKSGNAAILRG
                     GSDSMRSSRAILAALAEGLRAADLPEAAIQLVPTRDRAAVGLMLAGLDGNIDVIVPRG
                     GKSLVARVQAETRVPVFAHLEGVCHVYVDKAASLEMAKKIVLNAKMRRTGVCGAAETL
                     LVDRACAGTHLKPLIEMLIAAGCEIHGDKAALAVDNRVKPATEEDWGTEYLDAIIAVK
                     VVDSVEAAIDHIERYGSRHTDAIITEDHPTAEKFLREVDSAIVLHNASTQFADGGEFG
                     FGAEIGIATGRL"
BASE COUNT          594 a         1066 c         1053 g          552 t
ORIGIN      
        1 ttctccggcg tgccgaagat cgagatcgat ctgatcgacc ggccgacgga aaagccggtt
       61 ggtgccggcg aggcggcttg cacagccgtc ggcgcatcac tcgccaacgc cgtccacaac
      121 gccaccggcg cacgcttgag gaccgttcct ttcaccccgg agcgggtgaa actggcgtta
      181 accgggaaag cataaaggct gccggattcg attgatccgg cggcgactca atcctgacgg
      241 accattttcg acgtgctaga caaccggcgc gatcaatgac gccgttgccg aagccatgcc
      301 gaaagcaccg ctgctcaagg acttccaccg catcgtggtg aaggtcggtt cttcgttgct
      361 ggtggattcc tctgccggcg gcctcaagcg cgactggctc gcttcactcg cggccgacat
      421 cgccggcctg catcggaaaa aatgcgatct gctcgtggtc tcgtcgggcg ccatcgcact
      481 cggccgcgcc gttctgaagc tgccgccagg cgcgctcaag ctcgaggaca gtcaggcggc
      541 ggccgccgtc gggcaaattg cccttgcgcg cgcgtggtcg gaagcgcttg gcgcgcattc
      601 gctgaccgct ggccaggtgc ttgtcacgct tggcgatacc gaagaacgcc ggcgctacct
      661 caatgcgcgt tcgacgatcg cgaagctgct cgaatggcgg agcgtgccgg tcatcaatga
      721 aaacgacacc gtggccacga cggaaatcag gtatggcgac aacgaccgcc tcgctgcgcg
      781 agtcgccacc atgatgagcg ccgacctgct cgttctgctt tccgacatcg atggtcttta
      841 tgaccggccg ccgatggccg ggaacggcgc cagactggtg ccggtcgtca cgcgcatcac
      901 gccggacata gaagcgatgg ctggggcatc cgggtccgag ctcgcacgcg gcggcatgct
      961 gaccaagatc gaagcgggta aaatcgccac cacggccggg acccatatgg tgatcgcctc
     1021 gggccgcgtg ctgcatcccc tgcaggcgat cgcaaatgca gcggcctgta cctggttcct
     1081 gacgccggcg aatcccgtga ccgcgcgcaa aaaatggatc gcaggatcgc tcgagccgcg
     1141 cggtgccctg ttgatcgatg ccggggccgt gcgtgcgctg agaagcggaa agagcctgct
     1201 gccggcgggc gtcatcaagg tcgagggcgg cttcgggcgc ggtgatgccg tcgtggtgcg
     1261 ggggccggac ggccaggagg tcgggcgcgg actcgtcgcc tatgacgccg aagacgccga
     1321 aaagatcaaa ggctgctcgt ctgccaatgt gcttctgatc cttggctaca cgggccggac
     1381 cgaaatgatc caccgcgacg atctggtgct cgggggcgaa tagccgacag cgcggaaccg
     1441 ctccgcgtcg cagcgcgtta cctgtctgga cacacgtgag aaaggcgatt gaccgatcgc
     1501 ccgtcagccc cagcccaccg gtggctgggg ttttcgcgtc taccgggcca ttggccgagt
     1561 ggccgttcgc ttcgccccgg cagccgtccg ccaacggacc ctccgccggt tgcgtcggcc
     1621 attggccgat tccactgggt cagtgggccc ggcgattcga gcgccgccgc gcacgccgca
     1681 ctggcgcaat ggtcttttgc tttgccggcc gggccggtaa cggcaggaac gtgccgtcag
     1741 gaaattccag atgccggctg aacacttgca cttggccttc cccgcggcgc tttccttcga
     1801 tcacaaactt ggtcccgaca ggaaaacggc tgggaagacg gcttgagcta tacgccatga
     1861 ctacctcctg ggatgccgcc accctgccac tgcaatgtgg aggtagttaa accgttgcat
     1921 ggcaccacct gttgtccacg ctatccccag gctcgaacct gctatgcgtt cagccggccg
     1981 acctcgccgc cgggcacccg acgtgatagg aagtgcccca ggacaagggg ttttcaccgg
     2041 ctttctgcga aaaatgaccg ccccgctcaa aagcatcgag ggatccggcg atatcgccgc
     2101 cgccatgggc gaaattggct gccgcgcccg ggcagctgcc cgcgcgctcg cccttgcccc
     2161 aagcgcgcag aaggataaag ccctcggctc tgcggcggcg gcaatacggt cacaaacctc
     2221 gctcatcctc gccgcgaatg ccgaagattt ggccgaagcg cgcgccgccg gggtcgcagg
     2281 cgcttttctc gaccgcctgg cgctggatca ggcgcgcgtc gaagcgatgg cggccggcgt
     2341 cgaagcagtg cggaccctga acgatcccgt cggcgctgtg acggagtcat ggactcggcc
     2401 gaatgggatg cgaatcgaac gggtccgcgt ccctctcggg gtcatcggca tcatttatga
     2461 gagccgcccc aatgtgacag ccgatgccgg ggccttgtgc ctgaaatccg gcaacgcagc
     2521 gatcctgcgt ggcggctcgg acagtatgcg ctcgagccgg gcgattttgg cagcgctggc
     2581 cgaaggcctg cgcgcggccg acctgcctga agctgccatc cagctggtac cgacccgtga
     2641 ccgcgcggcc gtcggcctca tgctggccgg tctcgacggc aatatcgatg tgatcgtccc
     2701 gcgaggcggc aaaagtctcg tggcccgcgt gcaagccgag acacgcgtgc cggtatttgc
     2761 acacctcgaa ggtgtctgcc acgtctatgt cgacaaggcg gcctcgctcg aaatggccaa
     2821 gaagatcgtt ctcaatgcga aaatgcggcg cacgggcgtt tgcggcgcag ccgaaacgct
     2881 gctcgtcgac cgcgcctgcg ccggcaccca tctcaagccg cttattgaga tgctgatcgc
     2941 cgccgggtgc gaaatccacg gcgacaaggc ggcgctagcg gtcgacaatc gggtcaagcc
     3001 cgcaactgaa gaagactggg gcaccgagta tctcgatgcc atcatcgcgg tcaaagtcgt
     3061 cgacagtgtc gaggccgcga tcgaccacat cgaacgctat gggtcgcgcc acacggacgc
     3121 gatcattacc gaggatcacc caacggccga aaagtttctg cgcgaagtcg attccgccat
     3181 cgtcctgcac aacgcatcga cgcaatttgc cgatggcgga gaattcggat tcggcgccga
     3241 gatcgggatc gccaccgggc gactg
//
LOCUS       DASSRL010000343         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_954021, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000343 DASSRL010000000
VERSION     DASSRL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954021"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1168
                     /locus_tag="VFL49_06995"
     CDS             224..1168
                     /locus_tag="VFL49_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911759.1"
                     /translation="MMPSRVIAGCLAGSLLAAGPVLAQDSKRPDKVSFYFAAHEDDWQ
                     LFMNPSAFEDVIRGAAKTVFIHLTAGDAGLGIGMGGRKHPFYLARENGAEAAIRFMAN
                     ADAAPAERLAAPMPFNGHSIYRVSYHNTVGYFLRVPDGHWSGDGYYETGFESLKRLAS
                     GENNTLHAVDGSTTYHGWNDLVATLRAIVAYERGKAAVIQVNVAETDARINPQDHSDH
                     LMTAKAALDAVKDSTCVRRVYYVDYASSRLPQNLDVQKRDMESSVLAVTLAGVQALDH
                     STAWHHYDESFVGRNYFRVEAPAGHCDAPLAALAARPR"
     gene            1320..>2115
                     /locus_tag="VFL49_07000"
     CDS             1320..>2115
                     /locus_tag="VFL49_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HET7911760.1"
                     /translation="MRYADIPLALQTRRVLSVDALRGFSIFWIIGADGAVIALERILR
                     DKGPVLASIGSFLGTQMTHADWEGFRFYDFIFPLFIFITGASIVLALPRLVEREGRAK
                     VHLHVLRRALVLYVLGVIYYGGVGHQLSDMRFVGILQRIAVCYLFASILFLNLSWRGL
                     VAALVVLLVGYWALLTFVPVPGVGVASYQPETNLANWIDLHFLPGRLWDETRDPEGLL
                     STIPAIGTCLLGVLAGLLLKDERLSPEQKTLWLIAAGVAMIAAGYLW"
BASE COUNT          381 a          622 c          642 g          470 t
ORIGIN      
        1 tggcggacga agggcgcggg tttcatgggg tagactttcc agcgcgacca ggactattta
       61 tttgtatgca aacgataaag cgccgtcccg gattgtcaaa cgaaggccga aaagcggctg
      121 cgacagtgtg cgcaagacgg ctgttttttc ttagaaagcg ccaaattcgt attctatctg
      181 tcagtgcgcc ggggcgttgc aagacgaggg gcggggaatg ctgatgatgc caagtagagt
      241 cattgcgggc tgccttgccg gtagtctgct ggcagccgga ccggtgctgg cccaggacag
      301 caagcgtcct gacaaggtct cattctattt cgccgcccac gaggacgact ggcaactgtt
      361 catgaatccg tccgccttcg aggacgtgat caggggcgcg gcgaaaaccg tgttcatcca
      421 tctcactgcc ggcgacgcag ggctcggcat cgggatgggg ggccgcaagc atccatttta
      481 tcttgcgcgc gaaaatggag ccgaagccgc tatccggttc atggccaatg ctgatgccgc
      541 gcccgccgag cgccttgcgg cgccgatgcc gttcaacgga cattcaatct atcgcgtgag
      601 ctatcacaat actgtcggct attttctgcg cgtcccggac ggacactggt ccggtgacgg
      661 ctactacgaa accgggtttg aatcgctcaa gcggctggcg agcggcgaga acaatactct
      721 gcatgcggtg gacggatcga cgacctatca cggttggaac gatctggtgg cgacgttgcg
      781 ggcgatcgtc gcttacgaac gcgggaaggc ggccgttatt caggtcaatg ttgccgaaac
      841 cgatgcacgc atcaatccgc aggatcactc cgaccatctc atgacggcga aagccgcgct
      901 cgatgccgtt aaggattcga cctgcgtacg gcgcgtctat tacgtcgact acgccagttc
      961 acgtctgccg caaaacctcg acgtgcagaa gcgggacatg gaaagctcgg ttctcgccgt
     1021 gactcttgcc ggtgttcagg ctctcgacca cagcacggcg tggcaccact atgacgaatc
     1081 ctttgtcggc cgaaattact tccgcgtcga ggctccggca gggcactgcg atgcaccgct
     1141 cgcagcgctg gcggcgaggc cgcggtaggt cgcccacagt gccaggcttc acgatcgggt
     1201 gatcgccagc tcatgggtga tgtgagcccg ccgccaggcg gctatttttc cgcaaccaat
     1261 tccgcgttga cccgttttcg cttggtcaaa gcctgcctcg gaccgtactg gcggtgtcca
     1321 tgcgctacgc agatataccg ctcgcattac agacgcgtcg cgttctgtca gttgatgccc
     1381 tgcgcgggtt cagcatcttc tggatcatcg gtgccgacgg cgccgtcatc gctctcgaac
     1441 gcatcctgcg cgacaaaggt ccagtgctcg cctcaatagg gagttttctc ggcacccaga
     1501 tgacccacgc ggactgggaa ggattccgat tctacgattt catcttccca ctgttcattt
     1561 tcatcaccgg ggcttccatc gtgcttgctc tgccgcgcct ggtcgagcgc gagggccggg
     1621 ccaaagttca cctgcatgtg ctgcgccgcg cgcttgtctt gtatgtgctt ggagtcattt
     1681 actacggcgg tgtcggccat cagttgagcg acatgcgctt tgtcggcatc ttgcagcgaa
     1741 tcgcggtctg ctacttgttc gcgtcgatcc tgttcctcaa tctgagctgg cgcgggctgg
     1801 ttgccgcgct tgtcgtgttg ctggtcggct attgggcgct gctgaccttt gtgccggtgc
     1861 cgggtgtcgg cgtcgcctcc taccagcctg aaaccaacct cgccaactgg atcgatctgc
     1921 attttctgcc ggggcgattg tgggacgaga cacgcgaccc ggagggattg ttgagcacca
     1981 tcccggccat cggcacatgc ttgctcggcg tgttggccgg ccttttactt aaagacgaac
     2041 gattgtcccc ggaacagaaa actttatggc tcatcgcagc cggcgttgcc atgattgccg
     2101 ctggctatct gtggg
//
LOCUS       DASSRL010000344         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1198973, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000344 DASSRL010000000
VERSION     DASSRL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198973"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFL49_07005"
     CDS             <1..254
                     /locus_tag="VFL49_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911761.1"
                     /translation="ETSGTTAILVTHSIEEAILLADTILVMTARPGRIAARIEVELPR
                     PRSVATTRDPGFSALFDRIYGLLRDEVMRAMVSGQGDGQ"
     gene            251..1063
                     /locus_tag="VFL49_07010"
     CDS             251..1063
                     /locus_tag="VFL49_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7911762.1"
                     /translation="MSISPLAKDDAPARTKSGYAPLDDPRVLGWGSIVLVLVVWELTA
                     RLFNVSALYLPRPSQIVVALITLFGTGGLAYDLGVTLWRIFAGFAIALVSGTLLGLWI
                     ATSFRARAVADMFIAALYPLPKITLIPLLIIWLGTGGPFMLTISFLGAIFPIVINTVL
                     GVSQCDQGLVLAARDLGATPPQIMRRVLLPSAIPSIFAGVRLGLGVSIILVVAAEMVV
                     GKAGLGARLYLAGQILETEQVFAILIVLAMLGIIVTKVQDAVDAVLGNWRVD"
     gene            1106..1564
                     /locus_tag="VFL49_07015"
     CDS             1106..1564
                     /locus_tag="VFL49_07015"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7911763.1"
                     /translation="MTVMTKPDKTFRLDPVHPKPEEAHTMPYAPAVHIVGACDLMFVS
                     GATSSPLYHKHPHVDEEHVQPHAIEEQTERAMASIKSILDHVGASWRDVVKITKYLTD
                     FRDADAMHRTMGKYFGDWRPASTTVCINQLSSPGARVELDMIVALPKKAT"
     gene            1561..>2248
                     /locus_tag="VFL49_07020"
     CDS             1561..>2248
                     /locus_tag="VFL49_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911764.1"
                     /translation="MTLPPHLARIVEAEYPRFSEQEMTRRRAAVEKLLEQAGCDHLVF
                     CGANRLGSAVQWLTQWPVTAEAVGVLTPRERDALFVQYVNHAPQAGILADKADVAWGG
                     DSSIAAAIKTLERRGARVDRVATLGPVTADQHTALSAKFGKLKSLNRGYVRLRQVKST
                     EELDWLRVGAYFSDLGMAALRDGAKPGLNEREFGDVVERAYIRHGAIHVIHYFGVTSM
                     RAPDLAVPRQF"
BASE COUNT          405 a          671 c          715 g          457 t
ORIGIN      
        1 ttgaaaccag cggcacgacg gcgatcctgg tcacgcattc gatcgaagaa gcgattctgt
       61 tggccgatac gatcctggtg atgaccgcgc ggccagggcg tattgccgcg cgcatcgagg
      121 tcgagctgcc gcggccgcgc agcgtggcaa cgacgcggga tccggggttt tcagcgctgt
      181 ttgaccggat ctacggactg ctgcgcgacg aagtcatgcg cgcgatggtc agcgggcagg
      241 gggatggcca atgagtattt cacccctggc aaaggacgac gcgccggcgc ggacgaaatc
      301 cggttatgcg ccgctcgacg atccccgcgt gctcggctgg ggctcgatcg ttctggtgct
      361 ggtcgtgtgg gagctgaccg cgcgcctgtt caatgtatcg gcgctttatc tgccgcggcc
      421 atcgcagatc gtcgtcgcgc tgatcacgtt gttcggaacc ggaggtctcg cctacgacct
      481 tggcgtcacg ctgtggcgca tcttcgccgg cttcgccatt gcgctggtga gcggaacgtt
      541 gctcgggctc tggatcgcga catcttttcg cgcgcgcgca gtagcggata tgttcatcgc
      601 ggcactctat ccgcttccga aaatcacgct tattccgctc ctgattatct ggctcgggac
      661 cggggggccg ttcatgctca caatcagctt tctcggcgcc atcttcccga tcgtgatcaa
      721 cacggtcctt ggcgtaagcc aatgcgatca gggcctcgtc ctcgcagcgc gcgatctcgg
      781 cgccacgccg ccgcagatca tgcggcgcgt gctgttgccg agcgcgatcc cgtcgatctt
      841 tgccggcgtg cgcctgggac ttggcgtttc gattattctt gtggtcgctg cggaaatggt
      901 ggtcgggaag gccgggcttg gcgcgcggct ttatctcgcc gggcaaatcc tggagacgga
      961 gcaggtcttt gccatactga ttgtcctcgc catgttgggt atcatcgtga ccaaggtaca
     1021 ggatgcagtc gatgccgtgc tgggcaattg gcgcgtcgat tgaacaggca aaagaatcgg
     1081 aaaaaaatcg gaaaggagtg ctcatatgac tgtcatgaca aagccggaca agacgttccg
     1141 tctcgatccc gttcatccaa agcccgaaga ggcgcacacg atgccatacg cgccggccgt
     1201 ccacattgtc ggtgcctgcg acctgatgtt tgtttcgggc gccacgtcgt cgccgctcta
     1261 tcacaagcac ccgcacgtcg atgaagagca cgtgcagccg cacgcgatcg aggagcagac
     1321 agagcgcgca atggcgtcga tcaagtctat tctcgatcac gtgggcgcca gctggcgcga
     1381 cgtcgtgaag attacgaaat atctcaccga tttccgcgat gctgatgcca tgcatcgcac
     1441 catgggcaag tatttcggcg attggcgacc ggccagcaca acggtctgca tcaaccagct
     1501 gtcctcaccg ggcgcacgtg tcgagcttga catgatcgtg gcgttgccaa agaaggcaac
     1561 gtgacgcttc ctccgcatct cgcacgcatc gtcgaagccg aatacccgcg cttctccgaa
     1621 caggagatga cgcggcgccg cgcggctgtc gaaaagttgc tcgagcaggc cggatgcgac
     1681 catctggtat tttgcggcgc caatcgcctt ggctcggccg tgcagtggct cacgcaatgg
     1741 ccggtgacag cggaggccgt gggtgtgttg acgccgcgcg agcgagacgc gctgttcgtg
     1801 caatatgtca atcatgcgcc gcaagccggc atacttgccg acaaggccga tgtggcctgg
     1861 ggcggcgatt cgtcaatcgc tgcggcgatc aagacgctcg aacgacgcgg agcgcgcgtt
     1921 gatcgcgtcg cgacgctcgg cccggtgacg gccgaccagc acaccgcgct gtcggcgaaa
     1981 ttcggaaagc tcaaaagcct taatcgcggt tacgtccgcc tccgtcaggt gaaatcaacc
     2041 gaggagctcg attggctgcg cgtcggcgct tatttcagcg atctcggcat ggctgcactg
     2101 cgcgatgggg caaagcctgg cttgaatgaa agggaattcg gcgacgttgt cgagcgcgcc
     2161 tacattcgtc acggggcgat tcacgtcatt cactatttcg gggtcacgtc gatgcgggcg
     2221 cctgatttgg ccgtgccgcg ccagtttc
//
LOCUS       DASSRL010000345         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_568997, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000345 DASSRL010000000
VERSION     DASSRL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568997"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFL49_07025"
     CDS             <1..263
                     /locus_tag="VFL49_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911765.1"
                     /translation="RMGFMDLGYPEFWWVIAALAIVTVLTVQGLGYLLPTNLRLYFEV
                     QKPQPDAQKIGRWMKTYVRVVALQGTMQVAIIVVMARFATGF"
     gene            263..1432
                     /locus_tag="VFL49_07030"
     CDS             263..1432
                     /locus_tag="VFL49_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET7911766.1"
                     /translation="MMALQQNTDHIQTTHIGSLPRPHDLLDLLKAKYSGQPCDENRLN
                     SVLTQSVADCVRKQVECGIEIVTDGEYSKPGFFTYVRERFDGFESRPRQKLAIFQQEI
                     AAFPDYYAQYFREAMMGGAILPLVPVVCTGPVTYKGEARLQADIANVKAAAKAVGVPD
                     RQVFLPATAPAGVGINEYYKSEEEYFHAVAAELNKEYRAIIAAGILLQVDDPFLSDIF
                     VEPDLDENQKRRRAAIYVEATNAALKGIPSERVRFHTCYGINEGPRIYEANLAQVMDY
                     VLKVDAGSFSFEAANPRHEHEYHLFEHVKLPDGKVLCPGVVTHASNIVEHPEWIAERL
                     LRFAKLVGRENVMAGADCGFSSQALYKTEVHPTVVWEKFKAMRAGADIASKRLWN"
     gene            complement(1475..3424)
                     /locus_tag="VFL49_07035"
     CDS             complement(1475..3424)
                     /locus_tag="VFL49_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HET7911767.1"
                     /translation="MSDDIHKAAAGPRSKGARCVIFAAASLVTLGSIGFALQFYRNVL
                     GLLLYNEQFLAGMLALGIALIYLTQPVRRGMSRPDVPWYDWLLAALSFAVGVYIAVRY
                     PALSEDMTARPIDGLIVAFITIPLVLEALRRSVGLALTIIVLVFLVYAPLGQYIPGPL
                     AGLPVSVAQLAFYQVWDPGSMLGLPLMVGATVVIAFVFFGNLLFASGGSAFFTDIALT
                     LMGRYRGGSAKIAVTASCLFGMISGSAVSNVASVGVLTIPLMRRGGYPAHVAAAIESV
                     ASTGGQLMPPVMGAAAFLMAEFLQIPYRDVAVAAALPALLYYYALFIQADLLAARTGL
                     TSVKQDVPPLGQVLRQGWHFLLPFGLLVYALFWLNWSPELSALAASAVLVVTGPTLGY
                     GSKKLGLGEFIDAFRSTGIASLDLLIITAAAGFIIGVLNITGLSFALTLLLVEIGSGN
                     LWLLLVLAAIVSIILGMGMPTVGVYVLLATLVAPAMVKIGMSPMASHMFVMYFGMMSM
                     ITPPVALAAYAAASLAHTDPMKTGWTAVRFGWIAFVIPFLFVRAPSLLLEGSLASIVM
                     ATITAVLGVWLICAGFAGYAVRPLSMPMRIGFGIAGLLLFIPADAVPHGEWTDIVGLI
                     FGAILLAREVVASRTKRLAATSPKI"
     gene            complement(3457..4437)
                     /locus_tag="VFL49_07040"
     CDS             complement(3457..4437)
                     /locus_tag="VFL49_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7911768.1"
                     /translation="MTKARLGILAASAVAAAITAATPASAQIYSLGTGKQGFFTYSAG
                     AAIAKVAADGRLNLRVKPFGGTSAYVPGVNAGEQQFGLANELETHYAVTGEVIYKDKP
                     QPDLRVVAVLTPLYSEFFVRKDSPIKTIADLKGKRVPSDYASQRVLDVLTKGSLANGG
                     LSYDDVHKVPVPNVVGGADEFAKGNADVFMFALGSGKVSEVDAQVGGVRVLPVDHSKE
                     AMDRLRKFIPVAYATEVKPGKVNTGIEEPTWVYAYDYLVLANSKVSDDVVYKLAKLMH
                     DHKAELAANFGALNGFDPKRMAKDMGPVQFHPGAIKFYKEIGQWPPKSGS"
BASE COUNT          920 a         1381 c         1456 g          736 t
ORIGIN      
        1 tgcgcatggg cttcatggat ctcggctatc cggaattctg gtgggtgatc gccgcgcttg
       61 cgatcgtcac cgtgctcacc gtgcagggac tcggctatct gctgccgacc aacctgcggc
      121 tttatttcga ggtgcagaag ccgcaaccgg atgcgcaaaa gatcgggcgc tggatgaaaa
      181 cctacgtgcg cgttgtggcg ctgcagggga cgatgcaggt cgccatcatc gtcgtcatgg
      241 cgcgtttcgc caccgggttc tgatgatggc gttgcagcaa aataccgatc acattcagac
      301 gacgcatatc ggcagcctgc cacggccgca tgatctcctc gatctcctga aggccaagta
      361 ttccggccag ccctgcgacg agaacaggct gaattcggtc ctgacacaat cggtggccga
      421 ctgcgtgcgc aagcaggtgg agtgcgggat cgagatcgtc accgacggcg aatactccaa
      481 acccggattt ttcacttacg tccgtgagcg cttcgacggg ttcgaatcgc ggcccaggca
      541 aaagctggcg atcttccagc aggagatcgc cgcctttccc gactattacg cgcagtactt
      601 cagggaggcg atgatgggcg gcgcaatcct gccgctcgtg ccggtcgtct gcaccgggcc
      661 ggtgacatac aaaggcgagg ccaggctgca ggccgacatc gcgaatgtga aagccgccgc
      721 gaaagccgtc ggcgtgcccg accggcaggt ttttctgccg gccacggcgc cggcgggggt
      781 tggcatcaac gagtactaca aaagcgagga ggagtatttc cacgccgtcg ccgccgagct
      841 caacaaggaa taccgcgcga ttatcgcggc aggcatcctg cttcaggtcg acgatccgtt
      901 cctgtcggac atctttgtcg agccggacct tgacgaaaac cagaagagaa ggcgcgcggc
      961 aatttatgtc gaggcaacga atgcggcact gaagggaatt ccgtcggagc gcgtcaggtt
     1021 tcatacctgc tacggcatca acgaaggacc ccggatttac gaggcaaacc tggcccaggt
     1081 tatggactat gtcctcaagg tcgatgccgg ttcgttcagc ttcgaagccg cgaacccgcg
     1141 ccacgaacac gaataccacc tgttcgagca cgtaaagctg ccggacggaa aagtcctttg
     1201 ccccggcgtc gtcacccacg cgagcaacat cgtcgaacat ccggaatgga ttgccgaacg
     1261 gctgcttcgc ttcgccaaac tcgtgggccg cgaaaacgtg atggccggcg ccgactgcgg
     1321 gttctcctcg caggcgctct ataaaaccga ggtccacccg actgtcgtgt gggaaaaatt
     1381 caaggcgatg cgcgcgggcg cggacattgc cagcaagcgg ctatggaatt gagcgcgata
     1441 cctgacgctg gagaagcgaa gcgcgttcgg cggttcaaat cttgggcgaa gtggccgcga
     1501 gccgctttgt acggctggcc accacctcgc gggcgagaag aatggcgccg aaaatcaggc
     1561 caacgatgtc tgtccattcg ccgtgtggaa cggcgtcggc cgggatgaac agcagcagac
     1621 cggcgatccc gaagccgatg cgcatcggca tcgacagcgg ccgcacggca tagccggcaa
     1681 agcccgcgca gatcagccac acaccgagga cagccgtgat cgtcgccatg acgatggagg
     1741 cgaggctgcc ttccagcagc agcgacggcg cacggacgaa gaggaatgga atgacgaagg
     1801 cgatccagcc aaaccgcacc gcggtccatc cggtcttcat cgggtcggtg tgcgccagcg
     1861 aagcggcggc gtaggcagcg agcgccaccg gcggcgtgat catcgacatc atgccgaaat
     1921 acatgacgaa catatgcgac gccatcggcg acatgccgat cttgaccatc gccggcgcga
     1981 ccagcgtcgc cagcagcacg tagacgccga ccgtcggcat gcccatgccg aggatgatcg
     2041 acacgattgc ggcgaggacc agcagcagcc acaggttgcc cgagccgatc tcgaccagca
     2101 gcaacgtcaa tgcaaagctc aggccggtaa tgttgagcac gccgatgatg aagcctgcgg
     2161 ccgccgtgat gatcagcagg tcaagcgaag cgatgccggt gctgcggaag gcgtcgataa
     2221 attcaccgag accgagcttc ttgctgccat agccgagcgt cggcccggtg acgaccagca
     2281 cggcggaagc ggcgagagcc gacagttccg gcgaccagtt gagccagaac agcgcgtaga
     2341 ccagaaggcc gaaaggcagc aggaagtgcc agccctgcct gagcacctgc ccgagcggcg
     2401 gcacgtcctg cttgacactg gtcaggccgg tgcgggcggc cagaaggtcc gcctgaatga
     2461 acagcgcata ataatagaga agggcgggaa gcgccgccgc gaccgcgacg tcgcgataag
     2521 gaatttgcag gaattccgcc atcaggaagg cggccgcgcc catcaccggc ggcatcagct
     2581 gcccgcccgt cgaagcgaca gattcgatgg cggcagcgac atgggcgggg tacccgccgc
     2641 gccgcatcag cggaatggtg aggacgccga ccgacgcgac gttgctcacg gcgctgcccg
     2701 agatcatgcc aaagaggcag gaggcggtga cggcgatctt ggcggagccg ccgcggtagc
     2761 ggcccatcag cgtcagcgcg atgtcggtga agaatgccga gccgccggag gcaaacagca
     2821 ggttgccgaa aaagacgaag gcaatgacga cggtcgctcc caccatcagc ggcagcccga
     2881 gcatgctgcc gggatcccac acctggtaga atgcgagttg cgcaacgctg accggcaggc
     2941 cggcaagcgg tcccggtatg tattgcccga gcggagcgta gacgagaaaa acaagcacga
     3001 tgatcgtcag cgccagaccg accgagcgcc gcaaggcctc aagcacgagc ggaatcgtga
     3061 tgaatgcaac aatcaggccg tcgatcggcc gcgccgtcat atcttcggag agcgccgggt
     3121 atcgcaccgc gatgtagacg ccgacggcga agcttagagc cgccagcaac cagtcgtacc
     3181 aaggcacgtc cgggcgcgac attccgcgcc gcaccggctg ggtcaggtag atgagcgcaa
     3241 tgccgagcgc gagcatgccg gcgaggaact gctcgttgta cagcagcagc ccgagcacat
     3301 tgcggtaaaa ttgcagcgcg aagccgatcg agccgagcgt gaccaggctc gcagcagcga
     3361 aaatgacgca gcgggcgcct ttgcttctcg ggcccgccgc agctttgtgg atgtcgtcag
     3421 acacggcgcg gtttcgtgtg ccttgtcgtc aggcgatcag ctcccgctct tcggcggcca
     3481 ctggccgatt tccttgtaga acttgatggc gccggggtga aactgcaccg gccccatgtc
     3541 cttggccatg cgcttgggat cgaaaccgtt gagcgcgccg aaattggcgg cgagctcggc
     3601 cttgtggtca tgcatcagct tggcgagctt gtagacgaca tcgtcgctca ccttgctgtt
     3661 ggcgagcacc aggtagtcgt aggcatagac ccaggtcggc tcctcgatac cggtgttgac
     3721 cttgccgggc ttgacctccg tcgcataggc gaccgggatg aacttgcgca gccggtccat
     3781 cgcctccttc gagtgatcga ccggcaacac ccgcacgccg ccgacctgcg cgtcgacttc
     3841 ggagaccttg ccggatccga gcgcgaacat gaaaacatcg gcgttgcctt tggcgaattc
     3901 gtcggcgccg ccgacgacgt tcggcacggg caccttgtgc acgtcgtcgt agctgaggcc
     3961 gccgttcgcc agcgagccct tggtgagcac gtcgagcacg cgctggctcg cgtagtcgct
     4021 cgggacgcgc ttgcccttga gatcggcgat ggtcttgata ggcgaatcct tgcggacgaa
     4081 aaactccgag tagagcggcg tcagcacggc gaccacgcgc aggtcgggct gcggcttgtc
     4141 cttgtagatg acctcgccgg tgaccgcgta atgggtctcg agctcgttgg cgaggccgaa
     4201 ctgctgctcg ccggcgttga cgccaggcac ataggcgctg gtgccgccga acggcttgac
     4261 gcgcaagttc aatctgccgt cggccgctac cttcgcgatg gccgcaccgg ctgagtaggt
     4321 gaaaaagccc tgcttgcccg tgccgaggct gtaaatctgc gcgctcgccg gcgttgcagc
     4381 cgtgatcgcg gcggctacgg cgcttgcggc caatatgccc aatcgtgcct tggtcatcga
     4441 atcctccctg gaatttttct gtgttgaggg agagtacaca ccggcgcggc atc
//
LOCUS       DASSRL010000346         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_271289, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000346 DASSRL010000000
VERSION     DASSRL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271289"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VFL49_07045"
     CDS             complement(<1..1013)
                     /locus_tag="VFL49_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7911769.1"
                     /translation="MRTLLCTVLFSLCLAVGAKAAPNIVVILTDDQEDTGSMAYLPNV
                     QALAAQGVTFKNSFVDFSLCAPSRSSFLTGQAAHNDGIKSNKLDKGGGWDTFKQNEAN
                     DLAVWLKAAGYRTALIGKYENGYGAGKPPRLASAAYWSNALNGWFGIGAPDKQHWVPP
                     GWDLWYAFTKVRYYDYTINENGKLLNFGHEPADYSTDVLKGRAVRFINDQSGSAAPFF
                     MLIATKAPHGQGEEGQKEPAVPAPAYQNAFADIKLPKTPAYDEEDVSDKGSLVARAPR
                     LNDAFKDQIEKSYRAELQSLRSVDDLVGDVVSALDKAGKLDNTVIIYTSDNGYVYGDH
                     RLFG"
     gene            complement(1221..>2281)
                     /locus_tag="VFL49_07050"
     CDS             complement(1221..>2281)
                     /locus_tag="VFL49_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7911770.1"
                     /translation="GAWLAWFGSWINVEFKGTTVGNPRDWVPPGWDLWYAFTGTRVRY
                     YDYWINENGTILSFDHRPNDYSTDVLAERAVRFINDEAESQAPFFMFIAPKTAHAQGA
                     RAIPAPKYEQAFNDVQLPENPAFNERDVHRKALKAPRVGLKTKEDLELSYRAELQSLQ
                     SVDDLVAAVVDALRDTNKLDNTVLIYTSDNGFLFGDHRLIGKSAAYEGSIKVPLLMRG
                     PGIPERETRDQLVNNLDLVATILDLAGAKPGVPLDGKSLKPLFADAKRPWRSSILIES
                     PVTRFEKASNRYTGVRTATRKYVKYDSGFEELFDLSADPHELKNKAKDPAYASDLATL
                     RGVEATLKTCVGESCWVP"
BASE COUNT          391 a          691 c          734 g          465 t
ORIGIN      
        1 ccgaacaagc ggtgatcgcc atacacatag ccgttgtcgg aggtgtagat gatcacggtg
       61 ttgtcgagct tgccggcctt gtcgagtgcg ctcactacgt cgcctacgag atcgtcgacc
      121 gaccgaagag actgcaactc ggcgcgatag ctcttctcga tctgatcctt aaacgcatcg
      181 ttgagccgcg gcgctctcgc caccagggag cccttgtcgc tgacatcctc ctcatcgtag
      241 gcgggcgttt tcgggagctt gatgtcggca aacgcgttct ggtaggcggg cgccggcacc
      301 gcaggttcct tttgcccttc ctcaccttgc ccgtgcggcg ccttggtcgc gatcagcatg
      361 aagaaaggcg cggccgagcc cgattggtcg ttgatgaagc gcacggcccg gcccttcagc
      421 acgtcggtcg agtagtcggc gggctcgtga ccgaagttga gaagcttgcc attctcgttg
      481 atggtgtagt cgtagtagcg gaccttggtg aaggcgtacc aaaggtccca gcccggtggc
      541 acccagtgtt gtttgtccgg ggcgccgatc ccgaaccagc cattgagggc gttcgaccag
      601 taggcagcgc tcgcgaggcg gggcggtttg ccggcgccgt agccgttttc atacttgccg
      661 atcagcgccg ttcggtagcc tgcagccttc agccacaccg cgaggtcgtt cgcttcattt
      721 tgcttgaaag tgtcccagcc gccgcctttg tccagcttgt tcgacttgat gccgtcattg
      781 tgggccgcct ggccggttag gaacgacgac cggcttggcg cgcaaagcga aaagtcgaca
      841 aagctgttct tgaaggtgac gccctgcgcg gccagcgcct ggacgttcgg cagataggcc
      901 atggagcccg tgtcttcctg atcgtcggtg aggatgacga cgatgttggg cgctgctttg
      961 gcgccgacgg cgagacagag agagaagagg actgtgcaaa ggagtgtgcg catggaagtt
     1021 gccccgaatt gccccgccgt cccgaatgcg ccttgcgatc agaccggctt gccggcggat
     1081 ggcgccaccg ggcgcggtct ttttcagggc aggaatcggc actgcgtcac tcggtccgac
     1141 cgcccgcgga cctgcgcctg ccctgaaagc ggccaggttc ctggagttcg agaggatgcg
     1201 gcagattgcc gtcgcgccgc ctaaggcacc cagcagctct caccaacgca ggtctttagc
     1261 gtcgcctcga cgccgcgaag cgtcgcaagg tcgctcgcat aggcgggatc tttcgccttg
     1321 ttcttgagct cgtgagggtc ggcagagaga tcgaacagct cctcgaagcc actgtcgtat
     1381 ttcacgtatt tcctcgtggc ggtgcgcacg ccggtatagc ggttcgacgc cttctcgaag
     1441 cgggtcaccg gactttcgat caggatcgag ctccgccaag gcctcttggc gtcggcaaag
     1501 aggggcttga gcgacttgcc gtcgagcggg acgcccggct tggcgccagc cagatccaaa
     1561 atggtcgcca ccaaatcgag attgttgacg agttgatcgc gcgtctcgcg ctccggaatg
     1621 cccgggccgc gcatcagaag aggcaccttg atcgaaccct cgtaggccgc ggacttgccg
     1681 atcagacggt ggtcaccgaa taggaagccg ttgtcggagg tatagatcag caccgtgttg
     1741 tcgagcttgt tggtgtccct tagcgcatcg accacggcgg cgacgagatc gtccaccgat
     1801 tgcagcgact gcagctcggc gcggtagctc agctcgagat cctccttcgt cttgaggccg
     1861 acgcgcggag ccttcagtgc tttgcggtgc acgtcccgct cgttgaaggc cgggttttcc
     1921 ggcagctgaa cgtcgttgaa ggcctgctcg tatttcggcg ccggaatggc gcgggcccct
     1981 tgcgcgtgcg cggtcttcgg cgcgatgaac atgaagaacg gagcctggct ttccgcctcg
     2041 tcgttgatga agcggacggc gcgctcggcg agcacgtcgg tcgaataatc gtttggacgg
     2101 tgatcgaagc tcaggatcgt gccgttctcg ttgatccaat agtcatagta tctcacccgc
     2161 gtcccggtga aggcgtacca aaggtcccac ccaggcggca cccagtcgcg cggattgccg
     2221 acagtggtgc ccttgaactc cacattgatc cagctgccga accaggcgag ccacgcgcca
     2281 t
//
LOCUS       DASSRL010000347         6232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_796304, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000347 DASSRL010000000
VERSION     DASSRL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796304"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..995)
                     /locus_tag="VFL49_07055"
     CDS             complement(57..995)
                     /locus_tag="VFL49_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7911771.1"
                     /translation="MRREGSAFQGLGVVVLKELSDHFASARMRVLEWLVVLTALAAVY
                     GAIQQVRDVTAEDPFLFLRLFTTAREPLPSFVSFISFLVPLMAIGLGFDAVNGEHSRR
                     TLSRILSQPIYRDALLFGKFLAGMLTLSVSLISLWLLVIGLGLIMLGVPPGGEEMARA
                     FLFLVVTIVYAGVWLALAMLFSVIFRSAATAALVTLGIWLFVSVIWPALSSAIAEALF
                     STADQATQIVGGQMLARLSPSTLFGECVLALLHPTTRTLGPIYLSQLQGAVIGAPLPL
                     WDSVMVAWPQVVGLIAATILLFVAGYVAFQRQEVRA"
     gene            complement(985..1935)
                     /locus_tag="VFL49_07060"
     CDS             complement(985..1935)
                     /locus_tag="VFL49_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911772.1"
                     /translation="MNEPVIEAKGLTKRYRTATAVDSVTFDVAAGEIFGMLGPNGAGK
                     TTTILMLLGLSDVSAGYARVFGHDPKREPLAIKRRVGYLPDSVGFYDHLTAAENLHYT
                     ARLMNLEAKARAGKIKVALERVGLSDVANKKVATFSRGMRQRLGLAEIVMKDARAAIL
                     DEPTNGLDPQASVELLELIRSLKAQGVTILLSSHLLDRVQSVCDRVALFNEGRIALMG
                     TVADLGRQVLGGGFAVEVEADGGTDIVQRLSSLSGVRSVGQPAPGKWRLTCDRDLRAE
                     TAATVIQAGARLSKLSLDEPSLETVYRSYFQGVQGVSHAA"
     gene            complement(1932..3092)
                     /locus_tag="VFL49_07065"
     CDS             complement(1932..3092)
                     /locus_tag="VFL49_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HET7911773.1"
                     /translation="MRYRLPALVAIFFALTAAASADPPKDIKGLFLLTDYPAVTVQPG
                     TTATINLRLRNYDLAPERLSLSVSGVPTGWTATLLGGGQPIAAAMPAADASVSLDLRL
                     DIPKDAGTGTHTLTVTAEGDAQHVSLPVQVTLAKQLPAKLTLTPQLPELRGSSHSNFE
                     YTLAIKNDSGKKLLVSLAAEAPHNFDTSFTEAYGTQQLTAIPVDAGKSKDVKLKVTAP
                     DTIDAGHYPVKVHVAAEDARANAEVALDITGQPKLALAGREGLLSARATAGRDSTIPV
                     TVTNTGTAPADNIQLSGTAPSGWKVTFEPKTIDRIAPNQHKEVQAVIRPSDKAIAGDY
                     VTTLTASSRGETGTANFRVTVTTSTMWGVAGVGIIGAALLIMVGAVARYGRR"
     gene            complement(3258..3941)
                     /locus_tag="VFL49_07070"
     CDS             complement(3258..3941)
                     /locus_tag="VFL49_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7911774.1"
                     /translation="MALALIIGNKNYSSWSFRPWIAMKVAGIAFDEKVIPLYEPGSRE
                     QILQYSPAGKVPVLVDGDQHIWESLAILEYLAERFPKQNLWPEDPRARAQARVVATEM
                     HAGFQPLRKSCPMNMWLPVKPRPQPEEVMANVRRIEFLWADCRARFGQKGPFLFGGFG
                     VADAMYAPVVARLHSYGLKVGEGARNYMDAVMALPAWAEWRTAALKEKWILKDDEPEW
                     PLVRGVPVA"
     gene            complement(4061..4456)
                     /locus_tag="VFL49_07075"
     CDS             complement(4061..4456)
                     /locus_tag="VFL49_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7911775.1"
                     /translation="MPQNITIGYKALCDAAEREIETLPVDEAIKLTGRNDTVLVDIRD
                     IRELQREGKVPGAFHCPRGMLEFWIDPQSPYHKAIFADDKKFVFFCAGGLRSALAAQT
                     AQRMGLKPVAHIGGGFGAWKKAGGPVEAA"
     gene            4570..5265
                     /locus_tag="VFL49_07080"
     CDS             4570..5265
                     /locus_tag="VFL49_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HET7911776.1"
                     /translation="MTVLTLPDLAAIIWFLGAWIAYSMVIEKTAKGRRTLNALMNGYR
                     DEWMAQLLVRDMRMVDAQVTAALQNGTAFFASTSLIAIGGALTLLRSSEEILTAISQL
                     PFGATPSRDLWDLKMMGLIIIFVYAFFKFAWSYRLFNYFAIMVGAAPPSSEKNTAAAQ
                     EFAHRAAHLCADAGRHFNRGQRAFFFALGYLGWFLGPAPFALSTAGILVVMWRRQFAS
                     DARKAFDRAEEHK"
     gene            complement(5288..5518)
                     /locus_tag="VFL49_07085"
     CDS             complement(5288..5518)
                     /locus_tag="VFL49_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911777.1"
                     /translation="MRAIRAPEFAFACSRTIEAMPFKKGAPMALNFRSILPSGLTSQF
                     GAAHADDWTITAAAASLGLLVVAAIAVLMGMA"
     gene            complement(5641..5934)
                     /locus_tag="VFL49_07090"
     CDS             complement(5641..5934)
                     /locus_tag="VFL49_07090"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HET7911778.1"
                     /translation="MPCRYWAKGPLAANDHAPHYARLYFAIGMRRQSIGSCQMADSKR
                     NRGKSDRSRVSGSQRSEVYYVARKYSVSADTVRGVIKRVGNSRKKVYAALDKM"
     gene            complement(5975..>6232)
                     /locus_tag="VFL49_07095"
     CDS             complement(5975..>6232)
                     /locus_tag="VFL49_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET7911779.1"
                     /translation="EPLLALPAHTLVYADPVGENHVRARLRSGDGKFVNAIAFRAMGQ
                     PLGRALLDSRGRVLHAAGHIAVDRWQGEERVQLRLADVAPA"
BASE COUNT         1315 a         1965 c         1733 g         1219 t
ORIGIN      
        1 tgattttgca tctaaagacg aaatagcctt gaccggaccc gaagtcgctt aaccgcttac
       61 gccctcacct cctgacgttg gaacgcaacg tagcccgcaa caaacagaag gatcgtcgct
      121 gcaatcaacc cgacaacttg cggccaggca accatcacac tgtcccaaag cggcagcggc
      181 gcgccaatga ccgccccctg tagttgactc agataaatag ggccaagcgt gcgcgtcgtc
      241 gggtgcagta acgccagcac gcactcgccg aacagagtgg atggcgacag ccgggccagc
      301 atctgaccgc cgacgatttg cgttgcctgg tcggcagtcg aaaacagcgc ctccgcaatc
      361 gccgacgata gagccggcca tatgacgctc acgaatagcc aaatcccaag cgtcacaagc
      421 gcggccgtcg ccgccgaacg gaatatgacc gagaacaaca tcgcgagagc caaccacacg
      481 ccggcataga cgatggtcac aaccagaaag aggaaggcgc gcgccatttc ctcgccgccc
      541 ggaggcaccc caagcatgat caggccaaga ccgatcacaa gcagccaaag cgagatcagg
      601 ctcaccgaca gtgtcagcat ccctgcgaga aatttcccga acagtagcgc atcccgatag
      661 atcggctgtg acaaaatgcg cgacagtgtc cgccggctat gctcgccatt caccgcatca
      721 aatcccaatc caatcgccat cagcggcacc agaaaactga taaacgacac gaatgacggt
      781 aacggctcac gtgccgtcgt gaacaggcgc agaaatagaa atggatcctc cgcagtaaca
      841 tcgcgaactt gctggatggc gccatagacg gcagcaagcg ccgtcaacac gacaagccat
      901 tccagcacac gcatgcgtgc gctggcaaaa tggtctgaca gttctttcag cacaacaacg
      961 ccgaggccct gaaaggcaga tccttcacgc cgcatgagag acaccctgca cgccctgaaa
     1021 atagctcctg tagacggttt cgaggctcgg ctcatcaagc gaaagtttgc tgagccgcgc
     1081 tcctgcctgg atcactgtcg ccgcagtttc cgcgcgcaag tcacgatcgc atgtaagccg
     1141 ccattttccc ggcgccggct gccccacgct gcgtactccc gacaatgacg agagacgctg
     1201 aacgatgtct gttccgccgt cggcctcgac ttcgaccgcg aagcctccgc cgaggacctg
     1261 acgccccaga tcggcgaccg tgcccatcag cgcgatacga ccttcattaa acagggcgac
     1321 gcggtcacac acgctttgca cccgatcaag caggtgggat gacaagagaa ttgtcacccc
     1381 ttgcgccttc aaagaccgaa taagttcaag cagttcgacg cttgcctgcg gatcaaggcc
     1441 attggtgggt tcgtccagaa ttgctgcccg cgcatccttc atcacaattt ctgcaaggcc
     1501 gagccgctgg cgcatgccgc gcgaaaacgt cgccaccttt ttgtttgcga cgtcgctcaa
     1561 gcctacgcgc tcgagcgcaa ccttaatctt gccggcgcgc gcttttgctt ccagattcat
     1621 cagtcgcgct gtgtagtgca gattctctgc agcggtcaga tgatcataga agccaacgga
     1681 gtcgggaaga tagccgacgc gccgtttgat cgccaacggc tcccgcttcg gatcatgacc
     1741 gaacacgcgc gcataccctg cgctgacgtc gctcaggcca agcagcatca gaatcgttgt
     1801 tgtcttgccc gcgccgttcg gtccgagcat tccgaaaatc tcgccggcgg cgacgtcaaa
     1861 tgtgacgctg tcgacagcag tcgctgttcg atagcgcttc gtgaggcctt tcgcctcaat
     1921 caccggctcg ttcatcgccg accgtagcgc gcaaccgcgc caaccatgat gaggagggcc
     1981 gccccgatga tgccgacacc ggcgacgccc cacattgtgg acgttgtgac cgtgacgcgg
     2041 aaattggcgg tgcccgtctc gccgcgactg gaagcagtca aggtcgtgac atagtcgcct
     2101 gcgatcgcct tgtcggacgg acggatcact gcctgcactt ccttgtgctg attgggcgcg
     2161 atgcgatcga tcgtcttcgg ctcgaaggtc accttccacc cgctaggcgc agtccctgac
     2221 agctgaatat tgtcggcggg agcagtcccg gtattggtga ccgtcacggg tattgtgctg
     2281 tcgcggccgg cggtcgcgcg ggcgctcaaa agcccttccc tgccggccag cgccagtttt
     2341 ggctgtcctg tgatatcgag cgccacctcg gcgttggcgc gggcatcttc agccgcaaca
     2401 tgcaccttca ccggatagtg gccggcatcg atcgtgtcgg gcgccgtgac cttgagcttc
     2461 acatccttgc tctttcccgc atccaccgga atggcagtga gttgttgcgt tccataggct
     2521 tcggtgaaag acgtatcgaa attgtgcggc gcctcagctg caaggctgac taacaatttt
     2581 ttgccgctgt cattcttgat cgccagtgtg tactcgaaat tcgagtgaga tgagcctcgc
     2641 agttcgggta attgcggagt cagcgtcagc ttcgcaggca attgcttggc gagcgtcacc
     2701 tggaccggta gcgacacatg ctgtgcgtcg ccctcggcgg tcacggtcag cgtatgtgtg
     2761 ccggtgccag cgtctttcgg aatatccagc cgcagatcga gcgaaacgct tgcatcagcc
     2821 gcgggcatgg ctgcagcgat cggttgcccc ccgcccagca gcgtggctgt ccagcctgtc
     2881 ggcacaccgg aaacggagag cgataaccgc tcaggagcaa gatcgtagtt tcgaagcctc
     2941 aagttgatcg tagcagtcgt gccgggctgc acggttaccg ccggatagtc ggtcagaagg
     3001 aagagccctt taatatcctt cgggggatca gcactggcag cggcggtgag cgcaaaaaat
     3061 atagcgacga gcgcaggcag acgatagcgc atgacgagca actccgtacg gggtcaataa
     3121 accccgcaag aacgtcgccc ccaaagcgga agacacgact cgttgtcgtg aaaggaatct
     3181 tccacatcgt gcgcatgatt ttgacaaaat tgggactgcc cgcgccgcaa accgcggcac
     3241 aactgcgcac ccacccatta cgcgaccggg accccgcgta ccaaaggcca ctccggctca
     3301 tcgtccttta aaatccactt ctccttgagt gccgccgtcc gccattcagc ccaggccggc
     3361 agggccatca ccgcgtccat ataattgcgc gcgccctcgc caaccttcag gccatatgag
     3421 tgcaatcgcg cgacgacggg cgcgtacatg gcgtccgcca cgccaaagcc cccgaaaaga
     3481 aacggtccct tttgcccgaa gcgcgccctg caatccgccc acaagaactc tatccgccgc
     3541 acattagcca tcacctcttc cggctgcggc cgcggcttca ctggtaacca catattcatt
     3601 gggcaactct tgcgcagcgg ctggaacccg gcatgcatct cggtcgccac aacgcgagcc
     3661 tgagcccgcg cacgaggatc ttcaggccaa aggttctgct tggggaatct ctcggccaaa
     3721 tattcgagaa tggcgaggga ttcccagatg tgctgatcgc cgtccacgag cacgggcacc
     3781 ttgccggccg gtgaatattg caggatctgc tcgcggctgc ccggttcata gagcgggatc
     3841 accttttcat cgaaggctat ccccgccact ttcatggcga tccagggccg gaacgaccat
     3901 gaagaatagt tcttgttgcc gataatcaat gccagcgcca tgacctctct ccgtccgcgc
     3961 tgaaattgcg ggaaggtgga aggcacaaca agtgaaattt tctgatcggc ctgagcaggc
     4021 cggaaaatac ttgctgtttc tttgcctgta cgaacttcga ctaagctgcc tccacgggac
     4081 cgccggcctt tttccaggca ccaaatccgc ctccaatgtg ggcgactggc tttagcccca
     4141 tgcgctgcgc cgtctgcgca gccagtgcag atcgcaatcc gccagcgcag aagaacacaa
     4201 acttcttgtc gtcggcgaag atggctttgt ggtacgggct ttgcggatcg atccagaatt
     4261 ccagcatccc acgcgggcaa tggaaggcgc ccggtacctt gccctctcgc tgcagttcac
     4321 gaatatcacg gatatcgacc agcaccgtgt cgttgcgccc ggtgagtttg attgcttcgt
     4381 cgactggcaa ggtctcgatc tcgcgctcgg cggcatcgca caaggccttg tacccgatcg
     4441 tgatattttg aggcattgaa ggatctccta cggcagccca tgctgacaaa gcatctgtcc
     4501 gcacgcaagg catgcggttg aagcccgcag cgcaagtggg caccatgaca tcaaacgcga
     4561 ctcgatccga tgacagttct cactctgccc gatctcgccg ccattatctg gtttctcggc
     4621 gcatggatcg cgtattccat ggtcattgag aagaccgcga aaggccgccg cactctcaat
     4681 gcgctgatga atggctaccg cgacgagtgg atggcgcaat tgctggtacg tgacatgcgg
     4741 atggtcgacg cccaagtcac cgcagcgctg cagaatggca cggccttctt tgcatcgacg
     4801 agcctgatcg cgatcggtgg cgcgctgacg ctgctgcgat ccagtgagga aatcctgacg
     4861 gcaatatccc agctgccatt cggcgccacg ccgtcgcgcg acctgtggga cctgaagatg
     4921 atgggcttga tcatcatttt cgtctatgca tttttcaagt tcgcatggtc ttatcggctg
     4981 ttcaattact ttgccatcat ggtcggcgcg gcgccgccgt cgtccgagaa gaatacggca
     5041 gcggcccagg aattcgcgca ccgcgcagca catttgtgcg ccgatgccgg tcgccacttc
     5101 aatcgcgggc aacgcgcgtt cttttttgca ctcggttatt tgggctggtt tctgggtcct
     5161 gcgccgttcg cgctttccac ggcaggaata cttgtggtca tgtggcgccg tcagtttgca
     5221 tccgacgcgc gcaaagcgtt cgaccgcgcc gaagaacaca agtgagccac accgaaacgc
     5281 cgtctggtca ggccattccc atcaaaacgg caatagcggc gacgaccagc aggcccagag
     5341 atgcggcggc tgcggtgatc gtccaatcgt cggcgtgagc cgctccaaat tgactcgtca
     5401 gccctgacgg caagattgat ctgaaattga gtgccatggg cgcccccttc ttgaaaggca
     5461 tcgcttcgat ggtgcgcgag cacgcgaacg cgaactcggg cgcacgaatc gccctcaccg
     5521 ccgagattag cccggccgca atttgccctc aatgaatggc ggccagccgc tcacaattct
     5581 gaaaacgcaa agccaacgcc ctcggtgctg caaaggcggc tgtcatcgcc acgactacag
     5641 ctacatcttg tccaacgcgg cgtagacttt ctttcgactg ttaccgacgc gcttgattac
     5701 gcctctcacg gtgtccgcgc ttacgctgta ctttctcgcg acgtagtaga cctctgatcg
     5761 ctgactcccc gacacccggg accgatctga ctttccgcgg tttcgcttgc tatcggccat
     5821 ctgacagctc cctatgctct gtcgcctcat tccgattgca aagtagagac gggcatagtg
     5881 cggcgcatgg tcgttcgctg ccagtgggcc tttggcccaa tatctgcagg gcatacttta
     5941 atcgggcgga aaacgactat cgtggttttc gctgtcaagc gggtgcaacg tcggcaagcc
     6001 gcaactggac gcgctcttcg ccttgccaac gatcgaccgc gatatggcct gccgcatgca
     6061 acacgcggcc gcgactgtcg agcaaagccc gcccaagggg ctgacccatg gcccggaatg
     6121 caattgcatt gacgaatttt ccgtcgccgg atcgcagccg tgcccgaacg tggttttcgc
     6181 cgacgggatc cgcataaaca agcgtgtggg caggaagagc aagcaatggc tc
//
LOCUS       DASSRL010000348         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1391215, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000348 DASSRL010000000
VERSION     DASSRL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391215"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VFL49_07100"
     CDS             <1..1705
                     /locus_tag="VFL49_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7911780.1"
                     /translation="DFAEACLAAGIVFVGPPASAMRSMGLKHHAKALMEKAGVPVVPG
                     YHGEMQEPKFLKQKAYEIGYPVILKPLAGGGGKGMRRVERHADFDAALEGAQREAKAS
                     FGDARVLVEKYVTRPRHIEVQIFADHHGNALHLGERDCSLQRRHQKVVEESPAPGMTA
                     ELRARMGASAIAAAKATDYVGAGTVEFIADATNGLKAGSFWFMEMNTRLQVEHPVTEA
                     VTGLDLVEWQFRIAAGEKLPLAQEQIAPNGHAVEARLYAEDPERGFLPSPGRLLDLQF
                     PIADGLRIDTGVERGSEVTPFYDPMVAKLIAHGQTRDQAFNRLATALDRTILAGPRSN
                     LGFLAALCRAAPVRKGDFDTGFIDGNLADLGAIPHRIDRAAAAFGAQWLLTQQHLPAA
                     RAPGDAPGASSSPWDADDAFQLSGTRRLALAILADGQAIVAEVVYAAEGLQLSVEGVA
                     PASDAVAVAGPNAVFVLRQGRETKVSMRDSGLADAGNHDKSGLVRAPMHGKVLALLVE
                     KGVRVTRGQRVAIVEAMKMEHTIVAPMDGVVTEIMVAKDAQVAEGAQLMAVSPLGENV
                     S"
     gene            1778..2215
                     /locus_tag="VFL49_07105"
     CDS             1778..2215
                     /locus_tag="VFL49_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HET7911781.1"
                     /translation="MSLHLIKLCVGCDSVADLEEWIKEKLKEKKRRRQKPEHIHTTRM
                     VPKRGEELTHGGSLYWVIRGQIACRERILDIRPFTDKDGVGRCRIVLDGKVVLVEPRP
                     RSAFQGWRYLEPKDAPRDLSRAAPGAARMPETMRRELRELGLL"
     gene            complement(2212..>2512)
                     /locus_tag="VFL49_07110"
     CDS             complement(2212..>2512)
                     /locus_tag="VFL49_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7911782.1"
                     /translation="DGLALSSRNLYLSSAERAVAPVLYKVLRSCASRIKRGDKIDRVL
                     NVGRIEIDLNGFNLDYLEARHAQTLAPVASVKEGPVRLLVAAKLGKTRLIDNIAV"
BASE COUNT          500 a          713 c          817 g          482 t
ORIGIN      
        1 tgacttcgcc gaggcctgtc ttgcggcggg catcgtcttt gtcggcccac ctgcttcagc
       61 catgcgcagc atgggcctca aacaccacgc caaggcgttg atggaaaaag cgggcgtgcc
      121 cgtcgtgcct ggctatcacg gcgagatgca ggagccgaag ttcctcaaac agaaagccta
      181 cgaaatcgga tatccggtta tcctcaaacc tctcgctggc gggggcggca agggtatgcg
      241 ccgcgtggag cgccacgccg atttcgatgc cgcgctcgag ggagcgcagc gggaagccaa
      301 ggcttccttc ggcgacgcgc gcgtccttgt agagaaatac gtgacaaggc cgcgacacat
      361 cgaggtgcag atctttgcgg atcaccatgg aaacgctctc cacctcggcg agcgcgattg
      421 ttcgctgcag cgccgtcacc agaaggttgt ggaggaatct ccggcacccg ggatgacagc
      481 cgagttgcgt gcgcgaatgg gtgcaagcgc aatcgccgca gcgaaggcaa cggactatgt
      541 cggtgccgga accgtggaat tcatcgccga cgccaccaac ggcctcaaag caggcagttt
      601 ttggttcatg gaaatgaaca cgcggctgca agtcgaacat cctgtcacag aagcggtaac
      661 ggggcttgat ttggtcgaat ggcagttccg gatcgcggcc ggtgaaaaac tgccgttggc
      721 gcaggagcag atcgctccaa acgggcatgc agtcgaggcg cggctctatg cagaggatcc
      781 cgagcgcggc tttttgccct cgcctggccg gctgctggac ttgcaatttc cgatagctga
      841 cggattacgc atcgataccg gggtagagcg cggcagcgaa gtgacgccgt tttacgatcc
      901 aatggtcgcg aaactgatcg ctcacgggca aacgagggac caggccttca atcggctggc
      961 cactgcgctt gatcggacga ttctggcggg tccgcggagc aatctcgggt ttcttgccgc
     1021 gctctgccgt gctgccccag tacgcaaggg cgattttgac accggcttta tagatggcaa
     1081 tcttgccgac cttggtgcga ttccgcacag gattgatcgc gccgcagctg cattcggtgc
     1141 acagtggctt ctcacccagc agcaccttcc cgctgcgcgc gcgccagggg atgcgcccgg
     1201 tgcgtcgtca tccccctggg acgctgacga cgcatttcaa ctatccggca cgcggcggtt
     1261 ggcgttggcc atactggccg atggccaggc aatcgtcgcc gaagtggtgt acgcggccga
     1321 gggtcttcaa ttgtcggtgg agggcgttgc gcccgcctcg gatgcggttg cggtcgccgg
     1381 tccaaacgcc gttttcgtac tgcgccaggg tcgcgagacc aaagtcagca tgcgcgattc
     1441 tggccttgcg gacgccggga accacgacaa aagcggcctt gtgcgtgcgc ccatgcatgg
     1501 caaagtgtta gcactcctcg tcgagaaggg cgtccgggtc acgcgggggc agcgcgttgc
     1561 catcgtcgag gcgatgaaga tggagcacac aattgtggct ccgatggatg gcgtcgtaac
     1621 agaaattatg gtcgccaagg atgcgcaggt cgcggaaggg gcccaactga tggcagtctc
     1681 gccgctgggg gagaacgtta gctaacagct ttcgtatgga ccatttgccc gttatggatg
     1741 ggggctgaca ggttacgggt tcgttgggtc tcgcaggatg tcacttcatc tcatcaagct
     1801 atgcgtcggc tgcgactcgg ttgccgacct cgaagaatgg atcaaagaga agctgaagga
     1861 aaagaaacgg cgtcgccaga agccggagca tattcacacg acgcgtatgg tgccaaagcg
     1921 cggcgaggag cttacccacg gaggctcgtt gtattgggta attagggggc agattgcttg
     1981 ccgggagcgc atcctcgaca ttcgcccttt tacggataaa gacggcgtcg ggcgttgccg
     2041 cattgttctc gacggcaaag tggtgcttgt cgaaccacga ccgcgctcgg cattccaggg
     2101 ctggcgctac ctggagccca aggatgcacc gcgggatctt tcacgcgcgg cacccggcgc
     2161 cgccagaatg ccagagacga tgcgacgcga gctgcgcgaa ttggggcttc tttagaccgc
     2221 aatgttgtca atcagccgtg ttttgccaag cttcgcggcg acgagcaggc gaacggggcc
     2281 ttccttgacc gaagcgacgg gcgccagggt ctgcgcgtgg cgtgcctcga gataatcgag
     2341 gttgaatccg ttcaagtcta tttcaatgcg acccacgttc aggacgcggt cgattttatc
     2401 gcccctttta atgcgagagg cacaacttct gaggactttg tagagaaccg gggcgacggc
     2461 gcgctccgct gaggaaagat acaagttgcg cgaggaaagg gccaaaccgt cc
//
LOCUS       DASSRL010000349         6733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1601143, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000349 DASSRL010000000
VERSION     DASSRL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601143"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..651)
                     /locus_tag="VFL49_07115"
     CDS             complement(70..651)
                     /locus_tag="VFL49_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HET7911783.1"
                     /translation="MLRRLYDWCIAAAYKPYAMWLMGAVSFAESSFFPVPPDVMLIPM
                     ALARPERAYVMAIWCTVTSVLGGVLGYAIGALLYDSIGLWLIQLYGYGDKVEAFRGAY
                     AEWGAWIILLKGLTPIPYKIVTITSGFAGYNLGLFILFSVITRGARFFLLAFLLHRYG
                     ESARHIIERRLGLWVLLGALVLVIGVVAALYLF"
     gene            complement(712..1092)
                     /gene="hisE"
                     /locus_tag="VFL49_07120"
     CDS             complement(712..1092)
                     /gene="hisE"
                     /locus_tag="VFL49_07120"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET7911784.1"
                     /translation="MSDSIARLYDAVIAARGADPAKSRTARLLRAGRAKMAKKLAEEA
                     VEVVIDAMHGQPEAVVMESADLIYNLVVLWAHAGVRPEDVWTEMRRRELMFGIAEKVP
                     KDLVRTAPRRKVVSLESRRARKRR"
     gene            complement(1268..4372)
                     /gene="putA"
                     /locus_tag="VFL49_07125"
     CDS             complement(1268..4372)
                     /gene="putA"
                     /locus_tag="VFL49_07125"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HET7911785.1"
                     /translation="MVPATAKRLERDIPPLRAPYAPPDEAIAAALLASAARPVDAEAR
                     IDARATRLVEAIRSRTGGLGGVEDFLHAYAMSTKEGLALMVLAEALLRVPDAETADRL
                     IEDKLQAGDWAHHGSRSAPLLVSASAWTLGLTVKVIQPGETPEGIVDALIKRLGLPAV
                     RAATRQAIRLLGSHFVMGQTIQEALGRAGSRREFLYSFDMLGEGARTAGDARHYFNLY
                     ARAIDAIAKTAGNDPLPRRPGISVKLSALHPRYEPLSRDRVLAELVPDLLELAQLAKR
                     HELNFTVDAEEADRLELSVDVIAAVLRDHSLAGWDGFGLAVQAYQKRATPLIGWVADA
                     AESLNRRLMVRLVKGAYWDTEVKRAQERGLPGYPVFTRKAMTDLCYLDCVRKLLAARP
                     RLYPQFATHNALTVACVIEDAGGTSGYEFQRLHGMGEALYESLLGELPDLSCRVYAPV
                     GGHADLLAYLVRRLLENGANSSFVSVAADPSVPVEAILRRPQSWIGDASHARHAHIPL
                     PCDLFGASRRNSAGVEFGDSAAFEALLAEVRAAPKIVDAKPLIDGAAAAGRGREVFSP
                     IDGGVIGVVHEGDESLVAAAVVSAQAAFPAWNATPVTERAAALDRAGGLIEQNRGRLI
                     ALLQNEGGKTIDDCVSEVREAVDYCRFYAQDALRTLAAQALPGPTGESNELRMRGRGV
                     FVCISPWNFPLAIFTGQIVAALAAGNGVVAKPAEQTPLVAFEVVKLLHRAGIPANVLN
                     LLPGDGKTGALLVADHRVAGVAFTGSTEVARSINRALAAKDGPIPSLIAETGGINAMI
                     VDATALPEQVTDDVIASAFRSAGQRCSALRLLCVQEDVAERVIDMVIGAARELKLGDP
                     RDLATHVGPVIDAEARSKLERWIAAMDSRGAVRFRLEASPPAGGTYVAPAIIEIDSAR
                     ELKEEVFGPVLHVVRWRSGELDRLLDDIAANNTALTLGIHSRIEAVVARIAARMPHGN
                     IYVNRNMIGAVVESQPFGGSHLSGTGPKAGGPNYLWRFALEQVVTVNTAAAGGNATLL
                     SEEE"
     gene            4593..5621
                     /locus_tag="VFL49_07130"
     CDS             4593..5621
                     /locus_tag="VFL49_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911786.1"
                     /translation="MRAHHTGWWAWLPLLIAATLTTSVAAGETIVKFSLDHGFQGPAA
                     LFLHPLDKGYFKSEGLNVTINPAVNSIEPVERVASGEYDIGFGDINTLIKYRDTKPGA
                     PVKAVFMLYNRPPFAVIGRKSRGIVVPRDLEGKTLGAPASDGSFLQWPIFVHVNGIDA
                     SKVKIENVSVPVREPMLAAGQIDAITGYSFAAYIDLKDKGVPVADISVLLMADYGVEL
                     YGSAIIVNSKFATEHPEAVRGFLNAFMRGLRETTRNPATAIDSVLRRNPDARKNVELE
                     RLTMAIRENIVTPEVKANGYGVIDEQRFARAIDQIAAVHKFKATKPKLDDVFDASFLP
                     TLNERRFN"
     gene            complement(5637..>6733)
                     /locus_tag="VFL49_07135"
     CDS             complement(5637..>6733)
                     /locus_tag="VFL49_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HET7911787.1"
                     /translation="IVKVAGEALADAGIAAKDVDEIILGHFNAGFSAQDFTASLVLQA
                     SNDLRFKRATRVENACATGSAAVHQGIKTIEARAARIVLAVGAEQMTVTPAPEIGRNL
                     LKASYVREEADIDGGFAGIFGKIAGLYFQKWGDQSDALAMIAAKNHKNGVGNPYAQMR
                     KDLGYDFCRTESEKNPFVAGPLKRTDCSLVSDGAAAVVLADVETALKLRKAVAFRAAE
                     HVQDFLPMSKRDILKFEGCAQAWQRALAQSGLQLGDLSFVETHDCFTIAELIEYEAMG
                     LTAEGQGARAVKEGWTQKNGKLPVNPSGGLKAKGHPIGATGVSMHALAAMQLTGAAGD
                     LQVKNARLGGIFNMGGTSVANYVSVLERIK"
BASE COUNT         1359 a         2181 c         2024 g         1169 t
ORIGIN      
        1 agggaaatca atcagcctga cggcacccgc ttacccgctt tcggtggcga aaatgaggaa
       61 ccggctccgt cagaacaagt atagcgcagc caccactccg atgacgagaa ccagggcgcc
      121 gagcagcacc cataggccca ggcgtctctc gatgatgtgc cgcgcactct cgccgtagcg
      181 gtgcaggaga aacgccagca ggaaaaaccg ggcgccgcgc gtaatgacgg aaaagaggat
      241 gaacagtccg aggttgtaac cggcaaagcc tgacgtaatg gtgacgatct tgtagggaat
      301 cggggtcagg ccctttaaaa ggataatcca ggcaccccac tccgcgtagg ctccgcgaaa
      361 ggcttcaacc ttgtccccat agccataaag ctgaatgagc caaagcccga tggaatcata
      421 aagaagcgcc ccgatggcat agccgagcac gcccccgagc accgacgtca cggtgcacca
      481 gatggccatc acataggcgc gctccggccg cgccaaagcc atcgggataa gcatcacgtc
      541 aggaggaacc ggaaagaacg agctttcagc aaacgaaacg gcccccataa gccacattgc
      601 gtagggctta taggctgcgg caatgcacca gtcataaaga cggcgcagca cggaacgcgg
      661 ctcggcggcc tcaacagtca tcagagcact cgtcgggaga attgtgacgg atcaacggcg
      721 cttgcgcgcg cgcctgctct ccagagacac gactttacgc cttggagccg tccggaccag
      781 atcttttggc actttttcgg caatgccgaa catcaactca cggcggcgca tttcggtcca
      841 gacatcctcc gggcgcacgc cggcgtgggc ccatagcacc acgagattgt agatcaggtc
      901 ggcgctttcc atgaccacgg cttcgggctg tccgtgcatc gcatcgatga cgacctcgac
      961 cgcctcttcg gcgagctttt tcgccatctt cgcacggccg gcgcgcagca gccgggcggt
     1021 tcgcgatttc gccgggtccg cgccgcgcgc cgcgatcacg gcgtcataaa gcctggcaat
     1081 cgaatcactc atacgcgcag gatacccgaa ccgcgcgcga aaccggttaa cgcatatgac
     1141 ggtcttcgcg gtcggggccc ggcattcgcg tcaccttatt tgaaggattg aggggaaaat
     1201 gggcccaaag cgaagcagtt cggcctcgaa cgcgccgttc gcgcactgtg acgccggcac
     1261 ccgggcgcta ttcttcttcc gacagcagcg tggcgttgcc tcccgccgcc gccgtgttta
     1321 ccgtcacgac ctgctcgagc gcaaagcgcc acagatagtt cggaccgccg gccttcggcc
     1381 cggtcccgga taaatgactc ccgccgaacg gctgtgactc gaccaccgcg ccgatcatgt
     1441 ttcggttcac atagatgttg ccgtgcggca tgcgcgcagc aatgcgcgcg acaaccgctt
     1501 cgatgcgcga atgaatgccg agggtcagcg cggtgttgtt tgcggcaata tcgtcgagca
     1561 gccggtccag ttcgcctgag cgccagcgca ccacatgaag aaccggcccg aagacctcct
     1621 ccttgagctc gcgggcactg tcgatctcga taatcgccgg ggcaacatag gtcccgccgg
     1681 ccgggggact tgcttccagg cgaaaccgga ctgcaccgcg tgaatccatc gcggcaatcc
     1741 agcgctcaag cttgctcctc gcctcggcgt cgatgacggg gccgacatga gtcgcgagat
     1801 cgcgcggatc gccgagctta agctcgcgcg cagcaccgat aaccatgtcg atcacgcgtt
     1861 cggcgacgtc ttcctgcacg catagcagcc gcaatgcaga gcagcgctgc ccggccgaac
     1921 ggaatgccga cgcgatgaca tcatccgtga cctgttcggg caacgcggtc gcatcgacga
     1981 tcatggcatt aatgccgccg gtttcggcga tcagcgacgg aatgggtcca tcctttgctg
     2041 ccagggcgcg gttgatgctg cgtgccactt ccgtcgagcc cgtgaaggca actccggcca
     2101 cccgatgatc ggcaacgaga agcgcaccgg tttttccgtc gcccggaagg agattgagga
     2161 cattggctgg aatgccggcg cgatgtagca gcttgacgac ctcgaaagcc acaagcggcg
     2221 tttgctcggc cggctttgcc acgacgccgt tgccggcagc gagcgcggcg acgatctgac
     2281 cggtgaaaat cgcgagcgga aaattccatg ggctgatgca gacgaagact ccgcggccgc
     2341 gcatgcgcaa ttcgttggat tcccctgtcg gacccggcaa tgcctgcgcc gctaacgtac
     2401 gcagcgcgtc ctgcgcgtag aaccggcaat agtcgacggc ctcgcgcacc tcggaaacac
     2461 aatcatcgat ggtcttgccg ccttcgttct gcagcagtgc gatgagccgc ccgcgattct
     2521 gctcgatcag accccccgcg cgatcgagcg ccgcggcacg ctccgtgacc ggcgttgcat
     2581 tccacgccgg aaatgcagcc tgcgcggata caacggcagc agcgacgaga gattcatccc
     2641 cttcatgcac caccccgatg acgccgccgt cgatgggcga aaagacctcg cgtccgcgtc
     2701 cggccgcagc cgcgccatca atcagtggtt ttgcgtcgac gattttcgga gctgcacgca
     2761 cctcggcaag caacgcctca aacgcggcac tgtccccgaa ttcgacgccg gctgaattcc
     2821 gccgcgacgc gccgaacaag tcgcagggca gcggaatatg cgcatggcgc gcgtgtgacg
     2881 cgtcgccgat ccagctctgc ggccgccgca ggatggcctc gaccggcacc gaaggatcgg
     2941 cagccaccga aacgaacgag gagttggccc cgttctccag cagccgtcgc accagatagg
     3001 cgagcaggtc ggcgtgtccg ccgaccggcg cgtagacgcg gcagctcaga tccggcaatt
     3061 ccccgagcag cgattcgtac agcgcctcgc ccatgccgtg cagccgctga aattcatagc
     3121 cgctggtgcc gcctgcatcc tcgatcacac aggcgacggt cagtgcattg tgagtcgcaa
     3181 attgcggata gagccgtggt cgtgccgcaa gcaacttgcg cacacagtcg agatagcaga
     3241 gatcggtcat cgccttgcgc gtgaacacgg ggtaaccggg aaggccgcgc tcctgtgcgc
     3301 gcttgacctc ggtatcccag taggcgccct tcacgagacg gaccatcaac cgtcggttca
     3361 ggctttctgc ggcgtccgcg acccagccga tcagaggcgt cgcgcgcttc tgataggcct
     3421 gcacagcgag accgaaacca tcccatcccg cgagcgagtg atcgcgcaac accgcggcga
     3481 tgacatcaac cgacaactca agtctgtccg cttcctcggc atcgaccgtg aaattaagtt
     3541 catggcgctt tgcgagctgc gccagttcga gcaaatccgg cacgagctcg gcaagcacgc
     3601 ggtcgcgcga caatggctcg tagcgcggat gcagcgcgga aagcttgacc gatattcccg
     3661 gacgccgcgg gagcgggtcg ttgcctgcag tcttggcgat cgcgtcaatc gcgcgggcat
     3721 ataagttgaa gtagtgcctg gcgtcgccgg ccgtgcgggc cccctcaccc agcatgtcga
     3781 aggaataaag aaattcgcgg cgcgaaccgg cgcgcccgag cgcctcctgg atcgtttgcc
     3841 ccatcacgaa atgcgaaccg agcaggcgta tcgcctgtcg tgtcgccgcc cgcaccgccg
     3901 gcaacccaag ccgcttgatc aaggcgtcga cgatgccttc aggggtttcg ccgggctgga
     3961 tgacctttac cgtaaggccg agcgtccacg ccgaagccga caccaggagc ggcgccgaac
     4021 gggagccatg gtgcgcccag tcgcccgcct gcaacttatc ctcgatcagg cggtcggcgg
     4081 tttccgcgtc gggcacgcgc agcagcgcct cggccagcac catgagcgcc aggccttcct
     4141 tggtcgacat cgcataagcg tgaagaaagt cttcgacgcc tccgagccca ccggttcggg
     4201 atcggatcgc ctcgacgaga cgggttgcgc gcgcgtcgat gcgtgcttcc gcatcgaccg
     4261 ggcgggccgc ggacgccaat aacgcggcgg caatcgcctc gtcgggcgga gcataaggtg
     4321 ccctgagcgg cggaatgtcg cgttcaagcc tttttgcggt cgccggcacc atcgtgaacc
     4381 ttcgaagcgt cccgcaattc tatctcaaag ccgccttgat cgtgccggag atttaggttg
     4441 ccggggagcg taaatggcgg cgttagtcat aacgcggcaa attggctgcc gaatatgtgt
     4501 gcgcgtattg ccctttgcag caacgtgtcc tttgctgtcc cgcaatcgac acagggccga
     4561 agcgtctcgg ccgctgaaga aggatgcgtg cgatgagagc tcaccacacc ggctggtggg
     4621 catggctgcc gctgctgatt gccgcgacgt tgacgaccag cgtcgcagcc ggggaaacaa
     4681 tcgtcaaatt ctcgctcgac cacggctttc aaggaccggc cgcgctcttc ctgcatccgc
     4741 tcgacaaggg ctatttcaag tcggaaggcc tcaacgtcac gatcaatccg gccgtcaatt
     4801 ccatcgagcc ggtcgagcgc gtggcatcgg gcgaatatga catcggcttc ggcgacatca
     4861 ataccctgat caaataccgc gacaccaagc ctggcgcacc ggtgaaggcg gtgttcatgc
     4921 tctacaaccg gccgccgttc gcggtgatcg gaagaaaaag ccgcggcatc gtcgtgccga
     4981 gagacctgga gggaaagacg ctcggcgcac cggcctccga cggatccttt ctgcaatggc
     5041 cgattttcgt gcacgtgaac ggcatcgacg cctccaaggt gaagatcgag aatgtgagtg
     5101 tcccggtgcg cgaaccaatg ctggccgcgg gccagatcga cgccataacc ggctactcgt
     5161 ttgcggcata catcgatcta aaggacaagg gcgtgccggt cgccgacatt tcggtactgc
     5221 tgatggcgga ttacggcgtc gaactttacg gcagcgctat catcgtgaat tcgaagtttg
     5281 caactgagca tcccgaagca gtgagaggct tcctcaacgc gttcatgcgc gggctgaggg
     5341 aaaccacaag gaacccggcc acggcgatcg actccgtatt gcggcgcaat cccgacgcgc
     5401 ggaaaaatgt cgagcttgag cggctgacga tggccattcg agaaaatatc gtcacgcccg
     5461 aagtcaaggc aaacggttat ggcgtgatcg acgaacaacg tttcgcgcgc gcgatcgacc
     5521 agattgcagc ggtgcacaag ttcaaagcga cgaaaccgaa actggacgac gtgttcgatg
     5581 catcgtttct gccgacgctg aacgaacgca ggttcaatta agtccggtcg tggcgcctat
     5641 ttgatccgct cgagaacact cacgtaattc gcgacggacg ttccgcccat attgaagatg
     5701 ccgccaagcc gcgcgttctt cacctgcagg tcgccggcgg cgccggtcag ctgcattgcc
     5761 gcaagcgcgt gcatggacac gcctgtcgct ccgatcggat ggcctttcgc tttcaggcca
     5821 ccggacggat tcaccggcag cttaccgttt ttttgtgtcc agccttcctt gaccgcgcgc
     5881 gccccctgcc cttcggccgt caaccccatg gcttcatatt cgatcagctc ggcgatcgtg
     5941 aagcagtcat gcgtctcgac gaacgataaa tcgccgagct gaagcccgga ttgcgcaagc
     6001 gcgcgctgcc acgcctgcgc gcaaccttcg aacttcagaa tgtcgcgctt cgacatcggc
     6061 aagaaatcct gcacatgctc ggcggcgcgg aacgcaactg ctttcctgag cttgagcgca
     6121 gtctccacgt cggccagcac gacggcggcg gcgccgtccg acaccagcga gcagtcggtc
     6181 cgcttcagcg gaccggccac gaacggattc ttctcactct cggtgcggca gaagtcgtag
     6241 ccgagatcct tgcgcatctg cgcatagggg ttgccgacgc cgttcttgtg attcttggcc
     6301 gcgatcatag caagggcgtc cgactgatcg ccccatttct gaaaatagag accggcgatc
     6361 ttgccgaaaa tgccggcaaa acccccgtcg atgtcagcct cctcgcgcac atacgacgct
     6421 ttcagcaggt tgcgtccgat ctccggcgcg ggagtcaccg tcatctgctc ggcaccgacg
     6481 gccagcacga tgcgcgcggc gcgcgcctcg atcgtcttga tgccctgatg cacggcggca
     6541 gaacccgtcg cgcaggcatt ttctacccgc gtcgcacgct tgaagcgcag gtcattcgag
     6601 gcctgcagga ccagggaagc ggtaaaatcc tgcgcggaga agccggcgtt aaaatgcccg
     6661 aggatgatct cgtcgacgtc cttggccgca atcccggcat cagccaacgc ctcgcccgca
     6721 accttcacga tca
//
LOCUS       DASSRL010000350         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1671347, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000350 DASSRL010000000
VERSION     DASSRL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671347"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..575)
                     /locus_tag="VFL49_07140"
                     /pseudo
     CDS             complement(1..575)
                     /locus_tag="VFL49_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964485.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            complement(796..1365)
                     /locus_tag="VFL49_07145"
     CDS             complement(796..1365)
                     /locus_tag="VFL49_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HET7911788.1"
                     /translation="MPDALELLKTRRSVKPMELVGPAPAPSEIETLLKIASRVPDHGK
                     LAPWRFIVFEGNARAAAGDAIAAAYRVDHPEATPEQIEFERKRLARAPLVIAVVSRAG
                     PHVKIPEWEQQLSAGASAMSLVFAAHASGYAASWITEWYAYDRRVLDALGIAPHERVA
                     GFVHIGRPAKPPEDRDRPKLDAIVTRFGT"
     gene            1478..2506
                     /locus_tag="VFL49_07150"
     CDS             1478..2506
                     /locus_tag="VFL49_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911789.1"
                     /translation="MDRAPATAAVCGRLWPALGLVLVAIALWPAHAQTPAQNSLFSAV
                     PSTGLISPLPSTRSEIFGPSSLPSAPPTPIPHAAPSTTASIPPVPAGQVALALSARFG
                     KDAQPIGGGLTWRVYNAKADTSGNFKLVKEDKSPAPTLVLPAGAYIVHVGFGLATAVK
                     PVDLRGSSVHEEFDLPAGGLRIEGKVGDVRIPAGQISFDVYKGSQFEAGDRRPIAEHV
                     MTSDVVVVPEGTYYIVSNYGDANSVVRSDIRVQAGKLTDITVTHRAAAITLKLVTDRG
                     GEALANTAWSVLTPGGDVIKESIGAFPRVILAEGEYRAIARNEGKVYEREFKVVTGVD
                     GDVEVLAR"
BASE COUNT          524 a          860 c          878 g          496 t
ORIGIN      
        1 cgaacaaatc cgagttcagg ttgaccgcaa tcaccagtcg cgcatcgagc gcgcgcggcc
       61 gaaacaggta ccggattgac cagggcgccg tcgaccagcc agcgtccgcc aatcaggacc
      121 ggcgggaaaa tgcccgggag cgaatatgac gcacgcaacg catcgctcag ccttccacgc
      181 gtgagccaga tctcgtgacc ggtgttgaat tccgtggcga ttgcggcaaa gcggatcgga
      241 aggtcttcca cgcggctttc cttcaggcct gcttcaagac gtgcggccag gtgcccgccg
      301 ccgatcaacc cggagcctga caggctgatg tcgagatagc cgagcacgcc gcgcacggtg
      361 agcgtgcgcg cccagctttc cagattgtcc atttcctttg cggcatagca tccgccgacg
      421 acggcaccga tggatgtgcc aacgatgacg tcgggcacga tgccgtgtgc ttccagggtg
      481 cggatcacgc cgatatgggc aaagccgcgg gcggcgccgc cgccaagtgc aaggccgatt
      541 gtcgggcgca ccacggccgg ctgcggatgc gtcaaggctt ccgccgcacg gtcgccattg
      601 cgcgctcgcg cgaataagcc ctcgaaaacc acgcgacgtt tctcctatac ccccgcgtct
      661 gaagggccgc gaatgcatgt atcggcccgg caggacaata gtgctggcgg agccttgtga
      721 ccgaaacgtg acagggaaac gtgcgcccgc actatttacg taatcgttaa atgccaaatg
      781 cgcgcgattg gttcctcagg tgccgaagcg cgtgacaatg gcatcgagct ttggccggtc
      841 acggtcctcc ggcggctttg ccgggcggcc aatgtgaacg aagcccgcaa cgcgttcatg
      901 cggagcaatg ccaagcgcat cgagaacgcg ccggtcatac gcataccatt cagtaatcca
      961 gcttgcggcg tagcccgagg catgggccgc aaagacgagg ctcatggcgg aggcgccggc
     1021 ggagagctgt tgctcccatt ccggaatctt cacgtgcgga cctgcgcggc tcacgaccgc
     1081 gatgacaagc ggcgcgcgcg cgagacgttt gcgctcgaat tcgatctgct cgggcgtggc
     1141 ctcggggtgg tcgacacgat acgcggcggc aattgcgtct ccggcggcgg cgcgtgcatt
     1201 accttcgaac acgataaatc gccaaggcgc cagcttgccg tgatccggca cgcgcgaagc
     1261 gattttcagc agcgtctcga tctcggacgg cgccggcgcc ggccccacca attccatcgg
     1321 cttcaccgag cggcgggttt tcagaagctc aagggcatca ggcattgtcg gcctttcgga
     1381 aattgcgccg ttgcgactag cacagcgtta acgaggtcac aaacaagcgg tcgagtggct
     1441 tgaaatcgcc gcgatttgag ccgaaaacaa cgatgtcatg gacagggcac ccgcaaccgc
     1501 cgccgtatgt ggccggctat ggccggcgct cgggcttgtt cttgttgcga ttgctttgtg
     1561 gcccgcccac gcgcagacgc ccgcacagaa ttcactgttc tcggcggtgc cgtcgacagg
     1621 gctgatctcg ccgttgccgt cgacgcggag tgagatcttc gggccgagca gtctcccgtc
     1681 ggcgccgccc acaccaattc cccatgcggc tccgtcgacc acggcgtcga ttcctccggt
     1741 cccggcaggg caggtcgcgc ttgcgctgag cgcacgcttt ggcaaggacg cacaaccgat
     1801 cggcggcggt ctgacgtggc gcgtctacaa tgcaaaagcc gatacaagcg gcaacttcaa
     1861 actcgtgaag gaagacaagt cgccggcgcc gacgctcgtg cttccggcgg gagcttacat
     1921 cgtgcatgta ggcttcggac tggcgactgc cgtcaagccg gtggatctcc gcggctcgag
     1981 cgtgcacgaa gagtttgacc tccctgcagg cggccttcgc atcgaaggca aggtcggcga
     2041 cgtgcgcatt cccgccgggc agatttcgtt tgacgtctac aagggcagcc agttcgaagc
     2101 cggcgaccgg cggcctatcg ccgagcacgt gatgaccagc gacgtcgtgg tcgtgcctga
     2161 gggcacttat tacattgtgt cgaactacgg tgatgccaat tcggtggtcc gctccgacat
     2221 tcgcgtgcaa gccggaaagc tcaccgatat caccgtcacg caccgcgctg ccgccatcac
     2281 gctgaaactg gtgaccgatc ggggcggcga agcgctggcc aacaccgcgt ggtcggtgct
     2341 tacgccgggc ggcgacgtga tcaaggaatc aatcggcgca tttccacgcg tcattttggc
     2401 cgagggtgaa tatcgcgcga tcgcccgcaa tgaagggaag gtctacgagc gcgaattcaa
     2461 ggtcgtgacc ggcgttgacg gcgacgtcga agtgctggcg cgctaggcac gctgcaccga
     2521 actttctgca tcggacggaa ttatcattgt gcaaaccatt gccaacgacc ccgtgatgga
     2581 cgttttttct ggcctggatt tgaaaaagat cgctctgctg ctcgatgtgg acggcacaat
     2641 cgtcgatatc ggcccgtcgc cgggtgaagt gcaggtttcc gacacattgc tccggtcgct
     2701 tgaacggctg cacgaactgt ccggcggcgc cattgcgctg gtgagcggcc ggcggatc
//
LOCUS       DASSRL010000351         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1514048, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000351 DASSRL010000000
VERSION     DASSRL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514048"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFL49_07155"
     CDS             complement(<1..641)
                     /locus_tag="VFL49_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET7911790.1"
                     /translation="MSTADKRRAFHALHQSGCFVIPNPWNVGSARYMQSLGFKALATT
                     SSGYAHSIGYADGDVTRDMMLAHYRELAQATDIPLNADFEGGYADDPDEVAANVRLCI
                     DTGVAGLSIEDFTGDESHPLYDFELAVRRVRAARDAIDKAGGDVVFTARTEGFIHNRP
                     DLDETIRRLKAFADAGADCLYSPGIKTREHIEATIKAVSPKPINFLNSGAFGYT"
     gene            886..1155
                     /locus_tag="VFL49_07160"
     CDS             886..1155
                     /locus_tag="VFL49_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911791.1"
                     /translation="MPSLSVKILVATFASVFVLATPANADKVRKHKKVAATSKTMQAD
                     AYHRGTNLVPAGPLYFNEVYLGDDPDPNIRFQLLRDISGRLGGDP"
     gene            1282..2463
                     /locus_tag="VFL49_07165"
     CDS             1282..2463
                     /locus_tag="VFL49_07165"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7911792.1"
                     /translation="MLHTIAAFDRIGEENAFAVLARANALAAQGRDIINLGIGQPDFR
                     TPDFIVEAAVKALRDGHHGYTPANGIPPLREAVAADLARRFQVEVSPDSVMIMPGGKP
                     TMFMSILMFGEPGAEIMYPDPGFPIYRSMIEYTGAKPIPVPIREENGFAFSAEETLKL
                     ITPRTRLLILNSPANPTGGVTPKSEVDKLVTGLEKWPDVAVMSDEIYDHMVYDGEKHV
                     CLLSYPSIRDRLILLNGWSKTYAMTGWRLGYAVWPGKLYDYARKLAVNLHSCVNASAQ
                     YAGLAALKGSQDEVTKMIAEFDKRRKVVVEGLNKLPGVSCATPKGAFYAFPNIKRTGW
                     KAKALASALLEDAGVAIIGGPDFGILGEGYIRVSYANSTANILKALDRMREFLANRKA
                     A"
BASE COUNT          623 a          917 c          944 g          665 t
ORIGIN      
        1 gtatagccga acgcgccgct gttgagaaag ttgatcggct tggggctgac ggctttgatc
       61 gtcgcctcaa tgtgttcgcg tgttttgatc ccgggcgaat acaggcaatc ggctccggca
      121 tcggcaaagg ccttcaggcg gcggattgtc tcgtcgagat ccggacgatt gtgaataaat
      181 ccttccgtgc gcgcggtgaa cacgacgtct ccgccggctt tatcgatcgc gtcgcgcgcg
      241 gctcgcacgc gcctgaccgc cagctcgaag tcgtaaagcg gatggctttc atcgccggtg
      301 aagtcctcga tcgacagtcc ggcaactccg gtatcgatgc acagccggac attggccgcg
      361 acctcatcgg gatcgtcggc atagccgccc tcgaaatcgg cgttcagcgg gatgtccgtt
      421 gcctgcgcga gctcgcgata gtgcgcgagc atcatgtccc gcgtaacgtc gccgtccgcg
      481 tagccgatcg agtgggcata acccgaactc gtggttgcca gcgccttgaa gcccaggctt
      541 tgcatataac gcgcgctgcc gacgttccac ggattgggaa tgacgaagca gccgctttga
      601 tgcagtgcgt ggaacgcgcg gcgtttgtcg gcggtactca tgattttgtc ctgcacaagg
      661 tgggacctgc acaatgcccc caacacgccc gcgattgcaa catgggtatc caccgttggt
      721 ccgaggccgc tgacaacggg caaacgcgaa caaaaatcat actaaacacc cgagccacgc
      781 gaatattccg acaccgactt gtgatcgact tgacgcgcca tttactttgc gcgcttgtcg
      841 caattcgtta agcggaaggc atcctgattt ctttcggagg ctgccatgcc gtctctctcc
      901 gtcaagattt tggtcgccac cttcgcctcg gtttttgtac tcgccacgcc ggcaaatgcc
      961 gacaaggtgc gcaagcacaa gaaggttgct gcgacatcca agaccatgca ggccgacgct
     1021 taccatcggg gaaccaatct cgtgccggca ggaccactgt atttcaacga ggtctatctc
     1081 ggtgacgatc ctgacccgaa tatccgcttt cagctgctgc gggatatcag cgggcgcctg
     1141 ggcggagacc cttgaaaggc ggtcgcgacg aaggtcgtgg cagcgtttga tttttgccga
     1201 ctgatcggct gaagtgcagt cctcattgag gggccggcgc gggcgtgtcc gcaaacggcg
     1261 cctgtgcggg aggactgcag aatgcttcat accatcgcgg ctttcgaccg catcggcgag
     1321 gaaaacgcct ttgcggtgct cgcccgggcc aacgcccttg cagcgcaggg ccgcgacatc
     1381 atcaacctcg gcatcggtca gccggatttc cgcacgccgg attttatcgt cgaggctgca
     1441 gtgaaggcgc tgcgcgacgg ccatcatggt tacacgcccg cgaacggcat tccgccgctg
     1501 cgggaagcgg tcgctgccga tcttgccagg cgtttccagg tcgaggtttc gcccgacagc
     1561 gtgatgatca tgcccggcgg caagccgacg atgttcatgt cgatcctgat gtttggcgag
     1621 ccgggcgcgg agatcatgta tcccgatccc ggatttccaa tctaccgctc gatgatcgaa
     1681 tataccggcg ccaagccaat tcctgtgccg atccgcgagg aaaacggttt tgcattttcc
     1741 gccgaggaga cgttaaagct gatcacgccg cgcacgcgcc tgttgatcct caattctccg
     1801 gccaatccga cgggcggcgt cacaccgaaa agcgaagtcg acaagctcgt gacaggcttg
     1861 gagaagtggc ccgacgttgc cgtcatgtcc gacgagatct acgaccacat ggtctacgac
     1921 ggcgagaagc acgtctgtct gctgtcctat ccgtcgatcc gcgaccggct gatcctgctc
     1981 aatggctggt cgaagactta cgcgatgacc ggctggcgcc ttggttatgc ggtctggccc
     2041 ggaaagctct acgactacgc gcgcaagctt gcagtcaatc tgcattcatg cgtcaacgcc
     2101 tcggcacagt atgctggcct tgcggcgctg aaaggctcac aggatgaagt gaccaagatg
     2161 atcgcggagt tcgacaagcg ccggaaagtg gtggtcgagg gactcaacaa attgcccggc
     2221 gtatcatgcg cgacgcccaa gggcgcgttc tatgctttcc ccaacatcaa gcgcaccggc
     2281 tggaaggcaa aggccttggc ctcggcgctg ctggaagatg cgggcgttgc catcatcggc
     2341 gggccggatt tcggcattct cggcgaaggc tatatccgcg tgagctatgc gaactcgacg
     2401 gcaaacatcc tgaaggcgct cgatcgcatg cgcgagttcc tggcaaaccg caaggccgcg
     2461 tgagctgggt gcaggtcaat accagtacgc cctatcccca gtgcgggact tttctggccg
     2521 gcaagcagct cgcgccgccg cccgatcacg tcttgaacat gctcaccagt ttgcgcctgg
     2581 agacgggcag aaatggaggt cacgagggct gtcgcgattg gtgcggagcg gatatcccag
     2641 aagcagggcc aatgtcgcga atgacgcaaa gcggacatcg ccaatcgata cgatttgaat
     2701 taatatttta agcacaccga aggcggcgct cgcgtactgg ttgttgtgac taccgggcgt
     2761 cgaggcttgg tgatgaccgt tatccgaaaa ctctttcgct ttatgtttgc cccgcttttc
     2821 gaaccccggt tctacgtgct ggctggggtc gtgatgtttt tgtgcttctc agttgcgctc
     2881 agtcaagatc ggttcacact tacatcggag aacacgaagc gggtcattac caggcaaaac
     2941 gagccaactc tttattgggg gacactagcc gcactcgcgt cgtttggcgt gttctgtttt
     3001 ggcgggggaa tttattttac gaggcggaca cggcgaggct gactcatgat cgacgcggtc
     3061 attgaaggtc tcatcggcgg ttatttgggc gaccgcctgc ttaagcttat accgccgaaa
     3121 aagaaatcgg cgttcgatgc gctttcatg
//
LOCUS       DASSRL010000352         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_184191, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000352 DASSRL010000000
VERSION     DASSRL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184191"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFL49_07170"
     CDS             complement(<1..734)
                     /locus_tag="VFL49_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET7911793.1"
                     /translation="MARADAASASAHPDSIKGLAQSIANPAYRRLLNAEPLLRRAVPV
                     LIIAFLVTICVGALVQVLDHRRQTLNSEILSLEAAADLAAERLHRPLKRPAAAIEQPQ
                     QVLAQALPSWAMAAGRRFILTNRDGVVAAATPATDATIGRRLIDLLGATQPLTTFGAG
                     AGVMEITLPDSSRALATVRMLNAPFGQLAVIQHRQAALSEWRSLTTLTITLSSTTGFV
                     VLILGFAFHWQATRAREADLIYETVR"
     gene            complement(910..>2352)
                     /locus_tag="VFL49_07175"
     CDS             complement(910..>2352)
                     /locus_tag="VFL49_07175"
                     /inference="COORDINATES: protein motif:HMM:NF036590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HET7911794.1"
                     /translation="KEKFRAGMDLYFARHDGEAATVEQFIRCFADASGRDMAQFMRWY
                     SQAGTPEVTVNGHFDAARKAFIVECRQNVPATPGQPTKEPMVIPLSIGLVGKNGRDLA
                     LKTGGGRPLEGQDIVLTEQTARFEFGDIGERPVLSINRGFSAPINLQTDLGIDDLAFL
                     AAHDSDAFNRWQALQTISMRLLLDNVAAARAGRPLRTDDRLVQAFAATIEDSNLEAAF
                     VALALILPGEGDIAREIGNDIDPDAIFQTRTALKAELGQRLDARLADAYERMTVGVRY
                     SPDAESAGRRALRNAALDLLAASAAPAGIARAVRQYQHADNMTDRMAALATLSLHNVP
                     EREHALADFYDRYAGDALVVDKWFSLQATTPHRDTLDHVRVLTTHPAFSLANPNRVRA
                     LIGAFAQGNSTQFNRLDGAGYDFIAETVLALDQKNPQVSARLATAFRNWRTMEDGRQV
                     KAESALSRIRAASPLSRDLSDIVDRALAAP"
BASE COUNT          471 a          681 c          748 g          452 t
ORIGIN      
        1 ctgcggaccg tttcgtagat gaggtcagct tcccgcgccc gcgtcgcttg ccaatggaat
       61 gcgaatccga ggatgaggac gacaaagccg gttgtggagg agagggtgat cgtcagggtt
      121 gtcagtgacc gccattcgga aagcgcggcc tggcgatgct gtatcaccgc aagctggccg
      181 aacggggcat tgagcatgcg aaccgttgcc agcgcccggg agctgtctgg caacgtaatt
      241 tccataactc ctgcgccggc accgaaagtc gtcagaggct gggtagcgcc caacaggtcg
      301 atcagccgtc ggccaatcgt ggcgtcagta gcgggcgtgg cggcagcaac cacgccatca
      361 cgatttgtca ggataaagcg gcgtcccgcg gccatggccc aggacggcag agcctgcgca
      421 agcacctgtt gcggctgctc gatggcggcg gccggccttt tcagcggacg atgcagccgc
      481 tcggcagcaa gatcggccgc ggcttcgagt gacaggattt cgctgttgag ggtttgccgg
      541 cggtgatcga gcacctgcac gagggctccg acgcaaatgg ttacaagaaa tgcgatgatc
      601 aggacgggga ctgcgcggcg caggagcggc tcagcattga gcagccgtcg atacgccggg
      661 ttggcgatgg attgcgcgag gcccttgatg gaatcgggat gtgcagacgc actcgccgca
      721 tcggcgcgcg ccataatcgg cctccgttgg aagttcccct gtcacccacg aaacggaagg
      781 atgtgccgca tcgcttcccg aatcacttgc cgattggaat cgatagatgg tgatttgtcg
      841 agagtcggaa gagtcaaatg tttaaaaaaa gttacccacg gatagtttct gcaacacaaa
      901 ccaaccgatt cacggtgcgg ccaacgctcg atcgacgata tctgaaaggt cgcgcgacag
      961 aggggacgct gccctgatcc ggctcagggc gctttcggcc ttcacttgtc ggccatcctc
     1021 catcgtgcgc cagttgcgga atgccgttgc gagccgcgcc gaaacctgcg gatttttctg
     1081 gtcgagcgcg agcacggttt cagcgatgaa atcatacccc gctccatcaa ggcggttgaa
     1141 ttgcgttgaa ttgccctgtg cgaacgcccc gatgagcgcg cgcacgcggt tgggattggc
     1201 gagcgagaag gctggatggg tggtgagaac gcgaacgtgg tcaagcgtgt ctcgatgcgg
     1261 ggtcgtcgcc tgcaacgaga accacttgtc gaccaccagc gcatcgcccg cgtaccgatc
     1321 gtagaaatcg gccaaagcgt gctcgcgttc tggcacatta tgcaaggaga gggtcgcgag
     1381 agccgccata cgatcggtca tgttatcggc gtgctgatat tgtcggacgg cgcgcgcaat
     1441 tcctgcgggt gcggcgctcg ccgccaggag gtcgagggcc gcattccgta gggcgcgtcg
     1501 cccagcgctc tcagcatcag gactgtagcg caccccaaca gtcattcgct cataagcgtc
     1561 ggccagccgg gcatcaagac gctgaccgag ttctgccttc aaggcagttc gcgtctggaa
     1621 gatggcgtca gggtcaatat cgttcccgat ctcgcgcgcg atgtcgcctt caccgggcaa
     1681 aattagcgcc agtgccacaa atgcggcttc caagttgctg tcctcaatcg tggcggcgaa
     1741 cgcctgcacg agccggtcat cagtgcgaag gggacgcccg gcacgcgcgg cggcgacgtt
     1801 atcgagaagc aggcgcattg agatggtctg gagcgcttgc caacgattga atgcgtcgct
     1861 gtcgtgcgcc gccaggaaag cgagatcgtc gatgccgaga tctgtctgca ggttgattgg
     1921 agccgaaaag ccgcgattga tcgaaaggac aggacgttct ccgatgtcgc caaattcaaa
     1981 tctggctgtt tgctcggtca gcacaatgtc ctgaccttcg agaggccttc caccgcctgt
     2041 tttgagcgca aggtcgcggc cattttttcc gacaagcccg atcgaaagcg gaatcaccat
     2101 cggttctttg gtcggctgcc cgggagtggc cggaacgttc tgcctgcatt ccacgatgaa
     2161 agccttgcgg gccgcatcaa agtgtccgtt gaccgtcacc tccggggtgc ctgcctggct
     2221 gtaccacctc atgaactgag ccatgtcacg gccactggca tccgcaaagc accggatgaa
     2281 ttgctcaacc gtcgcggcct cgccgtcatg gcgggcaaaa taaaggtcca taccggcgcg
     2341 gaatttctct tt
//
LOCUS       DASSRL010000353         4971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_236908, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000353 DASSRL010000000
VERSION     DASSRL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236908"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFL49_07180"
     CDS             complement(<1..294)
                     /locus_tag="VFL49_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET7911795.1"
                     /translation="MGGLAAALLPAVKKSGVVWFGSSGRTKDIATGGAPPFVQIESYG
                     HGSIATVDVPEQHYTGFYEGFSNSALWPVLHSRIDLSRPRPEDYGSYRAINNYM"
     gene            complement(454..3195)
                     /gene="treY"
                     /locus_tag="VFL49_07185"
     CDS             complement(454..3195)
                     /gene="treY"
                     /locus_tag="VFL49_07185"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET7911796.1"
                     /translation="MMAPAVPLATYRIQLTKDFGFEQTAALIPYLAGLGISHLYASPF
                     LKARPGSTHGYDIVDHDRLNAELGGDAGFIRMSAALREHGLGLILDFVPNHMGVGRAD
                     NAWWLDVLEWGQRSPYADAFDIDWNALPHRRHPGILLPILGRPYGEVLEAGEIELKFD
                     ATGGGFAAWYFEHKLPINPQRYAEIIRTVVAHAGETSTPASHALLALADEYRNPSAPS
                     YREAPALKKALAAINGADELIARGLAAYRSGGAALLHRLLERQHYRVAYWRVAISAVN
                     YRRFFDINDLAGIRVENPATFRAIHTLISRLIATGQLQGLRLDHIDGLRDPSQYTKRL
                     HQLVRKTRREAGLPPAFYIVVEKILGEGERMPNLPGVAGTTGYEWLNVLTHLQLNGTG
                     LQQLDATWRDFTGEQRDFPVILENAKRRVIDTILASEFGVLCGALSRIAAGHFSTRDF
                     TIDRLRTALRLYVLEFPVYRTYVTAAGASDRDRKLIGDVISRARARWEGPDPQIFAFL
                     HDAVTLDLAGDAGYSAPRVRDFALKLQQFTGPLMAKSMEDTAFYRFGRLLALNEVGGD
                     PASGELPVASFHELQRQRIESGQGGLTATATHDTKRGEDARARILAISEIAADWDAAV
                     KDWHSRNAAHVRHSNGRRLPTRAHEYMIYQSLVGVWPGAVSADLVNRVQAYAIKAARE
                     GKQETSWTDPDEAYETALSRFVARLLDPQISAGFLASLETFAQRTSLLGALNSLSQLA
                     LKATLPGVPDFYQGTELWDLSLVDPDNRRPVDFSRRSDLLRAHETDWAKLAADWRSGE
                     IKFELTRSLLRLRCEWPNLFQQGNHEPLAATGAHGGNVIAFSRAWKNQHVVVAVARQF
                     GPVTNGGRVWPQGWNARLNLPVGTYEQVLHPRSDVCTGAVAISDVFAWLPVSVLLSR"
     gene            complement(3192..4943)
                     /gene="treZ"
                     /locus_tag="VFL49_07190"
     CDS             complement(3192..4943)
                     /gene="treZ"
                     /locus_tag="VFL49_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET7911797.1"
                     /translation="MSALSQFGPLVSDRGVTFRLWAPAARIVHLMLDHKMPMPQKDGW
                     FVLQIEEAAPGARYAFEIDEDITIPDPASHFQPEDVHGPSEVIGHDFAWQCGQWQGLP
                     WQETVFSELHVGTFTKDGTFRAAISKLDHLARTGITAIELMPLADFPGRWNWGYDGVL
                     PFAPDSSYGRPDDLKTLIDAAHARGLMIFLDVVYNHFGPEGNYLGRIAPQFFTTAQTP
                     WGSAIDYRVPEVRRFIIENALHWLRDYKFDGLRLDAVHAIVRPGDPSILHDLSFAAGS
                     LAKETGRHIHLVLENDDNQARFLAPGDDPPAGRYRAQWNDDYHHAWHVLLTGERAGYY
                     VDYGGTCHIERTLTEGFAYQGEKSEHRKGQPRGQASSHLPPTAFVNFIQNHDQIGNRP
                     LGERLTTLCPPEALAAALAVLLLQPSPPLLFMGEEWGATHPFPFFCDFEGELADAVRN
                     GRKKEFAEVYAQHGDEIPDPLSADTRASAVLDWTVMSKPMHAERLALTRALLAVRKRA
                     VIPLLTSMHGDAEARITNSILFARWPAGARTLQLLANLADDPAPRPVLAWGDAIWGGL
                     PPPTMPPWSVYAAVKRR"
BASE COUNT          966 a         1509 c         1563 g          933 t
ORIGIN      
        1 catataattg ttaatggcgc ggtatgatcc gtaatcctcg gggcggggac gcgaaagatc
       61 gatgcgcgaa tgcagtaccg gccacaaagc cgaattcgaa aagccttcgt aaaagccggt
      121 gtaatgctgc tcggggacgt cgaccgttgc gatcgagccg tggccgtagg attcaatctg
      181 gacgaatggc ggggcgccgc cggtcgcaat gtccttcgtg cggccgctgg agccgaacca
      241 cacgacaccg gatttcttca ccgcgggaag cagggcggcc gccagtccgc ccattatggc
      301 tcgttcgggc gctgccgggg ccacccgatt tgatacgaca acgtccacgc tccgacccca
      361 ctcacgagca ctccccaaat cccagtaagg aaaaacgccg actcgatgtg aaagttccgg
      421 tgcggagtgt gcgccggcga tgcgctgata aaattacctg ctcagcaaga cgctcacagg
      481 aagccacgcg aaaacgtccg agatcgcgac cgcacccgtg cagacatcgc ttcgcgggtg
      541 caacacctgc tcgtaagttc cgaccggaag atttagcctc gcattccagc cctgcggcca
      601 gacgcgtccg ccgttcgtga ccggtccgaa ctgtcttgca accgcaacaa cgacatgttg
      661 gttcttccac gctcgcgaaa aggcgatgac gttgccgcca tgggcaccgg tcgccgcgag
      721 cggctcatgg tttccttgct ggaacaggtt tggccattcg caccgcaacc tcagcagact
      781 gcgggtaagc tcgaacttga tctcgccgct gcgccagtcg gctgccagct tggcccagtc
      841 ggtttcgtgg gctcgcagaa ggtcgctgcg tctggaaaag tccaccggcc gccggttgtc
      901 gggatcgact agcgacaggt cccaaagctc ggttccctgg taaaaatccg gtacgcccgg
      961 cagcgtcgcc ttgagcgcta gctgcgacag ggaattcaat gcacccagca ggcttgttcg
     1021 ctgggcgaat gtctcgagcg atgccagaaa tccggccgag atttgcggat caagcagccg
     1081 cgctacaaag cggctgagcg ccgtctcgta ggcttcgtca gggtcggtcc agctggtttc
     1141 ctgctttcct tcgcgtgcgg ccttgattgc gtaggcctga acccggttga caagatcggc
     1201 gctgacagcc ccgggccaga cgccgacgag gctctgatag atcatgtatt cgtgcgccct
     1261 tgtcggcagg cggcgcccgt tcgaatgccg gacgtgcgcg gcgttgcggc tgtgccaatc
     1321 cttgaccgca gcatcccagt ccgccgcgat ctccgaaatg gccaggatgc gtgcgcgtgc
     1381 gtcttccccg cgtttggtgt cgtgggtcgc cgtggcggtc agtccgccct ggccggactc
     1441 gatgcgttgc cgctgcagct cgtgaaaact cgccaccggc aactcgcctg aggccggatc
     1501 gccgccgact tcgttgagcg cgagcagccg gccaaaacgg tagaacgccg tgtcttccat
     1561 cgacttggcc atcagcggtc cggtgaactg ctggagcttc agcgcaaaat cgcgcacgcg
     1621 cggcgcgctg tagccggcat cgcccgcgag atcgagcgtg acagcgtcgt gcaggaaagc
     1681 gaaaatctgc ggatcgggcc cttcccaacg ggcgcgtgcg cgagaaatca catccccgat
     1741 cagctttcga tcgcgatccg atgcgcctgc cgcggtgacg taggtgcgat agacgggaaa
     1801 ctcgagcacg tagaggcgca gcgctgtgcg caggcgatcg atcgtgaaat cacgcgtgct
     1861 gaaatggccg gccgcgatgc gtgagagcgc gccgcacagc acgccgaact cgctggcgag
     1921 tatggtgtcg atgacgcgtc gcttggcgtt ctccagaatg accggaaaat cgcgctgctc
     1981 gccggtgaaa tcgcgccagg tcgcatcgag ctgctgtagg cccgttccat tcagttgaag
     2041 atgcgtcagc acgttgagcc attcgtagcc ggtcgtaccg gccacgccgg gaaggttggg
     2101 catcctttcg ccttcaccca gaattttctc gacgacgatg taaaatgcgg gtggcagccc
     2161 cgcctcgcga cgggtcttgc gcacaagttg atgcaaccgt tttgtgtatt ggctcgggtc
     2221 gcgcaggccg tcgatatgat cgagccgcaa cccctgcagt tgtccggtcg cgatcaatcg
     2281 cgagatcaaa gtatgaatgg cccgaaacgt cgccgggttt tccacgcgaa ttccggcaag
     2341 gtcgttgatg tcgaagaagc gccggtaatt gaccgccgaa attgcgacgc gccaatatgc
     2401 gacccgataa tgctgacgct cgagcaagcg gtgcagaagc gccgcaccgc cgctgcgata
     2461 ggcggcaagc ccgcgcgcga tgagctcatc cgccccgtta attgcagcca gtgctttttt
     2521 taatgccggc gcttcgcggt acgatggcgc gctggggttg cgatattcgt ctgcaagcgc
     2581 gaggagagcg tggctcgcgg gcgtacttgt ctcgccggcg tgcgccacga ctgtgcggat
     2641 gatttccgca tagcgctgcg ggttgatcgg cagcttgtgt tcgaagtacc acgccgcgaa
     2701 gccgccgcct gttgcgtcga acttcagctc gatttcgcca gcctcaagta cctcgccata
     2761 aggccgcccg aggatcggaa gcaggatgcc agggtgacgc cgatgcggca gcgcattcca
     2821 gtcgatgtcg aaggcgtcgg cataaggcga cctttggccc cactcaagga cgtcgagcca
     2881 ccacgcattg tcggcgcggc cgacgcccat gtggtttggc acgaagtcga ggataagtcc
     2941 gaggccgtgc tcccgaagcg cggcggacat acgtataaat cccgcgtcac cgccgagctc
     3001 ggcattgagc cggtcgtggt cgacaatatc gtaaccgtgc gtgcttcccg gccgtgcctt
     3061 caggaacggc gaagcataca gatgcgaaat gccgagccct gcgagatagg gaatgagagc
     3121 cgccgtctgc tcaaagccga aatccttggt caattggatc cggtaggtcg cgagcgggac
     3181 cgctggagcc atcatcgcct cttgactgcg gcatagaccg accacggcgg cattgtcgga
     3241 gggggaagcc ctccccagat tgcatccccc caggcgagaa caggtcgggg cgccgggtca
     3301 tccgccagat tggcaagcag ttgcaacgtt ctggctccgg ccggccaacg cgcgaaaaga
     3361 atggagttgg tgatgcgggc ctcggcgtct ccatgcattg acgtcagcaa aggaatgaca
     3421 gcgcgtttgc gcacggcgag aagcgcgcgt gtcaaagcga gccgttctgc atgcatcggc
     3481 ttgctcatga cagtccaatc gagcactgcg cttgcacgcg tgtcggcgga cagcggatcc
     3541 ggaatttcat cgccgtgttg cgcatagact tcggcgaatt cctttttgcg cccgttgcgc
     3601 acggcatccg ccaattcgcc ctcgaagtcg cagaaaaacg gaaatgggtg ggtggccccc
     3661 cattcctcgc ccatgaacag aagcggcggt gatggctgca gcagcagaac agcaagggcc
     3721 gccgccaacg cttcgggcgg acacagcgtc gtcagccgct cgccgagcgg ccggttgccg
     3781 atctggtcgt gattttgaat gaaattgacg aaagccgtcg ggggcagatg gctgctcgct
     3841 tgcccgcgag gctgtccttt ccggtgctcc gatttttcgc cttggtaggc aaagccttca
     3901 gtcaatgttc gctcgatatg gcacgtgccg ccatagtcga cgtagtagcc cgctctttcg
     3961 ccggtcaaaa gaacgtgcca ggcgtgatga tagtcgtcgt tccactgtgc ccggtagcgg
     4021 ccggctgggg gatcgtcgcc cggtgcgaga aagcgcgcct ggttgtcgtc gttttccagg
     4081 acgaggtgga tatgacggcc ggtctccttc gcaagtgatc cggccgcgaa actgagatcg
     4141 tgcaggatcg acgggtcgcc gggccttacg atcgcgtgca ccgcatcgag tcgcaagcca
     4201 tcgaacttgt agtcgcgcaa ccagtgcagc gcattctcga tgatgaaccg ccgcacctcc
     4261 ggcacgcgat agtcgattgc actgccccat ggcgtctgcg cggtggtgaa gaactgcggc
     4321 gcaatgcggc cgagataatt accctccggt ccgaagtgat tgtagacgac atcaaggaag
     4381 atcataaggc cgcgggcatg cgcggcatcg atcagcgtct tgagatcgtc cgggcggccg
     4441 tagcttgagt cgggggcaaa aggcagaacc ccgtcataac cccagttcca gcgtcccgga
     4501 aaatccgcga ggggcatgag ttcgatggca gtgattccgg ttcgcgccag gtgatcgagc
     4561 tttgatatgg ccgcacggaa cgttccatcc ttagtgaagg tgccgacatg caattcggag
     4621 aaaacagtct cttgccaagg caatccttgc cattggccgc actgccacgc gaagtcgtga
     4681 ccaatcacct cgctcggccc atgcacgtcc tcgggttgga agtgcgaagc aggatcgggg
     4741 atcgtgatgt cttcgtcgat ctcgaaagcg taacgcgcgc ctggcgctgc ctcctcgatc
     4801 tgcaacacga accagccgtc cttctgcggc atcggcatct tgtgatcgag catgagatgg
     4861 acgatgcggg cggccggtgc ccaaagccga aatgtaacgc cgcgatcgga caccaatggg
     4921 ccgaattgcg aaagtgcact cacgattcgc ctgcaaacgc gagaacggac c
//
LOCUS       DASSRL010000354         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1251662, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000354 DASSRL010000000
VERSION     DASSRL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251662"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFL49_07195"
     CDS             complement(<1..387)
                     /locus_tag="VFL49_07195"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HET7911798.1"
                     /translation="MMHATASLQSRRRTGRRYTILLFVVLAVIAGWTGFWKFAAGKAQ
                     QTIDGWRAREAQSGRIYNCGSQDVGGFPFRIEVTCDQASAVFRSNEPPIELKAGSVLV
                     VAQVYQPGLIIGEFHGPLIIGQLGKEP"
     gene            498..1067
                     /locus_tag="VFL49_07200"
     CDS             498..1067
                     /locus_tag="VFL49_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET7911799.1"
                     /translation="MKTGPDQSNEDLWVFAYGSLMWRPGFDFIERRNARLVGAHRALC
                     VYSFVHRGTPAKPGLVFGLDRGGNCRGIAYRIAARKRIKTLEYLRGREQVTLVYREAW
                     RRVWLDDDPQQSVHALCYVVDRGHRQYAGRLSLPEQLHFARQGHGRSGACRDYVLSAM
                     KELQALEIRDHDLQLLAEQLKGVHESAGR"
     gene            complement(1064..1798)
                     /locus_tag="VFL49_07205"
     CDS             complement(1064..1798)
                     /locus_tag="VFL49_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7911800.1"
                     /translation="MIILLRSLAFNVLFYVNTLLYLIAALPTFFMPYRAIIAVAKSWA
                     RANLVLLRVVAGIDYEIRGLEKMPSGPLIIASKHQSAWETFALLPLVSNPTFILKREL
                     QWIPIFGWLTIKGRMVPVDRGGGSHALAAMIERARIELADNRQLIIFPEGTRRPAGAE
                     PRYKLGVAHLYIAEGVPCVPIALNSGLFWPRRSLRRLPGTVVAEILDPIPPGLDKDAF
                     ASRLQNDLETATARLVAEGRAKMRAS"
     gene            complement(1810..>2243)
                     /locus_tag="VFL49_07210"
     CDS             complement(1810..>2243)
                     /locus_tag="VFL49_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET7911801.1"
                     /translation="DAIELLATDHGKRLLITGVHRATSAREIARLTPLYSKYFTCCID
                     LDRSALNTFGNALEAKRWARQHNFNSLIVVTSNWHMPRAMAELSHQLPDVELVAFPVI
                     SEKVKTEPWWSNLDTARLLFAEYLKYVFALARMRLDVDGAV"
BASE COUNT          454 a          666 c          704 g          419 t
ORIGIN      
        1 cggttctttt cccagttggc cgatgatcag cggtccatgg aattcgccga tgatcaggcc
       61 gggctgatag acttgcgcca cgaccaggac gctgccggcc ttgagctcga tcggcggctc
      121 attgctccgg aaaaccgcgg acgcctgatc gcaggtgact tcaatgcgga acggaaagcc
      181 gcccacatcc tgcgatccgc agttatagat ccgacccgat tgtgcctcgc gggcgcgcca
      241 cccgtcgatc gtctgctgcg ccttgccggc ggcgaatttc cagaaccccg tccacccggc
      301 gatcaccgcc aggaccacga agagcaaaat cgtgtaacgc cgcccggtac ggcgccggga
      361 ctgcagactt gctgtggcgt gcatcatctg tcttcaaaac ccgccgaatg cggcctttct
      421 gcgcccgacg gctgcaggtt gcgcgggaac cggggccctg ttacgcggac gtcggcagcc
      481 gaccgtcccc catcacgatg aagacaggcc ccgatcaaag caacgaggac ttgtgggtct
      541 ttgcctacgg ctcgctgatg tggcggcccg gattcgattt tatcgagcgg cgcaatgcgc
      601 gactggtcgg ggcacatcgc gccctctgcg tctactcgtt tgtgcatcgc gggacgccgg
      661 caaaacccgg actggttttt ggtctcgatc gtggaggaaa ctgccgcggc atcgcatatc
      721 gcattgccgc gcgtaagcgg ataaagaccc tcgaatacct gcgcggccgc gagcaggtca
      781 cgctcgtcta ccgtgaagcg tggcgcaggg tctggcttga tgacgatccg cagcagagcg
      841 tgcatgcttt atgttacgtc gtcgaccgcg gacaccgtca atacgcggga cggctgagcc
      901 tgccggagca gctgcatttt gcgcgccagg ggcatgggcg atccggcgcc tgccgcgatt
      961 atgtactctc cgcaatgaag gaactgcagg cattggaaat tcgtgatcat gaccttcagc
     1021 tgttggccga gcagctcaag ggggtccatg aatcggcggg ccgctaactt gcccgcattt
     1081 tagcccgccc ttccgcgacc agcctggcgg tcgcagtctc aaggtcgttc tgcagacggg
     1141 aggcgaaagc gtccttgtcg agcccgggcg ggatcggatc gaggatttca gcaacgacgg
     1201 tcccgggaag gcgacgaagc gagcgccgcg gccagaacag gcccgagttg agcgcgatcg
     1261 gaacgcaggg cactccttcc gcgatgtaaa gatgcgctac gccgagcttg tagcgcggct
     1321 cggcgcctgc cggacggcgc gtcccttcgg gaaaaatgat gagctgacgg ttgtcggcga
     1381 gctcgatgcg cgcccgctcg atcatggctg ccagcgcgtg tgaaccgccg ccgcggtcga
     1441 ccggcaccat gcggcccttg atcgtcagcc agccgaagat ggggatccat tgcagctcgc
     1501 gcttgaggat gaaagtcgga ttgctgacca gcggaagcag tgcaaaggtt tcccaggcgg
     1561 attggtgttt cgaggcgatg atgagcgggc ccgaaggcat cttttcgagg ccgcgaattt
     1621 cgtaatcaat cccggcaacg acacgcagca gcacgagatt ggcgcgcgcc caggattttg
     1681 cgaccgcgat gatcgcgcga tacggcatga agaacgtcgg gagcgccgca atcaaataca
     1741 gcaacgtgtt cacatagaac aacacgttga atgcaagcga gcggagaaga atgatcacgg
     1801 ccagcctttt tatacagcgc cgtcgacatc aagacgcatt cgagcaagcg caaaaacata
     1861 cttcaagtat tccgcaaaca gcaggcgtgc ggtatccagg ttcgaccacc aaggctcggt
     1921 tttcactttt tcggatatga cgggaaaagc gaccaactct acatccggca gctgatggct
     1981 gagttctgcc atggcgcgcg gcatatgcca gttagaggtc acgacaatca gcgaattgaa
     2041 gttgtgctgg cgcgcccagc gtttcgcttc gagcgcattg ccgaacgtgt tgagggccga
     2101 ccgatcaagg tcgatgcagc aggtaaaata cttggaataa agcggtgtca gtcgcgcgat
     2161 ttcccgcgcg ctggtggcgc gatgcacgcc ggttatcagc aatcgtttgc cgtgatcggt
     2221 ggccagcaac tcgatcgcat cgg
//
LOCUS       DASSRL010000355         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_499085, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000355 DASSRL010000000
VERSION     DASSRL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499085"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..635
                     /locus_tag="VFL49_07215"
     CDS             18..635
                     /locus_tag="VFL49_07215"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7911802.1"
                     /translation="MRAEQDRYRALARSGQSPEVMVIGCCDSRCSPEVIFNARPGELF
                     VVRNIANLVPPYAPDAHAHGVSAALEFGVMALKVKHIVVLGHAQCGGIKAFAEDAEPL
                     TPGDFIGNWMKLITPAAKKLGPRGSRPMDEYLTLLEQANVVLSLDNLMTFPRLRTMVE
                     RGDIRLHGAYFGVATGQLSVRDPATGKFTQVAADEYAQLFAHPRF"
     gene            809..1966
                     /locus_tag="VFL49_07220"
     CDS             809..1966
                     /locus_tag="VFL49_07220"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET7911803.1"
                     /translation="MLEERSIIAEIEGTFASGSADKQADILRRVTDLFLAGADNYSDD
                     HVDLFDGVISRIAEKIETKARAELAHRLAPAPNAPPMTVRKLAQDESIEVAGPILSLS
                     NRLSDEDLLAIASGKSQDRLLAISKRPSLSEKVSDVLVTHGNRDVVLSVTRNEGARIS
                     DAGYTKLVDRSINDEVLAICVSMRKDIPQEHFNALIAKASEVVFEKLAASNPHAVYEV
                     HKVLTDITGQEPAGQPKAKRDYREAAAQFDVVRRSGTPVDPVVCEYAKNGQFEETVAA
                     LSALSGAPIKLVESVMNDARPDSDFALILAKAAGLSWPTVKQICILRRKVFRFSPQAI
                     ENARHSFDRLQPKTAQRLVRFYNERHAALANFQELAQHIRAQESPARALDA"
     gene            complement(1988..>2695)
                     /locus_tag="VFL49_07225"
     CDS             complement(1988..>2695)
                     /locus_tag="VFL49_07225"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7911804.1"
                     /translation="EVNADAIAGFTRKNIIANPNCSTAQLVVALKPLHDKAKIKRVVV
                     ATYQSVSGAGKDAMDELFSQSKAVFTLDEIEAKKFPKRIAFNVIPHIDLFMDDGYTKE
                     EWKMVVETKKILDPKIRLSATCVRVPVFVGHSEAVNIEFEYPISADEAREILRNAPGC
                     LVIDKREDGGYVTPYECVGEDATYISRIREDGTIENGLQMWVVSDNLRKGAALNAVQI
                     AECLINRKLIQAKRRAA"
BASE COUNT          517 a          849 c          794 g          535 t
ORIGIN      
        1 cgtttcttgc gggcggcttg cgcgccgagc aggaccgcta tcgtgcgctt gcgcgcagcg
       61 gacaatcccc ggaagtcatg gtgatcggct gctgcgattc ccgctgctcg ccggaagtca
      121 ttttcaacgc ccgccccggc gagcttttcg tcgtccgcaa tatcgcaaac ctggtccctc
      181 cttatgcacc ggacgcgcac gcacatggcg tctccgccgc gctggagttc ggtgtcatgg
      241 cgctcaaggt caagcacatc gtcgtgctgg gccatgcgca gtgcggcggc atcaaggcgt
      301 tcgccgaaga tgccgagccg ctgacgcccg gcgatttcat cggcaactgg atgaagctga
      361 taaccccggc ggcgaaaaag ctgggcccgc gcgggagccg cccgatggac gagtatctca
      421 ctctgctgga gcaggccaat gtcgtactca gcctcgacaa cctgatgacc tttccccgct
      481 tgcggacaat ggtcgagcgc ggcgacatca gattgcacgg cgcctatttc ggggtggcca
      541 ccggccagct ttcggtgcgc gacccggcaa ccggaaaatt cacgcaggtg gcggccgacg
      601 agtacgcgca gcttttcgct catccgcgct tttgaccgcc aggcggcggc ttactcggcc
      661 ttaaccctga tcccccgaat tctccttgga aatcgatcac gcgtcgctcg atacggttac
      721 cgagaatgcg cggttttcgc ctaacgcgat ggtaatgacc tacggctaga gctagcagtg
      781 ttttctcgtt accgggacgt tcgaagccgt gcttgaagag cgatcgatta tcgccgagat
      841 cgaggggacg ttcgcatcgg gatcggccga caagcaggcg gatattcttc ggcgcgtcac
      901 cgatcttttc ctcgccggcg ccgacaacta ttccgatgac cacgtcgacc tgttcgacgg
      961 tgtgatatcg cgcatcgccg agaaaataga aaccaaggca agggccgagc tcgcccatcg
     1021 gctggcgccc gcgccaaatg cgccgccgat gaccgtgcgc aagcttgcgc aggatgagtc
     1081 gattgaggtc gcggggccga tcttgagtct gtccaacagg ctgtccgacg aagacctgct
     1141 ggcaatcgcg agcggcaaaa gtcaggaccg gcttctcgcc atttctaagc gcccctcgct
     1201 cagcgagaag gtcagcgacg tcctggtcac gcacggcaac cgcgacgtcg ttctttccgt
     1261 gacgcgtaac gaaggcgcgc ggatttcgga cgccggctac accaagctgg tcgaccggtc
     1321 gatcaacgac gaggtgctgg cgatttgcgt ctccatgcgc aaagatatcc cgcaggagca
     1381 cttcaatgca ttgatcgcca aggcttccga ggtcgtgttc gaaaagctgg ccgcgagcaa
     1441 cccgcacgcc gtctacgaag tgcacaaggt gctgaccgac atcaccgggc aggagccggc
     1501 cgggcaaccg aaagcaaagc gggactatcg cgaggcggcg gcgcagttcg acgtcgtcag
     1561 gcgatcggga actcccgtcg atccggtagt ctgcgaatac gccaagaacg gacaattcga
     1621 ggaaactgtt gccgcgctgt cagcgctgtc cggggcgccg atcaagcttg tcgaaagcgt
     1681 catgaatgac gcccggccgg atagcgattt cgcccttatt ttggccaaag ccgccggcct
     1741 ttcctggccg accgtcaagc agatatgcat tctgcgccgc aaggtctttc gcttttcgcc
     1801 gcaggcaatt gaaaacgcac gtcacagctt cgatcgcctg cagcccaaga cggcgcaacg
     1861 cctggtccgc ttctacaacg agcgtcacgc cgcgctggcg aatttccagg aactggcgca
     1921 gcacatccgc gcgcaagaaa gccccgcgcg cgccctggac gcgtagattc cgaagtttcg
     1981 aaggcggtta cgctgcgcgc cgttttgcct ggataagctt gcgattgatc aggcactctg
     2041 cgatctgcac cgcgttgagg gcggcaccct tacgcaggtt gtcggagacg acccacatct
     2101 gcaatccgtt ctcgatagtg ccgtcttcgc ggatgcggct gatataggtt gcgtcctcgc
     2161 ccacgcattc gtaaggcgtg acgtagccac cgtcctcgcg cttgtcgatc acgagacagc
     2221 cgggcgcatt ccgcaaaatc tcccgcgcct cgtccgcgct gatcggatat tcgaactcga
     2281 tgttgacggc ttccgagtgg ccgacgaaaa ccggcacgcg cacgcacgtc gcgctgagcc
     2341 tgatcttggg atcgagaatc ttctttgtct cgaccaccat cttccattct tccttcgtgt
     2401 agccgtcgtc cataaataga tcgatgtgcg ggatgacgtt gaaggcaatc cgcttcggga
     2461 acttcttggc ttctatttca tcgagcgtaa acaccgcctt gctctgcgag aacagctcgt
     2521 ccatcgcatc cttgccggcg ccggacacag actgataggt cgcaacgacg acacgcttga
     2581 tcttcgcctt gtcgtgcagc ggcttcagcg ccaccaccag ctgggcggtc gagcaattcg
     2641 gattggcgat gatgttcttc ctggtgaagc cagcgatcgc atctgcattc acttc
//
LOCUS       DASSRL010000356         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1024453, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000356 DASSRL010000000
VERSION     DASSRL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024453"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFL49_07230"
     CDS             complement(<1..337)
                     /locus_tag="VFL49_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7911805.1"
                     /translation="MKKAKKKTQAKAAASGKVIPPLAPRVSLLNRHQAVALSCAVFVF
                     AMVGAAYAAVPLYDWFCRTTGFGGTPLVAEKAPDAPIARKITVRFDGNVADGLPWTFG
                     PDKPSIEIRI"
     gene            complement(334..501)
                     /locus_tag="VFL49_07235"
     CDS             complement(334..501)
                     /locus_tag="VFL49_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911806.1"
                     /translation="MAATKHKRGIVLTPEQQRTRRVRSIALALVLAGFAILFYLVTIV
                     KLGPGVMNRPL"
     gene            complement(501..1415)
                     /locus_tag="VFL49_07240"
     CDS             complement(501..1415)
                     /locus_tag="VFL49_07240"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET7911807.1"
                     /translation="MNRAGATRSAEVLPGFSDVADYFALLKPRVMSLVVFTALVGIVR
                     AHGELHPFLGFTALLCIAIGAGASGALNMWWDADIDVRMGRTRTRPIPSGRVPAGEAL
                     AVGLTLSIASVIMLGLFAGFLAAGLLAFTIFFYAVIYTMWLKRWTAQNIVIGGAAGAF
                     PPMIGWAAATGSISLESVVLFLMIFFWTPPHFWALALLRKDDYARAGVPMLPNVAGDQ
                     ETRKQILVYAGIMAVVGVLPAILGFAGIFYGVVSTVLGALFVVFSVDVWRSDRGSKAE
                     AAAKRLFAFSVLYLFLLFAVLLVEGMGA"
     gene            complement(1417..>2356)
                     /locus_tag="VFL49_07245"
     CDS             complement(1417..>2356)
                     /locus_tag="VFL49_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7911808.1"
                     /translation="LAGAITMLLTDRNFGTTFFDPQGGGDPLLFQHLFWFFGHPEVYI
                     LVLPAFGVVSQVISTFSKKPVFGYLGMAYAMVAIGAIGFVVWAHHMYTVGMSFPAQAY
                     FMVATMVIAVPTGIKVFSWIATMWGGSIEFRTPMLWAIGFIFLFTIGGVTGVVLSNAG
                     VDRSLHDTYYVIAHFHYTMSLGATFGIFCAWYYWFPKMTGYLYSEFIGKLHFWLTFIG
                     VNLVFFPQHFLGLSGMPRRYVDYPDAFAGWNMVSSIGAAISTVGLFVFLYMIADAFLK
                     KRKAADNPWGVGATTLEWTVSSPPPFHTFETLPRIK"
BASE COUNT          549 a          724 c          735 g          348 t
ORIGIN      
        1 cgatgcggat ttcaattgac ggcttgtcag ggccgaaggt ccagggcaag ccatccgcga
       61 cgttgccgtc gaaacgcacg gtgatcttgc gcgcaatcgg cgcatcggga gctttctcgg
      121 cgacgagcgg cgtgccgcca aaaccggtag tgcggcagaa ccagtcgtac agcggaacgg
      181 cggcgtaagc ggcgccgacc atcgcgaaga cgaaaaccgc gcaagaaagc gccaccgcct
      241 gatggcggtt gagcaaactc acccgcggcg cgagcggagg aatgactttt ccgcttgccg
      301 ctgccttggc ctgcgttttc ttcttcgcct ttttcatagt ggacggttca ttactcccgg
      361 cccgagcttc acgatcgtga ccaggtaaaa caggattgca aaccccgcga gcacaagcgc
      421 gagcgcaatc gaacgcacac gccgcgtgcg ctgctgctcc ggtgtcaaca cgatcccgcg
      481 cttgtgtttt gtcgcagcca tcacgcaccc atcccttcga caagcagaac ggcgaacagc
      541 aggaagagat agagaacgga aaacgcgaac aggcgcttcg ccgccgcctc ggctttgctg
      601 ccgcggtcgc tgcgccaaac atcgaccgag aagacgacga acagcgcgcc aagaacggtg
      661 gaaacaacgc cgtagaaaat ccccgcaaaa ccgaggatcg cgggcagcac gccaaccacc
      721 gccatgatcc cggcgtagac taggatttgc ttccgcgtct cctgatcgcc cgcgacgttc
      781 ggcagcatcg gaacgcccgc gcgcgcatag tcgtccttgc gcaggagcgc gagcgcccag
      841 aaatgcggcg gcgtccagaa gaaaatcatc agaaaaagaa cgacgctttc gagactgatc
      901 gagccggtcg ccgcggccca gccgatcatc ggcgggaatg cgccggccgc accgccgatc
      961 acgatgttct gcgcggtcca gcgcttcagc cacattgtgt agatgacggc atagaagaag
     1021 atcgtgaagg ccaaaagtcc ggccgccagg aacccggcga acaggccgag catgatgacc
     1081 gacgcgatcg aaagcgtgag cccgaccgca agcgcctcgc ctgccggcac tcggcccgat
     1141 ggaatcggcc gcgtgcgggt acggcccatg cggacgtcga tatcggcgtc ccaccacatg
     1201 ttcagcgcgc cggatgcacc tgccccgatt gcgatacaga gcagcgctgt gaagccaagg
     1261 aaggggtgca gttcgccgtg cgcgcgcaca atgccgacga gcgccgtgaa cacgacgagc
     1321 gacatcacgc gcggcttcag cagcgcgaaa taatccgcaa cgtcggaaaa gcccggcagc
     1381 acctcggccg agcgtgtggc gccggcccgg ttcatctcac ttgatccgcg gcagcgtctc
     1441 gaaggtgtgg aacggcggcg gcgacgaaac ggtccactca agtgtggtgg caccaacgcc
     1501 ccacggattg tcggcagcct ttcgcttctt caagaaggcg tcggcgatca tgtagagaaa
     1561 gacgaagagg ccgaccgtcg agatcgcggc accgatagag gagaccatgt tccagcccgc
     1621 aaacgcatcc ggataatcga cgtagcgccg cggcatgccc gatagtccaa ggaaatgctg
     1681 cgggaagaag accaggttca cgccaatgaa agtgagccag aaatgcagct ttccgatgaa
     1741 ttcggaatag agatagccgg tcatcttcgg gaaccagtag taccaggcgc agaagatgcc
     1801 gaaggtagcg ccgagcgaca tcgtgtaatg gaaatgcgct atcacgtaat aggtgtcgtg
     1861 cagcgagcgg tcgacgcccg cgttcgagag cacgacgccg gtgacgccgc cgatggtgaa
     1921 gaggaagatg aacccgatcg cccacagcat cggcgtgcgg aattcgatcg agccgcccca
     1981 catcgtggcg atccacgaga acaccttgat gccggtcggc actgcgatca ccatggtcgc
     2041 gaccatgaag taagcctgcg ccgggaagct catgccgaca gtgtacatgt gatgcgccca
     2101 cacgacgaag ccgatcgcgc cgatcgcaac catggcgtaa gccatgccga gatagccgaa
     2161 cacgggtttt ttcgagaagg tggaaatcac ctggctgacc acgccgaaag cgggcagcac
     2221 gaggatgtag acctcggggt ggccgaaaaa ccagaacaga tgctggaaca gcagcggatc
     2281 gccgccgccc tgcggatcga agaaggtcgt tccgaagtta cggtcggtga gcagcatcgt
     2341 gatggcgccc gcgagc
//
LOCUS       DASSRL010000357         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_569287, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000357 DASSRL010000000
VERSION     DASSRL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569287"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="cofH"
                     /locus_tag="VFL49_07250"
     CDS             <1..1141
                     /gene="cofH"
                     /locus_tag="VFL49_07250"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HET7911809.1"
                     /translation="MIAAADELRRRTVGDVVTYVINRNINFTNVCVKACGFCAFSRGH
                     LAEEGYYLPIEEIIRRAREAHELGASEVCVQAGLAPGMDGWHYVNLCRALKNALPDLH
                     IHGFSPEEVLYGSTLTGVSIRDYLLALKDAGVGSLPGTSAEVLVDAIRQQVSPGRISV
                     DQWIELIQTAHEVGIPTTSTIMYGFIERARDKAVHLNILREIQKRTAGITEFVPLSFV
                     YEEAPMFHRKPPAGLRRGASGAEVMKMYAVSRLMLNNYIPNLQVSWVKEGPKLSQIAL
                     LAGANDFGGTLINESISTAAGASYGQLMKPSQFRGMIREMGRIPAERSTTYQKIRVFE
                     SEEQHRDLLDEINDPSQRFGSYQNLIQLKEYRFRDFYLQQKAHKH"
     gene            1190..1783
                     /locus_tag="VFL49_07255"
     CDS             1190..1783
                     /locus_tag="VFL49_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911810.1"
                     /translation="MIGDPAQFEAIGNRLGFRVVEQTPQMLRLSWQGARFPAFLCLGI
                     ALLLLFVSVPIVQALQARGFTGPAGALWYFPVMNLILFGIAIFLVTQRRVIEIDNQAR
                     QVTLRRQSLYRCQVLCAAYSEIDHVNLGVDQVYSGFALGGSTAAEKFAVPALRIGLNG
                     GAAVLLDRGSFRKLAELGKLVSERIAKPLQIDPQLQP"
     gene            complement(2075..>2560)
                     /locus_tag="VFL49_07260"
     CDS             complement(2075..>2560)
                     /locus_tag="VFL49_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911811.1"
                     /translation="AAEQKTSERDASETLAKIVYSAMAALPNWQIVSDAEVREVSQTL
                     PAGSEAERVHRLGEMVYADAVLVPRLRRFRERIGDEWGAKTPASVAFVLDLVDVRRGD
                     VIWQASFDETQKSLSENIFAIGDIGQRGVRWLSAEQLAQDGVRKAVAQLHQLIARPAA
                     S"
BASE COUNT          513 a          791 c          740 g          516 t
ORIGIN      
        1 catgattgcg gcggcggacg aattgcgccg ccgaacggtc ggcgacgtgg tgacctatgt
       61 catcaaccgc aacatcaact tcaccaacgt atgtgttaaa gcgtgcggct tttgcgcctt
      121 cagccgcggc catctggccg aggaaggcta ttacctgccg atcgaggaga ttatccgccg
      181 cgccagggaa gcgcatgagt tgggcgcgag cgaagtctgt gtccaagccg gcctcgcgcc
      241 aggcatggac gggtggcatt atgtgaacct ctgccgcgcc ctgaagaatg cgctgcccga
      301 tcttcacatc cacggctttt cgcccgaaga agttctctac ggctcgaccc tcaccggtgt
      361 gagcattcgc gactacctgc tggcgctcaa agacgccggc gtcggcagcc tgccgggaac
      421 ttcggcggaa gttctggtcg acgcaattcg ccagcaggta tcaccggggc gcatcagcgt
      481 cgaccagtgg atcgagttga tccaaaccgc ccatgaagtc ggcattccca ccacgtcgac
      541 gatcatgtac ggcttcatcg agcgcgcgcg cgacaaagca gttcatctca acatcctgcg
      601 ggaaatccaa aagcgcaccg ccggcatcac cgagttcgtg ccgctcagct tcgtttatga
      661 agaggcgccg atgtttcatc gcaaaccgcc cgccggcctg cggcgcggcg cgagcggcgc
      721 cgaagtcatg aaaatgtacg ccgtgtcgcg cctgatgctc aacaattata ttcccaatct
      781 gcaagtctcg tgggtcaaag agggtccgaa gctttcccag atcgcgctgc tcgccggcgc
      841 caacgatttc ggcggcacgc tgatcaacga gagcatctcc accgccgccg gggcatcgta
      901 cgggcagttg atgaagccgt cgcaattccg cggcatgatc cgcgaaatgg ggcgcattcc
      961 ggcggaacgc tcgaccacgt accaaaagat tcgcgtgttc gagtccgaag agcagcaccg
     1021 cgatcttctc gatgagatca atgacccgag ccagcgcttc ggttcctacc aaaatctcat
     1081 tcaattgaag gaataccgtt ttcgggattt ttatctgcaa caaaaagccc acaagcactg
     1141 agcgccgtcg cgccttcggc gcccgccccg tggctttact cattccctaa tgatcggcga
     1201 tccagcgcaa tttgaagcga tcggcaatcg cctcggcttc cgggtcgtgg agcaaacacc
     1261 gcaaatgctc cgcttgagct ggcaaggagc gcgcttccca gccttccttt gtctcggcat
     1321 cgcgctgctg ctgctgttcg tgtcggttcc catcgtgcag gcgctgcagg cgcgcggctt
     1381 caccgggccg gcaggcgcgc tgtggtattt cccggtgatg aacctgatcc tgttcggcat
     1441 cgcgatcttt ctcgtcaccc agcggcgggt gatcgagatc gacaaccagg ctcggcaagt
     1501 tacgcttcgg cgccagagtc tctatcgatg ccaggtattg tgcgcggcat acagcgaaat
     1561 cgaccatgtt aacctcggcg tcgatcaagt ctacagcggt tttgcgctgg gcggttccac
     1621 cgccgcggaa aaatttgccg tgccggcact gcgcatcggc ctgaatggcg gcgcggcggt
     1681 tttgttggat cgcggcagtt tcagaaagct cgccgagctc ggcaagctcg tcagcgagcg
     1741 catcgccaag ccgctgcaga tcgatccgca attgcaaccc tgaaataatt aacggcaggc
     1801 ggcttgcgac gatagccgta ggggcagact cccgtgtctg accttctgaa cgcctgacca
     1861 taaaggatcg gcgctgcaag ttttaatgtc tctcgggtgg ttgaaatcct cgcccttcga
     1921 gaatcctcag ggctggcttc aggcaaagtg aagtttcgat agggggcggg aacggcttgc
     1981 gccggaatga ccagcagaag aagtcgactt caagtcgacg tcggcttata ggcgaaggat
     2041 attgaattcg aaattgattc gctgaaagaa ataattacga ggccgccggg cgcgcgatca
     2101 gctgatgcaa ttgtgcgacg gcttttcgca ccccgtcttg agcaagttgc tcggcgctca
     2161 accagcgcac gccgcgttgg ccgatatcgc caatggcaaa aatattctcg ctgagcgact
     2221 tttgcgtttc atcgaagctt gcctgccaaa tcacgtcgcc gcgtctgaca tcgacgagat
     2281 ccaggacaaa cgccacggat gccggagttt tggcgcccca ttcgtcgccg atcctttccc
     2341 ggaaccggcg caaccgcggc accagcaccg cgtcggcgta aaccatttcg ccgagccggt
     2401 gcacgcgctc tgcctcgctt ccggccggca gcgtttggct gacttcacgc acctcggcat
     2461 cggaaacgat ctgccagttg ggcaaggccg ccatcgccga atagacaatc ttcgccagcg
     2521 tttcagaggc gtcgcgctcc gaggtcttct gctccgccgc
//
LOCUS       DASSRL010000358         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_849202, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000358 DASSRL010000000
VERSION     DASSRL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849202"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1219)
                     /locus_tag="VFL49_07265"
     CDS             complement(269..1219)
                     /locus_tag="VFL49_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7911812.1"
                     /translation="MRTSTKVLGLGLGLALTVAVPAAATELKMMTGPQGGSWIPLGGQ
                     LKDIWEKAIPGLAVQQSPGAGIANVRAVEEGKTDIGFSNSISAVDAIAGKAPFNKPHG
                     NVCNVATLYPQYFQFVVNADAGVNKIGDLKGKSITTQQKGNTGELITRQLLEVNGLSY
                     NDVKVSFVGYTDSVNQMKDGHAVAFGLNTQVPAGAIMDLAAARDIKLIDQSDSYEKMK
                     KINPGYKLITIPKGSYPKQDQDVKVIGFYTHIVVSCKLPADEVYTMTKTIAANTKTLA
                     TVAKDIAKLQTAEMGADIGVPFHPGAAKFYKEVGLTVPTH"
     gene            complement(1420..2400)
                     /locus_tag="VFL49_07270"
     CDS             complement(1420..2400)
                     /locus_tag="VFL49_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7911813.1"
                     /translation="MQRLLTSVFAAALAAAGLLFVQSASAFALDYPTRPPHIIVGYPA
                     GGSTDIVARLIGDWLSKRLGQQFIVENRPGAGNNLATEAVAKAAPDGYTLILVNPANT
                     INASLYKHLNFVFLRDIDPVAGVIRVPNVMEVHPSVPAKTVAEFIAYVKANPDKVNVA
                     SSGNGTSIHLSGELFKMMTGTKMTHVPYKGSAPMLTDLLGGQVQVTFDNLPSSMPHIK
                     AGKLRALAVTTAKRSEELPDVPTVAETVPGYEASAFFGFGVPHGTPKEIVDLLNKEIN
                     LALKDPTILARLKDLGGTTIPGSAADFGKHVADETAKWEKVVRAANLSIN"
     gene            2560..2934
                     /locus_tag="VFL49_07275"
     CDS             2560..2934
                     /locus_tag="VFL49_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911814.1"
                     /translation="MSSHRRWMLRDIPRTYVLLVWLAFGIAVLIYSNDWHPSGWTALR
                     HEAATAKPPAPVDNYTGSIIVVPTRGENCWQMMIDNRTGRMWDKGVVNCYEVSRPDRD
                     QRSGMSSLRINAIGKAFNRRDD"
     gene            3049..>4125
                     /locus_tag="VFL49_07280"
     CDS             3049..>4125
                     /locus_tag="VFL49_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET7911815.1"
                     /translation="MSRENDGFEFHPTRLQQFLDAAYRVINIFVRWDRLPTVMAAINI
                     AVFRDRLRAHNLHHTGYGVDSAGDWHPGYERWRSADGSYNCLAHPRMGMAGTRFARNI
                     PLSEAAPETGDALLDPSPRLISRELLARREFIPARKLNVFAAAWIQFEIHNWFSHGPP
                     ESGNEFRVSLPLGDDWPEKIDGCMLIQRTVRDKSPREPWLGAAPTFNNFNSHWWDAGQ
                     IYGNNRTKQMKIRSGHDGMIAFGADGMLPEDPAHPGVDFTGFSDNWWIGLSLLHTLFA
                     REHNTICKGLKLLYPDWDDEELFQRARLINGALIAKIHTVEWTPGILGHPALKFGMHA
                     QWSGVPYRWMQLVLGGDNEVAHGIP"
BASE COUNT          799 a         1271 c         1219 g          836 t
ORIGIN      
        1 gtgaagcagg aaaaccaaag tgagcgcgaa cagaaggtgc gtgccgcggt aatggaacgc
       61 ttcaggtgaa ccgaccgcga tcgcccagac gtggtagatc accatcgcaa cggaaatcac
      121 ggtcacagcc aggcggaaca ggccgccgta ggacagatcg accttgatgg tcatgcgctt
      181 gcccctcgat tagcgccgaa gcgatgatgg cgcggccgcc ctttcgcccg cgccgtcatc
      241 gccgtcatcg ccgtcatcgc cgtcatgatc agtgagtcgg caccgtcagg ccaacctcct
      301 tgtagaattt tgccgcaccg ggatggaacg gcacgccgat atccgcaccc atttcggccg
      361 tctgcagctt cgcgatatcc ttggcaaccg tcgcaagcgt cttggtgttc gctgcgatcg
      421 tcttcgtcat cgtgtagact tcatcggccg gcaatttgca cgacaccacg atgtgcgtat
      481 agaagccgat caccttcacg tcctgatcct gtttcggata gctgcctttc ggaatcgtga
      541 tcagtttata gccgggattg atcttcttca ttttctcgta actgtctgac tgatcaatca
      601 gcttgatgtc gcgggcggct gcgagatcca tgatggcgcc cgccggcacc tgcgtgttga
      661 gcccgaatgc cacggcatgg ccatccttca tctgattcac cgagtcggta tagccgacga
      721 agctgacctt gacgtcgttg tagctgagcc cgttcacctc cagcagctgg cgcgtaatca
      781 attcgccggt gttgcccttc tgctgcgtcg tgatgctctt gcctttgaga tcgccgatct
      841 tgttcacgcc cgcatcagcg ttgacgacaa actgaaagta ctgcggataa agcgtcgcca
      901 cgttacatac gttgccgtgc ggcttgttga acggcgcctt gccggcaatg gcatcgactg
      961 ccgagatcga attcgagaac ccgatgtcgg tcttaccttc ttcgactgca cggacgttgg
     1021 cgatgccggc gccgggcgat tgctgcacgg caaggccggg gattgctttc tcccagatgt
     1081 ctttgagctg accgcccagc ggaatccacg atccaccctg cgggccggtc atcatcttca
     1141 gttccgttgc ggcggccggt acagccaccg tcagagcaag ccccaatccg aggccgagca
     1201 ctttagtcga tgttctcatg gcgctttcct cctcacgcct gcgcttggtc gcgcgggctt
     1261 ttgctgttcg ttttgggtgg cgaatatcgt tgccccgggc agcgtacgcg gcggcgcccg
     1321 ccctgcacaa gtgctcacag gcacgcacag gcgcttggcc cgcaatgaca ggcattgcgg
     1381 gcatcaagtg ccgctgatcc cctgcggaga acttcggcgc tagttgatcg acaggttggc
     1441 cgcgcgcacg accttttccc atttggctgt ttcgtcggca acgtgcttgc cgaaatcggc
     1501 cgccgaaccg gggatggtcg tgccgccaag gtccttgagc ctggcaagga tggtcggatc
     1561 cttcagcgca agattgatct ccttgttaag aagatcgacg atctccttcg gagtcccgtg
     1621 cggcacgccg aacccgaaga acgcgctcgc ctcatagccc ggcaccgtct ccgcaacagt
     1681 cggaacgtcg ggcagctctt cggagcgctt cgccgtggtc accgccagcg cgcgcagctt
     1741 gccggctttg atgtgcggca ttgaggatgg caggttgtcg aacgtaacct gcacctgacc
     1801 gccgagcagg tcggtgagca tcggtgccga gcccttgtaa ggcacgtgcg tcatctttgt
     1861 tccggtcatc atcttgaaca gttcgccgga cagatgaatg gacgtcccgt tgcccgacga
     1921 agcgacgttc accttgtccg gattggcctt cacgtaggca atgaactcgg caacggtctt
     1981 tgccggcact gacggatgaa cttccatcac gttcggcacg cggatgacgc cggcaacggg
     2041 atcaatgtcc cgcaagaaga cgaaattgag atgcttgtag agcgaggcgt tgatggtgtt
     2101 cgccggattg accaggatca gcgtgtagcc gtctggcgcc gctttggcga cagcttcggt
     2161 cgccaggttg ttgccggcgc ctggacgatt ctcgacgatg aattgctggc cgagccgctt
     2221 gctcaaccag tcgccaatca accgcgccac gatgtcggtg gagccgcccg ccggatagcc
     2281 gacgatgatg tgaggcggcc gggtcggata gtcgagagca aatgcactgg cagactgaac
     2341 gaacaagaga cccgccgccg caagggccgc cgcaaaaacg gacgtgagta aacgttgcat
     2401 aaattgcatc cttccccatt agagcgggtg agacgttacg tggcgccgcc ggcgcagcgc
     2461 aagtcggtcc atcggctccg gaccgggccc gccgcgcgcc gggcctgaac aatctcttaa
     2521 cgatggcacc gtagatttgg caacgttatg gccgcacgca tgtcgtccca ccgccgttgg
     2581 atgttgcgcg atattccgcg cacctacgtg ctgcttgtct ggctcgcatt tggtatcgcc
     2641 gttctcatct attccaacga ctggcatccg agcgggtgga cagcgctgcg gcacgaagcc
     2701 gccacggcaa agccgccggc gcccgtcgac aattacaccg gctcgatcat tgtcgtaccg
     2761 acgcgcggcg agaactgctg gcagatgatg atcgataatc gtaccggacg aatgtgggac
     2821 aaaggcgtcg tcaattgtta cgaggtgtct cgtccggatc gcgaccaacg cagcggcatg
     2881 agttcgctcc gcatcaacgc gatcggcaag gcatttaacc gccgcgacga ctagcgcgga
     2941 cgtcatctga aaaccgagcc tatgtggccc gtttcacatt cgggcggcac gcagtatcat
     3001 taagtgcgcg gtacggtttg acaaaaacgg attgcctccg tggggcagat gtcgcgcgaa
     3061 aacgacggct tcgaatttca tccgacgcgg ctgcagcagt tcctggacgc agcctatcgc
     3121 gtcatcaaca tcttcgtgcg gtgggaccgg cttccgaccg tcatggcggc aatcaatatt
     3181 gccgtctttc gtgacagact ccgcgcccac aatcttcatc ataccggcta cggagtcgat
     3241 tccgccggag actggcatcc cggctatgag cgctggcgat ccgctgacgg gtcgtataac
     3301 tgcctcgcac acccgcgcat gggaatggcg ggtacgcggt ttgcaagaaa catcccgctg
     3361 tccgaggcgg ccccggaaac aggcgacgcc ctgctcgacc cgagcccgcg cctgatctcg
     3421 cgcgagctgc ttgcccgccg cgaatttatc ccggcccgca agctcaatgt ctttgcggcc
     3481 gcgtggatcc agttcgagat ccataattgg ttcagccacg gcccgccaga gtccggaaac
     3541 gaattcaggg tctccctgcc gcttggcgac gactggccgg agaagatcga cggctgcatg
     3601 cttatccagc gcacggttcg cgacaagtca ccacgcgaac cctggcttgg cgccgcgccg
     3661 acattcaaca acttcaattc gcattggtgg gatgccgggc agatctacgg taacaaccgc
     3721 accaagcaga tgaaaatccg ctccgggcac gacggcatga tcgctttcgg agcggacggt
     3781 atgctgccgg aagaccccgc tcatcccgga gtcgacttca ccggcttcag cgacaattgg
     3841 tggatcggac tgagcctgct gcacacgctg tttgcacgcg agcacaatac gatctgcaaa
     3901 ggactcaagc tgctctatcc tgactgggac gacgaggagc tgttccaacg tgcacgcctg
     3961 atcaacggcg cactgattgc aaaaatccac acggttgaat ggacgccggg aatcctcgga
     4021 catccggcgc tcaagttcgg catgcacgcg cagtggtctg gcgtccccta tcgttggatg
     4081 caactggtgc tgggcggaga caacgaggtc gcgcatggaa tcccc
//
LOCUS       DASSRL010000359         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_149389, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000359 DASSRL010000000
VERSION     DASSRL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149389"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFL49_07285"
     CDS             complement(<1..380)
                     /locus_tag="VFL49_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET7911816.1"
                     /translation="MNAQRLLHGIDQISYWSGKAFAWLIVALTFVVSIEVFKRYILNA
                     PTAWIFDFSNMLYGTLFMMCGAYTLALSAHVRADFVYIYLKPRGQAALDLLLYFLFFL
                     PGIIGLIYAGYGYAADSWRIGEHS"
     gene            complement(473..1654)
                     /locus_tag="VFL49_07290"
     CDS             complement(473..1654)
                     /locus_tag="VFL49_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7911817.1"
                     /translation="MSLPKNVVSQETRKVSRRNVLKAAVAAGGAAVLGFPMIAKGQTG
                     PVNMRWQSTWPTKDIFHEFALDFAKKVNDMTGGDLRIDVLPAGAVVPAFGVLDAVSKG
                     TLDGAHGVLSYHYGKSNALALWGSSPAYGMDANMVLAWHRYGGGKELLNKIYNSLGAN
                     VVSFPYGPMPTQPLGWYKKVVTKPDDFKGMKFRTVGISIDLFQGMGASVNALPGAEIV
                     PAMDRGLIEAAEFNNASSDRALGFQDVSKICMLQSYHQNAEQFEIIFNKTKFDGLPEK
                     MRAIIAYAVDAASADMSWKAIDRYSKDYVELQTKDKVRFYRTPDSVLQKQLEIFDQVE
                     AKKSAELPAFKEVAESQRAFAARAVKWDLDTNVSRRMAYNHYFAKPAAKPAAKPEAKK
                     G"
     gene            1814..>3187
                     /locus_tag="VFL49_07295"
     CDS             1814..>3187
                     /locus_tag="VFL49_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7911818.1"
                     /translation="MAAAELVRDLRAIVGAPYVLIEKEDVVVYEQDGSIFQVMPEIVV
                     LPGSSDEVAEAVKAARSAGVPIVPRGSGTGLAGGAVPAEGGIILSLARLNRILKIDLK
                     NRIAVVEPGVINADVTKAVAKDGFFYAPDPSSQAACSIGGNVANNSGGPHTLAYGVTT
                     NHVLGMEVVLDDGAIVWLGGEVPDTPGYDLCGVFVGSEGTMGIVTKVAVRLMQTRESV
                     RTLLAVFDQMDAATRTVVDITAAGIIPAALEIMDRTTIEAVERGAPVGLPRDAEAVLI
                     VEVEGLAEQTARSMEVAREICERNGAREAKVAKDETERQLLWKARKGAFGAMGALAPN
                     YYVQDGVVPRSKLPEMMRRVGEISKQFNLRIANVFHAGDGNLHPNIRFDMRAPGELER
                     VIEAGAATLRACIELGGSITGEHGVGLEKKAYIGLLFNEIDLQAMARVRRVFDPDGRF
                     NPAKLFPT"
BASE COUNT          641 a          954 c          966 g          626 t
ORIGIN      
        1 ttcgagtgct cgccgatgcg ccaggaatcc gcggcgtaac cgtagccggc atagatgagc
       61 ccgatgatgc cgggaaggaa aaataggaaa tagagcagga ggtcgagggc ggcttggccg
      121 cgcggcttta agtaaatata gacgaagtcg gcgcgcacgt gggcgctcag cgccaaggtg
      181 tacgcgccgc acatcatgaa cagcgtgccg tagagcatgt tgctgaaatc gaagatccac
      241 gccgtcggcg cattcagaat gtaccgcttg aaaacttcga tggagacgac gaacgtcagc
      301 gcgacgatca gccaggcgaa ggctttgcct gaccaatagc tgatttggtc aatgccgtgg
      361 agcagacgct gggcattcat ggcttcgaca aacccctagc tcagcgctta aaagccggcc
      421 accctgttat cggcgcatag cgggtggaac cggttggcgc atcacgaacc agttaaccct
      481 tcttggcttc cggctttgcg gcgggcttgg cagccggctt ggcaaaatag tggttgtagg
      541 ccatgcgccg gctgacgttg gtgtcgaggt cccactttac ggcccgcgcg gcaaaggcgc
      601 gctgcgattc ggcaacctct ttaaacgcag gaagctcagc cgatttcttc gcttccacct
      661 ggtcgaagat ctcgagttgc ttctgcagca ccgaatccgg tgtcctgtag aaccttacct
      721 tgtctttggt ctgcagttcc acataatctt tggagtagcg atcgatcgcc ttccacgaca
      781 tgtcggccga cgccgcgtcc acggcatagg cgatgatcgc cctcatcttt tccggaagcc
      841 cgtcaaactt ggtcttgttg aaaataatct cgaattgctc cgcgttttga tgatagctct
      901 gcagcatgca aatcttggaa acgtcctgga agcccagggc gcggtcggaa gaagcgttgt
      961 tgaactccgc cgcttcgatc aaaccgcggt ccatcgccgg cacgatctcg gcgccaggca
     1021 gggcgttcac cgaggcgccc atgccctgga acagatcgat tgatatgccc acggtgcgaa
     1081 acttcatgcc tttgaagtcg tcgggcttgg tgaccacctt cttgtaccag ccgagcggct
     1141 gggtcggcat cgggccgtag gggaaagaga ccacgttggc accgagcgag ttataaatct
     1201 tgttgagcaa ttccttgccg ccgccgtacc tgtgccaggc gaggaccatg ttggcgtcca
     1261 tgccgtaggc gggactcgat ccccagagcg ccagcgcatt gctcttgccg taatggtacg
     1321 agagtacccc atgagcgccg tcgagagtgc ccttcgacac ggcatcgagg acgccgaagg
     1381 cgggcaccac cgcccccgcg ggtaagacat cgatcctgag atcgccgccg gtcatgtcgt
     1441 tgaccttctt ggcgaagtcg agggcaaact cgtgaaaaat atccttggtc ggccaggtgc
     1501 tttgccagcg catgttgacc ggccccgttt gccctttggc gatcatcgga aagccgagca
     1561 ccgccgcgcc gccggcggcc accgccgcct tgagcacgtt gcgtcgagaa actttcctgg
     1621 tttcttggct cacaacattt tttggcaaag acataggaac ctcctttcct gatcacccta
     1681 cactgtaggg tgggatgctt atattacaat cagcggaata tcgtcaatgt cttgagtaac
     1741 cacaaaacac cagacgcttg acgtttcaag atgcgattga caccggcttc ttcgctcagt
     1801 tacgcttccc accatggcgg cagcagagtt agttcgtgac cttcgcgcca tcgtcggcgc
     1861 gccctatgtc ttgattgaaa aagaagacgt cgtcgtctac gagcaggacg gctcgatctt
     1921 ccaagtgatg ccggagatcg ttgtgcttcc cggtagcagc gacgaggtgg cggaggcggt
     1981 gaaagcggcg cgaagcgccg gcgttccaat tgtcccgcgc ggctcgggca ccggcctcgc
     2041 cggcggcgcc gtgccggctg aaggcggaat cattttgtcg ctagcgcgcc tcaaccgcat
     2101 cctgaaaatc gatcttaaaa atcgcatcgc cgtcgtcgag cccggcgtga tcaatgccga
     2161 cgtcaccaag gccgtcgcca aggacggatt tttttacgcc cccgatccga gcagccaggc
     2221 cgcctgctcc atcggcggca acgtggcgaa taattccggc gggccgcaca cgctcgctta
     2281 cggcgtcacc accaatcacg tgctcggcat ggaagtcgtc ttggatgacg gcgccatcgt
     2341 ctggctcggc ggcgaagttc cggacacgcc gggctatgat ctctgcggcg tgttcgtcgg
     2401 ctcggaaggc accatgggta tcgttactaa agtggcggtg cggctgatgc aaactcgcga
     2461 gtcggtgcgc accctgctgg cggtctttga tcaaatggac gcggcgaccc ggaccgtggt
     2521 cgatatcacc gcggcaggta tcatcccggc ggccttggag atcatggatc gcacgaccat
     2581 cgaagcggtt gagcgcggcg cgccggtcgg actgccgcgc gacgccgagg cggttttgat
     2641 cgtcgaagtc gaaggccttg ccgaacagac ggcgcgctcc atggaagtcg cgcgagaaat
     2701 ctgcgagcgc aacggcgccc gcgaagcgaa agttgccaag gacgaaaccg agcgccaact
     2761 tctgtggaag gcgcgcaaag gcgccttcgg cgccatgggc gcgttggcgc cgaattatta
     2821 tgtccaggac ggcgtcgtgc cgcgcagcaa actgcccgag atgatgcgcc gcgtcggcga
     2881 aatcagtaag cagttcaacc tgcgcatcgc caacgttttt catgccggcg acggcaacct
     2941 gcatccgaat attcggttcg acatgcgcgc gcccggcgaa ttggagcgcg tgatcgaagc
     3001 cggcgccgca accctgcgcg cctgcatcga gctcggcggc agcatcaccg gcgaacacgg
     3061 cgtcggcctg gaaaaaaagg cttacatcgg cctgctgttc aatgaaatcg atctgcaagc
     3121 catggcgcgg gtgcgccggg tgttcgaccc cgacggccgc ttcaacccgg caaaactttt
     3181 cccgacg
//
LOCUS       DASSRL010000360         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_289323, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000360 DASSRL010000000
VERSION     DASSRL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289323"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1484
                     /locus_tag="VFL49_07300"
     CDS             279..1484
                     /locus_tag="VFL49_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911819.1"
                     /translation="MRKFGWLGIAALALAVGGTAQAETVKIGVVLPFSGANADLGHQI
                     DKAFDLYVKLHAKDIAPHKVEIIKRDEGPPTGAQAKTVTTELITNDKVQLVTGFVFSP
                     SAIALAPVITQAKIPMVIANAGTAWITNLSPYIVRLSFSMWHPAYPMGAYAHDKIGCK
                     TAAMAYTDFPPGKDSTEAFKTGFEKAGGKIVDSIPMGSPVQVPDFTPFFQKIKDEHPD
                     CMYVFIPSGAHATGVMKAYGELGMRKAGIKLIGPMDLIPDNKLQDMGDAAIGTIVMGH
                     YAVDLTNPQNKSFKEEWHKAYGKDSYPDFMSAAGWDTMHAIFDTIKKLNGNMSDGAKV
                     VEALKGWSADGPRGHVMIDPQTRDIVQDEHAMEVYRKPDGKLGERILGTTKAVKDECK
                     VLKVGRCGS"
     gene            1565..2437
                     /locus_tag="VFL49_07305"
     CDS             1565..2437
                     /locus_tag="VFL49_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911820.1"
                     /translation="MPHFAIVAVSILFDGLAYAMFLFITSVGLSVTMGLMGFVNLAHG
                     AFAMAGGYVVVTLTRTLGVGFVPSLVIAAVVVGAVSVVFERGLYRRLYKSTDLEQVLL
                     TIGLAFMAIATATYIYGPIPKSVPLPDWLQGDVNLGFRNFPSYRAFLIVLGVLLIAVL
                     WYAFERTNIGAKIRAAVDNRRMAESVGINVDRLFTLTFAVGSGLAALGGGLAIQLFGL
                     SPSFAVLYLVLFLIVVAVGGLGSLKGTLLAALVLGVFDTAGKYLYSDAGGFFVYAVTV
                     AVLLVKPAGFYGRE"
     gene            2424..>3114
                     /locus_tag="VFL49_07310"
     CDS             2424..>3114
                     /locus_tag="VFL49_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7911821.1"
                     /translation="MAVSETIATAGGSRPRERAADYLLRRHRFRLAEALPWLVAIAVY
                     FVFPERMTFGSQVLVMVMFALSLDLILGYAGIVTLGHAAFFGVGAYTVGLAAARLGWS
                     EPVSGLFAAAVSAGLVGFITGWFLLRYRGLTLLMLTLATAILLQETGNLRSDISGGYD
                     GLPGLTFKPLFGVFEYDLYGHTYYLYVLAVLVVVFYFVRRIVYSPFGQTLTGIRENVR
                     RMHAIGSPVHRR"
BASE COUNT          546 a          934 c          962 g          672 t
ORIGIN      
        1 gcaatcgcgt ggaggtcgcc tcgctcgata tgaccggtga ccgaaggaag ttcgaggcga
       61 catctacaag ttcggtcgcc gcgatcaatg ccaaaagcgc agcctgatca ggcggcctta
      121 tcccgacagg ctgagcttat cgcggctgcg tcaggccgtg acgccgttgc cgattgaccg
      181 ggaccttgaa actagtccag tttaggacga gtggaccgtg gggcaggccc atgcactagt
      241 gggattccaa actattttaa gtctttaggg aggatgaaat gcgcaagttt ggttggctcg
      301 ggatagcagc cctggccctt gcggtgggcg gaacagccca ggctgaaacg gtcaaaattg
      361 gcgtggtgct gccattcagc ggagccaatg ccgatcttgg tcatcagatc gataaagcat
      421 tcgatctgta cgtgaaactt catgccaagg acatcgcgcc gcacaaagtc gagatcatca
      481 agcgtgacga aggcccgccg acgggcgcgc aggcgaagac cgtcaccacc gagcttatca
      541 ccaatgacaa agtccagttg gtgaccggct tcgtcttctc gccgtcggca atcgcgctgg
      601 cgccggtgat cacgcaggcg aagattccga tggtgatcgc caacgcgggc accgcgtgga
      661 tcaccaacct gtcgccttac atcgtgcggc tctccttcag catgtggcac ccggcttacc
      721 cgatgggcgc ctacgcgcac gacaagatcg gctgcaagac cgcggcgatg gcctataccg
      781 atttcccgcc gggcaaggac tcgaccgaag ccttcaagac cggctttgag aaagcgggcg
      841 gcaagatcgt cgattcaatt ccgatgggca gcccggtgca ggtgccggat ttcacgccct
      901 tcttccagaa gatcaaggac gagcatcccg actgcatgta cgtctttatt ccctccggcg
      961 cacacgcaac cggggtgatg aaggcttacg gcgaactcgg catgcgcaag gccggcatca
     1021 agctgatcgg cccgatggat cttattcccg acaacaagct gcaggacatg ggcgacgccg
     1081 cgatcggcac catcgtcatg ggccactatg cggtcgatct caccaatccg cagaataagt
     1141 cgttcaagga agagtggcac aaagcctacg gcaaggactc gtatccggat ttcatgtcgg
     1201 cggcgggatg ggacaccatg catgccatct tcgacaccat caaaaagctc aacggcaaca
     1261 tgtcggatgg cgccaaagtt gtcgaagcgt tgaagggctg gtcggctgat ggtcctcgtg
     1321 gccacgtgat gatcgatccg caaacacgcg atatcgttca ggacgagcac gcgatggagg
     1381 tctaccgcaa gcccgacggc aagctcggtg aacgcatcct tggaacgacc aaggcggtga
     1441 aggacgagtg caaggtgctc aaggtcggcc gctgcggctc ctgattgccg actttcgttg
     1501 acacataccg gcgccgccgc gcataaaagg cgcggcggcg tttcttcaac acgaggtggg
     1561 gctcgtgccg cattttgcga tcgttgccgt cagtatcctg ttcgacggcc tggcttacgc
     1621 catgttcctg ttcatcacct ccgtcggcct ctcggtcacg atggggctga tgggcttcgt
     1681 gaacctggcg cacggcgcgt tcgcgatggc cggcggatat gtcgtcgtca ccctcacgcg
     1741 cacgctcggc gtcggttttg ttccttcgct ggtcatcgcg gccgttgtcg tcggtgcggt
     1801 gagcgtcgtg ttcgagcgcg gtctctatcg gcggctttac aagtccaccg atctcgagca
     1861 ggtgctgctc accatcggcc ttgccttcat ggcgatcgcg accgcgactt atatctatgg
     1921 cccgattccc aaatcggtgc cgctgccgga ctggcttcag ggcgacgtta atctcggctt
     1981 tcgcaatttt ccgagctatc gcgccttcct gatcgtgctc ggtgtgctgc tcatcgccgt
     2041 gctctggtac gcattcgagc ggaccaatat tggcgccaag atccgggctg ccgtcgacaa
     2101 ccggcgcatg gctgagtcgg tcggcatcaa tgtcgatcgg ctgttcacgc tgaccttcgc
     2161 ggtcgggtcg ggacttgccg cacttggcgg cggacttgca attcagctgt ttggtctctc
     2221 cccgagcttt gccgtccttt atctcgtgct gttcctgatc gtggtggcgg tcggcggact
     2281 tggcagcctc aagggtacgc ttctggcggc gctcgtcctc ggcgtgttcg acacggccgg
     2341 aaagtatctg tactcggatg cgggcgggtt tttcgtctat gcggtgactg tggccgttct
     2401 gctggtcaag ccggcggggt tctatggccg tgagtgagac catcgccaca gcaggcggtt
     2461 cccgaccacg tgagcgcgct gcggactatc tgctcaggcg ccaccgtttc cgtctggcgg
     2521 aagccctgcc atggcttgtc gcgattgccg tctacttcgt ttttccggag cgcatgacct
     2581 tcggcagcca ggtgctggtg atggtgatgt tcgcactctc gctcgatctc attctcggct
     2641 atgccggcat tgtcacgctc ggccatgcgg catttttcgg cgtcggcgca tacacggtgg
     2701 ggctggctgc cgcacgcctg ggctggagcg agccggtcag cggcctcttt gccgcggcag
     2761 tttcggcggg cctcgtcggc ttcatcaccg gctggttcct gctgcgctat cgcggcctga
     2821 cgctgctgat gctgacgctc gcaaccgcga tcctgctgca ggaaaccggc aacctgcggt
     2881 cggacatttc cggcggctat gacggcctgc cggggctgac attcaagccg ctcttcggcg
     2941 ttttcgaata cgacctttat ggacacacat attatctgta cgtgctcgcc gtgctggtgg
     3001 tggtgttcta tttcgtgcgc aggatcgtct attcgccgtt cgggcagacg ctgaccggaa
     3061 tccgcgaaaa cgtgcggcgg atgcacgcca tcggctcgcc ggtgcatcgc cgcc
//
LOCUS       DASSRL010000361         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_61969, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000361 DASSRL010000000
VERSION     DASSRL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61969"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..912
                     /locus_tag="VFL49_07315"
     CDS             220..912
                     /locus_tag="VFL49_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7911822.1"
                     /translation="MLAPGFVYLRDVDSSIVQDIRYATHNNFTGHPLPGYTAAECVLR
                     REAAEALKQVQADLARQDLGLKVYDCYRPIRASRAMTAWAHDGNDDAATRRFYPALHK
                     RSLFALGFIAGKSRHSTGTAVDLTLVRLPAVPGRRFDPIASYGPCTGPAEQRAPDNSV
                     DMGTSFDCFDTRSYGASAAINSEQRQWRLVLQSAMRRHGFANYFREWWHYSFYGAPEP
                     RAYDFSIPPRPR"
     gene            complement(929..1435)
                     /locus_tag="VFL49_07320"
     CDS             complement(929..1435)
                     /locus_tag="VFL49_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HET7911823.1"
                     /translation="MADPQAPFWRRKAMSEMTRAEWESLCDGCGRCCLNKLIDEDTNE
                     TVFTDVGCRLLDGESCRCRDYAHRQSKVKDCVRLTARNVRRLRWLPPTCAYRLVAEGK
                     DLAWWHPLVSGDPETVHRAGVSVRGRVTASEANVSDDRLEEYIVSWPGKWPKGARRAS
                     SDRQDGTP"
     gene            1647..>2687
                     /locus_tag="VFL49_07325"
     CDS             1647..>2687
                     /locus_tag="VFL49_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7911824.1"
                     /translation="MRDLFPEHWMPNVKRFLLGLDAWIDFSLFRSASGAREAYERFST
                     FMDRFHVSGWRRWLNELLSESATLGAFGLVVLLALAIPAFRETSDDDWLKKSELAVTF
                     LDRYGNEVGSRGIRHNDSIPLDQFPDHLIKAVLSTEDRRFYEHFGIDLAGTARALVAN
                     TRAGGVVQGGSSITQQLAKNLFLSNERTLERKVKEAFLAMWLEARLTKNEILKLYLDR
                     AYMGGGAFGVDAAAQYYFNKSARDVNLAEAAMLAGLFKAPTKFAPHINLPAARARANV
                     VLDNLVEANMMTEGQVFGARRHPATPVDRRDDRAPNYYLDWAFDEMKKLVDTFPKSMT
                     ERVFVVRTALDMS"
BASE COUNT          512 a          843 c          768 g          564 t
ORIGIN      
        1 gtttttgcaa agtcatggat ctggttcgaa aacttatggc cagcgacccg ctcaatgccc
       61 tgccggtcct cgcaaagcaa gacgcagcaa tgcgggggtg ctatgtgcgg tcacgacatg
      121 aggcgagaaa cgcaccgtca gccacgggaa ctacttttgc gcttatttct tttgctgctt
      181 attgctcttc tagacgtcgc ccccgggagc gcccgcgata tgctcgcacc cggatttgtc
      241 tatctgcggg atgtcgattc gagcatcgtt caggacatcc gatacgcaac acataataat
      301 ttcacgggcc atccccttcc cggctacacc gcagctgagt gcgtactgcg gcgcgaagcg
      361 gcggaagcgc tcaaacaagt tcaggccgat ctggcgcggc aagatctcgg tctgaaagtc
      421 tacgactgtt accggccgat ccgggcttcc cgcgctatga ccgcgtgggc ccatgacggc
      481 aacgacgacg cggcaacgcg gcgtttttac ccggcacttc acaagcgcag ccttttcgcg
      541 cttggtttta tcgcggggaa atcacgacac tcaaccggca ccgcggtcga cctcaccctc
      601 gtgcggttgc cggccgtccc cggacgtcgt ttcgatccga ttgcgtccta cggtccatgc
      661 accggtcccg cggaacagcg ggctccggat aacagcgtcg acatggggac gagcttcgac
      721 tgcttcgaca ccagaagcta cggggcaagc gcggcaatca actcggagca acggcagtgg
      781 cgcctcgtgc tgcaatcggc aatgcggcgg cacggcttcg ccaactactt ccgcgaatgg
      841 tggcattact cgttttacgg tgcgccggag ccccgcgcct atgatttttc gattccgccg
      901 cggccgcgat gacgggggtt cgcttaagct agggagtgcc gtcctgccgg tctgatgacg
      961 cgcggcgggc gcctttgggc catttacccg gccaactgac aatatattcc tccagcctgt
     1021 cgtcggacac attggcttcg ctagccgtca cgcgaccgcg caccgacacg ccggcacgat
     1081 gcactgtctc aggatcgccg gataccagcg gatgccacca cgcgagatct ttgccctctg
     1141 ccaccaaccg gtaggcgcag gtcggcggta accacctgag acggcggaca ttgcgcgccg
     1201 tcagtcgaac acaatccttt accttggact ggcgatgggc gtagtcgcga cagcggcaac
     1261 tttcaccgtc gagcaaccgg caaccgacgt cggtaaacac ggtttcgtta gtgtcctcat
     1321 cgatcagctt gttcagacaa caacggccgc agccatcgca caagctttcc cattcggcgc
     1381 gagtcatctc cgacattgcc ttgcgacgcc agaagggagc ttgcgggtcg gccatgtaga
     1441 gaatctctcg atgcggtcag gttgtaactg ttcacaggcg tcccgtcacc ggaccatgcc
     1501 ggaggctcat ttcggccatt tttgacggta ttttccgttc accttgttcc agccggccgg
     1561 aacgtggcaa tggccgcagg tcggctgatc ggctaaaatt gcgtcgccgg cgcgtccggc
     1621 cggcattttt cacgagcagc acgcccttgc gcgacctttt tcccgagcac tggatgccca
     1681 acgtcaagcg gtttctgctc gggctggacg catggatcga tttcagcctc ttccgcagcg
     1741 cctccggtgc ccgcgaggcc tacgagcgct tttcgacgtt catggatcgc ttccacgtct
     1801 ctgggtggcg gcgctggctc aatgaacttc tctctgaaag tgcgacgttg ggcgcgtttg
     1861 gccttgtggt actgctggcg cttgccatcc cggcatttcg cgagacctcc gacgacgact
     1921 ggctgaaaaa atccgaattg gcagttacct ttctcgatcg ctacggcaac gaggttggct
     1981 cgcgcggaat ccggcataac gattcaatcc cgcttgatca gtttcccgac catctgatca
     2041 aggccgtgtt gtcaactgaa gaccgtcgtt tctacgagca tttcggaatc gatcttgcag
     2101 gcaccgcgcg cgctctggtc gccaacacca gagcgggagg cgttgtgcag ggcggctcat
     2161 cgatcacaca gcagctcgcc aagaaccttt tcctcagcaa tgagcggaca ctcgaacgca
     2221 aggtcaagga agctttcctt gcgatgtggc tggaagctcg cctgaccaaa aacgagatcc
     2281 tcaagctcta tctcgaccgc gcctatatgg gtggtggagc gttcggtgtg gacgccgcgg
     2341 ctcagtatta tttcaacaag tcggcacgcg atgtgaacct cgccgaggcg gcaatgcttg
     2401 ccggcctgtt caaagctccg accaagtttg cgccgcacat caacttgccg gctgctcgtg
     2461 cgcgagccaa cgtcgtactg gacaacctcg tcgaagccaa catgatgacg gaaggtcagg
     2521 tcttcggcgc ccgccgtcat cccgctacgc cagtcgaccg ccgcgacgac cgcgcgccca
     2581 attactatct cgactgggcc ttcgacgaga tgaaaaagct cgtcgataca tttccgaagt
     2641 cgatgacgga gcgggtcttc gtcgttcgca ccgcgcttga tatgagt
//
LOCUS       DASSRL010000362         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_604305, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000362 DASSRL010000000
VERSION     DASSRL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604305"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFL49_07330"
     CDS             <1..541
                     /locus_tag="VFL49_07330"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET7911825.1"
                     /translation="TFSRAGKRLRAHGLRPRRFVEETIMARPTPRQRKIMGRVMHEFE
                     HGELKSGRGGKGGKVTNRRQAIAIALHEAGASKYESESENRKTLARTERKEARGETYQ
                     QETEGKSHVGAKGRRESSPAMGGSNARHRTVKGMRAARARARDDGPTYDELYDRAKKE
                     GIEGRSKMSKQQLENALHH"
     gene            554..745
                     /locus_tag="VFL49_07335"
     CDS             554..745
                     /locus_tag="VFL49_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911826.1"
                     /translation="MKVSELMTRDVEIASPGETLEHAAKISAVLVPEPLNGGGREQDR
                     RSAISHPGGQHSQTGEKRK"
     gene            complement(789..1178)
                     /locus_tag="VFL49_07340"
     CDS             complement(789..1178)
                     /locus_tag="VFL49_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HET7911827.1"
                     /translation="MRPLLTSVAGAIAAVVSLTAIAGAQDQPAPSTRPSIEAPGAPSS
                     SSSISEEKLDAAAAAVKSVSAVKDDYEQRISRAPEGEKERIANEGTQALAKAVTDHGL
                     SVAEYTAILEVAQNDTTVRDKILQRLK"
     gene            complement(1278..1556)
                     /locus_tag="VFL49_07345"
     CDS             complement(1278..1556)
                     /locus_tag="VFL49_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911828.1"
                     /translation="MKLNSAEIEHTLDQFEAEVVPENNPVVQQLTRIFGDHTYFLDES
                     GLNIVEPAESDQQNGRMGVVVNIADWTDAGSRSLAPHEPQKTDVLVAL"
     gene            1845..3266
                     /locus_tag="VFL49_07350"
     CDS             1845..3266
                     /locus_tag="VFL49_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7911829.1"
                     /translation="MRSRVIHLINPKTDSLTTRPLYLNRALYSPLAGLLAVAACVPRD
                     RYEVVLTDENIESIDFDLKADLVGISAMTSYVNRGYEIADHFRAKGIPVVMGGVHPSF
                     MPQEALAHCDAVVIGEVELVIDRLLDDLEQGEMKGTYKSDKLHPMTGLSMPRYDLLKR
                     HRYVNKTFVQTSRGCHQGGTFCAEPLMNGLRFRYRPVDEVIHEMENCGSRTISINDAD
                     FFGTPERPKEVMRALKGRNIHWQAGVTSKLAQDDRMLELAAESGCTLLSIGFESISRA
                     TLTSVHKHVNRPENFAALVEKVHSYGIMVFGLFIYGFDGDDLSVFDDTVKFNRDAGYD
                     ACAYSTLTPYPGTLTWYEMKKANRIVSFDWTMYDQGHVVYRPAQMSGDELRLGVARAY
                     NNFYSPASIAQRFPLRGKRHRAQWVIYNLFMRRGSKTENIESIAAPTAEPGFAPMPPI
                     LPVKREWRNAVLEAAGGPGPHMG"
BASE COUNT          687 a          960 c         1022 g          669 t
ORIGIN      
        1 gaccttcagc cgtgcgggaa agcggttgcg cgcgcatggc ctgcgaccac gcagattcgt
       61 cgaggaaacg atcatggctc gtcctacacc acggcaacgc aagatcatgg gtcgcgtgat
      121 gcacgaattt gagcacggcg aacttaaaag cggccgcggc gggaagggcg gcaaggtaac
      181 aaaccgccgc caggccatcg cgatcgccct gcacgaagcc ggcgcttcca aatacgagag
      241 cgaaagcgaa aatcgaaaaa ctctggcccg taccgagaga aaggaagcgc gcggagagac
      301 gtaccagcag gaaacggaag gcaagtcgca tgtcggcgcg aaaggccgcc gtgaaagcag
      361 cccggcgatg ggcggctcga acgcccggca ccggaccgtg aaaggcatgc gtgccgcccg
      421 cgcccgcgcg cgggacgatg ggccgacata tgacgagctt tacgaccggg ccaaaaaaga
      481 agggatcgag gggcgttcaa aaatgtcgaa acagcagctt gagaacgcgc tccaccactg
      541 acagcaagca gcgatgaagg tcagtgaatt gatgacgcgc gacgtggaga tcgcaagtcc
      601 gggtgaaacc ctggagcacg ccgcaaagat cagtgctgtt ctggtgccag agcccttgaa
      661 cggaggcggc agggaacagg acaggcgctc agccatttcc catcccggcg gccagcacag
      721 ccagacaggt gaaaagcgga aataatccgt cgatcgggat gcgcccgatc gacgttttcc
      781 gatgacggtt acttcagccg ttgcaggatc ttatcacgca cggtcgtatc gttctgcgcg
      841 acttcgagaa tggcggtgta ctccgccaca gacaggccat gatcggtcac cgccttggcc
      901 agcgcttgcg tcccttcatt tgcgatacgc tccttttcac cttcgggagc ccgcgagatt
      961 cgctgctcgt aatcgtcctt gacggcggaa acgcttttca cggctgcggc cgcggcatcg
     1021 agtttctcct cggaaatgct tgacgaagaa gagggcgcgc cgggagcctc gatcgagggc
     1081 ctggtagacg gcgcaggttg gtcctgcgcg ccggcaatag ccgtcagtga gacgacagcg
     1141 gcgattgcac cggcaactga tgtcaatagt ggcctcatct tacgctcctc tttgctgatt
     1201 gcaggggtaa caacgcgccg caacgcagcg cgggcgcgct tcttctgtgt tgacggctcg
     1261 gaaacaggaa acctcgtcta gagcgcgacg aggacatctg tcttttgggg ttcgtgcggc
     1321 gcgaggctcc gcgatccggc atccgtccaa tcggcgatat tcaccacaac gcccatgcgt
     1381 ccattttgct ggtccgattc ggcaggctcg acgatgttga gcccgctctc atccagaaaa
     1441 tatgtgtggt cgccgaaaat ccgtgtgagc tgctggacga caggattgtt ctcgggaacg
     1501 acctcggctt cgaactgatc caatgtgtgc tcgatctccg cagaattaag tttcatcgcg
     1561 ttctgctctc cgtctcgggt tcaccggtgc atgtaagcgt ggacacgagc ggccgtggga
     1621 taccgtcggt tcccgcgccg gctcgtgtct cgcgcagacc taggtcaatt gatccgacga
     1681 tcaagtggtt cgcagatatt tctacgcccc gagtatttcg ctgcgcgctc ctcggtctgg
     1741 attcctgcgt cgaatcggcc aaactcttta ttcggccact ttgaggaact ttgttcacct
     1801 ttgcgtcatt ggtcgtcgtg gggcggtttt atagggaggc tcttgtgcgt tcgcgcgtca
     1861 tccatctcat caatccaaag accgattcgc tgactactcg tccgctctat ctcaaccgcg
     1921 cgctctattc gccattggca ggattgttgg cggtagcggc ctgcgttcca cgcgatcgct
     1981 atgaggtggt tctgacggac gagaacatcg aatcgatcga tttcgacctg aaggcggatc
     2041 tcgtcggcat ttctgcgatg acgagctatg tcaaccgggg gtatgaaatt gccgatcatt
     2101 tccgcgccaa aggcattccc gtcgtcatgg gcggcgtgca tccgagcttc atgccgcagg
     2161 aggcgcttgc gcactgcgat gccgtcgtca tcggtgaagt cgagctggtg attgacaggc
     2221 tgctcgatga tcttgagcag ggcgaaatga agggcaccta caagtccgac aagctgcatc
     2281 cgatgaccgg cctgtcgatg ccgcgctacg atcttctgaa gaggcaccgc tacgtcaaca
     2341 agacctttgt ccagacgtca cgcggctgcc accagggtgg cactttctgc gccgagccgc
     2401 tgatgaacgg tctgcggttc cggtatcggc cggtcgatga agtgattcat gaaatggaga
     2461 actgcggttc gcgcaccatc tcgatcaacg atgccgattt tttcggcacc ccggagcgtc
     2521 cgaaggaagt catgcgcgcg ctcaagggcc gcaatattca ttggcaggcc ggcgtgacgt
     2581 caaaactggc gcaggacgac cgcatgctcg agcttgccgc ggaaagcggc tgcacgctgc
     2641 tgagcatcgg ttttgaatcg atttcgcgcg ccacgctcac gagcgtgcac aaacatgtga
     2701 accgtcccga gaatttcgcg gcactggtcg agaaagtgca ttcctacggg atcatggtgt
     2761 tcggtctgtt catatacggc ttcgacggtg acgacctgtc ggtgttcgac gataccgtca
     2821 aattcaaccg tgacgccggt tacgacgcct gcgcctattc gacgctgacg ccttatccgg
     2881 gcacgctgac gtggtacgag atgaaaaagg caaaccgtat cgtttccttt gactggacga
     2941 tgtacgacca gggacacgtg gtctaccggc cggcacagat gtccggcgat gagcttcggc
     3001 ttggcgttgc ccgcgcgtac aataatttct attcgccggc gtcgatcgcc cagcggtttc
     3061 cactgcgcgg caagcggcat cgcgcacaat gggtgatcta caatcttttc atgcgcaggg
     3121 ggtcgaagac ggagaacatc gaatcgatcg cggcgccgac cgccgagccc ggttttgcgc
     3181 cgatgccgcc gatcctgccg gtcaagcggg aatggcggaa cgccgtgctc gaggcggcgg
     3241 gtgggccagg gccccacatg ggctgacgcg cgtcgggaca tgtgaccgga atgacagggc
     3301 tgtcataaca ggcactattg actttattcc tataggat
//
LOCUS       DASSRL010000363         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1339214, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000363 DASSRL010000000
VERSION     DASSRL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339214"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..433)
                     /locus_tag="VFL49_07355"
     CDS             complement(248..433)
                     /locus_tag="VFL49_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HET7911830.1"
                     /translation="MSMQSHLAELEKRHQALEDEINECLAHPAIDDLKIVELKRRKLQ
                     LKDQIERLRLNGNASVH"
     gene            663..869
                     /locus_tag="VFL49_07360"
     CDS             663..869
                     /locus_tag="VFL49_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HET7911831.1"
                     /translation="MTDEEERALREQLARLLQEHRDLDAAIEALVISPGSDLIQIQRL
                     KKRKLVLRDKIRRIEDQLTPDIIA"
     gene            complement(892..1515)
                     /locus_tag="VFL49_07365"
     CDS             complement(892..1515)
                     /locus_tag="VFL49_07365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7911832.1"
                     /translation="MGLPQPRLDDEFPSVTSLLAENERLKRELAVARARVGELEARAD
                     IDPLLDILNRRGFERELKRALSHVKRYGTQAALMFIDLDGFKGINDRHGHGAGDTLLK
                     AVARELIGHVRASDVVGRIGGDEFGVLVWRVEQPRAVAKARELEAVIAGVSITHGRTQ
                     IEVAASVGVAALVEDSNPADVIAAADQAMYARKDERRGLPAPVRWGE"
     gene            1651..2136
                     /gene="purE"
                     /locus_tag="VFL49_07370"
     CDS             1651..2136
                     /gene="purE"
                     /locus_tag="VFL49_07370"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7911833.1"
                     /translation="MASKPVAIIMGSQSDWATMKYAARTLDTLKIGHVARIVSAHRTP
                     KRLYEFAGSARKSGFKVIIAGAGGAAHLPGMTASLTPLPVFGVPIKTHALKGKDSLLS
                     IMQMPGGIPVGTLAIGKAGAINAALLAASVLALSDGRLAKRLDTWRARQTRAVGKRPK
                     D"
     gene            2288..>3334
                     /locus_tag="VFL49_07375"
     CDS             2288..>3334
                     /locus_tag="VFL49_07375"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7911834.1"
                     /translation="MLKPGATIGILGGGQLARMLALAAAPLGLRCHVYSPEKNSCAFD
                     VVHAGTCASYDDEGSLGRFAGAVDVITYEFENVPAKTAAFLARRRPVLPDPQVLETTQ
                     DRLIEKNFIGALKIGTAPYAPVHSATQLADGLMLAGLPAVLKTRQLGYDGKGQAKIAK
                     GGDPVAAWRSLHNAPCIVEGFVDFEREVSVVAARSRDGKVECFEVTENEHRDHILKIS
                     RVPAKVTPALANEARQIATRIAKAFDYVGVLAVEMFVVRKGSRRSLLVNEIAPRVHNS
                     GHWTIDGATVSQFEQHIRAVAGWPLAKPMRLGRRVEMTNLIGEEVDEAALWMAVPGAS
                     VHLYGKGEARAGRKM"
BASE COUNT          643 a         1067 c         1018 g          606 t
ORIGIN      
        1 ggacacgcta cacgcgctca ggatcacgac gaccgcacaa ataaagacaa cgataaatcg
       61 ggacatggcg ctctttcccc ggacctgcac tcgttttgat tgttgtcgca gactatactc
      121 gcaaatctgc cctgctgcag cgcttccgtc ccaaaatgcg gtcggaaaag acaaagccga
      181 acccaggcgt gcccgagttc ggcttgaacc ggctaggccg gaaacttcgg ccggacagtc
      241 gatccggtca gtgaacgctt gcgtttccgt tgagacgcaa tcgctcgatt tggtctttta
      301 gctgcagttt tcgccgcttg agttctacga ttttcaggtc atcgatcgct ggatgggcca
      361 ggcattcatt aatttcgtct tcgagtgcct gatgtcgttt ctccaattcc gcaaggtgcg
      421 attgcatcga catgcggtat gtctcctcga tcagtggctc tctacgatgt aagtgtaccg
      481 cagagtcgcc acaagagcga cggtacaccc gacgccggcc cgcggcaaac cggcgcaaag
      541 cctttgattg cctcgaattt ttgcgaacct tgaacgcact tgtggaaaac gcgataattg
      601 cgcgcttccg ccgacatgga gcggccaagc ggcgaccaag cgcaagcaaa tcagcggtgg
      661 cgatgaccga cgaggaagaa cgcgcgttgc gggagcagct ggcgcgcctg ctacaggaac
      721 accgcgatct cgatgcggcg atcgaagctt tggtaatttc gcccggttcc gacctgatcc
      781 agatccagcg gctcaagaag cgcaagcttg tattgcgcga caagatccgc cgtatcgagg
      841 atcagctgac tcccgacatt attgcgtaat tgcgtagccg attaccggcc tttactcgcc
      901 ccagcggacc ggggccggca atccccgccg ttcatccttg cgggcgtaca tcgcctgatc
      961 cgccgcggcg atcacatcgg ccggattcga gtcttcgaca agagccgcaa ccccgacact
     1021 cgcagcgact tcgatctgag tccggccgtg ggtgatgctc acaccggcaa tcaccgcttc
     1081 cagctcgcgc gccttcgcga ccgcccgcgg ttgctcgacc cgccagacca gaacgccgaa
     1141 ttcgtcgccg ccgatccggc cgacgacatc ggaggcgcgc acatggccga tcagctcgcg
     1201 cgcaaccgcc ttgagaagtg tatcgccggc gccgtgcccg tggcggtcgt tgatcccctt
     1261 aaagccgtcg agatcgatga acatcagggc ggcttgcgtt ccatagcgct ttacgtggga
     1321 gagagcgcgc ttgagctccc gctcgaagcc gcggcggttg aggatgtcga gcagcggatc
     1381 gatgtccgcg cgcgcttcaa gctcgccaac acgagcgcgc gcgacagcca attcgcgttt
     1441 gaggcgctcg ttctcggcga gcagcgaagt cacggatgga aattcgtcgt ccaggcgtgg
     1501 ctgcggaaga cccatggttc ggttctcgtt gtcggctctc gcgaatctta accctggacc
     1561 caacaatacc cctttcgcct tgccagtaga gggtccggac ctataatccc ccgcttcctt
     1621 ccgcatggat tggcggattc ggggagcaca atggcgtcaa aaccggtggc gatcatcatg
     1681 ggcagccagt ccgactgggc cacgatgaag tacgccgccc ggacgctcga tacgttgaaa
     1741 atcggtcacg tcgcacgaat cgtctcggcc catcgcaccc cgaaacgcct ctacgaattt
     1801 gcgggcagtg cccgcaaaag cggcttcaag gtgatcatcg ccggggccgg cggcgccgca
     1861 cacctgccgg gcatgaccgc atcactgacg ccacttccgg tgttcggcgt tcccatcaag
     1921 acgcacgcgc tcaagggcaa ggattcgctg ctctcgatca tgcagatgcc gggcggcatt
     1981 ccggtcggca cgcttgccat cggcaaagcc ggcgcaatca atgcggcgct gctggcggca
     2041 tccgtgcttg ccttgtcgga cggcaggctg gccaagcggc tcgacacctg gcgcgcgcgc
     2101 cagaccagag cggtcggcaa gcggcccaaa gactgagcat ggtgcagctg cacgggcctt
     2161 tcattccgct catccccgcg aaagcgggga tccagaattc tcggggacgg cgcgcgcggc
     2221 cccggattcc cgcttacgcg ggaataggcg gacgagatgt tgctctgact aagacaagta
     2281 gagtgacgtg ctgaaacccg gcgcgaccat cgggattctc ggcggcggac aactcgcgcg
     2341 catgctcgcg cttgcggcgg ctcctctggg tctgcgctgc cacgtttatt cgcccgaaaa
     2401 aaattcctgc gccttcgacg tcgtacacgc cggcacttgc gcgagctatg acgacgaggg
     2461 ctcgctcggc cggttcgccg gcgccgtcga tgtcatcacc tacgagttcg agaacgttcc
     2521 ggcgaagacc gccgctttcc tcgccaggcg ccggccggtg ctgcccgacc cgcaggtgct
     2581 ggagacgacg caggaccgcc tcatcgagaa gaatttcatc ggcgccctga agatcgggac
     2641 cgcaccttac gcgccggtcc acagcgcaac ccagttggcc gacgggctca tgctcgcggg
     2701 actgccggcc gtgctcaaga cccgccagct cggctacgac gggaaaggtc aggccaagat
     2761 tgcgaaaggc ggcgatccgg tcgctgcctg gcgctccctg cacaatgcgc cctgcatcgt
     2821 cgaaggcttc gtggatttcg agcgcgaggt gtccgttgtt gccgcgcgca gccgtgacgg
     2881 caaggtcgaa tgtttcgagg tgacggagaa cgagcaccgc gatcatattc tgaaaatctc
     2941 gcgcgtgccc gcgaaagtca ccccggcgct tgccaacgag gcgcgccaga tcgccacccg
     3001 cattgccaag gccttcgact acgtcggggt gctggccgtc gaaatgtttg tcgtgcgcaa
     3061 gggcagccga cgatcgcttc tggtcaacga gatcgcgcca cgcgtgcata attccggcca
     3121 ctggaccatc gacggagcaa cagtgtcgca attcgagcag cacatccgcg cggtcgcggg
     3181 atggccgctg gcaaagccca tgcgcctcgg ccgccgcgtc gaaatgacaa acctgatcgg
     3241 tgaagaagtg gacgaggccg cgctctggat ggcggtaccg ggcgcatcgg tacacttgta
     3301 cggcaagggc gaagcgcgtg cgggccgcaa gatg
//
LOCUS       DASSRL010000364         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_307298, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000364 DASSRL010000000
VERSION     DASSRL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307298"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..532
                     /locus_tag="VFL49_07380"
     CDS             368..532
                     /locus_tag="VFL49_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911835.1"
                     /translation="MRISGFRKARRKRTEPHNHSEAAVATIWLVFYVLGVVVAASSPF
                     VSRAIELASH"
     gene            543..926
                     /locus_tag="VFL49_07385"
     CDS             543..926
                     /locus_tag="VFL49_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7911836.1"
                     /translation="MKIRHVTYFLALCEEKSFTRAARRCGVAQPSLTRAIQLLEQDIG
                     GRLFERSCSNIRLTELGALVQPDFVRINQATESIESTAANFLETNADTSKQKPAHPSA
                     QTGSFRLQSAINGHSGPTQKTEDFF"
     gene            complement(1203..1988)
                     /locus_tag="VFL49_07390"
     CDS             complement(1203..1988)
                     /locus_tag="VFL49_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET7911837.1"
                     /translation="MMWCATRRFDAWLAVLVSISLVAWLLLALTWSDVTPAGFCSASL
                     RMPLSDSFNRAFVFNSPAQLASGWLLMLAAMMLPLIVAPLKHVRERSFAHRRVRAMFL
                     FVIGYGAVWMIAGVGLQVIAHAARTAVSDTLMCIALAAAVATLWQVSPAKQWCLNRCH
                     RRPQLAAFGAAANRAAFNFGLTHGASCAGACWALMLLPLFVGHGHVLTMVAVMLFALA
                     ERLESAAPLAWCWRGPGKALRIIAAQARMHLARRTSVEKVTTW"
     gene            complement(1999..>3048)
                     /locus_tag="VFL49_07395"
     CDS             complement(1999..>3048)
                     /locus_tag="VFL49_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HET7911838.1"
                     /translation="VFVPLSDVNMNALGEPDFEGVASGGSPGFGGVDTGFEHGGRTHG
                     AIETQPHDWVHGLVGGGLSPQQPGLMSDPDTAGLDPIFWLHHANIDRLWEVWRQNPIT
                     HVDPSKANWINGPAATGEHAFILPMPGGQSWTYTPGEMRDLSTLGYTYDDLSAPVPSP
                     LVARLERLGVGATEATAIVRRTAMAGSKTVELLGANTESIRLSGTEARTSIALDPAAR
                     DRLSTNLREAPATSAAPDRVFLNLENVRGISDATAFNVYINVPEGENPANYPDHLAGS
                     VALFGVRKASVADDKHAGDGLTYVLDISGIIDRLHLSRAIETGQLHVLLVPRNPVATE
                     AQISIGRISVFRQSN"
BASE COUNT          638 a          964 c          890 g          556 t
ORIGIN      
        1 gaccgagtcg ccgagatcct tgaaaaaggg atgggtgcga agcagcgtat tcctgtcgaa
       61 cacttgcgtt cgagattgcc cactcaccct tgcagccatt tcggtctaat ccaacaccaa
      121 aaccaataat cgcaaagata cgacaaaaca gcccggcagg gcagtctctt tgagtacgct
      181 gacaaccgcc aaccggcaaa tgaattccgg caatattgct ctaattgcga cccaaccccg
      241 gccggccggg cgatttatcg gtcaaacccg gcctggctcc ggccctgcaa tctttcggca
      301 aaatgtgagc tagctcaccg tgtccgtccc agcgcccgtg ctaccgatct ccaaagtgga
      361 ggtcacgatg cgcatatcgg gctttcgaaa ggcgcggcgc aagcggacag agccgcataa
      421 tcactctgaa gccgcggtgg ccaccatctg gcttgttttc tatgtgcttg gggtcgtggt
      481 cgccgcttca tcaccattcg tatcacgcgc gatcgaactc gcctcgcatt aaatccctgc
      541 caatgaaaat acgccatgtc acatattttc ttgcgctgtg cgaagagaaa agcttcacgc
      601 gtgccgcaag gcgctgcggc gtggcccaac catcgttgac gcgcgcgata cagcttctcg
      661 aacaggacat cggcggccga ctgtttgaaa gaagctgttc gaacatccgc ctgaccgaac
      721 tcggggcact ggtgcagccg gacttcgtcc gaatcaatca ggccacggaa agcatcgaat
      781 ccacggcggc caattttctg gaaacgaacg cggacacgtc caagcagaaa ccggcccacc
      841 caagcgctca gaccggctct ttccgcctgc aatcggcgat caacgggcat tcaggtccga
      901 cgcaaaaaac agaggatttc ttttgatgct taccgctgaa tggcacctga ttgacgaaag
      961 ctacggctgg cccgcattgg gtttttcggt cgcatggatg acacaaactg cgcaaaccgg
     1021 cgatgaacca tgcttgttcg ggcgtacgcc gagcgaccgt cgcgagccgt taatacgcgc
     1081 gccggcggtt catgaatgaa ttcccggatt ctcctgtttg cgcatattgg tacgagcgat
     1141 tgttaagcaa cgccgcgtct gcattacccc gaaagtggac gtctgcggtg cgctggtcga
     1201 gtctaccagg tcgttacttt ctcaacactg gtacgccgcg ccaaatgcat gcgggcttgc
     1261 gccgcaatga tccgcagggc tttgccgggt ccgcgccagc accatgcaag cggcgcagcg
     1321 ctttcgagcc gctccgcaag ggcgaaaagc atgacggcca ccatcgtcaa aacgtgtccg
     1381 tgtccgacga acagcggcag cagcatcagc gcccagcagg ccccggcgca cgaggcgccg
     1441 tgagtcagcc cgaagttaaa tgcagcgcga ttcgccgcgg caccgaacgc ggcgagttgt
     1501 ggccgacgat gacatcggtt tagacaccac tgcttggcgg gcgaaacctg ccacagcgtc
     1561 gccaccgctg ccgcaagcgc gatgcacatg agtgtatcag atacagccgt tcgcgccgcg
     1621 tgcgctatga cctgcaagcc cacgcccgca atcatccaca ctgccccata gccgatgacg
     1681 aacagaaaca tcgcacgcac tctccggtgc gcgaagctgc gttcgcgaac atgcttcagc
     1741 ggcgcgacga tcagcggcaa catcatggcg gcgagcatca gcagccagcc cgacgcgagt
     1801 tgcgccggag aattgaacac gaatgcccgg ttgaacgaat ccgacagcgg catccgcagg
     1861 ctggcggagc agaacccggc gggcgtcaca tcactccagg tgagcgccag caaaagccag
     1921 gcaaccaggc tgatcgatac gagcacggca agccatgcat cgaaccggcg cgtggcgcac
     1981 cacatcatgc gaccccgctc aattgctctg ccggaacacg cttatgcggc cgatactgat
     2041 ttgcgcttca gtcgcgacgg ggttgcgcgg caccagaagc acgtggagtt gacccgtttc
     2101 gatagcgcgg gaaagatgca gtctgtcgat gatgccggag atatcaagga cgtaggtaag
     2161 tccgtcgccg gcatgcttgt cgtcagccac cgacgccttg cgcacgccga acagcgcgac
     2221 gctgccggcc agatggtcgg gatagttggc gggattttcg ccttcgggca cgttgatata
     2281 gacgttgaag gcggtcgcgt cgctgatacc gcgcacattt tcgaggttca ggaaaacccg
     2341 gtcaggggcc gctgaggtcg cgggcgcctc gcgcaggttg gttgacaacc tgtcccgggc
     2401 cgcggggtcg agcgcgatcg aggtcctggc ctccgttccg gacaaacgga tgctctcggt
     2461 atttgcgccg agcaactcga cggtcttgga tccggccatg gcggtcctcc gaacaattgc
     2521 agtcgcctcc gtggcaccaa cgccgaggcg ttcgagccgc gccacgagcg gggacggcac
     2581 gggcgccgac agatcgtcgt aagtgtatcc gagcgtcgac agatcacgca tctcgccggg
     2641 cgtataggtc caactttgcc ctcccggcat cggcaatatg aaggcatgct cgccggtcgc
     2701 tgcgggcccg ttgatccagt tggccttgga cggatccacg tgggtgatcg ggttttgccg
     2761 ccatacctcc catagccggt caatattcgc atgatgcagc cagaagatcg gatccagccc
     2821 cgcagtgtcc ggatcggaca tcagtccagg ttgttgtggt gacagtccgc ctcccaccag
     2881 accatgcacc caatcatgcg gctgcgtctc gattgcgccg tgggtcctgc ccccatgctc
     2941 gaatccggtg tcgacgccgc cgaagcccgg gctgccgccg ctcgccactc cctcgaagtc
     3001 cggctctccg agcgcgttca tgttgacatc gctcagcggc acgaaaac
//
LOCUS       DASSRL010000365         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_657140, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000365 DASSRL010000000
VERSION     DASSRL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657140"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VFL49_07400"
     CDS             <1..1292
                     /locus_tag="VFL49_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7911839.1"
                     /translation="FILTRPVHMVFGTDEPFEGSLDTTCGDFRERTRDYWLEWVRRLA
                     FSIDWQDEIIRAGITLKLCSYEETGAIVAALTTSIPEVPKSGRNWDYRFCWMRDAFFV
                     VRALNRIGATRTMEDFISYILSVSAGRQEEVRPLYGVVHTDTLEERTAPALRGYGGDG
                     PVRIGNAAVNQNQHDVYGSIIMAATPIFFDRRLPRPGDASLFHTIEPLGEQAAKLALK
                     PDAGIWEYRGRTRVHTHSAAMCWAGCQRLAAIASNLNLPQRADYWGGIAERLGQEVLE
                     RAWNPRRKAFSAAFDSEDLDASALLLAELGLVSPDDPRFVATVEAIGLELRRGFHVMR
                     YVAADDFGLPESAFLICRFWLIDALWEIGRRDEARDMFIDALQMRNRYGLLSEDVHPV
                     TGKLWGNFPQTYSMSGLILTAIKLSRSWGDRYWRGSS"
     gene            1274..2686
                     /gene="otsA"
                     /locus_tag="VFL49_07405"
     CDS             1274..2686
                     /gene="otsA"
                     /locus_tag="VFL49_07405"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HET7911840.1"
                     /translation="MARLVVVSNRVGVPDRAARAGGLEVAIRPALKRHGGLWFGWSGR
                     VENTPRAVRSVVHDNITYATVDLSKDDYQEFYNGFANRVLWPILHYRLDLAEFTRRDL
                     GGYFRVNQYFATQLESLLQPDDVIWVHDYHLIPLAKALRDRGHNNKIGFFIHIPFPPP
                     EILAALPNHDQLIPALCHYDLVGFQTEVDAANFSRYIANECGLPWASGNSFHFGNRVV
                     QIGTFPVGVETEAFARLARRAVGTHFVREVLDSLSGRAMIIGVDRLDYSKGIPERMSA
                     FERFLANFPDWRGKVTYLQITPRSRTGIPEYADMGRAVGEAVGRINGAYGEASWTPVR
                     YINKAHSRTALAGLYRAARGGLVTPLRDGMNLVAKEFVAAQNPDDPGVLILSRFAGAA
                     HECAAALLVNPYDPEGVGNAINRALSMPLEERQQRHAATFKVLVRNDLSQWAERFLRM
                     LDRPPGTADTVPLARAAGRR"
     gene            complement(2700..>3481)
                     /locus_tag="VFL49_07410"
     CDS             complement(2700..>3481)
                     /locus_tag="VFL49_07410"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7911841.1"
                     /translation="QMADECLKINDLILSTYADFGFTGDLTVKLSTRPEKRVGSDAAW
                     DHAEDVMRDVLKRIEAQGHNQRIKTAINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQV
                     DFNLPERFEAFYIGADGAKKTPVMIHRAICGSMERFLGVLLEHYAGHLPLWLSPRQAV
                     IATITSDADSYAREAVAAARRLGLRADSDLRNEKINYKVREHSLAKVPVLLVVGKKEA
                     ADRTVSIRRLGKESQDVMPLETALKSLAEEAIPPDLRRVNK"
BASE COUNT          605 a         1065 c         1106 g          705 t
ORIGIN      
        1 cgtttattct gacgcggccg gtgcacatgg tcttcggtac ggacgagccg ttcgaaggct
       61 cgctcgatac gacctgcggg gatttccgcg agcgcacgcg tgactactgg ctggagtggg
      121 taaggcggct tgccttttcg atcgactggc aggacgagat catccgtgcc ggcatcacgc
      181 tcaagctgtg cagttatgag gagaccggtg cgatcgtcgc cgcgctcacc acctcgattc
      241 cggaggtgcc gaagtcggga cgcaactggg actatcgttt ttgctggatg cgtgacgcgt
      301 ttttcgtcgt gcgcgcgttg aaccgcatcg gggcgacgcg cacgatggaa gacttcattt
      361 cgtacatcct cagcgtttcg gccgggcggc aggaagaggt gcgcccactc tacggtgtcg
      421 tgcacactga cacgctggag gagcggacag cgccggcgct tcgcggctac ggcggcgatg
      481 ggccggtgcg catcggcaac gcggccgtca accaaaacca gcatgatgtc tacggcagca
      541 tcatcatggc tgcgaccccg atattcttcg atcgcaggct gcctcggccg ggtgatgcat
      601 cgctttttca tacgatcgag ccgcttggcg agcaggcggc gaagctcgcg ctcaagcccg
      661 atgccggcat ctgggaatat cgcgggcgca cgcgcgtgca cacgcattcg gctgcgatgt
      721 gctgggcggg ctgtcagcgg ctcgcagcga ttgcgagcaa tctcaatctg ccgcaacgcg
      781 ccgattattg gggcggcatt gccgaacgcc tcggacagga ggtgctcgag cgtgcgtgga
      841 atccccgtcg caaggccttt tcggcggcat ttgacagcga ggatctggat gccagcgcgc
      901 ttctgcttgc cgagcttggc ctggtatcgc cggacgaccc gcggtttgtg gcgaccgttg
      961 aggccatagg acttgaactt cggcgcggct ttcacgttat gaggtatgtc gctgctgatg
     1021 attttggctt gccggaatcg gcttttttga tttgccgctt ctggcttatc gatgcattgt
     1081 gggaaatcgg gcggagggat gaggcgcgcg atatgtttat cgacgcgctg cagatgcgaa
     1141 atcgatacgg cctcttgtcg gaggacgtac acccggtgac cggaaaattg tggggaaatt
     1201 ttccgcagac ctattcgatg tcaggattga ttttgacggc aatcaagctg tcgcgaagct
     1261 ggggagaccg gtattggcgc ggctcgtcgt agtctcaaac agggtcggcg tgcctgatcg
     1321 tgcggcgcgg gccggcgggc tcgaggtggc aattcggccg gcgctcaagc ggcatggcgg
     1381 cttgtggttt ggctggagcg gccgcgtcga gaacacgccg cgcgcggtca ggtccgtcgt
     1441 gcacgacaat atcacctacg ccaccgtcga cctgagcaag gatgactacc aggaattcta
     1501 caacggtttc gccaatcgcg tgctgtggcc gattctgcat taccggctcg accttgccga
     1561 attcacgcgg cgtgatctcg gaggctattt ccgggtcaac cagtatttcg ccacccagct
     1621 tgaatcgctg ctgcagcctg acgacgtgat ctgggtccac gactatcatt tgattccgct
     1681 ggccaaggcg ctgcgcgatc gcggccacaa caacaagatc ggcttcttca tacacattcc
     1741 gtttccgccg ccggaaatcc tcgccgcatt gccaaatcac gaccagctta ttccggcgct
     1801 gtgccactac gatctggtcg gcttccagac cgaggtcgat gctgccaatt tctcgcgtta
     1861 catcgccaac gagtgcgggt tgccctgggc aagcggcaac agtttccact tcggcaatcg
     1921 cgtggtgcag atcggcacct ttccggtcgg cgtggagacg gaagccttcg cgcggctcgc
     1981 ccgccgcgcc gtcggaacgc atttcgtccg cgaggtgctg gacagcctgt cgggtcgcgc
     2041 catgatcatc ggcgtcgacc ggctcgacta cagcaagggc attcccgagc gcatgagtgc
     2101 gttcgagcgc tttcttgcca attttcccga ctggcgcggc aaggtcactt accttcagat
     2161 cacgccgcgc agccggaccg gcatccccga atatgccgac atgggacggg cggtggggga
     2221 ggccgtcggc cgcatcaacg gcgcttacgg cgaagcctcg tggacgccgg tgcgctacat
     2281 caacaaggcg cacagccgca cggcgctcgc cggcctttac cgcgccgcac gcggcggcct
     2341 ggtcaccccc ttgcgcgacg gcatgaacct ggtcgcgaag gaattcgtcg ccgcgcaaaa
     2401 tccggacgat cccggcgtgc tcatcctttc gcgcttcgcg ggcgctgcgc acgaatgcgc
     2461 tgccgcgctg ctggtcaacc cttacgatcc ggaaggagtc ggcaatgcca tcaatcgcgc
     2521 attgtcgatg ccgctggaag agcgccagca gcggcatgcc gccaccttca aggtattggt
     2581 gcgcaacgat ctcagccagt gggccgagcg gtttctgcgt atgctcgatc gtcctcccgg
     2641 cacggccgat acggttccgc tcgcgcgcgc agcaggccgg cgttgaggca aagaccggac
     2701 tatttgttga cccgccgcag gtcaggcggg atcgcttcct cggcaagtga cttgagcgcc
     2761 gtctcgagcg gcatcacgtc ctggctttcc ttgccgagac ggcggatgga caccgtgcgg
     2821 tccgccgcct ctttctttcc gacaacgagc agaacgggga ccttcgcaag cgagtgctcg
     2881 cgtactttgt agttgatctt ctcgtttcgc aggtcggaat ccgcacgcag gccaagcctg
     2941 cgcgcggctg ccaccgcctc gcgcgcgtag ctgtcggcgt cggacgtaat ggtggcaatc
     3001 accgcctgcc tgggcgacag ccacaaaggc aggtggcccg catagtgctc cagcaaaacg
     3061 cccagaaagc gctccatcga gccgcaaata gcacggtgaa tcatcacagg ggttttcttc
     3121 gcgccgtcgg caccgatgta gaaagcttcg aaccgctccg gcagattgaa gtcgacctgt
     3181 gtcgtgccgc actgccagtc ccgaccgatg gcgtcgcgca ggacatattc gaacttcggt
     3241 ccgtagaaag caccttcgcc cggattgatc gcggtcttga tccgctgatt gtggccctgg
     3301 gcctcgatgc gcttgagcac atcgcgcatg acgtcttccg cgtgatccca ggctgcgtcg
     3361 gagccgacgc gcttttccgg gcgtgtggag agcttcacgg tgaggtcgcc ggtgaagccg
     3421 aaatcggcat aagtcgacag gatcagatca ttgatcttca ggcattcgtc ggccatctgc
     3481 t
//
LOCUS       DASSRL010000366         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_114767, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000366 DASSRL010000000
VERSION     DASSRL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114767"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFL49_07415"
     CDS             <1..205
                     /locus_tag="VFL49_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911842.1"
                     /translation="GWFVTEMNDDYLSGEAGTALKREIPMGRFGNPGDLDGALLLLVS
                     DAGGYITGATIVVDGGQVIQIKG"
     gene            216..1412
                     /locus_tag="VFL49_07420"
     CDS             216..1412
                     /locus_tag="VFL49_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7911843.1"
                     /translation="MDFTLAPEIEDLRLRVRSFVEEHVLPLEADPQNFSEHENIPEDR
                     LAKVREKARKIGLWAPQSPKEFGGMGLPIVAWAAIYEEAARSIFGPLAIHCMAPDDGN
                     MNLLARAGTPSQKEKWLRPLVEGKVRSAFAMTEPHPGSGSDPGMMLTRAEKKGDRYVV
                     RGHKWFITGAEGAAHHILIARTSDDKRRGLTAFLHHKDQPGWRIVRRIPIMGPHEHGG
                     HCEIEYDGLEIPEESILLNVGDGLRVTQIRLGPARLTHCMRWLGFAKRCMEIAQEYVG
                     RREGFGVRLADRENVQVKLGEIAHQIQIGRLLTMHAAWKLDQGSRARKEVSMAKVQVA
                     DALHNAADVAIQLNGARGYSKDTILEWIYRYARSARLVDGASEVHKMVLARFMREEGR
                     DFWKWG"
     gene            complement(1409..2194)
                     /locus_tag="VFL49_07425"
     CDS             complement(1409..2194)
                     /locus_tag="VFL49_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7911844.1"
                     /translation="MAYTNRMRERLARGEVALCMATRLARTADIGMIADSCGFDAFFI
                     DMEHCTISLDAAAQICVAALPVGITPMVRIAGHQFEEATRLLDMGALGIICPNVSTRA
                     EAEAFVQACCFPPLGKRSVAGAGPLQGYRQTPLGEVNKQGNASTTLIAMLETPEGIAN
                     ADAIAAVDGVDMLLIGSNDLCTAMGIPGDLKSPKLRTAYEAAATACRTHNKHLGVGGI
                     RGDVGHIAELVKLGARFVIAGSDVQYLMRSARAEVESLRRTGG"
     gene            complement(2361..3125)
                     /locus_tag="VFL49_07430"
     CDS             complement(2361..3125)
                     /locus_tag="VFL49_07430"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7911845.1"
                     /translation="MNKLASVPKISRDGISAAEWQARVDLAAVYRLTAHYGWDDVIYN
                     HSSMRVPDEPRKFLMKKHDLLYTEVTASNLVKVSMDEDLDEKSGVNRPGFTLHGGILS
                     ARPDVNCAVHVHTNVGMAIAGLKSGLRMVSQQAIRFYKRIGYHGYEGITEDFDERERL
                     NRDLGQNRAMIMHNHGLLTVGKSAREAFVLMKYLIDAADIQLKLEATGAALIEVPSDV
                     CAKTAEQYEHHDAGRGSHDWAAYLRMLDQIDTSYRN"
     gene            3232..>3706
                     /gene="dctP"
                     /locus_tag="VFL49_07435"
     CDS             3232..>3706
                     /gene="dctP"
                     /locus_tag="VFL49_07435"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET7911846.1"
                     /translation="MSKPIKIRMGGYGPQTTGFSKSLRLMGDRLEKQFGDDVAVEYIF
                     NIMDHGHKAEDILTLVENGEITLGYQSSSYLTDRVPELGFVDLPFLFSSNAQARGAMD
                     GALGEFLVRKTEERINYRILGWFENGFRHISNRLRPIHLPADMKDMKIRVLPSEIH"
BASE COUNT          723 a         1119 c         1148 g          716 t
ORIGIN      
        1 agggtggttc gtcaccgaga tgaatgacga ttatctttcc ggcgaagccg gcactgccct
       61 caagcgagag atcccgatgg gccgtttcgg caatcccggc gatctcgacg gcgcactgct
      121 gctgctggtg tcggatgcag gcggctacat caccggtgcg acgatcgttg tggacggcgg
      181 ccaggtcatc cagatcaagg gttaggaggc gacgcatgga cttcacgctt gcgcccgaga
      241 tcgaggatct tcggctgcgc gttcgttcgt tcgtggagga gcacgtgctg ccgctggaag
      301 ccgacccgca gaatttctcc gagcacgaga acattcccga ggaccggctt gccaaggtgc
      361 gcgagaaagc caggaaaatt ggtctgtggg caccgcagtc gccgaaggaa ttcggcggaa
      421 tggggctgcc gatcgtcgcc tgggcggcga tttacgaaga ggccgcacgg tcgattttcg
      481 ggccgctcgc aatccactgc atggctcccg acgacggcaa catgaacctg ctggcgcgcg
      541 cgggcacgcc gtcgcagaag gagaaatggc tgcggccgct tgtcgaaggc aaggttcgtt
      601 cggcctttgc catgaccgaa ccgcatcccg gcagcggttc tgatcccggg atgatgctca
      661 cgcgcgccga gaagaagggt gaccgttatg tcgtgcgcgg ccacaaatgg ttcatcactg
      721 gcgccgaagg cgctgcgcat cacatcctga ttgcgcgcac ctctgacgac aaacgacgcg
      781 gactcaccgc gttcctgcat cataaagacc agccaggctg gcggatcgtg cgacgtatac
      841 cgatcatggg tccgcatgag cacggcggcc attgcgagat cgagtatgac gggctggaaa
      901 ttcctgaaga gagcatcctg ctcaatgtcg gcgacggctt gcgcgtgacg cagatcaggc
      961 ttggcccggc gcggctgacg cactgcatgc gctggctcgg cttcgccaaa cgctgcatgg
     1021 aaatcgcgca ggagtatgtg gggcggcgcg aaggattcgg cgtcaggctt gccgaccgtg
     1081 agaacgtaca ggtcaagctt ggcgagatcg cacaccagat ccagatcggt cgccttctca
     1141 ccatgcatgc cgcctggaag ctcgaccagg gatcgcgcgc ccgcaaggag gtctcgatgg
     1201 ccaaggtgca ggtcgccgac gccttgcaca atgccgccga cgtcgcgatc cagctcaacg
     1261 gtgcccgcgg ttattcgaag gatacgatcc tggagtggat ctaccgctac gcccgctccg
     1321 cccgcctggt tgatggtgcg tccgaggtgc acaagatggt actggcgcgc ttcatgcgtg
     1381 aggaaggccg cgacttctgg aagtggggct agccgccggt cctgcgcaga ctttccactt
     1441 cggcgcgtgc tgatctcatc agatattgta cgtccgatcc cgcgatcacg aagcgcgcgc
     1501 cgagcttcac cagttcggca atgtgtccta cgtcgccgcg gatgccgccg acaccgagat
     1561 gcttgttgtg agtcctgcaa gctgttgcgg cggcttcata ggcggtccgc aacttcgggc
     1621 ttttgaggtc gccgggaatg cccatcgcag tgcacagatc gttcgacccg attaacaaca
     1681 tgtcgacgcc gtccaccgca gcgatcgcat cggcgttagc gatcccctcc ggcgtctcca
     1741 gcatggcaat aagcgtcgtt gaagcgttgc cctgtttgtt gacctcgccg agcggcgtct
     1801 gacgatagcc ctgcagcggc cccgcaccgg cgaccgagcg tttgcccagc gggggaaaac
     1861 agcaggcctg cacgaaggcc tcggcttccg cgcgtgtgct cacattcggg cagatgatgc
     1921 cgagcgcgcc catatcgagc aggcgtgtag cctcctcgaa ctgatggccg gcgatgcgca
     1981 ccatcggcgt tatcccgacc ggaagcgctg cgacgcagat ttgcgcggcg gcatcgagtg
     2041 aaatcgtgca gtgctccatg tcgatgaaaa aggcatcgaa cccgcaggaa tccgcgatca
     2101 ttccgatgtc ggcggtgcgg gcgaggcgcg tcgccatgca cagcgcgacc tcgccgcgcg
     2161 caagccgctc ccgcatgcgg ttcgtgtacg ccattaagga ctatccttgt catgcccggc
     2221 catagccgtt cgaagaacgg cgtgctttcg cacgcctatg cgccgggcat ccacgtcttt
     2281 cttttgttca tccgaacaag gcgtggatgg cctggacaag cccggccaag cccggccatg
     2341 acggtgaggc aggcacagca ctaattacgg taactcgtgt cgatctggtc gagcatgcgc
     2401 aggtaagcag cccagtcatg gctgccgcga ccggcgtcat gatgctcgta ttgctcggca
     2461 gtcttggcgc agacgtccga cggtacctct atcagcgcag cgccggtcgc ctcgagcttc
     2521 aattggatgt cggcggcgtc gatcaggtat ttcatcagca caaaagcttc gcgggcgctc
     2581 ttgccgacgg tgagcagccc gtgattgtgc atgatcattg cgcggttctg ccctaggtcg
     2641 cggttgagcc gctcgcgctc gtcgaaatcc tcggtaatcc cctcgtagcc gtggtagccg
     2701 atccttttgt agaagcggat cgcctgctgc gagaccatgc gcaggccgct tttgagtccc
     2761 gcaatcgcca tgccgacatt ggtgtgaaca tgcactgcgc agttcacgtc cggccgcgcg
     2821 ctgaggatgc cgccatgcag cgtgaagccg ggcctgttga cgcctgattt ttcgtcgagg
     2881 tcttcatcca tcgagacctt cacgagattg gaggcggtaa cttcggtgta aagcaagtca
     2941 tgtttcttca tgagaaactt gcgcggctca tccggaacgc gcatggacga gtggttgtag
     3001 atcacgtcgt cccagccgta atgcgcggtc aggcggtaga ccgcagcgag gtcgacgcgc
     3061 gcctgccatt cggccgcaga aattccatcc cgtgaaattt tgggaacgct ggcaagcttg
     3121 ttcatcggtg ccgtcctccg tgattgcaga acaaagtcta tcacaccggt ccgccttggc
     3181 cagattacgc gcgccggtat aagtaagaag gatcgcagcg gacaggtgaa aatgagcaag
     3241 ccaatcaaaa tccgaatggg cggctatggc ccccagacca ccgggttcag caagtcgctg
     3301 agactcatgg gcgacaggct ggaaaagcaa ttcggcgatg atgtcgctgt cgaatacatt
     3361 ttcaatatca tggaccacgg ccataaggcg gaggacattc tcacgttggt tgaaaacggc
     3421 gagatcacgc tcggctacca gtcgtcaagc tatctcaccg atcgcgttcc cgagctcggg
     3481 tttgtcgacc tgccgttcct tttttccagc aacgcgcagg cgcgcggagc aatggacggc
     3541 gcgctcggcg aattcctcgt gcgcaaaacg gaagagcgca tcaactaccg catcctcggc
     3601 tggttcgaga acggctttcg ccacatctca aacaggctac ggcccattca tctgcccgcc
     3661 gacatgaaag acatgaaaat ccgcgtgctg ccgagcgaaa ttcacc
//
LOCUS       DASSRL010000367         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_919679, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000367 DASSRL010000000
VERSION     DASSRL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919679"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="VFL49_07440"
     CDS             complement(<1..1654)
                     /locus_tag="VFL49_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET7911847.1"
                     /translation="MSNDPAENSKSKRNRRSWVTRILLVAAAVLSVTAAIPASAQTPQ
                     QKPNILFIMGDDIGWFNIGAYHQGIMSGKTPNLDKLASEGMRFTDYYAEASCTAGRAN
                     FITGELPIRTGLTTVGQAGADVGLPAQAPTIATVLKSMGYTTGQFGKNHLGDLNKYLP
                     CVHGFDEFFGYLYHLDAMSDPYWYSYPTDPAIRNAVGPRNLVHCWASDTDDPTVQPRW
                     GAIGKQKIVDEGPLAPGPNTGRTPYGGEGEGVGDAKYDMTTFDDVLVKDSIDFMKRAK
                     ESGKPFFLWHNTTRMHVFTFLAEKYKAMMNPETNYGMEEAGMTQLDDSVGALLKGLDD
                     LGIADNTIVVFSTDNGAEVFTWPDGGMTPFRATKGTVYEGGFRVPAIIRWPGKVPAGK
                     VENSIFSGLDWFPTFVAAAGNPNIVEELKAGKDLGGTTYKVHLDGYDQTDLLTSKGPS
                     KRHEIWYFAESNLGGLRLDEFKYRFIDQPEGWPGPKVPINMPILENIKQDPFERLPVS
                     NLLEGSPAYFNDFYAREFWRFVYVQQKVGQLAETAIEFPPMQK"
     gene            complement(1804..2505)
                     /locus_tag="VFL49_07445"
     CDS             complement(1804..2505)
                     /locus_tag="VFL49_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET7911848.1"
                     /translation="MVKRDAAREILGRMRERWRDPLLTVLTIVLALWMFVLAPLHESA
                     ARDTNIVGFLLAFAVAGALLVLSDKAVSAVLMLFGITLAAAAAWIRLHDPSPLDIFLN
                     ASAWIIMGLVLIWVVGRAVFAPGRITYHRIMGAILLYFAIGLTFAALYAFVGLLSADA
                     FSGMAVKDSPTLSSTMIYFSFGTLTTAGSGDIAPIQPFARSLSNVESMIGQLYPATLL
                     ARLVTLEIEDEAKRG"
     gene            complement(2541..3533)
                     /locus_tag="VFL49_07450"
     CDS             complement(2541..3533)
                     /locus_tag="VFL49_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911849.1"
                     /translation="MAYLGRFTIGAPAILLGLLAAASAAFGADLGPGPGASYVEPQPV
                     NPWKFSFTPYGWFSGINGNVTARGHTVDVDESFIEIAEKSDSFAALMGYFEARKGRFG
                     FFTDAVWADLGFPGHLNYDINRSASGNPFARLPDFKATIKGNLKINGNAQLDYQEAIV
                     QSGVFYQVAKWGDSASYTALDVMGSARYWNQDVDLSLRIRGELTADVQADFRRVGLDL
                     KRKVKGARAVAVARANDLEWVDPVVGGRMRHQFASGSEFNLEGDVGGFGAGSEFSWQA
                     VATYGFDVNVFNTTMRSVVGYRALSVDYHQEGAHGRDGLDWIQHGPVLGVVFRW"
     gene            complement(3705..>3921)
                     /locus_tag="VFL49_07455"
     CDS             complement(3705..>3921)
                     /locus_tag="VFL49_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911850.1"
                     /translation="KTLPHTIPAILTIDETLDIGSDTRTPVDDADYKVPFPFTGTINK
                     VGFKLGPSELLPPDQKAAQDMKDNADK"
BASE COUNT          748 a         1169 c         1201 g          803 t
ORIGIN      
        1 ctttctgcat tggcgggaac tcgatggcgg tttccgccag ctgcccgacc ttctgctgca
       61 catagacgaa gcgccagaat tcacgtgcgt agaagtcgtt gaagtaggct ggagaccctt
      121 ccagcagatt cgaaaccggc agccgctcga acggatcctg cttgatgttc tccaggatcg
      181 gcatgttgat cggcactttc ggccccggcc agccctccgg ctggtcgatg aatcgatact
      241 tgaactcatc gagacgcagg ccgccgaggt tggattcagc gaagtaccag atctcatgcc
      301 gcttcgacgg tcccttgctg gtaaggagat ccgtctggtc gtagccgtcg agatgcactt
      361 tgtaggtcgt gccgccgagg tccttgccgg ctttcagttc ttcgacaatg ttcgggttgc
      421 ctgccgccgc gacaaaagtc gggaaccagt cgagccccga gaagatacta ttctcgacct
      481 ttccagctgg caccttgccc ggccagcgga tgatcgccgg cacgcggaaa ccgccttcat
      541 agaccgtgcc cttggtggcg cggaacgggg tcatgcctcc gtccggccag gtgaacacct
      601 cggcgccgtt gtcggtcgaa aacacgacga tggtgttgtc agcgatgccg aggtcgtcga
      661 gtcctttcaa cagcgcacca accgagtcgt cgagctgcgt catgccggct tcttccatgc
      721 cgtagttcgt ctcgggattc atcatcgcct tgtatttttc ggcaaggaaa gtgaacacgt
      781 gcatacgcgt ggtgttgtgc cagaggaaga acggcttgcc ggactccttg gccctcttca
      841 tgaagtcgat cgagtccttg accagcacgt cgtcaaaagt ggtcatgtcg tatttggcgt
      901 cgccgactcc ctcgccttct ccgccgtaag gcgtgcgtcc ggtgttcggc cctggcgcga
      961 gcgggccttc gtccacgatc ttttgcttgc cgatcgcgcc ccagcgcggc tgaaccgtgg
     1021 gatcgtcggt gtcgcttgcc cagcaatgta cgaggttgcg cgggccaacc gcgttacgga
     1081 tcgcgggatc cgtcggatac gaataccaat acggatccga catggcgtcg aggtggtaca
     1141 gatagccgaa gaattcatcg aagccgtgca cgcaaggaag gtacttgttc agatcaccga
     1201 gatgattctt gccgaactga cccgtggtgt agcccatcga tttcagcacc gtggcgatgg
     1261 tcggcgcttg cgccggcaag ccgacatcgg cgccggcctg cccgaccgtg gtcaatcctg
     1321 tgcggatcgg caactctccg gtgatgaaat tggcgcggcc ggcagtgcag ctcgcttcgg
     1381 cgtaatagtc ggtgaaccgc atgccctccg acgccagctt gtcgaggttg ggcgtcttgc
     1441 ccgacatgat gccctggtga taggcgccga tgttgaacca gccgatgtcg tcacccatga
     1501 tgaaaagaat attcggcttc tgttggggtg tttgggcgct tgcgggtatc gctgcagtca
     1561 ccgacaacac tgccgcggcc accagcaaaa ttctggtgac ccacgatcgg cgattccgtt
     1621 ttgacttgga gttttcggca gggtcgttac tcatggcgct tcctcctcct gcttctttaa
     1681 aatttgagcg atttaaggat tatcctatgg catagattga gaccccaatc gcgaatagtc
     1741 gcacgaggcc cgctcgcgtt gggaagcttg catcaaatgc ttggcgaatt ggtgacgggc
     1801 cggtcagccg cgcttggctt cgtcctcgat ttccagggtc accagcctgg ccagcaaggt
     1861 cgcgggataa agctgcccga tcatcgattc gacattggac aggctgcggg cgaagggttg
     1921 gatcggggcg atatcgcccg acccagccgt agtcagcgtg ccgaagctga aatagatcat
     1981 ggtgctcgac agcgtcggac tgtccttcac tgccattccg gagaaagcat cggcagagag
     2041 taagcccaca aaggcataga gcgccgcaaa ggtgagcccg atggcgaaat agagcaggat
     2101 cgcgcccatg atgcgatgat aggtgatgcg tccaggcgcg aacacggcgc ggcccacgac
     2161 ccagatcagc accagcccca tgatgatcca ggcgctggcg tttaaaaaga tgtcgagcgg
     2221 cgagggatcg tgcagccgaa tccacgcagc ggcggcggca agcgtgatcc caaacagcat
     2281 cagcaccgcg gacaccgcct tgtcggagag cacgagcagc gctccagcaa ccgcaaaggc
     2341 aagcaggaaa ccgacgatgt tggtgtcgcg ggctgcgctt tcatgcagcg gggcgagcac
     2401 gaacatccag agggcaagca cgatcgtcag aaccgtgagc agcggatctc gccaccgctc
     2461 tcgcatccga ccgaggattt ctctcgcggc gtcgcgcttg accatttgtc ggaccatggc
     2521 ggcgcgcaaa tcgtgaagcg ctaccagcgg aagaccacgc ccaaaactgg gccgtgctgg
     2581 atccagtcga ggccgtcgcg gccatgcgca ccctcttggt gatagtcgac ggaaagcgcg
     2641 cgatagccga cgacgctgcg catcgtggtg ttgaagacat tcacatcgaa gccgtaggtg
     2701 gcgaccgcct gccaggaaaa ctcgctgccg gcgccaaacc cgccgacgtc gccttcgagg
     2761 ttgaactccg agcctgaggc gaattggtgc cgcatgcgcc cgccgaccac cggatcgacc
     2821 cattcaagat cgttggcgcg agcgaccgcg actgctcttg cacctttcac tttgcgcttg
     2881 aggtcgagcc cgacgcgcct gaaatcggct tggacgtcgg ccgtcagttc ccctcgaatt
     2941 ctgagcgaga gatcgacgtc ctggttccag tagcgggcgc tgcccatgac gtcgagcgcg
     3001 gtatacgacg ctgagtctcc ccatttggcg acttggtaga agacgccgga ctggacgatg
     3061 gcttcctggt agtcgagctg ggcattgccg ttgatcttga gattgccttt gatcgtggct
     3121 ttgaagtcgg ggagcctggc gaacggattg cccgacgcgc ttctattgat gtcgtagttt
     3181 agatggccgg gaaaaccgag atcggcccac accgcgtcgg tgaagaatcc gaagcggccc
     3241 ttgcgcgctt cgaaataacc catcagcgcg gcgaaactgt cgctcttctc agcgatttcg
     3301 atgaagctct catcgacatc gacggtgtgt ccgcgcgcgg tgacgttgcc gttgatgccg
     3361 ctgaaccagc catagggggt gaagctgaat ttccagggat tgaccggctg gggctcgaca
     3421 tacgaggcgc cggggcccgg cccgagatcg gcgccgaacg ctgcacttgc cgcggcgagg
     3481 aggccgagca aaatcgccgg cgctccaatt gtgaaccgtc ccagatacgc caagtccacc
     3541 cccccattgt cgccgagctg acgtggccct caattctagc agccgctgtc cgttcgccct
     3601 gatgcccttc agccacaggc cacgattatt tgccggctga gccgtgcctg aacggcctcg
     3661 tcagctcgat tcggctctct gctttccgaa cgcgatttca gctcctattt gtcggcgttg
     3721 tccttcatgt cttgcgcagc tttctggtcc ggcgggagca attccgatgg tccgagcttg
     3781 aagcccacct tgttgatggt gccggtgaac ggaaacggca ccttatagtc ggcgtcgtcc
     3841 accggcgtcc gggtgtcaga cccaatgtcg agggtctcgt cgatggtcaa gatcgccggg
     3901 atagtgtggg gcagggtctt g
//
LOCUS       DASSRL010000368         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1706847, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000368 DASSRL010000000
VERSION     DASSRL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706847"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1149
                     /locus_tag="VFL49_07460"
     CDS             280..1149
                     /locus_tag="VFL49_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7911851.1"
                     /translation="MKPCAFEYLRAETVEHACALLAEHGEEARLLAGGQSLIPMMNLR
                     LARPATLIDIGRLALNDISIAAHSIRIGALTRHCKLIESLELKRKAPIFAEAARYIAH
                     PTIRNHGTAGGSVAHADPTAEIPGLLVLLDGHVEVVSQSRVRQIAARELFRGAFSTGL
                     RPDEMITALHFQVPAGTWGACFLELAEREGDFALAAVGAVLERAGNRVTAARIVLLGA
                     DSMPVRAEAVEASLQGGPMTDAIAVKAGAEVAAGHRCYSDIRATADYRKHLLAEFTRR
                     ALLSAYDRAETQA"
     gene            1146..1625
                     /locus_tag="VFL49_07465"
     CDS             1146..1625
                     /locus_tag="VFL49_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7911852.1"
                     /translation="MKDITLTVNGVSKRVTVEPRRLLADVLRHEFGCYGVHFGCSHGA
                     CGCCTVHVNGEPRLSCLAFAVQCDGMDIVTIEGMASGEALHPVQQAFIAEHGLQCGYC
                     TPGFIMALAPFIERAEEPTDEEIRQAMSGNICRCTGYHSIVASVRRAIAERKQGADA"
     gene            1618..>3467
                     /locus_tag="VFL49_07470"
     CDS             1618..>3467
                     /locus_tag="VFL49_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7911853.1"
                     /translation="MPSRSPEQPRYVGARITRLEDPRLVTGQGRYIDDLTLPGMLHVR
                     LVRSELAHAKIVSVDTTSLQTGFPEALVFTGADIGDLAIRAAADLPGVQSYPQPLIAQ
                     QRVRFVGEPVAAVLTDDAYAVEDAAERIFVDYEPLPVLADVAAAMAAGAPRLFDAWRN
                     NLFVEQTRKGGDIDAARAQARHIVRRTYRNQRQAGVPMECRGVIAMIDPTNRLLTVWS
                     STQIPHLLRTYLAEELKWPENQIHVIAPEVGGGFGVKGHVFAEEVLVAWLAIRTRRPV
                     KWIEDRREHLIASIHAREHVHTLEAYVSAEGKVLGLRADITADVGAYSVWPFTAGSDP
                     GMVAKVLPGPYDLAAYEATYRAVATNKCPLGTYRGVGRPAAVFSQERLMDEIAIEIGI
                     DPFEMRERNLIRKFPYKNVLGYTYDEGSYALSIKKVRELLAPDIARARDPDRSNIRTG
                     VGIALFLEQTAHGTPDFVRRRSPIETGYESAKVEMHPDGKVTVDIGLQCHGQGHETTF
                     AQLVADQLGIRPEDVIVRHGSTVTSPYSVGTWGSRGITLGGGAAHRAAGVIREKLIAI
                     AAQHLQAKPQAIDLVDRCARITDDASRSIPISSLARWAQRNVENLPPGMEP"
BASE COUNT          695 a         1044 c         1037 g          691 t
ORIGIN      
        1 cccagagcaa gtgcgaagaa cgcgatcaat agccgtttca tgttcaactc cctccatccg
       61 caacgtcggc tgcgcgcgac gtcctctttc actctgacgc cctcccaatg agcgcctttg
      121 cagagatccg gtttgccggt ccgatctgtt ctgcccggaa cttgtcgaca atattaccta
      181 tcttgcatac gattgccacg cttacgtatt gtaaaaattc gggcacctgc cctctggggc
      241 cagctcccgt tatctgtcgc agagcagccg gcatcaccga tgaagccttg tgcgtttgaa
      301 tacctgcggg ccgaaacggt cgagcatgcc tgcgcactct tggcggagca cggcgaggag
      361 gcacggcttt tggccggcgg tcagagcctc atccccatga tgaatctccg gctggcgcgg
      421 cccgccaccc taatcgacat cggacggttg gcgctcaatg acatttccat cgccgcgcac
      481 agtatccgca ttggcgccct gacgcgtcac tgcaagctca tcgaaagtct cgagttgaag
      541 agaaaagcgc cgatttttgc ggaagcggcg cgttacattg cacatccaac catccgtaat
      601 catggcacgg ccggcggaag cgtggctcat gccgatccga ccgctgaaat acccgggctg
      661 ctggtcttgc tggacgggca cgttgaggtg gtttcgcaaa gtcgcgtcag gcaaattgcg
      721 gcacgcgaat tgtttcgggg agcctttagc accggcctca gacccgacga aatgattact
      781 gctttgcact tccaggttcc tgcggggact tggggagcgt gttttctgga gctggcggaa
      841 cgtgagggcg actttgcatt ggcggccgtc ggcgccgtcc tcgaacgcgc aggaaatcgc
      901 gtgaccgcag cccgtattgt gcttctcggc gccgactcga tgccagtgcg cgcggaagca
      961 gttgaggcaa gcctgcaagg cggcccgatg acggacgcaa tcgccgtgaa agcgggtgcc
     1021 gaagttgcgg cggggcatcg ctgctacagc gacatccgtg ccaccgccga ctatcgcaag
     1081 cacttgctgg ccgaattcac ccggcgcgcg ctgctgagcg cctacgatcg cgcggaaacg
     1141 caagcatgaa agatatcact cttaccgtaa atggagtgag caagagagtg accgtcgagc
     1201 cacgccgact gctcgctgac gtgctgcggc acgaattcgg gtgctatggc gtgcactttg
     1261 gatgctcaca tggtgcgtgc ggctgttgca ccgtacatgt gaacggcgag ccgcgcctct
     1321 catgtctcgc tttcgccgtg cagtgcgacg gcatggacat cgtaacgatt gaaggcatgg
     1381 catcaggcga agcccttcat ccggtccagc aggccttcat tgccgagcat gggctacaat
     1441 gcggctattg cacgccagga ttcatcatgg cgcttgcgcc gtttattgag cgtgcagaag
     1501 aaccgaccga cgaggaaatc cggcaggcga tgtctggcaa tatctgtcgc tgtacaggat
     1561 accacagcat cgttgcatcg gtccggcgcg cgatcgcaga acgaaagcaa ggcgctgatg
     1621 cctagccgat caccagaaca gccccgttat gtcggtgcgc gcatcacccg gctggaagat
     1681 ccgcgtctgg tcaccggtca agggcgctat atcgacgatt tgacgctgcc gggaatgttg
     1741 cacgtgcggc tggtccgctc ggaacttgca cacgcaaaaa tcgtgagcgt cgatacaacg
     1801 agcctgcaga ctggcttccc ggaagcgctc gtgttcactg gcgccgatat cggcgatctt
     1861 gcgatccgcg ccgccgccga cttgccgggc gtgcaaagtt acccgcagcc gctgatcgct
     1921 cagcaacgtg tcaggtttgt tggcgagcct gttgccgcag tgctcacgga cgacgcttat
     1981 gcggttgaag atgctgccga acgtattttc gtcgactatg aaccactccc tgttctcgcc
     2041 gatgtggcgg cggcaatggc ggccggcgcg ccgcgtctgt tcgacgcctg gcgcaacaat
     2101 ctgttcgttg agcagacgcg gaaaggcggc gacattgatg cggcgcgcgc gcaagcccgt
     2161 catatcgttc gacgcaccta tcgcaatcag cgtcaggccg gcgttccaat ggaatgtcgc
     2221 ggtgtcatcg caatgattga cccgacgaac cgcctcctca cggtctggtc gtcgacacaa
     2281 atcccgcatt tgctgcggac ctatttggcc gaagagctga aatggcccga aaaccaaatc
     2341 cacgtcattg cgcccgaggt cggcggcggc tttggtgtca aaggacacgt atttgccgaa
     2401 gaagtattgg tggcatggct cgccatccgt acgcgcagac ccgttaaatg gatcgaagac
     2461 cggcgcgagc acctgatcgc cagcattcac gcgcgggaac acgttcacac gctcgaagcc
     2521 tatgtgtccg ccgagggcaa ggtgctgggg ttgagggccg atatcaccgc cgacgtgggc
     2581 gcctattcgg tgtggccctt caccgccgga agcgatcccg gcatggtcgc aaaggtgctt
     2641 ccaggcccct acgatcttgc tgcatacgaa gcgacgtacc gtgctgtcgc cacgaacaaa
     2701 tgtccgcttg gcacctatcg cggcgtcggt cggccggctg ccgtattttc acaagagcgg
     2761 ctgatggacg aaatcgcgat cgagatcggt atcgatcctt tcgaaatgcg cgagcgaaat
     2821 ctcattcgca aatttcctta caaaaatgtg ctcggataca cctacgatga aggctcctat
     2881 gcgctgagca ttaaaaaggt gcgcgagctt ctggcccctg acatcgcgcg cgcaagagac
     2941 ccggatcgct caaatatccg taccggcgtg ggaatagctt tgtttctgga gcagaccgcg
     3001 cacggcacgc cggattttgt tcgtcgccgc tcgccgatcg aaaccggtta cgaatccgcc
     3061 aaagttgaga tgcacccgga cggcaaagta acggtcgata taggtttgca gtgccacggc
     3121 caagggcacg agacgacctt tgctcaactc gtcgccgacc agttgggcat tcggcccgag
     3181 gacgtgatcg tacgccacgg cagtaccgtc acgtcgccat attcggttgg aacctggggc
     3241 agccggggaa tcacactcgg cggtggcgcc gcgcatcggg cggccggcgt cattcgcgaa
     3301 aagcttatcg ccattgcagc tcaacacctg caggcaaagc cgcaggcaat cgatctcgtg
     3361 gaccggtgtg cccgcatcac cgatgatgcg tcacgttcta tcccgatttc atcgcttgcg
     3421 cgttgggctc aacggaatgt cgaaaacctg ccgccgggca tggaacc
//
LOCUS       DASSRL010000369         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_271894, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000369 DASSRL010000000
VERSION     DASSRL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271894"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /gene="pepN"
                     /locus_tag="VFL49_07475"
     CDS             complement(<1..1353)
                     /gene="pepN"
                     /locus_tag="VFL49_07475"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HET7911854.1"
                     /translation="MRTDTAQPIRLQDYHPPDWLVDGVSLDVSLHPTSARVRAILALR
                     PNPACAAAPLVLDGDGLTLVSLKLDDAPVPDGNYVVTPDGLTIPQPPNSPFTLEVETI
                     VDPSANTQLSGLYRSSGTYCTQCEAEGFRRITFFPDRPDVMAVYTTRIEAGKTEVPVL
                     LANGNLIEAGDAPDGRHYAVWHDPHPKPSYLFALVGGDLAFVEDRFVTMSGRNVTLRI
                     YVEHGKEDRCSYAMDSLKRAMRWDEKVFGREYDLDIFMIVAVSDFNMGAMENKGLNVF
                     NDKYVLASPETATDADYAHIEAVIAHEYFHNWTGNRVTCRDWFQLCLKEGLTVFRDQE
                     FSSDMRSRPVKRIADVRALRGAQFVEDAGPLAHPVRPETYREINNFYTATVYQKGSEV
                     IRMMHTILGEDGFQKGMRLYVRRHDGEAATCDQFVAAMEDANGADLGQFKLWYSQAGT
                     P"
     gene            1408..2391
                     /locus_tag="VFL49_07480"
     CDS             1408..2391
                     /locus_tag="VFL49_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7911855.1"
                     /translation="MHAMVLERPGTPLILRERPLPLAGPGEVMMSVTACGVCRTDLHV
                     VDGELSHPKLPIIPGHEIVGRVKEIGLGVDAFRVGDRVGVPWLGGTCGVCDFCRSKRE
                     NLCDKPIFTGYTRDGGFATHVVADARFCFPLTSGIDDAQIAPWLCAGLIGWRAYRMAG
                     EGKTLGLYGFGAAAHILVQVAKWQGRRVFAFTRAGDESAQRFALSLGAAWAGASDQLP
                     PDPLDAAIIFAPVGALVPIALRAAKKGGRVVCAGIHMSSIPSFPYNLLWEERHVMSVA
                     NLTRTDAHEFLDIAPKANVNCEVTRYPLAGANEALSALRDGRMQGADVIVP"
     gene            2494..3711
                     /locus_tag="VFL49_07485"
     CDS             2494..3711
                     /locus_tag="VFL49_07485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7911856.1"
                     /translation="MTIDARFVALDSCRGICAVLVVIFHFNSVLPSALADFMFVRNGY
                     LFVDFFFVLSGFVLCHKYRDRISGQNEFWQFVIRRFARVWPVHAVMLIVLFVAILCVN
                     RLPHPPELALTWDENSYSVRALLPSLFLLNAVGLEGSVWNGPAWSIGAEFQVYLVFAL
                     MTVFLYRGFVTVCAGLSIVALAFVFWRAPDLMNTTWDYGVVRCMAGFFAGVVAYHSYE
                     RLGAIDALRATMLELAAALAVALFVTCAGFGPNAVGVVSLGAPLVFGAAVVIFARERG
                     LFSLALRARPFKALGRYSLSVYLVHQPLLIMFCFAAWSAGYQTKAFAGAAGGSVVRSP
                     DLILVDFILAVVLIAAGTYRFVELPSRQRLYRYADRCIEPGSRARQRNVVARPQGRLA
                     HGLGVADHAFDRR"
     gene            complement(3664..5034)
                     /locus_tag="VFL49_07490"
     CDS             complement(3664..5034)
                     /locus_tag="VFL49_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HET7911857.1"
                     /translation="MQNEKEQVALSSIFASAGLTIAKAIVGFSTGSLAILSEAGHSLI
                     DLGATVMTYIAVRISGKPADAKHHYGHGKVEAVSALAETALLFLLSGIVVWEATKRLV
                     EHEAHIVEATVWAFAVIAVSIIVDFFRARALSHTAEKTSSHALEADALHFSSDLWSSL
                     AVLVGLVGVRFGLPWADSVAALVVAVLVCIAGWRLTRRTVDTLTDVAPPGATEKITGI
                     AARVPGVVGVEQVRARAVGEQTFIDLTIAVSRTLPLDRVNAIKEAVITALREQIAGAE
                     PVITIDPVALNTETVLDRVMVIARNRALAVHHVTVHELKDRLAVALDLEVDGKLSLQA
                     AHDLADGLESAIAAELGADVEVETHIEPLQSQDASGREAPPERVKAVAMTLSELAASG
                     RHIRDVHNVRVRETGEGEIVNFHCRVDPRLSVQSVHENVDALERALKQQSPPIKRVIG
                     HAEPMR"
     gene            complement(5092..>5802)
                     /locus_tag="VFL49_07495"
     CDS             complement(5092..>5802)
                     /locus_tag="VFL49_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7911858.1"
                     /translation="ALTAASAERILAASAKAGGVLGIGHERRYEPAMEEMRRLFDDGT
                     LGRILHMDANVSHNNFRKMDASNWRRDARHAPAGAWTALGIHLGDMFVSLAGKPARVA
                     ARTASQIFPAPSEDFVSAEIDFDNGARGRITCLSTPPFYGRFTLVCDQGWVETQEGGN
                     VDKGIPSSFVHCDPDGARQARNYKHANTVRMNFEAWADALEGRAPYRFTTDQLLGNIR
                     ILDAVARSAAADGKPVAL"
BASE COUNT         1113 a         1687 c         1752 g         1250 t
ORIGIN      
        1 gggcgtcccg gcctggctgt accagagctt gaactggccg agatcggcgc cgtttgcgtc
       61 ctccatcgcg gccacgaact ggtcgcacgt cgcggcctcg ccgtcgtgcc gccgcacata
      121 aagccgcatg cccttttgga agccatcctc gccgaggatg gtgtgcatca tgcggatgac
      181 ctcggagccc ttctggtaga cggtggccgt gtagaaattg ttgatctccc gatatgtctc
      241 gggacggacc ggatgtgcga gaggcccggc atcctcaacg aactgcgccc cgcgaagtgc
      301 gcgaacatcg gcaatacgtt tgacaggccg cgatcgcatg tccgaggaaa attcctgatc
      361 gcggaacaca gtcaggcctt ccttcaggca aagctggaac cagtcgcggc aggtaacgcg
      421 attgcccgtc caattgtgaa aatattcgtg agcgatgaca gcctcgatat gcgcatagtc
      481 cgcatcggtc gcggtttcgg gactggcaag cacgtatttg tcattgaaga cgttgagacc
      541 tttgttctcc atggcgccca tgttgaagtc ggaaacggcg acaatcatga agatgtcgag
      601 atcgtattca cggccgaata ctttctcgtc ccaccgcatg gcacgcttga gcgagtccat
      661 cgcatagctg cagcgatctt ccttgccatg ctcgacataa atccgcagcg tgacgttgcg
      721 tcccgacatt gtgacgaagc gatcctcgac aaaggcaaga tcgccgccga cgagagcaaa
      781 caaatacgac ggtttaggat gcggatcgtg ccataccgca taatgccgtc cgtccggcgc
      841 gtcgcctgcc tcgatcaagt tgccattcgc gagcaggaca gggacttccg ttttcccggc
      901 ttcgatacgc gttgtgtaga cggccatgac gtcaggccga tcgggaaaaa atgtgatgcg
      961 gcggaatcct tcggcttcgc actgcgtgca ataggtgccg ctcgagcgat agagaccgga
     1021 gagttgcgtg tttgcggatg gatcgacgat tgtctcaact tcaagcgtaa acggactgtt
     1081 cggcggctgc ggaatcgtga ggccatcagg cgtcacgaca taattaccgt caggcaccgg
     1141 tgcgtcatcg agcttaagtg aaacgagggt caatccgtcg ccgtcgagca ccaggggcgc
     1201 cgcggcgcag gctgggttcg gtctgagggc caggatcgcc cttactctcg cgctggtcgg
     1261 atgcaatgaa acatcgagtg agacgccgtc aaccagccaa tcaggcggat gatagtcttg
     1321 cagtcggatc ggctgtgcag tgtcggtacg catgcactgt gatctatgat gttccgctga
     1381 tgattggaag aaagaataat gcaacacatg catgccatgg tgcttgagcg cccaggaacg
     1441 ccgctcatct tgcgcgagcg gcctttaccg ctggcgggcc ccggcgaagt catgatgtct
     1501 gtgacagcct gcggggtttg ccgtaccgac ctgcatgtgg tcgacggaga attgtcgcat
     1561 cccaaattgc cgatcatccc cggtcacgaa atcgtcggcc gcgtgaaaga aattgggctg
     1621 ggcgtggatg cattcagggt cggcgatcgc gtgggcgtgc cgtggctggg agggacctgc
     1681 ggcgtttgcg acttttgccg cagtaagcgg gaaaacctgt gcgataagcc gatctttacc
     1741 ggctatacgc gggatggcgg cttcgccacc cacgtggtcg cggacgcccg tttctgcttt
     1801 ccgctcacca gcggcatcga tgacgcacaa atcgcgccct ggctttgtgc gggcctgatc
     1861 ggctggcggg cctaccgcat ggccggcgaa ggcaaaacgc tcggcttata cggatttggt
     1921 gctgcggcgc acattctggt gcaagttgca aaatggcaag ggcggcgtgt tttcgcattc
     1981 acgcgcgcag gcgatgagtc ggcgcaacgc ttcgcgctct cactaggtgc cgcctgggcc
     2041 ggcgcctccg atcaattgcc gcctgatccg ctcgacgcag cgattatctt tgcgcctgtc
     2101 ggtgcgctcg taccgattgc acttagagcg gcaaagaaag gaggccgcgt cgtctgcgca
     2161 ggtattcata tgtcgagcat accgagcttt ccctataatc tgctctggga ggaacggcat
     2221 gtgatgtcgg ttgcgaatct cacccgcact gatgcacacg agtttctcga cattgctccc
     2281 aaggcgaatg tcaactgcga ggtaacgcgc tatccgctgg cgggagccaa cgaggcctta
     2341 tccgctctgc gcgatggccg tatgcagggc gcggatgtca ttgtgcccta gggccgccga
     2401 agcaacgaca caaactttcc atagtttttg ttggaacgaa caagcgtcgc tgtcgttgcg
     2461 tttccgaccg gtcattcggt ggggacaatc cagatgacga tagacgcgcg gttcgtagcg
     2521 ctcgattcgt gtcgaggaat ctgcgccgtc ctggtcgtca tatttcactt taattccgtt
     2581 ctgccgagcg cgcttgcaga cttcatgttt gtgcgtaatg gttatctgtt cgtcgacttc
     2641 tttttcgtat tgagtggctt tgttctctgc cacaaatacc gcgacagaat ctccggacaa
     2701 aatgagttct ggcagtttgt gattcgtcga tttgcgcgcg tgtggccggt acatgcggtg
     2761 atgctcatcg tcctcttcgt cgcgatcttg tgcgttaatc ggcttccgca cccgcccgaa
     2821 cttgcgctga cctgggatga aaacagctat tctgttcggg cccttcttcc gagccttttc
     2881 ttgctgaacg cggtcgggct ggaggggtcg gtctggaacg gcccggcctg gagcatcggc
     2941 gccgaattcc aggtttatct ggttttcgca ctgatgactg tatttctgta ccggggattc
     3001 gtgactgtct gcgccggact atcaatcgtt gcacttgctt tcgttttttg gcgcgcgcct
     3061 gatctcatga acacgacatg ggactacggt gtcgttcgct gcatggccgg cttttttgca
     3121 ggcgttgtcg cttaccactc ttacgaaaga cttggcgcca tcgacgcact gagagcgacg
     3181 atgctggaat tggcagcggc gttggccgtg gcgctgttcg tgacctgcgc cgggttcggc
     3241 ccgaacgccg tgggcgtcgt cagcctcgga gcaccattgg tattcggggc tgcagttgtc
     3301 atttttgcgc gggagcgagg acttttctcg ctggccttac gtgcgcgacc gttcaaggcg
     3361 ctggggcgat attcgctttc ggtctacctc gtacaccagc cgctgttgat catgttctgt
     3421 tttgccgcct ggtcggccgg ctatcagaca aaggcatttg caggcgccgc gggcggctca
     3481 gttgtcaggt ctcccgatct cattttggtt gattttatcc ttgcggttgt actgatcgcg
     3541 gccggtacct accgctttgt cgaattacca tcgcgccagc gactttaccg atatgcggac
     3601 aggtgtatcg aaccgggcag tcgagcgcga caacgaaacg tcgtggccag gccacagggc
     3661 aggctagcgc atgggctcgg cgtggccgat cacgcgtttg atcggcggtg attgctgctt
     3721 gagcgcacgc tcgagggcgt caacattctc atgaacgctt tggacgctca aacgcgggtc
     3781 gacacgacag tgaaaattga cgatttcgcc ttcgccggtc tcacgcaccc gcacattgtg
     3841 cacgtcgcgg atatggcggc cgctggctgc cagctcggat agcgtcattg cgacggcctt
     3901 gacccgctcc ggtggtgctt cgcgtccgga agcatcctgc gactgcagcg gttcgatatg
     3961 ggtttcgacc tcgacatcgg cgccaagttc ggcggcaatg gcgctctcaa gcccgtccgc
     4021 caaatcgtgc gcggcctgca gggagagctt gccgtcgacc tcgaggtcga gcgcgaccgc
     4081 aagcctgtcc ttaagctcgt gcacagtgac gtgatgcacg gcaagcgcgc gattgcgcgc
     4141 aatgaccata acgcgatcaa gcacggtttc ggtattaagc gcgaccggat cgatcgtaat
     4201 caccggctcg gcacctgcta tttgctcacg caacgcagtg atgacggctt ctttgatcgc
     4261 gtttacgcgg tcgagcggca gcgtacggct gaccgcaatc gtcaaatcga tgaacgtttg
     4321 ctcgccgacc gcgcgcgcac gtacctgctc tacgccgaca acgccgggga ctcgagcggc
     4381 aataccagtg atcttttcgg tcgcccccgg cggcgcaacg tcggtcaatg tgtcgacggt
     4441 gcgtcttgtg agcctccaac cggcgatgca aacgagtact gccacaacca atgcggccac
     4501 ggagtccgcc cacggtaatc cgaaccgtac gccgacgagt cctaccagca ctgccagtga
     4561 ggaccacaga tcggaggaaa aatgaagtgc gtcagcttca agcgcgtgac tcgaagtctt
     4621 ttccgctgtg tgagacaggg cgcgggcgcg gaagaagtcg acaatgatcg agaccgcgat
     4681 aacggcgaat gcccacacag tcgcttcgac tatatgagcc tcatgctcaa ccaggcgctt
     4741 ggtcgcttcc cacacgacta ttccggaaag gagaaacaac agagcggttt cggcaagggc
     4801 cgaaacggcc tccactttgc cgtgcccgta gtgatgcttt gcgtccgccg gtttgcccga
     4861 aattcgcacg gcgatgtagg tcatgaccgt ggcgccgagg tcgatcagcg aatggccggc
     4921 ttccgacaga atcgcgagcg aaccggtgga aaatccgaca atggccttgg cgatcgtcaa
     4981 ccccgccgag gcgaagatcg aggacagcgc aacttgttct ttttcgttct gcatacagcg
     5041 ccggtgtagc ccaaacgcgg gaaaaattcg aggaccagcg gcgctttggt gtcaaagcgc
     5101 aacaggcttt ccatccgccg ctgcagagcg cgccactgca tcgagaatac gaatattgcc
     5161 aagcagctga tcggtcgtaa aacgataagg cgcacgcccc tcaagcgcat cggcccaggc
     5221 ctcgaaattc atgcgtacgg tgttggcatg cttgtaattt cgggcttgcc gcgcgccatc
     5281 cggatcgcaa tgaacgaaac tcgatggaat gcccttgtcc acattgcctc cttcctgcgt
     5341 ctcgacccaa ccctggtcgc aaaccagcgt aaagcgccca taaaacggcg gcgtcgacag
     5401 gcatgtgatc cgcccgcgag cgccgttgtc gaagtcgatt tcggcgctga caaaatcctc
     5461 agacggtgcc ggaaaaatct gggacgcggt gcgggctgcg acgcgcgccg gcttgccggc
     5521 aagggacacg aacatgtcgc caagatgaat tccgagcgct gtccaggcgc cggccggagc
     5581 gtgacgggcg tcacgccgcc aattcgacgc atccattttc cggaagttgt tgtgacttac
     5641 attggcgtcc atgtgcaaaa tgcggccgag tgttccgtca tcgaacaggc ggcgcatttc
     5701 ctccatcgca ggctcgtacc gccgttcgtg gccgatcccg agcacgccgc cggcttttgc
     5761 gctggcggcc aaaatccgct ccgcgctggc cgcggtaagc gc
//
LOCUS       DASSRL010000370         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_902527, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000370 DASSRL010000000
VERSION     DASSRL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902527"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFL49_07500"
     CDS             <1..592
                     /locus_tag="VFL49_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
                     /protein_id="HET7911859.1"
                     /translation="QKPVAEQGSGEMAEAGRAYMASRTADVKAMSGHAWGNAYTRSLL
                     GVRLGLNGTGDWSMVKPFAAPPPVVVGDRAPDGVVHDSEGHETRLHDLFGRSFVALYF
                     TDVRRRPDIPANDVPWLRHYAISRWDAPRDSAVRSCALLDSGNRVTQRYGCQPDTVVL
                     VRPDDHIAAIEPMKSGLADIAYQAALRPMQRQAVPA"
     gene            589..1347
                     /locus_tag="VFL49_07505"
     CDS             589..1347
                     /locus_tag="VFL49_07505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7911860.1"
                     /translation="MTISVSYKFDEAVVLITGAGSGIGAALARHCAREGASVIAIDRE
                     HDNLLRDLDPALRKKIDVRQVDVSNESAVTGLLDDVRFKYPRLDVAVLAAAIQVRTNI
                     DDMTASEWRSVIDTNVNGIFYCLHAIIPLMKAQRSGAIVAFTSGLALIGWPGASAYAA
                     SKAALIGMIKSAAQELRNYNVRANVLSPGVRATPIFMDVSTETEREHYRNSIGIGEPE
                     GVVPTLLYLISDASANLTGAVLEHRFTPVNEQTQ"
     gene            1422..>2012
                     /locus_tag="VFL49_07510"
     CDS             1422..>2012
                     /locus_tag="VFL49_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911861.1"
                     /translation="MKKVIAAFLCSAWLVLGGVIGGSGQAQAQEKKLTDITFSLDFIT
                     LGRHAPFYVAIDKGFYKEEGLNVNIVPAKGTAQGIQNVESGIAQLGFVDVASLVVARA
                     EGSTVKVVSVIYQKAPFCFFSLDPGANVTKLKDFEGLKVGSNTGSYITNIARAMMRKA
                     GLDPNKLQMESIEPSARIAMLATRKIPAIDFFIITKP"
BASE COUNT          394 a          620 c          636 g          362 t
ORIGIN      
        1 gcagaagccg gtcgcggagc agggctcggg cgaaatggcg gaagccggac gggcctacat
       61 ggcaagccgc accgccgacg tgaaagcgat gagcgggcac gcctggggca atgcctatac
      121 gcgttcgctg ctcggcgtcc gtctcggtct caacggcacc ggcgattggt cgatggtgaa
      181 gcccttcgcg gcgccgccgc cggtcgttgt cggggaccgc gcgccggacg gtgtcgtgca
      241 tgacagcgaa ggacacgaga cccgcctgca cgaccttttc ggccgatcct ttgtcgcgct
      301 ctatttcacc gacgtgcggc ggcgcccgga tattcccgca aacgacgtcc cctggctcag
      361 acactatgcg atctcgcgct gggatgcgcc gcgcgattca gccgtgcgca gctgcgcgct
      421 gctcgattcc ggcaatcgcg tgacgcagcg ttatggctgc cagcccgaca ccgtggtgct
      481 ggtgcgtccg gatgaccaca tcgccgccat cgagccgatg aaatcaggcc tggccgacat
      541 tgcctatcag gccgcgctga gacccatgca acggcaggcg gtgccggcat gacgatctcg
      601 gtttcatata aattcgatga agcggtcgtg ctgatcaccg gcgccggcag tggcatcggc
      661 gccgcgctgg cgcgccattg cgcccgcgag ggcgcgtccg tcatcgccat cgaccgcgag
      721 catgacaatc tgctgcgcga tctcgatccg gccctgcgca aaaaaatcga tgtgcgccag
      781 gtcgatgtgt ccaatgaatc ggccgtgacc ggcctgctcg acgacgtgcg gttcaaatat
      841 ccgcgacttg atgtcgcagt tctcgccgcc gccatccagg tccgtaccaa tatcgacgac
      901 atgacagcga gcgagtggcg ctcggtgatc gacaccaatg tcaacggcat cttttactgc
      961 ctgcacgcca taatcccgct gatgaaggcc cagcgatccg gggcgatcgt cgcattcacc
     1021 tcggggctgg cgctgatcgg gtggccgggc gccagcgcct atgcggcaag caaggcggcg
     1081 ctgatcggaa tgatcaagtc ggcggcgcag gagctccgaa actacaacgt gcgggcgaat
     1141 gtgctttcgc ccggggttcg cgctacaccg attttcatgg atgtgagcac ggagacggag
     1201 cgcgagcatt atcgcaactc gatcggcatc ggcgagccgg aaggcgtcgt cccgacgctg
     1261 ctctatctga tctccgatgc ttcagcgaat ctcactggcg ccgtgctcga gcaccggttc
     1321 accccggtga acgagcagac gcagtgaaac tgtgcaagtg actaacctgt gacgaacctg
     1381 cgatgaattt ggcggcaaag gcaacttgag aggagactga gatgaagaaa gtgattgcgg
     1441 cgttcctttg ctccgcctgg ctggtgcttg gtggggtaat tggcgggtct gggcaggcgc
     1501 aggcccagga gaaaaaactg accgatatta cgttctccct tgatttcatc acgctgggac
     1561 ggcacgcgcc gttctatgtc gcgatcgaca agggcttcta caaggaagaa ggcctgaacg
     1621 ttaacatcgt gccggccaag ggcacggcac aaggcataca gaacgtcgag tccggcattg
     1681 cgcaattggg cttcgtcgac gtggcgagtc tcgtcgtggc acgcgcggaa ggctcgaccg
     1741 tcaaggtcgt gtccgtgatc taccagaagg cgccgttctg cttcttctcg ctcgacccgg
     1801 gcgccaacgt caccaagctc aaggacttcg agggactgaa ggtcggctcc aacaccggca
     1861 gctacatcac aaacatcgca cgcgcgatga tgcgcaaggc aggccttgat ccgaacaagc
     1921 tgcagatgga aagcatcgag ccgtcggcgc gcatcgccat gctggcgacg cgcaagattc
     1981 cggcgatcga cttcttcatc atcaccaaac cg
//
LOCUS       DASSRL010000371         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_604619, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000371 DASSRL010000000
VERSION     DASSRL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604619"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFL49_07515"
     CDS             complement(<1..605)
                     /locus_tag="VFL49_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7911862.1"
                     /translation="MKDILDRLEKRREEARLGGGKARIEAQHKRGKLTARERIELILD
                     KGSFEEFDMFVEHRSNEFGMEKSKIPGDGVVTGWGTVNGRAVYLFSKDFTVFGGSLSE
                     THAQKIIKVQDMAMRARCPIIGLFDAGGARIQEGVAALGGYGEVFKRNVIASGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVKDTSYMFVTGPDVVKTVT"
     gene            690..1598
                     /locus_tag="VFL49_07520"
     CDS             690..1598
                     /locus_tag="VFL49_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7911863.1"
                     /translation="MAPPPKTAYKPGMSEHPTAAELIQQGLVHHRAGQISLAMDRYTQ
                     VLRNDPQNADALYYIAVVACREGQFQQGVDLARRSLSFRPRQARAHNLIGQALHRLGQ
                     PKDALESFDRALECDVNFADAYGNRANMLSELGRHAEAISSFDRALALNPKSATDWLN
                     RGATLHGIGRIDDAIASYDKAIALDPEFCVTHNNRAHALLDAGRDDEALAAYDRAINL
                     EPKMAEAYLGRAVALKKLARLDDALASVDKAIEMKGDVAKMHEARASLLRDLGRDDDA
                     RAADAKAAELAAKAGGEPGTAPAGGN"
     gene            1664..2191
                     /locus_tag="VFL49_07525"
     CDS             1664..2191
                     /locus_tag="VFL49_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET7911864.1"
                     /translation="MNHASSRGAAPRLATPTDLGANAAKDIAAALNGILADTFALFLK
                     TKNFHWHMTGPHFRDYHLLLDEQSEQIFATIDVLAERVRKIGQPTLRSIGDISRQQRL
                     SDNDAAYVEPQDMLAELRDDNQRVVASMREAHDVCDEHNDVASASFLETFIDDAERRV
                     WFLYEASRKGDPTGH"
     gene            complement(2198..2962)
                     /locus_tag="VFL49_07530"
     CDS             complement(2198..2962)
                     /locus_tag="VFL49_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HET7911865.1"
                     /translation="MRDIFTEIFENQPFDPVEAARRGARPILRKRFYETAHIGEGGEV
                     LLDGKVIKTPSRRTLNAPTQQLADAIAGEWNSQSDVIDPARMPLTRLANAIIDAVSEA
                     AEPVADEIEKYLRSDLVFYRAEAPAGLVERQARAWDPVLAWARDRFNARFVLAQGVMH
                     VTQPRESVEALRAAIPKRPWRLGAVSSITTLTGSGLLAVALAHQALDPAEVWSAAHVD
                     EDWQMSQWGHDELVLQRRAFREAEFRAAARVLTLTA"
     gene            3295..>3845
                     /locus_tag="VFL49_07535"
     CDS             3295..>3845
                     /locus_tag="VFL49_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7911866.1"
                     /translation="MKNTDISAADYFHKVVDCQWACPAHTPVPEYIRLIAHGRYSDAY
                     MINWRSNVFPGILGRTCDRPCEPACRRVRVEKEPVAICRLKRVAADYKDDIRERLPKP
                     AAKKNGKRIALVGGGPASLTVARDLAPLGYQCVVFDQDPKAGGMIRTQIPKFRLPDSV
                     IDEECGYILDLGVEFRGGKRIDS"
BASE COUNT          762 a         1266 c         1143 g          674 t
ORIGIN      
        1 gtcaccgtct tcacgacatc ggggcccgta acaaacatgt agctcgtgtc cttcaccatg
       61 aagatgaagt cagtcatggc cggcgaatag acatcgccgc cggcgcaggg gcccatgatg
      121 acgctgatct gcggaatgac gccggacgca atcacgttgc gcttgaagac ctccccgtaa
      181 ccgccaaggg ctgccacgcc ttcctggatg cgcgcgccgc cggcgtcgaa cagcccgatg
      241 atcgggcagc gcgcccgcat cgccatgtcc tgcactttga tgatcttctg cgcgtgcgtc
      301 tcggacagcg agccgccgaa taccgtgaaa tccttcgaga acaggtagac cgcgcggccg
      361 ttcaccgttc cccagccggt gacgacgccg tcaccgggga tcttcgactt ctccatgccg
      421 aattcgttcg agcgatgctc gacgaacatg tcgaattcct caaaggagcc cttgtcgaga
      481 atgagctcga tgcgctcgcg cgcggtcagc ttgccgcgct tgtgctgagc ttcaatccgc
      541 gccttcccgc cgccgaggcg ggcctcctcg cggcgcttct caagtcggtc gaggatatct
      601 ttcattccgc acgcacccct gcccgtcacg ggtcggaatc tgcccgccag aggcatacca
      661 tgagaggcca gcaccgtcat tggagaggct tggcaccacc cccaaagact gcttacaagc
      721 cgggcatgag cgagcatccg accgccgccg agcttatcca gcagggcctc gtccaccacc
      781 gggccgggca aatctcgctc gcgatggatc gctataccca ggtgctgcgc aacgacccgc
      841 agaatgccga cgcgctgtac tacatcgccg tcgttgcctg tcgtgaaggc cagtttcagc
      901 agggcgtcga ccttgcgcgc cgttcgctgt ccttccgccc ccggcaggca cgcgcgcaca
      961 atctcatcgg ccaggcgctg caccggctgg ggcagcccaa agatgcgctc gaaagcttcg
     1021 acagggcgct cgaatgcgac gtcaattttg ccgatgctta tggcaaccgc gccaatatgt
     1081 tgagcgaact cggccgccac gccgaggcga tatcaagctt cgaccgcgcc ctcgcactca
     1141 accccaaatc ggctaccgac tggctcaacc gcggcgccac tttgcacggc atcggtcgca
     1201 tcgacgacgc cattgcgagc tacgacaagg caatcgcgct cgacccggaa ttctgcgtga
     1261 cacacaacaa tcgcgcccac gcgttgctcg atgccggccg cgatgacgaa gcgcttgcgg
     1321 cctatgatcg ggcgatcaat ctcgagccaa aaatggccga ggcctatctc ggccgcgcgg
     1381 ttgcgctgaa aaagctcgcc cggctggacg acgcccttgc cagtgtcgac aaggcaatcg
     1441 agatgaaggg cgatgtcgca aaaatgcacg aggcgcgcgc gtcgctcttg cgcgatctcg
     1501 gacgcgacga cgatgcgcgc gccgctgatg ccaaggcggc tgagctcgca gcaaaggccg
     1561 gcggtgagcc gggcaccgcg cctgctggtg gaaattagtg cccgcaattg gaactttctg
     1621 cgggttttga tcgtttggcg gggtagtcag aaggaacctt tccatgaacc atgcctcctc
     1681 ccgcggcgcg gcgccacgcc ttgccactcc caccgatctt ggcgccaacg ccgcaaaaga
     1741 cattgcggcc gccctcaacg gcatcctggc cgacaccttt gcgctcttcc tcaagaccaa
     1801 gaatttccac tggcacatga ccgggccgca tttccgcgac taccacctgc tgctcgacga
     1861 gcagtccgag cagatctttg ccacgatcga cgtgctcgcc gagcgtgtcc gcaagatcgg
     1921 acaaccgact ctgcgttcga tcggcgatat ttcgcgccag caaaggctgt ccgacaatga
     1981 tgccgcctat gtcgaaccgc aggacatgtt ggcggaattg cgcgacgaca atcagcgcgt
     2041 ggtcgcgtcg atgcgcgaag cgcacgacgt ctgcgacgag cacaatgatg tggcgtcggc
     2101 aagctttttg gagacattca tcgacgacgc cgaaaggcgg gtgtggtttc tctacgaagc
     2161 aagccgcaag ggcgatccga ccgggcacta gccgcgatca ggccgtcaaa gtcagcacgc
     2221 gtgccgccgc gcggaattcc gcctcacgaa acgcgcggcg ctggagcacg agctcgtcat
     2281 ggccccattg cgacatctgc cagtcctcgt cgacatgcgc cgccgaccag acctcggccg
     2341 gatcgagcgc ctggtgcgca agggcgacgg cgagcagtcc cgagcctgtc agcgtcgtga
     2401 tcgatgaaac ggcgccgagc cgccacggcc gcttgggaat agcagcgcgc aaagcctcga
     2461 cggactcgcg cggctgggtg acatgcatga cgccctgcgc caacacgaag cgcgcgttga
     2521 accggtcgcg cgcccaggcc agaaccggat cccatgcgcg cgcctgtctt tcgaccagac
     2581 cggcaggcgc ctcggcgcga taaaacacca gatcggagcg cagatatttc tcgatctcgt
     2641 cggcaacggg ctcggccgct tcgctcaccg catcgatgat cgcattggca agccgcgtca
     2701 gcggcatgcg cgcaggatcg atgacgtcgc tttgcgaatt ccattcgccg gcgatcgcat
     2761 ccgcaagctg ctgcgtcggg gcattcaggg tgcgtcgtga gggcgttttg atgactttcc
     2821 catcaagcag gacttcgccg ccctcgccga tatgggccgt ttcgtagaac cgcttgcgca
     2881 aaatcggccg tgccccgcgc cgcgccgctt caaccgggtc gaacggctgg ttttcgaata
     2941 tctcggtaaa tatgtcgcgc atcagatgat cgtgattgtg cgccgcaaca atagcgcagc
     3001 caagccggcg aagatcaaaa aaatatcgca gtaactgccg ctggcgcgta ccgaaatcgc
     3061 cctgctctgg tcatcacagt gccgctttca gagagcgaaa aacctatctg acacttgtgg
     3121 ttgatagggt ccaaccatga cggaaggata ggtagccttg cggattggta cccaataata
     3181 tccgccacgg aatcgcgccg ggaaacaagg ccaattgagg acataggccg ccggggctgt
     3241 gacgcgacgc cgtttatact gtgccggagt aacggggcgc gtttcgagga gtcaatgaag
     3301 aataccgaca tttctgccgc ggattacttc cacaaggtcg tggactgcca atgggcttgc
     3361 ccggcgcata cgcctgtacc cgagtatatc cggctgatcg cgcacggccg ctactccgac
     3421 gcctacatga tcaactggcg ctcgaatgtg ttcccgggga tcctcggccg cacatgcgac
     3481 cgtccttgcg aacccgcctg ccggcgcgtc cgtgttgaga aggagccggt ggcgatctgc
     3541 cgcctgaaaa gggtggcggc ggactacaag gatgacattc gcgagcgcct gccgaaaccc
     3601 gcggcgaaaa agaacggaaa acgcatcgcg ctggtcggcg gcggcccggc ttcgctcact
     3661 gtcgcccgcg atctcgcccc gctcggctac caatgcgtgg tcttcgacca ggacccaaaa
     3721 gccggcggca tgatccgcac gcaaattccg aaattccggc ttcccgattc cgtcatcgac
     3781 gaggaatgcg gctacatcct cgacctcggc gtcgaattcc gtggcggcaa gcgcatcgac
     3841 agcat
//
LOCUS       DASSRL010000372         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_219952, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000372 DASSRL010000000
VERSION     DASSRL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219952"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFL49_07540"
     CDS             <1..330
                     /locus_tag="VFL49_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911867.1"
                     /translation="EIGFVTMAVVFVSATLLGFASHAPGGLGVFDAAMLVALWQFDKE
                     SVLAGLLLFRLLYYLIPFALALMILGSREIWLNVNGTRRAAEPAMITSVTEPIRSEFE
                     SEKTDAG"
     gene            333..1547
                     /locus_tag="VFL49_07545"
     CDS             333..1547
                     /locus_tag="VFL49_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7911868.1"
                     /translation="MAINDERRSLPNPTGLATRLRGAWLALLGRAETAAGESTAPAAN
                     RVLGQAQTQLIDQLIGGLPAAAIVLDREGRVIAFNEVAAAIAPALRRGEPALISLRMP
                     ELVDAVRRVTKRREPQRVEFFERVPLDRWFEAFVTPVTLPAGSGAAEIVLMTFNDLTP
                     LRRVEEMRADFVANASHELRTPLAALLGFVETLQGPAKDDPAARDKFLAIMQGQATRM
                     ARLIDDLLSLSRIELNAHLRPGTPLDLAPIVRQVADGLQTLARDRGVKINVALPQDAL
                     VVLGDRDELIRALENLVENALKYGAAGKRVDLTVTRGQTRAAAPEARVAVRDYGPGIA
                     PEHLPRLTERFYRVDVTDSRAQGGTGLGLALVKHVLNRHAGRLEIESTPGTGATFTMH
                     IPLRAGEPPGES"
     gene            1776..>2437
                     /gene="pstS"
                     /locus_tag="VFL49_07550"
     CDS             1776..>2437
                     /gene="pstS"
                     /locus_tag="VFL49_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7911869.1"
                     /translation="MKHWTALAAAGFIAAATFVSANAANISGAGATFPYPIYAKWADA
                     YKKITGNGLNYQSIGSGGGIKQIKAKTVTFGASDAPLSGKDLDEAGLAQFPMVMGGIV
                     PVVNLDGVKPGDLVIDGPTLARIFMGDIKTWDDAALKKLNPNVNLPKQAIAVVHRADG
                     SGTTFNFAYYLAETSADWKSKVGFNTSVEWPVGIGAKGNEGVANNVAQTKGSIGYVEY
                     AYA"
BASE COUNT          455 a          757 c          784 g          441 t
ORIGIN      
        1 gaaatcggct tcgtcacgat ggcggtggtc ttcgtctcgg ccacactgct cggctttgcg
       61 agccatgccc ccggcgggct tggcgtgttc gatgcagcga tgctggtggc gctgtggcaa
      121 ttcgacaaag aatcggtgct cgccggcctg ctcctgttca gattgctcta ctacctcatc
      181 ccgtttgcgc ttgccctgat gatcctgggt tcgcgcgaga tttggctgaa cgtgaacggt
      241 acgcgccgcg cggccgagcc ggcaatgata acgtcggtga cagaaccgat ccgcagcgag
      301 ttcgaatcgg aaaaaaccga cgccggttga tcatggccat caatgacgag cgccgcagcc
      361 ttcccaatcc gaccgggttg gccacacggc tgcgcggcgc ctggctggct ctgcttggcc
      421 gagcggaaac ggcggccggc gaaagcactg ctccggccgc caaccgggtc cttggccaag
      481 cgcagacgca gttgattgat cagctgatcg gcgggttgcc cgccgcggcg atcgtgctcg
      541 atcgcgaagg ccgggtgatc gcgtttaacg aggtggccgc ggcgatcgcg ccggccttgc
      601 gtcgcggcga accggccttg atcagcttgc gtatgccgga actggtcgac gccgtccgcc
      661 gcgtcacgaa gcgtcgggag ccgcagcggg ttgaattttt cgagcgtgtt ccgctcgacc
      721 gctggttcga ggcttttgtg acgccggtga cgctgccggc agggagcgga gcggcggaaa
      781 tcgtgctgat gacgttcaac gatctgaccc cgttgcgccg tgtcgaagaa atgcgcgccg
      841 atttcgttgc caatgcgagc catgagttgc gcacgccact ggcggcgttg cttggctttg
      901 tcgagacgct gcaaggaccc gccaaagacg atccggcagc gcgcgacaaa tttctcgcga
      961 tcatgcaggg gcaggctacc cgcatggccc ggttgatcga tgatctgctg tcgctctcgc
     1021 ggattgagct gaacgcccat ttgcggcccg gcacgccgct tgatctcgcg ccgatcgtgc
     1081 ggcaggtcgc cgacggtctg cagacccttg cacgcgatcg tggcgtcaaa atcaacgtcg
     1141 cgttgccgca ggatgcgctc gtggtgctcg gcgatcgcga tgagctcatc cgagcgctgg
     1201 agaatctggt tgaaaacgcg ctcaaatacg gagcggcagg caagcgtgtc gatcttactg
     1261 tcacgcgggg ccagacacgg gcagcggcgc ccgaggctcg tgtggcggtg cgtgactacg
     1321 ggcctggcat cgcgccggag catcttccgc gcctgaccga acggttttac cgcgtggatg
     1381 tcaccgacag ccgcgcgcaa ggtggaaccg gactgggcct tgcgctggtc aagcatgtgc
     1441 tcaaccgcca cgccggacgg ctggaaatcg aaagcacgcc ggggacgggc gcgaccttca
     1501 caatgcatat tccgctgcgg gccggcgagc cacccggcga atcctagtta attcatcggc
     1561 ttgcactgtc atccaggtgt catcgatgat ttatagaacg gtgacatgcg acgcctatgg
     1621 ctgaccgcgg ctccggacca tcctgcaacg aaccaaaaat gtccgaagtc gtcgtggagc
     1681 gcaacagcgg ctgtccgacc gaccgggcac aaggactggg gtgacggatc ttttggtccg
     1741 cgcgggtcct tggccgcaaa catggagaga atcccgtgaa acactggacc gcattggcag
     1801 ccgccggctt catcgccgcc gccaccttcg tttcggccaa cgccgccaac atttccggcg
     1861 ctggcgctac gttcccgtac ccgatctacg ccaaatgggc ggacgcgtac aaaaaaatca
     1921 ctggcaacgg tctgaactat cagtcgatcg gctcaggcgg cggcatcaag cagatcaagg
     1981 cgaaaacggt gacgttcggc gcaagcgacg cgccgctttc cggcaaggat ctcgacgaag
     2041 ccggactcgc ccagtttccg atggtgatgg gcggcatcgt gccggtcgtg aacctcgacg
     2101 gcgtcaaacc gggcgatctg gtcatcgacg ggccgacgct ggccaggatt ttcatgggcg
     2161 acatcaagac ctgggacgat gctgctctga agaagctcaa tccaaacgtc aacctgccga
     2221 aacaggcgat cgctgtcgtg catcgcgcgg acggctcggg caccacgttc aacttcgcct
     2281 actacctggc cgagaccagc gccgactgga aatccaaagt cggcttcaac acctcggtcg
     2341 agtggcccgt cggcatcggc gccaagggca acgaaggcgt cgccaacaac gtcgcgcaga
     2401 cgaaaggttc gatcggctac gtcgagtatg cctacgc
//
LOCUS       DASSRL010000373         7268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_709525, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000373 DASSRL010000000
VERSION     DASSRL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709525"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFL49_07555"
     CDS             complement(<1..319)
                     /locus_tag="VFL49_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HET7911870.1"
                     /translation="MAIIDVALAQPFGLNRGVPPPDTGGFAGWILAKQAGFYRMLSGL
                     IRAAKADGSAAYTLLGISFLYGVFHAAGPGHGKAVISSYLVANNETWRRGIVLSFASA
                     ILQA"
     gene            complement(382..1023)
                     /locus_tag="VFL49_07560"
     CDS             complement(382..1023)
                     /locus_tag="VFL49_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HET7911871.1"
                     /translation="MKALLHILFAALLGLGATSLAQAHPHVWVTMQTELVYAPDGSIT
                     GIRHAWSFDDMFSTFATQGLESKEKGKFTREELAPLAKVNVESLKEYDYFTYATADGT
                     KAELTDPAPGYWLDYKDQILTLNFLLPLKKPVKAKELKVEIYDPSIFVDFAFAKDKPV
                     QLAGAPKCKFDVVLPREMTFAEGKALSQIPADQKNTTMTWGAQFASKILVHCP"
     gene            complement(1104..2699)
                     /locus_tag="VFL49_07565"
     CDS             complement(1104..2699)
                     /locus_tag="VFL49_07565"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7911872.1"
                     /translation="MTLHSAGHRRGVVAAPHAAAVEDGRAVLAEGGNAIEAMLAMAAS
                     IAAVYPHMNHVGGDGFWLVREPSGRVRALMGAGRAGARATPRLYREAGYDEIPARGPL
                     AALTVPGAVAAWMLAWDAARAQGGKLPLDLLLAPAIKHASGGYVVTPSQARLTAEKLP
                     EMDKAVGFRTAFLLDGKVPEVGARLKQTAFAATLEQLARAGLDDFYRGDIGREIAADL
                     ERIGSPITRADLESYRAIVDEPLSVVTQAGTLYNAPPPTQGLASLIILALFERLRVAR
                     AESFEHIHGIIEATKRAFRVRDQVITDPDKIAADLGRYFMPAFLDSEAAKIDARKAAK
                     WPAPYGEGDTIWMGAADSSGLVVSYIQSLYWEFGSGCVLPATGIVMQNRGASFSLDPK
                     ALNVLAPGRRPFHTLNPALAVLKDKRIIAYGTMGGDGQPQSQAAVFTRHVTFGQPLEK
                     ALDAPRWLLGRTWGSTHTNLRLESRFDGNLVDHLLSAGHDVAVLDEAYSDTMGHAGAV
                     VLHADGTLEGGHDPRADGGAAGV"
     gene            complement(2715..4781)
                     /locus_tag="VFL49_07570"
     CDS             complement(2715..4781)
                     /locus_tag="VFL49_07570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7911873.1"
                     /translation="MPSAENQNPLLSQWAGALELPPFETIRPDHFRTAFDLALADHRT
                     EIDTIAENPAPPDFENTIAALERSGRALERVAGIFFVLAGADTSDEIEAIERDISPLL
                     ARHNNALYLNGPLYSRIADLYARRAALSLDAEQARVLERYHTRFVRSGAALDKRAQDR
                     LAAVNERLATLGTRFGQNVLADEKSFAMVLEEGDLAGLPEFARAAARAAADARGHPGK
                     YVVTLARSSVESFLQFSSRRDLREKALQAWISRGENGGQTDNRALIAEMVALRAERAK
                     LLGFANFADYRLDDQMAKTPQAVRKLLGDVWGRALTKAAAERDALQAMISEEGGNFAL
                     APHDWRYYAEKLRKARYDLDEAEIKPYFQLEKMIEAAFETAHRLFGLSFEPVDAVLYH
                     PDARAWNVTDAKGDHVALFIGDYFARPSKHSGAWMTSLRDQEKLAGNIRPIVLNICNF
                     SKPADGEAALLSFDDARTLFHEFGHALHGMLSDVTYPLLAGTAVPSDFVELPSQLYEH
                     WLEVPEILQKYALHAHTGQRMPKALLDRLLATRTFNQGFATVEYTACALVDLDLHALP
                     DATALDVAAFERDDLARIAMPPEIVMRHRLPHFQHLFSGGGYAAGYYSYMWSEVLDAD
                     AFAAFEETGNVFDPVMARRLHDYVYSAGNRRDPTEAYKAFRGRLPTVDALLKKRGLVE
                     TGVGAH"
     gene            complement(4858..6696)
                     /locus_tag="VFL49_07575"
     CDS             complement(4858..6696)
                     /locus_tag="VFL49_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7911874.1"
                     /translation="MDVLNALKIARSLLAGFAALGLLAGIMAKLVGWPGVATAVWTAA
                     TLPVLLALLVEIVTSLRRGEIGLDIVAGLSMATALIFGENLAAAIVALMYAGGQYLEA
                     YAEKTARREMTALLSRVPRTAIRHRDGQLEEIAIDLVLPGDRLLVRRGDVVPVDGVVA
                     DGPAVLDQSALTGESLPVKAKQGDALLSGSTNAGEPFGMIATRLAAESTYAGIVRLVE
                     DAQRSRAPMSRLADRYAILFLALTVILAGGAWWLTGDPIRAVAVLVVATPCPLILAVP
                     VAVVSGLSRAAKFGILIKGGKAIEILARVRTVVIDKTGTLTVGRARIIATRMNGGISA
                     DELLRITASLDQASKHVIAQTIVEEARNKGLQLTVPADVVETPGEGLVGRVEDHIVAV
                     GGLHFVARKVAKSDVLPLLPDRAPGALAVAVAIDGQLSGVLILADKVRAGTEVMLREL
                     RGMGIERIVLATGDRHKVAEFVATGLSLDLVRSELTPDQKILVVLAERKNGPVMMIGD
                     GVNDAPALAAADVGVAMGANGAAASAQAADVVLLVDQLDRILPAIRIARRSRLIALQS
                     VCAGIGLSAAAMIAASFGLLAPAEGAVIQEVIDVAVIFNALRALHG"
     gene            6825..7067
                     /locus_tag="VFL49_07580"
     CDS             6825..7067
                     /locus_tag="VFL49_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911875.1"
                     /translation="MAQNRRIVWLNVITVISAAILIGAEVFGAAFAGSWAIATLFDLG
                     GIGARVLDVLFVLCGFVIMAQFIRTAHRIEPFTAHD"
BASE COUNT         1464 a         2306 c         2212 g         1286 t
ORIGIN      
        1 atgcctggag aatggcggaa gcaaacgaca atacgatccc gcgccgccag gtttcgttgt
       61 tggcgacgag gtaagatgag atcactgctt tgccgtgccc cggccccgct gcgtgaaaca
      121 cgccgtacag aaatgagatg ccgagcaacg tgtaagcggc gctgccgtcc gccttggcgg
      181 cgcggatcag gccggacagc atgcgataga acccggcctg ctttgcaaga atccagccgg
      241 caaagccgcc cgtgtctggc ggcgggacgc ctcgattgag cccgaaaggc tgtgccagcg
      301 ccacatcgat aatggccaga aaaagcaata tggctgcggt cgccaaaatc ccgcgcaagg
      361 cgcgcgttcg cgaggcagcg ctcaaggaca atgcaccagg atcttgctgg cgaattgtgc
      421 gccccaggtc atcgtggtgt ttttctggtc cgcaggaatc tgactgagcg ccttgccctc
      481 ggcaaaggtc atctcacgcg gcaacacgac gtcgaacttg cacttcggcg cgccggcaag
      541 ctgcaccggc ttgtctttgg cgaatgcaaa gtccacaaag atggaaggat catagatttc
      601 gactttcagt tcctttgcct tgaccggctt cttgagcggc agcaggaaat tgagcgtgag
      661 gatctggtcc ttgtaatcaa gccagtaacc cggcgcagga tcggtgagct ccgccttcgt
      721 gccgtcggcc gtggcataag tgaagtagtc gtactccttc agcgactcga cattgacctt
      781 ggcaagcggc gcgagctctt cgcgcgtgaa tttgcccttc tccttgctct ccagcccttg
      841 ggtcgcaaag gtggaaaaca tgtcgtcgaa cgaccaggcg tgacgaattc ccgtgatgct
      901 gccgtcgggc gcatagacga gttcggtctg catcgtcacc cagacgtgcg ggtgcgcctg
      961 cgcgaggctg gtggcgccaa gaccgagcag tgcagcaaaa agaatgtgaa gcagcgcttt
     1021 catgcgggcg cacgatagca cggactgcgg cgcaaaggtg gcgtgtaacg atgttacgaa
     1081 agctttagcg taaacgcggc atatcaaacc cccgccgctc cgccgtcggc acgcggatca
     1141 tgaccgcctt ccaaggtacc gtcggcgtgc aacaccacgg ctcccgcgtg gcccatcgtg
     1201 tcggagtaag cctcgtcgag aacggccaca tcgtgtccgg ctgacaaaag atgatcgaca
     1261 agatttccgt cgaaacgcga ctccagccgc agattggtgt gggtcgagcc ccaggtccgg
     1321 cccagcagcc aacgcggcgc atcgagggcc ttctcaagcg gctgcccgaa tgtgacgtgg
     1381 cgcgtgaaca ccgccgcctg gctctgcggc tggccgtcgc cccccatcgt gccgtaggcg
     1441 atgatccgct tgtccttgag aacggcgagc gcgggattaa gcgtgtgaaa tggacggcgg
     1501 ccgggcgcca gcacattcag cgcctttgga tcgagggaga agctcgcgcc gcggttttgc
     1561 atgacgatac cggtcgccgg cagcacgcag ccggagccga actcccaata aagcgactgg
     1621 atgtacgaga cgacgaggcc gctcgaatcc gccgcgccca tccagatcgt gtcgccctcg
     1681 ccgtagggcg ccggccactt tgccgccttg cgcgcatcga tcttggccgc ctcgctatcg
     1741 aggaatgccg gcataaaata ccggccgaga tccgccgcga tcttgtccgg atccgtgatc
     1801 acctgatcac gcacacggaa ggcgcgcttg gtggcttcga tgatgccatg gatatgttcg
     1861 aagctttcgg cccgagcgac acgaagtcgc tcgaacaacg caaggatgat cagcgatgcg
     1921 aggccctgcg tcggaggggg agcgttatag agggtgcccg cttgcgtcac gacgctgagt
     1981 ggctcatcca caatcgcgcg atagctttcc aggtcggcgc gcgtgatcgg gctgccgatg
     2041 cgctcaagat cggccgcgat ctcgcgcccg atatcgcccc ggtaaaaatc gtcgagtccg
     2101 gcgcgggcga gttgctcaag cgtcgcagca aaagctgtct gcttcaagcg cgcgccaacc
     2161 tctgggacct tgccgtcgag cagaaatgcc gtcctgaagc cgacagcctt gtccatctcc
     2221 gggagttttt cggccgtcag tcgcgcctgg ctgggtgtga caacgtatcc gccgctcgcg
     2281 tgtttgatag ccggcgcaag caacaggtcg agaggcagct tgccgccctg cgctcttgcg
     2341 gcgtcccatg caagcatcca cgcagccact gcacctggaa cggtcagtgc ggcaagcggg
     2401 ccgcgggccg gaatttcatc gtaaccagcc tcacgataaa ggcgcggcgt ggccctggcg
     2461 cctgcacgcc cggcgcccat cagcgcccgc acgcggcctg acggttcgcg caccagccag
     2521 aagccgtcgc cgccgacgtg gttcatgtgc ggatagaccg ccgcgattga agcggccatg
     2581 gcgagcatcg cttcgatggc attaccgcct tcggcgagca ccgcgcgtcc atcctcaacg
     2641 gcggcggcat gcggtgcggc cacgacgccg cgccgatggc ccgcgctatg taacgtcatt
     2701 gatcccccga acgttcaatg cgcaccaaca ccagtttcga caaggccccg ctttttcagc
     2761 aaggcatcaa cggtcggcag tcggccgcgg aaggccttgt aggcctcggt aggatcacgg
     2821 cggtttcccg cgctgtaaac atagtcgtgc aggcgccttg ccattaccgg atcgaaaaca
     2881 ttgcccgttt cctcgaacgc cgcgaaggcg tcggcatcga gtacttccga ccacatgtac
     2941 gaataatagc cggctgcata ccccccgccg gagaacaagt gctgaaaatg cggaagccgg
     3001 tgccgcataa cgatttccgg cggcatcgcg atgcgcgcga gatcgtcgcg ctcgaatgcc
     3061 gcgacatcga gcgcggtcgc atccggcagc gcgtgcaggt cgaggtcgac cagcgcgcaa
     3121 gccgtatact cgaccgtcgc aaaaccctga ttgaaagtgc gcgtcgccaa caagcgatcg
     3181 agcaaagcct tcggcatccg ctgaccggta tgcgcgtgca acgcatattt ctgcagaatc
     3241 tccggcacct ccagccaatg ctcgtagagt tgcgagggta actcgacgaa gtcgctgggg
     3301 acggccgtgc cagcgagaag gggataggtc acgtcggaca acatcccgtg caacgcatgc
     3361 ccgaactcgt gaaagagcgt gcgggcgtcg tcgaacgaca gcagcgcagc ctcgccgtct
     3421 gccggctttg aaaaattgca gatattgaga acaatcggcc ggatattgcc agcgagcttc
     3481 tcctgatcgc gcaaggacgt catccaggca ccgctgtgct tggatggccg cgcgaagtaa
     3541 tcgccaatga acaacgcgac atgatcccct ttggcatccg tcacattcca ggcacgagca
     3601 tccgggtgat aaagaaccgc atcgacgggc tcgaacgaca gcccgaacaa ccggtgggcg
     3661 gtctcaaacg ccgcctcgat cattttctca agctggaaat acggcttgat ctcggcctcg
     3721 tcgagatcgt aacgcgcctt gcggagcttc tctgcgtagt agcgccagtc gtgcggcgcg
     3781 agcgcgaaat tgccgccttc ctcggaaatc atcgcctgca gcgcgtcacg ctcggccgcc
     3841 gccttggtga gcgctctgcc ccagacgtca ccaagcagct tgcgaaccgc ctgcggcgtc
     3901 ttggccatct ggtcgtcgag acgatagtcg gcgaagttcg cgaagccgag cagcttcgcg
     3961 cgttctgcgc gtagcgccac catttcggcg atcagagccc gattgtccgt ttgtccgccg
     4021 ttttccccgc gggagatcca ggcctgcaat gccttttccc gcagatcacg ccgtgaagag
     4081 aactggagaa agctttcaac agacgatcgt gccagagtaa caacatattt gcccggatgg
     4141 ccgcgcgcat cggcagcggc acgcgcagcc gcgcgggcaa actccggcag tccggcaagg
     4201 tcaccctctt cgaggaccat cgcgaaggat ttctcgtccg ccaggacatt ctggccaaac
     4261 cgggtcccaa gagtcgccag gcgctcgttg accgccgcaa gccgatcctg cgcgcgctta
     4321 tccaacgccg ccccggagcg gacgaaccgc gtatgatagc gctccagcac gcgggcttgc
     4381 tccgcatcga gcgaaagcgc ggcacgcctg gcgtaaaggt cagcgattcg ggaataaagc
     4441 gggccgttca gataaagcgc gttattgtga cgcgccaata gcggcgagat gtcccgctcg
     4501 atggcttcga tttcgtccga cgtatcggcg ccggccagca caaagaatat gcccgcgacg
     4561 cgctcgagag cacggccgct tcgctcgagt gcggcgatgg tgttttcaaa gtccggaggc
     4621 gccggatttt cggcaattgt gtcgatctcg gtgcggtgat cggccaaggc caggtcgaat
     4681 gccgtacgga aatggtcggg cctgatcgtc tcgaatggcg ggagttcgag tgctccagcc
     4741 cattgagaaa gaagcgggtt ctgattttct gctgaaggca ttcgcgaggt gttcatgcgg
     4801 cctatatagg gggcttagca gatgcgggga gagatgccat agcagcagcc tggcgaatca
     4861 gccatgcagt gccctgaggg cattgaagat aaccgccaca tcgatcactt cctgaattac
     4921 cgctccctcg gccggggcga gaagcccgaa tgatgcggcg atcattgccg cggctgaaag
     4981 ccctatgccc gcgcacacgc tttgcaacgc aatcaagcgt gaacggcgcg cgattctgat
     5041 tgcaggcaga atacggtcga gttgatcgac cagcagcacc acatcggctg cctgggcgga
     5101 ggcagcggct ccgttggccc ccatcgccac acccacatcg gcggcagcaa gagccggcgc
     5161 gtcattcact ccatcgccga tcatcatcac cgggccgttc ttgcgctctg ccaataccac
     5221 cagaattttc tgatcgggag tgagttcgga gcggacgaga tcgagggaaa gaccggtggc
     5281 aacgaactcg gcgactttgt gccggtcgcc ggtcgcgaga acaatgcgct cgatgcccat
     5341 cccgcgcagt tcacgcagca tgacttcagt acccgcacgc accttgtccg caaggatcag
     5401 cacgcccgac aattgcccgt cgattgccac cgcgaccgcg agcgctcccg gagcgcgatc
     5461 aggcagtagc ggcaggacat cgcttttggc gaccttccgc gcgacaaaat gaaggccgcc
     5521 gacagcgaca atgtggtctt ccacccgacc gacgaggcct tcaccgggtg tttccacgac
     5581 gtcggcaggc accgtcagtt gcagtccctt gttccgggct tcctcgacga tcgtttgcgc
     5641 gatcacgtgc ttggaagcct ggtcgaggga ggcagtgatc cgcaagagct catccgcgct
     5701 gatgccgcca ttcatccgcg tcgcaataat tctggcccga cccacggtga gcgttccggt
     5761 cttgtcgatg acgacggtgc gcactcgcgc gaggatttcg atggccttgc cgcccttgat
     5821 aaggattccg aacttcgcgg cgcgggaaag tcccgagacg acagctaccg gcacggcaag
     5881 aatcagcgga caaggcgttg ccacaaccag aaccgcgact gcccggatcg gatcaccggt
     5941 gagccaccag gcgccaccgg caaggatgac ggtgagtgcc aggaacagga tcgcgtaccg
     6001 gtccgccagg cgcgacatcg gcgcccgcga gcgctgagca tcttcgacaa gccgcacaat
     6061 tcccgcatag gtgctctcag ctgccaaccg cgtggcgatc atgccaaacg gctcgccggc
     6121 attcgtggag ccgctgagaa gcgcatcacc ttgcttcgcc ttgacgggca gcgactctcc
     6181 ggtaagggcg gactggtcga gcactgcagg cccgtcggcg acgacgccgt cgacaggcac
     6241 gacgtcacct cgccgcacca acagccggtc accaggcaaa acaaggtcga tggcaatctc
     6301 ttccagctgc ccatcccggt gccgaatcgc cgtgcgcgga acccgcgaca gaagggccgt
     6361 catttcccgc cgcgcggtct tctcggcata ggcctcgaga tattgtccgc cggcgtacat
     6421 cagagccacg atggcggctg caaggttctc gccgaagatc aacgccgtcg ccatcgaaag
     6481 tccggcaacg atgtcgagcc cgatctcccc ccgccgcagg ctggtcacaa tttcgaccag
     6541 caacgccagc aacaccggca acgtggctgc ggtccaaaca gcggttgcca cgccgggcca
     6601 accgacaagc ttggccatga tgcccgccag aaggccaagc gcggcgaaac cggcgagcaa
     6661 tgaccgggca atcttgagcg cgttgagaac gtccatggtt aatcttttgg ccgaccggca
     6721 gcggccgcca tgagaaaagt caagctcgtg cattgagcga ggggcgctat agacttgatc
     6781 ccgcaaccgg tttcggtgac attgcgccac caaaggaagg ccccatggct caaaaccgcc
     6841 gcatcgtctg gcttaacgtc atcaccgtga tcagcgcggc catcctgatc ggcgccgagg
     6901 tcttcggcgc agccttcgcc ggctcctggg cgatcgccac attgttcgat ctcggcggca
     6961 tcggcgcgcg cgtgctcgac gtgctgttcg tgctctgcgg ctttgtcatc atggcgcaat
     7021 tcatccgcac cgcgcaccgc atcgagccat tcaccgctca cgactgagtg cggcctcgtc
     7081 aggctccgaa agaaaaagtt aagaaagttt tttgatgcag cgaactaatt cgcttgcatg
     7141 actcggcaaa attacttatc tccgttgtgc ccaatttcgc acgtgcctgt ggccgtgtgt
     7201 cggtcggtag gtagcgggac aagaggcccg ccagccgcct ggagagagaa gccaaaacct
     7261 ctccaaga
//
LOCUS       DASSRL010000374         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_394861, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000374 DASSRL010000000
VERSION     DASSRL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394861"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2133)
                     /locus_tag="VFL49_07585"
     CDS             complement(<1..2133)
                     /locus_tag="VFL49_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7911876.1"
                     /translation="MRTLRRWFWLWILSVFPTGPAWAAEGAKGPSEAVFIVEIIALMV
                     VGRLLGEAMLRLRQPAVMGQLIAGLLLGPSFFGLLLPDAQHALFPKTPEQKAMIDAIS
                     QFGILLLLLLTGMETDLKLVRQTGRASIFASLMGIVIPFLCGVGLGEMLPDSMLPDPD
                     KRLITSLFMGTALSIASVKIVAMVVREMNFMRRVVGQVILASAIIDDSIGWIIVSIIF
                     SLALHGSVETVSLAQSVIGTIAFMVASLTVGRRAVFFVIRWVNDNFVSEFAVITAILV
                     IMGTMALITYLIGVHTVLGAFVAGILVGESPILTRHIDEQLRGIITAFFAPVFFGIAG
                     LTADLTILSDPKIALFTVGLILIASIGKFTGAFVGAELGGLTRREGFALACGMNARGS
                     TEVIIATVGLSMGALNQDLFTMIVAMAVLTTMAMPPTLRWALGRIPMRQEEKQRLERE
                     ELEAKGFVPNLERLLIAVDDSPNGQFASRVAGVLAGTHGMPATVLHIPDAAKIEDTMP
                     DDADGVDAQKARKAEDAKSSDTKSEKPKKKAEKDEQKAEKAGDTVQQAAAQIKSKQKK
                     EEKVDAPVEVTTIVHEQPSPATIAAEAKKGYDAFIIGLEKTSKRSAEFDQGVTNLARG
                     FEGPLIITAVRNDLLKNPVGKLSVLVPVNGTEPSRRAAEVAITMARATRAPVTVLYVA
                     ARTGSRRGARNRRHEEAILKDVVAIADGY"
     gene            2374..3705
                     /locus_tag="VFL49_07590"
     CDS             2374..3705
                     /locus_tag="VFL49_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7911877.1"
                     /translation="MTDVSGWRRERVRPPLAEVFGSIATRPTGSLGKKLIAFLGPGYL
                     VAVGYMDPGNWATSLAGGSKFGYALLTIALLSNLMAILLQALCARLGIATGRDLAQAC
                     RDAFPRPVSWPLWVLAEVAICATDLAEVIGTAIGLNLLFHIPLEIGVLLTALDVFLIL
                     WLQNLGFRYVEAFVVTLLGVIAACFAVQIAMADPDWGAVIRGFAPTTEVVTNPDMLYL
                     ALGILGATVMPHNLYLHSGVVQTRRFGSSVGDRSEAIKLATIDSTIALMFALLINASI
                     LILAAATFNKVGKTDVAELDQAHSFLAPLLGSAIAPTLFGIALLCCGMNSTVTATLSG
                     QIVMEGFLDIRLPGWARRLITRAIAIVPAAIVTIWYGEKGSAQLLILSQVILSLQLPF
                     AIVPLVMFTADRRKMGDLAAPRWVTALAAITAVTIIALNIKLLVDFALARG"
     gene            complement(3711..4460)
                     /locus_tag="VFL49_07595"
     CDS             complement(3711..4460)
                     /locus_tag="VFL49_07595"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HET7911878.1"
                     /translation="MTQHSRDTPQFYLTAPSPCPYLEGQEERKVFTHLVGERAGELND
                     LLTHGGFRRSQSIAYRPACEGCRACVSVRVVAADFAPSRNMRRIAERNADMVGHMRSP
                     APTSEQYSVFRAYLDARHRDGGMADMTVLDYAMMIEDSHVDTRVIEYRRRQPHLGVSG
                     RNTGQVLAVALTDVLSDGLSMVYSFFEPDEGDRSLGTFMILDHIERAKRMDLPYVYLG
                     YWVPGSRKMDYKGRFLPQERLMPSGWSRVEK"
     gene            complement(4522..>4885)
                     /locus_tag="VFL49_07600"
     CDS             complement(4522..>4885)
                     /locus_tag="VFL49_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7911879.1"
                     /translation="LGLGFALYWLLSPATVIWALLYFGGTLGGPNSATIGMRVMDLEM
                     RTWYGAPAYFVLGAVHAIAFWFTISFFTPFVLLVAFFNHRRRLLHDIMLGTVVINNAA
                     RASMLRGSTRRPAPAEGL"
BASE COUNT          959 a         1433 c         1470 g         1023 t
ORIGIN      
        1 gtagccgtcg gcgatggcaa caacatcttt caggatcgcc tcttcgtgcc gacgattgcg
       61 agctccgcgg cggctgcccg ttcgtgctgc cacatatagg accgtgaccg gtgctctggt
      121 cgcccgcgcc atcgtaattg ccacttcggc ggcacgcctc gacggttcgg tgccgttgac
      181 cggtacaaga acgctgagct ttccgacggg atttttcagt aaatcgttcc gcacggcggt
      241 gatgatcaag ggaccttcga agcctctggc cagattggtg acgccctggt cgaactccgc
      301 gctgcgcttc gatgttttct ccagcccaat gatgaaagcg tcgtagccct tttttgcctc
      361 agcagcgatg gtggccgggc ttggctgctc gtgcacgatc gtcgtcacct cgacgggtgc
      421 atcgactttc tcctcctttt tctgcttgct tttgatttgg gcagcggctt gctgaaccgt
      481 atcgccggcc ttttccgctt tttgctcgtc cttttccgcc ttcttcttgg gtttttccga
      541 cttcgtatcg ctgcttttcg cgtcctccgc cttccttgcc ttctgcgcgt ctactccgtc
      601 cgcatcgtcc ggcatggtgt cttcgatctt ggccgcgtcg ggaatgtgca ggaccgtcgc
      661 cggcattccg tgggtgccgg ccagcacacc ggcaactcgc gatgcgaatt gcccgttggg
      721 gctgtcgtca accgcgatga gcaggcgctc gagattggga acgaagcctt ttgcctcaag
      781 ttcctctcgc tcaagtcgct gcttctcctc ttggcgcatc gggatgcggc cgagcgccca
      841 tcgcagtgtc ggcggcatgg ccatggtggt gaggacggcc atcgccacaa tcatggtgaa
      901 cagatcttga tttagtgcgc ccatcgacaa cccgacggtg gcaatgatca cttctgtcga
      961 gccgcgcgca ttcatcccgc aagcgagtgc gaaaccctcg cgtctggtca ggccgcccag
     1021 ttcagcgccg acaaatgctc cagtgaattt tccgatgctg gcaatgagga tgagaccaac
     1081 ggtaaacaag gcaattttcg ggtcactcag aattgtgaga tctgcggtca atccggcgat
     1141 gccaaaaaaa acgggggcga agaacgccgt gatgataccg cgcaactgtt cgtcaatgtg
     1201 acgcgtgagg atcggcgatt cgccgaccag aattcccgcg acgaatgcgc cgagaacagt
     1261 gtgcacgccg atcaaatagg tgatgagggc catagtgccc ataatgacaa gtatggctgt
     1321 gatcaccgca aattcgctga cgaaattgtc gttcacccag cggataacga aaaataccgc
     1381 gcggcggccg accgtcaggc tcgccaccat gaacgctatc gtgccgatga cgctttgtgc
     1441 caacgagacg gtttcgacag agccgtgcag ggccagactg aaaatgattg aaacaatgat
     1501 ccagccgatg ctgtcgtcga tgatggcgga tgcgagaatg acctggccga cgacgcgacg
     1561 cataaaattc atttcgcgca ctaccatcgc aacgatcttc accgaggcga tcgacagagc
     1621 cgttcccata aaaagggatg tgatcaatcg tttgtccggg tccggcagca tgctgtccgg
     1681 caacatttca ccgagcccga cgccgcacag gaatggaatg acgatgccca tcagcgaggc
     1741 aaagatggag gcccgtcccg tctgccgcac aagcttgagg tcggtctcca tgccggtgag
     1801 caaaagcagc aacaggatgc cgaattgcga aattgcgtcg atcatcgcct tctgttcagg
     1861 ggtcttggga aagagtgcat gctgcgcgtc tggaagaagg aggccgaaga acgacggtcc
     1921 aagcagcagg ccggcgatca attggcccat gacggcaggc tggcgcagcc gcagcatggc
     1981 ttcgcccagc agccggccca caaccatgag cgcaatgatc tcgacgatga acacggcctc
     2041 cgaagggccc tttgcgccct cggcagccca ggccggcccg gtcgggaaga ccgataggat
     2101 ccacagccaa aaccagcggc gaagcgtcct cataagctcg gcaaagctcc aggcagccgt
     2161 cctgcaagac cgacaggacg cggttcatgt ggcggcttga aaacagcgct tgcgccgccg
     2221 ttggggactg caaaccccgg acaaccaaac aggattccaa aggttccggc gagaaatgca
     2281 attgcaaatt attcgcaata agctatctta tcagaatcgg taatttggct tttgcagagc
     2341 ttgctgatgg atgcgaggac gcggctgtcg tcaatgactg atgtatctgg ctggaggcgc
     2401 gaacgcgtcc ggccgccgtt ggccgaagtt ttcggttcga tagccacgcg tcccaccggc
     2461 tcgcttggca aaaaactcat agcttttctt ggtccgggtt acctcgtcgc tgtcggctac
     2521 atggacccgg gcaactgggc gacctcgctg gctggtggct ccaaattcgg ttacgcgctg
     2581 ctcacgatcg cgttgctttc gaacttgatg gcgatcctgc tgcaggcgct ttgtgcgcgc
     2641 ctcggcattg cgaccggacg cgatcttgct caagcctgcc gtgatgcgtt cccgcgcccc
     2701 gtgtcatggc cgctttgggt tttggcggaa gttgcgatct gcgccaccga tctcgctgag
     2761 gtgatcggca ccgcgatcgg cctgaacttg ttgtttcaca ttccgctcga gatcggtgtc
     2821 ctgctgaccg cactcgacgt ttttcttatt ctgtggctgc aaaatctcgg cttccgctac
     2881 gtggaagcgt ttgtcgtgac gttgcttggc gtcatcgcgg cttgttttgc agtccagatc
     2941 gcaatggccg atccggactg gggcgccgtt attcgtggct ttgccccgac gacggaggtc
     3001 gtgaccaatc cggatatgct ctatcttgcg ctcggaattc ttggcgcaac ggtgatgccg
     3061 cacaacctct atctccactc aggagtggtg cagactcgcc gtttcgggag cagtgtcggt
     3121 gaccggagcg aggcaatcaa acttgcgacc atcgattcca ccatcgcgtt gatgttcgcg
     3181 ctgttgatca atgcctcgat cctcatcctt gcggcggcga ccttcaataa agttggcaag
     3241 acggatgtag ccgaacttga ccaggctcat tcgtttcttg cgccgctttt gggctcggcg
     3301 atcgcgccga cattgttcgg catcgcgttg ctttgttgcg gtatgaattc gacggtgacc
     3361 gcaacactgt cgggccagat cgtgatggag gggttccttg acatccggct gcccgggtgg
     3421 gcgcgccgcc tgatcacgcg cgcaatcgcc attgtgcccg cggcgatcgt caccatctgg
     3481 tatggcgaaa aaggcagcgc gcagttgctg atcctgtcgc aggttatatt gagcctgcaa
     3541 ttgccgttcg cgatcgtgcc gcttgtgatg ttcaccgcag atcgcagaaa aatgggcgat
     3601 ctagctgcgc cgcgatgggt caccgcgctg gccgcgatca cggcggtaac tatcatcgcg
     3661 ctgaatatca aactgctcgt ggatttcgcg ctggcaaggg gctgaccagg ttatttttcg
     3721 acccggctcc agcccgacgg catcaggcgt tcctgcggca gaaagcggcc cttgtagtcc
     3781 atcttgcgcg agccgggcac ccaatagccc agatagacgt acggcaggtc catccgtttt
     3841 gcgcgctcaa tgtgatcgag aatcatgaaa gtcccgagcg accggtcacc ttcgtccggt
     3901 tcgaaaaagg aatagaccat agacaggcca tcgctgagca cgtcggtgag ggcgacggcg
     3961 agcacctgac cggtattgcg tccactgacg ccaagatgag gctggcgccg acgatactcg
     4021 ataacacgcg tatcgacatg gctgtcttcg atcatcattg cgtaatcgag cacggtcatg
     4081 tcggccatgc cgccatcgcg gtgacgggcg tcgaggtacg cccggaagac cgaatactgt
     4141 tcggatgtcg gtgccggcga gcgcatgtgg ccgaccatat ccgcgttgcg ctcggcgatc
     4201 cggcgcatgt tgcgtgacgg ggcaaaatcc gcagcgacca cgcggaccga cacacaagcc
     4261 cggcaaccct cgcaggcggg ccgataagca atggattgac tgcggcggaa gccgccgtga
     4321 gtgaggagat cgttgagctc gccggcgcgc tccccgacga ggtgggtaaa caccttacgc
     4381 tcttcctgac cctccagata cgggcaaggc gagggtgctg taaggtaaaa ctgcggcgtg
     4441 tcgcgcgaat gctgggtcac tggtcagacc gagtccggtg gttctggatt cgccctagca
     4501 ttgcgcgccg gaggttctgc gtcaaagccc ctcggcgggc gccggcctcc gcgtggatcc
     4561 ccgtaacatg gaggcccggg cggcgttgtt gatgacgacg gtgccgagca tgatgtcgtg
     4621 caggagccgt cggcggtgat tgaagaaggc gaccagcagc acaaaaggtg tgaagaatga
     4681 aatcgtgaac cagaacgcga tggcgtgcac tgcgccgagc acgaaataag ccggagcgcc
     4741 gtaccaggtg cgcatttcga ggtccatcac ccgcatcccg atagttgcag agtttggtcc
     4801 tccgagggtg ccgccgaaat aaagcaacgc ccagatgacc gtcgccggcg agagcagcca
     4861 atagagggcg aagccaagcc caagc
//
LOCUS       DASSRL010000375         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1671948, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000375 DASSRL010000000
VERSION     DASSRL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671948"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFL49_07605"
     CDS             complement(<1..671)
                     /locus_tag="VFL49_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911880.1"
                     /translation="MKNRLARAMGIFLIPIASAAFAIDAGTAKGTLKADGKDVPLKYS
                     VAYQLDNAEGLLDRPKEMRILIADREVPAEALAGLAFPPVMQMARENKVQGILLRFDP
                     ADRKAVLVTWLAAPKDPRMSLVNLTVSGTQDAFRKLDVGSTRVIGEIAYKDSRPGSSD
                     MPSLEFAINFSSPVFHNPAVTADLKGEAARKSPQAQLLRDKARALAKADFAAVKKLST
                     ERQNKS"
     gene            complement(734..1690)
                     /locus_tag="VFL49_07610"
     CDS             complement(734..1690)
                     /locus_tag="VFL49_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7911881.1"
                     /translation="MIRSRMIPFAVLALLSASSFAQPYPGKPVRVITPWPSGGLTDVA
                     GRIVFQKVSENLGQQFLVDNRPGATGSIGADLVAKSPPDGYTLMVHSMAHLGNPYIYP
                     KLPYDTLGDFIGIGTLVRQTGLLVVHPSLPVKSVKELVALAKARPDQILYATSGSGSF
                     SHFAFVLLASMTNTKLVHVPYKGGGPATTALVSGETQMLIGSPAAVASQLAAGRLRLL
                     AVTSDARLTAFPKTPTVAEAGVPGYEYTGWVGVFAPKGTSPAIVDRMNGELKKAIESP
                     DTQKRMAELEPYIMTPQQMSARIKTDYEKYGRLLKLVGGKID"
     gene            1835..>2023
                     /locus_tag="VFL49_07615"
     CDS             1835..>2023
                     /locus_tag="VFL49_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET7911882.1"
                     /translation="MLDWTEYVKILVALLVIVDPVGAVPIFASLTLHNTTEEKHRIAR
                     VASLSVAIVLIVSAAGGQA"
BASE COUNT          354 a          660 c          612 g          397 t
ORIGIN      
        1 gacttgttct ggcgctcggt ggaaagcttc ttcaccgcgg cgaagtccgc cttcgccagc
       61 gcgcgggcct tgtcgcgcag caactgcgct tgcggactct tgcgcgccgc ttcgcccttc
      121 aggtcggccg taaccgccgg attgtggaac accggcgagc tgaagttgat cgcgaactcc
      181 aggctcggca tatccgatga gccggggcga gagtccttgt acgcaatctc gccgatcacg
      241 cgggtgctgc cgacatcgag cttcctgaag gcgtcctgcg tgccgctgac cgtcaggttc
      301 acgagcgaca tccgcggatc ctttggcgcg gcgagccagg tcaccagcac ggctttgcgg
      361 tcggcgggat cgaaccggag caggatgccc tgaaccttgt tctcccgcgc catctgcatg
      421 acgggcggaa acgccaggcc ggcgagcgcc tccgccggta cttcgcgatc ggcgatcaga
      481 atgcgcatct ccttcgggcg gtcgagcaat ccttccgcat tgtcgagctg gtaagccacc
      541 gagtacttga gcggcacatc cttaccgtcc gccttgagcg tgcccttggc cgtgcccgcg
      601 tcgatcgcga aggctgccga tgcaataggt atcaggaaaa ttcccatcgc gcgagctaac
      661 cgatttttca tattgcctcc catggctggc tttcagttgt cggcctggct ctgcagcctt
      721 ccgccttccg ccttcagtct atcttgcctc ccaccagctt cagcagcctt ccgtactttt
      781 cgtaatcggt cttgatgcgc gccgacatct gctgcggcgt catgatgtac ggctcgagct
      841 cggccatgcg cttctgcgtg tcggggctct cgatcgcctt cttcagctcc ccgttcatgc
      901 gatccacgat cgcgggcgac gtccccttcg gcgcgaacac gccgacccag ccggtgtatt
      961 cgtatccggg aacgccggct tccgcgactg tcggcgtttt cggaaatgcg gtgagccgcg
     1021 cgtccgaagt gacggcgaga agccgcaggc gaccggcagc gagctggctc gcaaccgccg
     1081 caggactgcc gatcagcatc tgcgtctcgc ccgataccag cgccgtcgtc gccgggccgc
     1141 cgcccttgta aggcacgtgc acgagcttcg tattcgtcat ggaagccaac aacacgaacg
     1201 cgaagtgcga gaagctgccg ctgcccgagg tcgcatagag aatctggtcg ggacgcgcct
     1261 tcgccagcgc gaccagctcc ttgaccgact tcaccggcag cgacggatgg acgaccagca
     1321 gcccggtctg cctcaccagc gtgccgatgc cgatgaaatc gccgagcgtg tcatacggca
     1381 gcttggggta gatgtacgga ttgccgaggt gcgccatgga atggaccatc agcgtgtagc
     1441 catcgggcgg cgactttgcg acgaggtccg cgccgatcga gcccgttgca cctggacggt
     1501 tatcgaccag gaactgctgg ccgagatttt cagacacttt ctgaaagaca atccgccccg
     1561 cgacgtcggt caggccgccg ctgggccagg gcgtgatgac gcgcaccggt tttcccggat
     1621 aaggctgcgc gaagctcgac gcggagagca aggccaggac agcgaacggg atcatcctgc
     1681 tgcgaatcat gaagaacctt tatgctgtct gagcgacgaa atcttacccc ctgcgccaac
     1741 ggccgcgctg cgcggagctc atccctcggg aaatctattt aaatatatga atatattata
     1801 ctttctcgca gccctgacag cgtagtgccg gcgcatgctc gactggaccg aatacgtcaa
     1861 gatcctcgtc gctttgctgg tgatcgtgga tcctgtcggc gccgtgccga tattcgcctc
     1921 cctgacgctg cacaacacga cagaggaaaa acaccgcatc gcccgcgtcg cgagcttgtc
     1981 cgtcgcgatc gtgctgatcg tctcggcggc aggcggtcag gcg
//
LOCUS       DASSRL010000376        15163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_517341, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000376 DASSRL010000000
VERSION     DASSRL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517341"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFL49_07620"
     CDS             complement(<1..540)
                     /locus_tag="VFL49_07620"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HET7911883.1"
                     /translation="MNDPDNFLNRWSRRKQEAGERKDKDKDKDEADKAPADSPSESEA
                     GASVSAPDAPSTPKFDLESLPPIESINADTDITAFMQAGVPEALKRAALRRVWTADPA
                     IRDFVGLNENFWDAAGPDGIAGFGDLDPNFDVKRMLSELFGEVPRQDASSDADAGRVA
                     NSSALPVPNQSEAASIPPSD"
     gene            complement(537..1055)
                     /locus_tag="VFL49_07625"
     CDS             complement(537..1055)
                     /locus_tag="VFL49_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HET7911884.1"
                     /translation="MSTIAPLASIEVGVVVERSKGVSQWVEFHWRPVSVLVGVPDTPP
                     WTKLTDEGDRATFYAGAAAVELYRTETAHYRDNLQMDPPQLWVVLRPVERDPPYELAI
                     VTADPAEGEGMTEAGSNIVESLPMPEPLREAVAAFVAEHHVERTFVKRKRDRANPDAL
                     ARHGPSTREDRK"
     gene            complement(1052..2059)
                     /locus_tag="VFL49_07630"
     CDS             complement(1052..2059)
                     /locus_tag="VFL49_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HET7911885.1"
                     /translation="MNDFWISSGHHLLDRGSDGGLHVTDEFLKLYLARPELIPPPEAC
                     AAERTLHATLLADPRTPVSAPDVAAIADSDARENWQLMIGFRDFLLRHKTLEAAFRDL
                     ARNGFGAVPPLFINQLVHVILRNALDGTEDARVARAAELFFRTQRVTLHDGSLIVADE
                     ETIGGVNTAPVTPLVSMLGIPPEADIDVINSENADSYWERSEQFDMALDLTAGREGLD
                     ALAKVMQRWIAHLLGIDVSIEALQELRNVDLGWYVGLDADATKIGDMLWRGEDVDETT
                     MARVVGLFRLTFADPVVMTEKIRGEPVYMFLAMTPDKTIRMKPQNLLTGLPVQHLETV
                     G"
     gene            complement(2056..2595)
                     /locus_tag="VFL49_07635"
     CDS             complement(2056..2595)
                     /locus_tag="VFL49_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HET7911886.1"
                     /translation="MKLLRTIRLDPSDTFVFEQAAEPGEWAVSGAFVFWNSDPSTLEG
                     KTRTAFRSGFLGVSTLGWSTLVQIVDATENDKVILAGKLAEQLMEKFGAPTKDEAVAA
                     AEEEIAFAASLCNQPSDTLIAVHRIFEDGAVKESFRTLRPRSGAKPARAFSFLEVEGE
                     DDQPAEDVNLVTMAGRDRK"
     gene            complement(2592..3350)
                     /locus_tag="VFL49_07640"
     CDS             complement(2592..3350)
                     /locus_tag="VFL49_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HET7911887.1"
                     /translation="MPLETARPQTRLAPELALPPPFAAIRLREVGDAFAHAVAVAPEK
                     GAGTLVYVGRFDLAEFAVVLEPDEPLLKARRVFYAGMAALADALAAHAQPETSIVIDW
                     PGSIRINGGLVGGGRLAWPEHTREVDTPKWLVFAGMIRTVSMTGTEPGLNPLVTALEE
                     EGFTDLMSSHVVESFARHFMVAIDAWQESGFAVVAKSYIERLPREQGIRRDIDDNGDL
                     LLRRMGKADVTRKKFLPRLAEPDWFDPQARGPRT"
     gene            3553..5529
                     /locus_tag="VFL49_07645"
     CDS             3553..5529
                     /locus_tag="VFL49_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7911888.1"
                     /translation="MPLDSAKVQRACRDSVVIEGRQFCRTELERVRKAAAGGEPVLVA
                     CTQEAPLFSEVAEDAGADTPTFVNIRETAGWSKDAAKAAPKMAALIAAGSAPAPDVSF
                     VTLASNGVALLYGRDERVIEAAELLKEHLDVTVLIKAGSELTPRRVTEFPVVEGIIRA
                     AKGYLGAFEVTVDDFAAPAPSSREVFAFGAARNGAVSRCDILFDLSGGPPLFPSADLR
                     DGYLRADPGDPAAVLRAVLKARDLTGTFDKPRYINFTEDLCAHSRSKIVGCRRCLDLC
                     PAGAIAPAGDHVTIDAHICAGCGQCAAACPTGAAAYALPPSDALLGKLRTLLAAYREA
                     GGTQPIILFHDAEHGGALIDMLARHHDGLPANVLPVEVNEVTPIGLESVAGAVAYGAV
                     AVRTLVRARPRHDIAGLHKTIALAEPILAGVGFNGARVATIETDDPEALGNALRAIPS
                     LAATAKPASFVATGRKREVMRHALRELHAAAPAPVNIVPLPAGAPFGTVEVNVEGCTL
                     CLSCVSACPTGALSDDPERPTLRFAEDACVQCGLCKATCPEKVISLRPQIDFRAATAM
                     SRIIKQEEPAECIRCGKAFGVKSSVERVVAKLEGKHWMYKDSKKRLDVIRMCDNCRVE
                     VMAEEQFDPFGAPRRPPARTTEDYFREREEKGES"
     gene            complement(5530..6831)
                     /locus_tag="VFL49_07650"
     CDS             complement(5530..6831)
                     /locus_tag="VFL49_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7911889.1"
                     /translation="MNFWTKNLGPVCLGLLLCPLAFSGAQAQLRGHGGPVKALAISPD
                     GTHAISGSFDTSAIRWSLSRNTAEQVLRFHEGAVNAVAYLKDGRIVTAGADAHVAIWS
                     AGQPQPDKILDGHSGPISSLAVSPDGSLLATASWDRSVRVWPLNGGVPRVLEGHGQNV
                     NGVAFTPDGKNLVSAGYDATLRIWPLAGGSAAVHTLPTPLNAVVVGADGEIATAGANG
                     KVYFLSSDGETLGETEASPNPVIALAISPDGNLVAAAGIRGSVAVIDRKTRKLAQTLV
                     GPGLPVWSVTFFPDSRTLLTGGADRMIRRWDASNGEPIGAVALGTPEDPLAAYAGDHG
                     AEVFRACVACHTLSPDEGNKAGPTLSGIFGRRIATLPGYNFSPALKKLDIVWTPETVS
                     RLFEIGPAHYTPGTKMPEQTIGSAEDRKALVDFLAKATTKK"
     gene            complement(6936..8924)
                     /locus_tag="VFL49_07655"
     CDS             complement(6936..8924)
                     /locus_tag="VFL49_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HET7911890.1"
                     /translation="MSETRKPRRSGDSDLIARVRAAARQEQFLEVVSAEEARARFEKH
                     LELKPLAAETVMLAGSRGRVLVQDVVAAVDAPPFDRSNVDGFAVRAADTVGAGDGNPK
                     VLPLNPEVITCGHTSVETVAAGTCTAIATGGVIPRGADAVVMIEQTELIEEGKPRIEL
                     RRAALPGQFISYAGSDIARGETLLRRGTCIDAREIGMLAACGLDRVDVVRRPRVAVLS
                     TGDELVEPGKPLPSAGVYDSNGAIIAASIAEAGGEPLVFGAFRDDAVALEKAVREAMD
                     ASDMVVLSGGTSKGAGDLSHRVVSRLGKPGILVHGVALKPGKPLCLGVIGNKPIVVLP
                     GFPTSAIFTFHAFVAPVIRTWAGLPPETEKTIAAHVPVRIVSELGRKEFVLVSLIESE
                     DATFAFPIGKGSGAVTSFSQADGFVEIDALSTVLNANSEARVTLLDKGKRTRDLVIMG
                     SHDVALDIVVGALANRGFVARTLAVGSQGGVAAINRGECDIAPVHLIDPVTGHYNKHL
                     VEPGLTLVPGWRRMQGVVFRSGDRRFEGRSAQEALKIVLADASALMVNRNVGAGTRVL
                     MDRLLDGARPAGYANQPKSHNAVAAAIAQGRADWGLAIEPVAKLYGLGFLPVAPEHYD
                     FLVADARRSHPAVQTFIDVLRDQETRERIRALGMQPTD"
     gene            complement(8935..10056)
                     /locus_tag="VFL49_07660"
     CDS             complement(8935..10056)
                     /locus_tag="VFL49_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET7911891.1"
                     /translation="MAVSEAIQTISRLTPLADVLLLIDAEVKPVTPRTIDVSAAAGRA
                     LATDAMAPARPTASLALQDGWALNSDETLGAGGYAPAPLARMPSRVEAGQAMPAGADS
                     IAPIDAVKVGGGVAEVLMPINPGEGVLAAGGDSDPAIPLRRASERLTATDLAAFAAAG
                     IARVTVREPRFRVLPLRGSGIITAAAQLICTDIERRGGAARFDDSGRGLGVAMAAESA
                     DAIIAIGGTGSGRNDNAVQILSREGRLAVHGVALTPGETAAIGFVGTTPVLLLPGRLD
                     AALAVWLVVGRRILDRLAAAKNNDGDVAESLPLARKVASTVGLTELVPMRRNAGHAEP
                     LASKYLPLSSLARSDGWILVPADSEGYSAGSPVQLRPWP"
     gene            10115..11335
                     /locus_tag="VFL49_07665"
     CDS             10115..11335
                     /locus_tag="VFL49_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET7911892.1"
                     /translation="MQRGFLLVKSGEVSVFTATKGKPLLTTTTGALPRPSWYTENLRG
                     IPLSQGFSRLAYREQHFDCLACHVAAQHRAGIDIFVDGDARLDDDVAGRAWVSYVTER
                     IAGIGVPRVEVPPASFMADKGPGDLMWEVIETRMTPPVTGKIGETTLQLDRAYKAIAP
                     MTERPVKIGSISAQILALMLTNEHYKNRLELLMDLSIALNKEYHALADAGAPLLQVEE
                     PAIHQIIADPGQPIKPEKWVEAFNTEIKGLRSKCEVWCHTCWGSPAAQRVASKDQSYK
                     DALPYLDQLDVDVLTIEGAFNRGLDFEHVGEMISKDKKIAVGVISHRTLQVERPEEVA
                     DLIRKALKYIEPERLILTSDCGFGRQSMSRMHAFYKMVSLTRGTNIVRNELGLEEAYI
                     PATDKQLSMVLLGA"
     gene            11332..11556
                     /locus_tag="VFL49_07670"
     CDS             11332..11556
                     /locus_tag="VFL49_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7911893.1"
                     /translation="MTETLMNLRGLKCPLPALRVRKKLSGLKTGDLIIAECTDPLTTI
                     DIPNLLRQTGDTLEDRSEADGVLTFRIRKG"
     gene            complement(11561..12400)
                     /locus_tag="VFL49_07675"
     CDS             complement(11561..12400)
                     /locus_tag="VFL49_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7911894.1"
                     /translation="MSDPRSLIRAALMERFGQEVRVGNDLTGLDEIARIAAHRVHRRY
                     LARDVAPDVLRLLCACALSAPSKSDLQQGDILVIRDRTKRDAIAGLLPEMPWLNEAPV
                     FLVFLANGRRLPEVSRLRGRPFPNDHLDLFFNATVDAAIVMTTFLRAAEAVGLGCCPI
                     SVIRDHAVVISEMLQLPAKVIPVAGMCVGWPAEEGGITPRLPLDVTIHEERFMESDLA
                     GKIDAYDRRRAATRPYRGQRDTERFGRSEFYGWSEDKARQYAVPLRVDFGAFVRAKNF
                     NLD"
     gene            complement(12590..12856)
                     /locus_tag="VFL49_07680"
     CDS             complement(12590..12856)
                     /locus_tag="VFL49_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET7911895.1"
                     /translation="MRKIFLLAAMALTLAGCQTAREDRVLGGSLIGGGTGALIGGLAG
                     GSAGAAVAGGLVGAAAGAIIADATRPGRCYYYTRHGYRRYVRCP"
     gene            13074..14342
                     /locus_tag="VFL49_07685"
     CDS             13074..14342
                     /locus_tag="VFL49_07685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7911896.1"
                     /translation="MADKDIETVIVGGGAAGIAAARRLHGAGVKCLIVEARPRLGGRA
                     WTVADPAGFLIDLGCGWLHSADRNPWVDVAAEQGKTIDKTPPPWRRPSIPTGFPIGGQ
                     KEFLKSQEEFFTRVGERAQKDPDVAAAELLTPGGRWNNLINAVATYISGAELDRLSAR
                     DFDNFDDTGVNWRIVEGYGATIAACGHALPAALDCPVRTIDHSGKRLKIVTPHGTIET
                     ENVIVTVPTSILAESSEFFTPALPDKVTAAQNLPLGLADKLFLSLEHAEEFEKDSRLF
                     GATDRTATATYHLRPFGRPQIEVYFGGRCAADLEANGENAFFDFAVSQLVGLLGSKFA
                     QRVKPIRIHRWRADPFARGSYSYAVPGMTDCRGVLAAPVDGRIFFAGEACSRADFSTA
                     HGGWKTGVAAAEQVLRARNHAHDHIKSKSK"
     gene            14444..>15163
                     /locus_tag="VFL49_07690"
     CDS             14444..>15163
                     /locus_tag="VFL49_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7911897.1"
                     /translation="MRVRFWGVRGSIACPGPSTIRYGGNTPCIEVRCGEHVLVFDAGT
                     GLRPLGLELVKDKSIRHVDIFITHCHLDHVVGLPFFAPLFRKGYRVRVWAGNLLPSNS
                     IERVMRMLMSSPLFPIQIEIFKAGIEFHDFRSGDVLRPHENITLHTAPLDHPDGSNGY
                     RLEYGGRTFSLVSDTEGFPGKCDNQLLSLADRADLMVYDATFTEDEIASRIGWGHSTW
                     LRGIRLADQARVKHLCLFHHDP"
BASE COUNT         2978 a         4846 c         4528 g         2811 t
ORIGIN      
        1 gtccgatgga gggatgctcg ccgcttcgga ttggtttggg actggcaaag ctgaggaatt
       61 cgccacgcgg cccgcgtctg catcagacga agcgtcctgg cgcggcacct ccccgaagag
      121 ctcagagagc attcgcttga cgtcgaaatt gggatcgagg tccccaaagc ccgcaatccc
      181 atcgggtccg gctgcgtccc agaaattctc gttcagtccc acaaagtccc ggatggccgg
      241 gtcagccgtc cagacgcgac gaagcgccgc acgcttcagc gcctcgggca caccggcctg
      301 catgaaagcc gtgatgtcag tgtccgcatt gatcgattcg atcggtggca ggctttcgag
      361 atcgaatttc ggagtcgacg gtgcgtcggg agcagagacc gatgcacccg cttcgctttc
      421 agacggagaa tctgcagggg ctttgtctgc ttcatccttg tctttgtctt tgtctttgcg
      481 ttcacccgcc tcctgtttgc ggcgcgacca acggttaaga aaattgtcag gatcgttcat
      541 ttgcgatcct cccgtgttga gggaccatgc cgcgccagcg cgtcgggatt tgcgcgatcg
      601 cgctttcgct tcacaaatgt acgctcgaca tggtgctccg ctacgaaagc cgcaacggct
      661 tcccgcagcg gttccggcat cggcaacgat tcgacgatat tcgaacccgc ctcggtcatc
      721 ccctcgcctt cggccggatc ggcggtgaca atggcaagct cgtacggagg gtcgcgctcg
      781 accggacgta agacgaccca caattgcggc ggatccatct gcagattgtc tcgatagtgc
      841 gcggtctccg tccggtacaa ttccacagcc gctgcgccgg cataaaaagt tgcgcggtcg
      901 ccctcatcgg tcagcttcgt ccatggcggt gtgtccggca cccctaccag cacgctgacc
      961 ggacgccagt gaaactcgac ccattgactc acacccttgc tgcgttcgac gacgacgccg
     1021 acttcgatgc tcgcaagcgg cgcaatggtg ctcaaccgac agtctccagg tgttgtaccg
     1081 gcaaaccggt caatagattt tgcggcttca tgcggatggt cttgtccggc gtcattgcca
     1141 aaaacatgta gacgggttca cccctgattt tttcggtcat caccaccgga tcggcgaatg
     1201 taagccgaaa caggccaacg acgcgtgcca tcgtcgtctc gtcaacatcc tcgccgcgcc
     1261 aaagcatatc accgatcttc gtcgcgtcgg catcgaggcc gacataccag ccaagatcga
     1321 cattccgcag ctcttgcaac gcttcaatgc tgacatcgat gccgagcaaa tgtgcgatcc
     1381 agcgctgcat cactttcgcc aaagcatcaa gcccttcgcg gccggcggtc aaatcgagcg
     1441 ccatgtcaaa ctgctcgctc cgctcccaat aactgtctgc gttttcgcta ttgataacgt
     1501 cgatatcggc ctcgggcgga atccccagca tggaaaccag cggtgtgacc ggcgcggtgt
     1561 tgacgccacc gatagtttcc tcgtcggcga cgatcagcga tccatcgtgc agcgtgacac
     1621 gttgggtgcg gaaaaagagt tcggcagcgc gcgccactcg cgcatcctcg gttccgtcca
     1681 gggcattacg caaaatcacg tggacgagct ggttgatgaa aagcggtgga acggccccaa
     1741 agccgttgcg cgccaggtcc ctgaatgctg cttcgagtgt cttgtgccgc aacaggaagt
     1801 cgcgaaaccc tatcatcaac tgccagtttt cccgcgcatc ggaatcggca atcgccgcaa
     1861 cgtccggcgc tgaaactggt gtgcgcgggt ccgcaagcag cgtagcgtgc agcgtgcgct
     1921 ccgccgcgca ggcctccggc ggcggaatca attccgggcg cgcgagatac agcttgagaa
     1981 attcatcggt aacgtgcagc ccgccgtccg aaccacggtc gagcaaatga tgcccgctgg
     2041 agatccagaa gtcgttcatt tgcgatcccg acctgccatc gtaacaaggt tgacgtcttc
     2101 cgcaggctga tcgtcctcgc cttcgacctc aaggaacgag aaggcacggg caggctttgc
     2161 tccgctgcgc ggacgcaacg tgcgaaacga ttctttcacg gcgccgtctt caaatatgcg
     2221 gtgcaccgcg atcagcgtat cggagggctg attgcagaga gatgcagcga aagcaatctc
     2281 ctcttcagcc gccgcgaccg cctcgtcctt ggtaggcgcg ccgaattttt ccatcagctg
     2341 ttcggccagc ttgccggcca ggatcacctt gtcgttctcg gtcgcatcca cgatctgcac
     2401 gagcgtcgac catccgagcg tggacacgcc gagaaagccg ctgcgaaagg cagtacgcgt
     2461 cttgccctcg agcgtcgatg gatcgctgtt ccagaacacg aatgcgccgg ataccgccca
     2521 ctcaccgggc tcggcggcct gctcgaacac aaaagtatcg gacgggtcga gccttatggt
     2581 gcgcaggagc ttcacgtgcg cggacctctt gcctgaggat cgaaccagtc gggctcggcg
     2641 agtcgcggca gaaacttctt tcgcgtgacg tccgcttttc ccatgcggcg gagcaggagg
     2701 tcgccgttgt catctatgtc gcgtcgtatg ccctgctcgc gcggcagccg ctcgatatag
     2761 ctttttgcga cgactgcaaa tccactttcc tgccacgcat cgatcgcgac catgaaatgc
     2821 cgcgcgaagc tttcaacgac gtgactcgac atcaagtcgg tgaaaccttc ctcttccagc
     2881 gcagtgacaa gaggattcaa tccaggctcg gtaccggtca tcgaaacggt tctgatcatg
     2941 ccggcgaaaa cgagccactt cggcgtatcg acttcacgcg tgtgctccgg ccaggcaagc
     3001 cggccgccgc cgacaagccc accgttgata cgaattgaac ccggccagtc gatcacgatt
     3061 gacgtttccg gctgggcatg ggcggcgagc gcgtcggcga gtgccgccat accggcgtag
     3121 aagacgcggc gcgctttcag cagcggctcg tcgggctcga gcacgacggc gaattccgcg
     3181 aggtcgaagc gcccgacata gaccagcgtg ccggcacctt tttcaggcgc aaccgccacc
     3241 gcgtgggcaa aggcgtcgcc gacctcgcga agacggatgg ctgcaaatgg cggcggcaat
     3301 gccagttctg gcgcgaggcg cgtttgcggg cgggccgtct ccagcggcat tgtacctcac
     3361 tctaccattc actttagaat gcatataatt atagcccgga tggtcccgag gccagacccg
     3421 cgggtcgtcg gggacagcgc caggcagcgc agttcgtgcg tctgacctgg atcggcggga
     3481 caagcgaaaa ggttaacgaa ccagatggcc gaacggccgc gtaacatcct gatctgctcg
     3541 tgcgaaggca ccatgcccct cgacagcgca aaggtgcagc gcgcatgccg cgattccgtg
     3601 gtgatcgagg gccggcaatt ttgccgcacg gaactagagc gggtccgcaa ggcggcggcc
     3661 ggcggcgagc cggttttggt cgcatgcacg caggaagcac cgctgttcag cgaagtcgcc
     3721 gaagacgccg gcgctgatac tcctaccttc gtcaacatcc gggaaacagc gggctggtcc
     3781 aaggacgccg ccaaggctgc gcccaaaatg gcggcgctga ttgcggcagg cagtgcgccc
     3841 gcgccggacg tgtcgttcgt cacgctggcg agcaatggtg tcgcgctgct ttacggcagg
     3901 gacgagcgtg tcatcgaggc ggccgaacta ctcaaggagc accttgacgt aaccgtgctg
     3961 atcaaagccg gttctgaact tacgccgcgg cgggtgaccg aatttccggt ggtggagggc
     4021 atcatccgcg ctgcgaaggg ctatctcggt gccttcgagg tcacggttga tgattttgcg
     4081 gcgcctgccc cgtcctcgcg cgaggttttt gcatttggag ccgcgcgcaa cggcgcggtg
     4141 tcacgatgcg acattctttt cgatctttcc ggcgggccgc cattattccc ttccgccgat
     4201 ctgcgcgacg gctatctgcg cgcggatccc ggcgatccgg ccgccgttct tcgcgccgta
     4261 ctcaaggctc gcgacctgac cggcacgttc gataaaccgc gctacatcaa ttttactgaa
     4321 gacctctgcg cgcattcgcg atcgaagatc gtcggctgcc ggcgctgtct cgatctttgt
     4381 cccgccggtg cgattgcgcc tgccggcgat catgttacga tcgacgctca tatctgtgcc
     4441 ggatgcggac aatgcgcggc cgcctgcccg acgggcgcgg ctgcctacgc gctgccgccc
     4501 tccgacgccc tgttggggaa attgcggacg ctgctcgctg cctatcgtga ggcagggggc
     4561 acccagccca tcatcctgtt tcatgatgcc gagcacggag gcgccttgat cgacatgctt
     4621 gcacggcacc acgatggatt gccggcgaac gtgctgcccg tcgaagtcaa cgaggtcacg
     4681 ccaattgggc tggagtctgt tgccggagcg gtcgcctatg gcgcagtcgc agtacgcacg
     4741 cttgttcgcg ccaggccgcg ccacgatatc gccggactgc acaagacgat cgcgctggcc
     4801 gaaccgattc ttgccggtgt cggattcaac ggcgcgcgcg ttgccacaat tgaaacagac
     4861 gaccccgaag cgctgggcaa tgcattgcgc gcaatacctt ctcttgctgc gacggcaaag
     4921 cccgcgtcat ttgtggcaac cggccgcaag cgtgaagtga tgcggcatgc gctccgcgag
     4981 ctgcatgcag cggcgccggc tccggtcaat atcgtgccgc tgccggcagg cgcgcccttc
     5041 gggaccgtcg aggtcaatgt cgaaggctgc acgctgtgtc tttcctgcgt ctcggcctgc
     5101 ccgacaggtg ccctttcgga cgatcctgaa cggccgacat tgcgcttcgc cgaggacgcc
     5161 tgcgtgcagt gcggactgtg caaggcgaca tgcccagaaa aagtgatcag cctgcgaccg
     5221 caaatcgatt tccgcgcggc caccgcaatg agtcgcatta tcaagcagga ggagcccgcc
     5281 gagtgtatcc gctgcggcaa ggctttcggc gtgaagagct cggtcgagcg agtcgttgcc
     5341 aagctcgaag gcaagcattg gatgtataaa gactcgaaga agcgactcga tgtcatccgc
     5401 atgtgcgaca actgccgcgt cgaggtgatg gcggaagaac aattcgatcc cttcggcgca
     5461 ccgcggcggc cgccggcgcg taccaccgag gattatttcc gcgaacgcga ggaaaagggc
     5521 gaaagctgac tactttttcg tcgtggcctt tgccagaaag tcgaccagcg ccttgcggtc
     5581 ttcagccgat ccgatcgtct gctccggcat cttggtgcct ggcgtatagt gcgccggtcc
     5641 gatctcgaac agcctggata cggtttccgg cgtccacaca atgtcgagct tcttcagtgc
     5701 cggcgagaaa ttgtagccgg gcagcgtggc aatgcggcgt ccgaatattc ccgaaagtgt
     5761 cggtccggcc ttgttgcctt catcaggaga caatgtgtgg cacgcaacgc aggcccggaa
     5821 aacctcggcg ccgtggtcgc cggcataggc cgccagggga tcctccgggg tgccgagcgc
     5881 cacggcaccg atcggctcgc cgttcgatgc atcccaacgg cggatcatgc ggtcggcacc
     5941 accagtcagc agagtccggc tgtccgggaa aaaggtcacc gaccataccg gcagacctgg
     6001 tccgacaagc gtctgcgcaa gcttgcgcgt ctttcgatca atgacagcaa cggatccgcg
     6061 aatgcccgcg gcggcaacga gattgccgtc gggagaaatg gcaagggcga tgacaggatt
     6121 gggcgaagcc tcagtctcgc ccagcgtctc accgtcagac gacaggaagt agactttgcc
     6181 attcgctcca gccgtcgcaa tctcgccgtc cgccccgacg acgacggcgt tgagcggcgt
     6241 cggcaatgtg tgaaccgccg cgctgccgcc cgcgagcggc cagatgcgca gcgtcgcatc
     6301 gtagcccgcg ctcaccagat tttttccgtc cggcgtaaat gcaacgccgt tgacgttctg
     6361 cccgtggccc tcaagcacac ggggaactcc tccgtttaat ggccagaccc ggacgctgcg
     6421 gtcccacgat gccgtggcca acaggcttcc gtccggagag acggcaaggc ttgagattgg
     6481 cccggaatgc ccgtcgagaa ttttgtcagg ctgcggctgc ccggcagacc aaatggcgac
     6541 atgcgcatcg gcgccggccg ttacaatgcg tccatccttg agataagcga ccgcattcac
     6601 cgcgccttcg tggaagcgaa gaacctgctc ggcagtattg cgggacagcg accagcggat
     6661 cgcggatgta tcgaaactgc ccgagatcgc gtgcgttccg tcaggcgaaa ttgccagcgc
     6721 cttcaccggc ccgccgtgac cgcgcaattg agcctgcgct ccgctgaagg cgagcggaca
     6781 caaaagcagg ccaagacaga ctggcccgag attcttcgtc caaaaattca tggtccgccc
     6841 taaactatcg acccgaggtt agagcatttt gaggcatccc ggtcctgttt ccgcctcact
     6901 ttcggccctc gcgcagtctt tttgtccaac ggtgttcaat cggtcggctg catgccgaga
     6961 gcgcgaatgc gctcgcgtgt ctcctggtca cgcaacacgt cgataaaggt ctgaaccgcg
     7021 ggatggctcc gccgcgcatc ggccaccagg aagtcgtagt gctccggcgc caccggaaga
     7081 aatcccaaac cataaagctt cgcgacaggc tcgatcgcaa gaccccagtc ggcgcgccct
     7141 tgagcgatcg ccgctgccac cgcgttgtgg gacttcggct ggttggcgta accggccggt
     7201 cgtgcgccgt ccaacaggcg gtccatcaaa acacgcgtgc cggcacccac gttccgattg
     7261 accatcaaag cggaggcatc ggcgagaaca atcttcagcg cttcctgcgc actgcggcct
     7321 tcaaagcgcc ggtcgccgga ccggaacacg acgccttgca tccggcgcca tccgggaacc
     7381 agagtcaggc ccggctccac cagatgctta ttataatgtc cggtcacggg atcaatcaga
     7441 tggacgggag caatgtcgca ctcaccgcga ttgatcgctg caactcctcc ttgactgccg
     7501 accgccagag tgcgggcaac aaagccacgg ttcgcgaggg cgccgacgac gatatcaagc
     7561 gcgacgtcat gactgcccat gattacgaga tcgcgcgtcc gcttgccctt gtcgagcagg
     7621 gtcacccgcg cctcgctgtt ggcgttgagc accgtggaga gcgcatcgat ttcgacaaag
     7681 ccatcggcct gcgagaaact ggtcactgcg cctgagccct tccctatcgg gaaggcaaac
     7741 gtcgcgtctt cgctctcaat aagtgaaacc agcacgaatt ccttgcgccc aagctcggag
     7801 acgatccgta ccggcacgtg tgccgcgatg gttttctcag tttccggcgg cagcccggcc
     7861 catgtgcgga tcacgggagc aacgaaggca tgaaatgtga aaatcgccga agtcgggaag
     7921 ccaggcagaa cgacgatcgg cttgttgccg atcacgccca ggcacagcgg cttgcccggt
     7981 ttgagcgcga cgccatgcac gagaatgccc ggcttgccga ggcgtgacac cacacgatgc
     8041 gacaggtctc ctgcgccttt cgatgtacct cctgacaaca ccaccatgtc gcttgcatcc
     8101 atcgcctcgc gcacggcttt ttccagagcc accgcatcgt cacgaaaggc gccgaacacg
     8161 agcggctcgc cgcccgcttc tgcgatcgac gcagcaatga tcgcgccgtt gctgtcgtat
     8221 acgccagcgg atgggagcgg cttgcccggc tcgacgagtt cgtcgccggt cgacagcaca
     8281 gccaccctgg gacgccggac cacatcgacg cgatcgagtc cacaggccgc cagcatgccg
     8341 atttctcgcg cgtcgatgca tgtgccgcgc ctgagcaagg tttcgccgcg cgcgatatcc
     8401 gagccggcat aggaaatgaa ctgtccaggc agcgcagcac gccgcagctc aatgcggggc
     8461 ttgccttcct caatcagttc ggtctgttcg atcatgacca cggcgtcggc gccgcgcggg
     8521 atgacgcccc ccgtggcaat cgccgtgcat gttcccgccg ccaccgtttc aacggatgtg
     8581 tggccgcacg taatcacttc cggattgagt ggcaacacct tcggattgcc gtcgcccgcc
     8641 cccacggtat ccgccgcacg cacggcaaag ccgtcgacat tcgagcggtc gaacggcgga
     8701 gcatcgactg cggcaaccac atcctgcacg agcactcggc cgcgcgagcc ggcaagcatg
     8761 acggtctcgg ctgcaagcgg cttgagctcg aggtgctttt cgaagcgcgc acgtgcttcc
     8821 tccgccgaca ccacttcgag gaactgctcc tggcgggcgg cggcgcgcac acgcgcgatc
     8881 agatcgctgt cgccgctacg ccgcggcttg cgcgtttcgc tcatgatgac cggctcatgg
     8941 ccacggcctt agctggactg gcgagccggc agaatatcct tcgctatcgg cgggcaccaa
     9001 tatccagccg tcggatcgcg ccagcgacga gagcggaaga tatttgctcg ccaacggctc
     9061 cgcgtgaccc gcattgcgcc gcatcggaac cagctcggtc aaaccgacgg tcgaagcgac
     9121 cttgcgcgcc agcggcaagg attccgccac gtcaccatcg ttgttcttcg ccgccgccaa
     9181 tcggtcaaga atccgccggc ccaccacaag ccacaccgcc agcgcggcat caagccgtcc
     9241 gggcagcagg aggacaggcg ttgtgcctac gaatccgatg gcggcggtct cgcccggggt
     9301 gagtgcaacg ccgtgcaccg caagccggcc ttcgcgcgaa agaatctgca cggcgttgtc
     9361 gttgcggcca ctcccggtac caccgatagc gataatcgcg tcggcgctct ccgccgccat
     9421 cgccacgccg agaccacgtc cgctgtcatc gaaacgcgcg gcaccaccgc gccgttcgat
     9481 atccgtgcag atcaattgcg ccgcggctgt gatgatgccg ctgccacgca atggcagaac
     9541 gcgaaagcgc ggctcccgca ccgtgacccg tgcgataccg gcggctgcga atgctgcaag
     9601 atccgtagcg gtcaatcgtt cactggcgcg tcgcaatggg attgccggat cactgtcgcc
     9661 acccgccgcc aggactccct ctccggggtt gatcggcatc agcacctcgg caactccgcc
     9721 gccgaccttc accgcatcga tcggcgcgat gctgtccgcc cctgcgggca tcgcctgccc
     9781 ggcttcgacg cgtgagggca tgcgcgcaag cggagccggc gcgtacccgc ccgccccgag
     9841 cgtctcgtcg gagttcagcg cccagccgtc ctgcaaggcc agcgacgcgg tcgggcgcgc
     9901 cggcgccatg gcatcggtcg caagcgcgcg ccccgcagcc gcagagacat cgatcgtgcg
     9961 cggcgtgacc ggcttcacct ccgcatcgat cagcaacagc acatcggcga gcggcgtcag
    10021 ccgtgaaatc gtctgtattg cttcggacac cgccatttgc cgtgcttgac cttgttgcag
    10081 tcagagactt cagtttacct gaacaacacc gcgcgtgcag cgcggctttc tgctggtgaa
    10141 atcgggagaa gtaagcgtgt ttacggcgac aaaaggcaag cctcttctca ccaccacgac
    10201 cggagcgctg ccccgcccaa gctggtacac ggaaaacctg cgcggcattc ccctctccca
    10261 gggcttttcc cgtcttgcct atcgcgagca gcactttgat tgcctcgcat gccacgtggc
    10321 cgcccagcat cgtgccggca tcgacatctt cgtcgacggt gacgcgcgcc tcgacgacga
    10381 cgtcgccgga cgcgcctggg tgagttacgt cactgagcgc attgcgggca tcggcgtccc
    10441 gcgcgttgaa gtgccgcccg caagcttcat ggctgacaaa gggcccggcg atctgatgtg
    10501 ggaggtgatc gaaacgcgca tgacgccgcc agtcaccggc aagatcggcg aaacgacgtt
    10561 gcaactcgat cgtgcctaca aggcgatcgc gccgatgacc gaaaggccgg tgaagatcgg
    10621 ctccatctcg gcgcagatcc tggcgctgat gctcacgaac gagcactaca aaaaccgtct
    10681 cgaattgctg atggatctgt cgatcgctct caataaagag tatcacgcgc tcgccgacgc
    10741 aggtgcgccg ctcctccagg tcgaagagcc ggcaatccac cagataatcg ccgatccggg
    10801 acagccgatc aaaccggaga aatgggtgga ggcgttcaac accgagatca agggcctgcg
    10861 cagcaaatgc gaagtgtggt gccacacgtg ctggggcagc ccggcggcgc agcgggtggc
    10921 gagcaaggat caatcctata aagatgcact gccctacctc gaccaactcg acgtcgacgt
    10981 gctgacaatc gagggcgcgt tcaaccgcgg actcgatttc gagcatgtcg gcgagatgat
    11041 ctcgaaagac aagaagatcg cagtcggcgt catcagtcat cgcaccttgc aggtggagcg
    11101 tccggaggaa gttgccgatc tgatccgcaa ggcgctcaaa tatatcgaac cggaacggct
    11161 gatcctgacg agcgactgcg gcttcggacg gcaatcgatg agccgcatgc atgcttttta
    11221 caagatggta tcactcacgc gcgggaccaa catcgtgcgc aacgagttgg ggcttgagga
    11281 agcctatatt cctgcgaccg acaaacagct gtcgatggta ctcctcggcg catgactgaa
    11341 acgctgatga atctgcgcgg actcaaatgc ccgctgccag cattgcgggt gcgcaagaag
    11401 ttgtcagggc tgaagaccgg cgatctgatc atcgccgagt gcaccgaccc gctcaccacc
    11461 atcgacattc cgaacctgtt gcggcagacc ggcgacacgc tggaggacag atcggaggcc
    11521 gacggcgtcc tcacgttccg catccggaag ggctgatatc tcagtcaaga ttgaagttct
    11581 tggcgcgcac gaatgcgccg aagtcgacgc gcaacggcac cgcatattga cgtgccttgt
    11641 cttcggacca gccatagaat tcgctgcggc cgaaacgttc cgtgtcgcgt tgacctcgat
    11701 aagggcgcgt cgccgcgcgg cggcggtcat aggcgtcgat ctttccggcg agatcactct
    11761 ccatgaagcg ctcttcatgg atcgtgacgt cgagaggcag ccgcggcgtg atgccgccct
    11821 cctcggccgg ccagccgacg cacatgccag ccaccggaat gactttcgcc ggcagctgca
    11881 gcatttcgct tatgaccacg gcatgatcgc ggatcacgct gatggggcag cagccgagac
    11941 cgacggcttc ggccgcacgc aggaatgtcg tcatgacaat ggcggcgtcg accgtcgcgt
    12001 tgaagaaaag atcgagatga tcattgggaa acggcctgcc gcgcagccgc gaaacctcgg
    12061 gaaggcgtcg gccgtttgcg agaaacacca gaaacaccgg agcctcgttc agccatggca
    12121 tctccgggag caggcctgcg atcgcatcgc gcttcgtccg gtcgcggatg acaaggatat
    12181 cgccttgctg caagtcgctc ttcgacggcg ccgaaagcgc gcacgcgcaa agaagccgca
    12241 gcacgtcggg cgcaacgtcc cgcgccagat aacggcgatg cacacggtgc gctgcaatcc
    12301 gggcgatttc atcgaggccg gtcaaatcat tgccgaccct tacctcctgg ccgaaacgtt
    12361 ccatcagggc ggcccggatg agagacctcg gatccgacat ccacagctcc tttgtcgaat
    12421 actgttcgcg gcggcatcgt agcgcaggcg aacaggataa ggacacgccg gttgcggaac
    12481 tatcaatgaa cgaagcctgg cggcatgccg gcgcgcgctt gagcctgtca gcgccgccgg
    12541 cgctgtgctg aagcccactg tctggaaaat gtagcgatag gaccgcgccc tacgggcagc
    12601 gcacgtagcg ccggtatcca tgccgcgtgt aatagtaaca gcgccccgga cgagtcgcgt
    12661 cggcaattat cgcgccggca gcggcaccaa ccagcccacc ggcaaccgct gcgcctgccg
    12721 acccgccggc aaggcctccg atcagagcgc ccgtgccgcc accgatcagc gagccgccaa
    12781 gcacgcgatc ctcacgcgcc gtctggcatc cggcaagcgt cagcgccatc gcggcgagca
    12841 agaaaatctt tcgcattcca tccctccgtc ccgtttcttg gctgcaaaaa attccgtcga
    12901 tgtgggtcaa ttttgcagca gagcatcaac ccgcatgcat caattgggga aatgtgcgtg
    12961 cggtttgcga ttgatttgtg ccatgcaacg ggaggctttc ggaggtatca aaatcttcat
    13021 tccgtaccta catccggctg ttgtcccaag cgcccgaacg aagatttccg caaatggcgg
    13081 acaaagacat cgaaaccgta attgtcggcg gcggcgcggc cggaatcgcg gccgcccgac
    13141 ggcttcacgg tgccggtgta aaatgtctca tcgttgaggc gcgcccgcga ctcggcggcc
    13201 gtgcgtggac agttgcggac ccagccgggt ttcttatcga tctcggttgc ggttggcttc
    13261 actctgccga ccggaatccg tgggtggatg ttgccgcaga acaggggaag acgatcgaca
    13321 agacgccgcc accatggagg cgaccttcaa ttccgaccgg atttccgatc ggcggtcaaa
    13381 aggaattcct caaaagtcag gaagagtttt tcactcgggt tggcgaacgc gcgcaaaagg
    13441 accccgacgt cgcggcggcc gaactgctca caccgggcgg gcggtggaat aatttgatca
    13501 acgccgttgc tacctacatc agcggcgccg agcttgatcg tttgtcggca cgagacttcg
    13561 acaatttcga cgataccggc gtcaactggc gcatcgtcga aggttatggt gcgacaatcg
    13621 ctgcttgtgg acatgcgttg ccggccgcgc tcgattgtcc ggtgcgaaca attgatcaca
    13681 gcgggaagcg actcaaaatc gtgacgccgc atggcacgat tgagaccgaa aacgttatcg
    13741 tcacggttcc aacctcgatc ctcgccgaat ccagcgaatt ttttacgccc gccctgccgg
    13801 acaaagtgac tgccgcacag aatctgccgc tcggacttgc cgacaagctt tttctctcgc
    13861 tcgagcacgc cgaagaattc gagaaggaca gccgcctgtt cggcgcgacc gatcgaaccg
    13921 ccacggcgac ttaccatttg cggccattcg gccgcccgca gatcgaagtt tatttcggcg
    13981 gacgatgcgc agccgatctc gaggcgaatg gcgagaatgc cttcttcgat tttgcggtgt
    14041 cgcagcttgt cggtctactg ggcagcaaat tcgcgcaaag agtaaagcca attcgaatcc
    14101 atcgttggcg cgcagatccc ttcgcgcgcg ggtcttattc atatgcagtt ccgggaatga
    14161 ctgactgccg cggcgtgctt gcggcacctg tagatggccg gatttttttc gcaggcgagg
    14221 cctgctcccg cgcagacttc tctaccgcgc acggcggatg gaaaacgggc gtggccgccg
    14281 ccgaacaggt tttgcgcgca cgcaaccacg ctcatgacca cataaaatcg aaatcaaaat
    14341 aaatccggtc gatccgcccg cagccacaac tcgacattgt catccattcc aaatacaaac
    14401 atccgacgtg atttccgggc gtgcgggaat gtcaaaatca ccgatgcggg ttcgattttg
    14461 gggtgttcga ggaagcattg cctgtcccgg cccatcgaca attcgttacg gcggaaatac
    14521 cccttgcatc gaagttcgct gcggtgagca tgttcttgtt ttcgacgccg gcaccgggct
    14581 gcgcccgttg ggtctggagc ttgtcaaaga caaaagtatc cgtcacgtcg acattttcat
    14641 cacgcactgc catctcgatc atgttgtagg cttgccattc ttcgctccgt tatttcggaa
    14701 gggctatcgc gtccgtgtct gggccgggaa cctgctgcct tccaacagca tcgagcgcgt
    14761 gatgcggatg ctgatgagct cgccgctttt tccgatccag atagaaatct tcaaggctgg
    14821 cattgaattt cacgacttcc gttcaggtga cgtcctgcgt ccacatgaaa atataacctt
    14881 gcacaccgcc ccgctcgatc atccggatgg cagcaacgga tatcgcctcg aatacggcgg
    14941 ccgcactttt tcgctggtca gcgacacgga gggattcccc ggaaaatgcg acaaccagct
    15001 cttgtcgctg gctgatcgcg ccgacctgat ggtctacgat gcgacgttta ccgaggatga
    15061 aatcgcatca cgaatcggct ggggacactc gacatggctg cggggaattc gcctcgccga
    15121 tcaggcccgc gtaaagcatc tctgcctgtt ccaccacgac cca
//
LOCUS       DASSRL010000377         7616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_832243, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000377 DASSRL010000000
VERSION     DASSRL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832243"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFL49_07695"
     CDS             complement(<1..339)
                     /locus_tag="VFL49_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7911898.1"
                     /translation="MSEARLTSITVGSTVSGRSIAVRERTGSNPAIFWLGGFKSDMKG
                     TKAEALSAWARENGRACVRFDYSGHGESGGDFRAGTLGRWLDESLAVFSACCAGPQVV
                     VGSSMGGWLAL"
     gene            476..1708
                     /locus_tag="VFL49_07700"
     CDS             476..1708
                     /locus_tag="VFL49_07700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7911899.1"
                     /translation="MPYRVVARRAQAAPGSIAVLVVVPALDAGAADAGAVELVRILAS
                     AGFQAIVVSRAGRLVADVTAAGGEFIPLDVSSNNPLLMLRNALKLAQIVRDRRCRVIH
                     AFGRAGAWSAFAAARLCRTPFVTSCYKGFREQNIFKHFYNGVMARGDHVIASSEQIAE
                     LINDRYGTSWNRIAVVSCSIDLDRFDPEKITRERIDAMRGAWGVKRDTKVILITGRIV
                     RRKGHHIVVKAVKRLKEMGLKDFLCVFVGEDRGRTRYTGELWDLVVSTGTMDVIRMAA
                     PVADMPAAYAAASVIVSAAVQPEGVQRAILEAQAMARPVIVSDLGAGTDVVLTAPAVP
                     ESRIAGFRFQSGDDAALAAALLRLFSMPEPNRATIGRRGREWVLGHFNKAVVTEQMLR
                     LYRDIAGPGDQPGAAVPN"
     gene            1894..2421
                     /gene="infC"
                     /locus_tag="VFL49_07705"
     CDS             1894..2421
                     /gene="infC"
                     /locus_tag="VFL49_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7911900.1"
                     /translation="MKSALPVQKDGPRINEEIRVREVQLIDAGGHNHGPTPIQTALDM
                     AQAAGLDLVEIAPNSSPPVCKILDYGKYKFQAQKKAAEARKKQKVVEVKEIKLRPMID
                     DHDYDVKMRSMKRFFEDGDKVKITLRFRGREMAHQELGYKLLERVKADTGPFAKIESE
                     PRFEGRQMVMMLAPR"
     gene            2692..2892
                     /gene="rpmI"
                     /locus_tag="VFL49_07710"
     CDS             2692..2892
                     /gene="rpmI"
                     /locus_tag="VFL49_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7911901.1"
                     /translation="MPKLKTKSGAKKRFKITATGKVKYQQTGKRHGMIKRTTKTIREH
                     RGTNVLFKTDGDNIKKYWLPNG"
     gene            2956..3315
                     /gene="rplT"
                     /locus_tag="VFL49_07715"
     CDS             2956..3315
                     /gene="rplT"
                     /locus_tag="VFL49_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7911902.1"
                     /translation="MARVKRGVTSHAKHKKVLKAAKGYYGRRKNTIRIAKQAVEKANQ
                     YAFRDRKRRKRTFRALWIQRLNAAVRPFGLNYSRFIAGLAKAGVTVDRKVLSDLAIRE
                     PAAFQAIVEKAKAALPA"
     gene            3432..4514
                     /gene="pheS"
                     /locus_tag="VFL49_07720"
     CDS             3432..4514
                     /gene="pheS"
                     /locus_tag="VFL49_07720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7911903.1"
                     /translation="MSDIAKLEQDILASIAAAKDEAVLEAVRIAALGKNGSVSALLKT
                     LGAMSPEERKTQGPVINGIKDRVTESIARRKEALTAAALDARLNTETVDVTLPVREAP
                     AETGRIHPISQVTDELTAIFADMGFAVAEGPDIETDDYNFTKLNFPEGHPAREMHDTF
                     YFAPKPDGSRLLLRTHTSPVQVRTMLSKKPPIRVICPGRTYRSDSDQTHTPMFHQVEG
                     LVIDRGSHLGHLKWILEEFCKAFFEVDSVKMRFRPSFFPFTEPSLEVDIQCKRDKGEI
                     RFGEGEDWLEILGCGMVHPNVLRNCGLDPDEYQGFAWGMGIDRIAMLKYGMNDLRAFF
                     EADVRWLSHYGFRPLDFPTLAGGLSS"
     gene            4511..5002
                     /locus_tag="VFL49_07725"
     CDS             4511..5002
                     /locus_tag="VFL49_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911904.1"
                     /translation="MTAPQSSPLDRAFFALVTRGWDTRKIAGYESTLRKRDKEMSAEA
                     SHTFASIDAKATGLLTHTSMMIAGLGLIAPLVADSHLEVGIVVAEMAIYLLIALGCLR
                     CLSVFRAHEFDIDDHFRDIIHRELIIRAELYSLCIRVAILFTIVVFILLPVLYFWTPG
                     KSA"
     gene            4999..7413
                     /gene="pheT"
                     /locus_tag="VFL49_07730"
     CDS             4999..7413
                     /gene="pheT"
                     /locus_tag="VFL49_07730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7911905.1"
                     /translation="MKFTLPWLKEHLETDASLAEIVDKLTMIGLEVEKVEDTAEQLKP
                     FVIASVISAEKHPNADRLKVCMVDIGDGKPIQVICGAPNARAGMRGVFSAPGTFIPGK
                     NITLTVGTIRGVESRGMLCSAAELMISDDHEGIIDLPADAPVGKSYVEWAGTTDPVIE
                     INLTPNRPDCTGVSGIARDLGASSIGTYKDKIPKLVKGSFPCPVKVTLDFGKTPSLCP
                     AFGLRLVRGVKNGPSPDWLQKRLTAIGLRPINALVDITNFITFDRGRPLHVFDADKVH
                     GNLTVRRARNEEKLVALDGKTYALDAAICVIADEQGVESLSGIMGGEATGCSDKTTNV
                     LIESALWDELNIAQTGRKLGINSDARYRFERGVDPAFMLPGLELATRMVVDFCGGEAS
                     DVVVAGDPTPKEKIIDFPLSELPRLAGLKLPLADLRRVLEKLGFFAAGHGERVKIAVP
                     SWRPDVHGRADIVEELVRIVGVDRVPSTRFVREQARKPVLTVIQNRTRKAKRALATRG
                     MAEAVTWSFVSKREAELFGGGTPELALANPIAAELSDMRPSLLPGLVMAAQRNADRGI
                     TDTALFEVGQIFKGDKPADQLIAASGLRRGLARPSGIGRHWSDKGGPVDVFDTKGDAI
                     AVLAAAGAPVQALQVVPGGPAWFHPGRSGTIQIGPQNILGYFGELHPRAIAGLKAEGP
                     LVAFEVVVEKIPEPKARPTRAKPLLDLSPFQPVDRDFAFVVDSTVKAADIVRAAQNVD
                     KKLVSNVVVFDVYEGKGIEPGKKSIAIAVTIQPRDKTMTDAEIETLAGKIIAEVSKRT
                     GGVLRG"
     gene            complement(7410..>7616)
                     /locus_tag="VFL49_07735"
     CDS             complement(7410..>7616)
                     /locus_tag="VFL49_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7911906.1"
                     /translation="KTLPAAFGGRRFDRSLLESLPPGIDPCGENGEFHSFVAAGPMLS
                     RRVAVKIGETIERDGFAYTDMSPA"
BASE COUNT         1561 a         2281 c         2325 g         1449 t
ORIGIN      
        1 gagcgcgagc catccgccca tcgacgagcc gacgaccacc tgcgggccgg cgcaacaggc
       61 cgagaacacc gcgaggctct cgtcgagcca gcgcccgagg gtgccggcgc ggaaatcgcc
      121 cccggattca ccatgtccgg aataatcgaa gcggacgcag gcgcggccat tttcacgcgc
      181 ccaggcgctc aaagcctcgg ctttggtccc cttcatgtcg gatttgaagc cgccgagcca
      241 gaaaatcgcg ggattactgc ctgtgcgctc gcgcacggcg atggatcggc cactcactgt
      301 tgaaccgact gtgatggatg tcaggcgcgc ttcactcatg gatgtctttt cgcaggaaga
      361 ccgatgtcat ggcaatgcgc aggcgttgct cagcttgccg gtggagtcta tacctcatcg
      421 ctcagcacca acggatccca tgtcgagttt acccgcaaca gcctccgaac cgagcgtgcc
      481 gtaccgcgtc gttgcgcgaa gggcgcaggc cgcgcctggc tcgatcgcgg tgctggtcgt
      541 ggttcctgct ctcgacgccg gcgcagccga cgccggcgca gtcgaactcg tgcgcattct
      601 cgcgagcgcg ggatttcagg ccattgtcgt gtcgcgcgct ggccgcctgg ttgccgacgt
      661 aactgcggcc ggcggcgaat tcatcccgct cgatgtctcg agcaacaacc ctctcctgat
      721 gctgcgcaac gcactcaagc tcgcgcaaat agttcgggac cgacggtgcc gcgtgatcca
      781 cgcctttggc cgcgctggcg cgtggagcgc atttgccgcg gcgcggttat gccgcacgcc
      841 gtttgtaacg agttgttaca aaggttttcg cgagcaaaac attttcaagc acttttataa
      901 cggtgtgatg gcgcgcggcg atcatgtcat cgcctcgagc gagcagatcg ccgaactgat
      961 caacgatcgt tacggtacct cctggaacag gatcgccgta gtgtcatgca gcattgatct
     1021 cgatcgcttc gaccccgaaa aaattacgcg tgaacgcatc gatgcgatgc ggggcgcatg
     1081 gggcgtcaag cgcgacacga aagtcattct gatcaccggg cgcatcgtgc gccgtaaagg
     1141 tcatcatatt gtcgtcaaag cggtgaagcg gctcaaggaa atgggcttga aggatttcct
     1201 ttgcgtgttc gtcggcgaag atcgcggtcg cacgcgctac acaggcgagc tttgggacct
     1261 ggttgtatcc acgggaacga tggatgtgat tcgcatggct gctccggtcg ccgacatgcc
     1321 ggccgcctat gcagccgcat ccgtcatcgt ctcggcggcg gtacagcccg aaggggtgca
     1381 gcgagcaatc cttgaagcgc aggcgatggc acggccggtc atcgtgtcgg acctcggagc
     1441 cgggaccgat gttgtactca cagcgcccgc ggtgcccgag agccggattg ccggatttcg
     1501 ctttcagtcc ggcgacgacg ccgcactggc ggccgccctg cttcggctgt tctcgatgcc
     1561 tgaaccaaat cgggcaacca ttggccgccg cggccgcgaa tgggtgctgg gacatttcaa
     1621 taaggctgtt gtgaccgagc aaatgttgcg cctgtatcgg gacattgccg ggccaggcga
     1681 ccagccgggt gccgccgtgc caaactaact cacgacaaaa taagagcaat ttgaacaggt
     1741 tgaccttcga tttcgaaccg ttaaccgcca atacaagcgg aactgcgttg acttctcggc
     1801 cgtttctacc aatctacggc tattccgaag gcgcactctg acgtcggcgg acagttgcaa
     1861 cagcacgagg agaaattagc cattcgtcgt ccgatgaagt ccgcgctgcc cgttcaaaag
     1921 gacggcccgc gcataaacga ggagatccgc gtccgcgagg tccagcttat tgatgccggt
     1981 ggtcacaacc acggtcccac accgatccaa accgcactcg acatggcgca ggctgcgggg
     2041 ctcgacctgg tcgaaattgc ccctaactca tcgccccccg tctgcaagat cctcgactac
     2101 ggcaagtaca aattccaggc gcaaaaaaag gcggcagagg ctcgaaaaaa gcaaaaggtc
     2161 gtcgaggtca aggagatcaa actccggccg atgatcgacg accacgatta tgacgtgaaa
     2221 atgcgctcta tgaagcggtt cttcgaggac ggcgacaagg tgaaaatcac ccttcgtttc
     2281 cgcggccgcg aaatggcgca tcaggaactc ggctacaaat tgctcgaacg cgtgaaggcg
     2341 gacaccggtc cgttcgccaa gattgaatcg gagccgcgct tcgagggccg gcaaatggtc
     2401 atgatgctgg cgccgcgcta gcgcggcagg tgcattactc gtaagtcaag cgcgccgccc
     2461 gcaaattgtg ggcggcgcgc tagctttttg tgccgtttag ccttacggcg acgggtggtt
     2521 gcgtaggcga gcggcctctg ccataacgcc gcgcattcgc cgcccggcca gtaagggctg
     2581 ccgtggcggc gattaacgct caatcccgag cacccgctcc aggcgccgac atgcgctcaa
     2641 ggagcatatt ccggccggcg gccacacccg cacgccgatg aggagagcca aatgcccaag
     2701 ttgaagacca aatcaggcgc taaaaagcgc ttcaagatca ccgcgaccgg caaggtgaaa
     2761 taccagcaga ccggtaagcg tcacggcatg atcaagcgga ccaccaagac gatccgcgag
     2821 catcgcggca ccaacgtgct gttcaagacc gacggcgaca acatcaagaa atactggctg
     2881 ccgaacggct gaccggattt cttcggcgcg gctgttcttc gtccgcatct gcacgtattt
     2941 gcaaggagat tttcgatggc ccgcgtcaaa cgtggtgtca cttcacacgc caagcacaaa
     3001 aaagtcctga aggccgccaa gggctactac ggccgccgca agaacacgat ccgcatcgcc
     3061 aagcaggcgg tggaaaaggc aaaccagtat gcctttcgtg atcggaagcg gcgcaagcgc
     3121 acattccgtg cgttgtggat ccagcggctg aatgcggcgg tgcggccctt tggattgaac
     3181 tacagccgtt tcatcgcggg gctcgcaaaa gccggcgtga ccgtggaccg aaaggttttg
     3241 tccgatcttg cgatccgcga gcctgccgct ttccaggcaa tcgtggaaaa ggctaaagcc
     3301 gcgttgccgg cctgaagttt caacgtcgtc atgcccggcc ttgtgccggg catccacgac
     3361 ttgcttcgtg taccgaaggc gtggatggcg ggacaggccc ggccatgacg aggcacagga
     3421 tagccctttc catgtccgac attgcaaagc ttgaacagga cattctggcg agtatcgccg
     3481 cggcaaaaga cgaagcggtg ctcgaagctg ttcgcattgc cgcgcttgga aagaacggct
     3541 ccgtttcggc gctgctcaag acgcttggtg cgatgtcgcc ggaggaaagg aagacgcaag
     3601 gcccggttat caacggcatt aaggatcggg tgacggaatc gatcgcccgg cgcaaagagg
     3661 cactcaccgc ggcggcactc gatgcgaggc ttaacacaga aacggtcgac gtcacgctgc
     3721 ctgtgcgcga agctccggcg gagaccggtc gcatccatcc gatcagccag gtcacagatg
     3781 agttgacggc cattttcgcc gacatgggct tcgcggtcgc ggaaggtccc gacatcgaaa
     3841 ctgacgacta caatttcacc aaactgaact ttcctgaagg ccacccggcg cgcgagatgc
     3901 acgatacatt ctatttcgca ccgaagccgg acggatcgcg cctgctgctg cgtacgcata
     3961 cctcgccggt tcaggtgcgc acgatgctca gtaagaagcc gccgatccgc gtgatttgtc
     4021 cggggcgcac ctatcgcagc gactcggacc agacccacac gccgatgttc catcaggtgg
     4081 agggcctcgt aatagacaga ggctcccatc tcggtcacct gaagtggatc cttgaggaat
     4141 tctgcaaggc cttttttgag gtcgacagcg tgaagatgcg cttccggcca tcgttcttcc
     4201 cgtttacgga gccgtcgctc gaggtcgata ttcagtgcaa gcgcgacaaa ggtgaaatcc
     4261 gtttcggcga aggcgaggac tggcttgaaa tcctcggctg cgggatggtc cacccgaacg
     4321 tcctgcgcaa ttgcgggctc gatccggacg agtatcaggg ctttgcctgg ggaatgggca
     4381 tcgaccgcat cgcgatgctc aagtatggca tgaacgacct gcgcgccttc ttcgaggccg
     4441 atgtgcgctg gctttcgcac tatggtttcc gtcccctcga ctttccgacg ctggcgggag
     4501 gtctgagctc atgaccgcgc cgcagtcctc gccgctcgat cgcgcattct tcgcgttggt
     4561 cacgcgcggc tgggatacgc gcaagatcgc gggttacgaa tcgaccctcc gcaagcgcga
     4621 caaggagatg tcggctgagg cttcgcacac attcgcctcg atcgatgcca aggccaccgg
     4681 cctgctgaca cacacgtcga tgatgatcgc cggcctcggg ctgatagcac cgcttgttgc
     4741 cgacagccac cttgaggttg gcatcgttgt cgcagagatg gcgatatatc tgctcatcgc
     4801 gcttggctgc ctgcgatgcc tgtcggtatt ccgggcgcac gagttcgaca tcgacgatca
     4861 tttccgcgac atcatccatc gtgaactgat catccgtgcg gagctttaca gcctctgtat
     4921 ccgggtggcg atcctgttca cgatcgtcgt attcatcctg ctgccggttc tctatttctg
     4981 gacaccgggg aaatcggcat gaaattcacc ctgccatggc tgaaagagca tctggagacc
     5041 gatgcgtcgc tggcggagat cgtcgacaag ctcacgatga tcggcctcga ggttgagaaa
     5101 gtcgaggaca cggctgagca gctgaagcca ttcgtgattg catccgtcat ctcggccgaa
     5161 aagcatccca acgccgatcg tctcaaggtc tgcatggtcg atatcggcga cggcaagccg
     5221 atccaggtca tctgcggcgc gcccaatgcg cgtgcgggca tgaggggtgt cttctcggcg
     5281 ccaggaacat ttatcccagg aaaaaatatc acgctgacgg tcggcacaat ccgcggcgtg
     5341 gaaagccgcg gcatgctgtg ctcggccgcc gagctgatga tttccgacga ccatgaaggg
     5401 atcatcgact tgccggcgga tgcgccggtc ggcaaatcct atgtagaatg ggcgggcaca
     5461 accgacccgg tcatcgaaat caaccttacg cccaatcgcc cggactgcac cggcgtttca
     5521 ggcattgcgc gcgacctcgg cgcaagctcg atcggcacat acaaagacaa gattccaaaa
     5581 ttggtgaagg gctcgttccc ctgcccggta aaagtcaccc tcgacttcgg caagacgccg
     5641 tcgctgtgcc cagctttcgg attgcgcctc gtgcgcggcg tcaagaatgg gccttcgccc
     5701 gactggctgc aaaagcggtt gaccgcaatt gggctgcggc caatcaacgc tctcgtcgat
     5761 atcactaatt tcatcacctt cgaccgcggt cggccgttgc acgtattcga tgccgacaag
     5821 gttcacggca atctgacagt gcggcgcgca agaaatgaag aaaagctggt ggcgcttgac
     5881 ggcaagacct acgccctcga cgcagccatt tgcgtaatcg cggacgaaca aggcgtggag
     5941 tccttgtccg gcatcatggg tggcgaggca accggctgct cggacaagac tacaaacgtg
     6001 cttatcgaat cggcgttatg ggacgagctc aatatcgcac agaccggacg caagctcggc
     6061 atcaattccg atgcgcggta ccgcttcgag cgtggtgtcg atcccgcgtt catgttgccc
     6121 ggcctcgaac tcgctacccg gatggtggtc gacttctgcg gcggcgaagc gtccgacgtt
     6181 gtcgtcgctg gcgatccgac gccgaaggaa aaaatcatcg actttccact gagcgaattg
     6241 ccgcggcttg ccgggctgaa actgccgctg gcggatttgc ggcgtgtcct ggagaagctc
     6301 ggctttttcg ctgccggaca cggcgagcgc gtgaaaatcg ccgtgccatc ctggcggccg
     6361 gatgttcatg gtcgcgccga tattgtcgag gaacttgtac gtattgtcgg ggtggaccgt
     6421 gtgccgtcga cgcgttttgt gcgagaacag gcgcgcaagc cggtgcttac ggtgatccag
     6481 aaccgcacac gtaaggcaaa acgcgcgctt gccacgcgcg ggatggcgga ggccgtcacg
     6541 tggtcgttcg tgtcgaaacg ggaggccgag ttgttcggcg gcggcacgcc cgagctcgcg
     6601 ctggcgaatc cgattgccgc cgagctatcc gacatgcgcc ccagcctgct ccccggtttg
     6661 gtcatggccg ctcaacgcaa cgccgatcgc ggaattaccg ataccgcgct gttcgaagtc
     6721 ggccagatct tcaaaggcga caagccggcc gatcagctca ttgctgccag cgggttgcgc
     6781 cgtggtcttg ccaggccgtc gggaattggt cgccactggt cggacaaggg cgggcccgtc
     6841 gatgttttcg acaccaaagg cgatgctatt gctgtgctgg ccgccgccgg ggcaccagta
     6901 caggcactgc aagtcgtgcc aggcggccca gcctggttcc atcccgggcg cagcgggacg
     6961 attcagattg gcccgcagaa catcctgggg tacttcggcg aactgcatcc gcgggcgatc
     7021 gccgggttga aggccgaggg tccgttggtt gcctttgaag tagttgtcga aaaaattccc
     7081 gaaccgaaag cgcgtccgac ccgcgcaaag cctctgctcg atttgtcgcc gttccagccg
     7141 gtcgatcgcg attttgcttt cgtcgtcgac agcaccgtga aggcggctga cattgtgcgc
     7201 gcggcgcaaa acgtggacaa gaagcttgta agcaatgtcg ttgtcttcga cgtatacgaa
     7261 ggcaagggca tcgagcccgg caagaaatcg attgcgatag cggtcaccat tcagccgcgc
     7321 gacaaaacga tgaccgacgc tgagatcgag acgctggcgg gtaagatcat agccgaagtg
     7381 agcaagagaa ccggcggcgt attgcgcggt taggccggac tcatatccgt ataggcaaat
     7441 ccgtcacgct cgatggtttc gccgattttg acggcgactc tccgcgacag cattggaccg
     7501 gctgcaacga aagagtggaa ttcaccgttc tcaccgcatg ggtcgatgcc tggagggagc
     7561 gattcaagca gcgaacggtc gaaacggcgc ccgccgaatg ctgccggcag cgtctt
//
LOCUS       DASSRL010000378         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_692745, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000378 DASSRL010000000
VERSION     DASSRL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692745"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VFL49_07740"
     CDS             complement(<1..1089)
                     /locus_tag="VFL49_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET7911907.1"
                     /translation="MIGSLLRPAKLKEARTRYDQGLISLEELSGIEDRAVRDAVEIQE
                     GAGLAVVTDGEYRRLNFQDSFGESVAGYDAGRPTLEFYEKRVEGGNPLQRWEIPDQGE
                     RKGTAVAQRRPVAEKIRLARNVPAEEFDFLRRAARKPAKVALIGPDRIAQRFDWQNSK
                     AVYSGLDEFVADVVQVEREIIRGLVDAGCHYVQIDAPGYTAYVDAPSLEAMRRRGEEP
                     QENFNRSLRADNAVIEDFDDVTFGIHLCRGNQRSMWHREGSYDEIAERLLNELKHDRF
                     LFEYDTPRAGGFEPLRFLPKGKIVVLGLVSTKVPRLETVDELKRRLDEASKYAPLEQL
                     AISPQCGFSSDVVGNLITEDDQKRKLEVV"
     gene            complement(1124..1882)
                     /locus_tag="VFL49_07745"
     CDS             complement(1124..1882)
                     /locus_tag="VFL49_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HET7911908.1"
                     /translation="MKFHPGIFFALVISSSLLGACAQHTAGPGPSVRRAHGIYHVVKP
                     GENLFRIGKAYDIGFEDLARVNGIRDPGQIRVGQRILIPGATRALPVEIIAPVEIGPP
                     SRSVAPAVEPMEEALLWPINGSINSGYGARGAGFHDGIDIGAPEGTPIHAVEKGEVIY
                     SDQIRGYGNIVIVRHGGGIVSVYAHNQVNLVREGQNVDRGEVIAKVGSTGRVTGPHLH
                     FEIRKHNAAQDPLRYLPQLCCVPASDNVAPKGSD"
     gene            complement(1977..>2631)
                     /locus_tag="VFL49_07750"
     CDS             complement(1977..>2631)
                     /locus_tag="VFL49_07750"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET7911909.1"
                     /translation="EPSPHDFVWARERMVQEQIIARGVNDTRVIAALRKIPRHLFVDP
                     GIVNRAYHDSALPIGEKQTLSQPYMAARMTEALCLTGDEKVLEVGTGSGYQTALLAEL
                     CFNVFSVEKIRALSHKARALLDQLGYQNIALHVGDGTIGWSEHAPYDAIIVSAGAPST
                     PRPLLDQLAVRGRLVIPVGDEQGQMLLRVTRTRSGFKQEQLGECKFVKLFGKYGWRH"
BASE COUNT          504 a          860 c          749 g          518 t
ORIGIN      
        1 aactacttcg agcttgcgtt tctggtcgtc ttcggtgatc aagttaccga ccacgtccga
       61 agagaaaccg cactgggggc tgatggcgag ttgctcgagg ggcgcgtact tgctcgcttc
      121 gtcgaggcgc cgcttgagct cgtcgaccgt ttccaaccgc ggcaccttgg tgctcaccag
      181 gccaagcacc acgatctttc ccttgggcaa aaagcgcagc ggctcgaatc cgcccgcgcg
      241 cggcgtatcg tactcgaaca ggaaacggtc atgcttcaat tcgttcagca gccgctcggc
      301 gatctcatcg tagctgcctt cgcggtgcca catgctgcgc tggttgccgc ggcaaaggtg
      361 aatgccgaac gtcacatcgt cgaagtcctc gatcaccgcg ttatcggccc gcagagagcg
      421 gttgaaattc tcctgcggct cttcgccgcg gcggcgcatc gcttccagcg acggcgcatc
      481 gacataagcg gtgtaacccg gcgcatcgat ctgcacgtaa tgacagccgg cgtcgaccaa
      541 accgcggatg atttcccgct ccacctgcac tacatcggca acgaactcat ccagtccgga
      601 ataaaccgct ttggaatttt gccaatcgaa ccgctgggcg atgcggtcgg gaccgatgag
      661 cgcgactttg gcgggtttgc gcgcggctcg gcgcagaaaa tcgaattcct ccgccggcac
      721 gttgcgcgcc aggcgaattt tttccgccac cggccggcgc tgcgccacgg cggttccttt
      781 gcgctcgccc tgatcgggaa tttcccaacg ctgcagcggg ttgccgccct cgacccgttt
      841 ctcatagaac tccagcgtcg gccgtccggc gtcgtaaccg gccaccgatt cgccgaagct
      901 gtcctgaaaa ttcaagcgcc ggtactcgcc gtcggtgaca acggccaaac cggctccctc
      961 ctggatctcg acggcgtcgc gcaccgcacg gtcttcaatg ccggacaatt cttcgaggga
     1021 gatcaagcct tggtcgtaac gcgtccgcgc ttcttttaac ttggccggac gcaagaggct
     1081 gccaatcacg tcgtttctga ttctcgacaa taatttcatt caatcaatcg ctacctttcg
     1141 gcgcaacatt atcggatgcc ggaacgcagc acaactgcgg cagatagcgc agcggatcct
     1201 gagccgcatt gtgcttgcgg atctcgaagt gaagatgagg accggtgacg cgccccgtgc
     1261 tgcccacttt ggcgatcacc tcgccgcgat cgacgttttg cccctcgcgt acgaggttga
     1321 cctgattgtg cgcatagacc gatacgatcc cgccgccgtg gcgcacgatg acgatgttgc
     1381 cgtagccgcg gatctgatcg ctgtaaataa cttcgccctt ttccaccgcg tgaatcggcg
     1441 tgccctccgg cgcgccgata tcgatgccgt catggaatcc cgcgccccgc gccccatatc
     1501 ccgagttgat tgaaccgttg atcggccaga gcaacgcttc ctccatcggc tccactgccg
     1561 gcgcgacgct gcggcttggc ggcccgattt ccaccggcgc aatgatttcc accggcagcg
     1621 cgcgggtagc gccgggaatc agaatccgtt gtccgacgcg aatctggccc ggatcacgga
     1681 tgccgttcac acgcgccaga tcctcgaacc cgatgtcata agccttgccg atgcgaaata
     1741 gattctctcc gggcttgacc acgtgataga tgccgtgcgc ccgcctcacg ctcggccccg
     1801 ggccggccgt gtgctgcgcg cacgctccca gaagcgacga cgaaatgacg agcgcaaaaa
     1861 aaatcccggg atggaatttc attgctgacg ttgcggtcgt agtcagcccg aacaaaaaaa
     1921 ataattcgcc gctcgacccg cctagtccga tcgaaggcag cgcgccgccg ccaagctcag
     1981 tggcgccagc cgtacttgcc aaacaacttg acaaacttgc actcgccgag ctgctcttgt
     2041 ttaaatccgg accgcgtccg ggtcacgcgc agcagcatct ggccttgctc atcgcccacc
     2101 ggaatcacca agcggccgcg cacggcgagc tggtcgagca gcggtcgggg agtagacggc
     2161 gctccggcgc tgacgatgat cgcatcatac ggcgcgtgct ccgaccagcc gatggtcccg
     2221 tcgccgacgt gaagcgcgat attttgatag ccgagctgat ccagcaatgc cctcgcttta
     2281 tgcgacagcg cgcgaatctt ctccacggaa aagacattga aacaaagctc tgcgagcagc
     2341 gcggtttggt aacccgagcc ggttccgacc tccagcactt tttcatcgcc ggtcaggcac
     2401 agcgcttcgg tcatccgggc cgccatataa ggctgggaca gggtctgctt ttcaccgatc
     2461 ggcaaagcgc tgtcgtggta cgcccgattg acgatccccg gatcgacgaa caggtgcctg
     2521 ggaattttac gcagcgcggc gatcacccgg gtgtcattga cgccgcgagc gatgatctgc
     2581 tcctgaacca tgcgctctct ggcccagaca aaatcatggg gacttggttc g
//
LOCUS       DASSRL010000379         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_814830, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000379 DASSRL010000000
VERSION     DASSRL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814830"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFL49_07755"
     CDS             complement(<1..268)
                     /locus_tag="VFL49_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911910.1"
                     /translation="MSNDSEIDYSGVDYAAITSKAISQPLVTHIYTADPSAHVFEGRI
                     YIYPSHDVEAGIPFNDNGDHFDMRDYHVLRMDSPTAPAVDCGLAL"
     gene            complement(316..1791)
                     /locus_tag="VFL49_07760"
     CDS             complement(316..1791)
                     /locus_tag="VFL49_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7911911.1"
                     /translation="MTTNTERLSVGEKVGYSLGDLAANLIFQTFVTFLAFFYTDVFKI
                     PAGAASAIIFWGGVIGGVVFNPIMGLIADRTNTRWGKFRPWVLWTAVPFGVLSLLAFS
                     TPDLGEQGKIYYAMITYTLLVIIYAANNLPYAALSGVLTGNMSERNSLSSYRFVAVMV
                     AQFIIQVLLLPLVLMLGHGDQAVGFRKAIMIFAIIGTIFFVITFLTTKERVVPTPEQK
                     SSVREDLTDLVRNKPWLLMLAVTILVFITLALKGGSYIYYFQNYVDQAALAQFLDGIG
                     FNGFIGGLNSFVTGIGLGEFHWPDDAVASGFGIFNGGGIIFMIVGIGFSKPLADRFGK
                     RNVFGVALLLATLCLIAFVFYPPTAVGTIFVTQILHGFFYGITIPLLWAMTADVADYS
                     EWKNNRRATAIIFSAMILGLKFGLAVGGALVAKLLDVYGYVRGAEEQAPAVVHGIKLA
                     ISVYASVPFLICCGLLFFYEINKAMETRIESELGQRRGAAS"
     gene            2226..3062
                     /locus_tag="VFL49_07765"
     CDS             2226..3062
                     /locus_tag="VFL49_07765"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7911912.1"
                     /translation="MEQAIPADALDDEGGTALLETLQGKVQLPRGVEAVRESYRNARP
                     FPHAVIDNLFSPTLLDRVITEAPGKDQWVRIDHGLQRVERMRSAVELGPASTELISVL
                     HSAAFLYLLSEITGVWQLLPDPYLQGGGHALMHRGGFFQIHADRNVAYETGLRRRLAL
                     IIFLNKDWPREYGGQLELWDDQATRCEVSIDPVYNRTALFEVAYPNYHGVPAPLACPR
                     DRFRHSFLVYYHTAGAGDNVTPHASVFAPHFYQKKPSLVERAARQLMPPLMVRALKAL
                     RR"
     gene            complement(3195..>4183)
                     /locus_tag="VFL49_07770"
     CDS             complement(3195..>4183)
                     /locus_tag="VFL49_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006643166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HET7911913.1"
                     /translation="GKAADIKGRIESIRQQVEEATSDYDKEKLQERVAKLSGGVAVIK
                     VGAATEIEMKEKKARVEDALHATRAAVEEGVVPGGGVALIRAQKALEKATLNDLGEAK
                     KIVVEKENTTVIDGKGKNADIKARVEQIRKQIEEATSDYDKEKLQERVAKLSGGVAVI
                     KVGAATEIEMKEKKARVEDALHATRAAVEEGVVPGGGVALIRAQKAVEKLEAKNEDQA
                     FGIKILARSIEEPLRQIVTNAGEDAAVVLAKVKEGKGTFGYNAATSSYGDMLELGILD
                     PTKVTRLALQNAASVAGLLLTTEVMIAEAPKEEADHAHGAPGGMGGMGGMDM"
BASE COUNT          806 a         1303 c         1235 g          839 t
ORIGIN      
        1 cgagtgcgag cccgcagtcg accgccggag ctgtcggcga atccatccgc agcacgtgat
       61 agtcacgcat gtcgaaatga tcgccgttgt cgttgaacgg gattccggct tcgacatcat
      121 gcgagggata gatgtagatc ctgccctcga acacatgcgc cgacggatcg gccgtatata
      181 tgtgtgtcac cagcggctgg gaaatcgctt tgctggtgat agccgcgtag tcgacgccgg
      241 aataatcgat ctctgaatcg ttcgacatga tgctcaggtt tctcgtgaat gaatttgccg
      301 tcgtgcgtga ctgcgtcagg aggccgcgcc gcgacgctga cccagctcgc tctcgatgcg
      361 tgtttccatt gccttgttga tctcgtagaa gaacagcagg ccgcagcaga tgagaaaggg
      421 aaccgacgca tagacgctga tggccagctt gatgccgtgc acaaccgcgg gggcctgctc
      481 ttccgcaccg cgcacatagc cgtaaacatc cagcagcttc gccaccaacg caccgcccac
      541 ggccagtccg aacttcagtc ccaggatcat cgcggagaag ataatagcgg tcgcgcggcg
      601 gttgttcttc cactcggagt agtcggccac atcggcagtc atggcccaca gcagcggaat
      661 cgtgatgccg tagaaaaagc cgtgcaggat ctgcgtaacg aagatcgtgc cgaccgcggt
      721 gggcggatag aaaacgaacg caatcaggca aagggtcgcc agcaacagcg ccacgccaaa
      781 cacattgcgc ttgccgaagc gatcggccag cggtttggag aagccgatgc cgacgatcat
      841 gaaaatgatg ccgccgccat tgaaaatgcc gaagccggaa gcgacagcgt cgtcaggcca
      901 gtgaaactcg cccaggccga tgccggtaac gaacgagttc aacccgccga tgaatccgtt
      961 gaatccgatg ccgtccagga actgcgccag tgcagcctga tcgacatagt tctggaagta
     1021 atagatgtag gaaccgccct tgagcgccag cgtgatgaac accaggatgg tgaccgcaag
     1081 catcagcagc cagggcttgt tgcgcaccag atccgtcagg tcctcgcgca ccgatgattt
     1141 ctgttcgggc gtgggcacga cacgttcttt cgtcgtcagg aacgtaatca cgaagaagat
     1201 cgtgccgatg atcgcgaaga tcatgatcgc cttgcggaag ccaaccgcct gatcgccgtg
     1261 gccgagcatg agtaccagcg gcagcagcag cacctgaatg atgaactgcg ccaccatcac
     1321 tgccacaaaa cggtacgacg acagactgtt gcgctcggac atgttgccgg tgagcacgcc
     1381 gctcagcgcg gcatagggca gattgttggc cgcataaatg atcaccagca gggtgtaggt
     1441 gatcattgcg taatagatct tgccctgctc gccgaggtcg ggggtcgaga aggccagcag
     1501 cgagagcaca ccgaagggca cggccgtcca cagcacccag gggcggaact tgccccaacg
     1561 cgtgttggta cggtcggcaa tcagtcccat gatgggattg aagacgacgc cgccgatgac
     1621 tccgccccag aaaatgatcg ccgacgcagc gcctgccggg atcttgaaaa catcggtata
     1681 gaagaaggcg agaaaggtaa cgaaagtctg gaagatcagg ttggcggcca ggtcaccgag
     1741 actgtatccg accttttccc ccacactcag gcgttctgta tttgtcgtca tgttaccgct
     1801 ctcatggttt tatcggcggg cggagaataa acggcattcc gggactcggc cagtgcgcag
     1861 cgccttactt cattgaacca cggacgattt ccgcggcccg cgttgccctg gacagaagcc
     1921 cccacccgtt taccccctgt atggcgcgtg cacccggaac gtcccggtgc atccggcatc
     1981 tcattggcga tccaaagggt gcatgcccct tcccgcatgg cgacccggca ggcatgccac
     2041 ataatgtggt gcgctcggac aatcgtgctg ttcgccgcgt tttcatggac ctttcgctag
     2101 ttcgatgcgt ttgttcggcg gccaaatcga gatcgactgt aatgaaattc gacgatcttt
     2161 gtgcggcatg tctcttgctt tgaatatcag cgcgcggcag cacccgcgga acaacgacca
     2221 gcctcatgga acaagcgatc ccggcggatg cccttgacga cgagggcggc accgcactgc
     2281 tcgaaaccct gcagggcaaa gttcagttgc cgcgaggggt cgaggctgtg cgcgaaagct
     2341 atcgcaacgc cagaccgttt cctcatgcgg tcatcgacaa tcttttttcc ccgaccctgc
     2401 tggatcgggt gatcaccgaa gcaccgggca aagatcagtg ggttcggatc gatcatggac
     2461 tgcagcgagt cgagcgcatg cgctcggcgg tcgagctggg tcccgccagc actgagctga
     2521 tctcggtcct gcactcggcg gcgtttcttt acctgctctc cgagatcacc ggagtgtggc
     2581 aactgctgcc ggatccgtat ctccagggcg gcggccacgc actgatgcat cgcggcggtt
     2641 tcttccagat tcacgccgat cgcaacgtgg cttacgagac cgggctgcgc cgccgactgg
     2701 cgctgatcat tttcctcaac aaggactggc ccagggaata cggcggtcag ctggaactgt
     2761 gggatgacca ggcaacgcgc tgtgaagtat cgatcgaccc ggtctataac cgtacggcgt
     2821 tgttcgaagt cgcctacccg aattatcacg gcgtacctgc gccactggcc tgtccgcgtg
     2881 atcggttccg gcactcgttt ctcgtgtact accacacagc cggtgccggc gataacgtga
     2941 ccccgcacgc ctcggtgttc gcaccgcatt tctatcagaa gaagccatcg ctggttgaac
     3001 gcgctgcccg gcaactgatg ccgccgctca tggtccgcgc attgaaagcg ctgcgtcgct
     3061 gatcccggac gcgtcgcact cccgcaagta aacgatcgcc caaaaagaaa gccccgcttg
     3121 cgcggggctt tctccgactt ccggttccgg cgctggccag cagaccagtc agccggttgg
     3181 ccagtcggcc cgaatcacat atccattccg cccatgccac ccatgccgcc gggggcgcca
     3241 tgagcgtggt cggcttcttc cttcggagct tcggcgatca tcacctcggt cgtcaggagc
     3301 aggccggcaa ccgacgctgc gttctgcagt gccaggcgcg tgaccttggt cggatccagg
     3361 atgccgagtt cgagcatgtc gccgtagctg ctggtcgcag cgttgtagcc gaacgtgccc
     3421 ttgccttcct tgaccttcgc cagcacgacc gcggcatctt caccggcgtt cgtgacgatc
     3481 tggcgcagcg gctcttcgat ggaacgggcc aggatcttga tgccgaacgc ctggtcttcg
     3541 ttcttcgctt cgagcttctc gacagccttc tgagcgcgga tcagcgcgac accgccgccc
     3601 ggcaccacac cttcttcaac ggcggcacgg gttgcgtgca gcgcgtcttc gacgcgggcc
     3661 ttcttttcct tcatttcgat ttcggtcgca gcaccgacct tgatcacggc gacgccgccg
     3721 gagagcttgg caacgcgctc ctgcagcttc tccttgtcgt agtcggaagt ggcttcttcg
     3781 atctgcttgc ggatctgctc gacacgcgcc ttgatgtcgg cgttcttgcc cttgccgtcg
     3841 atgacggtgg tgttttcctt ctcgacaacg atcttcttgg cttcgccgag atcgttgagc
     3901 gtggccttct cgagcgcctt ctgcgcacgg atcagcgcga caccgccgcc cgggaccacg
     3961 ccttcttcaa cggcggcacg cgtggcgtgc agcgcgtctt cgacgcgggc cttcttttcc
     4021 ttcatctcga tctcggtggc agcgccgacc ttgatcacgg cgacgccgcc ggagagcttg
     4081 gcgacacgct cctgcagctt ctccttgtcg tagtcgctgg tggcttcttc cacctgctgg
     4141 cggatcgact cgatgcgacc cttgatgtcc gcggccttgc cgg
//
LOCUS       DASSRL010000380         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1269883, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000380 DASSRL010000000
VERSION     DASSRL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269883"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1167)
                     /gene="cax"
                     /locus_tag="VFL49_07775"
     CDS             complement(73..1167)
                     /gene="cax"
                     /locus_tag="VFL49_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003589375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HET7911914.1"
                     /translation="MTPLLNDIRNTPLMWLLVLVPVVLAAQLLKPEAHTLLFILSVLA
                     IVPLAALLSRATESVASRTGDMIGGLLNATLGNLTELLIALAALRAGQYVLVKASIAG
                     AIVTNTLFMLGVSFLLGGLKHHVQDFNRNSARLQAGLLFLATIAILVPSVVGEPEAGA
                     TSEFTQTLSVSLAVALIVTYGLGLLFSLRTHREFFAGADHGGHDEEQWSMGLALATLA
                     VVTVLVALVSEVFVESVQQAAKVLGLSSAFVGFIIVALVGGAAEMTAAFSAARKNRLD
                     LSVGIALGSASQIALFVAPVLVLLSYVLGPSPMSLQFWPGAVAMMLIASLTASLVTNT
                     GRSAWFIGCLVTVVYMIFALTLYLLPPRVH"
     gene            complement(1369..1863)
                     /gene="pal"
                     /locus_tag="VFL49_07780"
     CDS             complement(1369..1863)
                     /gene="pal"
                     /locus_tag="VFL49_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET7911915.1"
                     /translation="MIDMRKTLHVARFAAVLVAALAISACAKNAEDAQANAAGQATPG
                     SQQDFVVNVGDRVFFETDSSELTPQSRATLDKQAQWLTNYSQYSQFTIEGHADERGTR
                     EYNIALGARRAQAVRDYLISRGIQAQRMRTISYGKERPVAVCNDISCWSQNRRAVTVL
                     GASS"
     gene            complement(2046..>2946)
                     /gene="tolB"
                     /locus_tag="VFL49_07785"
     CDS             complement(2046..>2946)
                     /gene="tolB"
                     /locus_tag="VFL49_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET7911916.1"
                     /translation="QYFTTPDNWRRIAHIISDAIYERLTGEKGYFDSRIVFVDETGAK
                     ERRVKRLAIMDQDGAAVRYLTRGDDLVLTPRFNPSTQEITYMSYGQGDPRVYLLNIET
                     GQREIVGNFPGMTFAPRFSPDGQRVIMSLQQGGNANLFVMDLRSKQTTRLTDTPAIDT
                     APCYSPDGSRICFESDRGGSQQIYVMSASGGPAQRISFGEGHHSTPVWSPRGDYIAFT
                     GQARGQFSIGILKPDGSGERVLTQGFHNEGPTFAPNGRVLMFFRDVGQGPSLYTIDIT
                     GRNELKVPTPSFASDPAWSPLLS"
BASE COUNT          621 a          895 c          907 g          523 t
ORIGIN      
        1 accgcaccac caaaaagaaa ttgccgtagc atttggcccc caggcatttc acgcgcgccg
       61 atgtaactcc aatcagtgta cccgcggagg cagcagatag agcgtgagtg caaagatcat
      121 gtacacgaca gtaacgaggc atccgatgaa ccacgctgag cggccggtat tggtcaccag
      181 cgacgccgtg agactcgcga tcagcatcat tgccaccgca ccaggccaga actgcagact
      241 catcggcgaa gggccaagca cgtaactgag cagcaccagt accggcgcga cgaagagcgc
      301 aatctgcgag gcgctcccca gcgcaatgcc gacgctgaga tcgagcctgt tcttgcgcgc
      361 ggcggagaac gcggctgtca tttcggcggc gccgccaacg agcgccacga tgatgaatcc
      421 gacgaaggcc gacgaaagcc cgagaacttt cgccgcctgc tgcaccgatt ccacaaacac
      481 ctcgctgacc agcgcgacca gcacggtgac gaccgcaagt gttgcgagcg caagtcccat
      541 cgaccattgc tcttcatcgt ggccgccatg gtcggcgccg gcaaagaact cgcgatgggt
      601 gcgcagcgag aacagcagtc ccaggccata ggtgacgatg agcgccactg caaggctcac
      661 actgagcgtc tgcgtgaatt cggaggttgc gccggcctcc ggctcgccga cgacggacgg
      721 aacgaggatc gcgatggttg cgagaaaaag caacccggcc tgcaaccgcg cgctgttacg
      781 gttgaaatcc tggacgtgat gcttcaatcc gccgagcagg aaggacacgc ccagcatgaa
      841 cagggtgttg gtgacgatcg cacctgcgat cgatgccttc accagcacgt attgcccggc
      901 gcgtagggcc gcgagcgcga tcagaagctc ggtcaggttg ccaagcgtcg cgttgagcag
      961 gccaccgatc atgtcgcctg ttcgcgacgc gactgattcc gtcgcgcggc tcaggagcgc
     1021 cgccagaggc actatggcga gcaccgacag gatgaaaagg agcgtatggg cttcgggctt
     1081 caggagctgt gcggcaagga cgaccgggac caataccagc agccacatga gcggcgtgtt
     1141 gcgaatgtca ttgagcaaag gtgtcatgcc gtccccccgt cccgagaagg catcatagca
     1201 cggcccgaag cgagcacctg ccgtgccgga ttttgaaatc ccccagcaag aacacctgag
     1261 tggcaatcaa tctcggctat aggttttagg cgaaatcggc actacaggcc ggaataggcg
     1321 acggcggccg aatgggccgc cgtctcatca ctcagatgca ttccggactt acgaactggc
     1381 gccgagtacc gtcacggcac gacggttctg tgaccagcac gaaatatcgt tgcagacagc
     1441 caccggccgc tccttcccgt aggagatcgt gcgcatgcgc tgcgcctgaa tgccccggga
     1501 aatcagatag tcgcgcaccg cctgcgcgcg tcttgcacca agggcgatgt tgtattcgcg
     1561 ggtgccgcgc tcgtcggcat ggccctcgat ggtgaattgg ctgtactggc tgtagtttgt
     1621 gagccactgc gcctgcttgt cgagcgtcgc gcgcgattgc ggcgtgagct cggacgagtc
     1681 ggtttcaaag aatacgcggt cgccgacatt gaccacgaaa tcctgctggc tgccgggtgt
     1741 tgcctggccg gcggcattgg cctgcgcgtc ctcggcattc ttggcacagg ccgatattgc
     1801 cagcgccgca accaggacgg cggcgaaacg agcgacgtgc agcgtcttcc tcatatcgat
     1861 cataatccgg tgcctccatt cacgcgtacc gaccggagct tgcgtgtggg tctagcaggt
     1921 cgttcgttaa acgaacgttc catcaacctt gatggaacct tgcgtggacc cattaaatct
     1981 accccaaccc cttaaaccgt ttctgatggt taagacacag tgaaaataag gccggttcac
     2041 gacattcagg acaaaagcgg tgaccaggcc ggatccgaag cgaagcttgg cgtcggaact
     2101 ttcagctcgt ttcgcccggt aatgtcgatg gtatagagcg aaggtccctg ccccacgtcg
     2161 cggaagaaca tcagcacgcg gccattgggc gcaaacgtcg ggccttcgtt gtggaagcct
     2221 tgcgtgagca cacgctcgcc cgacccgtcc ggcttgagaa tcccgatcga gaactgaccg
     2281 cgcgcttgcc cggtgaaggc aatgtagtcc ccgcgcggcg accagaccgg cgtcgaatga
     2341 tggccttcgc cgaagctgat gcgctgcgcc ggtccgccac tcgccgacat cacgtagatc
     2401 tgttgcgagc cgccgcgatc actttcgaag caaatgcgtg agccgtcggg cgaatagcac
     2461 ggagccgtgt cgatcgcagg cgtgtcggtg agacgcgtgg tctgtttcga gcgcaggtcc
     2521 attacaaaca ggttggcgtt gccgccctgc tgcaggctca tgatcacgcg ctggccgtca
     2581 ggcgaaaacc tgggcgcgaa cgtcatgccg gggaaattgc cgacaatctc gcgctggccg
     2641 gtttcaatat taagcagata aacgcgggga tcgccctgcc cataggacat gtaggtgatt
     2701 tcctgggtcg acgggttgaa ccggggcgtg agcacaagat cgtcaccgcg tgtaaggtag
     2761 cgaacggccg caccgtcctg atccatgatg gcgagccgct tcacgcggcg ctccttcgcg
     2821 ccggtctcgt cgacgaaaac gatccggctg tcgaaatagc ctttctcgcc ggtgagccgc
     2881 tcgtagatcg catccgaaat gatgtgcgcg atccggcgcc agttgtcggg cgtggtgaaa
     2941 tactgc
//
LOCUS       DASSRL010000381         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_972196, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000381 DASSRL010000000
VERSION     DASSRL010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972196"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFL49_07790"
     CDS             <1..645
                     /locus_tag="VFL49_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911917.1"
                     /translation="LPNAPKETLDDVRLSTAAHNSARFPVISPPGAVRNLQHDVVDRI
                     VDGGYIENYGALTAMELAAAIHAIEPELAPFVLVISNDPDENPDLNRIDVPDNVFLTD
                     VSIPLEAVENTRNGRGRLAVLELNAALDNLTSRSCERANTAHIRVWPQYAPVGAGDQK
                     KTRPVSLSWWLSTPIQIHLHQQTELGKSDNKNGSEIEKTWSALEDTSACMTARR"
     gene            complement(653..1621)
                     /locus_tag="VFL49_07795"
     CDS             complement(653..1621)
                     /locus_tag="VFL49_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7911918.1"
                     /translation="MGGRKIAALLVLLAGIGGAQAQDYPTKTVTVIVPFAAGGPADVT
                     GRIVADIFTRHLGQQFVVENVGGAGGTVGSLRAARAAADGYTLISGHMGTHAAAPFFY
                     PNLGYDPQKDFEPVGLIAEQPELLTVRKDFPANNLKEFIAYAKANESKLNMGHAGVGS
                     VSYVGCLLLNSAIGIKPTMVPFTGTAPVMNAILAGQIDYDCDPVLGPLPHVRAGNAKA
                     LAIASKKRSPLLPDVPTSHEQGLPEFDCAPFYAVFAPKGTPQAVIDKLADALNKGLSE
                     EAVQKRLGALGAEIAEPNRRGPKALAALVKSEVDRLTPILKAASSK"
     gene            2145..2354
                     /locus_tag="VFL49_07800"
     CDS             2145..2354
                     /locus_tag="VFL49_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HET7911919.1"
                     /translation="MTTPAHRRIACARCGASFDCGLSGECWCAAESYRLPMTRARVED
                     CLCPACLRKAATVLAGSRREAGQDG"
     gene            2424..>2601
                     /locus_tag="VFL49_07805"
     CDS             2424..>2601
                     /locus_tag="VFL49_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7911920.1"
                     /translation="MIDFYALTSPNVQKIFIMLEECGLPYSIKPVDVWAGEQFTPEFA
                     RINPLLKVPAIVDSD"
BASE COUNT          489 a          840 c          798 g          474 t
ORIGIN      
        1 ctcccgaacg ctcccaagga aacgctggat gacgtccgcc tgagcacggc ggcgcacaat
       61 tcggcacgct ttccggtcat ttcgccgccc ggcgcggtgc gcaatctcca gcacgatgtc
      121 gtcgaccgca tcgtcgacgg cggctacatc gaaaattacg gagcgctgac ggcgatggag
      181 cttgctgccg cgattcatgc aatcgagccg gaactcgcgc ctttcgtgct ggtgatatcc
      241 aacgatcccg acgaaaaccc cgatctcaac cgaattgacg ttcccgacaa tgtttttctc
      301 acggacgtat cgattccgct tgaagcggtt gaaaatacgc gcaatggccg cggccggctc
      361 gcagtgctgg agctcaacgc cgcgctcgac aacctgacca gccggtcttg cgagcgcgcg
      421 aatacggccc acatccgcgt ctggccgcaa tatgcgccgg tcggcgccgg tgatcagaaa
      481 aaaacgcgac cggtgtcatt gagctggtgg ctctccacgc cgattcaaat tcatctgcac
      541 cagcagaccg agctcggaaa gagcgacaac aaaaacggca gcgagatcga aaagacctgg
      601 agcgccctcg aggacacctc ggcctgcatg acggcgcggc gctaggatga tcctacttcg
      661 atgacgcagc cttcaggatc ggcgtcagac gatcgacttc gcttttgacc aatgccgcaa
      721 gcgctttcgg cccgcgccgg tttggctcgg cgatctcggc gccgagcgca ccgagacgct
      781 tctgcaccgc ttcctcgctc aagcccttgt tgagagcatc cgcaagcttg tcgatgaccg
      841 cctgcggcgt gcctttcggc gcgaacaccg catagaaagg cgcgcaatcg aactcgggca
      901 ggccctgctc gtgcgaggtc ggcacgtccg gcagcaacgg gctgcgcttc ttcgacgcta
      961 tggcgagcgc cttggcgttg cccgctcgca cgtgcggcaa cggcccgagc accggatcgc
     1021 agtcataatc gatctggccg gcgaggatcg cgttcatcac cggggcggtg ccggtgaacg
     1081 gcaccatggt cggcttgata ccgatcgccg agttgagcag caggcagccg acgtaggaca
     1141 ccgacccgac gcccgcgtgc cccatgttga gcttgctctc atttgccttc gcgtaggcga
     1201 tgaattcctt gagattgttg gcggggaagt ccttgcggac cgtcagcagc tccggctgct
     1261 cggcgatcag cccgaccggc tcgaagtcct tctgcggatc gtagccgagg ttgggataga
     1321 agaacggcgc cgccgcgtgc gtgcccatgt gcccggagat gagcgtatag ccgtcggcag
     1381 cggcgcgcgc ggcgcgcagc gatccgaccg tgccgccggc gccgccgaca ttctcgacta
     1441 cgaactgctg gccgagatgg cgcgtgaaga tgtcggccac gatccggccg gtgacgtcgg
     1501 ccggtccacc cgccgcgaac ggcacgatca ccgtcaccgt ttttgtcgga taatcctggg
     1561 cctgcgcccc gccgatgccc gcgagcagca caagcagcgc cgcaatcttt cgtcctccca
     1621 tcgtcccctc cccgtaagcc ttcaggtttt tatggatgga accataccat ggtgcgggag
     1681 cggatatagc cgtcgtcgtc gatctccgcc ggagcaaggt ggaactttct caccgacaaa
     1741 cctgtgcacg gttgcgcatc tctcatagag cggcgcgagc ggacacttat gatgacttgc
     1801 gggcacgtgg gaggcggcgt tgaaatcact ttgtccaatc gcggcagctc tgacactcat
     1861 tgtttcgggg ttggccgctt cggctgaaac atcggaagag cgacaggcct gcatcgggga
     1921 tgcattcagg gtttgctggt cggccattcc ggatcgcaac aatgtgcttc agtgcctgat
     1981 tgaaaacaga agtcggctta acccgagttg ccgcgaggta ataaccagat atacccgatc
     2041 acaccggcac agggtcacac gatccgcaca cagcggacgt tcgcgagccg agtgagctcg
     2101 gcgacgggct tgccttagct ttcggccgcg cgcaggctcg ttacatgacg accccggccc
     2161 accgccgcat cgcttgcgcc cgctgcggcg cttcatttga ttgcggtctc tccggcgaat
     2221 gctggtgcgc agccgagtca taccgtctgc ccatgaccag ggcgcgcgtc gaggattgcc
     2281 tttgccccgc atgcctgcgc aaggcggcga cggtgctcgc cgggagccgg cgcgaggcgg
     2341 gccaagacgg ctagccgcac ctgtatgcct tcgtctatag agagccgccg cagtccgcgg
     2401 ccgcaggacc agagacaatt ctcatgatcg atttttacgc gctgaccagc ccgaacgtgc
     2461 agaagatatt catcatgctc gaggagtgcg ggctgcccta cagcatcaag ccggtggacg
     2521 tgtgggccgg cgagcaattc acgccggaat tcgccaggat caatccgctc ctcaaagtgc
     2581 ccgcgatcgt cgacagcgac g
//
LOCUS       DASSRL010000382         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_552280, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000382 DASSRL010000000
VERSION     DASSRL010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552280"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFL49_07810"
     CDS             complement(<1..577)
                     /locus_tag="VFL49_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET7911921.1"
                     /translation="MQATTTVALSERPMLHALAKCWWALLLRGIAAIVFGVLAFAWPG
                     LTLVTLVLLYGAFALVDGVLSLIAAFTSGTKLVPTWWLIVVGLLGIAAGVVTFLWPGI
                     TAILLVVFIGAWALVHGIFEIIGAIQLRKEIDNEWMLIFGGLLSVLFGILVLIAPGAG
                     AIGLVWAIAAYSIVFGITFVALALRLRSHSHA"
     gene            complement(613..987)
                     /locus_tag="VFL49_07815"
     CDS             complement(613..987)
                     /locus_tag="VFL49_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911922.1"
                     /translation="MPALAFFAVVGSVLIALLFLADATLEKSDSPVIATSQRSGLPES
                     PHRADDVQILTTAPAPAPDMTSQAVLDAQPRPASQRPMKVHPEAEAARAEALPQHRRV
                     TQPMNDRYRPNSIFDRFSIKGQ"
     gene            complement(1693..2430)
                     /locus_tag="VFL49_07820"
     CDS             complement(1693..2430)
                     /locus_tag="VFL49_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7911923.1"
                     /translation="METKIAEIADGIYRLSTFVPDIAPPAGFTFNQFVVLGDEPLMFH
                     TGLRKMFPLNRDAVSRLIPPDRLRWIAFGHFEADECGAMNEWLAIAPNAQPAHGNTGC
                     LVSLNDFADRLPRVLEDGEVIDLGRGKRVRYIDTPHTPHGWDAGVIFEESTGTLLCGD
                     LFTQLGNGPALTEGDIIGPASAAEDLFRYSSLSPGMGTTIRALAKVSPQTLALMHGPS
                     FSGDGAGALHALADEYDRRIQHANSAN"
     gene            2519..3100
                     /locus_tag="VFL49_07825"
     CDS             2519..3100
                     /locus_tag="VFL49_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7911924.1"
                     /translation="MKLMARTYTLKRRAEQQAETRRRIVEAAVDLHGTVGPARTTFSM
                     VAERASVQRHTLYAHFPDELSLLMACSALALERDPLPDPAPWRRIEDRHERLRVGLTA
                     IYGWFERGADLAACILRDAEIHPPVRETVALRMGPSIAAYHETLGAKLNARQRAMLGL
                     ALSFYTWRSLVRDGGLTRGAAVGAMLQAIENAK"
     gene            complement(3127..>3540)
                     /locus_tag="VFL49_07830"
     CDS             complement(3127..>3540)
                     /locus_tag="VFL49_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911925.1"
                     /translation="RDGYVRVAVGCGDGYVSDKGVANLSWETTMRVLIAVLLLLACGS
                     AQAEIRINESRYVDGTLTISGETSPHRIVTLDGKYKTKSDGGGHFKFIEHYKPFTCMP
                     DIRSGQDVYSPVIAGCLISDDASTPVPGNTASNNN"
BASE COUNT          711 a         1061 c         1103 g          665 t
ORIGIN      
        1 cggcgtgact gtgactgcgc aggcgaagtg ccagcgcgac aaaggtgatg ccgaacacga
       61 tcgaataggc ggctatggcc cagaccagcc cgatcgctcc ggcgccgggc gcaatcagca
      121 ctaaaatacc gaacagtacc gacagtagcc cgccgaagat cagcatccac tcgttgtcaa
      181 tctccttgcg caactggatc gcgccaatga tctcgaagat gccgtgcacc agcgcccacg
      241 cgccgatgaa caccaccagc aggattgcgg tgatgccggg ccaaaggaac gttacgaccc
      301 cggccgcgat gccgaggagg ccgaccacga tcagccacca ggtcggaacc agtttggtgc
      361 cgctggtgaa ggcagcgatg agcgaaagca cgccgtcgac caaggcaaag gccccgtaaa
      421 gcaagaccaa agtcaccaaa gtcaggccgg gccacgcaaa cgcgagaacc ccgaagacaa
      481 tggcggcaat acctcgcaac aacagtgccc accagcactt ggcgagtgcg tgcagcatcg
      541 gacgctcgga taacgcgacg gttgttgttg cctgcatggc atttatctcc ttcgtgcaag
      601 catggttcgg ctttattgcc ccttgataga aaatctgtcg aatatcgagt tcggtcggta
      661 acggtcgttc atgggctgcg tgacgcgcct gtgctgcggc aacgcttccg cccgcgccgc
      721 ctccgcttcc ggatggacct tcattggccg ttgagaggcc ggcctgggtt gggcatcgag
      781 caccgcttgc gaggtcatgt ccggtgccgg tgccggcgcc gtggtgagaa tctgcacgtc
      841 gtccgcacga tgcggagact ccggtaagcc ggaacgttgg ctggtggcga tcacaggcga
      901 gtcgctcttc tccaatgtcg catcggcaag aaacagcaat gcgatcagca ccgaacctac
      961 aacggcaaag aaggcgagag ctggcatggc cgcgctccga ctttacgccc tgggtccagt
     1021 taagccggcc gacagcggtc aggtcgagtt ggaccatggg ccaacgaccg ctgcggcttt
     1081 aaaccccttt tccggcgcta tttcgcagct gccgtgggtc cggcgggccg atttcatagc
     1141 gagcgagccc gccctctcgc acgggccgta acgacccgga cttttgtttt tggccggtgg
     1201 cggacttttg aggaacgccg caagcgcgcg aagactgcgc tccaaggcat gatcggcata
     1261 aggatgcggc cattcggtcg ctcgcctgtc gtgatcgctt tcatgatttg tgtggccgcc
     1321 cgtttgacag gacccgtatc agacgcgtcc tgagcaaaca cggcggttga aaggatggcg
     1381 aacattgaca cggcgaccgc ggtaacaatc gcgaggcgaa gaatgctttg cggcaaccgc
     1441 gccgcgccac gtgccccgcc cgatccgctg ccgcgggccg gtgtgttgta tctggggcct
     1501 ggcgctccga cgacgaaggg aatctcgctc cgtgtaactc cgatgtcctt gagaatggcg
     1561 tcaggcaatt cattcagcgc ctgatgggtg ttgctggccg tcactgcgcg ggaaaccgaa
     1621 actcccaggc gagtggctga gccggaaacg tgaacaacag cgtcgatcat tgcaggctcc
     1681 atttactgtc gttcagtttg cggaattcgc gtgttggatg cgccggtcgt actcatcggc
     1741 gagtgcgtgc aaagcgcccg caccgtcacc cgagaacgat gggccgtgca tcaaggccag
     1801 cgtttgcggc gagacttttg cgagtgcgcg tattgtcgtg cccatcccgg gactgaggct
     1861 ggaataccgg aacaggtctt cggcagcgct cgccggcccg atgatgtcgc cttcggtcag
     1921 tgccgggcca ttcccgagct gggtgaacag gtcgccgcac agcagcgtcc ccgtcgactc
     1981 ttcaaagatc acacccgcgt cccagccgtg cggggtatgc ggcgtgtcaa tgtagcggac
     2041 tcgcttgccg cgacccaggt cgatgacctc tccgtcctcg agcacgcgcg gtagccggtc
     2101 ggcaaaatcg ttgagcgaga ccaggcagcc ggtgttgccg tgcgcaggtt gtgcgttcgg
     2161 ggcaatcgca agccactcat tcatcgcgcc gcactcgtcg gcctcgaaat ggccgaaagc
     2221 gatccagcgc agacggtcgg gcggaatgag gcggcttaca gcgtcgcgat tgagcggaaa
     2281 catcttccgc agcccggtgt ggaacatgag cggttcgtcg cccaggacaa cgaactgatt
     2341 gaaggtgaag ccggcgggcg gggcgatatc cggcacaaac gtcgaaagcc gataaatgcc
     2401 gtcggcgatt tcggcgattt tggtctccat ggcgggctcc taagtactga atttgaaggt
     2461 acatacatgt gtactgaaaa ttcagacctt gtcaagaatg actacacatg atagtgcaat
     2521 gaaattgatg gcacggacct ataccctcaa gcgccgggct gagcagcagg cggaaacgcg
     2581 gcgccgcatc gtcgaggcgg ccgttgatct gcacggcacc gtgggccccg cgcgcacgac
     2641 tttcagcatg gtggctgaac gggcgagcgt tcagcgtcat acgctctatg cgcattttcc
     2701 cgacgaactg agccttctga tggcctgctc ggcgctggcg ttggaacgcg atccgctgcc
     2761 ggaccccgcg ccttggcggc gtatcgaaga tcggcacgaa cggctgcgcg tgggtctcac
     2821 cgccatctat ggctggtttg agcgcggcgc tgatctcgct gcgtgcatct tgcgcgatgc
     2881 cgaaatacat ccgccggtga gagaaactgt ggcgttgcgg atggggccga gcatcgcggc
     2941 ctatcacgag acgctcggcg cgaagctcaa tgccaggcaa cgggcgatgc tgggcttggc
     3001 cctgagcttc tatacctggc gaagcctggt ccgcgatggc gggctcacgc gcggcgccgc
     3061 cgtcggtgcg atgctgcaag ccatcgaaaa cgcaaaatag aaattacatc ccggcgtcgc
     3121 cgctcgctaa ttattgttgg aggctgtatt tccaggcacc ggcgtgctgg cgtcatcgga
     3181 aatcagacag ccggcaatga cgggtgaata gacgtcttgg ccggagcgga tgtcgggcat
     3241 acacgtgaac ggcttgtaat gctcgatgaa cttgaaatgg ccgccaccgt cactttttgt
     3301 tttatacttg ccgtcgagcg tcactatacg gtgtgggctg gtctctcctg agatggtcag
     3361 cgtgccatcc acgtaacgcg attcgttgat ccggatctcc gcctgcgcag aaccgcaggc
     3421 taacagcagc aagactgcaa tgagcacgcg cattgtcgtt tcccaggata agttggcgac
     3481 acccttatcc gatacataac catcgccgca cccgactgca acgcgcacgt acccatctcg
//
LOCUS       DASSRL010000383         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1619924, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000383 DASSRL010000000
VERSION     DASSRL010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619924"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="VFL49_07835"
     CDS             <1..1838
                     /locus_tag="VFL49_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HET7911926.1"
                     /translation="ISGSSVANVVTTGTFTIPLMKRVGYSPVQAGAIECAAGVNGQLM
                     PPVMGAAAFLMAEYVGISYSDVVRHAFLPAIITYGALFYIVDIEAAKKGMKGLPRERR
                     RSRLQSLIYSLMTVSGFVILSGLIYYGFGWTKTVLGEAATPLAGALLVALYIALIRLR
                     ARHPDLPLDDPRSPLIKVPDFYEVAVTGLHYFVPVFVLIWCLMIEEMSPGLAAFWGVV
                     YMGGLVLTQRPLMTFFRGEHSYTREWRTGIEEFIAGLEAGGRNMAGVGIATATAGIVV
                     GTVTLTGVGLVMTEIVEFLSAGNFVMMLVLVALICLVLGAGLPTTASYVVVATLMAPV
                     VVELAAENDIAVPLISVHMFVFYFGLMADVSPPVGLAAYAAGALAGADPMKVGWQSMR
                     YENRTALLPFVFIFNPEILIIDIGGWLHFGLVVLCGVLAMGCFVAASQNWLLTRNRWY
                     ETLLLLLICFTLFRPGYWLNNFMPPFEDKPAAEVTRVAGELKRGSTIRIRLASQSRLG
                     EDVDKVIRLTMRTDGSGADRLKRAGLTLSTLGDKVTVQSVAFGSEAAKYGLSQGDTVL
                     AVLVPTKRPDPFWFTIPAFILLGLIVLLQRRRKQKALVAAGVGSV"
     gene            2149..>2450
                     /locus_tag="VFL49_07840"
     CDS             2149..>2450
                     /locus_tag="VFL49_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET7911927.1"
                     /translation="MTIRRSYYVRLFGMDIADSAWFSLKVHFDFTHPRGIHIGPESYV
                     AFGAVILTHDMTRGPYADTRIGARCFIGAHSIILPGLTVGDGSIVGAGSVVTEDVPP"
BASE COUNT          417 a          717 c          778 g          538 t
ORIGIN      
        1 tgatttccgg ctcgtcagtg gcgaatgtcg tgacgaccgg taccttcacg attccgctga
       61 tgaagcgcgt cggctattct ccggtgcagg ccggtgcgat cgaatgcgcg gctggcgtaa
      121 acggccagct gatgccgccg gtgatgggtg ccgcggcgtt tctgatggcc gaatatgtcg
      181 gcatcagcta ttccgacgtc gtgcgccacg cgttcttgcc ggcgatcatc acttacggcg
      241 cgctgttcta catcgtcgat atcgaagcgg cgaagaaagg catgaaaggc ctcccgcgcg
      301 aacggcggcg ctcgcgcctg caaagcctga tctattcgct gatgacggtc agcggcttcg
      361 tcattctttc cgggctcatc tattacggat ttggctggac gaagacggta ctcggcgaag
      421 cggcgacacc gctcgccggc gccctcctgg ttgcgctcta cattgcgctc attcgcctgc
      481 gcgcgaggca tcctgatctg ccgctcgatg atccgcgcag cccgcttatc aaggtgccgg
      541 acttctacga ggtcgcggtg accggcctgc attatttcgt gccagtcttc gtgctgatct
      601 ggtgcctgat gatcgaagaa atgtcgccgg ggctcgccgc cttttggggc gtcgtctaca
      661 tggggggcct cgtgctgacg cagcggccgc tgatgacgtt tttccgcggc gagcacagct
      721 atacgcggga atggcgcacc ggcatcgagg agttcatcgc cggactggag gcgggcggtc
      781 gcaacatggc gggcgtcggc atcgcgaccg cgaccgcagg tattgtcgtc ggaacggtga
      841 cgctgaccgg cgtcggcctc gtgatgacgg agatcgtcga gtttctctcc gccggcaatt
      901 tcgtgatgat gctggtgctc gtggctctaa tttgcctcgt actcggcgcg ggcctgccga
      961 cgacggcgag ttatgtcgtc gtcgcgacgc tgatggcgcc ggtggttgtc gagcttgccg
     1021 cagaaaacga tatcgccgtg ccgctcatct cggtgcatat gttcgtcttc tattttggat
     1081 tgatggccga cgtgtctccg ccggtagggt tagctgctta tgccgcgggc gctcttgccg
     1141 gcgccgatcc gatgaaggtc ggctggcaga gcatgcgcta cgaaaaccgc acggcgcttt
     1201 tgccgttcgt gttcatcttc aatcccgaaa ttctgataat cgacatcggc ggctggttgc
     1261 actttgggct cgtcgtgctt tgcggcgtcc tcgcgatggg ctgcttcgtc gcggcaagcc
     1321 agaactggct gctgacgcgc aatcgctggt acgagacact gctcctattg ctgatctgct
     1381 tcacgttgtt ccggccaggc tattggctga acaacttcat gccgccgttc gaggacaagc
     1441 ccgcggccga ggtcacgcgc gtcgccggcg agctgaagcg cggatcgacg atccggatac
     1501 ggctcgcgag ccaatcgcgg ctcggcgagg acgtcgacaa agtcatccgg ctcactatgc
     1561 gtaccgacgg aagcggcgca gatcgcttga agcgtgcggg gctaacgctt tcgacactcg
     1621 gcgacaaagt cacggttcaa tccgtcgcct tcggaagcga ggcggcgaaa tatggtctct
     1681 cgcagggaga cacggttctt gccgttctgg tcccgacgaa acgccccgat ccgttctggt
     1741 tcacaattcc ggctttcatt ttattggggc tgatcgtcct attgcagcgg cggcgaaagc
     1801 agaaggcgct tgttgccgct ggcgtaggtt cggtctgaca aaacaggaat cggaaccgcg
     1861 gaagattcga atctcccaac tgtggttccg gaagctgccg cttgtgcgcc aaacctacgc
     1921 caaatttccc tgaaatagat aattggactc tgttgccata gcgacggcag tctgaacggg
     1981 ggcatcttga gcaagctgcc agctgacacc gggcgtgatg cagtagtgcc cccgagcatt
     2041 gtgagactag gggttgaaat cgcagcacgc ggaaatcgcg gggtcgctgt gcgtaatcct
     2101 aaacgcggga ttttgcgcgc cctgaagcta gcgctgcggc aggggctgat gacgatacgg
     2161 cgcagttatt atgtgcgcct attcggcatg gacatcgccg atagcgcctg gttctcgctc
     2221 aaagtgcatt ttgacttcac gcatccccgc ggcatccaca tcggcccgga gagctatgtc
     2281 gccttcggcg cggtaatcct cactcatgac atgacgcgcg gcccttatgc ggacacgcgc
     2341 atcggcgcgc ggtgcttcat cggcgcgcac agcatcattc tgccgggtct cacggtcggc
     2401 gacggttcga tcgttggggc tggcagcgtt gtaacggagg atgttccgcc
//
LOCUS       DASSRL010000384         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1077451, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000384 DASSRL010000000
VERSION     DASSRL010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077451"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFL49_07845"
     CDS             <1..757
                     /locus_tag="VFL49_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HET7911928.1"
                     /translation="LQRYGTALSQARESMTPAMATRLVGKPLYFGGMMVRQGDADAMV
                     AGCANPTRRVIEAGLMTCGLAEGIALPSSYFLIVVPDFLGKGPKPFVFADCAINADPD
                     AGELADIAVASARSAEALLGEKPRVAMLSFSTKGSAQHVRIDKVREALTLVRQRHPDL
                     AIDGEFQADAALVPGVAEKKVKEPSAVAGQANVLIFPDLDSGNIAYKLTQWLGGAQAI
                     GPFLQGFAKPISDLSRGASVDDIVTTCAVVAAS"
     gene            complement(865..2454)
                     /locus_tag="VFL49_07850"
     CDS             complement(865..2454)
                     /locus_tag="VFL49_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7911929.1"
                     /translation="MADFDGVVIGAGHNGLTLAAYLSRAGLRVAVLERNSRIGGGSST
                     EERILPGFKLNLHANFFMGLGLCPLLHDLELHRFGFSYIEPPVQQAAVFRDGTGIVVY
                     KDLDKTCASLARFSRQDAETFRTLWHTYCEEMRPLLVSLLYNAPMPREQLVDRLSGPK
                     AKELLSHAQHDLFSVVRKHFVDERIRTLFTSYMHVITTENVPGAGIVFPAIFANIASF
                     TLPTGGAMSLPLALARVVEAGGGEIVTGADVKDIKVAGGRATGVTLADGRSINGRKFV
                     ASAIDFPATVQMAGEELFPEPVREKAKGWHWGNHSLVTLHLALKDPPAYRAAAFDPDV
                     SRAYNIFFGMDRIEDVASCFDDCAAKKFPRVLMGNGACNSQFDSSYAPAGRHIAFWWP
                     FAPYSVDGSPQEWERNREGYAQKVLDYWRIYASNLEGDNLLGSYLFTPLDVETLNANM
                     RQGAVRVGAYVPNQLGINRPHPLMPGSRTPVEGLYLCGSSSGNGGGINGAAGYIAANA
                     IADDLKLKRAWKPVPAPEWKH"
     gene            complement(2466..3044)
                     /locus_tag="VFL49_07855"
     CDS             complement(2466..3044)
                     /locus_tag="VFL49_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7911930.1"
                     /translation="MAGKGSITAHNAKVMNRFELTKQLVGQLKREEAVIGGIGNANFD
                     LWASGQRPQNFYMLGSMGLAIPIALEGDGSLLMQLGCLATVAERAPKNLTIVVWDNGI
                     YQITGSQPTPGAASADLVAIARGAGIVKSAWAGDEADFDNLIAGALKDGGPNLIAARI
                     DGKPAVGTTDRDPVLIRERFMRGLGVRSSLPD"
     gene            complement(3047..3583)
                     /locus_tag="VFL49_07860"
     CDS             complement(3047..3583)
                     /locus_tag="VFL49_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HET7911931.1"
                     /translation="MTDTTTQATAWHDIVRASLKANDVRLVVYVPDRVFTPLIEALHA
                     DTFFTTFAATREEEALGIITGAWMGGVRGAVLMQTSGFGTIPNALASLVVPSQIPALI
                     FVSERGTLGEFNLGQALVCKTMRPVLNSLAVENVTISRRDELAFILDRSIKQAVATQA
                     PVTFILSPLLTGGKVFAG"
     gene            3740..>4726
                     /locus_tag="VFL49_07865"
     CDS             3740..>4726
                     /locus_tag="VFL49_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7911932.1"
                     /translation="MSKFNSHFTPSRRSIIKGALAAGVAAPAFLRGRAAYAAYPERPV
                     KVVVANTPGGPSDLVARMITAALQQSTGKTFFVENIGGAGGNIGMGAAARAEGDGYTI
                     LLATNAYSVNASLYKKIPYDPLKDFVGVSELATSPNTFVVRSELPAKTIKEFVALARA
                     NPEKYNCATPPIGTTPQIQLEVLKLREKLPKLEDVVFKGGGDALQALLSSTVQLSSGS
                     LPPAAPHIKSGKLRCLTVTGETRWPDMPDVPTMLESGYKDFVFATDTVLLAPSKTPPE
                     AIKWIETETLKVLGTAEMKEKLFKAGFQVRAKGAKDAWARVTKEIDMFKSIID"
BASE COUNT          914 a         1452 c         1502 g          858 t
ORIGIN      
        1 cctgcaacgt tacggcacgg cgctttcgca ggcgcgcgaa tcgatgacgc cggcaatggc
       61 aacgcgcctt gtcggcaagc cgctttattt cggcggcatg atggtgaggc agggcgatgc
      121 cgacgccatg gtcgccggct gtgccaaccc gacccggcgc gtgatcgagg ctggcctgat
      181 gacatgcggc ctcgctgagg gaatcgcgct gccgtcgagc tatttcctga ttgtcgtccc
      241 ggattttctc ggcaagggcc cgaagccgtt cgttttcgcc gattgcgcga tcaatgccga
      301 tcctgacgcc ggcgagcttg ccgatatcgc ggttgcgtcg gcgcgcagcg cggaagcgct
      361 tctcggcgaa aagccgcgcg tcgcgatgct ctccttctcg accaaaggca gcgcgcagca
      421 tgtgcgaatc gacaaagtgc gcgaagcact caccctcgtc cggcagcgtc acccggacct
      481 cgcgatcgac ggcgaattcc aggcggacgc ggccctggtg ccgggtgttg ccgaaaagaa
      541 ggtgaaggag ccgagcgcgg tcgccgggca ggccaatgtg ctgatcttcc ccgatctcga
      601 ctcaggcaat atcgcctaca agctcacaca gtggcttggc ggcgcgcagg caatcgggcc
      661 gtttctgcag ggttttgcaa aaccgatcag cgacctgtcg cgaggcgcga gcgtcgatga
      721 catcgtcacg acctgcgccg tggtggctgc gagctagagc cgaatccttt caagttgact
      781 ggtcatgccc gcccttgccg gaataaaagc ccggccatga cggcggatat ttcaacagga
      841 aaggcgattt ctgattctgt gtgctcaatg cttccattcc ggcgcgggaa ccggtttcca
      901 cgcgcgctta agcttcaaat cgtcggcgat ggcattcgcc gcaatgtagc cggcggcgcc
      961 attgatgccg ccgccgttgc ccgacgacga cccgcagaga tagaggcctt ccaccggcgt
     1021 gcgcgatccc ggcatcagcg gatgcggccg gttgatgccg agttgattcg gaacataggc
     1081 gccgacgcgc accgcgccct ggcgcatgtt ggcgttgagc gtctccacat cgagcggcgt
     1141 gaacagatag ctgccgagca gattgtcgcc ttcgaggttc gaggcgtaga tgcgccagta
     1201 gtcgagaacc ttctgcgcgt agccctcgcg gttgcgctcc cattcctgtg gcgagccgtc
     1261 gaccgaatag ggcgcgaagg gccaccagaa tgcgatgtgt ctgcccgccg gtgcgtagct
     1321 cgaatcgaac tggctgttgc aggcaccgtt gcccatcaaa acgcggggaa attttttcgc
     1381 ggcgcaatcg tcgaagcagc ttgcgacatc ctcgatgcgg tccataccga agaagatgtt
     1441 gtaggcgcgc gacacgtcgg gatcgaaggc ggctgcgcgg taggccggcg gatccttgag
     1501 cgccaggtgc agcgtgacga ggctgtggtt tccccagtgc cagcctttcg ccttctcgcg
     1561 caccggctcc gggaacagtt cctcgccggc catctgaacg gtggccggaa aatcgatcgc
     1621 gctcgccacg aacttgcggc cattgatgct gcgaccgtcg gcgagcgtga cgccggtggc
     1681 acggccgccc gcgaccttga tgtccttcac gtcggcaccg gtgacgattt ccccgccgcc
     1741 tgcctcgacg acacgcgcga gcgcaagcgg cagcgacatc gcgccaccgg ttggaagcgt
     1801 gaagctcgcg atgttggcaa agatggcggg aaagacgatg ccggcgccgg gcacgttctc
     1861 ggtggtgatc acgtgcatat aggacgtgaa cagcgtgcga atgcgctcgt cgacgaaatg
     1921 cttgcgcacg acgctgaaga gatcgtgctg ggcgtgtgag agcagttctt ttgccttggg
     1981 gccgctcaga cggtcgacga gctgctcgcg cggcattggc gcgttgtaga gcagcgacac
     2041 cagcagcggc cgcatctcct cgcaataggt gtgccagagg gtccggaacg tctccgcatc
     2101 ctgcctggaa aagcgggcca gcgacgcgca agttttgtcg agatccttgt aaacgacgat
     2161 gccggtgcca tcgcggaaga ccgccgcctg ctgcaccggc ggctcgatgt aggaaaagcc
     2221 gaagcgatga agctcgagat catgcagcag cgggcagagc cccaggccca tgaagaaatt
     2281 ggcatgcagg ttgagcttga agcccgggag gatgcgctct tccgtgctgg agccgccgcc
     2341 gatccggctg ttgcgctcga gcacggcgac gcggagaccg gcacgcgaaa gatatgcggc
     2401 aagcgtcagg ccgttatggc cggctccgat aacgacgccg tcgaaatccg ccattgtgcc
     2461 cccggctagt ccggcaaact gcttcgcact cccaatccac gcatgaaccg ctcgcggatc
     2521 agcaccggat cgcggtcggt ggttccaacc gcgggtttcc cgtcgatccg cgccgcgatc
     2581 agattcggcc cgccgtcttt aagggcaccc gcaatcagat tgtcgaaatc ggcctcgtcg
     2641 ccggcccagg cgcttttgac gatgccggcc ccgcgcgcga tcgcgaccag atcggccgaa
     2701 gcagcacccg gcgtcggctg cgagccggta atttgataga tgccgttatc ccagacgacg
     2761 atggtcagat ttttcggcgc gcgctcggcg acagtcgcaa ggcagcccag ctgcatcagc
     2821 agcgagccgt cgccttcgag cgcgatcggg atcgcaagac ccatgctgcc gagcatgtag
     2881 aaattttgcg ggcgctggcc gctggcccaa agatcgaaat tggcgttgcc gatgccgccg
     2941 atgacggctt cctcgcgctt gagttgcccg acgagctgct tggtcagttc gaaacggttc
     3001 atcactttcg cattgtgcgc ggtgatgctt cctttgccgg ccatggtcac cccgcgaaca
     3061 ctttgccgcc ggtgagcagc ggcgagagga tgaaggtgac cggcgcctgc gtcgccacgg
     3121 cctgcttgat cgagcgatcg aggataaacg ccagctcgtc ccggcgcgag atcgtgacgt
     3181 tctcgacggc aagtgaattg agcaccggcc gcatggtctt gcagaccagc gcctgcccga
     3241 gattgaactc acccagggtt ccgcgctcgg aaacaaatat gagcgccgga atttgtgacg
     3301 gcaccaccag cgacgccagc gcattcggga tcgtgccgaa gccgctggtc tgcatgagga
     3361 cggcgccgcg cacgccgccc atccaggctc ccgtgatgat gccgagcgct tcctcttcgc
     3421 gcgttgcggc aaaggtcgtg aagaacgtgt cggcgtggag cgcctcgatc agcggcgtga
     3481 acacccggtc cggaacatag acgacgagtc tgacgtcatt ggccttcagg cttgcgcgca
     3541 cgatgtcatg ccaggccgtt gcttgggttg tcgtatctgt catgccatga gcggctgcgt
     3601 cgttgccgtt tgatatcgtg aaaatcgtgt cgtcgccgcc gcgctgatgc gaggccattg
     3661 ctccaatatt cgcaaaagct tagcctaaga taattcacgg cgctaaccag aaaacacggc
     3721 ggaagatcag gggaggacga tgagcaaatt caattcacac tttacgccat cacggcgcag
     3781 catcatcaag ggcgcgttgg cggcgggcgt tgctgctccg gcatttctgc gcgggcgcgc
     3841 ggcctatgcc gcctatcccg agcggccggt gaaagtggtg gtcgccaaca ctcccggcgg
     3901 cccttcggac ctcgtggcgc gcatgatcac cgcggcgctt cagcaatcga ccggcaagac
     3961 ctttttcgtc gaaaatatcg gcggagccgg tggcaatatc gggatgggtg ccgccgcgcg
     4021 cgccgaaggc gacggttaca cgatcctgct tgcaaccaat gcttactcgg tcaacgcaag
     4081 cctctacaaa aagattccct atgacccgct caaggatttc gtgggcgtga gcgagcttgc
     4141 gacgtcaccc aacacattcg tcgtgagatc cgagcttccc gccaagacga tcaaggaatt
     4201 cgtcgcattg gcgagagcca atccagagaa atacaactgc gccacgccgc cgatcggaac
     4261 gacaccgcag atccagctgg aagtgctgaa actgcgggaa aagctgccga agcttgaaga
     4321 tgtcgtattc aagggcggcg gcgacgcgtt gcaggcgctg ctgagcagca cggttcagct
     4381 aagctccggc tcgctgccgc cggcggctcc gcacataaaa tccggaaagc ttcgctgcct
     4441 gacagtcacc ggtgaaaccc gctggccgga tatgcccgat gttccgacca tgctggaatc
     4501 cggctacaag gatttcgtgt ttgccaccga cacggtgctg cttgcgccgt ccaagacgcc
     4561 gccggaagcg atcaagtgga tcgaaaccga aacgctcaag gtgctcggca ctgcggagat
     4621 gaaggaaaaa ttgttcaaag ccggcttcca ggtgcgggca aaaggcgcca aggacgcctg
     4681 ggcgcgggtg accaaagaaa tcgatatgtt caaaagcatc atcgat
//
LOCUS       DASSRL010000385         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_902819, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000385 DASSRL010000000
VERSION     DASSRL010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902819"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFL49_07870"
     CDS             complement(<1..690)
                     /locus_tag="VFL49_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911933.1"
                     /translation="MRRRDFSKLALAGAVGTVAAPAVVHAQTTFNWKMTSFYGPNAAF
                     YSTGPGSAKDLVKRIEEMSNGRMKIQFFGAGELIPAAEGFDAVSTGIVEMNYANAYFW
                     TGKVFAGQYFTAVPFGLNFQGLNGWMYDGGGLQLWREVYDRFNLVPFLCGNTGVQMTG
                     WFKKPIEKLSDFNGLKMRIPGLAGRVYQSIGVDVRLLPAGEIFPALERGVIDAAEFVG
                     PYLDRQLGLQRA"
     gene            complement(881..1309)
                     /locus_tag="VFL49_07875"
     CDS             complement(881..1309)
                     /locus_tag="VFL49_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911934.1"
                     /translation="MKRNASMCGVFAAVLMSFICAATVMAQPFPFDQELLLDAEPMRP
                     GKRMPILIVEANGDARIDLWCRTVSARVEIADTSMKIEVGQLPEDLPAMQGAGQCTPE
                     RIKADEEMLASIVQVTEWRREGEAILLIGPATMKFRPATN"
     gene            complement(1309..2646)
                     /locus_tag="VFL49_07880"
     CDS             complement(1309..2646)
                     /locus_tag="VFL49_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7911935.1"
                     /translation="MADENLTALSASKAAAEIARGAVSSEDYTRACLDQIAALEPKVQ
                     AFAHLDAEHALAQARERDEWRKSGRPIGPLHGVPVGIKDIIDTSDYPTECGSPALSGR
                     RPREDATVVSRLRAAGAVIIGKTVTTEFAYFNPGKTRNPHDPERTPGGSSSGSAAAVA
                     AQMIPLAMGSQTNGSIIRPGAFCGVFAMKPTHGLVPRTGVLPLSRTLDHIGPFARSVD
                     DLALALDVIAGFDPDDPDTRPVATGGYSKIASEDFPLPPRFAFVPTPVWNKADAQTHE
                     AFEGLAEELGEACFTFDLPERYAAAWDAQRVIMATEMAHNLGKLVDTGGELISKTTRD
                     LIEDGRRTTAVQYLAAGAGAEPLRQGLNQLFQQECTAIITPAAPGVAPKGTATGNPAF
                     CTLWTYVGLPSITIPLMTGEGGMPLGVQLVGPSGDDARLLRTARALIKQLSEM"
     gene            complement(2663..>3225)
                     /locus_tag="VFL49_07885"
     CDS             complement(2663..>3225)
                     /locus_tag="VFL49_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7911936.1"
                     /translation="VGAAGAFLLALQAKTLDWKRTKEAVFLTAKTTAMVCWLFVGSAL
                     FSGVFAILGGQALVEQWVLSLNMTPIQFMILSQAIIFILGWPLEWTEIIVIFVPIFLP
                     LLKHFNIDPVLWGVLVFVNLQAAFLSPPVAMSAFYLKGVAPKHVTLNQIFNGMMPYML
                     IVILCMIVMYLWPGMTLWLPNYLYGN"
BASE COUNT          673 a         1000 c          958 g          594 t
ORIGIN      
        1 cgcgcgctga aggccgagct gccggtcgag gtaaggtccg acgaactcgg ctgcatcgat
       61 cacgccacgc tcaagcgccg gaaaaatctc gccggcgggc aacaggcgta catcgacgcc
      121 gatcgactga taaacacgcc cggcaagacc ggggatgcgc attttcagac cgttgaagtc
      181 ggagagtttt tcgatcggtt tcttgaacca accggtcatc tgcacgccgg tattgccgca
      241 gagaaacggt acaaggttga agcgatcgta gacttcacgc cacagctgga ggcctccgcc
      301 gtcatacatc cagccattga ggccctggaa gttgagaccg aagggaacag ccgtgaaata
      361 ctgtcccgca aaaactttgc ccgtccagaa ataggcgttg gcatagttca tctcgacgat
      421 gccggtggac accgcgtcga agccttcagc cgccggtatc aattcgcccg cgccgaaaaa
      481 ctgaatcttc atacgtccgt tggacatttc ctcgatgcgc ttgacgagat ccttcgcgct
      541 gccgggtccg gtcgaataga acgccgcatt cggaccgtaa aagctcgtca tcttccaatt
      601 gaacgtggtc tgagcatgga ccacagccgg cgcggcaaca gtgccgacag caccagcgag
      661 cgctaacttg ctgaaatcac gacgacgcat gacacccctc cccctgatac aagtccgtcg
      721 agcaccaacg tttgcgtgaa aggccctcca aatatcgcgt caaaccagcc gcagcttcag
      781 acagcatcct atgcccgctt cggacgcgtg ggcaatcgga agttcggggt tcagtcggga
      841 atttcttcgc tggcctacca gggccaggcc cgcaagctag ctagttggtc gccggccgga
      901 atttcatcgt tgccggccct atcagaagga ttgcttcgcc ctcgcgccgc cactccgtca
      961 cctgaacgat cgatgcaagc atctcctcat ccgccttgat gcgttcaggc gtgcattgac
     1021 cggcgccttg catcgcgggc agatcttccg gcaactggcc cacctcgatt ttcattgacg
     1081 tgtcggcaat ttccacgcga gccgaaacgg ttctgcacca gaggtcgatc cgcgcatcgc
     1141 cattcgcttc gacgatcagg atcggcattc gcttgcccgg ccgcatcggt tcggcatcaa
     1201 gcaaaagctc ctggtcgaag gggaacggct gcgccatgac cgtcgctgca cagatgaacg
     1261 acataagaac ggcggcgaag acgccgcaca tcgaagcgtt gcgtttcatt acatttcgga
     1321 aagctgtttt atgagcgccc gcgccgtccg caacagccgg gcatcgtctc ctgatggtcc
     1381 gaccagttgc acaccgagcg gcatcccacc ctcgcccgtc atcagcggaa ttgtgatcga
     1441 cggcagaccc acgtaggtcc aaagcgtgca aaacgccgga ttgccggttg ccgtaccctt
     1501 cggagcaacg ccgggcgccg ccggcgtgat gatcgcagtg cattcctgct ggaataactg
     1561 gttcagtccc tgccgcaagg gctctgcccc ggcgccggcc gcaaggtatt gcaccgccgt
     1621 ggtccggcgg ccgtcctcga tgagatcgcg ggttgtcttg ctgatgagct cgccgccggt
     1681 gtcgaccagc tttccgagat tatgcgccat ttccgtagcc atgatcacac gctgggcatc
     1741 ccaggcggcg gcgtagcgct cgggcaaatc gaatgtgaag caggcttcgc ccagctcctc
     1801 ggccaatccc tcgaatgcct catgcgtctg cgcgtcggct ttgttccaga ccggcgtcgg
     1861 tacgaatgcg aaacgcggcg gaagcgggaa atcttcggac gcaatcttgc tgtagccgcc
     1921 ggtcgcgaca gggcgtgtgt ccggatcgtc ggggtcaaag ccggcgatga cgtccagagc
     1981 caaagcaaga tcatcgaccg accgcgcgaa cggaccgata tgatcgaggg tgcgcgacag
     2041 cggcaagacg ccggttcgcg gcaccagacc gtgggtcggc ttcatggcaa acacgccgca
     2101 gaacgcgccc ggcctgatga tcgatccgtt ggtttggctg cccatcgcaa gcggaatcat
     2161 ctgcgccgcg acggcggccg ccgatcccga tgacgaacct cccggcgtac gctcgggatc
     2221 atgcggattg cgcgttttgc cgggattgaa ataggcgaac tcggtcgtca cggttttacc
     2281 gatgatgacc gcgccggcag cccgcaggcg ggagacgacg gtcgcatcct cgcgcggccg
     2341 ccggccggac agagccggcg atccgcattc cgtcggatag tcggaggtat cgatgatgtc
     2401 cttgatgccg accggaacgc cgtgcagcgg accgatcggc cgtccgctct tgcgccattc
     2461 gtcgcgttcg cgcgcctgcg ccagcgcgtg ctctgcatcg aggtgagcga aggcctgaac
     2521 ttttggctca agcgccgcga tttggtcgag gcaggccctc gtgtagtctt cggacgatac
     2581 cgcaccacgc gcgatttccg cggcggcttt gctcgcggaa agagctgtca gattttcatc
     2641 agccaattcg gcaggctccg ccttagttac cgtagagata gttcggaagc cagagggtca
     2701 tgcccggcca gagatacatg actatcatgc acaggatcac gatcaacatg taaggcatca
     2761 tgccgttgaa aatctggttg agcgtgacgt gcttgggtgc gacccccttg aggtagaacg
     2821 ccgacatggc cactggcgga gacaggaagg ccgcctggag attcacgaag accagcacgc
     2881 cccacagaac gggatcgatg ttgaagtgct tgagcagcgg cagaaagatc ggaacgaaga
     2941 tgacgattat ctcggtccat tccagcggcc agccaaggat aaaaatgatc gcctgcgaca
     3001 ggatcatgaa ctggatcggc gtcatgttga gcgagagcac ccactgctca acaagcgcct
     3061 gaccgccaag aatggcgaaa acaccggaga acagtgccga gccgacgaac agccagcaca
     3121 ccatcgcggt cgtcttcgcg gtcaggaata cggcttcttt cgtccgcttc cagtcaaggg
     3181 tcttggcttg cagggccaaa aggaaggcac ccgctgcgcc gacac
//
LOCUS       DASSRL010000386         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_955146, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000386 DASSRL010000000
VERSION     DASSRL010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955146"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VFL49_07890"
     CDS             complement(<1..1811)
                     /locus_tag="VFL49_07890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7911937.1"
                     /translation="MTMRVVDILVPVALDRTYSYRVPAGLELAPGNLVSVSLGARECM
                     GVVWAENSAPNPRLHNRLKDVDEKLDVPPLKAELRQFVDWVSGYTLASRGTVLRMALR
                     MGEHLGPGREQVGVRFVGQPPQRITPARARVLRLVADGFARPKCSAAKEAGVSASVIN
                     GLIDEGTLETVVIPPDPVALPSDPDFRKPEFSLAQLAAADGLRKTVEQGGYTVTLLDG
                     VTGSGKTEVYFEAVAENIRHKRQTLILMPEIALTAQFLDRFTGRFGTRPAEWHSQISQ
                     RKRAQTWAAVAANEVSVIVGARSALFLPYADLGLIVVDEEHDPAYKQEDGAHYHARDM
                     AVVRGSIAKIPVVLASATPSVESEVNARRGRYRHVHLPERFGGAHLPSIEAIDMRREG
                     PPRGRFISARLAEEVAVSLTRGQQALLFLNRRGYSPLTLCRTCGFRFQCPNCDAWLVD
                     HRFKRRLVCHHCGYSIPPPAECPNCHAAESLAAVGPGVERLAHEAAELFPQARILVLS
                     SDLVESIDRLRQELDDVAEGRFDLVIGTQLVAKGHHFPKLNLVGIVDADLGLGNGDPR
                     AAERTFQLLHQVVGRAGREEGRGFGYLQTHQPEHPVMR"
     gene            1878..2813
                     /locus_tag="VFL49_07895"
     CDS             1878..2813
                     /locus_tag="VFL49_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET7911938.1"
                     /translation="MSEFLVAPDISAEIARWLSHLGAERRMSAKTLDAYRRDVFQFMS
                     FLTEHLGRAPTLKQLAKLTPADIRAFMAARRSDGAGNRSLMRTLAGCRSFTRFLERNG
                     KGKVAALAAVRTPKLPKTLPKPLAVSAAKKISDTELRDGEEREPWIIARDAAVLVLLY
                     GCGLRVSEALGLKGKDFTGKCDSLIVTGKGNKTRMVPLLPQVSKAIADYLVLCPYKNP
                     PNGPLFIGARGKSLSPRIVQLTMARLRGALGLADTATPHALRHSFATHLLARGGDLRS
                     IQELLGHASLSTTQIYTGVDAERLLEVYNSAHPRA"
     gene            2876..>3148
                     /locus_tag="VFL49_07900"
     CDS             2876..>3148
                     /locus_tag="VFL49_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HET7911939.1"
                     /translation="MGAHENLEHAEHAEHASHSNKKIALLISVLALFLAFSETLGKSA
                     QTAAIADNIKSSDAWNFFQAKTIRQTSLRIAADAMAVQLPSITNEES"
BASE COUNT          648 a          987 c          930 g          583 t
ORIGIN      
        1 cgcatcaccg gatgctccgg ctgatgcgtc tgcaggtagc cgaaaccgcg cccctcctct
       61 cgcccggcgc gcccgaccac ctgatgcagc aactggaatg tgcgctccgc ggcacgaggg
      121 tcgccgtttc ccaggccaag gtcggcatcg acgataccga ccagattaag cttcggaaaa
      181 tgatgccctt tggcgacaag ctgcgtgccg ataacaagat cgaagcgtcc ttcggcaaca
      241 tcgtcgagct cttgccgcag ccggtcgatc gattccacga gatcgctcga cagcacgaga
      301 atgcgggcct gtggaaacag ttcagccgcc tcgtgcgcga gccgttccac gccgggcccg
      361 acggcagcaa gactttcggc agcatggcaa tttgggcatt ccgccggcgg cggaatcgaa
      421 tatccgcagt gatggcacac gagcctgcgc ttgaaccgat gatcgaccag ccaagcatca
      481 cagttcggac actggaaccg gaacccgcac gtccgacaca gcgtgagcgg tgaataaccg
      541 cgacggttga gaaaaagcag cgcctgctgg ccgcgcgtta acgaaacggc tacttcttca
      601 gcaagccgcg ctgaaataaa gcgcccgcgc ggcggtccct cgcgtcgcat gtcgatcgcc
      661 tcgatcgacg gcagatgcgc cccaccgaaa cgttcgggca gatgcacgtg tcgatagcgc
      721 ccgcgccgcg cattgacttc ggattcgact gaaggcgtcg ccgacgcaag cacaaccgga
      781 attttcgcga tcgaaccgcg cacgacggcc atgtcgcgcg cgtgatagtg cgcaccatct
      841 tcctgcttgt aagccgggtc gtgctcttca tcgacaacga taagtcccag gtcggcatag
      901 ggcagaaaca gcgccgagcg ggcgccgaca atcaccgaga cttcattcgc agcaacggcg
      961 gcccaggtct gtgcacgctt gcgctgcgaa atttgcgaat gccattcggc aggacgcgtg
     1021 ccgaagcgtc cggtgaaccg atcgaggaat tgtgcggtca gcgcgatttc cggcatcagg
     1081 atgagagtct gccgcttgtg ccggatattt tcggcgaccg cttcgaaata aacttcggtc
     1141 tttcccgacc cggtgacacc gtcgagcagg gtcaccgtat agccgccctg ctcgacggtc
     1201 ttgcgcaagc cgtcggccgc ggcaagctgc gccagcgaga attccggttt tctgaaatcg
     1261 ggatcggacg gcaacgcgac gggatcgggc ggtatcacca ccgtttccag cgtgccctca
     1321 tcgatcaatc cattgatgac gcttgcactg acaccggctt ccttcgcggc ggagcacttc
     1381 gggcgggcaa atccatcagc aacaagccgg agtacacgcg cgcgtgcagg agtgatccgc
     1441 tgtggcggct gcccaacaaa gcggacgcca acctgctcac gccccgggcc gaggtgctca
     1501 cccatgcgca gtgccatacg cagcacggtg ccgcgagacg caagggtata gccagaaacc
     1561 cagtcgacga attgtcgcag ttcggccttg agcggcggca cgtcgagctt ctcgtcgacg
     1621 tctttcaatc ggttgtgcaa tcgcggattt ggcgcggagt tttccgccca aaccacgccc
     1681 atgcattcgc gtgccccaag tgacaccgaa acaaggtttc ccggtgcaag ctcgagcccc
     1741 gccggaaccc gataggaata ggtccggtca agcgccaccg gcaccagaat atcgacgacg
     1801 cgcatggtca tggctcaggt ctggccgatt ccgtggcttt aaggaagggt gaagaaagca
     1861 atgccactct tgccgccatg agcgaattcc tcgtcgcgcc agacattagt gccgaaatag
     1921 cacgttggct gagccatctt ggtgccgaac gccgcatgtc agcgaaaacg ttggatgcct
     1981 atcggcgtga cgtatttcag ttcatgagct ttttgaccga acacctgggc cgcgccccaa
     2041 cgctgaagca actcgcgaaa ctgacgccgg ccgatattcg cgccttcatg gccgcacggc
     2101 gcagcgatgg tgccggcaat cgctcattga tgcggacgct tgccggctgc cgttctttca
     2161 cgcggttttt ggagcgaaac ggtaagggaa aagttgcggc gcttgctgcg gtgcgcaccc
     2221 caaaactgcc gaaaacattg ccaaaaccgc tcgctgtttc tgccgccaag aagatttccg
     2281 atacggaatt gcgtgacggc gaagaacgcg agccgtggat catagcgcgc gacgcagccg
     2341 tactcgtgct gctttacggc tgcggcttgc gggtttccga agccctcggc ctgaagggaa
     2401 aggacttcac cggcaaatgc gatagcctca tcgtcaccgg aaagggcaac aaaacacgga
     2461 tggtgccgtt gcttcctcag gtctcgaagg cgatcgccga ttaccttgtg ctttgcccgt
     2521 acaaaaatcc tccgaacggg ccacttttta tcggggcgcg aggcaaatcc ctgtcacccc
     2581 gcatcgtgca gttgacgatg gcacggcttc gcggcgcgct cgggctcgcc gatactgcca
     2641 ctccgcatgc cttgcgccat tcattcgcga cgcatttgct cgcgcggggc ggcgacctgc
     2701 gatccattca ggagttgctc gggcacgctt cgctatcgac cacccagatc tacacggggg
     2761 tcgatgccga gcgtttactc gaagtttata acagtgccca tccgcgcgcc tagcgcgccc
     2821 tcttgcgcct ggctgacctc tgctatcatc gtgactcgaa gtacgggagg gggcgatggg
     2881 cgcgcacgaa aatctggagc atgccgaaca cgccgagcat gcatcgcatt cgaataagaa
     2941 gatcgcgctg ctgatctccg tgctggcgct gtttcttgcg ttctccgaaa cgctgggtaa
     3001 aagcgcgcag acggcggcga tcgccgataa catcaagtcc tccgatgcct ggaatttctt
     3061 tcaggcgaag accatccggc agaccagcct gcgcattgct gccgacgcta tggcagtgca
     3121 gttgccgtca atcaccaatg aggagagc
//
LOCUS       DASSRL010000387         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_762477, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000387 DASSRL010000000
VERSION     DASSRL010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762477"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFL49_07905"
     CDS             complement(<1..895)
                     /locus_tag="VFL49_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492607.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7911940.1"
                     /translation="MPSDLAAQARSARVAFYNDPKVRSVAYQLILCVVVALLVYGAAR
                     NAIDNLARAQIASGFGFWNQTAGFDISQTLIEYSSRASTYGRAFWVGLLNTLLVAGLG
                     IVFATIIGFVVGISRLSGNWLLAKAAGGYVEIIRNLPLLLQLLFWYNAVLKTLPDIRE
                     SIVIPGGVILNNRGFFLPMPVLKGGIGLFELSLFAGIVGAVAFYVWARKRQERTGQRA
                     PVLAVTLLLVIGLPLAVFALAGRPIGFEFPQAGRFNITGGIEVLPEFVALLFGLSIYT
                     AAFIAEVVRAGILAVSRGQTEA"
     gene            complement(983..2005)
                     /locus_tag="VFL49_07910"
     CDS             complement(983..2005)
                     /locus_tag="VFL49_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7911941.1"
                     /translation="MKQLIAVTVAVSAGVMLAAGTAQAGATLDAVKKRGQVECGVNTG
                     LAGFGIADSQGKWTGLDIDFCRAVAAAVLGDANKVGYKPLNASQRFTALQSGEVDVLA
                     RNTTWTSSRDTSLGLNFVGVNYYDGQGFMVRKSLKVNSALELNGASVCVQQGTTTELN
                     LADYFGAHKMQLKSVTFATANEAVKAYDAGRCDAYTTDASALYAERLRVANPDDHVIL
                     PEIISKEPLGPVVRHGDDQWFDIVKWTLFAMINAEELNVSSKTLDEALKSPNPEIKRF
                     VGTEGNYGEQLGLTKDWAVRIVKQVGNYGESFERNVGMGSPLKIERGLNKLWTKGGIQ
                     YAPPIR"
     gene            complement(2167..>2868)
                     /locus_tag="VFL49_07915"
     CDS             complement(2167..>2868)
                     /locus_tag="VFL49_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7911942.1"
                     /translation="ETVNAHPTRYAGFAALPTADPKASADELERAVTRLGFKGAMVHG
                     LTNGVFFDDKRFWPIFERAEKLDVPLYLHPALPHPAVVEAYYKDYLKDFPAILTAAWG
                     FTVETATQGIRLILSGVFDAYPNLKIILGHLGESLPFSLWRIDQGLSRASKMPRTFRD
                     YFCTHFWITTSGNFSTPALLCSMLEMGVDRVLFSVDYPYVMNAPGTKWMDGVPLSAED
                     KEKILSGNAKRLLRL"
BASE COUNT          549 a          908 c          915 g          496 t
ORIGIN      
        1 ccgcctcggt ctggccgcgc gacaccgcca aaatgccggc gcgcaccact tcggcgatga
       61 aggccgccgt atagatggac aggccgaaca gcagcgcgac aaactccggc agcacctcga
      121 tgccgccggt gatattgaaa cgtccggcct gcggaaactc gaagccgatg ggacggcccg
      181 ccaaggcgaa aaccgcaagc ggcaatccga tcaccagcag caaagttacc gctaaaaccg
      241 gcgcgcgctg ccccgtccgt tcctgccgct tgcgggccca gacataaaag gcaaccgcac
      301 cgacaattcc ggcaaacagg gacaattcaa agagccctat accgcctttg aggaccggca
      361 ttggcagaaa aaagccgcgg ttgttgagaa tgacgccgcc gggaatgacg atgctctcgc
      421 gaatgtcggg aagcgtcttg agcacggcgt tgtaccagaa cagcagctgc agcagcagcg
      481 gcaggttgcg gatgatctcc acatagccgc cggcggcttt ggccagaagc caattgcccg
      541 acaggcgtga aatgccgacc acaaagccga tgatcgtcgc aaacacgatg ccgaggccgg
      601 caaccaaaag cgtgttgagc agtccgaccc agaacgcgcg accataggtc gaggcgcgcg
      661 aagaatattc gatcagcgtc tggctgatgt cgaagccggc ggtctggttc cagaatccga
      721 acccggatgc gatctgcgcg cgcgcgagat tgtcgattgc gtttcgcgca gcgccataga
      781 cgagcagcgc gaccaccacg caaagaatga gctgataggc aacgctgcgg accttcgggt
      841 cgttgtaaaa tgcgacacgc gcggagcgcg cctgcgctgc caggtcactc ggcatttccc
      901 cctcgtcggg cgtctcgacc ggcggcgact cccggtgaca aagccgccgg tcgcccagcg
      961 cagccggccg ttttgcctgc gctcagcgga tcggcggggc gtactggatg ccgcctttgg
     1021 tccacagttt gttcaggccg cgctcgatct tgagcggcga tcccatgccg acattgcgct
     1081 caaacgactc gccgtagttg ccgacctgct tgacgatgcg gactgcccag tccttggtaa
     1141 ggcctagctg ttcgccatag ttgccctcgg tccccacaaa gcgcttgatc tccgggttcg
     1201 gagatttcag tgcctcgtcg agcgtcttcg atgagacgtt caattcctcc gcattgatca
     1261 tcgcgaagag cgtccacttc acgatgtcga accactgatc atctccgtgg cgaaccacag
     1321 gcccaagcgg ttctttggag atgatctccg gcaaaatgac gtgatcatcg ggattggcga
     1381 cgcgcaatcg ctccgcatag agcgccgaag cgtccgtcgt ataggcgtcg caccgccccg
     1441 cgtcataggc cttcacggcc tcgtttgcgg tggcgaaggt gacgctcttg agctgcattt
     1501 tgtgtgcgcc gaaataatcg gcgaggttga gctcggtcgt ggtgccctgc tgtacgcaga
     1561 cggaagcgcc gttgagttcc agcgccgagt tgaccttgag cgacttgcgc accatgaagc
     1621 cctggccgtc ataatagttg acgccgacga agttgagacc gagcgaggtg tcacgcgacg
     1681 acgtccaggt cgtgttgcgc gccagcacgt cgacctcgcc cgactgcagc gcggtgaagc
     1741 gctgcgaggc gttgagcggc ttgtagccga ccttgttggc gtcgccgagc acggccgcag
     1801 ccacggcgcg gcagaaatcg atgtcgaggc cggtccattt gccctggctg tcggcgatgc
     1861 cgaagccggc gaggccggtg ttgacgccgc attcgacctg gccgcgcttc ttgaccgcgt
     1921 ccagtgtcgc gcccgcctgg gcggtcccgg ccgccagcat gacgcccgcc gacacagcga
     1981 cggtcactgc aatcaactgt ttcatgatcg tctccccagg tttgcgatgg cggattgtgg
     2041 ccggcggcgg ccgccgctca cgccggctga cggccatcat cgacgacgcc gtcggccctt
     2101 ggcaacagcg aacgtcgtaa caaaaaagtc tctatccagc ccgtcgtcgg ttgcccgtgc
     2161 cgcggcctag aggcgcagca gccgcttggc gttgccgctc aggatcttct ccttgtcctc
     2221 ggcgctgagc gggacgccgt ccatccattt cgtgcccggc gcgttcatga catagggata
     2281 gtcgaccgag aacaggacgc ggtcgacgcc catctcgagc atgctgcaga gcagcgcggg
     2341 cgtcgagaaa ttgccgctcg tcgtgatcca gaaatgggtg cagaaatagt cgcggaacgt
     2401 ccgcggcatc ttgctcgcgc gcgacagccc ctggtcgatg cgccacagcg agaacggcag
     2461 cgactcgccg agatggccga ggatgatctt gaggttcgga taggcgtcga acacgccgct
     2521 caggatcagg cggatgccct gcgtcgcggt ctcgacggtg aagccccagg cggcggtcag
     2581 gatggccggg aagtccttga ggtaatcctt gtaataggcc tcgacgacgg ccggatgcgg
     2641 cagcgccggg tgcagataga gcggcacgtc gagcttctcg gcacgctcga agatcggcca
     2701 gaagcgcttg tcgtcgaaga acacgccgtt ggtcaggccg tgcaccatgg cgcccttgaa
     2761 gccgagcctg gtgacggcac gctcgagctc gtcggccgaa gccttggggt cggcggtcgg
     2821 cagcgcggca aagcccgcat agcgcgtcgg atgcgcattg accgtctc
//
LOCUS       DASSRL010000388         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_640262, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000388 DASSRL010000000
VERSION     DASSRL010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640262"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFL49_07920"
     CDS             complement(<1..485)
                     /locus_tag="VFL49_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911943.1"
                     /translation="MANVLVGRLSVGRPFRTFLKVLAAGGAAVSISSAASAALVYDTS
                     LASPGFYNGTGNPNEGFTVNTQGGIELGLGVNNRFIGPVHPTSTNQYDVAIGFSTVPL
                     AKWNFEYSVNVAGAGLTLGQIHTLLTITNLANLQSLSFDPFLVPDNAHFDGTTTFNGG
                     GA"
     gene            complement(638..1195)
                     /locus_tag="VFL49_07925"
     CDS             complement(638..1195)
                     /locus_tag="VFL49_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HET7911944.1"
                     /translation="MAMTRAIAFAAALMLAAGTSFADNPQPYAGLQNRPLKALSDEQL
                     DDLKAGRGMRLALSAELNGYPGPKHVLELATELNLTEPQRARTQQLYDAMKAEAVPLG
                     QQLIAAEADLDRQFSGRTITPANLTAASAKIGTMQGQLRAAHLKYHLSMMDVLTPGQI
                     RRYGELRGYGAAPAGHSPGMHNHSK"
     gene            complement(1233..1484)
                     /locus_tag="VFL49_07930"
     CDS             complement(1233..1484)
                     /locus_tag="VFL49_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911945.1"
                     /translation="MRLDVESCRDSGRELPRAFRLKRRRVTVTEIVDQWFGPDYRYCK
                     LKGDDGAVYILRVVEHCSDWQLTLFSSPSIQAVAASSGT"
     gene            1589..>2075
                     /locus_tag="VFL49_07935"
     CDS             1589..>2075
                     /locus_tag="VFL49_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HET7911946.1"
                     /translation="MSEIDAIRALLTAKPRPVGWRERRERIDEIGSTWSVAGDVRLEE
                     VDLDGVPGEWSAVPGSDASRVLLFFHGGGYCSGSIVSHRRMVTEAGRAARMRTLAVDY
                     RLAPEHPYPAAHEDAMTAWRFLRRQGIEARHIAVGGDSAGGNLTVTLINRLRAAGEEQ
                     PA"
BASE COUNT          382 a          619 c          672 g          402 t
ORIGIN      
        1 gcgccgccgc cattgaaggt ggtcgtgccg tcaaagtggg cattatcggg gacgaggaaa
       61 gggtcgaagc ttaaggactg aagatttgcg aggttggtga tcgtcaacag cgtgtgaatc
      121 tgcccgaggg tgagtcctgc gccggcaacg ttgaccgagt actcgaaatt ccacttggcg
      181 agcgggacgg tcgaaaatcc gatggccacg tcatactggt tcgtggaggt cggatgcacc
      241 ggtccaatga agcggttatt tacgcccaga ccgagctcga tgccgccctg tgtattgacc
      301 gtgaagccct cgttcggatt accggtgccg ttataaaaac ccgggctggc caatgacgtg
      361 tcgtacacaa gtgcagcact ggcggcgcta gagatcgaaa cagcagctcc accggccgca
      421 agaactttca gaaatgttcg gaatggacgt cccacggata atctccccac cagcacgttc
      481 gccacgaaaa aaacctagca ggtatgcgac caccagcaat atatttttgc agattcaact
      541 gcataattag aaattgttag tttgtgattg caactctcaa tcacgagtac cgacgacgtg
      601 ttggcgtgac ggcgcatacg cccgcgatgt gctcgtctca cttgctgtga ttgtgcattc
      661 ccggcgaatg ccccgccggc gcggcgccat agccccgcaa ttcgccgtag cgccggatct
      721 gcccgggcgt gagcacgtcc atcatcgaaa ggtggtactt caggtgcgcg gcgcgcaatt
      781 gcccttgcat cgtgccgatt tttgcggagg cggccgtaag gttcgcgggc gtgatggtgc
      841 ggccggaaaa ctggcggtcg agatcggctt ccgccgcgat gagctgctgc cccagcggaa
      901 cagcctcggc cttcatcgca tcgtagagct gctgcgtgcg cgcgcgttgc ggctcggtca
      961 gattcaattc cgtcgcgagc tccagaacat gtttcggtcc cggatagccg ttgagctccg
     1021 ccgacaatgc cagtctcatg ccgcggcccg ctttcagatc gtccagttgt tcgtcggaca
     1081 gcgccttgag cggcctgttc tgcagtcccg cgtagggttg cgggttatca gcgaaagatg
     1141 tgccggctgc aagcatcagc gcagcggcaa aagcgattgc gcgcgtcatg gccatgacct
     1201 ccgaattgcc gccgactata tcaccgttgc gcttatgtcc ccgaggacgc tgcgacggcc
     1261 tggatgcttg ggcttgagaa cagcgtgagc tgccagtcag agcaatgctc gacgacgcga
     1321 aggatataga cagcgccgtc gtcgcccttg agcttgcaat agcgatagtc ggggccgaac
     1381 cactggtcga cgatctccgt caccgtgacg cggcggcgtt taagacggaa cgcgcgcggc
     1441 aactcccgtc ctgagtcacg gcagctttcg acgtcgaggc gcatttcagt cctccgcttt
     1501 gcccgtttag ggtaggggtc acgcagcctg cggacaaggt tcacagcggg ctgttatgtt
     1561 cacgctccat tgcttgaggg cataatccgt gagcgaaatc gacgccattc gcgcgctgct
     1621 gacggcaaag ccgcggccgg tcggctggcg cgagcggcgg gagcgcatcg acgagatcgg
     1681 ctcgacctgg tcggtcgccg gcgatgtgcg gctcgaggaa gtcgatctcg atggcgtgcc
     1741 gggtgaatgg tcggccgtgc ccggcagcga cgcttcgcgc gtgctgctgt tcttccacgg
     1801 cgggggctat tgctccggct cgatcgtcag ccatcgccgg atggtgaccg aagcgggccg
     1861 cgccgcgcgc atgcgcacgc ttgcggtcga ctatcgcctt gcgccggagc atccttatcc
     1921 ggcggcgcac gaggacgcga tgacggcctg gcgcttcctg cgcaggcagg ggatcgaagc
     1981 gcgccacatc gcggtcggcg gcgacagcgc cgggggcaac ctgaccgtca cgctgatcaa
     2041 ccggctgcgc gctgcgggcg aggagcagcc ggcct
//
LOCUS       DASSRL010000389         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1025260, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000389 DASSRL010000000
VERSION     DASSRL010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025260"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1223)
                     /locus_tag="VFL49_07940"
     CDS             complement(138..1223)
                     /locus_tag="VFL49_07940"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease, SacI family"
                     /protein_id="HET7911947.1"
                     /translation="MARSIDYDAARKVLKRAFASAEADYRDGKKVEVSKKIANASRTL
                     FLSRTQAYREALVGCVLAYAQDPTIDIRYPNAKHSTNAFSGRTLDEQVVNPFFQDRAV
                     PVSKNPYLSAIRRGVHFVPGGARGQRDQDAFNALVDIVNYVRTRSKDDALAYLRYLLG
                     LFVALRESAKITLRRVARLSLAQYSPLIDGLLSTPSGGLMPVLLAVAMLRTLRDCFQL
                     AWDIEWQGINVADRQSGASGDITVRRAGQTFLVVEVTERPITRDRVVSTFNTKISPSG
                     LDDYLFFFAAVQPTDDARAAARRYFAQGHEINFIPIRDWIVTCMTTIGPNCRAAFTSN
                     VLLLLESKDVPAAVKQTWNDQVTALLD"
     gene            complement(1233..2108)
                     /locus_tag="VFL49_07945"
     CDS             complement(1233..2108)
                     /locus_tag="VFL49_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET7911948.1"
                     /translation="MTRREVSPIFLDDPASDGRSFVVARPRRRRIVFGWYGGKFNHLD
                     WLLPLLPAAHHYCEPFAGSAAVLLNRIPAPVETYNDVDGDVVNFFRVLRDQPETLARA
                     IALTPFSREEFHNAIADDAGQASGLERARRFYIRARQARTGLAQTATLGRWANCKDTS
                     RAGMSGVVSRWLGGVGALPEIGTRLIRVQIENRPALDVLRLYDSPTTLFYCDPPYLHD
                     TRGDSSAYGYEMEQGEHVALAEALNSCKGKVALSGYRNKLMDKLYKGWRRFDAPAKQC
                     HSIKKVRQESLWMNY"
     gene            complement(2263..>3068)
                     /locus_tag="VFL49_07950"
     CDS             complement(2263..>3068)
                     /locus_tag="VFL49_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7911949.1"
                     /translation="PAAALEVSMIHSVVGQLSGGRLIRNRPFRDPHHSASLPALVGGG
                     QRARPGEISLAHLGVLFLDELPEFQRATLEALRQPMESGRVSVARVNAHVTYPARVQL
                     VAAMNPCPCGHLDDPAQACGRAPRCAQDYQARISGPLFDRIDLHIDVPAVSAADLTLP
                     PPAEGSAEVAARVATARAIQAARYKAKGNGIRTNAECDGELLEEVAALDEPSRKLITQ
                     AIERFRLSARGFHRVLRVARTLADLEGGGPVKQIHVAEALTYRRVLPGR"
BASE COUNT          574 a          953 c          947 g          594 t
ORIGIN      
        1 aggggcattg tgtttcaata tagccgcaag tctggctaac atccggcatg atcgcgccag
       61 cttctgtcag cgccacttat gcgcacttgc gcagcaggaa cgccatctcg gggactccgg
      121 ttctgttcga ggtcggatca atccaaaaga gccgtcactt gatcattcca ggtttgcttg
      181 acggcagctg gcacatcttt gctttccagc aagagcaata cgtttgatgt gaatgcggcg
      241 cggcagtttg ggccgattgt tgtcatgcag gtgacgatcc aatcccgtat cgggataaaa
      301 ttgatctcgt gcccttgcgc gaagtatctc cttgccgcgg ctctcgcatc atccgtcggc
      361 tgaactgcag caaagaagaa caagtaatcg tcaagcccgc tcggggatat cttcgtattg
      421 aaagtagaga ccactcgatc tcgagtgatc gggcgctcgg tcacttcaac gacgaggaac
      481 gtttggccgg ctcgccggac ggtgatatct ccgctcgctc ccgactgccg gtctgcgacg
      541 ttgatcccct gccattcgat gtcccaagca agctgaaaac agtcgcgaag agtcctcaac
      601 atcgcaacag cgagcaggac cggcatgaga ccgccgcttg gcgtagacaa taggccgtca
      661 atgaggggag agtattgagc cagactcagg cgtgccactc ggcgcagcgt aatctttgcg
      721 ctttctctca gggcaacaaa cagcccaagt agatagcgca ggtatgcgag cgcgtcgtcc
      781 ttgcttcgcg tgcgaacgta gttaacgatg tcgactagcg cgttaaaggc gtcttggtct
      841 ctctgtcccc gggcccctcc cggaacgaaa tgtacccctc gacggatcgc cgagagatat
      901 ggattttttg agaccggtac agctctgtcc tggaaaaacg gattcaccac ctgttcgtcg
      961 agcgtacgtc cgctaaacgc attcgtgctg tgctttgcat ttggatacct gatatcgatc
     1021 gtcgggtctt gcgcatatgc aagaacgcaa ccgacaaggg cttcgcgata tgcttgggtc
     1081 cgcgacaaga agagcgtccg acttgcgttc gcgatcttct tggaaacctc aaccttcttg
     1141 ccgtcgcgat aatcggcttc agcagaagcg aaggcccgtt tcaggacctt tcgcgcagca
     1201 tcatagtcga tcgagcgggc cacgacagcc tatcaataat tcatccagag agactcctgt
     1261 cggaccttct tgatcgagtg gcattgcttc gccggcgcat cgaaacggcg ccagccctta
     1321 tagagcttgt ccatcagctt gttgcggtac ccggacagcg ccaccttgcc cttgcaggaa
     1381 ttgagcgcct ccgcgagggc gacatgctcg ccctgctcca tctcgtatcc gtaggcgctg
     1441 ctgtcgccgc gcgtgtcgtg caagtacggc ggatcgcaat agaacagcgt cgtgggactg
     1501 tcgtagagcc gcagcacgtc gagcgccgga cgattctcga tctgcacgcg gatcagccgg
     1561 gtgccgatct ccggcagagc cccgaccccg ccaagccagc gcgacacaac acccgacatg
     1621 ccggcccggc tggtgtcctt gcagttcgcc catcgcccca gcgtggcggt ctgggcgagg
     1681 ccggtccgcg cttgccttgc cctgatgtag aaacgcctag cccgctccag cccgctcgct
     1741 tgtcccgcgt catcggcaat cgcgttgtgg aattcctccc gcgagaaagg ggtcagagcg
     1801 atggcccgcg caagcgtctc cggctgatcc cgcaggacgc gaaagaaatt caccacgtcc
     1861 ccatcgacgt cgttgtaggt ctcgaccggg gccgggatgc gattgagcag gacggcggcc
     1921 gagccggcga atggttcgca atagtgatgg gccgcgggca ggagcggaag cagccaatcc
     1981 aggtggttga acttgccgcc gtaccagccg aacacgattc gtctgcgccg cggccgggcg
     2041 acaacaaagg atcgtccgtc cgacgccgga tcatccagaa agatgggtga aacttcgcgc
     2101 cgcgtcatat ttgcattcct cccggcagct accttggatg gtggcccatt ccccgcttga
     2161 acgcaacatg tgcccaggtc tcatgctgca tcaagctgat gttctccgat tgttctatag
     2221 ccaggtgatt gaaagatcaa tagtcgtttc taccgtaagt ctttaccgcc ccggcagcac
     2281 ccgcctatac gtcagggctt ccgccacgtg gatctgcttc accggcccgc cgccttcgag
     2341 gtcggcgagg gtgcgggcga cgcggagcac gcggtggaag ccgcgcgccg acaatcggaa
     2401 gcgctcgatc gcctgggtga tgagcttgcg gctgggctcg tcgagcgcgg cgacctcttc
     2461 cagcaattcg ccgtcgcatt cggcgttggt gcggatgccg ttgcccttcg ccttgtagcg
     2521 cgcggcctgg atggcgcggg ccgtggcgac gcgggccgcg acctcggcgc tgccttccgc
     2581 cggcggcggc agggtcaggt cggcggcgct caccgccggc acgtcgatgt ggaggtcgat
     2641 gcggtcgaac agggggcctg agatgcgcgc ctggtagtcc tgcgcgcatc tgggcgcgcg
     2701 cccgcaggcc tgtgcgggat cgtcgagatg gccgcacggg cacgggttca ttgccgcgac
     2761 cagctgcacg cgcgcggggt acgtgacatg cgcattcacg cgcgcgaccg agacgcggcc
     2821 cgattccatc ggctgacgca gagcctccag cgtcgcgcgc tggaactccg gcaattcgtc
     2881 gaggaagagg acgccgagat gggcgagcga gatctcgccc ggcctggcgc gctgcccgcc
     2941 gccgaccagg gcgggcagcg aggccgaatg atgcggatcg cggaacggcc ggttgcggat
     3001 cagccgcccg ccggataatt gccccaccac cgaatggatc atggaaacct cgagtgccgc
     3061 agccggat
//
LOCUS       DASSRL010000390         3976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_255070, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000390 DASSRL010000000
VERSION     DASSRL010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255070"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFL49_07955"
     CDS             complement(<1..443)
                     /locus_tag="VFL49_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7911950.1"
                     /translation="MVGQQNNLPSVASKTRRPAAFLDRDGVLNVDHGYVHRPDQLEWI
                     LGAAESVRMLNEAGYYVLVITNQSGVARGFFDEAAVISFHAHMQNVLAGTGARIDAFY
                     YCPHHPEGTVKALAMACSCRKPRTGMLEQAAREWPIDMSRSFLIG"
     gene            complement(462..1436)
                     /locus_tag="VFL49_07960"
     CDS             complement(462..1436)
                     /locus_tag="VFL49_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7911951.1"
                     /translation="MRRTLLLLAKATVSGFLLYFSFRWVNLDALIERLHRLELGWLLL
                     AIVLLAIQLLPLSARWRQIAIACGTDLGFAGTLQINFIAAFFNQMLPSTVGGDGVRIW
                     FAARRGAGWVNATYSVLLDRIFGVFVLALLVAACLPWTLQLIREPVARAVLLVLAFGA
                     ITGPLTVMLASTHFHRWFRRWMITRHVAAAAEAAASVCRSFGSAAVVLGCSIMIHLLT
                     ISAAWCCAKAVAAPVGFAHVLFLMPPVLLIATVPISIAGWGVRESSMIAAFAYAGLAE
                     SDGLTLSILFGAVSFMIGLVGGLIWIVSGFRTPTFATAADAETAAGRP"
     gene            1646..2887
                     /locus_tag="VFL49_07965"
     CDS             1646..2887
                     /locus_tag="VFL49_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET7911952.1"
                     /translation="MNITSTNRAPSVPTGARRADLTVVGGAGHVGIPLVLALADAGLI
                     VNVNDLNETALATLNSGRLPFIEHGAAPLLARALESQRLVFTSSPKDIGRAGPVIVTI
                     GTPVDEFLNPVQAEIQRCIDGLLPHISDEQLLILRSTVYPGTTDWLQGHLSRLGRRNG
                     LAFCPERVVQGYGVKELREMPQIISATTPDAEQEASALFRRIVPELVVVKPIEAEFAK
                     LFNNAYRYIEFAATNQFYMIAKSAGADYQAIMRAMKQNYPRARNFPGPGFSAGPCLFK
                     DTMQLAAYARNQFGLGHAAMQVNEGFVLQLVDDLRRSYDVSKMTIGLLGMAFKAEIDD
                     TRASLSYKLKRSLEMLAHEVLTTDPFVTTDPALLPLEQVITRSDILILCTPHSVYAGA
                     DLKGKPVVDVWGFLKNANVVY"
     gene            2994..>3976
                     /locus_tag="VFL49_07970"
     CDS             2994..>3976
                     /locus_tag="VFL49_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET7911953.1"
                     /translation="MKITRLTPRQILIVLHDLLATAVAIVATFLLRFEGPALTERLHG
                     LVTFLPAFLVYAAAVYFVIGLHRNKWRFTSVPDLYNLFRASTVLALSLLALDYVLLAP
                     NVYGTFYFGKLSILLYWFLQMFFLGGLRVVYRYFHYARTLQRVKIADATPTLVLGRAA
                     DAEVLLRSIESGAVKKIWPVGVLSPSSADRGQSIRGLRVLGDIEDLEQVVADLERRGE
                     RVTRLVLAPSALTPEARPEAILIRARKLGLSMSQLPSLDAEGPAVQLAPVAVEDLLLR
                     PSVKIDYRRLENFVSAKAIVVTGGGGSIGSEICDRIVTFGASRLLVIEHSEP"
BASE COUNT          821 a         1186 c         1174 g          795 t
ORIGIN      
        1 tcacctataa ggaaactcct gctcatgtcg atcggccatt cgcgcgcggc ctgctcaagc
       61 atgccggtgc gcggctttcg gcaactacag gccatcgcaa gggctttcac cgtcccttcc
      121 ggatggtgtg ggcaatagta aaaggcatcg atgcgagcgc ccgttccagc cagtacgttc
      181 tgcatatgcg cgtgaaaaga tatcactgct gcctcatcga agaacccgcg cgccacaccg
      241 gactgattgg ttatcacaag cacgtaatac ccggcctcat tgagcatgcg cacgctttca
      301 gccgctccca ggatccattc aagctgatcg ggccggtgga catagccatg atcgacattg
      361 agaacgccgt cgcgatcgag gaacgcggcc gggcgtcgcg tcttggacgc gaccgaaggc
      421 aaattgttct gttggccgac cattgctaag cagttccgga gttacggcct tccggcggct
      481 gtttcggcgt ccgccgccgt tgcgaatgtc ggcgtccgga atccgctgac aatccagatc
      541 aatccaccga ccagcccgat catgaaactg acggcgccga acaggatgga cagcgtcaga
      601 ccgtcgctct ccgcaagccc ggcataggca aatgctgcga tcatgctgct ttcgcggaca
      661 ccccagccgg cgatcgatat cggcacggta gcgatcagca gcaccggcgg catcagaaag
      721 agaacgtgcg caaacccgac cggcgcggca accgctttgg cacagcacca ggcagcgctg
      781 atcgtcaaca gatgaatcat gattgagcat ccgagcacga ccgcagcgga cccaaacgat
      841 cggcaaacgc ttgcagcggc ctcagcggcc gccgccacat gacgcgttat catccatcgg
      901 cggaaccatc gatgaaaatg cgtgctggcc aacatgactg tcaatggacc cgtgatcgcg
      961 ccgaacgcga gcaccagcag gaccgcgcgc gcgaccggct cacggataag ctggagcgtc
     1021 cagggcaggc acgcagcgac cagaagtgcc aggacgaaaa cgccaaagat tcgatcgagc
     1081 agaacggagt atgttgcatt cacccagcct gcacctcgcc gggcggcgaa ccagattcgc
     1141 acgccgtcgc cgccgacagt cgatggcagc atctgattga aaaacgcggc gatgaaattg
     1201 atctgcaacg tgccggcaaa acccaggtcg gtgccgcagg caatggcgat ctgccgccag
     1261 cgcgccgaca gcggcaaaag ctggatcgcc aggagaacga tggcgagaag cagccagccc
     1321 aattcgagtc gatgcagccg ctcgatcagc gcatcgaggt tgacccaacg aaaggaaaaa
     1381 tagagcagga agccggatac cgtcgctttg gccagcaaca aaagcgtgcg gcgcatgtga
     1441 tcctcaggtt tccttgacgg tcgctggatg gagcgagggc ttggcatgac caatatgtgc
     1501 gcgcaagaga attgccgcta ttgtccaaca gtggaatttg atagctaagt gtctggaaat
     1561 ccgtcgatta gttcgtgtat gcagacgcgc gggtctccca gaactttgtt aaagtggcat
     1621 tccagaccgg acgcgagtgg atcgtttgaa catcacatcg accaaccggg cgccttccgt
     1681 cccgacgggc gcaaggagag ccgatcttac cgtcgtcggc ggcgcgggcc acgtcggtat
     1741 tccccttgtg ctggctcttg cggatgctgg cctgatcgtc aatgtcaacg atctcaacga
     1801 gacagcgctt gcgacgctga attcggggag gctgccgttt atcgagcatg gcgcggcacc
     1861 gcttctggcg agagcgctgg agagtcagcg gcttgttttc acgtcatcgc caaaggacat
     1921 cggccgcgcg gggcccgtca ttgtcacgat cggcacaccg gttgatgaat tccttaatcc
     1981 cgtgcaagcc gaaatccagc gatgcatcga tggtctgctt ccgcacatca gcgacgagca
     2041 attgctgata ttgcgctcca ccgtctaccc gggaacgacc gactggcttc agggccatct
     2101 tagccggctt ggccgcagga acgggcttgc attctgtccg gaacgggtgg tgcaaggata
     2161 tggcgtcaaa gaactgcgcg agatgccgca aatcatcagc gcgaccacgc cggatgccga
     2221 gcaggaagca agtgcgctgt tccgcaggat cgttcctgag ctcgtcgtcg tcaagccgat
     2281 cgaggccgag ttcgcgaagc tcttcaataa tgcctaccgg tacattgaat ttgcggcgac
     2341 caaccagttc tacatgatcg caaaatcggc cggtgcggac taccaggcga tcatgcgcgc
     2401 catgaagcag aactatccgc gcgcaagaaa ctttccggga ccgggttttt ccgcgggccc
     2461 ctgtctgttc aaggacacaa tgcagcttgc cgcctatgcg cgcaatcaat tcggtctcgg
     2521 ccatgccgcg atgcaggtca acgagggttt tgtgcttcag ctcgtcgacg atctacgccg
     2581 gtcatacgat gtctcaaaaa tgacgattgg cctcctgggc atggcattca aggccgagat
     2641 cgacgacaca cgggcctcgt tgagctacaa gctcaagcga tcgctggaaa tgctggcgca
     2701 tgaggttctc acgacggatc cttttgtgac gacggatcct gcattgctgc cccttgagca
     2761 ggtgatcaca cgcagtgata tcctgatcct ctgcacgcct cactccgttt acgcgggcgc
     2821 cgatcttaaa ggaaagcccg tcgttgacgt ctggggtttt ctcaaaaacg caaatgtcgt
     2881 gtactgagca tgcctgcatg cgttgaagtc atcaaaattt ccgtgtgtgg aatcttcgct
     2941 ggccgtcatt tggccggtgg cacgaaacgt cacttggcgc ggaaggctgc gaaatgaaaa
     3001 taacaagact tacgccgcgg caaattctga ttgtcctgca cgatctgtta gcgacggcgg
     3061 tcgcgatcgt agctaccttt ttgctgcggt tcgagggtcc cgcgcttacc gagcgtctgc
     3121 atggcctggt gacattcttg ccggcctttc tggtctacgc tgctgccgtc tatttcgtta
     3181 tcggcctgca ccggaacaag tggcgattta cgtcggtccc ggatctctac aatttattcc
     3241 gggcatcgac cgtacttgcg ctgtcgctgc tcgcgctcga ctacgttctt ctggcgccga
     3301 atgtctacgg gacattctat ttcggcaaac tctcgatcct gctgtactgg ttcctgcaga
     3361 tgttctttct gggcggcctc cgtgtcgtct accgatattt ccactacgcg cgaacgctgc
     3421 aacgcgtgaa gatcgcggac gcgacgccga cgcttgtcct cggccgtgcc gccgatgccg
     3481 aagttctgct gcggagcatc gagagcggag cagtcaagaa gatctggcct gtcggcgtgc
     3541 tctctccgtc gtcagcggac cgcgggcaat cgattcgcgg cttgcgcgtg ctgggcgata
     3601 tcgaggacct tgaacaggtt gtcgccgatc tggagcgtcg cggggagcgc gtgacccggt
     3661 tggtgcttgc gccttccgcg ctcacacctg aggcgcggcc ggaagcgatc ttgatccgcg
     3721 cccgcaagct tggcctcagc atgagtcagc ttccgtcgct cgatgccgag gggccggcgg
     3781 tccagcttgc gccggtcgcc gtggaagacc ttttgttgcg gcccagcgtg aagatcgact
     3841 accgccggct tgaaaatttc gtaagcgcaa aagctatcgt cgtgacgggc ggcggcggct
     3901 cgatcggctc cgaaatctgt gaccgcatcg tcactttcgg agccagccgc ctgctggtga
     3961 tcgagcattc cgagcc
//
LOCUS       DASSRL010000391         5008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_955403, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000391 DASSRL010000000
VERSION     DASSRL010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955403"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            705..1055
                     /locus_tag="VFL49_07975"
     CDS             705..1055
                     /locus_tag="VFL49_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911954.1"
                     /translation="MTYFIAAQQNAKASIYESPQGYSDASRTGCRLAEDGDAARPIPD
                     FHDRIDGSFQDFTGFQQSIGVVGALTRLGGRNVVALKQMEPIAGGHGPLPVIRFSEAE
                     LQECSTTGVKDAIG"
     gene            complement(1349..1480)
                     /locus_tag="VFL49_07980"
     CDS             complement(1349..1480)
                     /locus_tag="VFL49_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911955.1"
                     /translation="MASNESKSAAREENEIFWRATLALLVVLVLGIGSVAALGVLTL"
     gene            complement(1779..2021)
                     /locus_tag="VFL49_07985"
     CDS             complement(1779..2021)
                     /locus_tag="VFL49_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911956.1"
                     /translation="MIISARVGLGDLAGPQIADRQDQRRHGAAGMAPSAQGVDTAHER
                     KSEIQHDRIEIDGVAEEVPLPAVERRFASRLRRVPR"
     gene            2388..4568
                     /locus_tag="VFL49_07990"
     CDS             2388..4568
                     /locus_tag="VFL49_07990"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7911957.1"
                     /translation="MLPVSGTFEEDTGLWWLFTMRGAIQPPAQAVVVAIDGTTGKDLD
                     LPKLPRDWPRLIHAQLIENLVARGADVIVFDMDFGRVKGAYEDAVFANAISKANRVVL
                     FEPLVGKKESVESNEGKTVTWTEQKLSPSPQLAATARALGPFPLPKLGRTAFEFWAFK
                     PSVGDAPTTPAIALQLYAMRYYDRWLAALEQARVLGIKQLPANPRDVRRAPDTRSLMN
                     MLRNALGGDPATRERLQQTIDRIGGLQDEERRVLKALSALYTGPDSRNINFYGPPGTI
                     ERIPYQAVIKPELQLATPHQVDLKGKVVFVGYSDLADPEQPDRFYTVFTGEDGVDLSG
                     VEIMATAFANLLTDRSLRPSSPAATIGIVLAFGFTIGAALYLLPAMIGVAFGLAASAM
                     YVAGAQWSFNSADIWLPLVTPLLVQLPIAILIGLITQYLVGRRLERRMSRAISYYLPA
                     NIVKDLTEKHLDPSQANKVVYGACLATDMSGFTAIAEKKSPTELAKFMNAYFDEVADA
                     LKRHSVDITNFHADTIMCAWIGTDQNSPARKKAVLAALDVMQAIERFGRRDDSFKLNA
                     RIGLQDGNFYVGHTGGGGHLNYSILGDPANTASRLESLNKELGTHILAAQSVVADLTS
                     GFLTRPLGSFRLVGKSDAIPVSEIVAQRLGATDSQIDLCNRFSHALDVLQARQWQEAA
                     ALFNAILEEYEDDGPSRLYAARCRQLADDAAPEADPTVIHLDRK"
     gene            4808..>5008
                     /locus_tag="VFL49_07995"
     CDS             4808..>5008
                     /locus_tag="VFL49_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911958.1"
                     /translation="MRRASAIASLGAQVLLLVGVLPTLLAGVASAESCAPVVGELVSV
                     EGQVQIRRADTADWRQAQLNELL"
BASE COUNT         1008 a         1530 c         1490 g          980 t
ORIGIN      
        1 caggtggctg agcatagcga cagtctaggt gaatgacagc gatgtggcgc gcagcaccac
       61 cgtgccggca ccgccgcatg gtagatcgcg tgaggcggag agagccgcgc cgccgcgacg
      121 tgctgtgggt cgggcgcgac agccctcgac atcagtgtcg gctgaaattg actcctgtcg
      181 ggacaaatga cctaacacat tgatattgct agacgatccg aaatcggcga ctgcaggaaa
      241 ccgtaccgtt ggcgcgacct cattgccaag cgcgagatgc gtgaattgca tggccccagc
      301 ggtcccgaca cgccgaccgg cagccccgcc ttacgcaggg cggcaaaaag aactcttcca
      361 acgccggatc tttgaaagga agcgcctcgc gtcgagcggc tgtcgtgaac cggggctcca
      421 gcctcatcgc ttctgcccgc gccacatcag cctggtctcg ctgatccagc atgatgggca
      481 cggccgcccg caagcggagc agacccgggt tcacctggcg gatcggcgtc gttcgggttg
      541 agaccgtagg cgcgttcata agcggcctgc gcgccgctga agtccgcttt cacacacagc
      601 acgtcggccg gcatccaatg agtccagcgc gatcgccaag tcggcgaagt atctcgcgcc
      661 gctcggatcg agctcaacgg cattctcaag cagggtacgc gcagttgact tacttcatcg
      721 ccgcgcaaca gaacgcaaag gcaagcatct acgaatcgcc gcagggatac agcgatgcat
      781 cacgaaccgg ttgcaggctt gctgaggacg gcgacgcagc gagacccatt ccagacttcc
      841 acgacaggat agatggaagc tttcaggact tcaccggctt ccaacaaagc atcggcgtcg
      901 tgggcgcact cacacgcctt ggcggccgca atgtggtcgc cctcaagcag atggagccga
      961 tagccggcgg acatggccct ctccccgtca tcaggttctc cgaggccgaa ttacaggaat
     1021 gctctacgac gggagtcaag gacgcgattg gatagcggcg aggcgttaat ttgcgattcg
     1081 caaagttatc gcgccgcagg ccggagcgat gtgcggtccg tcttcccaat acgacgcatt
     1141 ccttcccacg cctcggcggc ttggaactca gggcgctgcc tttagagggt ctcacgggtg
     1201 attgagtacc gcgtctccgt ccgtctcaca ttccaacggc tcgtcgtcat gtatgggccg
     1261 gtcctgaccc aacggatagt atcgacagct cgttatcatc acatttcggt tggtaatggc
     1321 tcgcgccctt cgagagacgg ggggccgctc aaagcgtgag cacacccaaa gcagcaaccg
     1381 aaccgatgcc gagcaccaaa acgaccagca gcgccagcgt tgcgcgccag aagatctcgt
     1441 tttcttcacg cgccgccgac ttgctctcgt tgctggccat ttgttgcccg aatatgcctg
     1501 agtgctggtg cactcagata gatcagcacc ggttaaatta ctgttgagcc agatccgcgc
     1561 tacagagatc gcgccatttt cggatgagcg ttcaaacagc ggcgccgtga agcacttcga
     1621 ttcaatgcct cgatggcgac cctggtcgat cggcgtacag ttgccgtgct tcccagtcga
     1681 agaagtttgt tatcgctgcc attcggccga ccgtcaaagc tgcgagatcg caatccgcat
     1741 gctggtcatt gacaaccgat cccaatctga acggcaaatc agcggggcac tcgtcgaaga
     1801 cggctggcga atcggcgctc gacggcgggc aagggcactt cctcggcgac accatcgatt
     1861 tcgatgcggt cgtgctggat ctcggatttc cgttcatgag cggtatcgac gccttgcgca
     1921 gatggcgcca tgccggccgc tccatgccgg cgctgatcct gcctgtccgc gatctgcggt
     1981 ccggcaaggt cgccgagacc gacgcgggca ctgatgatca tgtcgccaag cggtttcaca
     2041 tgctcgagct gatggcgcgc ttgcgggcac tgatacgccg cagcgcgggt ggccctgatc
     2101 ccgtaatgac aagcggttcg atcaggttcg acactcgaag cggaccggcc aaagcggagg
     2161 caaaacggcg gccactctgc ccgcctcgaa gtgtggccgc ttggagcgag gcgcgggggc
     2221 cagcaccgca agcccaggcg tacgtcgcct tgactttgaa atcctcttcc tttgatagta
     2281 tttgacccca atcttatgca gtgaagcggt ggacgggccg gatgcgcagg tgggggcaga
     2341 gcatcattac tggcattgcg acggccgcat gcggggcggc ctttgccatg ctccccgtca
     2401 gcggcacctt cgaggaagac accggccttt ggtggctctt cacgatgcgt ggcgcaatcc
     2461 agcccccagc ccaagccgtt gttgtcgcga tcgatggaac gaccgggaag gatctggatc
     2521 tacccaagct tccgcgcgat tggccgcgat tgatccacgc ccaattgatc gagaacctcg
     2581 ttgcccgcgg cgccgatgtg attgtctttg acatggattt cggcagggtg aagggcgcgt
     2641 acgaggatgc ggtgttcgcg aacgccatca gcaaggccaa ccgggttgtc ctcttcgagc
     2701 cgctcgttgg gaagaaggag tcggtcgagt ccaatgaggg gaagacggtc acgtggaccg
     2761 aacagaaact ctccccctct ccccagctcg cggcgacggc cagggccctt ggtcccttcc
     2821 cattgcccaa gctcggtcgc accgctttcg agttctgggc atttaagccg agcgtgggcg
     2881 atgctccgac gactcctgca atcgccctcc agctctacgc gatgcgatac tacgacaggt
     2941 ggctggcggc tctcgaacag gctcgcgtgc tgggcatcaa gcagctaccc gcgaatcctc
     3001 gtgacgtgag gagggctccc gacacacgaa gcttgatgaa catgctgcgg aacgctcttg
     3061 gcggggatcc ggctaccagg gagagactcc agcaaacgat cgatcgcatt ggcggactcc
     3121 aggacgaaga acgccgcgtg ctgaaggcgc tctccgctct ctacaccggc ccagacagtc
     3181 gaaatatcaa cttctacggg ccgcccggaa cgatagagag aattccgtac caggcagtga
     3241 tcaagccgga actgcagttg gcgacgccgc accaggttga tctgaaaggc aaggtggtat
     3301 ttgtcggcta ttccgatttg gccgacccgg aacagcccga ccgtttctac accgttttta
     3361 cgggcgaaga tggtgtggac ctcagcggcg tcgaaatcat ggcaaccgcc tttgccaacc
     3421 tgctcaccga tcgctcactg cggcccagct cgccggctgc cacgatcggc attgtactcg
     3481 cattcggatt cacaattggc gccgcgttat atcttctgcc ggcaatgatc ggtgtcgcct
     3541 tcgggttggc cgcgtctgcc atgtacgtgg ccggcgctca gtggtcgttt aactccgcgg
     3601 acatttggct gcccctggta accccgcttc tcgttcagct gccaatcgcc attctgatcg
     3661 gcctaatcac gcaatatctc gtcgggcgcc gcctggagcg acggatgagc cgtgcgatca
     3721 gctattacct gcccgcgaac atcgtcaaag acctaaccga aaagcacctt gatcccagcc
     3781 aggcgaacaa ggtcgtctat ggtgcctgcc tcgccaccga catgtccggc ttcaccgcca
     3841 tcgccgagaa gaaatcgccc acagaactgg caaagttcat gaatgcgtat ttcgatgagg
     3901 tcgccgatgc gctcaagcga cactcggtcg acatcaccaa tttccacgcc gacacgatca
     3961 tgtgcgcctg gataggaacg gatcaaaatt caccggcgcg taagaaggct gtcctcgccg
     4021 ccctggatgt aatgcaggca atcgagcgat ttggcaggcg agacgattcg ttcaagctga
     4081 atgcacgcat cggcttgcag gatggcaact tctatgtcgg ccacacgggt ggcggtggcc
     4141 atctgaatta cagtatcctc ggcgatccgg cgaacacggc atctcgcctg gagagcctga
     4201 acaaggaact tggaactcat attcttgcgg cacaatccgt tgtcgccgac ctcacatccg
     4261 gttttctgac ccgcccgctg ggctcgttcc gtctcgtcgg aaaaagcgac gcgatacctg
     4321 tctcggaaat tgtggcacag cgcctgggag ctaccgacag ccaaatagat ttgtgcaacc
     4381 gattctcgca cgccttggat gtgctgcagg cgcgccaatg gcaggaagca gccgcgctgt
     4441 tcaacgcaat cctcgaagag tacgaggatg atggtcccag ccgcctctac gctgcccgct
     4501 gccgccaact tgcagatgac gcggcgccgg aagccgatcc cactgtcatt catcttgatc
     4561 gcaagtaagg cattcctgtt gacagggaac tgcaatcgtt catacttgtc gcaactccaa
     4621 aaaaatcttc gcactgttca aaacgacatc gcttcggtaa cgcgtcgccg gaagtatttt
     4681 tgcagtcgcc atgcggagcc cgatctggtt ctgctgtctt ggggattgaa gggcgacact
     4741 gcacgaaggg ccagataggg ccggcgccgt ttgcacgtga agtccgttca ccaagatggg
     4801 gcgggcgatg cgtagggctt cggctattgc gtccttgggg gcgcaggtat tgctcctcgt
     4861 cggggttctt ccgactctgc tcgcgggcgt ggcaagcgct gagtcgtgcg cccctgttgt
     4921 cggcgagctg gtgtctgtcg aaggtcaagt gcagatacgg cgcgccgata ctgccgattg
     4981 gcgacaggcg cagctcaatg aattgctc
//
LOCUS       DASSRL010000392         7410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_185278, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000392 DASSRL010000000
VERSION     DASSRL010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185278"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFL49_08000"
     CDS             complement(<1..196)
                     /locus_tag="VFL49_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HET7911959.1"
                     /translation="MSGNQKKPLIVGITGASGAIYGVRLLELLRKCGIETHLIVSRAA
                     VLTLAYETDLKLSDLAKLAPV"
     gene            complement(370..1632)
                     /locus_tag="VFL49_08005"
     CDS             complement(370..1632)
                     /locus_tag="VFL49_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HET7911960.1"
                     /translation="MIEDRKKIIDTIDANREKAIKFLQDIVSIPSVTGDEGKIQAFLD
                     KYLKKIGLDVDKWESNWKKLKKHPGYIPVEQGYEGRPNIVATWKGKGGGRSLLLNGHT
                     DVIPVGNGEGWSDDPWSAKVKDGRIYGRGSCDMKSGVASHILAVEFLKKAGIKLQGDV
                     YINIVVDEEISGHGTLDTVIRGYKADAGISGETSGLAVQPACIGRIWFEIEIQGKPAG
                     IQRRYQGVSGIELGYKITKAVEALEAKRVASVKHPLYPKAIDALPCMIGAFHAGNFPS
                     SFPATCLLKGSMATVPGEDHDGVKRSLVEHIAKTAAEDPWMKDHPPKVRFVGYDAQAS
                     EIPVSHPIVDTVSKNYTEVTGRPPEISGRQGAADTRFLNLYGSTPTVIFGPGSTAVMH
                     SDDEYVSVDDYLTAIKVMALSIYDWCGD"
     gene            complement(1705..2880)
                     /locus_tag="VFL49_08010"
     CDS             complement(1705..2880)
                     /locus_tag="VFL49_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7911961.1"
                     /translation="MNTVSLTLNGRVVSAEVEPRMHLGDFIRETQTLTGTHLGCEHGV
                     CGACTVLVDDVPVRSCITYAVACSGASVTTIEGLDEDEIGKELRAAFTREHALQCGYC
                     TPGMLISARDLVVRAEMPSEEDIRIAMSGNLCRCTGYVGIIRAIQNVIADRRARGISG
                     VPGAGRSVLGPAGSGHGQTIVANASVVAPSASVVPQNVGDESLADWKPQASFGRSFIV
                     NHTIGEVWDFFADTNAVASCLPGASLSGDTSARNIRGKMRLKVGPIAAEFHGMAQIDR
                     DAATYSGAIHGSGRDQRSNSATRGVIRYRLLPQGQAATKVELNVGYLLTGPLAQFSRS
                     DLIQDIAGRLIATFAQNVEAWLSGSARAPQSAEFNAVGLLFSALADRVKFYVRRLFG"
     gene            complement(2877..3704)
                     /locus_tag="VFL49_08015"
     CDS             complement(2877..3704)
                     /locus_tag="VFL49_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET7911962.1"
                     /translation="MKPVKFQYARPANLSAAIELGGRDDVIVKFIAGGQSLGPMLNLR
                     LVQPDILVDITAVPDLKRIEQRPESLFIGACVTHADIEDGRVPDVCRGVMRTVAAGIA
                     YRAVRNRGTIGGSLIHADPSADWISALAVLGAQAVVRDREQERRLPVEKLMTGVFESS
                     LSQGELLIGVEVPRLSPNARWGYFKHWRKTGEFAHAIGAYMHDPDRALRRAVIGATGT
                     RPIVLTDTTALFGGKPDGEIDRDFAKHAMADNGVTDPIEQQIHFACLRRAIAQARRA"
     gene            complement(3701..5983)
                     /locus_tag="VFL49_08020"
     CDS             complement(3701..5983)
                     /locus_tag="VFL49_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7911963.1"
                     /translation="MRGRGQFVADLRFAGMKDVAFVRSPLAHGRIRGIAVPDAHKGAV
                     FTATDLDGVRPIRAVSGLRGFKASDQPSLAADKVRYVGELVAMCVAPTRAEAEDIASS
                     VNLDIDELPAVYDMLAARDPASALLHEHWGDNVFLETFVDVNIDKVLDAPIKVTREIR
                     TSRQCMAPIEGRGVVAIWESRPEQLTIYSACQMPHIVRTGLAGCLGLDEAQVRVVAPD
                     VGGGFGYKGILLAEEVCLAWLAMHCGHPVRWIEDRREHLTAGANCREHHYRITGYADR
                     EGRLLGIDCEATVDSGAYSAYPFSACLEAAQVASILPGPYDFPGYRCRTYSVATNKCP
                     ILPYRGVARTGVCFALELVLDAIAREAGVEPHEARRKILVGPEKMPFTNITKKWFDSG
                     DYPESLRRAVAAIDIEAVRARQKRGEPDGRLVGVGMATYCEQAAHGTSVYYGWGIPMV
                     PGFEQATARLTPDGGLELRVGIQSHGQGLETTLAQVAHEILGIAVAKIKVVHGDTAMT
                     PYSTGTWGSRCMVMAGGAVSVACKEIAKRAAKIGAQLLQTDVDSVKVGGGEVSGPQAS
                     VSLTEIARTWYLKPQNLPSDVDMGGLEATVGYRPSPDSGTFSYATHAALVAVDPEIGD
                     VKILDYVIVEDGGKLVNPMIVDGQILGGFAQGVGTALYEEMPFNSAGQPLASTFADYL
                     LPGPTEVPAARIEHMETLSPHTEFGVKGIGEGGAIAPPAAIANAVNDALRPLRAELLV
                     SPITPRQVLAAIARAGGSAA"
     gene            complement(6185..6982)
                     /locus_tag="VFL49_08025"
     CDS             complement(6185..6982)
                     /locus_tag="VFL49_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7911964.1"
                     /translation="MSELSKVYDRSRVPLYIQVASVMRRRIETKHWLPGQKISTLVEL
                     EREFEVARVTIRQAIDILREEGLLHSQQGRGTFVAETSISRHWFKLATSWDVLVDTIK
                     DNVLKRIKVNNPPATPTLREGEGELAEKYVFMRSVQFKDGEPYGVVNVHLADGVFRRA
                     PDDFRQHPALSVLAGLKGIEVEDAHQTVVIGSADPVTAGLLHIELGAPIAECRCVVID
                     EKGVAIYVADIIYRSDAIKLHIDLLASARASSRSGARATKARLGALA"
BASE COUNT         1586 a         2275 c         2021 g         1528 t
ORIGIN      
        1 caaccggggc aagcttcgcc aggtcagaaa gtttaagatc tgtttcgtaa gcgagcgtca
       61 gcacggcagc gcgcgatacg ataaggtgag tctcaatgcc gcattttctg agcaactcta
      121 gtagccgaac cccgtagatg gccccggacg caccggtgat gccgacgatc aacggctttt
      181 tttgattacc gctcatgcct gctctcgtga tgtcaaccaa cggcatgatc gtgccgtcca
      241 aattcagtct gcggctgatt tgcgtaacct agttaatcgt atcgcacctt cctaatcatg
      301 cccataggat cgagcgccta aaggacaaac catcatcagc tatagcctct gtcgcaattt
      361 tcgagcggct cagtcgccac accaatcata tatactcaat gccattacct tgatcgcagt
      421 aagatagtcg tcaaccgaca catattcatc gtcggaatgc atgacagcgg tcgaccctgg
      481 tccgaagatt acggtcggtg tgctaccgta aagattgagg aagcgcgtgt cggcggcccc
      541 ttggcgaccg ctgatttccg gcggacgtcc ggtcacctcg gtgtaattct tactgacagt
      601 atcaacaatc ggatgagata ccggaatttc cgaggcttgc gcgtcgtagc ccacgaaccg
      661 cactttcggc ggatggtcct tcatccaggg atcctcggcg gcagtcttcg cgatgtgttc
      721 gacgagactt ctcttcacgc catcatggtc ctcgccggga acggtcgcca tgctgccctt
      781 caataggcag gtcgctggga atgaactggg aaaattgccc gcgtggaaag ccccgatcat
      841 acatgggagc gcgtcgatag ctttgggata aagcggatgt ttgacgctcg cgacacgctt
      901 agcctcaagc gcctccactg ctttggttat tttatagcca agctcgatcc cacttacgcc
      961 ttggtagcgt cgctggatac ctgccggctt tccctgaatt tcgatttcga accaaatgcg
     1021 gccaatgcag gccggctgca ccgccaggcc gctggtttct ccggaaatgc cagcatcagc
     1081 cttgtagcca cggatcaccg tatccagcgt gccgtggccg ctgatttctt cgtcgaccac
     1141 gatattgatg tagacatcgc cctggagctt gatgccggcc ttctttagga attcaacagc
     1201 gagaatgtga ctcgccactc cactcttcat gtcgcacgaa ccgcggccat agatccgtcc
     1261 atccttgact ttggccgacc agggatcgtc gctccaacct tcgccgttgc cgaccggaat
     1321 aacgtcggta tgcccgttca gtagcagcga gcggccgcca cctttacctt tccatgtggc
     1381 aacaatatta gggcggcctt catagccctg ctcgactgga atatatcccg gatgcttctt
     1441 cagctttttc cagtttgact cccacttatc gacgtcaaga ccaattttct tcagatactt
     1501 gtcaagaaac gcctggatct taccttcgtc gcccgtaacg ctggggattg atacgatatc
     1561 ctgcaggaac ttgatggcct tttccctgtt agcgtcgatt gtatcgatga ttttcttacg
     1621 atcttcgatc atctgatctc tccataagca atactcggtc atctcatagc tccaaaaatc
     1681 agcgcgctcg catgggcatc tcattcagcc aaacaaccga cgcacataaa atttcacgcg
     1741 atcagcaagc gctgagaaaa gcaggccgac ggcattgaat tctgccgact ggggcgcacg
     1801 cgcgctaccg ctgagccacg cttctacatt ttgcgcaaaa gtcgcgatca gacggccggc
     1861 aatatcctga ataagatccg aacggctgaa ttgggctagc ggccctgtca gcaggtagcc
     1921 gacgttgagt tcaaccttgg tcgcagcctg tccctgcggc aagaggcgat agcggataac
     1981 gccacgcgtc gcagagttgc ttcgctgatc gcggccactg ccatggattg cgcccgagta
     2041 tgtcgccgcg tcgcggtcga tctgggccat accgtgaaat tcagcagcaa taggtccaac
     2101 cttgagccgc attttacctc tgatgttgcg ggcgctcgta tcgccgctca gcgaggcgcc
     2161 tggaagacat gatgcgactg cattggtatc ggcgaagaaa tcccaaacct caccgatcgt
     2221 gtgattgaca atgaaacttc gaccgaagct cgcctgcggc ttccagtcgg ccaggctttc
     2281 atcgccgaca ttctgaggca caaccgacgc cgagggggcc acaacgctcg cgttcgcgac
     2341 gatcgtctga ccgtggcctg acccggctgg accaagtact gaccgccctg cccccggtac
     2401 gcccgagatt ccgcgtgcgc ggcggtcggc aatgacattt tggatcgcac ggattatgcc
     2461 gacataacct gtgcagcggc aaagattgcc actcatcgca atgcggatat cttcctcgga
     2521 tggcatttct gcccgaacca ccagatcgcg agccgaaata agcatgcccg gggtgcaata
     2581 gccgcattgc aatgcgtgtt cgcgggtaaa agccgcacgc agttctttgc cgatttcatc
     2641 ttcgtcaaga ccctcaatgg tggtcacgct ggccccgctg caagccaccg cataggtaat
     2701 gcacgaccgc acgggaacgt cgtcgaccag cacggtgcag gccccgcaga cgccgtgttc
     2761 gcagccgagg tgcgtccctg tcaaagtctg cgtctcgcgg atgaagtccc ctagatgcat
     2821 ccgcggctca acttccgcgc tgaccacgcg tccgttgaga gtaagcgaga ccgtattcat
     2881 gcgcggcgtg cctgcgcaat cgcccgcctc aggcaggcaa aatggatttg ctgttcgatc
     2941 ggatcggtca caccattatc cgccatggca tgcttcgcaa agtcgcggtc gatttcgccg
     3001 tcaggtttgc cgccgaacag agccgtcgta tcggtcaata caatcggcct tgtccccgtc
     3061 gcgcctatga ctgcgcggcg aagcgctcga tcaggatcgt gcatataggc tccgatggcg
     3121 tgcgcgaatt cgcccgtctt gcgccagtgt ttgaaatatc cccaacgtgc attgggcgac
     3181 agtcgcggca cctcgacacc gataagcagt tctccttgcg acaggctgct ttcaaagaca
     3241 cccgtcatca gcttctcaac cggaagacgc cgttcctgct cccggtcacg cacgacggcc
     3301 tgcgcaccga gtacagccaa tgctgatatc caatctgccg aaggatcggc gtgaatgagg
     3361 ctacccccga ttgtcccgcg gttgcgtaca gcgcgataag cgatgcctgc ggcgaccgtg
     3421 cgcatcacgc cgcggcaaac gtcgggcacg cggccgtctt cgatgtcagc atgggtgacg
     3481 catgcgccga tgaacagtga ctctggtctc tgttcgatac gtttgagatc aggaacggct
     3541 gtaatgtcga cgaggatatc cggctgcacc agccgcaaat tgagcatagg gccgagcgac
     3601 tgcccgccgg cgatgaattt cactattacg tcgtcgcgtc cgccgagctc tatcgccgcc
     3661 gacaaattgg ccgggcgggc gtattggaat ttgaccggct tcacgccgca ctcccgcctg
     3721 cgcgcgcaat cgccgcaagc acctgtcgcg gcgtaattgg tgatacgagc agctcagcac
     3781 gcaatggacg aagcgcatca ttgacagcat tggcaatcgc tgcaggaggc gcgatcgctc
     3841 cgccctcgcc tatgcccttg acgccgaatt cggtatgcgg cgaaagcgtt tccatatgct
     3901 caatgcgcgc agctggcacc tcggtgggtc ccggcagcaa atagtccgca aacgtcgaag
     3961 cgagcggctg acccgccgaa ttgaacggca tctcttcgta aagcgcagtc ccgacgccct
     4021 gcgcaaatcc gccaagaatc tggccgtcca cgatcattgg attgaccaac ttgcccccgt
     4081 cttcgacgat aacgtagtca agaattttga catcgccgat ttccggatcg acagcgacca
     4141 gcgcggcgtg tgtggcgtaa ctaaacgtgc cgctgtccgg tgatggacgg tagccgactg
     4201 tggcttccaa tccgcccatg tcgacgtcgc tgggcagatt ttgcggcttc aaataccagg
     4261 tgcgtgcaat ctcggtgagc gacacggacg cttgcgggcc ggaaacctcg ccgccgccta
     4321 ccttcacgct atcgacgtcg gtctgcagta attgtgcgcc gatcttggcc gcgcgtttgg
     4381 cgatctcctt acaggcgacc gagaccgcgc cgcccgccat aaccatgcac cgcgaacccc
     4441 aggtacccgt cgagtatggt gtcattgcgg tgtcgccgtg cacgaccttg atctttgcaa
     4501 cagcgatgcc gagtatttcg tgcgccactt gcgcgagcgt ggtctcgaga ccctgaccgt
     4561 gcgactgaat gccgacgcgc aactccaagc caccatcagg agtcagccgt gcagttgcct
     4621 gttcgaaacc gggcaccatg ggaatgcccc agccgtagta aaccgacgtg ccgtgggcgg
     4681 cctgttcgca ataggtcgcc atgccgacac cgacaagccg gccatcgggc tcgccccgct
     4741 tttgccgcgc tcgcacagcc tcgatatcga tcgcagccac ggctcggcgc agggattccg
     4801 gatagtcgcc gctgtcaaac cacttcttgg tgatattggt gaacggcatc ttttccggac
     4861 ccaccaaaat cttgcggcga gcttcatgtg gctcgactcc ggcttcccgc gcgatggcgt
     4921 cgagaacaag ttcaagcgcg aagcacaccc cggtccgcgc gaccccgcga taaggcaaga
     4981 ttggacattt attggtcgcg accgaataag tgcggcaacg atatccagga aagtcgtaag
     5041 gcccagggag aatgctcgca acctgcgccg cttcaaggca ggccgagaaa gggtacgcgg
     5101 aataagcacc agaatcgact gtagcttcgc aatcgattcc aaggagacga ccttcgcgat
     5161 cggcatagcc tgttatccgg tagtgatgtt cgcggcaatt ggcgccggca gtcagatgct
     5221 cgcgccgatc ctcgatccag cgcaccggat gaccgcaatg catggcaagc caggcgagac
     5281 atacctcttc ggccagcagg attcccttgt agccgaaacc accaccaacg tcgggagcga
     5341 cgaccctgac ctgcgcttcg tccaagccga ggcagccggc aagcccggta cgaacgatgt
     5401 gcggcatctg acatgctgaa taaatagtga gctgctcggg gcgactctcc caaatcgcaa
     5461 ccacgccacg gccttcaatc ggcgccatgc actggcgcga agtacggatt tctcgcgtca
     5521 ccttgatggg agcatcgagt actttgtcga tgttcacatc aacaaaggtc tccagaaaga
     5581 cgttgtcacc ccagtgctcg tgtagcaatg cagacgcagg atcgcgcgca gccaacatat
     5641 catatacagc tggaagctcg tcgatatcga ggttaaccga gctcgcgatg tcctccgctt
     5701 ccgctcgcgt tggggcaacg cacatggcga caagctcgcc aacgtagcgg accttgtcgg
     5761 ctgcaagaga cggctgatcc gaagccttga acccgcgcag tcccgagacc gcgcggatgg
     5821 gtctgacccc atcgaggtcg gtggcagtga agaccgcacc cttatgggcg tcgggaacgg
     5881 caatgccacg gatgcggcca tgcgcgagcg ggctgcgcac aaaggcgacg tccttcatgc
     5941 ctgcaaaccg cagatcagcg acaaactgac cgcggccgcg catcagacgg tcgtcttcct
     6001 tgcgaagaac gcgtgcgccg acgccttgcc ctgtcacagc aaccaaattc cccagtcaca
     6061 catcatggca cacgcgggga aaccgccatg tcgtcgcgat cgagcggcgc ccggcaattc
     6121 agcattcaac gttctatgat gctaatctta gtatgacatc aattgcaact acgaaggccc
     6181 ccggtcacgc cagggctccg agccgagcct ttgtcgctcg cgcgcccgac cgcgacgacg
     6241 cgcgagcgct agcaagcaag tcgatgtgca acttgatggc gtcactgcgg tagatgatgt
     6301 cagcaacgta gatcgccacc cctttttcgt cgataaccac acatcggcac tcggcgatcg
     6361 gtgcgccgag ttcgatatgg agcagtccgg ccgttaccgg atccgcgctg ccaatcacca
     6421 cggtctggtg cgcatcttca acctcaatgc ctttcaaacc cgccagaacc gacaaggccg
     6481 ggtgctgccg gaaatcatcc ggcgcgcgcc ggaatacgcc gtctgcaaga tggacgttga
     6541 cgacgccgta gggttcgccg tctttgaact gtacgctgcg cataaacaca tatttctctg
     6601 cgagttcgcc ctcgccctcg cggagcgttg gggtcgccgg cggattattc actttaatgc
     6661 gtttgagtac attgtccttg atcgtgtcga ccagtacatc ccagcttgtt gcaagcttga
     6721 accagtggcg ggagatcgaa gtttcagcga cgaatgttcc tcgaccttgc tgcgaatgca
     6781 gaagtccttc ttcgcgcaga atatcgatgg cctgacgaat ggtgacgcgc gcaacttcaa
     6841 actcgcgctc caactccacc aatgtcgaaa tcttttgacc gggaagccag tgcttcgtct
     6901 caatccgccg tcgcatgacc gaagcgacct gaatgtagag aggaacacgg ctacgatcgt
     6961 aaactttcga cagttcggac atggtacctt cgatccgcca atacggattc ctaattttat
     7021 ctgatcgagt gggccatcac ggtgtatcga atataaaagc ccacccccgt cgcccttgat
     7081 tgctcctcag cacgtgcaac aggtttatcg acgcacgatc gcgaggagga gcaactacca
     7141 ggcgcagcga tagagacggc attcgcaaat taggagcagc caattccttt cgtcgacagt
     7201 tgccgccgcg gatgcaccgc gaaacggcac gcaatcaccc atcacttacg acctttcggt
     7261 gttgctatca aactaaaaac aagcacaata ccgcaccggg ttaaagtccc ctttgcaggg
     7321 cgaggcaggg ctgatggcct gatggcaaag tgtggaattg accccggtag caaatgctag
     7381 gtgggcggaa acgcgacgac gttcttgccg
//
LOCUS       DASSRL010000393         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_27578, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000393 DASSRL010000000
VERSION     DASSRL010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27578"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFL49_08030"
     CDS             <1..413
                     /locus_tag="VFL49_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HET7911965.1"
                     /translation="IPQLALRVNAAAGAQPSGTLPLEIQGTGKETRSFCFIDDLIAGV
                     MIMREKGEHLGIYHVGTMEEVTIADLAHRIAGLYGREIALTPGKLAPGGTGRRCPDIS
                     KLAKLGYKPRVPLSEGLRPTLGWYRQNAERASKA"
     gene            420..1700
                     /locus_tag="VFL49_08035"
     CDS             420..1700
                     /locus_tag="VFL49_08035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7911966.1"
                     /translation="MTDVPRPSKIARAAGTGASVPVECCQVCGHAPLETVLSLGYMPP
                     VNQMVPIGQVQRQLPWFPTALLHCRKCDLVQLGLAVDPVIIFPPEYPYTSGTTKLLRD
                     NFADLQRESVAMLGLTGEDLVIDIGSNDGTLLSNFKNGGQRVLGIEPTDVGDIANQRG
                     IPTIKRYFGLDTARQVKREHGPASVVTAANCFAHMEDVHAIVDGIVEMLKPDGVFISE
                     SHYLVPLLDTLQYDTIYHEHLRYYSLTSLRHLLDMHGLEVFHARPIPSHGGSIRVYAA
                     RRGSRPVQDSVGRMLAAEPHGEAMARRLQAFRRDVILSKLRLLSMLRDLKEKGARIAG
                     ISAPSRASTLVNYVGLDEGLIDYVCEIAGSLKIGKYMPGTQIPVVDEFRLFTDQPDCA
                     VIFSWHIADERAPKLKAKGFRGKLITPLPEPRVL"
     gene            1711..>2192
                     /locus_tag="VFL49_08040"
     CDS             1711..>2192
                     /locus_tag="VFL49_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7911967.1"
                     /translation="MTAGHAQDKNKEIAFFDAHAEHDAYDVFTPQANERIIAAFMRLS
                     GLPRGARVADLGCGSGVFTELLRHQGFQSVGLDISPRLIQLGRRKYPGLELIEGDAEN
                     LPFESASLDGVLLSGLVHHFPDPRRLAGEVSRVLRSGGRFVAFDPNRMNPFMWLYRDR
                     S"
BASE COUNT          412 a          712 c          661 g          407 t
ORIGIN      
        1 tgattccgca gctggcgctg cgggtgaatg ccgccgccgg tgcgcagcct tcggggacgc
       61 ttccgcttga gattcagggc accggaaaag agacccgcag cttctgcttt atcgatgacc
      121 ttatcgccgg cgtcatgatc atgcgtgaga aaggcgagca tctcggcatc taccatgtcg
      181 gcaccatgga agaagtgacg atcgcggacc tcgcgcaccg gattgcaggc ctttacggac
      241 gtgagatcgc tttgaccccg ggaaaactgg caccgggcgg caccgggcgc cgctgtcccg
      301 atatttcgaa actggcaaag ctcggctata agccgcgcgt gccgctaagc gaaggcctca
      361 ggccgacgct cggctggtac cggcagaatg ccgagcgtgc gtcgaaggcc tgacatcgaa
      421 tgactgacgt cccgcgtcca agcaagatcg cgcgcgccgc aggaaccggc gccagcgttc
      481 cggttgaatg ctgccaggtg tgcgggcacg cgccgctcga aacggtgctg tcgctcggct
      541 acatgccgcc cgtcaaccag atggtgccga tcggccaggt tcagcgccag ctgccctggt
      601 ttccgaccgc gcttctgcat tgccgcaagt gcgaccttgt ccagctcggc ctcgccgtgg
      661 acccggtgat cattttcccg ccggaatatc cttacaccag cggcaccacg aaattgctgc
      721 gcgacaattt cgccgatctg cagcgcgaga gcgtggccat gctcggtctc accggcgagg
      781 acctggtcat cgacatcgga tcaaacgacg gcacgctgtt gtcgaacttc aagaacggcg
      841 gccagcgcgt gcttggtatc gagccgacgg acgtcggcga tatcgccaac cagcgcggca
      901 ttccgacgat caagcgctac ttcggactgg acacggcgcg ccaggtgaaa cgcgagcatg
      961 ggccggccag tgtcgtgacc gccgccaact gctttgcgca catggaagac gtccatgcca
     1021 ttgtcgacgg catcgtcgag atgctcaagc cggacggcgt cttcatttcc gaatcgcact
     1081 acctcgtccc gctgctcgac acacttcaat acgacacgat ctatcacgag cacctgcgct
     1141 actattcgct caccagcctc agacacctgc tggacatgca cggtctcgag gttttccacg
     1201 cgcgacccat tccgagtcac ggcggctcga ttcgcgttta tgcggcccgg cgcggcagcc
     1261 gtcccgtcca ggacagtgtt ggccgcatgc tggccgccga gccgcacggc gaagcgatgg
     1321 caaggcggct gcaggcattc cgacgcgacg tcattctctc caagcttcgc ctgctgtcga
     1381 tgctgcgcga tctgaaggaa aaaggcgcgc gcatcgccgg catcagtgca ccctcgcgcg
     1441 ccagcacgct ggtcaactac gtgggactcg acgagggatt gatcgactat gtctgcgaga
     1501 tcgcgggctc gctgaagatc ggcaaataca tgccgggaac gcaaatcccc gtcgtcgatg
     1561 agttccgcct gttcaccgac cagcccgatt gcgcggtcat cttttcctgg cacatcgccg
     1621 acgagcgtgc acccaagctc aaggccaaag gattccgcgg caaattgatc acccctctgc
     1681 ccgaaccacg cgtgctctag gtcggctcgg atgactgccg gtcacgcgca ggacaagaac
     1741 aaggaaatcg cgtttttcga tgcgcatgcc gagcatgatg cgtatgacgt tttcacgccg
     1801 caggcgaacg aacggatcat cgcggctttc atgcggctga gcggattgcc gcgcggtgcg
     1861 cgcgtggccg atcttggttg cgggtcgggt gtgtttaccg aactgcttcg gcaccagggc
     1921 tttcaaagcg tcggtctcga cataagcccc aggctcatcc agctcggacg tcgcaaatat
     1981 cccggcctcg agctgatcga aggcgacgcc gaaaacctgc cgttcgaaag cgcaagcctc
     2041 gatggcgtgc tgctgagcgg cctggtccac cattttcctg atccgcgcag gcttgccggc
     2101 gaggtcagcc gggtgctcag aagcggcggc cgttttgtcg ctttcgatcc caaccgcatg
     2161 aacccgttca tgtggctcta ccgcgaccgc tc
//
LOCUS       DASSRL010000394         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_815296, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000394 DASSRL010000000
VERSION     DASSRL010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815296"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /gene="ahcY"
                     /locus_tag="VFL49_08045"
     CDS             complement(<1..1349)
                     /gene="ahcY"
                     /locus_tag="VFL49_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7911968.1"
                     /translation="MSAVAATASSGDFHVADLSLASWGRKEILIAETEMPGLMAIREE
                     FAKSQPLKGARITGSLHMTIQTAVLIETLQALGAQVRWASCNVFSTQDHAAAAIAATG
                     TPVFAFKGESLDDYWEFTHRIFEWSDGGYSNMILDDGGDATLLLHLGTRAESDINVLA
                     KPGSEEEVCLFNSIKRYLKKDPKWYSTRLKQIKGVSEETTTGVKRLYQMAKEGKLAFP
                     AINVNDSVTKSKFDNLYGCRESLLDGIKRATDVMIAGKVAVVCGYGDVGKGSAQALRS
                     LSAQVWITEIDPICALQAAMEGYRVVTMEEAADKADIFVTTTGNFHVITHDHMKAMKN
                     NAIVCNIGHFDNEIDVASLKKYQWENIKPQVDHVIFPDGKRIILLAEGRLVNLGCATG
                     HPSYVMSSSFANQTLAQIELYVNSAKYPIGVYVLPKHLDEKVARLQLAKLGVKLTQLT
                     "
     regulatory      complement(1365..1448)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(1528..>2676)
                     /gene="metK"
                     /locus_tag="VFL49_08050"
     CDS             complement(1528..>2676)
                     /gene="metK"
                     /locus_tag="VFL49_08050"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7911969.1"
                     /translation="SEGHPDKVSDQISDAILDAILAQDKYSRVAAETLCNTGLVVLAG
                     EITSNAIVDYQSVARETIRRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDEGRGL
                     NLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQAQLRRSGQLKWLRPDATSQV
                     TIKYVDGKPKEIDTVVLSTQHHPDIEHAALAEAVIEEIVKPVLPKELIGKKINYLINP
                     TGRFVVGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKN
                     IVAAGLASKAQVQVSYAIGVAQPTSIMVTTFGTGKVSDEKLEELVARHFDLRPKGIVQ
                     MLDLLRPIYQKTAAYGHFGRSEPEFTWEQTDKAAALAADAGVKLAANA"
BASE COUNT          485 a          831 c          807 g          553 t
ORIGIN      
        1 gtcagctgcg tgagcttgac gcccagcttg gcgagctgca gacgcgccac cttctcgtcc
       61 aggtgcttcg gcagcacgta cacgccgatc gggtacttcg cggagttcac gtacagctcg
      121 atctgcgcca gcgtctgatt cgcaaacgaa gaactcatca catatgacgg atggccggtc
      181 gcgcaaccca gattcaccag tcgcccttcg gccagcagaa tgatgcgctt gccgtcaggg
      241 aaaatgacgt gatcgacctg cggcttgatg ttttcccact gatacttctt cagagaggcg
      301 acatcgatct cgttgtcgaa gtggccgatg ttgcagacga tggcgttgtt cttcatcgcc
      361 ttcatgtgat cgtgcgtgat cacatggaag ttgccggtcg tggtgacgaa gatgtcggcc
      421 ttgtccgccg cctcttccat tgtcacgacg cggtagcctt ccatcgcggc ctgcaatgcg
      481 cagatcggat cgatttcggt gatccacacc tgtgccgaga gcgaacgcag cgcctgcgcc
      541 gagcccttgc cgacgtcgcc atagccgcag accacggcaa ccttgccggc gatcatcacg
      601 tcggtggcgc gcttgatgcc gtcgagcagc gactcacggc agccgtaaag attgtcgaac
      661 ttgctcttgg tcactgagtc gttgacgttg atggccggga acgccagctt gccttccttc
      721 gccatctggt aaaggcgctt gacgccggtg gtcgtttctt cggagacgcc cttgatctgc
      781 ttcaggcgcg tcgagtacca cttggggtcc ttcttgaggt agcgcttgat cgaattgaac
      841 aggcagacct cttcctccga accaggtttc gccagcacat tgatgtccga ctctgcgcgc
      901 gtgcccagat gcagcagcaa cgtggcatcg ccgccgtcat cgaggatcat gttggaataa
      961 ccgccgtcac tccattcgaa gatgcgatgg gtgaattccc agtagtcgtc cagcgattcg
     1021 cctttgaatg cgaacaccgg cgtacccgtg gcggcgatgg cggcggcagc gtgatcctgc
     1081 gtcgagaaca cgttgcagga tgcccaacgc acctgcgcgc cgagcgcctg cagcgtctcg
     1141 atcagcactg ccgtctgaat cgtcatgtgc aacgagcccg tgatgcgggc acctttcaag
     1201 ggctgggatt tcgcgaactc ctcgcgaatc gccatgagac ccggcatttc ggtctcggca
     1261 atcagaattt ccttgcggcc ccaggaagcg agggacaggt cggcaacgtg aaaatcgccg
     1321 ctggaagcgg tagcagcaac tgcgctcatg tgaatctccg gttaaacgtc agggcgccgt
     1381 tgccattgaa ggcaatgccc gccgagcctg gcagatgcga ttcatctgct gcagcgcccc
     1441 tcggcggacg gatgcgtaaa caatcgggcg gcgggcgaag aacagactca tccgttcctg
     1501 ccgcgccgca tgtgcgttga ccgcgcgcta ggcgttggcc gccagcttga cgccggcatc
     1561 ggcagccagt gccgctgcct tgtccgtctg ttcccaggtg aactccggct ccgaacgacc
     1621 gaagtgacca tacgcggccg tcttctgata gatcggacgc agcagatcga gcatctggac
     1681 gatgcccttg ggacgcagat cgaagtgtcg ggccaccagt tcttccagct tctcgtcgga
     1741 caccttgccg gtgccgaagg tggtcaccat gatgctggtc ggctgagcca cgccgatggc
     1801 gtacgacacc tgcacctgcg ccttgctcgc caggcccgcg gcgacgatgt tcttggccac
     1861 gtaacgggcg gcataggcgg ccgaacgatc gaccttcgaa ggatccttgc cggagaacgc
     1921 gccaccgccg tgcggagccg caccgccgta agtgtcgacg atgatcttgc ggcccgtgag
     1981 accgcagtcg ccctggggac cgccgaccac gaaacgaccg gtcggattga tcagatagtt
     2041 gatcttcttg ccgatcagtt ccttcggcag aaccggcttg acgatttcct cgatgacggc
     2101 ttcggcaagc gccgcatgct cgatgtccgg atgatgctgg gtcgagagca ccaccgtgtc
     2161 gatttccttc ggtttgccgt cgacgtactt gatggtgacc tgcgatgtcg cgtcgggccg
     2221 cagccacttg agctggccgc ttctgcgcag ctgcgcctgg cgttcgacca gtcggtgcga
     2281 aagatggatg ggcagcggca tcagttccgg cgtttcatcg cacgcataac cgaacatcag
     2341 gccctgatcg cctgcaccct gatcgagatt caggccgcga ccttcgtcca cgccctgggc
     2401 gatgtccggt gactgcttgt cgtaagcgac cagcacggcg cagcccttgt aatcgatgcc
     2461 gtagtcggtg ttgtcgtaac cgatgcgccg gatcgtttcg cgcgccacgc tctggtagtc
     2521 cacgatagca ttcgacgtga tttcgcccgc cagcacgacc aggcccgtgt tgcacagcgt
     2581 ttccgccgca acacgcgagt atttgtcctg cgccaggatg gcatcgagga tggcatcgga
     2641 gatctgatcg gacaccttgt ccggatgacc ttcgga
//
LOCUS       DASSRL010000395         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_552690, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000395 DASSRL010000000
VERSION     DASSRL010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552690"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFL49_08055"
     CDS             <1..393
                     /locus_tag="VFL49_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7911970.1"
                     /translation="DLVAGRVAFTIDNLGPILPFIQAGKLIALGVSTKQPVALLPGVP
                     PIASVLKGYELSSWNVLAVPAKTPKDIVSLLSKECDRIVHIPKVAETMRSFGSEPVGG
                     TPEQTAEFLKNERPRWEAAIKAAKIPKQ"
     gene            complement(462..1595)
                     /locus_tag="VFL49_08060"
     CDS             complement(462..1595)
                     /locus_tag="VFL49_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HET7911971.1"
                     /translation="MEAEKGERTILSPNHASLRDIETPALVIDAAALQRNIEGMAAWA
                     RQTGIALRPHAKTHKSVQIARLQKKAGAAGIACATVAEAELLASAGIESLLLTAPQMG
                     EGKFARITKINSAHDIAVVVDHAAQVERLVASLGADDKPLPVLVDVDVGHSRTGIADL
                     GKGVRLAQSIAASAKLKFVGIQGFAGHAQHIRRPDERNSAADKAADILRRFAKGLAEV
                     GLDCELISGSGTGTYRRDAAGPYNELQVGSYVFMDSDYARIVDESGAGPCFEPSLFVL
                     ATTVSINISGQVTVDAGSKALATNGPPPCNFVGAPKGSSYRFAGDEHGIVTVPEGQKA
                     PALGDRLLIGATHCDPTVNLHACYHVIDASQAETWPIGARYND"
     gene            complement(1631..2632)
                     /locus_tag="VFL49_08065"
     CDS             complement(1631..2632)
                     /locus_tag="VFL49_08065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7911972.1"
                     /translation="MKYSICLATGYEGLVYPVNFCKPQDLVRHAQIAEKLGFHSVWGN
                     DHITPPRYVRDHTKGSPNFYDVLITLAMIAQATTTLRVGTALLVMPNREPVYLAKQVA
                     TLDQMSDGRFIMAIGIGAYREEFEAWVGNRYEQPHRGDMMEEGISIFKSLTTEKISSF
                     DGKYYSYKDIEMFPKPKQNPFPLYIGGHNMKAVERAATIGYGWLPGWRPLHEIEERIQ
                     ALHKYANETGRNPKEIEIAPQFSLLIGKTQEAAEAIYMKSGLVAHRVSLAHTGRDPVH
                     QVTANLVGSPQAIIDKIYKLKAMGVDHCAAMAVAVNTQEELIEQLHWFSEEIIPHID"
     gene            complement(2684..>3463)
                     /locus_tag="VFL49_08070"
     CDS             complement(2684..>3463)
                     /locus_tag="VFL49_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7911973.1"
                     /translation="DEMGITPQCMDQLQSALPEAKFIRCFSLFERTRSIKTQGEIEIL
                     RKAAQATERAIDAALKVAAEGITEREMLREFNACLARNDTAPVVGCIGFGNRSAMINV
                     QPSDRRLKRGDLIRFDVGGRYRHYRSDMSRGATLGEPTAKISKYYRAVEKGVLRGYDI
                     IKPGLKVADLFKDIVATVQREGIPHFKRSHVGHGIGVDGYDPPNIAESSADIVEENMV
                     VCIETPYYELGFAGLQVEDMVRVTKDGAESLMTLPTALRVL"
BASE COUNT          701 a         1037 c          964 g          761 t
ORIGIN      
        1 gatctggtcg ccgggcgcgt cgcgttcacg atcgacaatc tcggccccat tctgccgttc
       61 atccaggcgg gcaaactcat cgcgcttggc gtttcaacga aacagccagt cgctttgctg
      121 cccggcgtgc cgccaatcgc atccgttctg aaaggttacg aactgagctc gtggaatgtc
      181 ctcgccgtgc ctgcgaaaac gccaaaggac atcgtcagcc tcctcagcaa agagtgcgat
      241 cgaatcgtcc acattccgaa ggtggcggaa accatgcgct cattcggctc ggagcccgtc
      301 ggcggcacgc cggaacaaac ggcggaattt cttaaaaacg aacgcccgcg ctgggaggcg
      361 gctatcaagg cggcaaaaat tccgaagcaa taggaagccc tgaagcgcca agccggcgcc
      421 gcggcgccgg cttttttact gctctgcgga tcgtgtgccg atcaatcgtt gtagcgtgcg
      481 cctattggcc acgtttcagc ctggctggca tcaatgacgt gatagcaggc gtgcaggttg
      541 acggtcggat cgcaatgcgt tgctccgata agaaggcgat cgccgagggc cggcgctttt
      601 tgcccttccg gaacggtgac aattccgtgc tcgtcacctg caaaccggta actgctgccc
      661 ttcggggcgc caacaaaatt gcaaggcggc ggcccgttgg ttgctagcgc cttgctgccg
      721 gcatcgaccg tgacctgacc ggaaatattg atcgacacag tcgtcgcaag cacaaagagg
      781 cttggctcga agcaaggtcc ggcgccgctc tcatcaacaa ttcgggcgta atcagaatcc
      841 atgaagacat acgagccgac ctgaagttcg ttgtagggtc ctgcggcgtc tctccgatag
      901 gtcccagtgc cgctcccgct gatcaactcg cagtcgagtc ccacctctgc cagccccttg
      961 gcgaaccgcc gcaaaatatc ggcggccttg tccgccgcag aattccgttc gtccgggcgc
     1021 cgaatatgtt gcgcatgacc agcgaaaccc tggatcccga caaatttcag tttagccgac
     1081 gcagcaattg attgcgcaag gcgaacgccc ttgccgaggt cggcaatgcc ggttcgcgag
     1141 tggccgacat cgacgtcaac gagcacgggc agcggtttgt cgtccgcgcc gagacttgca
     1201 acgagccgct caacctgcgc ggcgtggtca acaaccacag caatatcgtg cgcactgttg
     1261 atcttcgtta tgcgggcgaa cttgccttcg cccatttgcg gagccgtcag cagcaggctc
     1321 tcgattccgg ccgaagcaag caattcagct tccgcaaccg tggcgcaggc gatgcctgct
     1381 gctcctgctt ttttttggag cctggctatt tgcacggatt tgtgtgtctt tgcgtgcggc
     1441 cgcaaagcga tgcccgtctg cctcgcccag gcagccatgc cctcaatgtt ccgttgcaag
     1501 gcagcggcat cgatgacgag cgccggtgtc tcaatatcgc gcagagaagc gtggttgggc
     1561 gaaagtattg tgcgctcccc tttttctgct tccactgcaa tcgctggaac gcgcgtccgc
     1621 gtaatggcag ctagtcgata tgcgggatga tctcttcaga gaaccaatgc aactgctcga
     1681 tcaactcttc ctgcgtattc accgcaaccg ccatcgcggc gcaatgatca acgcccattg
     1741 ccttgagctt gtaaatttta tcaatgatcg cctgcggcga cccgacgaga ttggcggtga
     1801 cctggtgcac cgggtcgcgg ccggtatgcg caagcgacac acgatgcgcg accagtccac
     1861 tcttcatata tatagcttcc gccgcctcct gggttttgcc gatcaatagc gaaaattgcg
     1921 gagcgatctc gatttccttg ggatttcttc ccgtttcgtt cgcatattta tggagcgcct
     1981 gaatgcgttc ttctatctcg tgcaacggcc tccaccccgg caaccagccg tagccgatcg
     2041 tggcggcacg ctcgacggcc ttcatattgt gcccgccgat ataaagcgga aaaggattct
     2101 gcttcggttt cggaaacatc tcgatatctt tatacgagta gtattttccg tcgaaggagg
     2161 agattttttc cgtcgtcaga ctcttaaaaa tactgatccc ctcctccatc atgtcgccgc
     2221 gatgcggctg ctcgtagcga ttcccaaccc aagcttcgaa ctcttcgcga taggcgccga
     2281 tcccgatcgc catgatgaac cgcccgtcag acatttggtc gagagtagcc acctgcttgg
     2341 cgagatagac cggctcgcgg ttgggcatga ccaacaatgc cgtcccgaca cgcagcgtgg
     2401 tagtggcctg cgcgatcatc gcgagcgtaa taaggacgtc gtagaagttt ggagagccct
     2461 ttgtgtgatc gcggacgtag cgggggggcg taatatgatc gttgccccag accgaatgaa
     2521 acccgagctt ttccgcgatc tgcgcgtgac ggaccagatc ctgcggcttg cagaagttta
     2581 ccggatagac aaggccttca tatccggtcg ccagacagat ggagtatttc atcggtgtcg
     2641 atctccggca gtcgcgatac gcctttccac ttggcggatc gcgttagagc acgcgcaagg
     2701 cggttggcag ggtcatcagc gattcggcac cgtctttcgt aacgcgaacc atgtcttcga
     2761 cctgcaatcc agcaaagccg agctcgtagt aaggcgtttc gatacacacg accatgttct
     2821 cttccacgat gtctgccgag ctttcggcga tattcggagg gtcgtagccg tcgacaccga
     2881 tgccatggcc aacatggctt cgcttgaaat gtggaattcc ctcgcgctga acagtcgcta
     2941 cgatgtcctt gaacagatcg gcgaccttga gcccaggctt gataatgtca tagccgcgca
     3001 aaacgccttt ttcgacggcg cggtaatatt tgctgatttt cgccgtcggc tcacccaagg
     3061 tcgccccgcg cgacatatcg gaccgatagt gacggtagcg tccgccaacg tcgaaacgaa
     3121 tcaaatcgcc ccgtttgagc ctgcgatcgg acggctgcac gttgatcatt gcgctgcgat
     3181 tgccgaagcc gatgcatccg acgaccgggg cagtatcgtt ccgcgcaagg catgcattga
     3241 attcgcgaag catttcgcgc tcggtgatgc cttcggccgc aaccttgagg gccgcatcga
     3301 tagcgcgctc ggtcgcctgc gctgcctttc gcaaaatttc gatctcgccc tgcgttttaa
     3361 ttgaacgcgt tctttcgaaa agcgaaaaac accggatgaa cttcgcctca ggcaaagcgg
     3421 actgcagctg gtccatgcat tgtggtgtga tccccatctc gtc
//
LOCUS       DASSRL010000396         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_448149, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000396 DASSRL010000000
VERSION     DASSRL010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448149"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /gene="asnB"
                     /locus_tag="VFL49_08075"
     CDS             <1..1948
                     /gene="asnB"
                     /locus_tag="VFL49_08075"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET7911974.1"
                     /translation="MCGIAGIFDPAGRGTADLRQRLAPMIELLAHRGPDADGLWIDAE
                     AGTGLGHRRLSIVDVTSAGAQPMASADGRWITTYNGELYNTADLRAEVERVNPQVRWR
                     GHSDTEVLLEAVSLWGVAGAIRRCNGMFALAFWDRRERRLWLVRDRIGIKPLYWARLP
                     GGGLLFGSELRALRSYPGFKTRIDTKAVSAYLRSACIPAPHTIYEETYKLPPGHLLSA
                     GAGQEPKLSCYWDLRQIAVDGQRRIDARNHEELADGLETLLADSVVRQMVSDVPLGAL
                     LSGGIDSSLVVALMQAKSSRAVRTFTIGFRDRRYNEADDARRVAQHLGTDHTELVVEP
                     AVAQAVIARLPDIYDEPFADSSQIPTFLVSELARRSVTVALSGDGGDEGFGGYVRHHW
                     INRLSAWNRIVPGPLSRTLGGGLRLLSPAAWDALLSPLPARLRPTFVGDKIYKAAALA
                     ALGGTGQIYRRMIAQWPEPSRALRQANEPSAVWDDPAIADDLADPSARTRYFDMMHYL
                     PDDILTKVDRASMAVSLEARVPLLDHRVVEYSWRLPRSALIEGSKGKIILRRILHKYV
                     PRTLVERPKAGFAIPIGDWIRGPLSDWAAELLSESSLAKRGLFDVAEIRRVFAEHQAG
                     IRDWQYPLWTILMFQAWHNRWAAR"
     gene            1945..3084
                     /locus_tag="VFL49_08080"
     CDS             1945..3084
                     /locus_tag="VFL49_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7911975.1"
                     /translation="MSPASVPRLLYVVSEDWYFLSHRLPMARAARAAGYEVHVATRVV
                     DGAKAIEAEGFRLHAIPFARGGVGPLAALRTIAALRRIHRNVRPVLAHHVALQAAVFG
                     SLAAIGRRTTCVNALTGFGFTFISENIKARLLKPLIVFLLRFLCMRGNNVALVQNPDD
                     RNALLSLGIAADHIALIPGSGVDVERLQPSAEPAGTPTVAFVGRLLEDKGIRPLVAAI
                     RQLQERGTAINLLIAGDMDPANPSSVKAAELHEWTRSPGITWAGHVNDISEIWAKSHI
                     AALPSRREGLPKALLEAAACGRPMVATDVPGCREVVIEGKTGLLVRLDDRNALADAIG
                     KLAASASLRARYGAAARILAVEKFSAAAIGRQTMQLYDRLLASSR"
     gene            3203..4471
                     /locus_tag="VFL49_08085"
     CDS             3203..4471
                     /locus_tag="VFL49_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911976.1"
                     /translation="MHGLLALAKKARYRPRGKIFYVCYGTHHNDAIYAENLLEFFREE
                     GTPAEAIRLQPQNPQRELLRCLNDSTLGVLGLNVQLDHSWIDSDNFLDLAAAADIPVI
                     HWFLDHASSRWREFTNANAQNSRFLFLSPLAERYFLQYALPGCLTACTVNTGQNHRSR
                     IAQLTRDEFLSREIACLVPLNLRRISGSLADAMLRQQALDRKLARAVDRACEQAYYDL
                     DGALERHLQNALADAGLAVNNSLFNDAFQIVEDVVQIRRRQRIFEIARAFPVLIQSDE
                     SAAPFASGGKARFETNVSMRTTFARMKSARAVLSVSRVNDELHNRIQNGVNAGCANIV
                     EDNTVNRSVLTDGKNALLFNYRDDSLREHLEFVCSNPRRAYELAQTGFVLRDETPFRF
                     GGFHNILELAQTPVPGVRHPELARQPSLAL"
BASE COUNT          812 a         1467 c         1405 g          908 t
ORIGIN      
        1 catgtgcgga attgccggaa tcttcgatcc cgccgggcgc gggaccgccg atttaaggca
       61 gcggcttgcg ccgatgatcg agttgttggc gcatcgcggc ccggatgccg acggcctgtg
      121 gatcgatgcc gaagccggca ccgggcttgg gcaccggagg ctctcgatcg tcgatgtcac
      181 atcggccggt gcgcagccaa tggcatcagc cgacgggcgc tggatcacca cctataacgg
      241 agagctttac aacaccgccg atttgcgggc tgaggttgag cgcgtaaatc cgcaggtgcg
      301 ctggcgcggg cattcggaca ccgaagttct gctcgaagcc gtgtcgctct ggggtgtcgc
      361 cggggcgatc aggcgctgca acggcatgtt cgcgctggca ttctgggatc gccgcgaacg
      421 gcgcctgtgg ctggtgcgcg accgcatcgg tatcaagccc ctctattggg ccaggctgcc
      481 cggcggcggc ctcctgttcg gctccgaact gcgcgcgctc cgcagctatc ccggattcaa
      541 aacgcggatc gataccaagg ccgtcagcgc ctatctgcgg tcggcgtgca tccctgcccc
      601 tcacacgatc tacgaagaga cgtacaagct cccaccgggc catctgctga gtgccggggc
      661 gggccaggag ccgaagctct cctgctactg ggacttgcgc cagatcgccg tcgacggcca
      721 gcgccgcatt gacgcccgca accacgaaga gctcgcggac gggctggaga cgttgcttgc
      781 cgattcagtc gtacggcaaa tggtgtctga cgtgccattg ggcgcgctgc tgtccggcgg
      841 catcgattca tcgctcgtcg tcgcgctgat gcaggcaaag tcatcgaggg ccgtccgtac
      901 gttcaccatc ggatttcgcg acaggcgcta caacgaggcg gacgatgcgc ggcgcgtcgc
      961 gcagcatctc ggaaccgacc ataccgaact tgtcgtcgag ccggcggtcg cgcaggccgt
     1021 gattgcgcgg ctgcccgaca tctacgacga gccgttcgcc gattcctcgc aaatccccac
     1081 cttcctggtt tccgagctgg cgcgccggtc ggtcacggtt gcgctgtccg gcgacggtgg
     1141 cgacgaaggc ttcggcggat atgtgcgcca ccattggatc aaccggctga gtgcctggaa
     1201 tcgaatcgtg ccggggccac tatcgcgcac gcttggcggc ggcctgcgat tgctctcgcc
     1261 ggcggcctgg gacgcacttt tgagcccgct gccggcgcgg ctgcggccga cctttgtcgg
     1321 tgacaaaatt tacaaggccg ctgcgctcgc ggcgctgggg gggacagggc agatctaccg
     1381 gcgaatgatc gcgcaatggc ccgagccttc gcgcgcgttg cggcaagcga acgaaccgtc
     1441 cgcggtctgg gacgacccgg ccatcgcaga cgatctcgcg gacccgagcg cccggacgcg
     1501 ttacttcgac atgatgcatt acctgcccga cgacattctg accaaggtcg atcgggccag
     1561 catggccgtt tcgctggaag cgcgcgtgcc gctgctcgat catcgcgtcg tggaatattc
     1621 gtggcgcctt ccgcgctccg cgctgatcga gggctcgaaa ggcaaaatca tcctgcggcg
     1681 tatcctccac aaatacgtgc ccaggaccct ggttgaacgg ccgaaagccg gattcgccat
     1741 tccgatcggc gactggatcc gcgggcccct gtcggactgg gccgccgaac tgctgtccga
     1801 aagctcgctt gcaaagcgcg ggctcttcga tgtcgccgag atccggcgcg tcttcgccga
     1861 acaccaggcc ggcattcgcg actggcaata cccgctgtgg acgattctga tgtttcaggc
     1921 gtggcacaac cgctgggccg cccgatgagc cccgcaagcg tccctcgcct tctctacgtc
     1981 gtgtcggagg actggtattt cctgtcgcac cggcttccga tggcccgcgc ggcccgtgct
     2041 gccggctacg aggtgcacgt cgcaacgcgc gtcgtcgacg gcgccaaagc aatcgaagcc
     2101 gagggcttca ggctgcacgc cattccgttt gcacgcggcg gcgtcggacc tctcgcggcc
     2161 cttcgcacca ttgccgcctt gcgccgcatt catcgcaatg ttcgtccggt cctcgcccat
     2221 catgtcgctt tgcaggcagc ggtgttcgga tcgcttgccg ccatcggccg ccgcaccacg
     2281 tgtgtgaacg ccctgaccgg tttcggcttt acgttcattt ctgaaaacat caaagcgcgg
     2341 ctgctgaaac cgctgatcgt ctttctgctg cgattccttt gcatgcgcgg aaataacgtc
     2401 gcgctggtgc agaacccgga cgaccgcaac gccttgttgt ctctcgggat tgccgccgat
     2461 cacatcgcgc tcattcccgg ctcgggcgtc gatgtcgaac gcctgcagcc gtcggccgag
     2521 ccagcaggaa cgccgaccgt cgcctttgtc ggacgcctgc tcgaagacaa aggcatacgc
     2581 ccgcttgttg ccgcgatccg ccagctgcag gagcgcggca ccgccatcaa cctgctcatc
     2641 gctggcgaca tggatccggc caatcccagc tcggtgaaag ccgccgagct ccacgaatgg
     2701 acaaggtccc cgggcattac ctgggccggc catgtcaacg acatatcgga aatctgggca
     2761 aagtcgcaca tcgccgccct tccgtcccgt cgcgagggat tgccgaaagc gttgctcgaa
     2821 gccgctgcgt gcggccgccc gatggttgca accgatgtgc cgggctgccg ggaggttgtg
     2881 atcgaaggca aaacgggact tctcgtccgt ttggacgata ggaacgcatt ggccgatgcg
     2941 atagggaagc tcgcagcatc ggcgagcctg cgggcgcgtt atggtgccgc cgcgcggatc
     3001 cttgcagtcg aaaaattctc agcagcagcg atcggccggc aaaccatgca actttacgac
     3061 cgtctcctcg ccagcagccg ctaattgcgt cagaacctgc gacgcttcgc catgggcata
     3121 gagttggtcg gcaacggcgg cttgcgctat agtggattat tcggctgaaa agacattgtt
     3181 ctgtttgtga ttggtagcgc gtatgcacgg tttattagct ctcgcgaaaa aggcccgata
     3241 tcgcccgcgc ggtaagattt tttatgtttg ctacgggacg catcataatg acgccatcta
     3301 cgcagaaaat ctgctggaat tctttcgtga ggaaggcacg cccgctgagg ccataagatt
     3361 gcagccgcaa aatccgcaac gcgagctgct tcgctgcctg aacgattcaa cactcggcgt
     3421 cctcggcctg aatgttcaac tcgatcactc gtggatcgat tccgacaatt ttctcgacct
     3481 tgccgccgcg gccgacatac ccgtgattca ctggtttctt gaccacgcca gctcaagatg
     3541 gcgcgagttc acaaatgcga acgcgcaaaa ctcgcggttt ctttttctgt ctcccttggc
     3601 ggagcggtat tttctgcaat acgctttgcc cggctgtctt accgcctgca ccgtcaatac
     3661 cggccaaaac caccgctcgc gcatcgcaca actcacccgc gatgaatttc tgtcgcgcga
     3721 aatcgcgtgt cttgttccgc tcaacctgcg gcgcatcagc ggctctcttg cggatgcgat
     3781 gctgcggcag caggcgctcg acaggaagct cgcgcgggcc gtggatcgtg catgcgaaca
     3841 ggcttattac gacctggacg gcgcgctcga gcgtcatctg cagaacgcgc tcgccgatgc
     3901 cggccttgct gtgaacaaca gtctcttcaa cgacgcgttc cagatcgtcg aggacgtcgt
     3961 ccagatccgg cggcgccaaa ggattttcga gatcgcgcgc gcgtttccgg tcttgatcca
     4021 atccgacgaa tcggcggcgc ccttcgcctc cggcggaaag gcgagattcg aaaccaacgt
     4081 cagcatgcgg accacattcg cgcgaatgaa atcggcccgc gctgtgttga gcgtaagccg
     4141 ggtcaatgac gagcttcaca atcggattca gaacggcgtg aacgcgggct gcgccaacat
     4201 cgtcgaggac aataccgtaa accggtccgt tctgacggat gggaagaacg cgttactttt
     4261 caactatcgg gacgactcgt tgcgcgagca cctggaattc gtgtgcagca atccgcggcg
     4321 ggcctacgag ttggcgcaaa ccggatttgt gctccgcgat gaaacgccat tccggttcgg
     4381 cggttttcac aacattcttg agttggcgca gacgcccgtc ccgggcgtca gacacccgga
     4441 actcgcacga cagccgtcgc tcgcgctata atgccggaat gtccgatttc tggcttgtgg
     4501 ccggccagct tgcattgatt gctgctgttg ccggtgttgt cagttatggc cttattgtca
     4561 tcctgggcgc ctcgctcgcc aggtatgcat tg
//
LOCUS       DASSRL010000397         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1532970, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000397 DASSRL010000000
VERSION     DASSRL010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532970"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFL49_08090"
     CDS             <1..590
                     /locus_tag="VFL49_08090"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET7911977.1"
                     /translation="SAGDQPDAKAVFDLDPRLLLDTARRLRDAGELPTGRKVGGNFNF
                     FLGSADNPIDPPPGWKPTGLQAKIDAGAQFVQTQFCMDAGVVHRYMARLAEQGLTDRL
                     SFLIGVVPLRSAKSARWIKEKLFGAVIPDALIGRLERARDPVAEGASICTDIIRDLSG
                     VPGVAGVHIMAPGNDAAVPEIIRTARADIGRLAPA"
     gene            706..1689
                     /locus_tag="VFL49_08095"
     CDS             706..1689
                     /locus_tag="VFL49_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911978.1"
                     /translation="MKTRMIGLSAIAAVAVLAAFVAPGAAQEKVWKHGLINAKADAGI
                     FLMVSTRDFAKKQGLKIEISQFKDDQLALKALIAGELDSFEGGPQGVFAADAKGADVK
                     LLGCHWIVVPHGIYANEKITKAQDLKGKQIAVSAPNSMPDMLARSALAKFGISDNEVK
                     LAAVGGDKDRYQALIGGVVDAAVVSNEYQPIAPKNIHLLVAGREAVPNFLRVCMTSSA
                     KKLAERGDDAVKFLAAEMSALRFALANRDATIALTREIIHAKPDDPRPAFVYDDAIQH
                     HAVDPTLPLPADKIQWIQEQMVKAGKLKAPLDLKTVTAPEYRERALKVIGH"
     gene            1712..2491
                     /locus_tag="VFL49_08100"
     CDS             1712..2491
                     /locus_tag="VFL49_08100"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911979.1"
                     /translation="MGEVSFLDVSKRFTLRVDSEVHHVQALDRVSFTVKDGEIVALIG
                     PSGCGKTTALRVAMGLDPSTGGRVTVDGREIDGCGHDRGMVFQHAELLPWLSALQNVM
                     FGLEMKGIRGTQLRETAEKYLHLVGLKESQNRRPHQLSGGMKQRVGIARALAIDPKVL
                     LMDEPFGALDAQTRETLQAELLDIHARTGKTILFVTHDLDEAVIIADRIVVMKNGRVQ
                     EIMNVPLKRPRPDLSLVRGMPEFADTRYKVWRALHAGTTVH"
     gene            2491..3318
                     /locus_tag="VFL49_08105"
     CDS             2491..3318
                     /locus_tag="VFL49_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7911980.1"
                     /translation="MVDIADRQENATAKEEAAERKPLPNWMITVASVAVLLIVWEIFG
                     RRINPVFGSYPSAIAEAFWELVISGQLWSALYESLRPFVIGYALAILIGVPLGLFIGG
                     FRAAEAALGLYITAGYAMPLVALVPLLILWLGLGFAVKVAVIFLMSLFPICINTWLGV
                     TAVPKTLIEVGKSFVAPDTVILRRIILPATLPYIMAGIRLAVGRAVVGMVIAEFFTTI
                     SGLGAVIINSANNFDTATMFVPIIILMVLAIGLNWLIGFVERWVAPWQAEISGRDRE"
     gene            complement(3331..4266)
                     /locus_tag="VFL49_08110"
     CDS             complement(3331..4266)
                     /locus_tag="VFL49_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7911981.1"
                     /translation="MIVITGFLGAGKTTLLKRFLGTAQGQGTAVVVNEFGATGIDDAL
                     VRPSADETVLLGNGCLCCITRTDLQQALRRMIIERERGELPDFKRVVIETSGLADPSP
                     ILQTFATDRALGGVFHVEAVVTVVDAVTGGDTLGWSAEARKQAILADRLIISKTDIAG
                     EDAGAALSATLRALNPGAEIFEAVNGDIDPACLTKPASDQRNTFVAEAAHSDGIGSFV
                     LTENAPLAWSVFAKTMDALMQLRGPDLLRVKGFLNVKDCRGPVVVQFVQHLAHPPVEL
                     QSWPDETRQSRVVFITRNISEQQVRDLLEALRKLV"
     gene            complement(4341..5576)
                     /locus_tag="VFL49_08115"
     CDS             complement(4341..5576)
                     /locus_tag="VFL49_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911982.1"
                     /translation="MSAANNPLNKSGEPVTWNFKLLAQNDLGGFGGMGEGMSVQLAKD
                     GRRIIWLAHESAPKNFTGVDVSDPRNPKVVVQTDLPKPYMRSNSLELSGDIMAVAYQT
                     QKVNQQPAGLELFDLSVPEKPRSISFLDCSGANSRGVHQLWFCDGEYVHMASGAPDFE
                     PSHPLDDQFYRCIDVRNPSKPVEVGRWWMPGTKKGETPLPRHKLDKGYRAHNTNVYKQ
                     RKDRLYLCYIDGGMFVMDIADKSKPKPISHWTNSPPYTGFMHTALPLFDRGLLIVTDE
                     STENNAEDWPKLVWVVDARDETNLVSIATCPLPPVDAYAGRGGRFGAHNIHENPPVPT
                     AWQSDQIVVGTFFNGGLRAYDISNPYQPKEVGTFVPPPPKGTPAGAVQLNDVFVDERQ
                     IVYTVDRHTGGLYTLEMDF"
     gene            complement(5658..6647)
                     /locus_tag="VFL49_08120"
     CDS             complement(5658..6647)
                     /locus_tag="VFL49_08120"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7911983.1"
                     /translation="MTISNKGITRRHALAATGAGVLAAGFGVRPALALETVRQGYQTN
                     MWGMPTYYLLRSGLLEKHGVKFEEFAVPSGNLTMQQMVARQVDMGTYAGPSFAIGHDK
                     GGLVAIAMIEYVGKTARVLARKDLGITKVEQLKGKKVANQTGSSTGNIFVDQIAPKAG
                     LQKGDYQEIRMNVNDMVAAMAAKTVDAMVNVEPYNAIAEAEGLGVTISEFYDFDKLPV
                     FMAATPEFVDKSPDTVVAYLKAWLEVAKDFKSSPGKVADTIYSFYTSKGYTMSKDTFS
                     KALARVEVQPGWPTDLIPYMNHHSEILVKANKLKAIPDWSKAFRTDFLKKAMA"
     gene            complement(6660..>7316)
                     /locus_tag="VFL49_08125"
     CDS             complement(6660..>7316)
                     /locus_tag="VFL49_08125"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7911984.1"
                     /translation="GKSTLLNIIAGFLSPAAGEIRIGGKPVTGSGMDRGVVFQDFAQL
                     FPWRTALGNVAFGLEMKGVPKEERNRIAREQLRLVKLEKFTGSYPHHLSGGMQQRVAI
                     ARALAYNPSVLLMDEPFAALDALTRDDMQRLLADVWRETRKTVIYVTHNVAEAVYLAD
                     RVIVMSPHPGTVKTEMKIDLPRPRDPLSVEFVECQKELLRHLGHDAGETKAMNGKARS
                     "
BASE COUNT         1368 a         2173 c         2288 g         1487 t
ORIGIN      
        1 cgagcgccgg cgatcaaccg gatgccaagg cagtctttga tctcgacccg cggttgctgc
       61 tcgataccgc acggcgcctg cgcgacgccg gcgagcttcc caccggccgc aaagtcggcg
      121 gcaacttcaa tttttttctg ggcagtgccg acaatccgat tgacccgccc cccggttgga
      181 aaccgacggg gctgcaggcg aagatcgacg ccggcgccca attcgtgcag acgcagtttt
      241 gcatggatgc aggagtggtg catcgctata tggcgcggct tgctgagcag ggtctgaccg
      301 acagattgtc attcctgatc ggcgtcgtgc cgctgcgttc ggcaaagtcg gcgcgttgga
      361 tcaaggagaa gttgttcggc gccgtcatac ccgatgcgct gatcggccgg ctagagcgag
      421 cgcgggatcc ggtagcggaa ggcgccagca tctgcaccga catcatacgc gacttgagcg
      481 gcgtgccggg cgttgcgggc gtgcacatca tggcgccggg gaatgatgca gctgtgcctg
      541 agatcataag aaccgcacgt gcggatattg ggcgccttgc gccggcgtga ccgcttttgg
      601 cggggcccga ttgccatcgc gccaccggcc caaatgatgc tagaattccg cgggaacaac
      661 gcggcacaga cgccatcaag gcgcggtttc agggaggata aacgaatgaa aacccgaatg
      721 attgggcttt ccgccatcgc ggccgttgcg gtcttggccg ctttcgtcgc gcccggcgcc
      781 gcgcaggaaa aggtctggaa gcatggcctt atcaatgcga aagctgacgc cggtattttc
      841 ctgatggtgt cgacgcgcga ttttgcaaag aagcaaggcc tcaagattga aatctcgcaa
      901 ttcaaggacg atcagctggc gcttaaagcg ttgattgcgg gcgagctcga cagcttcgaa
      961 ggcggaccgc agggcgtgtt tgcggccgat gcaaagggtg ccgatgtcaa gctgctcgga
     1021 tgccactgga ttgtggtgcc gcacggcatc tatgccaacg aaaagatcac gaaggctcag
     1081 gatttgaagg gcaagcagat tgcggtgtca gcccccaatt caatgcccga catgctggcg
     1141 cgctcggcgc ttgcaaaatt cggcatctcc gacaacgaag tgaagctcgc cgccgtcggc
     1201 ggcgacaaag accgctatca ggctctgatt ggcggcgtgg tggacgccgc agtcgtatcg
     1261 aacgaatacc agccaatcgc cccgaagaat atccatctgc tggtggcggg tcgcgaagcc
     1321 gtgccgaatt tcctgcgggt atgcatgact tccagtgcca agaaactggc cgagcgtggt
     1381 gacgatgccg tcaagttcct tgctgcggaa atgagcgcgc tgcgctttgc gctggcgaac
     1441 cgagacgcca cgattgccct gacgcgtgag atcattcatg ccaagccgga cgacccgcgg
     1501 ccggcctttg tttatgatga tgccattcag catcatgccg tcgatccgac gctgccgctg
     1561 ccggcggaca agatccagtg gatccaggag cagatggtga aggccggcaa gctgaaggcg
     1621 ccgctcgatc tgaagaccgt caccgcgccc gagtatcgcg agcgggccct gaaggtcatc
     1681 ggccactaac cagtacggtc cggagttcgg aatgggtgag gtgagtttcc tcgacgtctc
     1741 caagcgtttc acgctgcgcg tggacagtga ggtccatcat gtgcaggcgc ttgaccgggt
     1801 cagcttcacg gtcaaggacg gcgagatcgt cgccttgatc ggcccctcgg gctgcggcaa
     1861 gacgaccgcg cttcgcgttg cgatgggact cgatccatcg accggggggc gcgtcaccgt
     1921 ggatggtcga gagatcgacg ggtgcggcca cgaccgcggc atggtgttcc agcacgcgga
     1981 gctgctgccc tggctttctg ccctgcagaa cgtgatgttc gggcttgaaa tgaaaggcat
     2041 tcgtggcacg caactgcgtg aaacagccga aaaatacctg catctcgtag gtctgaagga
     2101 atcgcagaac cggcgtccgc accagctttc gggcggaatg aaacagcgcg tcggcattgc
     2161 gcgtgcgctg gccatcgacc ccaaggtgct gttgatggat gagccattcg gcgccctcga
     2221 tgcccagact cgtgaaacac tacaggccga attgctcgac atccacgcgc gcaccgggaa
     2281 gaccattctg tttgtcaccc atgatctcga tgaggcggtg atcatcgccg atcgcatcgt
     2341 ggtgatgaag aacggccgcg tgcaggagat catgaacgtc ccgctcaagc gcccacgccc
     2401 ggacctcagc ctcgtccgcg gcatgcccga atttgcggac acccgataca aagtctggcg
     2461 tgcgctgcac gccggcacca cggtgcactg atggttgata tcgccgacag gcaggaaaat
     2521 gcgacggcga aagaggaagc cgccgaacga aagccgctgc cgaactggat gatcacggtc
     2581 gcgtcggtcg cggtgctgct gattgtctgg gagatattcg gccgacgcat caacccggtg
     2641 ttcggttcct atccgtcggc aatcgccgaa gcgttctggg agctcgtgat tagcgggcag
     2701 ctatggtcgg cgctttacga atccttgcgg cccttcgtga tcggctacgc tcttgccatt
     2761 ctgattggcg tgccgcttgg gctctttatt ggcggttttc gcgcggcaga agcggcgctt
     2821 ggcctgtaca tcacggccgg ctacgccatg ccgctcgtgg cattggtgcc gttgctgatc
     2881 ctgtggctcg gacttggatt tgccgtcaag gtcgcggtca tattcctgat gtcgctgttc
     2941 ccgatctgca taaacacatg gcttggcgtc accgccgtcc cgaaaacgct gatcgaagtc
     3001 ggaaaatcct tcgttgcgcc tgacaccgtc atcctgcgcc gcattatcct gccggcgaca
     3061 ctgccttaca tcatggccgg cattcggctg gcggtgggac gtgcggtggt cggcatggtg
     3121 attgcggagt tcttcacgac catttccggg ctcggcgcgg tgatcatcaa ttctgccaac
     3181 aatttcgaca ctgcgacgat gtttgttccg atcatcattc tgatggtgtt ggcgattggg
     3241 ctgaactggc tgatcggctt cgtcgagcgc tgggtggcgc cctggcaggc cgaaatttcc
     3301 ggccgggatc gggagtaggc cgcagactcg ctaaaccagc ttgcgcaaag cttccagcag
     3361 gtcgcgcacc tgctgctccg aaatattgcg cgtgatgaaa acgacgcggc tctggcgggt
     3421 ctcatccggc cagctctgca gctcgaccgg cgggtgggcc aggtgctgca cgaactgcac
     3481 gacgaccgga cccctgcagt ctttcacatt gaggaagccc ttgacgcgca gaaggtcggg
     3541 cccgcgcagc tgcatcaatg catccatggt cttcgcaaaa acggaccagg ccagcggtgc
     3601 attttcagtc aaaacgaagc ttccaatgcc gtcgctgtgc gcggcctccg caacaaaggt
     3661 attgcgctga tcggaggccg gctttgtgag acaggcagga tcgatatcgc cgttgaccgc
     3721 ttcgaagatt tccgcgccgg gattaagcgc gcgcaatgtc gccgacagtg ccgcgccggc
     3781 gtcttcgcct gcaatgtccg tcttgctgat gatgagccgg tcggcaagga tcgcctgctt
     3841 gcgcgcttca gccgaccagc caagcgtatc gcccccggtg acggcatcca caaccgtcac
     3901 gaccgcttcg acgtgaaaca cgccgcccag cgcgcggtcg gtcgcgaaag tttgcaggat
     3961 cgggctcggg tcggcgagcc cgctggtctc gatcacaacg cgtttgaaat caggcagttc
     4021 gccgcgctcg cgctcgatga tcatccggcg caacgcctgc tgcagatcgg tgcgggtgat
     4081 gcagcacagg cagccattgc cgagcaggac ggtctcgtcg gcgctcggcc gcaccagcgc
     4141 atcgtcgatg cccgttgcgc cgaactcgtt gacgacaact gcggtacctt gcccttgggc
     4201 cgttcccaga aagcgcttga gtaacgtggt ctttccagca ccgagaaagc cggtaatcac
     4261 gatcaccgga aaccgcgctc cacgttcgcg cctgagtgca cggccgaatt gcccggcggc
     4321 gggcgagggg aagagttcgt tcagaaatcc atctccagcg tgtacaggcc gccggtgtgg
     4381 cggtcgacgg tgtagacgat ctgccgttcg tccacgaaca catcgttgag ctgcaccgcg
     4441 ccggctggcg tgcctttagg cggcggcgga acgaaggtgc cgacttcctt gggctggtag
     4501 ggattggaaa tatcataggc gcgcaggcca ccgttgaaga atgttccgac gacgatctgg
     4561 tcggattgcc aggccgtcgg caccggcgga ttttcatgga tgttgtgcgc gccgaagcgg
     4621 ccgccgcggc cggcataggc gtcgaccggc ggcagcgggc aggtggcgat ggagacgagg
     4681 ttcgtctcgt cgcgtgcgtc gacgacccac accagcttcg gccaatcctc ggcgttgttt
     4741 tcggtcgact cgtcggtgac gatcagcagc ccacggtcga acagcggcaa tgcggtatgc
     4801 atgaaaccgg tatagggcgg cgaattcgtc cagtgtgaaa tcggcttcgg cttcgacttg
     4861 tcggcgatgt ccatcacgaa catgccgccg tcgatgtagc agagataaag ccgatccttg
     4921 cgctgtttgt agacgttggt gttgtgcgcg cgatagcctt tgtcgagctt gtgccgcggc
     4981 aacggtgttt cgcccttttt ggtgcccggc atccaccagc gccccacttc gacgggtttt
     5041 gacggattgc gcacgtctat gcagcggtaa aactggtcgt cgagcgggtg gctcggctcg
     5101 aagtccggcg cgccggatgc catgtgcaca tattctccgt cgcagaacca gagttggtgc
     5161 acgccgcgcg aattggcgcc cgagcaatcg aggaaggaaa ttgaacgggg cttttcgggc
     5221 accgacaggt caaacagctc aagcccggcc ggctgctggt tcaccttctg ggtctggtac
     5281 gcaaccgcca tgatatcgcc ggacagctcc agcgagttgg agcgcatgta aggcttcggc
     5341 aggtcggtct gcaccacgac cttcggattg cgcgggtccg aaacgtcgac gccggtaaaa
     5401 tttttcggcg cgctttcgtg cgccagccag atgattcgcc ggccatcctt ggcaagctgc
     5461 accgacatgc cttcgcccat accgccgaag ccgccgaggt cgttctgtgc gagaagcttg
     5521 aaattccagg tgacaggctc accgcttttg ttcagcggat tgttggcggc ggacatcgtg
     5581 tactccgggg ttcgtcagat tcttgcagaa aggccggcgc gcatgcgccg gccctgggtt
     5641 gaacaatggc tgcttgctca ggccattgcc ttcttcagga aatcggtgcg gaaggccttg
     5701 ctccagtccg ggatggcctt gagcttgttg gccttcacca ggatttcgct gtgatggttc
     5761 atgtagggaa tgaggtcagt cggccagccg ggctgcacct cgacccgtgc aagggccttg
     5821 gagaaggtgt ccttcgacat ggtgtaaccc ttcgacgtgt agaacgagta aatcgtgtcg
     5881 gcgaccttgc cggggcttga tttgaaatct ttcgccactt cgagccaagc cttgagatag
     5941 gccactacag tgtcaggact tttgtcgacg aattccggcg tggcggccat gaacaccggg
     6001 agcttgtcga agtcgtagaa ctcggaaatc gtgacgccga gaccctcggc ttccgcgatt
     6061 gcgttgtagg gctcgacgtt gaccatcgcg tccacggtct tggcggccat ggccgccacc
     6121 atgtcgttca cgttcatgcg gatttcctgg tagtcgccct tctgcaaccc ggccttcggt
     6181 gcgatctggt cgacgaagat gttgccggtt gaggaaccgg tctggttggc gactttcttg
     6241 ccctttagct gctcgacctt ggtgatcccg aggtccttgc gcgcgagcac gcgggccgtc
     6301 ttgccgacat attcgatcat tgcgatggcg acgagcccgc ctttgtcatg gcctatggcg
     6361 aaggagggac cggcataagt gcccatgtcg acctgacgtg ccaccatctg ttgcatcgtg
     6421 agattgccgg acggcaccgc gaactcctcg aacttgaccc cgtgcttttc aagcaggccg
     6481 gatcgcagca aatagtaggt cggcatgccc cacatattgg tttggtagcc ttggcgtacg
     6541 gtttccagcg cgagtgcagg ccgcacgcca aatcccgctg ccagtacgcc ggcgccggtc
     6601 gccgccagcg catgacggcg ggttattcct ttgttcgaaa tggtcattga tgttcctccc
     6661 tatgatctgg ccttgccatt catggctttc gtttcgccgg catcatggcc gagatgccgt
     6721 agcaactcct tctggcattc gacaaattcg acgctcagcg gatcgcgcgg acgcggcaga
     6781 tcgattttca tttctgtctt caccgtgccg ggatgcggcg acatgactat cacgcggtcc
     6841 gcgagatata ccgcctccgc aacgttgtgc gtcacataga tcaccgtctt gcgcgtctca
     6901 cgccaaacat cggcaagcaa ccgttgcata tcatcacggg taagcgcgtc caaagccgca
     6961 aacggctcat ccatcaagag aaccgaagga ttgtacgcca atgcccgtgc aatggcgacc
     7021 cgctgctgca ttccgccgga aaggtggtga ggataggacc cggtgaactt ctccaatttc
     7081 accaggcgca attgctcgcg tgcgatccgg ttgcgctcct cctttggcac acccttcatt
     7141 tccaggccga acgcgacatt ccccagcgcg gtgcgccaag gaaaaagctg ggcgaagtcc
     7201 tgaaagacga cgccgcgatc catgccgctg cccgtcaccg gcttaccgcc gatgcgaatc
     7261 tcgccggcgg ccggcgaaag aaatccggca atgatgttga gcagcgtcga cttgcc
//
LOCUS       DASSRL010000398         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1287921, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000398 DASSRL010000000
VERSION     DASSRL010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287921"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1046)
                     /locus_tag="VFL49_08130"
     CDS             complement(69..1046)
                     /locus_tag="VFL49_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7911985.1"
                     /translation="MRSTKISTRRSTLRSFAALAALALGAALGGNAQAAGYPERPIEF
                     IVPWGPGGGADQLARLISKLMEPMLGQGIPVVNVPGGTGATGITKLLAAPADGYSMAI
                     YIADSHALLAGKSPRWTMNDITPVAVMIKGPSFIFVKQDSPFKTWDDFAKEAKANPGK
                     LKVATLGFGSVDDFSLTVLDRAGIKVVQVPFSKPSERYVSILGGHADALYEQAGDVAQ
                     FLNSKQMRPILLFGEKRLPAFWDVPASYELGYKVALPQFRSIVVRAGTPPDVVKKLSD
                     ALAKVYESPEYKKFLEDQFADPNSFEDASKAPAFVNEQLNDMKKTVATN"
     gene            complement(1329..>2414)
                     /locus_tag="VFL49_08135"
     CDS             complement(1329..>2414)
                     /locus_tag="VFL49_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HET7911986.1"
                     /translation="CRRFGIHPDTAYKWIGRWTAGEMDMADRSRRPHNSPARTTAAVE
                     TQVLKLRDEHPVWGARKIVRCLTRAGTVSPAFSTVHQILRRNDRIIPPPGGAAAQQRF
                     EKAAPNLLWQMDFKGWFKLGNGARCHPLTVLDDHSRYALCIAACADEQGRTVQSRLEP
                     VFRRYGLPDAFFVDNGGPWGDSSGHRWTRFGVWLLKLGIDVLHSRPYHPQSRGKNERF
                     HRTLKAEVLAFKHLSDLPAAQTAFDRWRAVYNLERPHEAIEQDVPASRYRVSTRAMPD
                     RLPPVDYDDHEIVRTVPASKDYIRFKGRLWKVPQAFRCERVAIRPLTADGCYGIFFGA
                     HQIASIDLTKPECVGDVSEQVSAMSSD"
BASE COUNT          415 a          743 c          789 g          467 t
ORIGIN      
        1 atcctgtaca gccagtggtc gaccgccctg gatccccgct ttcgcgggga tgagcggccc
       61 aaagccgctc agttcgtcgc caccgtcttc ttcatgtcgt tgagctgctc gttgacaaag
      121 gccggcgcct ttgaagcgtc ctcgaagctg ttcggatcgg caaactggtc ttcgagaaac
      181 ttcttgtact cgggcgactc gtagaccttg gccagcgcgt ccgagagctt cttcacgacg
      241 tcgggaggcg tgccggcacg caccacgatc gagcggaact gcggcagcgc gaccttgtag
      301 ccgagctcgt aggacgcagg cacatcccag aaggccggca gccgcttttc gccgaacagc
      361 aaaatcgggc gcatctgctt gctgttgagg aactgcgcga cgtctcctgc ctgctcgtag
      421 agcgcatcgg cgtggccgcc gagaatcgag acgtagcgct cgctcggctt ggagaacggc
      481 acctgcacca ccttgatgcc tgcgcgatcg agcacagtga gcgagaagtc gtcgacgctg
      541 ccgaaaccga gcgttgcgac cttcaacttg cccggattgg cctttgcctc tttcgcgaaa
      601 tcgtcccagg tcttgaacgg gctgtcctgc ttcacaaaaa tgaaggacgg acctttgatc
      661 atcaccgcga cgggcgtgat gtcgttcatc gtccagcgcg ggcttttgcc ggccagcagt
      721 gcatggctgt cggcgatgta gatcgccatc gaatagccgt cggcgggcgc ggcgaggagt
      781 ttcgttatgc cggtggcgcc ggtgccgccg ggcacgttca ccaccggaat gccctgaccg
      841 agcatcggct ccatgagctt gctgatgaga cgggccagct ggtcggcgcc gccgccgggg
      901 ccccacggca cgatgaattc aatcgggcgc tcgggatagc cggctgcctg cgcattgccg
      961 ccgagcgcgg cgcccagcgc caacgccgcc agcgctgcga agcttcgcaa ggtcgaacgc
     1021 ctggtcgaaa ttttggtcga acgcatggac ttgatcctcc ctgccatcgg ccgcctgttt
     1081 gttgcgtccc gcccgcggcc gtgcccggca gggacattgc tccggctttg ggctgccggt
     1141 ttacgcgacg aattctcatt atttgagact gctattctca atacgataac agatgagcat
     1201 cggcccgcaa cggtcaatcc gagccgtttt gtctcgcgat gcgggaattt gattttaagt
     1261 tccgcgctat gcgcagaagg ttttgaattg cgcgcgtctt acggatgcgt catggccggg
     1321 cttgttgttt agtccgagga catggccgac acctgttcgg agacatcacc gacacattcg
     1381 ggcttggtca agtcgattga agcgatttga tgggcgccga agaagatgcc gtagcagcca
     1441 tcggcggtga gcgggcggat ggcaacgcgc tcgcagcgga aggcctgcgg cactttccag
     1501 agacggccct tgaacctgat gtagtctttg ctggcgggaa cggtgcggac gatctcgtgg
     1561 tcgtcgtagt cgaccggcgg gaggcggtcg ggcatggcgc gggtgctgac ccgatagcgg
     1621 cttgccggga cgtcctgctc gatcgcttca tgcggccgct cgagattgta aacggcgcgc
     1681 cagcggtcga aggctgtctg cgccgcgggc aaatcgctca ggtgcttgaa cgccaggacc
     1741 tcggccttca gcgtgcggtg aaagcgctcg ttctttccgc ggctctgcgg gtgatagggc
     1801 cggctgtgca gcacatcgat gccgagcttg agcagccaga cgccgaagcg ggtccagcga
     1861 tgacccgatg aatcgcccca cggcccgcca ttgtcgacaa agaatgcgtc cggcagtcca
     1921 tagcggcgga agaccggttc caatcggctc tgcacggtgc ggccctgctc gtcggcgcag
     1981 gccgcgatgc aaagcgcata gcgcgaatgg tcatcgagca cggtcaaggg atgacagcgt
     2041 gcgccattgc caagcttgaa ccagcctttg aagtccatct gccacagcag gttcggggcg
     2101 gccttctcga agcgctgctg cgctgcagct ccccctggcg gcggaatgat ccggtcgttg
     2161 cgccgtagaa tctggtgcac cgtggagaaa gccggcgaga ctgttcccgc gcgcgtcagg
     2221 cagcggacga tcttgcgggc gccccaaaca ggatgctcgt cgcgcagctt gagcacctgc
     2281 gtctcaacgg ccgcggtcgt gcgcgcgggg ctgttgtgcg gacggcgcga gcggtccgcc
     2341 atgtccatct cgccggccgt ccagcggcct atccacttgt aggccgtgtc aggatgaatt
     2401 ccgaagcgcc ggca
//
LOCUS       DASSRL010000399         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_745654, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000399 DASSRL010000000
VERSION     DASSRL010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745654"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFL49_08140"
     CDS             complement(<1..1005)
                     /locus_tag="VFL49_08140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7911987.1"
                     /translation="MAAALAAKVLPFGLFETRRLLGAAVGIVGLIATWRVGRRLAGPI
                     AGLIALLLLAACPLYYGHMFMNAKDAPFATAMAVLLLAVVRAIDEYPRPGVPTIALLA
                     TGLGLAFGSRILAGIAAPSAVIGLLVILVVEVRALGAKKALAKLGTFVVRSIPALVIG
                     YLVMGLLWPWSVLAPLNPIRAAEYFDTFFEKPWREMYAGHLFAVTEMPVSYLPHLFVL
                     KMPEIMLALGLAGTVGALIAVGSRQLPPTRRANLLLVSAWAFLPVAVAMLTHPALYNG
                     LRHFVFVLPPFAVLGGIAGAWMLYRAKAFGGWAAAALVALFAGGLALPISDMVRLHPY
                     Q"
     gene            complement(1274..1876)
                     /locus_tag="VFL49_08145"
     CDS             complement(1274..1876)
                     /locus_tag="VFL49_08145"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7911988.1"
                     /translation="MPFTLPDLPYAHDALAPYMSRETLEYHHDKHHKAYVDNGNKLLE
                     GSGLENKSLEEVVKASFGKNAGLFNNAGQHYNHTHFWKWMKPNGGGDKIPGKLKAKID
                     SDLGGVAKMKEDFTQAGVTQFGSGWAWLAVKDGKLIVSKTPNGENPLVHGATPILGCD
                     VWEHSYYIDYRNRRPDYLKAFLDHLVNWEHVEEMLVAATK"
     gene            2163..>2737
                     /locus_tag="VFL49_08150"
     CDS             2163..>2737
                     /locus_tag="VFL49_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911989.1"
                     /translation="MAVLLFSVGTLALVAGLVMVGFGIPINEFSFGNTLIGAGTTAAV
                     GGLIIIALGVAVSQLRRIAESLAGDLSARLAQPMDIMEATDASMPGVSAPGRVPFPAR
                     PKSETPMAGLGRSPGVPGSFARDGGSDQFAPTLRNPEEAPMAVEDEISLSPPQPAPDS
                     FADRAKPLGPPTVGGGEKGLSDTSQEPAAGW"
BASE COUNT          589 a          772 c          850 g          526 t
ORIGIN      
        1 ctgatagggg tgcagccgca ccatatcgct gatcggcaac gccagtccgc cagcaaacaa
       61 agcaacgagt gcggccgccg cccatccgcc aaaagccttt gcccgatata gcatccatgc
      121 accggcgatg ccgccaagca ccgcgaaggg cgggagaacg aagacgaagt gccgcaagcc
      181 gttgtaaagc gcgggatgtg tcagcatcgc aacggcgacc ggcaggaaag cccaggctga
      241 gactaaaaga agatttgcac ggcgggtcgg cggcagttgg cgcgaaccga cggcaataag
      301 agcgccaacg gttccggcaa gcccaagtgc aagcataatt tccggcatct tgagcacaaa
      361 aagatggggc aggtaactca ccggcatttc agtgacggca aataaatgtc cggcatacat
      421 ctcacgccag ggcttttcga aaaacgtgtc gaagtattcc gcggcgcgga tcggattgag
      481 aggcgcaagc acggaccatg gccacaacag acccatgacc aagtaaccga tcacgagggc
      541 cggaattgat cgcacgacga acgtgccgag ttttgcgaga gcctttttag cgccaagcgc
      601 acggacctcg acgacaagga tgacgagaag acctatcacg gcggacggcg ctgcaatccc
      661 ggcaaggatg cgggaaccga atgcaagccc gaggcccgtt gcgaggagcg caatcgtcgg
      721 cacgcccggc cgcggatatt catcgattgc tcggacgacc gcgaggagca gtacggccat
      781 ggctgttgcg aagggagcat ccttcgcatt catgaacata tgaccgtagt aaaggggaca
      841 cgccgcaagg agcagcagcg caattagtcc ggcgattggc cccgcgagcc ggcggccaac
      901 gcgccaggtt gcgatcagac cgactattcc gacggcggcc ccgaggaggc gccgtgtctc
      961 gaatagcccg aacggcagca ctttcgctgc aagcgcggcg gccatgtcga agccgccgcc
     1021 gtatttgtaa agattgacga atgaaagcgc ccgctgatct gaaaagccgg aaccgtataa
     1081 cgtgagcagc agatcgccgt attcggaatg ggtgtagtcg tcccatccga ggccgtagtc
     1141 gcgaaacgtc gccgcggcga tgactgcaat ggcgacgagg gcaacaagtg ccgctcgatc
     1201 ggagaatatg tccgccgacc ggcaccagga tgtttccgaa gaatccggcg aggtttggtc
     1261 ccctgcgctt gccctacttg gtcgccgcga cgagcatttc ttcgacgtgc tcccagttca
     1321 ccaagtggtc caggaaagcc ttgagatagt cgggtcgccg attgcggtaa tcgatgtagt
     1381 aggaatgctc ccacacgtcg cagccaagaa ttggcgttgc accgtgaacg agcgggtttt
     1441 cgccattcgg cgtcttgctg acgatcagct tcccgtcctt gaccgcgagc caggcccagc
     1501 ctgagccaaa ctgcgtcacg ccggcctgcg tgaaatcttc cttcatcttg gcgactccgc
     1561 ctaggtcgct gtcgatcttt gccttgagct tgcctggaat tttgtcgccg ccgccgttcg
     1621 gcttcatcca cttccagaaa tgcgtgtggt tgtaatgttg ccccgcattg ttgaagagac
     1681 cggcgttctt gccgaacgac gctttcacga cttcctcgag tgatttgttt tcgagcccgc
     1741 tgccttccaa aagcttgttg ccgttgtcga cgtaggcttt gtgatgcttg tcgtgatggt
     1801 attcgagtgt ttcgcgcgac atatagggcg cgagcgcgtc gtgagcatat ggcaggtcgg
     1861 gcaacgtgaa cggcatcaga tcctcctcgg tgcacaaacg atgactcgga ataaactggt
     1921 tctaggatga aaccggcctg tcccggacga gttcctcaat gaagctaatt aggcggtagc
     1981 gcggctctcg gcaacgtttg aaaacgaact ctgtgagcat tacccatcag gatgaggcga
     2041 agcagacacc gtcgatttag tggttgccgc cggctcgtga aaaagcgaga atttgccgtt
     2101 gtcagaagaa tttagccggt aagtcgcggc gaaagttgct ggtcgggagg ggatcgtaaa
     2161 gtatggcagt attgctgttc agcgttggca ctttagcctt ggtggctggg ctcgtgatgg
     2221 tcggattcgg gattccgatc aacgagttca gcttcggaaa tacgcttatt ggcgccggca
     2281 ccacggcagc ggtcggcggc cttattatca ttgcgctcgg tgtcgccgtc agccagttgc
     2341 ggcggattgc cgagtcgctg gcaggcgacc tgtcggcgag gcttgcacaa ccaatggata
     2401 tcatggaggc caccgatgct tccatgcctg gagtcagtgc gccgggccgt gttcctttcc
     2461 cagcgcgccc gaaatcggag accccgatgg cgggattggg gcgttcgcca ggtgtaccgg
     2521 ggagttttgc gagggacggt ggcagcgacc aatttgcgcc gaccttgcgc aacccggaag
     2581 aagcgccgat ggcggtggag gatgaaattt ccctgtcgcc tcctcaacct gcccccgatt
     2641 cctttgccga ccgcgcaaag ccgcttggac cgccgactgt gggcggcggt gagaaaggcc
     2701 tttcggacac gagtcaagaa ccggccgcag gctggag
//
LOCUS       DASSRL010000400         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_132993, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000400 DASSRL010000000
VERSION     DASSRL010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132993"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFL49_08155"
     CDS             complement(<1..367)
                     /locus_tag="VFL49_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7911990.1"
                     /translation="MGEFAVGQGVSRFEDPRLVRGGGRYTDDIKLPGLAHGVVLRSPH
                     AHAKMRSIDTSSAKKAPGVLAVLTSADIKAAGYGDLPVPGGLKRRDGSPMYKPRYPIL
                     AEDRVRWVGDCVAFVVAETV"
     gene            complement(436..1503)
                     /gene="hemB"
                     /locus_tag="VFL49_08160"
     CDS             complement(436..1503)
                     /gene="hemB"
                     /locus_tag="VFL49_08160"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7911991.1"
                     /translation="MAIKFGRPIESRTRFTPVETNSTARDEQLDLSVRMRRNRRSEWA
                     RRMVRENILTTDDLIWPLFVMDGTNARAPVPSMPGVERLSVDEVAREVARAAKLSIPC
                     IALFPYTDPKLRDEAGSEALNPDNLVCRAIRAIKKEVPEIGVLCDVALDPFTSHGHDG
                     LLRDGVILNDETVAVLVKQALVQAEAGCDIIAPSDMMDGRVGAIRKALDAAKFADVSI
                     MSYAAKYASAFYGPFRDAVGSAKTLTGDKRTYQMDFGNSDEALREVALDIEEGADMIM
                     VKPGLPYLDIVRRVKEIFAMPTFAYQVSGEYAMIMAAAQNGWLDGERAMIESLTAFKR
                     AGCDGVLTYFAPRVAEKLNAG"
     gene            complement(1596..3122)
                     /locus_tag="VFL49_08165"
     CDS             complement(1596..3122)
                     /locus_tag="VFL49_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7911992.1"
                     /translation="MASDSKASPGPDLSRGVPASDLMDGAMMTGHIGDDEVLLVRQNG
                     KLFALSAHCTHYHGPLADGLLVGATVRCPWHHAHFSLETGEAIAAPALSPLSCWQVEE
                     RDGKIFVKGKRAQAAAKKTGSASEEVVIVGGGGAGFAAAEMLRRRGFAGRITMLSNDQ
                     AAPVDRPNLSKDYLAGSAPEDWVPLRSNDWYTENQIDLKLKADVTSLDAKNKELSVAG
                     GSKIKFDKLLLATGAEPVKLDIPGADQSHVHTLRSLADCRAIIEQAKTAKRAVVIGAS
                     FIGLETAAALRTRDIEVDVVAPEKCPLERVFGPQLGDFIRTLHEEHGVTFHLENTVTA
                     IERSRATLRSGETLDADLVVIGVGVRPRIALAEKAGLAIDKGVSVNEHMETSATGIYA
                     AGDIARWPDPISGERIRVEHWVVAERQGQVAALNMMGIPTLFRSAPFFWSQHYDVPIN
                     YVGHAEKWDTIDIEGEVMKRDCVVRYKRAGRVLAVASIYRDKASLEAGVTMERAVARA
                     "
BASE COUNT          569 a          993 c          998 g          671 t
ORIGIN      
        1 caaccgtctc ggcgacgaca aaagcgacgc aatcgccgac ccagcgcacc cggtcctcgg
       61 ccaggatcgg atagcgaggt ttgtacatag gcgagccatc acggcgcttg aggccaccag
      121 gcaccggcaa gtcgccgtaa ccagctgcct tgatgtcggc actggtcagc accgccaaca
      181 cgccgggcgc ttttttggcc gaggaagtgt caattgatct catttttgcg tgcgcgtgtg
      241 gcgagcgaag aacgacgcca tgagccagtc cgggcaactt gatgtcgtcc gtataacgcc
      301 cgccgccgcg gaccaatcgc ggatcctcaa agcgagacac gccttggccg acggcgaatt
      361 cacccatgga acgtcaacct gtcgaattca taggtcactg ggcgggtgac cgggagggaa
      421 agtgcccttg tcgcgtcaac ctgcgttgag tttttccgcc acgcgcgggg cgaaataggt
      481 cagaacgccg tcgcagccgg ctcgcttgaa cgctgtcagg ctttcgatca tcgcgcgctc
      541 gccgtcgagc cagccgttct gcgccgccgc catgatcatc gcatactcgc cggacacctg
      601 gtaagcgaag gtcggcatcg caaagatttc cttcacgcgc cgcacgatgt cgagataggg
      661 caggccgggc ttgaccatga tcatgtcggc gccttcctcg atgtcgagcg caacctcgcg
      721 cagcgcttcg tcggagttgc cgaaatccat ctgataagtg cgcttgtcgc ccgtgagtgt
      781 tttcgcggag ccgaccgcgt cgcggaaagg cccataaaac gccgacgcat attttgcggc
      841 gtaggacatg atcgagacgt cggcgaattt ggcggcatcg agcgccttcc ggatggcgcc
      901 gacgcgtcca tccatcatgt cggaaggtgc gatgatgtcg cagccggctt cagcttgcac
      961 aagggcctgc ttcacaagca cggcgactgt ttcgtcgttg agaatgacgc cgtcgcgtag
     1021 caggccgtcg tggccatggc ttgtgaaggg atcgagcgct acgtcgcaca ggactccgat
     1081 ctccggcacc tcctttttaa ttgcgcgaat ggcgcggcag acaaggttgt ccggattgag
     1141 cgcctcgctt ccggcctcgt cgcgcaattt cgggtcggtg taggggaaaa gtgcgatgca
     1201 ggggatgctc agcttggcgg cgcgtgcgac ctcccgcgcg acctcatcga ccgacagccg
     1261 ctcaacgccc ggcatcgacg gaacgggagc acgcgcgttt gtgccgtcca tcacgaacag
     1321 cggccagatc aggtcgtcgg tcgtgagaat attctcgcgc accatgcggc gcgcccattc
     1381 gcttcgccgg tttcggcgca tgcgcacgga gagatcaagc tgctcgtccc tggccgtcga
     1441 gtttgtttcg accggggtga acctggtgcg gctttcgata gggcggccga atttgatcgc
     1501 cataagggtt actcccggcg atctatttag ccggtctcgg caggtcacgc tacctgcgct
     1561 gcgcatggac gttaagcgtg tctatcgggc ggattctacg ctcttgcgac cgcgcgctcc
     1621 attgttacgc cggcctcaag gctcgccttg tcgcggtaaa tcgaggcaac tgccagaacg
     1681 cgtccggcgc gcttgtaccg gacgacacag tcgcgcttca tgacttcgcc ttcaatgtcg
     1741 atggtatccc acttttcagc gtggccgaca taattgatcg gcacgtcata gtgctggctc
     1801 cagaagaagg gcgccgagcg gaagagggtc gggataccca tcatgttaag ggccgcgacc
     1861 tgaccttgcc gttcggcgac gacccaatgt tcgacgcgga tgcgttctcc agaaatagga
     1921 tcgggccagc gcgctatgtc gccggccgcg tagatgccgg tggcacttgt ttccatgtgc
     1981 tcgttcacgc tcacgccctt gtcgatcgcg agtccggctt tttctgcaag ggcgatccgc
     2041 gggcgcacgc cgacaccgat cacgacgagg tcagcgtcga gcgtttcccc gctcctgaga
     2101 gttgcccggc tgcgctcaat cgccgtgacg gtattctcga ggtggaatgt gacgccgtgc
     2161 tcttcgtgca gcgtacgaat gaagtcacca agctgcggcc cgaacacccg ttcaagcggg
     2221 catttctcag gtgcgacgac atcgacttcg atatcgcgtg ttcgtaacgc cgcggctgtc
     2281 tccaggccga taaagctcgc accgatgacg acggcgcgct tggccgtctt tgcctgctcg
     2341 atgatcgcac gacaatctgc gagagatcgc aacgtgtgca cgtgcgattg atctgcaccg
     2401 gggatgtcga gcttgaccgg ctcggcaccg gttgcgagca gcagtttatc gaacttgatc
     2461 ttgctgccgc ctgcaactga aagttctttg ttctttgcat cgagcgacgt cacgtcggct
     2521 ttgagtttaa ggtcaatctg gttttcggtg taccagtcgt tgctacgcaa cggcacccag
     2581 tcttcgggag cgctgccggc caggtagtct ttcgacaaat ttggccggtc gaccggcgca
     2641 gcctggtcgt tgctgagcat ggtaatccgt ccggcgaagc cgcggcggcg caacatctcg
     2701 gctgcggcga atcccgcgcc gccgccgccg acaatcacga cctcttcgct tgccgatccg
     2761 gttttcttcg cggctgcctg agctcttttg cctttgacga aaattttgcc gtcacgttcc
     2821 tcgacctgcc agcagctgag cgggctgagc gcgggcgccg cgatcgcttc gccggtctcg
     2881 aggctgaaat gcgcgtgatg ccatggacag cgcaccgtgg cgccgaccag gaggccgtcg
     2941 gcaagcggcc cgtgatagtg cgtgcagtgt gcggacagcg caaacagctt gccgttctga
     3001 cggacgagca gcacttcgtc gtcgccgata tggccggtca tcatggcgcc gtccatgaga
     3061 tcgcttgccg ggacacctct tgagagatcg ggcccgggtg atgctttgct gtcgcttgcc
     3121 atcgtttcct ccgcactgcc tgcttatcag ggttggatgc tccagccgcc ggaaaattgc
     3181 agcataaggc attaatgctt gtcgggcctt cccggttgcc gcggtaaggg c
//
LOCUS       DASSRL010000401         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_588115, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000401 DASSRL010000000
VERSION     DASSRL010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588115"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VFL49_08170"
     CDS             <1..151
                     /locus_tag="VFL49_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET7911993.1"
                     /translation="GGHVIAQDEESSVVWGMPGQVTNAGLCSAVLPLPEIGGKLTRLF
                     TGERL"
     gene            148..1020
                     /locus_tag="VFL49_08175"
     CDS             148..1020
                     /locus_tag="VFL49_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET7911994.1"
                     /translation="MTPQGFDYLRKLLRERSGLVLAAEKQYLAESRLLPVARRNGMNT
                     IDELMDRLKSKAPVSLTTQVVEAMTTNETFFFRDKLPFDHFRDTIMPALIAARVREKR
                     IRIWCAAASTGQEPYSLAMLLKGMGSAISGYKIDIVATDLSSEILQKAKAGIYTQFEV
                     QRGLAAQLLVKYFDQNGESWQLAPELRGMVQFRPLNLLNDFSALGTFDLVFCRNVLIY
                     FDQDTKAAVLDRIARQMPDDGYLVLGAAETVIGLTDAFRPLADRRGLYVPTSGEKQAV
                     RGADNVIRLAALKA"
     gene            1130..1573
                     /locus_tag="VFL49_08180"
     CDS             1130..1573
                     /locus_tag="VFL49_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7911995.1"
                     /translation="MGQPLTIVGGAARGGWLILIDYVDPVLVVDDLNTMTRIVDGMLR
                     KCGFTNVEQCHDGPTALQKLREKPFLFMMTDREMPGMNGLELVREIRADPKIRNVPIV
                     IMSASSEAQHAEAAIAAGANFFMTKPFNLTTLKDVITQLMRPPQG"
     gene            1665..>2714
                     /locus_tag="VFL49_08185"
     CDS             1665..>2714
                     /locus_tag="VFL49_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7911996.1"
                     /translation="MQRQDWMMRPKQRMRAFRGRAQAIGSRFIFPLVVVAPLAGLAAA
                     AVIEAPKPQPAQKYLGAKVSGPNYTVKPTVRSDGVMRIFDVDTTYGKFTFDGVEFAKL
                     RLHELEATAAIEKMSQSEQFGQAFGRAALGPIKFGADLITKPADTVERSLSGIGNMLD
                     RVGAGLSNTRADRDNVMESLLGVSDTQRQLAVELDVDPYTDFPPLAERLKQMAGAMAG
                     GGLPVRAGLALVPGGVGIAVSSVASVDTAKDSLRNKTAAQVIAEARSILLSLGIPPEN
                     IDRLVENRNYTPADLLIMAQALKRLGAANTVIFVDKAAGAGSRNVAFYHRRRAQILAS
                     RNNDLGGLVSFVSVGG"
BASE COUNT          525 a          809 c          848 g          532 t
ORIGIN      
        1 cggcgggcac gtgatcgcac aggatgaaga gagcagcgtg gtctggggca tgccgggcca
       61 ggtaaccaat gccggcctgt gttcggccgt gcttccgttg ccggagatcg gcggaaagct
      121 cactcgcctc tttaccgggg agcgtctgtg accccgcagg gttttgacta tctgcgcaag
      181 ctgctgcgcg agcgctccgg cctggttctg gccgcggaga aacaatatct cgccgagagt
      241 cggctgctgc cggttgcacg ccgcaacggc atgaacacga tcgatgagtt gatggatcgg
      301 cttaaaagca aggcaccggt ttcgctcacc acgcaagtgg tggaagcgat gaccacgaac
      361 gagactttct tcttccgcga caagctgcca ttcgaccatt ttcgcgacac gatcatgcct
      421 gcgctgattg cggcacgtgt gcgtgagaag cggatccgca tctggtgtgc cgcggcttcc
      481 accggccagg aaccatattc gcttgcgatg ctgctcaaag gaatgggctc ggcaatttcc
      541 ggctacaaga tcgatattgt cgccaccgat ctgtcatcgg agattttgca gaaggcgaaa
      601 gccggaatct acacgcagtt cgaagttcag cgcgggctcg cggctcagtt gctggtaaaa
      661 tacttcgacc agaacggcga aagctggcag cttgcaccgg aattgcgcgg catggtgcaa
      721 ttccgtccgc tcaacctgct caacgacttc tcggcgctgg gcacattcga cctggtattc
      781 tgccgaaacg ttctgatcta cttcgatcag gacaccaagg ccgccgtgct cgatcgaatc
      841 gctcggcaga tgcccgatga cggttacctt gtgctgggcg cagccgagac cgtcatcggc
      901 cttacagatg cgttcaggcc actcgcggac agacgtgggc tctatgtccc gacgagcggc
      961 gagaaacaag ccgtgcgtgg cgcggacaat gtcatccgcc ttgcggcgtt aaaagcttaa
     1021 gacatccagt caagattaat attgcccgtc gatccgcgtc ggcggcttga tctctgtacg
     1081 aatcgtgtag attagaggtg tcgggctggc gcgtgttttg tcggcgcgcg tggggcagcc
     1141 cctcacgatc gtgggcggtg ctgcccgagg gggatggctg atcttgatcg actatgtcga
     1201 tccggtgctg gtcgttgacg acctgaacac gatgacgcgc atcgtggatg gcatgctgcg
     1261 caaatgcggc ttcaccaacg tcgagcaatg ccacgacgga cctaccgcgc tgcaaaagct
     1321 gcgtgaaaag cctttcctgt tcatgatgac cgaccgtgaa atgcccggta tgaacgggct
     1381 cgagcttgtg cgcgagattc gtgccgaccc gaaaatccgc aacgttccca tcgtcatcat
     1441 gtccgccagt tccgaggcgc agcatgcgga ggcggccatc gccgccggcg caaatttctt
     1501 catgaccaag ccgttcaatt tgacgacgtt gaaggacgtg attacgcagc tgatgcggcc
     1561 gccccagggc taagtggttc ttccttgcgc atgggtcgct ttccccgcgg ttgcgccgga
     1621 tgctatgttg ggcattgaaa aaacggccgt tccccggggg acaaatgcaa cgacaggact
     1681 ggatgatgcg cccgaagcag cgcatgcgtg cgttccgcgg gcgagcgcaa gcaatcggaa
     1741 gcaggttcat tttcccgctg gtcgtcgtcg ctcctctcgc cggattggcg gcggcagccg
     1801 tgatcgaagc gccaaaacct cagcccgcgc agaagtatct cggcgcgaag gtgagcgggc
     1861 ctaactatac ggtcaagcct acggtgcgca gcgacggtgt catgcgcatc ttcgacgtcg
     1921 acacgacgta tgggaaattc acctttgacg gcgtcgagtt tgcgaagctg cggctgcacg
     1981 aacttgaggc caccgcggca atcgagaaaa tgtcgcagtc ggaacagttc ggccaggcct
     2041 ttggccgcgc ggcgctcggc ccgataaaat tcggagccga cctcatcacc aagcccgccg
     2101 acaccgtcga gcgttcgctg agcggcattg gcaacatgct cgaccgcgtc ggcgcggggc
     2161 tgtcgaacac gcgcgcggac cgtgacaacg tgatggaaag cctgcttggt gtcagtgata
     2221 cgcagcgtca gctcgcggtc gaactcgacg tcgatcctta caccgatttt ccgccgctgg
     2281 cggaaaggct caagcagatg gccggcgcca tggcgggcgg cggtctgccc gtccgcgccg
     2341 ggctggcgct tgtccctggt ggggtcggaa tcgccgtttc gtcggtcgcg agcgtggaca
     2401 ccgcaaagga cagtttgcgc aacaagaccg ctgcgcaagt cattgccgaa gctaggtcga
     2461 tcctgttgtc gctcggaatt ccgcccgaaa acattgaccg cctggtcgag aaccggaact
     2521 acacgcccgc ggatctgctc atcatggcgc aggcgctcaa gcggctcggc gccgcgaata
     2581 ccgtgatatt cgtagacaaa gccgcgggtg ccggctcgcg caacgtggca ttctatcacc
     2641 gccgccgtgc gcagatcctc gcgtcgcgca acaacgatct cggcggcctt gtatcgttcg
     2701 tctcggtcgg cggc
//
LOCUS       DASSRL010000402         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1305460, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000402 DASSRL010000000
VERSION     DASSRL010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305460"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..272)
                     /gene="rpmA"
                     /locus_tag="VFL49_08190"
     CDS             complement(6..272)
                     /gene="rpmA"
                     /locus_tag="VFL49_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7911997.1"
                     /translation="MAHKKAGGSSRNGRDSAGRRLGVKRYGGEQVLAGNIIVRQRGTK
                     CHAGANVGMGKDHTLFALVPGKVEFRTKAKDRVLVSVLPMEAAE"
     gene            complement(341..745)
                     /gene="rplU"
                     /locus_tag="VFL49_08195"
     CDS             complement(341..745)
                     /gene="rplU"
                     /locus_tag="VFL49_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7911998.1"
                     /translation="MFAVIKQGGKHYTVAADQTIELGKIAAKPGEIVTFPVTVLGGDK
                     PSVGVPLVSGALVAAEVLAHTRGDKVIAFKKRRRQNSRRKRGHRQDYTMVRITEILTD
                     GKQPSKQATGKRAPGKPKAAKKPKAEKKAAEA"
     gene            1215..1304
                     /locus_tag="VFL49_08200"
     tRNA            1215..1304
                     /locus_tag="VFL49_08200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1249..1251,aa:Ser,seq:cga)
     gene            1435..2361
                     /locus_tag="VFL49_08205"
     CDS             1435..2361
                     /locus_tag="VFL49_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7911999.1"
                     /translation="MRQLKYFIGVVEAGNMTRAAEQLHVAQTALGMQIRQLEEDLGVA
                     LLMRHSRGVEPTKAGSLLHARALAILNDVEQARQDVRACERQDSEAIRLGITPALMLV
                     CGTEIAMTVREQLPNVFLSIVEAMSHVLIETLARGEADFILCYDVPDLPQFSRIALLQ
                     DDLVLVTPPRAGNGKPIPFVEALEESIAMPEEGDSVRAVVARTARDLGLELKLAYEVR
                     SISAMKSLVMRGAASAILPYFAALDEVRAGKLDARPITMPAIKRTLFLASSRQRGPFK
                     NEAGLTGAVRSALTGLLDALGPLAQPLWVRTA"
BASE COUNT          476 a          818 c          811 g          556 t
ORIGIN      
        1 gccgtttact ctgcggcctc catcgggagt accgatacga gaacgcggtc tttggctttc
       61 gtgcgaaact caacttttcc tggaacgagt gcgaacagcg tgtggtcctt gcccatgccg
      121 acattggcgc ctgcgtgaca cttggtgccg cgttggcgca caataatgtt gccggcgaga
      181 acctgttccc cgccatagcg cttcacgccg agccggcgac ccgctgagtc gcgcccgtta
      241 cgtgaggaac cgcctgcttt cttgtgtgcc atcgttctga atcctgaatc tctcttacat
      301 cactttcatg accggcgcat tacctaattg cggtatgcgc ttatgcctcg gcggccttct
      361 tttcggcttt gggctttttc gccgccttcg gcttgccagg cgcccgcttg ccggtcgcct
      421 gcttcgaggg ctgcttgccg tcggtgagga tctcggtgat ccgcaccatg gtgtagtcct
      481 ggcgatggcc gcgcttgcgg cgcgaattct gccggcggcg cttcttgaag gcgatgacct
      541 tgtcgccgcg cgtgtgcgcg agcacttcgg ccgcgacgag cgcgcccgag acgagcggaa
      601 cgccgaccga cggcttgtcg ccgccgagga cggtgacggg gaaggtgacg atttcgccgg
      661 gcttggctgc gatcttgccc agttcgatcg tttggtcggc ggcgaccgtg taatgcttgc
      721 cgccctgctt gatgaccgcg aacatctctc aaatccggtt gttagggagg ggcggcggag
      781 cctgcccctc aataaaagcg cgcggggctc gccccgcgct gtttcgcggg gtttatgacg
      841 gcgcaggcgc tctctgtcaa gaaaaacggg gcaactataa agacagggcg ttgaaaaata
      901 taagaaaaat ccgagcgaat ctgcagaatt gcgttcgagg gcactgaaat tgcctcgaac
      961 acggcctcgt cctgaggaga ccgcgctagc ggccgtctcg aaggacgggc ctcatgcttc
     1021 gagacgccag ccacaaaagt ccgctgttgc ggacttttgg ctaaaactgc cgatgtcgca
     1081 tgaatgcgac atcggtggct ggctcctcag catgagggtt taaggggctc cggttgcatg
     1141 gcttgtctcg ccaaaaccgc acccctataa gttttcgcaa caaccctcag gaaaattatt
     1201 ccttcaagcc tcgcggagag gtggctgagc ggttgaaagc accgcactcg aaatgcggca
     1261 tactcgcaag ggtatcgggg gttcgaatcc ctccctctcc gccaatccgc tttgacaaag
     1321 tttctccggc gcgttcgtcc gctttttcgg agcttcagta cggatcgata aggtcgtata
     1381 tcaggcaagt tttcacccgg cagtccgccg atcgcttcaa tccctactat gaacttgcgt
     1441 cagctcaaat atttcatcgg cgtggtcgag gctggcaata tgacgcgcgc tgcggagcag
     1501 ctgcacgtcg cgcagaccgc gcttggcatg cagatccggc agctcgaaga ggacctgggc
     1561 gtcgccctgc ttatgcgcca ttcccgtggc gtggaaccga cgaaagccgg cagtctcctc
     1621 cacgcgcggg ccctcgccat cctgaacgat gtcgaacagg cccggcagga cgtgcgggct
     1681 tgcgagcggc aagacagcga ggcaatccgc ctcgggatta cacccgccct gatgctggtg
     1741 tgcggcacag agatcgccat gacggtgcgc gagcaactcc ccaatgtgtt cctcagcatc
     1801 gtggaggcga tgagtcatgt gctgatcgag acgctggcgc gcggcgaagc cgacttcatc
     1861 ctctgttacg acgttcccga tctgccgcag ttctcgcgca ttgcgctgct gcaagacgat
     1921 ctggtgctgg tcacgccccc gcgcgccggg aatggaaaac cgattccgtt cgttgaggcg
     1981 ctggaagaaa gcatcgccat gcccgaggag ggcgattctg ttcgcgcggt cgtcgcccgt
     2041 accgcgcggg acctcggcct cgaattgaag ttggcctacg aggtgcggtc gatttcggca
     2101 atgaaaagcc tcgtgatgcg aggcgccgcc tcggccatct tgccatactt tgcggcgctc
     2161 gacgaagtgc gcgccggaaa gctcgacgcg cgacccatca ccatgccggc gatcaagcga
     2221 accctctttc tcgcgtcgtc gaggcagcgc ggtccattca agaacgaggc ggggctcact
     2281 ggagccgtcc ggtcagcgct gaccggcctg ctcgacgcgc tcggtccact cgcgcaaccg
     2341 ctctgggttc gaaccgccta gctccacagc gtgcccgctg aaattctgcg cgcagccttc
     2401 actcgatcat attggacctg tggccggcgc atcgctcata tcgctcgcat cctctcgctc
     2461 gacaggggag atggctttgt caaaaggagg aatgccatga ggactgtgta tttcatcggg
     2521 ctcgtcgcgg cagcacttgc cttgtcgttt gcccgtgtga gtctcgcctc cgcggaaggc
     2581 tgtaatatcc aacaggcgac gctgatggag cctggatcga agaccggcga ggtcagcacc
     2641 gagcaactgc ggcgcattct c
//
LOCUS       DASSRL010000403         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1515655, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000403 DASSRL010000000
VERSION     DASSRL010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515655"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VFL49_08210"
     CDS             complement(<1..1108)
                     /locus_tag="VFL49_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET7912000.1"
                     /translation="MDAKTDDKSAGNCPFTHGHSNRDWWPNMLDLSVLHRNSNLSDPM
                     GEAFDYAKEFKRLDLKAVIKDLRALMTDSQPWWPADFGHYGGLMIRMSWHAAGTYRVH
                     DGRGGAGAGQQRFAPLNSWPDNANLDKARRLLWPIKQKYGRKISWADLMILAGNVALE
                     SMGFKTFGFAGGRSDVWEPEELYWGPEGTWLGDERYSGERQLAEPLGAVQMGLIYVNP
                     EGPNGNPDPVAAAKDIRETFFRMAMNDEETVALIAGGHTFGKTHGAGDPSLIGPEPES
                     GAIEDQGLGWKSKHGTGVGADAITGGPEVTWSNTPTKWSNHFFKNLFENEWELTKSPA
                     GAKQWKAKGAGASIPDAYDKSKKHVPTMLTTDLSL"
     gene            1230..2150
                     /locus_tag="VFL49_08215"
     CDS             1230..2150
                     /locus_tag="VFL49_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7912001.1"
                     /translation="MITLRQLRYLTSLARHRHFGRAAEDCAISQPALSMQVRELEREI
                     GAELVERRPNDIALTDVGLEVVERAERILAATRDLVDFARHRDLLSGQLKLGIIPTLA
                     PYVLPRVLPRLQSKYPYLRLEVRETQTKALLDELVRGELDCIMLALPAESTDVETMKL
                     FDDPFLLAVPSADEHPPRGRVSVDDVDQRRLILLEEGHCLRDQALAFCATTRRDVPAG
                     LGATSLATVMQMVANGYGVTLVPEVAAAVEVRDDRVKILRFAAPEPARTIGLAWRRTS
                     PRKTDFTAFSAVVTQTLTTNAWRAPARKAK"
     gene            complement(2198..>2734)
                     /locus_tag="VFL49_08220"
     CDS             complement(2198..>2734)
                     /locus_tag="VFL49_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET7912002.1"
                     /translation="KALTAARAELGEHAPRIEMFKIPTDKIREVIGTGGKVIREIVEK
                     TGAKINIEDDGSVKVASANGESIKAAINWIKSIASDPEVGHIYEGTVVKVMDFGAFVN
                     FFGAKDGLVHISQLAPRRVNKVTDVVKEGDKVKVKLLGMDERGKVRLSMKAVDQATGE
                     DIEGKGEQPQAAQGGAAE"
BASE COUNT          435 a          848 c          846 g          605 t
ORIGIN      
        1 gcagcgacag gtccgtggtc agcatcgtcg gtacatgctt ctttgacttg tcgtatgcgt
       61 ccggaatcga ggctcccgca cctttcgctt tccactgttt cgcgcccgcc gggctcttcg
      121 tcagttccca ttcgttttcg aacaggtttt tgaagaagtg gttgctccac ttcgtcggcg
      181 tgttgctcca ggtgacttcc gggccgccgg tgatggcgtc ggcgccgaca ccggtgccgt
      241 gcttgctctt ccagccgagg ccctgatcct cgatggcgcc actttccggc tccgggccga
      301 taagcgacgg atcgccggcg ccgtgggtct tgccgaaggt gtggccgccg gcaatcagcg
      361 cgaccgtctc ctcgtcgttc attgccatgc ggaaaaacgt ttcgcggata tccttggccg
      421 ctgcaacagg gtcgggattg ccgttcggcc cttccgggtt cacgtagatc aggcccatct
      481 gcaccgcgcc gagcggctcc gcaagttggc gctcgccgct atagcgttcg tcgccgagcc
      541 acgtgccctc gggaccccag taaagttctt cgggctccca gacatcggag cggccgccgg
      601 cgaaaccgaa ggtcttgaag cccatcgatt cgagagcgac gttgccggcg aggatcatca
      661 ggtcggccca ggaaattttc cggccgtact tctgcttgat tggccacagc aagcggcgcg
      721 ccttgtcgag gttcgcgtta tctggccagg agttgagcgg cgcgaagcgc tgctggccgg
      781 cgccggcgcc gccgcggccg tcgtgcaccc ggtaggtccc ggccgcgtgc cagctcatgc
      841 ggatcatcag cccgccgtag tggccgaagt cggccggcca ccaaggctgc gagtccgtca
      901 tcagggcacg cagatccttg atgacggcct tcaggtcgag acgcttgaac tccttggcgt
      961 agtcgaatgc ttcacccatc gggtcggaca ggttggagtt ccggtggaga accgaaagat
     1021 ccagcatatt cggccaccag tcgcgattgg agtgtccgtg ggtgaacggg caattgcccg
     1081 cgctcttgtc atcggttttt gcgtccattg ttctctcccg tcgtgttcag attttcacga
     1141 gttgtcggtg ggtgtgacct tgttaaagcg atcaggcaat aaggtctaat cgtattgatt
     1201 gggcctcgta ataagcctag cttattggcg tgatcacttt gcgccagttg cgttatttga
     1261 cctcgctcgc tcgccatagg catttcggaa gggcggcaga agattgcgca atcagtcagc
     1321 cggcgctgtc catgcaggtg cgcgaactcg agcgcgagat cggtgccgag ctggtggagc
     1381 gacggcccaa cgacatcgcg ctcaccgacg tcggccttga ggtcgtcgaa cgcgccgaac
     1441 gtattctcgc tgcgacccgc gacctggtcg actttgcgcg gcaccgcgat ctcctcagtg
     1501 ggcagctcaa gctcggcatt attccgacgc tggcgccgta cgtcctgcca cgtgtgctgc
     1561 cgcggctgca gtcgaaatat ccatatcttc ggctcgaagt ccgcgagacg caaaccaagg
     1621 ctttgctcga cgagcttgtg cgcggtgagc ttgactgcat catgctggcg cttcctgcgg
     1681 agagtacgga cgtcgaaact atgaagctgt tcgatgatcc gtttctgctg gccgtaccat
     1741 ccgcggacga gcacccgccg cgcggacgtg tcagtgtcga cgatgtcgat caacgccggc
     1801 tgatcctgct ggaggagggg cactgtctgc gcgaccaagc gctggcgttt tgtgcaacca
     1861 cgcggcgcga cgttcctgcc ggtctcggtg ccacctcgct tgcgaccgtc atgcagatgg
     1921 tggcgaatgg ctatggcgtg acgttggtgc ctgaagtcgc cgccgccgtc gaagtccgtg
     1981 acgaccgggt caaaatattg cggttcgctg caccggagcc cgcacgtacc atcggcttgg
     2041 catggcggcg aacctcgcca cgcaagacgg attttaccgc gtttagcgcg gtcgtgacgc
     2101 aaaccttgac gacaaatgcc tggcgcgcgc ccgcccgcaa ggcaaaataa aaagggcagc
     2161 ctttgcgctg cccttttgtg tcgatcttgg cggaggctta ttccgcagcg ccgccttggg
     2221 ctgcctgcgg ctgctcgcct ttgccttcga tgtcctcgcc ggtggcttga tcgaccgcct
     2281 tcatggacag gcggaccttg ccgcgttcat ccatgccgag cagcttcacc tttaccttgt
     2341 cgccttcctt gacgacgtcg gtcaccttgt tgacgcggcg cggcgcgagc tggctgatat
     2401 gcaccagtcc gtctttggca ccgaagaaat tcacgaaagc gccgaaatcc atgactttca
     2461 cgacggtccc ttcgtagatg tggccgactt ccggatcgga ggcgatcgat ttgatccagt
     2521 tgattgccgc cttgatagac tcgccgttcg ccgaggcgac cttcaccgat ccgtcgtcct
     2581 cgatgttgat cttggcgccg gtcttctcga cgatctcgcg gatcaccttg ccgccggtgc
     2641 cgatcacttc gcggatcttg tcggtcggaa tcttgaacat ctcgatgcgt ggggcgtgct
     2701 cgccgagttc ggcgcgggcg gcggtgagcg cctt
//
LOCUS       DASSRL010000404         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_728241, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000404 DASSRL010000000
VERSION     DASSRL010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728241"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1511
                     /locus_tag="VFL49_08225"
     CDS             297..1511
                     /locus_tag="VFL49_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912003.1"
                     /translation="MRPTRHVFTLALAGSLAAGFSLQALAQQTIKIGELNSYKAQPAF
                     LDPYKKGWEMAIEEINAKGGVLGKKLEVISRDDGANPGEAVRVAEELVTREGVSMIAG
                     TFLSHIGLAVTNFAGQKKVFFLAAEPLTDKITWQNGNKYTFRLRASTYMQVAMLMPAA
                     TGAKKKRWALVYPNFEYGQSAAENFKMLMKKAQPDVEFVTEQAPPLGKIDAGAVAQAI
                     DDAKPDAIFNVLFGGDLAKFVREGNTRGVFKNRTVVSLLSGEPEYLDPLKDEAPVGWI
                     VTGYPWDKIKTKEHMAFVDAYKKKYNDYPRLGSVVGYVTMHSLAAGIAKAKSTDTEKL
                     VEAFKGLKVDSPFGPFYYRASDHQATMGAYVGKIVLEGGKGTMADFKYVDGASVLPGD
                     AEVKKLRPVESN"
     gene            1572..3458
                     /locus_tag="VFL49_08230"
     CDS             1572..3458
                     /locus_tag="VFL49_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912004.1"
                     /translation="MTFSAFLFQALNGLSTASGLFFVAVGLSLIFGVSRIINIAHGSL
                     YMLGTYIAYSFATHIGGAFGFWGGIIATALIVGVIGMLIETGLLRRIYRAPELFQLLA
                     TFALVLIINDATLWLWGPEDLLGPRAPGLRGSIEVLGRRLPTYDVFLIVIGPLVLLAL
                     HFALARTRFGRLVRAATQDREMVSALGVNQALLFTAVFALGSALAGFGGALQIAREPA
                     NLATDLVAIGDAFVVVVVGGMGSITGAYLAAVIIAEVKAICIGIGVVDFGFVSVNFSK
                     LTLVAEFLVMAIVLIVRPYGLLGRPQGQVRSIAEPEDPIRPATPALKMLGAAVLALLL
                     LLPLLAQGSPYVLILGIDVLIAVIFATSLHFIMGPGGMHSFGHAAYFGLGAYGAALLV
                     KWLAVPMGLALVAAPIVALAGALLFGWFAVRLSGVYLAMLTLAFAQIVWALVFQWEGL
                     TGGSNGVIGVWPPPPFDKAWAYFLLSLVLAVIGVLMLRRFLFAPFGYAMRAGRDSPLR
                     AEAIGIDVKRVHWLGFAIAGAVCGVAGGLFAFAKGSISPETIHVGRSIDGLVMVLLGG
                     IQTLTGPIVGASVFATLQDTVMRQTEYWRALLGIIILLLVLAFPQGIVGAFASIWRKA
                     GAKS"
     gene            3455..>3661
                     /locus_tag="VFL49_08235"
     CDS             3455..>3661
                     /locus_tag="VFL49_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7912005.1"
                     /translation="MSVLAIRALSKAFGGVHAVNNVSFAIAEGEFLAMIGPNGAGKST
                     CFNMINGQLAPDSGEILFEGRNIAG"
BASE COUNT          624 a         1108 c         1151 g          778 t
ORIGIN      
        1 gaggtggcaa acctcgtcac catgcttgcg tccccgctga cggaaatggt caatggcacg
       61 atgatcgaga tcgacggcgg tcaggacaag ccgctgatgg accgtttccg cgacaagcca
      121 aaaggttagc tggcggtcaa aggaaagggt tcagcgacct tgcttcgctt gacaagttcg
      181 ggccgccggt cggactattt gcatacaaac aagctcgccc gccgcaggcg acagaatgtt
      241 gacaatcgcg gcgcgcgccg aaagcgcggt cagccgtcac gcagggagct tcaatcatga
      301 gaccgacgcg tcacgtgttc actcttgcgc tcgccggcag tctcgccgcc ggcttttcgc
      361 tccaggccct ggcgcagcag accatcaaga tcggcgaact gaacagctac aaggcgcagc
      421 ccgccttcct cgatccctac aagaagggat gggaaatggc gatcgaggag ataaacgcca
      481 aaggcggagt cctcggtaag aaactggaag tgatctcgcg cgacgacggt gccaatcccg
      541 gcgaagccgt gcgcgtcgcc gaggaactgg tgacgcgcga aggcgtctcc atgatcgcgg
      601 gtaccttcct gtcgcatatc gggctcgcgg tgacgaactt cgccggccag aagaaggtct
      661 tcttccttgc ggccgagccg ctcaccgaca agatcacctg gcagaacggc aacaaatata
      721 cattccgcct gcgcgcatcg acctacatgc aggtggccat gctgatgccg gcagcaaccg
      781 gcgcgaagaa aaagcgctgg gcactggtct atccgaattt cgagtacggc cagtcggcgg
      841 ccgagaactt caagatgctg atgaaaaaag cacagcctga cgtcgagttc gttaccgagc
      901 aggcgccccc gctcggcaag atcgacgccg gcgcggttgc gcaggcgatc gacgacgcca
      961 agcccgacgc gatcttcaac gtactgtttg gcggcgacct cgcgaagttc gtgcgcgagg
     1021 gcaacacgcg cggcgtgttc aagaaccgta ccgtggtgag cctgctctcc ggcgagcctg
     1081 aatatcttga tccgctgaag gacgaggcgc cggtcggctg gattgtcaca ggttatccct
     1141 gggacaagat caagaccaag gagcacatgg cttttgtcga cgcgtacaag aaaaaataca
     1201 acgactatcc gcgacttggt tccgtggtcg gctatgtgac gatgcactcg ctcgccgccg
     1261 gcattgccaa ggcgaaatcc accgataccg aaaaactggt tgaggccttc aagggattga
     1321 aggtggatag cccgtttggt ccattttatt accgcgccag cgatcatcag gcgacgatgg
     1381 gcgcctatgt cggcaagatc gttctggaag gcggcaaggg tacgatggcc gacttcaaat
     1441 atgtcgatgg cgcatcggtg ctgccgggtg acgccgaagt gaagaagctt cggcccgtcg
     1501 aatcgaacta ggttgcggtt gtgacatcct tcgctaacct ctccccgcct tgcggggaga
     1561 ggtgactttt tatgaccttc agcgcattcc tgtttcaggc gctcaacggg ctctccaccg
     1621 cctcaggtct gttctttgtc gcggtcggcc tgtcgctgat tttcggcgtc tcccgcatca
     1681 tcaacatcgc acatgggtct ctctacatgt tggggaccta cattgcgtat tcgttcgcca
     1741 cacatatcgg cggagcgttc ggattttggg gcgggatcat tgctaccgcg ctcattgtag
     1801 gcgtcatcgg catgctgatc gaaacagggc tgctgcgccg catctatcgg gcgccggaat
     1861 tgtttcagct gctcgcgacc ttcgcgctgg tgctgatcat caacgacgcg acactctggc
     1921 tatggggtcc cgaagatctg ctcggcccgc gcgcgccggg actgcgcggc tcgatcgagg
     1981 tgctgggccg ccggctgccg acctatgacg tgttcctgat cgtgatcggc cctctggtgc
     2041 tgctggcgct gcattttgcg ctcgcccgca cgcgctttgg ccgcctggtg cgggcggcca
     2101 cgcaggaccg ggaaatggtg agcgcgctcg gcgtcaacca ggcactgctg ttcacggcgg
     2161 tgttcgctct tggctccgcg cttgccggtt tcggcggcgc cttgcagatc gcgcgtgagc
     2221 cagcaaacct cgccacagat ctggttgcga tcggcgacgc cttcgtggtg gttgtcgtcg
     2281 gcggcatggg gtcgatcacc ggcgcctatc tcgcagccgt tatcatcgcc gaggtgaaag
     2341 cgatctgcat cggcatcggc gtggtcgact tcggcttcgt cagcgttaat ttctcaaagc
     2401 tgacgctggt cgcggaattc ctcgttatgg cgatcgtgct gatcgtccgc ccctatggcc
     2461 tgctcggccg cccgcaaggc caggtccgtt cgatcgccga gccggaagat ccgatccggc
     2521 cagccacacc ggcattgaag atgctcggcg ctgcggttct ggcgcttttg ttgctcctgc
     2581 cgctgctcgc gcaaggctcg ccttacgtgc ttattctcgg catagacgtg ctgatcgccg
     2641 tcatcttcgc gacctcgctg catttcatca tgggtccggg cggtatgcat tcgttcggcc
     2701 atgccgccta tttcgggctt ggcgcctacg gcgctgcgct gctggtgaaa tggctggcgg
     2761 tgccgatggg tcttgcattg gtggcggcgc ccatcgtcgc gcttgccggg gcgctgttgt
     2821 tcggctggtt cgcggtgcgg ctgtcgggtg tgtatctcgc gatgctgacg ctcgcctttg
     2881 cgcagatcgt ctgggcactg gtcttccagt gggaagggct gaccggcggc tcgaatggcg
     2941 tcatcggcgt atggccgcca ccgcctttcg acaaggcctg ggcctatttc ctgctgagcc
     3001 tggtgctcgc cgtgatcggc gtgctgatgc tgcggcgctt tctgttcgcg cccttcggtt
     3061 acgccatgcg cgccggccgc gattccccgc tacgcgccga agccatcggc atcgatgtca
     3121 agcgggtgca ttggcttggc tttgcaatag cgggtgcggt ctgcggtgtc gccggtggcc
     3181 tctttgcctt tgccaagggc tcgatctcgc cggagacaat tcatgtcgga cgttccatcg
     3241 atggtctggt gatggtgttg ctcggcggca tccaaacgct gaccggcccg atcgtcggtg
     3301 cttcggtctt tgccacgctg caggatacgg tcatgcgcca gaccgagtac tggcgcgcct
     3361 tgctcggcat catcatcctg cttctcgttc tggctttccc gcagggcatt gttggcgcct
     3421 tcgcgtcgat ctggcgcaaa gcgggagcga agtcgtgagc gtgctcgcca tccgcgcgct
     3481 gtccaaggca ttcggcggcg tccacgccgt caacaatgtc agcttcgcga tcgcggaggg
     3541 cgaattcctt gccatgatcg ggcccaacgg tgccgggaag tcgacctgct tcaacatgat
     3601 caacgggcag ctcgcgcccg attccggaga aattcttttc gaaggccgta acattgcggg
     3661 c
//
LOCUS       DASSRL010000405         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1533229, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000405 DASSRL010000000
VERSION     DASSRL010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533229"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="ftsY"
                     /locus_tag="VFL49_08240"
     CDS             <1..729
                     /gene="ftsY"
                     /locus_tag="VFL49_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7912006.1"
                     /translation="EGRYPQGISPDEVKAVLAGEIEKTLAPVARPLELTTRPFVILVA
                     GVNGSGKTTTIGKLAARFRADGKTVMLVAGDTFRAAAIDQLKIWGGRTEAKVIAGEPG
                     SDAASLAFDAISAAKSEGVDVVLVDTAGRLQNRVELMGELEKMVRVMRKVEPTAPHAV
                     LLVLDATVGQNALSQVEIFGKTAGVTGLVMTKLDGTARGGILVAIAEKFGLPVHFIGV
                     GESVDDLAPFTARDFSRAVVGLEE"
     gene            738..1298
                     /locus_tag="VFL49_08245"
                     /pseudo
     CDS             738..1298
                     /locus_tag="VFL49_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
     gene            1313..1783
                     /locus_tag="VFL49_08250"
     CDS             1313..1783
                     /locus_tag="VFL49_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HET7912007.1"
                     /translation="MNFSRVWKSIAAGLCGSAAHTGLMALKSWARILPSFEPYGDLQV
                     LLASLVGSSVNPVIPWVLSYFNGSVVLAFLFGQSYSRLPGRTPLAKGTVFGLAIWIVM
                     GLTFFPVIGKGPFATDAGLGLKPTIFSLLMVLTYSVTLSTVYSIFGLPKQASTS"
     gene            complement(1785..2372)
                     /locus_tag="VFL49_08255"
     CDS             complement(1785..2372)
                     /locus_tag="VFL49_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET7912008.1"
                     /translation="MTTASSEGSARRRRNVLVFVPLVAFAALAALFFYRLGAGDPSRL
                     PSALIGHPIPATNLPPLPGLERDGKPVPGLTNATFEGAVTVVNVWASWCVPCHDEVPF
                     LEQISKDQRIQLVGINYKDAPDNARRFLNRYGNPFAAAGRDEAGRASIDWGVYGVPET
                     FLVGRDGRIAYKLVGPITADNLARTLKPEIEKALR"
     gene            complement(2377..2544)
                     /gene="ccmD"
                     /locus_tag="VFL49_08260"
     CDS             complement(2377..2544)
                     /gene="ccmD"
                     /locus_tag="VFL49_08260"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HET7912009.1"
                     /translation="MNLGPHADFIVAAYAVTLFVVAALIAWVLVDYLAQRRILDGLEE
                     RGITRRSRRKG"
     gene            complement(2541..3272)
                     /locus_tag="VFL49_08265"
     CDS             complement(2541..3272)
                     /locus_tag="VFL49_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HET7912010.1"
                     /translation="MAIIDFTNPTRFLRIADRVLPWLVAATAVLFAVGLYRALNAPDD
                     YQQGATVKIMFLHVPAAWLGMFGWALMSVAALGTLVWRHPLADVAGKAAAPIGAAFTF
                     ICLVTGSLWGRPTWGTYWVWDARLTSMLVLLLLYLGVLALYWTAEEPGRGARAAAILS
                     LVGAVNLPIIKFSVDWWNTLHQPASVFRIGGATIDPSLLTPLLIMALAFTLLFVTLHL
                     AVMRNEILRRRVRSMRLMQAQELHA"
     gene            complement(3291..>3825)
                     /gene="ccmB"
                     /locus_tag="VFL49_08270"
     CDS             complement(3291..>3825)
                     /gene="ccmB"
                     /locus_tag="VFL49_08270"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET7912011.1"
                     /translation="LLARIGPAVLWLGALLASLLALDRLFATDHDDGSLDLLMMGGVP
                     LELTVAAKALAHWLTTGLPLVLIAPLLGLMLNVDMHALGWLALTLAAGTPALTFIGLI
                     GAALSVALRRGGLLLAVLVLPLTVPVLIFGVAAANAAIAGPTPFGPAFTILCALSLAS
                     LVLGPIAAAAALRHGLE"
BASE COUNT          768 a         1127 c         1212 g          718 t
ORIGIN      
        1 gagggacgtt atccgcaagg aatttctccc gacgaggtta aagcagttct ggcgggtgag
       61 attgagaaga cgctggcccc ggttgcgagg ccgctggaat tgaccacacg gccatttgtc
      121 atactggtag ccggcgtcaa cggttccgga aagacgacga cgatcggcaa gctcgccgcg
      181 cgtttccgcg cggacggaaa gacggtgatg ctggtcgcgg gcgatacctt ccgtgctgcg
      241 gcgatcgatc agctgaagat ctggggcgga cgaacggaag caaaggtgat tgccggcgag
      301 cccggcagcg acgcagcgag ccttgccttt gacgcgatct ctgctgcgaa gagtgagggt
      361 gtggacgtgg tgctcgtcga taccgccggc cgcctccaga atcgcgtcga gttgatgggc
      421 gaactggaaa aaatggtgcg cgtcatgcgt aaggtcgagc cgacggcacc gcatgcggtg
      481 ctgcttgtgc tcgatgcgac ggttggtcag aacgcactca gccaggtcga aatttttggc
      541 aagaccgcgg gggttaccgg tctggtcatg accaaactgg acggaacggc acgcggcggc
      601 attctggtgg ccatcgccga gaaattcgga ctgccggtgc atttcatcgg cgtcggcgaa
      661 agcgtggatg atcttgcgcc atttaccgcg cgggatttct cgcgcgccgt ggtaggactg
      721 gaagagtgag aacggtcgtg gccgcgcaaa agaagcagct caaccctgtc ctcaagctgg
      781 tgctcgatat tgggccgctg gttcttttct ttgcggccaa cgcgaagttc gggatatttg
      841 tcgcgaccgg cgccttcatg gtggctgtac tggctgcgct ggcggtttcc tatgcgatga
      901 cccggcatat tgcggtcatg ccagtggtca cagccgtcat tgtgctgatc ttcggcggac
      961 tgacgctggt gctgcacgat taactgttca tcaagctcaa gccaacgatc atttacgttc
     1021 tgttcggcgg cacgctgatg ggcggtcttc tgttcggaaa gcctttgctc gggattgtgt
     1081 tcgattccgt attccatctg cgggacgaag gctggcgcaa gctgacttgg cgatgggcgc
     1141 tgtttttctt cgcccttgcg atcctgaacg agatcgtctg gcgcacgcag accaccgact
     1201 tctgggtgag cttcaaattg tttggcgtgg tgccactgac ttttctgttc gcagcgctgc
     1261 aatatccgct gctcacaaaa tacagtgtcg ataaataatc ctgacaagag ctatgaattt
     1321 ttctcgtgtc tggaaatcga ttgcggcggg cttgtgcgga agtgctgcgc ataccgggct
     1381 tatggcgctg aaatcctggg cccgaatact tccaagcttt gagccctatg gcgatttgca
     1441 ggtcttgctg gcttcgctgg tcggaagttc cgttaacccg gtgatcccct gggtgctgtc
     1501 gtacttcaac ggctcggtag tcctcgcgtt tctgttcggc caatcctatt cccgactgcc
     1561 cggccgcacc ccgttggcta agggaacggt attcggcctg gccatttgga tcgtgatggg
     1621 gttgacgttt ttccccgtta tcggcaaggg gcccttcgca acagacgccg gactgggcct
     1681 caagccaacg attttttcgt tgcttatggt tcttacctac agcgtgacct tgagcacggt
     1741 gtactcgata ttcgggttac cgaagcaggc gtcaacatct tagactagcg caacgccttt
     1801 tcgatttccg gttttaacgt ccgggcgagg ttgtccgccg tgatcggccc taccagtttg
     1861 taggcaatgc ggccgtcgcg gccgacaaga aacgtctccg gtaccccgta gacaccccag
     1921 tcgatcgacg cgcggcccgc ttcgtcgcgg ccggcggcgg caaaaggatt accatagcgg
     1981 ttgaggaacc gccgtgcgtt gtccggtgcg tccttgtagt tgatgccgac gagttggatg
     2041 cgctgatcct tcgagatctg ctccagaaac ggcacctcgt cgtgacaggg aacgcaccac
     2101 gacgcccaca cattcaccac cgttaccgcg ccctcaaatg tcgcgttggt aaggccgggc
     2161 acgggcttgc cgtcgcgttc aagacccggc agcggcggca gattggttgc gggaatcgga
     2221 tgtccgatca atgccgacgg aaggcgggac ggatcgccgg cgcccagacg gtagaaaaac
     2281 agcgctgcca gcgcggcaaa cgcaaccagc ggcacaaaca cgagaacatt gcggcgcctt
     2341 ctggccgagc cttcactcga ggcggtagtc attcgcctag cccttgcgcc gggagcgccg
     2401 ggttatcccg cgctcttcga ggccatcgag aatgcggcgc tgcgccaagt agtcgaccag
     2461 cacccacgcg atcagtgccg ccaccacgaa caaggtcacc gcgtaagcgg caacgatgaa
     2521 atcggcatgc ggcccgaggt tcatgcgtgc agctcctgcg cctgcatgag acgcatgctg
     2581 cgtactcgcc gcctcagaat ctcgttgcgc atgaccgcaa gatgcagcgt gacaaagagg
     2641 agcgtgaatg ccagagccat gatcagcagc ggagtgagca acgaggggtc gatcgttgca
     2701 ccgccaatgc ggaagacgga agccggctgg tgcagtgtgt tccaccagtc gacggagaat
     2761 ttgatgatcg gaagattgac ggccccgacc agcgacagga tagcggcggc gcgcgcgccg
     2821 cggcccggct cttccgcggt ccagtacagc gcaagcacgc cgagatagag cagcaacagc
     2881 accagcatcg atgtcagtcg cgcatcccag acccaataag tgccccacgt cggacgtccc
     2941 cagagcgagc cggtgacgag gcagatgaac gtgaacgcgg cgccgatcgg cgcggcggcc
     3001 ttgccggcaa cgtcggcaag cggatgccgc cacaccaatg tgccgagcgc ggccacgctc
     3061 atcagcgccc agccgaacat gccaagccat gccgccggaa cgtgcaggaa catgatcttc
     3121 acggttgccc cctgctggta gtcgtccggc gcattaaggg cacgatagag accgaccgcg
     3181 aacaggactg cggtggccgc gaccagccac ggcaatacac ggtcagcgat gcgcaggaag
     3241 cgcgtcgggt tggtgaaatc aattatagcc atctgcaatc gctcagcatc tcattccagt
     3301 ccatggcgca aggcggcggc tgcggcaata gggccaagca ccaggcttgc caagcttagc
     3361 gcacaaagaa tggtgaaagc ggggccgaac ggcgtggggc cggcgatcgc ggcattcgcg
     3421 gctgccacgc cgaagatcag cactggcaca gtaagcggca acaccagtac ggcgagcagc
     3481 aatccgccgc gtcgcagcgc cacggatagc gcagcgccga taagcccgat gaacgtgagg
     3541 gcaggcgtgc ccgccgccaa tgtcagcgcg agccacccga gcgcatgcat gtcgacgttg
     3601 agcatgagcc cgagcagggg cgcgatcagt acaagcggca atccggtggt gagccagtga
     3661 gcaagcgctt tcgccgccac cgtcagttcg agcggaaccc cacccatcat aaggagatcg
     3721 agcgatccat cgtcgtggtc ggtggcaaac aggcgatcga gcgccagcag actcgccagc
     3781 aacgcgccga gccacagaac tgccggcccg atccgggcaa gcagc
//
LOCUS       DASSRL010000406         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_325269, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000406 DASSRL010000000
VERSION     DASSRL010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325269"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1784
                     /locus_tag="VFL49_08275"
     CDS             66..1784
                     /locus_tag="VFL49_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7912012.1"
                     /translation="MVESLDMLRLVSRAAGERRAVAWRSGKPLTAQAFVRRVGAWRER
                     LESSGAAFALYHTDAVEFAAALFGGWHGSKKIYLPADNLPGTCEGLHSKVDGYLGEFE
                     PVWNPVNLPEECGTDGATDFARLDGDFPGLVLYTSGTTGAAQPIEKKLTQMAAEVSTL
                     EATFGALLGTAEILSTVSHQHIYGLLFNVLWPLTAGRALHARTLAFIEELATVDRECA
                     LVSSPAHLKRLPESPLWASIAKRLRAVFSSGGPLPLEVAQETRRLLGHMPMEVYGSSE
                     TGGIAWRRQHAESGIAWTPFCGADWRIDPTERVLEVRSPNLPDREWFRTADLATSVAD
                     GQFILNGRIDRIAKIEGKRISLKAIEGLLSSSPLVSSVRALALEGARQRIAVFIVPSD
                     EGRCRLASCGRRGVNRVLRALLSNSIEPVALPRLWRYLDALPTNAQGKITHLALMASL
                     EREPAVQTTPCRRLVQKSAHRAVFQLTGPSNLLYFDGHFPGQPILPGVVQLDWVIRIG
                     RECFELPAEFRAVRALKFRRVIPPNLPFTLELDYRPDTGMLDFKILSDFGNHASGRVV
                     FGPADV"
     gene            1804..>2168
                     /locus_tag="VFL49_08280"
     CDS             1804..>2168
                     /locus_tag="VFL49_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7912013.1"
                     /translation="MYNHEQAVGAVVENVLAHHLPCILVDDGSETKCAQELARLAAAA
                     PEQITLLRHPVNRGKGSAVLTGVRRAGQAGYSHALQIDADGQHRAADIPRFLAQAAAR
                     PQSLIIGCPEYDLSAPVLRL"
BASE COUNT          371 a          650 c          674 g          473 t
ORIGIN      
        1 tgttttgatc ggacttcttt ttgtcggcga gtattgcgtg cgtcgccgtt tcacgcgtcg
       61 cctccatggt tgaaagtctc gacatgctga ggctcgtaag ccgcgcggcc ggtgaacgtc
      121 gcgctgttgc ttggcgttcg ggaaagcccc tgaccgcgca agcatttgtc cggcgggtcg
      181 gtgcgtggcg cgagcggctc gagagcagcg gtgctgcctt cgctctttat catacggatg
      241 ctgtggagtt tgctgccgct cttttcggcg gctggcatgg cagcaagaag atttatttgc
      301 cggcagataa tctgccgggg acttgcgagg ggctgcactc aaaggtcgac ggctatttgg
      361 gcgaattcga gccggtctgg aatccggtga atctgcctga agaatgtggt accgacggag
      421 cgactgactt cgcgcgactg gatggcgatt ttcctggttt ggtgctctac acttccggca
      481 ccaccggcgc cgcgcagccg atcgagaaaa aactcactca aatggccgcc gaggtttcga
      541 ccctcgaagc gacatttggc gccctgcttg gcacggcaga aatcctctct acggtttcgc
      601 atcaacatat ttacgggctc ctgttcaatg ttctttggcc gcttaccgca ggacgcgcgc
      661 tccacgcgcg gacgctcgcc ttcatcgaag agctggcgac tgtagatcgc gagtgcgcac
      721 tggtgtcaag cccggcacat ctcaagcgtt tgccagaaag tcccctttgg gcgtcaatcg
      781 cgaagcggct gcgcgcggta ttttcctccg gtggaccttt gccgctcgaa gtcgcccaag
      841 aaactaggcg actgctcgga catatgccca tggaagtgta cggcagctcg gaaaccggcg
      901 gcatcgcttg gcggcggcag cacgctgaaa gcggtatcgc atggacgccc ttttgcggcg
      961 cggactggcg catcgatcca accgagcgcg tgctcgaggt gcgctcgccg aatctgccgg
     1021 accgggaatg gtttcgcacc gcggatctcg cgacctccgt cgctgatggc cagtttattc
     1081 tcaacggtcg gattgatcgg atcgccaaaa tagaaggcaa gcgaatttcg ctgaaggcca
     1141 tcgaagggct cctatcctcc tcgccgctgg tttcgagcgt tcgcgcgctc gcgcttgaag
     1201 gggcgcgcca acggatcgcg gtcttcatcg tgccttccga cgaaggccgc tgccggttag
     1261 cgtcttgcgg caggcgcggc gtcaatcgtg tgctccgtgc attgctgagt aattctatcg
     1321 aaccggtggc tttgccgcgg ctctggcgtt atctcgacgc gctgccgacg aatgctcagg
     1381 gcaagatcac acacctcgcg ctgatggcat cactcgagcg cgagcccgcg gttcagacga
     1441 caccctgccg ccgtctcgtt caaaaatccg cgcaccgcgc ggttttccaa ttgaccggcc
     1501 ccagcaatct gctttatttc gacggccact ttcccggcca gccgatactt cccggcgtcg
     1561 tccaattgga ttgggtgatc cggatcgggc gggagtgttt cgagttgccg gctgagtttc
     1621 gcgccgtacg cgcgctcaag ttccgccggg tgatcccgcc gaatctgccg ttcacgcttg
     1681 agctcgatta tcggccggat acgggcatgt tggatttcaa aattctttcg gattttggca
     1741 accatgcgag cgggcgcgtg gtgttcggac cggccgatgt ttaagccgtg cgcggtgatc
     1801 ccggtgtaca accacgagca ggcggtgggt gccgtcgtcg aaaacgtgct tgcgcatcat
     1861 ttgccctgta tcctcgtcga tgacgggagc gaaacgaagt gcgcgcaaga gctcgcgcga
     1921 ttagccgccg ccgcaccaga gcaaattact ctgctgcgcc atccagtcaa tcgcggcaag
     1981 ggcagtgccg tgttgaccgg ggtacgccgt gcgggccagg caggctacag ccatgcattg
     2041 caaatcgacg ccgacggtca acaccgtgcg gccgatatcc cgcgctttct tgctcaagcg
     2101 gcggcgcggc cgcagtcgtt gattatcggc tgtcccgaat atgatctgag cgcgcccgtg
     2161 ctgcggct
//
LOCUS       DASSRL010000407         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1533246, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000407 DASSRL010000000
VERSION     DASSRL010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533246"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFL49_08285"
     CDS             <1..183
                     /locus_tag="VFL49_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912014.1"
                     /translation="KKRAEIFRKWFLKIFIGKACGRERGLENIYGAGNLLTKFDTDLP
                     CVLVPRCKVGALGVKQ"
     gene            complement(621..1808)
                     /locus_tag="VFL49_08290"
     CDS             complement(621..1808)
                     /locus_tag="VFL49_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7912015.1"
                     /translation="MDAKLEELSFTYFPENYRWSHGLLIGLNMAPWGGAEIGEVNRVG
                     LRLKKQLGDDDAWFREWAREARKAEDAGRQRIVEGFTTSGAQYLHRASAYYHVGERFL
                     QPKSKEGLDAYMRGVACFRDAAKYIKRPRMEHVEIPYGGTSLPAVYVHAEPANASRKV
                     PAVVFFDGLDVTKEIQYFKGVADLVARGIACLIVDGPGNGESIRFRNLYLRHDTEHYA
                     TPVFEYLAARPEVDAKRIGVMAISLGGYYAPRAAAFEKRYACCLAWGAQWDYQKIWRD
                     RFERLARADTPSLSVAHQHIAWVLNVKTQDDVLKHLEPFKLDGVVQKISCPFLMLHGD
                     GDEQIPLSEAQKCFDAVGSKQKTMKIFTREEGGYHHCQIDNQSVCAAYMWDWLEQVLE
                     PAR"
BASE COUNT          419 a          629 c          667 g          471 t
ORIGIN      
        1 aaaaagcgag cggagatatt ccgcaaatgg ttcctcaaga ttttcatcgg caaagcgtgc
       61 ggtcgcgagc gaggcctcga aaatatctat ggggccggga atctgctgac gaagttcgac
      121 acggatttgc cttgcgtact cgttccacgg tgcaaagtcg gggccctagg agtgaagcaa
      181 taggatgcgt tttggctcgg cggcattcgc tgcgaccgtc gcgatcacga ggaaaatgag
      241 gatgttgatc cagcctgatc ttttgcaatg ggcggtaagg cgcggttttc cttcgatacg
      301 agttggttta ctgcagcgat tggtcaatgg cgctcctggg aatgatctgc acattgacaa
      361 tacacttgcg tacagaaggt cggaaattgt tgtaccacct ttatacgttg tgagccggtt
      421 gtcttacaga tgcattcacc agggtcgaaa aaagctgcga caaggagggc aaatattgcc
      481 cacttctctc atcggtcggt ttgggtcttg ctctttgagc gctattcccg cttgccggct
      541 cgtacggctc ttcacggaat cggcgcctag gccatgggat tcgagaacga ggcggaccaa
      601 tcatcccagg atagatgcgc ttagcgtgcg ggctccagca cctgctcgag ccagtcccac
      661 atataggccg cgcagacgct ctggttgtcg atctggcaat ggtgatagcc gccctcttcg
      721 cgcgtgaaga tcttcatggt cttctgcttc gagccgacgg catcgaagca cttctgcgct
      781 tcgctcaggg gaatctgctc gtcgccgtcg ccgtgcaaca tcaggaacgg acaggaaatt
      841 ttctgcacga cgccgtcgag cttgaacggc tcgaggtgtt tgagcacgtc atcctgagtt
      901 ttcacattga gcacccaggc gatgtgctgg tgtgcgaccg agagcgacgg cgtgtcggcg
      961 cgcgcgaggc gctcgaagcg gtcgcgccag atcttctgat agtcccactg cgcgccccag
     1021 gcgaggcagc acgcgtagcg tttctcgaac gccgccgcgc gcggcgcgta gtagccgccg
     1081 aggctgatcg ccatcacgcc gatacgtttt gcgtccactt ccggccgcgc ggccaggtat
     1141 tcaaagaccg gtgtcgcgta gtgctcggtg tcgtggcgca gatagagatt gcggaagcgg
     1201 atgctttcgc cgttgccggg gccgtcgacg atgagacagg caatgccgcg cgcgaccagg
     1261 tcggcgacac ccttgaagta ctgaatttct ttggtgacat cgagtccgtc gaagaacacg
     1321 acggccggca ccttgcggct cgcgttcgcc ggctccgcgt gcacgtaaac ggccggcagg
     1381 ctcgtgccgc cgtagggaat ttccacgtgc tccatgcgcg ggcgcttgat gtatttcgca
     1441 gcgtcgcgga agcacgcaac gccgcgcata taggcatcga gtccctcttt gctcttcggc
     1501 tgcaggaagc gctcgccgac gtggtagtag gcgctggcgc ggtggagata ttgcgcgccg
     1561 ctggtcgtaa agccttcgac aatgcgttgg cggccggcgt cttccgcctt gcgcgcctcg
     1621 cgcgcccatt cgcggaacca cgcatcatca tcgccaagct gctttttcag ccgcagcccg
     1681 acgcggttga cctcgccgat ctcggcgccg ccccagggcg ccatgttgag gccaatcagc
     1741 aggccgtgcg accaacggta gttttcgggg aaataggtga aggatagttc ttcaagcttg
     1801 gcatccatgc gaggagactc cgggcggacc aggttttgcg ttgcccaagc taaccccagc
     1861 cagcggaatt ctgaagcacg accctatagt ggtatggtgg catttcgtca gcctggcgac
     1921 gtcgtaggca ttgccaagaa taaaagcgca attcgtcacg ccgttttcag tgaggcgccg
     1981 cggcgcaacc tccagcatga cctggtcggt attgatcgcg tctgcttgcg cacagcatca
     2041 aagaaagttt cgaaaccgcc gagattgcct cgtgcttatc aacagcccgc gagtacgtgc
     2101 gtttagcttt cgctgcccaa ccgcccgcga atactctcag ccgatgattt tctgggcaca
     2161 ttgagcttgc gcatcagagc gctggg
//
LOCUS       DASSRL010000408         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_850621, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000408 DASSRL010000000
VERSION     DASSRL010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850621"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFL49_08295"
     CDS             <1..876
                     /locus_tag="VFL49_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HET7912016.1"
                     /translation="LPDRGVVKVVGDDARRFLNGLVTGDMAKVTAGNPRFTALLTPQG
                     KIIVDFIIAEAPAEDGGGFFLDCPRALALALVEKLNFYKLRAKVICEDLSEVLGVMAV
                     WKGAADSEYGLSYPDPRLPGLGSRIMLPPHLAAAAAADIGADFTDADNYDAHRIALGV
                     PRGGLDFIYGDTFPHEADMDQLNGVDFDKGCYVGQEVVSRVEHRASARSRVVPIAYDE
                     FAPSAGLPIMAGEKQVGTLGSTAKGRGLALLRLDRVEDALAAKTTLEAGGVAVRPVKP
                     SWAKFDWPGEAKAIS"
     gene            873..1478
                     /locus_tag="VFL49_08300"
     CDS             873..1478
                     /locus_tag="VFL49_08300"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET7912017.1"
                     /translation="MSKVILHADGFARCPWPKQDPLYVAYHDDEWGVPEYDDRALFEK
                     LVLDGFQAGLSWITILRKRDNFRRAFNEFDPEKIARYPKRKVESLMKDAGIVRNRAKV
                     EGAVSSARAWLKIMEGPGFSNFLWDHLDGRPLQNRFTSTRQVPAETPLSKQISKELVA
                     QGFKFVGPTIVYAFMQAVGMVNDHLVTCHRHAAVSKLARKR"
     gene            1475..2110
                     /locus_tag="VFL49_08305"
     CDS             1475..2110
                     /locus_tag="VFL49_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HET7912018.1"
                     /translation="MSKPATARASKTPARAWQRMLSGRRLDLLDPSPLDVEVDDIAHG
                     LARVARWNGQTQGGHIFSVAQHSLLVEALARARVPRLDPKGRLAILLHDAPEYVIGDM
                     ISPFKAVIGDSYKAVEKRLLGAIHLRFGLPAKLPEPLQALIKAADRSAAYLEATRLAG
                     FAEAEARKFFGPPPKFSAVLERDYLKPWPAGTAQSRYRERFEKLLRRLSFL"
     gene            2185..2691
                     /locus_tag="VFL49_08310"
     CDS             2185..2691
                     /locus_tag="VFL49_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HET7912019.1"
                     /translation="MIHVCSLSRLHATIDETGARHVVTLLRLVDRVQRPSHIAPENHL
                     VLSVDDITMPMDGYTLPAHDHVQRLIKFVTTWDRKTPMVMHCFAGISRSTAAAYVAAC
                     ALNPQRDEMQIAWDIRRSSRTAQPNSRIVSIADRVLNRDGRMIRAIDAIGLGDIAAEG
                     HPFRLDVL"
     gene            complement(2707..2967)
                     /locus_tag="VFL49_08315"
     CDS             complement(2707..2967)
                     /locus_tag="VFL49_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912020.1"
                     /translation="MKMRCFALAISALVAMPVIASAQGVVGGAQEGASKGAREGNRAA
                     GPVGGAVGTVVGGTVGGVTGGVKGALGVSDKPKSKSKKQTNN"
     gene            3174..>3495
                     /locus_tag="VFL49_08320"
     CDS             3174..>3495
                     /locus_tag="VFL49_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912021.1"
                     /translation="MAEIIGLVLLILAFPVISVIALVKALNTRDSVRRLELRIAVLEA
                     GPARRPGEATAPEPVPPVAVEQPTIAEPVAAPETPEPAAAPVAPAPASTTLEERFGTQ
                     WVVWI"
BASE COUNT          652 a         1142 c         1072 g          629 t
ORIGIN      
        1 ctccccgatc gcggcgtggt caaggttgta ggcgatgacg cccgacgctt tctcaacggc
       61 ctggtaaccg gcgacatggc caaagtcacg gccggtaacc cgcgtttcac ggcgttgctc
      121 acgccgcagg gaaagatcat cgtcgacttt atcattgcgg aggccccggc tgaggacggc
      181 ggcggtttct tccttgactg cccgcgggca ctcgccctgg cgctggtcga aaaattgaat
      241 ttctacaaat tgagggcaaa ggtgatctgc gaagatctgt cagaagttct tggcgtcatg
      301 gcggtatgga aaggcgcggc cgacagcgaa tatgggctga gctacccgga cccgcggctg
      361 ccgggactcg gctcgcgcat tatgctgccg ccgcatcttg cggccgcagc cgcggcagat
      421 atcggcgccg acttcaccga tgccgacaac tatgatgcgc atcgcattgc gctcggcgtg
      481 ccgcgcggcg ggctcgactt catctacggc gacacctttc cgcacgaagc cgacatggac
      541 cagctcaacg gtgtcgattt cgacaagggc tgctatgtcg gccaggaggt tgtttcgcgg
      601 gtcgagcacc gtgccagcgc gcgcagccgc gtggtgccga tcgcctacga cgaatttgcg
      661 ccgagcgcgg gcctgccgat catggccggc gagaaacagg tcggcacgct cggctcgacc
      721 gcgaaaggcc ggggccttgc gctcctgcgg cttgaccgcg tggaagacgc cttggccgcg
      781 aaaacgacgc tcgaagcggg tggcgtcgcc gtccggcccg tgaagccatc ctgggcaaaa
      841 ttcgactggc cgggcgaggc aaaagcgatc tcatgagcaa ggttattctc cacgccgacg
      901 gattcgcgcg ctgcccctgg ccaaagcagg atccgctcta cgtcgcctat cacgacgatg
      961 aatggggcgt tcccgaatac gacgaccgcg cactgtttga aaagcttgtg ctcgacggct
     1021 tccaggccgg cctgtcgtgg atcaccatcc tgcgcaaacg cgacaacttc cgccgtgcgt
     1081 ttaatgaatt cgatccggaa aaaatcgcgc gctatccgaa acgcaaggtc gaatcgctga
     1141 tgaaagatgc cggcatcgtg cgcaaccgcg cgaaggtcga aggcgcagtg tcgtccgcgc
     1201 gggcctggct gaaaatcatg gagggtccgg gcttctcgaa tttcctgtgg gatcacctcg
     1261 acggcaggcc gctgcaaaac aggttcacct cgaccaggca ggtgccggcg gaaacaccgc
     1321 tgtcgaaaca aatatcgaaa gaactggttg cgcagggctt caagttcgtc ggccccacaa
     1381 tcgtctacgc cttcatgcag gccgtcggca tggtcaacga tcatctcgtg acctgccacc
     1441 gtcacgcggc tgtctcaaag ctcgcccgga aacgatgagc aaaccggcca ccgcgcgcgc
     1501 gtcgaaaacg ccggcccgcg cgtggcagcg catgctgtca ggccggcgcc tcgatctgct
     1561 tgacccctcg ccgctcgacg tcgaggtcga cgacatcgcg catggcctgg cacgggtcgc
     1621 gcgctggaac ggtcagacgc agggcggcca tattttttcc gtcgcgcaac actcactgct
     1681 tgtcgaagcg ctggcgcgcg ccagagttcc gcggctcgac cccaagggcc gccttgcgat
     1741 cctgctgcac gatgcgccgg aatacgtcat cggcgacatg atctccccgt tcaaggcggt
     1801 gatcggcgac agctacaagg cggtggagaa gcggctgctc ggcgcgatcc atctgcgctt
     1861 cggcctgccg gcgaagctgc cggagccgct gcaggcgctc atcaaggcgg cagacagaag
     1921 cgccgcctat ctcgaggcga cgcggcttgc gggcttcgcg gaagccgagg ctcggaagtt
     1981 tttcggcccg ccgccgaaat tctccgccgt tctcgagcgc gactatctca agccgtggcc
     2041 cgccggaacc gcgcaatccc gctaccggga gcgcttcgaa aagctgctcc ggcgcctgag
     2101 ctttctctga gcccctgttt tttaaccttt tcttggcgcg atccagggcc tagataaggg
     2161 ccgggaaatc gcacgaacga tcgcatgatt cacgtctgct cactttcacg cctgcacgcg
     2221 acgatcgacg agaccggcgc caggcacgtt gtcaccttgc tgcgcctcgt cgaccgggtt
     2281 cagcggccaa gtcacatcgc gccggagaac cacttggttc tctccgtcga cgacatcacg
     2341 atgccgatgg acggttatac gctgcccgca cacgatcacg tgcagcggct gatcaaattc
     2401 gtcacgacct gggaccgcaa gacgccgatg gtgatgcact gcttcgccgg catcagccgg
     2461 tcgacggccg cggcttatgt ggcagcctgc gcactcaacc cgcagcgcga cgagatgcag
     2521 atagcctggg acatcaggcg ttcctcgcgc acggcgcaac caaattcacg catcgtctcg
     2581 atcgctgatc gcgtgctcaa ccgcgacggc cgcatgatcc gcgcaatcga cgccatcggg
     2641 ctcggcgaca tcgcagccga aggtcacccg ttccggcttg atgtgctctg aattcggatt
     2701 agtgcgtcag ttgttggttt gcttcttcga tttgctcttt ggcttatcgg aaacgcccag
     2761 cgctcccttc acgcctcctg taacgccgcc gacagtgccg ccaacgacgg taccaaccgc
     2821 gccaccgacg gggcccgccg ccctgttgcc ttcccgcgct cccttgcttg cgccttcctg
     2881 agcgccacca acgactcctt gcgcgctggc aatcacaggc atggccacga gagcagaaat
     2941 ggcaagagcg aagcatcgca ttttcatgtg cgccctccga aagaaattga aaattcagcc
     3001 accgaataaa gcctgaaacc cgccggcggc tgcattgata ttagtcaacg aagacattca
     3061 agcatggacg gcgcgccggc caaaggctgt tcgcgaaccg gtcgagagtg ctacccgact
     3121 gcttcgccgc aaagtagcat tgccgcgaat cgcgcaatcc gggggagcgg gcgatggcgg
     3181 aaatcatagg ccttgtgctt cttatccttg cgtttccggt catctccgtc attgccctgg
     3241 tgaaggcgct gaatacccgc gacagcgtcc ggcggctgga acttcgtatc gcggtgctgg
     3301 aggccggtcc tgcccgccgg ccaggcgagg ccacggcccc cgagcctgtg cccccggtag
     3361 cggttgagca accaacgatc gcggagccgg ttgccgcccc ggaaacgcct gagccggctg
     3421 cggcgccggt agcgccggcg ccggcatcga ccacgttgga agagcgtttc ggcacacaat
     3481 gggtcgtctg gatcg
//
LOCUS       DASSRL010000409         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1008284, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000409 DASSRL010000000
VERSION     DASSRL010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008284"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFL49_08325"
     CDS             complement(<1..401)
                     /locus_tag="VFL49_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7912022.1"
                     /translation="MATIDRQQAFSGTKDVAASLRIDVASLERYLAANVAGFAGPLTV
                     RQFKGGQSNPTYLLETPRRRYVLRRKPPGKLLPSAHAVDREYRVIRALHAQGFPVAQP
                     LAYCADESVAGTPFYVMDFVDGRVFWEPQMPG"
     gene            complement(415..1077)
                     /locus_tag="VFL49_08330"
     CDS             complement(415..1077)
                     /locus_tag="VFL49_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912023.1"
                     /translation="MHPMMRLYEDVLSSAENVEFRLPPLPRFIFIVHGSARIAGATLD
                     EGEAWQGESAITVKPGSAGVTCWRWELARGDQGSTTANAPGMTTHEKLTAFLETMPKG
                     ELLMRGDSVAFPPGGCAYQHRHQGPGIRCLIEGGIRIDTHGRSTSFGPGGAWYESGPD
                     AVFAQASSEKSSRFIRVMILPRELVGKSSIQYVNEEDKAKPKSQQYQIYADAPISFGA
                     RK"
     gene            complement(1093..1995)
                     /locus_tag="VFL49_08335"
     CDS             complement(1093..1995)
                     /locus_tag="VFL49_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7912024.1"
                     /translation="MPDLADLFPNFVSQWIDTSIGKIFARIGGSGTPLLLLHGYTQTN
                     IMWHRVAPLLAERWSLVIPDLPGYGWSDVPRADEKHAPYDKRSMAKVMIEAMEKLGHA
                     RFRLAGHDRGGRVAYRLALDHPGRVVRMVTLDIVPTYDMWHRMDRNMAMKVWHWPFLA
                     QPEPLPEMLITKSPIDYLEWKMASWTKAKDLSAFDPRALDHYRAAFSDPLRIHAHCED
                     YRAGRWADLANDEADRKASKTIDCPLFALWGGAGIASESGGPLEIWKRWAPGAAGRPI
                     DSGHFLTEENPDATAAAMVEFLAA"
BASE COUNT          383 a          665 c          684 g          398 t
ORIGIN      
        1 ccgggcattt gcggctccca gaacacgcgg ccatcgacaa agtccatgac gtaaaacggc
       61 gttccagcca cgctctcgtc ggcacaatag gcaagcggct gtgcgaccgg aaaaccttgc
      121 gcgtgcagcg cgcggatgac ccggtattcg cggtcgaccg catgggcgga gggaagcaat
      181 ttgccgggcg gcttccgccg caggacgtaa cggcgtcgcg gtgtttcgag caggtaggtc
      241 gggttcgatt gtccaccctt gaattgccgg accgtgagcg gtcccgcgaa gcctgctaca
      301 tttgcggcga gataacgttc aaggcttgca acatcgatcc gcagtgaggc cgccacgtcc
      361 ttggtgccgg aaaaggcctg ctggcggtcg atcgtcgcca ttgccgttag accgttactt
      421 cctcgccccg aacgagatcg gtgcgtcggc gtagatctgg tactgctggg acttcggctt
      481 cgccttgtcc tcctcgttga cgtactggat cgagcttttg ccgacgagct cgcgcggcag
      541 gatcatcacg cggatgaaac ggctggactt ttcagacgac gcctgggcga acacggcgtc
      601 tggcccggat tcataccagg cgccgccggg cccgaacgag gtcgagcggc cgtgcgtgtc
      661 gatgcggatg ccgccctcga tcaggcagcg aattcccggc ccctgatgac ggtgctgata
      721 ggcgcagccg ccgggcggaa aggcgacgct gtcgccgcgc atcagcaatt cgcccttcgg
      781 cattgtttcg agaaaggcgg tgagcttttc atgcgtggtc atgccgggcg cgttggcggt
      841 ggtgctgccc tgatcgccgc gcgcgagctc ccagcgccag caggtgacgc ctgcggagcc
      901 cggcttcacc gtgatggcac tttcaccctg ccaggcttcg ccttcatcca gagtcgcgcc
      961 ggcgatcctg gcggagccat gcacgataaa aatgaaccgc ggcaacggcg gcagccggaa
     1021 ctcgacgttc tcggcactgg acagcacgtc ttcatacagc cgcatcattg gatgcattca
     1081 tagctcctga aatcacgccg caaggaattc gaccatcgcc gcggccgtcg cgtcgggatt
     1141 ttcctcggtg aggaagtgac cggagtcaat cggccggccg gctgcccccg gtgcccatcg
     1201 tttccagatt tcgagcggcc cgccggactc gctggcaatg ccggctccgc cccagagggc
     1261 gaaaagcggg cagtcgattg tcttgctggc cttgcggtcc gcttcgtcat tggcaagatc
     1321 ggcccagcgc ccggcgcgat agtcctcgca gtgcgcgtga atgcgcagcg ggtccgaaaa
     1381 ggcggcgcgg taatgatcca gcgcgcgcgg gtcgaaagcc gacaggtctt tcgccttggt
     1441 ccaactcgcc atcttccatt caagatagtc gatcggcgac ttcgtgatca gcatttccgg
     1501 caacggttcc ggctgcgcca ggaaaggcca atgccagacc ttcatcgcca tgttgcgatc
     1561 catccggtgc cacatgtcgt aggtcggcac gatatcgagc gtcaccatgc gcacgacgcg
     1621 tcctggatga tcgagcgcga gccggtaggc aacgcggccg ccgcggtcgt gtccggcgag
     1681 ccggaagcgc gcatggccga gtttttccat tgcctcgatc atcaccttcg ccattgagcg
     1741 tttgtcgtaa ggcgcgtgct tctcgtcggc acgcgggacg tccgaccagc cgtagcccgg
     1801 tagatcgggg atcaccagag accagcgctc cgcaagcagc ggtgccacgc ggtgccacat
     1861 gatattggtc tgcgtatagc cgtgcaaaag cagaagcggc gtcccgctgc cgccgatgcg
     1921 cgcgaagatt ttcccgatcg atgtgtcgat ccattgcgag acaaaattgg gaaaaagatc
     1981 ggcgaggtcg ggcatcgtag tccgcaaatg acgtcgaagg gcgattgcaa ttttcgtcgt
     2041 cccgggcgag cgatagcgag gcccgggacc catacgccac agtctatcaa taggctgcgg
     2101 cgtatgggtc cccgctttcg cggggacgac
//
LOCUS       DASSRL010000410         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1462927, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000410 DASSRL010000000
VERSION     DASSRL010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462927"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFL49_08340"
     CDS             <1..404
                     /locus_tag="VFL49_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7912025.1"
                     /translation="GVLLGSRLLMTEEALVPSGFHDAIIRADGDATIKTRVIDVVRGL
                     NWPTEITGRALRNRFVADWQGHEATLAEPATTARELLRYTRARDGGDTDNTGIFIGEV
                     AGLLKDVRPADTIVKALVAEAEDLIAGRRPQ"
     gene            401..1282
                     /locus_tag="VFL49_08345"
     CDS             401..1282
                     /locus_tag="VFL49_08345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7912026.1"
                     /translation="MNKSPVAIVGLGLMGEVYARRLLDADAAVIGFDVDPARRARLAE
                     IGGRPVQSIAELAKPARCIIIAVFSTDQVEDVVENQLLPALGDGSGKIVLCMSTCDPD
                     QVAALAKRVTPRGIRYLDVPVSGTSDQVRRGDGVALIGGDVAIADEVKDVLDALFARR
                     FHVGDVGDGGRAKLAVNLILGLNRVALAEGLVFAERLGLDPVAFLKVARGSASYSQVM
                     ETKGAKMVEGDFSPEGRVRQTLKDARLMLDQAAAIGQKLSMLEVHADILEACVRAGEA
                     EFDNSAVIREVRRRGRN"
     gene            complement(1279..>2196)
                     /locus_tag="VFL49_08350"
     CDS             complement(1279..>2196)
                     /locus_tag="VFL49_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7912027.1"
                     /translation="QGKSKHLRFIRGVTKEPELVRDFAAAKEFELSTEFEEAIADPRV
                     RAVFLATPHSLHVRQISAVAAAGKPVWCEKPLALTRADAERAVEATKKAGVPFALGNN
                     KRCFPCMRELERLVDAGALGEILHIEGNFSNEHSTRVTGGWRDDPHESPGGGMTGAGL
                     HVLDAFVNLAGPIAKVDARMHVKKPPPDPRDVAAAMMQFESGATGLLATVRAAPMFWR
                     IHVFGTKGSAEAREETSLTVAMIGSQPETKSFPPADSLGVLLEAFAETIETGKLFPVS
                     TTDMLDVVSAFEATIRSIAEGKPVAVTRG"
BASE COUNT          386 a          634 c          729 g          447 t
ORIGIN      
        1 atggcgtgct gctcggttcg cgcttgctta tgactgagga ggcgcttgtt ccttccggtt
       61 ttcacgatgc cataatccgc gccgacggtg acgcgactat caagacaagg gtgatcgacg
      121 tcgtgcgcgg ccttaactgg ccgaccgaaa tcacggggcg agccttgcgt aaccgattcg
      181 tcgccgattg gcaggggcac gaggcgacac ttgccgagcc cgccacgacg gcgcgggagt
      241 tgcttcgcta cacgcgggcg cgggatggcg gtgacaccga caatacgggc attttcatcg
      301 gcgaggttgc gggcctgctt aaggatgttc ggccggccga cacgatcgtc aaggctctag
      361 ttgctgaagc cgaggacctg atcgcgggac ggcgcccgca atgaacaagt ccccggtcgc
      421 gatcgtcggt ctcggtttga tgggcgaggt ctatgcccgc cgtcttcttg atgcggatgc
      481 tgcggtgatc ggtttcgacg tagatccggc ccgccgcgcg cggctggcgg agatcggagg
      541 ccggcccgtt caatcgattg ccgaactggc aaaacctgca cgctgcatca tcatcgcggt
      601 gttcagcacg gaccaggtcg aagacgtggt tgagaaccag ctgttaccag cgctcggtga
      661 cggctcaggc aagatcgtcc tgtgcatgag cacatgtgat cctgaccagg tggcggcgct
      721 tgcgaagcgc gttactccgc gcggcattcg ctatctggat gtcccggttt ccggcaccag
      781 cgatcaggtg cgtcgcggcg atggcgtcgc ccttatcggc ggcgacgtcg cgatcgctga
      841 cgaggtgaaa gacgttctgg acgcactttt cgcgcgacgg tttcatgtcg gcgatgtcgg
      901 tgatggcggc cgcgccaagc tcgccgtcaa tttgattctg ggactcaatc gggtggcgct
      961 tgcggagggc ctcgtttttg ccgaacgcct cgggcttgat ccagtggcgt tcctgaaggt
     1021 ggcgcgcggc tcggcttcgt attcccaggt tatggaaacc aaaggtgcga agatggtcga
     1081 gggtgatttt tcgcctgaag gccgtgtcag gcagactttg aaggatgcgc gcctgatgct
     1141 cgaccaagcg gcggcaatcg ggcaaaaact gtcaatgctc gaagtgcacg cagacatact
     1201 tgaagcctgt gtgcgtgccg gtgaagccga gttcgacaat agtgcggtta ttcgggaggt
     1261 gcgccgacgc ggcagaaatt agccgcgtgt gaccgcaacc ggtttgccct cggcaattga
     1321 gcgaattgtc gcttcgaaag cgctgacaac gtcaagcatg tcggtcgttg acacagggaa
     1381 gagttttccc gtttcgatcg tctcggcgaa ggcctcaagc agaacgccaa gcgagtccgc
     1441 aggcggaaag cttttcgttt cgggttggct gccgatcatc gcgactgtga gtgaagtttc
     1501 ctcgcgtgcc tccgcggagc ccttggtgcc gaagacgtga attcgccaga acatcggtgc
     1561 agcgcgcacg gtggcgagca ggccggtcgc gccgctttcg aattgcatca ttgccgccgc
     1621 cacatcgcgc ggatcgggag gcggtttctt gacatgcatc cgcgcatcca ctttcgcgat
     1681 aggcccggcg agattcacga atgcgtcgag cacgtgcaag ccggcgccgg tcatgccgcc
     1741 gccgggcgat tcatgcgggt cgtcgcgcca tccgcccgtg acgcgggttg aatgctcgtt
     1801 gctgaaattg ccttcgatat gcaggatttc gcccagagcg ccggcatcta ccagacgttc
     1861 gagctcgcgc atacacggaa agcagcgctt gttgttgccg agcgcgaagg gcacgccggc
     1921 cttttttgtg gcctcgacgg cgcgttcagc gtcggcgcgc gtgagcgcca gcggcttctc
     1981 gcaccagacc ggcttgcccg ccgcagcgac cgcagagatc tgtcgcacgt gaagggaatg
     2041 cggagttgcg agaaagacag ctcgcacgcg ggggtcggcg atcgcctctt cgaactcggt
     2101 cgatagttcg aactctttag cggccgcgaa atcacggacc agttcaggct ccttggtgac
     2161 accgcggatg aagcgcaggt gcttgctttt gccctg
//
LOCUS       DASSRL010000411         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_98474, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000411 DASSRL010000000
VERSION     DASSRL010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98474"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(587..1834)
                     /locus_tag="VFL49_08355"
     CDS             complement(587..1834)
                     /locus_tag="VFL49_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HET7912028.1"
                     /translation="MLELVATALFALAVLHTFMTRYFEYLARLQPNHAGLWHLLGEVE
                     TVFGIWAVALLAFMAAYASPQAAQSYLESRTFTEPAFIFAIMVIAASRPILDFSGVML
                     HWMARLLPLQRPIAYYFTVLFIGPLLGSFITEPAAMTLSALLLRERYFSRQAPRAFKY
                     GTLGVLFVNVSIGGTLTPFAAPPVLMVAERWGWDFGFMLQTFGWKAALAVLVNAAAAT
                     FAFRSYLAQLPTISTALARLDVPWPIVGAHLLFLVGIVVAKHHLVVFAVIFLLFLGFA
                     EAYRRYQSRLILREGLMVGLFLGGLVVLGGLQGWWLSPLLSNMSSTALYYGATALTAV
                     TDNAALTYLGSLVDGLPADAKYSLVAGAVSGGGLTVIANAPNPAGFAILKNTFDDGSI
                     NPGWLALAAAPPTVTAVAAFQLL"
     gene            complement(1858..2247)
                     /locus_tag="VFL49_08360"
     CDS             complement(1858..2247)
                     /locus_tag="VFL49_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912029.1"
                     /translation="MINKRRARPNHEQGDIVILCAEDELRDAIAYWLSSQPAKTVIAD
                     DGYHAARLLQNGCQWLVTDRVLPPWPGLDNFLNLRATHPQLRIAFIENGNLDDRILAR
                     ATGASVLLPRPLSRKTVSDALTSTSDR"
     gene            2395..>3433
                     /locus_tag="VFL49_08365"
     CDS             2395..>3433
                     /locus_tag="VFL49_08365"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET7912030.1"
                     /translation="MAGSKLERRSLPMAFPRVFSLSPPVIGIGYLAIYVLLDWVSFIQ
                     PFAPFGITPWNPSTGSSFIVVLLFGQRYLPLLFAAPLLADLLVRQLPLPWPVELAACA
                     VIGGGYAAGLSILLRPATRFNPALASMRDLILLLGVAAASSAAVAVCYVGVLVAAGLL
                     TPQVYLPAALQFWIGDVIGVAVVAPFALIVLTRGRVLSMTRETVLQIVAILAALVFVF
                     VYAEKHRFEFFYILFLPVIWMAIRGGLELVTIGILLTQVGLILSVQLLPREGVDVAAF
                     QALMLVLALSGLAAGAVVTEHRRTEAQLRLHQNSLAHVARLGTIGEFAAMIAHEINHP
                     LMAAGTYTRLTA"
BASE COUNT          682 a         1015 c         1047 g          689 t
ORIGIN      
        1 ggtcgtcagg tggcgtgcgt atgacctgaa cgtgggagca actagagcag cgccaatagg
       61 ttgtgaccgc accgtcgtca tggacaatcc gattgagcgt ttccatttca ccgcgtgcgc
      121 aaaccacaca cagtttcttc gccggtagct ggctcgtttg cttcaccccg taaatttcgc
      181 ataaactttt ttcggggcgc aagttatggg cgtagcgatt ctaacaataa tttttctcgt
      241 tctagacgga ctactatggt accagcgccc aaatcctcat ctttggcttt ttggcaccct
      301 tttccttggg gcggcttggg gaatggggtt cttgtggctg gtcacgcgta agcgataacc
      361 agccctcttg gctggcagct gctaaagcgt tgcggcgttg gcgatcacaa tcgctgcgcg
      421 actggcgtcg ccgtcacttt ttcgtcggca tggcgcgcct ttcgaagaac aaggacgcca
      481 ggccggccaa tgcaaccagg aacggaaggg ccagcaagaa cgcgaaggga gcgtcaagag
      541 aggttagcca ctcccataat tcagggagcc agtgcgtcat ggcgcctcac aacaactgga
      601 aggccgcaac agcggtgacg gtgggaggcg ctgcggcgag cgcgagccag ccgggattga
      661 tcgaaccgtc gtcaaacgta tttttgagga ttgcaaaacc cgccgggttt ggtgcgttcg
      721 cgatgacggt aagcccgccg ccgctcaccg cacccgccac cagcgaatat ttggcgtcag
      781 ccggcagtcc atcgaccagc gacccaagat aagtgagcgc cgcgttgtcc gtcaccgcgg
      841 tcagcgcggt ggcgccatag taaagcgcgg tgctgctcat gttggagagc agtgggctca
      901 gccaccagcc ctgcaggccg ccgagaacga ccagacctcc caggaacagc cccaccataa
      961 ggccttcgcg caggatcaga cggctttgat agcggcgata ggcctccgca aaacccagga
     1021 aaagcagaaa aatcacggcg aaaacgacga gatgatgctt ggcaaccacg atcccgacca
     1081 ggaacaggag atgtgctccg acaatcggcc aaggcacgtc gagccgcgcc agcgcggtgg
     1141 agatcgtcgg caactgtgcg aggtaagacc gaaaagcaaa agttgctgca gctgcattga
     1201 cgagtacggc gagagcagcc ttccaaccga aggtctgcaa catgaagccg aagtcccagc
     1261 cccagcgctc cgcaaccata agtacgggcg gagccgcgaa cggcgtcagt gttcctccga
     1321 tcgacacatt gacgaaaagc acgccaagcg tgccgtactt gaatgcgcgc ggcgcctgtc
     1381 ggctgaaata acgctcacgg agcagcaatg ccgaaagcgt catcgcggcc ggctcggtga
     1441 taaatgaacc gagcaagggg ccgatgaaca gcaccgtgaa ataatacgca atcggacgct
     1501 gcagcggcag aagccgcgcc atccagtgca gcatgacccc ggaaaaatcc agaatcggac
     1561 ggctcgccgc gatcaccatg atcgcaaaga tgaaagccgg ttcggtgaac gttcggctct
     1621 caagatagct ctgcgcagcc tgcggggaag cgtaggctgc catgaaagcc agcagcgcga
     1681 ctgcccagat accaaacacc gtctccactt cgccaagaag gtgccagagg cctgcatggt
     1741 tcggctgcag gcgcgcgaga tattcaaaat agcgcgtcat gaaggtgtgc aagaccgcaa
     1801 gggcaaacag cgcggtcgcc accagctcaa gcatgcttgg atgaacgccg cctaggttca
     1861 tctgtcggac gtcgatgtca gggcgtcgct taccgtcttg cggctcagtg gccgcggcag
     1921 caacacgctc gcgccggttg cgcgtgcgag gatgcggtcg tcgagattgc cgttctcaat
     1981 aaatgcgatg cgaagctggg gatgcgtggc ccgcaagttc aggaaattgt cgagtccggg
     2041 ccacggcggc agcacacgat cggtgacgag ccactgacag ccgttctgca agagtctcgc
     2101 ggcatggtag ccgtcgtctg caatgaccgt ctttgcaggt tgcgacgaaa gccagtaggc
     2161 aatagcgtcg cgaagttcgt cctccgcgca caggataacg atatcgccct gctcatgatt
     2221 ggggcgagcg cgtcgtttgt tgatcaatct gccgggcgca ttcatacgcg cgaaggtggg
     2281 cgtgaataca gcgctttgac aattcagggt gcgcgcctag gctcgaatac ctcggtacaa
     2341 ccacctgggt acgcacgcgt agcagccgcg cgcgcgcagc agcgataaag agacatggcc
     2401 ggttcgaaac tcgaacgtcg atcactgccg atggctttcc cgcgcgtctt ctcgttgtcg
     2461 ccgcccgtca ttggcatcgg ctaccttgca atctacgtgc ttttggactg ggtcagcttc
     2521 atccagcctt tcgcgccttt cggcatcaca ccatggaatc cttcgaccgg atcaagcttc
     2581 atcgtggtgc tgttattcgg gcaacggtac ttgccgctgt tgttcgcggc gccgttgttg
     2641 gcggatcttc tggtacggca gctaccgctg ccatggccgg tcgagcttgc ggcctgcgcc
     2701 gttatcggcg gcggctatgc ggccgggctc agcattctgt tgcggccggc gacgcgcttc
     2761 aatccggcgc tcgcctcgat gcgcgatctt atcctgctgc tcggggtggc tgccgccagc
     2821 agtgctgctg tggcggtctg ctatgtcggt gtcctggttg ctgccggcct gctcactccg
     2881 caagtttacc ttcccgccgc gctgcagttc tggatcggag atgtcattgg cgtcgccgtg
     2941 gtcgcccctt tcgccctgat cgtgctcacg cgtgggcgtg tcctgtcgat gacaagagaa
     3001 accgtgctgc agatcgtcgc tattctggcc gcgcttgtgt ttgtgttcgt gtatgccgag
     3061 aagcaccgct tcgagttttt ttatattctg ttcctgccgg tgatctggat ggcgattcgt
     3121 ggcggtctcg agctggtgac catcggcatt ctgctgacac aggtcggtct catcctcagc
     3181 gttcagctgt tgcctcgcga aggcgtcgat gttgccgcgt ttcaggcgct gatgctggtg
     3241 ctcgccttga gcgggctcgc cgccggcgct gtcgttaccg aacaccggcg caccgaggcg
     3301 caacttcgtc tgcatcagaa ctctctggct cacgtcgcgc ggcttggcac cattggcgag
     3361 ttcgcggcca tgatcgcgca tgagatcaat catccgttga tggcagcggg tacctacacg
     3421 cggcttacag ccg
//
LOCUS       DASSRL010000412         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_273045, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000412 DASSRL010000000
VERSION     DASSRL010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273045"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /gene="rpsC"
                     /locus_tag="VFL49_08370"
     CDS             complement(<1..643)
                     /gene="rpsC"
                     /locus_tag="VFL49_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7912031.1"
                     /translation="MGRKVNPIGFRLGIVSDWESKWFAERNYTEQLQEDIKIRELIMK
                     ELQRAGISKVELERSANKVDVTLHTSKPGIVIGKQGSNVERIRQLLEKEVGKKVHLKI
                     EEIKVPEIDAKLIAESIAEQIGRRVAYRRAMKHAVQQAMRRGAKGVKIRLGGRLGGAE
                     MSRTVTEMDGRVPLHTLRADIDYGVVHAHTTYGRIGVKVWVYKGEVMPTRGEVT"
     gene            complement(644..985)
                     /gene="rplV"
                     /locus_tag="VFL49_08375"
     CDS             complement(644..985)
                     /gene="rplV"
                     /locus_tag="VFL49_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7912032.1"
                     /translation="MEVRAHLGRVRVSARKARLVVDVVRGRPVLEAQAMLRFIPNKTA
                     FELEKLMKSLAANAENNYDLDPRDLVIKEIYADEGPTMRRFRAKARGRVGRINKRTCS
                     ITVVAEDRGGN"
     gene            complement(1000..1281)
                     /gene="rpsS"
                     /locus_tag="VFL49_08380"
     CDS             complement(1000..1281)
                     /gene="rpsS"
                     /locus_tag="VFL49_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006823225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7912033.1"
                     /translation="MGRSSKKGPYIDAKLQAKVETLNRGNDRKVIRTWARDCTIFPGM
                     VGHTIAVHNGRQHVPIFVTEPMVGHKLGEFAPTRTFRGHGKDADRRGRR"
     gene            complement(1329..2168)
                     /gene="rplB"
                     /locus_tag="VFL49_08385"
     CDS             complement(1329..2168)
                     /gene="rplB"
                     /locus_tag="VFL49_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019297986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7912034.1"
                     /translation="MPIRKYKPTSPGRRSMSVSTFEEITKSRPEKSLIVPLKKQSGRN
                     HHGRITTRHRGGGHKRFYRIIDFKRNKFGIPARVAAIEYDPNRSARIALLFYADGEKR
                     YMLAPLGLNVGDTVVSGPEAPIRNGNALPLRNIPTGSQIHNVELRKGKGGQLVRSAGV
                     SAQLMAKSDDYAQIRMPSGEVRRVHIDCMATLGQVGNVDHENIEIGKAGRSRYLGRRP
                     TVRGSVMNPRDHPHGGGEGKAPIGGQPKTKWGKPAFQRTRNNKRTDSMIVRRRPANRG
                     RSR"
     gene            complement(2203..2514)
                     /gene="rplW"
                     /locus_tag="VFL49_08390"
     CDS             complement(2203..2514)
                     /gene="rplW"
                     /locus_tag="VFL49_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7912035.1"
                     /translation="MADLRLEDVIKRPLITEKNTILMEQGQYSFEVAPAANKFQIKAA
                     VEQTFKVTVRAVNTLNVKPRKKSRTASRRGGRIEGTQPGWKKAIVTLAPGERIDVFEQ
                     V"
     gene            complement(2507..>2713)
                     /locus_tag="VFL49_08395"
     CDS             complement(2507..>2713)
                     /locus_tag="VFL49_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912036.1"
                     /translation="GNLENVHWVLASYLNVRDILKYERLVVMKSSIEVIEKIWALPAD
                     KREPSVWSQARRAVRAQAQEANRG"
BASE COUNT          432 a          913 c          781 g          587 t
ORIGIN      
        1 cggtgacttc gccacgcgtc ggcatgactt cgcccttgta aacccagacc ttgacgccga
       61 tgcggccgta cgtggtgtga gcatggacca cgccataatc gatatccgcc cgcagggtat
      121 gcagcggcac ccggccatcc atctccgtga cggtccggct catctcggcg ccaccaaggc
      181 ggccacccag ccgaatcttc acgcccttgg cgccacgccg cattgcctgc tgaaccgcat
      241 gcttcatcgc ccgccggtac gcgacgcggc gaccaatctg ctcggcgatg ctttccgcga
      301 tcagcttggc gtcgatctcg ggcaccttga tctcctcgat cttgaggtgc accttcttgc
      361 cgacttcctt ctcgaggagc tggcggatgc gctcgacgtt cgagccctgc ttgccaatga
      421 cgatgccggg cttcgaggtg tgcagcgtga catcgacctt attggccgag cgctcgagct
      481 cgaccttgga aatgccggcc cgctgcagct ccttcatgat cagctcccgg attttgatgt
      541 cctcctggag ctgttcggtg tagttgcgct ccgcgaacca cttggattcc cagtcgctga
      601 cgatcccgag cctgaacccg atcgggttta ctttgcgtcc catctagttt cctccccgat
      661 cctctgcgac gaccgtgatg ctgcaggtgc gcttgttgat gcgccccacg cgaccacgcg
      721 ccttggcccg gaatcgccgc atcgtcgggc cttcgtccgc gtaaatctcc ttgatcacga
      781 gatcgcgcgg atccaggtcg tagttgttct ccgcgttcgc cgcgagcgac ttcatcagct
      841 tctcgagctc gaacgcggtc ttgtttggaa tgaagcggag catggcctga gcttcgagga
      901 ccggtcgtcc ccgcacaaca tcgaccacga gccgtgcctt gcgtgccgaa acccggactc
      961 gccccaggtg agcgcgtact tccatctctc ccctcaccct taccggcgac cgcggcggtc
     1021 ggcatccttg ccatgaccgc ggaaggtacg tgtcggcgca aactcgccca gcttgtgacc
     1081 gaccatcggc tcagtcacga aaatcggcac atgctgccgg ccgttgtgga cagcgatcgt
     1141 gtgccccacc atgcccggga agatcgtgca atcgcgcgcc caggtgcgga tgaccttgcg
     1201 atcattgccc cggttgagcg tctcgacctt cgcctgcagc ttggcgtcga tatatggtcc
     1261 ctttttcgat gatctaccca taatgatcct ccgcgacgcc gccggttgat taccggcgag
     1321 cggtcaccct atctcgaccg cccccgattc gccggacggc gacgaacgat catggaatcc
     1381 gtgcgcttgt tgttgcgcgt ccgctggaat gctggcttgc cccacttggt cttgggctgg
     1441 ccaccgatcg gcgccttgcc ctcgccaccg ccgtgcggat ggtcgcgcgg gttcatcact
     1501 gagccacgga ctgtcgggcg gcggccaagg tagcggctgc gtccggcctt gccgatctcg
     1561 atattttcgt gatcgacatt gcctacctgg ccgagcgtcg ccatgcagtc gatatgcacc
     1621 cgccggactt cgccggacgg catccggatc tgggcatagt cgtcactctt ggccatgagc
     1681 tgtgccgaca cgccggccga gcgaaccaat tggccgcctt tgcccttgcg caactcgacg
     1741 ttgtgaatct gcgagccggt cggaatgttg cgcagcggca aggcattgcc attgcggatc
     1801 ggtgcttccg gtcctgaaac gaccgtgtcg ccaacgttca gccccaacgg cgccagcatg
     1861 taccgcttct cgccatccgc gtagaacagc agcgcaatgc gggcggagcg gttcgggtcg
     1921 tactcgattg cggcaacccg cgccgggatt ccgaacttgt tccgcttgaa atcgatgatg
     1981 cgatagaacc gcttgtgtcc gccgccgcga tggcgggtcg tgatgcggcc atgatgattg
     2041 cgacccgact gcttcttcag cgggacaatc aggctcttct ccgggcgcga cttcgtgatc
     2101 tcctcgaagg tgctcacact catcgagcgg cgtcccggtg atgtcggttt gtatttgcga
     2161 attggcatcg cctggctccg attccttccg tactcgagcg tgctagacct gctcgaagac
     2221 atcgatccgc tcacccgggg cgagcgtcac gatggctttc ttccagccgg gttgcgttcc
     2281 ttcgatccga ccgccgcgac ggctggccgt ccgcgacttc ttgcgcggct tgacattcag
     2341 cgtgttgacc gccctcaccg tgaccttgaa ggtctgctca acggcggcct tgatctgaaa
     2401 cttgttggcg gcaggcgcga cttcaaacga gtattgcccc tgctccatca aaatggtgtt
     2461 cttctcagta atcagcggac gcttgatcac gtcctccaag cgaagatcag ccacggttgg
     2521 cctcctgtgc ctgcgcacgg acggcccgcc gcgcctggga ccacacgctc ggctcccgct
     2581 tgtcggctgg caacgcccaa atcttctcga tcacttcgat cgacgacttc atgacgacca
     2641 ggcgctcgta cttcaggatg tcgcgcacat tgaggtagct cgcgagcacc cagtgcacgt
     2701 tctcgaggtt gcc
//
LOCUS       DASSRL010000413         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_413557, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000413 DASSRL010000000
VERSION     DASSRL010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413557"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..1062)
                     /locus_tag="VFL49_08400"
     CDS             complement(364..1062)
                     /locus_tag="VFL49_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7912037.1"
                     /translation="MATIRIMSGGAPKEIFLQLTSQFERESGHKVDYVFAVMSALRDK
                     LAAGEKADVLVMPANILDGYQKDNVVRAQGRAVLGLVSINAVVRAGAPKPDLSTPEKV
                     KQTMLDSRAITHATPGATPSGTHMGKLVGQLGIADAMKGKIIHRPALEGGVQLVASGE
                     AEIGFYPKSEVVNTDGLTVVGPLPAAIQLTTIYGAAVTAASPAPDAAAAFIKFMTDPA
                     HGAAWAHAGFDPPA"
     gene            complement(1072..2058)
                     /locus_tag="VFL49_08405"
     CDS             complement(1072..2058)
                     /locus_tag="VFL49_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912038.1"
                     /translation="MAKLNLSVAVGPYDRTRALIDGTVQIDGVKPACMTLSPEEIFFR
                     AFRQAEFDICELSLSSFTVKTAQGNCPYVGVPAFVSRTFRHTSIYVRTDRVKKPADLK
                     GKKVGLPEYQLTANVWARAILQDDHGVKPSDIHWVRGGIEDADRPEKISIKLPPDVKL
                     EDGPKGKSISALLRDGEIDGFIAPRPPSLPKDTPNVGWLFPDPVAAAKDYFKRTGIFP
                     IMHLVGVRRTLAEKHPWLPGAVFKAFEQAKKVALEQLSDTSATKVTLPFVEERLSEAH
                     ALMGEDFWSYGVAANRKTLETFLHHHHSQGLSSRLVTVEEMFHPGTLETFKL"
     gene            complement(2118..2795)
                     /locus_tag="VFL49_08410"
     CDS             complement(2118..2795)
                     /locus_tag="VFL49_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7912039.1"
                     /translation="MSRLVRVIFAGALLPGLASYAVAQAAKETPRTYGIGHVATAAQI
                     AGWDIDVRPDGQGAPPGRGSVKDGEKVYMEKCAACHGEFGESAGRWPQIAQGKGTLAT
                     HDPVKTVGSYYPYLSSAFDYIRRAMPFGDAESLTNDELYAVVAYVLNLNDIVDDNFVL
                     SKESWAQVKMPNAGGFFDDDRETSEKAFWNPDPCMKDCRGPVAITGHAQAIDVTPDEK
                     TQKRGVE"
     gene            complement(2779..>3133)
                     /locus_tag="VFL49_08415"
     CDS             complement(2779..>3133)
                     /locus_tag="VFL49_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HET7912040.1"
                     /translation="QAPLKHKSGFTVLSGIAWSGRGKIARVDVSLDGGRNWRAARIDG
                     PVWDKALTRFYYEFDWDGRELLLQSRAMDETGYVQPTKAELRKLRGVNSIYHNNGIQT
                     WALKADGEVENVEVG"
BASE COUNT          560 a         1019 c          934 g          620 t
ORIGIN      
        1 ccgtacccga cgttgtcggc gagaatgaac acaacattcg gcttctgtgg ctgctgtgcg
       61 atggcttgcg tggccgcgat aaaaaagacc gatgcgagaa gaagtctgtt cactggctgt
      121 ctccacgact gaccgttcga gcgcaaaatt ctcaccgcaa ggtagatggt ttttctccag
      181 gtactgtccc cttttagctg tcggcgctct agtgcccttt agggcaatcg cttgtcgtag
      241 taatatccgc tgtgcgccaa tcgcgacatt tgcctgcttt tgggatgtcc gctctgcccc
      301 gatagcgaca atgctcgcca ccgctgacta tggccggccg ttcatgagag tccgcatcct
      361 agactaggcc ggcggatcaa agcctgcgtg tgcccacgca gcaccgtgcg cgggatcggt
      421 catgaatttg ataaatgccg ccgcggcgtc tggcgccggg ctcgccgccg tcaccgccgc
      481 gccatagatg gtggtgagtt ggatcgcggc cggcagcggg ccgacgacgg tcaggccgtc
      541 ggtgttgacc acctcgctct tcggataaaa gccgatctcg gcctccccgc ttgcaacaag
      601 ctgcacgccg ccttcaagcg ccgggcggtg gatgatcttg cctttcatcg cgtcggcgat
      661 gccgagctgc ccgacgagtt ttcccatatg cgtgccgctc ggtgtggcgc cgggcgtcgc
      721 gtgcgtgatg gcgcggctat cgagcattgt ctgcttgacc ttctcgggcg tcgacaggtc
      781 cggcttcggg gcgcccgcgc gcaccaccgc gttgatgctc accaggccaa gaaccgcgcg
      841 gccctgcgcg cgcacgacat tatccttctg atagccgtcg aggatgttgg ccggcatcac
      901 caggacatcg gccttctcgc cggccgcaag cttgtcgcgc agagccgaca tcaccgcgaa
      961 gacatagtcg accttatgac cggactcgcg ctcgaactgc gacgtcaatt gcagaaaaat
     1021 ctccttcggc gcgccgccgc tcatgatgcg aattgtcgcc attgcaatgt cctaaagctt
     1081 gaaggtctcg agcgtgccgg ggtggaacat ctcttccacc gtcaccaggc gcgacgacaa
     1141 cccttgcgaa tgatggtgat gcaggaaggt ctccagcgtc ttgcggttgg cggcaacgcc
     1201 gtaggaccag aagtcctcgc ccatcagcgc gtgcgcttcc gaaagcctct cctcgacgaa
     1261 cggcagcgtc accttcgtcg ccgaggtgtc gctcagttgc tcgagcgcca ctttcttggc
     1321 ctgctcgaag gccttgaaca ccgcccccgg cagccaggga tgcttttcgg ccaaagtgcg
     1381 ccgcacgccc accaggtgca tgatcgggaa aatgcccgtg cgcttgaaat aatccttggc
     1441 ggcggcaacc gggtcgggaa acagccagcc aacattgggc gtatccttcg gcagcgacgg
     1501 cggacgcggc gcaatgaagc catcgatctc gccgtctctc agcagcgcgg aaatcgattt
     1561 tcctttcggc ccatcctcca gtttcacgtc aggcggtagc ttgatgctga tcttctccgg
     1621 acgatcggca tcctcgatgc cgccgcgtac ccaatgaata tcggacggct tcacaccgtg
     1681 atcatcctga aggatcgcgc gcgcccagac attggcggtg agctggtatt ccggcaggcc
     1741 gactttttta cccttcaggt cggccggctt cttgacgcgg tcggtgcgga catagatcga
     1801 cgtgtggcga aacgtgcgcg acacgaaggc cggcacaccg acatagggac agtttccctg
     1861 cgcggtcttc accgtgaaac tcgacagcga aagctcgcag atgtcgaatt ccgcttgccg
     1921 aaaggcgcgg aaaaaaattt cctccggtga aagcgtcata caagccggct tgacgccgtc
     1981 gatctgcacg gtgccgtcga tcagcgcccg cgtgcggtca tagggcccga cggcgacgga
     2041 aagattgagc ttggccatga aaaacccggg tggaaaacga aacgccgggc tgtcataggc
     2101 gaaatgacgt cgggacgcta ctccacccca cgcttttgcg tcttctcgtc cggtgtcacg
     2161 tcgatcgcct gggcgtgccc ggtgatcgca accggtccgc gacaatcttt catgcagggg
     2221 tcgggattcc agaaagcctt ctcactcgtt tcccggtcgt cgtcgaagaa gccaccggcg
     2281 ttcggcattt tgacctgcgc ccagctttct ttcgacagca cgaaattgtc gtccacgatg
     2341 tcgttgagat tgagcacgta ggcgaccacc gcataaagct cgtcgttggt gagcgactcc
     2401 gcatcgccga agggcatcgc acggcggata tagtcaaacg cgctagaaag atacggatag
     2461 tacgacccga ccgttttcac cgggtcatgc gtggcgagcg tgcctttgcc ctgcgcgatc
     2521 tgcggccagc gcccggcgct ctcgccgaac tcgccgtggc aggcggcaca tttctccatg
     2581 tagaccttct cgccatcctt caccgacccg cgcccgggcg gtgcgccctg cccatcgggc
     2641 cgcacatcga tgtcccaccc tgcgatctgc gcggcggtcg cgacatgtcc aattccatag
     2701 gtgcgcggcg tttccttggc cgcctgcgcc acggcgtagc tcgccaaacc cggcagcagc
     2761 gcgccggcaa agatgactct aaccaacctc gacattctcg acctccccgt cggccttgag
     2821 cgcccaggtc tggatgccgt tgttgtgata gatcgagttg acgccgcgca gcttgcgcag
     2881 ctcggccttg gtcggctgca catagccggt ctcgtccatc gcgcgcgatt gcaggagcag
     2941 ttcgcggccg tcccagtcga actcgtagta gaagcgggtc agcgccttgt cccagaccgg
     3001 cccgtcgatc ctggccgcgc gccagttgcg gccgccgtcg agcgagacat ccacccgcgc
     3061 gatcttgccg cggcccgacc aggcgatgcc ggacagcacc gtgaagccgg acttgtgctt
     3121 cagcggcgcc tgc
//
LOCUS       DASSRL010000414         4177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_588305, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000414 DASSRL010000000
VERSION     DASSRL010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588305"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VFL49_08420"
     CDS             <1..1175
                     /locus_tag="VFL49_08420"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7912041.1"
                     /translation="AAGVPEGTDVVDCRGKLVAPGLIDMRAFIGEPGEASRETLASAS
                     QAAAAGGVTTIICQPDTSPTIDDPATVDFVLRRARDTGIVHVHPMAAITKGLEGKEMT
                     EIGLLKAAGAVAFTDGARSITNAQVMRRVLTYAGDFDALIVHHVEDPNLVGDGVMNEG
                     EFAARLGLLGIPKTAETIMLERDTRLVALAGGRYHAAAVTCVETLEVLRRAKDAGLSV
                     TASASINHLTLNEIDIGPYRTFLKLSPPLRAEDDRKALVQAVADGVVDVVVSDHNPQD
                     VETKRLPFAEAAPGAIGLETMLTAGLRLVHNGEIELVPLLRAMSTRPAELLGLPGGTL
                     RSGAPADVIVVDTELPWVLDPNDLKSKCKNTPFDEARLTGRVVRTIVAGRTVYEYV"
     gene            1172..1765
                     /gene="plsY"
                     /locus_tag="VFL49_08425"
     CDS             1172..1765
                     /gene="plsY"
                     /locus_tag="VFL49_08425"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7912042.1"
                     /translation="MIAAAAFAFGYLLGSIPFGLLFTRAAGGPDIRAIGSGNIGATNV
                     LRTGHKALAAATLVCDMLKGAIAVLAVAHYGADAAIAAALGAFLGHLFPVWLKFKGGK
                     GVATFIGLLIGLYWPAALVFCIVWLAVAAISRYSSLAALVASAITPLAVWYFGLPRVA
                     ALFLLLTVLLWVMHRANIARLLNGSEGKIGAQGVGIN"
     gene            1822..2943
                     /gene="dprA"
                     /locus_tag="VFL49_08430"
     CDS             1822..2943
                     /gene="dprA"
                     /locus_tag="VFL49_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET7912043.1"
                     /translation="MGGTLQQRLSDAERLDWLRLIRTDRVGPRTFQDLIRHFGSARAA
                     LAALPDLARRGGSRSSPRVFTPEQAAAEIKAARTRDVEYVALGEPAYPLRLQMIDDAP
                     PLIGVRGNLDVFARPQIAIVGSRNASAAGVKFADRMARDLGAAGFVVVSGLARGIDAA
                     AHRASLPTGTIAVLAGGHGCIYPAQHVELVDNLVVDGAAISEMPPAWEPRAHDFPRRN
                     RLISGLSIGVIVVEAARRSGSLITARLAGEQGREVFAVPGSPLDPRCEGTNGLLKQGA
                     IPVTEVSDVIAVVEPILGRDVELAGVREPAQDALQKNIEPDADDRSKIVSLLGPTPVS
                     IDDLVRFAQSSPAIVRAALLELEIAGRIERHGAGLVSLV"
     gene            2971..4155
                     /locus_tag="VFL49_08435"
     CDS             2971..4155
                     /locus_tag="VFL49_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET7912044.1"
                     /translation="MTSQFFLAIAAMLIFLAPSRAQDQPVSVFLSALWTDAQAKGISR
                     ATFDTAMKGFVPDQRVIAATRRQPEYGKPVGDYVNAIVSKRRIADAQFKGREWAKTFD
                     AVEKKFQVERWVLLALWGMESNFGSEKDRWDVFRSLATLAYVKYRDPYFRNELMVAMR
                     IMQDNRIAREKMVSSWAGAMGQTQFMPSNFVDYAIDFSGDGRADIWGNVPDVLASTAN
                     YLRKWKWNPALPWGFEVLVPDGFDYMRSRANFAEWRALGMRRADGKSFPGSGQGILFF
                     PSGAGGPGFIVTENFDVLKEYNNSDAYAIAVGHLADRIHGGVTIKTPWPRDDRQLSRD
                     ARVALQKRLAALGYKVNEFEGHIDFDLRDNIRAEQKKLGIVPDGTPTPALLEKLGVSP
                     RH"
BASE COUNT          746 a         1322 c         1282 g          827 t
ORIGIN      
        1 gcgccgccgg cgtgccggaa ggcaccgatg tcgtagactg ccgcggcaag ctcgtcgccc
       61 caggcctgat cgacatgcgc gccttcatcg gcgagccggg cgaagccagc cgcgagacgc
      121 tcgcttcggc cagccaggcg gccgcggccg gcggtgtcac cacgatcatc tgccagccgg
      181 atacgtcgcc cacaatcgat gatccggcga ccgtcgattt cgttctgcgc cgcgcgcgcg
      241 acaccgggat cgttcacgtg catccgatgg ccgcgatcac caaaggcctc gagggcaaag
      301 agatgacgga gatcggcctg ctcaaggcgg ccggagccgt ggctttcacc gacggcgcca
      361 gaagcatcac caacgcacag gtgatgcgtc gtgtcctgac ttacgccggc gacttcgacg
      421 ccttgattgt tcatcacgtc gaagacccga atctggtcgg tgacggcgtc atgaacgaag
      481 gcgaattcgc ggcccggctc ggccttctcg gaattcccaa gaccgcggag acgatcatgc
      541 ttgagcgcga cacgcgtctt gtcgcgctgg caggcggccg ctatcacgcc gcggctgtca
      601 cctgcgtgga gacgctcgag gttctccgcc gggcgaagga cgcaggcctg agcgttacag
      661 cttcggcctc gatcaatcac ctcaccctca acgagattga tatcggccct taccgtacct
      721 tcctgaaact ttcgccgccg ctgcgcgccg aggatgaccg caaagcattg gtccaggccg
      781 tcgcagacgg cgtcgtcgat gtggtggtat cggatcacaa tccgcaggac gtggagacaa
      841 agcgcctgcc ctttgcggag gcagcgcccg gcgcgatcgg tctcgaaacc atgctcaccg
      901 cggggctgcg gctcgtccat aatggcgaga ttgaactcgt gcccctgttg cgcgcgatgt
      961 ccacgcgccc ggccgagttg ctgggccttc ccggcggcac gctcagaagt ggcgcgcccg
     1021 ccgacgtgat tgtcgtcgat accgaactcc cctgggtcct cgacccgaat gacctcaaat
     1081 cgaagtgcaa gaacacgcca ttcgacgagg cacggcttac cggccgcgtc gtgcgtacga
     1141 ttgtcgccgg gcgaaccgtc tacgaatacg tatgattgcc gccgctgcct ttgcgttcgg
     1201 atacctgctc ggctcgatcc cgttcggact attgttcacg cgcgccgccg gcggcccaga
     1261 catcagggca atcgggtcag gcaatatcgg cgcgaccaac gttctgcgca cgggccacaa
     1321 ggccctcgct gcggcgacgc tggtctgcga catgctcaaa ggcgccatcg cggttcttgc
     1381 cgtggcgcat tatggcgccg atgccgctat tgccgccgca cttggtgcgt tcctaggcca
     1441 cctttttccg gtctggctga aatttaaagg cggcaagggg gttgccactt ttatcggcct
     1501 tctcattgga ctttattggc cggcggccct ggtcttttgc atcgtctggc tcgccgtagc
     1561 tgccatcagc cgttattcct cgctggcagc tcttgtcgcg agtgcgatca caccgcttgc
     1621 ggtctggtac ttcggtctgc cgcgcgtcgc ggctttattc ctgctgctca cggttttgct
     1681 gtgggtcatg caccgcgcca acattgcgcg gctgctgaat ggcagcgaag gcaagatcgg
     1741 cgcgcaaggt gtcggcatca attaaaccaa cggttgtatt ttagcaaaat cggttgtact
     1801 gctgccgcgt gtgaatgcgg actgggtggc acgttgcaac agcggttgag tgacgccgag
     1861 cggctcgatt ggcttcgcct cattcgaacg gacagggtgg gtccgcgtac gtttcaagat
     1921 ctgatccggc acttcggcag cgctcgggct gcgcttgccg cacttcctga tctcgcgcgc
     1981 cgcgggggat cgagatcttc gccacgcgtc ttcacccccg agcaggccgc ggcagaaatt
     2041 aaggccgcac gcacgcgaga cgtcgaatac gtggccttgg gagagcccgc ctatccgctg
     2101 cgtctccaga tgatcgacga cgcacctcca ttgatcggcg tgcgcgggaa tctcgacgtc
     2161 ttcgcgcgac cgcagatcgc aatcgtcgga tcccgcaatg cttccgccgc gggcgtgaaa
     2221 tttgccgacc gaatggcgcg ggatctcgga gcggctggct ttgtggtcgt ctccggtctc
     2281 gcacgcggca tcgacgccgc cgcccatcgt gccagcctgc ccacaggcac catcgctgtt
     2341 ctcgccggcg gccatggctg catctatccg gctcaacacg tcgagctggt ggacaacctc
     2401 gtcgtcgatg gcgctgctat ttcggagatg ccgcctgcct gggaaccgcg cgcccacgac
     2461 tttccgcgac gcaaccgatt gatctccggc ctttcgatcg gcgtcatcgt tgtggaagcc
     2521 gcacggcgat caggctcgct gatcaccgcc cggctcgccg gggagcaggg ccgtgaggtc
     2581 tttgcggtgc ccggttcgcc gctcgaccca cgctgcgagg gcacgaacgg cttgctcaag
     2641 cagggagcca ttcctgtgac cgaggtgtcc gatgtgatcg ctgtcgttga gccgatcctc
     2701 ggtcgtgacg tcgaacttgc cggagtacgg gaaccggcac aggatgcatt gcagaaaaat
     2761 atcgagccgg acgccgacga tcgcagcaag atcgtctctc ttcttggacc gacccccgtc
     2821 tcgatcgacg acctggtgcg ctttgcgcaa agctcgcccg cgatcgtccg ggctgcgttg
     2881 ttggagctcg aaattgccgg gcggatagaa cgtcacggcg cggggttggt gtcactggtg
     2941 taacgtgcga tacccgaccc ggatgttgtc atgaccagcc aatttttcct ggccatcgcg
     3001 gccatgctga tattcctggc gccgtctcgg gcgcaggacc aacccgtttc ggtttttctt
     3061 tcggcgttgt ggacggacgc gcaggcgaaa ggcatcagcc gcgcgacctt cgataccgcc
     3121 atgaaaggat tcgttcccga tcagcgggtc atcgccgcga cccggcgtca gccagaatac
     3181 ggcaaaccag tcggcgacta tgtgaacgcc atcgtatcga aacgtcgcat cgcggacgca
     3241 caattcaagg gccgcgaatg ggcaaaaacc ttcgacgcgg tcgaaaagaa atttcaggtc
     3301 gaacgctggg tgctgcttgc tttgtggggc atggaatcga acttcggttc agaaaaagat
     3361 cgctgggacg tgtttcgatc gctcgctacc ctggcctacg tgaaataccg cgatccttat
     3421 tttcgcaacg agctgatggt ggcgatgcgc atcatgcagg acaatcggat cgcgcgcgaa
     3481 aaaatggtca gctcatgggc cggcgccatg gggcaaaccc aattcatgcc gtccaacttc
     3541 gtcgactatg cgatcgattt ctccggcgac gggcgcgcgg acatctgggg caacgttccg
     3601 gatgtgcttg cctccaccgc gaactatctg cgcaagtgga aatggaatcc ggcccttccc
     3661 tggggcttcg aagttctagt gccggacgga tttgactaca tgcgcagccg cgcgaatttt
     3721 gcggaatggc gtgcgctagg tatgcgccgc gccgacggca agtcatttcc cggctccgga
     3781 cagggcatcc ttttctttcc cagcggagcc ggcggtccgg gtttcatcgt tacggaaaac
     3841 ttcgacgtgc tgaaggagta caataactcg gatgcctacg cgatcgccgt cggtcacctt
     3901 gccgaccgaa tccacggcgg tgtcacgatc aagacgccgt ggcccaggga cgatcgtcag
     3961 ctctcgcgcg atgcccgcgt tgccttgcag aaaaggctgg ccgctctcgg ctacaaggtg
     4021 aacgagtttg agggtcacat cgactttgac ttgcgcgaca acattcgcgc cgagcaaaaa
     4081 aaactaggca tcgttccgga cggcacccca acgcccgcac tccttgaaaa gctcggcgtc
     4141 tctccacggc actaggagtg ccattgaaac tggcatc
//
LOCUS       DASSRL010000415         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_203280, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000415 DASSRL010000000
VERSION     DASSRL010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203280"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFL49_08440"
     CDS             complement(<1..293)
                     /locus_tag="VFL49_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912045.1"
                     /translation="MSDTALAAQPVRVAAPLRPWYLVAAAAALTVMIAAILSPSLWFL
                     NFVHVMTGALWTGIDLFMGFVIGPILRGVSFETRRNIIAGLIPRTLILMPTLS"
     gene            428..1396
                     /locus_tag="VFL49_08445"
     CDS             428..1396
                     /locus_tag="VFL49_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912046.1"
                     /translation="MRRREFIILSAGAAVWPFAARAQEKRAHRIGILSQDLQPGLLET
                     FRDELHKRGYVEGGNINIELRNAAGHNERLPVLAEELLKLKVDVILAVNTPAAQAAKK
                     ATKIVPVVMMRVADPVRSGLVASLARPGGNITGLSFMPEALGPKGVELLHEMLPKIRR
                     VAALYQGNNPGAVIIIDDVQRKGEQIGLNFVRLPVKGGQDLPAAFEAAAKAKAEALFV
                     MDDGAMTEQRQGILELAEKQHLPVVSIYRDFAASGGLIAYGPNLDVVYRRAAVYVDKL
                     IKGAPAPSLPVEQPVNFYLAVNLKTAKTLGVTIPPSILVRADEVIE"
     gene            complement(1525..2841)
                     /locus_tag="VFL49_08450"
     CDS             complement(1525..2841)
                     /locus_tag="VFL49_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7912047.1"
                     /translation="MSEEWIGILSLAILFGAIFIGFPIAFTLIAVALGVGYWALGPIA
                     LHLMTIQFFAVMKETSLASVPFFLFMGFLLEQSGLMERLFRGIQQLLAGVRGSLYLAV
                     LITATIFAAATGIVGSSVTLLGVMAAPSMTRSKYDVRLSAGAITAGGTLGILIPPSVM
                     LIVMGPVVGVPTTDLFAAAVFPGLVLSGLYIGYCLARSYIDPSVGPPLRQDERLASKA
                     AVLKELTLGVVPVVIVIMATLGAILAGVATPTDAGACGAFVVLVMTVISRTITWQKLK
                     NSVYATLEVSCMILLLVAASNYFGAVFSRLGSANMIAEALLNLPLPPMIMLILILGVI
                     FLLGWPLEWVPIVLIVVPILLPLVQKLGIDLIWFCTLVAVCLQTAWLSPPVALSAYFL
                     KGVVPDWKLSDIYAGMMQFMVLQVIGLGLVLLFPQIATWLPAVLRN"
     gene            complement(2834..>3473)
                     /locus_tag="VFL49_08455"
     CDS             complement(2834..>3473)
                     /locus_tag="VFL49_08455"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET7912048.1"
                     /translation="CGSPRPWASVYFLHKAAGQGVVMTMQASPGLVRAVNFFDGISLW
                     SGRIVAWLILPMVMSLVWEVFARYFFNAPTIWAYDMTYILYGSFFMLGSAYTLLRGKH
                     IRTDNFYGQWPVRRQGMVDAVCYVVLFFPPLLALLYVTWDYFWVSFMRGERVVSSPWM
                     PIIYPLKFTMPVTCLLLMLQGVAEFLRSIHAARTGVWLPRPSLMPERVVADV"
BASE COUNT          807 a         1058 c         1080 g          528 t
ORIGIN      
        1 atcgacagcg tcggcatcag gatcagcgtg cgcgggatca atccggcgat gatattgcgc
       61 cgcgtctcga atgatacgcc gcgcaggatg gggccgatga cgaagcccat gaacaggtcg
      121 atgccggtcc acaatgcgcc ggtcatcacg tgcacgaaat tgaggaacca gaggctgggg
      181 ctgaggatcg cggcgatcat aaccgtcagc gcggcggcgg ccgcgacgag gtaccagggc
      241 cgcagcggag cggcgacgcg gacaggctgg gccgccagtg cggtgtcgga caaaatgccg
      301 gttcctctca gcgattccga taaaaccgta gcggcggatc gttgcgttgc caagcgaact
      361 cccggcgccc tctttcaacc ggatcagtta acccattaca atgccggacc ggggctgggg
      421 gacgtccatg aggcggcgcg aattcatcat actgtccgcc ggcgcagcgg tttggccgtt
      481 tgcggcgcga gcacaggaaa aacgggcgca tcgtatcggc atcctctccc aggatttgca
      541 gccggggctg ctggagacgt tccgcgacga gcttcacaag cgaggctacg tcgaaggcgg
      601 caatatcaac atcgaattgc gaaatgcggc gggacacaac gaacggttgc ccgtccttgc
      661 cgaggagctt ctcaagctca aagtcgacgt gatcctcgcc gtcaatacac ccgctgcaca
      721 ggctgccaaa aaagccacca agatcgttcc cgttgtgatg atgcgcgtgg ccgatcccgt
      781 caggtcagga ctggtggcga gcctcgcccg ccccggcggg aacatcacgg gactgagctt
      841 catgccggag gctcttggac caaagggagt tgaacttctt cacgagatgc ttcccaaaat
      901 caggcgcgtg gccgcgctct atcagggaaa caacccgggc gccgtcatta tcatcgacga
      961 cgtccagcgc aagggcgagc agattggact caatttcgtg cggctcccgg tgaagggcgg
     1021 gcaggacctt ccagccgcct ttgaggctgc agcgaaagcc aaagctgagg cgctctttgt
     1081 catggatgac ggtgcaatga ccgaacagcg ccagggaatt ctcgagcttg cggaaaagca
     1141 acatctgccg gtggtgtcga tctaccggga ctttgcggcc tccggcgggc tcatcgcgta
     1201 tgggccgaac cttgacgtcg tgtaccggcg cgcggcagtt tacgtcgaca agcttataaa
     1261 aggcgcaccg gcgccgtctc ttccggtcga acagccggtc aatttctatc ttgcggtcaa
     1321 cctcaagacc gccaagaccc tcggcgttac gattccgcca tcgatattgg tccgggccga
     1381 cgaagtgatc gaatagaaca cgtcaattcg cagttccggc ggcgtaggat gggttgagcg
     1441 tcagcgaaac ccatcctacg gccacaaccg cttcgtagcg tgggttgagc gacagcgaaa
     1501 cccacgattg aatgaggagc gctctcagtt tctcagcacc gccggcagcc aggttgcgat
     1561 ctgcgggaac agcagcacca gcccaagccc gatcacctgc aagaccatga actgcatcat
     1621 cccggcgtag atgtcgctga gcttccaatc cggcacgacg cccttcagaa aataggccga
     1681 caaggccacg gggggtgaaa gccaggcggt ctgcaggcac accgcgacca gcgtgcagaa
     1741 ccagatcagg tcgatgccga gtttctgcac cagcggcaga aggatgggca cgacgatcaa
     1801 cacgatcggt acccattcca gcggccagcc gagcaaaaaa atcaccccca ggatgaggat
     1861 cagcatgatc atcggcggca gcggcaggtt gagcagtgct tcggcgatca tgttggccga
     1921 gcccagccgg gagaacacgg cgccgaaata attggaggcc gccaccagca gcaaaatcat
     1981 gcacgacact tcaagcgtcg cgtagaccga attcttgagc ttctgccagg tgatcgtgcg
     2041 cgagatgacg gtcatcacca gaacgacgaa ggccccgcag gcgcccgcat cggtcggcgt
     2101 ggcgacgccg gcaaggatgg cgccaagcgt cgccatgatg acgatgacca ccggcaccac
     2161 gccgagcgtg agctccttga gcaccgcagc cttggaggcc agccgttcgt cctgcctgag
     2221 aggcggaccg accgaaggat cgatatagct gcgcgccagg caatagccga tgtagaggcc
     2281 ggacagcacg aggccgggaa aaaccgcggc ggcgaaaaga tcggtcgtcg gcacgcccac
     2341 caccggaccc atcacgatca gcatcaccga cggcggaatg agaatgccca gcgtaccgcc
     2401 cgcggtaatt gccccggccg aaagccgtac gtcatacttg ctgcgcgtca tgctgggcgc
     2461 cgccatgacg ccgagcagcg tcaccgagga gccgacgatg ccggtcgccg cggcgaagat
     2521 cgtggcggtg atgaggacag cgagatagag cgagcctctt acgccggcga gcagctgctg
     2581 aatgccgcgg aacagccgct ccatgagccc cgactgttca aggagaaaac ccatgaacag
     2641 gaaaaacggc acggaggcga gcgaggtctc cttcatcacc gcaaagaact ggatggtcat
     2701 gaggtgcagc gcgatcggcc ccagcgccca gtaaccgaca ccgagggcga ccgcgatgag
     2761 cgtgaacgcg atcggaaagc cgatgaagat tgccccgaac aggatcgcca gtgaaagaat
     2821 gccaatccat tcttcagaca tcggccacca cccgctcggg catcaatgat ggccgcggca
     2881 gccacacgcc ggtcctcgcg gcatgaatcg accgcaaaaa ttcagcgacg ccctgaagca
     2941 tcagcagaag gcaggtcacc ggcatggtga atttcagcgg gtagatgatc ggcatccagg
     3001 gacttgagac gacgcgttcc ccgcgcatga aggaaaccca gaagtagtcc caggtcacat
     3061 agagaagcgc gagcagcggc ggaaagaaca gaacgacgta acagaccgca tcgaccatcc
     3121 cctgccgtcg caccggccac tggccgtaga agttgtcggt ccggatatgc ttgccgcgca
     3181 gcagcgtgta ggcggatccg agcatgaaaa acgagccgta gaggatgtag gtcatgtcgt
     3241 aggcccagat ggtcggcgcg ttgaagaagt agcgcgcgaa cacctcccac acgagactca
     3301 tgaccatcgg gaggatgagc caggcgacga tccggcccga ccacaaactg atcccgtcga
     3361 agaaattcac ggcgcgcacg agaccaggcg atgcctgcat cgtcatgacg accccctgcc
     3421 ccgcggcttt atgcaaaaag tagacggaag cccacggacg cgggcttccg cag
//
LOCUS       DASSRL010000416         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1463114, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000416 DASSRL010000000
VERSION     DASSRL010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463114"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFL49_08460"
     CDS             <1..359
                     /locus_tag="VFL49_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7912049.1"
                     /translation="SEAVKKFGQMLVDDHGKALEKAKAAAGQAGVEPPSGSSLTQKGT
                     YAKLKLLQGEAFDKSFANAMVSDHQNDIKEYEKAAGKNNAVGHYAKEALPTLRTHLQH
                     AKALQQELKRQTTGSR"
     gene            complement(639..950)
                     /locus_tag="VFL49_08465"
     CDS             complement(639..950)
                     /locus_tag="VFL49_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912050.1"
                     /translation="MSGKVGLIALLAAASLLLLWRHLTTQSTNEPAIGFGYSSEQISK
                     AREVCTRLHGSEQERRQRLADCNGECGNLVSDASRACLSQCREKSQAYASCLLQYTTP
                     P"
     gene            complement(1101..1298)
                     /locus_tag="VFL49_08470"
     CDS             complement(1101..1298)
                     /locus_tag="VFL49_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912051.1"
                     /translation="MVAKNSAAKKKNKSELYHATLLVTRAEEWCVEASSPEDAKARLQ
                     RGEGHRCSPGEIFAVELGDIL"
     gene            1388..2257
                     /locus_tag="VFL49_08475"
     CDS             1388..2257
                     /locus_tag="VFL49_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7912052.1"
                     /translation="MAPRAYWKGFLRLSLVSCPIALFPATSEREKIHFHQINRKTGNR
                     IQYRKVDAETGREVDRDDIIRGYEKSKGDYVPVEPEELEAVALESKRVIEIDEFVPRK
                     EIDELYFANPYYIVPDGEAGAQAYAVIRESIEQANMMALGRVVFTSREHVIALEARGK
                     GIMGVTLRYPYEVRDEQDYFDDIPGERVTKDMLELAAHIIKTKTGHFDPGKFEDQYED
                     ALKELLRKKDKGEKIEPPRQREEGNVVNLMDALRRSVKGERAAPRRTRHHHAPDRRKG
                     AKKRPAARHRKAG"
     gene            2388..2687
                     /locus_tag="VFL49_08480"
     CDS             2388..2687
                     /locus_tag="VFL49_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912053.1"
                     /translation="MKSYKILDIVGRVYDNVEIALWAILLAFVVFMAVFVVPMLPKLR
                     AQYQEARARQIAEETALYCQKLNVKAGTQGYGHCLLVLGEFRHKVEQRIYSESEF"
BASE COUNT          578 a          835 c          874 g          474 t
ORIGIN      
        1 agagcgaggc cgtgaaaaaa ttcggccaga tgctggtcga tgaccacggc aaggcgctcg
       61 agaaagccaa agcggcggcc ggtcaggccg gcgtcgagcc accgagcggg tcgagcctca
      121 cgcagaaggg aacctacgcc aagctgaagc ttttgcaggg cgaagccttc gacaagtcgt
      181 tcgccaatgc gatggtctcc gaccaccaga acgacatcaa ggaatatgag aaggccgccg
      241 gcaagaacaa tgcggtcggt cactatgcga aggaggcgct gccgacgctg cgcacccatc
      301 tgcagcacgc gaaggcgttg cagcaggaac tcaagcggca gaccaccgga agcaggtaac
      361 tctctgaaac cgtgcgaagc ctcggttgaa cgatccgcct gaattcgcgg cgcgcttcct
      421 cagcgaacgg ccaacggatg gcgacaatcg gatgctccgg ccgtcttacc aagcatcgtt
      481 cgagtacata tatgcccggg tgccggtcgc ccggccggtt ccccaagagg ctggcctccg
      541 tcccccgact aacagcctcg ccgccgacga ccggcactgg cttgctttgt gcaccgcaaa
      601 cgcgcctgaa taatggcgca aaggtgacgc gccgccggtc agggcggcgt cgtgtactgc
      661 agcaggcagc ttgcataagc ttgcgacttc tcgcggcatt gcgacaggca tgcccgcgag
      721 gcatcgctca ccagatttcc gcattcgccg ttgcaatcgg cgaggcgctg ccgtcgctcc
      781 tgttcggagc cgtgcaacct ggtgcagacc tcgcgcgcct tggagatttg ttcagacgag
      841 tagccaaagc cgatggccgg ctcgttggtg ctttgcgtgg tgaggtgccg ccacagcagc
      901 agcagactcg ccgcggcgag aagcgcgatc aaaccaacct tgcctgacac gaccttcggc
      961 ccgttccgtt ctctgaacgg cggcttgcat cggtcgcccc gccgccaatg accgggcgta
     1021 tggctgcgcc cgcggcggtc gcctgtcaat cccggctgcg gctcgcgtcg ggccaagctg
     1081 tctgtcaaag gacaaaaggc tcaaaggata tcgccgagct ccacagcgaa gatttcccca
     1141 ggcgagcagc ggtggccttc gcccctttgc aggcgcgctt tggcgtcctc cgggctggat
     1201 gcctccacgc accattcctc ggcgcgggtg accagcagcg tggcgtgata gagctcgctc
     1261 ttgttcttct tcttcgcggc cgaatttttc gcaaccatga cggaatgaat ccgcgaatca
     1321 gcagatttgt tccggaacgc cggcggagag ggggcgttga cgtccgtcat cttgacggag
     1381 ttccggcatg gccccgcgcg cctattggaa aggttttctg cggctgtcac tcgtttcctg
     1441 cccgatcgcg cttttcccgg cgacgtccga gcgggagaag attcacttcc atcagatcaa
     1501 caggaaaacc ggaaaccgca tccagtaccg gaaggtcgat gcggaaacag ggcgcgaggt
     1561 cgaccgcgac gacatcatcc gcggctacga gaaaagcaag ggcgactacg tgccagtcga
     1621 gcccgaggag cttgaggcgg ttgcgctcga gagcaagcgt gtgatcgaga tcgacgaatt
     1681 cgtgccgcgc aaggagatcg acgaactcta tttcgccaat ccctattaca tcgtcccgga
     1741 tggcgaagcc ggggcccagg cctacgcggt gatccgggag tcgatcgaac aggcgaacat
     1801 gatggcgctg gggcgcgtgg tgttcacttc gcgcgagcat gtgatcgcgc tggaagcgcg
     1861 gggcaagggc atcatgggcg tgacccttcg ctatccctac gaggtgcgcg acgagcagga
     1921 ttacttcgac gacattccgg gtgaacgcgt gaccaaggac atgctcgagc ttgcggcgca
     1981 catcatcaaa accaagacgg gccacttcga tccgggcaag ttcgaggatc agtacgagga
     2041 tgcactgaag gagcttctgc gcaagaagga caaaggcgaa aaaatcgagc cgccgcggca
     2101 gcgcgaggag ggcaacgtcg tcaacctgat ggatgcgctg cgccgcagcg tgaagggaga
     2161 acgcgcggcg ccaagacgca cgcgccacca ccacgccccc gatcgccgca agggcgcgaa
     2221 aaagcgaccg gccgcgcgcc atcgaaaggc gggctgagcg gccggccggg tgacacgaat
     2281 tcgtccggat tctataaaga cccacctata gagtgtgacg cgcttcacat ttgaaaattg
     2341 ccgcgtgcac tagattgccg actggaccgc cggtaggggg cgaggccatg aaatcgtaca
     2401 aaattttgga cattgtcggc cgcgtttacg acaacgtgga aattgctttg tgggcgatcc
     2461 tgcttgcctt tgtcgtgttc atggcggtgt ttgtggtccc aatgttgccc aagcttcgcg
     2521 cgcagtatca ggaagcgcgg gcgcggcaga tcgccgaaga gaccgcgctc tattgccaaa
     2581 agctgaacgt gaaagcggga acgcagggtt atggccactg tctgctcgtt ctcggcgaat
     2641 ttcgccacaa ggtcgagcaa cgcatctaca gcgagagcga gttctgaccg gcgtccggtc
     2701 cgaagcctcc aggaaagcgg ccgcgccgga gaataatccg gcgtggcctc tcacacatcg
     2761 a
//
LOCUS       DASSRL010000417         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1130985, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000417 DASSRL010000000
VERSION     DASSRL010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130985"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFL49_08485"
     CDS             complement(<1..638)
                     /locus_tag="VFL49_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7912054.1"
                     /translation="MTVDTFSMRSLSRRSLLKTAAALPLVYAAPLRAAAPAAETITPA
                     LVAAATKEGQVTWYTSADLQLAEKVGKAFEQKFPGIRARVERAGGERIFSRVAQEYAA
                     GLHVPDAVSTGDAAQFLVWKRQGLLAPYVPDDVAKHIPAEHRDSDGFYATVRSSLCVI
                     AYNTQLVKSAEAPKSFADLLDPKWKGKIVKAHPSYSGTIMTSTYQMVRELGWG"
     gene            complement(793..>3914)
                     /gene="dnaE"
                     /locus_tag="VFL49_08490"
     CDS             complement(793..>3914)
                     /gene="dnaE"
                     /locus_tag="VFL49_08490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7912055.1"
                     /translation="AFLDTPANEPPRLKAEWLAGEIAGIIALTGGPGGPLDGALAAGQ
                     SHLAASRLDALLQLFGDRLYIELQRHGLASERRVESGLIDLAYAKSVPLVAANEPYFA
                     GAQDYEAHDALICIAEGRLLAETARRQLTQEHRFKSRSEMVELFADLPEAVTSTVEIA
                     QRCAFRPRTHQPILPRFSLGENGKELDEASELRKRAEAGLERRLATAGVADGHTVDEY
                     RERLEFELGVIAGMKYPGYFLIVSDFIQWAKKQGIPVGPGRGSGAGSLVSYALTITDL
                     DPIRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIRYVQERYGRDQVAQIITFGTLQ
                     ARGVLRDVGRVLQMPYGQVDKLCKLVPQNPAAPVSLKQAIEGEPRLQAERDSEPVVKR
                     AFAIAQKLEGLNRHASTHAAGIVIGDRPLSELVPLYRDPKSDMPVTQFNMKWVEQAGL
                     VKFDFLGLKTLTVLQTAVALVKRRGIVIDLSAIPLDDKNTYDLLARAEAVGIFQLESA
                     GMRRALLDMRPDRFEDIIALVALYRPGPMANIPTYCARKHGMEHPDYIHPKLEPILRE
                     TFGVIIYQEQVMQAAQILAGFTLGEADLLRRAMGKKIRKEMQAQRERFVSGAIERGVE
                     RNQADAIFDLLERFAEYGFNKSHAAAYALVAYQTAYMKANFPVEFLAATMTLDMGNTD
                     KLAEFRAEAERLGIAVDPPSINRSAVEFEVDGNTIIYALAALKGVGRQAVEAIMAARE
                     SGPFGDLADFAGRINPRAVNKRVLESLAAAGAFDAFDKNRARIFAAVDAVLGRAQRSH
                     DTAAIGQSELFGGGPVREQIALPQIESWLPAERLRREYEAVGFFLSGHPLDDYASVLK
                     KLRVQPWAEFSRAVKAGATAGRVAATVVARTERRTRTGSKMGIIGLSDPTGHFEAVIF
                     SEGLAEHRDLLEPGNAVLLFLSAEVQGDEVRARIQSAEPLDNAAANLQKGLRVFLRDE
                     KPLDGVAKRLEAVPRSAKPNGDGEVSVVLLLQQGTEVEVKLPGRFKVSPQIAGAIKAV
                     PGVVQVEAL"
BASE COUNT          734 a         1256 c         1203 g          721 t
ORIGIN      
        1 ccccatccaa gctcgcgcac catctgatag gtcgaggtca tgatggtgcc gctgtagctc
       61 ggatgcgcct tgacgatttt tcctttccat ttcggatcga gcagatcagc aaaacttttt
      121 ggcgcttcgg ccgatttcac aagctgcgtg ttgtaggcga tcacgcacaa tgacgacctc
      181 accgttgcgt aaaagccgtc agaatcgcga tgctcggccg ggatgtgctt tgcgacatcg
      241 tccggaacgt aaggtgcaag cagaccctgc cgcttccaca cgaggaattg cgccgcgtcg
      301 ccggtgctca cggcgtcggg cacatgcagg ccggcggcat actcctgcgc cacgcgcgag
      361 aagatgcgtt caccgccggc gcgctcgacc cgcgcgcgga tgccgggaaa tttctgctcg
      421 aacgccttgc cgactttttc cgcgagctgg aggtccgcgg acgtatacca ggtgacctgc
      481 ccttctttcg ttgccgcggc gacaagcgcc ggtgtgatcg tttcggccgc aggtgccgcg
      541 gcccgcaaag gcgcggcata aacgagcgga agcgccgcgg cggttttgag cagggagcgg
      601 cgggagagcg agcgcataga aaaagtgtcc actgtcatgg ccgggcttgt ccggccatcc
      661 gcttagggag gcaccatgct cacccaagca gggtcacatt ggaagtcggg cttgcccgat
      721 ttccaatctt tctaacgccg caagccgggt aaacccgagt tgcggcgaca agcccgacga
      781 cgacagagaa aactacagcg cctcaacctg cacgacgccc ggcacggcct tgatggcgcc
      841 ggcgatctgc ggcgagacct tgaagcggcc aggcagcttg acctcaactt cggttccctg
      901 ctgcagcagc aggacaacgc tcacttcgcc gtcgccgttc ggcttggccg atcgcggaac
      961 cgcctcaagc cgctttgcaa cgccatcgag cggcttttca tcgcgcagga atacgcgcag
     1021 gcctttctgc agattggccg cggcgttatc gagcggttcg gccgactgga tgcgcgcgcg
     1081 cacctcgtcg ccctgcactt ctgccgaaag aaacagcagg acagcgttgc cgggttccag
     1141 caagtcccga tgctcggcta acccttccga gaaaatcacc gcctcgaaat gcccggtcgg
     1201 gtctgacagg ccgatgattc ccatcttgct gcccgtgcgc gtccgccgct cggtgcgcgc
     1261 gacgaccgtt gcggcaacgc ggccggcggt ggcgccggcc ttgaccgcgc gcgaaaattc
     1321 cgcccagggc tgaacgcgca gcttcttgag caccgacgca tagtcgtcga gcggatgacc
     1381 tgacagaaaa aagcccaccg cttcgtattc gcggcgcagc cgttccgccg gcagccagga
     1441 ctcgatctgc ggcagcgcga tctgttcgcg tacaggccca ccaccaaaca gttcactctg
     1501 cccgatcgcc gccgtgtcgt ggctgcgttg cgctcggcca agcactgcat cgacggcggc
     1561 aaaaatccgc gcgcgatttt tatcgaaggc atcgaatgcg ccggccgccg ccaggctttc
     1621 cagtacccgc ttgttgacgg cgcgcggatt gatccgcccg gcgaaatccg cgagatcgcc
     1681 gaacggcccg ctctcccgcg cggccatgat cgcttcaacc gcctgccgtc caacgccttt
     1741 gagcgccgca agcgcgtaga tgatcgtgtt gccgtcgacc tcgaattcga ccgccgaccg
     1801 attgattgac ggtggatcga cggcgatgcc caaccgttcg gcttcggcgc ggaattcggc
     1861 gagcttgtcg gtattgccca tgtcgagcgt catggtcgcc gccaggaact cgaccgggaa
     1921 attcgccttc atataggcgg tctgataggc aacgagggca taagccgcgg catggctttt
     1981 gttgaagccg tattccgcaa aacgctcgag caggtcgaag atggcgtcgg cctggttgcg
     2041 ctcgacgccg cgctcgatcg cgccggagac gaaacgctcg cgctgcgcct gcatttcttt
     2101 cctgatcttc ttgcccattg cgcggcgcag aagatcggct tcgccaagcg tgaacccggc
     2161 aagaatctgc gcggcttgca tcacctgttc ctggtagatg atcacgccga aggtttcgcg
     2221 caggatcggc tcaagcttcg gatgaatgta gtcggggtgc tccatgccgt gcttgcgcgc
     2281 acaataggtc gggatattcg ccatcggacc cgggcgatag agcgcaacga gcgcaatgat
     2341 gtcctcgaag cggtcgggcc gcatgtcgag cagcgcgcgg cgcatgcccg cgctttccag
     2401 ctggaagatg ccgacggctt cggcgcgagc cagaaggtcg taggtgttct tgtcgtcgag
     2461 cgggatcgct gagagatcga tgacgatacc gcggcgcttc accaatgcga cagccgtctg
     2521 cagcacggtg agcgtcttga gaccgaggaa gtcgaacttc accagccccg cctgctccac
     2581 ccatttcatg ttgaactggg tgaccggcat gtcggatttc ggatcgcggt agagcggcac
     2641 caattccgac aagggtctgt cgccaatgac gataccggcg gcatgggtcg acgcgtgacg
     2701 attgagaccc tcgagcttct gcgcgatggc gaaggcccgc ttgaccaccg gctcgctgtc
     2761 acgttcggcc tgaaggcgcg gctcgccttc gattgcctgc ttcagcgaca ccggcgccgc
     2821 cggattctgc ggcaccagct tgcacagctt gtcgacctgt ccgtacggca tctgcaggac
     2881 gcggccaacg tcacgaagaa cgccccgcgc ctgcaacgtg ccaaaggtga tgatctgtgc
     2941 cacctggtcg cgcccgtacc gttcctgcac gtatcggatc acttcatcgc ggcggtcctg
     3001 gcagaaatcg atgtcgaaat cgggcatcga gacgcgctcg ggattaagaa accgctcgaa
     3061 cagcaagccg aagcggattg gatcaagatc ggtaatggtg agggcatatg acaccaacga
     3121 tcccgcgccc gatccacggc caggaccaac gggtatcccc tgtttcttcg cccattggat
     3181 gaaatcggaa acgatcagga agtagcccgg atatttcatc ccggcgatga cccccagttc
     3241 gaactcgagc cgttcgcggt attcatcaac ggtatgacca tcggcgacgc ccgcggtcgc
     3301 gaggcggcgc tcgagcccag cttccgcgcg cttgcgcaac tcgctcgcct catcgagctc
     3361 tttgccgttt tcgccgaggg agaaacgcgg aaggatgggt tggtgcgtgc gcggtcggaa
     3421 tgcacagcgc tgcgcgatct cgaccgtcga agtaaccgct tccggcagat cggcaaagag
     3481 ctcgaccatt tccgagcgag acttaaaccg atgctcctga gtcagctgac gcctggctgt
     3541 ctccgcaagc agccggcctt cggcaatgca aatcagtgca tcatgcgcct cgtaatcctg
     3601 cgcacccgca aaatacggct cgttggcagc gaccagcggc accgatttgg cgtaagcaag
     3661 atcaatcaag cctgattcga cgcgccgctc cgacgccagc ccgtgacgtt gcaattcgat
     3721 atagagacgg tcgccaaaca gctgcagcaa tgcgtcaaga cggctggccg ccagatggct
     3781 ttgtccggcg gcgagcgcgc cgtcgagcgg cccgcccggt ccgcccgtga gcgcaatgat
     3841 gccggcgatc tcgccggcca gccattcagc tttgaggcgt ggcggctcat tcgcgggggt
     3901 gtccaggaag gcgc
//
LOCUS       DASSRL010000418         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_868503, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000418 DASSRL010000000
VERSION     DASSRL010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868503"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFL49_08495"
     CDS             <1..889
                     /locus_tag="VFL49_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7912056.1"
                     /translation="AQPWSVDPHAGAIVDGKLYGRGSSDMKSGVAAFVVACLQLSSKL
                     VGTPGVVLVITAGEETGCTGAAALAQEFKNLPKVGAFVVAEPTGNRPLVGHKGALWVE
                     AVTKGLTAHGSMPEKGVNAIYKAARAVTALQDFDFNVARHDVLGGPTLNVGTITGGLN
                     INSVPDRTAIGIDIRTIPGQNHAGIRDQLASYLGADVELGTKLDAQSVWTNPNDAWIG
                     TVVRTAREIAGVGGEVGAAPYFTDASVLTPALGGPPTAIIGPGELELAHQTDEFCFVS
                     RIEQATDIYATIIRDWCAL"
     gene            complement(886..1722)
                     /locus_tag="VFL49_08500"
     CDS             complement(886..1722)
                     /locus_tag="VFL49_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET7912057.1"
                     /translation="MPRHIVCLTFDFDTQSGFIARGMTTPTPLSRGEFGALASRRILD
                     FLGSRAIKATWFIPGFTIESWPRECEAVVKAGHEVAHHSWAHVPPATQSRDEEEADLV
                     RANEAIARLTGKKARGYRSPSWDLSDHTVDLLLAHGFTYDSSLMGSDYVPYRARRGDT
                     ARLGEPYHFGEETRLIEMPISWSLDDYPHFEFVRTQASVLAGMQPARAVLQNWFDEFL
                     YMKKAVDWGILTYTMHPYVIGRGYRMLALEDMVDRLAKEGATFMTMEDAAREAEEHLP
                     KR"
     gene            1803..>2884
                     /locus_tag="VFL49_08505"
     CDS             1803..>2884
                     /locus_tag="VFL49_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7912058.1"
                     /translation="MAVKDIGIIVNGATGRIASTQHLANALLPIRAEGGLAVEGDRIV
                     PRLLLSGRDEKRLATLARSNGIDRWTVDLDAALSDPQFSVFFDAAATVQRVPTLMKAI
                     AAGKHIYSEKPVALSVSEGISLLTAARARNLRHGAVEDKIFLPGLQKLAQLAERKFFG
                     RVTGFRLDFGWWVFDGFERKSQRPSWNYRRSGAGGLIVDMYPHWRYVIENILGPIRRV
                     VVVTATAVPQRVDECGARYDVDVDDTSLTLAELHNGAVGTIACSWATRVRGDDLLTFQ
                     IDGTRGSAIAGVHRCWAQSAGDTPDVRHINPTVDIDFDYRRDWKEVPASAAHINPYRV
                     GWEQFLRHIVAGEPLASDLSAGIRDV"
BASE COUNT          529 a          870 c          930 g          555 t
ORIGIN      
        1 cgcgcagcca tggtcggtcg atccgcacgc cggggctatt gtcgacggga agctctatgg
       61 ccggggctcg tcggatatga aaagcggcgt cgcggccttt gtcgtcgcct gcctgcagct
      121 gtcaagcaag ctcgtgggta cgccaggcgt ggttctggtc atcacggcgg gagaggaaac
      181 cggctgcacc ggcgccgcgg cgcttgcgca ggaattcaaa aacctgccga aagtcggcgc
      241 gtttgtcgtg gcggagccga ccggcaaccg gcctttggtc ggccataaag gcgcgctgtg
      301 ggtcgaggcc gtcacgaaag gactgaccgc ccacggctcg atgccggaaa aaggcgtcaa
      361 tgcgatctac aaggccgcgc gcgcggtcac ggccctgcag gatttcgatt tcaacgtcgc
      421 gcgccacgat gtgctcggcg ggccgacatt gaacgtcggc accatcaccg gtggtctcaa
      481 tatcaattcg gtgccggacc gcacggccat cggcatcgat atccgcacca tacccggaca
      541 gaaccacgca ggaatccgcg atcagctcgc gagctatctc ggcgccgacg tcgagcttgg
      601 caccaagctc gatgcgcaga gcgtatggac caatccgaat gatgcctgga tcgggacagt
      661 cgttcgcacc gcccgcgaga tcgccggtgt cggcggggag gtcggcgccg caccatattt
      721 taccgatgct tcggtcctga cgccggcgct gggcggaccg ccgacggcga tcatcggccc
      781 gggagagctt gaactcgccc atcagaccga cgagttttgc ttcgtgtcac ggatcgagca
      841 ggccaccgat atttacgcga cgatcatccg cgactggtgc gctctctaac gcttcggcag
      901 atgctcttcg gcctcgcgcg cggcgtcttc cattgtcatg aaggtggcac cttccttcgc
      961 aagcctgtcg accatgtcct ccagcgccag catgcgataa ccgcgcccga tcacgtaagg
     1021 atgcatggtg taggtcagga tgccccagtc gacggccttt ttcatataga ggaattcatc
     1081 gaaccagttc tgcaggactg cgcgcgccgg ctgcattccg gcgaggaccg aggcttgcgt
     1141 gcgcacgaac tcgaagtgcg gatagtcatc gagcgaccag ctgatcggca tttcgatcaa
     1201 tctggtttcc tcgccgaaat gataaggttc tccaagcctt gcggtgtcgc cgcgccgggc
     1261 gcgataaggc acatagtcgg atcccatcag gctggaatcg taagtgaagc cgtgcgccag
     1321 cagcaggtcg acggtgtgat cgctcagatc ccatgatggc gagcggtatc cgcgggcctt
     1381 tttgccggtc agacgtgcga tcgcttcgtt ggcgcgcacg aggtcggctt cctcttcgtc
     1441 gcggctttga gtcgccggcg ggacatgagc ccagctgtgg tgcgcgactt cgtgtccggc
     1501 cttgacgaca gcctcgcatt cgcgcggcca gctttcgatg gtgaatcccg gaatgaacca
     1561 ggtcgccttg atagcgcgcg agccaagaaa atcgaggatg cggcgtgacg ccagtgcgcc
     1621 gaattcgccg cgtgacagcg gtgtcggtgt cgtcatccct cgtgcgatga aaccggattg
     1681 cgtgtcgaaa tcgaacgtca ggcagacgat atggcgcggc atggcattgt cactgtgcag
     1741 ccccgccgat gatactggcg ccggcgcggc atgataggaa ggcaggcggg agggtcaagg
     1801 ttttggcggt caaggacatc ggaatcatcg tcaatggagc gaccggccgt attgcatcga
     1861 cacagcatct ggcgaacgcg ctattgccga tccgcgccga gggcgggctg gccgtcgaag
     1921 gcgaccggat cgtgccgcgt ttgcttctgt ccggccgcga cgagaaacgc cttgccacgc
     1981 tcgcacgcag caacggaatt gatcgctgga cggtcgatct cgatgcggcc ctctccgatc
     2041 ctcagttttc cgtgttcttc gatgcggcgg cgacggtgca acgagtgccg acgctgatga
     2101 aagcgatcgc ggccggcaaa catatttaca gcgaaaaacc ggtggcgctg tccgtttcgg
     2161 aaggaatttc gctccttacg gccgcgcgcg cacgcaacct caggcatggt gccgtcgagg
     2221 acaagatctt ccttccgggc ttgcaaaaac ttgctcagct cgccgagcgg aaattcttcg
     2281 gccgcgtgac cggatttcgc ctggatttcg gctggtgggt gttcgacggg ttcgagcgca
     2341 agagccagcg gccgagctgg aactaccgcc gaagcggcgc cggcgggctc atcgtggaca
     2401 tgtatccgca ttggcgctat gtcatcgaaa atattctcgg cccgatccgg cgggttgtag
     2461 ttgtgaccgc gacggcagtc ccccaacgtg tggacgaatg tggtgctcgc tatgacgtcg
     2521 acgtcgatga cacctcccta acattagcgg aactgcacaa tggcgctgtt ggcacgatcg
     2581 cctgctcatg ggcaacgcgg gtgcgcggcg acgatctcct gacgttccag atcgatggta
     2641 cccgcggctc ggcgattgcc ggcgtacatc ggtgctgggc gcagagcgcg ggcgatacgc
     2701 ccgacgttcg tcacatcaat ccgacggtcg atatcgattt tgactatcgg cgggactgga
     2761 aggaagtccc cgcgtcggcc gcgcacataa atccttatcg cgtcggatgg gagcagtttc
     2821 tccggcacat cgttgccggc gagcctctcg cgagcgatct gtcggccggt atccgcgatg
     2881 tgaa
//
LOCUS       DASSRL010000419         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_588492, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000419 DASSRL010000000
VERSION     DASSRL010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588492"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFL49_08510"
     CDS             <1..258
                     /locus_tag="VFL49_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912059.1"
                     /translation="AGALSPVLTSDNVFMAIDIFAKNVIDPETGALGTTGTVGATLCT
                     TCAPPPGETPIPGALPLFTSGMGVLGFLGWRRKRRMARQAA"
     gene            570..1577
                     /locus_tag="VFL49_08515"
     CDS             570..1577
                     /locus_tag="VFL49_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET7912060.1"
                     /translation="MHLERRIAVTGGAGFLGSHLCERLLSEGAEVICIDNFFTGPRRN
                     IEHLLDHKRFELIRHDVTFPIYLEIDEIYNLACPASPIHYQHDPVQTTKTSVHGAINM
                     LGLAKRVRAKILQASTSEVYGDPNVHPQTEEYWGHVNPIGPRSCYDEGKRCAETLFFD
                     YWRQHKLRIKVARIFNTYGPRMHPNDGRVVSNFIIQALLGRDITIYGDGSQTRSFCYV
                     DDLIDGLVRLMNSPDQVTGPINLGNPIEFSMLQLADQVLALTGSRSRVVHRPLPQDDP
                     RQRQPDISKAQESLGWRPAVPLKDGLVKTIEYFETLLREQGTLQLITETRPKHVGAVA
                     R"
     gene            1831..2034
                     /locus_tag="VFL49_08520"
     CDS             1831..2034
                     /locus_tag="VFL49_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912061.1"
                     /translation="MSGQAASRRWECAYSIDQTRAQRPYANADTQAIVSASPTTILAD
                     DNGRARNEKRLRTAGKLFTDFIL"
     gene            complement(2300..>2712)
                     /locus_tag="VFL49_08525"
     CDS             complement(2300..>2712)
                     /locus_tag="VFL49_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912062.1"
                     /translation="GASLVNFLFADQTAHPDRVAVNGGNIGWLVLLGFYVTPDGQTAY
                     AFFDDTGKFTVFPDFDGDDMVVKITFAHSGANTPETPLPGALWLFGGALAGGTAFARL
                     RKQRKTVSACAKHPRDSAGSVELPLLSGGPALGA"
BASE COUNT          586 a          788 c          791 g          547 t
ORIGIN      
        1 gccggcgctc tgagcccggt tttgacctcg gataatgtct tcatggcgat cgatattttc
       61 gcaaagaacg ttattgatcc ggagacagga gcactcggca caacgggaac ggttggggca
      121 acgctgtgca ccacctgcgc tcctcccccg ggcgaaacgc caattccggg cgcactgcct
      181 ctcttcacga gcgggatggg tgtgcttggt ttcctcggtt ggcgtcggaa gcgccggatg
      241 gcacggcaag ctgcctaaac gcgaactttg cgcagagatg caggcggccc ttcggggccg
      301 ctttgcgttt caaaaactac ttttcagcga tcgcacgagc acggcgcatg aaccagggcg
      361 cgccggcatc aaggtgatca cactgcgtta actacggaac atgcaggacg caggacagtt
      421 cctgctcggc gatcatcagc gatagtccca gaaaacccgc agatttacca ggattcatcg
      481 aattgttcgg taggccgcca tcatcgcgct atgatggcgg cagagcaacc aatcgagtac
      541 aaattgcgat tgccgaaagg gcttttgaga tgcatctcga gcgacgcatt gcggtaaccg
      601 gcggagccgg ttttttggga tcgcatcttt gcgaacggct tctgtccgag ggcgccgagg
      661 tcatttgcat tgacaatttt ttcaccggcc cgcggcggaa tatcgaacat cttctggatc
      721 acaaacgctt cgagctcatt cgccacgacg tgacgttccc gatctatctg gagatcgacg
      781 aaatctacaa tctggcgtgc ccggcatctc ccattcacta ccagcatgat ccggtgcaga
      841 cgaccaagac gagcgtccat ggggccatca acatgcttgg cctggccaag cgggtcagag
      901 caaagattct tcaggcgtcg acgtcggaag tgtacggcga ccccaacgtg catccgcaaa
      961 cggaggagta ctggggacat gtcaatccga tcgggccgcg ctcgtgctac gacgagggaa
     1021 agcgttgcgc cgagacactg ttcttcgact actggcggca acacaaactg agaatcaagg
     1081 ttgcccgcat attcaacacg tacggccctc gcatgcatcc taacgacggg cgcgtggttt
     1141 caaatttcat cattcaggcg ttgctcggcc gcgacattac gatttacgga gacggctcgc
     1201 agacccgttc gttttgctac gtcgacgacc ttatcgacgg gctggttcgt ttgatgaatt
     1261 cccccgatca ggtcaccggg ccgatcaatc tcggcaatcc gattgaattc tcgatgctgc
     1321 aactggcaga ccaagtgctg gcgttgacgg gctcacgctc gcgagttgtg catcggccat
     1381 tgccgcaaga cgacccacgc cagcggcagc ctgatatttc gaaggcgcag gaatcgctcg
     1441 gctggcgtcc ggccgttcct ctcaaggacg gattggtcaa gacgatcgaa tattttgaga
     1501 ctctgcttcg cgagcagggg acgctgcagc tcattaccga gacgcgtccc aaacacgtgg
     1561 gtgctgttgc gcgctagacg ggcgggttcc cgcggaaagc ggcgacgtcc ccacaaaata
     1621 gttctgttga cgaatcagcg agtgtggaag cttcggtgtc gctatggaga cacctgatgc
     1681 aaccagaact gatggcagtc attgtgttgt ggcttgcgtt acaggttccg cttggaagct
     1741 ttctgggtga atgcattcgt ttgggagccg gccgataccc gcttccctga ataagccgat
     1801 cgcgttccat ccatccggaa agttcgactg atgtccggcc aagcagccag ccggcggtgg
     1861 gagtgcgcct acagcatcga ccagaccagg gcgcagaggc cgtacgccaa tgctgacacc
     1921 caggccatcg tcagtgccag tccgacaact atactcgccg acgacaacgg ccgcgccagg
     1981 aacgaaaaac ggctgcgaac tgcgggcaaa ctcttcactg acttcatcct ctaactgtta
     2041 cgtgccggca aacagcctcg cctgagttac gagaggcagg cgaggctgcc ggccgcgctg
     2101 gggtgtggca ttgcccagcg gatctggaat tcatcgcaga gcacgaattg acctttgcaa
     2161 gtcatgcaat tctcagtccg taccgtgacg ttgcgacgac aaaacgatag cgctttcgca
     2221 gagccgctcg atgtgacgct ggtcacagat gttcaaccga aatttatctc ccgtgctatg
     2281 aatcgccgca caccacgctt taggccccca gggcaggtcc cccggaaaga agcggcagtt
     2341 caaccgaacc ggcgctgtct cgcggatgtt tggcgcaggc cgaaacggtt ttccgttgct
     2401 tccgcaaacg cgcaaaagca gtccctccgg caagtgcgcc gccgaacagc catagtgcgc
     2461 cgggaagcgg tgtttcgggc gtgtttgcgc cactgtgggc aaaggtgatt tttacgacca
     2521 tgtcgtcgcc gtcgaaatcg ggaaaaacgg tgaactttcc ggtgtcgtcg aagaaggcgt
     2581 aggcggtctg cccgtccggg gtcacgtaaa acccgagcag aacgagccaa ccgatattgc
     2641 cgccgttgac ggcaacccga tccgggtgcg cggtctggtc ggcaaacagg aaattcacca
     2701 gcgacgctcc ac
//
LOCUS       DASSRL010000420         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_571060, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000420 DASSRL010000000
VERSION     DASSRL010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571060"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1278
                     /locus_tag="VFL49_08530"
     CDS             298..1278
                     /locus_tag="VFL49_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912063.1"
                     /translation="MLLLRQIVALFAFAGAIIACAPAAIADDYPSRPVKIIVPFGAGG
                     PTDVYTRAIAEELRKSLHQPFVMENRPGAGTTIGTDVVAKAPPDGYTLLMISGTQTVN
                     ETLYAHKPYQLMRDLVPIAPLIDTDLVLVVHPSVPAKTLGELLALARAKPGTLNYGSS
                     GPGSNYHMAGELLKNLTGIDIVHVPYKGSTGARNDILSGQIQILFDSVPTMAPQIKAG
                     MVRALGTSGKARSPILPDVPTMAEAGVPGFNATLWVGFMAPAGTPKPIVDKLHDEITK
                     ILRRPDIKQAWEKTGATPIVMTQPEFKSFMEAQVAKWAEVIKTNHIAPVN"
     gene            1300..>2030
                     /locus_tag="VFL49_08535"
     CDS             1300..>2030
                     /locus_tag="VFL49_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7912064.1"
                     /translation="MNEMSKPAAAIKGEDHWTTKDGGVKLFLFEKHAGDPAKSIGTIL
                     FVHGSSMASQPTFDLQVPGRPDSSAMDHFAGRGYDCWCVDMEGYGRSTKDRDNNAPIS
                     QGADDCYAAALYIQKLRGVRPFLVYGISSGALRAAMFAERHPELVARLALDAMVWTGE
                     GSPTLEQRRKKLPEFQSKNRRPIDKAFIHSIFDRDHPGTAEEKVINAFADAVVALDTS
                     VPTGTYVDMCSKLPVVNPEKIAVPTL"
BASE COUNT          421 a          660 c          586 g          363 t
ORIGIN      
        1 gctcttccga tctgcaactc cgccttaact ggattttacg cccgtccgga ccccgcggca
       61 gccgaacggc gccggtttac cccgcacaaa caggcccttg tttgttggaa tagcctcaag
      121 aacgggagcg gccgaggctt agcgcagcat gtcaggggtg gcaagccgac attgggcggc
      181 atccacggcc gttccgcttc aatagaccgg gcgcgcgcac tctcgatttg acacggcaat
      241 aacccgccct cgatagtcgg cgccggcgac atcaattgca caacctggaa aacgaccatg
      301 ctgctcctgc gacaaatcgt ggccctgttc gcgttcgcag gcgcgatcat agcttgcgcg
      361 cccgccgcaa tagccgatga ctatccctcg cgcccggtca aaatcattgt tccctttggc
      421 gcgggcggcc caaccgacgt ctatacccgc gccattgccg aggaactgcg gaagtcgctg
      481 caccagccct ttgtcatgga aaaccgcccc ggtgccggta cgaccatcgg cactgatgtc
      541 gtcgcgaagg cgccgcctga cggctacacg ctgttgatga tttcaggcac gcagaccgtc
      601 aacgagacgc tctatgccca caagccgtat caactcatgc gcgacctcgt gccgatcgcg
      661 ccgctcatcg acaccgatct tgttctcgtc gtgcatccat ccgtgccggc aaagacgctg
      721 ggcgaacttc tcgcgctcgc gcgcgccaaa cccggcactc tcaattacgg ctcgtcaggg
      781 ccaggctcga actaccacat ggccggcgaa cttctgaaaa atctcaccgg catcgacatc
      841 gtgcatgtgc cgtacaaggg atcaaccggc gcccgcaacg atatcctcag cggccagata
      901 caaatcctgt tcgattcggt gccgaccatg gcgccccaga tcaaggccgg gatggtgcgc
      961 gcgcttggca ccagcggaaa ggcgcgttcg ccgatattgc cggatgtccc gaccatggcg
     1021 gaggccggcg tgccaggttt caacgcgacg ctatgggtgg gcttcatggc cccggccggc
     1081 acgccaaagc cgatcgtcga caagcttcat gacgagatca ccaagatcct gcgtcggccc
     1141 gacatcaagc aagcatggga gaagacgggc gcgacgccga tcgtaatgac gcagcccgag
     1201 ttcaagagct tcatggaggc ccaggtcgcg aaatgggcgg aggttatcaa gaccaaccac
     1261 atcgcaccgg tcaactgaaa ttgtccaaca ggtgatgtga tgaacgagat gagcaagccg
     1321 gccgccgcga tcaaaggcga ggaccattgg acgaccaaag acggcggcgt gaagctgttt
     1381 ctgtttgaaa agcacgccgg cgatccggcc aagagcatcg gcacgatttt gttcgtgcac
     1441 ggctcgtcga tggcgtcgca acccacgttc gacctgcagg tgccgggccg gcccgattca
     1501 tctgcgatgg atcacttcgc ggggcgcggc tacgactgct ggtgcgtcga catggaaggc
     1561 tatggccgct cgaccaagga tcgcgacaat aatgctccca tttcacaggg tgccgacgac
     1621 tgctacgccg cggcactcta catccagaaa ctacgcggcg tgcggccatt cctggtttac
     1681 ggaatttcgt ccggcgcgct gcgcgcggcc atgttcgccg aacgtcaccc ggagctggtt
     1741 gcgcgacttg cgctcgacgc catggtgtgg accggcgaag gttcccccac gcttgagcag
     1801 cggcgaaaga agctccctga attccagtcg aagaaccgca ggccaatcga caaggccttc
     1861 attcattcga tcttcgaccg cgatcatccc ggcaccgccg aggaaaaggt catcaatgct
     1921 tttgccgacg ccgtggttgc gctcgataca tcggtaccga ccggcaccta tgtcgacatg
     1981 tgctcgaaac tgcccgtggt caatccggaa aagatcgccg tcccgaccct
//
LOCUS       DASSRL010000421         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1725848, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000421 DASSRL010000000
VERSION     DASSRL010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725848"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFL49_08540"
     CDS             complement(<1..374)
                     /locus_tag="VFL49_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7912065.1"
                     /translation="MPAPAKSKTAKPAKVSPAKSAAASPAARPVKKGDHVFLVDGSGY
                     IFRAYHAIRFEPRTPDGTHVNAVYGFCNMLWKLLKEMKPDERPTHLAVVFDKSEKTFR
                     TEMYKDYKAHRPPPPDDLIPQFP"
     gene            complement(434..1468)
                     /locus_tag="VFL49_08545"
     CDS             complement(434..1468)
                     /locus_tag="VFL49_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET7912066.1"
                     /translation="MNGTPIHHGRIDWVDYAKGFCIVFVVMMHSTLGVEAVVGHEGWL
                     HAVVAFALPFRMPDFFLISGLFLANVIDRPWRQYVDRKVVHFAYFYLLWMTIQFAVKA
                     PGMIHEQGAIEVARLFLLSFIDPFGTLWFIYLLPVFFVFIKLTRRVPVPVIWLFGAAL
                     EIAHINTGWMVPDEFAARFVYCYTGYIFARPIFALTSRAQAAPLLAFGGLLAWGLLNG
                     TMVHLGYERLPFVSLALGLAGACAVVTAAALMAKSDLFKPLRYCGRNSIVIYLAFFLP
                     MAATRAVLLKSGLIADVGTISALVTLAGVIGSLCLFWAVRGTPACFLFERPSPFWLAP
                     PKRVVLQPAE"
     gene            1604..2914
                     /locus_tag="VFL49_08550"
     CDS             1604..2914
                     /locus_tag="VFL49_08550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET7912067.1"
                     /translation="MRVAMIGTGYVGLVSGACFADFGHEVVCVDKDKTKIAALLRGEM
                     PIYEPGLNDMVAVNARAGRLKFTTELGGAVADADAVFIAVGTPSRRGDGFADLSFVYD
                     AAREIARALDGFTVVVTKSTVPVGTGDEVERIIRETRPDADCAIVSNPEFLREGAAIR
                     DFKLPDRIVIGTTDARAREVMTGLYRPLYLNRSPIVFTGRRTAELIKYAANAFLATKI
                     TFINEIADLAEKVGADVQEVARGIGLDNRIGSKFLHAGPGYGGSCFPKDTVALIKTAQ
                     DHASPLRIVEAVAAVNDARKRAMARKVSGALGGDVRGKTVAILGLTFKPNTDDMREAP
                     SIPLITALQDMGAKVRAYDPVGMEQAKSLIGNVTFCDDAYACAKSAAALVIVTEWEQF
                     RALDFDRLKSVMAQPVLVDLRNVYRPEDIVRHGFVYEGVGRARD"
     gene            3056..>3338
                     /locus_tag="VFL49_08555"
     CDS             3056..>3338
                     /locus_tag="VFL49_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET7912068.1"
                     /translation="MARQFDASLPIDEALPALARALSDNNAAVLVAPPGAGKTTRVPL
                     ALLEESWAKDRKILVLEPRRLAARAAAARMAATLGEQVGDTVGLRVRFGS"
BASE COUNT          674 a          997 c         1048 g          619 t
ORIGIN      
        1 ggaaactgcg ggatcaggtc atccggcggc ggcggacggt gcgccttgta gtccttgtac
       61 atttcggtgc gaaacgtctt ctccgatttg tcgaagacga ccgcgagatg cgtcggccgc
      121 tcatccggtt tcatttcctt cagcagcttc cacaacatgt tgcagaaacc atagaccgca
      181 ttcacatgcg tgccgtcagg cgtgcgcggc tcgaaccgga tcgcatgata ggcgcggaaa
      241 atgtagccgg agccgtcgac gagaaagacg tgatcgcctt ttttcaccgg gcgtgcggca
      301 ggcgatgccg cggcggattt tgcaggcgaa actttggcgg gcttggcagt tttcgatttt
      361 gcaggggcgg gcatggcccg aatgtgggcc gttggcgcgc ctttatcaat ccgccccgag
      421 ggcggttgtg gacttattca gccggctgca gcacgacgcg tttcggcggc gccagccaga
      481 acggcgaggg gcgctcgaac aggaaacacg ccggggttcc gcgcactgcc cagaacaggc
      541 agagcgagcc aatgacgcca gccagggtga cgagcgcgga gatcgtgccg acatcggcaa
      601 tcaggccgct cttgagcagc acggcgcgcg ttgccgccat cggcaggaag aaagcgaggt
      661 agatgacgat cgagttgcgc ccgcagtagc gcagcggctt gaacagatca ctcttcgcca
      721 tgagcgccgc ggcggtcacg accgcgcagg cgcctgcgag cccgagcgcc agtgaaacga
      781 acggcaagcg ctcatagccg agatggacca tcgtgccgtt gagcaggccc cacgccagca
      841 atccaccgaa tgcgagcaac ggtgcggctt gcgcacgtga cgtgagcgca aaaatcggcc
      901 tcgcgaagat gtagcccgta tagcaataga cgaaccgcgc tgcgaactcg tccggtacca
      961 tccaaccggt attgatgtgc gcgatctcga gtgcggctcc gaacagccag atgaccggca
     1021 ctggcacgcg gcgcgtcaat ttgatgaaga cgaaaaatac cggcagcaga tagatgaacc
     1081 acaaggttcc aaaaggatcg atgaacgaca ggaggaaaag acgcgcaact tcgattgcgc
     1141 cctgctcgtg aatcattccg ggcgccttca ccgcaaactg gatcgtcatc cagagcagat
     1201 agaaatacgc gaaatgcacg accttgcggt cgacatattg ccgccacggt cggtcgatga
     1261 cgttggcgag aaacaacccg gagatcagga agaaatcggg cattcgaaac ggcagtgcga
     1321 aggcgacgac ggcgtgcagc cagccctcat ggccaaccac ggcctcgaca cccagcgtcg
     1381 aatgcatcat cacgacgaag acgatgcaga agcccttggc gtaatcgacc cagtcaattc
     1441 gcccatggtg gattggagtg ccgttcatga tatgcgtgcc acaagccgcc gtatcgtttt
     1501 aatcttacga agctcaagtt cgatcgcggc tctcgcctct cgataccggc tctgctattg
     1561 aggcgccacc gacttcgacc ataaaggtta agtggattag taaatgcgcg tcgcaatgat
     1621 cggcactggc tatgtcggcc tggtttcagg cgcgtgcttt gctgacttcg gtcacgaagt
     1681 tgtttgcgtc gacaaggaca agacaaagat cgctgcgctt ttgcgtggcg agatgccgat
     1741 ctacgagccg ggactgaacg acatggttgc ggttaacgcg cgcgccggcc gtctcaaatt
     1801 cacgacggaa ctcggcggcg cggtcgccga tgcggatgca gtgtttattg cggtcggcac
     1861 gccgtcacgc cgcggtgacg ggtttgccga cctcagcttt gtctacgatg ccgcgcgcga
     1921 gatcgcgcgg gcactcgatg gatttaccgt cgttgtcaca aagtcgaccg ttccggtcgg
     1981 aaccggagat gaggtcgagc gtattatccg tgaaacccgc ccggacgccg attgcgccat
     2041 cgtgtctaat cccgaatttc tccgcgaggg agccgcgatc cgggatttca agcttcccga
     2101 tcgtatcgtc attggcacga cggatgcgcg tgcgcgagaa gttatgaccg gcttgtaccg
     2161 gcccctctat ctcaaccgct cgccgattgt gtttaccggg cgccgcacgg ccgaattgat
     2221 caaatatgcc gccaacgcat ttcttgccac caagatcact ttcatcaacg agattgccga
     2281 ccttgccgaa aaggtcggcg cggacgtgca ggaggtcgca cgcggcattg ggctcgataa
     2341 ccggatcggg tcgaagttcc tgcacgcggg accgggctac ggtggatcct gctttcccaa
     2401 ggacacggtc gccttgatca agacggcgca ggaccatgcg tcgcccctgc gcatcgtcga
     2461 ggcggttgcc gccgtgaacg atgcgcgcaa gcgcgcgatg gctcgcaagg tttccggcgc
     2521 gctcggcggg gatgtgcgcg gaaagaccgt tgccattctc ggactgactt tcaagccgaa
     2581 caccgacgac atgcgcgagg caccgtcgat tccgctcatt acggcattgc aggacatggg
     2641 cgccaaggtg cgcgcgtacg accctgtcgg catggagcag gccaagagcc tgatcggcaa
     2701 tgtcacgttc tgcgatgacg cctatgcctg cgcgaaaagc gccgctgcgc tcgtcattgt
     2761 cactgaatgg gagcaattcc gcgcgcttga tttcgaccgg ctgaagagcg tgatggcgca
     2821 gcccgtgctt gtcgacctgc gcaacgtcta ccggccggaa gacattgtcc ggcacggttt
     2881 cgtctatgaa ggcgtgggac gggcccggga ctagcgatgt ggacgggtcc gtcaatcaat
     2941 agaagccgcc gtcccccgcg cgagcggggg gcacgctaac acctcgtttt cgcatctttc
     3001 ccgatgtttg tgttagtgca ccacccgctt gtgcgggagg tgatggacaa tcgctgtggc
     3061 acgacaattc gacgcatctc ttccgatcga cgaggcactc ccggcgctcg ccagggccct
     3121 ctccgacaac aatgctgccg tgcttgtcgc tccgcccggt gccggcaaga cgacgcgcgt
     3181 gcctctggct ctgctcgaag agtcatgggc gaaagacaga aaaatcctgg ttcttgagcc
     3241 gcggcggctc gcggcgcgcg cagccgccgc ccgcatggcg gcgacgctcg gtgagcaggt
     3301 cggcgacacg gttggcctgc gcgttcggtt cggctccc
//
LOCUS       DASSRL010000422         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1673198, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000422 DASSRL010000000
VERSION     DASSRL010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673198"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFL49_08560"
     CDS             complement(<1..266)
                     /locus_tag="VFL49_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912069.1"
                     /translation="MPDLLYEEQSFGVFVLVTIILGGGAATLAGRAVAATWRPWWQVV
                     AYGFILAGAVRFIHFSLYGGTFLSLHYYLVDAAFCLAFGFLGFRA"
     gene            complement(290..1015)
                     /locus_tag="VFL49_08565"
     CDS             complement(290..1015)
                     /locus_tag="VFL49_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912070.1"
                     /translation="MTAPLLTVRGVKTFYGRVMALKGIDLEVDEGEIVTLIGANGAGK
                     STLMMTICGNPRASEGTIDFAGHDITNLPTHEIARLKLAQAPEGRRIFSRMTVFENLQ
                     MGAIVGERTEFNRDLERVLTLFPRLHQRIAQRGGTLSGGEQQMLAIARALMARPRLLL
                     LDEPSLGLAPLVVRQIFDAIKALNVESGLTVFLVEQNAYHALKLAHRGYVMVNGLITL
                     SGGGRDLLEHPEIKAAYLEGGRH"
     gene            complement(1012..1920)
                     /locus_tag="VFL49_08570"
     CDS             complement(1012..1920)
                     /locus_tag="VFL49_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912071.1"
                     /translation="MASAHGTMLQVEHLTMRFGGLVAVDDLSFSAREGQITALIGPNG
                     AGKTTVFNCITGFYKPTEGRLALRQGEAAAWEWLDTLTAGGKRSITRGANGLYLLERM
                     PDYQVAERARIARTFQNIRLFPGMTALENLLVAQHNRLMLASGYTLLGVLGFPSYAAA
                     ERAAVERAREWLDRIGLTARADDAAGNLPYGAQRRLEIARAMCTEPVLLCLDEPAAGL
                     NPRESAHLNELLLTIRKEKGISILLIEHDMSVVMEISDHIIVLDYGVKIADGSPAEIR
                     DDPKVIAAYLGVVDKDVAGVEAEVGL"
     gene            complement(1920..>2446)
                     /locus_tag="VFL49_08575"
     CDS             complement(1920..>2446)
                     /locus_tag="VFL49_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912072.1"
                     /translation="LALVTAFVTLRLRRLPVGRAWEALREDEIACRSLGINTTNTKLT
                     AFAMGAMFAGFAGSFFAARQGFISPDSFTFLESATILSMVVLGGMGSQVGVAIAAVVM
                     IGGTEIMRDLDFLKLVFGPTFDPTQYRMLLFGFAMVLIMIWRPRGLISTREPSLVLKE
                     KKIITADLIKEGRG"
BASE COUNT          523 a          727 c          756 g          440 t
ORIGIN      
        1 gcccggaagc ccaaaaatcc gaaggccagg cagaaagcgg cgtccaccaa ataatagtgg
       61 agagacaaaa aggtgccgcc atagaggctg aaatggatga accgcacggc gccggccaga
      121 atgaagccgt atgcgacaac ctgccaccac ggccgccagg tcgctgcaac cgcgcggccg
      181 gcgagggtcg ccgcgccgcc accgagaatg atggtgacca gcacgaatac gccgaaggat
      241 tgctcctcat aaaggaggtc agggatcgaa agggtcgaga ataactcgct caatgccggc
      301 ctccttcgag ataggccgcc tttatttcgg gatgctcaag aagatcgcgt ccgccccccg
      361 acagggtgat caagccattg accatgacat agccgcggtg cgcgagtttg agcgcgtgat
      421 aagcgttctg ctcgaccaag aagacggtca ggccactttc cacattcaat gctttgatgg
      481 catcgaatat ctggcgcacg accagtggcg caaggccaag cgacggttca tcaaggagca
      541 acaggcgggg acgcgccatc agcgcgcgtg cgatcgccag catttgctgc tcgccgcctg
      601 agagtgtgcc gccacgttga gcgatgcgct ggtgcagcct gggaaaaagc gtgagcacgc
      661 gttcgaggtc gcgattgaat tccgtgcgct ccccaacgat agcgcccatc tgcaagttct
      721 cgaacacagt catgcgtgaa aagatgcggc ggccttcagg tgcctgcgcg agcttgagac
      781 gcgcaatctc gtgggttggg aggttcgtta tgtcgtggcc ggcgaagtcg atcgtgcctt
      841 cgcttgcgcg cggattaccg caaatggtca tcatcaaggt ggattttccg gcgccgttcg
      901 caccgataag cgtgacaatc tcaccttcgt cgacctcaag atcgatgccc ttgagcgcca
      961 taacgcggcc gtaaaaggtc ttcactccgc gcacggtcaa aagtggtgcc gtcatagtcc
     1021 gacctcggcc tcaaccccgg cgacatcctt gtcgacgaca ccgaggtagg cggcgataac
     1081 tttcggatca tcgcgaattt ccgcagggct cccgtcggca atcttgaccc catagtcgag
     1141 cacgatgatg tggtcggata tttccatgac caccgacatg tcgtgctcga tgagcaggat
     1201 cgatatgccc ttttctttcc ggatcgtaag aagcaactcg ttgagatgag cgctttcgcg
     1261 cgggttgagg ccggcggctg gttcatcaag gcacagcagg acgggctccg tgcacatcgc
     1321 gcgggcgatc tcgagccggc gctgcgcgcc atagggaagg tttccggcgg cgtcatcggc
     1381 gcgtgccgtc agtccgatcc gatcgagcca ctcgcgcgcg cgctcgaccg cggcacgctc
     1441 ggcggcggca taagaaggaa agccgagcac accaagaagc gtgtaaccgg aggcaagcat
     1501 caggcgattg tgctgggcga ccagaaggtt ttcgagtgcg gtcatgcccg ggaaaaggcg
     1561 gatattctga aatgtgcggg cgatccgcgc ccgttcggcg acttggtaat ccggcatccg
     1621 ctcgagcaaa tagaggccgt ttgcgccgcg cgttatgctg cgcttcccgc ccgccgtgag
     1681 ggtatcgagc cattcccagg cggcggcttc gccttgacgt agagcgagcc ggccttcggt
     1741 cggcttgtag aagccggtga tacaattgaa tacggtcgtc ttgccggccc cattcggccc
     1801 gattaacgcg gtaatttggc cttcgcgcgc gctgaacgac agatcgtcga cagccacaag
     1861 tccgccgaaa cgcatcgtca gatgctcgac ttgcagcatc gtgccatgcg cagacgccat
     1921 cagccgcgtc cttctttgat cagatcggcg gtgatgatct ttttctcttt gagaaccagc
     1981 gacggctctc gggtcgagat gaggccgcgt ggccgccaga tcatgatcag caccatggcg
     2041 aaaccgaaca gcagcatgcg gtactgggtc ggatcaaagg tcggaccgaa gacaagcttc
     2101 aaaaaatcga ggtcgcgcat gatctcggtg ccgccaatca ttacgaccgc cgcaattgcg
     2161 acgccgacct ggcttcccat gccgccgagg acgaccatcg acaggatcgt ggcggactcc
     2221 aggaaggtga atgaatcggg cgaaatgaaa ccctgtcttg cggcgaagaa tgagccggca
     2281 aagcccgcaa acatcgctcc catggcaaaa gccgtgagtt tcgtgttcgt cgtgttgatg
     2341 ccgagcgagc ggcaggcgat ctcgtcctcg cggagcgcct cccaggcgcg gccgaccggc
     2401 aggcgtcgca ggcgcagggt cacgaaagcc gtcaccaagg ccagac
//
LOCUS       DASSRL010000423         7126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_658686, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000423 DASSRL010000000
VERSION     DASSRL010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658686"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFL49_08580"
     CDS             <1..593
                     /locus_tag="VFL49_08580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HET7912073.1"
                     /translation="QAAKDGAQAGGVKARRRSRSQRLIRVWRGNGDTRITIRGMNMLL
                     VKTYLDKSAIHGLGVFAGEVIRKGTKVWRFVDGFDRAYSPTQFAKLPKPARDYIWNYG
                     YRVDGEILLTSDHDHHMNHSDNANTHWHNGHIVATRTIRKGEEITNNYRQFDAGFCAA
                     FLKRKLRVVANNPVPREDATWLRRASRLRKVDRKVV"
     gene            735..1988
                     /gene="tyrS"
                     /locus_tag="VFL49_08585"
     CDS             735..1988
                     /gene="tyrS"
                     /locus_tag="VFL49_08585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7912074.1"
                     /translation="MSAYKSDFLNVLAERGFIHQVSEPETLDARAKAGAITAYIGFDC
                     TAGSLHVGSLLPIMMLHWLQQTGHRPIALMGGGTTRVGDPSGKDESRRILTDEMINEN
                     LAGIRAIFSKFLKFEGAGGNAVMANNADWLNTLNYIDFLRDVGRHFSVNRMLSFDSVK
                     LRLDRQQELSFLEFNYMVLQAYDFVELNRRHGCILQMGGSDQWGNIVNGIDLGRRMLN
                     VQLFALTAPLITTSSGAKMGKTAAGAVWLNAELVRPYDYWQYWRNTEDGDVVRFLKLF
                     TVLPIREIARLAALKDNEINDAKKILATEATALVHGRAAADQAAETARKTFEEGATSL
                     SLPTVPVSRKDLSAGIGVLNAFVLAGLAASNGEVRRAISNGAVAINDVRITSDKHVIA
                     SKDMTSDGVIKLSLGKKQHVLLKPH"
     gene            complement(1989..5540)
                     /locus_tag="VFL49_08590"
     CDS             complement(1989..5540)
                     /locus_tag="VFL49_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912075.1"
                     /translation="MVERVPYRHRAAQRLVRRRWTDLRYAVSDMFRRRLVRRLLLGAA
                     ICVSLAIIGVLGLWWRLSSGPIELDLATPWLKAAIEENFGGNHTVAVGGTQLERDEKG
                     RTSLRIRDIVVRDADGTIVASAPKAEVGLSGRSLFVGQLRAQSLNLVGAEMAVRIETD
                     GRLTVFAGADKRPIATATANGNIPPVDQQAPSVNPQGVRAEFEKLAGIMAWIDALGQT
                     GLDGHDLRELGLKNGNLVVDDRRNGKRWTFDGINASLTRPRRGGVIFRLESDNPERPW
                     VLSAAMRPLTDGVRAVGIEARKVSMHDILLALRINSEDFDIDLPVSASIRADVLADGT
                     PQVVQGQLFTEEGTVFDRNNDRVSFPVDRADVRFNWDSRRRSLVIPFQVQSGGNQFTM
                     RATLDAPHEQNGVWLINLTRGDSVIDPVILAPGTSSDDEGVALNRVSMRLKVDTNRKR
                     IDLEQGDLSRIDTRPSQNIGLALTGRLDYGGKEPHLAFGVAGTRMPVYALKRIWPIFA
                     AADVRSWVETHISGGMVERVVVAGNAAVANFRHDGPPTPDDGLSVDIETSGTTLRPIA
                     SLPAIRDADLTVRIRGRAATVNVGRGTVEVAPGRRLNIAGGVFEVPDTHPKPAAARAN
                     FRIDGSVPAAVVLLSSEGLRDIVGMSLDPASTRGTITAQVAVKLALSRTMSDDSSSYT
                     INADLTNFAADKMLLGQRIEASTLKVTASGDGYQIKGDAKVNGTPASIDLRKQKGDAD
                     AELRMQSVIDDAARRRLGMDVGNAIIGAVPVKVVGKINDDGKDEGLNVEADLTPVKID
                     NLFPGWIKAAGRPAKATYKMLRNAKSVRIDDLSIDGSGATIRGSIELDGSTNEIVTAN
                     FPVFALSDGDRVALKADRAADGVLHVSMRGDIYDGRHFVKSSLAGSSADKSKPKQVDL
                     DLEVKVGTVAGHNGETLRGLDLKMSRRGGHIRSFVMTSKIGRDTPLNGDLRIRSRDNH
                     QVIYFETDDAGALFRFTDMYPRMYGGQMWLAMDPPTQEQSPQIGTLYIRRFVVRGEPT
                     LDKIVSGAPNSHGESGVDFSEMRAEFTRYPGKMAVRDGIVRGPLVGATVEGNIDYVRD
                     DVHLRGTFVPFYGLNNMFGQIPIVGLFLGGGNKEGLVGINYEAVGPPGAPRITVNPVS
                     AMAPGLLRKFVPSPGTFDPKFSPAPGAYDQNFIPPSR"
     gene            5751..6221
                     /locus_tag="VFL49_08595"
     CDS             5751..6221
                     /locus_tag="VFL49_08595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7912076.1"
                     /translation="MDRELIAGAKAPAFTLRRDGGGKVSLKDFKGGKLVLYFYPKADT
                     PGCTKEAIEFSRLRADFAKAGTAILGVSADPVAAQDKFISKHRLTIALGSDEAKTMLE
                     AYGAWGEKSMYGRKFMGVLRKTFLIGADGRIIRIWPKVRVPGHARDVLLAARTA"
     gene            6422..>7126
                     /locus_tag="VFL49_08600"
     CDS             6422..>7126
                     /locus_tag="VFL49_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HET7912077.1"
                     /translation="MSRHLQRSHGGYTLTHAGHQIRIGPVAFWIVVGALVIMGVWSIA
                     TGSYFAFRENVLTRLIGRQAEMQFAYEDRIAELRAQVDRIMSRQLLDQEQFEQKLNSL
                     LQRQAILEQRTTALGGETLTTGSVKPSRVAPPGAAERIPRPSPISDTVIFTAPPDREA
                     RLYSRELPAAATQIAERSTSGGLTGVLARISTSLDKVEQKQAATLSTLEERIDTKARR
                     MRGVLADLGVEPVKSRG"
BASE COUNT         1413 a         2252 c         2134 g         1327 t
ORIGIN      
        1 gacaagcggc taaagacgga gcgcaagccg gcggcgtaaa agcccggcgg aggtccaggt
       61 cgcaacgctt gattcgtgtc tggcggggaa atggcgacac gcggatcacc atccgcggga
      121 tgaacatgct tcttgtcaaa acttacctcg ataaaagcgc catccacggc cttggcgttt
      181 ttgccggaga agtcattcgc aaggggacaa aggtctggcg attcgtcgac ggcttcgaca
      241 gggcctactc gccgacccag ttcgcaaaat tacccaaacc cgcgcgcgac tacatctgga
      301 actacggata ccgggtggac ggagaaattc tgctcaccag cgatcacgac caccacatga
      361 accactccga caatgccaac acccattggc acaatggtca catcgttgcg acgcgtacca
      421 ttcgcaaggg cgaagaaatt accaacaact atcggcagtt cgatgcgggt ttttgcgcgg
      481 ccttcctgaa gagaaaatta cgagtcgtcg ctaacaaccc agtgccgcgc gaggacgcca
      541 cctggctgcg gcgggccagt cgtcttcgca aagttgatcg gaaggtcgtt taagctacac
      601 atgcgcgaag gtccgcgcct cgacctgccg ccgacgcggg ccgcgcttgc ccgccgtcat
      661 gtattgttga atccgacacc ggacctgtta caccaccctt cgtatgcgac gagaccacgg
      721 cacgagagtc cagaatgagt gcgtacaaat ccgattttct caacgtcctg gccgaacgcg
      781 gcttcattca ccaggtctct gagcccgaga cgctggatgc gcgcgcgaaa gccggcgcga
      841 tcaccgccta catcggattc gattgtaccg ccggctcgct gcacgtcggc tcgctgctgc
      901 cgatcatgat gctccactgg ctccagcaga ccggacacag gccgatcgcc ctgatgggcg
      961 gcggcacgac acgcgtcggc gacccctccg gaaaagacga aagccgccgg atcctcaccg
     1021 acgagatgat caacgaaaac ctcgctggca tccgggctat cttttccaaa ttcctgaaat
     1081 tcgaaggggc cggcggcaac gccgtcatgg ccaacaatgc cgactggctg aatacgctca
     1141 attacattga tttcctgcgc gacgtgggcc ggcacttttc cgtcaaccgc atgctctcct
     1201 ttgattccgt caagttgcgc ctcgatcgcc agcaggaatt gtcatttctc gaattcaatt
     1261 acatggtttt gcaagcctac gacttcgttg agctgaatag gcgtcatggc tgcatcctgc
     1321 agatgggcgg ctccgatcaa tggggcaata tcgtcaacgg catcgatctc ggccgccgca
     1381 tgctcaacgt gcaattgttc gcgctgaccg cgccgctgat cacgacgtcg tccggcgcca
     1441 agatgggcaa gaccgcggca ggcgcggtgt ggctcaatgc cgaactggtc aggccgtatg
     1501 attattggca gtactggcgc aacaccgagg acggcgacgt cgtccgtttc ctgaagctct
     1561 tcacggttct gccaataagg gaaatcgcgc gtcttgccgc gctcaaagac aacgagatca
     1621 acgacgcgaa gaagattctc gcgaccgagg caaccgcgct ggtgcatggc cgcgctgccg
     1681 cggatcaggc cgccgaaacg gcgcgaaaga ccttcgaaga aggcgccaca tcgctgtcgc
     1741 tgccgaccgt tccggtgtca cgaaaggatt tgtcggctgg catcggcgtg ctcaacgctt
     1801 ttgtgttggc cggccttgca gcgtcgaacg gcgaagtccg gcgcgccatc agcaacggtg
     1861 cggttgccat caacgatgtc cgcattacga gcgacaagca cgtgatcgcc agcaaggaca
     1921 tgacctcgga tggcgtaatc aagctgtcgc ttggcaaaaa acagcatgtg ctcctgaagc
     1981 cgcattaatc agcgcgaagg cggaataaag ttctgatcgt acgctccggg cgccggagaa
     2041 aacttcggat cgaatgtgcc cggcgacggg acaaatttcc gcagcaggcc gggcgccatc
     2101 gcgctgaccg gattgaccgt gatcctcggc gccccgggcg gcccgacggc ctcgtagttg
     2161 atgccgacca agccttcttt gttgcccccg ccgaggaaca ggccgacgat cggaatttgc
     2221 ccgaacatat tgttgagccc gtagaacggc acgaacgtac cccgcaggtg aacgtcgtcc
     2281 ctcacgtagt cgatatttcc ttcgacggtg gcgcccacca gagggccgcg cacgataccg
     2341 tcgcgaacag ccatcttgcc gggatagcgc gtgaattcgg cgcgcatctc actgaagtcg
     2401 accccggact caccgtgcga attcggggca ccggacacga tcttgtcgag cgtcggctcg
     2461 ccgcgcacga cgaacctgcg gatgtagaga gtaccgatct gcggtgactg ttcctgggtc
     2521 ggcgggtcca tcgccagcca catctggccg ccgtacatgc gcggatacat gtcggtgaac
     2581 cggaacagcg cgcctgcatc gtccgtttcg aaatagatga cctggtgatt gtcgcgcgaa
     2641 cggatgcgca gatcgccgtt gagaggcgtg tcacggccga tcttgctggt catgacgaag
     2701 ctgcggatgt ggccaccgcg gcgcgacatc ttcaaatcca ggccgcgcaa tgtttcgccg
     2761 ttgtggcccg cgaccgtgcc tacttttacc tcgaggtcga gatccacttg ctttggcttg
     2821 cttttgtcgg ccgacgagcc agccagcgac gatttcacaa aatgccgtcc gtcataaatg
     2881 tcgccgcgca tggagacgtg cagtacgccg tcggcggcgc ggtccgcctt gagcgcgacc
     2941 ctgtcgccat cggacaaggc gaagaccgga aaattcgcgg taacgatttc gttggtcgaa
     3001 ccgtccagct caatcgagcc gcggatcgtg gccccggaac cgtcgatgct caaatcgtca
     3061 atgcggacgc tcttggcgtt cctgagcatc ttgtaggtgg cctttgcggg acgcccggcc
     3121 gcctttatcc agccgggaaa gaggttgtcg atttttaccg gggtgagatc ggcttcgaca
     3181 ttcaagccct cgtctttgcc gtcatcattg atcttgccga cgaccttgac cgggacagcg
     3241 ccgatgatcg cattgccgac atccatgcca agccgccggc gagccgcatc gtcaatcacc
     3301 gactgcatgc gcaattcggc gtccgcatct cccttctgct tgcgcaaatc tatcgatgcc
     3361 ggcgtgccat tcaccttggc atcacctttg atctgatacc cgtcccccga ggctgtgacc
     3421 ttgagagtcg aggcctcgat cctctgaccg agcaacatct tgtcggctgc gaagttcgta
     3481 agatcggcgt tgatcgtgta cgaagacgag tcgtccgaca ttgttcggga gagcgccagc
     3541 ttcaccgcaa cctgcgccgt aatggtgccg cgggtggacg ctggatcgag cgacattccg
     3601 acaatgtcgc gcagaccctc gctggagagc agcaccactg ccgccggcac ggaaccgtcg
     3661 atccggaaat tggcgcgcgc agccgcgggt ttcggatgcg tgtcgggcac ttcgaaaaca
     3721 ccgccggcaa tattgagcct gcgcccggga gcgacctcaa ccgtgccgcg cccgacattg
     3781 accgttgccg cgcggcccct gatccgcacg gtcaggtcgg catcgcgaat ggccggcagg
     3841 ctggcgatcg gacgcaacgt tgtgccgctc gtctcgatgt cgaccgagag accgtcgtcg
     3901 ggcgttggcg ggccgtcgtg cctgaagttg gccaccgccg cattgcccgc aacgacaacg
     3961 cgctcgacca tgccgcccga aatgtgcgtc tcgacccagg agcgcacgtc agcagcggca
     4021 aagatcggcc aaattcgctt cagcgcgtag acaggcatgc gcgtgccggc gacgccgaaa
     4081 gcaagatgcg gctccttgcc gccatagtcc aaacggcctg tgagcgcgag gccgatgttt
     4141 tgggacggac gcgtgtcaat tcggctgaga tcaccctgct ccaaatcgat ccgtttgcgg
     4201 ttcgtgtcga ccttcaggcg catggacacg cgattgaggg ctacgccctc atcatcggac
     4261 gatgtgcccg gcgcgagaat gactggatcg atgacggaat cgccccgggt caggttgatc
     4321 agccatacgc cgttttgttc atgtggcgcg tccagggttg cacgcatggt gaattgattg
     4381 ccgcctgact gaacctgaaa cggaatgaca aggctgcggc ggcggctgtc ccaattgaac
     4441 ctgacgtccg cccgatcgac agggaaactg acccgatcat tgttcctgtc gaagaccgtt
     4501 ccctcttccg tgaacagttg gccttgaaca acttgcggcg ttccgtctgc cagcacgtcg
     4561 gcccggatgc tcgccgacac gggcagatca atgtcgaaat cttcgctatt gatccgcagc
     4621 gcgagcagga tgtcgtgcat ggaaaccttg cgcgcctcga taccgaccgc acgtacacca
     4681 tcggtcaggg gacgcatcgc ggcgctcagc acccagggcc gctcggggtt gtccgattca
     4741 agccggaaga tgaccccgcc tcgccgcggc cgcgtcaggc ttgcgttgat gccgtcgaat
     4801 gtccaacgtt tgccgttgcg ccggtcgtca acgaccaggt tgccgttctt cagacccagt
     4861 tcacgcagat cgtggccgtc gagcccggtc tgaccgagcg catcgatcca cgccatgatc
     4921 ccggcgagct tttcgaactc ggcacgcacg ccttgcggat tgacggaagg agcctgctga
     4981 tcgaccggag gaatattacc gttcgcggtg gcggtggcga tcgggcgctt gtcggctccg
     5041 gcaaacacgg tcagccgccc gtccgtttcg atacggaccg ccatttccgc gccgaccaga
     5101 ttgaggctct gggctcgcag ctggccgacg aacaggcttc ggcccgaaag tccgacttcc
     5161 gctttcggcg cacttgcgac aatcgtgcca tcggcatcgc ggaccacgat gtcgcgaatc
     5221 ctcaacgacg ttcgtccctt ttcatcgcgc tcgagctggg tgccgccgac ggcaaccgtg
     5281 tgattgccgc cgaaattctc ctcgatcgcg gctttaagcc agggggtcgc caggtcgagc
     5341 tcgatcgggc cgctcgacag acgccaccac aggccgagca ccccgatgat cgccagggac
     5401 acgcagatcg ctgcgccgag aagcagacgc ctcacaaggc gcctgcgaaa catatcggag
     5461 acagcgtagc gcagatcggt ccatcgccgc cgcaccaggc gctgcgcggc acggtgacga
     5521 tacggaacgc gctccaccac gacagcatgg tgaactcgat gtcggcgctg gtgtccgccg
     5581 tctagcatgc gttgggtcgc accccttgtc tggcgcagcg cacataaagc aatgccgggc
     5641 aggcaagcga ccgctgcggc ctacggcaca tgtgtatgga acctgttgcg cccctccgac
     5701 gcaaatggca tgtgcgacat gaccgctcga aaacgacgaa aggaaggcat atggaccgcg
     5761 aacttattgc gggtgcgaag gctccggcct tcacccttcg cagggacggc ggcggcaagg
     5821 tttcattgaa agatttcaaa ggaggcaagc ttgtcctcta cttctatccc aaggccgaca
     5881 cgcccggttg cacaaaagag gcgatcgagt tctcccgcct tcgcgctgat tttgccaagg
     5941 ccggcaccgc cattctcggc gtctcggctg atccggttgc cgcgcaggac aaattcatat
     6001 ccaagcatcg cctgaccatt gcgttgggct ccgacgaggc caaaacgatg ctggaagcgt
     6061 acggagcctg gggcgagaaa tcgatgtacg ggcgcaaatt tatgggggtc ctgcggaaga
     6121 cgttcctgat cggcgcggac ggccgcatca ttcggatttg gccgaaagtc cgcgtacctg
     6181 ggcatgcccg ggacgttttg ctggctgcac gcactgcgta atcctcagta atcgctgaag
     6241 aatcccagcg atagctcatt ccgattaccg gtttcttaac catatccgat tgatatcgac
     6301 tctgccggcc agcggctgcc ttcggctgcg cggagtttcg atgtcgcatt cgtcgtcctc
     6361 ataccatccg gtttccgagc aagccggccg ccattcgcat acgcgcgcgt ccgcagcggc
     6421 gatgtcgcgc catctccagc gcagccacgg cggctacacg ctcacgcatg ccggacatca
     6481 gatccggatc ggaccggtgg cattctggat cgtcgtgggc gccttggtga tcatgggcgt
     6541 ctggtcgatc gcaaccggaa gctatttcgc atttcgggag aatgtgctca cacggctgat
     6601 cggccggcag gccgagatgc agttcgccta cgaagaccgc attgccgaac tgcgcgccca
     6661 ggtggaccgg atcatgagcc gccagctgct cgaccaggag cagttcgagc aaaagctcaa
     6721 ctcattgctg caacggcagg caatcctgga acagcgcacc accgctctgg gcggcgagac
     6781 cctaacgacc ggcagcgtca agccctcacg cgtcgcgccg cccggagcgg ccgaacggat
     6841 cccgcgcccc tcgccgatca gcgacacggt gatcttcacg gcgccgccgg atcgcgaggc
     6901 gcggctctac tcgcgcgaat tgccggcagc ggcgacccag atcgcggaac gctcgacgag
     6961 cggcgggctc accggcgtgc tcgcgcggat atcgacgtcg ctggacaaag tcgaacagaa
     7021 gcaagccgcg acgttgtcga cccttgaaga gcgcatcgat acaaaagcgc gccgcatgcg
     7081 cggcgtgctc gcggatctcg gagttgaacc tgtcaaatcg cgaggc
//
LOCUS       DASSRL010000424         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1551007, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000424 DASSRL010000000
VERSION     DASSRL010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551007"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFL49_08605"
     CDS             <1..526
                     /locus_tag="VFL49_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7912078.1"
                     /translation="GFLAAGSVLAGLPFKEIFAGHGVEGFFRESLTFAKSNSVLADMH
                     HVPLQIAMLPTAMMVIGFLIAWHFYIRRPDIPVELARQHDVLYRFLLNKWYFDEIYDA
                     IFVRPTKRLGYLLWKGGDGWLIDGFGPDGVSARVLDVTRNVVRLQTGYLYHYAFAMLI
                     GAAAFITWFMFAMH"
     gene            698..>2182
                     /locus_tag="VFL49_08610"
     CDS             698..>2182
                     /locus_tag="VFL49_08610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET7912079.1"
                     /translation="MNWPILSVVTFLPLFGAVLVMLLVRGNERLARGTARWIALWATL
                     VTFAISLVMVWRFDGSSAEFQFTEKYDWLGIASYHMGVDGISLPFVILTTALMPLCIL
                     ASWTVIQTRVKEYMIAFLVLETLMVGTFSALDLVLFYLFFEGGLIPMFLIIGVWGGPR
                     RVYASFKFFLYTLLGSVLMLLAIMAMYWEAGTTDIVTLLRHGFPLGLQKWAWVAFFAS
                     FAVKMPMWPVHTWLPDAHVEAPTAGSVILAAILLKMGGYGFLRFSLPMFPAASHDFAP
                     LIFTLSVVAIIYTSLVALMQEDMKKLIAYSSVAHMGFVTMGIFAATTQGVAGGIFQMI
                     SHGIVSGALFLCVGVIYDRMHTREIAAYGGLVNRMPLYAFVFMVFTLANVGLPGTSGF
                     VGEFLSLIGTFRTNTAVATLATLGVILSAAYALWLYRKIIFGNLRPSLEGIRDIGWRE
                     AVIFTPLVVLTILFGVAPKPVLDMSAASVAQLLENYNHAIAAAKT"
BASE COUNT          328 a          661 c          669 g          524 t
ORIGIN      
        1 cggcttcctc gccgccggat cggtgcttgc cggcctgccg ttcaaggaaa tcttcgcagg
       61 gcacggggtc gaagggttct tccgcgagtc gctcaccttc gccaagagca acagcgtgct
      121 ggcggacatg catcacgtgc cgctccagat cgccatgctg ccgacggcga tgatggtgat
      181 cggatttttg atcgcgtggc acttctatat ccgtcgcccg gatattccgg tggagctcgc
      241 gcggcagcac gacgtgttgt accgcttcct gctcaacaag tggtatttcg acgagatcta
      301 tgacgcgatt ttcgtgcgcc cgaccaagcg ccttggctat ctgctctgga agggcggcga
      361 cggctggctg atcgatggtt tcgggccgga cggcgtgtcg gcgcgcgtgc ttgacgtcac
      421 gcgcaacgtt gtgcggctgc agaccggcta cctctaccac tacgcattcg cgatgctgat
      481 cggcgccgcg gccttcatca cctggttcat gtttgcgatg cattagaccg atgtcgcgct
      541 cgtttaaaga caaagtattg accgcctgtc cccgcgaaag cggggaccca gggcggattg
      601 ttgcccgtgt ggcgattgtg tcgccggatt cccgcatagg cgtgcgaaag cacgccgtcc
      661 ttcggacggc tatgcgcggg aatgagcgga gaatgatatg aactggccga tcctctccgt
      721 cgtcaccttc ctgccgctgt tcggcgcggt gctcgtcatg ttgctggtgc gcggcaacga
      781 gcggctggcg cgcggcaccg cgcgctggat cgcgctgtgg gcgacgctgg tcacgtttgc
      841 gatctcgctg gtgatggtct ggcgcttcga tggcagctcg gccgaattcc agtttaccga
      901 gaaatacgat tggctgggca tcgcttccta tcatatgggc gtcgacggaa tttcgctgcc
      961 gttcgtcatt ctaacgacgg cgttgatgcc cctctgcatc ctcgccagct ggacggtcat
     1021 ccagacgcgc gtgaaggaat atatgatcgc cttcctggtg ctggagaccc tgatggtcgg
     1081 caccttctcc gcgctcgatc tcgtcctttt ctatctgttc tttgaaggcg gcctgattcc
     1141 gatgttcctg atcatcggcg tctggggcgg cccgcgccgc gtctatgcga gcttcaagtt
     1201 cttcctctac acgctgctcg gctcggtgct gatgctgctc gccatcatgg cgatgtactg
     1261 ggaagccgga acgaccgata tcgtgacgct gctgcgccat ggcttcccgc tcggcctgca
     1321 gaaatgggcc tgggtcgcct tctttgcgtc cttcgcggtg aagatgccga tgtggccggt
     1381 ccacacctgg ctgcccgacg cgcatgtcga ggcgccgacg gcgggatcgg tgatcctcgc
     1441 cgccatcctg ctgaaaatgg gcggttacgg tttcctccgt ttctcgctgc cgatgtttcc
     1501 cgccgcgagc catgacttcg cgccgctgat cttcacgctt tccgtcgtcg ccatcatcta
     1561 tacgtcgctc gttgcgctca tgcaggagga catgaagaag ctgattgcct actcgtcggt
     1621 tgcgcatatg ggcttcgtca caatggggat tttcgctgcg accacacaag gggtcgccgg
     1681 cggcattttc cagatgatct cgcacggcat cgtctcgggg gcgctgttcc tttgcgtcgg
     1741 cgtgatctac gaccgcatgc acacgcgcga gatcgccgcc tatggcggcc tggtcaatcg
     1801 gatgccgctc tatgcctttg tcttcatggt gttcacgctg gccaatgtcg gcttgccggg
     1861 gacgtcggga tttgtcggcg aattcctgtc gttgatcggc actttcagga ccaatacggc
     1921 ggtcgccacg ctcgcgacgc tcggcgtgat tctgtcggcg gcttatgcgc tgtggctcta
     1981 ccgcaagatt atcttcggca atctcaggcc ttcgcttgaa ggtatccgcg acatcggctg
     2041 gcgcgaggcg gtcattttta cgccgctggt cgtactgaca attctgttcg gcgttgcgcc
     2101 caagcctgtg ctcgatatgt ctgccgcgtc ggttgcgcag ctgcttgaaa actacaatca
     2161 cgctattgct gcggccaaga ct
//
LOCUS       DASSRL010000425         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1603238, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000425 DASSRL010000000
VERSION     DASSRL010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603238"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFL49_08615"
     CDS             complement(<1..329)
                     /locus_tag="VFL49_08615"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HET7912080.1"
                     /translation="MTLSPRASITAVHGYLPPDLLTNGELARMVDTSDAWITERTGIK
                     ERHILKGEGLGTSHMAAEAVRGLLQKTATKPDEVDLLICATTTPDMQFPSTANMICDM
                     VGIRNIG"
     gene            complement(385..1584)
                     /gene="lpxB"
                     /locus_tag="VFL49_08620"
     CDS             complement(385..1584)
                     /gene="lpxB"
                     /locus_tag="VFL49_08620"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET7912081.1"
                     /translation="MSPHIFLVAGEESGDRLGAALVSAIRGRTQGNAHFSCVGGAHMA
                     AEGVPSLFPLGDLAIVGFGSIPGRLSKILRRIREAADAVIAAKPDVLVIIDSPEFTHR
                     VARRVRKRASGIPIVDYVCPSVWAWRPGRARAMRAYVDHVLALLPFEPKVMKDLGGPP
                     CTFVGHPLTERLADLRPNELEARLRRANPPLVLVLPGSRSGEIRRMAPVFGRAIARVV
                     EQFGPLEAVVPAVPRLADSVRAAVATWSIPARVITDPDEKHEVFRTARAALTKSGTST
                     LELALAGVPMVAAYKVSPVEAMVGSILIHAPSAILANLVLGKNLVPEFLQSDCTAEKL
                     AAALVPLLGDTPERRRQIEAFSQLDDIMEIGKAKPSAHAAMLALEAVGSRSSAREQAA
                     RLSLPQA"
     gene            complement(1581..2453)
                     /gene="lpxI"
                     /locus_tag="VFL49_08625"
     CDS             complement(1581..2453)
                     /gene="lpxI"
                     /locus_tag="VFL49_08625"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663981.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET7912082.1"
                     /translation="MSNAVAVGQDGPLAMICGGGTLPLAVADSAAKRGRNVVLFPLRG
                     HADAALTTRYPHHWLRIGQLGAFTRLARAADCRDVVFIGALVRPSLWSLRPDLLALKS
                     LPKIIQAYRGGDNHLLTGVSKLFEEQGFRLVGAHEVAPEILVPEGALGRIDVSEQERA
                     DIAFGFDYLQASGAFDVGQAVVVAGKRILAVEAAEGTDQMLDRVAELRASGRIGDASG
                     TGVLVKAPKPDQDQRFDLPSIGPRTVTGVVGAGLAGIAVVSAKTIIADAESVVREADR
                     AGIFVVGVPSGSKT"
BASE COUNT          527 a          821 c          714 g          478 t
ORIGIN      
        1 ctgccgatgt tgcggatgcc gaccatgtcg caaatcatgt ttgcagtcga tggaaactgc
       61 atgtcgggag tcgtcgtcgc gcaaatcagg agatcgactt cgtcgggttt cgtcgcggtt
      121 ttctgcaaca agccacgcac cgcttcagcc gccatgtgcg atgtaccaag accctcgcct
      181 ttgagaatat gacgttcctt gattcccgtg cgctcggtga tccaggcgtc gctcgtgtcg
      241 accatgcgcg caagttcacc gttggtcagc aggtccggcg gcaaatagcc gtgaacagct
      301 gtgattgatg cgcggggaga aagggtcaag ggctggaatc cggtttcctg caatggcata
      361 cgcccaactg tgctgggcgg caagctaggc ttgcggcagc gataacctcg ctgcttgttc
      421 tcgcgcgctg cttctgcttc cgacagcctc aagggccaac attgcggcgt gcgcgcttgg
      481 cttcgctttt cctatttcca ttatatcgtc cagctggctg aaggcttcaa tttgcctgcg
      541 gcgctccggt gtatcgccaa gcagcggcac gagcgctgca gcgagttttt cggccgtgca
      601 atcgctttga agaaattccg ggacaaggtt tttgcctaat acgaggttgg caaggatcgc
      661 cgaaggagca tgaattagaa tacttccaac catcgcctcg accggcgata ctttgtacgc
      721 ggcaaccatg ggaacgccgg caagcgccaa ttcgagtgtc gacgttcccg attttgtcag
      781 cgcggcccgc gccgtccgga atacctcgtg cttctcgtcc ggatcggtta tcacgcgcgc
      841 cggaatggac catgtcgcaa cggctgcccg cacggaatcg gcaagtcgcg ggacagccgg
      901 cacgactgct tcaagcggac cgaattgttc aacgacacga gcgatcgccc gaccgaatac
      961 cggcgccatg cgccgaattt cgccgctacg gcttccaggc aaaaccagaa ccaagggcgg
     1021 atttgcccgg cgcaagcgcg cctcaagttc gtttggccgc agatcagcca ggcgttcggt
     1081 cagcggatga ccaacgaagg tacaaggcgg gccgcccaga tccttcatca ccttcggctc
     1141 gaatggcaga agcgccagca catgatcgac gtaagcacgc atcgcacgcg ccctgcccgg
     1201 acgccatgcc cagacggaag gacagacgta gtcgacgatc gggatacctg aagcgcgctt
     1261 gcgcacgcga cgcgccacgc gatgggtaaa ttccggacta tcgatgatca ccaggacatc
     1321 cggctttgcc gcgatgacgg catcggcggc ttcccggata cgccgcagga ttttcgaaag
     1381 cctgccggga atcgagccaa atccgacgat cgcaagatct ccaagcggaa aaaggctggg
     1441 gacaccttcg gccgccatgt gcgcgccgcc gacacaggag aaatgcgcat ttccctgtgt
     1501 gcgccctctg attgccgaaa ccagcgccgc ccccagccgg tcaccggact cttcgccggc
     1561 gaccagaaaa atatgcgggc tcatgttttc gatccggacg ggacgcccac aacaaagatt
     1621 ccggcacgat ccgcttcacg tacgacgctt tctgcatcgg caattatggt cttggcggag
     1681 acgaccgcaa tccctgccag gcccgcgccg acaacgccgg tcaccgtccg tgggccgatt
     1741 gatggcaagt cgaaccgttg gtcttgatcg ggctttggcg ccttcaccag cacgccggtt
     1801 cccgacgcat cgccgatgcg tccgctggca cgcagttccg cgacccgatc cagcatctga
     1861 tcggtgcctt cggccgcctc aacggccagt atgcgctttc ccgccacaac aacggcctgc
     1921 ccgacatcaa atgcgccgct cgcctgtaaa tagtcaaagc cgaacgcgat atccgcccgc
     1981 tcctgttctg acacatcgat acgacccagc gctccttccg gcaccagaat ttccggcgcc
     2041 acctcgtgcg caccgacaag gcgaaacccc tgctcctcaa ataatttgct gacgccggtg
     2101 agcaggtgat tgtcaccgcc ccggtaagct tgaatgatct tcggcagtga ctttaacgcc
     2161 aaaagatcgg gacgcagcga ccacaaagat ggcctcacca acgcgccgat aaacacaacg
     2221 tcgcggcagt cggccgccct ggcaagccgc gtaaacgccc ccaattgccc aattcgaagc
     2281 caatgatgcg gataacgcgt agtcagcgcg gcgtcggcgt gtccgcgaag cggaaacaaa
     2341 acaacattgc gcccccgctt ggcagccgaa tccgccaccg ccaaaggcaa cgtgcctcca
     2401 ccgcagatca tcgcgagtgg gccgtcctga ccgacggcca cggcgttgct catgactcct
     2461 cttccgtctc gttgcgcttg acggccatgg tcaacggccg tttcgccgtg cgaataaatt
     2521 cgatgatctt ccctataaga
//
LOCUS       DASSRL010000426         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1253706, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000426 DASSRL010000000
VERSION     DASSRL010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253706"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFL49_08630"
     CDS             complement(<1..269)
                     /locus_tag="VFL49_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET7912083.1"
                     /translation="MMILLTLIVGGATRLTESGLSIVEWKPVTGVLPPFSQREWQAEF
                     EKYQAIPQYRELNRGMSLDAFKTIYWWEWSHRLLARLTGAVFLLPL"
     gene            416..631
                     /locus_tag="VFL49_08635"
     CDS             416..631
                     /locus_tag="VFL49_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HET7912084.1"
                     /translation="MSIRTRKLIGAVALLALVIVWGLLAMALAQSVLTNINGWVATIY
                     YIVAGLGWVLPAMPLISWMARPDARPR"
     gene            complement(618..1670)
                     /locus_tag="VFL49_08640"
     CDS             complement(618..1670)
                     /locus_tag="VFL49_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7912085.1"
                     /translation="MKKTIIRTGLETMYFSGMHHWLRPLVGGVGAILTLHHVRPARRE
                     AFQPNRLLEVSPEFLDRLLRRLARSRIDVVSLDEMHRRYIEGDFARRFVCLTFDDGYK
                     DFLRWAYPLLRKYKLPFAMFIATSFPDKLGELWWIALEAVIAQNSRIGMVINGADQYF
                     ECRTARQKRELYGALYGYLRSMKTEEEVRRTVRDLCACYSVDVVSFCRDLCMGWEDIV
                     ELAADPLCTIGAHTVNHMMLRKVPSDATVRAEMDMSRAVLEAALGKRPEHLAFPVGDV
                     TSAGPREFRIAAELGFKTALTTRPGVLFRAHRDYLTALPRISVNGEFQRQRYLKVLMS
                     GTGTAIWNRFRRVSAA"
     gene            1775..2983
                     /locus_tag="VFL49_08645"
     CDS             1775..2983
                     /locus_tag="VFL49_08645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7912086.1"
                     /translation="MNVVAIRPQPVVARNMRASDRRIARVEIFVDLTAAEPHWRALEL
                     GDSLATPYQRYDFLKHWQRHVGSNAGITPFIVVAFNPDGEPLFLLPLGSRPVGGLLGL
                     EFLGGKHANFNMALWRRDVAATIGAAGLRGALAALSGHADVLRLINQPLTWGGATNPF
                     ALLPHQRAANYGFSGALVPDFDALLRARTNSDTRKKMRKKERALASFGELRFERATEP
                     DDVRRVLDAFFKQKSARTRALGMSDAFSLPGVRRFIEAAATEQLNDAKPLIELYALSV
                     DDIIVATMGGIVGGGRFCAMFNSIIQGRFAIESPGEQLILRLVRSCCERELLTFDLGI
                     GEARYKNLLCGDAEPLFDSYIPLSPAGRLPALAFAVSAAAKRAIKNRPALWTLVGSMR
                     RLRARVSSAP"
     gene            complement(2988..>3840)
                     /locus_tag="VFL49_08650"
     CDS             complement(2988..>3840)
                     /locus_tag="VFL49_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET7912087.1"
                     /translation="AAPPEARIISRATPAIKPSYPKKLAIVLIAAFAAFTLSAGFVVT
                     GALLAAPAPSYGYAYSPAVDAPRMKPSPQVVSPPLAPVGPQQMAAPGYGARTIDHVVR
                     DMHNASDSGRVAVIGIVRNVGTTYAAITLARALAQNASVVLVDLAFSAPNLSVMSTDP
                     TAPGVAELTRGRASFGDIITRDQYSRVHLVAAGDVGSDAAALSSSPMLATVIEALGRS
                     YEYVVIDAGSIGDVAPDRIASLATRGMLVAADPSSAVTRSVREQLLRSGFRDVAVVAG
                     GAEAVAA"
BASE COUNT          700 a         1276 c         1189 g          675 t
ORIGIN      
        1 agcggcagca ggaacacggc cccggtcagt ctcgccagca ggcgatgcga ccattcccac
       61 cagtagatcg tcttgaaagc atccaggctc atgccgcggt tgagttcgcg gtattgcggg
      121 atcgcctgat acttctcgaa ctccgcctgc cactcgcgtt gcgagaacgg cggcaatacg
      181 ccggtgaccg gcttccattc gacgattgaa agcccggatt cggtcagccg ggttgcgccg
      241 ccgacgatca acgtcagcag tatcatcatg gccgacaaga gcagccacag ccgaaccggc
      301 cgcaaacgga cactggacgc tttgatcggt tcgtgcatcg tcttgcttgc aggagatggc
      361 gcggaccata tagtccacgg cgccccgccg caattgagag gccggactat cagccatgtc
      421 gatccgaacc cgaaaactga tcggggccgt cgccctcctc gccctggtca tcgtttgggg
      481 cctgctggcg atggcgctcg cgcagtcggt gctcaccaac atcaacggct gggtggcgac
      541 gatctactac attgtcgccg gcctcggctg ggtgctgccc gcgatgccgc tgatcagctg
      601 gatggcaaga ccggacgcta ggccgcgctg acccggcgga accggttcca gatcgccgtg
      661 ccggtgcccg acatcagcac cttcagatag cgctgccgct ggaattcgcc gttgacggaa
      721 atgcgcggca gcgcggtcaa atagtcgcgg tgcgccctga acagcacgcc cgggcgcgtt
      781 gtcagcgccg tcttgaaccc gagctcggcg gcaatgcgga attcgcgcgg acccgctgac
      841 gtcacgtcgc cgacggggaa cgcgaggtgt tcgggccgct ttccgagcgc cgcctcgagc
      901 acggcccggc tcatgtccat ctcggcccgc accgttgcat cgctcgggac tttcctgagc
      961 atcatgtgat tgaccgtatg tgcgccaatc gtgcagagcg gatcggctgc aagctcgaca
     1021 atatcctccc agcccatgca caggtcgcgg cagaatgaaa caacatcgac gctgtagcac
     1081 gcgcacaggt cgcgaaccgt ccggcgcacc tcctcttccg tcttcatgct gcgcaggtag
     1141 ccatagagcg cgccgtagag ttcgcgcttc tgccttgctg tcctgcattc gaaatactgg
     1201 tcggcgccgt ttatcaccat gccgatccgg ctgttctgcg cgatgaccgc ctcgagcgcg
     1261 atccaccaca actcgccgag cttgtcggga aagctggtcg caatgaacat cgcgaacggc
     1321 agcttgtatt ttcgaagcag gggataggcc cagcgcagga agtccttgta gccgtcgtcg
     1381 aacgtcagac agacgaaacg ccgggcgaaa tcaccctcaa tatatcgccg gtgcatttcg
     1441 tcgagagaga ccacatcgat gcgtgagcgc gcgagccgac gcaacaggcg atcgagaaat
     1501 tccgggctga cctccaaaag gcggttcggc tgaaatgcct cgcgtcgcgc cgggcgcaca
     1561 tgatgcagcg tcaggatcgc accgacgccc ccgacgagcg gacgcagcca atggtgcatc
     1621 ccgctgaagt acatcgtctc caggccggtg cggatgatcg ttttcttcaa tgtcggcggg
     1681 ctcccgggcc cctgcggcaa ttcaatttac tatcccaact gtttattgag aaatctttgc
     1741 gaagctcggg cactgttaac cagccctggc agccatgaac gtcgtcgcca tccgccctca
     1801 gcccgttgtc gcccgcaaca tgcgcgcgtc ggaccggcgc attgcgcgcg ttgagatttt
     1861 cgtcgacctg actgctgcgg aaccgcattg gcgggccctc gaactgggtg acagccttgc
     1921 cacgccgtat cagcgctacg attttctgaa gcactggcag cgccacgtcg gctcgaacgc
     1981 cggcataacg ccgttcattg ttgtcgcttt caatccggac ggcgagccgc tcttcctctt
     2041 gcccctcggc tcgcggcccg taggcggcct gctgggactc gagttcctcg gcggcaagca
     2101 tgccaatttc aacatggcac tgtggcgccg cgatgtcgcc gccacaatcg gggccgccgg
     2161 gctgcgcggc gcgctcgccg cgctttcagg acacgcggat gttctcaggc tgatcaatca
     2221 gcccctgacc tggggcgggg ccaccaatcc cttcgcattg ctgccgcacc aacgcgcggc
     2281 aaactacggc ttcagcggcg cgctcgtgcc ggatttcgat gcgctgctgc gggcacgaac
     2341 caattccgac acgcgcaaga aaatgcgcaa gaaggaacgc gcgctggcct ccttcggcga
     2401 gttgcgcttc gagcgggcga ccgagccgga cgacgtgcgg cgcgtgctcg acgctttctt
     2461 caaacagaaa agtgcgcgca cgcgcgcgct cggcatgtcg gatgcatttt cgctgccggg
     2521 cgttcgccgc ttcatagaag cggctgcgac cgaacagctg aatgacgcca agccgctgat
     2581 cgagctctac gcgctgtcgg ttgacgacat catcgttgcg accatgggcg gaattgtcgg
     2641 aggtggccgc ttttgcgcca tgttcaactc aatcatccag ggccggttcg ccatcgaaag
     2701 ccccggcgag caattgatcc tgaggctggt gcggagctgc tgcgaacgcg aacttttgac
     2761 gttcgacctc ggcatcggcg aggcgcgcta caagaacctg ctctgcggcg acgccgagcc
     2821 tctgttcgac agttacatcc ccttgagccc ggccggccgt ctgcctgcgc tcgcatttgc
     2881 ggtcagtgcc gcggccaagc gggcgatcaa aaaccggccc gcactctgga cgctggtggg
     2941 ctcgatgcgg cgtctgcgcg cgcgagtatc gtcagcgccc tgatcgctca ggcggcgacg
     3001 gcctcggcgc cgcccgcaac caccgcgacg tcacggaagc cggatcgcaa cagctgctcg
     3061 cgcacggacc tggtcacggc gctcgaagga tccgcggcga cgagcatccc ccgcgtcgcg
     3121 agcgacgcta tccgatccgg cgcaacatcg ccgatggaac cggcgtcgat caccacatat
     3181 tcgtagctgc ggccgagcgc ttcaataacg gtcgccagca tcggcgacga cgacagcgcc
     3241 gccgcatcgc tcccgacatc cccggccgcg accaggtgca cgcgcgaata ctgatcgcgc
     3301 gtgatgatgt cgccgaacga tgcccttccg cgcgtaagct cggcaacccc cggcgctgtc
     3361 gggtcggtcg acattaccga caggtttggc gcgctgaacg cgagatcgac cagcaccacg
     3421 ctggcattct gagcgagagc ccgcgccaac gtaatcgcgg cataggttgt gccaacgttg
     3481 cgcacgattc cgatcaccgc aacgcgcccg gagtcgcttg cgttgtgcat gtcgcggaca
     3541 acgtggtcga tggtccgcgc gccatatccc ggtgccgcca tttgttgcgg accaaccggc
     3601 gccaatggcg gcgacacgac ctgcggcgac ggcttcatcc gcggggcatc cacggccgga
     3661 ctataggcat aaccatagga cggcgccggc gcggcgagca gagcgccggt aaccacaaaa
     3721 ccggccgaaa gcgtgaaggc ggcgaaagcg gcgatcagca cgatcgcgag cttcttcggg
     3781 tagcttggtt tgattgcggg tgtcgcccgc gaaataatgc gcgcctcggg cggcgcagcg
//
LOCUS       DASSRL010000427         5060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1148433, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000427 DASSRL010000000
VERSION     DASSRL010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148433"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VFL49_08655"
     CDS             <1..1228
                     /locus_tag="VFL49_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HET7912088.1"
                     /translation="AEPDFISVVLKKLRAAETIASNGASLEFRPTSLFAASPPPEIKA
                     IVPIDREQSNSSVIVDNKYVVKVLRRITAGAHPEFEMGRYLADVAHFQNSPALLGTVE
                     LTEGESRTALCAVHVFVQNQGDAWGITGASLDRLIDEQRLVPDEAATETSEMTSMLQR
                     MRQIGRRTAELHRALASYPEADGFTPEPISQEDSARWSDSIMSRAIRVFEMLQENADR
                     LAESAARLAQNLRDHREVICAHIEGLKGSVFNGSKIRVHGDFHLGQVLIAKDDAYILD
                     FEGEPRQSLEQRRRKAPPARDVAGFLRSIDYATSAAIDRAPNITTDERTVLGPRIRVW
                     GARLSEAFLDSYRETLAGMDIWPADEVQAQQLLNLFQLEKAFYEIEYEITNRPTWAHI
                     PLEGTLRILAERGVLA"
     gene            1225..3375
                     /gene="glgB"
                     /locus_tag="VFL49_08660"
     CDS             1225..3375
                     /gene="glgB"
                     /locus_tag="VFL49_08660"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET7912089.1"
                     /translation="MSELSAGAREIIAGRNADPFHYLGPHTEDNRTVVRVFLPDANRV
                     VAVTNGTERELRCIDPAGLFAGPVDDPSHYHLRAHFGDGEVELEDPYRFPPVLSDFDL
                     HLLGEGNHLTLYDKLGAHPMEMEGIAGVAFVVWAPNAQRVSVVGDFNLWDGRRHAMRV
                     RGNGYWEIFVPGARPGEKYKYEISTRSGGLLLKADPVGFAGEMRPLTASVVVDSAKLP
                     RPPRLPAGVNSISAPVSIYEVHLGSWRRKGEHGQYWLDYRELAEQLPAYALDMGFTHV
                     ELLPVMEHPFDGSWGYQPTGLFAPTSRFGTPAEFANLVAALHNAGIGVLLDWVPGHFP
                     DDPHGLANFDGTAIYEHANPMQGRHLDWGTLIYNYGRTEVANFLLANALFWLDRYKVD
                     GLRVDAVASMLYLDYSRPEGGWIPNRFGGRENIDAISFLRRTNTEVFRQFPAATTAAE
                     ESTAWPMVSRPVDWGGLGFGYKWNMGWMHDTLEYISKDPVYRRHHHGNILFGLHYAFS
                     ENFILPLSHDEVVHGKRSILGRMPGDTWQRFANLRAYYSFMFGHPGKKLMFMGSEFAQ
                     DSEWNHDVSLPWHLLQHETHAGIQRLVRDLNRLYRELPALHALDCDTAGFEWLVADDS
                     GNSTFAWLRKGPGALERCVIVVNFTPQILHDYRVRVPFAGRWREALNSDAAIYGGSNA
                     GNAGSVSTVVTRDTPELRIVIPPLAAVFFVPENA"
     gene            3326..>5060
                     /gene="glgX"
                     /locus_tag="VFL49_08665"
     CDS             3326..>5060
                     /gene="glgX"
                     /locus_tag="VFL49_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET7912090.1"
                     /translation="MSSHPLLPSFSFLKTRNGNISAGSPHPLGATWDGRGTNFALFSA
                     NATKVELCLFDREGRRETERIVLPERTDDVWHIYLSHVAPGQLYGYRVHGPYAPEAGH
                     RFNAHKLLIDPYSKRLGGHFVWNDAHFGYRTGHKQADLSFDRRDNSRFTYKSVVVDVA
                     HTWSRERPPATPWEDTIIYEAHVRGLTQRREDVQPYSRGTFRGLSEPGIIAHLRKLGV
                     SAIELLPIHAFLNDRYLLDRGLKNYWGYNTLSFFAPETRYAAGNAITEFRSTVAALHD
                     AGIEVILDVVYNHTCEGNQLGPTLCYRGVDNASYYWLLPDNPRYYDDFTGTGNSLKLA
                     HPRVLQMVMDSLRLWVEVFHVDGFRFDLATTLARRPAFDSSAPFFAAISQDPLLCNVK
                     LIAEPWDIGTGGYQVGGFPTGWSEWNDFYRKTVRRFWRGEGNLIGDLAHCMTASAAQF
                     QHNGRGPRSSINHVTVHDGFTLADLVSYEHKHNEANGEENRDGSDGNLSTNCGVEGAT
                     DDPDILVMRRKLRRNQLACLFLAQGVPLLLAGDEVANSQNGNNNAYCQDNETGWVDWS
                     RLGSDEDLTDYV"
BASE COUNT          988 a         1584 c         1500 g          988 t
ORIGIN      
        1 ggcggaaccc gatttcatct ccgtagtgct gaaaaaactt cgtgccgcgg aaaccatcgc
       61 gagcaatggc gcatcgctgg aatttcggcc aacgtcgctc ttcgccgcgt ccccgccccc
      121 cgagatcaag gcgatcgtcc cgatcgaccg agaacaatca aacagcagcg tgattgtcga
      181 caacaaatac gtcgtcaaag tcctgcgtcg catcacggcg ggcgcccatc ccgagttcga
      241 aatgggccgc tatcttgccg atgttgcgca ctttcagaat tcacctgcct tgttggggac
      301 cgtcgaattg accgagggcg agagtcggac cgcactctgc gcggtgcatg tattcgtcca
      361 gaatcagggc gacgcctggg gtatcacagg cgcctcattg gaccggctca tcgacgagca
      421 acgcctggtt ccggacgaag ctgcaaccga aacgtcggaa atgacgtcga tgctgcagcg
      481 catgcgtcag atcggtcggc gcaccgcaga actgcatcgc gctctcgcaa gttatcccga
      541 ggccgacggc ttcacgccgg aaccgatctc tcaggaagac agcgcccggt ggagtgattc
      601 catcatgtcc cgcgcgatcc gtgtttttga aatgctgcag gaaaacgcag accggctcgc
      661 ggagtcggcc gcccggctcg cgcaaaacct gcgggatcat cgcgaagtga tctgtgcgca
      721 catcgaaggg ctcaaaggca gcgttttcaa tggaagcaag atccgtgtgc acggcgattt
      781 ccacctcggc caggtcctta tcgccaaaga tgacgcctat attctcgact tcgagggcga
      841 accgcgtcag agcctcgaac agcgtcggcg caaggcaccg ccggcgcgtg acgtcgcggg
      901 attcctccgc tcgatcgact atgcgacaag cgccgcaatc gatcgagcgc ccaacataac
      961 gaccgacgag cgtacagtcc tcggaccacg gattcgcgtt tggggcgctc gcctgtcgga
     1021 agcttttttg gattcgtacc gcgagacgct tgccggaatg gatatttggc ctgccgatga
     1081 ggtccaggcg cagcaactat tgaatctttt ccagctcgaa aaggcatttt acgaaatcga
     1141 atatgagatc accaaccggc cgacctgggc tcacatcccc ctggaaggca cgctgaggat
     1201 tctggcagag cgcggagtcc tggcatgagc gagctttccg ccggcgcgcg cgagatcatt
     1261 gcaggccgca atgccgaccc gttccactac ctcgggccgc acaccgagga taaccgtacc
     1321 gtggtgcgcg tcttcctgcc ggacgccaac cgggtcgtcg ccgtgacaaa tggcacggaa
     1381 cgggagttga ggtgcatcga tccggccggc cttttcgcag gccctgttga cgacccctct
     1441 cattaccatt tgcgcgcaca cttcggcgac ggtgaggtcg aactggagga cccctaccgt
     1501 ttcccgccgg ttctttccga ctttgatctc catttgctcg gggaaggcaa tcatctgacg
     1561 ctgtatgaca agctcggcgc acacccgatg gagatggaag gtatcgcggg cgttgccttc
     1621 gttgtgtggg cgcctaacgc ccagcgcgtc agcgtcgtcg gcgatttcaa tctatgggat
     1681 ggacggcgcc atgcgatgcg cgtgcgcggg aacggctact gggagatttt cgtgccaggc
     1741 gcgcgacctg gcgaaaaata caaatacgaa atcagcacgc gatcaggcgg tctgctgctg
     1801 aaggccgacc cggtcggctt tgccggcgaa atgcgtcccc tgaccgcttc ggtcgtcgtc
     1861 gatagtgcga agctcccgcg tccaccgagg ttgccggcgg gcgtcaactc catcagcgcg
     1921 cccgtgtcga tctacgaggt gcatctgggc tcctggcggc gcaaaggcga gcacggccag
     1981 tactggctcg attatcgcga actcgccgag caattgcccg cctatgcgct cgacatgggg
     2041 tttacgcatg tcgaattgct tccggtgatg gagcaccctt tcgacggttc gtggggatat
     2101 caacccaccg gcctatttgc gccgacaagc cgcttcggca ctcccgcgga attcgcgaat
     2161 cttgtcgcgg ccctgcataa cgccggcatc ggtgtcctgc ttgattgggt gccggggcat
     2221 tttccggacg atccccacgg gctggcaaac ttcgacggca ccgcgatcta tgagcatgcc
     2281 aacccgatgc aagggcggca cctcgactgg ggcacgctca tctacaacta tggacggacc
     2341 gaggtcgcca acttccttct cgcgaacgcg ctcttctggc tcgatcgcta taaggtcgac
     2401 ggactgcgcg tcgatgccgt cgcctcaatg ctctatctcg actacagccg cccggaaggc
     2461 ggctggatcc cgaaccggtt tggcggccgg gaaaacatcg acgccatctc tttcctccgg
     2521 cgcaccaata ccgaggtgtt caggcaattt cccgctgcga ccacggccgc cgaggaatcc
     2581 acggcctggc cgatggtgtc ccggccggtc gactggggcg gccttggctt cggctacaaa
     2641 tggaacatgg gatggatgca cgacacgctg gagtacatct ccaaggatcc ggtctaccgg
     2701 cgccatcacc acggtaatat cctgttcggc ctgcactacg cgttttcgga aaacttcatt
     2761 ctgccgctgt cgcacgatga agtcgtgcac ggaaaaaggt cgatcctcgg ccgcatgccg
     2821 ggcgatacct ggcagcgctt cgcgaatttg cgcgcgtact attccttcat gttcggccat
     2881 cccggcaaaa aactgatgtt catgggctcc gaattcgccc aggacagcga atggaatcat
     2941 gatgtcagcc tgccctggca cctgctgcag catgagactc acgcaggtat ccagcggctg
     3001 gtgcgcgatc tcaaccggct ctatcgcgag ttgccggcgc tgcatgcgct cgactgcgac
     3061 actgccggct tcgaatggct ggtcgccgac gactccggca acagtacctt tgcctggctt
     3121 cgaaaggggc cgggcgcgct ggagcgttgt gtaatcgttg tcaatttcac cccgcagatt
     3181 ctgcacgact accgcgtccg cgtgcctttt gccggccgct ggcgagaggc gctgaactct
     3241 gacgctgcaa tctacggcgg aagcaatgcg gggaacgcgg ggtcggtatc taccgttgtc
     3301 acgcgcgaca ctccggagct tcgcattgtc atcccacccc ttgctgccgt ctttttcgtt
     3361 cctgaaaacg cgtaacggca acatctcggc agggtctccg cacccgcttg gcgcgacctg
     3421 ggatggccgc gggacgaact ttgcgctgtt ttccgccaat gccacgaagg tcgagctatg
     3481 tctgttcgat cgcgagggcc gtcgcgagac cgaacgcatc gtgctgcctg aaagaaccga
     3541 cgacgtgtgg cacatttacc tttcacacgt cgcccctgga caattgtacg ggtatcgggt
     3601 gcatggccct tacgcaccgg aggcgggtca tcggttcaac gcgcacaaac tgctcatcga
     3661 tccctacagc aagcgtctgg gcggccattt tgtctggaac gacgcgcatt tcggctaccg
     3721 gacgggacat aagcaggccg acctctcgtt cgaccggcgc gacaattccc gcttcacata
     3781 caaatcggtc gtcgttgacg tggcgcacac gtggagccgc gaacggccgc ccgcaacgcc
     3841 atgggaagac acaatcatct atgaagcgca tgtccgcggt ctcacgcagc ggcgcgagga
     3901 tgtgcagcct tactcacgcg gaacgtttcg cggcctgtcc gagccgggga tcatcgcgca
     3961 cttgcgcaag ctcggcgtca gcgcaatcga actgctgccc atccatgcct ttcttaacga
     4021 ccgttatttg ctggaccggg ggctaaagaa ctactggggc tacaacacgc tgagcttctt
     4081 tgcgcccgag acgcgttacg ccgccggcaa tgcaattacc gaattccggt cgacggtggc
     4141 ggcactgcac gatgccggca tcgaggtgat cctggacgtc gtctacaatc acacttgcga
     4201 aggcaaccag ttgggaccaa cgctttgcta ccgcggggta gataacgcgt cgtattactg
     4261 gctgctgccc gacaatcccc gctattacga cgatttcacc ggcaccggta attcgctcaa
     4321 gctcgcgcac ccccgcgtgt tgcagatggt gatggattcg ctgcgcctgt gggtggaggt
     4381 tttccatgtc gacgggtttc gtttcgatct tgccaccaca ttggcccgtc gcccggcctt
     4441 cgacagcagc gcgccattct ttgcggcaat cagccaggat ccgctcctgt gcaacgtcaa
     4501 actgattgcc gagccctggg acatcggcac aggcgggtac caggtcggcg gctttccgac
     4561 cggctggtcg gaatggaatg atttctatcg caagacggtg cgccgcttct ggcgcggcga
     4621 aggcaacctg atcggcgacc ttgcgcattg catgaccgct tcagcggcgc aattccagca
     4681 caacgggcgc ggcccgcgca gcagcatcaa tcacgtcacc gtgcatgacg gcttcaccct
     4741 cgccgacctc gtcagctacg agcacaagca caacgaagcg aacggcgagg agaaccgcga
     4801 cgggtccgac ggtaatctga gcacaaattg cggcgtggaa ggcgcaaccg atgatccgga
     4861 tatcctggtc atgcggcgaa agctgcggcg caatcagctc gcctgccttt ttctcgcgca
     4921 aggagtgccg ttgctgctgg ccggcgatga agtcgcgaac agccagaacg gaaataacaa
     4981 tgcgtattgc caggacaacg agacaggctg ggtcgactgg tcacgcctcg gcagcgacga
     5041 ggacctgacc gactatgtcg
//
LOCUS       DASSRL010000428         5091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_186291, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000428 DASSRL010000000
VERSION     DASSRL010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186291"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1090
                     /locus_tag="VFL49_08670"
     CDS             209..1090
                     /locus_tag="VFL49_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET7912091.1"
                     /translation="MSSLSGKDGPRIRNTAGASQPYETIGVDRLTPIIGAEIDGVDLA
                     KPLSNRTVDEIHRALAENIVIFFRKQHITPQQHLAFGRLFGELHVHPAAPHEPGEPAL
                     MKIYADKNSPRANGEGWHSDVSCDLEPPMGSILYIRQCPPHGGDTLFASMYAAYDALS
                     DRMKTYLEGLTAVHDGEMVYRGLYANTGVADKPTYPRAEHPVVRTHPVTNKKALYVNR
                     GFTSHINGIPRDESDAILSYLYQHAENPLFQCRFRWTENAIAFWDNRCSQHRAMWDYW
                     PHTRAGTRVTVKGERPV"
     gene            1126..1329
                     /locus_tag="VFL49_08675"
     CDS             1126..1329
                     /locus_tag="VFL49_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912092.1"
                     /translation="MPAHETEDRLRAERLFNVREKQKADAPQATAEYYAAEQKLRDRT
                     RELRQLRLTREAQTKRQTKRDAT"
     gene            complement(1341..2294)
                     /locus_tag="VFL49_08680"
     CDS             complement(1341..2294)
                     /locus_tag="VFL49_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7912093.1"
                     /translation="MLRIFTILLFCILILPAAQSHAQSSFKVTLLGTASPSPRPTRMG
                     PSTLVEAGTQRLLFDAGRGVPVRLWQIKVPMGTIDALFITHYHSDHVSGIPDLWLTGW
                     LSPPFGRRKTPFHVIGPTGAKSLMENLDKAYALDKSIRIADEKLPPEGVAIKVDEFDK
                     DGVVYEKDGVKVIAFKVDHGAAIKPAYGYRIEYNGHAVTISGDTRYNQNVIKYGTGAD
                     VLIHEVGMARPELMSNVFVQRIIDHHTTPKDVGRVFTQAKPKLGVYTHIVLLSSEKIP
                     EPTLDDLVAETRQTYSGPLEVGEDLTTFEIGDTVTVKRFKQ"
     gene            complement(2374..3537)
                     /locus_tag="VFL49_08685"
     CDS             complement(2374..3537)
                     /locus_tag="VFL49_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HET7912094.1"
                     /translation="MLLGVALFHRHTLPIALAGLGVITAYKLIFTGFREGAGPAGLGA
                     HLGHEFSLLSNLFLLLMGFALLSRHFEESAVPDRVPGLLPHNWTGGLLLLGIIFVLSA
                     FLDNIAAAIIGGTIARHVFGGKVHVGYLAAIVAASNAGGAGSVVGDTTTTMMWVAGIS
                     PLAVVEAYVAAVAALVIFAVPASLQQQRHSPIVKRTQHDVAIDWTRVLIVLAILFAVV
                     ATNVLANMRFPAVLGRVPVLGLSLWAVVIATAPLRRPDWSVLPETLKGTVFLIALVTA
                     ASMMPVETLPGASWQTALGLGFVSAVFDNIPLTALALKQGGYDWGYLAYAVGFGGSMI
                     WFGSSAGVALSNQFAEAKSVGRWLRHGWHVALAYVVGFFLMLALIGWHPNPGP"
     gene            3743..4738
                     /locus_tag="VFL49_08690"
     CDS             3743..4738
                     /locus_tag="VFL49_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7912095.1"
                     /translation="MPVYSQTFSKTISRRGVLGTAAVLAETPAFAVCAIGPPKHDKGP
                     HVWMDLDQVELDAAYDQSVYAPLLRQILKRYASSSDEARGRLGTPKRLAYGPTAVEAL
                     DLYPARTANAPVFVFIHGGAWRSGAAKDYGFPAELFVNAGVNYIALDFIAVGAANGDI
                     GAMADQVRRGVAWAYKNTASYGGDAGRFYIGGHSSGGHLCGIALTTDWQKDFGLPADM
                     VKGGLCMSGIYDMKPVRLSKRSSYVKFTDAMEEAMSSQRQIEKLRAPIVVTYGSHETP
                     EFQRQSRDFAAAVKTAGKPVELVEAANYDHFEMCESLGNPYGPNGRAALRLMKLA"
     gene            4853..>5091
                     /locus_tag="VFL49_08695"
     CDS             4853..>5091
                     /locus_tag="VFL49_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912096.1"
                     /translation="MTKPFLLLAGIAAGFALAGAGAMNATDAWAQKPLAKPVQSQPQQ
                     ADKDSQSFIKKAIEGDIAEVDVGKLAEEKSKSEAV"
BASE COUNT         1045 a         1591 c         1607 g          848 t
ORIGIN      
        1 gtcttcggag agaaaaggcg gcataaccag caagaatcgc gcgtgcgcaa tgtgagcagg
       61 ccagctggag gcgactgcag ccgatcgcct gcttagccgc acgcgggatg ggccggaatc
      121 ggccccctcc accggctcat attgttaagt tgcgcgcgcc cgcgaccgcg ctaccatccc
      181 ggcaaaggac ttcgccggga ggttccacat gagctcgctg agcggcaagg acggcccgcg
      241 catccgcaac acggccggcg catcgcagcc ttatgagacg atcggagtcg acaggctcac
      301 gccaatcatc ggcgcggaga tcgatggcgt cgatctcgcc aagccgctgt cgaaccggac
      361 ggtagacgag atccaccgtg cgctggccga gaacatcgtc attttcttcc gcaagcagca
      421 catcacgccg cagcagcatc tcgccttcgg ccggctattc ggcgagctgc atgtgcatcc
      481 ggccgcgccg cacgagcccg gcgagccggc gctgatgaaa atctacgccg acaaaaacag
      541 cccgcgcgcg aacggggagg gctggcacag cgatgtgtcc tgcgatctcg agccgccgat
      601 ggggagcatc ctctatatca ggcaatgccc gccgcatggc ggcgacacgt tgtttgcgag
      661 catgtatgcc gcctacgacg cgctgtcgga ccggatgaaa acttacctcg aggggctgac
      721 cgcggttcac gacggcgaga tggtctatcg cgggctttat gcgaacacgg gggtggccga
      781 caagccgacc tatccgcgcg ccgagcatcc ggtcgtgcgc acgcacccgg tgacgaacaa
      841 gaaggcgctc tatgtcaacc gcggtttcac ctcgcacatc aacggcattc cacgtgatga
      901 gagcgacgcg atcctgtcct atctctatca gcatgcggag aatccgctgt tccagtgtcg
      961 cttccgctgg acggagaatg cgatcgcctt ctgggacaac cgctgttcgc agcaccgcgc
     1021 catgtgggac tactggccgc acacgcgcgc gggcacgcgc gttacggtca agggcgagcg
     1081 tccggtctga cgggaactgt tgccggcctt tcgcgttaac gttgcatgcc cgcacatgaa
     1141 accgaagacc ggctccgcgc cgagcgtctg ttcaacgtac gcgaaaagca aaaagccgac
     1201 gcgccgcagg cgaccgcaga atactacgcg gccgagcaga agcttcgcga ccggacgcgg
     1261 gagctgaggc agttgcggct cacaagggag gcgcaaacga aacgccagac aaagcgtgac
     1321 gccacctagc gaactgcggc ctattgcttg aaccgcttca ccgtcaccgt gtcgccgatc
     1381 tcgaatgtgg tcagatcctc gcccacctca agcggcccgg aataggtctg cctcgtctcg
     1441 gccacgagat catcgagcgt cggctccgga atcttttcac tgctgagcag gacgatatgc
     1501 gtgtagacgc cgagcttcgg ctttgcctgg gtgaaaaccc ggccgacatc ctttggcgtg
     1561 gtgtgatggt cgatgatgcg ctgcacgaag acgttcgaca tcagctccgg ccgtgccatc
     1621 ccgacctcgt ggatcaggac gtccgcgccg gtgccgtact tgatgacgtt ctgattgtag
     1681 cgcgtgtctc cggaaatggt gacggcatgg ccattgtatt cgatccggta gccataggcc
     1741 ggcttgatgg ccgcgccatg atcgaccttg aaggcgatga ccttgacgcc atccttttca
     1801 tagacgaccc cgtccttgtc gaactcgtcg accttgatcg cgacgccttc cggcggaagc
     1861 ttctcgtcgg cgatgcggat cgatttgtcg agcgcgtagg ctttgtccag attctccatc
     1921 agcgattttg cgccggtcgg cccgatcaca tgaaacggtg tcttgcgccg gccgaacggc
     1981 ggcgacagcc agccggtcag ccagaggtcc gggatgccgg agacgtggtc cgagtgataa
     2041 tgggtgatga agagcgcgtc gatcgttccc atcggcacct tgatctgcca cagccggaca
     2101 gggacgccgc ggccggcatc gaatagaagg cgctgcgtgc ccgcctcgac cagcgtgctc
     2161 ggccccatcc gcgtcgggcg cggcgacggc gacgcggtgc ccagcagcgt caccttgaaa
     2221 gagctctggg catggctttg ggccgccggc aggatcaaga tgcaaaaaag aaggatcgta
     2281 aaaatgcgga gcatgaacgc ctcccacaga attctgtgca gcaattgttc aaggttctca
     2341 cacccttcgc aagccgatcc gcatcggaaa atctcaaggt cccggattgg gatgccatcc
     2401 gatcagcgcc agcatgagga agaatccgac cacgtaagcg agcgcaacat gccagccgtg
     2461 gcgcagccac cggccaaccg attttgcctc ggcgaactgg tttgacagtg caaccccggc
     2521 cgaggagccg aaccagatca tcgagccgcc gaacccgacc gcataagcga ggtagcccca
     2581 atcgtagccg ccttgcttaa gcgccagcgc cgtcagcggg atgttgtcga acacggccga
     2641 gacgaagccg aggccgagcg ccgtctgcca tgatgcgccc ggcagcgttt cgaccggcat
     2701 catcgaagct gccgtaacca aggcgatcag gaagacggtg cctttcagcg tctccggcag
     2761 cacgctccag tccggccgcc gcagcggcgc cgtagcaata acgaccgccc acagcgagag
     2821 accgagcacc ggcacccgtc cgagaaccgc ggggaaccgc atgttggcaa ggacattggt
     2881 cgcaacaacg gcgaagagga ttgcgaggac gataaggacc cgtgtccagt caattgccac
     2941 atcgtgctgc gtgcgcttga cgatgggtga atgccgctgc tgctgcaggg aagcggggac
     3001 cgcgaaaatc accagcgccg cgacagcggc gacataggcc tccacgacgg ccagcgggct
     3061 tattcccgca acccacatca ttgtcgttgt cgtatcgccg accacgctgc cggcgccgcc
     3121 ggcgttggag gcggcgacga tggcggcgag gtaaccgaca tggaccttgc cgccgaacac
     3181 gtgccgcgcg atcgtgccgc cgatgatcgc cgcggcgata ttgtccagga aggccgacag
     3241 cacgaagata atgccgagca gcagcaggcc gcccgtccag ttgtgcggca gcaggccggg
     3301 cacccggtcc ggcacggcgc tttcctcgaa atgacgcgac agcagcgcaa atcccatcag
     3361 cagcaagaac aggttcgaaa gcagcgaaaa ttcgtggccg agatgcgccc cgagcccggc
     3421 cggcccggcg ccttccctga agccggtgaa gatcagtttg taggccgtga tgacgccaag
     3481 gccggcaagc gcgatcggca gcgtgtgccg gtgaaacagc gcgacgccaa gcagcatcag
     3541 cgcaaacagc acgaactcga agggaatgcc gaagacggtc aaaacggcct caaccgattt
     3601 ggttgtacgg caggaataca acaggtaggc ggagaatgct tgcggttcgg ggtgatttaa
     3661 tgccgcattc gccccaatgg gagtctggcg atcagcgggc gcggcttgct aacatgccgc
     3721 agatcgcgag gggagaatga caatgccggt ctacagtcaa accttttcga aaacgatatc
     3781 gcgccgcggt gtgctcggca cggccgctgt actggcggaa acgcccgcct ttgccgtttg
     3841 cgcaatcggc ccacccaagc atgacaaggg accacatgtc tggatggacc tcgatcaggt
     3901 cgagctcgac gccgcctacg atcaaagcgt ctatgctccg ttacttcggc agattctcaa
     3961 gcgttatgcc tcgagcagcg acgaggcacg cggccgtctc ggcacgccga agcggctcgc
     4021 ttacggaccg acggcggtcg aggcgctcga tctctatccc gccaggacag ccaatgcgcc
     4081 ggtcttcgtg ttcatccatg gcggcgcctg gcgcagcggc gcggcaaagg actacggctt
     4141 tccggccgag cttttcgtca atgccggcgt gaattacatc gcgcttgatt tcatcgcggt
     4201 cggcgcggcg aatggcgaca tcggcgccat ggccgaccag gtgcggcgcg gcgttgcctg
     4261 ggcctataaa aataccgcga gctacggagg cgacgccggc cgtttttaca tcggtggcca
     4321 ttcctccggc ggccatctct gcggcattgc cctgaccacc gactggcaaa aggatttcgg
     4381 gttgccggct gacatggtga agggcgggct ctgcatgagc ggcatctacg acatgaagcc
     4441 ggtgcgcctc tccaagcgca gctcctacgt caagttcacc gatgcgatgg aagaggcgat
     4501 gagctcgcag cgccagatcg agaagctgcg cgcgccgatc gtcgtgactt acggcagcca
     4561 cgagacgccc gagtttcagc gccagagccg cgattttgcc gctgcggtga agacggccgg
     4621 caagccggtc gagctggtcg aggcggcgaa ttacgatcat ttcgagatgt gcgagtcgct
     4681 tggcaatcct tacggtccga acggccgcgc tgcgctcagg ctcatgaagc tcgcttgacg
     4741 ccacagtttt tgtggcgcgc cacgcctgct ggaaccagga cattcgcctc gcggttgtcg
     4801 tgacggcctt ccggctgacc ggaagccgca atcatgaccc cgaggtgaga caatgacgaa
     4861 accattttta cttttggccg gcatagcggc tggttttgcg ctggccggag ccggcgcaat
     4921 gaatgcaacg gacgcctggg cgcaaaagcc cttggccaag ccggtgcagt cacagccgca
     4981 acaggcggac aaggacagcc agtccttcat caagaaggcg atcgagggcg acatcgccga
     5041 agtggatgtc ggcaagcttg cagaagagaa gagcaagagc gaggccgtga a
//
LOCUS       DASSRL010000429         6393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_81516, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000429 DASSRL010000000
VERSION     DASSRL010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81516"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /gene="asnB"
                     /locus_tag="VFL49_08700"
     CDS             complement(<1..1134)
                     /gene="asnB"
                     /locus_tag="VFL49_08700"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET7912097.1"
                     /translation="MCGIAGLIDTRLTGQPDRLRALGQKMAEALAHRGPDASGVWADA
                     AASLVLSHRRLSIVDLSAAGAQPMTSADERWVISFNGEIYNANRIAAAPELAHCNWRG
                     HSDTEIILESFARRGVDATLADMNGMFAIALWDRRQQTLYLIRDHLGIKPLYVLEDDG
                     RLAFASELKAFAQLDRWSPQIDMASVTSFFRFGCVPAPFSIFKDVRKLMPGEVMTISP
                     GKRPVARRYWSIATVAELGARSTLDLSDAEAVRQFDELLSDAVAQQMMSDVPIGAFLS
                     GGIDSSTVTACMMQAGRGPVRTFSVGSPDLNFDESRQAAVIAKHLGTDHTELTVTARD
                     AMNTIDSLPEMYDEPFADSSQIPTFLISRLTRQYVTVALSGDGG"
     gene            complement(1279..2118)
                     /locus_tag="VFL49_08705"
     CDS             complement(1279..2118)
                     /locus_tag="VFL49_08705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET7912098.1"
                     /translation="MSMRNASATVQDTIRSLQWQTQGDWELVLFDDGSTDDSVGVVQT
                     ISDKRIRLQKDGKRKGLAARLNEAVALARGRFVARIDADDICFPARLDKQVAYLESHP
                     GVDVVASRAAVFSGRDLIGVMPVGSDHWDIVREPFRGFPFPHPTWCGRAEWFRANPYD
                     GSLGKTQDQDLLLRTCARSTFAGMPDILVGYRQPKLEIRKLLRGRFIFARSLWRHGRA
                     LAPLSQIIGGMAMQVVKGTADVVTLGLGLNRWGQLQRLQSVPRSLQDEWPALYERLQS
                     GAA"
     gene            complement(2160..3383)
                     /locus_tag="VFL49_08710"
     CDS             complement(2160..3383)
                     /locus_tag="VFL49_08710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7912099.1"
                     /translation="MKILCVFGRYAYGDPQRGDGYEYVNFLPALAALGHEVELFDSFD
                     RRGYVDFADLNQRLLDHVIAFRPDVVFCVLMNYEVWSETLDIIRAGSPAIVVNWGTDD
                     SWKFHQVSRFLIPHLDFHVTTDKGAAGWAGGAGFANVLRSQWAASPAQLREPLPSSQC
                     TYDVSFIGNLYGDRRAWIEGLRARGVHVTTFGHGSDGGVVASPAIPDIHRKSRISLNF
                     SDSGLQLDGLRLKRSRQIKARVFEVPGAGSVLLTQAADGLSDYFRIGDEIDTFDRPDG
                     LAEKIKFYLANPDVRDRLARAGQQRVVAEHTYDRRFRDLLAYVQTHAVPARARQAWQI
                     DRAEMQRLVDLHRRTAAIKPLRVAVNTMTRLLLGQEKGARGARRIVFEASLRLAGGRT
                     FGAAGLPGRLFFRES"
     gene            complement(3380..4138)
                     /locus_tag="VFL49_08715"
     CDS             complement(3380..4138)
                     /locus_tag="VFL49_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7912100.1"
                     /translation="MSKNRSALSRLGYNDLFAFNLRNRDRWIAAQAAQIPAGARVIDI
                     GAGSAPYRPAFAHCQYKTQDFKSLAGSQLRHGEYASIDYVCDAKAIPVAPGSFDVALC
                     AEVLEHHPEPIAVVQEIARILAPGGRLLLTAPLGSGIHQEPYHFYGGYTPYWYRQFLH
                     AAGFEDIEVVANEGSFRHFAQESIRFCRMSRPFALGMPLLAELAWLPFWCLLAPVLGF
                     AVPLAAKLLDRFDREQRFTIGYHVRATKGSGAAA"
     gene            complement(4135..4878)
                     /locus_tag="VFL49_08720"
     CDS             complement(4135..4878)
                     /locus_tag="VFL49_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF268 domain-containing protein"
                     /protein_id="HET7912101.1"
                     /translation="MLRDFGRSWVLPFVDPRQLASVVRLPGFFADWLRFRGLAGKDTA
                     RFADLRPCLTDRAGHTPFDPHYFYQAAWLARELNGARPAMHVDVGSSVVATGVLSAFV
                     PTLFVDFRPLLARLPNLDSVAADITRLPFADGSVQSLSSLHVIEHIGLGRYGDRIDPD
                     GWRAGLSELARVLAPGGRLYLSTPVGRNRVCFNAHRVFLPAAIVGALPSLKLERFATV
                     DDEGCRKDNAELTEADTMSYGCGMFRFSK"
     gene            complement(4878..5720)
                     /locus_tag="VFL49_08725"
     CDS             complement(4878..5720)
                     /locus_tag="VFL49_08725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7912102.1"
                     /translation="MRISVVSPNYNMAGYLGETISSVRRNLAPGDEYIIVDGGSTDGS
                     VDVIRKHEASITHWISERDEGYADAIGKGFKRASGDVLCWINAGDLLLDGTLAKVREL
                     FAAGDADMIFGDDFYIDEQSRVIRYSRGYVNDLASAMLHGGWTPLQDACFWTRDIYMR
                     AGGIDRKLHLAADYDLFLRIARAGRARHVPFAFSAFRRHAGQKSSDASGYAKERERVR
                     HREMKSAADGAMAAVAKRQFHLNAGRLRAWLAPRIRPRRDLEGHPIRDLRCAAYWPAG
                     NPSN"
     gene            complement(5737..>6393)
                     /locus_tag="VFL49_08730"
     CDS             complement(5737..>6393)
                     /locus_tag="VFL49_08730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7912103.1"
                     /translation="LLPHPTPRFALDAGPGTPAPQDIAHLKIRRPYFLYPAQFWAHKN
                     HVNLVLALAELVSRHGLDVDFAFVGSDKGNRDHVWSVAKEAGVADRLNYLGFVSRDDL
                     VHLYRNAIGLAYVSWCGPENLPPLEAVALGCPVVATRIPGAEEQLGGAALFVDPARPD
                     DIADGLARIHSDAALRAALVSKGLARARRWTSLEYVSGAMDIFTNLEPMLRCWRAGQV
                     "
BASE COUNT         1261 a         2036 c         2016 g         1080 t
ORIGIN      
        1 gccgccgtcg ccggacagcg caaccgtgac gtattggcgg gtgagcctgg aaatcaggaa
       61 cgtcggaatc tgcgaggaat ccgcgaatgg ctcgtcatac atttccggca gcgaatcgat
      121 ggtgttcatc gcgtcccttg ccgtcaccgt gagctcggta tggtcagtcc cgagatgctt
      181 cgcaatgacg gccgcctgcc tggactcgtc gaaattcaga tcgggtgagc cgaccgaaaa
      241 tgtgcggacc ggtccgcggc ccgcctgcat catgcaggcg gtcaccgtcg acgaatcaat
      301 tccgcccgaa agaaatgcgc cgattggaac gtcgctcatc atttgctgcg ccacggcgtc
      361 ggacagaagc tcgtcgaact gccgcacggc ttccgcatct gagaggtcaa gcgtgctgcg
      421 cgcgccgagt tcggcaacgg tcgcaatgga ccaatagcgc cgtgcaaccg gccgcttccc
      481 cggcgatatc gtcatcacct cgcccggcat cagcttgcgc acgtccttga agatgctgaa
      541 cggcgccggc acacagccga accggaaaaa gcttgtcacc gaggccatat cgatctgcgg
      601 cgaccagcgg tcgagctgag cgaaggcttt aagctccgac gcaaaagcca gccgtccgtc
      661 atcctcgagg acgtagagag gcttgatccc gaggtgatcg cggatgaggt aaagcgtttg
      721 ctgccggcgg tcccacaatg cgatggcgaa catgccgttc atatcggcca aagtcgcatc
      781 cacgccacgg cgcgcgaatg attccaggat gatttcggtg tccgaatggc cgcgccagtt
      841 gcaatgcgcg agttccgggg cggcggcgat cctgttcgca ttgtagatct cgccgttgaa
      901 gctgatgacc cagcgttcgt cggcggacgt catcggctgc gcgcccgccg cggagaggtc
      961 gacgatggac aggcgccggt gcgacaacac gaggctggcc gcagcgtccg cccacacgcc
     1021 gctggcgtcg gggccacggt gagcaagcgc ttctgccatc ttttgcccga gtgctctgag
     1081 cctgtcgggt tgtcccgtca ggcgcgtgtc gatcaggccg gcaattccgc acatagcaga
     1141 ttgcaatccg tcgtggctgg aacgatctcg ctaccgctca ttcccgcgaa agcgggaatc
     1201 cagcattttt cgactctggg tccccgcttt cgcggggacg agcggataac aatcgatcca
     1261 gttcgagtgc atcaaattct aggcggcgcc cgactgtaag cgttcgtaga gcgccggcca
     1321 ttcgtcctgc aggctgcgcg ggacggattg cagacgctgg agctggcccc agcggttgag
     1381 gccgagccca agcgtgacaa cgtccgccgt cccttttacg acctgcatcg ccattccgcc
     1441 gatgatctgt gaaagcggcg caagcgcgcg tccatgccgc cacagcgatc gcgcaaagat
     1501 gaaacggccg cgaagaagtt tcctgatttc gagcttgggc tgccggtatc cgaccaaaat
     1561 gtcgggcatg ccggcgaatg tgctgcgggc acaggtgcgc agcagcagat cctggtcctg
     1621 ggtcttcccg agcgatccgt catagggatt tgcacgaaac cattccgcac gcccgcacca
     1681 ggtcggatgc ggaaacggga atccccgaaa cggctcacgg acgatgtccc aatggtccga
     1741 cccgacgggc atcacgccga tcagatcgcg gccggaaaac acggctgcgc gcgatgccac
     1801 gacgtcgacg cccgggtggc tctcaagata ggccacctgt ttatccaacc gtgcgggaaa
     1861 gcagatgtcg tcggcatcga tgcgcgcgac gaaccgtccg cgcgcaagcg ccactgcctc
     1921 gttgagacgt gcggcaagtc ctttacgttt tccgtctttt tgcaaccgaa ttcgcttgtc
     1981 ggaaatcgtt tgcacgacgc caacgctgtc gtcggtcgaa ccgtcatcga acaggacaag
     2041 ctcccagtcg ccctgagtct gccactgcaa cgaacggatt gtgtcctgca ctgtggcgga
     2101 tgcattccgc atcgacatga tgacagagac cagcggcctt tcggtcgtca ctgttgcagt
     2161 cagctttcac gaaaaaacag ccggcccggc agacccgccg cgccgaatgt tcgcccgccg
     2221 gcaagccgca aggacgcctc gaacacgatg cgccgcgcgc cgcgcgcgcc tttttcctgg
     2281 ccaagcaaaa ggcgcgtcat cgtgttgacc gccacgcgaa gcggtttgat ggccgctgtc
     2341 ctgcgatgca ggtccaccag gcgttgcatc tcggcgcgat cgatttgcca ggcttgacgg
     2401 gcgcgcgccg gcacggcgtg cgtctgaaca taggcgagaa ggtcgcgaaa gcgccggtcg
     2461 taggtgtgct cggcaacgac acgctgctgg cctgcccgcg ccagtcggtc gcggacatca
     2521 gggttggcca gatagaattt tattttttcc gcaagaccat ccggccgatc gaaagtatcg
     2581 atctcgtcgc cgatccggaa ataatcggac agtccgtcgg cggcctgggt gagaaggacg
     2641 ctacccgctc cggggacctc gaacacgcgt gccttgatct ggcgggagcg cttgaggcga
     2701 agcccgtcca gctgcaagcc tgaatctgaa aaattcagcg atatccgtga cttgcgatgg
     2761 atgtccggaa tggcggggga agcgacaacg ccgccatcgc ttccgtgtcc gaaggtcgtg
     2821 acgtgcacgc cccgggcacg caggccctcg atccaagcgc ggcggtctcc gtacaggttg
     2881 ccgatgaaac tgacatcgta ggtgcattgg ctgcttggca gcggctcccg cagctgcgcg
     2941 ggcgatgcgg cccactgcga gcggagcacg ttcgcaaagc cggcacctcc ggcccagccc
     3001 gccgcgccct tgtcggtcgt cacgtgaaaa tcgaggtggg ggatcagaaa ccgggatacc
     3061 tgatggaact tccaggagtc gtctgtaccc cagttgacga caatggccgg cgaccctgcg
     3121 cggatgatgt cgagggtctc ggaccagacc tcgtagttca tcagcacgca gaagacgaca
     3181 tccggcctga acgctatgac gtggtcgagc aggcgctggt tgagatcggc aaaatcgaca
     3241 tagccccggc ggtcgaacga atcgaacagt tcgacctcat gaccgagcgc ggcgagcgcg
     3301 ggaagaaaat tgacatattc gtagccgtcg ccccgctgcg gatcgccgta ggcatagcgg
     3361 ccgaagacgc aaaggatctt cacgccgcag ctcccgagcc ttttgtcgcg cgcacgtgat
     3421 acccgatggt gaatctctgc tcgcggtcga agcggtcgag aagctttgcc gcgagcggga
     3481 ccgcgaagcc gagcaccgga gcgagcaggc accagaacgg aagccaggcc aattccgcca
     3541 gaagcggcat gccgagcgcg aacggccgcg acatccggca gaagcggatc gattcctgcg
     3601 cgaagtgccg gaacgacccc tcgttcgcga ccacctcgat gtcttcgaaa ccggcggcgt
     3661 ggagaaattg ccggtaccag tacggcgtgt agccaccgta aaaatgatag ggctcctgat
     3721 gtatccccga gcccagcgga gccgtcagca gcaggcgccc gcctggcgcg aggatgcgcg
     3781 caatctcctg caccacggcg atcggttcgg gatggtgctc cagcacctcg gcacagagcg
     3841 caacgtcaaa gctgccgggc gcaacgggaa tagccttggc atcacagaca tagtcgatgg
     3901 acgcgtattc accgtgccgc aactggctgc ccgccaggct tttgaagtcc tgcgtcttgt
     3961 actggcaatg cgcgaacgcc ggacggtatg gcgcggaacc ggcgccgata tcgatcacgc
     4021 gtgcgccggc ggggatttgc gccgcctgcg ccgcgatcca gcgatcccga ttgcgcagat
     4081 tgaatgcgaa gagatcgttg tacccgagcc ggctgagtgc ggagcggttc ttgctcattt
     4141 tgaaaagcgg aacatgccgc agccatagct catcgtgtca gcttcggtga gttcagcgtt
     4201 atccttgcga cagccctcgt cgtcgaccgt cgcgaagcgc tcgagtttga ggcttggcag
     4261 cgcgccgacg atcgccgcag gcaaaaagac gcgatgcgca ttgaagcaca cgcggttgcg
     4321 cccgaccggc gtcgacagat agaggcgccc ccctggcgca agcactcgcg caagctcgct
     4381 cagcccggcg cgccagccgt cgggatcaat acggtcgccg taacggccaa gcccgatatg
     4441 ctcaatgacg tgcagtgacg acagcgactg caccgatccg tccgcgaacg ggaggcgggt
     4501 gatgtcggcg gcaacggaat cgagattcgg caaccgcgcc agcagcggac gaaaatcgac
     4561 gaacaacgtc gggacgaagg ccgacaggac gccggtcgcg accacgcttg atccgacgtc
     4621 gacgtgcatc gccggcctgg cgccgttaag ttcacgcgca agccaggcgg cctgataaaa
     4681 atagtgcgga tcgaacggcg tatggccggc gcgatcggtc aggcatggcc gcaggtcggc
     4741 gaagcgcgca gtgtccttgc cggccagccc gcgaaacctg agccaatccg caaaaaaccc
     4801 gggcaggcga acgaccgacg caagctggcg cggatcgacg aacgggagca cccagctgcg
     4861 tccgaaatcc ctgagcatca gttggagggg ttccccgcgg gccagtacgc agcacaacgc
     4921 aggtccctga tcgggtgccc ttccaggtcc ctgcggggtc tgatgcgcgg cgccagccac
     4981 gcccgcagcc gcccggcatt gagatgaaac tgccgcttgg cgacagcggc cattgcccca
     5041 tcagcagcgc tcttcatttc gcggtggcgt acccgctcgc gctctttcgc gtagccggat
     5101 gcgtccgacg acttttgccc cgcgtgcctg cggaaggcgc tgaacgcgaa cggcacatgg
     5161 cgggcgcgcc ctgcccgcgc gatgcgcaga aaaagatcgt agtccgcggc caggtgcagt
     5221 ttccggtcga tcccgccggc gcgcatatag atgtcgcgtg tccagaagca ggcatcctgc
     5281 aacggcgtcc acccgccatg cagcatcgcg gatgccagat cgttgacgta accgcggctg
     5341 tagcggatga cgcggctctg ctcgtcgatg tagaagtcat cgccgaaaat catgtcggca
     5401 tcgcccgcgg cgaagagctc gcgcaccttt gccagcgtgc cgtcgagcag aaggtcgcct
     5461 gcgttgatcc agcacagaac gtcgccgctc gcgcgtttga atcccttgcc aattgcgtcc
     5521 gcatatccct cgtcgcgctc gctgatccaa tgcgtgatgc ttgcttcgtg cttgcggatc
     5581 acgtccacgc tgccgtcggt cgatccgccg tcgacgatga tgtattcgtc gccgggcgca
     5641 aggttgcggc gcacggacga aatcgtctcg ccgagataac cggccatatt gtagttcggg
     5701 gaaacgacgg agattcgcac ggctgtttcc gggacctcac acctggcctg cccgccagca
     5761 ccgcaacatg ggctcgaggt tcgtgaagat gtccatggct ccggaaacgt actcgagcga
     5821 cgtccagcgc cgcgcccgcg caagaccttt cgatacaagc gcggcgcgca gagccgcgtc
     5881 cgaatggata cgcgcgagac cgtccgcaat gtcatccggc ctggcagggt cgacgaacag
     5941 cgccgccccc ccgagttgtt cctccgcacc gggaattcgc gtggccacca ccgggcagcc
     6001 aagcgcgacg gcctcaagcg gcggcagatt ttccgggccg caccaggaca cataggccag
     6061 cccgatcgca ttccggtaga ggtgaaccag atcgtcgcgc gacacaaagc cgagataatt
     6121 cagccgatcg gcaacgccgg cttccttggc caccgaccac acatgatcgc gattgccttt
     6181 gtccgatccg acaaaagcaa aatcgacatc gagtccgtgc cgcgacacga gctcggccag
     6241 cgccagaacg agattgacgt ggttcttgtg cgcccagaat tgcgccggat agagaaagta
     6301 cggccgcctg atcttcagat gtgcgatatc ctgcggggcg ggtgtccccg gcccggcgtc
     6361 gagcgcaaaa cggggcgtcg gatgtggaag cag
//
LOCUS       DASSRL010000430         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_133762, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000430 DASSRL010000000
VERSION     DASSRL010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133762"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFL49_08735"
     CDS             <1..291
                     /locus_tag="VFL49_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HET7912104.1"
                     /translation="LTVNSILVQEAGLSAAEVDGRVVVLSLEAGSYFDLNRVASEIWD
                     MLATPRSVAEILQKLSQHHNVDREIMTRDVMSFLQSLVTQHLARFVAVEDAR"
     gene            288..701
                     /locus_tag="VFL49_08740"
     CDS             288..701
                     /locus_tag="VFL49_08740"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HET7912105.1"
                     /translation="MKLPTRRRRYLREAFVTFVFARLAVRFVAPNRIFAWADRPLRYT
                     SRFSGDEIGWINWALETVAAKSRANDLCLPRALAAHAMLRRRGIASRLCLAVARERRE
                     LAGHAWIEVDGHTIIGDTGGDRFTRIAEFGVPRTV"
     gene            714..>2040
                     /locus_tag="VFL49_08745"
     CDS             714..>2040
                     /locus_tag="VFL49_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET7912106.1"
                     /translation="MSAVAGIIRFDGRSVRRQDLERVANALHQYGPDRSGVVVQDNVG
                     LVHALMRMTPEDRYDHQPGRGAAETMITADLRIDNRDELLDRLAVNQRSAAEWSDARL
                     LLFAWERFGDKIFSMLRGPFAVAIWDSRKRSLTLARDHLGLNVLMWHRDSRSFAFATM
                     PSGLFAFDNVRRELCEEKVADFLVLNHADHVGTIYRNVFRIPPAHVMRVAPDGSVEQR
                     RYWALDEIAPVRLKSDAAYAEGLRDQLDRAVRRQMRSLHPVGALLSGGLDSSSVAALA
                     ARAFAEKTRILPAFTGVPRQGFDGPVPDGSYADERPYVEAIKDRLGNVDVHYVHNSEC
                     DDFAGLERFFIALNGPVRNPSNFGWTTAVLERARQQNCRVLLGGLHGNSTISWDGWSQ
                     SVSHLLRGRLLLAYRQWQLYYRSTPFSRSAAFRRLFIDPLVPLPFGNWIG"
BASE COUNT          375 a          621 c          622 g          422 t
ORIGIN      
        1 ctcacggtca acagcatcct cgtgcaggag gcaggcctgt cggctgccga ggtcgacggt
       61 cgcgttgtcg ttctaagtct ggaagcgggt tcgtattttg acctcaacag ggttgcgagt
      121 gaaatctggg atatgctcgc aacgccacgc agcgtcgcgg aaatcctgca aaagctttcg
      181 caacatcaca acgtggaccg cgaaatcatg acgcgcgacg tcatgagttt tttgcaatct
      241 ttggtcacgc agcatcttgc ccggttcgtc gccgtcgagg atgcccgatg aaattgccga
      301 ccagacggcg ccggtatcta cgcgaagctt ttgtgacgtt cgtctttgca cggcttgcag
      361 tccgtttcgt cgctcccaac cggattttcg cctgggctga ccgcccgctg cggtacacga
      421 gtcggttctc cggcgacgaa atcggatgga tcaactgggc tctcgaaacg gtcgcggcaa
      481 aatcgcgggc aaatgatctg tgtctgcccc gcgcgcttgc ggcgcacgcc atgttgcgcc
      541 ggcgcggcat cgccagcaga ctctgtctcg cggttgctcg cgagcggcgg gagctggctg
      601 gtcatgcctg gatcgaggtc gacggccaca ccatcatcgg tgacacgggt ggtgaccggt
      661 tcacgcgcat tgccgagttt ggagtaccga gaaccgttta ggcatttttg tccatgagtg
      721 cagttgccgg cataattcgt ttcgacggca ggagcgtccg acggcaggac ctcgagcgcg
      781 tagcgaatgc gcttcatcaa tacgggcctg acaggtccgg agttgtcgtg caggacaacg
      841 tcggactcgt tcacgccctg atgagaatga cgcccgagga tcggtacgat catcaaccgg
      901 gccggggtgc cgcggaaacg atgatcaccg ccgacctgcg catcgacaat cgtgacgaat
      961 tgctggatcg ccttgcggtc aatcagcgct ctgccgccga atggtccgac gcacgcctgc
     1021 tgctcttcgc ctgggagaga ttcggcgaca aaattttttc aatgctgcgc ggaccttttg
     1081 cggtcgccat atgggacagc cgcaaaagat cgttgacact cgcacgcgac catctgggcc
     1141 tgaacgtact gatgtggcac cgggacagcc gctcgtttgc cttcgccaca atgcccagcg
     1201 gacttttcgc gttcgacaac gtgcgacgag agctttgcga ggaaaaagtt gctgattttc
     1261 tcgtgctcaa ccatgccgat cacgtcggca ccatctatcg caatgtcttt cgcattccgc
     1321 cggcgcatgt gatgcgcgtc gcgccggacg gctcggtcga gcagcggcgc tactgggcgc
     1381 ttgacgaaat agcccccgtc agactgaagt cggacgccgc gtatgccgaa gggctgcgcg
     1441 atcagcttga ccgcgccgtt cggcgccaga tgcgcagcct gcatccggta ggcgcgcttc
     1501 tgagcggcgg cctcgactcg tcatctgtgg cggcactcgc ggcacgcgcc ttcgcagaga
     1561 agaccaggat attgcccgcg tttaccggcg ttccccgtca aggtttcgac gggccggtgc
     1621 cggacggctc ctatgcggat gaaaggccgt atgtcgaagc gatcaaagac aggctcggca
     1681 atgtcgatgt ccactacgtg cacaatagcg agtgcgacga ctttgccggg cttgaacgct
     1741 tcttcattgc cctgaacggg ccggtgcgga atccgagcaa cttcggctgg acgaccgctg
     1801 ttctcgagcg cgcgcggcag caaaattgcc gcgtattgct cggcggcctc cacggaaact
     1861 ccacgataag ctgggacggc tggtcgcaga gcgtctccca cctgctgcgg gggaggctcc
     1921 tgctcgccta tcgccaatgg caactctact accgcagcac gccattttcg cggtcggccg
     1981 cattcagaag actttttatc gacccccttg tcccattgcc gttcggcaac tggatcggcc
//
LOCUS       DASSRL010000431         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1148642, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000431 DASSRL010000000
VERSION     DASSRL010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148642"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFL49_08750"
     CDS             <1..467
                     /locus_tag="VFL49_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7912107.1"
                     /translation="WDAIIAINLSSSFHTIRAAMPGMKARKWGRIINIASAHGLVASV
                     QKVAYVSAKHGLVGLTKVVALEAANAGVTCNAICPGWVLTALIQQQIDARAKASDKSV
                     RDAEIALLSEKQPMLQFTKPESVGALVVFLCSDAAATITGSAYPIDGGWVAQ"
     gene            478..1566
                     /locus_tag="VFL49_08755"
     CDS             478..1566
                     /locus_tag="VFL49_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7912108.1"
                     /translation="MIELLARAFGGGKSEKPKAGKRINLALQGGGALGAFTWGVLDHL
                     LEDGRLTIEGISGASAGAVNAIILADGLARGGPEEARKRLADFWLAASLGGNLPDLQR
                     RAVDRLFSFWPIEDTPIGLWMQALTRYLSPYDFNPLNINPLKDLVGRFVDFEAVRAAK
                     DLQLFVSATNVRTGELHVFRREQMTADMIVASACLPHLFRAVEIDGESYWDGGYVGNP
                     ALYPLIGATETENLLLVQINPLQRKQVPKTQNEILARINEITFNSSLMSELRGLSFVG
                     QLIDEGKLPRGTGAGQYRRINFHRIALTGSAQTLDTASRLNTDYDFFEMLRTNGRRAA
                     RRFLDAHYDDIGVRSTLEFKTTTGSASA"
     gene            1580..2128
                     /locus_tag="VFL49_08760"
     CDS             1580..2128
                     /locus_tag="VFL49_08760"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HET7912109.1"
                     /translation="MAELSAEIGQTVLDIVVADITTLDVDAIVNAANRSLLGGGGVDG
                     AIHRAAGPDLLAECRTLAGCETGSAKITHGYRLKARHVIHAVGPVWSGGDKDEERLLA
                     GCYRTALDLAAANMLRSIAYPAISTGIFRFPPDLAARIAVGTLASEIAARPRGMARVI
                     FCCFSPDSAEHHKDAFAGLGLV"
     gene            complement(2134..2931)
                     /locus_tag="VFL49_08765"
     CDS             complement(2134..2931)
                     /locus_tag="VFL49_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7912110.1"
                     /translation="MSDDIHEVYAVCYATHARKRSENYIFGDPHDEMTSIAYYVWVIN
                     GPHGTFICDTGFDEAAAKERARKITHSVGEGLLALGVAPDKVDHVIATHMHWDHAGNY
                     DLFPNARYHIQDTEMAYASGRCMCHQMLRIPFSESDVLAMVRKVFAYRVEFHDGVEEL
                     APGITVHKVGGHSKGLQCVRVKTKRGYVVVASDSCHLYSHLDEGRVFPITYSVGDTLE
                     GYKTLKKLASSRNHIVPGHDPEVLKLYPAAKAGLEGWIVRLDVEPKV"
     gene            complement(2928..3824)
                     /locus_tag="VFL49_08770"
     CDS             complement(2928..3824)
                     /locus_tag="VFL49_08770"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7912111.1"
                     /translation="MDIGFIGLGNMGQPMARRLIEAGHKLVVYDTRNDAAAPLVELGA
                     QVASSPSDVADRVETVMASLPSLQISEKVATGEGGVIHGKRIKRFVDLSTTGSQVAAK
                     IAAALAKKNIVQIDSPVSGGVGGAKKGTLAVMVSGPKAEIDLVKDVLSVFGRIFVIGE
                     KPGMAQTMKLANNFLSATAMAATSEAVAMGVKAGLDPAIMIDVINAGSGRTTASDQKF
                     PQAILSRTFDYGFATALMLKDVRLCFDEAKQLSAPNAVMRAVLDQWETTNAEYGGDSD
                     FTAIVKMIEKRAGVVVGSEKKS"
     gene            4028..4459
                     /locus_tag="VFL49_08775"
     CDS             4028..4459
                     /locus_tag="VFL49_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7912112.1"
                     /translation="MDVARLMKTYADQRARQFGISRAQWAVLMRIDRNEGLKQSELAD
                     MLDLQPITLTRLLDRLSENGLIERRADPNDRRANRLYLKPAAKPLLGRLANLGAGMME
                     TVLDGISSTSIERMLKELSAVKDNLRSAIASNANQQQVANG"
BASE COUNT          894 a         1424 c         1311 g          883 t
ORIGIN      
        1 aatgggacgc gattattgcg atcaacctct catcatcatt tcacactatt cgtgccgcca
       61 tgcccggcat gaaggcgcga aaatgggggc gcattatcaa cattgcgtcc gcgcatggtc
      121 tggtggccag tgtacagaaa gtggcctatg tctccgccaa gcacggactc gttgggctca
      181 ccaaggtcgt cgcgttagaa gctgcgaacg ccggcgtgac ttgcaacgcc atctgccccg
      241 gatgggtgct gacagcatta atccagcagc agatcgacgc gcgagctaaa gcctcggaca
      301 agagcgtgcg cgacgcggaa atagcgctgc tatcggagaa acaaccgatg ctccaattca
      361 cgaagccgga aagcgtcgga gcgctcgttg tcttcctatg cagcgacgcg gcggcgacga
      421 tcaccggctc cgcctatccc atcgatggtg gctgggtagc tcagtgacaa gcaggcaatg
      481 atcgaactcc tcgcgcgcgc attcggcggc ggcaaatccg agaagccgaa agccggcaaa
      541 cgcatcaatc tggcattgca gggcggcggc gcgctgggcg catttacctg gggagtgctc
      601 gatcatctgc tcgaggatgg gcggctgacc attgagggaa tttccggcgc atcggcaggc
      661 gcggtaaacg ccatcatatt ggccgacggc ctggcccgcg gcggaccgga agaagcacgc
      721 aagcgtctcg ctgacttctg gctcgccgcc agtctcggcg gcaacctgcc cgaccttcag
      781 cgccgcgccg tcgaccggct gttttcattc tggccaatcg aggatacgcc gatcggcctg
      841 tggatgcagg cactgacccg ttatctgtcg ccctacgact tcaatccgct caacatcaat
      901 ccattgaagg acctggtcgg ccgtttcgtc gatttcgagg cggtccgggc ggccaaggat
      961 ctgcaactgt tcgtatccgc aaccaacgtg agaaccggcg agctgcatgt gttcagacgc
     1021 gagcaaatga ccgccgacat gatcgttgcg tcggcatgcc tgccgcatct tttccgtgcc
     1081 gttgagatcg atggcgagtc gtactgggac ggcggttatg tcggaaatcc ggcgctctat
     1141 ccactgatcg gcgcgaccga gactgaaaat cttctgctgg tccagatcaa tccgttgcaa
     1201 cgcaagcagg tgccgaagac gcagaacgag atcctggcgc ggattaacga gattaccttc
     1261 aactcctcgc tgatgtcgga gctgcgcggc cttagtttcg tcggccagct gatcgacgaa
     1321 ggcaagctgc cgcgcgggac cggcgccggt caatatcgca ggatcaattt tcatcgcatc
     1381 gccctcaccg gatcggcgca gacgctcgat acggccagcc ggctcaacac cgactatgat
     1441 ttcttcgaaa tgctgcgcac caacggccgc cgcgccgcgc gccgctttct cgacgcgcac
     1501 tacgatgaca tcggcgtgcg ttccaccctg gagtttaaga ccacgaccgg ttctgccagc
     1561 gcgtgatatg ctcgcgcaca tggcagagct ctccgccgaa atcggccaga cggtgctcga
     1621 catcgttgtc gccgacatca cgacgttgga tgtcgatgca attgtgaatg cggccaaccg
     1681 ctcactgctc ggcggcggcg gcgtggatgg cgccatccac cgggcggcag ggccggactt
     1741 gctggcggaa tgccgcacgc tggccggctg cgaaaccggc tcggccaaga tcacgcacgg
     1801 ctaccgtctc aaggcccggc acgtgatcca cgccgtcggt cccgtatgga gcggcggcga
     1861 caaagacgaa gaaaggttgc tggcgggctg ctatcgcacg gcgctggacc ttgccgccgc
     1921 caacatgctg cgatcaatcg cctatcccgc aatttcgacc ggcatcttcc gcttcccgcc
     1981 cgaccttgcc gcacgcattg cggtcgggac gctcgcatcc gaaatcgccg cgcgtccgcg
     2041 tggcatggcg cgggtgatct tctgctgttt ctcacccgac agcgcggagc atcataagga
     2101 cgcatttgca ggcttgggac tggtgtgatc ccgctagacc ttcggttcca cgtccagcct
     2161 tacgatccag ccctccagtc ctgctttcgc cgccgggtag agcttcagca cttccggatc
     2221 gtgacctggc acaatgtgat ttcgcgagct cgcgagcttc ttgagtgtct tgtagccctc
     2281 gagtgtgtca ccgacgctgt aggtgatcgg aaagacccgg ccctcatcga gatgcgaata
     2341 gaggtggcag ctgtccgagg cgacgaccac atagccgcgc ttcgtcttca cccgcacgca
     2401 ctgcaagcct ttggaatgac cgccgacctt gtgcacggtg attccaggcg ccagctcctc
     2461 gacgccgtcg tgaaattcca cgcgataggc gaagactttc ctgaccattg ccagcacgtc
     2521 actttcactg aacgggatgc gcagcatctg gtgacacatg cagcggccgc tcgcgtaggc
     2581 catctcggta tcctggatgt ggtaccgggc gttgggaaat agatcgtagt tgccggcatg
     2641 gtcccaatgc atgtgtgttg cgatgacgtg gtcgaccttg tcgggcgcaa cgccaagcgc
     2701 cagcaagcct tcgccgactg aatgggtaat cttgcgcgcg cgctcctttg ccgccgcttc
     2761 atcgaagccg gtgtcacaga tgaaagtgcc gtgcgggcca ttgataaccc agacgtaata
     2821 ggcaatcgac gtcatctcgt cgtgcggatc gccaaagata taattctccg agcgcttgcg
     2881 ggcgtgggtc gcgtagcaga cggcatagac ctcgtggata tcgtcgctca tgatttcttc
     2941 tccgagccga cgacgacgcc ggcgcgtttc tcgatcatct tgacgatcgc cgtaaagtcg
     3001 ctgtcgccgc cgtattccgc gttggttgtc tcccactgat cgagcaccgc gcgcatcacg
     3061 gcattggggg cgctcaactg cttcgcttca tcgaagcaaa gccgtacatc cttgagcatc
     3121 agcgcggtgg caaatccata gtcaaaggtg cgcgacagaa ttgcctgcgg aaatttctgg
     3181 tccgatgcgg tggtgcggcc ggatcccgcg ttgatgacat cgatcataat cgccggatcg
     3241 agcccggcct tgacacccat agccaccgcc tccgacgtcg ccgccatcgc ggtcgccgaa
     3301 aggaagttgt tggcgagctt catggtttgc gccatccccg gcttctcgcc gatgacgaaa
     3361 atcctgccga acacggagag cacgtccttt accagatcga tttccgcttt cggccccgaa
     3421 accatcacgg caagcgtgcc tttctttgca ccgccgacac ccccgctcac cggagagtcg
     3481 atctgtacga tgtttttctt cgccagagcc gccgcaattt ttgccgcaac ctgcgagcct
     3541 gtggtcgaca aatcgacgaa acgcttgatg cgtttgccgt gaatgacgcc gccctcaccg
     3601 gtcgcgactt tttcggaaat ctggagcgac gggaggctcg ccatgaccgt ctcgacgcgg
     3661 tcagcgacat cgctcggcga tgacgcaacc tgagcgccga gctctaccaa aggagccgcc
     3721 gcgtcatttc gcgtgtcgta gacgacgagc ttgtgaccgg cctcgatgag ccggcgcgcc
     3781 atcggctgtc ccatgttgcc gaggccgata aatccgatgt ccatgaactg aattcctgtc
     3841 aggcgacctg tggggatcgc gactttgccc gacgtccgtg cccccgtcac ccgcgcaaga
     3901 cgcgcggggc agacccgacc aaatgacata ttgtgcttat tataagcttg cttattatct
     3961 gcccatccca acaagagcag atcccatggc accgcagccc ctgatacgtg aattcgcctt
     4021 tactatcatg gacgtagccc gtctgatgaa gacctacgcc gatcagcggg cgcgccagtt
     4081 tgggatctcc cgcgcgcaat gggctgtgct gatgcggatt gatcgcaacg aaggcctcaa
     4141 acagtcggaa ctcgctgaca tgctcgacct gcagccgatc acgctcactc gtctgctcga
     4201 caggctgtct gagaacgggc tgatcgaacg gcgtgccgac cccaacgacc ggcgcgccaa
     4261 ccggctctat ctgaagccgg ccgcaaaacc cctgctcgga cgcctggcca atctcggcgc
     4321 cggcatgatg gagaccgttc tcgacggcat ctcttcgacg tcgatcgaac gcatgctcaa
     4381 ggagctcagc gcggtaaaag acaacctgcg ctcggcgatc gccagcaatg ccaatcagca
     4441 acaagtcgcc aacggataaa cgatgactga aaaagcattg aagatcgtgc cggcaccgga
     4501 agccaagacc tc
//
LOCUS       DASSRL010000432         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1341242, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000432 DASSRL010000000
VERSION     DASSRL010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341242"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFL49_08780"
     CDS             complement(<1..838)
                     /locus_tag="VFL49_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912113.1"
                     /translation="MSHVLPAHTPRRNRGNFWSRTWAVLVKEFIQLKRDRVSFAMIIM
                     VPLIQLLLFGYAINTNPRHLPTAVLLQENSDLGRSILAALRNTDYFHVTERPRSEAEL
                     DEMLAAGKILFGIEIPRNFERSVRRGDKPAMLIVADATDPVAASAAVGALGQVVQTAL
                     SHDRGLPQSGGPPFEIRTHQRYNPAGLTQLNIVPGLVGTILTMTMLIFTALSVTREIE
                     RGTMENLLSMPITPVEIMLGKIIPYILVGFVQASMIIGIGVVLFGVPIVGNLFVLAAL
                     STL"
     gene            complement(835..1773)
                     /locus_tag="VFL49_08785"
     CDS             complement(835..1773)
                     /locus_tag="VFL49_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912114.1"
                     /translation="MNEADIVIDVHGLTKKFDGRAVVRNLSMQVRRGTIYGFLGPNGS
                     GKTTTIRMLCGLLTPDEGTGTCLGYDIRTETDKIKLQVGYMTQRFSLYQDLSVRENLE
                     FIGRIYGIANPAEAARDAIKRLGLSGRENQLAGELSGGWKQRLALGACTLPSPKLLLL
                     DEPTAGVDPKARREFWNEIHALAAEGLTVLVSTHYMDEAERCHEIAYIAYGKLLAHGT
                     VEEVIESSHLTTYIVSSHDGEGLNALAEELNDTPGIDMVAPFGTSLHVSGRDADRLET
                     AIAPYRKRKEFEWQRSEPSLEDVFIDLMGKATDNFQ"
     gene            complement(1770..2537)
                     /locus_tag="VFL49_08790"
     CDS             complement(1770..2537)
                     /locus_tag="VFL49_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7912115.1"
                     /translation="MNRTLAGLTLVFATLALAGCNNNNDRTLQGWVEAELVFVSPDEQ
                     GRVETLKVREGDHVKKGDLLFTVDDDLQKADVVVRNTAVVNAQQAFDRAKELLKSAAG
                     TQKTYDDAEAALRQAKANLEWAQTRLARRNAHSPADGTIEQIYYRPGETVSAGRPVVA
                     LLPPGNLKIRFFAPQAVLPELKYGDVVGISCDGCDKDLTAKISFIARSAEFTPPVIYS
                     QEERAKLVFLIEARPEHPEKFRVGQPVTVTLPQGPSK"
     gene            complement(2534..3142)
                     /locus_tag="VFL49_08795"
     CDS             complement(2534..3142)
                     /locus_tag="VFL49_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7912116.1"
                     /translation="MPAIAGRKARSVERREAILTAALDEFSSRGFEAARLEDVARRAG
                     VAKGTIYLYFRDKESLFQELIRTMLTPLVGTIEAMGQADVPLAMLADRFADLFVSQVY
                     ETRRKDVVRLMISEGRRFPKLAEFYYREVLSRIIASVRSLLRRAAARGEVPESLAEFP
                     QIIAAPGLIAIVWNGLFERFEPLDVRKMMKTHLELLFSGRVP"
BASE COUNT          601 a         1019 c          993 g          633 t
ORIGIN      
        1 aaagcgtgct cagtgccgca agcacaaaca ggttgccgac aatggggact ccgaacaaga
       61 cgacgcctat gccgataatc atcgaggcct gcacgaagcc aaccaagata tagggaatga
      121 tcttgccgag catgatctcg accggggtga tcggcattga caggaggttt tccatcgtgc
      181 cccgctcgat ttcacgcgtt accgagagcg cggtgaaaat cagcatcgtc atggtcagaa
      241 tggtgccgac cagcccgggc acgatgttga gctgcgtcag tcccgccggg ttgtagcgct
      301 ggtgcgtcct gatctcgaac ggcgggccgc ccgactgcgg cagcccgcga tcatgggata
      361 gcgcggtttg cacgacctgc ccgagcgcac ccacggccgc gcttgccgcc accggatcgg
      421 tggcatcggc aacgatcagc atagccggct tgtcgccgcg ccggaccgac cgttcgaaat
      481 tacgcgggat ctcgataccg aacaggatct tgcccgccgc gagcatctcg tcgagctcgg
      541 cttcgctgcg cggccgctcg gtcacgtgga aatagtcggt atttcgcagc gccgccagaa
      601 tcgaccggcc caggtcgctg ttctcctgca gcaggacggc ggtcggcaaa tgacgcggat
      661 tggtattgat ggcgtagccg aacagcagca gctgaatgag cgggaccatg atgatcatcg
      721 caaacgaaac gcggtcgcgc ttgagctgga tgaattcctt caccagcacc gcccaggtcc
      781 tgctccagaa attgccgcgg tttcgccgcg gcgtgtgcgc tggcaacacg tggctcattg
      841 gaaattgtcc gtggctttgc ccatcaggtc gatgaagaca tcctcgagcg aaggctccga
      901 gcgctgccat tcgaattctt tgcgctttcg gtaaggcgcg atcgctgtct cgagcctgtc
      961 ggcatcgcgc cccgacacat gcaggctcgt gccgaagggc gccaccatgt cgatgccggg
     1021 cgtatcgttg agctcctcgg cgagcgcgtt gagcccctcg ccgtcgtgcg agctgacgat
     1081 gtaggtcgtc aggtgcgagg actcgatgac ctcttcgacc gtaccatgcg ccaacagctt
     1141 accgtaggcg atgtacgcga tctcgtgaca acgctcagct tcgtccatgt agtgggtcga
     1201 gaccagcacg gtcagacctt cggcggcgag cgcgtgaatt tcgttccaga actcgcgccg
     1261 cgctttcggg tcgacacctg ctgtcggctc atcaagcaaa agcaatttcg ggcttggcag
     1321 cgtgcaggcg ccgagcgcca gccgttgttt ccagccgccc gacagttcgc cggcgagttg
     1381 gttctcacgg ccggacagac ccaaccgctt gattgcatcg cgagcggcct cagcggggtt
     1441 cgcgatgcca tagatgcggc caatgaactc aaggttctcg cgcaccgaca ggtcctgata
     1501 caggctgaag cgctgcgtca tgtatccgac ctgcagcttg atcttgtcgg tctcggtgcg
     1561 gatgtcgtag ccgaggcacg tgcccgtgcc ctcatcaggt gtcagcagac cgcacagcat
     1621 gcggatcgtg gtcgtcttgc cggagccgtt cgggcccagg aagccgtaga tcgtcccgcg
     1681 cctgacctgc atcgaaaggt ttcgcaccac cgcacggccg tcgaactttt ttgtcaagcc
     1741 gtgcacatcg atgacgatgt cggcctcgtt cattttgaag gtccttgcgg caacgtgacg
     1801 gtcacgggct ggccgacacg gaatttctcc ggatgctccg gccgcgcctc gatcaggaat
     1861 acgagcttgg cgcgttcctc ctggctgtag atcaccggcg gggtgaattc ggcgctgcgc
     1921 gcgatgaagc tgatcttcgc agtcagatcc ttgtcgcaac cgtcgcagga tatgcccacc
     1981 acatcgccat atttgagttc aggcagcacc gcctgcggcg cgaaaaacct gatcttcaga
     2041 ttgcccggag gcagcaaggc cacgactggc cggccagccg acacggtttc accgggccgg
     2101 taataaatct gttcgatggt gccatcggca ggactgtgag cgttacggcg ggcgagcctg
     2161 gtctgcgccc attccagatt ggctttggcc tggcgcagcg cggcctcggc atcgtcgtag
     2221 gtcttctggg tgccggccgc cgacttcagc aattccttgg cacggtcgaa agcctgttgc
     2281 gcattcacga cggcggtgtt ccggacaacg acatccgcct tctgcaggtc gtcgtcgacc
     2341 gtgaagagca gatcgccttt cttgacgtga tcgccctcgc gaactttcag cgtttcgacc
     2401 cgcccctgct cgtcgggcga aacaaaaacc agctcagctt cgacccagcc ctgcagggta
     2461 cggtcattgt tgttgttgca ccccgcgagc gcaagcgtcg cgaaaacaag agtcagtccg
     2521 gcgagagtcc tgttcatggc actctccccg agaagagcag ttcaagatgc gtcttcatca
     2581 tcttgcgaac gtcgagcggt tcgaaacgtt cgaacaaacc gttccagacg atggcgatga
     2641 gacccggcgc ggcaatgatt tgcggaaatt ccgcgaggct ttccggcact tcgccgcgcg
     2701 cggcagcccg gcgtaacagc gaccgcacgc ttgcaatgat acgtgacagg acttcgcggt
     2761 aatagaattc ggcgagcttc ggaaagcggc ggccttccga aatcatgagg cgcacgacgt
     2821 ctttccgccg cgtctcatag acctggctga cgaacaggtc ggcaaagcga tcggccaaca
     2881 tcgcgagcgg cacatcggcc tgccccattg cctcgatcgt cccgaccagg ggcgtcagca
     2941 ttgtccggat caattcctgg aacaggctct ctttgtcccg gaagtagaga tagatggtgc
     3001 ccttggcgac gccggcgcgg cgcgccacgt cctcaagacg cgccgcctcg aaaccgcggc
     3061 tggaaaattc gtcgagtgcg gcggtcagaa ttgcttcgcg gcgttcgacg ctgcgcgcct
     3121 tgcgtccggc gatggccggc actgcggcgg cgcgcgccgc ttcggttgca cgctttcgcc
     3181 ggccggcacg gcccgctcct gcttgtcgaa acccgggtcc acgacgcata ctgcaccttt
     3241 atgact
//
LOCUS       DASSRL010000433         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_851389, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000433 DASSRL010000000
VERSION     DASSRL010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851389"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFL49_08800"
     CDS             complement(<1..952)
                     /locus_tag="VFL49_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7912117.1"
                     /translation="MSVVELSLVLMLLLFVILGSGVWIAIALAVCGFAAVLTKVATPA
                     GQVLATSMWTASNSWDLTALPMFIWMGEILYRTKLSDDMFEGLAPWLQKLPGRLLHVN
                     VVGCAIFAAVSGSSAATCATIGRIALPELLKRGYDERMAVGTLAGSGTLGLLIPPSII
                     MIVYATATDQSIARLFIAGVIPGIVLAGLFMGFIVVWALFNQTRMPPPDPRVPFRERV
                     HASRRLIPVMLLILGVIGSIYGGLASATEAAVVGVFLALALSWWSGTLTKQGLIDSLL
                     GATRTSCMIAFILAGAAYLTAAMGFTGIPSALAEWISGYNM"
     gene            complement(957..1514)
                     /locus_tag="VFL49_08805"
     CDS             complement(957..1514)
                     /locus_tag="VFL49_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET7912118.1"
                     /translation="MRRFLDTLYRASLWASALCLVAIALMVGAQLAARMIDGTLALLH
                     LPRTEFVILSLNEICGYLLAAASFLALAGTLKAGAHIRVTMILAALSERIRYFIELWA
                     FGFAAAASAYMTGHLANFAWVSFLFNEVSAGVIRVPLVYPQAAMALGALVLTTALVDE
                     FFIVLHGGRPSFRIAEDAITLGKEG"
     gene            complement(1599..2573)
                     /locus_tag="VFL49_08810"
     CDS             complement(1599..2573)
                     /locus_tag="VFL49_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET7912119.1"
                     /translation="MKSFIRVCAGLLLAGGIATVASAQTKWDMPTPYPDGNFHTRNII
                     QFAADVDKATNGSLKIAVHSAGSLIKHPEIKRSVRQGTAPIGEILESLSSNEAAVYGL
                     DSVPFLATGYDASRKLYAAQKPYLEKQLASEGLILLYSVPWPPQGLYAKREIKSVDDL
                     KGLKFRTYNAMIGRIAALAGAVPTQIEVPDLPTAFATGRVDVMITSASTGVDTKAQDY
                     LTHYIDTQAWLPRNIVFVNKAAFDKLSAAEKKAVTDAAKAAEERGWKLSTEEMAIKTK
                     ALKDAGIKVVPPSPELKAGLAKIGATITSEWEKSAGPDGAAMLAAYRK"
BASE COUNT          604 a          846 c          878 g          495 t
ORIGIN      
        1 gcatattgta tccgctgatc cactcggcga gcgcgctcgg tattccggtg aaccccatgg
       61 ccgcagtcag gtaagcagcg ccggcgagga taaaggcaat catgcaactg gttcgcgttg
      121 cgcccagcag cgagtcgatc aagccttgct ttgtcaaagt gcccgaccac caggacagcg
      181 ccagcgccag gaacacgccg accaccgcgg cttcagtagc ggatgcaagt ccgccataga
      241 tcgagccgat gacgccgagg atcagcagca tcacgggaat gagtcgccgc gatgcatgga
      301 cgcgctcccg aaacggtacc cgcggatcag gcggcggcat gcgcgtttga ttgaacagtg
      361 cccacaccac gatgaagccc atgaaaagcc cggcgagtac gattccggga atgacaccgg
      421 caatgaacag ccgtgcgatc gattgatcgg tcgcggtggc gtagacaatc atgatgatgg
      481 atggcgggat cagaagcccg agcgtgcccg aaccggcgag tgtgccgacg gccatgcgtt
      541 cgtcgtagcc gcgcttgagc aattccggca gcgcaatccg gccgatggtc gcgcaggtcg
      601 ccgccgagga acccgacacg gcggcgaaaa tcgcgcagcc cacgacgttg acgtgcaaca
      661 ggcgccccgg cagtttttgc agccatggcg ccaggccttc gaacatgtcg tcggacagct
      721 tcgtgcgata gaggatctct cccatccaga taaacatcgg cagcgccgtc agatcccacg
      781 agttcgacgc cgtccacatg gaggtcgcaa gtacctgacc cgccggcgtt gcaactttcg
      841 tgagcaccgc ggcgaagccg caaacggcca gtgcaatcgc gatccagacg ccggacccaa
      901 ggatgacgaa caggagcagc atcaacacga gtgaaagctc gacaaccgac atcgggctag
      961 ccttctttcc cgagcgtgat ggcatcctcg gcgatgcgga aactcggacg gccgccgtgc
     1021 aggacaataa aaaactcatc gaccagcgcc gttgtcagga ccagcgcgcc aagagccatc
     1081 gcggcttgcg gatagaccag cgggacgcgg atgacaccgg ccgatacctc gttgaaaaga
     1141 aacgaaaccc aggcgaaatt ggcgagatgg ccggtcatgt aggcggatgc cgccgcggcg
     1201 aagccgaagg cccaaagctc aataaaatag cggatacgtt cactgagcgc cgcgaggatc
     1261 atcgtcacgc gaatatgggc gcctgccttc agcgtgcccg caagggcaag gaaactggca
     1321 gctgcaagca gatagccgca aatttcattg agcgagagaa taacgaattc ggtgcgcggc
     1381 agatgcagca gcgcaagcgt tccatcgatc atgcgcgcag caagctgcgc accgaccatc
     1441 agcgcgatcg cgaccagaca tagggcagac gcccaaagag atgcgcgata aagcgtgtcg
     1501 aggaagcggc gcatgctcag gactttcccc cacccggaaa ccctctcccc atatggaaga
     1561 gggttagtcc atgcctgcag tcaggccgcg aatgacaatc atttcctata ggccgcgagc
     1621 atcgccgcac cgtccggtcc ggcggacttc tcccattcgg acgtgatggt cgcaccgatc
     1681 ttggccagcc cggctttgag ttcgggactt ggcggcacga ccttgatgcc ggcgtctttc
     1741 agcgccttgg tcttgatagc catttcttca gtggagagct tccatccgcg ctcttcggca
     1801 gccttggccg catcagtgac agccttcttt tcggccgccg acaatttgtc gaacgccgcc
     1861 ttgttcacga atacgatgtt acgcggcagc caggcctgcg tgtcgatgta gtgtgtcaga
     1921 taatcctgcg ccttggtgtc gacgccggtt gatgccgagg taatcatcac atcgacgcgg
     1981 ccggtcgcga aggcggtcgg cagatcaggt acctcgatct gcgtcggcac cgcgcccgca
     2041 agggcggcga tgcggccgat catggcgttg taagtgcgga atttgagacc cttaagatca
     2101 tcgacagact tgatttcgcg cttggcgtag aggccttgcg gcggccatgg caccgaatag
     2161 aggagaatca gtccttcgga agcgagttgc ttttcgagat acggcttctg cgccgcgtaa
     2221 agcttgcgcg aagcgtcgta accggtggca aggaaaggca ccgagtcgag cccgtagacc
     2281 gccgcttcgt tcgacgacag cgactcgagt atctcgccga tcggcgccgt gccctggcgc
     2341 acgctgcgtt tgatctcggg atgcttgatc agcgaaccgg cggaatgaac cgcgatcttg
     2401 agagagccgt tggtcgcctt gtccacgtca gcagcgaact ggatgatgtt gcgggtgtgg
     2461 aaattgccgt caggatacgg cgtgggcatg tcccatttgg tttgtgctga agcgactgtc
     2521 gcaatgccgc cggcaagcaa caggcccgcg cacacgcgga tgaaactttt cataggtccc
     2581 tccaccattc ttcggcgttg tgtaagcatg gggccaatgc cgtggcggtg gcaagggcat
     2641 gccggcaagg tttgctcagg cggggtcgac gccgttcgcc ttcagcgtcg gcgcagcggc
     2701 gggtgcggag aggaaacgaa tcagcgcctg cgcggcatcg cgttccttcg tggaagcgct
     2761 caagccggcg gcaaacccga tcttggtctg cagttcctcc ggaatcggcc cgaccagatc
     2821 aac
//
LOCUS       DASSRL010000434         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1341267, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000434 DASSRL010000000
VERSION     DASSRL010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341267"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFL49_08815"
     CDS             <1..1504
                     /locus_tag="VFL49_08815"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET7912120.1"
                     /translation="PIYQVNFNLELAGGAKPLRWTAYTGRLGARGGNWRATIDTSGAL
                     MVATTRSLAPGEGLTVVAELPSHAIDPPSESQLLWYRLHDNRAWIFGGIGLLVVLGYY
                     FIAWEAVGRDPKGGAIIPLFHPPAGISPALANYIHDWGFGRDKWRAFTAAALSLAVRG
                     LLRFDESGGTLTLKSAGKDAPAKLPAGERTVLTWVNNKGGTAVISDAHGASIAKAGDN
                     FTESIEKENRNRFFRRNLGYVIAGLAMTGAVVIGIVMFGGLQDQDVSILAGIAVGGFV
                     AGILSVQLLQTFFRRISLASLVRGAMSVAFLAIFISISQSILKEVTPRTLIDSFPAAW
                     SYVESYPFSFALVGAFTMVNGLFFYLLRAPTALGRPIMDQLAGLRLYLQTAESDRLNL
                     SAPEITAQRFEALLPYAVALDVEKPWSDAFAAALRRAHPDDQDPMSSYHSSWSSGRWS
                     SSNFGSAVSSSISGVSSALASSVPVSSGSSGFGGGGGSGGGGGGGGGGGW"
     gene            1578..>2722
                     /gene="glyS"
                     /locus_tag="VFL49_08820"
     CDS             1578..>2722
                     /gene="glyS"
                     /locus_tag="VFL49_08820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7912121.1"
                     /translation="MPDLLLEFLSEEIPARMQARAAADLRKLVTDRLVAAGLVYEGAK
                     AFATPRRLALSVHGIPARQPDIKEEKKGPRVGAPENAIAGFLKAAGLKSIQDARVQPD
                     KKGDFYVAMIEKEGRAAIEVIAEMIPDIVRTFPWPKSMRWGAGKLNWVRPLHSIVATF
                     GPETEEPDIVRFGVDGLAAGDETRGHRFMAPAPFRVRHFDDYVAKLEKAKVVLDPARR
                     ADAILADAKNLAFAQGFELVEDQGLLAEVAGLVEWPVTLMGSFDRAFLAIPDEVVRAT
                     IRNNQKCFVLRDPQTAKLVNKFILVANEEAADGGEAIVAGNERVIRARLSDAKFFYET
                     DLRMRLEDRLPKFQQIVFHQKLGTQAERIARIVSLAGKLAPLVGADVA"
BASE COUNT          480 a          839 c          874 g          529 t
ORIGIN      
        1 tccgatttat caggtgaact tcaatctcga gctcgccggt ggagcgaaac ctctgcgctg
       61 gaccgcctat accggccgcc tgggtgcgcg cgggggcaac tggcgggcca cgatcgatac
      121 gtccggcgct ttgatggtcg cgaccacgcg gtcgctcgca cccggtgagg gactgacggt
      181 tgtcgcggag ctcccatccc acgcaatcga cccgccgagc gagtcgcagc tgctttggta
      241 ccgtctgcac gacaaccgcg cctggatttt cggcggcatc ggcctgctcg tcgtgctcgg
      301 ttactatttc atcgcgtggg aagcagtcgg acgcgatccc aagggtggcg ctatcattcc
      361 gctatttcac ccgccggcag ggatctctcc cgcgctcgcc aattacattc acgactgggg
      421 tttcggacgc gacaagtggc gcgccttcac ggcggccgcc ctgtcgctcg cggtgcgtgg
      481 acttttgcgc ttcgacgaat ccggcggcac gctgacgctg aagtccgccg gcaaagatgc
      541 acccgccaag ctgccggcag gcgagagaac ggtcctcacc tgggtgaaca acaagggcgg
      601 cacggcggtc atcagcgacg ctcatggtgc ctcaatcgcc aaagcaggcg acaattttac
      661 cgagagtatc gagaaagaaa atcgcaaccg cttcttccgc cgcaatctcg gctacgtcat
      721 agccggactg gcgatgaccg gcgcggtcgt gatcggcatt gtcatgttcg gcggcttgca
      781 ggatcaggac gtctcaattc ttgccggcat tgcggtcggc ggatttgtgg ccggaatatt
      841 gagcgtccaa cttctgcaga cgtttttccg gcgcatcagt ttagcctcgc ttgttcgtgg
      901 cgccatgtcg gtcgcatttc tcgcaatttt catttcgatc tcgcagtcga ttttgaagga
      961 ggtgactccg agaactttga tcgattcgtt ccccgcagcc tggtcctacg tcgaaagcta
     1021 cccattttcg ttcgcgctcg tcggcgcatt caccatggtg aacggcctgt ttttctatct
     1081 gctgcgagcg ccgacggcgc tcggccgtcc gatcatggac cagctcgccg ggctgcggct
     1141 ctatcttcag acagctgaat ccgatcgcct caacttgagc gcgccggaaa tcaccgcgca
     1201 gcgctttgaa gccttgctgc cctatgcggt tgcgctcgac gtggagaagc cctggtccga
     1261 cgcctttgcc gccgcgcttc gccgcgcgca ccccgacgat caggacccga tgagcagcta
     1321 ccattcgagc tggagctcag gcaggtggtc gagcagcaat ttcggcagcg ccgtgtcgtc
     1381 gtcgatttcc ggtgtgagca gcgcactcgc gagttcggtc ccggtttcct ccgggtcctc
     1441 cggtttcggc ggaggcggcg ggtccggcgg cggcggggga ggcggcggtg gaggaggctg
     1501 gtaaaacctg caggcgcgcg tgacagccgc gggccgcctt gctaggcaag cccgcgcgaa
     1561 caccgaattc ctccaatatg cccgacctgc tgctcgaatt tttgtctgaa gagatcccgg
     1621 cgcgcatgca ggcgcgcgcg gctgccgacc tgcgcaagct ggtgaccgac cgcctggttg
     1681 cggcggggct cgtctatgag ggtgccaaag cctttgcgac gccgcggcga cttgccctgt
     1741 cggtgcacgg cattcctgcg cgccagccgg acatcaagga agaaaaaaag gggccccgtg
     1801 tcggcgcgcc ggaaaatgca attgccggat ttctgaaggc ggccgggctc aagtcgattc
     1861 aggatgccag agtacagccc gacaagaaag gcgatttcta tgtcgcgatg atcgagaagg
     1921 agggcagggc ggcgatcgag gtcatcgccg agatgatccc cgacatcgtg cgaacgttcc
     1981 cgtggccgaa gtcgatgcgt tggggtgcgg gaaaactcaa ctgggtgcgg ccgttgcact
     2041 cgatcgtcgc cactttcggg cctgaaacgg aagagccgga catcgtgcgt ttcggcgtcg
     2101 atggcctggc ggccggcgac gagacgcggg gccaccgttt catggcgccg gcgccgttca
     2161 gggtgcgcca tttcgacgac tacgtggcaa agcttgaaaa ggccaaggtc gtgctcgatc
     2221 ccgcgcgccg cgccgacgcg atcctggcag atgcgaaaaa tctcgccttc gcgcagggct
     2281 tcgagcttgt cgaagatcag gggctattgg cggaagtcgc cggccttgtc gagtggccgg
     2341 tgacgctgat gggaagtttt gacagagcgt ttctggcgat tcccgacgag gtcgtccgcg
     2401 ccaccatccg caacaaccag aaatgcttcg tgctgcgcga tccgcagacg gcgaaactcg
     2461 tcaataaatt cattctcgtt gcaaacgagg aagcggcgga cggcggcgag gcaatcgtcg
     2521 ccggcaacga gcgggtgatc cgcgcccggt tgtccgacgc gaagttcttc tacgagaccg
     2581 acctcaggat gcgcctcgag gatcgtctgc cgaaattcca gcagatcgtc tttcatcaaa
     2641 aactcggcac gcaggccgag cgcatcgccc gcatcgtgtc gctcgccgga aaattggcgc
     2701 cgctggtcgg cgccgacgtg gc
//
LOCUS       DASSRL010000435         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_379121, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000435 DASSRL010000000
VERSION     DASSRL010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379121"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFL49_08825"
     CDS             complement(<1..383)
                     /locus_tag="VFL49_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7912122.1"
                     /translation="MNRSSAAIHIVDDDASFRSAMGELLSACGYRVSLYETAKELLES
                     SLDSVPACILLDVQMAGLSGPQLQEKLSSLGCRLPIVFISGHGDIPITVRTIKAGAVD
                     FRTQPVTKEKLLETVERALTQHCAI"
     gene            540..881
                     /locus_tag="VFL49_08830"
     CDS             540..881
                     /locus_tag="VFL49_08830"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HET7912123.1"
                     /translation="MRNSLVTTITYSALILSLTDTASAQNLPWCAIMDNDGTTQCNYY
                     TQQQCLQTLSGIGGECIRNPAGNAPNNGQRPPLFAPFSGNSQGLLPLQSEDPGPPPGL
                     DGTAIPPPPNN"
     gene            909..1334
                     /locus_tag="VFL49_08835"
     CDS             909..1334
                     /locus_tag="VFL49_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912124.1"
                     /translation="MSDCRIAAGLSLLVYFLFSSVHAQQTIVVPDNLFTQAGFVVKYA
                     TTPEKAAILRSLPADKLVTRRKDGKLYYVYADATGCNCAYVGTPEAFVRYQNGASVGG
                     NAPNGQSWSSIQTIDSDVSAPYIPADIGDSMESILQPDF"
     gene            complement(1709..2461)
                     /locus_tag="VFL49_08840"
     CDS             complement(1709..2461)
                     /locus_tag="VFL49_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HET7912125.1"
                     /translation="MTAYVVLPRAWTHYEHQKRLAGLMMVTRTSQDIPGDPINVGLVG
                     TRDDVLCAMHAAGWYPADPITFRSSVEIVGSVVLRRPYQDAPVSNLYYDGRRQDLAFE
                     KPVGKSADQRHHVRFWEVLTQGEENRPVWLGSATFDRDVGLSRYTGQVTHHIAPDIDA
                     ERDGLTDDLKNSKVVTAIYEVSGVGPTFIGRNGEGDRYFTDGEVRISRLVSGCGTKAE
                     TTVELKNPPLIDLKNETWKALAGLLQAGAVAK"
     gene            complement(2611..>3982)
                     /locus_tag="VFL49_08845"
     CDS             complement(2611..>3982)
                     /locus_tag="VFL49_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7912126.1"
                     /translation="NRSGMTTVAFQGQGGGLPAAEWTLGSVLKTAGYRTFFAGKWHLG
                     ESDYALPNAQGYDEMRYCGLYHLNAYTYGDPTWFPDMDPELRAMFNRVTKGAMSGNAG
                     QPAKEEFKINGQYVNTPVINGKEGVVGIPFFDEYVEKASLDYLDRNARSTQPFFMSIN
                     FMKVHQPNMPAPEFEHKSLSKSKYADSVVENDTRIGRIMDKVRALGLDKNTYVFWTTD
                     NGAWQDVYPDAGYTPFRGTKGTVREGGNRVPALAWGPRIKAGSKNSDIVGGLDFMATF
                     ASLAGVKLPENDRAGQPIIFDSYDMSPILFGTGKSARKEWFYFTENELTPGAARVGNY
                     KAIFNLRGDDGAKTGGLAVDSNLGWKGAESYVATVPQVFDLWQDPQERYDIFMNNYTE
                     RTWTMVTISDSIKKLMATYVKYPPRKLQSESYSGPITLTNYQRFQWVRDQLAKEGVSI
                     AMPTGN"
BASE COUNT          811 a         1211 c         1116 g          844 t
ORIGIN      
        1 tgaatcgcgc agtgctgagt taaagcacgt tcgacggtct ccaacagctt ctctttggtg
       61 accggttggg tacggaagtc tactgcaccg gccttgatcg tccgcacggt tatcgggatg
      121 tcgccgtgcc cgcttataaa aacaattgga agccggcatc ccaaactgga aagcttttcc
      181 tgcagttgcg ggccgctcag cccggccatt tgcacgtcga gcaagatgca ggccggcacg
      241 ctgtccagtg atgactcgag caattccttt gcggtttcat agagcgaaac tctgtatccg
      301 cacgcgctca gcagttctcc cattgctgat cgaaaggatg catcgtcatc gacgatgtga
      361 atggctgccg aagacctgtt cataggagca cgcgccgccc gtttttgccg gcaaaattcg
      421 gcacttgggg gctattgttc cgaaaaccgg tttccggccc aattgcccct aagggaaata
      481 gcgtcagaca gccattcggc ccacgattac gcctgcgact gcccttgccc ggagccgtca
      541 tgcggaattc attagtaact acaataactt acagcgcgct tattttgtca ctcaccgata
      601 cggcgagcgc gcagaacctt ccctggtgcg ccatcatgga caacgacggc acgacacagt
      661 gcaactacta cacccagcag cagtgcctac agacgctgag cggtatcggt ggcgaatgta
      721 tccgcaatcc tgccggaaac gcaccaaaca acgggcaacg accaccgctt tttgcgccgt
      781 tttcgggaaa ttcgcagggg ctgctgccac tccagtcgga ggacccggga ccaccaccag
      841 gactcgacgg gaccgcgatt cctccgccac caaacaacta acgtaaacgt cgccgccggg
      901 ggcttacaat gtcggactgc cgcatcgccg caggattgtc actgctggtt tacttccttt
      961 tttcatccgt acatgcgcaa caaaccatcg tcgttccgga caacctcttc acccaggcgg
     1021 gctttgtcgt gaagtacgcg acgacgccgg agaaggcagc gattctccgg tcattgccgg
     1081 cggacaagct cgtgacgcga cggaaggacg ggaagctcta ttacgtttat gccgatgcta
     1141 ccggctgcaa ttgcgcctat gtgggaacgc ccgaagcctt tgtgcgatac cagaacgggg
     1201 caagcgtggg cggcaatgcc cccaacggtc agtcgtggag ttcgatacaa acaatcgact
     1261 ccgatgtcag cgctccgtac ataccggcgg acatcggcga cagcatggag agcattcttc
     1321 aaccggactt ttaggattcg aatcgttccg cgactctcaa tggcagagat cgatacaaaa
     1381 tgcgccggac gcgatacagg cgacatagga tccgaatggg cagaaaggac gccgctgtat
     1441 aagcgggtca cgcaccgtaa cgtacccgcc cgaatatttg gcccgcgatt cctgccggaa
     1501 tggcgagtac gagcgaggcg acccgcaacg atcaacgcag tagtcggcca ccgcgatctt
     1561 tgaccgaaac ttatacggtt gcagatttga gagcggcatt gagtgcgctg ccgttgcggc
     1621 aatgcagatt gcgacagaag taataattgc tttcatggcg tcactcccgc agtacttgga
     1681 tcgaagcgtc gacctctgac cccacacctc atttggcaac tgctcccgcc tgcaatagtc
     1741 cggcaagcgc cttccacgtt tcatttttca gatcgatcaa cggcggattt ttcaattcga
     1801 cggttgtttc agccttcgtg ccgcagccgg aaaccagacg tgagattctc acttcgccgt
     1861 cagtgaaata ccggtcgccc tccccgttgc ggcctatgaa ggtcggcccg acgcccgaga
     1921 cctcgtagat cgccgtcacc accttggaat ttttgagatc gtcggtaagc ccgtcgcgtt
     1981 cggcgtcgat atccggcgca atatgatggg tgacttgccc ggtatagcgg ctaagtccga
     2041 cgtcgcgatc aaacgttgcc gaacccagcc ataccggccg gttttcttcg ccctgcgtca
     2101 ggacctccca gaagcggacg tggtggcgct gatccgcgct tttgccgacg ggcttttcga
     2161 aggcgagatc ctgtcgcctg ccgtcgtagt aaaggttgct caccggcgcg tcctggtacg
     2221 gcctgcgcag aacaacgctg cccacaatct cgacgctcga gcggaacgtg atgggatcgg
     2281 cagggtacca gccggccgca tgcatggcac aaagcacgtc atctctcgtg ccgaccaagc
     2341 cgacgttgat cggatcaccc ggaatatcct gcgaggtgcg cgtcaccatc atcagcccag
     2401 caagcctttt ttgatgctcg tagtgcgtcc aggcgcgcgg caacacaaca taagcggtca
     2461 ttccatagac gagcaccccg gctcccagcg cggcgagcac aattctcgcc ttagaccgcc
     2521 gggaaccggc atcaacccgg tttcgccacc acttcataaa tgacgctgct gccgctgtag
     2581 aagaaccgac aggctttcat ggaggccgca tcagttgcct gtgggcatcg caatgctcac
     2641 gccctccttt gcaagctggt cgcgcaccca ctggaatctt tgatagttcg taagcgtgat
     2701 cggtcccgaa tagctttcgc tctgcaattt gcgcggcggg tatttcacgt aggtcgccat
     2761 cagcttcttg atcgagtcgc tgatcgtcac catcgtccag gtgcgctcgg tgtagttgtt
     2821 catgaagatg tcgtagcgct cctgcgggtc ctgccagaga tcgaagactt gcggcactgt
     2881 ggcgacgtag gattccgcgc ccttccagcc gaggttcgag tcaaccgcga gtccgccggt
     2941 ctttgcacca tcatcgccgc gcaaattgaa gatggccttg tagttgccga cgcgggcggc
     3001 gccgggcgtc agctcgttct cggtgaaata gaaccattcc ttgcgtgccg attttccggt
     3061 gccaaacagg atcggcgaca tatcgtaact gtcgaagatg atcggctgac cggcgcgatc
     3121 gttctccggc agcttcactc ccgcgagcga cgcaaaggtg gccatgaaat ccagcccgcc
     3181 gacgatatcg gagttcttgg acccggcttt tatccgcggg ccccaggcca gcgcgggaac
     3241 gcggttgccg ccctcgcgca ccgtgccttt ggtgccgcgg aacggcgtgt agccggcgtc
     3301 aggatagacg tcctgccagg cgccattgtc cgtcgtccag aagacatagg tgttcttgtc
     3361 gagaccaagt gcgcggacct tgtccatgat cctgccgatg cgcgtgtcgt tctccaccac
     3421 cgagtccgcg tatttgctct tcgaaagcga tttatgctca aactcgggag ccggcatgtt
     3481 cggctgatgc accttcatga aattgatgct catgaagaac ggctgtgtcg agcgcgcatt
     3541 gcgatcgaga tagtcgagcg atgccttttc gacgtactcg tcgaaaaacg gaatgccgac
     3601 gacaccttcc ttgccgttga taaccggcgt attcacgtac tgcccgttga tcttgaattc
     3661 ctctttcgcc ggctgtcctg cgttgccgga cattgcgccc ttggtgacgc ggttgaacat
     3721 ggcgcgcagc tcaggatcca tgtccggaaa ccaggtcggg tcaccgtagg tgtaggcgtt
     3781 gaggtgatag aggccgcaat atctcatttc gtcatagccc tgcgcgttcg gcagcgcata
     3841 gtcggattcg ccgaggtgcc atttgccggc gaaaaacgtc cgatagcccg ccgtcttcag
     3901 caccgagcca agcgtccact cggcggcagg caggccgccg ccttgaccct ggaatgccac
     3961 cgtcgtcatg ccgctgcggt tc
//
LOCUS       DASSRL010000436         8760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_694310, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000436 DASSRL010000000
VERSION     DASSRL010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694310"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFL49_08850"
     CDS             <1..348
                     /locus_tag="VFL49_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET7912127.1"
                     /translation="LAGYKRATNIIRIEEKKDRREYTGAPDARRYQLAEEKALAEAID
                     AARKEASAAIGMEDFAAAMRAIAKLRPRVDAFFDKVTVNVDDKALRENRLKLLNEIRE
                     ATRAVADFSKIEG"
     gene            433..576
                     /locus_tag="VFL49_08855"
     CDS             433..576
                     /locus_tag="VFL49_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HET7912128.1"
                     /translation="MVIGIARLQPIIALIAGILILVIPRLLNVIVAVYLIIAGLLGLG
                     LFR"
     gene            651..3428
                     /gene="ppdK"
                     /locus_tag="VFL49_08860"
     CDS             651..3428
                     /gene="ppdK"
                     /locus_tag="VFL49_08860"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET7912129.1"
                     /translation="MAKRKRRRVSAKSKKKALRKPARRKAKPAAAPARGKWVYSFGDG
                     RAEGKASMRNLLGGKGAGLAEMASLGLPVPPGFTITTDVCTYYYTHGKQYPKDLRAQA
                     EGALAQVGRITGKTFGDRSNPLLVSVRSGARASMPGMMDTVLNLGLNDETVEALAKKS
                     GDRRFAYDSYRRFITMYSDVVLGVGHEHFEELLDHHKERQGYSLDTDLAADDWAELVM
                     RYKKRVKDELGHDFPQDPHEQLWGAIGAVFGSWMNQRAITYRKLHSIPESWGTAVNVQ
                     AMVFGNMGDTSATGVAFTRNPSTGERKLYGEFLINAQGEDVVAGIRTPQEISEEARKE
                     AGSDKPSMEYTLPKAYAELKRIYNVLERHYRDMQDLEFTVEQGKLWMLQTRSGKRTAK
                     AALRIAVELANEGLISRNDAVLRIDPLTLDQLLHPTIDPRAHRRVIATGLPASPGAAS
                     GEIVFSPDEAAQLKSDGRKVVLVRVETSPEDIHGMHAAEGILTTRGGMTSHAAVVARG
                     MGKPCVSGAGSLRVDYAGGSMTAGGQTFKKGDYITVDGSTGQVLAGKVEMIEPQLSGE
                     FATLIGWADKVRRLGVRANADTPTDAKAAVRFGAEGIGLCRTEHMFFDEDRIQAVREM
                     ILADDEKTRRAAIAKLLPMQRADFVELFEIMAGRPVTIRLLDPPLHEFLPHGEAEIAE
                     VAAAMGTDAAKLAARANELSEFNPMLGFRGCRIAIAYPEIAEMQARAIFEAAVDAAKR
                     TGKPVVPEVMVPLIATRAEFDLVKSHIDAMAQAVAKEKDVRVNYQVGTMIELPRASLM
                     AGEIAETAEFFSFGTNDLTQTTFGISRDDAASFLGIYTARGILPVDPFVSIDPQGVGE
                     LVKIGVERGRKVRPKLKVGICGEHGGDPASVTFCHEAKLDYVSCSPFRVPIARLAAAQ
                     AALGKAVASQA"
     gene            3440..4204
                     /locus_tag="VFL49_08865"
     CDS             3440..4204
                     /locus_tag="VFL49_08865"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7912130.1"
                     /translation="MAIFDLKNRVAFITGGNGGIGLGMARGLAQAGASIVIAGRNRTK
                     AESALSELRSLGAPAEFVQLDVLDEKSCGQAIRIAVKRFGRLDILVNNAGTTVRKQPE
                     DLSAQDWHLVMDTNLTGAFLCSQAAYPHMVRAGGGKIINIGSMMSVFASSYAAPYAAS
                     KAGIVQLAKSMAVAWAKDNIQVNAILPGWIDTDLTQGARKQVQGLHEKVLARTPAQRW
                     GVPGDFAGVAVFLASPASNFITGVAIAVDGGYSAQV"
     gene            complement(4218..5567)
                     /locus_tag="VFL49_08870"
     CDS             complement(4218..5567)
                     /locus_tag="VFL49_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HET7912131.1"
                     /translation="MFVSLLPSTIVGQQVIAGLCFAGFIVACLAYRRLQNQNRRISTA
                     LNNMSQGLNMFDAQGRIILLNSRYLEMYKLDPAVVKPGCTLKRLIEYRKETGLFSGDV
                     DSYVQKILDAMSQSKSQSHYVEASDGRIVLAKNEPLPEGGWVSTHEDVTEQRQAEQER
                     AAIRSQEQRRTTIDSAITSFRPAVEKLLSSVSSSATAMRSTARALFDSSEHTSQRAES
                     AVQAFNEASANVETAAVAADELSRSIGEISRQLTQTSNVVGLATNEARATDGEIAGLA
                     DGAQKIGDVVKLIRNIAGQTNLLALNATIEAARAGEAGKGFAVVASEVKSLAVQTAKA
                     TEDIASHILAVQESTSGAVEAIRQIATRMQEINGNTTAVAASVEQQNLATGEISRSVT
                     SAAEGTNQVVAVLGEVAGAATQTRSSAEIVRDASEAVEHAVGDLRLEVEGFLGKVAV"
     gene            5855..7264
                     /locus_tag="VFL49_08875"
     CDS             5855..7264
                     /locus_tag="VFL49_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HET7912132.1"
                     /translation="MRRQPGAAAFGIGLALLATFSDTIGFQDLGALLARQSAVARRWQ
                     QHLIASPFGTIHAATFSLPRPIGTGIPHPPIYALANFDPADITGSIGSQLLGDPTAPL
                     QFPKPNRKAKRDALVARAREPMPPLPPALAIEPVPEAEVDASLKKEDNTRFDPYAQYE
                     FVPAPDQRVTTPDVDIPYADIPPANLTPTAPQRATGKEAKQLFFGVNSIAVGGGKTAP
                     KIVPWAPGEAPVVLASHGDPDIKQSALSPALPREEGEGGESVASKGEVTGVDQRPRSP
                     AERLALTGTARAKAEKCLANAVYFEARGESVRGQIAVAQVVMNRVFSPFYPNDVCGVV
                     YQNSGRHLACQFTFACDGIPDIVTEPDAWERARRIARDMLDGKLWMPEVSKSTHYHAY
                     WVHPDWVNEMKKVYKLGVHTFYRPRAWGNGEDEPTWGDPAETEKEAAKEEAQGPVSHS
                     KEWLKSEEGQASLRQSARK"
     gene            7407..8657
                     /locus_tag="VFL49_08880"
     CDS             7407..8657
                     /locus_tag="VFL49_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912133.1"
                     /translation="MSVQSTTGKTIAVGAWRTPLVIITCGCLIGAIGFGPRAALGLFL
                     TPMSSAYSWGRDVFALALAIQVLIWGAATPFAGAIADRFGTVRVLSVGALFYCAGLVL
                     MAHSTTPGMLNFSAGILTGLGIAGTSFTLVLAAFSKLMPAEWRGFAFGVGTAAGSVGQ
                     FLFSPLAVGLIDFIGWQQALITLGIFTLLMIPCAFGVRTPPMDHAPAPGGAQKQSLVQ
                     ALSEAFGHRSYVLLVLGFFTCGFQLLFITVHLPSYLVDRGLSVDAGAWTIGVIGLFNI
                     FGSIAAGWLGNKMPMRYLLSVIYLLRSLAVAVFITLPVTAVSAVVFGAAMGLLWLSTV
                     PPTSGLVALMFGTRWMATLFGFAFFSHQVGGFLGVWLGGVLFERTGSYEVIWWLSIAL
                     GIISAIINMPIIEKPVVRAEPVPA"
BASE COUNT         1587 a         2732 c         2741 g         1700 t
ORIGIN      
        1 ctggcgggct acaagcgcgc gaccaacatc attcgcatcg aggagaagaa ggacaggcgc
       61 gaatataccg gcgcgcccga tgccaggcgc taccagctgg cggaagagaa ggcgctcgcc
      121 gaagcaatcg acgccgccag gaaggaagct tctgccgcca tcggcatgga ggatttcgcc
      181 gcggcgatgc gggccatcgc caagcttcgc ccgcgcgtcg atgccttctt cgacaaggtg
      241 acagtcaacg tcgacgacaa ggccttgcgc gagaatcgcc tcaagctgct caacgaaatt
      301 cgcgaggcga cccgtgccgt ggcggatttt tcgaagatcg aaggctagtg ttcagttcgc
      361 gtagggtatt cagggaacta ctgttaccgt ctcgccgttt ctttcgattg ccagccagtt
      421 gaggagctaa gcatggtcat tggtattgcg cgtttgcagc caattatcgc gctcatcgcc
      481 ggtattctca tccttgtcat tccgcggctg ctcaacgtca tcgtcgcggt ctatctcatc
      541 atcgccgggc tgctcggcct cggccttttc cggtagttga aattcggctt gcgcccgcct
      601 gtttggaagt gtttttaagg gccgttgttt tcattcatag ctaatgaccc atggccaaac
      661 ggaaacgtag gcgcgtgagc gcaaaatcca agaagaaggc attacggaag cccgcccggc
      721 gcaaggcgaa gccggcggcc gcgccagcca ggggcaagtg ggtctacagc ttcggagacg
      781 gccgcgccga aggcaaagcc tcgatgcgca acctgctcgg cggcaagggc gccgggctcg
      841 ccgaaatggc aagcctcggc cttccggttc cgccagggtt cacgatcacc accgacgtct
      901 gcacttacta ctacacgcac ggcaagcaat atccgaagga ccttcgcgcg caggccgagg
      961 gggcgcttgc ccaggtcggc cgcatcaccg gcaagacgtt cggcgacagg tcgaacccgc
     1021 tgctggtctc ggtgcgatcc ggcgcacgcg cctcgatgcc tggcatgatg gatacggtgc
     1081 tcaaccttgg gctcaatgac gagaccgtcg aggcgcttgc aaaaaaatcg ggcgaccggc
     1141 gcttcgccta cgacagttac cggcgcttca tcaccatgta ttccgacgtc gtgctcggcg
     1201 tcggccacga gcatttcgag gagttgctcg atcaccacaa agagcgccaa ggatacagtc
     1261 tcgacaccga tctcgccgcc gacgactggg ccgagctggt catgcgctac aagaagcgcg
     1321 tcaaggatga gctgggccac gattttccgc aggatccgca cgagcagcta tggggcgcga
     1381 tcggcgccgt gttcggctct tggatgaacc agcgcgccat cacctaccgc aagctgcaca
     1441 gtattccgga gagctggggc acggccgtca acgtgcaggc catggttttc ggcaacatgg
     1501 gcgacacctc ggcgacggga gttgcgttca cgcgcaatcc gtcgacgggc gaaaggaagc
     1561 tttacggcga gtttctcatc aatgcgcagg gcgaagacgt tgtcgccggc atacgtacgc
     1621 cgcaggaaat cagcgaagag gcgcgcaagg aagcgggctc cgacaagccg tcgatggaat
     1681 acacgctgcc caaggcctat gccgagctca agcgcatcta caatgttctc gagcgtcact
     1741 accgcgacat gcaggatctc gagttcacgg tcgagcaggg caagctctgg atgctgcaga
     1801 cgcgttcggg aaagcgaacg gcgaaggccg cgctgcgtat tgcggtcgaa cttgccaacg
     1861 aggggctgat ttccaggaac gacgcggtgt tgcgcatcga tccgctcacc cttgaccagc
     1921 ttctgcaccc gacgatcgac ccgcgcgcac accggcgcgt catcgcgacg ggcctgccgg
     1981 cctcgcccgg agccgccagc ggcgagatcg ttttctcgcc ggacgaggcg gcgcagctca
     2041 aatccgacgg ccgcaaggtt gttctggtgc gcgtcgagac atcgccggaa gatattcacg
     2101 gcatgcacgc ggctgaaggc atcctcacca cacgcggcgg catgacctcg catgctgccg
     2161 tcgttgcccg cgggatgggc aagccgtgtg tttcgggcgc gggctcgcta cgcgtagact
     2221 atgccggagg ctcgatgacg gccggcggac agacgttcaa gaaaggcgac tacatcaccg
     2281 tcgacggttc aaccggccag gtgctggcgg ggaaggtcga gatgatcgag ccgcagctgt
     2341 ccggcgaatt tgccacactg atcggctggg ccgacaaggt ccgcaggctc ggcgtgcgcg
     2401 ccaacgccga tacaccgacc gacgcgaaag cggcagtgcg tttcggcgcc gagggcattg
     2461 gcctctgccg taccgagcac atgttcttcg atgaggaccg catccaggcg gtgcgcgaga
     2521 tgattctcgc cgacgacgag aaaacgcgcc gcgcagcgat cgccaaactg ctgcccatgc
     2581 agcgcgcaga tttcgtcgaa ttgttcgaga ttatggccgg gcggccggtg accatccggc
     2641 tcctcgatcc gccgctgcat gaattcctgc cgcacggcga ggcggaaatc gcagaagtcg
     2701 ccgccgccat ggggaccgat gcggcgaaac tcgcggcccg cgccaacgag ctctccgaat
     2761 tcaatccgat gctcggcttc cgcggctgcc gcatcgccat cgcgtatccg gagatcgccg
     2821 agatgcaggc acgtgcgatc ttcgaggcag cggtcgatgc cgcgaagcgc accggcaagc
     2881 cggttgtgcc cgaggtcatg gttccgctga ttgccaccag ggcggaattt gacctggtca
     2941 aatcgcacat cgacgccatg gcgcaggcgg tcgccaagga aaaggacgtc agggtcaact
     3001 accaggtcgg tacgatgatc gaactgccac gcgcctcgct gatggcgggc gagatcgccg
     3061 aaaccgccga atttttctcg ttcggcacca acgatcttac gcagacgacc ttcggcattt
     3121 cgcgtgacga cgcggcaagc ttcctcggca tctataccgc gcggggaatt ctgccggtcg
     3181 atccgttcgt gtcgatcgac ccgcaaggcg tcggcgaact tgtgaagatc ggggtcgagc
     3241 gcggccgcaa ggtgcgcccc aagctcaagg tcggcatctg cggcgagcat ggcggcgatc
     3301 ccgcgtcggt cacattctgt cacgaggcca aactcgacta tgtctcgtgc tcgcccttcc
     3361 gcgtgccgat cgcgcgcctt gcggcggcgc aggctgcgct cggcaaagcg gtcgccagtc
     3421 aggcataacg gccgccgtca tggcaatttt cgacctgaag aaccgggtcg ccttcataac
     3481 aggcggcaac ggaggcattg gcctcggtat ggcaagaggt ttggcccagg ccggcgcgtc
     3541 gattgtcatc gccggccgca acaggaccaa agcagaatcg gctctgtcgg agctgcgatc
     3601 actcggcgcg ccggcggaat ttgtgcagct ggatgttctg gacgagaaat cctgcggtca
     3661 ggcaatccgg attgccgtga agcgatttgg ccgtctcgac atcctcgtca acaacgccgg
     3721 aaccacagtc cgaaagcagc cggaggatct ctcggcacag gactggcacc tggtgatgga
     3781 caccaatctc accggcgctt ttctgtgttc gcaggccgcc taccctcaca tggtgcgcgc
     3841 cggcggcggc aagatcatca atatcggatc gatgatgtcg gtgttcgcct cgtcctacgc
     3901 cgcgccttat gccgcaagca aggccggcat cgtgcagctg gcgaaatcaa tggcggtcgc
     3961 ctgggcgaag gacaatatcc aggtcaacgc aatcttgccc ggatggatcg acaccgatct
     4021 cacccaaggc gcacgcaagc aggtccaggg cctgcatgaa aaagtattgg cccgcacgcc
     4081 agcccagcgt tggggcgttc ccggcgattt tgccggagtt gccgtgtttc tggcatcgcc
     4141 tgcttcgaat ttcatcaccg gggtggcgat cgcggtggac ggcggctatt cggcccaggt
     4201 ctgacctgaa acagcggcta aacggccacc ttgccgagga agccttcgac ttcgagccgc
     4261 aggtcgccga ccgcatgctc gaccgcttcc gacgcatcgc gcacgatttc ggccgagctg
     4321 cgcgtctgcg tcgccgcgcc ggcgacctct ccgagcactg cgaccacttg attggtgcct
     4381 tcggccgcgc tggtgacgct gcgggagatt tcccctgttg ccaggttttg ctgctcaacc
     4441 gaagcggcga ctgccgttgt gttgccgttg atctcctgca tgcgcgtggc gatctgccgg
     4501 atcgcctcaa ccgcacccga cgttgattcc tgcacggcga gaatgtgact ggcgatgtcc
     4561 tcagttgcct tcgcggtctg caccgcgagc gattttactt ccgaggcgac aacggcaaag
     4621 cccttgcccg cctcgccggc gcgcgcggcc tcgatggtgg cattgagcgc gagcaagttc
     4681 gtctgcccgg caatgttgcg gatcaacttg accacgtcgc cgatcttctg ggcgccatcg
     4741 gccaggcccg cgatttcgcc gtcggtggcg cgcgcctcat tggtagccaa cccgacgacg
     4801 ttgctggttt gcgtcagttg acggctgatc tcaccgatcg atcgcgacaa ctcgtcggct
     4861 gcaaccgctg cagtctcgac gttggccgac gcttcgttga aggcctgcac cgcgctttcc
     4921 gccctttgcg aggtgtgctc ggatgaatca aacagtgcac gcgcggtcga acgcatggcc
     4981 gtcgcgctgc tgctcacgct tgacaggagc ttttcgacgg ccggacggaa cgaagtgatg
     5041 gccgaatcaa tggtcgtgcg gcgctgctcc tggctgcgga tggcggcgcg ttcctgctcg
     5101 gcctgacgct gctccgtgac gtcttcgtgg gtggacaccc atccgccttc cggcaaaggc
     5161 tcgttcttgg ccagcacgat gcggccgtcg cttgcctcca cgtagtgtga ctggctcttg
     5221 ctttgcgaca tcgcatcgag aattttctgc acgtagctgt cgacgtcacc ggaaaacagt
     5281 ccggtctcct tgcggtattc gatcagcctc ttgagggtgc agccaggttt cacaaccgcg
     5341 ggatcgagct tgtacatctc aagatagcgg ctattgagca aaatgatgcg gccctgggcg
     5401 tcgaacatgt tcaggccctg ggacatgttg ttcaatgccg tagagatccg tcggttctgg
     5461 ttctgcaggc gccgataggc aaggcaggcc acaatgaacc cggcaaagca cagcccggcg
     5521 attacctgtt gacccacgat ggtcgacggc agaagcgaca caaacatgct gatcctagaa
     5581 ttaccttcgg cgatattggg atgcagcgat tgcgatatag ttaattcttc ccatccaggc
     5641 tcaatccggc acgcgcctct gtgggtgcgc tgcagctatg actttctccg ccgttgagaa
     5701 agcatttacg acagcagcgc gtttgcccgc gggattaaca tcttcgcaag gttaatcggt
     5761 tcataagaaa acgtgtcgcg ttgtcgccac ccggcggcgg gcggtggcag gacgttgcgg
     5821 tgtggcgttg ggcggacggg gagcagtttc gcttttgcgt cggcagccgg gagctgcggc
     5881 gttcggcatt ggattggcgc tgctcgcgac gttttccgac acgatcggct ttcaggacct
     5941 tggcgccttg ctggcgcggc aatccgccgt cgccaggcgt tggcagcagc atctcattgc
     6001 ctcccctttc ggcacgatcc acgcggcgac gttcagcctg ccgaggccga tcggcaccgg
     6061 cattccgcat ccgccgatct acgctctcgc caatttcgac ccggccgata taaccggctc
     6121 gatcggcagc caactgctcg gcgatccgac ggcgcccctt cagtttccaa aacccaatcg
     6181 caaggccaag cgcgacgcgc tggttgcgcg tgcacgcgag ccgatgccac cgttgccacc
     6241 ggcgctcgcc atcgagcctg tgcctgaagc cgaggtcgat gcttcgctca agaaagagga
     6301 caacacgcgc tttgacccct atgcgcaata cgaatttgtc ccggctcccg atcagagagt
     6361 tacgacaccc gacgtcgaca ttccttatgc cgatattcca cccgcaaacc tgactccgac
     6421 ggcgccgcaa cgcgcgacgg ggaaggaggc gaagcaactg tttttcggcg tcaactcgat
     6481 tgcagtcgga ggcggaaaga ccgcaccaaa aatcgtgcct tgggcgcccg gcgaagcacc
     6541 ggtcgtgctc gcctcgcacg gagatccgga tatcaagcaa tccgctctgt caccggcatt
     6601 gccgcgcgaa gagggcgaag gcggcgagag cgtcgccagc aaaggcgagg tcaccggggt
     6661 cgaccagcga ccgcgatcgc cggccgaacg gctcgcgctg acgggaactg cgcgcgccaa
     6721 agccgagaag tgtcttgcca acgcggttta cttcgaagca cgcggcgaat ccgttcgcgg
     6781 ccagatcgcg gtggcgcagg ttgtcatgaa ccgcgtgttc tctccgttct atccgaacga
     6841 tgtttgcggg gttgtctacc agaactccgg ccggcacctg gcttgccagt tcaccttcgc
     6901 ctgcgacggc atccccgata tcgtcacgga acccgacgcc tgggagcgtg ccaggcgaat
     6961 cgcgcgcgac atgctcgacg gcaagctatg gatgccggag gtatcgaaat cgacccacta
     7021 tcacgcctat tgggtccacc cggactgggt caacgagatg aagaaagttt ataagcttgg
     7081 cgtgcatacc ttctatcgcc cacgcgcatg gggcaatggc gaggatgagc cgacctgggg
     7141 cgatcctgcg gaaacagaga aagaggctgc gaaagaagaa gcgcaggggc ccgtaagcca
     7201 cagcaaagaa tggcttaaat cggaggaggg acaggcgtcg cttcgccaga gcgcccgtaa
     7261 gtagtgcgtt cggcgaacgt catgtgtcgc gcaaatgcgc gcgatcacga attttcgttt
     7321 tggcggaccc ggatgaacct atatgttccg cgcctgtccg cggcccgatc ctgtggccgc
     7381 ggcgctgcag ttggccggag caattcatgt cagtgcaatc gacgacgggg aaaacgatcg
     7441 cggtcggtgc gtggcgcacg ccgctggtca tcatcacctg tggttgcctg atcggcgcga
     7501 tcggtttcgg cccgcgcgcc gcgctcggct tgttcctcac gccgatgtcg tccgcctaca
     7561 gctggggccg tgacgtgttc gcgctggcgc ttgcgatcca agtcttgatc tggggtgcag
     7621 cgactccgtt cgccggcgcc atcgctgacc gcttcgggac ggtgcgcgtg ctgtcggtcg
     7681 gcgcactgtt ttactgcgcg ggtctcgtgc tgatggccca ttcgaccacg cccggcatgc
     7741 tcaatttctc cgccggcatt ctgaccggcc tcggcattgc aggaacgtct ttcacactcg
     7801 tgctggcggc tttcagtaaa ttgatgccgg cagaatggcg cggcttcgct tttggcgtcg
     7861 gcacagccgc cggttcggtc ggccagttcc tgttctcgcc gctggcggtg gggctcatcg
     7921 actttatcgg ctggcagcag gctctgatca cgcttggcat ttttacactt ctgatgattc
     7981 cctgtgcgtt cggtgtgcgc acaccaccga tggatcatgc gccggcgccg ggtggtgcgc
     8041 agaagcagtc actggtgcag gcgctgtcgg aagctttcgg ccaccgcagc tatgtgctgc
     8101 tcgtgctcgg cttcttcacc tgcggtttcc agcttctttt catcacggtg catctgcctt
     8161 cctatctcgt cgaccgcggc ctctcggtcg atgccggcgc gtggaccatc ggcgtcatcg
     8221 ggctgttcaa catcttcgga tcgatcgccg ccggctggct cggcaacaag atgccgatgc
     8281 gttacctgct ctcggtcatc tacctgctgc gctcgctcgc ggtcgcggtt tttatcacct
     8341 tgcctgtaac ggcagtatcg gcggtcgttt tcggcgcggc gatgggcctc ctctggctgt
     8401 cgacagtgcc accgacttcc ggcctggtag cgctgatgtt cggcacgcgc tggatggcga
     8461 cattgttcgg ctttgccttc ttcagccatc aggtcggcgg ttttctcggc gtatggctcg
     8521 gcggcgtgct gttcgagcgc accggttcat atgaagtgat ctggtggctg tcgatcgcgc
     8581 tcggcattat ttccgcgatc atcaacatgc cgatcatcga gaagccggtc gtccgcgcgg
     8641 agccggttcc cgcttgaacg cttgcgtccg gcctccggcc acgccatgaa tgccagggct
     8701 gaaccggcga ggcgcgtgtg acggcattca aggcagtggt aattgagaaa tcggagaccg
//
LOCUS       DASSRL010000437         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_694331, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000437 DASSRL010000000
VERSION     DASSRL010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694331"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1424)
                     /locus_tag="VFL49_08885"
     CDS             complement(57..1424)
                     /locus_tag="VFL49_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HET7912134.1"
                     /translation="MNLHTKLLEREAAGRPVTVAVIGAGKFGTMFLSQARLTRGMHVV
                     AVADLDVRRARSQLNTAGWPGETYAATSRLDAHKKRATFVTDDADALIADPQIEVIVE
                     ATGVPGAGIRHALTAITNGKHIVMVNVEADAVAGPLLARRAKAAGVVYSLAWGDQPAL
                     ICEQVDWARAAGFKVICAGKGTRYEPHYHKSNPDNVWDILDKYLKISDRKSINAKMFN
                     SFVDGTKSGIEMTAVCNVTGLVPQSDGLGFPPATRFELADICKPSSDGGRLEKAGVTE
                     VTSSVYRDGRDVPHHLALGTYVVFEGDTEYARRCFQEYAMLPDKTGKYAALYRPIHMI
                     GLELGISVASAALRNESTGAPTGFRSDVVATAKRALKAGEVLDGEGGFCVWGKQTPAD
                     ASLDRELLPLGLAHAVKLKRDIAEGQALRWSDVAYDPNDSAVKVRREMEAAFDRRNVG
                     AEAAT"
     gene            complement(1489..2040)
                     /locus_tag="VFL49_08890"
     CDS             complement(1489..2040)
                     /locus_tag="VFL49_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912135.1"
                     /translation="MNTKQALNFRRTALTRAPAFVWLFAVGLVLASALAFAQDAAPAG
                     EKPKEDTGFLATMSRWFSQQSANVNSTFKDARQKVEGLGQEAGAAAKSTVEGAKDAAG
                     AVARIPNVRTVNGHEKCQIAPNGAPDCVAAANSICKAKGFDSGRSLDMTTAEICPPKV
                     WMAGRSTGPECHTETFVSRAFCQ"
BASE COUNT          423 a          707 c          652 g          403 t
ORIGIN      
        1 gcaagatcgg tctcaatcac aaggtcgacg gcgagatcga actcggttga cgcgcgctag
       61 gttgcagcct ctgcgccgac attgcgccgg tcgaaggcgg cctccatctc gcgtcgcact
      121 ttcaccgcgg aatcattcgg atcgtaggca acgtccgacc accgcagggc ctgcccctcc
      181 gcaatgtcgc gcttgagctt gaccgcatgc gcaagaccga gcggcagcaa ttcgcgatcg
      241 agcgatgcat ccgcgggcgt ctgcttgccc cagacgcaga acccgccctc accgtcgagc
      301 acttcgcccg ctttcagcgc gcgctttgcc gttgcgacca cgtcggaacg gaagccggtc
      361 ggcgctcccg tcgactcgtt gcgcagcgcc gcactcgcga cggaaatgcc gagctcgaga
      421 ccgatcatgt ggatcgggcg gtaaagggcg gcgtatttgc cggttttgtc gggcagcatc
      481 gcgtattcct gaaaacaccg ccgcgcatat tctgtgtcgc cctcgaacac gacatatgtg
      541 ccgagcgcaa gatgatgcgg cacgtcacga ccgtcgcggt agaccgacga cgtcacttcg
      601 gtgacgcctg ctttttccag cctgccgccg tcactactgg gcttgcagat gtcggcaagc
      661 tcgaaccgcg tcgccggcgg aaaaccgagc ccgtccgact gcggcaccag cccggtgacg
      721 ttgcagaccg ccgtcatctc gatgccggac ttggtcccgt ccacaaacga gttgaacatc
      781 ttcgcgttaa tcgacttgcg gtcggagatc ttcaggtact tgtcgaggat gtcccacaca
      841 ttgtccggat tggacttgtg atagtgcggc tcgtaacgcg tgcccttgcc cgcgcagata
      901 accttgaagc cggcggcgcg cgcccaatcg acctgctcgc agatcagtgc gggctgatcg
      961 ccccaggcaa gcgaatagac aacgccggcg gccttcgcgc ggcgcgccag caaaggaccg
     1021 gccaccgcat cggcctcgac gttgaccatg acgatgtgct tgccgttggt gatcgccgtg
     1081 agcgcgtgcc ggataccggc accaggcacg ccggttgctt cgacgatgac ttcgatctgc
     1141 ggatcggcga tcagtgcatc ggcatcgtcc gtgacgaatg tggcgcgctt cttgtgcgca
     1201 tctaaccggg aagtagcggc ataagtttcg ccgggccagc ccgccgtatt caattgactc
     1261 cttgcgcgac gaacgtcgag gtcggcaacg gcgacaacgt gcatgccgcg cgtgagacgc
     1321 gcctgtgaaa gaaacatcgt gccgaatttg ccggcgccga tcaccgcaac ggttaccggg
     1381 cggccggccg cctcgcgctc aagaagtttt gtatggagat tcaagaggcg ctccgtcgct
     1441 gacgcgcgac ggaaacacaa tcacggcagc ggcacaagca gtccgatcct actggcagaa
     1501 tgcgcgcgaa acgaacgttt cagtgtggca ctccgggccg gtcgatcgtc ctgccatcca
     1561 gaccttgggc ggacagatct cggctgtcgt catatcgagg ctgcggcctg aatcaaaacc
     1621 tttcgccttg cagatggaat ttgccgcagc gacacagtca ggcgctccgt tcggcgcgat
     1681 ctggcatttc tcgtgaccgt tcacggtacg cacgttcgga atgcgtgcca ccgccccggc
     1741 cgcatccttg gcgccttcga cggtggactt ggcggcggca cccgcctctt ggccgagacc
     1801 ttcaactttc tgacgcgcat ctttgaatgt cgagttcacg ttggcagatt gctgcgaaaa
     1861 ccagcgactc atggttgcaa gaaatcccgt gtcttctttc ggcttttcac ccgcaggcgc
     1921 ggcgtcctgc gcgaacgcta atgccgacgc cagcacaagc ccgaccgcga acagccagac
     1981 gaatgccggc gcgcgtgtca gagcagtccg acgaaaattc aacgcctgct tggtgttcat
     2041 ggccggcaaa aaagggaaat caatcagcct gacggcaccc gcttacccgc tttcggtggc
     2101 gaaaatgagg aaccggctcc gtcagaacaa gtatagcgca gccaccactc cgatgacgag
     2161 aaccagggcg ccgagcagca cccat
//
LOCUS       DASSRL010000438         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1638855, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000438 DASSRL010000000
VERSION     DASSRL010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638855"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1065)
                     /locus_tag="VFL49_08895"
     CDS             complement(298..1065)
                     /locus_tag="VFL49_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET7912136.1"
                     /translation="MDIRQLIELFEIESGTLNSLLRQHAHLRALFSRDFKGVDLNALK
                     LSYLQFLKMKADYVQFTCPAFRAAGEALSGGDEEDRRWSKVFLEYSDGEADEKEGYGH
                     EVWARNDMKVLGASPGLLDASPHASALLYGEYFVNEAAHHPYAILGAKGVLEHLSIIM
                     SDDIADGMLESRIAGAENAVTFFRHHGVLDIEHVREGDRNLERSLKDSGRIRQVFESI
                     CFTSTVYRWMLESYISHDESAANSISRPHALTTATVD"
     gene            complement(1043..1861)
                     /locus_tag="VFL49_08900"
     CDS             complement(1043..1861)
                     /locus_tag="VFL49_08900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HET7912137.1"
                     /translation="MRSEPAAYEIKQLDTLEELIQSYRLRYEVYADLGYLRQANKSKL
                     EIDEYDAWAIPFGAFDVASRTMVGTLRLVTKQPQPDFSRLVQQLLELFADDSLAKQAR
                     APRPHILPSIVSDKIHQALAAFNTENFIVCELSRCVVGRNFRGSGVSRSLMELGLAQA
                     SFGGTALLIGSYLPEHLPMYAKYGYLRLPQTDLEMFNSVGQIAIAGICRTDRLPQPTR
                     SHVDELLRSVRTSVAEHPTETDVAGAFYGAYKARRALGPAQQEWKVYNGHSTTD"
     gene            2366..2674
                     /locus_tag="VFL49_08905"
     CDS             2366..2674
                     /locus_tag="VFL49_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912138.1"
                     /translation="MRNLTAALTLIVAVSLNAPSFARDVSVGKHTAEDVKSICDKVGG
                     KFSQDTTGHYCGTDCHGAPGTDCVVGCKTGQPCIAQVIGGRRPTNLRNALEAPRKAAH
                     "
     gene            2907..>3591
                     /locus_tag="VFL49_08910"
     CDS             2907..>3591
                     /locus_tag="VFL49_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7912139.1"
                     /translation="MARTGMSPALHKGPANKPKRSLDETRAIWLAAFRRVRADTEKRA
                     SHLSAEDQIVQSMADASPAKWHRAHVTWFFEQFLLVPHDSSYRIFDERFPFLFNSYYV
                     AAGPRHARPQRGLITRPNAADVAAYRTHVDAAVERLIAQVPAADADRVFAIVEVGLHH
                     EQQHQELLLTDILHAFAQNPTDPAYDAKWQAPRATQGPRGYVDVPAGVHAIGHDGEGF
                     CFDNETPYHD"
BASE COUNT          747 a         1026 c         1048 g          770 t
ORIGIN      
        1 catggtcatg gtctgtctct gcgtcgtcag tgacagtccg taatttcaac ttctgtcggg
       61 ccttccggag ttcagccaag gttccgtctg gcgagcgagg acaggtttcg ccaagctggt
      121 ctcttgggac aacgatttag ccgtcgaaca ctgcgggaaa ccaaacgata aattcaacaa
      181 cccgattgcg atcttgaacg atattttgcg cagccctcgg cgtcgacggg tgggcttcca
      241 attactcaca actgacgacg aaacggcgag aaaagaatcg tgcacggcca cagcgtttta
      301 gtcgaccgtg gctgtcgtga gggcgtgcgg gcgcgatatg gaatttgcgg cggactcgtc
      361 gtgtgaaata taggattcga gcatccaccg ataaacagtg ctcgtgaaac aaatgctctc
      421 gaacacctgc cggatccttc ccgagtcctt caagcttcgc tccaggttgc gatcgccttc
      481 gcggacgtgt tcgatatcaa gcacaccgtg atggcgaaaa aacgtgacgg cgttctccgc
      541 gccggcgatc cggctttcga gcatgccatc ggcgatatcg tctgacatga tgatggacaa
      601 atgctcgagc actcccttgg ccccaaggat cgcgtaaggg tggtgggcag cttcattgac
      661 gaagtattcg ccgtagagaa gtgcgctggc gtgcggcgac gcgtccaaca ggcccggcga
      721 agctccaagc accttcatat cgttacgcgc ccacacttca tggccgtaac cttccttttc
      781 gtcggcttct ccgtcggagt attcgaggaa gaccttgctc cagcggcgat cttcctcatc
      841 acctccgctc aaagcctcgc cggcggcgcg aaatgcggga cacgtgaatt gcacgtaatc
      901 ggctttcatt ttgagaaatt gcagatagct tagctttaat gcgtttaaat caacgccttt
      961 gaagtcgcgt gaaaacaaag cgcgcaaatg cgcatgctgt cgcaataggc tgttgagtgt
     1021 tccgctttcg atctcgaata gttcaatcag ttgtcgaatg tccattgtaa accttccact
     1081 cctgttgcgc agggcccagc gcccggcgtg ccttgtaagc gccgtagaat gcgcctgcca
     1141 catccgtctc agttgggtgc tcggctacac tcgttctcac agagcggagc aattcatcga
     1201 cgtgagaccg tgttggttga ggcagcctgt cggtgcggca gatgcccgcg attgcgatct
     1261 ggccgacact attgaacatt tcgaggtcgg tctgaggaag cctcaggtat ccgtacttgg
     1321 catacatggg caggtgttca ggcaaatagg agccgatgag gagcgcggtg ccgccgaacg
     1381 atgcttgtgc aagtcccaat tccatcaggc tgcgcgaaac gccagatccg cgaaagttgc
     1441 gaccgacgac gcaacggctg agctcgcaaa cgatgaagtt ttcggtattg aaggcagcga
     1501 gggcctgatg gattttgtcg gagacgatcg aaggaaggat gtgagggcgc ggagcacgcg
     1561 cctgcttcgc caaagagtca tccgcgaata gctccagcag ttgctgaaca aggcgcgaaa
     1621 agtcaggttg cggttgcttt gtcacaaggc gcaacgtgcc taccatcgtg cgcgacgcca
     1681 catcgaaggc gccaaacggg atcgcccacg catcgtactc gtcaatctcg agctttgatt
     1741 tatttgcctg gcgcaggtag ccgaggtcgg cataaacctc gtagcggagc ctgtatgact
     1801 gaatcagctc ttcgagcgtg tcgagctgct tgatctcgta tgctgcgggc tcagacctca
     1861 tgggaatccg gtccatgatg agggtccggc aggcgcggga gccttgggga tcggcgccgc
     1921 attgaggtcg gccgcctgtg acgagccggc aatcgcgagc gcggcgaatg tagtgcctgc
     1981 gagcaattca ttttttataa agcagcctcc gcatcagacc agattgaaaa taaaggttgt
     2041 ggcgggagag gcttgggcgg gcgcgtcgta tgatttgtct aaagcgacaa tgccggggtt
     2101 atttgccgct ttggtgaaga ttttgctttc tgctcaatgc gcgcgtggca aattcatctt
     2161 cccggcgagc acgccggccg accacttgag aggaataaga aactcctcgc ggagaacctc
     2221 tccgggatgc aaaggggcaa tttatcgggc cttccagggt ccatccgaaa cacacccgga
     2281 agtagtcttc tggcctcaag tgctgaaatg gctgtaagct tgaacggatc ttcccaaggg
     2341 gcgacgtacc ttgttggaga ctgtcatgcg caatttgacc gccgccctta ccctgattgt
     2401 cgccgttagc ctcaatgctc cgtcttttgc tcgcgacgta tccgtcggaa agcacaccgc
     2461 cgaggatgtc aaaagtattt gcgacaaagt cggcggcaaa ttttcacagg acacaacggg
     2521 acattattgc gggaccgact gtcatggcgc ccctggcacc gattgcgtcg tcggctgcaa
     2581 gaccggtcag ccatgcatcg cccaggtcat cggtggcagg cgtcccacca atcttcgcaa
     2641 cgccctcgag gcgccgcgaa aggccgccca ctaaacttct ccctttgggg cgacaaaacc
     2701 gatagaagta gcggtttccc gctaccgatt ccggccgata gcaaaaggca atccggtcgt
     2761 tttgaccgcg aacgccgacg cggtttcgtc gcattcatgc gttcaaggtt gctagcacgt
     2821 cctcgcgatg gaacaattgc gtccattatt cgtctttgta cggacatcga ctgccgaagc
     2881 cctttgtata ggcacggagg atttccatgg cgcgaaccgg aatgtcgccg gctctccata
     2941 aagggccggc gaacaagccc aagcgttccc tggacgaaac tcgtgcaatc tggcttgcgg
     3001 ctttccgccg ggtgcgggcg gacacggaaa agcgtgcatc gcatctgtcg gccgaagacc
     3061 agatcgttca gtcgatggct gacgcgagcc cggccaagtg gcaccgggcg cacgtcacgt
     3121 ggtttttcga gcaattcctt ctggtgccgc atgattcctc gtaccggatc ttcgacgagc
     3181 gattcccgtt tcttttcaac tcctattatg tggccgccgg tccgcgtcat gcgcggccgc
     3241 agcgcggcct gatcacgcgc ccgaacgctg ccgacgtcgc tgcctatcga acgcatgtcg
     3301 acgccgcggt cgagcggctg atcgcgcagg ttccggcagc tgacgctgac cgcgtcttcg
     3361 caattgtgga ggtcgggctg caccacgagc agcagcatca ggaattgctg cttacggata
     3421 ttctccacgc gtttgcgcag aatcccaccg acccggccta cgacgccaaa tggcaggcgc
     3481 cgcgcgcgac gcaaggcccg cgcggttacg tcgacgtgcc cgccggcgtg cacgccatcg
     3541 gtcatgacgg cgagggtttc tgtttcgaca acgagacgcc gtatcacgac g
//
LOCUS       DASSRL010000439         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1254142, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000439 DASSRL010000000
VERSION     DASSRL010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254142"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2132
                     /locus_tag="VFL49_08915"
     CDS             <1..>2132
                     /locus_tag="VFL49_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HET7912140.1"
                     /translation="TFFGSVGLASRTGFDFLTIYIGPALMIGLCSPLLIRIVRLAKAH
                     NITSIADFIAARYGKGQAVAATVALIAIVGTIPYIALQLKAVSSSLETILAHVAAGND
                     ASRPLFGDIALFVALSMATFAVLFGTRHIDATEHQDGLMLAVAAESIVKLVAFLAVGF
                     FVTFWMFDGPIALVNEAMQRPSTATIFSREPRLDTLTAMTLLSFVAFIMLPRQFHVAV
                     VENNNENEIKRAVWLYPVYLVLINLFVVPIALAGLLTFPAGKVDSDMFVLALPLQSGS
                     SLFTLIAFVGGLSAATAMVIVESVALSIMVSNDLIIPFVLQRRERLLSGRENIGSLLL
                     TVRRIAIFSILLLAYVYYRSAGEAQLASIGLLSFAAIAQLAPAFFGGLLWRRATAAGA
                     IAGMTAGFLVWAYTLLLPTLSDIGIIGERILTDGPWGLSVLRPQHLFGLDVPPLVHGV
                     IWSLLFNVLGYIGFSLHRQPSPIERLQANTFVPSDLTPIAPSFRLWRSSVTVEELTTT
                     IARYLGEARTRSAFESFAGAQRISLEPKDDADFRLVRYAEHILASAIGGASSRLVLSL
                     LLRKRTVSIKAALKLLDDANAAIQYNREILQTALDHVRQGIAVFDKELQLICWNRQFG
                     DILDLPPSLVRAGVSLADILRFNGQRGPRAEDHTEEFVSAQMERYVSGNEPFLERFAE
                     GMVIEVRANRMPDGGVVTTFTDITSSVE"
BASE COUNT          341 a          710 c          615 g          466 t
ORIGIN      
        1 ggacgttctt cgggtcggta ggcctcgcct cacgcaccgg cttcgacttc ctgaccatct
       61 atatcggccc cgcgctcatg atcgggttgt gcagcccgct gctgatccgg attgtgcgcc
      121 tggcaaaggc gcacaacatc acctcgatcg ccgacttcat cgccgcgcgc tacggaaagg
      181 gccaagccgt cgccgcgacc gttgcgctga tcgcgatcgt cggcaccatt ccgtacatcg
      241 ccctgcagct gaaggccgtt tcttcctcgc tcgaaacaat tcttgctcat gtcgccgcgg
      301 gcaatgacgc cagccgtccg ttgttcggcg acattgcgct tttcgtcgcg ctgtcgatgg
      361 ccacattcgc cgtgcttttc ggcacccgcc acatcgatgc caccgagcat caggatggat
      421 tgatgctggc ggtcgctgcg gaatcgatcg tgaagctggt ggcgttccta gcggtcggat
      481 ttttcgtgac cttctggatg ttcgacggcc cgatcgcgct cgtcaacgaa gcgatgcagc
      541 gtccttcgac ggccaccatc ttcagccgcg agccgcgcct cgacaccctc actgcaatga
      601 cgttgctgtc ttttgtggcc ttcatcatgc tgccgcggca gttccatgtg gccgtggtcg
      661 agaacaacaa cgagaacgag atcaagcgcg cggtctggct gtacccggtc tatctggtgc
      721 tgatcaatct cttcgtcgtg ccgatcgcgc tggctggact gctgacattc cccgccggga
      781 aggtcgacag cgacatgttc gtgctcgccc tgcccctgca aagcggctcg agccttttta
      841 ctctcatcgc ctttgtcggc gggctgtctg ccgcgaccgc catggtgatc gtcgaatcgg
      901 tcgcgctttc aatcatggtc tccaacgacc tgatcatccc gtttgtgttg cagcggcgcg
      961 aacggctgct cagcgggcgg gaaaacatcg gatctctgct cctcactgtg agacggatcg
     1021 cgatcttctc catcctgctc cttgcctacg tgtactaccg ctcggccggt gaggcccagc
     1081 ttgcttcaat cggcctcctt tccttcgccg ctattgcgca gctggcgccg gcctttttcg
     1141 gcggactgct gtggcgccgc gcgacggccg caggcgcgat cgcgggaatg acggccggtt
     1201 ttctcgtatg ggcctacacc ctgctgctgc ccacgctgtc cgacatcggg attatcggcg
     1261 aacgcattct gaccgacgga ccatgggggc tgagcgtgtt gcggccgcag catctcttcg
     1321 ggctcgacgt gccgccgctg gtgcatggcg tgatctggag ccttctgttc aatgtccttg
     1381 gctatatcgg cttttcgctc caccgccagc cgtcgccgat cgagcggctg caggccaata
     1441 ccttcgttcc ttccgacctg acgccgattg cgccgagctt ccgcctctgg cgctcctcgg
     1501 taacggtcga ggagctcacg acaaccattg cgcgttatct cggcgaagcg cgaacacggt
     1561 ctgccttcga aagttttgcc ggcgcgcaac gcataagcct cgagccgaag gacgatgccg
     1621 atttccgtct cgtccgttac gccgaacaca ttctggcatc cgcgatcggc ggtgcgtctt
     1681 cgcgcctggt cctttcgctg ctgttgcgca aacgcaccgt ctccatcaaa gcggcgctga
     1741 aactgctcga cgacgccaat gcggcaatcc agtacaaccg cgaaatcctg cagaccgctc
     1801 tcgatcacgt gcggcaaggc atcgccgttt tcgacaagga gttgcaatta atctgctgga
     1861 accggcaatt cggcgatatc cttgaccttc cgccgagcct ggttcgggcc ggcgtcagcc
     1921 ttgctgacat cctgcgcttc aacggccagc gcggcccgcg ggccgaggac cacaccgaag
     1981 agttcgtcag cgcacagatg gaacgctatg tctcgggcaa cgagcctttc ctcgagcgct
     2041 tcgccgaagg catggtcatc gaggtgcgcg ctaaccgcat gcccgacggc ggcgtcgtga
     2101 ccaccttcac ggacatcacg tcgagcgtcg ag
//
LOCUS       DASSRL010000440         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_729248, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000440 DASSRL010000000
VERSION     DASSRL010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729248"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFL49_08920"
     CDS             complement(<1..794)
                     /locus_tag="VFL49_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912141.1"
                     /translation="MATNSISLTSGIRSNLLLLQQTSSLLEKTQVRLATGNKINSALD
                     GPAAFFAAKGLTQRAGDLDSLKDGIGQAISTIKAGDSGITGIESLIEQARGLTTQALG
                     SLGNDANSVNLRNSLAQQYNNVLRQIDKLAQDSGYQGKNLLVGSGLRIDATASSKTEV
                     NALTGISGARATNVVDADSYTISVSGDGAISGNSNDIANAERDRGISNLEITGFASKS
                     GFNFDGLSIKLSGGTGKDKTFTVTEGSQTVTQTFTNDQFEAAKTGG"
     gene            1138..>2720
                     /locus_tag="VFL49_08925"
     CDS             1138..>2720
                     /locus_tag="VFL49_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912142.1"
                     /translation="MAVGAVQLPARGQSGQERMTHPAPARDQHKIATLMNQAARAFEA
                     GRIDRLQALLELVLEIDPENAKALYNLAIIYRDRNEIFPAEVHLRRALKVDPDMIDAH
                     QALGDLLFNVKHLLAAAQIYEVALERAPNRLPVLHNLAKTRMMLKDAPETERLARRIL
                     QIDDRSAEGYANLAWSLLYRTMSLDEALAAADRILELVPNSLHGLVLREQALARLGRS
                     AEADDLWQRLLATATGDWATARPLIEVYYWLDLRERSRAITMAFVEANPERTDGFKDL
                     AALLMGEGKFNQAQDVLERAAAMVPGNTTIDMLRGLNAFRLGDYRKGLTLYGARWHRD
                     AFDKPWNVPVPEWDGKPVDGRLLVYCEQGIGDYVMYALLFKELRPLAKSIVIEVNSRI
                     ASLFRRSFPDMRVIDRNALPADWDPSGCAAKVAMGDLPILLNADIENLPNKRGFLVPE
                     PSLAIKLRKRYQALFPGKRLVGISWRSGNRDSATIRSIDLSLWRPILENEACAFVSLQ
                     YGDIKRDIEALQAETGHIV"
BASE COUNT          480 a          856 c          870 g          514 t
ORIGIN      
        1 ctacccccgg tcttcgccgc ctcgaactgg tcgttggtga aggtctgggt gaccgtctgc
       61 gagccctcag tcacggtgaa ggtcttgtcc ttgcccgtgc cgcccgacag cttgatcgag
      121 aggccgtcga agttgaagcc gctcttcgat gcgaagccgg tgatctccag gttgctgatg
      181 ccgcggtcgc gctcggcgtt cgcgatatca ttggagttgc cgctgatagc gccgtcgccc
      241 gacaccgaga tggtgtaaga gtcggcgtcg acaacgttgg tggcgcgggc gccgctgatg
      301 ccggtcaggg cgttgacttc cgtcttgctg ctggccgtcg cgtcgatgcg caggccggaa
      361 ccgaccagga ggttcttgcc ctggtagccc gagtcctgcg cgagcttgtc gatttgccgc
      421 agcacgttgt tgtactgctg ggctaggcta ttgcgcaggt tgaccgagtt ggcgtcgttg
      481 cccagcgaac ccagagcctg ggtcgtgagg ccgcgcgcct gctcgatgag gctctcgatc
      541 ccggtgatgc cggagtcgcc agccttgatc gtgctgatcg cctgaccgat accgtccttc
      601 agactgtcaa gatcgccggc gcgctgggtc agacccttgg cagcgaagaa cgcagcgggg
      661 ccgtcgagcg cggaattgat cttgtttccg gtcgcaaggc ggacctgggt tttttccagg
      721 agcgacgacg tctgctgcaa cagaagcagg ttgctgcgga tgccgctggt caaggaaatg
      781 gagttggttg ccattcgaat gctcctttgg aggcttgctg accgcgacga ctttgcgtgc
      841 gcgggccggc gcagaggcac cgacgggagg gtggggtgca aaatccttgc cacccggcag
      901 aggctgccta gctaacggaa atacttgctt tttcttcggc cagaggtcct ggaaacccgg
      961 caagaattgc cgggtcgggt aaagtttgcc ctgcacttcg gcgcaggatg ggcaaaaatt
     1021 tctctgcgat tccaattgat tagacttcac cattcattaa ccatccgctc cctatcatac
     1081 gtcgtcgcag agccggatca gccgatgcct gcaatttgac gcaaattcga ggcacgcatg
     1141 gcggtcggtg cggtccaact tccggcgcgc ggccaatccg gacaagagcg catgacccac
     1201 cccgcgccgg cgcgcgacca gcacaaaatc gcgaccctca tgaaccaggc ggcgcgggcc
     1261 ttcgaggccg ggcggatcga tcgcctgcag gctctcctcg agctggtact cgagatcgat
     1321 ccggagaacg ccaaggcgct ctacaacctc gccatcatct atcgcgaccg gaacgagatc
     1381 tttcccgcgg aagtccacct gcggcgcgcc ctcaaagtcg accccgacat gatcgatgcg
     1441 caccaagcgc tgggcgacct gctgttcaac gtcaagcatc tgctggcggc cgcccagatc
     1501 tatgaggtcg ccctggagcg cgcgcccaac cggctgccgg tactgcacaa tcttgcgaag
     1561 acccggatga tgctgaagga cgcgccggaa accgagcggc tggcgcgccg gatattgcag
     1621 atcgacgacc gctcggccga aggctatgcc aatctcgctt ggtcgctgct ctaccgcacc
     1681 atgagcctcg atgaggcgct ggccgccgcc gatcgcatcc tcgaactggt gcccaactcg
     1741 ctgcacggac tcgtgctgcg ggagcaggca ttggcgcgtc tcggccggtc ggcggaggcc
     1801 gacgatctct ggcagcggct tctggcgact gcgaccggtg actgggccac cgcccggccg
     1861 ctgatcgaag tctactactg gctggatttg agggagcgca gccgcgccat caccatggcg
     1921 tttgtcgaag ccaatcccga gcgcaccgat ggcttcaaag acctggccgc gctgctgatg
     1981 ggggagggga agttcaacca ggcacaggac gtgctcgaac gcgctgccgc gatggtgccc
     2041 ggcaatacca cgatcgacat gctccggggg ctgaacgcct tccgcctcgg cgactaccgc
     2101 aagggtttga cgctgtacgg cgctcgctgg catcgcgacg ccttcgacaa gccgtggaac
     2161 gtgccggtgc cggaatggga cggcaaaccg gtcgatggcc gcctgctggt ctattgcgag
     2221 cagggcatcg gcgactacgt gatgtatgct ctgttattta aggagctgcg gccgcttgcg
     2281 aaatcgatcg tcatcgaggt gaattcgcgc atcgccagcc tgttccggcg cagcttcccc
     2341 gacatgcggg taatcgatcg caatgccttg ccggcggatt gggatccgtc cggctgcgca
     2401 gccaaggtcg cgatgggcga tttgccgatc ctgctcaacg ccgacatcga gaaccttccg
     2461 aacaagcgcg gcttcctggt accggaaccg tcgctcgcga tcaagctgcg caaacgctac
     2521 caggcgctgt tccccggcaa gcggctggtc ggcatctcct ggcgtagcgg caatcgcgac
     2581 agcgccacca tccggtcgat cgatctcagc ctgtggcgcc cgatcctcga gaacgaggcc
     2641 tgcgccttcg tttccctgca atatggcgac atcaagcggg acatcgaggc cttgcaggcc
     2701 gagacagggc acatcgtgca
//
LOCUS       DASSRL010000441         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1744101, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000441 DASSRL010000000
VERSION     DASSRL010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744101"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFL49_08930"
     CDS             complement(<1..824)
                     /locus_tag="VFL49_08930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7912143.1"
                     /translation="MAHVLDMLRTGAWLTRERVKLVVFGLLAAMLIAVVYVMATSNGL
                     NDRFGRPLGTDFSNVYAAGTYVLDGNAAAPFDPSQQYAREQAIFGKDTPFYGWHYPPY
                     FLGVAALFAAMPYALALALWQGVTFTLYLWVTRGILKSSSWPGLSRPSTSFLSTMPQD
                     VDARHIGVRRHAVLRTAIAGHDEALPCPTNDPLWLPLVLGFPAVFVNLGHGHNGFLTA
                     ALFGAALLQLDRRPVLAGILFGCIAYKPQFGLLIPIVLAASGRWRVFTATAATVAI"
     gene            974..2179
                     /locus_tag="VFL49_08935"
     CDS             974..2179
                     /locus_tag="VFL49_08935"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7912144.1"
                     /translation="MAFLAEQLKRIKPSATIAVTDKARALKLAGRNVIGLGAGEPDFD
                     TPDNIKQAAIEAIKSGKASKYTNVDGIAELKDAIVRKFKRENGLDYKPTQVTVGTGGK
                     QVLYNAFVATLNPGDEVIIAAPYWVSYPEMVLLAGGEPVEVVTSMANGFKMSAQQLER
                     AITPKTKWVLLNSPSNPSGAAYTRAELKALTDVLVKHPHVHVMTDDMYEHLVYDDFAF
                     TTPAQVEPSLYPRTLTINGCSKAYCMTGWRIGYAGGPEPLIKAMATVQSQSTSNPTAV
                     SQWAAVEALTGPQDFIAKHNKIFKERRDLVVSMLNQANGIKCPTPEGAFYVYPSCAGT
                     MGKTAPTGKKLASDEDFVTELLEAEGVAVVQGSAFGLGPAFRISYATKTEDLEEACRR
                     IQRFCGNLK"
     gene            2378..3382
                     /locus_tag="VFL49_08940"
     CDS             2378..3382
                     /locus_tag="VFL49_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912145.1"
                     /translation="MIRRTVAIFIVISNLSFAANAQERVVIGTQRLTENGALFLAAAQ
                     GYFKAEGIDLAMTAYDSDKAVAETLASGATDFGLARFTPAAFDAAGRGLIKAVAAQAR
                     EKRDYEGSQLVASSIGLQKGLHKFDNLAGKTIAIDALGSTAHYQLQQIARIKHVDPAR
                     ITIKPLGTLDAIARALGTDQVDAAIMPALYARELLVANQAKFLGWYSELDEQQLGALF
                     VSKKMIDTRRVIVEKFVHAYRRGAADYAAALLRKDRSAKRVSDIKSKEAATTIARYVY
                     PDRGTDAAAAVELGAYYIDPQARLDVADIARQIAWCKSQGLIDKAVDAHAVVDSSFVK
                     "
     gene            3497..>3988
                     /locus_tag="VFL49_08945"
     CDS             3497..>3988
                     /locus_tag="VFL49_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7912146.1"
                     /translation="MGQNGSGPNGTRSSGPRCIALVGPFQSGKTTLLEAILARTGAIQ
                     RQGTVDAGTTVGDASKEARHHKMSVEATFATTTFMGDNYTFVDCPGSIEFIQDMRSVL
                     PAVDAAVVVCEADEKKVPQLQLILRELEDQNIPHFLFLNKIDKADKRVRETIKLLQPA
                     SRAP"
BASE COUNT          850 a         1217 c         1239 g          682 t
ORIGIN      
        1 aagatcgcaa ccgttgcagc ggtagcggtg aagacgcgcc accgcccgct tgccgccagc
       61 acaatcggaa tgagcaaccc gaattgcggt ttgtacgcga tgcagccgaa cagaattccg
      121 gcgagaaccg gccgacggtc gagctgcagc aatgccgccc cgaacagggc agcggtgagg
      181 aagccgttgt ggccgtgccc gagattgacg aagacagccg ggaagccgag gacgagcgga
      241 agccagagcg gatcgtttgt agggcacggc aaagcttcgt catgcccggc aatagccgtt
      301 cgaagaacgg cgtgccttcg cacgcctatg tgccgggcat cgacgtcttg cggcatggtc
      361 gagagaaaag acgtggatgg ccgggacaag cccggccatg acgaactctt tagaatcccc
      421 cgcgttaccc agagataaag agtgaacgtg acgccctgcc aaagcgcgag cgccagcgca
      481 tagggcatcg cggcgaacag tgcggcgact ccgaggaagt agggcggata atgccagccg
      541 taaaagggtg tatccttgcc gaaaatcgcc tgctcgcgcg cgtattgctg cgacgggtca
      601 aagggggcgg cagcgttgcc gtcaaggaca taagttccgg cggcatagac gttggagaaa
      661 tccgtgccga gcggacggcc gaagcggtcg ttgaggccgt tcgaggtagc cattacgtac
      721 acgacggcga tcagcatcgc ggcgagcagc ccgaagacga cgagctttac ccgctcgcgc
      781 gtcaaccagg cgccggtgcg cagcatgtcc aggacatgtg ccatgcccaa agggactagc
      841 aactgtgtct taagggacgg ttaggccatc aacagccttg atcatgacca tcagcctttg
      901 cgacttgccg tcgcctcaat ggcccattac gacatgcgca ggggcatttt ttcgccccgg
      961 attcggagtt tccatggcct ttctcgcgga gcagctcaag cgcatcaagc cgtcggcgac
     1021 catcgccgtc acggacaagg cgcgcgcgct caaactggct ggccgcaacg tcatcggcct
     1081 gggcgcgggc gagcccgact tcgacacgcc cgacaacatc aagcaggccg cgatcgaggc
     1141 gatcaaaagc ggaaaggctt cgaaatacac gaatgtcgac ggcatcgccg agctcaagga
     1201 cgccatcgtc aggaagttca agcgcgagaa cggccttgac tacaaaccga cccaggtcac
     1261 ggtcggcacc ggcggcaagc aggtgctgta caacgcgttc gtcgcgacgc tgaacccggg
     1321 cgatgaggtg atcatcgcag cgccctactg ggtcagttac ccggaaatgg tgctgctcgc
     1381 cggtggtgag cccgtagaag tcgtcaccag catggcgaac ggcttcaaga tgagcgcgca
     1441 gcagcttgaa cgcgcgatca cgccgaagac caagtgggta ttgctgaact cgccgtcgaa
     1501 cccgagcggc gctgcgtaca cgcgcgccga gctcaaggcc ctcacagacg tgctggtgaa
     1561 gcatccgcac gtccatgtga tgaccgacga tatgtatgag catctcgtct acgacgactt
     1621 cgcgttcacg acgccggcgc aggtggagcc ctcgctttat ccgcgcacgc tgacgatcaa
     1681 cggctgctca aaggcctatt gcatgaccgg ctggcgcatc ggctatgccg gcgggcccga
     1741 gcctttgatc aaggcgatgg cgacggtgca gtcacagtcg acgtcgaacc cgaccgccgt
     1801 atcgcaatgg gcggcggtcg aagcgctgac tgggccgcaa gatttcatcg ccaagcacaa
     1861 caagattttc aaggagcggc gcgatctcgt cgtgtcgatg cttaaccagg cgaacggcat
     1921 caagtgtccg acgccggaag gcgccttcta tgtctatccg tcctgcgccg gcacgatggg
     1981 caagacggcg ccgacgggca agaagctggc gagcgacgaa gatttcgtca ccgaactgct
     2041 cgaggctgaa ggcgtggccg tcgtgcaggg ctcggccttc gggcttgggc ccgcattccg
     2101 catttcctat gcgaccaaga ctgaagacct ggaagaggcc tgccgacgca tccagcgttt
     2161 ctgcggcaat ttgaaatagc aaccacatcc actcgtctcc gcgcacgcgg ggatccggca
     2221 atcattatca agcagatcga gaactctgga ttcccgcata ggcgtacgga agcacgccgt
     2281 ccttcggacg gctatgcgcg cgaatgagcg gaataatgcg cgaatgcagc tattcttgat
     2341 tcaccttctc ttatgcgggc tccgctaatc tgcgggcatg attcggcgca cggttgcgat
     2401 ttttatcgtc atttccaatc tgtccttcgc cgcaaatgcg caggagcgtg tcgtcatcgg
     2461 gacgcagcgg cttactgaaa acggcgcgtt gttcctggcc gcggcgcaag gctacttcaa
     2521 ggccgaaggc atcgacctgg cgatgaccgc ctacgattcc gacaaggctg ttgcggaaac
     2581 gctcgcttcc ggcgcgacgg acttcggcct cgcgcgcttc acgccggcgg cgttcgatgc
     2641 ggccggcagg ggcctgatca aggcggtcgc ggcgcaggcg cgcgagaaac gcgactacga
     2701 aggcagtcag cttgtcgctt cgagcatcgg gctgcaaaaa ggcctgcaca agttcgacaa
     2761 cctggcgggc aagacgatcg ctatcgatgc gctcggatca accgcgcatt accagctgca
     2821 gcagatcgcg cgcatcaagc acgtcgatcc ggctcgcatc accatcaagc cgcttgggac
     2881 gctcgacgcc atcgcgcgcg ccctcggtac cgatcaggtc gatgcggcga tcatgccggc
     2941 gctctacgca cgcgagctgc tggtcgcgaa ccaggccaag ttcctcggtt ggtattcgga
     3001 gctcgacgag caacagctgg gtgcgttgtt cgtttcgaaa aaaatgatcg atacgcggcg
     3061 tgtcatcgtc gaaaaattcg tgcacgccta tcggcgcggc gccgccgact atgctgctgc
     3121 cttgctccgc aaggatcgca gcgcgaaaag ggtttccgac atcaaatcga aggaggcggc
     3181 aacgacgatc gcgcgttacg tctatcccga ccgtgggacc gatgcagccg ccgcagtcga
     3241 gctcggcgcc tattacatcg atccgcaggc gcgactcgat gtcgccgaca tcgcgcgcca
     3301 gatcgcctgg tgcaagtcgc aggggctgat cgacaaggct gtcgatgcgc acgcggtcgt
     3361 cgattcaagt ttcgtcaagt agcaaatctt ctgcacggcg aatctctgcc cttgccagtc
     3421 tccgtgcgtc ctgagacgat gacgtcacag ggccgggaaa ccaggtccga cctcccggcc
     3481 ggcgagggag gcttttatgg gacaaaatgg ctctggtccc aatggcacaa ggagttcggg
     3541 tccacggtgc attgcactgg tgggcccatt tcaaagcggc aaaaccacat tgctggaagc
     3601 gatcctggcc cgcaccggtg cgatccagcg ccagggcacc gtcgatgcgg gtaccacggt
     3661 aggtgacgcg agcaaagagg cccgccacca caagatgagc gtcgaggcga ccttcgccac
     3721 gaccacgttc atgggcgaca actatacgtt cgtcgattgc ccgggctcca tcgaattcat
     3781 ccaggacatg cgcagcgtgc tgccggcagt cgatgccgcg gtcgtcgtct gcgaggcgga
     3841 cgagaagaaa gtcccgcagc ttcagctgat cctgcgcgag ctggaagacc agaacattcc
     3901 gcacttcctc tttctcaaca aaatcgacaa ggccgacaag cgggtccgcg agacaatcaa
     3961 attgctgcaa ccggcatcac gtgcaccg
//
LOCUS       DASSRL010000442         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_606480, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000442 DASSRL010000000
VERSION     DASSRL010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606480"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1410)
                     /gene="rho"
                     /locus_tag="VFL49_08950"
     CDS             complement(145..1410)
                     /gene="rho"
                     /locus_tag="VFL49_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET7912147.1"
                     /translation="MREMKLQDLKSKTPAELVAFAEEHQVENASTMRKQELMFAILKQ
                     LATQEVDIIGEGVVEVLSDGFGFLRSPESNYLSGPDDIYVSPSQIRRFGLRTGDTVEG
                     HIRSPKEGERYFALLKVNTINFEDPEKARHKVNFDNLTPLYPDERLRMEYEDPTKKDL
                     SARVIDIVAPIGKGQRALIVSPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHQRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVI
                     FEEFKGTGNSELILDRKVADKRTFPAIDITRSGTRKEELLTDPAILKKMYVLRRILNP
                     MGTMDAIDFLLDKLRNTKSNADFFESMNT"
     gene            complement(1674..2096)
                     /gene="hemJ"
                     /locus_tag="VFL49_08955"
     CDS             complement(1674..2096)
                     /gene="hemJ"
                     /locus_tag="VFL49_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HET7912148.1"
                     /translation="MYLWLKALHIVAVIAWMAGMLYLPRLFVYHAVSDVGSPQSETFK
                     TMEHRLLNFIMVPAMAITWIAGIALVLLGQWFSAGWFQAKFIIVVAMTVMHGLFAHWV
                     NEFRHDRNSHSQKFFRIINEIPTVLLVLIVILATVKPF"
     gene            complement(2103..>3106)
                     /gene="hemE"
                     /locus_tag="VFL49_08960"
     CDS             complement(2103..>3106)
                     /gene="hemE"
                     /locus_tag="VFL49_08960"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7912149.1"
                     /translation="VLKGKREVIPPIWLMRQAGRYLPEYRAIREKAGGFLDLCFNPNL
                     AAEVTLQPIRRFGLDAAILFSDILVIALALGRKVEFLAGEGPKLEPLADASALPAMRK
                     EADQTVLAPIYETVRQAKAMLGDTTALIGFCGAPWTVATYMVAGQATPDQAPARLFAY
                     RDPENFSRLIDRLVDASIEYLTGQLEAGADCVQIFDTWAGVLGPEQFDRWCVAPTQKI
                     VEGVRRRIPFAKIIGFPRGAGAMALPYVEMTGVSAIGIDWTFERTIARDALQSRVPVQ
                     GNVDPLALLAGGAALDREVDDVMRHLGDGPLIFNLGHGILPDTPVAHVDRMLKRVRR"
BASE COUNT          660 a          885 c          919 g          642 t
ORIGIN      
        1 tcccgccatc acacccggcg caggaaaagc tgaaggccgc accgggaagc gcggccttcg
       61 tatgcgtcgg tgagcgctgc tcgcttccgg taaccgatgc cgctcagatt ggcccggctg
      121 ttttggccat gcgtcaataa ccggtcacgt attcatcgat tcgaagaagt cggcgttgct
      181 ctttgtattg cgcagcttgt cgagcaggaa gtcgatagcg tccatcgtgc ccatcgggtt
      241 cagaatcctg cgcaaaacgt acattttctt gaggattgca ggatcggtga gaagctcttc
      301 cttgcgtgtg ccggaccgcg tgatgtcgat cgcaggaaac gtgcgcttgt cggcgacctt
      361 gcggtcgagg atcagttccg agttaccggt gcccttgaac tcttcgaaaa tcacttcgtc
      421 catgcggctg ccggtatcga tcagagccgt cgcgatgatg gtgagtgatc cgccttcctc
      481 gatgttgcgg gccgcgccga aaaagcgctt tggccgctgc agcgcgttgg cgtcgacgcc
      541 accagtcagc accttgccgg aagagggcac gacggtgttg taggcgcggc cgagacgcgt
      601 gatcgaatcg agcaggatca cgacgtcgcg ctgatgctcg accaggcgtt tcgccttttc
      661 gatcaccatt tcggcgacct gcacgtggcg cgaggcaggt tcgtcgaacg ttgatgagat
      721 cacctcgccc ttcaccgagc gctgcatgtc ggtgacttct tccgggcgct cgtcgatcag
      781 gagaacgatc agataacact cgggatgatt ggcggtgatc gaatgcgcga tgttctgcag
      841 cagcacggtc ttgccggtgc gcggcggcga gacgatcagc gcacgctggc ctttgccgat
      901 cggcgcgaca atatcgatga cccgcgcgga gaggtccttt ttggtcggat cttcgtactc
      961 catccgaagc cgctcgtcgg gataaagcgg ggtgaggttg tcgaaattga ctttatgccg
     1021 cgccttctcg ggatcctcga aattgatggt gttgaccttg agcagcgcga aatagcgttc
     1081 gccttctttc ggtgagcgga tgtgaccttc gactgtgtca ccggttcgca gaccgaaacg
     1141 gcggatctgg ctcggcgaaa catagatgtc gtccggcccg gacagataat tcgattccgg
     1201 tgagcgcagg aatccgaagc cgtcggagag cacttcgacc acgccttcgc cgatgatgtc
     1261 gacttcctgc gttgcgagct gcttcaggat cgcgaacatc agctcctgct tgcgcatggt
     1321 gctggcattc tcgacttggt gttcttcggc aaacgcgacg agttcggcag gagtcttgga
     1381 cttgaggtcc tgaagtttca tttcccgcat tgggggttta cgtccttgga agcgttaccg
     1441 gcgaaggttt gaaaacaaaa aacttgcgcc gtttgacttt ggagagagat cgcaggtctg
     1501 gggtgacgag gttgtagtag ggaatggccc ggaacccgat gctgcgaccg gcccaaacac
     1561 gagggagccg gaacgcgact gcatccgcct gcgttcggtg ggatggcgcc aagataagtt
     1621 gagcgaccgt ccggcgcaag agggcagacg cgtgtgagca actgcgggga tgtctagaac
     1681 ggcttaacgg tcgccaggat gacaatgagg accaacagga ccgtaggtat ctcattgata
     1741 atacgaaaga acttttgact gtgtgaattc cggtcgtggc ggaattcgtt tacccagtga
     1801 gcaaaaaggc cgtgcatgac ggtcatcgcg acgacgatga tgaatttggc ctgaaaccac
     1861 ccggcgctga accattgccc gagcagcacc aaagcgatgc ccgcaatcca ggtgatcgcc
     1921 atggccggca ccatgatgaa gttgagaagc cggtgctcca tcgtcttgaa ggtttccgat
     1981 tgcggggagc caacatcgga aaccgcatgg tagacgaaga gccgcggcag atagagcata
     2041 cccgccatcc aggcgatcac ggcaacgatg tgcaacgctt tcagccagag atacatcgtg
     2101 gcctacctgc gcacacgttt aagcatgcgg tcgacatgcg caactggcgt gtccggcaat
     2161 atcccatgtc cgaggttgaa gattagcggg ccgtcgccga ggtgacgcat cacatcgtcg
     2221 acctcgcgat cgagagctgc accgccggcc agaagcgcaa gcggatcgac gttgccttgc
     2281 accgggacac gtgattgcaa cgcatcgcgc gcaatagtgc gctcaaaggt ccaatcaatg
     2341 ccgatcgcgc tcacgccggt catttccaca tagggcagag ccattgcgcc ggcgccacgt
     2401 ggaaatccaa tgatctttgc gaaaggtatt cgccgacgta cgccctcgac aatcttttgc
     2461 gttggcgcga cacaccagcg gtcgaactgt tcaggaccaa gcacaccagc ccaggtgtcg
     2521 aaaatctgta cgcagtccgc acctgcctcg agctgcccag tcaggtattc gatcgatgca
     2581 tccaccagac gatcgatcag ccgcgaaaaa ttctcaggat cgcgataggc aaacagacgt
     2641 gccggcgcct gatcgggcgt tgcttgccct gcgaccatgt acgttgcaac agtccatgga
     2701 gctccgcaaa agcctatcag cgccgtcgta tcaccaagca tggctttggc ctggcgcacc
     2761 gtttcataaa tcggtgctag aacagtttga tcggcttcct tccgcatagc aggaagcgca
     2821 ctcgcatccg ccaaaggctc aagcttcggc ccttcaccgg ccagaaattc aactttccgg
     2881 ccgagtgcca gcgcgatcac aaggatatcc gagaatagaa tcgctgcgtc caagccaaac
     2941 cgccggattg gttgcaacgt cacttctgca gccagattcg gattgaaaca gagatccaga
     3001 aaccctcccg ccttttcgcg gattgcacga tattccggaa ggtaccggcc agcctgtcgc
     3061 atcagccata tcggcgggat cacctcacgt ttgcctttca ggacgc
//
LOCUS       DASSRL010000443         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_624280, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000443 DASSRL010000000
VERSION     DASSRL010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624280"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFL49_08965"
     CDS             complement(<1..396)
                     /locus_tag="VFL49_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7912150.1"
                     /translation="MTRWAMVADLERCVGCQTCTAACRHANATSPAVQWRKVLDIEAG
                     TYPNVSRIFVPVGCQHCADPPCMHVCPTTATRQRADGIVTIDYDLCIGCAYCEVACPY
                     QARFKIDAPSFAFGDGATQDEVRREEPRRL"
     gene            complement(393..3131)
                     /locus_tag="VFL49_08970"
     CDS             complement(393..3131)
                     /locus_tag="VFL49_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7912151.1"
                     /translation="MPGKSAHNERTVSTYCYQCVAGPDLLKVRVEDGIATEVTPNFDA
                     AVVHPAGGKACVKAFGLVQKAYNPNRIFYPMKRTNPVKGRDQDPRFVRISWEEALALV
                     ADKLNAVRASGLSDDSGYPRVAASFGGGGTPTAYMGTLPAFLSAWGPIDMSFGSGQGV
                     KCYHSEHLYGELWHRGFTVSPDTPLCEYLVSFGANVEASGGVCGVKRHADARKRGMKR
                     VQIEPHLSVTGACSAEWIPIRPKTDSAFLFAMIHVFLHEHARERLDIPFLKHRTSSPY
                     LIGPNGLFMRDAKTRKPLMMDAVTGRAVPFDAAGVDPALEGSFVADGIEVHADDTVIS
                     HQKVAVSPAFAHLVTHVGSYSPEWAATICDVPADTIRRVANEFLEHARVGETIDIEGV
                     TLPLRPVSISLGKTVNNGWGGYECCWARTLIGCLFGALEVPGSTLGTTVRLNRPADNR
                     WSSVKPGPDGFMQYPMNPTSKADWISRPTARSAHRMLVPLVADSAWSQALGPTHLAWM
                     MQDENFDQLPEASSPDVWFVYRTNPAISFWDTKAVGKAMAKFPFVVCFAYTRDETNHV
                     ADVLLPDCTDLEGLQLLRIGGTKYVEQFWDHQGFALRAPAVPPQGEAKDFTWIATELA
                     RRTGLLEAYNSAINRGAAGIRLSGKNYDFSLAPEKVHDVETIWDASCRAASAEITDGA
                     ESQGLAWFREHGFRVKPFPRLQWYLYPKLVAEGLRFEQPYQEQLFRIGKELGRRLHER
                     NIHWWDKQLSEYEALPHWHDLNALWESALAKHYSVRIADYPFWLITARSMQYSWGGNV
                     GIQLIKEVADNVAGHGNVVMNHGAAVRLGIADGDVVEVQSPLNKTKGRVMLCEGIRPD
                     TLLMIGQFDHWATPFAKDFHAPSMNALMPMLLDLTDATGSSADLVKVTIRRSESVA"
     gene            complement(3137..3547)
                     /locus_tag="VFL49_08975"
     CDS             complement(3137..3547)
                     /locus_tag="VFL49_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7912152.1"
                     /translation="MKESLRPGLSRTSRIEIDRDRTIGFMGEEGRVYGTPYLVRDIEV
                     TCRQLILDHADAGEDSVGTDVAIKHLAPTLLGMSVEITVTVAAVEGRKIVFDISAKDN
                     IEQISAGTHGRFVVDVDKTIQRLKAKAGKLAASG"
     gene            complement(3638..>3842)
                     /locus_tag="VFL49_08980"
     CDS             complement(3638..>3842)
                     /locus_tag="VFL49_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912153.1"
                     /translation="NLVDQVLSAIRALKNDKLTILLVEQNARAALSIADRAYVMETGK
                     ITLSGSAAEIQSDRRVREAYLGI"
BASE COUNT          807 a         1139 c         1072 g          824 t
ORIGIN      
        1 gaggcggcgc ggctcctcgc gacggacctc gtcctgcgtc gcgccgtcgc cgaatgcgaa
       61 gctcggcgcg tcgatcttga accgcgcctg gtacggacag gcgacctcgc aataggcgca
      121 gccgatgcag agatcgtagt cgatcgtaac gatgccatct gcgcgctgac gcgtagctgt
      181 tgtcggacac acatgcatgc aaggaggatc ggcgcagtgc tgacaaccga ccggtacgaa
      241 gatgcggctg acgttcgggt aagtgccggc ttcgatatcg agcaccttac gccactgcac
      301 tgccggtgat gttgcattgg catgccggca cgctgccgtg catgtctgac agccgacgca
      361 ccgttcaaga tcggctacca tcgcccagcg tgtcatgcca cgctctccga gcgtcttatc
      421 gtgaccttga cgagatcagc gctcgatccg gtagcatcgg tgagatcaag cagcataggc
      481 attaaggcat tcatgcttgg agcatggaaa tccttggcaa agggcgttgc ccaatgatca
      541 aactggccaa tcatcagcag cgtgtcggga cggatgccct cgcacagcat gactcgtcct
      601 ttggttttat tgagcggcga ttgcacttct acgacgtcgc catcggcaat gccgagacgt
      661 accgcggcgc cgtggttcat gacgacattg ccgtggccag cgacgttgtc cgctacttct
      721 tttatgagct ggataccgac attgccgccc caggagtact gcatgctgcg ggcggtgatc
      781 agccaaaacg ggtaatcggc gatgcgtacg gaatagtgct tcgccagcgc gctttcccat
      841 aaggcgttga ggtcatgcca gtggggtagc gcttcatatt cgctgagttg cttgtcccac
      901 caatgaatgt ttcgttcatg caaccggcga ccaagctcct ttccaatgcg aaatagctgc
      961 tcctggtaag gctgttcgaa gcgcagtcct tccgcgacaa gcttcggata taagtaccat
     1021 tgcagccgtg gaaacggctt cacgcggaat ccgtgctcgc ggaaccacgc gagtccctgg
     1081 gattccgcgc catcggtgat ttccgcgctg gcggcgcggc aactcgcgtc ccagatcgtc
     1141 tcgacatcgt gaactttttc cggtgcgagc gaaaagtcgt agttttttcc ggaaagtctg
     1201 atgcccgcgg caccgcggtt gatggccgaa ttgtaagcct cgagcagccc ggtgcggcgg
     1261 gcgagctcgg tcgctatcca cgtgaagtct ttggcctctc cctgcggtgg caccgccggc
     1321 gcgcgaagcg caaatccctg gtgatcccag aattgctcaa cgtatttggt cccgccgatg
     1381 cgcagcaatt gcaggccttc aagatcggta cagtcaggca gaaggacatc cgcaacgtga
     1441 ttggtctcat cgcgcgtgta agcaaagcag accacaaacg gaaatttcgc catcgccttg
     1501 ccgacagctt tggtatccca aaaggaaatc gccggatttg tccgatagac aaaccagaca
     1561 tccggcgatg aagcctccgg aagctggtcg aaattttcgt cttgcatcat ccaggccaga
     1621 tgcgtcggac cgagggcctg actccatgct gagtcggcga cgagtggcac caacatccgg
     1681 tgtgcgctgc gtgcagttgg acgcgatatc caatcggctt tgctggtcgg attcatcgga
     1741 tactgcatga agccatcagg acccggcttg acgctcgacc accgattgtc ggccggccga
     1801 ttgagcctga ccgtcgtgcc aagagtgctg cctgggactt ccaacgctcc aaacaaacat
     1861 ccgatcagcg tacgcgccca gcagcattcg tatccgcccc agccgttgtt gacagtcttt
     1921 cccagcgaaa tcgataccgg ccgtaaaggc agtgtcacgc cttcaatatc gatcgtttcg
     1981 cctacgcgcg cgtgttcgag aaattcgttg gcgacgcggc ggatggtatc cgccggcaca
     2041 tcgcaaattg tcgccgccca ttctggcgaa taactcccta catgagtgac gaggtgggcg
     2101 aatgcaggac tgaccgccac tttctgatgc gaaatgaccg tatcatctgc gtgtacctcg
     2161 attccgtcgg cgacaaatga gccttccaat gccggatcga caccggcggc atcaaacggt
     2221 accgcgcggc cggtcacggc atccatcatg agcggtttgc gcgttttggc gtcgcgcatg
     2281 aacagaccat tcggtccgat caggtaaggc gaggacgtcc gatgcttgag aaacgggata
     2341 tccagccgct cacgcgcatg ctcgtggagg aaaacatgaa tcatcgcaaa cagaaatgcc
     2401 gaatccgttt ttggcctgat tggaatccat tcggctgagc acgcaccagt gaccgaaaga
     2461 tgcggttcta tttggacgcg tttcatgcct cgcttgcgag catccgcgtg ccgctttacg
     2521 ccacatacac cgccggatgc ttcgacattc gcgccgaagg aaacaagata ctcgcaaagc
     2581 ggcgtgtctg gtgagaccgt aaagccccgg tgccacagtt cgccataaag gtgctcggag
     2641 tgatagcact tcacgccctg gccggagcca aagctcatgt cgatcgggcc ccacgccgac
     2701 aagaacgccg gcagcgttcc catgtaggcg gttggcgtgc caccgccgcc gaagctcgcg
     2761 gcgacgcgcg gatatcctga atcgtcgctc aacccgctcg cccgcacggc attgagcttg
     2821 tcggcaacga gcgctaacgc ttcctcccag gaaatgcgca caaagcgcgg atcctggtcg
     2881 cggcccttga ccgggttcgt gcgcttcatc ggatagaaaa tccgatttgg attgtaggcc
     2941 ttctgcacaa ggccgaacgc cttcacacac gctttgccgc cggctggatg gacaaccgct
     3001 gcgtcgaaat tcggggtgac ctcggttgca atcccgtctt caacacgaac tttcaataga
     3061 tccggcccag caacacactg gtagcaatag gtcgagaccg ttcgttcgtt gtgcgcggac
     3121 ttacccggca tgatgtttat cccgaagcgg caagctttcc ggcttttgcc tttaagcgtt
     3181 gaatggtctt gtcaacgtct acgacgaaac ggccatgcgt tccggcactg atctgttcga
     3241 tgttgtcttt ggccgatata tcgaacacga tcttgcgacc ttcgactgca gccacggtaa
     3301 ctgtgatctc aaccgacatg ccgagcagcg tcggagcaag atgcttgatc gcgacgtcag
     3361 tgccgaccga gtcctctccc gcatcggcgt gatcgagaat caattgccgg caggtcactt
     3421 ctatatcgcg caccagatag ggcgtaccgt aaacgcgccc ctcctcaccc atgaatccga
     3481 tagtgcggtc gcgatcaatt tcgatgcggc tggtccgcga taatcccgga cgaaggcttt
     3541 ccttcaccag tttctcctac ccgtaagaat tcaaccaagg tgcaactata atcattttca
     3601 cctgctcaac ttgactaagg tcaattcttc cagtgtttta gatacctaaa taggcctcgc
     3661 gaacgcgccg gtcagactgg atttcggctg cggatccact cagagtgatt tttccggttt
     3721 ccatgacata ggcgcggtcc gcgatcgaaa gcgccgcgcg cgcattctgt tccacaagca
     3781 agatcgttag tttgtcattt ttgagcgcgc gaattgcaga cagcacttga tcaacgagat
     3841 tt
//
LOCUS       DASSRL010000444         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1358952, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000444 DASSRL010000000
VERSION     DASSRL010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358952"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFL49_08985"
     CDS             <1..370
                     /locus_tag="VFL49_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912154.1"
                     /translation="AMTANELGLWRATDGKLTALANVGPANPREFAEVTSTPDVLQAL
                     SSATGGDARRLAENNTLNVPRVVPVRTSTTFKGDDWIGLRMRDVSVVRGIGVLPIFAG
                     LIGLLLLVGSLAVTWAREGR"
     gene            complement(367..876)
                     /locus_tag="VFL49_08990"
     CDS             complement(367..876)
                     /locus_tag="VFL49_08990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7912155.1"
                     /translation="MSEPALTILPETVADAPAIERLHERTFGPGRFARTAFRIREGRG
                     HALDLSFTARIGTLMVGSVRLTPICIGETPALLLGPLTVEPPFRSHGVGTALVKRALH
                     EAKSSGHKLVVLVGDEPIYAKSGFKRIPKGQVKMPGPVDPARLLVAELATGAFDGVAG
                     LIRPDWETA"
     gene            998..1975
                     /locus_tag="VFL49_08995"
     CDS             998..1975
                     /locus_tag="VFL49_08995"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7912156.1"
                     /translation="MGLLVDGVWQEDVSRTKDGHFIRPTTKFRNWVTPDGGPGPSGKG
                     DFAAEAGRYHLYVSLSCPWAHRTIIFRKLKALENVVSMSIVSPDMLKNGWTFNVSEGS
                     TGDSANGKSRLSEIYLLAEPRYTGRVSVPVLWDKKRKTIVNNESSEIIRTLNSAFDAF
                     TNVRTDYYPEPLRAQIDKFNDLIYPNINNGVYRAGFATSQAAYEEAFRNVFDTLDELD
                     QVLSQHRYLVGNTITEADWRLFCTLIRFDAVYYSHFKCNWRHIYEYPNLSNYLRDLYQ
                     VPGVAETVSIEQIKRHYYGSQRQVNPTGIIPVGPQLDFAAPHDRARFSA"
     gene            complement(1979..2464)
                     /locus_tag="VFL49_09000"
     CDS             complement(1979..2464)
                     /locus_tag="VFL49_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7912157.1"
                     /translation="MEHLHRLRRLLEPVISRAMHFYWRFSRPATLGARAMVIDGAGRV
                     FLVKHSYVDGWHLPGGGVETGETFVDALARELVEEGNIRLGASPQLFGIYLNKRVSRR
                     DHVALFIVRDFRQDGEPRPNHEIVAHGFFHPDALPEDTGRGTRARLAEVFGRAPVSQV
                     W"
     gene            2592..3551
                     /locus_tag="VFL49_09005"
     CDS             2592..3551
                     /locus_tag="VFL49_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET7912158.1"
                     /translation="MKRFADLSEQEVLALAITNEEEDSRIYRGFAEGLREQFPASAKV
                     FDEMADEEVHHRTMLFDLYRKKFGDYLPLIRRQDVKGFLHPKQPLWLTRPLGLEEVRK
                     FAENMEFEAERYYRRAADNARDASIRQLLVELADAEAGHESLAHKLSEEILTKGARAK
                     EDETARRMFVLQYVQPGLAGLMDGSVSTLAPLFAAAFATHNTWETFLVGMAASVGAGI
                     SMGFAEALSDDGSLTGRGAPLLRGTVCGVMTAIGGLGHTLPFLIPDFYVATVVAVLVV
                     VVELAVISWIRTRFMDTPFLQAAFQVVVGGVLVFIAGILIGSS"
     gene            3562..>4172
                     /locus_tag="VFL49_09010"
     CDS             3562..>4172
                     /locus_tag="VFL49_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7912159.1"
                     /translation="MLTLAHLSDPHLAPLPQPRWSELAGKRMTGYINWQRKRRFVHDA
                     AVLSAIAADVRAQTPDHIAVTGDIANIALPAEFLRGREWLESLGSSRDVTFVPGNHDI
                     YVREAGALADRQLSAYMCDDDGESGFPFVRRCGDIALIGLSTGVPTAPFLATGWLGTR
                     QLAEFAAILNNLKDENFFRIILIHHPPMTNAARHKRLMDASIL"
BASE COUNT          805 a         1290 c         1282 g          795 t
ORIGIN      
        1 ggcgatgact gccaacgagc tggggctctg gcgcgcaacc gatggcaagc ttactgcgct
       61 tgccaatgtc gggcctgcca acccgcgtga gttcgccgaa gtaacctcga cgcccgatgt
      121 gctgcaggcg ttgagcagcg cgaccggcgg cgatgcgcgc cgcctggcgg agaacaacac
      181 tctcaacgtg ccccgcgtgg tgccggtacg caccagcacg acgttcaagg gtgatgactg
      241 gatcggcctc aggatgcgcg acgtcagtgt cgtgcgcgga atcggcgtgc ttcctatttt
      301 cgccggtctg atcggcctgc tcttgctggt cggttcgctg gcggtgacct gggcgagaga
      361 aggccgctag gccgtttccc agtccggccg gatcaagcct gccacgccat cgaaggcgcc
      421 ggtcgcgagt tccgccacca gcaggcgcgc aggatcaacc ggacccggca tctttacttg
      481 ccccttcgga atgcgcttga acccggactt ggcgtagatc ggctcgtcgc cgaccagcac
      541 caccaatttg tggccactgc ttttcgcctc atggagtgcg cgcttgacca aagccgtgcc
      601 gacgccgtgc gagcggaacg gcggctcgac tgtgagcggg cccagcagca acgccggcgt
      661 ttcgccgatg cagatcggcg tcagccgaac agagccgacc atcagcgtgc cgatgcgtgc
      721 cgtgaatgac agatcgagcg cgtgccccct gccctcgcga atgcggaaag ccgtgcgcgc
      781 gaaacggccc ggcccgaagg tgcgctcatg cagacgctcg atcgcagggg cgtcggcgac
      841 ggtctcaggc aggatggtga gcgccggttc gctcatgggg ttaaatatcc gtgtcgggcg
      901 ctgccgctta gcatttgggc cgattctggt ccattgcccg tggcgcaacg ggtcgcaaca
      961 gcctaaatga acagcggtga aggttaagga gatcggcatg ggacttctgg tcgacggcgt
     1021 ctggcaggaa gatgtttcgc gcaccaagga cggccatttc atcaggccga cgacgaaatt
     1081 ccgcaattgg gtaacacctg acggcggtcc tggaccgtca ggaaaaggag attttgccgc
     1141 agaggctggc cgctatcatc tctatgtttc gctctcctgt ccgtgggcgc accgcacgat
     1201 cattttccgc aagctgaaag cacttgagaa cgtcgtgtcc atgtcgattg tttcgcccga
     1261 catgctgaag aacgggtgga ccttcaacgt gagcgaggga tcgaccggag acagcgccaa
     1321 cggaaaatca agactgtcgg agatctatct gctcgcggag ccgcgctata ccggccgcgt
     1381 gagtgtgccg gtgctctggg acaagaaaag gaaaacgatc gtcaataatg aatcgtccga
     1441 gatcatccgg acgctcaatt cggcgttcga tgccttcacc aacgtgagga ccgactacta
     1501 tcccgagccg cttcgtgcgc aaatcgacaa attcaacgat ctgatctacc caaacatcaa
     1561 taacggcgtt taccgtgccg ggtttgccac ttcgcaggcc gcctatgagg aggccttccg
     1621 caatgttttc gacacgctcg atgagctcga tcaggttctt tcacagcacc gctatctcgt
     1681 cggaaacacg atcaccgaag ccgactggcg gctgttctgc acgctgatcc gcttcgatgc
     1741 ggtctactat tcgcacttca aatgcaactg gcggcacatc tacgaatatc cgaacctgtc
     1801 gaactacctg cgcgatcttt atcaggtgcc gggcgtagcc gagacggtga gcatcgagca
     1861 gatcaagcgg cactattatg gaagccagcg gcaggtgaac ccgaccggca ttattccggt
     1921 cggaccgcaa ctcgatttcg cggcgcctca cgatcgcgcg cggttcagcg cgtagggctc
     1981 accagacctg gctgaccggc gcacggccga acacttcggc cagacgcgcg cgcgtgcccc
     2041 gcccggtatc ctccggcaat gcatcggggt ggaagaagcc atgcgcgaca atttcgtgat
     2101 tggggcgcgg ctctccgtcc tggcggaaat cacggacgat gaacaaggcg acatggtcgc
     2161 gccgcgacac ccgtttgttg aggtagatgc cgaaaagttg cgggcttgcg ccgagccgga
     2221 tgttgccttc ctcgacgagc tcccgcgcaa gcgcgtcgac gaaggtctct ccggtttcaa
     2281 cgccgccgcc gggaagatgc caaccatcga catatgagtg cttgaccagg aaaacccgtc
     2341 ccgctccgtc gatcaccatg gcgcgtgcgc ccagcgtcgc cggtcgcgag aaacgccagt
     2401 agaagtgcat ggctcggctg atgaccggct caagcagccg gcgcaggcga tgcaagtgct
     2461 ccatccctct ttctgtcacc gatcgccgag accccgtccg agcgcctggc tgctgaaaac
     2521 tcgcagttca ttgtttagaa tgattccaag atatggcata aaacgcgccc tgagcgataa
     2581 cgaggtagag cgtgaaacgg tttgccgatc tgagcgaaca ggaagtcttg gccctcgcca
     2641 tcaccaacga ggaagaggat agccgcatct atcgcggatt tgcggaaggc ctgcgggagc
     2701 aatttccggc ctcggcaaag gtcttcgacg agatggccga tgaggaagtg catcaccgca
     2761 cgatgctgtt cgatctctat cgcaagaaat tcggggacta cctgccgctg atccgccgcc
     2821 aggacgtcaa gggcttcctg catccgaagc aaccgctgtg gctcacgcgg ccgctcggcc
     2881 tcgaagaggt gcgcaaattc gcagagaaca tggaattcga ggcggagcgc tactaccgca
     2941 gggcggcgga caacgcgcgc gacgcctcga tccgacagct gctggtcgag ctcgcggatg
     3001 ccgaagcggg acatgaaagc ctggcgcaca aattgagcga ggagattctc accaagggcg
     3061 cgcgtgccaa ggaagatgaa accgcacggc gcatgttcgt gctgcaatat gtgcagcctg
     3121 gcctcgccgg actcatggac ggctcggtat cgacgctcgc gccgctattt gcggcggcct
     3181 tcgcgacgca caacacctgg gagacctttc tggtcggcat ggcggcctcc gtcggcgccg
     3241 gcatttcgat gggttttgcc gaagcgttgt cggacgacgg ctccctgacc ggccgcggcg
     3301 ctcccctgtt gcggggtacg gtgtgcggcg tgatgacggc gatcggcggg ctcggccaca
     3361 ctttgccctt tctcattccg gacttttacg tcgcgaccgt cgtcgcagtc ctcgtcgtcg
     3421 tggttgaact cgcggtgatc tcgtggatcc gtacccgctt catggacacg cccttcctgc
     3481 aggccgcatt ccaggtggtc gtcggtggcg tactcgtgtt cattgcgggc atcttgatcg
     3541 ggagttcatg atcccgcatc gatgctgacc ctcgcgcatc tttccgaccc acatctcgca
     3601 cctctgccgc agccgcgctg gagcgaactg gccggcaagc ggatgaccgg ttatatcaac
     3661 tggcagagaa agcgccggtt tgttcacgac gcggccgtgc tttcggcgat tgcagccgac
     3721 gtcagggcgc agacgcccga ccacatcgcg gttaccggcg atattgccaa tatagccttg
     3781 ccggctgagt ttctgcgcgg ccgcgaatgg ctcgaaagtc tcggttcgtc gcgcgacgtg
     3841 actttcgtcc ccggcaacca cgacatctac gtgcgcgagg ccggcgctct cgccgatcgt
     3901 caactgagcg catatatgtg cgacgacgac ggtgaaagcg gcttcccgtt tgtcaggcgc
     3961 tgcggcgata tcgcgttgat cgggctctca accggcgtac cgactgcacc gttcctcgcc
     4021 acggggtggc tcggaacgag gcagctcgcc gagttcgcgg cgatcctcaa caacctcaag
     4081 gacgaaaact tttttcggat tatcctgatt caccatccgc cgatgaccaa tgccgcgcgt
     4141 cacaaacggt tgatggatgc ctcgatcctc aa
//
LOCUS       DASSRL010000445         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1691668, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000445 DASSRL010000000
VERSION     DASSRL010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691668"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFL49_09015"
     CDS             <1..854
                     /locus_tag="VFL49_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HET7912160.1"
                     /translation="ANARAGGGFSGGSRGMRTLSAPPVTRTAPNTAAPIQRTVTQPGK
                     AGTIGQAPGRPGLFGGGLFGGLAAGFLGAGLFGLLFGHGFFGGMAGFASILGLVLQVV
                     VIVIVARLLFAWWQRRNMPAPAYAAGHPVTGHSFSGLGGMFGSGAAAAEPLAIGKSDY
                     DVFERLLSEIQAAYSAEDINALRAKVTPEMLSYFSEELAENASRGVVNKVDDVRLEQG
                     DLAEAWREGAVDYATVAMRFSLTDRMIDRASERTVEGGEPAEVTELWTFMRSRGGEWL
                     LSAIQQT"
     gene            complement(876..1208)
                     /locus_tag="VFL49_09020"
     CDS             complement(876..1208)
                     /locus_tag="VFL49_09020"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7912161.1"
                     /translation="MRGDKRKTRRHRLRRNAWFMLDAGQRRECVVTNISDHGAHISVA
                     DSDDSPDSFLLLLAESGATRRRCRVIWRKPREVGVKFDSWLDERIRANVKSKPAVPAE
                     EAKADEKV"
     gene            1519..1722
                     /locus_tag="VFL49_09025"
     CDS             1519..1722
                     /locus_tag="VFL49_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7912162.1"
                     /translation="MPVGTVKFFNTQKGYGFIQPDDGSKDVFVHITAVERAGMRSLVE
                     GQKISFEVVTERGKQAAGNLQPV"
     gene            1861..2262
                     /locus_tag="VFL49_09030"
     CDS             1861..2262
                     /locus_tag="VFL49_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007626202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7912163.1"
                     /translation="MDIIACGSRPSRRMGAPSFTGTVWQDPIIQAPDPARIRSAWVRF
                     EPGARTAWHTHPLGQTLFVTSGIGRVQTWGGPVREIRAGDTVWIPPSEKHWHGATADH
                     ALEHIAMQEAQGGEHVTWLEPVTDAQYSGKS"
BASE COUNT          452 a          703 c          766 g          523 t
ORIGIN      
        1 atgccaatgc ccgtgcaggc ggagggtttt caggcggcag ccggggcatg cggacactct
       61 ccgcgccacc ggtaacgcgc actgcgccta ataccgccgc gccaattcag cgaactgtga
      121 cgcagccggg taaagccggc acaatcggcc aggcgccagg acgacccggc ctctttggcg
      181 gcgggttgtt tggcgggctt gcggcgggtt ttctcggcgc tggtctcttt ggcctgctgt
      241 ttgggcatgg atttttcggt ggcatggccg gcttcgcatc catcctcggc ctcgtgctgc
      301 aggttgtcgt gatcgtgatc gtggcgaggt tgctgttcgc gtggtggcag cgcaggaata
      361 tgccggcgcc cgcctacgcg gccgggcatc ctgtcaccgg acactccttc agcgggctcg
      421 gcggcatgtt cgggtccggt gctgcggccg ccgaaccgct ggcaatcggc aagtccgact
      481 acgacgtctt tgagcggctg ctcagcgaaa ttcaggctgc ttattccgcg gaagacatca
      541 acgcgttgcg ggccaaggtg acgccggaga tgctctcgta cttttcggaa gaactcgctg
      601 agaatgcaag ccgtggcgtt gtcaacaaag ttgatgacgt gcggctcgaa cagggcgatc
      661 ttgcggaggc ctggcgcgag ggcgcggtcg actatgcaac cgtggccatg cgcttttcgc
      721 tgaccgatcg catgatcgac cgggcaagcg agcgcactgt cgaaggtggc gagcctgccg
      781 aggtcacgga attgtggacc ttcatgcgct cacgcggcgg cgaatggctg ctttcagcta
      841 tccagcaaac gtgagggcgt cgatcaaggc agccgtcaga ccttttcgtc cgctttggct
      901 tcctctgcgg gtacggcggg ttttgatttg acgttggcgc gtatccgttc gtcgagccat
      961 gagtcaaatt tcacgccgac ctcgcgcggt ttgcgccata tcacccggca gcgccgccgc
     1021 gtcgcgccgc tttcagccag cagcaacagg aaactgtcag gactatcgtc cgaatcggcg
     1081 acgctaatat gagcgccgtg gtccgagata ttggtgacca cgcactcacg gcgctgaccc
     1141 gcatcgagca tgaaccatgc gttgcggcgc aatcggtgtc gtctggtctt tcgcttgtcc
     1201 cctcgcatgt cgcctcgcag gcgtcggatt tagaacattc agattctgtt aattgttgcc
     1261 ggttggtgag gcgctaccgt aacaactagc ctttacgaat tcataaagcg ttatccgacg
     1321 ccgccgattg cgctccgaac cgttgacgtt gcactgaaat ccgtctatgt ggccgcccgc
     1381 aacctggttt tcggcctcaa cttgctgcca cgctcctggt tttgggctgc gctcaagtga
     1441 actcccaaaa acttggcttg tgatcgccgt cgcgataacg cgcaggcgtt caacaggaag
     1501 cgtgaaagga aactcttgat gcctgtcgga actgtcaaat ttttcaatac acaaaagggc
     1561 tacggcttta ttcagccgga tgatggctcg aaggatgtct ttgtgcatat cacggcggta
     1621 gagcgcgccg gaatgcgctc gctggtcgag ggccagaaga tcagctttga ggtcgttacc
     1681 gagcgcggca aacaggccgc cggcaatttg cagccggtct gagcgtcacg gattgccctg
     1741 aaatcgaagc ccggcggaaa cgccgggctt ttttgcatcc gacacattta gagtgctgct
     1801 tttaaagtat tgcattcgaa caggcagatc aggacactga gacgacgagg agaaacgaca
     1861 atggacatca tcgcttgcgg atcacgcccg tcgcgtcgca tgggcgcacc gagcttcaca
     1921 ggaaccgttt ggcaggatcc gatcatccag gcgcccgatc cggcacgtat ccgatccgcc
     1981 tgggtgcgct tcgaaccggg tgcacgcacg gcttggcata cgcatccgct cggacagact
     2041 ctctttgtaa cgtccggcat cggtcgcgtg cagacctggg gtggaccggt tcgcgaaatc
     2101 cgtgccggag acacagtctg gattccgcca agtgaaaagc attggcatgg agcgaccgcc
     2161 gatcacgcgc tcgaacatat tgcgatgcag gaagcccaag ggggcgagca tgtcacctgg
     2221 ctcgaaccgg tgacggacgc gcagtattca ggcaaatcct gaacttgatt tgcgtcatca
     2281 aggacgcgtg atcgcaggaa tacagcgtcc gcggtggtgt ttggtcctcg cgatggtggg
     2341 gacgtcgggt ctccgccgat tactcgggag gtatccgatg acggttgttc gaattggcgc
     2401 cccactggcg gcgtgcgtga tgcttttttg cggggctcct gcct
//
LOCUS       DASSRL010000446         7820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_29082, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000446 DASSRL010000000
VERSION     DASSRL010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29082"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="ilvN"
                     /locus_tag="VFL49_09035"
     CDS             <1..498
                     /gene="ilvN"
                     /locus_tag="VFL49_09035"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7912164.1"
                     /translation="KAETHTLSVLVDNEPGVLARVIGLFSGRGYNIESLTVSETEHRQ
                     HLSLITIVTTGTPMVIEQIKNQLDRLVPVHHVVDMTLAGNAIERELAMVKVKGKGDNR
                     VEALRLADAFRARVIDATTESFVFEITGASEKIEQFIALMLPLGLVEVARTGIAAIMR
                     GPEGM"
     gene            501..731
                     /locus_tag="VFL49_09040"
     CDS             501..731
                     /locus_tag="VFL49_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912165.1"
                     /translation="MGLTILSIPLIALGVFLKPYSLENSRCFNVSFLRAQPYCFAQDS
                     QMPEIIKYGSVIAGFALLYAGRRQIRRAREKE"
     gene            complement(737..1468)
                     /locus_tag="VFL49_09045"
     CDS             complement(737..1468)
                     /locus_tag="VFL49_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HET7912166.1"
                     /translation="MSQQTYPPLNTLKPVDNEIWIVDGDLIRFGPPGFRFSFPTRMTI
                     IRVGTALFIHSPTELTAGLKAEIDRIGAPTWLIGPNRLHYWWIPDWRAAYPSARVYLA
                     PRIKEQAGPRIDFRADELTENAGYPWASAIATLAVPGSYMTEFVFFHHATRTLVLTDL
                     IENFEPRKLGPFERWLTRIGGVRDPDGKMPLDMRLTFFRRKAQLRSAVETMIAWQPDR
                     IVLAHGRWYTTGGVNELRRAFRWLL"
     gene            1579..2598
                     /gene="ilvC"
                     /locus_tag="VFL49_09050"
     CDS             1579..2598
                     /gene="ilvC"
                     /locus_tag="VFL49_09050"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7912167.1"
                     /translation="MRVYYDRDADLNLIKGRKVAIIGYGSQGHAHALNLRDSGVKEVA
                     VALRKTSAGVKKAEAEKFKVMDVAEAAKWADVMMMLTPDELQGDIYRDHLHKNMKDGA
                     ALMFAHGLNVHFNLIEPRADLDVLMIAPKGPGHTVRSEYQRGAGVPCLVAIHKDASGN
                     AHDLGLSYASAIGGGRAGIIETTFKEECETDLFGEQTVLCGGLVELMKAGYETLVEAG
                     YAPEMAYFECVHEVKLIVDLIYEGGIANMNYSVSNTAEYGEYVSGPRVITPEVKAEMK
                     RVLSDIQSGRFTKDWMLENKVNQTSFKATRAKLAEHPIEQVGAKLRDMMPWIKQRALV
                     DKSKN"
     gene            complement(2595..4142)
                     /locus_tag="VFL49_09055"
     CDS             complement(2595..4142)
                     /locus_tag="VFL49_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET7912168.1"
                     /translation="MALDARLAEAWSLLPDYLGSHVLVSLAALAIGLGISLPVAVASR
                     NRPILRGVLLAIASVVQTIPGLALLALFYPLLLALSALSERVFGKGFSALGFLPAVLA
                     LALYSMLPVLRNTVTGLHGIDARLSEAARVVGVRPWQLLRDIELPLAMPVIMAGIRTS
                     AVWVIGTATLATPIGQTSLGNYIFSGLQTQNWVFVIFGCVAAALLALAVDQLLALVQA
                     GATRRSSARTAVGVAGLAALTLAALIPGLARPHADYIIGAKTFTEQYILAALIEQRLQ
                     AAGLGAERRPGLGSNVLFDALAKNEIDVAVDYSGTIWTNRMRRTDTPPRPQLLAELEK
                     WLSERHGIRVLGTLGFENAYALAMTRKKAQALGIRSIADLAAHAGELTIAGDYEFFVR
                     PEWDALRKAYGLQFKAQRQMQPEFMYKAVVANDVDVISAYTSDGQIALNDLVVLDDVR
                     RAIPPYDAILVLSPRRAHDEKLISAMKPLIGAIPVDLMRQANARASGGVSPDAVARWL
                     GEKLGLN"
     gene            4425..6053
                     /locus_tag="VFL49_09060"
     CDS             4425..6053
                     /locus_tag="VFL49_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7912169.1"
                     /translation="MVALLHVAALIIMVATEVDPVAKIAFLLTWALLNFACLVLVRRP
                     IASALISLSFIVALILLSQFKHEKLWMTVDFVDLMIIDRDTSSFLLAVIPGLRTQLML
                     AAFIVATLLAIAWRLDPLRMRLRMSALAGTFCLAALTVLSLSFPTDLYEDFFGHNYVS
                     KFARTGVEAVHELITHGYLDSDATITERLKAPPIATCAPARKLPHIILLHDESSFDIT
                     AIPGVSVPPGYKNHFRSFDGKVRKLIVEGAGGPSWFTEYNVLTGLSSRSYGRFATSVT
                     RIAAGRVGRGLPASLTACGYKTFSLYPFYGAFLSSRAFQTTAGIGKYLDMADLGTENF
                     ERDSFYFGQAEKIIARERGHGPLFLYVYTVANHFPWDSRLRPELTPNWRELGNAPNVD
                     EYIRRQGMTAQDYAALLDRLKRDFPSEAFLIVRYGDHQPEFAHLMIDPGLSDSARARQ
                     MKANDPRYFTSYYAIDTVNFRPADMSSALPTLEAPHLPLLVQEAAGVPLDSSFAEQKK
                     ILQRCHGAFYGCAGGEEARRFNRLLIEAGLIKGL"
     gene            6228..7058
                     /locus_tag="VFL49_09065"
     CDS             6228..7058
                     /locus_tag="VFL49_09065"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912170.1"
                     /translation="MAHGTAVASKNAGAAVALTDVVVSFRVAIGTYTAVDRATLNVGD
                     GEFVAIVGPTGCGKSTLLNIAAGLLAPADGSVNIFGAPLSGLNRQAGYLFQADALFPW
                     KTAIENVAIGLETAGTPTKEARSRAQNWLARVGLGTFADRYPHMLSGGQRKRVGLAQV
                     LIRDPKILLMDEPFGPLDAQTRQIMGNLLLDLWTADRKAVLFVTHDLEEAIALSDRVV
                     IMSAGPAARIIGDWNVPIARPRDIAEIKLDPGFVALHREIWQKLKTEVIKGYAQSEAV
                     "
     gene            7125..7676
                     /locus_tag="VFL49_09070"
     CDS             7125..7676
                     /locus_tag="VFL49_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7912171.1"
                     /translation="MKKLTLSAAMLTLLGTAAFAADPFTLSSSSFKDGTMMDVKHAGN
                     IKANPNCVGQNVSPELKWTNAPAAAKSFVLVASDWEGQGGLGSHHLLAYNIKPTVTAF
                     AEGELSKDSDKFTGGKETAGTLHYRGPCPPAGTAVHHYVFTVIASDLEPGALPAGLTR
                     DELWEKLKGHAKGVAGIVGLFKR"
     gene            7690..>7820
                     /locus_tag="VFL49_09075"
     CDS             7690..>7820
                     /locus_tag="VFL49_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912172.1"
                     /translation="MAQIKLTALQVLVAIVAVVLWHVLTTYPVFGKILLPPFFFSNPV
                     "
BASE COUNT         1516 a         2408 c         2415 g         1481 t
ORIGIN      
        1 aaagcggaaa cccatacgct ttccgttctg gttgacaacg agcctggcgt gcttgcacgc
       61 gtgatcggtc tgttttccgg ccgcggctac aatatcgagt cgctgacggt gtcggagaca
      121 gagcacaggc aacatctgtc gctgatcacg atcgtcacca ccggcacccc aatggtgatc
      181 gagcagatca agaaccaact cgaccggctc gtcccggtgc atcacgtcgt cgatatgacg
      241 ctcgccggaa atgcgatcga gcgcgagctt gcgatggtca aagtgaaagg aaaaggcgac
      301 aaccgcgtcg aagctttgcg gcttgcagat gcatttcgcg cgcgagtgat cgacgccacc
      361 accgaaagct tcgtgttcga gatcaccggc gccagcgaaa agatcgagca gttcatcgcg
      421 ctgatgctgc cgctcggtct tgtcgaggtt gcccgcacgg gtatcgccgc catcatgcgg
      481 ggaccggagg gaatgtaacg atggggctga cgattctgtc gattccgctt attgctctcg
      541 gcgtttttct gaaaccgtat tccctggaga attcgcgttg cttcaacgtc agctttctgc
      601 gggcgcagcc ttactgcttc gcgcaggact cgcagatgcc cgaaatcatc aaatacggca
      661 gcgtaattgc cggcttcgct ctgctttacg ccggcagacg ccaaatcagg cgcgcgcgcg
      721 aaaaagagtg atattttcag aggagccaac ggaaagcgcg gcgcaattcg ttgacgcctc
      781 cggttgtgta ccaacgccca tgcgccagaa cgatacgatc aggttgccag gcaatcatcg
      841 tttccacggc gctgcgcagc tgcgctttac gccggaagaa tgtcaagcgc atatcgagcg
      901 gcatcttgcc atcgggatcc cggacgccgc cgatccgtgt cagccagcgc tcgaaagggc
      961 cgagcttccg cggttcgaaa ttctcgatga ggtccgtgag gaccagcgtg cgggttgcat
     1021 gatgaaagaa tacgaattcc gtcatgtagc tgccggggac ggcaagcgtc gcgatcgcgc
     1081 tggcccacgg atatcccgca ttttccgtga gctcatccgc acgaaagtcg atgcgcggac
     1141 cggcctgttc tttgatgcgg ggcgcgaggt agacgcgcgc actcggatag gcggcgcgcc
     1201 agtccggaat ccaccagtaa tgcagccggt ttggtccaat gagccaggtt ggcgcgccta
     1261 tgcggtcaat ttccgccttc aggcccgcgg ttaactccgt cggcgaatga ataaacaatg
     1321 ccgtgccgac acgaatgatc gtcatccgtg tcgggaagga aaatcggaag ccaggcggtc
     1381 cgaagcggat aaggtcgccg tcgacgatcc agatttcgtt gtcgacgggc tttaacgtat
     1441 taaggggcgg ataggtctgc tgactcattg aggaccaaag gaaaatggta gcgtttcgtt
     1501 ccctagcagt ttcccgcttg attttcccgg gccaatgccg tctttgcgct gtgaagaagc
     1561 tggaaggggt taagtaccat gcgtgtttac tacgatcgtg acgccgacct gaacctcatt
     1621 aaaggaagaa aggtcgccat catcggctat ggaagccagg gccacgccca cgcgctcaat
     1681 ctgcgcgaca gcggcgtgaa agaggtcgcg gtcgcattgc gcaagacctc cgccggcgtg
     1741 aaaaaggccg aggccgagaa gttcaaggtc atggacgtgg ccgaagccgc caaatgggcc
     1801 gacgtgatga tgatgctcac gcccgatgaa ttgcagggcg acatttaccg cgaccatctg
     1861 cacaagaaca tgaaggacgg cgcggcgctg atgttcgcgc atggcctcaa cgtccacttc
     1921 aacctgatcg agccgcgcgc cgatctcgac gtgctgatga tcgcgccaaa aggtcccggc
     1981 cacaccgtgc gctcggaata tcagcgcgga gcgggcgtgc cgtgcctggt cgcgatccac
     2041 aaggacgcct cgggcaatgc gcacgatctc ggtctttcct atgcctccgc gatcggcggc
     2101 ggccgcgcgg gcatcatcga gaccacgttc aaggaggagt gcgagaccga tctgttcggc
     2161 gagcagaccg ttctttgcgg cggcctcgtc gagttgatga aggcgggcta tgagacgctg
     2221 gtcgaagccg gctacgcgcc ggagatggcc tatttcgagt gcgtgcacga ggtgaagctg
     2281 atcgtcgacc tcatctacga gggcggcatt gccaacatga attattcggt gtcgaacacc
     2341 gccgagtacg gcgaatacgt atccggcccg cgcgtgatta cgccggaagt gaaagccgaa
     2401 atgaagcgcg tgctgtccga catccagtcc ggacgtttca caaaagattg gatgctggag
     2461 aataaggtca accagacctc gtttaaggcg acgcgcgcca agctcgccga gcacccgatc
     2521 gagcaggtgg gcgccaagtt gcgtgacatg atgccctgga tcaagcaacg cgcgctggtg
     2581 gacaagagca aaaactagtt cagtcccagc ttctctccta gccaacgcgc caccgcgtcc
     2641 gggctgacac cgcccgacgc gcgcgcattg gcttggcgca taagatcgac agggatcgct
     2701 ccgatcaggg gcttcatcgc actgatcagc ttttcatcgt gcgcacgccg gggtgagagc
     2761 acgaggatcg catcgtaagg gggaatggcg cgcctcacat cgtcaagcac gacgaggtcg
     2821 ttgagcgcaa tttgaccgtc gcttgtgtaa gccgagatca catccacgtc gttggcgacg
     2881 acggccttgt acatgaattc cggttgcatc tggcgctgcg ccttgaactg caggccatag
     2941 gccttgcgca gcgcgtccca ttcgggccgc acgaagaact catagtcgcc ggcgatggtg
     3001 agttcgcccg catgcgcagc caggtctgcg atcgagcgaa tgccgagtgc ctgggccttt
     3061 ttgcgtgtca tcgcgagcgc ataggcattc tcaaatccaa gcgtgcccag cacgcggatg
     3121 ccgtgtctct cgctcagcca cttctccagt tcggcaagca gctgcggccg cggcggcgta
     3181 tccgtgcgcc gcatacggtt cgtccagatg gtgccggaat aatccaccgc gacatcaatt
     3241 tcgtttttgg cgagggcatc gaacagaaca ttggagccca gccccggccg gcgctcggcg
     3301 ccgaggcctg cggcctgaag acgctgctcg atcagcgccg caagaatgta ttgttcggtg
     3361 aaggtcttgg cgccgatgat ataatccgcg tgcggccgag cgagaccagg tatcaatgca
     3421 gcaagtgtca gcgcggcaag gcctgcaacg ccgacagccg tccgtgcact gctgcggcgg
     3481 gtggcgccgg cctgcaccag ggccagcagt tgatcgacgg caagcgcgag gagcgctgcc
     3541 gcgacgcagc cgaaaattac gaacacccag ttctgggtct gcaggccgga gaaaatatag
     3601 ttgccgaggc tggtctggcc gatcggggtt gcgagcgtcg cggtgccgat cacccagacc
     3661 gcggacgttc gtatgcccgc catgatcacc ggcatcgcca gcggcagttc gatgtcgcgt
     3721 aacagctgcc agggccgcac gccgacgacc cgggcggcct cgctcaggcg ggcatcgatc
     3781 ccgtgcaggc cggtcacggt gttgcgcaga accggcagca tcgaataaag cgcgagcgcc
     3841 agcactgccg gcaggaaacc gagcgccgaa aatcctttgc cgaacacacg ctcggaaagg
     3901 gcgctcaacg ccagcagcag ggggtagaac agggccagca gcgcgagccc gggaatggtc
     3961 tgaacgacgc tggcaatggc cagcaggacg ccgcgaagaa tgggccggtt tcttgaagcg
     4021 accgcgaccg gcaggctgat gccgagcccg atggcaagcg cggccaggct caccagcaca
     4081 tggctgccga gatagtcggg caggagcgac catgcctcgg caagacgggc gtcgagggcc
     4141 atgcaggcga tgatggcatg cgcgacgcag cggcggaacc gggcatttcg tgagctggcc
     4201 gtccatcccc ttggttaaca tgacaaaatt tcagccgtgt cgctgccggc cgcttgcaat
     4261 gacagtgtga ttacagtctt atgacggcgg gccgtgcgct ggagaccgca agaggatcgc
     4321 agccgggcag ggccgcctag gccatgttgt ccgcagcgtc cgcactgctc agtcgtctcg
     4381 cgcccgttga agggatttcg cgccgcgagg cgctggcaat atccttggtt gcactgctcc
     4441 acgtcgccgc actcatcatc atggtggcga ccgaagtcga tcccgtcgcg aaaatcgcct
     4501 tcctgctcac ctgggccttg ctcaatttcg cctgtctcgt ccttgtgcga cgcccgattg
     4561 cgtctgctct catctcgctc tcgttcattg tagcgctgat cctgctctcg cagttcaagc
     4621 acgagaagct ctggatgacg gtcgacttcg ttgatctgat gatcatcgat cgcgatacct
     4681 catccttcct gctggcggtg atcccggggc tgcggacgca gctgatgctc gctgcattca
     4741 ttgtggcgac gctgcttgcg atcgcgtggc gcctcgatcc gctgcgcatg cggttgcgca
     4801 tgagtgcgct cgccggaaca ttttgccttg ccgcgctcac ggtgctgtcg ctgtcgttcc
     4861 ccaccgatct ctacgaggat ttcttcggcc acaactacgt ttcgaaattc gcgcgcaccg
     4921 gtgttgaggc ggtgcacgaa ttgatcacgc acggttatct tgactccgat gcgaccatca
     4981 ccgagcgtct gaaagcgccg ccaattgcca cgtgcgctcc ggcgcgcaag ttgccgcaca
     5041 tcattctcct gcacgatgaa tcgagctttg acatcaccgc catcccgggc gtcagcgtgc
     5101 cgcctggcta caagaaccat ttccgttcgt ttgacggcaa ggtacgcaag ctgatcgtgg
     5161 agggtgcggg cggcccgagc tggttcacgg aatacaatgt gctcaccggc ctttcctcac
     5221 gctcctacgg tcgcttcgcg acctcggtca cgcgcatcgc cgccggccgt gtcgggcgcg
     5281 gattgccggc gtcgctgacg gcgtgcggct acaagacctt cagcctctat ccgttctacg
     5341 gcgctttcct gagttcgcgc gctttccaga ccacggccgg tatcggcaaa tatctcgaca
     5401 tggccgacct cggtacggag aatttcgagc gcgacagttt ctacttcggc caggcggaaa
     5461 agatcatcgc gcgtgagcgc gggcacgggc cgctcttcct ctacgtctat accgtcgcca
     5521 atcattttcc ctgggacagc cgcctgcggc ctgaactgac gccgaactgg cgcgagctcg
     5581 gcaacgcgcc caatgtcgac gaatacatcc gccgtcaggg catgaccgcg caggactatg
     5641 cggcgctgct cgatcgtctg aagcgcgact tcccctccga agcgttcctg atcgtgcgct
     5701 acggcgacca ccaaccggaa ttcgcgcatc tcatgatcga cccgggcctc agcgatagcg
     5761 cgcgtgcgcg ccagatgaag gccaacgatc cacgctattt caccagctac tacgcgatcg
     5821 acacggtgaa tttcagacca gccgatatgt catccgcact gccgacgctg gaggcgcctc
     5881 atctgccgct cctggtgcag gaggcggccg gcgtgccgct cgattcgtct ttcgccgagc
     5941 agaaaaagat cctgcaacgc tgtcacggcg cgttctacgg ctgcgccggc ggcgaggagg
     6001 cacgccgttt caatcggctg ctgatcgagg cggggctgat caagggcctg tagcgctttt
     6061 ctgactctcc ttcgtcatgg ccgggctggt cccggccatc cacgtcttcc ggtcggcaaa
     6121 gacgtggatg cgcgggtcaa gcccgcgcat gacggatgtg gacgcctttg caatccggca
     6181 tgacccagat aggttgcccc aataaaagcg aggcgggagg aaacggcatg gcgcacggca
     6241 cagccgtcgc atcgaaaaat gctggcgcgg ccgttgcgct caccgacgtc gtcgtctcgt
     6301 ttcgcgtcgc catcggcacc tacacggcgg ttgaccgtgc aacgctgaac gtcggcgatg
     6361 gcgagttcgt cgccatcgtc gggccgaccg gctgcggcaa gtcaacgctg ctcaacatcg
     6421 ctgccggtct tctggcgccc gccgacggca gcgtgaacat tttcggcgcg ccgctttcgg
     6481 ggctcaaccg tcaggccggt tatctgtttc aggcggacgc cctctttccc tggaaaaccg
     6541 ccatcgaaaa cgttgccatc ggcctcgaga ccgccggcac gccgacgaaa gaagcgcgct
     6601 cgcgcgcaca aaattggctc gcgcgtgtcg gcctcggcac cttcgccgac cgctatccgc
     6661 acatgctgtc cggcggtcag cgcaagcgcg tcggtcttgc ccaggtgctc atccgcgacc
     6721 cgaaaatact gctgatggat gagcccttcg gcccgctcga tgcccagacg cggcagatca
     6781 tgggcaacct gctgctcgac ctctggactg cggaccgcaa ggcagtgctt tttgtcacgc
     6841 acgacctgga agaggcgatc gcgctttccg accgggtggt gatcatgtcg gccggtccgg
     6901 ccgcgcgtat catcggcgac tggaacgtgc cgatcgcgcg cccgcgcgac attgccgaga
     6961 tcaagctcga tcccggcttc gtcgcgctgc atcgtgaaat ctggcagaag ctcaagaccg
     7021 aggtgatcaa gggttacgcg caaagcgagg ccgtgtgagt caaggaattt tgtgtgaatg
     7081 tcgttgttaa caagactgca caagcaaaag ggggaggatt ccaaatgaag aagctgacac
     7141 tgtccgccgc tatgctcact ttgctcggca cggccgcctt tgctgccgat ccgttcacgc
     7201 tgagctcatc gagcttcaag gacggcacga tgatggacgt gaagcacgcc ggcaacatca
     7261 aggccaaccc gaactgcgtc gggcagaacg tatcgcccga actcaaatgg acgaatgcgc
     7321 cggccgccgc caaaagcttc gtgctcgtgg cgtccgactg ggaggggcag ggcgggctcg
     7381 gctcgcacca tctgctcgcc tataacatca agccaacggt cacggccttt gccgaaggcg
     7441 agttgagcaa ggactctgac aagttcaccg gcggcaagga aaccgcgggc actctgcatt
     7501 atcgtgggcc atgcccgccg gccggcaccg ccgtgcatca ttatgtcttc acggtcatcg
     7561 ccagcgacct cgagcccggc gcgctgcctg cggggctcac ccgcgatgag ctgtgggaga
     7621 agctgaaagg gcacgcgaag ggggttgcgg gaatcgtcgg gctgttcaag cgctagccgc
     7681 acggtggtta tggcccagat caaactcacg gcgctgcagg ttctggtcgc aatcgtcgcg
     7741 gttgtgctgt ggcacgtgct cacgacctat cccgtgttcg gtaagattct tctgccgccg
     7801 ttcttctttt cgaacccggt
//
LOCUS       DASSRL010000447         5693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_134250, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000447 DASSRL010000000
VERSION     DASSRL010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134250"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..588)
                     /locus_tag="VFL49_09080"
     CDS             complement(217..588)
                     /locus_tag="VFL49_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7912173.1"
                     /translation="MPKTVLIVEDNELNMKLFHDLLEAHGYQTVGTRNGIEALDLARK
                     HQPDLILMDIQLPEVSGLEVTKWLKDDPELKAIPVVAVTAFAMKGDEERIREGGCEAY
                     LSKPISVGKFIETIRHFLGAA"
     gene            641..973
                     /locus_tag="VFL49_09085"
     CDS             641..973
                     /locus_tag="VFL49_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HET7912174.1"
                     /translation="MPISYGKANWPVKQGSRFARKDAEALAIQALTFIAGDGERLGRF
                     LAVTGIGPDEIRAAAREPGFLGGVLDYIVSDERLIADFVSEADLDPAEIERGRAMLAG
                     NPWERDVP"
     gene            970..2217
                     /locus_tag="VFL49_09090"
     CDS             970..2217
                     /locus_tag="VFL49_09090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7912175.1"
                     /translation="MSFCRDCLSDAPDSASRCPACRSPRLARHPELHTLAIAHIDCDA
                     FYATIEKRDNPDIADKPVIVGGGTRGVVAAACYVARTYGIRSAMPMFEAKRRCPDAVV
                     IKPNMAKYVQVGREVRKAMFALTPLVEPLSIDEAFLDLTGTERLHGKSAAKALATFAT
                     RVERDFGITVSIGLSANKFLAKIASDLDKPRGFAVLGTADAVSFLASKPVGFIYGIGA
                     VSAAKLAADGFRAIADLQHADERNLMLRYGEEGSRLWRLARGQDARPVNPERDTKSIS
                     AETTFNSDISEFRPLEQYLWDLTERVSGRLKANALAGSTVTLKLKSADFKIRTRARSL
                     GAATQLASRIFAAGRDLLTHEVGTTRFRLIGIGVSNLEDAVGDDLADLIDRRAADVEH
                     AVDRLRLKFGRNAVVKGLALDDN"
     gene            complement(2226..3101)
                     /locus_tag="VFL49_09095"
     CDS             complement(2226..3101)
                     /locus_tag="VFL49_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HET7912176.1"
                     /translation="MTMRIPGGTRTWLAVGFGIVVMAAGQPASAAPPVDRVLLAPHRA
                     VYDLKLAKSRGSRGIEDVRGRILYDFSGSACEGYQLQFRQVSELDSGEGKAALSDLRS
                     TTWEDGEAKTFRFNSENLFNERRTDIVDGHAERNEKAVVVSLSKPHERSFTVPATAVF
                     PTEHMRRIIAAAREGTSVLEFPVYDGSESGEKLYNTLTVIGHPIEPGAKVPNDAGAKI
                     PALASLTRWPVTISYFDKTDKKSEESGEEMPVYSITFELYENGISRSLFLDYTDFTIS
                     GEMTSLEMKNSKPCP"
     gene            3264..3731
                     /locus_tag="VFL49_09100"
     CDS             3264..3731
                     /locus_tag="VFL49_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7912177.1"
                     /translation="MSGSVEKKLANLGISLPTPATPIANYVPFVRSGNMLVISGQLCF
                     GVDGNLVAKGQLGGSVSMDDGQKAARACAVNLLAQLKAALGDLDKVTRVIRLGGFINS
                     APGFTDGPKVMNGASDLMVEVFGEKGRHARSTVGVAALPANAAVEVEGQFEVS"
     gene            3739..4482
                     /locus_tag="VFL49_09105"
     CDS             3739..4482
                     /locus_tag="VFL49_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET7912178.1"
                     /translation="MSLDWLTARPVAHRGLHDGQRGIIENTATAFSAALSNNYGIETD
                     LQLSADGEAMVHHDDALGRLTEGSGRLDALSAAQLKSIRFKATADRILTLGELLDLVA
                     GRVTLLLELKSHFNGDDRLLARTANVLSAYRGAAAIMSFDPALTELARHRFPALVRGI
                     VAQRHYPQSEWKVLSAAARWNMGLLLHVPQSRPQFIAYSVRDLPAGPPLLMRALWKLP
                     LLTWTVRNDEDRKRAARWADQVIFEGWRP"
     gene            4547..4828
                     /locus_tag="VFL49_09110"
     CDS             4547..4828
                     /locus_tag="VFL49_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912179.1"
                     /translation="MARLLTPIIIIAAFSGVICAAAADPEGLFDITLSAASKKSTSAK
                     RPQTGQIACTVTGCQRIPPNCRPETGYNWDGIPTGFDIVVCRYPRGRRG"
     gene            4885..>5693
                     /locus_tag="VFL49_09115"
     CDS             4885..>5693
                     /locus_tag="VFL49_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HET7912180.1"
                     /translation="MTDFELRIRITQSLADVPARAWNMCADGILAPAVQPKSELESTS
                     GENLSSTLSTRRIPSNPFVSQEFLSALEASGSVGGRTGWQPRHLLAEDTAGELVGVAP
                     CYVKMHSRGEYVFDHGWAEAFERAGGNYYPKLQVAVPFTPVTGPRLLVRPGLQTDAVR
                     GALADALVEITTASDLSSAHVTFPTEDEWHALGQRHFLRRTDQQFHWENADYKSFDDF
                     LAALASRKRKTIRRERKEALTASIEVRWLTGADLTESVWVAFFALYMETGSR"
BASE COUNT         1150 a         1716 c         1654 g         1173 t
ORIGIN      
        1 acgtatcatt catggcttgc gagaacacgc tcgaatcgat ggcgcgcaac gaaggcaagg
       61 aaccgccgct cgtcgagaac gccgaacgcg taaaggcggg cgtcgtcacc ctgatcgagc
      121 tcggagaaaa gggctggacg atcgtccgtc cctgagcggc acgaattttc gcaacggttc
      181 ggattatctg ctgtggataa cttatggcag ccttcgtcac gccgcgccaa gaaaatgacg
      241 aatggtctcg atgaatttcc cgaccgatat cggctttgac aaataagcct cgcacccgcc
      301 ctcgcggata cgttcttcat cgcccttcat ggcaaacgcg gtgaccgcca ccaccggtat
      361 cgctttgagc tcgggatcgt ccttgagcca ctttgtgact tcaagtccgg atacttccgg
      421 caattgaatg tccatcaaaa tcaggtcggg ctgatgcttg cgcgcgagat cgagcgcctc
      481 aatgccgttg cgcgtcccca cagtctggta accgtgagcc tcaaggagat cgtggaagag
      541 cttcatattg agttcattgt cctccacgat caggacggtc ttcggcattc tatttccgtc
      601 cttttgcgcc ccgccatagt cgggcatcat cggcaagcta gtgccgattt cttacggaaa
      661 ggcaaattgg ccggtgaaac aaggctcccg attcgcacgt aaagacgctg aagcgcttgc
      721 gattcaggcc cttacattta ttgccggcga cggcgagcgg cttggccggt tcctcgcagt
      781 caccggcata gggcctgacg agattcgcgc cgccgcgcgc gagccgggat ttctgggcgg
      841 cgtcctcgac tatatcgtat ccgatgaaag gctgatcgcc gatttcgtat ccgaggcgga
      901 cctcgatccc gctgaaatcg agcgcggccg cgcaatgctc gcgggcaatc cctgggagcg
      961 ggacgtgcca tgagtttctg ccgcgattgt ctcagcgacg cgccggatag cgcgagccga
     1021 tgcccggcgt gccggtcccc gcgcctcgca cgacatccgg agttgcacac gctggcgatt
     1081 gcccacatcg actgcgatgc cttctatgcg accatcgaaa aacgcgacaa tcccgacatt
     1141 gccgacaagc ccgtcattgt tggcggcggc acgcgcggcg tcgtcgccgc ggcgtgttac
     1201 gtggcccgca cctacggtat ccgttcggcg atgccgatgt tcgaggccaa acggcgatgt
     1261 ccggatgccg ttgtcataaa accgaacatg gcgaaatatg ttcaggtcgg gcgtgaagtg
     1321 cgcaaggcga tgttcgctct gacgccgctc gttgagccct tatcgattga tgaagctttt
     1381 cttgacctca ctggcacaga acggctgcac ggaaaaagcg cggcaaaggc tttggcaacc
     1441 ttcgcaaccc gggtcgaacg cgacttcgga attacggtat cgatcggact gtcggcgaac
     1501 aaattccttg ccaaaattgc ctcggatctc gacaagcccc ggggctttgc agtgctcggc
     1561 accgcagatg cggtttcgtt tctcgcgagc aagcccgtcg gcttcatcta cgggattggc
     1621 gcggtaagtg cggcgaagct tgcggccgac ggtttccgag caatcgcaga tctgcagcac
     1681 gccgatgaac ggaacctcat gctgcgctat ggcgaggaag gctcgcgact ctggcggctc
     1741 gcgcggggac aggacgcgcg accagtgaac ccggagcgcg acaccaaaag catttctgcc
     1801 gaaacgacct tcaatagcga catcagtgaa tttcgtccgc tcgagcaata cctgtgggat
     1861 ctgaccgagc gggtctcggg acgtctcaag gcaaatgcgc ttgccggatc gactgtgacg
     1921 ctcaaactga agagtgccga cttcaagatc cgcacaagag cgcgctccct tggcgccgcc
     1981 acccagcttg cgtcacgaat atttgccgcc gggcgtgacc tgctcacgca cgaggtgggc
     2041 accacgcgct tccgcctgat cggaatcggc gtcagcaatc tggaggatgc cgtgggtgac
     2101 gaccttgccg acctgatcga ccgacgggcg gcagatgtcg aacacgcggt cgacagactg
     2161 cgcttgaagt tcggacgtaa cgccgtggtg aaaggcctgg cgctcgacga caattagccg
     2221 ccgcgtcacg ggcagggctt cgaattcttc atttccagcg acgtcatctc gccgctgatg
     2281 gtgaagtccg tgtagtcgag gaagagcgag cgtgaaatcc cgttctcgta aagctcgaac
     2341 gtaatcgagt agacgggcat ctcctccccg ctttcctccg actttttatc cgttttgtcg
     2401 aaatagctta tcgttacagg ccagcgcgta aggcttgcca atgctggaat ctttgcacca
     2461 gcgtcgttcg gcaccttggc gcccggttcg atcggatgac caataacggt gagcgtgttg
     2521 tagagtttct ctccgctttc cgatccgtca tagacgggaa actccaagac cgacgtacct
     2581 tcacgcgccg cggcaataat ccgtcgcata tgttcggtgg gaaagaccgc ggtagccggc
     2641 accgtaaaac ttctctcatg aggcttactc aggctgacga cgaccgcctt ctcattgcgt
     2701 tcggcgtgcc catcaacgat atcagtgcgc cgctcattaa acagattttc cgaattgaag
     2761 cgaaatgtct tggcttcgcc atcctcccac gttgtcgatc gcaggtcgct tagcgccgcc
     2821 ttaccctcac ccgaatcgag ttcggatacc tgccggaact gcagttgata accctcgcaa
     2881 gcacttccgg aaaaatcata cagaatccgg ccacgcacat cctcgatgcc gcgacttccg
     2941 cgtgacttgg ccagtttgag gtcgtagacc gccctgtgcg gggcgagcaa gacgcgatcg
     3001 acgggcggcg ctgccgatgc cggctggccg gccgccatga cgacgatgcc aaaccctaca
     3061 gcaagccaag ttctcgtgcc gcccgggatt ctcattgtca tgaacctttt ccttcgacca
     3121 aaatggccgg accgcgagca agatgcacga tctgggcaac atagaggctg cggcagattc
     3181 cgccaaatca gtggttttca ggcttgcgta gaagtgcccg attggcgaag gatcgcgcag
     3241 aaacctgtgt cgtggaggac agcatgtctg gttccgttga gaagaaactc gcaaatctgg
     3301 gaatcagcct tcccacgccg gcaactccga ttgccaacta tgtgccgttc gttcgcagcg
     3361 ggaacatgct cgtcatatcg ggccagcttt gctttggcgt ggacggcaat ctcgtggcga
     3421 aaggccagct gggcggcagc gtctcgatgg atgatgggca aaaggctgcg cgcgcctgtg
     3481 ccgtcaactt gctcgcacag ctgaaagccg cactcggcga tctggacaag gtcacgcgtg
     3541 tcattcgtct gggcggcttc atcaattcgg cgcccggctt taccgatgga ccgaaggtca
     3601 tgaacggcgc ctcggacctc atggtcgagg tctttggcga aaaaggccgg cacgcgcgga
     3661 gcactgtcgg cgtcgcagct ttgccggcca acgctgcggt cgaagtggaa ggccagttcg
     3721 aagtctcctg attgcccgat gtcccttgat tggctgactg cgcgacccgt tgcccaccgc
     3781 ggcctccatg atggacagcg cggcatcatc gagaatactg caaccgcctt ctcagcggcg
     3841 ctttcaaata actacggcat cgaaacggat ttgcagttaa gcgccgacgg cgaggctatg
     3901 gtgcatcacg atgatgcgct cggtcgcctg acggagggta gcgggcgtct tgacgcgttg
     3961 agcgcggcac aactcaagag catacgattc aaggcgacgg ccgatcgcat tcttacgctt
     4021 ggcgaactcc tcgatcttgt agccggccga gtcactttgc ttctggaact caagagtcac
     4081 tttaacggcg atgatcggct cctcgcacga accgccaatg tcctttccgc atatcgcggc
     4141 gctgccgcga tcatgtcctt tgatcccgcg ctgaccgagc ttgcccgcca cagatttccc
     4201 gccctggtcc gcggcatcgt cgcacagcgg cactatccac agagcgagtg gaaagtattg
     4261 tcggccgcgg cgagatggaa catgggcctg ctgctgcacg ttccccaaag ccggccccaa
     4321 tttatcgcct attctgtgcg ggatctgccg gctgggccgc cgctgctgat gcgcgcgctg
     4381 tggaaattgc cgttactgac gtggacagtg cgcaacgacg aggatcgaaa gcgtgccgct
     4441 cgctgggcag accaggtcat attcgaaggt tggcggccat aatttgccaa aacatgcccg
     4501 ctagtgatgg ctgtcctgcc ggcgcgcgca agtttggagg tcacaattgg cccgcctgct
     4561 cacacccatc attattattg cggcattttc gggcgtaatc tgcgccgcag ccgccgatcc
     4621 ggaggggctt ttcgacatca ctctctcggc agcgagcaag aaatcaacat ctgccaagcg
     4681 gccgcaaacc ggacagatcg cctgcaccgt gactggctgc cagagaatcc cgcccaattg
     4741 ccgtccggag accggctaca attgggatgg aatccctacc ggtttcgata ttgttgtctg
     4801 tcgatatccc cgcggccggc gcgggtagat gcacgaatcc tggacgaatt catacgtaag
     4861 atgggaatac aataatttca cccgatgact gacttcgaac ttcgcatccg tattacgcag
     4921 tcgcttgccg atgtcccggc gcgggcctgg aatatgtgcg ccgacggcat actggcgccg
     4981 gccgttcaac cgaaatccga gctggaatcc acatctggcg aaaatttgtc ctcgacatta
     5041 tcaacacgaa gaatcccgtc taatccattt gtttcacagg aatttctatc cgctctcgag
     5101 gcatcgggat cggtgggcgg acgaaccggc tggcagccca ggcacctcct cgccgaagac
     5161 accgcaggcg aacttgtggg agtcgcgccc tgctacgtca agatgcactc gcgcggtgaa
     5221 tacgtattcg accatggctg ggcggaagcc ttcgagcgcg cgggcggcaa ctactatcca
     5281 aaacttcagg tcgcggtgcc gttcacgccg gtgaccggcc cgcgcctgct ggtccggcct
     5341 ggactgcaaa ccgatgctgt gcgcggtgct ctcgctgacg ccctggtcga aatcaccacg
     5401 gcgagtgatc tctcctccgc ccatgttacg ttcccgaccg aagacgaatg gcatgcgctt
     5461 ggacagcggc atttcctgcg ccgcactgat cagcagtttc attgggagaa tgccgactac
     5521 aaatcgtttg atgatttcct ggccgcgctt gcatcgcgca aacgcaagac gatccggcgg
     5581 gagcgcaagg aggcgctgac ggccagcatc gaagtgcgtt ggctcaccgg tgccgacctg
     5641 acggaatcgg tctgggttgc tttttttgcc ttatacatgg aaaccggttc ccg
//
LOCUS       DASSRL010000448         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_309645, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000448 DASSRL010000000
VERSION     DASSRL010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309645"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="VFL49_09120"
     CDS             <1..1841
                     /locus_tag="VFL49_09120"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7912181.1"
                     /translation="MLHLARVRTVDKNSNVLEHPAVSLDDIKRFRQLDGKCPGHPEYR
                     LTTGVETTTGPLGQGCGDSVGMAVASRWLGRHFNRPDFPIFDFRVYTLCSDGDMMEGV
                     SSEAASLAGHLKLGNLCWIYDSNRISIEGKTDLAFSEDVAARFRAYGWTVRRVADAND
                     TEQLAHSLELFQHSNIAPTLIVVDSHIGYGAPHKQDTSAAHGEPLGEDEVRLAKRSYG
                     WPEDSRFLVPDGVIEHVHGLIAGRCGPLRHAWSRQITAYRDKYPDMADRLQRMERGEL
                     PDEWAADLPSFPADAKGMASREASGKVLNVLAQHYPWLVAGSADLAPSTKTRLTFDGA
                     GDLEADNPGGRNLHFGVREHGMGTILNGLALSKLRACGSTFLIFSDYMKPAIRLGALM
                     RLPVIYVFTHDSIALGQDGPTHQPVEQLVALRAIPNLITLRPADANEVTEAWRVICTL
                     KDRPACLILSRQALPTFDRSKYAPADGVKRGAYVLADVPGRTPDVILIGTGSEVALCA
                     EAHEALKKDRVASRVVSMPSWELFEQQDKPYRDHVLPPGITERVSVELGSVIGWERYV
                     GPRGTSIGMHQFGASAPAKDLLRKFGFTCEAVVAAAKRQVKDIVRE"
     gene            1844..>2999
                     /gene="tal"
                     /locus_tag="VFL49_09125"
     CDS             1844..>2999
                     /gene="tal"
                     /locus_tag="VFL49_09125"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7912182.1"
                     /translation="MTPARKTDKNPLKALHSQGQTVWLDFLSRRFLADGGLKKLIAND
                     GLTGVTSNPSIFEKAIAESNDYDASLREAEDRADLDVMALYEHLAIADIRSAADDLKP
                     VFDRTHGADGYASIEVSPYFAADTEATVSEARRLARLVDRPNVMVKVPATKAGLPAIR
                     RLTADGVNVNITLLFSQDVYEEVVDAYLTGLEQFVAQGGDPSRVASVASFFVSRIDVA
                     VDKLVEEQLRKTANADKRRSLSGLKGTVAIANAKLAYQRYKRLFSGARWQKLQARGAR
                     SQRVLWASTSTKTPGLSDVLYVDGLIGRDTINTMPPKTVDAFRDHGKVRPSLEEDVEG
                     AGRTMRELARLGISIDAVTAQLTVDGVRQFSDAFDKLLGSLARKRAALLGD"
BASE COUNT          636 a          905 c          885 g          573 t
ORIGIN      
        1 caatgctgca tctcgcccgc gtgagaacgg tcgataaaaa cagcaatgtt ctcgagcacc
       61 cggccgtaag ccttgacgac atcaaacgat ttagacagct cgacggaaaa tgccctggac
      121 accccgaata tcggctcacc acgggagtcg aaacgacaac cggcccactg gggcaaggct
      181 gcggcgacag cgtcggcatg gctgtagcct cccgctggct cggccgccat ttcaatcggc
      241 ctgattttcc gatcttcgat ttcagggtct acacgctctg cagtgacggc gacatgatgg
      301 aaggtgtctc cagcgaggca gcatcactcg ccgggcatct gaaactcgga aacctctgct
      361 ggatctacga cagcaaccgt atcagcatcg agggcaagac ggatctcgct ttcagcgaag
      421 acgtagccgc gcggtttcgg gcctacggct ggaccgtccg tcgggttgct gacgccaatg
      481 ataccgaaca actggcgcac tctcttgaac tgttccagca ttccaacatt gccccgacgc
      541 tgatcgtcgt cgacagccac atcggttacg gcgcaccgca taagcaggat acgagcgccg
      601 cacatggcga acctctcggc gaagatgaag ttcgtctcgc caaacgaagt tatggctggc
      661 ctgaagattc caggtttctc gttcccgacg gcgtaatcga gcacgtccac ggcctcattg
      721 ccgggcgctg cggcccgctc cgccacgcat ggtccaggca gattaccgca taccgcgaca
      781 aataccctga tatggccgac cggcttcagc gcatggagcg cggagaactt cctgacgaat
      841 gggccgccga ccttccgagt tttcctgcag atgccaaagg gatggccagt cgtgaggcgt
      901 ccggcaaggt gcttaacgtg cttgcccaac actatccctg gcttgttgcc ggatcagccg
      961 acctggcgcc ctcgacaaaa acgcgactga catttgacgg cgcgggcgac ctcgaagcag
     1021 acaacccggg cggtcgcaat cttcattttg gtgtgcgtga gcatggcatg ggcacgatcc
     1081 tgaacggcct tgcgttgtcg aaacttcgcg cttgcggctc aacctttctc atcttttccg
     1141 actatatgaa gccggccatc cgcctcggcg cgctgatgcg ccttccggtg atctacgtat
     1201 tcacgcacga ctcgatcgcg ctcggacaag acggccctac ccaccagccg gtggagcaac
     1261 tcgtcgcact gagagcaatt cccaatctca tcacgctgcg cccggccgac gccaatgagg
     1321 tgaccgaggc ctggcgcgtt atctgcaccc tgaaagaccg gccggcatgt ctgatcctca
     1381 gccggcaggc attgccaact tttgaccgtt cgaaatacgc gccggcagat ggcgtaaagc
     1441 gcggcgccta cgtgctcgcc gatgttcccg gcaggacacc ggacgtcatc ctcatcggta
     1501 cgggaagcga ggtcgcgttg tgtgccgagg cgcatgaagc acttaaaaaa gaccgcgtcg
     1561 catcacgggt tgtgagcatg ccgtcctggg aattgttcga gcagcaggac aagccttatc
     1621 gcgatcacgt gttgcctccc ggcattaccg agcgcgtatc ggtcgaattg ggctcggtga
     1681 tcggctggga acgctacgtc ggcccgcgcg gtaccagtat cgggatgcat caattcggtg
     1741 cgtcggcacc ggcaaaagat ctcctgcgca aattcggctt tacctgcgaa gcggtcgtcg
     1801 ctgctgccaa gcgccaggta aaagatattg tgagagagta gtgatgaccc ctgcccgcaa
     1861 gacagacaaa aatccgctca aggcattgca cagccagggc cagacggtat ggctggattt
     1921 tctgtctcga cgtttccttg ccgacggcgg acttaaaaaa cttatcgcaa atgacggact
     1981 gactggcgtc acatcaaacc cgtcgatttt cgagaaagcg attgcggaaa gcaacgacta
     2041 cgacgcgtcc ttgcgtgaag cggaagaccg cgccgatctg gacgtgatgg cgctgtatga
     2101 gcacctcgcg atcgctgata ttcgcagcgc ggctgacgac ctcaagccgg ttttcgaccg
     2161 gacgcacggc gccgacggat atgccagtat tgaggtatcg ccatattttg cggcggacac
     2221 agaagcgacg gtgtcggaag cgcggcgcct tgccaggctt gtagaccggc caaacgtcat
     2281 ggtgaaagtg cccgcgacga aggccgggct tccggccatt cggcggctga cggccgatgg
     2341 cgtcaatgtg aatattacct tgttgttttc gcaggacgtc tatgaagagg tcgtcgacgc
     2401 ctatctgacc ggccttgagc agttcgtcgc gcaaggaggc gatccaagcc gagtcgcgag
     2461 cgttgcaagc tttttcgtca gccgtatcga cgtggcggtg gacaagctcg ttgaagagca
     2521 gcttcgaaaa accgccaacg cggacaaacg ccgaagcctg tccggtctca agggaacagt
     2581 cgccattgcc aacgccaagc tcgcctatca gcgttacaag cgcctttttt ccggcgcgcg
     2641 ttggcaaaaa ttgcaggccc gcggtgcccg cagccagcgc gtattgtggg cgagcacaag
     2701 tacaaagacc cccggactca gcgacgtgct ctacgtcgac gggctgatcg gccgcgacac
     2761 gatcaatacc atgccgccaa aaacggtgga tgcattccgg gaccacggaa aggtgcggcc
     2821 gagcctcgaa gaggatgtcg aaggtgccgg gcgcaccatg cgcgaacttg cgcgtctggg
     2881 catttcgatt gacgcggtga ccgcgcaact gactgtcgat ggtgtgcggc aattttccga
     2941 cgcattcgac aaactgcttg gatcgcttgc gcgcaagcgc gcggccctgc tcggagatg
//
LOCUS       DASSRL010000449         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_344475, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000449 DASSRL010000000
VERSION     DASSRL010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344475"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFL49_09130"
     CDS             <1..433
                     /locus_tag="VFL49_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HET7912183.1"
                     /translation="FLEYWGNPEATRDKFIGDWMTTGDQGVMDEEGYVSFVGRDDDVI
                     TSAGFRIGPGEIEDCLIRHPAVALAAAIGKPDALRTEIVKAFIVLKSGYDASDSLASE
                     IQGFVKTRLSAHEYPREIAFIDDMPMTTTGKVIRRLLRQRA"
     gene            complement(436..1827)
                     /locus_tag="VFL49_09135"
     CDS             complement(436..1827)
                     /locus_tag="VFL49_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET7912184.1"
                     /translation="MALPSFAPENAKAAPLPALARILARVKSVLAERNDSRLAQFVAG
                     KVFLVRVGSALLALVSQVLLARWMGSFEFGIYIYVWTWVLMIGALTDVGLSSAARRFI
                     PEFTELRNFDRLRGFISGSRWLGFGMATAVGLVGALGVRFLAPWLDHFAVIPLYLACL
                     AIGAYGLVQVQAGIAQSYDWPNLALMPFYIWRQLIITVLMGTAWFFGAPTGAVTAMII
                     AVVTTWAVTIGQLVLLNRRLRGKVPEGRKVYEPKIWFATSLPIFVIEGFYLLLTYVDI
                     IALEHFGSPHDVAIYYAGARLLAIVAFVYFAIAGATTHRFSEYDVSGDRQRLASFFRE
                     TVHWTFWPSLAVCAAILAFGRPLLSLFGAGFEAGYSVMFILAIGLLARASVGPAERLL
                     NMIGQRRQCATVYAVAFVVNLTLCVFLIPRHGIEGAAAATSTALVAESLMFSWVAKQR
                     LGFTIFVFGRPKT"
     gene            complement(1889..>2489)
                     /locus_tag="VFL49_09140"
     CDS             complement(1889..>2489)
                     /locus_tag="VFL49_09140"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7912185.1"
                     /translation="LGADGILAILEAYFPLKDAQIESYFRAIADAVDIPIVLYTNPQF
                     QRSDLSLDVVARLSEHPRIRYIKDASTNTGRLLSIMNRAPRMKVFSASAHIPTAVMLI
                     GGVGWMAGPACIVPRQSVRLYELCRAQQWPEAMKLQRDLWRINEAFARFNLAACIKAG
                     LQIQGYAVGDPLPPQAALGPAEREAVEAILADVAKLEMP"
BASE COUNT          552 a          757 c          778 g          402 t
ORIGIN      
        1 gtttctcgaa tactggggca accccgaggc cacgcgcgac aagttcatcg gtgactggat
       61 gacgaccggc gaccagggcg tcatggatga ggagggttac gtcagcttcg tcggccgcga
      121 tgacgacgtc atcacgtcgg cgggcttccg gatcggaccc ggcgaaatcg aggattgcct
      181 gatccgccat ccggcagtgg cgctcgcggc tgcgatcggc aagccggatg cattgcgcac
      241 cgaaatcgtc aaggccttca tcgtgctgaa aagtgggtac gacgcatcgg attcactggc
      301 gtcggagatt caaggtttcg tgaaaacgcg tctgtccgca cacgaatatc cgcgcgagat
      361 cgcctttatc gacgacatgc ccatgacgac cacgggcaaa gtcatccgcc gcctgctgcg
      421 gcagcgagcc tgaggttacg tcttcggccg gccgaaaacg aagatagtga atccgagccg
      481 ctgcttcgcc acccacgaga acatcaggga ttcagcgacc agcgcggtgg aggttgcggc
      541 cgccgcgccc tcgattccgt ggcgcggaat aaggaaaacg catagcgtca ggttgacgac
      601 gaacgcgacg gcgtagacgg tcgcgcactg tctgcgctga ccgatcatat tgagcagtcg
      661 ttcggccggc ccgacggagg cgcgcgccag caatccgatc gccagaatga acatcacgct
      721 gtaacccgct tcgaaccccg caccgaacag cgacagaagc ggcctgccga acgcgaggat
      781 cgccgcacag actgccagcg agggccagaa cgtccagtgc accgtctcgc ggaaaaatga
      841 cgcgagccgc tgccggtcgc ctgagacatc gtattcgcta aatctgtgcg tcgttgctcc
      901 cgcgatggcg aaataaacaa atgccacgat tgcgagcagg cgggcacccg cgtagtagat
      961 tgcgacgtca tgcggcgagc cgaaatgttc aagcgcgata atgtccacgt aagtgagcaa
     1021 caggtagaag ccttcgatga cgaatatcgg gagcgacgtc gcgaaccaga ttttcggctc
     1081 atagactttc cgtccctccg gcactttgcc gcgaagtcgc cggttaagca gcacaagctg
     1141 accaatcgtc accgcccagg tcgtgacgac ggcgatgatc atggccgtca cggcgccggt
     1201 cggcgcgcca aagaaccagg cggtacccat cagcaccgta atgatgagct ggcgccagat
     1261 gtagaagggc ataagcgcca ggttcggcca gtcgtaggac tgcgcgatgc cggcctgcac
     1321 ctgcaccagg ccataggcac cgatcgcgag gcaggcgaga tagagtggaa tgacagcaaa
     1381 gtggtcgagc cagggcgcga gaaagcgcac accgagagcc ccgacaagac caacggccgt
     1441 cgccatgccg aagcccagcc agcgcgagcc gctgataaag ccgcgcagcc ggtcaaagtt
     1501 cctgagctcg gtgaattcag gaatgaagcg gcgcgccgcg gacgacaggc cgacatcggt
     1561 gagtgcaccg atcatcagca cccacgtcca cacataaatg tagatgccga actcgaagct
     1621 tcccatccag cgcgccagca gaacctgcga caccagcgcg agcaatgcgc tgccgacacg
     1681 caccagaaaa acttttccgg cgacgaattg cgccagccgg ctgtcgttgc gttcggcgag
     1741 aacggatttg acgcgcgcaa ggatgcgcgc caatgccggc agcggcgcag cttttgcatt
     1801 ttcgggtgcg aaggacggca aggccatcgc cttcgcctag caaggaaccg ttaacattca
     1861 gttggatggc attcacaaag ccgccagatc agggcatttc cagctttgcg acgtcggcga
     1921 ggattgcttc aaccgcttcg cgctcggccg gtccgagcgc cgcttgcggc ggcagcggat
     1981 cgccgacggc atagccctgg atctgcaatc cggccttgat gcaggcggcg aggttgaagc
     2041 gcgcgaaagc ctcgttgatg cgccagagat cgcgctgcag tttcatcgct tcgggccatt
     2101 gctgcgcgcg gcacagctca tagagacgca cgctctggcg cggcacgatg caggccgggc
     2161 ccgccatcca gccgacaccg ccgatcagca tcacagccgt tggaatgtgc gccgacgcag
     2221 aaaacacttt catgcgcggc gcgcgattca tgatcgacaa caggcgcccg gtattggtcg
     2281 aagcatcctt gatgtagcgg atgcgcggat gctcggacag ccgtgcgacc acgtcgagcg
     2341 aaaggtcgct gcgctggaat tgcgggttcg tgtagagcac gatgggtatg tcaacggcgt
     2401 cggcaatcgc gcgaaaataa gattcgatct gcgcatcctt cagcggaaag taggcctcga
     2461 gaattgccag aatgccgtcg gcaccgagt
//
LOCUS       DASSRL010000450         4429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_414688, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000450 DASSRL010000000
VERSION     DASSRL010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414688"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="VFL49_09145"
     CDS             <1..1769
                     /locus_tag="VFL49_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7912186.1"
                     /translation="TEHLVANNIPYVFGICGHGNVGMLDPLYQARDRITLISPRHEQV
                     AAHMADAYFRVRHKPVATLTSCGPGSCNIIMPLACALTDSSAMLAITANVPTSQFNRS
                     PFQEINKHFQADFVNIVRPVVKRSYQPTRVDMLPLALRQATTTMLSGRPGPVNIDVPF
                     NVFQEDDDVELPPPTPPLNAQRSGASPDEVSRALDMIFKAQRPVIFIGHGVTLSEASR
                     ELTEFARKLGIPVISSPNGMGCFDMTDPLSLGFIGRNGAYPANEAGRRADLVVAIGAR
                     FDDRSASSWMPGYSWNFPQTKLVHVDVDPAELGRNYPPDIGVLADARTFLRQLLAELE
                     RRGSKGGESTKAWLADIAKWRADWDKFTAPNFDLHASPIRPERVVADCQAVLPPDAIL
                     CCDVGVSHNWYMQFWKARRPQTMLNSWGFSGMGFGAAGALGAKLAAPDRPVVAVTGDG
                     CFTMVPHVLCTAVEYNIPVVWVIWNNFGWASIRDIQLGMFGGREHGTMFYQGDNRKPY
                     NPDFAAWAKATGVEALTISRSQDFKGALEHAIKANKPFVLDVHVDAEVRPPSTGAWEL
                     PPTPYKEPVFGQRWIPDAAPVK"
     gene            1947..2729
                     /locus_tag="VFL49_09150"
     CDS             1947..2729
                     /locus_tag="VFL49_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7912187.1"
                     /translation="MEPSRGGIQSIGRAFAILEEVARHRDGIGLADLSKRVGLHNSTT
                     FHLAKTMVALGYLRQIKESKRYRIGRPLFALAASALDEKEMVSMATPVLDELSRRTGE
                     SSHYASRMSDAVVVMARTPGPGAFQLTDRVGVVRPAYCTALGKIILAALLPDQLDRYL
                     ERVELKQLTPKTITNPQRLRRELQDIRKTGIAFDDGEFDNEVRCAAMPVHDFSGQVIG
                     AIGISAPVWRLSIQALQGKARNISEAARKLSEAFGASQSSDK"
     gene            2734..3024
                     /locus_tag="VFL49_09155"
     CDS             2734..3024
                     /locus_tag="VFL49_09155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET7912188.1"
                     /translation="MTYVIVAYWRPRDGQGEKIEAILRELASAVRQEPGNLEFIVNRS
                     HDDPNEFLLYEQYKDEQAFIDHQQTPHFRKLVLEGAVPLLERRERHAYSIVA"
     gene            3054..3758
                     /locus_tag="VFL49_09160"
     CDS             3054..3758
                     /locus_tag="VFL49_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7912189.1"
                     /translation="MSLAGKKLVVVGGSSGIGLATAELARNEGAEVVVASRNAERLKA
                     AADKIGATGIAADVTSDESVSGLFRKCGPVDHVVVTAAQLRTGPFKTVSMDDVRATME
                     SKFWGAWRVARSAEIRGNGSLTLVTGYLSVRPRPGAAIVGAANGALESLTRSLALELA
                     PVRVNAVSPGTIDTPIRASMPEAARRDMLAKTAAALPVGRVGAGEDIARQILTFMTVG
                     FATGSVVYIDGGALVV"
     gene            3794..>4429
                     /locus_tag="VFL49_09165"
     CDS             3794..>4429
                     /locus_tag="VFL49_09165"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7912190.1"
                     /translation="MAQRTAYVPHGVIPAVLMPFDNDLAIDEASFRRHLRDVASVEGL
                     SAITINAHSTEVASCTFDEQKRVLDIAQDEIGARLPLVNGIWADGSIEAARIARMATQ
                     GGASALLVFPPAPFTLGQSPAMALAHVRRIADATDLPLIVFQYPLATGQGYPKETLLK
                     LVDEVPSVRAIKDWIGNVPHHEWHIRTLQNLPRPVNVLTTHSSWLFSSLVLG"
BASE COUNT          808 a         1415 c         1385 g          821 t
ORIGIN      
        1 taaccgagca cctggtcgcc aacaatattc cctatgtgtt cggcatctgc ggccacggca
       61 atgtcggcat gctcgacccg ctctatcagg cgcgcgaccg catcacgctg atttcgccgc
      121 gccacgagca ggtcgcggcc cacatggccg acgcctattt ccgcgtgagg cacaagccgg
      181 ttgccacgct gacctcttgt ggaccgggtt cctgcaacat catcatgccg cttgcctgcg
      241 cgctgaccga ctcctcggcc atgctggcga tcaccgccaa cgtgccgacg tcgcagttca
      301 accgcagccc gttccaggag atcaacaagc attttcaggc cgatttcgtg aacatcgtgc
      361 ggccggtcgt gaagcgcagc tatcagccga cccgtgtcga tatgctgccg ctcgccttgc
      421 ggcaggcgac cacgaccatg ctctcaggcc ggccggggcc agtgaatatc gacgtgccgt
      481 tcaacgtctt tcaggaagat gacgacgtcg aattgcctcc gccgactccg ccgctcaacg
      541 cgcagcgcag cggggcctct cctgatgagg tctcccgcgc actcgacatg atcttcaagg
      601 cgcaacgtcc ggtcatcttc atcggccatg gcgtcacgct ctcggaagca agccgcgaac
      661 tcaccgaatt cgcgcgcaag ctcggcattc cggtcatctc ctccccgaac ggcatgggct
      721 gtttcgacat gacggatccg ctgtcgctcg ggttcatcgg ccgcaacggc gcttatccgg
      781 ccaacgaagc gggacgccgc gccgatctgg ttgtcgcgat cggcgcgcgc ttcgacgatc
      841 gctcggcgtc gtcgtggatg cccggctatt cgtggaattt tccgcagacc aaacttgttc
      901 atgtcgacgt cgatcccgcg gagctcggcc gcaattatcc tcccgatatt ggcgttctgg
      961 ccgatgcacg cacattcctg cgccagcttc tcgccgagct cgagcggcgc gggagcaagg
     1021 gcggggaaag caccaaggcg tggctcgccg acatcgcgaa atggcgcgct gactgggaca
     1081 aattcaccgc gccgaatttc gaccttcatg cctctccaat ccgtcccgag cgcgttgttg
     1141 cggattgcca ggctgtgctg cccccggatg caatcctttg ctgcgacgtt ggcgtcagtc
     1201 acaattggta catgcagttc tggaaagcgc ggcgcccgca gacgatgcta aactcatggg
     1261 gcttttccgg tatgggcttc ggcgcagccg gcgcgcttgg cgccaagctt gccgcgcccg
     1321 accgtcctgt cgtcgccgtc accggcgacg gctgcttcac gatggtgccg catgtgctct
     1381 gcaccgcggt cgaatacaac atcccggtcg tctgggtgat ctggaataat ttcggctggg
     1441 cctccatccg cgacattcag ctcggcatgt ttggcggccg cgagcacggc accatgtttt
     1501 atcagggcga taatcggaag ccgtataatc cggatttcgc ggcctgggcg aaagccaccg
     1561 gcgtcgaagc gctgacgatt tccagatcgc aggatttcaa gggcgccctt gaacacgcca
     1621 taaaggcgaa caagccgttt gtgctcgacg tccacgtcga tgccgaggtg cggccgccgt
     1681 cgaccggcgc atgggaattg ccgccgaccc cttacaagga gccggtgttc ggacagcgtt
     1741 ggattcccga tgccgctccg gtgaaataaa gttggatatg tcataaagtt cgcggggcgc
     1801 gcgcgcgaac gaaaccgttt gggagctttg ttttgagaag cagaatccgc tcgaaagcca
     1861 gcctggcccc gcgtcggcct gctcgcactg cggcaacggg cgcgcgacct gttgaagcga
     1921 cgcgccggcc tgttgacgaa gatgccgtgg agccgtcgcg cggcgggatc caatcgatcg
     1981 gtcgcgcctt cgctattctg gaggaagtgg cgcgtcatcg cgacgggatc gggctggctg
     2041 atctcagcaa gcgggtcggc ctgcataaca gcacgacctt ccatctggcg aagacgatgg
     2101 tcgcgctcgg ctatctccgg cagatcaagg aaagcaagcg ataccggatc gggcgtccgc
     2161 tctttgccct tgccgccagt gcgctcgatg aaaaggaaat ggtgagcatg gcaacgcccg
     2221 tgctcgacga actgtcgcgc cggaccggcg aaagcagcca ctatgccagc cgcatgagcg
     2281 acgctgtcgt cgtgatggcg cgtacccccg gaccgggggc gtttcaactc accgaccgcg
     2341 tcggcgtggt gcggccggcc tactgcaccg cgctcggcaa gatcatactc gccgccttgc
     2401 tgccggatca gcttgatcgt tacctcgagc gggtggagct gaagcagctg actccgaaaa
     2461 caatcaccaa tccgcaacgc ttgcggcgcg agcttcagga cattcgcaag accggaattg
     2521 ccttcgacga cggcgaattc gataatgaag tgagatgcgc ggcgatgccg gtgcacgatt
     2581 tcagcggcca ggtcatcgga gcgatcggaa tttccgcgcc ggtctggcgg ctgtcgattc
     2641 aggccttgca gggcaaagca cgcaatatca gcgaagccgc caggaagctg tcggaggctt
     2701 tcggggcgtc ccaatcatcc gataaatgac ccaatgacat acgtgatcgt ggcctattgg
     2761 cgcccacgcg acgggcaggg cgaaaaaatc gaggcgatct tgcgcgagct tgcctcggca
     2821 gtccggcagg agccgggcaa cctggagttc atcgtcaatc gctcgcacga cgatccgaat
     2881 gaatttctct tgtacgaaca gtacaaagac gaacaggcgt tcattgacca ccagcagacg
     2941 ccacacttca ggaaactggt gctggagggc gcggtgccgc tgctcgaacg gcgggaacgc
     3001 cacgcctatt ccattgtcgc ttagtcgctt ctgcaaaaaa gaaagcccca cgcatgtcgc
     3061 ttgccggaaa gaaacttgtt gtcgtcggag gctcatccgg cattggtctt gccactgccg
     3121 agcttgccag gaacgagggc gccgaggtcg tggtcgcttc gcgcaatgcc gagcgcctga
     3181 aggcggctgc cgacaagatc ggcgcgacag gcattgcggc cgatgtcacc agcgatgaaa
     3241 gcgtttctgg ccttttcagg aaatgcgggc cggtcgacca tgtcgtcgtg accgccgctc
     3301 agttgcgcac cgggcctttc aagacggtat ccatggacga cgtgcgcgcg accatggaaa
     3361 gcaagttctg gggcgcctgg cgtgttgcgc gttcggctga aatccgcggc aacggctcgc
     3421 tcacgctggt caccggctac ttgagcgtgc gcccgcggcc cggcgccgcg atcgtcggcg
     3481 ctgccaacgg cgcgcttgaa tctctgacca ggagtctggc gctggaactg gccccggtca
     3541 gggtaaacgc cgtgtcgccc ggaacgatcg acacgcccat ccgcgcaagc atgccggagg
     3601 ctgcccgccg cgacatgctt gcaaagaccg cggctgcttt gcctgtcggg cgcgtcggcg
     3661 cgggcgaaga catcgcccgc cagatcctga cgttcatgac ggtcgggttt gcgaccggat
     3721 cggttgtcta tatcgatggc ggggcgttgg tcgtctgact atactggcgg cggcgatctc
     3781 gacggagaag cgcatggcac agcggaccgc ttatgtccct cacggcgtga ttccggcggt
     3841 tctgatgccg ttcgacaatg accttgcgat cgacgaggca agcttccgca ggcatctgcg
     3901 cgacgtggcg tcggtcgaag gcctgtcggc gatcaccatc aacgcccact cgaccgaagt
     3961 cgcctcctgc accttcgacg agcaaaagcg cgtgctcgat atcgcgcagg acgagatcgg
     4021 cgcgcgcctg ccgctggtca acggcatctg ggccgacggc agcatcgaag cggcgcgcat
     4081 cgcgcgcatg gcaacgcaag gcggcgcgtc ggcgctgctg gtgtttccgc cggcgccgtt
     4141 cacgctcggc cagtcgccgg ccatggcgct ggcgcacgtc aggcgcattg cggacgcgac
     4201 cgatctgccg ctgatcgttt tccagtatcc gctcgcgacc gggcagggct atccgaagga
     4261 aacgctgctc aagctcgtcg atgaagtgcc gagcgtgcgc gcgatcaagg attggatcgg
     4321 caacgtgccg catcacgaat ggcacatccg cacgctgcaa aacctgccgc gcccggtgaa
     4381 cgtgctcacc acgcatagct cctggctctt ctcctcgctg gtgctcggg
//
LOCUS       DASSRL010000451         6505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1534464, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000451 DASSRL010000000
VERSION     DASSRL010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534464"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFL49_09170"
     CDS             <1..275
                     /locus_tag="VFL49_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HET7912191.1"
                     /translation="EDLHAILLKFYMKEPFVHVLPFGETPQTRHVRGSNMTFIGVAKD
                     RVPGRAIVISALDNLVKGASGQAIQNMNLVMGWPETMGLEQVALFP"
     gene            322..1935
                     /locus_tag="VFL49_09175"
     CDS             322..1935
                     /locus_tag="VFL49_09175"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HET7912192.1"
                     /translation="MFEVQNQPPPLEPYNLFASDAVLREAVRREGASWAVGELTELGA
                     RLGKPETVQLGFDANRFPPQLRTLDRYGHRLDEVEFHPAWHGLLEIALAAGLHSSPWA
                     NPKPGAHVARAAGTYMLGQIESGVYCPLAMTYGSVPTLRHAPQIADEWLPRIYSRQYD
                     RRFRPARLKTSALLGMGMTENQGGSDLRTNTTRAQPSADGSFRLYGHKWFMSAPMCDA
                     FLVLAQAPNGLSCFLMPRWTPDGERNAILILRLKDKLGNRSNASSEVEFHGAYAQLIG
                     EEGRGIPTIIEMGNYTRLDCCIGSSALMRQAVAQAIHHARHRIAFQKKLVDQPLMTNV
                     LADLALESEAAIVLTMWLARAYDDNNESAQVIRRVITPAAKFWICKRTPFLTYEAMEV
                     LGGSGYIEESVMPRLYREAPVNSIWEGSGNIMCLDVLRAMERTPNAAGILHRELGEAN
                     DARVKAFAERIEKRIAARDHADESQARALVRELIVALQASLLVRHAPSSVADAFCASR
                     LGGEGSSVFGLLPRGLDMRGIAERAGPAS"
     gene            complement(1932..2330)
                     /locus_tag="VFL49_09180"
     CDS             complement(1932..2330)
                     /locus_tag="VFL49_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET7912193.1"
                     /translation="MTKELMWLTLTIILTGLMWVPYIIDRLMVRGLMGSMANPSRHDK
                     PQSAWAQRLYFAHTNAVENLIIFAPLVLILDAQGYSTESTVIACAVYFWARLAHVIVY
                     TMGVPVLRTLAFAVGFVAQVALVFAVFGKL"
     gene            2463..2720
                     /locus_tag="VFL49_09185"
     CDS             2463..2720
                     /locus_tag="VFL49_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912194.1"
                     /translation="MAASAKPEIKAFFDEPTNMVSYLVSDPATREGAVIDPVLDFDRR
                     SGDATVKSADEILADAKRLGVAITRILETRAHADHLSARTI"
     gene            complement(2754..4565)
                     /gene="phaC"
                     /locus_tag="VFL49_09190"
     CDS             complement(2754..4565)
                     /gene="phaC"
                     /locus_tag="VFL49_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HET7912195.1"
                     /translation="MDRSTTEPAKVGDVDIEALSRNLARLIEEGGKALAAYLKPREEG
                     KIRNDHAEDLTDAVKTVGRVVEYWMSDPRRAIELQTSLGRAYLELWAGAVKRMAGEQA
                     EPVVSPDPRDKRFSDPEWSQNQFFDFLKQAYLLTAHWADKLVKDAAGVDEHTRKKAEF
                     YVRQIANAISPSNFVLTNPELLRETLSSNAGNLVRGMHMLSEDIKAGQGVLKIRQSDP
                     SMFDVGKNLATTPGKVVFQNELMQLIQYEGTTKNVLKLPLLIVPPWINKFYILDLAPE
                     KSFIKWCVDQGITVFVISWVNPDARLAAKSFEEYMREGVITALDTIEAITGEKKTHTI
                     GYCVGGTLLSITLAYLAAKKQNRVKSATLFAAQIDFTYAGDLMVFVDEERIRRLETHM
                     KEQGYLEASRMATTFNMLRSNDLIWPYIVNNYMRGKKPLPFDILYWNSDATRMPAANH
                     SFYLRNCYLDNKLTKGEMEIAGTRLDLHTVKVPVYNLATREDHIAPAKSALYGSQFFG
                     GPVKYVLAGSGHIAGVINPPGKPKYQYWTGGKPNGDSVEAWMESATEHPGSWWPDWLA
                     WLKAQGTETVAARKIGRGKLKPIEDAPGSYVKVKSWT"
     gene            4777..5127
                     /locus_tag="VFL49_09195"
     CDS             4777..5127
                     /locus_tag="VFL49_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912196.1"
                     /translation="MSVKYFLWAGLSFATVLTGCAPGPVIDRLPGDLGLPAAAPARPE
                     TPYEYPAVHDMPPDRASSPMTEEEQVRLEKELIKVRDRQEGQPPATKKGAPAAKKPPK
                     DTQNAQTDGAKGKP"
     gene            5191..6408
                     /locus_tag="VFL49_09200"
     CDS             5191..6408
                     /locus_tag="VFL49_09200"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET7912197.1"
                     /translation="MEEFYRIRRLPPYVFEEVNKAKAKARNAGADIIDLGMGNPDLPA
                     PAHVIEKLKDTLGKPRTDRYSASRGIPGLRRAQAAYYDRRFGVKLNPDTQIVTTLGSK
                     EGFANVAQAITAPGDVVLVPNPSYPIHAFGFLMAGGAIRSVPSEPTPNFFHTLERAIV
                     HSIPKPTAVVVCYPANPTAYVATLDFYKDLVAFAKKHGILVLSDLAYAEVYFDNNPPP
                     SLLQVPGANDIAVEFTSMSKTFSMPGWRIGFAVGNERVIAALSRVKSYLDYGAFTPVQ
                     VAATAALNGPDDCVKEMRATYRRRRDVLVESFGRAGWHVPAPRASMFAWAAIPEPFNT
                     LGSVEFSKLLVEKADIAVSPGIGFGEYGEGYVRIALVENEQRIRQAARNVRRFLDHAP
                     EQLHNVVPLATRR"
BASE COUNT         1265 a         1932 c         1967 g         1341 t
ORIGIN      
        1 ccgaggatct tcatgcgata cttttaaagt tctacatgaa ggaaccgttc gtgcacgtcc
       61 tgcccttcgg ggaaacaccg cagacgcggc atgtgcgcgg gtccaacatg acattcattg
      121 gtgtcgcaaa agaccgtgtc ccggggcgcg caatcgtcat ctccgcgctc gacaatctgg
      181 tcaagggtgc gtccgggcag gcaattcaga atatgaatct ggtcatgggg tggccggaga
      241 cgatgggatt ggaacaggtc gccctgtttc cctgattcca ccggtataat cgccgcgtct
      301 tttgccggag acttacggcc gatgttcgaa gttcagaacc agccgccgcc gctcgagcct
      361 tacaatctgt tcgcgagcga cgccgtattg cgcgaggcgg ttcgccgtga gggcgcaagc
      421 tgggcggtcg gcgaactgac cgaactcggc gccaggctcg gcaagcccga gacggtgcag
      481 cttggtttcg atgcaaacag atttcccccg caacttcgca ctctcgatcg ctacggtcac
      541 cggctcgatg aagtcgaatt tcatcccgcc tggcacgggt tgctcgaaat tgcgcttgcg
      601 gccggacttc actcgagccc ctgggcgaat ccaaaaccgg gtgcccacgt cgcgcgtgct
      661 gcgggcactt acatgctcgg acagatcgaa agcggcgtgt actgcccgct ggcgatgact
      721 tatggctcgg tcccgacgct gcggcatgct ccgcaaatcg cggacgaatg gctgccgcgc
      781 atttattcgc gccagtacga ccgccgcttc cggccggcgc gcctgaaaac ctccgcgctg
      841 ctcggcatgg gcatgaccga gaaccagggc ggctcggacc tgcgcaccaa tacgacacgc
      901 gcgcagccat ccgccgacgg cagttttcgt ctctacggcc acaaatggtt catgtcggcg
      961 ccgatgtgtg acgcgttcct tgtgctggcc caggctccga acggcttgag ctgttttttg
     1021 atgccgcgat ggacgccgga cggcgagcgc aacgccatcc tcatccttcg gctcaaggac
     1081 aaactcggca accgttcgaa cgcgtcgagt gaggtcgagt ttcatggcgc ttatgcccag
     1141 ctcatcggcg aagagggacg cggaattccg actattatcg agatgggcaa ctacacacgg
     1201 ctcgattgct gcattggttc ctctgcactg atgcggcagg ctgtcgccca ggcaattcac
     1261 cacgcgcgac atcgcatcgc attccagaaa aagcttgtcg accagccgct gatgacaaac
     1321 gtgctggccg atcttgcgct cgagtcggag gcggcgatcg tgctgaccat gtggctcgca
     1381 cgcgcgtatg acgacaacaa tgaatcagca caagtgatcc ggcgtgtgat cactcccgcg
     1441 gccaagttct ggatctgcaa gcggacgcca ttcctgacct acgaagccat ggaggtgctc
     1501 ggcggctccg gttacatcga ggagagcgtc atgccgcggc tctatcgcga ggcgccggtc
     1561 aactcgatct gggaaggctc cggcaacatc atgtgtctcg atgtgctgcg cgcgatggag
     1621 cgaacgccca atgcggccgg gattttgcac cgcgagctcg gcgaggccaa cgatgcacgc
     1681 gttaaggcat tcgccgaacg cattgagaag cggattgccg ctcgcgacca cgccgacgag
     1741 tcacaggcgc gcgcgctggt gcgggagctt atcgttgcac ttcaggcttc gctgctggtc
     1801 aggcacgcac catccagcgt cgcggatgca ttttgtgcat cgcgacttgg cggcgagggg
     1861 agttcggttt tcgggctgct gccgcgcggc ctcgatatgc gcggcattgc cgaacgtgcc
     1921 ggtcccgcaa gctaaagctt tccgaacacc gcaaatacga gcgcaacctg tgcgacgaac
     1981 ccgacggcaa aggcaagagt gcggagaacc ggcacgccca tggtgtaaac gatgacatgt
     2041 gccagcctcg cccagaaata gaccgcgcag gcaatgacgg tggactcggt cgaatagccc
     2101 tgcgcgtcga gaatgagcac cagcggcgcg aaaatgataa gattctcaac cgcgttggtg
     2161 tgtgcgaaat agagccgctg tgcccaggct gattgtggct tgtcgtgccg cgagggattg
     2221 gccatggatc ccatgagacc gcgaaccatc agacgatcga tgatgtacgg cacccacatc
     2281 aggccggtca ggatgatcgt cagcgtcagc cacatcaatt ccttggtcat tgcgacaccc
     2341 cgtttgacgc ttcgccctgc gcaagcatag tggaaagggc cgtggctgtc gcgcccgatg
     2401 ggcgtcccaa ggcgtaaccg gagttcgtgc tagtctgggc caggagttca caggatcgcg
     2461 ccatggccgc atccgccaaa cccgagatca aggcgttttt cgacgaacca accaatatgg
     2521 tgagctacct ggtctcggat ccggcgacca gagaaggcgc cgtgatcgat ccggtgcttg
     2581 atttcgatcg tcgcagcggt gacgcaacgg tgaaatccgc agatgaaatt ctggctgatg
     2641 caaaaaggct cggcgtcgcc atcacgcgaa ttcttgagac gcgcgcccac gctgatcatc
     2701 tttcggcgcg cactatctga aggtgccggt gaaaatcgtc tcttggcgca gagtcaggtc
     2761 cagctcttca ccttgacgta agacccgggg gcatcttcga tcggcttgag cttgccgcgg
     2821 ccgattttgc gggccgccac ggtttcggtg ccttgcgctt tgagccaagc gagccagtca
     2881 ggccaccacg atccggggtg ttccgtggcc gactccatcc aggcttcaac gctgtcgccg
     2941 tttggcttgc cgcccgtcca gtactgatac tttggtttgc cgggcggatt gatgacgccc
     3001 gcgatgtggc cggagccggc gagcacgtat ttgaccggac cgccgaaaaa ttgcgagccg
     3061 tagagcgccg atttcgccgg cgcaatgtga tcttcgcgcg tagccaggtt gtaaaccggc
     3121 actttcaccg tgtgaaggtc gaggcgggtg ccggcaattt ccatttcgcc tttggtgagc
     3181 ttgttgtcga ggtagcaatt gcgcaaatag aaagaatggt tggccgcggg catgcgggtg
     3241 gcgtcggaat tccagtagag aatatcgaaa ggcaacggtt tcttgccgcg catgtagttg
     3301 ttgacgatgt aaggccagat caaatcgttt gagcgcaaca tattgaaggt cgtggccatg
     3361 cgggacgctt ccagataacc ttgctccttc atgtgcgtct cgagtcgcct gatacgctct
     3421 tcatcgacga acaccatgag gtcgcccgcg taggtgaaat cgatctgcgc tgcaaacaat
     3481 gtcgccgatt tgacgcggtt ctgctttttt gcggcgagat aggcgagggt gatggaaagc
     3541 agcgtgccac cgacgcagta gccgatggta tgcgtcttct tctcgccggt gatggcttca
     3601 atcgtatcga gggcggtgat tacgccctcg cgcatgtatt cttcaaaact ttttgcggcg
     3661 agtcttgcgt ccgggttgac ccaggaaatt acgaacaccg taattccctg atcaacgcac
     3721 catttgatga aggatttttc cggcgctaaa tcgaggatgt agaacttgtt gatccagggg
     3781 ggcacgatca gcagcggcag cttcaggacg ttcttggtgg tcccctcata ttggatgagc
     3841 tgcatcaatt cattctgaaa caccactttt ccgggcgtcg tggcaagatt tttgcccaca
     3901 tcgaacatgg aagggtcgga ttggcggatt ttgagaaccc cttggccggc ctttatgtct
     3961 tcagaaagca tgtgcatccc gcgcacgaga tttccggcgt tcgaagagag tgtctcgcgc
     4021 agcaattccg gattggtcag cacgaagttt gacggcgaaa tcgcattggc gatttgcctg
     4081 acgtaaaatt ccgccttctt gcgcgtgtgc tcgtcaactc cggccgcgtc cttcaccagc
     4141 ttatccgccc aatgtgcggt aagcagatag gcctgcttca ggaaatcaaa gaactggttt
     4201 tgcgaccatt ccggatcgga aaagcgcttg tctctcggat ctggcgagac gacgggttcg
     4261 gcttgctccc ccgccatgcg cttcacggcg ccggcccaga gttcaaggta agcgcggcca
     4321 aggctggtct gaagctcgat cgcacgccgg ggatcggaca tccaatactc gacgacgcgg
     4381 ccgaccgtct tgactgcatc ggtcaggtcc tcggcgtgat cgttgcgtat tttgccttcc
     4441 tcgcgcggtt tcaggtatgc cgcgagcgcc ttgccgcctt cctcgatcag gcgcgcgaga
     4501 ttgcgcgaga gcgcctcgat atcgacgtcg ccgaccttgg caggttctgt ggtggaccta
     4561 tccatccttt ccgctcccct ctgccaacac agttggcccg cggcgcattc catcgcaagg
     4621 cgcgtttgcg cgcatggttt cgcccgtctt cagccgagcg gttagcaaca cgtgaccttg
     4681 ctgcagccat attagcccgt taacggcacg gtgatataat tcgttgagtc tgcatcgatt
     4741 tcgcccactt gtgcagggcg gcagcggagc aagtgcgtgt cggtcaaata cttcctatgg
     4801 gcggggttga gcttcgccac ggtgctgacc ggatgcgctc ccgggccggt gattgaccgt
     4861 ctgcctggtg acctgggctt gcctgccgcc gctccggccc gcccggaaac gccatacgaa
     4921 taccccgccg tgcacgatat gccgcctgat cgcgcatcat cccccatgac agaggaagag
     4981 caggtcaggc ttgaaaagga attgatcaag gtccgcgatc gccaggaggg ccaaccgccc
     5041 gcaaccaaaa aaggcgctcc agcagcaaaa aaaccgccca aagacacgca aaacgcccaa
     5101 accgatggcg ccaaggggaa gccgtgataa aagccagccg gggatatgcg gcggctggtg
     5161 agggcagccg tcctttgagg ttttgagccg atggaagagt tctaccgcat tcgccgtttg
     5221 ccgccttatg tgttcgaaga ggtgaacaag gccaaggcga aggcccgcaa cgcgggcgcg
     5281 gacatcattg atcttggcat gggcaatccg gatttgccgg cgcctgcgca cgtcatcgaa
     5341 aaactcaagg acacgctggg caagccgcgc accgaccgct actcggcgtc gcgcggcatt
     5401 cccggattgc gtcgcgcgca ggctgcctac tatgaccgcc gctttggcgt gaagctcaat
     5461 cctgacactc agattgtcac cacgctcggc tccaaagagg ggttcgccaa cgtggcgcag
     5521 gcaatcaccg cgccgggcga cgtcgtcctg gtgccgaacc caagctaccc cattcatgcg
     5581 ttcggtttcc tgatggcggg tggggcaatt cgctcggtcc cctcggagcc gacgccgaat
     5641 ttctttcata ccctcgagcg cgcgatcgtc cactccatcc ccaagccgac cgcggttgtg
     5701 gtctgctatc cggcaaaccc gaccgcctat gtagccacgc tcgatttcta caaggacctc
     5761 gtcgcgttcg cgaagaagca cggcattctg gtgctgtcgg atctcgctta tgcggaggtt
     5821 tattttgaca ataatccgcc gccgtccttg ttgcaggtac cgggcgcgaa tgacattgcg
     5881 gttgagttca cctctatgtc gaagacgttc tcgatgccag gctggcgcat tggctttgcc
     5941 gtcggcaatg agcgcgtcat tgcggcactg agccgcgtaa aatcctatct cgactacggc
     6001 gcgttcacac cggtgcaggt agcggcaacc gccgcgctca acggccccga cgattgcgtc
     6061 aaagagatgc gggcgactta caggcgtcgt cgtgacgttt tggttgaaag cttcgggcgc
     6121 gcaggctggc acgttccggc gccgcgcgcg tcgatgtttg cgtgggccgc gataccggaa
     6181 cctttcaaca cgctcggaag cgtggagttt tccaagttgc tggtcgagaa ggccgatatc
     6241 gcagtgtcgc ctggcattgg tttcggagaa tatggcgaag gctatgtccg tattgcgctc
     6301 gtcgaaaacg agcagcgtat ccgccaggct gcgcgcaacg ttcgccgctt tctcgaccat
     6361 gcgcccgagc aattgcacaa cgtcgtcccg ctcgcgacgc ggcgctaaca acaggcgcgc
     6421 acgttcatgg caccaccgct gaaagtgggc gttgccgggt tgggcacggt cggtgctgcg
     6481 ctgcttgcgc aagtcgcgcg ccaac
//
LOCUS       DASSRL010000452         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_29219, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000452 DASSRL010000000
VERSION     DASSRL010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29219"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFL49_09205"
     CDS             <1..470
                     /locus_tag="VFL49_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7912198.1"
                     /translation="ATLDIFRSTVAAAALGLARRGLDEMMERAANRNLFGTPLADLQL
                     TQAAIADSAIDIDAAALLVYRAAWEKDQGALRITREASMAKAFATEMAQRVIDRAVQI
                     FGGNGVRVGTKVEALYREIRALRIYEGATEIQKLIVARELLKSREVMRRQAAE"
     gene            476..1633
                     /locus_tag="VFL49_09210"
     CDS             476..1633
                     /locus_tag="VFL49_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7912199.1"
                     /translation="MLLTEEQEQIRNTAREFAQRVLAPSSAEWDRQAEFPRDALTKMG
                     KLGFLGMTVPPEWDGVGADYVAYAAALEEIAAGDGAVSTIMSGHNSVGCMPLVEYGTQ
                     KQKERYLRPMARGELLSAFCLTEPQGGSDASAIRTRAERRHGTYILNGTKQFITSGKN
                     ADIALVFAATDFGLGKQGISCFIVETNQTGYCVTRVESKMGQHASDTCEIRFDELSVP
                     MENRLGAEGQGYRIALANLEGGRIGIAAQAVGMARAAFEIAVRYAQERETFGKVIIEH
                     QAVAFRLAEMATQLEAARELVHYAAALRSQGLPCLKQASMAKLYATEAAERICSDAIQ
                     TLGGAGYMTGHHVERIFRAVRASKIYEGTNDIQRLVISRALIDETQQAKEV"
     gene            1636..2430
                     /locus_tag="VFL49_09215"
     CDS             1636..2430
                     /locus_tag="VFL49_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7912200.1"
                     /translation="MFTVETNNAVTTLTLSRAPVNAISEEWVHSFNAILDALTAEPRS
                     AVLHIRSDQKVFCAGADLKEVRARMDAPDGADRMYAYVAGIQRLYARIERLPQVTVAE
                     IGGAAMGGGLELALASDLRIAASEAKLGLPEVGLGLIPGAGGTQRLARLCDPALAKRL
                     ILRAEIIDGFTAERLGVVHWAVPRAGLAQRATEIVRDIARLPAEALAASKACIAAAGN
                     SGSGGYTDELEFTRLLLTNTDTRKRVEEFLAGADRSSVQMKRGAAR"
     gene            2427..3173
                     /locus_tag="VFL49_09220"
     CDS             2427..3173
                     /locus_tag="VFL49_09220"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7912201.1"
                     /translation="MRFDDKVVLVTGGASGIGKATVMAFARAGATVICADINAAKSAE
                     LKREAAGTNLKVDFAAIDMANPDTIRAAVAELLKRYSRVDILVNGAGWGDIQPYIQNS
                     ADFINRVIAINFAGPAHLTQALLPPMIAANGGKIINIASDAGRVGSSGETVYAGAKGG
                     LIAFTKSLARELARHSINVNCVCPGPTDTPMLATRPEKLREAFLKAIPFRRFAKPEEI
                     ADAVMFFASPQADYVTGQVMSVSGGLTFAG"
     gene            3241..>3773
                     /locus_tag="VFL49_09225"
     CDS             3241..>3773
                     /locus_tag="VFL49_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7912202.1"
                     /translation="MTAVAKVGDLGSGVTAVRFRPAGLDFSNFKPNHFLWKVDGKVAT
                     ITLNRPERKNPLTLESYAELRDTFRNLAFADEIKNIVIAGAGGNFCSGGDVHEIIGPL
                     VEMQRKDDMAGLLAFTRMTGQLVKEMRACPQPIVAAIDGICAGAGAIIAMASDLRVGT
                     ARSKVAFLFVRVGLAGA"
BASE COUNT          823 a         1021 c         1169 g          760 t
ORIGIN      
        1 tggcaacgct cgacatattt cggtcgaccg tggccgccgc ggcgctcggc ctggcgcgtc
       61 gtggactcga tgagatgatg gagcgagccg caaatcggaa tttgttcggg acaccgctgg
      121 cagatttgca gctcacgcag gcagcgatcg ccgacagcgc aatcgatatc gatgctgcag
      181 cgctgctcgt ctaccgtgcg gcgtgggaaa aggatcaagg ggcgttgcgc atcacgcgcg
      241 aagcctcgat ggcgaaagcc tttgcgactg agatggcgca acgggtcatc gatcgcgccg
      301 ttcaaatatt cggcggaaac ggcgtgcgcg tcggcaccaa ggtcgaggcg ctctaccgtg
      361 aaattcgagc gctgcgcatt tacgaaggcg caaccgaaat tcaaaaactg atcgtggcgc
      421 gggaactact gaaatctcgc gaagtgatgc gccgacaggc agcagagtaa agctgatgtt
      481 gcttaccgaa gaacaggagc aaattcggaa taccgcgcgc gagtttgcgc agagggtctt
      541 agcgcccagt tcagccgaat gggatcggca agcagaattt ccccgtgacg cgctgaccaa
      601 aatgggaaag ctcggttttt tgggaatgac ggtccctcct gaatgggatg gagtcggcgc
      661 agactatgtc gcatatgccg ctgcgcttga agaaatcgcg gcgggcgatg gggccgtctc
      721 gacgatcatg agcggccata actccgtcgg gtgtatgccg ctcgttgaat acggcacaca
      781 aaaacaaaag gaaaggtatt tgcgcccgat ggcgcgcggt gaacttctga gcgctttttg
      841 tctcacggaa ccacagggcg ggtcggatgc gagtgctatc cgaacacggg ccgagcgccg
      901 acacggaacc tacattctta acggaacgaa acagtttatc acgtccggca aaaatgccga
      961 catcgcgctg gtgtttgccg ccacggattt tggactgggt aaacaaggta tcagctgttt
     1021 catcgtcgag acaaatcaaa ccggttactg cgtgacccgg gtcgaaagta aaatgggaca
     1081 acatgcgtcg gacacatgtg aaatcaggtt cgacgaactc agcgtcccga tggaaaacag
     1141 gcttggtgcg gaaggtcaag gttatcgaat agcgcttgcc aatcttgagg gaggccgcat
     1201 cggcatcgcg gcgcaggctg ttggcatggc gcgagccgct tttgagatcg cagtccggta
     1261 cgcgcaggag cgcgaaacct ttggaaaagt gatcatcgag catcaggccg ttgccttccg
     1321 tcttgccgaa atggcgactc aacttgaggc cgcgcgcgaa cttgtgcatt acgccgctgc
     1381 tttacgaagc caaggattgc cgtgtctcaa acaggcttcc atggcgaaac tgtatgcaac
     1441 tgaagcggcg gaacgcatct gttctgacgc aattcagacg cttggcggtg ccggttatat
     1501 gaccggtcac catgtcgaac gaatttttcg tgcagttcgc gcgagcaaaa tctacgaggg
     1561 aacaaacgac attcaacggc tggttatcag ccgcgcgctc atcgacgaga cccaacaggc
     1621 aaaagaggtc tgacaatgtt cactgtcgag accaacaatg cggtgacaac cctgacactg
     1681 agccgcgcgc ccgtgaatgc gatcagcgag gaatgggtgc attcattcaa cgcgatactt
     1741 gacgcattga ctgcggagcc gcgatcggcg gtcctgcata ttcgttctga tcaaaaggtc
     1801 ttctgcgccg gcgccgatct caaggaagtg cgtgcgcgta tggacgcgcc tgatggcgct
     1861 gatcgtatgt acgcctatgt cgcgggtatc cagcggcttt atgctcgaat cgagcgcttg
     1921 ccgcaggtta cggtggccga gattggcggc gcggcgatgg gtggggggtt ggaactcgcg
     1981 ctggccagcg atctgcggat cgccgcgagc gaagccaagc tcggattgcc ggaagtcggc
     2041 ttgggattga ttcccggtgc cggtggcact cagcgcctcg cacggctatg cgatccggcg
     2101 ctcgcgaagc gcctcattct ccgagccgag atcattgacg gcttcactgc ggaaagattg
     2161 ggcgtagtgc attgggcggt gccaagggcc ggcctggcac agcgggcaac ggaaatcgtg
     2221 cgcgatattg cgagactacc cgcggaggcc cttgctgcca gcaaggcgtg catcgcagca
     2281 gccggcaata gtggctccgg tggatataca gacgaactcg aatttacccg tctgctcctc
     2341 accaacaccg atacgcgaaa acgcgtcgag gagtttcttg ccggtgcgga cagatcttct
     2401 gttcagatga aaaggggagc ggcacgatga gattcgatga caaggttgtc ctggtcaccg
     2461 gcggcgcctc cggcatcggc aaagcgacgg tgatggcgtt cgcgcgcgcc ggtgcaaccg
     2521 ttatctgcgc cgacatcaat gctgcaaaaa gcgccgagtt aaagagggag gctgccggta
     2581 ccaacctcaa ggttgatttc gccgccatcg atatggcgaa tcctgatacg atccgagctg
     2641 ccgtcgccga actgctgaaa agatattcac gggttgacat tttggtgaat ggcgccggct
     2701 ggggagatat tcagccgtac attcaaaatt ctgcggattt catcaaccgc gtgatcgcga
     2761 tcaatttcgc cggtccggca catctgactc aggcgctatt gccaccaatg attgccgcca
     2821 atggcggcaa gatcatcaac attgccagcg atgctgggcg ggtcggcagc agcggtgaga
     2881 cggtctatgc cggcgccaaa ggtgggctta ttgctttcac caagtcgctc gcgcgcgagt
     2941 tggcccgaca ttcgatcaac gtcaattgtg tatgccctgg tccgactgac acgccgatgc
     3001 tcgcaactcg cccggagaag ctgcgcgagg cttttcttaa ggcgatcccg ttcagacgct
     3061 ttgccaaacc agaggaaatc gccgacgcgg taatgttctt cgccagcccg caagcggact
     3121 acgtgaccgg gcaggtgatg agcgtgagcg gtggccttac ctttgcggga tagcgcgaga
     3181 cctcaaaaat agggtcggac ttccgcaaaa tatcggcgta atgcaagaat ggaggtgtcg
     3241 atgactgcag ttgcaaaggt tggcgatttg ggttccggcg tcacggctgt ccgctttcgc
     3301 cccgctgggc tcgatttttc aaatttcaag ccaaaccact ttctctggaa agtcgatggc
     3361 aaggttgcga ccattacttt aaaccggccg gaacgaaaga atccgctgac attggagtct
     3421 tatgccgagt tgcgcgacac gttccgcaac cttgcctttg cggacgaaat taagaacatt
     3481 gtgattgccg gcgccggcgg caacttctgt tccggcggcg acgtgcacga aatcatcggg
     3541 ccgctggtcg aaatgcagcg caaagatgac atggcagggc tgctcgcttt tacccggatg
     3601 acgggtcagc ttgtgaagga gatgcgggct tgcccgcagc ccatcgttgc ggcgatcgac
     3661 ggcatttgcg ccggcgccgg cgcaattatt gccatggcgt cggatttgcg tgtcggcacc
     3721 gcacgcagca aggtcgcctt cctctttgta cgcgtcggcc ttgccggggc cga
//
LOCUS       DASSRL010000453         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_711702, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000453 DASSRL010000000
VERSION     DASSRL010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711702"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFL49_09230"
     CDS             <1..527
                     /locus_tag="VFL49_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7912203.1"
                     /translation="CSKTMLQIVNLPRPVIAAVQGVATAAGCQLVASCDLAVASTAAK
                     FATPGVDIGLFCSTPMVALSRNIARKQAMEMLLTGDAITAENACRIGLINRVVTPGTE
                     REEALAFARKIGSKSSHIVKIGKEGFYRQAEMNLSSAYQYVSEVMVENLMARDAEEGL
                     KAFIEKRKPKWEDC"
     gene            complement(612..1034)
                     /locus_tag="VFL49_09235"
     CDS             complement(612..1034)
                     /locus_tag="VFL49_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912204.1"
                     /translation="MCDYSLHDVASRPAKVGDKLISTQFPNAITRGFADPAEPRVAVC
                     LMPGTELAFENDVECDASFRFLPTRKSTHKVARFRQVNLNRPAAHHDALEFPDGKIVL
                     VTDLCEGQHATVLQLPSAGRPAEEHGESERAPNESLIA"
     gene            1225..1851
                     /locus_tag="VFL49_09240"
     CDS             1225..1851
                     /locus_tag="VFL49_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7912205.1"
                     /translation="MNNSSLVFTPAAQQAQLERGSAKAYARRMAEGFPDRVTPELAKF
                     IAEQDTAFLATASGAGAPYLQHRGGPRGFIKVIDEKTLGFADYRGNRQYITLANLSEN
                     DRAFLFLLDPARRQRIKIWGRARMVEGDPALVGRLFDDGYKAKPERAILFTIEAWDVN
                     CSSHIVTRFTEAEIEEAFAAVQARIAELEAENARLRAAVEKQPAIPSE"
     gene            1848..2393
                     /locus_tag="VFL49_09245"
     CDS             1848..2393
                     /locus_tag="VFL49_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7912206.1"
                     /translation="MNHDSYADSYIRGILNTVKTIAMVGISPKDNRPSYFAFKYLLER
                     GYRMIPVNPGQAGKEILGQMVYAKLADIPEPIDMVDIFRSSASVPPVVEEALGLEPKP
                     RVIWMQLTVRNDEAARRAEEAGMKVVMNRCPKIEYGRLSSEISWMGVNSRMLSSKRPQ
                     TLGGGVQRMRLGPASDHDNDP"
     gene            2512..>3280
                     /locus_tag="VFL49_09250"
     CDS             2512..>3280
                     /locus_tag="VFL49_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7912207.1"
                     /translation="MGDVERNPGFATLAVHAGAKPDPTTGARATPIYQTTSFVFDDVD
                     HAAALFGLQTFGNIYTRIGNPTCAVLEERVAALEGGTAGLAIASGHAAQVVVMHALMT
                     PGDELVASKKLYGGSINQMNHSYKNFGWNVVWADPDDLASFEKAVTAKTKCIFIESIA
                     NPGGVITDIEAISRIARKAGIPLIVDNTLATPYLCKPIDFGADIVVHSLTKFLGGHGN
                     SIGGLIVDCGTFDWSRDKKYPMLCEPRPEYQGIVLHET"
BASE COUNT          676 a         1005 c          967 g          632 t
ORIGIN      
        1 tgtgcagcaa gacgatgctt caaatcgtca atttgcccag gccggtgatt gccgccgtcc
       61 agggcgtcgc gactgcagcg ggctgccagc tcgtcgccag ctgcgatctc gctgtcgctt
      121 ccacggctgc aaaattcgcc acgcctggcg tggacattgg cctgttttgc tcgacgccta
      181 tggtcgcgct ctcacgcaac atcgcccgca agcaggccat ggaaatgctg ctcaccgggg
      241 acgccattac ggcagagaac gcctgccgca tcggtctgat caaccgcgtg gtcaccccgg
      301 gcacggaacg cgaggaggcg ctcgccttcg ctcgcaagat cggaagcaag tccagccaca
      361 tcgtaaaaat cggcaaggaa ggtttctatc gccaggccga gatgaatttg agcagtgcct
      421 accagtatgt ctcggaagta atggttgaaa acctgatggc acgcgatgcc gaggaaggat
      481 tgaaggcgtt tatcgagaag cgaaagccga aatgggaaga ctgctgactg cgcgcatcgc
      541 atcaagccag tcaaagctca tcgagagcca ccggcagacg gtggctctct taatttcttg
      601 tggcacaagc cttatgcgat aagggattcg ttcggcgccc tttcgctctc gccgtgctct
      661 tccgccggcc gccccgcgct cgggagctgc aatacagtcg cgtgctggcc ttcgcaaagg
      721 tccgtcacca ggacgatctt cccgtccggg aattccagag cgtcgtggtg agccgccggc
      781 cgattgaggt tgacctgccg gaaacgtgca accttgtgcg tcgacttacg tgtcggcaaa
      841 aaccggaacg aagcgtcgca ttcgacgtca ttttcgaatg caagctcggt gcccggcatc
      901 aggcacacag cgacacgcgg ctcggccggg tcggcaaagc cgcgcgtgat ggcattggga
      961 aattgtgtcg agatcagctt gtcaccgacc ttggccggcc gggacgcaac atcgtgaaga
     1021 ctgtagtcac acattttgaa cgcctcctgt ttgtgcacaa cgtaggcggc gcccctcagc
     1081 cgtcggatag cagcaaacca gcgtgacctt cttatgtcgc cgcctaccgc aatcctgccc
     1141 aattcaattc gcgcggccct atcgagagca acagcgaaag actatctttg ttcccgctcg
     1201 ggacagcgat cgggtccgtc acaaatgaat aactccagcc tggtattcac cccggccgcg
     1261 cagcaggctc aacttgagcg cggctccgcc aaagcctatg cgcggcgtat ggcggaaggc
     1321 tttccggacc gggtgacgcc ggaattggcc aaatttatcg ccgaacagga taccgccttt
     1381 cttgcgaccg cgagcggtgc tggtgctccg tatttgcagc accgtggcgg gccaaggggc
     1441 tttattaaag ttatcgatga aaagacgctc gggtttgccg actaccgcgg caaccggcaa
     1501 tacatcacgc tcgccaacct gtcggagaac gatcgcgcat ttttgtttct gctcgacccc
     1561 gcgcgccgtc agcgaatcaa gatctggggc cgggcccgga tggtcgaggg tgacccggct
     1621 ctggtcgggc ggctgttcga cgacggctac aaggccaagc ccgagcgcgc aatcctgttc
     1681 acgatcgagg cctgggatgt gaactgctct tcacatatcg tcacgcggtt cacggaggcg
     1741 gagatcgagg aagcatttgc cgctgttcag gcccggatcg ccgagttgga agccgagaac
     1801 gcacgcctgc gggcggccgt cgaaaagcaa ccggctatac cctcagaatg aaccacgaca
     1861 gctacgccga cagctacatt cgcggtattc tcaacacggt gaagaccatc gccatggtcg
     1921 gaatatcgcc gaaggataat cggccgagct actttgcctt caagtatctg ctcgagcgcg
     1981 gctaccggat gatccccgtc aatccgggcc aggcgggaaa agaaattctc ggtcagatgg
     2041 tctacgcaaa gctcgccgac atccctgaac cgatcgatat ggtcgacatc ttccggagct
     2101 cggcgtctgt gccgccggtc gtagaagagg cgctcggcct cgagccaaag cctcgggtga
     2161 tctggatgca actcacggtc cgaaacgacg aggcggcacg gcgcgcggag gaagcgggca
     2221 tgaaagtggt catgaatcgc tgcccgaaga tcgaatatgg ccgcctttcc tcggaaattt
     2281 cctggatggg cgtgaactcg cgcatgcttt cttcgaagcg cccgcagacg ctgggcggcg
     2341 gcgtgcaacg catgcgtctt gggcccgcat ccgaccatga caacgatcca tgaggccgtt
     2401 ctacgcctgg gcgggccgta ctgccttgac gccacccctg tccggccgca ctaatcgccg
     2461 catcattcgg cgggacggcc tgcttcattt tatggcgcga tcggaggtct aatgggcgac
     2521 gttgaacgca acccaggctt tgccacgctg gcggttcacg cgggggccaa gcctgatcca
     2581 accaccggcg cgcgcgcgac gccgatttat caaaccacgt catttgtgtt cgacgacgtc
     2641 gatcacgccg ccgcgctgtt cggcctgcag accttcggca atatctacac ccgtatcggc
     2701 aatccgacct gcgccgtgct ggaggagcgc gttgcagcgc tcgagggcgg cacggccgga
     2761 ctcgccattg cctccggcca tgcggcgcaa gtcgtcgtga tgcacgccct gatgacgccg
     2821 ggtgacgaac tggtcgcctc gaaaaagttg tacggcggtt caatcaatca gatgaaccat
     2881 tcttacaaga atttcggctg gaacgttgtg tgggcggacc ccgacgatct ggcctcattc
     2941 gagaaggctg tcacagcgaa gaccaaatgc atcttcatcg agtcgatcgc aaatcccggc
     3001 ggcgtcatca ccgacatcga ggccatttcc aggatcgctc gaaaagcggg cataccgctg
     3061 atcgtcgaca acaccctcgc aacgccctat ttgtgcaaac cgatcgactt cggagccgat
     3121 atcgtcgtgc actcgctcac caagtttctc ggcggtcacg gcaattcgat cgggggactg
     3181 atcgtcgact gcggcacctt cgattggtcg cgtgacaaga aatatccgat gctctgcgag
     3241 cctcgccctg aatatcaggg gattgtcctg cacgaaacct
//
LOCUS       DASSRL010000454         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1709199, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000454 DASSRL010000000
VERSION     DASSRL010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709199"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFL49_09255"
     CDS             <1..397
                     /locus_tag="VFL49_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET7912208.1"
                     /translation="DVTIVDPRTAFASIERFPDVKVIAEWPDVALPPLGIDHYTAFVA
                     LTHDPKIDDPALKHALARDCFYIGALGSKKTHARRVERLKKEGLSDNDIGRIHAPIGL
                     DIDAVSPAEIAVAIMGEITQVLRQKAEAA"
     gene            394..2016
                     /locus_tag="VFL49_09260"
     CDS             394..2016
                     /locus_tag="VFL49_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HET7912209.1"
                     /translation="MKFGSVPVAEAIGGTAVHSIRQGDFVLKKGTLISSAEVAALKTA
                     GVKEITVARLEPGDVSEDQAAADIAAAIAGAGVRTDRAFTGRCNLFAAGAGILVVERE
                     AVDALNRIDEAVTLATLPAYKPVVEGEMIATVKIIPFAVARAARDQAVAAAAKARPVI
                     RVAPYKLRKIGVVSTLLPGLAPKVIEKTLKITAARIAPAGASIIAEKRVPHEQAVLAT
                     AIDELLKAGADLVIVFGASAIADRRDVIPSAIEGVGGDIEHFGMPVDPGNLMLIGKAR
                     GHPVIGAPGCARSPKENGFDWILMRLLAGLPVTRADVTGMGVGGLLMEIVTRPQPREE
                     RTPQTGRRVAALVLAAGRSTRMGAVNKMLAEIGGKPLVRIAAEQAVASHAQPVIVVTG
                     HERERVETALKDLPVRFVHNQDYAEGLGTSLKAGVAAVPDDADAAIVCLADMPQVDAA
                     LINRLINAFDPERGALVIVPSIDGRRGNPVVWSRRFFHDLMAIHGDVGARHLIGNYAE
                     AVVEVPVAGEAALTDVDTPESLLAVKAEIERA"
BASE COUNT          396 a          651 c          703 g          398 t
ORIGIN      
        1 cgacgtaacg atcgttgatc cgcgcaccgc ctttgcatcg atcgagcggt tccccgacgt
       61 gaaagtgatc gccgaatggc cggacgtggc attgcctccg ctcggcatcg atcactacac
      121 ggcctttgtg gcgctgacgc acgatccgaa aatcgacgat ccggcgctca agcatgctct
      181 tgcgcgtgat tgcttctaca tcggtgccct gggttccaag aagacccatg cccggcgtgt
      241 cgagcggctg aagaaggagg gactgagcga taacgacatc ggccgcatcc atgcgccgat
      301 cgggctcgat attgatgcag tgtcgcccgc ggaaattgcg gttgcgatca tgggcgaaat
      361 cacgcaggtg ctgcggcaga aggcggaggc ggcatgaaat tcggatctgt ccctgttgcc
      421 gaagccatcg gcgggaccgc agtgcattcg atccgccagg gtgatttcgt cctcaagaaa
      481 gggaccctta tcagctctgc tgaagttgca gccctcaaga cggcgggcgt aaaggaaatc
      541 accgtagcgc gcctcgagcc gggcgacgtc tcggaagatc aggcagccgc tgacattgcc
      601 gcggcgatcg ccggcgcagg tgtacgtacc gatcgggcat ttacgggccg ctgcaatctt
      661 ttcgccgcgg gtgccggaat cctggttgtc gagagggagg cggtggacgc ccttaatcgg
      721 attgatgagg cggtcacact ggcgacgctg ccggcctaca agccggtggt cgaaggcgaa
      781 atgattgcaa cggtgaaaat tattccgttc gccgtggcga gggccgcgcg cgaccaagcg
      841 gtagccgccg cggcgaaagc cagaccggtc attcgggttg cgccctacaa gctgcgcaag
      901 atcggtgtgg tgtccacgct gttgcccggt cttgctccga aggtcatcga aaaaaccttg
      961 aagattaccg ctgcgcgcat tgcgccggcc ggggcgtcca ttattgccga aaagagggtg
     1021 ccgcacgagc aggccgtgct tgcgaccgca atcgacgaac tgctcaaggc gggtgccgac
     1081 cttgtcatcg tgttcggcgc gtcggccatt gcagatcgcc gtgacgttat tccttcggcc
     1141 attgagggag ttggcggcga tatcgagcat ttcggcatgc cggtcgatcc gggaaacctg
     1201 atgctgatcg gcaaggcgcg cggtcaccct gtgattggcg ctcccggttg tgcgcgcagc
     1261 ccgaaggaga atggtttcga ctggatcctg atgcggcttc tcgctggact gcctgtcacg
     1321 cgcgcggatg tcaccggcat gggcgtcggc gggctgctga tggagatcgt gacgcgcccg
     1381 cagccgcgcg aagaacgcac accgcagacc gggcgccgcg ttgccgcatt ggtgctggcg
     1441 gcaggacgct ccacgcgcat gggcgccgtc aacaagatgc ttgccgaaat cggaggcaag
     1501 cccctggtgc gcattgcagc cgagcaggcg gtcgcttcgc acgcccaacc tgtcatcgtt
     1561 gtgaccggtc acgaacgtga gagggtggaa accgcgctca aggacctgcc ggtccgcttc
     1621 gtgcacaatc aggattacgc ggaaggtctt ggcacctcac tcaaggccgg cgttgccgct
     1681 gtccccgacg atgccgacgc agcgatcgtc tgcctcgccg acatgccgca agtcgacgcc
     1741 gccttgatca accgattgat caacgcattc gatccggaac gcggcgcgct cgtgatcgtg
     1801 cccagcatcg acggcaggcg cggcaatccg gtggtgtggt cgcgccgctt cttccacgac
     1861 ctgatggcca ttcacggaga tgtcggtgcg cgccacctga tcggcaatta tgccgaagcc
     1921 gttgtggaag tgccggtagc cggcgaggcc gcactgaccg acgtggacac tcccgagtcg
     1981 cttctggccg tcaaggccga aattgaacgc gcctgagatt cgaatcattg atcgacccgg
     2041 acgactcact cctagctgag cgaggcagca gggaaaggcg atgcttcacg tctatgcccc
     2101 gatgatgcgc gcgcttgtgg tcctggcctt ggtgctcgcc acggccac
//
LOCUS       DASSRL010000455         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_222125, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000455 DASSRL010000000
VERSION     DASSRL010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222125"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFL49_09265"
     CDS             complement(<1..450)
                     /locus_tag="VFL49_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7912210.1"
                     /translation="MRKPLDFRFDEFEIDIARRELRRRRELVPLEPQVFDLLVHLVRN
                     HSRIVSKNELIEVIWNGRAISDAALSSRVSAARRAVGDSGADQKLIRTLNKRGFRFVG
                     AVQMAGGKPQTSSDWRRSESSALAWSDRASIAVLPFQNISGDPDQEYL"
     gene            complement(1171..>2230)
                     /locus_tag="VFL49_09270"
     CDS             complement(1171..>2230)
                     /locus_tag="VFL49_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HET7912211.1"
                     /translation="NGGFNPRDVAEPALFLTRWVTHFCAPTFIFLAGISAYLYGVKGR
                     STGDVSRFLLTRGIWLVLIEFTVVRLGWSFDLHFNYFVAQVIFAIGVSMIALAAFVHL
                     PRKVVAAIGLAMIVGHNAFDGITATQFGAAAPLWNVLHQPGLFDIVPGVKFFVLYPLI
                     PWIGVMAAGYALGPVFLQGRTGRRRRLFMLGAALTIGFVLLRATNFYGDPVPWTPQDN
                     AVSTVLSFINCEKYPPSLLYLAMTLGPALMLLAAFESARGPVAVWVTAFGRVPFFYYV
                     VHIFLLHALALLFAWLSIGQIGFLFGPSAGHKPATYGLGLAGIYAVWLGVVVALYPLC
                     RWFAGIKRRHTGWWWSYL"
BASE COUNT          518 a          665 c          676 g          371 t
ORIGIN      
        1 aagatactcc tggtcggggt cgccgctgat attctgaaac ggcaagacgg caatcgaagc
       61 cctgtcggac cacgcgaggg cggaactttc agacctcctc caatcggatg aggtttgcgg
      121 ctttccgccc gccatctgga ccgccccgac gaaccggaaa ccgcgcttgt tgagcgtgcg
      181 gataagcttt tgatcggcgc cgctgtcgcc gaccgccctc cgcgccgcgc tcacacgcga
      241 gctcaacgca gcgtcggaaa tagcgcggcc attccagatc acctcaatca gctcattttt
      301 gctgacgatt cggctgtggt tgcggacaag gtgcacgagc agatcaaaaa cctgaggttc
      361 gagcggcacg agctcgcgcc gccgccgcag ctcacggcgc gcaatatcga tttcaaactc
      421 gtcaaaccgg aagtccaagg gctttctcac gttgcaagcc gcagacaccg cgggcctaac
      481 atcatgaaat ggctgcggtg tgtgatgggc atcacattcg ccgcgataat ccgagacggc
      541 ttttgcagaa aaagaggccg ccatcgcagg cggcctcatg gcaatcaggc ttgctcggga
      601 ggggagatta tcagctctga ctgccaattc cgaaatccac accgccagcg gcgggcaagc
      661 gggccggcgc cggcgcgtag gctctcgagt cacttgcacg gtgctggacg gcgcgtttgc
      721 ccgcggcctg atgctcgcta gaatgcatgc ttgacacgga cgggacgctt tcaagatcgt
      781 tctcgtacgc aaatgccgtg ccgacggtcg acagcgcaag cgcggaggcg aatacaagtt
      841 tggtgagatt tttcacggtt cagctccgtt gtttgggcaa gatgaatgtc ttgcgggcgc
      901 ggcttgtggc gcaaagtcgt gtcatgctca agatgctacc gcatcacaaa ttttcggctt
      961 tcgttcacgc aaatgtcatg gagtatcggc gcgcacagaa agtgactgac attcaactcc
     1021 tcgagcagcg cgccgatgca gaaccaggac tcacgcgaca gagtcattcc tgcaaatcaa
     1081 tgttgccatg cgttgcgcgc gttcgagacg aacggcagcg acagttttta tcgatttgaa
     1141 tgatcgccac gatcaatcaa attgaagcgg ttagaggtag ctccaccacc atccggtatg
     1201 gcgacgtttg attcccgcaa accagcggca caggggataa agcgcgacaa caaccccgag
     1261 ccagaccgca tagataccgg cgaggcccag tccgtaggtg gcgggcttgt gtcccgccga
     1321 tggcccgaac agaaaaccga tctggccgat cgacagccag gcaaacagca gggcgagggc
     1381 gtgcagcagg aagatgtgca ccacgtaata gaagaacggc acgcggccga aggccgtcac
     1441 ccaaaccgcg accggacctc gtgccgattc aaaggcagcc agcagcatca gcgccgggcc
     1501 aagggtcatg gccagataaa gcagcgatgg cggatatttt tcgcaattga tgaaagagag
     1561 gacggtgctc acggcattat cctgcggggt ccacggcacc ggatccccgt agaaattggt
     1621 ggcgcgcaac agcacgaaac cgatcgtgag cgccgcgccg agcatgaaaa gccgccgcct
     1681 gcgcccggtg cgtccctgca ggaataccgg cccaagggcg tagcctgccg ccatcacgcc
     1741 gatccacggg atcagcggat agagcacgaa gaacttgacg ccgggcacga tgtcaaaaag
     1801 cccaggctga tgcaggacat tccacagcgg cgcggcggcg ccgaactgcg tggccgtgat
     1861 gccgtcgaac gcattatggc ccacgatcat cgcgagtccg atcgcggcaa cgaccttgcg
     1921 cggcaggtgg acgaaggcgg cgagcgcaat catcgaaacg ccgatcgcaa aaatcacctg
     1981 cgcgacgaaa taattgaaat ggaggtcgaa actccagccg agacgcacga cagtgaactc
     2041 gatcagcacc agccagatgc cgcgcgtcag caggaagcgg ctgacgtcgc ctgtgctgcg
     2101 gcctttcacg ccgtagagat aggcggaaat cccggccaga aagatgaaag tcggcgcgca
     2161 gaaatgcgtc acccagcgtg tcaggaacag cgccggctcg gccacgtcgc gcggattgaa
     2221 gccgccattg
//
LOCUS       DASSRL010000456         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1639204, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000456 DASSRL010000000
VERSION     DASSRL010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639204"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1010)
                     /locus_tag="VFL49_09275"
     CDS             complement(369..1010)
                     /locus_tag="VFL49_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912212.1"
                     /translation="MKVSVVAAAWIAVAACGVGSSAQAGTIIPYPHPGTPNSEIYTFT
                     AAATGVITAFFAGSDAGHTDSIGMLVNGVPTGIVGLNNHTSSLGDSIVLGNVNVGDVL
                     TFFLVDANTASTWYSDANLNVDGKTQHAYSAAYDGLHPPLGVGIPAGTYIGFEDLSRA
                     QGGDFDYNDDSFVFTNVSTGETPLPAALPLFATGFGGLVLLAQRRNKRRRAAA"
     gene            complement(1199..2299)
                     /locus_tag="VFL49_09280"
     CDS             complement(1199..2299)
                     /locus_tag="VFL49_09280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET7912213.1"
                     /translation="MPRITVGVPVYKGADLLPKALECLRGQTFRDFEAIISVDGNDSE
                     TAAVCRPFLADSRFRMVVHSDRLDWVGNFNWLLQQDLQEFFCYRQHDDTTAPEFFEAL
                     IRVADKEPGAAAIYCDCKYSGGRSDVEAALSIKGDSPLDRMFQYVARLPNLMPTPVRG
                     LIRSAAIRQAGLVRADEFRAALQLLGWLANVLRWGDFTRVPQPLYYRLDHARSYTREF
                     WTGAYKNAWTTIFTGLLDAAMRVCRTPAERQFFQQLVLDRVVAYPGFQRNDDAHFAHE
                     LGKACLMRLEQEGRPDLLDGMLAALDQLQERVAVLRSIERTRVGRAVSQARQNYRFAR
                     VAYVGSPGRRIDYQIRHLAELAGKAARLLGNS"
     gene            complement(2316..>3338)
                     /locus_tag="VFL49_09285"
     CDS             complement(2316..>3338)
                     /locus_tag="VFL49_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912214.1"
                     /translation="GALSFAPFMEQHLGRPADSFLVSVPAVYIVHRDSQHSAEDVCAY
                     LDTVHRLVNEAAAGRERAYFGKDLGAPLKFWSDDEIEAEFDPSIAVAAVASPEIAINP
                     VALADNLRERVAQDPLIEVQCGRTVVGADLGPDGGFVATVAQGGSSRDRFDHIVNALW
                     DGRFALNEALGFRANRPWMHRLKYGVSFQLPRGARTPPSATFVLGPFGEVVTYGDGTI
                     YLTWYPECLRAISTDITPPDWTTYPAEPLRSIILHGTLRALSEVVHSLSDLDPQNLPE
                     AIVKGGAIVAWGKTDIYDPASELHRRFEIGVTTEGRFHSIDPGKLTMAPYFAKICADR
                     ISSGLN"
BASE COUNT          729 a          991 c          957 g          661 t
ORIGIN      
        1 cagaaaccga ttgtgacggg gtgggcaccg attaacgtat ggcatcgcgc tcgtattgtc
       61 gggtgattgc gcatgctcag cttgcttgca aaaaatcgag atgctctccg agtgttgacc
      121 gcggaacgtc gtgttccgtc atttcaggtc tggcacatat ggaccgtcac gatgccaaga
      181 cgatcgataa ccggccgttt ggtctggggg accgtatggc gccgacgcga cgacggccgc
      241 tggatttaca agcgccatgt cgagagcgcg gaagccactg aaaaacgcag ggccgttcga
      301 acgctcgtct cgaaaagcaa aacggccccc tttcggcggc cgctctagag attgcgcttt
      361 aaccgaggct acgccgccgc acggcggcgc ttgttccttc tctgcgcaag caaaacaagg
      421 ccgccgaaac cggtcgcgaa aagcggaagc gcagcgggca gtggcgtctc gcctgtcgaa
      481 acgttggtga agacaaacga gtcgtcgttg tagtcaaaat ctccgccctg cgcgcgtgac
      541 agatcctcaa acccgatata tgtgccagcc ggaattccaa cgccgagcgg cggatgaagg
      601 ccatcgtacg ccgcggaata ggcatgctgc gtcttgccat caacgttcaa attggcgtcg
      661 gaataccatg tactggccgt attggcgtcg acgaggaaaa acgtcagcac atcgccgaca
      721 ttgacattgc cgagcacgat gctgtcgccg aggctcgacg tatgattgtt cagtccgacg
      781 attccggtgg ggacaccatt gaccagcatg ccgatcgagt cggtgtggcc tgcgtccgat
      841 cctgcaaaga aagccgtgat aacgccggtg gctgccgcgg tgaacgtata gatctcggaa
      901 tttggtgtcc caggatgggg atagggaatt atcgtcccgg cttgcgcgct cgaaccaacc
      961 ccacatgccg cgactgcaat ccacgccgcg gccaccaccg acactttcat tgtactgacc
     1021 ttgctaaaac accacggagt aattttacga ttcatttcac agcgcgatca agtcgcttga
     1081 tctaaaattt tcgtactgtg tcccaatatc ggacaagtgc gactatcgta gaggtggcgg
     1141 gataacatct gctgaaaatc catccggatt tggataccac cggaccgcaa gcgatccgtc
     1201 agctgtttcc gagaagccgc gccgccttgc ctgccagttc cgcaagatgg cgaatttgat
     1261 aatcgattct gcgcccgggc gacccgacgt aggcaacacg cgcgaatcga taattttgcc
     1321 gcgcctggga aacagctctt ccgacccgcg tgcgctcgat tgacctgagc acagcgaccc
     1381 tttcctgcaa ttgatcgagg gccgcaagca tcccgtcgag taggtcgggc ctgccttcct
     1441 gctcgaggcg catcagacac gccttaccaa gctcgtgtgc aaagtgagca tcatcgttgc
     1501 gctgaaatcc cggataggca accactcggt caagcaccaa ttgctgaaaa aactgccgct
     1561 ccgcgggggt gcgacaaacg cgcatggcgg cgtcgagcaa tccggtaaat attgtcgtcc
     1621 acgcattctt gtatgcgcct gtccagaatt cgcgggtata actgcgcgcg tgatcgaggc
     1681 ggtagtacag cggctggggt acccgcgtga agtcgcccca ccgcaggaca ttggcaagcc
     1741 aacccagcaa ctgcagggcg gcccgaaatt catccgctct gacgaggccg gcttgtcgga
     1801 tcgcggcact gcgtatcagt cctctgacag gggttggcat cagatttggc aggcgcgcga
     1861 catactggaa cattcgatca agtggtgaat cgcccttgat cgaaagggcc gcttcgacgt
     1921 cactgcgccc tccactgtat ttgcaatcac agtagatcgc cgcggcgcca ggttctttgt
     1981 ccgcgacacg tatgagcgct tcgaaaaact ccggcgccgt tgtgtcgtcg tgctgccggt
     2041 agcagaaaaa ctcctgcaga tcctgttgca ggagccagtt gaaatttccg acccagtcga
     2101 gtcggtcgga atgcacgacc atccggaatc gtgagtcggc caaaaatgga cggcaaacgg
     2161 ccgccgtttc cgaatcattc ccgtcaacgg aaatgatggc ctcgaaatcc ctgaaggtct
     2221 ggcctcgcag gcattccagc gcctttggca ggaggtcggc gcctttgtat acgggcactc
     2281 ccaccgtgat gcgtggcaca gacctgcgaa ctcctttagt tcagacctga actgatccgg
     2341 tccgcgcaga ttttggcaaa gtatggcgcc atggtgagct ttcccggatc gatggaatgg
     2401 aagcggcctt ccgtcgtgac cccgatctca aatcggcggt gcagctcgct tgccggatca
     2461 tagatatccg tcttgcccca ggccacgatc gcaccgccct tgacgatcgc ttcaggcaga
     2521 ttttgagggt ccagatcgga aagagaatga actacctcag agagcgcgcg aagcgtccca
     2581 tgcaggatga tggaacgaag cggttctgcc ggataggtcg tccaatccgg cggcgtaatg
     2641 tccgtgctga tagcccgcag gcattccgga taccaggtga ggtagatcgt cccgtcaccg
     2701 taggtcacca cttcaccgaa ggggccaagc acaaaggttg cgctcggagg cgtccgcgcg
     2761 cctcgcggca gctgaaagct cactccgtat ttcagccggt gcatccaggg acggttggcg
     2821 cgaaatccca gcgcctcgtt gagcgcaaag cggccgtccc acaaggcgtt cacaatgtga
     2881 tcaaaacgat cacgcgacga tccaccctgg gctacagttg ctacaaaacc gccgtcgggg
     2941 ccgagatccg ctccgaccac cgtacggccg cactgcacct cgataagagg atcctgcgcg
     3001 acgcgctctc gtaagttatc tgccagcgcc accgggttga tggcaatctc cggacttgcc
     3061 acggcagcaa cggcaatcga agggtcgaat tcggcctcta tttcgtcgtc cgaccagaat
     3121 ttcagcggtg cgcccaaatc ctttccgaag tacgcccttt ccctgcctgc cgcggcttcg
     3181 ttgaccaatc gatgaacagt gtcgagatag gcgcagacat cctcagcgct gtgctggctg
     3241 tcgcggtgaa cgatgtacac tgcaggaacg gacaccagaa atgagtccgc tggtcgtccg
     3301 agatgctgct ccataaatgg ggcgaacgaa agcgcccc
//
LOCUS       DASSRL010000457         7959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_991846, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000457 DASSRL010000000
VERSION     DASSRL010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991846"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFL49_09290"
     CDS             <1..202
                     /locus_tag="VFL49_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7912215.1"
                     /translation="IPGLYACGNDMASVMAGSYPGPGTTLGPAIVFACRAAMHAKDAR
                     VPERIEPVDEQSLDRVARDCPL"
     gene            213..980
                     /locus_tag="VFL49_09295"
     CDS             213..980
                     /locus_tag="VFL49_09295"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7912216.1"
                     /translation="MGRLAGKVAFITGGGGGIGRATAERFAEEGAKVVIAEIDVGRGE
                     AAAQSARSRASNSGGDAHFIHCDIRERTNVEAAFAETIKRFGGLHILHNNAGGSSPED
                     GRVTDVSEDEFWRVIRLDVYGTFLCSKLGIPYMIKSGGGSIINMSSIVALRALPGRDC
                     YTSAKGAVAALTRSMAVEYAADKIRVNAIAPGVVLTERVKRLLEGSKDIEKLAATHLL
                     GLGLPNHMADTAVYLASDESAITTGQVISVDSGATIR"
     gene            1040..2815
                     /gene="argS"
                     /locus_tag="VFL49_09300"
     CDS             1040..2815
                     /gene="argS"
                     /locus_tag="VFL49_09300"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7912217.1"
                     /translation="MSSQAKPQHLFAAMLAAVHAATVAVLGSDADVSRIVVEPPRDPT
                     HGDMATNAAMVLAKDAGRKPRDLAESIAAKLRSDGKVASVDIAGPGFINLTLKPNVWA
                     EELRLVLEAGRDYGRSDMGKSEKVNVEYVSANPTGPMHVGHGRGAVFGDALANLLAFV
                     GYKVTREYYINDAGAQVDVLARSAYLRYREALGETITIPDGLYPGEYLRAVGKDLANA
                     HGPALKDKPEAEWLPIVRRRAIDMMMAEIRNDLQALGIVQDEFFSERSLIAKGADRVA
                     ETIDWLKREGHVYEGRLPPPKSGAVEDWEDREQTLFRSTAFGDDVDRPLKKSDGSYTY
                     FATDIAYHKSKLDRGFRHLIDVWGADHGGYVKRMQAAVKALSGAEADLDVKLVQLVRL
                     LRAGEPVKMSKRAGEFITLREVVDEVGRDAVRFDMLYRKNDAVLDFDLAKVIEHSREN
                     PVFYVQYGHARGQSVFRNAREEIPDLPQSASERAAMLRRAPLEKLTDAGEVSLLRRIA
                     LFPRLVEAAAGAHEPHRVAFYLYELASEFHAHWTKGKDLPHLRFIIQNDRQTTAARLA
                     LVEGIVAILASGLALLGVSAPDEMR"
     gene            2891..4318
                     /locus_tag="VFL49_09305"
     CDS             2891..4318
                     /locus_tag="VFL49_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7912218.1"
                     /translation="MADDQGNRPYRASEPPPARGAGKTSGSDPLAELARLIGQTDPFG
                     EFGRDVARRSPPSQSVARADWNQPLGTSYTAQYGGDARVPEPSPRYAGSSPYAPPSSA
                     ASTTGPQDYDRLNYERQPYEEASAGGADHYVAEQATDRVPLGQSDYQQDFYDEAPVED
                     EYYEDAKPSRRRMAIAAIAGVVALAVIGTAGAFGYRALFGSPGSTKPPPVIKADTAPS
                     KIVPATAGKDAQSGKLITDRVNERGQSEKLVSREEQPLDRPTAVALPQSAPQAALGNG
                     VVASEPKKVRTIAIHPDQPVGADAGASSMSAAAAAAAKPMTAAPAPRVAAAPTRPAPD
                     TTADAEESAPTRSAQPRPAPSASGSAPLSLNPNAAPSRAAAPARTASVAPQASASSTG
                     GPGSYVQVSSQRSESEAQAAFRGLQAKFPNQLGGRQPYIHKVDLGAKGTYYRAMVGPF
                     ASAGEASQLCSSLKAAGGQCLVQRN"
     gene            4366..5406
                     /gene="nagZ"
                     /locus_tag="VFL49_09310"
     CDS             4366..5406
                     /gene="nagZ"
                     /locus_tag="VFL49_09310"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET7912219.1"
                     /translation="MAARAFITGLEGLTLSANERAFLRDSRPWGLIIFKRNVSTPDQV
                     TELIQSFRDIAGSETPVLVDQEGGRVQRLGPPHWPAYPPGARYGVLYEEEPAKGIAAA
                     RLAGHLIAADLRPLGIDIDCLPVADVPVASGDPVIGDRAYGTDPGKVARIAKAIADGL
                     QAGGVLPVLKHLPGHGRATADSHQRLPVVDTDRAMLEATDFAAFRPLASLPLGMTAHV
                     VFSAIDPVAPATTSVTMVRQVIRGFIGFQGLLMSDDISMNALSGSVAERSRAALSAGC
                     DIVLHCNGDLKEMIAVANEAPELSGEAAKRAHAALAARKAPEEFDDEAARKLFTQMIA
                     DKRAEPQRKTGS"
     gene            5403..6224
                     /locus_tag="VFL49_09315"
     CDS             5403..6224
                     /locus_tag="VFL49_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HET7912220.1"
                     /translation="MSSEDFDTTLAADRGTTEPAMIVDVEGFEGPLDLLLMLARQQKV
                     DLSKISILALADQYLSFIEAARQLRLELAADYLVMAAWLAYLKSRLLLPETEPGDGPS
                     AEDMALALANRLRRLEAIRDVATRMMERPQLNRDVFARGLPEPIEEIKQPEWSATLYD
                     LLSAYATQRSRRALSRVRFKQRTVWSLADARNALERLIGQSADWSRIDQFLISYVVEP
                     ALAATVFASSFASALELVREGVAEIHQKESFAPIYMRKRVGAIGIATTHDAQENS"
     gene            6238..6960
                     /gene="scpB"
                     /locus_tag="VFL49_09320"
     CDS             6238..6960
                     /gene="scpB"
                     /locus_tag="VFL49_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET7912221.1"
                     /translation="MPSVAKKLVVVQSNEPAPEVAPTESTRPEELRLLEALLFAAGQP
                     LDEAMLARRLPNGVNVKTALAQLKDEYATRGVNLVRVGKKWTFRTATDLSWLLTKQIV
                     ETRKLSRAAIETLAIVAYHQPVTRAEIEEIRGVVTAAGTLDVLLKTGWIRPRGRRKAP
                     GRPITYGTTEEFLSHFGLEEVGDLPGLDELKGAGLLEGNLPPGFGVPLPSDDSTLRDD
                     EDPLDPGDLDLGLAPPASRSEK"
     gene            7116..7361
                     /locus_tag="VFL49_09325"
     CDS             7116..7361
                     /locus_tag="VFL49_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7912222.1"
                     /translation="MGSLSIWHWIIVLLVVMVLFGGRGKISSLMGDFAQGIKAFKKGM
                     SEDEKTADAKSEPVKTIEASGSATKSEAERRAEGVRS"
     gene            7448..7903
                     /gene="tatB"
                     /locus_tag="VFL49_09330"
     CDS             7448..7903
                     /gene="tatB"
                     /locus_tag="VFL49_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HET7912223.1"
                     /translation="MFDIGWGELVVIGIVALIAIGPKELPTVLRTLGQYMGKVRRMAS
                     EFQGQFQDAMREAELAELKKQAEDLKSSVSDLGNFDPLADTQKQIESAFDDAAKPELP
                     KPDSASAAEAPEAAAPAASDVALPEPAAPVGEKDFAAAEAPPKQAGGNA"
BASE COUNT         1567 a         2347 c         2583 g         1462 t
ORIGIN      
        1 cattccgggc ctctacgcct gcggcaacga catggcctcg gtgatggcgg gaagctatcc
       61 aggtcccggc acgacgcttg gtccggcaat cgttttcgcc tgccgcgcgg cgatgcatgc
      121 caaagacgca cgggttccgg agcgtattga gccggtcgac gagcaaagcc tcgatcgcgt
      181 ggcgcgcgac tgcccgcttt aaggagagca ttatgggacg ccttgcaggt aaagtggcat
      241 tcatcacggg cggtggcggc ggaattggcc gtgcgacggc ggagcggttt gcggaagaag
      301 gggccaaggt cgtcatcgcc gagatcgatg tcgggcgtgg cgaagcggcg gcgcaatccg
      361 cacgctcgcg cgcgtcgaat tcgggcggcg atgcccactt catccattgc gatatccgcg
      421 aacgcacaaa tgtcgaagca gcctttgccg aaaccatcaa gcggtttggc ggactccaca
      481 tccttcataa caatgccgga ggctcgtcgc cggaagacgg ccgcgtgacg gatgtgtccg
      541 aggacgaatt ttggcgcgtc atcagactcg atgtctacgg gacgttcctt tgctcgaaac
      601 tcggcattcc gtacatgatc aaatcgggtg gcggctctat catcaatatg tcgtcaatcg
      661 tcgcgctgcg cgcgctgccg ggccgggatt gctatacctc ggcgaaaggc gcagtcgccg
      721 cgttgacgcg atcgatggcg gtggaatatg ccgccgataa gatcagggtg aacgcgatcg
      781 cacccggcgt ggtgcttacc gagcgcgtca agagattgct cgaaggatca aaggacatcg
      841 agaagctggc agcaacgcat ctgcttggcc tcggcttgcc aaatcatatg gccgatacgg
      901 ccgtttatct cgcttcggac gagtcggcga tcacgaccgg tcaggtcatt tcggtcgata
      961 gcggcgcgac aatcagatag ctctcgatgg cgattgcgaa ttttgacttg ctttgcgaat
     1021 tgcgttagcc gcagccctca tgtcgagcca ggcaaaaccc cagcacctgt ttgcggcaat
     1081 gctcgccgcc gtgcacgccg caacggtggc tgtgctcggc tccgacgcgg atgtttcgcg
     1141 gattgttgtc gaaccgccgc gcgacccgac gcatggcgat atggcgacaa acgccgcgat
     1201 ggtgctggcg aaagacgccg gcaggaaacc gcgcgatctt gctgaaagca ttgccgccaa
     1261 gctgcgcagt gacgggaagg tggcgtcggt ggatatcgcg gggcccggtt tcatcaatct
     1321 cacgctcaag ccgaatgtct gggctgagga actgcgcctc gtgctcgaag ccggacggga
     1381 ctatggccgc agcgacatgg gcaaaagcga gaaggtgaat gtcgaatatg tctcagccaa
     1441 tcccaccgga ccgatgcatg tcggccacgg ccgcggtgcg gtgttcggcg acgcattggc
     1501 caacctgctc gcttttgtcg gctataaagt cacgcgcgag tactacatca atgatgcggg
     1561 cgcgcaggtc gatgtcctcg cgcggtcggc atatttgcgt tatcgcgagg ccctcgggga
     1621 aaccatcaca attcctgacg gtctttaccc cggcgagtat ctcagagccg tgggaaagga
     1681 tctcgccaac gctcatggcc ccgcgttgaa ggacaaaccg gaggcggagt ggcttccgat
     1741 cgtgcgcaga cgcgcgatcg acatgatgat ggccgagatc cgcaatgatc tgcaggcgct
     1801 ggggattgtc caggacgaat tcttctccga aaggtcattg atcgcgaagg gtgccgaccg
     1861 cgtcgccgag accatcgact ggctgaaacg agaagggcac gtctacgaag gacgacttcc
     1921 accgcccaag agcggtgcgg tagaggactg ggaagaccgc gagcaaaccc tgtttcggtc
     1981 gactgccttc ggcgacgacg tcgatcggcc actgaagaag tcggacggca gctatacata
     2041 tttcgcgacc gacattgcct atcacaagtc aaaactggac cgtggcttcc gccacctgat
     2101 cgatgtgtgg ggggccgacc acggcggcta cgtcaagcgc atgcaggcgg ctgtgaaggc
     2161 gctgagcgga gcagaggctg atctcgacgt aaaactcgtg caactggtgc gcctcctgcg
     2221 ggcgggcgag ccggtgaaaa tgtcgaaacg tgccggtgaa ttcattaccc tgcgtgaggt
     2281 ggtcgatgaa gtggggcgcg acgccgttcg cttcgacatg ctttaccgaa aaaacgatgc
     2341 ggtcctcgat ttcgacctgg caaaagtcat cgagcattca cgtgagaatc cggtcttcta
     2401 cgtgcagtac gggcacgcgc gcggacaatc ggtatttcga aatgcgcggg aggaaattcc
     2461 ggacttgccg caatccgcat cggagcgcgc ggcaatgctc aggcgggcgc cgctcgagaa
     2521 actgaccgac gcgggcgaag tttcattgtt gcgccgcatc gcgctctttc cgcgtctggt
     2581 agaggccgcc gcgggcgctc acgaacccca tcgggttgca ttctatctgt atgaattagc
     2641 aagcgaattt catgcacatt ggacgaaagg aaaagacttg cctcatttac gcttcattat
     2701 ccagaatgat cgacaaacga cagcagcccg gcttgcgttg gtggagggga tcgtcgcaat
     2761 tttagcctcg ggcctcgcat tgctcggggt cagcgcgccg gatgagatgc gataggcctc
     2821 gtccattggt ctgcgtccgc gtggattgta aaagaccatt acgaggatga gttgggtaac
     2881 cggggaacga atggcggacg atcaaggcaa cagaccgtat cgtgcaagtg aaccgccgcc
     2941 agcgcgcggc gcagggaaga cgtccggcag cgatccgctc gcagagctgg cgcgtctgat
     3001 cggacagacc gatccgttcg gagaattcgg ccgcgacgtc gcgcgccgtt cgcctccgtc
     3061 gcaatcagtc gcgcgggcgg attggaacca gccgctcggg acgtcctaca ctgcgcagta
     3121 tggcggcgat gcacgtgttc ccgaaccgtc accccgatat gcaggcagca gcccttatgc
     3181 gcctccctcg agtgccgcga gcacgactgg tccgcaagac tacgaccgcc tgaactatga
     3241 acggcaaccc tacgaagagg catctgccgg tggagccgat cattatgtag ccgagcaggc
     3301 aacggaccgg gttcctctcg gtcagtccga ctatcagcag gatttttacg acgaagctcc
     3361 ggttgaggac gaatattacg aagacgccaa accttcgcgc cgcaggatgg ccattgcggc
     3421 aatcgccggt gttgtcgctt tggcagtgat cggcaccgcg ggtgcatttg ggtatcgcgc
     3481 gctctttggc tcgcccgggt cgaccaagcc gccaccggtg atcaaggctg atacggcgcc
     3541 gagcaagatc gttccggcaa cagcaggtaa ggatgcccag tccggcaagc tgattactga
     3601 ccgcgtgaac gagcgcgggc agagcgaaaa gctggtgtcg cgcgaagagc agcctctcga
     3661 ccggccaacg gccgtcgccc tgccgcagtc ggcgccgcaa gcggctctcg gaaatggtgt
     3721 ggtcgccagc gagccgaaaa aagttcgtac gattgcaatt cacccggatc agccggtcgg
     3781 ggctgatgcc ggtgcgtcgt cgatgtcggc cgcggccgca gcagctgcaa agcccatgac
     3841 tgccgctccg gcgccgcgtg tcgcagccgc cccgacacgc ccggctccgg acaccactgc
     3901 agatgccgaa gagagtgcgc cgacgcggtc tgctcagccg cgcccggccc cctctgcgtc
     3961 gggcagcgcg ccgctctcgc tcaatccgaa tgctgcgcct tcgcgggcgg cggcaccggc
     4021 acggacggcc tcggtggcgc ctcaggcctc cgcgtcatcg acgggcgggc ccggtagcta
     4081 cgtgcaggtg tcctcgcagc gcagcgaaag tgaggctcag gcggctttcc gcggcctaca
     4141 agccaagttc cccaatcaac tgggtggacg ccagccctat atccacaagg tcgatctggg
     4201 cgccaaggga acttattacc gcgccatggt tgggcccttc gccagtgccg gcgaagccag
     4261 ccaattgtgc tccagcctca aggctgctgg cgggcagtgc ctcgtccaga ggaattaaca
     4321 ggcgcaaact tgacccctgc gacatgcgcg ggctaatccg cgccgatggc cgcgcgcgca
     4381 tttatcacgg gattggaggg acttacgtta tccgccaatg agcgggcctt tttgcgcgac
     4441 tcacggccat ggggtctcat catcttcaag aggaacgtaa gtactcctga tcaagtgact
     4501 gagcttattc agtcattccg cgatatcgcc ggcagcgaaa cgccggtgct tgtcgatcag
     4561 gagggcggac gggtgcagcg tctggggccg ccgcactggc ctgcttatcc gcccggcgcg
     4621 cgttacgggg tgctctacga ggaagagccg gcgaagggta ttgcagccgc gcggctcgcg
     4681 ggccatctga tcgccgcgga tttaagaccg ctgggcatcg acattgattg tcttccggtc
     4741 gcggatgttc cggttgcaag cggcgatcct gtgatcggag accgtgccta tggcaccgac
     4801 cccggcaaag tcgcccggat cgccaaggct attgccgacg gtctgcaggc cggaggtgtc
     4861 ttgcccgtgc tgaagcatct gcccgggcac gggcgtgcca ccgcggacag ccatcaaaga
     4921 ctgccggtcg tggataccga ccgcgcgatg ctcgaggcca ccgatttcgc ggcgttcaga
     4981 ccgctggcga gtctgccgct tggaatgacg gcacatgttg tgtttagcgc gattgacccc
     5041 gtcgcgccgg ccaccacttc ggtcacaatg gtgaggcaag tgattcgcgg ctttatcgga
     5101 ttccagggac ttctgatgag tgacgacatc tcgatgaacg cgctgtccgg cagcgttgca
     5161 gagcgcagcc gcgctgcgct gtcggccggg tgcgatatcg tgcttcattg caacggcgat
     5221 ctgaaagaaa tgattgcggt cgcaaacgag gcgcccgagc tgtcgggaga agcggcgaag
     5281 cgggctcatg ccgcgctcgc ggcgcgtaag gcgccggaag aatttgatga tgaagctgcg
     5341 cgcaagttgt tcacacaaat gatcgccgac aagcgggcag aacctcagcg gaagaccgga
     5401 tcatgagcag cgaggacttc gacacgacgc tcgcggccga ccgcggcacc actgagccgg
     5461 cgatgatcgt cgacgtggaa ggttttgagg gcccgcttga tttgttgttg atgctcgcgc
     5521 ggcagcaaaa agtggatcta tcgaaaattt cgatcctcgc tctggctgat cagtacctgt
     5581 cattcatcga ggccgcgcgt cagttgcggc tggaacttgc cgcagattac ctggtgatgg
     5641 cggcttggct cgcttacctc aagtcgcgcc tgcttctgcc ggaaaccgag ccgggggatg
     5701 ggccaagcgc ggaagatatg gcgctggcct tggccaaccg gctgcgccgg ctggaagcga
     5761 tccgcgatgt cgcgacccgg atgatggagc ggccgcaact aaatcgcgac gtttttgcgc
     5821 gcggcctgcc ggagccaatc gaggaaataa agcagcccga gtggtcggca acactctatg
     5881 acctgctttc ggcctatgcc acgcagcgct ctcgccgcgc attgtcccgc gtccgcttca
     5941 agcagcgtac ggtctggtcg ctcgccgacg cacgcaacgc acttgagcgt ctgatcgggc
     6001 agtccgcgga ctggtcgcgt atcgaccagt tcctgatcag ttatgtggtg gagcctgcgc
     6061 tcgcagcgac ggtgtttgcg tcttcgttcg cctccgcgct cgagcttgtg cgggagggtg
     6121 ttgcggaaat tcaccagaag gagtcattcg cgccgatcta tatgcgtaag cgcgtcggcg
     6181 cgattggaat agctacgacg catgacgcgc aagaaaactc ataaggggaa agagtccatg
     6241 ccgagcgtgg ccaagaagct cgtggtggtg caaagcaacg agccggcgcc tgaggttgcc
     6301 ccaacagagt caacgcggcc cgaggaattg cgactgctgg aagccttgct gtttgccgcc
     6361 ggccaaccgc tggatgaggc gatgctcgca cgccggctgc caaacggcgt gaacgtgaag
     6421 acggcgctcg cgcagctcaa agatgaatat gccacgcgcg gcgtcaatct ggtgcgcgtc
     6481 ggcaagaaat ggactttccg tacggccacc gatctgtcgt ggctgctgac gaagcagatc
     6541 gtggagaccc gcaagctttc gcgggcggca atcgaaacgc ttgcgatcgt cgcctatcat
     6601 cagccggtta cacgcgccga gatcgaggaa attcgtggcg tggtgactgc cgccggcacg
     6661 ctcgatgtgc ttctcaagac tgggtggatc cgcccgcgcg gccggcgcaa ggcgcccggg
     6721 cggccgatca cgtatgggac gacggaagaa ttcctttctc atttcggtct ggaggaggtc
     6781 ggagacctgc cgggcctcga cgaactcaag ggcgctggct tgctggaagg caacttgcct
     6841 ccgggtttcg gcgtgccgct cccttcggac gactcgacat tgcgcgacga cgaagatccg
     6901 ctcgatccgg gcgatctcga tctgggtctg gcgccgccgg cgtcccgctc agaaaagtga
     6961 atacgcgcca gttaaggtaa atggcgtaat ttcaagggat tgcgcgtcgc tttgaccgcc
     7021 agcgaagtcc ttgtttttgc cgccagcgga gcctagtttc acattgaaaa catggcggga
     7081 cgagccgaaa cgccgtcgcc gcggaggatt tgccaatggg ttccctaagt atatggcact
     7141 ggatcatcgt cctgctggtc gtgatggtcc tgtttggagg ccgtggaaag atttcatcgc
     7201 tcatgggtga ttttgcccag ggcatcaagg ccttcaagaa gggcatgtcc gaggacgaaa
     7261 aaaccgcgga cgctaaaagt gaaccggtca agacgatcga agccagcggc agcgcgacga
     7321 aatcggaagc cgagcggcgc gccgagggcg tgcgctcctg atcgctgcgc cggtccgcgc
     7381 gggggaagcg gccggctcgg ttgtcgcagt ggcgccgctg cgtcggcgcc tcggagttga
     7441 ctaatccatg ttcgacattg gttggggcga gctcgtcgtc atcggcatcg tcgcgctgat
     7501 cgcgatcggg ccgaaggagc tgccgactgt tttgcgtacg cttggccagt atatgggcaa
     7561 ggtgcgtcgc atggcgtccg aatttcaggg tcagtttcag gacgccatgc gcgaagccga
     7621 gttggccgag ctgaaaaaac aggctgaaga tctaaaaagc tcagtcagcg atctcggcaa
     7681 cttcgacccg ctggccgaca cgcaaaaaca gatcgaaagc gcattcgacg acgcggccaa
     7741 gcctgaattg ccgaagcccg actcggcgag tgccgcagag gcgcccgaag cggccgcacc
     7801 tgcggccagc gacgtcgcgt tgcctgagcc cgctgctccg gtcggtgaaa aagactttgc
     7861 ggccgctgaa gcgccaccca agcaggctgg gggcaacgca tgacccacga ggacatcgag
     7921 gccacaaagg ccccgctgat ggatcacctg atcgagctg
//
LOCUS       DASSRL010000458         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_816961, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000458 DASSRL010000000
VERSION     DASSRL010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816961"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1066)
                     /locus_tag="VFL49_09335"
     CDS             complement(299..1066)
                     /locus_tag="VFL49_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET7912224.1"
                     /translation="MSSSISTREELLSSARRASGRCWRAVEAQNLISTAKLTDTAAEQ
                     RVLEERIEKSKPKIPSECRHLNFLLSTPFRYGAPYPRGSRFRKAGHTPGVFYGAENPD
                     TAIAELCFSRLVFYSESPATRWPPEAGEYTAFAVRYATERAIDLTHPPLNSREAAWTR
                     KMRYNECQELADLARSVEINVIKYASVRDPKAQANIALLTCRAFADTDPVERQTWHII
                     LSSHGARALCEMPRVSLDFDRNAFRGDPRIDRMKWER"
     gene            complement(1026..1412)
                     /locus_tag="VFL49_09340"
     CDS             complement(1026..1412)
                     /locus_tag="VFL49_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HET7912225.1"
                     /translation="MSVKTRRRAEPLPDESRVVTKATLRAAERLRIKNAVLARIIGLS
                     EPTISRMYKGTYVLPPESKNFELAVLFIRLYRSLDGIIGGDDSIAADWLKNSNTVLDG
                     APLELIQSVSGLTNVIEYLDSRRAVV"
     gene            1694..>2782
                     /locus_tag="VFL49_09345"
     CDS             1694..>2782
                     /locus_tag="VFL49_09345"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7912226.1"
                     /translation="MMTEHMLDPLTEWLTKAGLRGEPEDALVSGFCKRAVDAGLPVSR
                     ALILVDTLHPVYEGRVIRWGHDPSQPVVHEYGRTALPDDAADLHPSAIEQATHVTRWY
                     ASPFFHMLQTGESLLRRRVTAESEAEFPVLRDYRADGMTDYVAIINRFSPEGTIGEMD
                     CVYSSWVTTRAGGFTDADIAALAQLAPRLALAVKAAALARMTATLMETYLGRDAGRRV
                     LSGRIMRGVADRIDAVIWFSDLRGYTRITDSAPEQVIPLLNDYADVIISAIHAHGGDV
                     LKLIGDGILAIFAAEDRERACTAALAAAVAARSDVAKLNERRARDGLPVTDMYLGLHV
                     GEVFYGNVGSMERLDFTVVGPAVNETSRI"
BASE COUNT          573 a          791 c          820 g          598 t
ORIGIN      
        1 cgcggcatgt cgcccgaaga tttgcacaac cgcattgcaa gcgatgtggc caaatgggcg
       61 caagtgatcg agacaacagg aattaagaag cgttgactgc ggctggatga ttccgccgcg
      121 gattctatcc gttccatccg cattcaaaac ggtcaaccgg ccgtgacgac ttgtcgctga
      181 tctcgcgcgc cgacgaagtc atcgagtgaa ttcggaatgt tagggacatt tctcggtgac
      241 attgtccgat cgttttgatc cccgcttcga tgaaaactat ttcaacctaa aacaccagct
      301 agcgttccca cttcatgcgg tcgatgcgcg gatcgccacg gaaagcattt cgatcaaaat
      361 ccaggcttac gcggggcatt tcgcacagcg cccttgcgcc atgactgctc aggatgatgt
      421 gccaggtttg gcgctccaca ggatcggtat cggcaaaggc gcgacaggtc aggagcgcaa
      481 tattcgcctg agcttttgga tccctcacgg acgcatattt aatgacgttg atctcaactg
      541 accgcgcgag atcggcaagt tcctggcact cgttatagcg catcttgcga gtccacgcgg
      601 cttcccggct gttgagcggc ggatgggtca ggtcaatcgc acgctcagtc gcataacgaa
      661 cggcaaatgc cgtgtattcg ccggcctctg gcggccacct ggtggccgga gattcggagt
      721 aaaataccag ccgggaaaag cacaattcgg cgatcgccgt gtcgggattt tcggccccgt
      781 aaaacacgcc cggcgtatga ccggcttttc taaaacggga gccgcgcgga tatggagcgc
      841 cgtaccgaaa tggagtggaa agcaaaaaat tcagatgccg gcattcggac ggtatctttg
      901 gcttggattt ttcgatgcgc tcttcgagaa cgcgttgctc cgcagcagtg tccgtaagct
      961 tggcagttga tatcagattt tgtgcttcga cagcccgcca gcatcgtccc gacgctcgac
     1021 gcgcgctaga caacagctct tcgcgagtcg agatactcga tgacattcgt caatccggaa
     1081 acagattgga ttaattcgag cggagcaccg tcgagaacgg tgttgctgtt tttgagccaa
     1141 tcggcagcga tggaatcatc gcccccgata attccatcga gcgaacgata aagccgtatg
     1201 aaaagaacgg cgagctcaaa gttcttgctc tccggcggca gcacgtaagt gcctttgtac
     1261 atgcgcgaaa tggtcggctc cgacagcccg ataattcttg cgagcacggc attcttgatt
     1321 cttagccgtt cggcggcgcg aagagtcgcc tttgtcacga ctcttgactc gtcgggaaga
     1381 ggttcggcgc gccgcctcgt tttgactgac atggctccct ccgggatcgc atagaaatag
     1441 tatcactatt ttatttctat ggaaagatac ttttcgttcg ttagaacgat ataaagctga
     1501 tatttctaag gaaataatta cgaaaccagg ccccgacaag gggtccctgg gcttaccggc
     1561 atcgcttgtc gggcagctct cgacgatttt ccaaaagcgg ggaagattta cgtgccggga
     1621 ggcgccctgg cgatagttta tttggcaggc cgcgatcgaa acgtttcgat taaaaggatc
     1681 gcgatgagtg gcaatgatga ctgagcacat gcttgatcct ttgactgaat ggctcaccaa
     1741 agccggcctg cgcggcgagc cggaagatgc gctggtgagc ggcttctgca agcgcgccgt
     1801 cgacgccggc ctgccagtct cgcgtgcgct gattctcgtt gacacgcttc acccggtgta
     1861 cgaaggccgt gtaatccgtt gggggcacga tccgtcgcag cccgtcgtgc acgaatacgg
     1921 ccgcacagct ctgccagacg atgccgcaga tttgcacccg tccgccatcg agcaggcgac
     1981 gcacgtcacg cgctggtatg ccagcccgtt tttccacatg ttgcagaccg gggagagctt
     2041 gctgcggcgc cgggtcacgg cggaaagcga agccgagttt ccggtgctcc gggactacag
     2101 ggccgatggc atgaccgact atgttgccat catcaatcgc ttttcccctg aaggcaccat
     2161 cggcgaaatg gactgcgtct actcttcgtg ggtgacgacg cgcgccggtg gcttcaccga
     2221 tgccgacatt gcagcgctcg cgcagcttgc tccgagactt gcactggcgg tcaaggcggc
     2281 cgcgctcgca cgcatgaccg cgaccttgat ggagacttat ctcggccgcg atgcgggacg
     2341 tcgtgtgctc agcggccgca tcatgcgtgg ggtcgccgat cgcatcgacg ccgtcatctg
     2401 gttcagcgac ctgcgcggtt atacgcgcat caccgattcg gcgcccgagc aggtcattcc
     2461 gctgctgaac gattacgccg acgtcattat ctcggcgatc catgcgcatg gcggagacgt
     2521 gctgaagctc atcggcgacg gtatcctggc tatctttgct gccgaagatc gcgagcgtgc
     2581 ctgcacggca gcacttgccg ccgcggtggc ggcccgcagc gatgtggcaa agctcaacga
     2641 acggcgcgct cgcgacgggt tgccggtcac cgacatgtat ctcggtctgc acgtcggcga
     2701 ggtgttctac ggcaatgttg gcagcatgga gcgcctcgat ttcaccgtgg ttgggccggc
     2761 cgtcaatgag accagccgaa tc
//
LOCUS       DASSRL010000459         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_694792, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000459 DASSRL010000000
VERSION     DASSRL010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694792"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFL49_09350"
     CDS             complement(<1..536)
                     /locus_tag="VFL49_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912227.1"
                     /translation="MAVGVLPATEIPSEASARTRSRQRAVRQFKLSLLLPLIALFLFL
                     LTPVLLLQLYFSFHQWTVYLSSWWDAEFVGLDLFQEVLTDPRFGWAVVRSLAFATGST
                     LGCFVFGFLLAYLMYKPFRGQAFYYIIFILPMLTVPVVVAYTAEMMLYQSGPINDIIS
                     KIIGVDFKPAWLTNPHIAL"
     gene            complement(599..2041)
                     /locus_tag="VFL49_09355"
     CDS             complement(599..2041)
                     /locus_tag="VFL49_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7912228.1"
                     /translation="MSRSGFSRRTFLQGSVGLTVANFVPGTAPFAHAATMEEQTIAAA
                     KTVGKADVNGMIWSPYLVPMKPVIAEFTKETGISLGAVQDISIFDAPQRAMAEALSRS
                     PQFDFIHIDSNMIPSLASAGYLEPLDEYIKKAGFHLDAVGDYGNFMKYGGQTYGVPTD
                     GNVHIQYTRKDLLDNPDNKKRFADKFGVELKVPETWEDNLRLQEFLMDPSKDLYGSGN
                     LRNRANGVTWWYMIFYSAGGFPFDDDMKPTLDTPAGQYAVDLYLQDKKVNHPEAANWG
                     TPQMIPRIAQGHVVSCQYWDGTCKTIANPKQSKTVGQFYFSLVPGSKFTGKMVHRSIS
                     SPLAAILINKYSPRKAQAAYMALWLGSGKNSIPIVSDPVNAFNDAWAKEHMKAEPVLK
                     AYTPEGIKAVETNFQIVAPPIYLTGYLEFQDALGKNLSEAYVGQLPAKDVLKKTENEW
                     NAIIGRIGRTKLKKDLASYKAVMPTRAVPA"
BASE COUNT          509 a          715 c          740 g          525 t
ORIGIN      
        1 agcgcgatat gcggattggt cagccacgcc ggcttgaaat cgacgccgat gatcttcgag
       61 atgatgtcgt tgatgggacc gctctggtac agcatcatct cggccgtgta ggcgacgacg
      121 accggtacag tgagcatcgg caatatgaat atgatgtagt agaaggcctg cccgcgaaac
      181 ggcttgtaca tcagataggc gagcaaaaag ccgaatacga agcagcccag cgtcgaaccg
      241 gtcgcgaaag caagggaccg tacgacagcc cagccgaatc tcggatcggt cagcacttcc
      301 tggaacagat cgaggccgac aaactcggca tcccaccagg agctgagata gaccgtccac
      361 tggtggaacg aaaaataaag ctgcagcaaa aggaccggcg tcagcaaaaa caaaaacagc
      421 gcgatcagcg gcagcagaag cgaaagcttg aactgccgga ccgcgcgctg gcgcgaccgg
      481 gtgcgtgccg aagcttcgga cgggatttct gtggctggaa gtacgccgac ggccatgctc
      541 tggctcttcc ttgcaacatg tcgggcgggc ccgtcggacc cgcccggctt atgccccgtc
      601 aggccgggac cgcgcgtgtc ggcatcacag ccttgtagct tgcgagatcc ttcttgagct
      661 tggtacggcc gatgcgtccg atgatggcgt tccactcgtt ctcggtcttc ttcagaacgt
      721 ccttcgccgg caattggccg acataggctt ccgaaaggtt cttgcccagc gcgtcctgga
      781 attcgagata gccggtgagg tagatcggcg gcgcaacgat ctggaaattg gtctcgaccg
      841 ccttgatgcc ttcgggtgta taggctttca gcaccggctc cgccttcatg tgctccttgg
      901 cccaggcgtc attgaatgca ttgaccggat ccgaaacgat cggaatgctg tttttgcctg
      961 agccgagcca cagcgccatg taggcggctt gcgctttccg cggcgagtac ttgttgatca
     1021 ggatcgccgc gagcggcgat gaaatcgacc ggtgcaccat cttgccggta aatttcgagc
     1081 ccggtaccag gctgaaataa aattgaccca cagtcttcga ctgtttcggg ttggcgatcg
     1141 tcttgcaggt cccgtcccag tattggcagc tgaccacgtg cccctgcgca atccgcggaa
     1201 tcatctgcgg cgtgccccag ttggccgcct ccggatggtt caccttcttg tcctggagat
     1261 agaggtccac agcgtattgg ccggccggcg tgtcgagcgt cggcttcatg tcgtcgtcga
     1321 aggggaagcc gccggcgcta tagaaaatca tgtaccacca ggtcacgccg ttcgcgcgat
     1381 tgcgcagatt gcctgacccg taaaggtcct tcgatggatc catgaggaat tcctgcaggc
     1441 gcaggttgtc ttcccatgtc tcaggcactt tcaactcgac gccgaatttg tcggcgaagc
     1501 gcttcttgtt gtcaggattg tcgagcaaat ccttgcgcgt gtactgaatg tgcacgttgc
     1561 catcggtcgg aacaccgtag gtctggcctc catacttcat gaagttgccg tagtcgccga
     1621 ccgcgtcgag atgaaagccg gctttcttga tgtattcatc cagcggttcg agatatccgg
     1681 ccgatgcaag ggacgggatc atgttagagt cgatatggat gaaatcgaac tgcggggagc
     1741 gcgacagcgc ttcggccatc gcccgctgcg gtgcgtcgaa aatggagatg tcctgcaccg
     1801 cgccgaggct gatccctgtt tccttggtga actcggcaat gaccggcttc atcggcacca
     1861 ggtaaggcga ccagatcatt ccgttgacgt ctgccttgcc gaccgtcttc gcggccgcga
     1921 tggtttgctc ctccatcgtc gcggcatggg cgaacggcgc cgttcccggc acgaaattcg
     1981 ccacggtaag tccgacactg ccctgcagga acgtacgtcg cgaaaaaccc gatctggaca
     2041 tgacttcctc ctcgcctgtg ttggctttgt gcgtttattt gtggggtgac ccggcggagc
     2101 gtcgctcgcc gcaagagaaa tcaagccgca ttttcgtgcg gcacaaaatg agaatggaca
     2161 acagaagagg ttttgttgcc tgcatggggc agggaacacg catactttca accggccgtt
     2221 cgcgagagga aaaacccaaa aggcctcccg taactctggc ttcccgcgcg tctttgctgc
     2281 caaagcgcgt caggctcacc cggtacaatc agtgcgttca attccccagg cgaccgcgtt
     2341 gccgcggtca atgcttgaac acatcgtatg ttggacgatt tcaatccgca acatgattga
     2401 aggtcgcgga tatttcgcgg gacgaaaagg actcaggcga tcgtctcgac gtcccatttc
     2461 ggcgcccgct cgggcgggtc ggcgatgcg
//
LOCUS       DASSRL010000460         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1569624, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000460 DASSRL010000000
VERSION     DASSRL010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569624"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..838)
                     /locus_tag="VFL49_09360"
     CDS             complement(23..838)
                     /locus_tag="VFL49_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912229.1"
                     /translation="MLPILRILPVGGVLLAIFILVLALSPPDGSRAPLNTTIAPARGA
                     LVVREHHPETRQFLIMAALKRADELNRLRDLPDTPTKTEPVPTEPVAVEPPATESTVE
                     EPKVAGLPADRTESDPEPEEPTATETPSVSIPVETGEVSAPELPVSPPETNSTSQKPE
                     QVREQIKPQQREIRRRAHRPRRPRATASAPQRPFNLFEVLFGGQQYRPSPQTYASQQS
                     AQRQYQPQQPAYAAQSAQRQYQQPAYNPQPQLPTPPALPAPTEPYPMLRQHAY"
     gene            1019..1984
                     /locus_tag="VFL49_09365"
     CDS             1019..1984
                     /locus_tag="VFL49_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7912230.1"
                     /translation="MMKKPNCPVIAIEEHYWDPELARHFIGPEAGRGNETDQRLFDLG
                     SLRLKEMDEAGIDVQVLSHGAPSTQKLGADIAADLTRQVNDRLAKVCAANPKRFAAFA
                     ALPTVDPKAAADELERTVTRLGFKGAMLHGMSNGEFLDLRKFWPIYERAEKLDVPIYF
                     HPSLPHAKVTEIYYQDYAKDFPLVVRPAWGYTVETATQAIRLVLSGVFDTHPNLKIIL
                     GHFGETLPFLLWRIDSSLKRPGQKAMSFRDIFCKNFYVTTSGFFSDPALICCMLEMGV
                     DHILFAVDWPFVMNPPAVEWMQNVSISDSDKVKILSGNAQRLLKM"
     gene            complement(1985..2389)
                     /locus_tag="VFL49_09370"
     CDS             complement(1985..2389)
                     /locus_tag="VFL49_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7912231.1"
                     /translation="MHVFSLEREQQWDPKHHVEKILGKIADGDVTVACWEPGQISPYH
                     CHPEATEIYFCFTGGGTIRTPQQTVPVTPGAFIVHPPGEVHEYENGPARTLLFRVRYG
                     ADMRAHHVEWRGNGGWKQSAADADYYRRHPLA"
     gene            2495..2571
                     /locus_tag="VFL49_09375"
     tRNA            2495..2571
                     /locus_tag="VFL49_09375"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2529..2531,aa:Ile,seq:cat)
BASE COUNT          465 a          797 c          865 g          529 t
ORIGIN      
        1 ttattactaa gctgccgtcg cgtcagtagg cgtgctggcg caacatcgga tacggctcgg
       61 tcggcgccgg caatgccgga ggcgtcggca gctgcggctg cggattatat gccggttgct
      121 gatactgcct ttgcgctgac tgtgcagcgt aagcgggctg ttgcggctgg tactgccgct
      181 gagcggattg ctggctggca taggtttgcg gcgacggccg atattgctgt ccgccgaaca
      241 gcacctcgaa aagattgaag gggcgttgcg gcgcgctggc tgtcgctctg gggcgacgcg
      301 gccgatgagc gcgtcgccgg atctcgcgct gctgcggctt tatctgctct ctgacctgtt
      361 ccggtttctg acttgttgaa tttgtctccg gcggcgacac cggcaattcg ggtgccgaca
      421 cctcaccggt ctccacggga atgctcacgc ttggcgtctc ggttgcggtc ggctcctcgg
      481 gctccggatc cgactcggtc cggtcggccg gaagtccggc gaccttcggc tcttccacag
      541 tggattcagt tgcggggggc tcaacggcga caggctccgt cgggacgggc tcggtttttg
      601 tcggcgtatc gggcaagtcg cgcagccggt tgagctcatc ggcgcgcttc agtgcggcca
      661 tgatcagaaa ctggcgcgtc tccggatgat gctcgcgcac gacgagcgcg ccgcgcgcgg
      721 gagcaatcgt cgtattcaaa ggcgcgcgcg aaccatccgg cgggctgagc gccaggacca
      781 ggataaaaat ggcgagaagt acgccgccca ctggaagaat acggagaatc ggcagcatcg
      841 tccgtgcata actgagtcgg gaacaatacc atagcccggt tccccggctc gccggcagca
      901 cgccggcgca tgaggcattt ttgccacggc gtcgtcattt gcctggtcgt cgggccgtga
      961 caggggaaat cgccgttgct aggtaacggt tccgaaaaaa gtgccgcggg gaaacaccat
     1021 gatgaaaaag ccgaactgcc cggtcatagc gatcgaagaa cactattggg atccggaact
     1081 tgccaggcat ttcatcggcc cggaagccgg ccgcggcaac gagaccgacc agcgtctttt
     1141 cgacctcggc agcctgcgcc tcaaggaaat ggatgaggcc ggcatcgatg ttcaggtgct
     1201 ttcgcatggg gcgccttcga cgcagaagct gggtgccgat attgcagccg acctgacgcg
     1261 ccaggtgaat gaccgcctcg ccaaggtctg tgccgccaac ccgaagcgct tcgccgcttt
     1321 cgccgcactt ccgaccgtcg accccaaggc cgcggcggac gaactcgagc gcaccgtcac
     1381 caggctcggc ttcaagggtg cgatgctgca cggcatgtcg aacggcgagt ttctcgatct
     1441 caggaagttc tggccgatct atgagcgcgc cgagaagctg gacgtgccca tctattttca
     1501 cccgtcgctg ccgcacgcga aggtgacgga gatctactat caggactacg ccaaggactt
     1561 cccgttggtg gtgcgcccgg cctggggcta cacggtcgag acggcgacgc aggcgatccg
     1621 cctcgtgctc tcgggcgttt tcgatacgca tccgaacctg aagatcatcc ttggacactt
     1681 cggcgagacg ctgccgtttc tgctgtggcg catcgattca tcactcaagc ggcccggcca
     1741 gaaggcgatg agcttccgcg acatcttctg caagaacttc tacgtcacca ccagcggctt
     1801 cttttccgac ccggcgctga tctgctgcat gctggagatg ggagtcgatc acatcctgtt
     1861 cgcggtcgat tggccgttcg tgatgaatcc gccggcagtc gaatggatgc agaacgtgtc
     1921 gatatcggat tccgacaagg tgaagatcct gagcggaaac gcgcagcgcc tgctgaagat
     1981 gtgatcaggc gagcgggtgg cggcggtagt agtccgcatc ggcggctgac tgcttccatc
     2041 cgccgttgcc gcgccattcg acatggtgcg cccgcatgtc ggcgccgtag cgtacccgga
     2101 acagcaaggt ccgcgccgga ccgttctcat attcgtgcac ttcgccgggc ggatgaacga
     2161 tgaaagcgcc cggcgtcacg ggaacggtct gctgcggggt gcgtatcgtg cctccgccgg
     2221 tgaagcagaa gtagatttcg gtggcctcgg gatggcagtg ataggggctg atctggccgg
     2281 gctcccagca ggcgacggtc acgtcaccgt cggcgatctt gccgagaatc ttctcgacgt
     2341 gatgcttggg gtcccactgc tgctcgcgtt ccaggctgaa gacatgcatg gcatcggcgc
     2401 tccggcaaac gatacattga gcctagcgca agccttggac ggacaggagg gcggcgttgc
     2461 gcatcggcca cgcgcgttga tagaaaggcc gcacgggccc gtagctcaac tggttagagc
     2521 cgaccgctca taacggtctg gttggaggtt cgagtcctcc cgggcccacc acgcagtcct
     2581 gcgtgtgcgg gatttattgt tctggataca cactattcgg cctgtttttg tggtccgtcc
     2641 gttcagcctg tctgcc
//
LOCUS       DASSRL010000461         5865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_397170, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000461 DASSRL010000000
VERSION     DASSRL010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397170"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /gene="typA"
                     /locus_tag="VFL49_09380"
     CDS             <1..1648
                     /gene="typA"
                     /locus_tag="VFL49_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7912232.1"
                     /translation="RIGDVKVNLVDTPGHADFGGEVERILNMVDGALVLVDAAEGPLP
                     QTKFVVSKALKMGLKPIVVINKVDRSDARPIEVVNEVFDLFAALDATDEQLDFPILYG
                     SAKQGWMATSLEGSHSDGMKPLFDLILSHVKPPQVEDGPFRMLGTILEANPYLGRIVT
                     GRITSGTARPNQSVKVLDRDGNIVETGRITKLLAFRGLERTGVEEAEAGDIVAVAGLP
                     SATVSNTICAPDVETPLPAQPIDPPTLAMIFRVNDSPLAGTEGDKVTGRMIRDRLLRE
                     AEGNVALRVRESDEKDSMEVAGRGELQLGILIETMRREGFELSVSRPKVLLQEDPATG
                     DLLEPIEEVVIDLDEEHSGIVVQKLAERKADMMEMRPSGGGRLRLVFHAPTRGLIGYQ
                     GELLTDTRGTAIMNRLFHAYALYKGQIQGRRNGVLISNDRGEAVAYALWNLEDRGPMM
                     IEPGWKVYSGMIVGEHTRGNDLIVNVLKGKKLTNIRTTSKDEAVRLMPPIRMTLEKAL
                     AYIEDDELVEVTPKSIRLRKKLLDENDRKKEERKKEAETV"
     gene            1871..2380
                     /locus_tag="VFL49_09385"
     CDS             1871..2380
                     /locus_tag="VFL49_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7912233.1"
                     /translation="MSTTLIEIRRAKSSDAVDVAETHDEAWRTAYQGIIPGNELDKLI
                     NRRGPDWWDSAIRKGSRITILQFGDRVAGYANYGRNRARSLFYDGEIYELYLRPEFQG
                     LGFGRRLFTAARKDLNQSGLKSLVVWALSDNEPAMDFYRALGGRAVARSSERFGAKTL
                     DKVAYAWQA"
     gene            complement(2381..3265)
                     /gene="folD"
                     /locus_tag="VFL49_09390"
     CDS             complement(2381..3265)
                     /gene="folD"
                     /locus_tag="VFL49_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET7912234.1"
                     /translation="MSARIIDGKTIAADLRGRITDAVHRLRRDRGIVPGIAVVLVGDN
                     AASEVYVRNKSKAVAESGMRAFDHKLPASTSQSDLLALVSELNANEEISGILVQLPLP
                     KQIEAQKIIAAIDPAKDVDGFHPLNVGRLASGLPGLVPCTPMGCVLLAKTVHESLAGL
                     AAVVIGRSNIVGKPVAQLLLAENATVTVAHSKTNDLPAVCRGADVLVAAIGRAEMVRG
                     DWIKPGATVIDVGINGVPGEGGKSRIVGDVAYAEAAQVAGAITPVPGGVGPMTIACLL
                     LNTLRAACAQKGLPAPKV"
     gene            complement(3291..3611)
                     /locus_tag="VFL49_09395"
     CDS             complement(3291..3611)
                     /locus_tag="VFL49_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HET7912235.1"
                     /translation="MAVVPWSPAAEGVALAVRLTPKAGRDLIDGIEIVSDGRSVLKIR
                     VRATASEGEANAALIKLIARATGVPPRDVNLAAGATSRIKRLVISGDGPTLIAALEKI
                     IAPR"
     gene            complement(3615..3905)
                     /locus_tag="VFL49_09400"
     CDS             complement(3615..3905)
                     /locus_tag="VFL49_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7912236.1"
                     /translation="MRAFLDIILLVLQIYIWLLIAAAVLSWLIAFNVVNARNQVVATI
                     GEFLYRITEPFLRPIRALLPNLGGIDISPVILILVILFLENVIIRYIYPNVF"
     gene            4085..4160
                     /locus_tag="VFL49_09405"
     tRNA            4085..4160
                     /locus_tag="VFL49_09405"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4118..4120,aa:Ala,seq:cgc)
     gene            complement(4165..5649)
                     /locus_tag="VFL49_09410"
     CDS             complement(4165..5649)
                     /locus_tag="VFL49_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7912237.1"
                     /translation="MVDSEKSAPVTERPVPAALGAMLRASDGILDLLPIATFICDAKG
                     TILQYNRHAAAVWGRAPQSGQTHDEFTQSSRFFELDGTPASRSMVSEVLTTGKPVRDV
                     ERIVERADGASLIVSVSIDPLHDAKGNLVGAVTCFLDVTERKRMDNALERSRLYALEQ
                     DQRFAATYEHAAIGMSEIAPDGRFLRVNEAICAITGFSREHLLANMLFTHTHPDDADP
                     DRQAFRKQVAGELDFYSIEKRFIRRDGRVIWISVRSSPVRDAGGRLLYLVRVVQDITE
                     RKAAEQRQRLLMDELNHRVKNTLATVQSLAAQTARAAVTPAAFRERFEGRLIALSKAH
                     DQLTMHHWESADLRELLSGSLAPYAGATPDRVVLRGEDVVLRPRAVLTLAMAVHELTT
                     NAAKYGALSAPGGRVEVQWRPVLAENGHWYLQIEWIEQGGPKVIEPEQHGFGTKLIEG
                     SIAAELGGEARLVFQSSGLRCEISMPMETATVDFRAKAGEEKPT"
BASE COUNT         1173 a         1834 c         1790 g         1068 t
ORIGIN      
        1 ccgcatcggc gacgtgaagg tgaacctggt cgacacgccg ggccacgccg acttcggcgg
       61 cgaggtcgag cgcatcctca acatggtcga cggcgcgctg gtgctggtcg atgccgccga
      121 ggggccgctg ccgcagacca agttcgtggt ctcgaaagcg ctgaagatgg ggctgaagcc
      181 gatcgtggtg atcaacaaag tcgatcgctc cgatgcccgg ccgatcgagg tggtgaacga
      241 ggtgttcgac ctgttcgccg cgctcgacgc gaccgatgag cagctcgact tcccgatcct
      301 gtacggctcc gccaagcagg gctggatggc gacctcgctc gaaggatcgc actccgacgg
      361 catgaagccg ctgttcgacc tcattctcag ccacgtgaag ccgccgcagg tcgaggacgg
      421 gcctttccgc atgctcggca cgatcctcga ggccaatccc tatctcggcc gcatcgtcac
      481 cggtcgcatt acatcgggca ccgccaggcc gaaccagagc gtcaaggtgc tcgaccgcga
      541 tggcaacatc gtcgaaaccg gtcgcatcac aaagctgctg gcattccgcg gccttgagcg
      601 gaccggcgtc gaggaggccg aggccggcga catcgtcgcc gtcgcgggac tgccgagcgc
      661 aaccgtgtca aacaccatct gcgctcccga cgttgagacg ccgcttcccg cccaaccgat
      721 tgatccgccg acgctggcga tgatctttcg cgtcaacgat tctccgctcg ccggcaccga
      781 aggcgacaag gtgaccggcc gcatgattcg cgatcgactg ctgcgcgaag ctgagggcaa
      841 cgtcgctttg cgcgttcgcg aatccgacga aaaggattcc atggaagtcg ccgggcgcgg
      901 cgaattgcag ctcggcattc tcatcgaaac gatgcggcgc gaaggtttcg agctctcggt
      961 gtcgcggcca aaggtcctgc tgcaggaaga cccggctacc ggcgacttgc tcgagccgat
     1021 cgaggaagtt gtcatcgatc tcgatgagga gcattccggg atcgtcgtgc aaaagttggc
     1081 ggagcgcaaa gctgacatga tggaaatgag gccgtcgggc ggagggcgcc ttcgcctggt
     1141 gtttcatgct ccgacacgcg gccttatcgg ctaccagggc gaattgctca ccgacacgcg
     1201 cggcacggcg atcatgaatc ggctgttcca tgcttacgcg ctgtacaagg gacaaatcca
     1261 gggccgccgc aacggcgttc tcatttctaa cgaccggggc gaggccgttg cctatgcgct
     1321 gtggaatctg gaagaccgcg gaccgatgat gatcgagccc ggctggaaag tttacagcgg
     1381 catgatcgtc ggcgagcaca cccgcggcaa cgatctcatc gtcaatgtgc tcaagggcaa
     1441 gaagctgacc aacatccgca ccacgtccaa ggacgaggct gtgcggttga tgcccccgat
     1501 ccgcatgaca ctcgagaagg cgctcgccta tatcgaggac gacgaactgg tcgaagtgac
     1561 gccaaagtcg atccggttgc gcaagaagct gctggacgag aatgaccgca agaaggaaga
     1621 gcggaagaag gaagccgaga cggtctgacg ctccgcgggg gcgaatcgaa tcagcgggca
     1681 ttcctggttg gcaaaacggc gcggcggata gcggaaccgg atgattcggc ctagccggcc
     1741 tgatgtctca agcatccaat ttggtcccgc ctgtccggca ttttggcatc tgtgaccagt
     1801 aatccacacc gttaacccat cattaacgat agggcttgaa ggccggaaat ttcgttgctt
     1861 ctatcgcggc atgagcacga ccctcataga gattcgccgc gcgaagtctt cggacgcggt
     1921 cgacgtcgcc gagacgcacg acgaggcctg gcggactgcc tatcagggaa tcatcccggg
     1981 caacgagctc gacaagctga tcaaccggcg cggacccgac tggtgggact cggcgatccg
     2041 caaaggcagc cgtatcacca tcctgcagtt cggcgatcgc gtcgcaggct atgccaacta
     2101 cggccgcaac cgcgcacgca gcctgttcta cgacggtgaa atctacgagc tctatctgcg
     2161 accggagttc cagggtctcg gtttcgggcg ccgcctgttc actgcggcgc gcaaggacct
     2221 caatcagagc gggctgaaga gcctggtcgt gtgggcgctg tccgacaacg agccggcgat
     2281 ggatttctat cgcgccctcg gcggccgcgc cgtcgcgcga tcttcggagc gcttcggcgc
     2341 aaagacgctc gataaggtcg cctacgcctg gcaggcgtaa tcacaccttc ggcgccggca
     2401 ggcccttctg cgcgcatgcg gcgcgcaagg tattcaacag cagacacgcg atcgtcatcg
     2461 gcccaacgcc gcccggcacc ggggtgatcg cgccggcgac ttgcgccgcc tccgcatagg
     2521 cgacatcacc cacgatccgc gatttgcctc cctcgcccgg cacgccattg atgccgacgt
     2581 cgatgaccgt tgcgcccggc ttgatccagt cgccacggac catctcggcg cggccgatcg
     2641 cagcgacgag cacatcggcg ccccggcaca cagcgggcaa atcattcgtt ttcgaatgcg
     2701 cgaccgttac cgtggcgttt tccgcaagca gcaattgcgc gaccggcttg ccgacgatgt
     2761 tggaccgtcc gatcaccacg gcggcgaggc cggcgagcga ttcatgcaca gttttcgcca
     2821 gcagcacgca gcccatcggc gtgcacggaa caagacccgg caggccgctg gccaatcggc
     2881 cgacattgag cggatggaag ccgtcgacgt ctttcgccgg atcgatggcc gcaatgattt
     2941 tttgcgcctc gatctgcttc ggcaacggca gctgcacgag gatgccgctt atctcttcgt
     3001 ttgcattgag ttctgacaca agggcgagca ggtcggattg gctcgtactc gccggtaatt
     3061 tatggtcgaa ggcacgcatg cctgactcgg ccactgcctt ggacttgttg cggacataaa
     3121 cttcgctcgc tgcattgtcg ccgacgagca cgacggcaat gcccggcacg atgccgcggt
     3181 cgcggcgcag gcgatgcacg gcatcggtaa ttctgccacg caagtccgca gcgatcgttt
     3241 ttccgtcgat aatacgagcg ctcatggtcc gttgccccga cgcacaagcc ttagcgcggc
     3301 gcgataattt tctccagcgc cgcaatgagc gttgggccat cgcccgaaat gaccagccgc
     3361 ttgatgcggg aagtggcgcc tgccgccagg ttcacgtcgc gcggcggaac gccggtagcc
     3421 ctggcgatca gcttgatcag ggccgcgttg gcctcgcctt cgctcgctgt tgcccgcacc
     3481 ctgattttaa ggaccgagcg tccatccgac acgatctcga tgccgtcgat gagatcacgg
     3541 cctgccttcg gcgtcaggcg gacggcaagc gcaacgcctt cggcagccgg cgaccaaggc
     3601 acgaccgcca ttggctagaa cacgttcgga tagatgtacc ggatgatgac gttctcgagg
     3661 aacagaatga ccagaatcag aatcaccggt gaaatatcga tgccgcccag attgggcagc
     3721 agcgcgcgga tcggccgcaa gaacggctcg gtaatccggt aaaggaattc accgatcgtg
     3781 gccacaacct ggttgcgggc gttcaccacg ttgaaagcga tcagccagga taacactgcg
     3841 gccgcgatca ggagccagat gtaaatctgc aaaaccagaa ggataatatc gagaaacgcg
     3901 cgcatgaccg tccgcctgtt cgaaatcagg gcgcaaagta gcgggtggat tgatgccgaa
     3961 caagccaaag ccaaacacga ctacgcgcga tcgcacatcc ttgacaggcc atacccctgg
     4021 ccctaaatgg cgcccactcg ccgcagtatt ccacgcaacg ttgtcggtgt tgctgttgtt
     4081 tggcggggcc gtagctcagt tgggagagcg cctcgttcgc aatgaggagg tcaggggttc
     4141 aaatcccctc ggctccacca gccctcaggt cggcttctcc tcgcccgctt ttgcgcgaaa
     4201 gtcgaccgtt gcggtttcca tcggcatcga gatttcgcag cgtaatccgc tcgattggaa
     4261 gaccaggcgc gcctcgccac caagctcggc agcgatgctt ccttcgataa gcttggttcc
     4321 aaaaccgtgc tgctcgggtt caatgacttt cgggccacct tgctcgatcc attcaatctg
     4381 caagtaccaa tggccgtttt ctgcaagaac cggacgccat tgaacttcga cccgcccacc
     4441 tggcgccgaa agcgcaccat atttggcggc attcgtcgtc agctcatgca cggccatcgc
     4501 aagcgtcagg acggcacgcg gccgcaatac gacgtcctcg ccgcgaagga cgactcggtc
     4561 gggtgtagcc ccggcataag gtgcaagact gccggacaac agctcacgca aatccgcact
     4621 ttcccagtgg tgcatagtga gctgatcgtg agccttcgac aatgcgatca gcctgccctc
     4681 gaaacgctcg cggaaggcgg ccggcgtaac agcggcgcgc gctgtctggg cggccagcga
     4741 ttgcaccgtt gcgagcgtgt tcttcacgcg gtggttaagt tcgtccatca gcaggcgttg
     4801 ccgttgctct gccgccttgc gttcagtgat atcctgaacg acgcggacaa gataaagcag
     4861 gcggccgccc gcatcacgga ccggcgaaga gcgcactgaa atccagatca cccggccatc
     4921 acgccggatg aatcgttttt caatcgaata gaagtcgagc tcgcccgcaa cctgcttgcg
     4981 gaacgcctgt cggtccggat cggcgtcgtc gggatgcgtg tgggtgaaaa gcatgttcgc
     5041 gagcagatgc tcgcggctaa accccgttat tgcacagatc gcctcgttga cgcggagaaa
     5101 gcggccatcg ggagcgattt ccgacattcc gatggcggca tgctcatagg ttgcggcaaa
     5161 gcgctggtcc tgctcgagcg catatagtcg tgaacgttcc agcgcgttat ccattcgctt
     5221 gcgctctgtg acgtcgagaa aacaggtcac cgcaccaacc aggttgcctt tcgcgtcgtg
     5281 caacgggtcg atgctcaccg acacgatgag gcttgcaccg tcggcgcgct caacgattcg
     5341 ctccacgtcg cggactggct tgccggttgt cagcacctcg gaaaccatgg agcgcgacgc
     5401 cggcgtcccg tcgagttcga aaaagcgtga gctctgcgta aactcgtcgt gcgtttgccc
     5461 ggactgtggt gcgcggcccc agaccgccgc ggcatgacgg ttgtattgaa gaatggtgcc
     5521 tttggcgtcg cagataaacg tcgcaatcgg cagaaggtcg agaataccgt ctgacgcacg
     5581 cagcatagcg cccagtgcgg ccggcactgg ccgctcggtg acaggggcgg acttctccga
     5641 atccaccatc ctcacgcctc cgcgtggaca ttccacccct aaccccgccg caacgatcag
     5701 tgcgaatggc gatttgtcaa tggcgtgatg aggtgcgcaa atttaccggt caggacgccg
     5761 tcttcttttc ggcctgactg gccggcacaa ctctcatcca gtgatcgata aaggcagcga
     5821 gccagttgcg agcagcatga tcgtgaaccg cgcggtcagc gatat
//
LOCUS       DASSRL010000462         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1289570, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000462 DASSRL010000000
VERSION     DASSRL010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289570"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFL49_09415"
     CDS             <1..870
                     /locus_tag="VFL49_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7912238.1"
                     /translation="TTPSSDQPNVDVAYGAFQRGFYLTAFSEATKRAQQNDPASMTLL
                     GELYAQGLGIGRDDSKAAQWYKLAAAKGDRDALFALAMFSFQGRAGPRNLEEAARLLD
                     AAAKLGHPGAAYDLGLLYLQGQQFPQDFKRAAELFATASEAGNAEAQYALATMYKEGR
                     GVAKDLGKATRLMQQASIGGNLDAMVEFAIAQFNGEGTAKDEAAAARLFLKAAHLGSP
                     IAQNRLARILMAGRGMPADPTEAVKWHLVAKAGGNSDPDLDVFASKQTQAVRDAADKA
                     AKKWLSTAVARRP"
     gene            905..1702
                     /locus_tag="VFL49_09420"
     CDS             905..1702
                     /locus_tag="VFL49_09420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7912239.1"
                     /translation="MQHSALLNVMIAAARKAARAIKRDFGELEKLQVSLKSPANFVTA
                     ADRRAEETIYTELIKARPGYGFLGEESGRREGSDKSHTWIVDPLDGTTNFLHGIPHFA
                     TSIALERDGGVVAGLIYNPATDEMFIAEKGKGAFLNDRRIRVAARTRLAESIVACGIA
                     HHGHGDVDIWRKEIGAVQEHVAGVRRFGAAALDLAWVASGRLDGYWERTLKPWDMAAG
                     LVLVREAGGFVSDCDGREEIFGKGHVVAGNDPIHKSLLSLLKTADKR"
     gene            1833..2810
                     /locus_tag="VFL49_09425"
     CDS             1833..2810
                     /locus_tag="VFL49_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HET7912240.1"
                     /translation="MAKELDPLKLSSPRIFLFRMLVFVILVGLIAFVLHKQIEVAFMS
                     NPGLNALIIGVEAVGIILSFRQVLRLYPEIAWVNNFRLVDPGLAVDRPPVLLAPMAAI
                     LGSRVGRMAMSAQLMRGILDSIATRLDEARDISRYMTGLLVFLGLLGTFWGLIETVSS
                     VGNVIQGLKASGDTGSMFDSLREGLAAPLSGMGISFSSSLFGLAGSLVLGFLDLQSSQ
                     AQNRFYTELEDWLSTTVYDQAAEPAIAAGMGGEVRSAIERLREAVDQTGANKSASAAM
                     ANLAEAIQGLVHHMRTEQQMIRDWVDSQADQQREIKKLLEVMVREHVNR"
BASE COUNT          532 a          870 c          882 g          527 t
ORIGIN      
        1 acaacgccca gctccgatca gccgaatgtc gacgtcgctt atggcgcctt tcagcgggga
       61 ttctacctta ctgctttcag tgaggcgacg aaacgtgcgc agcaaaatga tcctgcatcg
      121 atgacgctgc tcggggagct ctacgcgcag ggccttggta tcggacgcga cgattcaaaa
      181 gcggcgcaat ggtacaagct tgccgcagcc aaaggcgacc gcgatgccct ctttgcgctt
      241 gcaatgttca gtttccaggg ccgcgccggc ccgcgcaatc tcgaagaggc cgcgcgcctg
      301 ctggatgccg ccgccaagct tggacacccc ggcgccgcct atgatctcgg gctgctttat
      361 cttcagggcc agcaatttcc gcaggacttt aaacgcgccg ccgagctgtt cgcgacagca
      421 tccgaggcgg gcaatgccga agcgcagtat gcactcgcca cgatgtataa agagggccgc
      481 ggcgtggcga aggatctcgg caaggcgacg cgcctcatgc agcaggcgtc catcggcggc
      541 aacctcgatg cgatggtcga attcgcaatc gctcagttca acggagaagg cacggccaaa
      601 gacgaagccg ccgcggcacg tctctttctg aaggcagcgc atcttggcag tccgatcgcg
      661 caaaaccggt tggcgcggat actcatggcc ggccgcggca tgcctgccga tccgaccgaa
      721 gccgtcaaat ggcatctcgt cgccaaggcc ggtggcaaca gtgatcccga tctcgacgtc
      781 ttcgcttcaa aacagaccca ggctgtgcgc gacgcagccg acaaggccgc gaagaaatgg
      841 ctgtcgacgg cagtcgcgcg gcggccttga cgacgggcgc cttcacgggc agaccgcagg
      901 cgccatgcag cattctgctc tgctcaacgt gatgatcgcc gcagcgcgca aggccgcgcg
      961 cgcgatcaag cgcgacttcg gcgagctgga aaaattgcag gtctcgctca aaagcccggc
     1021 caatttcgtg acagcggcgg accgccgcgc cgaggaaacg atctacactg aactcatcaa
     1081 ggcgcggcca ggttatggtt tccttggaga ggaaagcggc cgtcgcgagg gcagcgacaa
     1141 gagtcatacc tggatcgtcg acccgctcga tggcacgacc aatttcctgc atggcatacc
     1201 tcacttcgca acgtcgattg cgctggagcg cgacggcggc gtcgtggccg gcctgattta
     1261 caacccggcg accgacgaga tgttcatcgc cgagaaaggc aaaggcgcgt ttctcaacga
     1321 tcgtcgcatc cgtgtcgcag cacggacacg gctcgcggag tcgatcgtgg cttgcggaat
     1381 tgcccaccac ggccatggcg acgtcgatat ctggcggaag gaaatcggcg cggtgcagga
     1441 acatgtggcc ggcgtgcgcc gctttggcgc cgcggcgctt gatctcgcct gggtggcctc
     1501 gggccggctc gacggctact gggagcggac cttgaagcct tgggacatgg cggcgggact
     1561 ggtgcttgtg cgcgaggccg gcggtttcgt ctccgattgc gacggccggg aagagatttt
     1621 cggtaaaggc cacgtcgtcg ccggcaatga cccgatccac aaaagcttgt tgtcgttgct
     1681 gaagacggcc gacaaacgct aggcaccgca atttcaactc aatcggccgc tttaccgagg
     1741 ttcgccgggg ttccggcaca actgcttttt acacgtcgct tgtctgtgtc atagtgcgtc
     1801 caggggggcg ttcaggcgca acgacgaaac tcatggccaa agaactggat cctctcaagc
     1861 tgtcgtctcc gcggatcttc ctgttcagga tgctggtatt cgtcatcctg gtgggactga
     1921 tcgcgttcgt actgcacaag cagatcgaag tcgctttcat gtccaacccg gggctgaatg
     1981 ccctgatcat aggtgtcgaa gcggtcggca ttatcctgtc gttccggcag gtgttgcgcc
     2041 tttatccgga aatcgcctgg gtcaacaatt tccgcctggt cgatccgggc cttgccgtcg
     2101 accggccgcc cgtactgctt gcgccgatgg cagccatcct cggcagccgc gtcggccgca
     2161 tggcaatgtc cgcgcaattg atgcgcggca tcctcgattc gatcgccacc cgactcgacg
     2221 aggcgcgcga catttcccgc tatatgaccg gtctgctggt ctttctcggc ctgctcggta
     2281 ccttctgggg gctgatcgaa accgtcagct cggtcggaaa cgtcattcaa ggcctcaagg
     2341 cgagcggcga caccggcagc atgttcgact cgctgcgcga agggctcgcc gcaccgcttt
     2401 ccggcatggg catttcgttt tcgtcgtccc tcttcgggct tgcaggctcg ctcgttctcg
     2461 gcttcctcga tctgcagtcg agccaggcgc agaaccgttt ctacaccgag ctggaagact
     2521 ggctatccac caccgtttac gaccaggctg cggaacccgc tatcgccgcg ggcatgggcg
     2581 gcgaggttcg ttccgctatc gagcggctgc gcgaggcggt tgaccagacg ggcgcaaaca
     2641 agtccgcctc cgcggccatg gccaatttgg cggaggcgat acaggggctg gtgcaccaca
     2701 tgcgcaccga gcagcaaatg ataagggact gggtcgactc gcaggccgat cagcagcgcg
     2761 agatcaagaa gctgctggaa gtgatggtac gcgagcacgt gaaccgttga g
//
LOCUS       DASSRL010000463         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_939663, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000463 DASSRL010000000
VERSION     DASSRL010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939663"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFL49_09430"
     CDS             complement(<1..223)
                     /locus_tag="VFL49_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET7912241.1"
                     /translation="MARTGTSPALHKGPASSPKRSLDETRAIWLEAFRRVRAETERRA
                     SHLSAEDQIVQSMADASPAKWHRAHVTWFF"
     gene            complement(488..564)
                     /locus_tag="VFL49_09435"
     tRNA            complement(488..564)
                     /locus_tag="VFL49_09435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(528..530),aa:Met,seq:cat)
     gene            complement(651..1016)
                     /locus_tag="VFL49_09440"
     CDS             complement(651..1016)
                     /locus_tag="VFL49_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912242.1"
                     /translation="MACETRAVGLLVAALLLPTVAAAQAPPPTKTPVAPKVDQLDPNA
                     CAKSDTQETVGKGAEAGSHRQDGKDDLSEKLARSGGVICPPEHVDPEIKQPTPPTGGS
                     MPVIPPPGSPGGDQSVRPK"
     gene            complement(1086..1748)
                     /locus_tag="VFL49_09445"
     CDS             complement(1086..1748)
                     /locus_tag="VFL49_09445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7912243.1"
                     /translation="MGADIDKTSVERAYARWAPVYDLVFGAVFDTGRRAAIAAAEQAC
                     GDKCGRILEVGVGTGISLPDYSKKNRLLGIDLSEPMLRKAHERVTEHNLTNVETLAVM
                     DAERMALPDRSFDVVVAQFVITAVPNPEATLNEFARVIRPGGEIVLVNHIGAEAGPRR
                     AFEVCFAPIARRLGWRPEFRFGRLADWAERHGGVRVVEHRPMPPLGHFSLIRFERLAN
                     GN"
     gene            complement(1757..2950)
                     /gene="mnmA"
                     /locus_tag="VFL49_09450"
     CDS             complement(1757..2950)
                     /gene="mnmA"
                     /locus_tag="VFL49_09450"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7912244.1"
                     /translation="MANSLELDCSPAATRVVVAMSGGVDSSVTAALLKAEGYDVVGVT
                     LQLYDHGATAHRKGACCAGQDIYDARRVAERIGIPHYVLDYETRFKEAVIDRFAESYI
                     QGETPVPCVDCNMSIKFRDLLITARELGADVLATGHYVASRSLPGGGRALHRARDQER
                     DQSYFLFGTTREQLDVLRFPLGEQSKTETRELARRFGLAIADKHDSQDICFVPTGHYA
                     DIIERLKPGAAEGGDIVDLEGKVLGQHSGIIHFTVGQRRGLGVATGTPLYVVRLDAEQ
                     RRVIVGPREALRMSRIVLRDVNWIGEGSLEEALADDRREVFVKVRSTRPPQAAWLRHG
                     VNGCEVELVDCEHGVSPGQACVFYDAAEGQARVLGGGFIKSAVAAGDVRSAGDARAAV
                     PAMRS"
     gene            3161..>3724
                     /locus_tag="VFL49_09455"
     CDS             3161..>3724
                     /locus_tag="VFL49_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912245.1"
                     /translation="MPQVASDPKVHAASFHTPRVARPAPPDRAPSPFESLIEDTSPPA
                     EPAPPPESKVTRSDDKPAPAKSTDCKPAEPNDDPKPAETDNVAGEPAVDDSTAEAVGK
                     KTAKPRTAAGVGDSIQAGAEDEPATEQKTSDAGTTTTDGAVTVTSSEAISIVPAPAQV
                     PNTENQQTEQPLQQLALVADTKPKMMQL"
BASE COUNT          748 a         1202 c         1130 g          644 t
ORIGIN      
        1 cgaagaacca ggtgacgtgc gcgcggtgcc acttggcggg gcttgcatcc gccatcgact
       61 gaacgatctg gtcctcggcc gacagatgcg atgctcgcct ttccgtttcc gcccgcacac
      121 gacgaaaagc ctcaagccag attgcacgag tttcatccag cgaacgtttg ggactgctcg
      181 ccggcccttt gtggagagcc ggcgacgttc cggttcgcgc catggaaatc ctccgtgact
      241 atacaaaggg cttcggcagt cgatgtacgt acagagacga attatggacg caattgttcc
      301 atcgcaagga cgtcgtagca accttgaatt gtctttcatc acttgcctat gggtttgtga
      361 tgttcgatat cgcggactcg aaatgcggca atcctttgat cttcgtgcaa gaaggcggac
      421 cgaatcgcgt cggactccgc gtttacttga aaagattgtc ttttgttatc cgaccggaag
      481 tcggatttgg tagcggggga gggactcgaa cccccgacct ccggattatg attccgctgc
      541 tctaaccagc tgagctaccc cgccattccg aaacaaagcg ccggaatggc cgcgatatag
      601 gaagccgaag caaacactgt caagcaatcg cgcttcagtc agcacagcga ctatttcggc
      661 cgaaccgact ggtcccctcc cgggctgccg ggtggcggga tcaccggcat cgacccgcct
      721 gtcggcgggg tcggttgctt gatctcggga tcgacatgtt ccggcgggca aatgacacca
      781 cccgaacgcg caagcttttc gctcagatcg tcctttccgt cctggcgatg gctcccggct
      841 tcagcgccct tcccgaccgt ttcttgcgtg tccgatttcg cgcaggcgtt cggatcaagc
      901 tggtcgacct tgggcgcgac cggcgttttt gtcggtggcg gcgcctgcgc ggcggccacc
      961 gtcggtagca gcagcgccgc taccagcaga ccgacagcgc gagtttcaca ggccatggca
     1021 gccacctcct ccacatgcgg acatgcaggg cacagataac ggtttgacag gagtgagagt
     1081 tccgctcagt tgccgttggc gagacgctcg aagcggatca gcgagaaatg gccgagcggc
     1141 ggcatcggcc ggtgctccac gacacgcacg ccgccgtggc gttccgccca atcggccaga
     1201 cggccgaagc ggaattccgg ccgccagccg agcctgcgcg cgatcggcgc aaagcagacc
     1261 tcgaaggcac ggcgcggacc ggcttccgcg ccgatgtgat tgaccaggac gatctcgccg
     1321 cccggccgga tcacgcgggc gaactcgtta agcgtcgctt cgggatttgg caccgccgtg
     1381 atgacaaact gtgccaccac gacatcgaac gaccgatcag gcaatgccat gcgctcggcg
     1441 tccatgaccg ccagtgtctc gacattggtg aggttatgct ccgtgacacg ctcgtgcgcc
     1501 tttcgcagca tcggctcgga aaggtcgatg ccgagcaggc gatttttttt cgaataatcg
     1561 ggaagcgaaa tcccggtgcc gactccgacc tcgagaattc gtccgcattt atcgccgcaa
     1621 gcctgctcgg cggcggcgat cgcggcgcgc cggccggtat cgaacaccgc gccgaacacc
     1681 aggtcataga ccggcgccca tcgcgcatag gcgcgctcaa cgcttgtttt gtcgatgtcc
     1741 gcccccacag cgagaactag ctgcgcatcg cgggaaccgc cgcgcgcgca tcgccggcgg
     1801 aacgaacatc gccggccgcg accgcgctct tgatgaaacc gccgcccagg acgcgcgcct
     1861 ggccctcagc cgcgtcataa aagacgcagg cctggccagg cgacaccccg tgctcgcaat
     1921 cgaccaattc cacttcgcag ccattgacac cgtggcgcag ccaggcggcc tgcggcggac
     1981 gggtcgagcg gaccttcacg aagacttcgc ggcgatcgtc ggccagagcc tcctcgagcg
     2041 aaccttcgcc gatccagttg acgtcgcgca gcacgatgcg gctcatgcgc agcgcctcac
     2101 gcgggccgac gatgacacgc cgttgctcgg catcgagccg cacgacatag aggggcgtgc
     2161 cggtggcaac gccgagaccg cgccgctgac ccactgtgaa atgaatgatg ccggagtgct
     2221 ggcccagtac ttttccctca aggtcgacga tatcgccgcc ctccgccgcg cccggtttca
     2281 acctttcgat gatgtcggcg taatggccgg tcggaacgaa gcagatgtcc tgactgtcat
     2341 gcttatcggc aattgccagc ccgaagcgcc gcgcaagctc gcgggtttcc gtcttgcttt
     2401 gctctccaag cgggaagcgc aaaacatcaa gttgctcgcg ggtcgtgccg aaaagaaaat
     2461 agctctggtc gcgctcttgg tcgcgcgcgc gatggagcgc cctgcccccg ccgggcagcg
     2521 aacgtgacgc gacatagtga cctgtcgcca gcacgtcggc gccgagttcg cgcgcggtga
     2581 tcagcagatc gcgaaacttg atcgacatat tgcagtcgac gcagggaacc ggtgtctcgc
     2641 cctgaatgta gctttcggcg aagcggtcga tgacggcttc cttgaagcgc gtctcgtaat
     2701 cgagcacgta atgcggaatg ccgatgcgct ccgcaacgcg gcgggcgtca tagatgtcct
     2761 ggccagcgca gcaggcaccc ttgcgatgcg ccgtcgcgcc atgatcgtaa agctgcagcg
     2821 tcacgccaac cacgtcgtag ccctctgcct tgagcagggc ggccgtcacc gacgagtcga
     2881 caccgccgga catggcgaca acgacgcgcg tagctgcggg actgcaatcg agttcgagac
     2941 tattggccat gactgttctt actacaggtg gcgaccgggg acgcgcaatg cgagcggtaa
     3001 ccggcaaatt ttgccgtgct gttatcacga atgccgggcg acgttcactt ttctcacgta
     3061 tccgcgctcc tcaagcattt gaataaacac acgaattcgg caattccggg gctggcctga
     3121 cgattgcaca ttaatcggcg gaaatccgga ggtccgccca gtgccacagg ttgcgtcaga
     3181 tcccaaagtt catgcggcca gttttcacac gccgcgcgtt gcgcgtccgg cgccgccgga
     3241 ccgcgcgccg tcgccgttcg agagcttgat cgaggatacc tcaccgcctg ccgagcctgc
     3301 gccaccgccg gaaagcaaag ttaccaggag cgatgataag ccggcgcccg ccaaaagcac
     3361 ggactgcaag ccggccgagc cgaacgacga cccgaagccg gcagaaaccg acaatgtggc
     3421 tggcgagcca gcggttgacg acagcacggc tgaggctgtc ggcaagaaga ccgccaaacc
     3481 caggactgcc gccggtgtcg gggacagcat ccaggcagga gccgaagatg agccggctac
     3541 agagcaaaag accagcgatg cggggacgac gacgaccgac ggcgccgtga ccgtcacgag
     3601 ctccgaagcg atctcaatcg taccagcacc ggcacaagtg cccaacacag agaaccagca
     3661 aaccgagcag ccgctgcaac agctggcgct cgttgccgat accaaaccta aaatgatgca
     3721 gctc
//
LOCUS       DASSRL010000464         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_484833, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000464 DASSRL010000000
VERSION     DASSRL010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484833"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2256
                     /gene="rpoB"
                     /locus_tag="VFL49_09460"
     CDS             <1..>2256
                     /gene="rpoB"
                     /locus_tag="VFL49_09460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7912246.1"
                     /translation="LLVQPPEGGRPDEGLQAVFKSVFPISDFSNSSMLEFVRYEFEPP
                     KYDVDECRQRGMTYAAPLKVTLRLIVFDIDEETGARSVKDIKEQDVYMGDIPLMTNNG
                     TFIVNGTERVIVSQMHRSPGVFFDHDKGKTHSSGKLLFAARIIPYRGSWLDIEFDAKD
                     IVYARIDRRRKIPVTSLLYALGLDGEQILATFYKHVMYKRQKDGWRVPFDANRLKGYK
                     AVNNLVDADTGRTVVEAGHKLTVRQARQLADKGLKALRMTDDELIGHYVAEDLANGRT
                     GEIYMEAGEEITEKNLKILIDAGYKELPLLDIDHVNVGAYIRNTLSVDKNMTREDALF
                     DIYRVMRPGEPPTVETAEAMFRSLFFDSERYDLSAVGRVKMNMRLDLDAPDTMRVLRR
                     DDIIAVIRTLVDLRDGKGEIDDIDHLGNRRVRSVGELMENQYRIGLLRMERAIKERMS
                     SVDIDTVMPQDLINAKPAAAAVREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGL
                     TRERAGFEVRDVHPTHYGRICPIETPEGPNIGLINSLATYARVNKYGFVETPYRKVKD
                     GRVTDEVVYLSAMEEARHAVAQANAAIDGRGRFTEDLIVCRQAGDVHLVPRDKVDFMD
                     VSPKQLVSVAAALIPFLENDDANRALMGSNMQRQAVPLVRAEAPFVGTGMEGVVARDS
                     GASIAARRSGVVDQVDATRIVVRATGETDPTKPGVDIYRLMKYQRSNQNTCINQRPLV
                     KVGDNVDKGDII"
BASE COUNT          450 a          699 c          697 g          410 t
ORIGIN      
        1 ctgttggtgc agccgccgga gggcggacgt cccgacgaag gcttgcaggc ggtcttcaaa
       61 tcggtatttc cgatttccga tttttcgaac tcctcgatgc tcgaattcgt tcgctacgaa
      121 ttcgagccgc cgaaatacga cgtcgacgaa tgccgtcagc gcggcatgac atacgccgcg
      181 ccgctcaagg tgacgctgcg tctcatcgtt ttcgatatcg acgaggagac cggtgcgcgt
      241 tcggtcaagg acatcaagga gcaggatgtc tacatgggcg acattccgct catgacgaat
      301 aacggcacat tcatcgtcaa cggcaccgag cgggtgatcg tttcgcagat gcatcgctcg
      361 ccgggcgtgt tcttcgatca cgacaagggc aagacccatt cctcggggaa gctcctgttc
      421 gccgcgcgca ttattcccta tcgcggctcg tggctcgaca tcgagttcga tgcgaaagac
      481 atcgtctatg cgcgtatcga tcggcgccgc aagattcctg tgacgtcgct gctctatgca
      541 ctcggcctcg acggcgagca gattcttgcg acgttctaca agcacgtcat gtacaagcgc
      601 cagaaagacg gctggcgcgt gcctttcgat gccaaccggc tgaaaggcta caaggcggtc
      661 aacaacctgg tcgacgccga caccggccgc accgtcgtcg aagcgggtca caagctcacc
      721 gtgcgccagg cgcgccaact tgccgataaa gggctcaagg cgctgcgcat gaccgatgac
      781 gaactcatcg gccattacgt cgccgaagat ctggccaacg gccgcaccgg cgagatctac
      841 atggaagccg gtgaggagat taccgagaag aacctcaaga tcctgatcga tgccggctac
      901 aaggagttgc cgctgctcga catcgatcac gtcaacgtcg gggcctacat ccgcaacacg
      961 ctgtcggttg acaagaacat gacgcgcgag gacgcgctgt tcgacattta ccgcgtcatg
     1021 cgccccggcg agccaccgac tgtcgaaacg gcggaagcga tgttccgctc attgttcttc
     1081 gacagcgagc gctacgatct ctcggccgtc ggccgcgtga agatgaacat gcgtctcgac
     1141 ctcgacgcgc ctgacacgat gcgcgtgctg cgccgcgacg acatcatcgc ggtgattcgc
     1201 acgctggttg acctgcgcga cggcaagggc gagatcgacg acatcgacca tctcggcaat
     1261 cgccgcgttc gctcggtcgg cgagctgatg gagaaccagt accgcatcgg cctcctgcgc
     1321 atggagcgcg cgatcaagga gcgcatgtcg tcggtcgata tcgataccgt catgccgcag
     1381 gacttgatca atgcgaagcc ggccgccgcc gcggtgcgcg aattcttcgg ctcctcgcag
     1441 ctttcgcagt tcatggacca gaccaatccg ctgtccgaga tcacccataa gcggcgcttg
     1501 tcggcgctcg gcccgggcgg cctgacgcgc gagcgtgcgg gctttgaggt gcgcgacgtg
     1561 cacccgaccc attacggccg catttgcccg atcgaaacgc cggaagggcc gaatatcggt
     1621 ctgatcaatt cgctggcgac ttacgcgaga gtcaacaaat acggcttcgt cgaaacgccg
     1681 tatcgcaagg taaaggacgg ccgcgtcacc gacgaggtgg tttatctgtc ggcgatggag
     1741 gaggcgcgtc acgcggtggc gcaagccaac gcggcaatcg acggccgcgg ccggttcacc
     1801 gaagatctga tcgtctgccg ccaggccggc gacgtgcatc tggtgccgcg cgacaaggtc
     1861 gacttcatgg acgtgtcgcc caagcagctc gtatcggttg ccgccgcgct cattccattc
     1921 ctggagaacg acgacgccaa ccgcgcgctg atgggttcga acatgcagcg ccaggcggtg
     1981 ccgcttgtgc gtgccgaggc gcctttcgtc gggaccggca tggaaggcgt ggtggcgcgc
     2041 gattcaggcg cctcgatcgc cgcacggcgg agtggggtcg tcgatcaggt cgacgccacc
     2101 cgtatcgttg tccgcgcgac aggcgagacc gacccgacca agccgggcgt cgatatttat
     2161 cggctgatga aatatcagcg ctcgaaccag aacacctgca tcaaccagcg cccgctggtg
     2221 aaggtgggtg acaatgtcga caagggcgac attatt
//
LOCUS       DASSRL010000465         6058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_29550, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000465 DASSRL010000000
VERSION     DASSRL010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29550"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="sufD"
                     /locus_tag="VFL49_09465"
     CDS             <1..1074
                     /gene="sufD"
                     /locus_tag="VFL49_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7912247.1"
                     /translation="AQGKVPALPGLSAGRVTIVNGRYEPTWSDFKTAAGVELGDLFAF
                     AAEQKDHRLGDVPGQDDIAMSLNTAFMTGGVALRVAKGADVEKPVEIAHFFSDNSAAA
                     MYPRSVVLVEPGARLTLIESFSGPAGVDYQVNSALELVIGEGAHVDHVTIGHDGDAAL
                     RISTLGLKVGANVIYNGFTYTAGGAVVRNQIFAHFNGKETQASIGGATLLKGRQHVDN
                     TLVINHAAGGCTSRERFRTVLDGESHGIFQGKIIVEPHAQKTDAKMASHALLLSDSAE
                     ADNKPELEIFADDVQCGHGATAGALDRTLKFYLMSRGIPENEAEALLIQAFVGEAIEE
                     IAHEGIREALLNAAAKWLDAREQ"
     gene            1077..2318
                     /locus_tag="VFL49_09470"
     CDS             1077..2318
                     /locus_tag="VFL49_09470"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7912248.1"
                     /translation="MHPAVSNGSYDVAKIRADFPALAMKVYGKPLVYLDNAASAQKPK
                     AVLDRLAKAYTEQYANVHRGLHFLANEATEAYEGARETVRAFLNAERKEEIIFTRNAT
                     EAVNLVAYTFGRERIKAGDEIVLSIMEHHSNIVPWHFLRERQGAVIKWAPIDEDGNFL
                     LDEFEKLLTPRTRMVAVTHMSNALGTAVPVKEVVRIAHARGIPVLVDGAQAAVHLDVD
                     VRDIDCDFYAITGHKLYGPTGIGALYGKYEHLAAMPPFNGGGEMIHEVFEDRITYGEP
                     PHKFEAGTPAIVQAIGFGAAIDYVNSVGKARIRAHEDALVKYAHEQLGKINSLRIIGR
                     APDKGPIVSFEMKGAHPHDVATIIDRAGVAVRAGTHCVMPLLARYGLTATCRASLAMY
                     NTRDEVDALVASLVKAREFFT"
     gene            2315..2713
                     /locus_tag="VFL49_09475"
     CDS             2315..2713
                     /locus_tag="VFL49_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HET7912249.1"
                     /translation="MTDDTTQNAPVAEKPAENAAVTGSSALPQDELNKRTDDIVAALK
                     TVYDPEIPADIYELGLIYRIDIGDDRGVKIDMSLTSPNCPAAQDLPIMVENAVSSVPG
                     VKETKVEVVWDPPWDPSRMSDEARLVLNMW"
     gene            2757..3146
                     /gene="sufA"
                     /locus_tag="VFL49_09480"
     CDS             2757..3146
                     /gene="sufA"
                     /locus_tag="VFL49_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HET7912250.1"
                     /translation="MVAPRPRPQVMRLTDAAASRIKTVMAQAGKPVAGVRVGVKNGGC
                     AGMEYTMEYADAVNPADEIVEDKGVKLLIDPKAVLFLLGTEMDYKTEKLSAQFVFNNP
                     NQTSACGCGESVQLTPATDKNAVPSTS"
     gene            3158..3529
                     /locus_tag="VFL49_09485"
     CDS             3158..3529
                     /locus_tag="VFL49_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET7912251.1"
                     /translation="MDPGFIRELFLSFGPVTVRRMFGGAGLYAEGVMFGLVSGEQIYL
                     KADAATVAHFEREHCGPFEYSTRNGRRALTSYWRLPDRLYDDADELAQWARQAFAVAQ
                     RGVSAKSRAGKKRDRKRTRKR"
     gene            complement(3533..4603)
                     /locus_tag="VFL49_09490"
     CDS             complement(3533..4603)
                     /locus_tag="VFL49_09490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7912252.1"
                     /translation="MAGRAEEYERTIGFAEIALGQMKALRHPSNPRNYEVWYTYATGY
                     HPALNAQINATLKAKGTISEADLAQIYDSYLSPSRLTERIDEVGSQVKAEIDEVMAMI
                     DAVAGTASSYTESLAGATEQLAISKDRDGLRVIVENLVATTKAMERSNQQLEERLTAS
                     KKEINDLQENLEAVRTESLTDPLTQLANRKYFDTTLEKAIADAVKNNEPLSLMLTDVD
                     HFKAFNDSYGHLTGDQVLRLVAMSVKNNVKGKDTAARYGGEEFAVVLPNTVLRAATTV
                     ADHVRREVMSKDLMKRSTGEHLGHVTISIGVATLRKDDTQQSLIERADACLYAAKRNG
                     RNRVICETDPEVAPPNAAAQVA"
     gene            complement(4750..>6058)
                     /locus_tag="VFL49_09495"
     CDS             complement(4750..>6058)
                     /locus_tag="VFL49_09495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7912253.1"
                     /translation="SAKVLSAIEAAGYKTPTPIQDQAIPHVLARRDVLGIAQTGTGKT
                     AAFVLPMLTMLEQGRARARMPRTLILEPTRELAAQVAEHFEKYGKNHKLNVALIIGGV
                     SFDDQDAKLLRGVDVLIATPGRLLDHFERGRLLLSGCELLIIDEADRMLDMGFIPDIE
                     RIGKLVPFTRQTLFFTATMPPEIQRIADTFLHNPVRVEVAKPATTAATITQLLVATGR
                     DPHEKRETLRRLIRSADGFKNAIIFCNRKREVANLHRSLLRHKFNAVALHGDMDQPAR
                     MAALDQFRRGEVQLLIASDVAARGLDIPEVSHVLNFDVPHHPDDYVHRIGRTGRAGRS
                     GTAITIVAPIDSKAVSAIERLIGQSISWMDKPAPAKTIEVNAGEEARPPAPKPSGRHR
                     RQQAARPAPKQRDGVRVRAPQAAEEDSSGHLPAFLLRPVSVKA"
BASE COUNT         1177 a         1759 c         1945 g         1177 t
ORIGIN      
        1 gcccaaggca aagtaccggc attgccgggc ctcagtgcgg gccgggttac catcgtgaac
       61 ggccgctacg agccgacgtg gagcgatttc aagacggcgg caggagtcga gcttggcgac
      121 ttgttcgcct ttgcggcgga gcaaaaagac catcggctgg gcgatgtgcc gggccaagac
      181 gatatcgcca tgtcgctcaa tacggccttc atgacgggcg gcgtcgcgct gcgtgtggca
      241 aaaggcgccg atgtcgaaaa gccggtcgaa attgctcact tcttttccga caactcggcg
      301 gcggcgatgt acccgcgctc cgtggtgctc gtggagccgg gcgcgcggtt gacgctcatt
      361 gaatcgttca gcggccctgc cggcgtcgac taccaggtca attccgcact cgagctggtg
      421 atcggcgagg gggcgcatgt cgatcacgtc acgatcggcc atgatggaga tgcggcgctg
      481 cgcatttcca ctttgggcct gaaggtcggg gccaacgtca tctacaatgg cttcacttat
      541 acggcgggcg gcgcggtcgt gcgcaatcag atttttgcgc actttaatgg caaggaaacg
      601 caagcttcaa ttgggggggc gaccctgctg aaaggacgcc agcacgtcga caatacgctc
      661 gtcatcaatc acgcggcggg cggctgcacc agccgcgagc gcttccgcac cgtgctcgac
      721 ggagagagcc acggcatttt ccagggcaag atcatcgtcg agccgcacgc gcagaagacc
      781 gacgcgaaaa tggcaagcca tgcgctcctg ctctccgaca gcgcggaagc cgacaacaag
      841 cccgagctcg aaatcttcgc ggacgatgtg cagtgcggcc acggcgcgac ggccggcgcg
      901 ctcgatcgca ccctcaagtt ctacctgatg tcgcgcggca ttcccgaaaa tgaggccgag
      961 gccctgctga tccaggcttt cgtaggcgag gcgatcgagg aaatcgcgca cgagggaatt
     1021 cgcgaagcgc tgctcaatgc ggccgccaaa tggctggatg cccgggagca atgagaatgc
     1081 accctgccgt cagcaatggc tcatatgacg tggccaaaat ccgcgcggat tttcccgcgc
     1141 ttgcaatgaa ggtctatggt aagccgctcg tctatctcga caacgctgca tcggcgcaga
     1201 agccgaaggc cgtgctcgac aggctcgcaa aggcttacac cgagcaatac gccaacgtgc
     1261 accgcgggct gcattttctc gccaacgagg cgaccgaagc ttacgagggc gcacgcgaaa
     1321 cggtgcgtgc cttcctgaac gcggaacgca aggaagagat catcttcacg cgcaacgcca
     1381 cggaagcggt caatctcgtt gcctatactt tcggccgcga gcgtatcaag gcgggcgatg
     1441 agatcgtgct ctccatcatg gagcaccatt cgaatatcgt tccctggcac ttcctgcgcg
     1501 agcggcaggg cgccgtcatc aaatgggcgc cgatcgatga ggacggcaat ttcctgctgg
     1561 atgaattcga aaagctgctg acgccccgca ccaggatggt ggcggtcacg cacatgtcga
     1621 atgcgctcgg caccgccgtc cccgtgaagg aggtcgtgcg catcgcgcac gcgcggggca
     1681 tcccggtgct ggtcgacggc gcgcaggcgg cggtgcacct cgacgtcgac gttcgcgaca
     1741 ttgactgcga tttctacgcc atcaccggcc acaagcttta cgggccgacc gggatcgggg
     1801 cgctgtacgg caagtacgag catcttgcgg cgatgccgcc gttcaatggc ggcggcgaaa
     1861 tgattcacga agtgttcgag gaccgcatca cttacggcga gccgccgcac aaattcgagg
     1921 cgggcacgcc ggcgatcgtg caggctatcg gctttggcgc ggccatcgat tatgtgaatt
     1981 cggtgggcaa ggcgcgtatc cgcgcgcatg aagatgcgtt ggtcaaatac gcccacgagc
     2041 agctcggcaa gatcaactca ttgcggatta tcggcagggc gcctgacaaa ggtccgatcg
     2101 tatcgtttga aatgaaaggc gcgcatcctc acgatgtcgc aacgatcata gaccgggccg
     2161 gcgtggcggt tcgcgccggc acccactgcg tgatgccttt gctcgcgcgc tacggattga
     2221 ccgcgacctg ccgtgcctcg ctggcgatgt acaacaccag ggacgaggtc gatgctctcg
     2281 tcgcttcgct tgtgaaagct cgggaatttt ttacatgacg gacgatacga cccagaatgc
     2341 gccagttgcg gaaaagccgg cggagaatgc ggcagtcacc ggttcttcgg cattgccgca
     2401 ggatgaactc aacaagcgga ccgacgacat cgtcgcggcg ctgaaaaccg tttacgaccc
     2461 cgagattccc gccgacatct atgagctggg tctgatctac aggatcgata tcggcgacga
     2521 tcggggtgtg aagatcgata tgtcattgac gtcgccgaac tgcccggcgg cgcaggacct
     2581 gccgatcatg gtcgagaacg ccgtgtcgag cgtgccgggg gtgaaggaga ccaaggtgga
     2641 ggtcgtttgg gacccgccgt gggatccgag ccggatgtcg gacgaagccc ggcttgtgct
     2701 caacatgtgg taatctgcga acgcgcgact acatagagat tcgcgaagag gatgaaatgg
     2761 ttgctccgag accaagaccg caggtgatgc ggctgaccga cgccgcagcg tcgcgcatca
     2821 agaccgtgat ggcgcaggcg ggcaaacccg tggccggcgt gcgggtcggc gtaaaaaacg
     2881 gcggctgcgc gggcatggaa tatacgatgg agtatgccga cgccgtcaac ccggcagacg
     2941 agattgttga ggataagggc gtcaagcttc tgatcgatcc gaaagcagtg ctgttccttc
     3001 tcggaacgga aatggattac aagaccgaaa agctgtcggc ccagttcgtg ttcaacaatc
     3061 cgaaccagac cagcgcgtgc gggtgcggag aatctgtgca gctcacgccg gccaccgaca
     3121 aaaacgccgt cccgtcaacg tcataggcgg ccgcggcgtg gatcccgggt tcatccgcga
     3181 gctgttcctg tctttcggcc cggtcactgt gcgtcgtatg ttcggcggcg ccggccttta
     3241 cgccgaaggt gtgatgtttg gccttgtgtc cggcgagcag atttatctca aggccgatgc
     3301 ggcgacggtg gcacactttg aacgtgagca ctgcgggcct ttcgaatatt cgacgaggaa
     3361 cggcaggcgt gcgctgactt catattggcg gctgcccgat cggctctacg acgacgccga
     3421 tgaacttgcg caatgggcgc gacaagcgtt tgcggttgcg cagcggggcg tatcggcaaa
     3481 atccagggcc ggaaagaagc gcgacaggaa acgcaccagg aaacgttgag gattatgcaa
     3541 cttgtgcagc ggcgttcggc ggggcgactt ccggatccgt ctcgcaaata acgcggttgc
     3601 ggccgttgcg cttggcggca taaaggcacg cgtcggcgcg ttcgatcagc gattgctgcg
     3661 tgtcgtcctt gcgcaaggtg gcgaccccga ttgaaatcgt cacgtggccg aggtgctcgc
     3721 cggtcgagcg cttcatcaaa tccttggaca tcacttcacg ccggacgtga tcggcgaccg
     3781 tagtcgctgc ccgcaagacg gtatttggca gaacgacggc gaactcttcg ccaccgtagc
     3841 gtgccgccgt gtccttgcct ttgacgttat ttttcacaga catcgcaaca aggcgcagga
     3901 cctgatcgcc ggtcagatga ccatagctgt cgttgaaagc cttgaagtga tcgacgtccg
     3961 tcagcatcag ggataacggc tcgttgtttt taactgcgtc agcgatggct ttctccagcg
     4021 tggtgtcgaa atatttccga ttggcgagtt gggtgagggg atcggtcagg ctttcggtgc
     4081 gaacggcctc caggttctcc tgaagatcat tgatttcctt cttggaagcc gtcagccgct
     4141 cttccaattg ctgattcgag cgctccatgg ccttggtcgt tgccacgaga ttttcgacga
     4201 tgacccgcag cccgtcgcga tcctttgaaa tcgcaagctg ttcagtcgcg ccggcgaggc
     4261 tttcggtata gctgcttgcg gtgccggcaa ctgcatcgat catggccatg acctcgtcga
     4321 tctcggcctt gacctggctg cccacctcgt cgatgcgctc ggtcagccgc gacggcgaaa
     4381 gatagctgtc atagatttgg gcgagatccg cttcactgat cgtacccttg gccttgagcg
     4441 ttgcgttaat ctgcgcattc agcgccggat gatagcccgt cgcgtaagtg taccagactt
     4501 cgtaattgcg cggattgctt gggtgccgca gcgccttcat ctgcccgaga gcaatctcgg
     4561 caaatccaat tgtgcgttcg tactcttcgg cacgcccggc cattgctaag acccccgaat
     4621 gcaggcttgg tcctgcagag gacggcacgc aaggcgtctc tcttgggccg gacggtatgc
     4681 gacggaaatg aatgaccggt aaattcggaa cccgtgcgct gcggcaagcg cacgaacgac
     4741 ccgcagcaat caggctttga ctgaaaccgg ccgcagcagg aaggccggca aatggccgct
     4801 gctgtcttct tcagccgcct gcggcgccct gacccgaact ccatcccgtt gcttcggcgc
     4861 cggtcgggcg gcttgctggc gccggtgacg gcctgatggc ttgggtgccg gcggccgcgc
     4921 ttcttcccct gcgtttactt caatcgtctt ggcaggcgcc ggcttgtcca tccatgagat
     4981 cgattggccg atcaaccgct cgatcgcgct cacagccttg ctgtcgatcg gcgccacgat
     5041 cgtgattgcg gtgccgcttc gtccggcgcg gccggtacgg ccgatgcgat ggacgtaatc
     5101 gtcggggtga tgcggcacgt cgaagttgag aacgtgactc acttccggaa tgtcgaggcc
     5161 gcgggcggcg acgtccgagg caatgagcaa ttgcacttcg ccgcgccgaa actgatcaag
     5221 cgccgccatg cgtgcaggct gatccatatc gccgtgcagg gcaaccgcat tgaacttgtg
     5281 ccggagcagc gagcgatgaa gattggcgac ttcacgcttg cggttgcaga agatgatggc
     5341 gttcttgaag ccgtccgcgg agcggatcag ccggcgcaga gtctcgcgtt tctcgtgcgg
     5401 atcgcggccg gtcgcgacca gcaattgggt gatcgttgcg gcggtggtgg cgggcttggc
     5461 cacttcgacg cgcaccggat tatgcaggaa ggtatctgcg atgcgctgga tttccggagg
     5521 catcgtggcg gtgaaaaaca gtgtttgccg ggtgaacggc acgagctttc cgatgcgctc
     5581 gatgtcggga atgaaaccca tgtcgagcat acggtcggcc tcgtcgatga tcaggagttc
     5641 gcatcccgac agcagcagcc gtccgcgttc gaagtggtcg agcaaccggc caggcgtggc
     5701 gatcaacacg tcaacgccgc gcagcaattt ggcgtcctgg tcgtcgaatg acacgccgcc
     5761 gatgatcagc gcaacgttga gcttatggtt cttgccgtat ttttcaaagt gttcggcaac
     5821 ctgggcggca agctcgcgcg tcggttcaag aatgagagtg cgcggcatgc gcgcgcgtgc
     5881 gcggccctgt tcgagcattg taagcatcgg cagcacgaag gcggcggtct tgccggtacc
     5941 ggtttgcgca atacccaaaa cgtcgcgccg cgccaatacg tgcgggatgg cctgatcctg
     6001 gataggtgtg ggggtcttgt agccggctgc ttcgatagcc gagagaactt tggcagat
//
LOCUS       DASSRL010000466         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_362063, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000466 DASSRL010000000
VERSION     DASSRL010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362063"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFL49_09500"
     CDS             <1..710
                     /locus_tag="VFL49_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912254.1"
                     /translation="TEPKPKIVLVSDFTLASDVVAVDRGYTARLERKIGTYPAHERRQ
                     RTTERVNDEIVATIVAGLREAGLEAQPGSEDAVTLDQSAFVVSGNLRPAEPVTAKNKN
                     SFGFGAGRGHVVATVSASMFSAGSKRQVLTFDAESTAAKREPAVPPKVAAGRNAEIAA
                     ILASTGSPNERLSPDVEVQARRLGRAIADRVLAYARDKGWLAPAGQGIAGASPPRSDA
                     DNETEGSPASVSEAPKT"
     gene            complement(713..1558)
                     /locus_tag="VFL49_09505"
     CDS             complement(713..1558)
                     /locus_tag="VFL49_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7912255.1"
                     /translation="MRFEGTKAYVATDDLKIAVNAAITLERPLLVKGEPGTGKTVLAL
                     EVARALGAPLIEWHIKSTTKALQGLYEYDAVSRLRDSQLGDERVKDIRNYIKRGKLWD
                     AFVAEERPVLLIDEIDKADIEFPNDLLQELDRMEFFVYETGETVKARRRPIVVITSNN
                     EKELPDAFLRRCFFHYIRFPDTETMRAIIEVHFPGIKQRLVAEALRLFYEIRDVPGMK
                     KKPSTSELLDWLKLLMAEDIGAETLRERDPKKLIPPLHGALIKNEQDVHLFERLAFMA
                     RREAR"
     gene            complement(1635..>3429)
                     /locus_tag="VFL49_09510"
     CDS             complement(1635..>3429)
                     /locus_tag="VFL49_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7912256.1"
                     /translation="ADAHYNLGSSLRVLGRYDEALTSLDRALALQPDFTKALNNRAAV
                     LEGLNRLPEALADYERAIALNPAFTEARNNRGRVLLGLDRADDAIDNFTAALKINPRD
                     AEAWYQRGRLLLEISRYEEAAADIRQALTIEPGHAEARLASCFAELPVIYKDEAEISQ
                     RRAAYEHKLRALGEDVERGALKGDLIKAIGSRHPFLLAYQGGDDRALQQLYGDIVNRI
                     VAQRFPPAPLPPPPAPGEVIRVGVVSSFFYRHSNWKIPIKGWVGQLDRKRFHVTGYHL
                     GAIRDEQTEAASRLCDRFVHRTLDTDGWRREILADAPHILIYPGLLMDISSIQLAAQR
                     LARVQCNSWGHPETSGLPTMDYFLSSDLMEPPEADAFYTEKLIRLPNLSIYYEPVNAE
                     PVEVTREELGLRASAIAFWSGQSLFKYLPQYDDVFVRIAREVSDCQFVFLRHFGAPKI
                     TALIRERLDRAFAAAGMRADDHCVFLNRLSQSKFVAAAGLSDIFLDSIGWSGCNSALE
                     CLDQALPVVTFEGKTMRGRHCAAILRMLDVPETIATTIDDYVAIAARLGRDRQFRQAI
                     SSRMAENRHKVYRDCAPVTALEDFIERCVRG"
BASE COUNT          647 a          993 c         1109 g          680 t
ORIGIN      
        1 cgacggaacc gaaaccgaaa attgttttgg tttctgattt caccttggct tctgatgtgg
       61 tcgcggtcga tcgcggctat accgcgcgtc ttgaacgaaa gatcggcaca tatccggcgc
      121 atgaacgccg gcagcgcacg accgagcgcg tcaacgatga gatcgtcgcc accattgttg
      181 caggtcttcg ggaggccggg cttgaggcgc agccaggttc ggaagatgcc gtcacgctcg
      241 atcagagcgc ctttgttgtg tcggggaact tgcgcccggc cgaaccggtt actgccaaga
      301 acaaaaacag cttcggtttc ggggccgggc gagggcacgt ggtcgcaacc gtcagcgcat
      361 cgatgttttc cgccggaagc aagcgccaag tgttgacctt tgatgctgag tcgactgcag
      421 ccaaacgcga gccagcggtg ccgccgaagg tcgcggcagg ccgcaacgcg gagatcgctg
      481 ccattctggc gtccacggga agtccgaatg aacggttgtc tcccgacgtg gaagttcagg
      541 cacggcgtct cgggcgcgcg atcgccgacc gggtcctggc ttatgccagg gataagggct
      601 ggctggcgcc ggcggggcag gggatagccg gggcgtcgcc gccgagaagc gatgccgaca
      661 atgaaaccga gggctccccg gcgtctgttt ccgaggcgcc gaagacctga ctttagcggg
      721 cttctcgccg cgccatgaat gcaagccgct cgaacaggtg cacatcctgc tcgttcttga
      781 tcagcgcccc atgcaacggc gggataagct ttttggggtc gcgctcgcgc agtgtctccg
      841 ctccgatatc ctcggccatc agaagcttga gccagtcgag gagctcggac gtcgacggct
      901 tctttttcat gccaggaaca tcgcggatct cgtaaaacag tctcaaggct tccgcgacga
      961 gcctctgctt gatgccgggg aaatggactt cgataatcgc gcgcatcgtt tccgtgtcgg
     1021 gaaagcggat gtagtgaaag aagcatcgcc gcaaaaaagc gtcgggtaat tctttttcat
     1081 tgttcgaggt gatgaccacg atcggccggc gccgcgcctt gacggtttcg ccggtttcgt
     1141 agacgaaaaa ttccatgcga tcgagttcct gtaacaggtc gttcggaaac tcgatgtcgg
     1201 ccttgtcgat ctcatcgatc aggagaacag ggcgctcttc tgcaacgaat gcgtcccaga
     1261 gtttgccgcg tttgatgtag ttgcggatgt ccttgacgcg ctcgtcacca agttggctgt
     1321 cgcgcagccg cgacaccgcg tcgtactcgt agaggccctg cagcgccttg gtggtggatt
     1381 tgatgtgcca ttcgatcaga ggggcgccga gcgccctggc gacttcgagc gccagcacgg
     1441 tcttgccggt tccgggttcg cctttgacca gcagaggacg ctcgagcgtg attgcggcat
     1501 tgaccgcgat tttgaggtcg tcggttgcca cataggcttt ggtaccttcg aagcgcatag
     1561 aacccgttcc ctttgccggc cgcctcattc atatagggaa gcggcagcgg gacaaagaga
     1621 tttggccagg cggtctagcc gcgcacacac cgttcgatga agtcttccag tgccgtcacc
     1681 ggcgcgcaat cgcgatagac tttatgccgg ttttcggcca ttcgtgacga gatcgcctgg
     1741 cggaattgcc tgtcccggcc gagccgcgcc gcaattgcaa cgtagtcgtc tatcgtcgtg
     1801 gcgattgtct ccggaacatc cagcatccgc agtattgccg cgcaatggcg gccgcgcatt
     1861 gtttttccct cgaacgtgac gaccggcagc gcctggtcaa gacattccag ggccgagttg
     1921 cagcccgacc agccgatgct gtccaggaat atgtcggaga gaccggccgc ggccacgaat
     1981 ttgctttgac tcaaccgatt aaggaaaacg caatggtcgt ccgcgcgcat gccggctgcg
     2041 gcaaaagccc ggtccaggcg ctcgcgaatg agcgcagtga tcttcggcgc cccgaaatgg
     2101 cgcaggaaca cgaactgaca atcactgacc tcacgcgcga tccgcacgaa tacgtcgtcg
     2161 tattgcggca ggtacttgaa gagagactgg ccgctccaga acgcgatcgc gcttgcgcgc
     2221 agaccgagct cctcgcgcgt aacctcgacc ggctcggcgt tgacgggttc gtagtagatc
     2281 gacagattgg gcaggcgaat gagcttttcg gtgtagaaag cgtctgcctc gggcggctcc
     2341 atcagatcgc tcgacaggaa gtagtccatt gtcgggagcc cgctggtttc cggatgaccc
     2401 caggaattgc actgcacgcg ggcaagacgc tgcgccgcca gctgtatcga cgaaatgtcc
     2461 atgagcagtc ccggatagat caggatgtgc ggagcgtcgg cgagaatttc gcggcgccag
     2521 ccatccgtat ccagcgtgcg gtggacgaaa cggtcgcaca ggcgcgaggc ggcttcggtc
     2581 tgctcgtcgc ggatggcgcc cagatgataa ccggtgacgt gaaagcgctt gcggtcaagc
     2641 tggcctaccc agcccttgat cggaattttc cagttcgaat ggcggtagaa aaagctgctg
     2701 acgacgccga cacggatcac ttctccaggt gcgggtggcg gaggtagcgg tgcgggcgga
     2761 aagcgttgcg cgacgatccg gttgacgata tcgccataaa gctgctgcaa cgcccggtcg
     2821 tcgccgccct gataggcgag caggaaagga tgccgggagc cgatcgcctt gatcaggtcg
     2881 cctttgagcg ccccgcgttc gacgtcctcg ccgagtgcac gcagcttgtg ttcgtaggcg
     2941 gcacggcgct gcgagatttc ggcttcatct ttgtaaatga ccggcagttc ggcaaaacat
     3001 gatgcaaggc gtgcttccgc gtgcccaggc tcgattgtca aggcttgcct gatatccgct
     3061 gccgcttcct cgtagcggct gatctccagc agcagccggc cgcgttgata ccaggcttcg
     3121 gcatcgcgcg gattgatctt cagggcagct gtaaaattgt cgatcgcatc gtcggcacgg
     3181 tcgaggccga gcagcacgcg gccgcgattg tttcgggcct cggtgaaagc ggggttgagc
     3241 gcgatggcgc gctcgtaatc ggcaagcgct tcgggtaggc gattgagccc ctccaggacg
     3301 gcagcgcggt tgttcagcgc cttggtgaag tcgggctgca aggcgagggc gcggtcgaga
     3361 cttgtcaggg cctcgtcata gcggccgagc acgcgcagcg acgagccgag gttgtagtgg
     3421 gcatcggca
//
LOCUS       DASSRL010000467         5619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_939779, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000467 DASSRL010000000
VERSION     DASSRL010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939779"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..697
                     /locus_tag="VFL49_09515"
     CDS             152..697
                     /locus_tag="VFL49_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HET7912257.1"
                     /translation="MDKRLNNLVAVTVVILSVFMAISKIKDDNVTQAMQKAKQESVDA
                     WAEYQASRIKLHVDENALTQLRALESAGNIDRAISARQAADYEADIKKYEARSKETRA
                     KAEALEREYDRLNFRDDQFDMSDAFLSIAIAVAAVAALADSFWLLYVAWASGAVGFAF
                     GVAGFLGINFRPDWLAQLLGT"
     gene            798..1064
                     /locus_tag="VFL49_09520"
     CDS             798..1064
                     /locus_tag="VFL49_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912258.1"
                     /translation="MLTKSKFTGAALAILALVGCLVATSGNAQAHPRFGVGLGIGIAT
                     GAVIGAAVASGTYASPVYGYRECRYVERYDRWGNIRLIKVCDVY"
     gene            complement(1160..2539)
                     /locus_tag="VFL49_09525"
     CDS             complement(1160..2539)
                     /locus_tag="VFL49_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HET7912259.1"
                     /translation="MTLRVRHVIAVVALAAGGHLPPALAQSGGQEGPNSNGITEPSVA
                     TSLPQNGDPYGSRKHLYDHGVSYNVIYTNDVLGNMRGGIRRGTIDQGKFEGQLSVDLE
                     KLAGWKDWTFYANAFGIYNSGRIRRDYVGGMNTIAAIEATPTVRLSELWLERKIFDGA
                     ASLRFGQLAADTEFFFSNLSAMFLQSDWPTIAAVNLPGGGPAYPLSTPGVRLKIDFNK
                     DASFLAAVFNGNPAGPCAGDPDTCNRYGLNFRLRDPAFFVGELQFRSNQGKEDTGLAR
                     TIKVGGWSHLGQFPDKRFDNTGVSLASPASSGVPLMHRGDFGIYGVIDQQLYRPPGGD
                     AESGISIFNRSSISPSDRNLVNVEVDVGIVFAGMIPKRPDDRFGASFIYSRFSDSVRG
                     FDQDQINFGNLLTPPRDYEANLEFTYVAQIVPGWIVQPVYTLIWHPSGTGVRYPDAQV
                     AGFRSIIRF"
     gene            2762..4042
                     /locus_tag="VFL49_09530"
     CDS             2762..4042
                     /locus_tag="VFL49_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7912260.1"
                     /translation="MNRVVKGIVRQLTLRRAGAGHWLFAGVLLLSCAWLAVAATTRAP
                     GGDRIDPKDVPAKGQQDGSAYRPTPKQWASLGISPVAEVVFRTEHMTEGKIAVDEDRA
                     TLVFSPYSGRVIKLLAKPGEAVKAGQPLFVVEAPDMVQAQNDFINSISGLNKARSALD
                     LARIVEQQNKTLFEGRAGSLRDLQQSQASTRAGENDVRAAQTTVEVSRNRLRILGMTD
                     EEITRFSDTGAVSPHMTIYSPLAGTVIQRKVGPGQYINTSSQNTSASDPTFVIGDLST
                     VWLVAYVRESEAPNVHVGQAVNFKVLAYPNRTFAANISYVATSLDTGTRRLLVRATID
                     NSQNLLRPEMFANVKILTTEGDSSLGVPREAIVYDGKSTHVWIVRNDQSVERREVKTG
                     LSNGQMVEVVDGLRHGESVISKGTLFVDRAAAGS"
     gene            4050..>5619
                     /locus_tag="VFL49_09535"
     CDS             4050..>5619
                     /locus_tag="VFL49_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7912261.1"
                     /translation="MLRSLMSFCLSRRLLVLVAYVAFLGIGFATLKALNVEAYPDPAP
                     PIIEIIAQYPGQSPEEVERYVTIPLEIAVASTPGLTYIRSNTVFALGFIRLQFEYGRD
                     YNFVRQQVTNRLKDVTLPLTVTPVISPAGGISEILRYQLKGPPGTDLIQLKTIQDWVV
                     ERKLRIVPGVADVAVLGGKTKEYQAEIDLDRMRAYGLTLPQIINAISTSNSNVGGRTI
                     TIGEQSVNVRGVGALQSVNDIDNIVISQQGGLPVILKDVARNQIGFTPRLGIAARDSQ
                     DDIIFGVVLMQKLERTMDVVTRVRAAVERINADGSLPPGVKIEPFYDRGDLVAITVRT
                     VMHNMLFGIALIFLIQWVFLGNFRCALIVAATIPVALFLAVIITVLRGESANLLSVGA
                     IDLGIIVDATVIMVENIYRHLADHARRAIADPQARLADKLHRILVAAVEVDKPIFFSV
                     IITIAAFLPLFTMQGVEGQIFGPMSRAYAYALLGAVIATFTVTPVMSSILLPDKVNEV
                     ETFIVRGIRKVYEPV"
BASE COUNT         1137 a         1720 c         1618 g         1144 t
ORIGIN      
        1 ggccatccaa acgaatttca cggctcgcct tctattcacg cgactagcca tagcccctga
       61 ccgccattgc agcaaatccc gcaaccttcc gctaaagtgg acttgctgcg gaccgggggg
      121 acagatggac gcggatgtca gaaccgacag cttggacaag cgactcaaca atctggtggc
      181 tgtgacggtc gttattcttt cggtcttcat ggcgatctcg aagatcaagg atgacaatgt
      241 cacccaggcc atgcagaagg ccaagcagga gtccgtcgac gcctgggccg aatatcaggc
      301 ctcccgcatc aagctgcatg tcgacgagaa tgcgttgacg caactgcggg ctctcgaaag
      361 cgcgggcaac atcgaccgtg cgatctccgc tcgccaggcg gcggattacg aggccgacat
      421 caagaagtat gaggcgcgct ccaaagaaac ccgcgcaaag gccgaggcgc tggaaaggga
      481 atatgaccgc ctcaactttc gcgacgacca gttcgacatg tccgacgcgt ttctttcgat
      541 cgcgatcgcg gtcgcggcgg tagcggcgct ggctgacagt ttctggcttc tgtacgtcgc
      601 ctgggcttca ggcgccgtcg gctttgcttt cggcgtggcc ggatttctgg gcatcaattt
      661 ccgcccggat tggctggcgc agctgttagg cacctgaaat tgcatgcatt ttagtgcttt
      721 gaacctttcg cgctccatcc acgaccaatc ggtatgcggc cgacctggcc gcccccgatt
      781 caaggatgga gagagccatg ttgaccaagt ccaaattcac cggtgccgcg cttgcgatcc
      841 ttgcgctcgt tggctgcctc gttgcaacga gcggtaacgc ccaagcccat ccccgctttg
      901 gtgtcggtct tggcatcggc attgccaccg gcgcggtgat cggcgccgcc gtcgcgtccg
      961 gcacctatgc gagcccggtc tacggctacc gtgaatgccg ctacgtcgag cgctacgacc
     1021 gctggggcaa cattcgcctg atcaaggttt gcgacgtcta ctgaccgcaa ggttgagatc
     1081 ggcgcctgcc tcccgtccat cctcgcgccg atcccacacg acccgcccgg cattgtcccc
     1141 gtccgggcgg gtcacttttt cagaacctga tgatcgaccg gaatccggcg acctgcgcat
     1201 cgggatagcg aacacctgtc ccgcttggat gccaaatgag cgtatagacc ggctgcacga
     1261 tccagccggg gacgatctgt gccacgtagg tgaactcgag gttggcttcg tagtcgcgcg
     1321 gcggagtgag gagattcccg aaattgatct gatcctggtc gaaaccgcgg acgctgtctg
     1381 aaaaccgcga gtagataaaa cttgctccaa agcggtcgtc gggccgtttg ggaatcatgc
     1441 cggcaaacac gattccaacg tcgacctcca cgttgacgag attgcggtct gacgggctga
     1501 ttgatgaacg attgaatatc gagattccgc tttcagcgtc accgcccggc ggccgataaa
     1561 gctgctgatc gatgaccccg taaattccga aatcgccgcg atgcatgagc ggcacgccgc
     1621 tgctcgcagg acttgccaac gacacccccg tgttgtcgaa gcgtttgtcg ggaaattgcc
     1681 caaggtgact ccagcctccg accttgatcg tgcgcgcgag tccggtgtcc tcctttcctt
     1741 gattgctgcg gaactgcagt tccccgacga aaaacgccgg atcgcgcagg cgaaaattca
     1801 agccataccg attacaggtg tccggatcac ccgcacaggg acccgccgga ttgccattaa
     1861 agaccgcggc aagaaacgac gcatccttgt tgaagtcgat cttcagccgc acgcccggtg
     1921 tcgacagcgg ataagcaggt ccgccgccgg gcagattgac ggcggcaatc gtaggccagt
     1981 cgctttgcaa aaacatcgcg ctcaagttac tgaagaaaaa ttcagtatca gctgcgagct
     2041 ggccgaaccg cagacttgct gctccatcaa aaatttttcg ctcgagccac aactccgaca
     2101 agcgcactgt cggcgttgct tcaatggcgg cgatcgtatt catgccgccg acatagtcgc
     2161 ggcgaatgcg gccggagttg taaatcccaa aagcattcgc gtagaacgtc cagtctttcc
     2221 atccggccag cttctcaaga tcgaccgata attgcccttc gaacttgccc tggtcgattg
     2281 tcccgcgacg gatgccgccg cgcatattgc ccagaacgtc gttcgtgtag atgacgttgt
     2341 acgagacacc gtgatcataa aggtgtttgc gagatccgta cggatcgccg ttctgcggca
     2401 ggctggtcgc gaccgatggc tcggtaatcc cgttgctgtt cggcccttct tgcccgccgg
     2461 actgagccag cgccggcgga aggtgaccgc cggcggcaag agcaacaacc gcaatcacat
     2521 gccgaaccct taacgtcata cgccagcgtc gttttcgtgt ttgaccacag gaagcgccag
     2581 cataaccggc ctgttgcgat tcatttgcaa taaaatcatg cacaagcgcc gtagccaacc
     2641 ggcggcaagg ggttgaatct ccggcattta ccgactgcga attctttatt tcacggtaat
     2701 gccgtagcgc ggcgtccatt cactataagc cttgcaacag ggacagggtg ggatcggttt
     2761 cttgaatcgc gtcgtcaaag gcattgtgcg gcagctgacg cttcggcgcg cgggtgcggg
     2821 tcattggcta ttcgccgggg ttctcctgct cagttgtgcc tggcttgccg tcgccgcgac
     2881 gacgcgtgct ccgggcggcg acagaattga tcctaaggac gtgccggcaa aaggtcagca
     2941 ggacggatct gcataccgtc ctacgccgaa gcaatgggcg tcgctcggga tctctccggt
     3001 cgccgaagtc gtgttccgca ccgagcacat gacggaaggc aagatcgctg tcgacgagga
     3061 ccgtgcaacg ctcgtgttct ctccgtattc aggtcgcgtg atcaaacttc ttgcaaaacc
     3121 tggcgaggcg gtaaaggccg gacagcctct cttcgtcgtc gaagcgccgg acatggttca
     3181 ggcgcagaac gatttcatca attccatctc cggcctgaac aaggcccggt cggctttgga
     3241 cctcgcccga atcgtcgaac agcagaacaa gacgttgttt gaaggtcgcg ccggttcgct
     3301 tcgcgatttg cagcaatcgc aagcaagcac gcgcgccggc gaaaacgacg tgcgagccgc
     3361 ccagaccacg gtcgaggtga gccgcaatcg cctgcgcatc cttggcatga ccgacgagga
     3421 gatcacgaga ttcagcgaca cgggtgcggt cagcccgcat atgacgattt attcgccgct
     3481 tgccggtact gtgatccagc gcaaagtcgg cccggggcaa tatatcaaca cgtcatcgca
     3541 gaacaccagc gccagcgatc cgacgttcgt catcggcgat ctttcgaccg tctggctcgt
     3601 cgcttacgtg cgcgagtccg aggcgcccaa cgtccacgtg ggccaagcgg ttaacttcaa
     3661 ggtgcttgcc tatccgaacc ggactttcgc ggcgaatatt tcatacgtcg cgacatctct
     3721 cgacaccggc acgcggcggc ttctggtgcg cgctacaatc gacaattcac agaacctgct
     3781 gcgccccgag atgttcgcta acgtgaaaat cctcaccacc gagggcgaca gctcgctcgg
     3841 cgtgccgcgc gaagcgatcg tctacgacgg gaaatcgacc cacgtctgga tcgtccggaa
     3901 tgatcaatcc gtcgaacggc gggaagtaaa gacaggacta tccaacggcc agatggtcga
     3961 agtcgtcgat ggattgcgcc acggcgagag tgtcataagc aagggcacat tgttcgtcga
     4021 tcgcgcagca gccggaagct gacccttcga tgttgcgtag cctgatgtcg ttctgcctgt
     4081 cgcggcggct tctcgttctg gtcgcctacg tcgcatttct cggaatcggc ttcgcgaccc
     4141 tcaaggcgct caatgtcgag gcctatccag accctgcgcc gccaatcatc gaaatcatcg
     4201 cgcagtatcc gggccagtcg ccagaagaag tcgagcgcta cgtcacgatt ccgctcgaga
     4261 tcgcggtcgc gagcacgccc ggcctcacct atatccgttc gaatacggtt ttcgccctcg
     4321 gttttatccg cttgcagttc gagtatggcc gcgactacaa tttcgtgcgc cagcaggtca
     4381 ccaaccggtt gaaagatgtg acgttgccgc tgaccgtgac gccggtgatt tcgccggctg
     4441 gcggcatcag tgaaattctg cgctaccagc tcaagggtcc gccggggacg gacctgatcc
     4501 agctgaagac aatccaggac tgggtggtcg agcgcaaatt gcgcatcgtt ccgggggtgg
     4561 cggacgtcgc cgtcctcggc ggaaaaacca aggaatatca ggccgaaatt gatctcgatc
     4621 gcatgcgggc ctacgggctc actttgcccc agatcatcaa tgcgatctcg accagcaatt
     4681 cgaacgtcgg cggacgcacc atcacgatcg gggagcaatc ggtcaatgtc cgcggagtcg
     4741 gcgcgctgca atcggtcaat gacatcgaca acatcgtcat aagccagcag ggcggccttc
     4801 cggttattct gaaggatgtg gcgcgtaacc agatcggatt cacgccgcgc ctcggaatcg
     4861 ccgcccgcga cagccaggac gacatcatct tcggcgtcgt cctgatgcaa aagctcgaac
     4921 gcacgatgga cgtggtgacc cgcgtacgcg cagccgttga acgcatcaac gcagacggca
     4981 gcctgcctcc cggcgtcaag atcgagccct tctacgatcg cggcgacctg gttgcgatca
     5041 ccgtacgcac ggtgatgcac aacatgctgt tcggcatcgc gctcatcttt ctgatccaat
     5101 gggtcttcct tggcaatttc cgttgcgcgc tgatcgttgc ggcgaccatt cccgtcgccc
     5161 tgtttctggc cgtcatcatc accgtgttgc gcggggagtc agccaacctc ctgtcggtcg
     5221 gggcgatcga tcttggcatc atcgtcgatg ccactgtcat catggttgag aacatctatc
     5281 gccatctggc cgaccatgcg cgccgcgcga tagccgaccc gcaggcccgg cttgccgata
     5341 agctgcatcg tattctcgtc gccgccgtcg aggtcgacaa accgatattt ttctcggtga
     5401 tcatcaccat tgcggcgttc ctaccgcttt tcacgatgca gggtgtcgaa ggccagatct
     5461 tcggaccgat gtcgcgggcc tacgcctatg cgctgcttgg tgccgtgatc gcaacgttca
     5521 cagtgacgcc ggtgatgtcg tcgattttgc tgccggacaa agtcaacgaa gtcgagacat
     5581 tcatcgtgcg cggcatccgc aaagtttacg agcccgtgc
//
LOCUS       DASSRL010000468         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1727096, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000468 DASSRL010000000
VERSION     DASSRL010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727096"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFL49_09540"
     CDS             complement(<1..311)
                     /locus_tag="VFL49_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HET7912262.1"
                     /translation="MSEKLSGQAQPVPFGERLASSQAFAELFREGMALVEETATYLDG
                     PGRQQSKKLERSAALAYATESMRLTTRLMQLASWLLLHRAVKEGEMSLAQAGKEKSKV
                     KL"
     gene            complement(686..880)
                     /locus_tag="VFL49_09545"
     CDS             complement(686..880)
                     /locus_tag="VFL49_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HET7912263.1"
                     /translation="MPAIDDDDKPKKKIAHEIGQDLTLLSVEELGARVQLLRDEIQRI
                     EADMTKKRASRTAADQFFKR"
     gene            983..1978
                     /locus_tag="VFL49_09550"
     CDS             983..1978
                     /locus_tag="VFL49_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET7912264.1"
                     /translation="MDALPSQMTAIGIKAAGGPDMLVPEQRPVPAPGAGEVLVKVAAA
                     GVNRPDVMQRMGLYPPPPGAPDIPGLEIAGDIVALGSGIKRWKIGDRIMALVSGGGYA
                     EYCIAHESHALPISALSMTEGAAIPETFFTVWHNAFERGGLKNGETLLVHGGSSGIGT
                     TAIQLAKAFGARVITTAGSPEKCDACRKLGADVAVNYRTEDFVAATKAATNDKGAEVI
                     MDMVGGDYIARNYEAAAIEGRIVQIAFQGSPKATVDFRRIMLKRLHHTGSTLRSRSIA
                     DKGAIARAVEENVLPLIAAGKVKPVIYKTFPLIDAPSAHALMETSTHIGKIVLTL"
     gene            2156..>3676
                     /locus_tag="VFL49_09555"
     CDS             2156..>3676
                     /locus_tag="VFL49_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7912265.1"
                     /translation="MAFAVSLFAVSTARAVDAVNVRLDAAAIDLTAATEIQRTEGDRI
                     QVSTAPGPDGIVRRIEVRAREGNNNWAVLALTNNSDEQIDRLIVVPHYRMVGSGIFWP
                     DLGLSRIAAITPSTGDRPVRQEGTTADVFRITLDPGTVITLVAELRTDKLPQIYLWEP
                     DAYKDKINSFTLYYGIVIGIAGLLALFLTILFVVKGSIMFPAAAALGWAVLIYIGIDF
                     GFWGKVFDMSAGAERVWRACGEAILSATLLVFLFAYLNLSRWHVRYAHITIAWLAALA
                     ALIAVAVFDPAVAAGIARMSLFGIAVVGLALVIYLSTHGFDRAVLLIPTWLLLVVWTM
                     AAALAVLGLVTNDIIGPALLGGLVLIVMLIGFTVMQHAFAGGITHGIVSDVERRALAL
                     TGAGDLIWDWDVSADKVFTSPETEHMLGLKRGTLEGPAATWLEVLHPLDRDRFRASLD
                     GMVEQRRGRLTQDFRLRTADGHYLWFTLKARPVVGSDGEVVRLVGTLTDVTDFKNAE"
BASE COUNT          696 a         1135 c         1093 g          752 t
ORIGIN      
        1 agcttgacct tgcttttctc cttgccggcc tgcgcaagcg acatctcgcc ttccttcacg
       61 gcgcgatgca gcagcaacca ggaggcaagc tgcatcagcc gagtggtgag ccgcatgctt
      121 tctgtcgcat aagccaacgc cgcggaccgt tcgagtttct tcgactgctg gcgcccgggc
      181 ccgtcgagat atgtcgccgt ttcctcaaca agtgccatac cctcacgaaa caactcggcg
      241 aaagcctgtg agcttgcaag ccgttcgccg aacggaaccg gctgcgcctg gcccgacaat
      301 ttttccgaca tacgccacac tctcccacac cggagcggaa ccgctccgta tacgattggg
      361 taagcatgac gcgcgcatgc gggtcctgtc cagacagcga tatggttaac attgcgatat
      421 agttgctgcg cagatccgcg ccatcacgcg cccgtttcat tttgacccag attgggacaa
      481 aaaaggagcc gccgttgccg gcggctcaag gtgataacag ggaggcgtca aacagagtgg
      541 acaggagcca ctcaatgtcc atatgaagat ggacggcgct agtaaacggc cagaaagctt
      601 aacgacaact taagaacatg cgcgcaatgc gtaaatattt tctgggcgtt tccgcatggg
      661 aataccgccg catcgccgga cggcgtcatc gcttgaaaaa ctgatcggcg gcagtgcgtg
      721 acgcccgctt cttcgtcatg tcggcttcga tacgttgaat ctcgtcgcgc aggagctgca
      781 ctcgtgcacc aagttcttcg accgagagca gggtcagatc ctgcccgatc tcgtgcgcga
      841 tttttttctt cggtttgtcg tcgtcgtcga tcgccggcat tttcgtctcc tgccgttcaa
      901 atctagccgc cgtcgcagcg gttgtcacat cgccgggccg tgcctaaaca atggcttccg
      961 gtacgcaaac gaagaggaaa caatggacgc gcttccatcc cagatgactg cgatcggcat
     1021 caaggccgca ggtggtccag acatgctcgt gccagagcag cggcctgtcc cggcgcccgg
     1081 agcaggcgaa gtcctcgtga aggttgccgc tgccggagtg aatcgtccgg acgtgatgca
     1141 gcgcatggga ctctacccgc cgccgcccgg cgcgcccgat attcccggcc tcgagattgc
     1201 gggagacatc gttgcgctcg gctccggcat caagcgatgg aaaatcggcg accggatcat
     1261 ggctttggtg tcgggcggag gttatgccga gtattgcatt gcgcacgaaa gccacgcgtt
     1321 gccgatctca gctctttcga tgactgaggg cgccgccatt ccggaaacct tcttcaccgt
     1381 gtggcacaac gcattcgagc gcggaggtct gaaaaacggc gagaccctgc tcgtgcacgg
     1441 tggctcatcc ggaatcggca cgactgcaat tcagctggcg aaggcattcg gcgcgcgcgt
     1501 catcaccacc gcgggttcac cggagaaatg tgatgcttgc cgcaagctcg gagccgatgt
     1561 cgcggttaac tataggaccg aggattttgt tgcggccacc aaagccgcga cgaacgacaa
     1621 gggcgctgag gtcataatgg acatggtggg aggggactac atcgcacgca attacgaagc
     1681 ggcagcgatc gaggggcgca tcgtgcagat tgccttccag ggaagtccca aggcgacggt
     1741 ggatttccgc cgcatcatgc tcaagcggct tcaccacacc ggctcgacgc tgcgctcgcg
     1801 ttcgatcgcc gacaaaggcg cgatcgcacg cgcggtagaa gaaaacgtgc tgccgctgat
     1861 cgccgcaggc aaggtcaagc cggttatcta caagaccttt cccttgatag acgcgccttc
     1921 agcccatgcc ctcatggaaa ccagcacaca catcggcaaa atcgtgctaa cactttgacg
     1981 gaaggtccat tttttcaaaa gccgcgaaat cggcgcaatg tcggccttaa aactcaatag
     2041 ggtgcgattg tttgcagatt ttcaatgtgg gcgaggccgc ggccgcaaac cgcacaatga
     2101 tgccgtggac ggggggtccg gttcggtgat gacgcgttgc ctgacagcgc tcgcaatggc
     2161 gtttgccgtt tcgctgttcg cggtttccac ggcacgtgca gtggatgcgg tcaatgtgcg
     2221 tcttgacgcg gccgccatcg acctgaccgc agccaccgaa atccaaagaa ccgaaggcga
     2281 ccgcattcag gtttcgaccg cacccggtcc ggacgggatc gtgcgccgta tcgaagtccg
     2341 cgcgcgcgaa ggcaacaata attgggcagt acttgcgctc accaacaaca gcgacgagca
     2401 gatcgaccgg ctgatcgtcg tgccgcacta tcggatggtc ggctcgggca tcttctggcc
     2461 ggacctcggt ctttcgcgca tcgccgcgat cacaccttcg accggcgacc gtccggtgcg
     2521 ccaggaaggc acgaccgccg acgttttccg gatcacgctc gacccgggca ccgtgatcac
     2581 acttgtcgcc gaattgcgca ccgacaagct gccgcaaatt tatttgtggg agcccgacgc
     2641 ctacaaggac aagatcaatt ccttcacgtt gtactacggc attgtcatcg gcatcgccgg
     2701 cctgctggct ttgttcctca ccatcctgtt cgtagtgaaa ggctcgatca tgttcccggc
     2761 cgccgccgca ctcggctggg ccgtgctgat ctacatcgga atcgacttcg gtttctgggg
     2821 caaggtgttc gacatgtcgg ccggcgcgga acgcgtgtgg cgcgcctgcg gcgaagcgat
     2881 cctgtcggca acgctgcttg tgtttctgtt cgcctatctc aatctgtcgc gctggcatgt
     2941 ccgctacgcc cacatcacca tcgcatggct tgccgcgctc gccgcgttga tcgccgttgc
     3001 ggtttttgat ccggccgtcg cggccggtat cgcccgcatg tcgcttttcg gcattgccgt
     3061 ggttggcctt gccctcgtca tctatctctc gacgcacggt ttcgatcgtg ccgtactgct
     3121 gatcccgacc tggctgctgc tggttgtgtg gacgatggca gccgcactgg cagtgctcgg
     3181 cctcgtcacc aatgacatca tcggtccggc actgctcggc ggcctggtgc tgatcgtgat
     3241 gctgatcggc ttcaccgtta tgcagcacgc atttgccggc ggcatcacgc acggtatcgt
     3301 gtcggatgtc gagcgccgcg cgctcgcctt gaccggtgcc ggcgacctga tctgggattg
     3361 ggacgtctcc gcagacaagg tcttcaccag cccggaaacc gagcacatgc tgggcctcaa
     3421 gcgcggaacg ttggagggcc ccgcagcgac ctggctcgag gttcttcatc ctctcgaccg
     3481 tgaccgtttt cgcgcctcgc tcgacggcat ggtggagcag cggcgaggcc gccttaccca
     3541 ggattttcgg ctgcgtaccg ctgatggcca ttatctctgg ttcacgctca aggcacggcc
     3601 cgtggtcggc tccgacggcg aagtcgtgcg cctggtggga acgctcaccg acgtgaccga
     3661 tttcaagaat gcagag
//
LOCUS       DASSRL010000469         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1097409, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000469 DASSRL010000000
VERSION     DASSRL010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097409"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFL49_09560"
     CDS             <1..819
                     /locus_tag="VFL49_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912266.1"
                     /translation="PDHSPGRVEDDRAIMDCVSEALTSRGFNVELCEADAAFDSRCAN
                     IFAMCERGAVLDRLTDAQNSGSVVVNSPNAIRNTYRHRMIELFGKHHVFSPASHVVAS
                     NAKGLPPAAAVWVKRYDFHATQPSDVIYAASEAGWHEALERFARRGIPFIIAQEHVSG
                     DLVKFYGVRSEGAPAAANWFEWFYHRDKGMLGHSFEVARLRDAAFGAAAALGLEVFGG
                     DAVIQEDGKPMIIDINAWPSYARYRDRAAQAIADHLAERFERRVRIVSRQGVES"
     gene            816..>2184
                     /locus_tag="VFL49_09565"
     CDS             816..>2184
                     /locus_tag="VFL49_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7912267.1"
                     /translation="MKGAPSRNVAPAQNDDAERRAVPGPRSKAIFDREAETMAPGLQS
                     IALYSQIVVDRAKGCTIYDVDGNEYLDFIAGIAVGSVGHCHPHYVKRISEQLTRSTFG
                     SFTTETRARFLELVGGLLPEGITHIQLFSGGAEAVEAAFRLAKSVTKKFEFVGFWGGY
                     HGKTAGVIGLLGGGYRNHQGPFPPGMHLSPYANCYRCPWKLEHPSCGLACADHLRNVI
                     KNETQDEVAAIIMEPMQGTAGNVIPPDDFVLAVREIADEFDALLIADEVLTGFGRTGT
                     MWASEQSGLKPDIMTIGKGMGGGFPLSGVASSLELMSAKPFGEPSGSSSSYGGNPLAA
                     AAGLGAVEAIINDRLVENSARVGRVMLDALKKLQEKYRFIGDVRGRGLMIGVEMVADR
                     KTKAPLDKAITLALFHEALARGLVTMSYSHVIRINPPLVITEDEALRGVDILDQSFGA
                     IARQFG"
BASE COUNT          403 a          647 c          705 g          429 t
ORIGIN      
        1 ccggaccatt cgccggggcg cgtcgaagac gatcgcgcca ttatggattg cgtcagcgag
       61 gcgctgacgt cgcgcggttt caacgtcgag ctttgcgagg ccgatgccgc gtttgacagc
      121 cgctgcgcaa acatattcgc gatgtgcgag cgcggcgcgg tgcttgatcg cctcaccgat
      181 gcccaaaact cgggttcggt cgtggtgaat tcgccgaacg cgatccgcaa cacctatcgt
      241 caccggatga tcgaactttt cggcaagcat catgttttct cgccggcgag tcacgtcgtg
      301 gcgagtaacg cgaaaggact gccgccggcg gctgcggtgt gggtcaagcg ttatgatttt
      361 catgcgacgc agccttccga tgtcatttac gccgcatcgg aagcggggtg gcatgaggcc
      421 ctggagcggt ttgccaggcg tggcattccg ttcatcattg cgcaggagca cgtgtcgggc
      481 gatctggtca aattctacgg tgtgagaagc gaaggggcgc cggcggctgc caattggttc
      541 gaatggttct atcatcgtga caagggaatg ctgggccatt ccttcgaggt cgcgcgcctg
      601 cgtgacgcgg cgttcggtgc cgcggccgct ctgggactgg aagttttcgg cggcgatgcg
      661 gtcattcagg aagacggcaa accgatgatc atcgacatca atgcctggcc gagctatgcc
      721 cgctaccgcg accgcgcggc acaggccatc gcggatcatt tggcggaacg tttcgagcgg
      781 cgcgtgcgta tcgtttcccg gcaaggagtt gagtcatgaa aggtgctccg tcccgcaatg
      841 tggcgccggc gcaaaacgac gatgccgaac ggcgcgcggt gccgggaccc aggtcgaagg
      901 cgatcttcga ccgcgaagcc gaaacaatgg caccgggcct gcagtcgatc gcactctatt
      961 cgcagatcgt cgtcgatcgg gcaaagggct gcacaatcta cgatgtcgat ggcaacgaat
     1021 atctcgattt cattgccggc atcgcggtgg gcagcgttgg ccactgccat ccccactatg
     1081 tgaagcggat cagtgagcag ctcacgcgct ccaccttcgg cagcttcacg accgagaccc
     1141 gcgcccggtt tctcgagctc gtcggcggac tgctgccgga aggcatcacc catatccagc
     1201 tgttttccgg tggggcggaa gccgtcgagg cggccttccg ccttgccaaa tcggtcacga
     1261 agaaattcga gttcgtgggc ttctggggcg gttatcacgg caagacggca ggcgtgatcg
     1321 gtctgcttgg tggtggctat cgcaatcatc agggcccgtt tccgccgggg atgcatctgt
     1381 cgccctatgc gaattgctat cgctgcccgt ggaagcttga gcatccgtcc tgcggtctcg
     1441 cctgcgccga tcatctgcgc aacgtcatca agaacgagac gcaggatgaa gtcgcggcga
     1501 tcatcatgga gccgatgcaa ggcacggccg gcaatgtcat tccgcccgac gatttcgttc
     1561 tggccgtacg ggagatcgcg gacgaattcg acgcgctgct catcgccgac gaagtcctga
     1621 ccggattcgg ccgcacagga acaatgtggg cgtccgagca atccggcctc aagcccgaca
     1681 tcatgacgat cggcaaaggg atgggcggcg gctttccgct cagcggcgtc gcatcgtcgc
     1741 ttgagctgat gtccgcgaaa ccgttcggcg agccgagcgg cagctcgtcc agctacggcg
     1801 gaaatccgct ggcggccgcc gccggccttg gcgcagtcga agctatcatc aacgatcggc
     1861 ttgtcgaaaa ttcggcgcgc gtcggcaggg taatgctcga cgcgctgaag aagctgcagg
     1921 aaaaataccg cttcatcggc gatgtgcgcg ggcgcggcct gatgatcggt gtcgagatgg
     1981 tggccgatcg caagaccaag gcgcctctgg acaaggccat cacgcttgcg cttttccatg
     2041 aagcattggc gcgcggcctg gtcacgatgt cgtattcgca cgtcattcgc atcaacccgc
     2101 cattggtgat caccgaggac gaagcgttgc gcggcgtcga tattctcgac cagtccttcg
     2161 gtgccatcgc gcgtcaattc gggc
//
LOCUS       DASSRL010000470         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_519941, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000470 DASSRL010000000
VERSION     DASSRL010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519941"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFL49_09570"
     CDS             <1..720
                     /locus_tag="VFL49_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7912268.1"
                     /translation="ERLGANVVDFEALSKGYGDRLLIDDLTFKLPPGGIVGVIGPNGA
                     GKTTLFRMITDQEKPDKGTIKIGDSVRLGYVDQSRDSLDGKKTVWEEISDGNDMILLG
                     KKEMNSRAYCASFNFKGADQQKKVGSLSGGERNRVHLAKMLRSGANLLLLDEPTNDLD
                     VDTLRALEEALEDFAGCAVIISHDRWFLDRIATHILAFEDDSHVEWFEGNFQDYEQDK
                     MRRLGTDSVIPHRLKYKKFSR"
     gene            811..1395
                     /locus_tag="VFL49_09575"
     CDS             811..1395
                     /locus_tag="VFL49_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HET7912269.1"
                     /translation="MSMAADDAVPPRQIRGLRPLPQIIFSSRWLQLPLYLGLIVAQGV
                     YVILFMKELWHLVHGAMNLTEQEIMLVVLALIDVVMISNLLIMVIVGGYETFVSRLEL
                     ERHPDQPEWISHVNASVLKIKLAMAIIGISSIHLLRVFIYAGSLGKPDAPYTETGVMW
                     QALIHGLFVLSAIGIAIVENVSQTAYARARPHQH"
     gene            1453..3789
                     /locus_tag="VFL49_09580"
     CDS             1453..3789
                     /locus_tag="VFL49_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET7912270.1"
                     /translation="MNKPIRAPKKPFVLEEATIDELHAAIKAGETTCVDVVRHYLARV
                     RAFNGTSNLLVTKDGAPVPEASGTVRGGAPLKFPTQTVKASELLPDLDRYKGPPLEFG
                     RMEATASDPSVQQQFGLTVGQPNAGQVNALATLNIRGERSVTCRGDFDLHPSKGPLPK
                     GAPPVCEYFRHLPDALERAAELDAAYGRNPDLEKMPMYGVVFSFKDPFDTKDMRSTGG
                     GDARYDIDFPARDHVLVEQLRNKGAIIFAKAVNTEYNGRPDDPGGSHKPDKVLTSTLG
                     YQRSTWAGNPSNPYDTTRAASLGSSSGSGVSVSTNMVMCSLGEETRASCRGPANHNAV
                     ALILPHKALLGFDGGAIGADIHVDRSGILARTIGDAAKVLDALKDPAQGYYDPRDPFT
                     TVPRSAVLDSYLRHAKPAASLKGLRIGVIRESMIVRPGNLTEMPVTTAATQEIKSVLA
                     GKLGATLIESSDPLWQRDPDIEQMTVDYRVALARLVPVFMPDILFRLTADGQPVFKEF
                     AAAIRPTEFEPGKVFGSGKMKPIDYILDMAEGRIPPPSNLDLATVQHQAPQTAFRFHI
                     NQYLSRRAADWKAKGFTETLTDFTELNERSKFWGDDQRASWLNWDEVTDLRNPLGERQ
                     NITERIMLRELLRRADMMVTLENRLDVLVRLHTPWPPGKIGGAGQGFRNNLTLESLSG
                     PNAGLTEVLIPAGFVTTVYDPVYQLSADGKSYLHVPSNTPATIPEPGLPFSLVFRAEP
                     GKEDVLLHVAASYEAASRRRIPPPAFGPLPVEQRARSD"
BASE COUNT          758 a         1203 c         1127 g          727 t
ORIGIN      
        1 gagcgtctcg gcgccaacgt ggtcgatttc gaagctctca gcaaaggcta tggcgatcgc
       61 ctgctgatcg acgatctcac cttcaagctg ccgccaggcg gcattgtcgg cgtgatcggc
      121 ccgaacggcg ccggcaagac cacgttgttc cgcatgatca cggaccagga aaagcccgac
      181 aaaggcacga tcaagatcgg cgactccgtg cgtctcggtt atgtcgacca gtcgcgcgac
      241 agcctcgacg gcaagaaaac cgtttgggag gaaatctccg acggcaacga catgatcctc
      301 ctcggcaaga aggaaatgaa ctcacgcgcc tattgcgctt cgttcaattt caaaggcgcc
      361 gatcagcaga aaaaagtcgg ttcactgtca ggcggcgagc gcaaccgcgt gcatctcgcc
      421 aagatgctgc ggtcgggagc gaaccttctg ctgctcgacg agccgaccaa cgatctcgac
      481 gttgatacgc tacgcgcgct ggaagaagcg ctggaagatt tcgccggctg tgcggtgatc
      541 atcagccacg accgctggtt tctcgaccgg atcgcgacac atattctggc ctttgaggac
      601 gacagtcacg tcgagtggtt cgaaggaaat ttccaggact acgaacagga caagatgcgg
      661 cgtttaggca cagactcggt gattccgcac cgattgaagt acaaaaagtt cagccgctga
      721 gcggccgcca tccttcggcc attccgaatc gcataagttc cgccggcgtc cgttctggac
      781 gcatgacgca ttcaaatggg gtgaccaacc atgtcgatgg ctgccgacga cgctgttcct
      841 ccgagacaaa ttcggggcct gcgtccgctt ccccagatca tcttcagctc gcgctggctg
      901 caactgccgc tttacctcgg cctcatcgtg gcgcagggcg tctatgtcat cctgttcatg
      961 aaagagctct ggcatcttgt tcatggcgcc atgaacctca ccgagcagga gatcatgctc
     1021 gtggtgctgg cgctgatcga tgtggtgatg atctccaatc tcctgatcat ggtgatcgtc
     1081 ggcggctacg agacgttcgt gtcgcgcctc gaactcgagc ggcatcctga ccagcccgaa
     1141 tggatctcac acgtcaatgc cagcgtgctg aagatcaagc ttgccatggc gatcattggc
     1201 atctcctcga tccacctgct gcgcgtgttc atctatgcgg gctcgcttgg aaagcccgac
     1261 gcgccctaca ccgaaaccgg tgtgatgtgg caggcgctga tccatggtct cttcgtgtta
     1321 tccgcgatcg ggatcgccat cgtcgagaac gtcagtcaaa ccgcctatgc acgggcgcgg
     1381 ccgcaccagc actaaacgcg cttccactcg ctggtctaaa tctctaacct tggcgggaca
     1441 gggaacaccg ccatgaacaa gccgatccgc gcgccgaaaa agcccttcgt cctcgaagag
     1501 gcgacgatcg acgaacttca tgccgcaata aaggcgggcg aaaccacctg cgtcgatgtc
     1561 gtacggcatt accttgcgcg cgtgcgcgcc tttaacggca catccaatct gctggtcacg
     1621 aaggatggcg cacccgttcc cgaagcgagc ggtaccgtgc gcggcggtgc accgctgaaa
     1681 tttcccacgc aaaccgtcaa ggcgtcggaa ctgctgcccg atctcgacag gtacaagggg
     1741 ccgccgcttg agttcggccg catggaggcg accgcgtcgg acccttcggt gcagcagcaa
     1801 ttcggactga cggtcggcca gccgaacgcc ggtcaggtca atgcgcttgc cacgcttaac
     1861 attcgcggtg agcgctcggt gacgtgtcgc ggcgatttcg acctgcatcc ctcgaaggga
     1921 cccttgccga aaggtgcgcc gccggtgtgc gaatatttcc ggcatttgcc tgatgcgctc
     1981 gagcgcgcgg cggaactcga cgccgcttac ggcagaaatc cggaccttga gaagatgccg
     2041 atgtatggcg tcgttttctc gttcaaggat ccgttcgaca ccaaagacat gcgctcgacc
     2101 ggtggcggcg acgcccgtta cgacatcgat tttccggccc gcgatcacgt tctcgtggag
     2161 cagctgcgca acaagggcgc gatcatcttc gccaaggcgg tgaacaccga atacaacggc
     2221 cggccggatg atccgggcgg atcgcacaag ccggacaaag ttctgacatc gacgctcggt
     2281 tatcagcgca gcacctgggc cgggaatccg tccaatccat acgatacgac ccgcgcggcg
     2341 tcccttggtt caagctccgg ctcgggcgtt tcggtgtcga ccaacatggt gatgtgcagc
     2401 ctgggcgagg aaacgcgcgc gtcctgccgc gggcccgcca atcacaatgc ggttgcgctc
     2461 atcctgccgc acaaagcatt gctcgggttc gatggcggcg ccattggcgc cgacatccat
     2521 gttgaccgct ccggtattct cgcgcgcacg atcggcgatg cggccaaggt gctcgatgcg
     2581 ctcaaggatc ctgcgcaagg ctattacgat ccgcgtgacc ccttcacgac ggtgccgcgc
     2641 tcggcggtac tcgacagcta tctacgtcat gccaagccgg ccgcctcgct gaaaggcctg
     2701 cggatcggcg tcatccgcga atcgatgatc gtgcgtcccg gcaatttgac ggaaatgccg
     2761 gtcacgactg cggccacgca agaaatcaag tccgtcctcg ccggcaagct tggcgccaca
     2821 ctcatcgaat cgtccgatcc gctctggcag cgcgaccccg atatcgagca gatgacagtg
     2881 gattatcgtg tcgcccttgc gcggctggtg ccggtcttca tgcccgatat tcttttccgg
     2941 ctgaccgccg acggacaacc tgtattcaag gaattcgcgg ccgcgatccg gccaaccgag
     3001 ttcgagccgg gaaaagtctt cggttcggga aagatgaagc cgatcgacta catactcgac
     3061 atggccgagg gccgcattcc gccgccatcg aatctcgatc ttgccacggt ccagcatcag
     3121 gcgccgcaga ccgcgtttcg cttccacatc aatcagtatc tgtcgcgccg cgccgccgac
     3181 tggaaagcaa aagggtttac ggaaacgctc accgatttca ccgaactcaa cgagcgatcg
     3241 aaattctggg gcgatgatca gcgcgcgagc tggctgaact gggacgaagt caccgatctg
     3301 cgcaatccgc tcggcgagcg gcagaacatc accgagcgca tcatgctgcg cgagcttttg
     3361 cggcgcgccg acatgatggt gacgctggaa aaccggctcg atgtgctggt gcggctgcac
     3421 acgccctggc cgcccggcaa aatcggcggg gccgggcagg ggtttcgcaa caacctcacg
     3481 ctggaatcgc tgagtgggcc gaatgccggg cttaccgagg tgctcatccc ggcgggcttt
     3541 gtgacgacgg tctacgatcc ggtttatcag ctcagtgccg acggcaagtc ttaccttcac
     3601 gtgccgtcaa acacgccggc cacgattccc gagcccggcc tgccattttc gctggtgttc
     3661 cgcgccgagc ccggcaagga agacgtcctc cttcatgtcg cagcatcgta tgaagccgca
     3721 tcgcggcggc gtattccgcc gcccgcattc gggccgctcc cggtggagca gcgcgcgcgt
     3781 tcggactagt ttgtcggcat gactggacgc accgt
//
LOCUS       DASSRL010000471         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_204799, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000471 DASSRL010000000
VERSION     DASSRL010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204799"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1177)
                     /gene="waaF"
                     /locus_tag="VFL49_09585"
     CDS             complement(116..1177)
                     /gene="waaF"
                     /locus_tag="VFL49_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET7912271.1"
                     /translation="MSQELNQAAPVVIIPFAWIGDFVRCHSVVRLLRMQNPDRSVDIV
                     SSTLCAPLADYMPGIRRAIVADLPRRRLGIATQHQLADQLREGQYAQALVMSRKWKAA
                     LAPWLAGIPLRTGFAGEFRFGLLNDVRFGERKLPRMIDQMGALALPKDAPSPAEWPLP
                     ELKVPAHEVQTWLARQRLAGENRPIVTLSPGAVGAGKAWPVVHYTELAKSLATGGASV
                     WILGGPAETPLAKKIATAAGDRVRDLTGHDLRNAILALAAADISVTNDSGLMHVSAAI
                     GIPTIAIFGPTSPWHWKPLNPVAAILEPPDDDEARQRARIEGNDAVRHRRTDGVAVGE
                     VLAAVKRILAEGKNGVGPG"
     gene            1380..>2066
                     /gene="rfaD"
                     /locus_tag="VFL49_09590"
     CDS             1380..>2066
                     /gene="rfaD"
                     /locus_tag="VFL49_09590"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HET7912272.1"
                     /translation="MFLVTGGAGFIGSNIVASLNEAGSSDVVVSDLLGRDDVKWRNLA
                     RHQVADLVPPAELAQWLERRKLDAVIHMGAISDTTATDGDLVMENNFRLSLRLLDWCT
                     QTRTPFIYASSAATYGDGSHGFDDDWSLEALRSLKPMNLYGWSKHLFDLAVVDRYMKK
                     EKMPPHWVGLKFFNVYGPNEYHKGHMASVLAKVFDLAKSGQPVKLFKSHRDGIADGDQ
                     RRDFIYVDDAV"
BASE COUNT          380 a          637 c          641 g          408 t
ORIGIN      
        1 gccgacgcga tcgaatcctg cgcgcggtca tctgtcatcg aggcggactt cagggttgcc
       61 ataacgtcct gatagcacgg aaagaatgtc gcgtcaggtt tgcgaacgcc attcgttaac
      121 ccggacccac gccgtttttg ccttctgcga gaatccgctt tacggccgca agcacctcgc
      181 cgacggcgac gccgtcggtg cgcctgtggc ggaccgcgtc gttaccctcg atacgcgcgc
      241 gctgccgcgc ttcgtcgtca tcgggcggtt ccaggattgc cgcgaccgga ttgagcggct
      301 tccagtgcca tgggctggtc ggtccgaaaa ttgcgatcgt cggaattccg atggccgccg
      361 atacgtgcat caggccggaa tcgttggtca ccgaaatatc ggcggcggcc aacgccagaa
      421 ttgcgttgcg caggtcgtgc ccggtgaggt cacgcacacg atccccggcc gccgtcgcaa
      481 tctttttcgc caagggcgtt tccgctggtc ctccgagaat ccagaccgac gcgccgcccg
      541 tcgcgagtga ctttgccagt tcggtgtagt ggacgactgg ccatgctttt cccgcaccaa
      601 ccgcgccggg ggacagcgtt acgatcggcc ggttttcccc tgcgagccgc tgccgcgcga
      661 gccaggtctg gacttcgtga gccggcactt tcaattcggg caacggccac tcggccggcg
      721 acggggcgtc tttcggcagc gcaagcgcgc ccatctggtc gatcatgcgt ggtagcttgc
      781 gctcgccaaa acgaacatcg ttgagcaggc cgaagcgaaa ctcgccggca aatccggtcc
      841 gtagcggaat tccggccagc catggcgcga gcgccgcctt ccacttgcgc gacatgacca
      901 gtgcctgtgc gtattggcct tcgcgtaatt gatccgcaag ctgatgctgt gtggcgatac
      961 caagacggcg ccgcgggaga tcagcgacga tcgccctgcg aattcccggc atgtagtcgg
     1021 cgagcggcgc gcatagtgtg ctgctgacga tatcgacaga ccgatcggga ttctgcatgc
     1081 gcagcagcct gaccactgaa tgacagcgga cgaaatcgcc gatccatgca aaggggatga
     1141 tcaccaccgg agcggcttga ttcagctctt ggctcatgga tctcgcgccg ccacgtcccc
     1201 caacgtcgcc ccgctggcgg ggccgacagg ggaggcgtta ccccgcgtcg tatccgcatg
     1261 tccagccgcg atttttaccc tgttgttggc gttttgcagg cccaaacggc ataagattgc
     1321 gcccggccgc cgactggggc aaaggtttgc tgcgaagctc aggtgccggc gaggggtaaa
     1381 tgtttttggt caccggcgga gccgggttta tcggctcaaa catcgtcgcc agcctcaatg
     1441 aggccggctc gagcgatgtc gtggtcagcg acctgctcgg ccgcgacgac gtcaagtggc
     1501 gcaatctggc gcggcatcag gtcgccgatc tggtgccgcc tgcggagctc gcgcaatggc
     1561 ttgagaggcg taagctcgac gcggtcatcc atatgggcgc gatctccgac accaccgcaa
     1621 cagacggcga tctggtgatg gaaaacaatt tccgcctttc gctgaggctc ctcgactggt
     1681 gcacgcagac gcgcacgccc ttcatctacg cgtcatcggc cgcgacctac ggcgacggca
     1741 gtcatggttt tgatgacgac tggtcgctcg aggccttgcg gagtctcaag ccgatgaatc
     1801 tttacggctg gagcaaacac ctgttcgacc ttgccgttgt cgatcgctac atgaaaaaag
     1861 aaaaaatgcc gccgcattgg gtcgggctga aatttttcaa tgtctatggc ccgaacgagt
     1921 atcacaaggg gcacatggcg agcgtgcttg ccaaagtctt tgatctggca aaatcgggtc
     1981 agccggtaaa gctgttcaag tcccaccgcg atggtatcgc cgatggtgac cagcgccgcg
     2041 acttcattta tgtagacgac gcggtt
//
LOCUS       DASSRL010000472         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1377140, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000472 DASSRL010000000
VERSION     DASSRL010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377140"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="lpxA"
                     /locus_tag="VFL49_09595"
     CDS             complement(<1..653)
                     /gene="lpxA"
                     /locus_tag="VFL49_09595"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET7912273.1"
                     /translation="MSAHMIDPSARIEAGATIGKDVLIGPYCTIGAQVSVGDGCRLIG
                     HVNLTGHTTIGPRTVIYPFASLGSPPQSYSYRGGPTRLVVGAECDIRENVTMSIGTED
                     GGGVTEVGDRCFFMVGSHVGHDCKVGNHVVFANNTVLGGHVTIGDHVVFGGGVAVRQF
                     VRIGEGAMIVGLSGVRADVIPFGLAHGPLADLIGLNVIGMRRRGVSKADVQRVRSAY"
     gene            complement(650..1105)
                     /gene="fabZ"
                     /locus_tag="VFL49_09600"
     CDS             complement(650..1105)
                     /gene="fabZ"
                     /locus_tag="VFL49_09600"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET7912274.1"
                     /translation="MNKPVTRLEAADIAMVLKLLPHRYPFLMVDRVIDIRGDEHGIGI
                     KNVTINEPQFQGHFPGNPVFPGVLMIEGMAQTAGVLCIAATGAHPASVFFLTIDKAKF
                     RKPVRPGDTIEYHMDRITRRKSMWWYRGEAKVAGQVVAEAQVGAMLSDA"
     gene            complement(1116..2171)
                     /gene="lpxD"
                     /locus_tag="VFL49_09605"
     CDS             complement(1116..2171)
                     /gene="lpxD"
                     /locus_tag="VFL49_09605"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET7912275.1"
                     /translation="MSEPIFLRQSAGLTVDEIAGLTGAAIASRSPRATRIMNIAPLDR
                     AGPRDLTFFEGRNFAAKAAATHAGACLTTAELAKELPGRVSVLTVREPYRAFVTVARQ
                     LFPHALRPSSLSKPDDFAGAHVDVSARAEDDVNIEPGAVIGPQAEIGSGTSIGANAVI
                     GAGVRIGRDCSIGANTTISNALIGDRVIIHPGCHIGQDGFGFVMDGKGHLKVPQIGRV
                     IIQDDVEIGAGTTVDRGAIRDTVIGEGTKIDNLVQVGHNVSIGRHCVIVAQTGISGSS
                     TLEDFVVLGARVGLNTNVTIGEGAQIAGVSVVHGDVPAGARWGGTPAKPVKQWFREMM
                     ILERLARQKGGADAGDK"
     gene            complement(2230..>4517)
                     /gene="bamA"
                     /locus_tag="VFL49_09610"
     CDS             complement(2230..>4517)
                     /gene="bamA"
                     /locus_tag="VFL49_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET7912276.1"
                     /translation="GLKALVATGLFSDVRISHAGGRLVVTVAENPVINRVAFEGNKKA
                     KDEQLQAETQSKPRGTLSRPTVQADVQRIIEIYHRSGRFDVSVDPKIIELPNNRVDLV
                     FEIKEGDKTGIKDIRFVGAKAFSHGRLKDVIKTSESNLLSFLQTTDIYDADRVEADRD
                     LLRRFYLKHGYADVRIVSAIGEYDPAKKGFVVTFTIDEGNQYRVGTVDVVSNVRAIDP
                     AALRGQVKLSPGNVYNADLVEKSVESMTIQAARQGYAFANVRPRGDRNFEAKTINLVF
                     VVEEGARAYIERINIRGNTRTRDYVIRREFDLGEGDAYNRALIDRAERRLKNLNYFKS
                     VKITNEPGSAPDRVVINVNVEEQPTGEFSISGGYSTADGLIGEISVADRNLMGRGQYA
                     KASVTYGERVRGFDLSFVEPFLFGYRMAGGIDLFARQNLASNYVSYDSQTIGTNLRLG
                     FALTEEIAFQPRYTFYQQKITLPTQYNNCQFSNFAPATGLAPGTGVNPGVEFGGLDTQ
                     VNVPFGCYADGEASLAVRKELAAGPVNVSMLGYTVSYNTLDNNRVPTSGLFAELKQDF
                     AGIGGDVNFIRTSAEARTYYEVFNDVVSVFKVQGGHIAGWGGNDLRMLDHFFMGPNLV
                     RGFSPAGIGPRDLTPGTTNDALGGTLYWGASVEAQTPLYFLPKDIGIKVAVFADAGSL
                     WNYQGPTSWSVTGETLQVGLNSPSMIRSSVGVGLLWDSPLGPLRFDLAYPITKYCATP
                     VGGGEVCDRTQVFRFSGGTKF"
BASE COUNT          905 a         1438 c         1302 g          872 t
ORIGIN      
        1 tgatacgccg agcgcacgcg ctgaacgtcc gcttttgaaa ctccccgccg ccgcatgccg
       61 atcacattca ggccgatcag gtcggccaat ggcccgtgtg ccaagccaaa tggaatgaca
      121 tcggcacgca cgccgctcag accgacgatc atggcgccct cgccgatgcg aacgaactga
      181 cggaccgcaa cgccgccgcc aaaaacaacg tgatccccga tcgtcacatg accgccgaga
      241 acggtattgt tggcaaagac gacgtgattt cccaccttgc aatcatgccc gacatgcgac
      301 ccgaccatga agaagcatcg atctccgact tcggtgacgc caccaccatc ctctgtgccg
      361 atactcatgg tcacattttc acggatgtca cattcggcac ccaccaccag ccgagtaggc
      421 ccgccgcgat agctgtacga ctgcggcgga gacccgagcg aggcaaacgg gtaaatcacc
      481 gtccgcggtc cgatcgtggt gtggccggtc agattgacat ggccaatcag gcggcacccg
      541 tcgccgacgc tcacctgcgc gccgatcgtg cagtacggac cgatgagaac atcttttccg
      601 atcgtggccc cggcttcgat ccgcgccgag gggtcgatca tgtgcgcgct catgcgtcgg
      661 acagcatggc cccgacctgg gcttcggcga caacctggcc ggccactttc gcttcgcccc
      721 gataccacca catggactta cgtcgtgtaa tgcggtccat gtgatattcg atggtgtcgc
      781 ccggccgcac aggcttgcgg aatttcgcct tatcaatggt gaggaaaaac accgatgccg
      841 ggtgcgcgcc cgtcgccgcg atgcagagca cacctgccgt ctgcgccatg ccctcgatca
      901 taagcacgcc ggggaacaca ggattgcccg gaaagtgacc ttgaaattgc ggctcgttga
      961 tcgtcacgtt cttgataccg atgccgtgct cgtcgccacg gatgtcgatc acccggtcaa
     1021 ccatgagaaa cggataacgg tgcggaagca gcttgagcac catcgcaatg tccgccgcct
     1081 cgagccgggt cacgggtttg ttcatcacaa acgtctcatt tgtcgcccgc gtccgcgccg
     1141 cccttctgcc gcgcaagccg ctcaaggatc atcatttcac ggaaccattg cttcacgggc
     1201 tttgccggcg ttccgcccca tcgcgcgccg gccggcacat caccgtggac cacgctgacc
     1261 cccgcgatct gcgcgccctc accgatcgtg acattggtat tcagaccaac ccgcgcgccc
     1321 aacaccacaa aatcctcgag agtcgaactg cctgaaattc cggtctgcgc aacgatgacg
     1381 caatgtctgc caatgctcac attgtggccc acctgcacca ggttgtcgat cttcgttcct
     1441 tcgccgatga cggtgtcgcg aatggctccg cgatccacag tcgtcccggc gccgatctcg
     1501 acatcatcct ggataatcac gcggccaatc tgaggcacct tgagatgccc cttgccgtcc
     1561 atcacaaagc cgaagccatc ctgcccgata tggcagcctg gatgaataat gacgcgatcg
     1621 ccgataagag cgttgctgat cgtcgtattg gcaccgatcg aacaatcgcg gccgatccgt
     1681 acgcccgcgc cgatcaccgc atttgcgccg atggatgtcc cgctgccgat ttcggcctgc
     1741 ggaccaatca cggcacccgg ctcgatgtta acgtcatctt ccgcgcgggc actgacatcg
     1801 acatgagctc cggcaaaatc gtcgggcttg gataaagaag acggccgtaa agcgtgcgga
     1861 aacagctggc gcgcgaccgt gacgaacgca cggtagggct cgcggacggt gagcaccgaa
     1921 acgcggcccg gcaattcctt ggcaagctca gccgtcgtca ggcacgcgcc ggcatgtgtc
     1981 gcagctgcct tcgccgcaaa attccggccc tcgaaaaatg tgagatcgcg cggcccggcc
     2041 cggtcaagcg gcgcaatatt catgatgcgc gtcgcccgcg gcgagcgcga agcaatcgcg
     2101 gcgccggtca ggcctgcaat ctcatcaacc gtgagacctg cggactgtcg taggaatatg
     2161 ggctcgctca tcctgccgga atccgacccg tccatgcctg gcgtcgccaa gttcggaact
     2221 tgttcttgct cagaacttgg tgccgccact gaagcggaaa acctgcgtgc gatcgcacac
     2281 ttcaccgcca ccgacgggcg tcgcgcagta tttcgtgatt gggtaggcca ggtcaaaacg
     2341 caatgggccg agcggcgaat cccacaggag accgacgccg accgaactac ggatcatcga
     2401 cgggctgttg aggccgacct gaagcgtttc gccggtcacg ctccagctcg tcggcccctg
     2461 ataattccac aatgagcctg cgtccgcgaa aaccgcgacc ttgatcccga tgtctttcgg
     2521 caggaagtac aacggcgtct gcgcctcaac gcttgcgccc caatagagcg tgccgccgag
     2581 cgcgtcattg gtcgttccgg gcgtaaggtc gcgcggacca atgccggccg gtgaaaagcc
     2641 gcgcacgaga ttggggccca taaagaagtg gtccagcatg cgcaggtcgt tgccgcccca
     2701 gccggcaata tggccgccct gcaccttgaa gacgctgacc acgtcgttga acacttcgta
     2761 gtaggtgcgc gcttcggccg acgttctgat gaaatttacg tcaccgccga tgccggcaaa
     2821 gtcctgcttg agctcggcga agaggccact ggtcgggacc ctgttgttgt cgagcgtgtt
     2881 gtaggaaacc gtgtagccaa gcatcgaaac gttgacggga cccgcggcca attccttgcg
     2941 gaccgcaagc gaggcctcac cgtcagcata acagccgaat ggaacgttta cttgcgtatc
     3001 caaaccgccg aattcaacgc ccggattcac accggtgccg ggcgcaagac cggttgccgg
     3061 agcgaagttc gaaaattgac agttgttgta ctgggtcggc agcgtgatct tctgctggta
     3121 gaatgtgtag cgcggctgga acgcgatctc ttccgtcagc gcaaagccaa gccgcaggtt
     3181 ggtgccgatc gtttggctgt catacgaaac gtaatttgac gccaggttct gccgcgcaaa
     3241 aaggtcgatg ccgcccgcca tgcgatagcc gaacaggaac ggctcgacga aggacaggtc
     3301 gaagccgcgt acgcgctcgc cgtaagtgac cgatgccttt gcgtattggc cgcgacccat
     3361 caggttgcgg tcggccacgc tgatttcgcc gatcagaccg tcagcggtcg agtacccgcc
     3421 ggagatggag aactcgccgg ttggctgttc ttcaacattg acattgatca cgacacgatc
     3481 cggcgccgat cccggctcgt tggtgatctt gacgctcttg aaatagttca ggttctttaa
     3541 tcggcgctcg gcgcgatcaa tcaatgcacg gttataggca tcgccctcgc cgagatcgaa
     3601 ttcgcgcctg atcacatagt cgcgggtgcg tgtattgccg cggatgttga tgcgctcgat
     3661 gtaagcgcgg gcgccctctt cgacgacgaa gaccagattg atcgtcttcg cctcgaagtt
     3721 acggtcgccg cggggacgca cgttggcaaa cgcatagccc tgccgcgccg cctgaatcgt
     3781 catcgactcg acgctctttt cgacaagatc ggcgttgtag acgttgccgg gactgagttt
     3841 gacctggccg cgcaaagccg cgggatcgat agcgcggaca ttggacacga catccacggt
     3901 cccgacccgg tattggttgc cctcgtcaat cgtaaaagtg accacgaacc ccttcttggc
     3961 gggatcgtac tcgccgatcg cagacacgat ccgcacatcg gcatagccgt gcttcaggta
     4021 aaacctgcgc agcaggtcgc ggtcagcctc gacacggtcg gcatcgtaaa tatcggttgt
     4081 ctgcaggaag ctcagcaggt tgctctcgga cgtcttgatg acgtctttca gccgcccatg
     4141 cgaaaacgcc ttggcgccga caaaccggat atccttgatg ccggttttgt cgccttcctt
     4201 gatttcgaac accaggtcga cgcggttgtt cggcaattcg atgatctttg gatcaaccga
     4261 gacgtcgaag cggccgctgc ggtgatagat ttcgataatc cgctggacgt cggcctgcac
     4321 ggtcgggcgc gaaagagtgc cgcgcggctt cgactgggtt tctgcctgga gttgttcgtc
     4381 cttcgctttt ttgttgccct cgaacgcgac ccggttgatc accgggttct cggccacggt
     4441 caccaccagg cggccgccgg catggctaat ccggacatcg gaaaaaaggc cggtcgcgac
     4501 cagcgccttg agccctt
//
LOCUS       DASSRL010000473         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_467699, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000473 DASSRL010000000
VERSION     DASSRL010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467699"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFL49_09615"
     CDS             complement(<1..404)
                     /locus_tag="VFL49_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt efflux protein"
                     /protein_id="HET7912277.1"
                     /translation="MPDSDPPGSRPAGAPSYEPRNLPVPVPPARNESWFTRALRALFG
                     WKPNIVRADLQDILDAIGPGESGFSPEESRMLRNILGLRERRVGDVMVPRADIIAVQQ
                     DIKLGELMRVFEGAGHSRLVVYNDTLDDPIGMV"
     gene            complement(449..961)
                     /gene="ybeY"
                     /locus_tag="VFL49_09620"
     CDS             complement(449..961)
                     /gene="ybeY"
                     /locus_tag="VFL49_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7912278.1"
                     /translation="MMKTPPGRRTSPPHIDIRIQSPLWDAQPLAEKTVRNSILAAAAA
                     LSTAGGEVSIVLADDSAVRSLNRDWRGIDKPTNVLSFPGAGPATGGDTRLLGDIVIAF
                     ETLERECTDESRDFLHHLAHLAVHGFLHLHGFDHQSDAQAEEMERLESRIMARLNMPD
                     PYRAHDLGNA"
     gene            complement(958..1965)
                     /locus_tag="VFL49_09625"
     CDS             complement(958..1965)
                     /locus_tag="VFL49_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7912279.1"
                     /translation="MIPPAAASEETATTQTVLAFEDNKLASVLFGQYGQNLALIERRL
                     GVVAEQRGNQVTIEGSRGACEQARHVLEGLYEQLKRGRDLSQGDVEGAIRQAIAQGSL
                     FDYDPAAPRPAFEEINLRKRPVRARTAAQDAYIRALKRHALVFGTGPAGTGKTWLAVA
                     QAIQMFERKEVDRIILSRPAVEAGERLGFLPGDMREKVDPYLRPIYDALYDLMDARIV
                     ERALQAGEIEIAPLAFMRGRTLSNAAVILDEAQNTTSMQMKMFLTRLGENSRMIVTGD
                     PSQVDLPHGQASGLAEAVKLLNGIEGVGHVAFTHEDVIRHELVARIVEAYDRGPVRGG
                     R"
     gene            complement(1991..3376)
                     /gene="miaB"
                     /locus_tag="VFL49_09630"
     CDS             complement(1991..3376)
                     /gene="miaB"
                     /locus_tag="VFL49_09630"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET7912280.1"
                     /translation="MSKARKVHVKSFGCQMNVYDSHRMADTLAPAGYTETASPEDADL
                     VILNTCHIREKAAEKVYSEIGRIRELKEAAARDSRRMLIAVAGCVAQAEGREILRRAG
                     AVDMVVGSQNYHRLPEMVARAQRGEKVIDTEFPVEDKFDALAAPKPDAIARRGISSFV
                     TVQEGCDKFCTFCVVPYTRGAEVSRPVEKIAAEVERLARGGVREVTLIGQNVNAYHGL
                     GPDGAASSLGKLLRRLDQIPGILRLRYTTSHPSDMDDDLIAAHRDLPALMPYVHLPVQ
                     SGSDRVLAAMNRKHTRKDYLEVVARLRAGRTDLAFTSDFIVGFPGETESDFRDTLTLV
                     DDIGYAGAFSFMYSPRPGTPAAEMEEQIPAEVKYERLQRLQAAITRHQRRFNAGFAGR
                     TVDVLLEKPGKLPGQLVGRSPYLQAVQVMAPAECIGSMRRVTIDEVGSNSLFGSLADE
                     NRAMRLTAAGA"
     gene            complement(3497..4261)
                     /locus_tag="VFL49_09635"
     CDS             complement(3497..4261)
                     /locus_tag="VFL49_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7912281.1"
                     /translation="MTPLLIFVQWLLSKLRLPGWGFIASNYYRVLCSLLRISVRVSGV
                     PTHDRAVLYVSNHVSWADILVIGSLAPVAFVAKREVANWPLVGITAKLQRTVFVDRAR
                     RHQTGAAVAEIVKRLADGVSVVLFAEGTSSDGNRVLPFRSALLGAVEEASARTGNILI
                     QPMSISYTGLHGIPMGRQHRPLVAWYGDLDFLPHIKAFIEHGAVDAVVSFGDTVPADA
                     AVDRKAMTKRLERSVRGLLASALRGRALTVHNAHPG"
BASE COUNT          793 a         1330 c         1392 g          814 t
ORIGIN      
        1 accatgccga tgggatcgtc gagtgtatcg ttgtacacca caagccgcga atggcctgcg
       61 ccctcgaaca cgcgcatcag ttcgccaagc ttgatgtcct gctggacggc gatgatgtcg
      121 gcacgcggca ccatgacgtc gccgacacgg cgttcacgca ggccaaggat gttcctgagc
      181 atccggcttt cttcggggct gaagccggac tcgccgggcc cgatggcatc aaggatgtcc
      241 tgcagatcgg cgcgcacgat gttcggcttc catccgaaca gtgcgcgcag ggcgcgggta
      301 aaccagctct cgttgcgcgc gggcggcacc ggtaccggca ggtttcgcgg ctcgtatgac
      361 ggcgcgccgg cgggcctcga accaggtggg tcgctgtcag gcatggctat cgggcatggc
      421 tatcggacat ggctatcgga catggcggtc aggcgttgcc aagatcgtga gcgcggtagg
      481 gatcgggcat attcaagcgg gccatgatcc tgctttcgag tctttccatt tcctcggctt
      541 gcgcatcgga ctgatgatca aagccgtgga ggtgaagaaa cccatgcact gcgagatggg
      601 caaggtgatg aagaaaatcg cggctctcat cggtgcattc ccgttcgagt gtctcaaagg
      661 cgatcacgat atcgcccagc aatcgcgtgt ctccgccggt ggcgggaccc gcgccgggaa
      721 acgacaggac gttggtcggc ttgtcgatgc cgcgccagtc ccggttgagt gagcggacag
      781 ccgaatcgtc ggcgagcaca atactgactt cgccgcccgc tgttgagagt gccgcggccg
      841 cagcaaggat ggagttccgg acggtctttt cggccagggg ctgggcgtcc caaagcgggg
      901 actgaatccg gatatcgatg tgtggcggcg acgtgcgccg ccccggcggc gtcttcatca
      961 tcgtcccccg cgcacggggc cgcgatcgta agcctcgacg atgcgggcca cgagctcgtg
     1021 gcggatcaca tcctcatgcg tgaacgccac atgaccaacg ccttcgatgc cgttcagcaa
     1081 tttgacggct tccgccaggc ccgacgcctg gccgtgcggc agatcgacct ggcttggatc
     1141 gcccgtgacg atcatacggc tgttttcacc gaggcgtgtg agaaacattt tcatctgcat
     1201 ggaggtcgtg ttctgcgcct catccagaat aacggcggcg ttcgaaagcg tgcgtccacg
     1261 catgaaggcg agcggcgcga tctcgatttc gccggcctgc agcgcccgtt cgacgatgcg
     1321 cgcgtccatc aaatcgtaaa gcgcgtcgta gatcggacgc agatacgggt cgaccttttc
     1381 gcgcatgtcg ccgggaagaa agccgagacg ctcgccggcc tcgacggcgg ggcgcgacaa
     1441 tatgatgcgg tcgacctctt tgcgctcgaa catctgaatt gcctgcgcga ccgcgagcca
     1501 ggtcttgccg gtgccggccg gcccggttcc gaaaaccaga gcgtggcgct tgagcgcgcg
     1561 gatataggca tcctgtgcgg ccgtgcgggc ccgcaccggc cgcttgcgca ggttgatttc
     1621 ctcgaacgcc ggccgcggtg cggcagggtc atagtcgaac agcgagccct gcgcgatcgc
     1681 ctggcgtatc gcgccttcga cgtcgccttg gctcaagtcg cggccgcgct tgagctgctc
     1741 atacagcccc tcgagaacat ggcgcgcctg ctcgcaggcg ccacgcgagc cttcaatcgt
     1801 gacctgattg ccccgctgtt cggcgacaac gccgagacga cgttcgatca gcgccagatt
     1861 ttgtccgtat tgtccgaaca gtaccgaggc gagcttgttg tcttcgaacg caagcacggt
     1921 ctgggtcgtg gccgtctcct cggaggcggc ggcgggcggg atcacgggat ccgttgtgcg
     1981 caagggtggc tcaggctcct gctgctgtaa gtctcattgc gcggttttcg tccgcgaggc
     2041 tgccgaagag gctgttgctg ccgacttcat cgatggtgac gcggcgcatt gagccgatac
     2101 attcggccgg tgccatgacc tgcacggcct gcaaatacgg tgaccggccc acgagttgtc
     2161 cgggcagttt gcccggcttt tcgagaagca catcgacggt gcggcccgca aagccggcgt
     2221 tgaatcggcg ctgatggcgt gtgattgcgg cctgcagccg ctgcaggcgt tcgtatttca
     2281 cctcggccgg aatctgctct tccatttcgg cagccggcgt gccggggcgg ggcgaataca
     2341 tgaaggagaa tgcgccggcg tagccgatgt catcgacaag cgtgagggtg tcacggaagt
     2401 ctgactcagt ttcacccggg aagccgacaa tgaaatcaga cgtgaaggcg aggtccgtgc
     2461 ggcccgcccg aagacgggct accacctcaa gatagtcttt gcgcgtatgc ttgcggttca
     2521 tcgccgccag aacgcggtcg gagccggatt gcaccggcag atgcacataa ggcatcagcg
     2581 ccggcaaatc gcgatgggcc gcgatcaggt cgtcatccat atcgctcgga tggctcgtcg
     2641 tatagcgcag acggaggatg ccgggaattt ggtcaaggcg gcgaagcagc ttgccaaggc
     2701 ttgatgccgc tccatccggc ccgagaccgt gataggcatt cacattctgt ccgatcagcg
     2761 tcacctcgcg tacgccgccg cgggccaggc gctcgacttc ggccgcgatc ttttcaaccg
     2821 ggcgggacac ttccgcgccg cgcgtatagg gcacgacgca gaatgtgcag aacttgtcgc
     2881 agccttcctg cacggtgacg aaggaggaga tgccgcgcct ggctatcgcg tcgggcttcg
     2941 gcgccgccag cgcatcgaac ttgtcctcga ccggaaactc ggtgtcgatg actttctcgc
     3001 cgcgttgcgc gcgcgcgacc atctccggca gccggtggta gttttgcgag ccgaccacca
     3061 tatcgacggc tcctgcgcgc cggaggattt cgcgaccctc tgcctgtgcg acgcagccgg
     3121 caaccgcgat cagcatgcga cggctgtcgc gtgctgcggc ttccttcaat tcccggatgc
     3181 gtccgatctc ggaatagact ttttccgcgg ccttctcgcg gatgtgacag gtgttgagga
     3241 tgacgaggtc ggcgtcttcg ggcgacgctg tctcggtgta gcccgccggc gccagcgtgt
     3301 ccgccatacg atgggaatcg tagacgttca tctggcagcc gaatgatttg acgtggacct
     3361 tgcgggcttt gctcatgcca tttcgatgcg aaacgtatcc aatttacctg ccgctccggg
     3421 atttgtcccg gaagaccata tatttgattt tacaacgatt ttaaggctcg gccggctcat
     3481 tcccgaaaat aaatcttcaa ccgggatgcg cgttgtgcac cgtcaaggcc cgtccgcgca
     3541 aggccgaggc caggaggccg cgcaccgacc tttccaggcg tttggtcatg gccttcctat
     3601 cgacggcagc atccgccgga accgtatcgc cgaaactcac gacggcatcg acggcgccgt
     3661 gctcgatgaa tgccttgatg tgcggaagaa agtcgagatc gccataccac gccaccagcg
     3721 ggcggtgctg gcggcccatc ggaatgccat gcagaccggt gtaactgatc gacatcggct
     3781 gaatcaggat gttgccggtg cgtgccgacg cctcttccac cgcgccgagc aaggcggagc
     3841 ggaacggcag aacgcgattg ccgtcgctcg atgtgccctc ggcaaacagt acaaccgaca
     3901 cgccgtccgc cagccgcttg acgatctcgg cgaccgccgc gccggtctga tgccggcgtg
     3961 cgcgatcgac gaacaccgtc cgttgcaatt tggcggtgat accgaccaga ggccagtttg
     4021 ccacctcgcg cttggcgacg aaggcgaccg gtgcgagcga gccgatgaca aggatatccg
     4081 cccacgagac atgattggaa acgtaaagga cggcgcgatc atgtgtcggc acgccgctga
     4141 cgcgcacgct gatgcggagc aacgagcaca gcacccgata gtaattcgaa gctatgaagc
     4201 cccaaccggg caacctgagc ttgctcagca gccactgcac gaagatcagc aggggggtca
     4261 tcgtgaagaa aaatgcagca atgaaaagcg cgcgcgccat cggcgttcag ttccggtgaa
     4321 tcatgccta
//
LOCUS       DASSRL010000474         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_450220, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000474 DASSRL010000000
VERSION     DASSRL010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450220"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFL49_09640"
     CDS             complement(<1..661)
                     /locus_tag="VFL49_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7912282.1"
                     /translation="MILGNGRSNGQFTPCEQCQLRKRPSLREFSTEELEFVKTFKVDE
                     LCVEPGISFLREGARSDHLYTVLRGWAFRYKVLDDGRRQILNYALPADMVGLQGTLMR
                     EMQHSVEALTPVVLCVFPRAKLWHLYSSLPSLAFDITWLAAREEQLIDEHLVSLGRRT
                     ALERTAFLLLHLFVRAEEAGLVKDASIQFPFTQQHVADTLGMSLVHTNKTLKRLFLTK
                     AI"
     gene            836..1210
                     /locus_tag="VFL49_09645"
     CDS             836..1210
                     /locus_tag="VFL49_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7912283.1"
                     /translation="MSGKRCLVLDDEFLIALDIQQILERAGAAHVASVASAAEAIELI
                     GRQSKFDVAVLDVKLGSTEHNGLDVAALLQTQGTPFVSLTGMRVDDAHAKRFPNAPVI
                     EKPYDAALLLSAVQRALVQGQN"
     gene            1348..3984
                     /gene="leuS"
                     /locus_tag="VFL49_09650"
     CDS             1348..3984
                     /gene="leuS"
                     /locus_tag="VFL49_09650"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7912284.1"
                     /translation="MTADSSKNERYNAREAEAHWQKIWAERGIFATKNDDPRPKYYVL
                     EMFPYPSGRIHMGHVRNYTMGDVVARFKRAKGNAVLHPMGWDAFGMPAENAAIDRKVH
                     PKAWTYDNIAAMKKQLQSMGLSLDWAREIATCDPAYYTHQQKMFLDFLKAGLVERKQS
                     KVNWDPIDQTVLANEQVIDGRGWRSGALVEQRELTQWFFKISTYSEDLLAALDTLDRW
                     PEKVRLMQKNWIGRSEGLLLRFMLDPATTPNKENELEIFTTRPDTLFGAKFMALSPDH
                     PLSQAAAKKNKNLAEFIEQCHRMGTAQAEIETAEKLGFDTGMRAIHPFDASWTLPVYV
                     ANFILMDYGTGAIFGCPAHDQRDLDFVNKYGLGNTPVVCPPGQDPKTFVITDLAYDGE
                     GTMINSRFLDGMTIEQAKEEVAKRLEKETRGNRPVAQRQVNYRLRDWGISRQRYWGCP
                     IPIIHCEKCGVVPVPENDLPVTLPDDVSFDKPGNPLDRHPTWKYVKCPKCSGPARRET
                     DTMDTFVDSSWYFARFTDPWIRTAPTDRKIVDEWLPVDQYIGGIEHAILHLLYSRFFT
                     RAMHRCGLVGIDEPFAGLFTQGMVVHETYRAKNGEWIEPACVKIEGVGDARRATIAAT
                     GEPVQIGSIEKMSKSKKNTVDPDDIIAAYGADTARWFMLSDSPPDRDVIWTEEGVQGA
                     WRFMQRLWRLIGEIAKLPAPAARPHQFGETAIKVRKVAHRALAHVSEDIDKLRFNRCV
                     AHIYEFANALGDAIGGIEKTPNDELKWALHEAGDILVQLFHPMMPHLAEECWSGLGHK
                     TLVASEAWPKVETQLLIEDTIALPVQINGKKRAEVTVSHKATNAEIEAAVLALDAVKK
                     ALGGQSPKKVIVVPQRIVNVVV"
     gene            3971..>4525
                     /gene="lptE"
                     /locus_tag="VFL49_09655"
     CDS             3971..>4525
                     /gene="lptE"
                     /locus_tag="VFL49_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET7912285.1"
                     /translation="MSWSDTRRFARIFTVLALASLTAGCFQPLYAERPTPAGVTGVAD
                     KLSGVDVASIDAPSGSRLARVSVGVRNELIYDLTGGGGSAPPSYRLNVRLSATQLQVI
                     VDINTARPDINNYGIDAVYTLTDIATGKPVAKGQTFARVSYNIPGQQQRFAGERGLRD
                     AEDRAAKEIAENIRNRLASHFAAGT"
BASE COUNT         1029 a         1293 c         1311 g          892 t
ORIGIN      
        1 gaatcgcctt ggtcagaaac aggcgcttga gtgtcttgtt cgtgtgcacg agcgacattc
       61 cgagtgtgtc agctacgtgc tgctgggtga atggaaattg gatgctcgca tccttgacca
      121 gtccagcttc ctcggcgcgc acaaaaagat gaagaagcag aaacgcggtt cgttcaagtg
      181 cggtgcgtcg accgagactg acgagatgtt cgtcgataag ttgctcttct cgcgcggcga
      241 gccaggtgat gtcgaacgcc aaagacggca ggctgctgta aagatgccac agctttgccc
      301 ggggaaaaac gcacaagacg accggcgtaa gcgcttcaac cgaatgctgc atttcacgca
      361 tcaaggtgcc ttgcaggccg accatgtcag caggaagcgc gtagttcagt atctgacgac
      421 ggccgtcatc gaggactttg taacgaaaag cccatcctct gaggacggta tacaaatggt
      481 cgctgcgcgc accttcacga agaaaactta tgccgggctc gacgcacaat tcatcgacct
      541 tgaatgtctt cacgaattca agttcttccg tagagaattc gcgcaaactc gggcgcttgc
      601 gcaactgaca ctgctcgcaa ggcgtgaatt ggccgttcga ccttccgttt ccgaggatca
      661 tgaaatctca caaacatgct tatgagaaac gctgcacgct gatgaaacga tgcttcagcc
      721 gcccaacgac gaatcttcgc gctctatgtc atttttaaat gccacccggc taaattcgta
      781 ggacaaggga aagttccttt gggagaaata gcccttgcct acgtcggata gattgttgag
      841 cggcaagcgt tgtttagtgc tggacgacga attcctgatc gccctcgaca ttcaacaaat
      901 cctggaacgg gccggtgcgg cgcatgtggc aagcgtcgcc tctgcggccg aagctattga
      961 gcttatcggg cgccagtcaa aattcgacgt tgcggtgctt gatgtgaagt tggggagtac
     1021 ggagcacaac ggcctcgacg tcgcagccct gcttcaaacg caaggcacgc catttgtatc
     1081 cctgaccggc atgcgcgtcg acgacgcgca tgccaaaaga tttcctaacg cgccggtgat
     1141 tgaaaagccc tacgatgcag cgctgctgct gagtgccgtg cagcgcgcgc tggtgcaagg
     1201 tcagaactga attgagcgca tcagatgaac gcggccgacc ggcccttggc aacggtcgat
     1261 tccttgtttt tgtgccgaac agaagggcgg cggttgaccc atcggcatct ttcgtgtctg
     1321 ttccgcgcgc cacaataaga cgcgcagatg accgccgatt cgagcaaaaa cgagcgctac
     1381 aacgcccgcg aggccgaggc ccactggcaa aaaatctggg ccgagcgcgg gatcttcgcc
     1441 accaagaatg acgacccgcg accgaaatac tatgtcctgg agatgttccc ctatccttcg
     1501 gggcgcatcc acatggggca tgtccgcaat tacacgatgg gcgacgtcgt tgcgcgcttc
     1561 aagcgcgcca aaggcaacgc ggtgctgcac ccgatgggct gggatgcgtt cggcatgccg
     1621 gcggagaacg ctgccataga tcgcaaggtg catccgaagg cctggaccta cgacaatatc
     1681 gcggcaatga aaaagcagct tcagtcgatg gggctgtcgc tggattgggc acgcgagatc
     1741 gctacctgcg acccggccta ctacacgcac cagcagaaaa tgtttctcga ctttctgaaa
     1801 gcgggcctgg tcgagcgcaa gcagtcaaag gtgaactggg atccaatcga tcagacggtc
     1861 cttgccaatg agcaagtcat tgacggccgc ggatggcgct ccggcgcatt ggttgagcag
     1921 cgcgaactca cgcaatggtt cttcaagatc agcacctact ccgaagactt gctcgcggca
     1981 ctcgatacac tcgaccgctg gccggaaaaa gtacgtctga tgcagaagaa ctggatcggc
     2041 cgctcggaag gcttgttgct gcgtttcatg ctcgatcccg caacgacacc caataaggaa
     2101 aatgaactgg aaatatttac cacgcggccc gacacgctgt tcggtgcgaa attcatggcg
     2161 ctgtcgcctg atcatccgct cagtcaggcg gcggccaaga agaataaaaa cctcgcggaa
     2221 tttatcgagc aatgccatcg catgggcacg gcgcaggccg aaatagaaac cgcagaaaag
     2281 ttaggattcg ataccggcat gagagcgatt catccgttcg atgcgagctg gacgctgccg
     2341 gtctacgttg cgaattttat tttgatggac tacggcacgg gcgcgatctt cggttgtccg
     2401 gcgcatgacc agcgcgacct cgacttcgtc aacaagtatg gactcggcaa cacgccggtc
     2461 gtttgcccgc ccggccagga tccgaaaacc ttcgtgatca ccgaccttgc ctatgatggc
     2521 gaaggcacca tgatcaactc gcgcttcctc gacggaatga cgatcgagca agcgaaagaa
     2581 gaggtggcga aacggcttga aaaagaaacg cgcggcaacc gtccggtcgc gcaacgccag
     2641 gtgaactatc gcctgcgcga ctggggaatt tcgcgccaac gctattgggg ctgtccgatc
     2701 cccatcatcc actgcgaaaa atgcggcgta gttccagtgc ccgagaacga cctcccggtc
     2761 acattgccgg acgacgttag ctttgacaaa ccgggcaacc cgctcgatcg tcacccaacc
     2821 tggaagtacg tgaagtgccc gaagtgctcc ggtccggccc ggcgcgagac cgatacgatg
     2881 gatacgtttg tcgattcgtc ctggtacttc gcgcgtttca ccgatccctg gatcagaacc
     2941 gcgcccaccg atcgcaagat cgtcgatgaa tggctgccgg tcgatcagta catcggcggc
     3001 atcgagcacg ccattttgca cctgctctac tcgcgtttct tcacccgcgc gatgcataga
     3061 tgcgggctgg tcgggatcga cgagcctttc gccggacttt ttacgcaggg catggtcgtg
     3121 cacgagacct atcgcgccaa gaacggcgag tggattgagc cggcttgtgt caagatcgaa
     3181 ggtgtcggag atgcgcgtcg cgcaactatt gccgcgaccg gcgagccggt ccaaatcggc
     3241 tctatcgaaa aaatgtcgaa gtcgaagaag aatacggtcg accctgacga catcatcgct
     3301 gcgtacggcg ccgacaccgc ccggtggttt atgctgtcgg attcgccgcc ggatcgtgat
     3361 gtgatctgga ctgaagaagg ggtgcagggc gcctggcgtt tcatgcagcg tttatggcga
     3421 ctgatcggcg agatcgccaa acttccggcc cccgccgctc ggccgcatca atttggtgaa
     3481 accgcgataa aggtacgcaa ggtcgcccat cgcgcgcttg ctcatgtttc cgaagacatc
     3541 gacaaattgc ggtttaaccg ctgcgtcgcc catatttacg aattcgccaa tgccctcggc
     3601 gacgccatcg gcggtatcga aaagactccg aacgatgagc ttaagtgggc tttgcacgaa
     3661 gccggcgaca ttctggttca gttgtttcac ccgatgatgc cgcaccttgc cgaggaatgc
     3721 tggtcgggcc tcggccacaa aactttggtc gcgagcgaag cttggccgaa ggtcgaaact
     3781 caattgctga tcgaggatac cattgcgctg ccggtgcaga taaacggcaa aaaacgggcc
     3841 gaagtgaccg tgagtcacaa ggcaacaaac gcagagatag aggctgccgt tctcgcgctc
     3901 gatgcggtaa aaaaggcgct ggggggccaa agcccgaaaa aggtcattgt cgtcccgcag
     3961 aggatcgtca atgtcgtggt ctgataccag gcgtttcgcc aggatattta cagtgctggc
     4021 cctcgccagc ctgacggccg gatgttttca gccgctttat gccgagcggc cgacgccagc
     4081 tggcgttacc ggcgttgctg acaagctgag cggagtcgac gtcgcctcaa tcgatgcgcc
     4141 gagcggaagc cggcttgccc gcgtcagcgt cggcgtccgt aacgagctga tctacgatct
     4201 caccggtggc ggaggcagcg ctccaccaag ctatcgcctg aatgtgcgcc tgagcgcaac
     4261 gcagcttcag gttatcgtcg atattaatac cgcacgccct gatatcaaca attacggcat
     4321 cgacgcggtc tatactctga ccgacattgc gaccggaaag ccggttgcca aaggccagac
     4381 cttcgcccgt gtctcctata acattccggg ccagcagcaa cggtttgccg gcgagcgcgg
     4441 actgcgcgat gcagaggatc gtgcagccaa ggaaattgcc gaaaatatcc gcaatcggct
     4501 ggcctcgcat ttcgcagcgg gcacc
//
LOCUS       DASSRL010000475         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_922588, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000475 DASSRL010000000
VERSION     DASSRL010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922588"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1251)
                     /gene="pobA"
                     /locus_tag="VFL49_09660"
     CDS             complement(85..1251)
                     /gene="pobA"
                     /locus_tag="VFL49_09660"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HET7912286.1"
                     /translation="MRTQVGIVGAGPAGLMLARLLRLQGIESIIIENRSRDYIENRIR
                     AGLIEHWAADLLADLGVGERMKREGMLHWGINIGINGDLHRIDFKALVNKRVTIYGQQ
                     EVVKDLVERRLADGGTLLFEVSDVSVHDLTGKEPKIRFRHEGRQQEINCDFIAGCDGF
                     HGICRPSIPDGVLSIFERDYPFGWLGILSENPPPDHELVYSYTDRGFALYTMRSPTLS
                     RLYLQCDHDEDIENWPDSRIWDELHKRLGGARKLQEGKMLQKGITPMRSFVAEPMQYG
                     RLFLAGDSAHIVPPTGAKGMNLAFADVMMLSRAVSAFYKDRRDDLLEKYSATCLRRVW
                     KAQRFSWWMTQIMHRFPHETAFDRRRQLSDLDYLTGSKAAATSLAEQYVGLPLD"
     gene            complement(1347..1835)
                     /locus_tag="VFL49_09665"
     CDS             complement(1347..1835)
                     /locus_tag="VFL49_09665"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7912287.1"
                     /translation="MQRTDDTAGVIAPPPLLALATIVLGLLLDWLLPAYVLTVFLSGT
                     VRIIVGVVLVGAGLALVIAANIVFRAAGTHPEPWKPSTALATDGIFAWLRNPMYVGGT
                     IFLVGLSVLLASDWMLVMTIGFFVVLHFGVVRREERYLEAKFGENYRQYCNRVPRYGW
                     PG"
BASE COUNT          445 a          623 c          584 g          424 t
ORIGIN      
        1 ctttctgatg ctggcggcaa tttccgccgc ggccggtgcg gccatcgtcg tgttgagctt
       61 tccgctgcgc tcggccctgc gggattagtc gagcggaagc ccgacatatt gttcggccaa
      121 agacgtcgcc gccgctttcg agccggtgag atagtcaaga tcggagagtt gccgccgccg
      181 atcaaaagcg gtctcgtgcg gaaagcggtg catgatctgc gtcatccacc aggaaaatcg
      241 ctgagccttc cacacgcgcc gcaagcacgt tgccgaatac ttttccagca gatcatctcg
      301 ccggtctttg taaaaagcac tgacggcgcg cgacagcatc atcacgtcgg caaaggcaag
      361 gttcattccc ttggcgccgg tgggcggcac gatatgcgcg ctgtcgcccg cgagaaacag
      421 ccggccgtat tgcatgggct cggccacgaa gctgcgcatc ggggtgatgc ccttttgcag
      481 catcttgcct tcctgcagct tgcgcgctcc gccgagccgc ttgtgcagtt cgtcccagat
      541 gcggctgtcg ggccaattct cgatgtcctc gtcgtgatcg cactgcaggt agaggcgcga
      601 caaggtcggc gagcgcatgg tgtagagggc aaagccgcga tcagtgtacg aatagacgag
      661 ctcatgatca ggcggcggat tttccgacag gattcccagc catccgaacg ggtagtcccg
      721 ctcgaatatg ctgagcacac cgtcaggaat gctcggccgg cagatgccat gaaaaccatc
      781 acagccggcg atgaaatcgc aattgatctc ctgttgccga ccttcgtgcc tgaacctgat
      841 tttgggttcc ttgccggtaa ggtcgtggac gctcacgtcg cttacttcaa acagcaatgt
      901 gccgccgtcg gccagacggc gctcgacgag atctttcacc acctcctgtt ggccgtagat
      961 cgtgacgcgc ttgttcacca acgccttgaa gtcgatgcga tgcagatcgc cgttgatgcc
     1021 gatattgatg ccccaatgca gcatgccttc gcgtttcatc cgttcgccga cacccaggtc
     1081 ggcgagaagg tccgctgccc aatgttcgat caggccggcg cgaattcgat tttcgatgta
     1141 gtcgcggctg cgattctcga tgatgatcga ttcaatgccc tgcagacgga gaagccgcgc
     1201 aagcataagc ccggctgggc ctgcgccgac gatcccgacc tgtgtgcgca tgaaacgttc
     1261 ctgcctctga tgttcctata ggcgtcagcg gccggactgg caaagggatg aagatccgaa
     1321 agcgttgcac aaaatcctga gcgccttcat cccggccagc cgtagcgcgg cacgcgattg
     1381 caatactggc ggtaattttc cccgaacttt gcctcgagat atcgctcctc gcgccgtaca
     1441 acaccgaagt gcagtacaac gaaaaacccg atggtcatca caagcatcca gtcggaggcc
     1501 agcaggacgg aaagacccac aagaaaaatt gtaccgccaa cgtacatggg attccgcagc
     1561 cacgcgaaga tcccgtccgt cgccaaagct gtagacggct tccaaggttc tggatgtgtg
     1621 ccggcggccc ggaaaacgat attggccgca ataacaagcg ccagaccggc gccgacaagc
     1681 acaacaccaa caattatccg gaccgtgccc gacaaaaaca ccgtcagtac ataggcaggc
     1741 aagagccagt ccagcagcag gcccaaaacg atcgtggcca gtgccaacag tggtggcggt
     1801 gcaatcacgc cggcggtatc gtcggtcctc tgcatagctt cggttcttta aagcggcaca
     1861 tgccgttgcc ccgggcaccc ttagcgaaaa gattaagaat taatacgact tatgcagctt
     1921 cgcagcattg actttagcgc atggcaccct atgttccgtg caaccgatca gacgtgaaga
     1981 ttcggtcgtc cccgcgcata gcaatcggtt tttccagtcc ggcgcgatct gggcgatctc
     2041 tgaggttata tggctttttc tcatctcggc ttatct
//
LOCUS       DASSRL010000476         5102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_887483, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000476 DASSRL010000000
VERSION     DASSRL010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887483"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..2705)
                     /gene="leuS"
                     /locus_tag="VFL49_09670"
     CDS             complement(99..2705)
                     /gene="leuS"
                     /locus_tag="VFL49_09670"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7912288.1"
                     /translation="MTPHRYDPREAEPRWQKLWESQGLFRAHEDDPRPKYYVLEMFPY
                     PSGRIHMGHVRNYTMGDVVARYMRARGHNVLHPMGWDAFGLPAENAAIERGVHPAKWT
                     YDNIATMRGQLKSMGLSLDWSRELATCDLDYYHQQQKLFLDLLKAGLVDRKKRKVNWD
                     PVDQTVLANEQVIDGRGWRSGALVEQREQPEWVFKITDYAEDLLDALQTLDRWPDKVR
                     LMQANWIGRSQGLLIRFALARGEAPSGHDIIEVFTTRPDTLFGASFMAIAPDHPLAKA
                     VAGRDAKAAAFIAECQHHGTSAAEIETQEKRGYDTGLRVKHPVIEGAELPVFIANFIV
                     MEYGTGAIFGCPAHDQRDLEFARAYGLPVIPVVLPPDADAKSFTIGEEAYTGDGTLIN
                     SGFLDGLTVEAAKDAMADHLATKMLHGAPQGERRTEFRLRDWGISRQRYWGCPIPMIH
                     CETCGIVPVPEKDLPVKLPEDVTFDGTGNPLDRHPTWKHVTCPSCGGPATRETDTMDT
                     FVDSSWYFARFCSPHAQVPVDSNAVGYWLPVDQYIGGVEHAILHLLYARFFTRAMHKT
                     GHSPIDEPFKSLFTQGMVTHETYKDAHGKWVTPKEVAYREGLPVLAATGEPIAIGPIE
                     SMSKSKKNVVDPDDIIAAYGADTARWFMLSDTPPERDIEWTAAGVDGAYRFLQRVWRL
                     VNEAAEKGEGGQASAEMGSDALQLRRAAHRSAAAVTAAIDKLRFNVAIAHIYELANAL
                     SAALNAAGEKPVQDMRFALREAAEMLTRIAAPMVPHLAEECWLALGNQGLVAEQPWPA
                     PDPALLAEDTVVIAVQVNGKRRDELRIARDAAKEDIEAAALKLDNVVRAIGGRRIKKV
                     VVVPQRIVNVVA"
     gene            2810..3805
                     /locus_tag="VFL49_09675"
     CDS             2810..3805
                     /locus_tag="VFL49_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7912289.1"
                     /translation="MRKVSSMIAEKQARLQTCSRPMWRALILGCVVACISAALSIPAG
                     AESDQSLAWCKGDADPTLEQQIGACTALIEAKSGEARTRALNYFRRAGAYLQQQDFDK
                     AISDFGDGLAIDAGNAAAYYGRAIAYEAKKDTDRAIADYDAAIKFDPDNVKALSNRAA
                     IYADMRAYERALEDNNRVVELAPDQAAGYVGRGLTYARRGDFEYALRDFDRAIALDAK
                     SAIAYFNRGRVYFSKGDVERAIEDYGAAIDIGPKNASAFENRGFAYLSQGQIDKAIAD
                     YNAALELDAKRATALYGRGTAKLKKGDTSGADDIAAAKAIKPDIAGVFEQSGVQR"
     gene            3802..4491
                     /locus_tag="VFL49_09680"
     CDS             3802..4491
                     /locus_tag="VFL49_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7912290.1"
                     /translation="MIDDARVTSSLHAVKRDIADAAHRAGRDPYSVKLIAVSKTVPEH
                     AIEEAIGLGQHRFGENRVQEAKAKWPALKERHPDVELHLIGPLQSNKVRDAVQLFDVI
                     HSVDRPKIAAALAEEMKRVGKYPRLLVQVNTGEEPQKAGVLPSETDAFVARCRDDYGL
                     AIEGLMCIPPLDEDPAMHFALLGKIAARLGLNELSMGMSGDFAKAIAFGATYVRVGTA
                     IFGARKAAPQN"
     gene            complement(4488..4775)
                     /locus_tag="VFL49_09685"
     CDS             complement(4488..4775)
                     /locus_tag="VFL49_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7912291.1"
                     /translation="MSVRYTARALGQLAEIFDYIAQDNPRAAHAVQLRIKLSFERLDD
                     FPFAGRITDRSGVRVLTVVRYPYRIFYRTSGAGDVLILRILHAARDSSRDA"
     gene            complement(4772..5023)
                     /locus_tag="VFL49_09690"
     CDS             complement(4772..5023)
                     /locus_tag="VFL49_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912292.1"
                     /translation="MRACYSVMMTKLLDKALERVRSWSRDRQDDAARLLLAMDRHDAE
                     PYKLTPQERDEIDAALAKAERGELASDDEVAALFKRFGV"
BASE COUNT          975 a         1638 c         1568 g          921 t
ORIGIN      
        1 ccaagcgcga gccgccggcg gtcgtgccgt aaagcggctg gatgccgcag ccggccaagc
       61 taatggccgc ggtcacagcc aaagcggcaa gcaatggcct atgccaccac attgacgatc
      121 ctctgcggta cgaccacaac cttcttgatt cgacgtcccc caatggcgcg cacgacattg
      181 tcgagcttta gcgcagcagc ttcaatatct tccttcgctg catcgcgcgc aatcctcaat
      241 tcatcccgtc tttttccgtt cacctgtacc gcaattacca cggtatcctc ggccaaaagg
      301 gccggatccg gggccggcca gggctgttcg gccacgaggc cctgattccc cagggccaac
      361 cagcattctt cagccagatg cggcaccatt ggggcggcaa tccgggtgag catttccgca
      421 gcttcccgaa gcgcgaatcg catgtcctgg acgggtttct cgccggcggc gttgagggcc
      481 gccgaaagcg cgttggcaag ctcgtaaata tgggcaatgg cgacgttgaa gcggagcttg
      541 tcgatcgcag cagtgaccgc agcggcgctg cgatgcgccg cccggcgaag ctgtaacgcg
      601 tcgcttccca tctccgccga agcctggccg ccctccccct tctccgcagc ctcgttgacc
      661 agccgccaca cccgctgaag gaaccggtaa gcgccgtcga cgccggccgc ggtccactcg
      721 atgtcgcgct caggcggcgt gtccgacagc atgaaccagc gcgccgtgtc ggcgccgtag
      781 gcggcgatga tatcgtcggg atcgaccacg ttctttttcg acttcgacat cgattcgatc
      841 ggaccaatcg cgatcggctc tccggtcgcg gccagcacgg gcaatccctc gcgataggcc
      901 acctctttcg gcgtcaccca tttgccgtgc gcgtccttgt aggtctcgtg cgtcaccatg
      961 ccttgagtga acaggctctt gaacggctcg tcgatcggcg aatgcccggt cttgtgcatg
     1021 gcgcgggtga agaaacgcgc atagaggaga tgcaggatcg catgctcaac gccgccgata
     1081 tattggtcga ccggcagcca gtagccgacg gcgttcgaat cgaccggcac ttgcgcatgc
     1141 ggcgagcaga agcgagcgaa gtaccaggac gaatcgacga aggtgtccat ggtatcggtt
     1201 tcgcgcgtgg cgggaccgcc gcagctcggg caggtcacat gcttccaggt gggatggcgg
     1261 tcgagcggat tgccggtgcc gtcgaaggtc acgtcttcag gcagcttcac cggcaggtcc
     1321 ttctcgggca ccggcacgat gccgcatgtc tcgcagtgga tcatcgggat cgggcaaccc
     1381 cagtagcgct gccgcgagat gccccaatcg cgcaagcgga actcggtgcg tcgctcgcct
     1441 tgcggcgcgc cgtgcagcat cttcgttgcc agatgatcgg ccatcgcgtc ttttgccgcc
     1501 tcgactgtga ggccgtcgag gaagccggaa ttgatcagcg tgccgtcgcc ggtataggcc
     1561 tcttcgccaa tcgtgaaact cttggcgtct gcgtcgggcg gcagcaccac cgggatcaca
     1621 ggaagcccat aggcgcgcgc aaattcaagg tcgcgctgat cgtgcgccgg acagccgaag
     1681 atggcgccgg tgccgtactc catgacgatg aaattggcga tgaacacggg aagctcggcg
     1741 ccctcgatca ctgggtgctt tacgcgcaag ccggtgtcgt atcccctctt ctcctgcgtc
     1801 tcgatctctg ccgcgctcgt gccgtggtgc tggcattcgg cgatgaaagc tgcggccttc
     1861 gcgtcacgcc ccgccacggc cttggccaga ggatgatccg gcgcgatcgc catgaagctt
     1921 gcgccgaaca gcgtatctgg ccgcgtggtg aatacttcga tgatgtcgtg gccggacggc
     1981 gcctcacctc ttgccaacgc gaagcggatc agcagccctt gcgatcgtcc gatccaattc
     2041 gcctgcatca gccgcacctt gtcgggccag cggtcgagcg tttgcagcgc atcgagcagg
     2101 tcttccgcgt agtcggtgat cttgaacacc cattcaggct gctcgcgctg ctcgaccagc
     2161 gcgccggagc gccagccgcg cccgtcgatc acctgctcgt tggcgagcac ggtctgatcg
     2221 accgggtccc aattcacttt gcgcttcttg cggtcgacaa ggcctgcctt gagcagatcg
     2281 agaaacagct tctgctgctg gtggtaatag tcgagatcgc aggtcgcgag ctcgcgtgac
     2341 cagtcgagcg acagccccat gctcttgagc tggccgcgca tggtggcgat gttgtcgtag
     2401 gtccatttgg cgggatggac gccacgctcg atcgccgcgt tctccgccgg aaggccaaat
     2461 gcatcccagc ccatggggtg aagcacgtta tgcccgcggg cgcgcatgta gcgggcaacc
     2521 acgtcgccca tggtgtaatt gcgcacgtgt cccatatgga tgcgcccgga ggggtatggg
     2581 aacatttcaa gaacatagta cttcgggcgc gggtcatcct catgggcgcg gaacagccct
     2641 tggctctccc agagcttctg ccagcgtggc tccgcctcgc gcggatcgta gcgatgtggg
     2701 gtcatggtct gctgttgtgg tccgaatttg cgccgactag gccatgaaag ccgaagctcg
     2761 gtcaatctag ccgggacaac gacagcgtga tttgccgcaa ggccttggca tgcgcaaagt
     2821 gtcgtccatg atcgccgaga aacaagccag actgcaaact tgctcccgcc cgatgtggcg
     2881 cgcgctcatt ctgggttgcg tggtggcatg catatccgct gccttgtcga ttccggccgg
     2941 ggctgagagc gaccagagcc tcgcctggtg caagggagac gccgacccca ccctagagca
     3001 acagatcgga gcctgcaccg cgttgatcga ggccaagtcc ggcgaggctc gaacgcgtgc
     3061 gctcaactat tttcgccgtg ccggcgccta tctccagcaa caggacttcg acaaagcgat
     3121 cagcgatttc ggcgacggcc tcgcgatcga tgccggcaac gccgcagcct attatggccg
     3181 cgccatcgcc tacgaagcaa agaaggacac cgaccgcgcc attgcggact acgacgccgc
     3241 gatcaagttc gatccggaca acgtcaaggc gctgagcaac cgcgccgcga tctacgccga
     3301 catgcgcgcc tatgagcgcg cgctcgagga caacaaccgc gtcgtcgagc tcgcgcctga
     3361 tcaagcggcg ggatacgtcg gccgtgggct cacttatgcg cgccgcggcg acttcgaata
     3421 tgcactccgc gacttcgacc gcgccattgc gctcgatgcc aagtcggcca tcgcctattt
     3481 caatcgtggc cgcgtctatt tcagcaaggg cgacgtcgag cgcgcgatcg aggactatgg
     3541 cgccgccatc gatatcggtc cgaagaatgc cagcgccttc gagaaccgcg gcttcgccta
     3601 tcttagccaa ggccagatcg acaaggcgat cgccgactac aacgcggccc ttgagctcga
     3661 cgccaagcgc gccacggcgc tttacgggcg cggcaccgcc aagctgaaaa agggcgatac
     3721 cagcggggcc gacgacatcg ccgccgccaa ggcgatcaag ccggacatcg ccggcgtgtt
     3781 cgagcagtca ggcgtgcaac ggtgatagac gacgctcgag tcacctcctc gctgcacgca
     3841 gtgaagcgcg atatcgccga tgccgcgcac cgcgccggcc gcgatcctta ctcggtcaag
     3901 ctgatcgccg tcagcaagac cgtgcctgag cacgctatcg aagaagccat tgggttaggg
     3961 cagcaccgct tcggcgaaaa ccgcgtgcag gaagcgaaag cgaagtggcc ggcgctgaag
     4021 gaacggcatc cggatgttga gcttcatctc atcggcccgc tgcaatcgaa caaggtgcgt
     4081 gacgcggtgc aactgttcga cgtgatccac agcgtcgacc gaccgaagat cgcggcggcg
     4141 cttgccgagg agatgaagcg cgttggtaaa tacccgcgcc tgctcgtgca ggtgaatacc
     4201 ggcgaggagc cgcagaaggc cggcgtgctt ccgagcgaga cggacgcatt tgtggcgcgc
     4261 tgccgcgacg attatggtct cgcgatcgaa ggcctgatgt gcatcccgcc gcttgacgag
     4321 gaccctgcca tgcacttcgc gttgctcggc aagatcgctg cgcgcctcgg cctcaacgag
     4381 ctgagcatgg gcatgagcgg cgactttgcc aaggccatcg ccttcggcgc cacctatgtg
     4441 cgcgtcggta cggcaatctt cggcgcgcgc aaggccgcac ctcagaacta ggcatcacgc
     4501 gatgagtcgc gtgcggcgtg gaggatacgc aatatcagaa cgtcgcccgc gccggaggtg
     4561 cgataaaaga ttcgataggg ataacgaaca acggtcaaaa cgcgaactcc ggaacgatca
     4621 gtaatgcgac cagcaaatgg gaagtcgtcc aaccgctcaa acgagagctt gatccgcagc
     4681 tggaccgcgt gcgcggcacg aggattatcc tgcgcgatat agtcgaaaat ctcagcaagc
     4741 tgcccgaggg cccgcgcggt gtagcgaacg ctcatacacc gaagcgcttg aacagggcag
     4801 cgacttcatc atcgcttgcc aattcgccgc gctcggcttt tgccaacgct gcgtcgattt
     4861 cgtcacgttc ttgcggtgtg agcttgtacg gctcggcatc gtgacgatcc atggcaagca
     4921 acaatcgcgc agcatcgtcc tgtcgatccc tcgaccatga gcggacgcgt tcaagagcct
     4981 tgtcgagcag cttcgtcatc atcacactgt agcacgctct cattgctaca tcaccacacc
     5041 tacatcacca caatcgcaga gccgcgccgc aactgcgaca gcaacatcag tagatcgtgc
     5101 cg
//
LOCUS       DASSRL010000477         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_47748, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000477 DASSRL010000000
VERSION     DASSRL010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47748"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFL49_09695"
     CDS             complement(<1..357)
                     /locus_tag="VFL49_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7912293.1"
                     /translation="MRGKDNARSGGEILIDQLAIHGVRDVFCVPGESYLAALDAFHDS
                     DIKLTVCRHESTAAIMAEAVGKTTGRPGVCFVTRGPGASNASAGIHIARQDSSPMIIF
                     VGQIGRDMRDREAFQEL"
     gene            complement(363..704)
                     /locus_tag="VFL49_09700"
     CDS             complement(363..704)
                     /locus_tag="VFL49_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET7912294.1"
                     /translation="MIIDHRVYRTLPGRLPAQLELYGKLGYPVQLRYMGEPHYYLATE
                     TGQLNTLVHGWIYESAAQREQTRAKMAQDPDWKNYLAENAKAGHIIEQTNWLMTPVPF
                     SPKIPPAKIVK"
     gene            complement(775..2616)
                     /locus_tag="VFL49_09705"
     CDS             complement(775..2616)
                     /locus_tag="VFL49_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7912295.1"
                     /translation="MTSRIEDYAMIGDLGTAALIGRDGSVDWLCWPRFDSDACFAALL
                     GKPEHGRWLIAPKSTSMRVTRRYRPNTLILETRFESADGAATLIDFMPPRSAHAHLIR
                     IVKGERGRVAFNIELILRFAYGAIVPWVTRIDDITVRAIAGPDMAVVRTPVRMRGENF
                     KTVGEFTVAPGEEVPFALSFARSHQDVPEPIDAFECLAATEKFWAEWASRNKIQCPWR
                     EAVTRSLIVLKSLTYAPTGGMAAAPTTSLPECIGGSRNWDYRYCWLRDATLTLLALMN
                     AGYYDEAKVWREWLLRAAAGSPRQIQIMYGLRGERRLTEWQVPWLPGYENSKPVRIGN
                     AAHTQLQLDIFGEVMDALHQARQGGLGAHEAGWAMQREFLLHLARIWHEPDEGLWEVR
                     SGREHFTYSKVMAWLAFDRAVRSAEKFKLPGPIEQWREIRERIHADVCAHGFNTNLGT
                     FVRSYGSKELDASLLLLPAIGFLPPDDPRIVATIAAIERRLLVKGLVLRYDSATAKDG
                     LPAGEGVFLACSFWLADAYLMLGRREEALHLFNYLLSLRNDVGLLSEEYDPRNGRLVG
                     NFPQAFSHLALVNTASNLAHYQKPAEQRTQHRVLDKPSMQPDKPEVY"
     gene            complement(2626..>3059)
                     /locus_tag="VFL49_09710"
     CDS             complement(2626..>3059)
                     /locus_tag="VFL49_09710"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912296.1"
                     /translation="DRAQSGPKPALVQSLGATYHTGSLGNLCPDIVIECTGAGSVVVD
                     VIGRTAANGIVCLAGVSSGGHKIQFDIGDLNRTMVLENEMVFGSVNANRRHYRGAAEA
                     LIRAERRWLTGLITRRVPLTRWQEAFEHRDNDIKVVVDFTA"
BASE COUNT          608 a          973 c          913 g          565 t
ORIGIN      
        1 gagctcctgg aatgcttcgc ggtcgcgcat gtcgcgccca atctggccga cgaatatgat
       61 catcggcgag gaatcctggc gcgcgatatg aatgcctgcc gacgcgttgc tggcgccggg
      121 cccgcgcgtg acaaagcaca cgccgggccg gccggtcgtc ttgccaaccg cctccgccat
      181 gatggccgca gtgctctcat ggcggcacac ggtgagcttg atatcgctat catggaacgc
      241 atcgagcgcg gcaagataac tttcacccgg cacgcaaaac acgtcgcgca ccccgtgaat
      301 cgccaactga tcgatcagga tttcgccgcc ggaacgcgcg ttgtccttgc cgcgcatcga
      361 agttacttca cgatctttgc gggaggaatc ttcggcgaga aaggaacagg cgtcatcagc
      421 caattggtct gctcgataat gtgtcccgct ttcgcgtttt ccgccaggta gttcttccag
      481 tccggatcct gcgccatctt ggcgcgcgtt tgctcacgct gagcggcact ctcatagatc
      541 catccatgca ccagcgtatt gagctgaccg gtctcggtcg cgagatagta gtgcggctca
      601 cccatgtagc gaagctgcac cggatagccc agcttgccgt aaagctcgag ctgtgcgggc
      661 agcctgcccg gcagagtccg ataaaccctg tgatcgataa tcactaaaca cctccttgga
      721 aagcagccgc ctggcgcggt caggcgcgca ctttccgggc aaggcgacca gcggtcaata
      781 gacttccggc ttgtccggct gcatggacgg cttgtctaga acgcggtgct gcgtgcgctg
      841 ctccgccggc ttttggtagt gcgcaagatt gcttgcagtg ttgaccagtg cgagatgcga
      901 aaaggcctgc ggaaaattgc cgaccagccg accgttgcgc ggatcatact cttcgctcag
      961 gagtccaaca tcgttgcgca gcgagagaag atagttgaac agatgcaacg cttcctcgcg
     1021 acggcccagc atcagatagg cgtctgcgag ccagaagctg cacgccagga acacgccctc
     1081 gccggccggc agaccgtctt tcgctgtcgc gctgtcgtaa cggagcacca ggcctttgac
     1141 caacaggcgg cgttcgatcg ccgcgatggt ggcgacgata cgcggatcgt cgggaggcag
     1201 aaatccgatc gccggcagca acagcaggct ggcgtcgagc tccttggagc cataggaacg
     1261 gacgaaggtt ccaagatttg tgttgaaccc gtgcgcgcac acgtcggcat ggatgcgctc
     1321 gcgaatttca cgccattgct cgatcggacc cggcagcttg aacttttccg cacttcggac
     1381 ggcacgatcg aaggcgagcc aggccataac cttggagtac gtgaaatgct cgcggccgct
     1441 gcgcacctcc cacagccctt cgtccggctc gtgccagatt cgcgcaagat gcagcaaaaa
     1501 ttcgcgctgc atcgcccagc cggcctcgtg cgcgccgaga ccgccttgcc gcgcctgatg
     1561 cagcgcgtcc atcacctcgc cgaaaatatc aagctggagc tgtgtatgcg ccgcgttgcc
     1621 gatacgaacc ggcttcgaat tctcatatcc cggcagccaa gggacctgcc actcggtcag
     1681 tcgccgttcg ccgcgcaggc catacatgat ctgaatctga cgcggactgc cggccgccgc
     1741 gcgcaacagc cattcgcgcc acaccttcgc ttcgtcgtag tagcccgcat tcatgagtgc
     1801 gagcagcgtg agcgtggcat cgcgcagcca gcaatagcga taatcccaat tgcgcgaacc
     1861 accaatgcat tccggcaggg atgtcgtggg cgccgccgcc atgcccccgg tcggagcgta
     1921 agtgagcgat ttgagcacga tcagcgaccg cgtcacggct tcacgccaag ggcactggat
     1981 cttgttgcgg ctagcccact ccgcccaaaa cttctccgtc gccgccaggc actcgaaggc
     2041 atcgatcggc tccgggacgt cctgatgcga tcgcgcgaaa gagagcgcaa aaggcacttc
     2101 ttctcctggc gcgacggtga attcgccgac cgtcttgaag ttttcgccgc gcatgcgaac
     2161 cggcgtgcgt accacggcca tatcgggccc tgctatggct cgcacggtga tatcgtcgat
     2221 ccgcgtgacc cagggcacga tcgcgccgta ggcaaagcgc aggatgagct cgatgttgaa
     2281 tgcaacccgg ccacgctcgc ctttgacgat ccggataaga tgcgcatggg cgctgcgcgg
     2341 gggcatgaag tcgatcaagg ttgccgcgcc atcggctgac tcaaaacggg tctccagaat
     2401 caaggtattc ggccgatagc ggcgcgtgac gcgcatggag gtcgatttcg gcgcgatcag
     2461 ccagcgtccg tgctcgggtt tgcccaacag cgccgcaaag caggcatccg aatcgaaacg
     2521 cggccagcag agccagtcca ccgagccatc gcgcccgatc agcgcggcgg tgccgaggtc
     2581 gccgatcatt gcatagtctt caatgcgcga cgtcatgccg ccccgctatg ccgtgaagtc
     2641 gacgacgacc ttaatgtcgt tgtcccgatg ctcgaaagct tcctgccacc gcgtcaacgg
     2701 cacgcgccga gtaatcaagc ccgtaagcca gcggcgttcg gcccggatga gtgcttctgc
     2761 ggcaccgcga tagtgccggc ggttggcatt gaccgatcca aataccatct cgttctccag
     2821 aaccattgtg cgattaagat cgccgatgtc gaactgaatc ttgtgaccgc ccgatgagac
     2881 gcctgcgagg caaacaattc cgttggccgc cgtcctgccg atgacgtcaa cgacaaccga
     2941 gccggctccg gtgcattcga tgacaatatc gggacaaagg ttccccaacg agcccgtgtg
     3001 gtaggtggcg ccgagcgatt gcacgagagc aggcttggga ccactttgcg cacgatcga
//
LOCUS       DASSRL010000478         5801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_240322, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000478 DASSRL010000000
VERSION     DASSRL010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240322"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFL49_09715"
     CDS             complement(<1..631)
                     /locus_tag="VFL49_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HET7912297.1"
                     /translation="MASNIKSKPISKESPTEPFKRAVTGCLRALARKPELEVAFAAER
                     PGLMGGKVRLPEPPRKLGKAEAAIVRGHADSIALRLACHDSAMHRRLQPSGQQARAVF
                     EAVEQARVEAIGARRMDGVAQNLTAMLDERFHRGKFDEITERADAPIEDALALMVRER
                     LTGLAPPPAARRVVDLWRPLIEDRAGGDLDRLESLVEDQRQFSDVVHDLL"
     gene            complement(752..1738)
                     /gene="cobS"
                     /locus_tag="VFL49_09720"
     CDS             complement(752..1738)
                     /gene="cobS"
                     /locus_tag="VFL49_09720"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HET7912298.1"
                     /translation="MTAAMQESAGLPDMKVSVRQVFGIDSDLEVPAYSRADEHVPDID
                     SDYRFDRPTTLAILAGFAKNRRVIVTGYHGTGKSTHIEQVAARLNWPCVRVNLDSHIS
                     RIDLIGKDAIVIKDGQQVTEFREGILPWALQNNVALCFDEYDAGRPDVMFVIQRVLEA
                     SGRLTLLDQSKVIKPHPAFRLFATANTVGLGDTSGLYHGTQQINQGQMDRWSIVTTLN
                     YLPHDNEVEIVLAKAKHYRNEQGRDIVNKMVRVADLTRNAFMNGDISTVMSPRTVITW
                     AENSEIFNDIGFAFRLTFLNKCDELERPIVAEFYQRCFGVELPESSVNVALS"
     gene            complement(1944..2543)
                     /locus_tag="VFL49_09725"
     CDS             complement(1944..2543)
                     /locus_tag="VFL49_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET7912299.1"
                     /translation="MKFDSPLFDRIRVKPEKDRRPKTGGPGCEWHGCKEKATHRAPKG
                     RGREKDYWRFCLDHVREYNQAYNFFAGMSDAAVMAYQKDALTGHRPTWKMGTGKGTVR
                     PDLNAFGSQADPFGLFGEGVRAEQKARAETRPIRNAERKALDTLGLDVGATSQQVKMR
                     FKELVKRHHPDANGGDRSTEDRLVEIIQSYNYLKSVGFG"
     gene            2633..2908
                     /locus_tag="VFL49_09730"
     CDS             2633..2908
                     /locus_tag="VFL49_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET7912300.1"
                     /translation="MRTADLITQKLTETFAPQSLEVVDESHRHEGHAGHRPGGQTHYR
                     VYIVSDAFKGKSRIERHRMINQSLAAELTGGVHALAIHASAPGEGGS"
     gene            complement(2905..4194)
                     /locus_tag="VFL49_09735"
     CDS             complement(2905..4194)
                     /locus_tag="VFL49_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET7912301.1"
                     /translation="MTTNDWLSLVVVLICLLLSAFFSGSETALTASSRASMARLDKHG
                     NRRAAIVNKLLAQRERLIGALLLGNNVANIAASALATGVLLAWFGNAGVVYATALMTV
                     LVVVFAEVLPKTAAFNAPDRIALAVAKPMEWVVRLLGPILIWIEKLVCWMLARFGMDV
                     EEDQAVLSAREELRGAVDLLHREGDVERLDRDMFGGVLDLRELAVSDVMIHRTNMITL
                     DADDSSEDVVNAVIASPVTRLPMWRGNPENIVGILHVKDLLRALHAVDGDAAKVDIAS
                     LMTPPWFVPDTRPVSEQLKAFRRRKTPFALVVDEYGEVEGLVTLEDILEEIVGDITDE
                     HDVAMPGVRRQPDGSVNVDGAVPIRDLNRVMDWNLPDEEATTIAGLVIHEARSIPEVG
                     QSFTFHGFRFRVLRRARNRITALRIQPLLRTQPAKAG"
     gene            complement(4198..5325)
                     /gene="aroB"
                     /locus_tag="VFL49_09740"
     CDS             complement(4198..5325)
                     /gene="aroB"
                     /locus_tag="VFL49_09740"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7912302.1"
                     /translation="MTAPLRQSEPIVVPVALGERTYDIFIGRGLLASLGTRIKNLRSA
                     ARVAIVTDEAVAAHHLAATEASLGAAGIDCARIVVPAGESSKNYATFETVCEAIIAAR
                     VERNDLVVALGGGVVGDLAGFAAACARRGLDFVQVPTTLLAQVDSSVGGKTGINSRQG
                     KNLVGAFHQPVLVVADSALLDTLPKREFRAGYAEVAKYGLLGDAAFFAWLEKNWQDVF
                     AGGPAREHAIAVCCRAKAGIVARDERETGERALLNLGHTFGHALEAGCGFSDRLLHGE
                     AVALGMALAFEFSAKKGLIVASDAARASSHLAAAGLPTHVKDVPGGVPPVDRLMELIA
                     QDKKVKRGALTFILVRGIGRAFIEKNVDAAEVHAFLTDKLK"
     gene            complement(5322..>5801)
                     /locus_tag="VFL49_09745"
     CDS             complement(5322..>5801)
                     /locus_tag="VFL49_09745"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7912303.1"
                     /translation="GKSSIGRRLAGRLGIPFVDADTEIESAAGMTIPEIFEKHGEPYF
                     RAGEARVIARLLDNGPQVLATGGGSVMDAQTRALIGEKGISIWLKADIDVLLKRTKRR
                     NDRPLVERIRDLLPLREPLYAQAHIIVQSRDEPHDTIIDEIMGELRKRLELGAEKSS"
BASE COUNT         1074 a         1875 c         1776 g         1076 t
ORIGIN      
        1 cgagcagatc gtgcacgacg tcgctgaatt gccgctggtc ctcgaccagg ctttcaagcc
       61 ggtcgaggtc accgccggcg cggtcctcga tcagcgggcg ccacagatcg accacacgcc
      121 ttgcggcggg cggcggtgca agaccggtaa ggcgctcgcg caccataagt gccagcgcgt
      181 cctcgatcgg cgcgtccgcg cgttcggtaa tctcgtcgaa ttttccgcgg tggaagcgtt
      241 cgtcgagcat cgcggtgagg ttctgcgcga cgccatccat gcgccgtgcg ccaatcgcct
      301 cgacgcgcgc ctgctcgacc gcctcaaaca ccgcacgcgc ctgctggccg gacggctgca
      361 gacggcgatg catcgcggaa tcgtggcatg caagcctcag cgcgatggag tcggcatgac
      421 cgcgcacgat ggcagcctcc gccttgccaa gcttgcgcgg cggctcaggc aggcgaactt
      481 tcccgcccat cagtccggga cgctcggcgg caaacgcgac ctcgagctcc ggcttgcgcg
      541 cgagcgcacg cagacaaccg gtaaccgcgc gcttgaaggg ctcggtcggc gattccttcg
      601 atatcggctt gctcttgatg ttggaggcca tctttttatc tgtcgttccg gagcgcgcgc
      661 agcggcgagc ccggaaccca tactcacaga cctctccgca taatcgcaga caccggggtt
      721 atgggtcccc gctttgcgga gacgacaaaa gtcagctcag cgccacgttc accgacgatt
      781 ccggcaactc gacgccgaag cagcgttgat aaaactccgc gacgatcggg cgctccagct
      841 catcgcattt gttcaggaag gtgagcctga aggcgaatcc gatatcgttg aagatctcgg
      901 aattttccgc ccaggtgatc accgtgcgcg ggctcattac ggtcgaaatg tcgccgttca
      961 tgaaggcgtt gcgtgtgagg tcggcgacgc gcaccatctt gttgacgatg tcgcggccct
     1021 gctcgttgcg atagtgcttc gccttggcaa gcacgatctc cacttcattg tcgtgcggca
     1081 gatagttgag cgtggtgacg atcgaccagc ggtccatctg accctggttg atctgctgcg
     1141 tgccgtgata gaggcccgac gtgtcaccga ggccgaccgt gttggcagtc gcaaacaggc
     1201 ggaacgccgg atgcggcttg atcaccttgc tctggtcgag cagcgtgagc cggcccgatg
     1261 cctccagcac gcgctggatg acgaacatca cgtccggccg gccggcatcg tactcatcga
     1321 agcagagcgc gacattgttc tgcagcgccc atggcagaat gccctcacgg aattcggtca
     1381 cctgctggcc gtccttgatc acgatcgcat ctttgccgat caggtcgatg cggctgatgt
     1441 ggctgtcgag attgacgcgc acgcatggcc agttgagccg ggccgcaacc tgctcgatat
     1501 gggtcgattt gccagtgccg tgatacccgg tgacaatgac gcggcggttc ttcgcaaaac
     1561 ccgcaagaat ggcaagcgtg gtcgggcggt cgaaacggta atcgctgtcg atgtcgggta
     1621 cgtgctcatc agccctggaa taggcgggca cttccagatc gctgtcgatc ccgaaaacct
     1681 gccgaactga cactttcatg tcgggcaggc cggcactttc ttgcattgcg gcggtcattg
     1741 atcctccgtg gcttcggaac gccgaagccc gtgatgcggt ccgattcgta cggtccggct
     1801 gatggcggcg gcaacttagc agaggccacg gcggcaaaaa acccggccgt cccgaatggc
     1861 accatcaatt atttcaataa gataggtgct gacagccctg cctgaaaggc ggggctgggt
     1921 tgtctgcggc aatccgctgc cgatcagcca aaccccaccg atttaaggta gttgtacgac
     1981 tggataatct cgaccagacg gtcttcggtg gaacggtcgc cgccgttcgc gtcgggatgg
     2041 tggcgcttta ccaattcctt gaaacgcatc ttcacctgtt gcgaggtcgc gccgacatcg
     2101 agacccagcg tgtcgagcgc cttgcgttcg gcattacgga tcggacgcgt ctccgcacgg
     2161 gctttctgct cggcccgcac gccttcgccg aacaagccga atggatccgc ctgcgaaccg
     2221 aacgcgttca ggtcaggacg cactgttccc ttgccggtcc ccattttcca ggtgggccgg
     2281 tggccggtga gcgcgtcttt ctgataggcc atcacggccg catcgctcat acccgcgaag
     2341 aaattatatg cctggttgta ctcgcgcaca tgatcgagac agaaacgcca gtagtccttc
     2401 tcgcggccgc ggcctttcgg cgcgcgatgt gtcgcttttt ccttgcagcc gtgccactcg
     2461 cagccgggcc cgcccgtctt cggacggcgg tccttctccg gcttgacgcg gattcgatca
     2521 aacagaggcg agtcgaattt catgaccgaa cattatgcgc tcttgcaggt gacgcgttca
     2581 agacttgttt gcggctattg accggcaggc cgcactctcc taagcgggcg ttatgcggac
     2641 cgccgatctc atcacgcaaa agttgaccga gactttcgcc ccgcaaagcc tcgaggtggt
     2701 cgatgaatcg caccggcatg aaggtcatgc cggacaccgt ccgggcgggc aaactcacta
     2761 ccgggtctat atcgtgtcgg atgcgttcaa gggaaagagc cgtatcgaac gccaccgcat
     2821 gattaatcaa tcgcttgccg ccgaactcac gggcggcgtg cacgcgctcg ccatccatgc
     2881 atccgcgccc ggcgagggcg gctcctagcc ggctttcgcc ggttgcgtcc gcagcaaagg
     2941 ctggatgcga agcgcagtga tgcggttgcg cgcgcggcgc aggacccgga agcggaatcc
     3001 gtgaaaagtg aaactctgtc ccacttccgg gatcgaacgc gcctcatgaa tgacaagacc
     3061 ggcgatggtc gtcgcctctt cgtcgggcaa attccagtcc atcacgcggt tgaggtcgcg
     3121 gatcggcaca gcaccgtcga cgttcaccga tccgtccggc tggcgtcgga cacccggcat
     3181 cgccacgtca tgctcgtcgg tgatatcgcc gacgatctcc tcgaggatgt cctcgagcgt
     3241 caccaaccct tccacttcgc cgtactcgtc gaccaccaag gcgaaaggcg tcttgcgccg
     3301 gcggaaggcc ttgagctgct ccgataccgg acgcgtgtcc ggcacaaacc agggtggcgt
     3361 catcagcgag gcaatgtcga ccttcgccgc atccccgtcc acggcatgca atgcacgcaa
     3421 caaatccttc acatgcagaa taccgacgat gttctcagga ttgccgcgcc acatcggcag
     3481 gcgtgtcacc ggcgaggcta tcaccgcatt gacaacgtcc tcggatgaat cgtccgcatc
     3541 gagcgtgatc atattggtgc gatgaatcat cacgtcggag acagcgagtt cgcgcagatc
     3601 gagcacgcca ccgaacatgt cgcggtcaag tcgctccacg tcgccctcgc gatgcaggag
     3661 gtcgacggcc ccgcgcaatt cctcgcgggc ggaaaggacc gcctggtctt cttctacgtc
     3721 catgccgaag cgcgcgagca tccagcagac aagcttttcg atccagatca gaatcggacc
     3781 gagcaggcgc accacccatt ccatgggctt ggcgacggcg agtgcgatac ggtcgggtgc
     3841 gttgaaggcc gcggttttcg gcagcacctc ggcgaacacg acgaccagca cagtcatcaa
     3901 cgcggtggcg tagaccacgc ctgcgttgcc gaaccacgcg agcagcacgc ccgtcgccaa
     3961 tgccgacgcg gcgatattgg ccacattgtt gccgagcagc agcgcgccga tcaggcgttc
     4021 gcgctgcgcc agcaacttgt tgacgatggc cgcgcggcgg tttccatgct tgtccaggcg
     4081 cgccatgctc gcgcgcgacg acgcggtcag cgccgtttca ctccccgaga aaaaggcgga
     4141 aagcagcagg cagatcagga cgacgacaag tgacagccag tcgttcgtgg tcatcgatta
     4201 cttgagcttg tccgtgagga acgcgtggac ttcggcggca tcgacatttt tctcgatgaa
     4261 agcccggccg atgccgcgca ccagaatgaa tgtgagcgcg ccgcgcttca cttttttgtc
     4321 ctgggcgatc agctccatca acctgtcgac gggcggaacg ccgccgggaa cgtccttaac
     4381 gtgggtcggc aggccggccg ctgcaagatg cgaagatgcg cgggcggcgt ccgacgccac
     4441 gatcagcccc ttttttgccg agaattcgaa cgccagcgcc attcccagcg ccaccgcctc
     4501 gccgtgcaac agccggtcgg agaacccgca gccggcttcc agcgcgtggc cgaacgtgtg
     4561 gccgaggttg agcagcgcac gctcgccggt ctcgcgctcg tcacgcgcaa caattccggc
     4621 cttggcgcgg cagcacaccg cgatggcgtg ctcgcgcgcg ggcccgccgg caaagacatc
     4681 ctgccagttt ttttccagcc aggcgaaaaa cgcggcatcg ccgagcaggc catatttagc
     4741 gacctcggca taaccggcgc gaaattcccg tttcggcaac gtgtcgagca atgccgaatc
     4801 tgcgacgacg agtaccggtt gatgaaacgc gccgacgagg ttcttgccct ggcgcgagtt
     4861 gatgccggtc ttgccgccga cggacgagtc gacctgcgcc agcagcgtcg tcggcacctg
     4921 cacgaaatcg agcccgcgcc tcgcgcacgc cgccgcaaac ccggcaagat cgccgaccac
     4981 gccgccgcca agcgcgacca caagatcgtt gcgctcaacg cgcgccgcga taatagcctc
     5041 gcacaccgtt tcgaacgttg cgtagttctt ggagctttcg ccggccggca ccacgatgcg
     5101 ggcacaatcg attccggccg cgccgagcga cgcctcggtg gcggcgagat gatgagccgc
     5161 caccgcctcg tcggtcacga tcgccacgcg tgcggccgaa cgcagatttt tgatgcgcgt
     5221 gcccaggctc gcaagcaggc cgcgaccgat gaagatgtcg taagttcttt cgcccagcgc
     5281 gaccggcacg acgatgggct cgctttgacg gagcggcgcc gtcatgacga cttctccgct
     5341 ccgagctcaa gacgcttccg gagttcgccc atgatctcgt cgatgatggt gtcgtgcggt
     5401 tcgtcacgcg actggacgat aatgtgggcc tgcgcataga gcggctcgcg caagggcaga
     5461 aggtccctga tcctttccac cagcggccgg tcattgcggc gcttggtgcg cttgagcagc
     5521 acgtcgatgt ccgccttcag ccagatcgag atgccctttt cgccgatgag ggcgcgggtc
     5581 tgtgcgtcca ttacggagcc gccgccggtc gcaagcacct gcgggccgtt gtcgagcagg
     5641 cgcgcgatca cgcgcgcctc gcctgcccgg aaataaggtt cgccatgctt ctcgaaaatt
     5701 tccggaatgg tcatgccagc cgcactttca atttcggtat cggcatcgac gaagggaatt
     5761 cccagacggc cggcaagcct gcggccgatc gacgatttgc c
//
LOCUS       DASSRL010000479         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_12615, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000479 DASSRL010000000
VERSION     DASSRL010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12615"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1537)
                     /locus_tag="VFL49_09750"
     CDS             complement(200..1537)
                     /locus_tag="VFL49_09750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7912304.1"
                     /translation="MEAGWYEATSVARPDRPRLNLDLDVDVCVIGAGLAGLTVAREVA
                     QRGWSVAVLEAAHVAWAASGRNLGFVLPGYFEDIDDIIERVGLDHAKELWALSEQGLD
                     YVRRTILDSSMPGVDLINGWLHVSKTDNTAALRGEVERMRWIGADVEFWPAERVREQL
                     RSPRYFGAVHYRQAFHIDPHNYALGLAALAEKAGARIFEATPAVSLDPAGVRKRIVTP
                     DGRVRAAHVVLAGNVHLGALMPQLAATLLPVTTFVMVTEPIGEILHEIVRYRGAVSDS
                     DRADNHYRIVGGDRLQWAGRMRAWEASPRFVGRALANDVRRTFPDLGKIEIAHLWSGT
                     FGRTVHRMPQIGEIERGVWLASGFGGHGLNTSAMGGELVARGIVDSDQTWRLFAPYEL
                     VWAGGLLGRIVAQGIYWGTRPVDRFEEEIARYRARARARRQARLARKETVAAP"
     gene            1641..2174
                     /locus_tag="VFL49_09755"
     CDS             1641..2174
                     /locus_tag="VFL49_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7912305.1"
                     /translation="MTMRRLLLFRHAKAEHSEPGMEDRARRLVERGQKDAAKVGAYMA
                     SHALIPDRVMTSPSARTQETWKFAAPAFRPAPAVAPVERLYNATPHDILAIIRGAPPA
                     AKSLLVLGHNPGLHAVALMLIASGDIEARERLREKMPTCGLVIIDFAFADWGKLHPQS
                     GRLERFVTPKSLAAAAN"
     gene            2250..2675
                     /locus_tag="VFL49_09760"
     CDS             2250..2675
                     /locus_tag="VFL49_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7912306.1"
                     /translation="MYNKILVPVDLADTDLAKPAITTAIGMARSSGGSVRLVNVLAMT
                     PVMLAEYVPPDFDVQQRQSAEEALGIIARECGLDAARVSATVRQGGIYHEVLEEAKAF
                     NADLIVMSSHRPAMRTYFLGSNAGHVVRYAKCSVLVVRN"
     gene            complement(2693..>2917)
                     /locus_tag="VFL49_09765"
     CDS             complement(2693..>2917)
                     /locus_tag="VFL49_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912307.1"
                     /translation="NRLVYWLGILVFVGAALGLAALIARALQAGATTSAAIVGAFLAL
                     YLWQAGNFFRRNRPGAYRPDALPADVMPRV"
BASE COUNT          565 a          961 c          898 g          493 t
ORIGIN      
        1 aagacgcgcg gtatagccgc gatcgaccgc gaccacatca gaagcgaagg tgaaatcaga
       61 aaccaaaaca attttcggtt tcggttccgt cgcggcggcc cctgactgga ggccgttgta
      121 aacggtgcag ccgctcaaca ggagcgcggc agccgttagc tgcaggagtg tcagaggcag
      181 tgagcgcgtc atggagatcc tagggcgcgg caacggtttc cttgcgcgcc aaccgcgcct
      241 ggcggcgggc tcgcgcacga gcacgatagc gggcgatttc ttcttcgaaa cggtcgaccg
      301 gccttgtacc ccagtaaatt ccctgggcga cgatccggcc gagcaaaccg ccggcccaga
      361 ccaattcata gggcgcaaaa agccgccagg tctgatcgct gtcgacaatg ccgcgcgcaa
      421 cgagctcgcc acccatcgcg ctggtgttga gcccgtggcc accgaagccg cttgccagcc
      481 atacgccgcg ctcaatctcg ccgatttgcg gcatgcgatg aaccgtccgc ccaaacgttc
      541 cactccataa atgagcaatc tcgatctttc cgagatccgg aaaggttcgc ctgacgtcat
      601 tggcgagcgc cctgccgacg aagcgcgggc tggcctccca ggcgcgcatg cggccagccc
      661 actgcaggcg atcgcctccg acgatgcgat agtggttgtc ggcgcgatcg gaatcgctga
      721 ccgcgccacg gtaacggacg atctcgtgca ggatttcgcc gatcggctct gtcaccatca
      781 cgaaagtggt gaccggcagc aatgttgccg cgagttgcgg catcagggcg ccgaggtgaa
      841 cattgccggc aagcacgacg tgcgctgcgc gcacgcgtcc gtcgggtgta acgatgcgct
      901 tgcgcacgcc cgccggatca agcgataccg cgggcgtcgc ctcgaaaatg cgtgcgccag
      961 ccttttcggc cagtgccgca aggccgagcg cataattgtg aggatcgata tgaaaggcct
     1021 gccgatagtg aacggcgcca aaataacgcg ggctgcgcag ttgctcccgc actcgttccg
     1081 cgggccaaaa ctcgacgtcc gcaccgatcc agcgcatgcg ctcgacttcg ccgcgcaggg
     1141 ccgccgtatt gtccgtcttc gatacgtgca gccaaccgtt gatgagatcg acgccgggca
     1201 tgctgctgtc aagaattgtg cgccggacat aatcgagccc ttgctcggat agggcccaga
     1261 gctcctttgc atggtcgagc ccgacgcgct cgatgatgtc gtcgatgtct tcgaaatagc
     1321 cgggcaggac aaatcccagg ttgcggcccg aagcggccca ggccacgtgg gctgcctcca
     1381 gtacggcaac cgaccagccg cgctgggcaa cctcgcgcgc cacagtgagg cccgcgaggc
     1441 cggccccgat gacgcaaacg tcgacatcga gatcgagatt gaggcgcgga cggtccggcc
     1501 gtgcaacgct cgtcgcctca taccagccgg cttccatgac cacgcgttct ttcctggttc
     1561 agcgacgcga tttgccgcag tgcgcggcgt cgacatggac acatgctaga ctggaccccg
     1621 ccgctgccaa catccatagc atgaccatgc gacgcctcct gctctttcgc cacgccaagg
     1681 ccgaacactc agaaccgggc atggaagacc gcgcacgccg gctggtcgag cgtggacaaa
     1741 aggatgccgc gaaggtcggc gcctatatgg cctcgcacgc cctgataccc gaccgcgtaa
     1801 tgacgtcccc ttcggcgcga acgcaggaaa cctggaagtt cgctgccccc gccttccggc
     1861 cggcgccggc tgtcgcgcct gtggaacgcc tctacaacgc gacaccgcat gacatccttg
     1921 ccatcatcag gggcgcccca cccgccgcca aaagcctcct tgtgctgggt cataatcccg
     1981 gtctgcacgc ggtcgcattg atgcttatag cgtctggcga tatcgaggcc cgcgagcgcc
     2041 tgcgggaaaa aatgcccaca tgcggactgg tcattatcga tttcgcattc gccgactggg
     2101 gaaaactgca cccgcagtcc ggccgtctgg agcgtttcgt cacccccaaa tcgctggctg
     2161 ccgccgccaa ttgaggcaat gcgcatactc aacgcccggg taaaaacgtg tcatgattgc
     2221 ggccttgcag cgaaacgagg ggcgctgtca tgtacaacaa gattctggtg ccggtggacc
     2281 ttgctgacac cgatctggcg aaacctgcca tcaccaccgc gatcgggatg gcccggagtt
     2341 ccggcggctc agtccgcctg gtgaatgtcc tggccatgac gccggtcatg ctggcggaat
     2401 acgtgccacc cgacttcgac gtccagcagc gacagtcggc cgaagaggcc ctgggcatta
     2461 tcgcgcggga gtgcggcctt gacgcggcgc gcgtttccgc caccgtgcgc cagggcggca
     2521 tctatcacga ggtgcttgag gaagcgaaag ccttcaatgc cgatctgatc gtgatgagct
     2581 cccaccgtcc cgcgatgcgc acatattttc tggggtcgaa cgccggccac gtcgtgcgct
     2641 acgccaaatg ttccgtgctg gttgtgcgca actgataaac tcgtccgcaa gcctacaccc
     2701 ggggcatgac gtcggctggc agtgcgtccg gccgataggc gcctggccga ttccggcgaa
     2761 agaagttgcc agcctgccag aggtataacg ccaggaacgc gccgacgatt gcggcgctgg
     2821 tcgttgcgcc cgcctgcagg gcgcgcgcga tcagtgcggc gagcccaagc gcggctccga
     2881 cgaaaacgag gatgcccagc cagtagacca gccggtt
//
LOCUS       DASSRL010000480         6159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_590067, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000480 DASSRL010000000
VERSION     DASSRL010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590067"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFL49_09770"
     CDS             complement(<1..513)
                     /locus_tag="VFL49_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET7912308.1"
                     /translation="MKRAAVTTFVVFFVLAPAAALAHPGHDGAGGLVQGFVHPVTGVD
                     HVLAMIAVGVLAALYGGRALWLVPMSFLVAMAAAGAIGMAGIPVQIVEAGIGLSIVVL
                     GLMIAFQIKPPALVAMVVVGFFALFHGYAHGSEMPDGAAGLSFAAGFLIATALLLSAG
                     AGLGLLLQRRT"
     gene            772..1872
                     /locus_tag="VFL49_09775"
     CDS             772..1872
                     /locus_tag="VFL49_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7912309.1"
                     /translation="MPMSIDRGWKLCAAAATLVASVVLCSCSPQTETSAPQPRPVRTV
                     TIEKREAGQAITLTGRIEAEDEVALSFRISGRVLENDMKLGDRVKAGQVVARLESQNE
                     RNTLRAAQANLVASQGQLTQARNHFERQDTLLQQGWTTRANHDQAKQALQTAESQVDA
                     AEAQVKTAYDLVSFTELKADAPGVITAVGPGAGEVVQAGQMIVRVARRDGRDAVFDVS
                     AQVMRTAPSDPRITVSLTDDPKVTTTGRVREVAPQADPVTRTFAVKVGLNDPPPAMRL
                     GATVTGRMEGDSVPVINIPATALTKFNSQPAVWVVDPSSKTVSIRNVDVLRFDPARVA
                     VSGGLTTGEVIVTAGVQALHPGQKVRLLGSEQ"
     gene            1874..4936
                     /locus_tag="VFL49_09780"
     CDS             1874..4936
                     /locus_tag="VFL49_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7912310.1"
                     /translation="MGFNLSEWALKNRSLTVYLMIIAVLAGLFAFVRLGRNEDPSFVI
                     KTMVVQAAWPGASVDDMLKQVTERLERTLQETPNLDFLRSFTRAGVTTIFVNLKDSTR
                     AKEIPDIWYHVRKSIGDMRHTLPVGVVGPGFNDEFGDTFGIIYGFTADGFTHRELRDY
                     VEDVRSKLLHVPDVSKIEILGAQDEKIFVEFSMEELASLGIDRAALVAALQAQNVVQP
                     AGTIQTGNESLSLRVSGAFRSEEDVANVNFAVGGRMLRLSDIARIRRGFADPPQPLFR
                     VNGQPAIGLAIAMRDGGDILALGRNIKAALTEATADLPIGIEPKLVADQAATVDIAIS
                     EFMTSLWQAIAIILAASFISLGIRPGFVIASAIPLTLVVVFSVMLASHIDMQRISLGA
                     LIIALALMVDDAMTTTDAMLTRLAAGDDKVEAATFAFRTYAFAMLAGTLVTIAGFVPV
                     GFAASGAGEYTFSLFAVVAMALIASWFVAVLFAPLLGIAILKPPKAAKSAEPGWVFRT
                     YRSFLTWALRAKWLTIALTLAMFVASIFAMRLIPNQFFPASDRPELLVDLTLPQNASI
                     FASETVARRIDDVLKDDPDVARWSTNVGRGAIRFYLPLSVQLPNNFFSQAVVVAKDVP
                     ARNRLQKKLEDVLANEFPSLTSRVSTLELGPPVGWPVQYRISGPEISKVRDIALRLAQ
                     VIAANPDVAHINFDWFEPARQVRIQIDQNEARLLGLSSQVLAGVLNTVISGSSITQVR
                     DDIYLVDVVIRATDEERVSLDTLRSIQVPLPNGRTVPLSQFASFTYDQEQPLVWRRDR
                     VPTLTVQADVRAGMLPATVVDALTPSVTELAKTLPRPYRIVVGGTVEDSAKAQASVLA
                     VVPAMLAIMFTVLMAQLRSFSRLFIVLSVGPLGLIGVVGALLLSGKPLGFVAILGILA
                     LLGMITKNAVILIDQIESERSQGKDVWQATVDASSTRFRPIMLTAISTVLGMIPIAPT
                     VFWGPMAFAIMGGLLVGTILTLVFLPTLYVAWFRGKENHSGAKAATAS"
     gene            4982..6019
                     /locus_tag="VFL49_09785"
     CDS             4982..6019
                     /locus_tag="VFL49_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7912311.1"
                     /translation="MAKQSRPDREPVLVDLALQGGGSHGAFTWGVLDRLLEEPWLRID
                     AISGTSAGAMNGTVLVAGFAAGGAEGAKSALSAFWERVSHSARFSPIRRSPVDVMLGR
                     WTLDTSPFYLAFDLASRVFSPYDLNPRGTNPLRDVLADSVDFSQVSSSPIRLFVTATN
                     VRTGRGRIFRNAEITPDVLLASACLPTLFQAIEIDGEPYWDGGYSGNPTITPLVRESN
                     AQDTLLVAVNPVERPGTPRSAREILDRLNEVSFNATLLKELRMIALLRQVADPGKSEG
                     AQWANMRIHLIASSVLQTLGASSKFNAEWDFLCMLRDEGRRCADEFLSAKAANIGKQS
                     SVDLDVLLEQV"
BASE COUNT         1214 a         1879 c         1829 g         1237 t
ORIGIN      
        1 agtccgccgt tgcaagagca gcccaagtcc tgcgcccgca ctcagcagca aagccgtcgc
       61 gatgagaaag cccgcggcaa atgacaggcc tgctgctcca tcaggcatct cagagccgtg
      121 cgcataaccg tggaacaatg cgaaaaatcc gacgaccacc atggccacca acgccggagg
      181 cttgatttga aaggcgatca tcagcccgag gacgacgatg gagaggccaa ttccggcctc
      241 gacgatttgc accggaattc cggccatgcc gatggcgccc gcggccgcca ttgcgaccag
      301 aaagctcatt ggcaccagcc acaaagcgcg gccaccatac agtgcagcca gcacgccgac
      361 ggcgatcatc gctaaaacat gatcgacgcc ggtgaccgga tggacgaagc cctgcaccag
      421 cccgcctgca ccatcatgcc cgggatgggc cagcgctgca gccggagcaa gaacgaaaaa
      481 aacaacgaat gtcgttacag ccgcacgttt catggacatg ctccatcgaa cctggtcatt
      541 tgacgacgcg agaggagact tagcaaccac ccgaaggagc gaaaactgct cctagtttca
      601 ctgtatcaac cgcataaagt gtgcctattg gcccgaagtc ccatatctgc ggcgtcgacg
      661 ccgctttagt ctcgagcagg ggatcagaac tgacacctca aacttccgta cattgaggtg
      721 aaatatttaa tttccctcat gatttttcga atccagcagg aagagttgga catgcctatg
      781 agcattgatc gcggatggaa actgtgcgcc gccgcggcca cattggtggc gtcggtggtc
      841 ctttgtagtt gctcgccgca aacggaaacc tccgcgcccc aaccacgtcc ggtgcgcacc
      901 gtcacgatcg aaaaacgcga agccggacag gccattacct tgaccggccg gatcgaggcc
      961 gaagacgaag ttgcgttatc gtttcgaatt tcaggcagag tgctcgaaaa cgacatgaag
     1021 cttggggatc gcgtgaaagc gggacaagtt gtggcccggc tggaatccca aaacgagcgc
     1081 aacacgttac gtgcggcgca agcaaatctc gtcgcgtcgc aggggcaact gacgcaagcg
     1141 cgtaatcatt tcgagcggca ggacacgctg cttcagcagg gctggaccac gcgcgcaaat
     1201 cacgaccagg caaaacaggc actgcaaacc gcggaatcgc aggtcgatgc cgcagaggcg
     1261 caagtgaaaa ccgcctatga cctcgtcagc tttacggagc tcaaggccga tgcccctgga
     1321 gtgatcaccg ccgttggccc tggcgcgggt gaagtcgtgc aggccggcca gatgatcgtc
     1381 cgcgtcgccc ggagggacgg acgcgacgca gtatttgacg tatcggcgca ggtcatgcgc
     1441 accgcgccga gcgatcctcg catcacagta agccttaccg atgatccgaa agtgacgacg
     1501 accggccgcg tgcgcgaggt cgcgccgcaa gcggatcccg tgacgcgaac tttcgcggtc
     1561 aaggtcggcc tgaacgatcc tccaccggcg atgcggcttg gcgcaaccgt gacggggcgc
     1621 atggagggcg attcagtgcc cgtcatcaac atcccggcaa cggcactgac gaagttcaac
     1681 agtcaaccgg ctgtctgggt cgtcgatcct tcgagcaaga ctgtttcgat ccggaatgtc
     1741 gacgtgttgc ggttcgaccc ggcaagagta gcggtctcag gcgggcttac gaccggcgaa
     1801 gttatcgtca ccgcaggcgt gcaagccctg caccctggcc agaaggttcg gctgctcggg
     1861 tcggagcaat gatatgggct tcaacctttc ggaatgggcc ctcaagaacc ggtcgctgac
     1921 cgtctacctg atgatcattg cggtgctggc ggggctgttc gccttcgtgc gcctcgggcg
     1981 caatgaagat ccttcgttcg tcatcaaaac catggtggtt caggccgcct ggcctggtgc
     2041 cagcgtcgac gacatgctca agcaggtcac ggagcggctc gagcgcacgc tgcaggaaac
     2101 gccaaacctc gatttcctcc gcagctttac gcgcgccggt gtgaccacga tctttgtcaa
     2161 tctcaaggac agcaccagag ccaaggagat tccggacatc tggtaccatg ttcgcaaaag
     2221 tatcggcgac atgcgccata ccttgccggt gggcgtcgtt gggccgggct tcaatgatga
     2281 gttcggcgac acattcggga tcatctacgg cttcaccgcg gacggtttta ctcaccggga
     2341 attgcgagac tacgtcgagg acgtgcgctc aaagctgctg cacgttcccg acgtttcgaa
     2401 aattgagatt ctcggcgccc aggatgagaa aatcttcgtc gaattctcga tggaagagct
     2461 ggccagcctt ggcatcgatc gcgcggccct ggttgcggcg ctgcaggcgc agaacgtggt
     2521 acagcccgcc ggcactattc agacaggcaa tgaatcactg tcgctccgcg tctcgggagc
     2581 attccgttcc gaggaagacg tggccaacgt caactttgcc gttggcggcc gcatgctgcg
     2641 cctgagcgat attgcaagaa tccgtcgtgg ctttgccgat cccccacaac ctttgtttcg
     2701 cgtcaacgga cagccggcga tcggcctggc catcgccatg cgcgatggcg gcgacatcct
     2761 ggctctggga agaaacatta aagcagcgct aaccgaggcg acggcggatc tgccgatcgg
     2821 catagaaccc aaactggtgg ctgatcaggc tgcgaccgtc gacattgcca tttccgaatt
     2881 catgacgtcg ctatggcagg caatcgcaat catcctggcc gcgagtttca taagcctggg
     2941 cattcgtccg ggttttgtga tcgcctcggc cattcccctc acgctggtcg tggtcttctc
     3001 ggtcatgctg gcgtcccaca tcgacatgca gcgcatctcg ctgggcgcgc tgatcatcgc
     3061 gctcgctttg atggtcgatg atgccatgac gaccaccgat gccatgctga cgcggctggc
     3121 cgcgggtgac gacaaggtag aggcggcgac gttcgcgttt cgcacttatg cctttgccat
     3181 gcttgccggc accttggtaa cgatcgcagg ctttgtgccc gtcggttttg ccgcgagcgg
     3241 cgcgggcgaa tatacgttct cgctttttgc cgtggtcgcc atggcgctga tcgcatcctg
     3301 gttcgtcgcg gttctgttcg ccccgctgct gggtatcgcc attttgaaac caccaaaggc
     3361 ggcgaagtca gcggaaccgg gctgggtctt ccgcacctac cgctcgttcc tcacctgggc
     3421 actgcgtgcc aagtggctga cgatcgccct tacgcttgca atgttcgtcg catcgatatt
     3481 cgcgatgcgc ctcattccca accagttctt tccagcatcg gaccggcctg aactgctcgt
     3541 agacctgacg ctgccgcaga atgcctcgat ctttgccagc gagaccgtgg cgcgccgtat
     3601 tgacgatgtc ctcaaggacg atccggacgt cgcgcgctgg agcaccaatg tcgggcgcgg
     3661 tgcgatccgg ttctatctgc cgctcagcgt gcagcttccg aacaattttt tcagccaggc
     3721 tgtcgtggtc gccaaggacg tgcctgcacg caaccgcctg cagaaaaagc tcgaagacgt
     3781 gctggccaat gagttcccca gcctcaccag ccgcgtctct acgctggagt tgggtccgcc
     3841 cgtcggatgg ccagtccagt accgcatcag cggcccggaa atttcgaagg tacgcgacat
     3901 cgcactgagg ctggcgcagg tgattgcggc aaatccggac gtcgcgcaca tcaatttcga
     3961 ctggttcgag ccggcccggc aggttcgtat ccagatcgac cagaacgagg cgcggttgct
     4021 tggtctgagc tcgcaggtcc tcgctggcgt ccttaatacc gtcatctccg gcagttccat
     4081 cacgcaagtt cgcgatgaca tttacctcgt cgacgtcgtc atacgcgcga ccgacgagga
     4141 acgcgtttcg ctcgacacat tgcggtcgat tcaggttcca ttgccgaacg ggcgaacggt
     4201 accgctcagc caatttgctt cgtttacgta cgaccaggag cagccattgg tctggcgccg
     4261 tgaccgtgta ccgaccttga cggtccaggc cgacgtcaga gcgggcatgc tgccagcgac
     4321 cgtcgttgat gcactgacgc cgtccgtcac tgaattggcg aagacgctgc cgcgccccta
     4381 tcggatcgtc gtgggaggaa ccgtggagga cagcgccaag gcgcaggcct cggtgctcgc
     4441 cgtcgtgccg gcgatgctgg cgatcatgtt caccgtcctg atggcgcaac tgcggagctt
     4501 ctcgcgcctt ttcatcgtcc tcagcgtcgg accgctcgga ctgatcggcg tcgtcggtgc
     4561 cctgttactg tccggcaagc cgcttggatt cgtcgccatt ctcggaattc tggcgctgct
     4621 tggcatgatc accaagaatg ccgtgatttt gatcgatcaa atcgaatctg aacgaagtca
     4681 gggcaaagac gtctggcaag cgacggtcga cgcgagcagc acgcgttttc gcccgatcat
     4741 gctgacggcg atctcgacgg ttctcggcat gattccaatc gccccgacgg tcttttgggg
     4801 accaatggcc tttgcgatca tgggaggatt gctcgtgggg accatcctga cgctcgtctt
     4861 tttgccgaca ttgtatgtcg cctggttccg cggaaaagaa aatcattcag gtgcgaaagc
     4921 cgcgaccgcg agctagcgct acaccgccgc ccggcgggac cgcattgaat gcgaggtacc
     4981 catggcaaag caatcgaggc ccgaccggga gccggtgctc gtagatctcg cgttgcaggg
     5041 tggtggatct cacggtgcct tcacgtgggg cgtgctggac cggctgctgg aagagccctg
     5101 gctgcgcatc gatgccatat cgggcacgtc cgccggagca atgaatggca cggtactggt
     5161 cgcgggattt gccgcgggcg gagctgaagg cgcgaagtcc gcgctcagcg ctttctggga
     5221 gcgtgtttcg cactcggcgc gcttcagtcc cattcggcgc agtccggtcg acgtcatgct
     5281 gggccggtgg acgctggata catccccatt ttaccttgct ttcgacttgg ccagccgcgt
     5341 cttctcgccg tacgatttga acccgcgcgg caccaatccg ctgcgggacg tcctcgccga
     5401 ttcagtcgat ttcagtcagg tctcaagctc cccgatccgg ttgtttgtga ccgcgaccaa
     5461 cgtccgaacc ggccgcgggc gcatctttcg caacgccgag atcacgcccg acgttctcct
     5521 ggcttctgcc tgtctgccga cgctgttcca ggccatcgaa atcgacggag agccctattg
     5581 ggatggcggt tattccggca atccaacgat cacccctctc gtccgcgagt ccaacgcgca
     5641 ggacacgctt cttgtcgcgg tcaatcccgt cgaacgtccg ggtactcccc gatctgcccg
     5701 cgagattctc gaccggttaa atgaggtctc gttcaacgcg acgctcctca aggagctgcg
     5761 catgatcgca ttgttgcgcc aggtagccga tccaggaaaa agcgagggag cgcagtgggc
     5821 gaacatgcgt attcacctga tcgcaagctc ggtgcttcag acgcttgggg catcttccaa
     5881 attcaatgcc gaatgggact ttctgtgcat gttgcgcgac gaaggccggc gttgcgcaga
     5941 tgaattccta agcgccaaag cggcaaatat cggtaagcaa tcgtccgtcg acctggatgt
     6001 gctcctggaa caagtctgat gcagtgggtc gtcgtcatat tctaggttcg acacgcgggt
     6061 cgctttagca atcaccgccg atttggagag cgttatgccg gcgacgctac agtcgatagt
     6121 gttgcaaatc ctttcacttc tgctcggctt cgcgctcat
//
LOCUS       DASSRL010000481         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1447684, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000481 DASSRL010000000
VERSION     DASSRL010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447684"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VFL49_09790"
     CDS             <1..1321
                     /locus_tag="VFL49_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HET7912312.1"
                     /translation="TIAIQVWNGEVLAAGDLFRVDALSALMVVIIIFLGAIASVYAVG
                     YIRADLDDPHLSRVRLFFSLFHLFLFTMLVAVTTDNLGVMWVAIEGTTLATVFLVNLR
                     RSRTSLEAAYKYLILSSVGIALAFIGTALVYYAGAASAGEIAVNWSTLRAAAASLNRN
                     VIRVAFVFIIIGYGTKAGLAPMHTWLPDAHSEAPAPISALMSGVLLNVGLYAILRFKA
                     VVDVALGPEFAAHLLIGIGLLSLLVAAAFLVRQRNYKRMLAYSSVEHIGIICLGLGFG
                     GYWGTFGALFHVVNHALSKSLLFILSGNILLKYHSAEIDDVRGLLHAAPWTGALFVAG
                     ILALVGLPPFALFVSEFIIFRAGVGSYPWAAAVGVLLLVIIFGGMLASVNRMLYGDPP
                     AALTRGDPLRLWLAPLALNFALLIILGLTLPPPFEAALKQALQVLGV"
     gene            1326..>2768
                     /locus_tag="VFL49_09795"
     CDS             1326..>2768
                     /locus_tag="VFL49_09795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET7912313.1"
                     /translation="MADLTELKQLLLAANLDLVGFELRSSGELRVIVPRQKLTQFAAY
                     LRALNLARPELIVAEDTRSEGSGFTLRYIFALERAAELVIASVRVPADDRTFPSLATR
                     WYLASRFEREIHDLFGLVPIGNPDLRRLPLHQFWPADCHPLLKDAQLRREFSDDGEPY
                     PFRRVEGEGVYEITVGPVHAGIIEPGHFRFSAEGETIIHLESRMYFVHKGIEKLFESA
                     ALDQGVRLAERISGDSSVAHALAYCQAVEALAGVTIPARAAYLRVVLLELERLYNHIA
                     DVGAICTDTGFALANVHALRLREDLLRLNQRLLGHRLLRGALLPGGIARDLTEEQIVD
                     VRDSLARVIADFDRIVEIASHNGLVLDRLHGTGCLNTQTAREMQVVGVAARASGIDED
                     ARRDHPFAAYAALRPRVVVYREGDVWARLMVRVEEAREAVRLIDAALEALPGGDLTAP
                     LDSLPEGEGAFGLVEGWRGPIWHWVVGGKNN"
BASE COUNT          464 a          809 c          837 g          658 t
ORIGIN      
        1 gacaatcgcc attcaggtgt ggaatggcga agtgctcgcc gcgggcgatc tgtttcgcgt
       61 cgacgcattg tcggcgctca tggttgtcat cattattttc ctcggcgcga tcgcttcggt
      121 ttacgccgtt ggctacattc gcgccgactt ggacgacccg catctttcac gagtccgatt
      181 atttttctcg ctctttcacc tttttctttt caccatgttg gtcgcggtga cgacggacaa
      241 ccttggcgtc atgtgggtcg cgatcgaagg cacgacgctt gcgacggtct tcctcgtcaa
      301 tttgcgccgg agccgcacgt cactcgaagc cgcttataaa tatttgattt tgtcgtccgt
      361 cggtatcgcg ctggcgttca tcggcaccgc gttggtgtac tacgccggcg ccgcaagcgc
      421 aggcgaaatc gcggtcaatt ggagcacgct gcgtgccgcg gcggcatcgc tcaacaggaa
      481 cgtgatccgg gtggcattcg tgttcatcat catcggctat ggaaccaagg cagggctggc
      541 gccgatgcat acgtggctgc ccgacgcgca cagtgaagcg cctgcgccga tcagcgcgct
      601 tatgtccggc gtattactca atgttggcct gtacgcgatc ctgcgtttca aagccgttgt
      661 cgatgtcgcg ttggggccgg aatttgcggc gcatttgctc attggcatcg gattgttgtc
      721 gctgctggtc gcggcggctt tccttgttag gcaacgcaat tacaaaagaa tgcttgccta
      781 ctcgagcgtc gaacatatcg gcatcatttg tctcggtctt gggttcggcg gttattgggg
      841 cactttcggc gcgctgtttc atgtggtcaa tcatgcgcta tcgaagtcgc tgctcttcat
      901 actttcaggc aatatcctgc tcaaatacca cagcgcggag atcgacgacg tgcgcgggct
      961 gttgcacgcg gcgccgtgga ccggcgcgct ttttgtcgcc ggcatcctcg cgttggttgg
     1021 cttgccaccg tttgcgctct tcgttagcga attcatcatt tttcgcgccg gcgtgggcag
     1081 ctatccatgg gcggcggccg tcggcgtgct tttgctggtc atcattttcg gcggcatgtt
     1141 ggcaagcgtc aatcgaatgt tatacggcga tccgcccgcc gcgctcacgc gcggcgatcc
     1201 cctgcgccta tggctcgcgc cgctcgctct taattttgcg ctcttgatca tactcggact
     1261 aacgctgccg cctccgttcg aggccgccct caaacaagcg ttgcaagtat tgggagtata
     1321 gatagatggc ggatctgact gagctgaagc aactgctcct tgcagccaac ctggatctgg
     1381 tcggcttcga actccgcagc tcgggcgaac tgcgcgtgat cgtgccgcgg cagaagctta
     1441 cgcagttcgc cgcttacttg cgggcgctga accttgctcg tccggagctc atcgtagccg
     1501 aggacacgcg gtcggagggc agcggtttca ctctgcgcta tatttttgcg ctggagcgcg
     1561 cggccgagtt ggtcatcgct tcagtcagag taccggcaga cgatcggacc tttccatccc
     1621 tcgccacccg ctggtatctt gccagccgct tcgagcgcga gatccacgac ttgttcgggc
     1681 tcgtgccgat cggcaatccc gacttacggc gattgccgct gcatcaattt tggccggctg
     1741 attgccatcc tttgttgaaa gacgcgcaac tgcgtcgaga gttttcagac gacggtgaac
     1801 cctatccgtt tcgccgcgtc gaaggcgaag gcgtttatga aatcaccgtc ggccccgtcc
     1861 atgccggcat catcgagccc ggacattttc gcttcagcgc cgaaggtgaa acgatcattc
     1921 atctcgaatc gcgcatgtat ttcgtgcaca aaggcatcga gaagcttttc gaatcagccg
     1981 cgctcgacca aggagtcagg ctggcggagc ggatatccgg cgattcgagc gtggcgcacg
     2041 ccctcgctta ttgccaagct gttgaagcgc ttgccggcgt gacaattcca gcgcgcgccg
     2101 cttatctgcg cgtcgtgctg ctcgagctcg aacgactcta caatcatatc gccgacgtcg
     2161 gcgccatttg cacggacacg gggtttgcgc tcgccaacgt gcacgcgctg cgcctgcgcg
     2221 aagatctttt gcgcttgaat cagcgtttgc tcgggcaccg cctcttgcgg ggtgccctgc
     2281 taccgggcgg gatagcgcga gatttgacag aagagcagat tgtcgacgtc cgtgactcac
     2341 tggcccgtgt cattgccgat ttcgatcgga ttgtcgagat cgctagccat aacggtttag
     2401 tgctcgatcg tttacacggt actggctgtt tgaacacaca gactgcgcgc gagatgcaag
     2461 tggtcggtgt tgccgcgcgc gccagcggca tcgatgagga tgcgcgccgc gaccacccct
     2521 tcgcagccta tgccgcgttg cgaccgcgtg tcgtcgttta tcgcgaagga gacgtctggg
     2581 cacgtttgat ggtgcgcgtg gaggaagcgc gcgaggcagt acgattgatc gatgctgcgc
     2641 tggaggcact gccagggggc gatctcaccg ccccactcga ctctctgcca gaaggcgaag
     2701 gcgctttcgg cctggtggaa ggttggcgcg gtccgatctg gcactgggtg gtcgggggga
     2761 aaaacaat
//
LOCUS       DASSRL010000482         7246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_607524, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000482 DASSRL010000000
VERSION     DASSRL010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607524"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VFL49_09800"
     CDS             <1..1777
                     /locus_tag="VFL49_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HET7912314.1"
                     /translation="GSLDNVSLVILLGSMLVLAGILSSLIAMRFGAPLLLVFLLVGVL
                     AGENGPGGIKFDDVGVAYTVGSIALALILFDGGLRTRFATFRNVLAPAVTLATVGVLI
                     TAILTAPVAKLILGLNWIEALLIGAVVASTDAAAVFFLINARGLRLRPRVRAVLEVES
                     GTNDPFAIFLALLLVEILLVGNQSWTHALMTFLLDAILGCITGFLGGRIIAFVINRLT
                     LAQGLHAPFVAVSALVIFAFANILHSSGFLAVYLAGLVVGNTQTRARSSVIVFLDAIT
                     WLAQIVMFVLLGLLAWPNRLAYSFFGAIAVALVLMLIARPIAVFLCLAPFKFQWREMT
                     FVSWVGLRGAVGIFLASIPMLVGLPGATLFFDVAFVVVLLSLLVQGWTVALAARKLDM
                     SFARADPLPRRVELDLPGQLTREIVGYPVPESSPFLRRGLIPNWARPTLVIRNEEILT
                     PAEAHPVREGDYVYLLAPPEKAQALDRFFVRLPPPARPDPRLLGDFYVPGTATLGALA
                     DIYGLQVAADHTEVTLADYFSEQLRRRARTGDIVELGPIALLAHKVESGRVTTVGLRL
                     AEPEDGAGLGSRFESLRRWIRGYFA"
     gene            complement(1774..2319)
                     /locus_tag="VFL49_09805"
     CDS             complement(1774..2319)
                     /locus_tag="VFL49_09805"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HET7912315.1"
                     /translation="MPTTDKRANLRMDLLLVALLIALNVAARLLPHAPGFLPVAASGL
                     FAARVLSIPMLALLVPVLGMILSDIALPGEDWRIQAVGFAAIAIPAIAGMATRRWRGV
                     VPVVATMIPCSILFFLLSNGAVWAFSGMYSLTWQGLVQCYVAALPFLDKTVLGDLFWT
                     AVLFGGAWLVQHVPALSRRAF"
     gene            complement(2332..3189)
                     /locus_tag="VFL49_09810"
     CDS             complement(2332..3189)
                     /locus_tag="VFL49_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET7912316.1"
                     /translation="MFPPERIICLTEETVETLYLLGEDRRIVGVSGYAVRPPQVRREK
                     PRVSAFISADVPKILDLKPDLVLTFSDLQADIVAELIRGNVAVHAFNQRDIAGIFDMI
                     RTLGALVGATTKAEALVSSLARHVDDVHERSLHLPRRPRVYFEEWDEPMISGIAWVSE
                     LIEAAGGIDVFPQLASRKNAKDRIVSPRDVIVASPDVIVGSWCGKKFVPGKVTARPGF
                     AEVPAVTTGWLREIKSTLILQPGPAALTDGLDAMASIIKNWAEHRSSDRPSDDSNHGR
                     RRAKGFPDN"
     gene            complement(3193..3879)
                     /gene="modC"
                     /locus_tag="VFL49_09815"
     CDS             complement(3193..3879)
                     /gene="modC"
                     /locus_tag="VFL49_09815"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HET7912317.1"
                     /translation="MLAFQAEKQLGEFSVNVSFTSDTGATALFGPSGAGKTSVINMIA
                     GLLSPERGHIVLDGEVLFDSARRINVPAWRRRIGYVFQEGRLFPHFSVKRNLDYGRWI
                     SGHTSDPKEFGHVIQLLDIGHLLDRRPGKLSGGERQRVAVGRALLMQPRLLLLDEPLA
                     SLDAARKHDILPYLERLRDEARVPLIYVSHNAPEIKRLATRVVRLESGKVAETGGVEL
                     LDGVRADMLA"
     gene            complement(3882..4577)
                     /gene="modB"
                     /locus_tag="VFL49_09820"
     CDS             complement(3882..4577)
                     /gene="modB"
                     /locus_tag="VFL49_09820"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET7912318.1"
                     /translation="MLELSPQDWTAIELSLRVAVVSTLIAMPFGIAIATLLARGSFWG
                     KSMLDALVHLPLVLPPVVTGYLLLITFGRKAPVGAFLADHLGIVFSFRWTGAALACGV
                     MAFPLMVRAIRLSIEAIDQRLEHAAATLGANPIWTFATVTLPLALPGIIAGMMLAFAR
                     ALGEFGATITFVSNIPGETQTISAAIYTLTQVPGGDAAAFNLVIVAIIISFAALLASE
                     WFARRAAARLHGS"
     gene            complement(4592..5377)
                     /gene="modA"
                     /locus_tag="VFL49_09825"
     CDS             complement(4592..5377)
                     /gene="modA"
                     /locus_tag="VFL49_09825"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7912319.1"
                     /translation="MFVRFFAFVSFLAALAGAPQPLIAQEQITVFAAASLKNALDDTN
                     AAFTKATGVKVIVSYAASSALAKQIEQGAPADIFISADLQWMDYLGERKLIKPDTRFN
                     LLGNKLVLIAPADSRIGNVTIGPGFDIAKLAGSGRIAVADVNAVPAGRYARAALEKLG
                     AWAKAEPKLAQAENVRATLAFVARDETPIGIVYETDAKVEPKVKVVGAFPGHSYPPVT
                     YPVAATAITTKQSVPQYLHFLRTPAAKAIFEKYGFSFLIRPVS"
     gene            5551..7173
                     /locus_tag="VFL49_09830"
     CDS             5551..7173
                     /locus_tag="VFL49_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7912320.1"
                     /translation="MTSIEGEFDYIIVGAGSAGCVLANRLSANPHNRVLLLEAGGKDN
                     WIWFHIPVGYLFAIGNPRADWLLKTEAEPGLNGRSLNYPRGKVVGGSSAINGMIYMLG
                     QSADYDNWRQLGLPGWSWEDVRPYFRKHVDHFLKSEHHAAGGEWRVEFPRLNWEILDA
                     FRQAANQYGIPTVDDFNTGDNEGICYFHVNQKRGRRWSAARGFLRPVLHRQNLRLETG
                     CLVEGLELTGTRASGVRWRQNGDRRSAKARGEVILAAGAIGSPHIMMLSGIGPAAQLA
                     EFGIPVALDKPGVGANLHDHLQLRMIYKVSGVKTLNETYASLFNRLGMGLNYAFRRRG
                     PMTMAPSQLGAFTRSDSTQDRANIQYHVQPLSLDKFGEPLHPFPAFTASVTNIRPTSR
                     GALMLKSADPATPPAISPNFLSTPEDQHVAADSIRVTRGIVRQPALQKFSPVEYLPGD
                     SVRDDDETGLIKAAGDIGTTIFHPVGTARMGRDDDTRAVVDARLRVTGIDRLRVVDAS
                     VMPSITSGNTNSPTMMIAEKGSAMILEDQKRL"
BASE COUNT         1377 a         2196 c         2272 g         1401 t
ORIGIN      
        1 tggttcgctc gacaatgtaa gcctcgtgat cctgcttggc agcatgctgg ttctcgccgg
       61 catcctgtcg agcctgatcg cgatgcgctt cggggcaccg ctgctgctgg tcttccttct
      121 ggtgggcgtt ctggcggggg agaacgggcc cggcggcatc aagttcgacg acgtcggcgt
      181 cgcctatacg gtcggctcga ttgcgctggc gctcatcctc ttcgacggtg gcttgcgcac
      241 gcgctttgcg accttccgca atgttctggc gccggccgtg actcttgcaa cagtcggcgt
      301 gttgattacc gcaattctga cggcgccggt cgcgaaatta atccttgggc tcaactggat
      361 cgaggccctg ctgatcggcg ccgtcgtggc gtcgaccgac gccgccgccg tgtttttcct
      421 gatcaatgcc cgtggcctgc gtctgcgccc gcgggtccgg gccgtgctcg aagtggagtc
      481 cggcacgaac gatccgttcg ctatttttct cgctttgctg ctggtcgaaa ttctgctggt
      541 cggcaaccag agctggacgc acgcgctgat gacgttcctg cttgacgcca tcctcgggtg
      601 catcacaggc tttttgggcg gacgaattat cgcgtttgtg atcaatcgcc tgacgcttgc
      661 gcagggcttg cacgcgccct tcgtcgctgt cagcgcactg gtcatttttg cctttgcaaa
      721 tatcctgcat tcgtccggct tcctcgccgt ttatcttgcg ggactggtcg tcggcaacac
      781 gcagacgcgc gctcgcagtt ccgtcatcgt ctttctcgat gccatcacct ggctggcaca
      841 gatcgtgatg tttgttctgc tcggcctgct ggcctggccg aaccgtctcg cctacagttt
      901 tttcggcgcc attgcggtgg ctctcgtcct catgctgatt gccaggccca tcgccgtgtt
      961 cctgtgcctc gcgccgttca aatttcaatg gcgtgagatg acgtttgttt cctgggtcgg
     1021 cttgcgcggt gccgtgggta tctttctggc gtcgatcccg atgctggtgg gactgccggg
     1081 agccacgctt ttttttgacg tggcctttgt cgtggtcttg ttgtcgctgc tggttcaggg
     1141 ctggacggtg gcgctcgccg cgcgcaagct cgacatgtcg tttgcgcgtg ccgacccgtt
     1201 gccgagacgt gtcgaactcg acctgccggg gcaactgacc cgcgagatcg tcggttatcc
     1261 ggtgcccgaa agcagcccgt tcctgcggcg cgggctcatt ccgaactggg cgcggccgac
     1321 gctggtcatt cgcaacgagg aaattttaac cccggcggaa gcgcatccgg tgcgcgaggg
     1381 cgactatgtt tatctgctcg ctccgccgga aaaggcgcag gcactcgatc gctttttcgt
     1441 caggttgccg ccgcccgctc ggccggatcc gcggctgctc ggcgattttt acgtaccagg
     1501 cacggcaacg ctcggcgctc tggcagacat ctacggtttg caggtcgccg ccgatcacac
     1561 cgaggtgacg ctcgcggact atttcagcga gcagctccgt cggcgcgcca ggacgggcga
     1621 cattgtcgag ctaggcccga ttgcgctgct ggcgcataag gtcgagagcg gccgcgtaac
     1681 gacggtcggt ttgagactgg ctgagcccga agacggtgcg ggacttggca gccgtttcga
     1741 atcgctgcgc cggtggattc ggggctattt cgcctagaac gcgcgccggg aaagcgccgg
     1801 gacgtgctgg accagccagg caccgccgaa caggacggcc gtccagaaca ggtcgccgag
     1861 caccgtcttg tcgaggaacg gcagcgccgc gacgtaacat tgcacgaggc cctgccacgt
     1921 gagtgaatac atgccgctga aggcccacac ggcgccgttt gagagcagga agaaaagaat
     1981 cgagcacggg atcatggtgg ccaccaccgg aactacgccg cgccagcggc gcgtcgccat
     2041 acccgctatg gccgggatgg cgatcgccgc aaagccgacc gcctgaatgc gccaatcctc
     2101 gcccggcaaa gcaatgtcgc tcaaaatcat gcccaggacg ggcacgagaa gcgcgagcat
     2161 cgggatactc aggacgcgcg cggcgaagag cccgctcgct gccaccggca ggaaaccggg
     2221 cgcatgcgga agcagccgtg cggccacgtt caaggcgatc aacaaggcta ccagcagcaa
     2281 gtccatccgg aggtttgcgc gcttgtcggt cgtcggcatt ggctcgattc cttaattgtc
     2341 ggggaaacct ttagcacgcc tgcggccgtg attgctatcg tcagatggcc ggtccgacga
     2401 acggtgctca gcccagttct tgatgatcga ggccatggca tcgagcccat cagtcaatgc
     2461 cgcagggcct ggctgcagaa tcagggttga tttgatttcg cgcagccaac cggtggtgac
     2521 ggcgggaact tcggcaaaac ccggccgggc tgtgactttt cccggcacaa atttcttgcc
     2581 gcaccaggat ccaacgatga cgtccggact cgcgacgatg acatcgcgag gtgacacgat
     2641 ccgatccttg gcgtttttgc gcgaggcgag ctgcggaaaa acgtcaatgc cgcccgcggc
     2701 ttcgatcagc tcggacaccc aggcgatgcc ggaaatcatc ggctcatccc actcctcgaa
     2761 atacacgcgg ggacgtcgtg gcaggtgcaa tgaccgctcg tgaacgtcgt cgacgtgacg
     2821 cgcgagcgag ctgaccaggg cttcggcttt cgttgttgcg ccaaccagcg cgccaagggt
     2881 gcggatcatg tcgaaaatgc cggcgatgtc gcgctggttg aacgcgtgca cggcgacatt
     2941 tcctcgaatc agctcggcaa cgatgtcggc ctgcaaatcg gaaaacgtca gaaccagatc
     3001 cggcttgaga tcgagaatct tcggtacgtc ggccgaaata aaagcggaga cgcgcggctt
     3061 ctcgcgccgc acctgcggcg gccgcacggc gtagccggac accccgacaa tgcggcgatc
     3121 ttcaccaagc agataaaggg tctcgaccgt ttcttcggtc aggcagatga ttcgttccgg
     3181 aggaaacatt cctcaggcaa gcatatcggc gcggacgccg tcgagtagct cgacgcctcc
     3241 ggtttcagcg accttaccgg actcgaggcg cacgacgcgg gtggcaaggc gctttatttc
     3301 aggcgcattg tggctgacat agatcaacgg cacgcgtgcc tcatcgcgca ggcgctcgag
     3361 gtaggggagg atgtcgtgct tgcgtgccgc gtccagcgaa gccagcggct cgtccagcaa
     3421 cagcaggcgc ggctgcataa gaagtgcgcg gccgacggcc acgcgctgac gctcgccgcc
     3481 cgacagcttt cccggccgcc ggtcaagcag atggccgatg tcgagcaatt gaatcacgtg
     3541 gccaaattcc ttcggatcgc tcgtgtggcc gctgatccag cggccatagt ccagatttcg
     3601 ctttaccgag aaatgcggaa acaaccgccc ttcctgaaag acatagccga tgcggcgccg
     3661 ccaggccggc acgttgatac gtcttgcgct gtcgaagagg acttcgccgt caagaacgat
     3721 gtgcccgcgt tcagggctca gcagcccggc gatcatattg atgacgctgg tcttgccggc
     3781 gcccgaggga ccaaacaaag cggtcgctcc cgtgtcgctg gtgaatgaga cattcaccga
     3841 aaattcgccg agctgctttt ccgcctgaaa ggcaagcatc gtcagctccc gtgcagtctc
     3901 gcggcagcac gccgcgcgaa ccactccgac gcaagtaacg ccgcaaatga gataataatc
     3961 gccacgatga cgagattgaa cgcggcggca tcgcctccgg gcacctgcgt gagcgtgtag
     4021 atcgccgcag agatcgtctg cgtctcgccg ggaatattgg agacgaaggt aatggttgct
     4081 ccgaactcgc cgagcgcgcg cgcaaaggcg agcatcatgc ccgcgatgat gccgggtagg
     4141 gccaacggca atgtcacggt cgcgaaggtc cagatgggat tggcgccgag cgtcgcggcg
     4201 gcatgctcga gccgctggtc gatggcttca atcgacagcc gtatagcgcg gaccatcaac
     4261 ggaaaagcca tcacgccgca ggcaagcgcg gcgccggtcc agcggaatga aaagacaatt
     4321 ccgagatgat ccgcaaggaa ggcgccaacc ggcgccttgc gaccgaaggt gatgagcagc
     4381 agataacccg tgaccactgg cgggagcact agcggcaaat gaacgagagc gtcgagcatc
     4441 gatttgcccc agaatgagcc gcgcgcgagc aatgtggcga ttgcgatgcc aaacggcatc
     4501 gcaatcagcg tcgacacgac cgcaacccgc agcgagagct cgattgcagt ccagtcctgg
     4561 ggcgacaatt cgagcacgcg acgaataccg cttatgacac cggccttatc aggaaactga
     4621 agccgtactt ctcaaaaatc gccttggcgg ccggagtccg cagaaaatgg agatactgcg
     4681 gcacgctctg cttggtggtg atggcggttg ccgcgacggg gtatgtcacc gggggataag
     4741 agtggccagg aaatgcacct acgactttca cctttggctc gaccttggca tcggtttcgt
     4801 agacgattcc tatcggtgtc tcgtcgcgcg cgacgaacgc gagcgtcgcc cggacatttt
     4861 ccgcctgcgc gagtttcggc tcggctttcg cccaggcgcc cagcttttca agcgcggccc
     4921 tggcgtagcg tccggcgggc acagcattga cgtcggcgac cgcgatgcgg cccgaacctg
     4981 cgagcttcgc gatgtcgaaa cccgggccga tggtcacatt gccgatcctg gaatccgcgg
     5041 gtgcaatcag taccagcttg tttccgagca ggttgaagcg cgtatccggc ttgatgagct
     5101 tccgctctcc gagataatcc atccactgaa gatccgccga aataaaaatg tcggccggtg
     5161 cgccctgttc gatctgtttg gcgagcgccg aacttgcggc gtagctcacg atcaccttga
     5221 cgcccgtcgc tttcgtaaag gcggcatttg tgtcatcgag cgcgttcttc aatgatgccg
     5281 ctgcgaagac ggtgatctgc tcctgggcga tcaacggctg cggcgcaccg gcgagagcgg
     5341 ccagaaagct gacaaaggcg aaaaatctca cgaacatggc ggttctcctc ggcgcaaaaa
     5401 gcgccggata cgacctcaaa ggccagattg aattcggaac cgccgttccg gtctaaggcg
     5461 gcgcacgcac ggtgcgccac caacccagcc tagcaagtcc cgccgagggc ggtaaagtcc
     5521 gcgttggcgt gcaggggagc gagtgcggac gtgacatcga tcgaaggtga atttgactac
     5581 attatcgtgg gcgccggctc ggccggttgc gtcctggcca atcggctatc cgcgaatccg
     5641 cacaatcgcg tgctgttgct ggaggcgggc ggcaaggaca actggatctg gtttcacatt
     5701 ccggtgggtt acctgttcgc gatcggaaat ccgcgtgccg actggcttct caagaccgag
     5761 gccgaaccgg gcctgaatgg gcgcagcctg aattatccgc gcggcaaggt cgtgggcggc
     5821 tcttcggcca tcaacggcat gatctacatg ctcggccagt ctgccgacta tgataactgg
     5881 cgccagctcg gcctgcccgg ctggtcatgg gaggatgtgc ggccttactt ccgcaagcac
     5941 gtcgatcact tcctgaaaag cgaacatcac gccgccggcg gagaatggcg ggtcgaattt
     6001 ccccgcctga actgggaaat tctcgacgcc ttccggcaag cggcgaatca gtacggcatc
     6061 cccaccgtcg acgacttcaa caccggcgac aatgagggca tttgctactt ccacgtcaac
     6121 caaaagcggg gacgccgctg gtcggcggca cgtgggttcc tccggccggt tttgcatcgg
     6181 caaaatctcc ggcttgagac cggttgcctt gtcgaaggac ttgaattgac cggaacgcgc
     6241 gccagtggcg tgcgctggcg gcagaacggc gaccggcgct cggcgaaagc cagaggcgaa
     6301 gtgatcctgg ccgcgggtgc tattgggtct ccgcacatta tgatgctttc cggaatcggt
     6361 ccggccgcgc agctggcgga attcggcatt ccggttgcgc tcgacaagcc gggtgtcggc
     6421 gccaatctgc acgaccattt gcagctgcgt atgatctaca aggtgtccgg tgtgaagacg
     6481 ctgaatgaaa catacgcctc gctgtttaat cgtcttggga tgggattgaa ctacgcgttt
     6541 cgccggcgcg ggccgatgac gatggcgccg tcgcagctcg gcgcttttac caggtccgat
     6601 tcgacgcagg atcgcgccaa tattcaatat catgtgcagc ccctgtcgct cgacaaattc
     6661 ggcgagccgc tgcatccgtt tcccgccttt accgcgagcg tgaccaatat ccggccgacc
     6721 agccgcggcg cactcatgct caagtcggcc gacccggcaa caccaccggc gatcagtccg
     6781 aacttccttt cgacgccgga agaccagcac gtcgcggcgg attcgatccg ggtcacccgt
     6841 gggatcgtgc ggcagccggc gctgcagaaa ttctcgccag tcgagtatct ccccggcgat
     6901 tcggtgcggg atgacgacga gaccggcctg ataaaggcgg caggcgatat cggaaccacg
     6961 attttccatc ccgtaggcac tgcccggatg ggccgtgacg atgacacgcg ggcggttgtc
     7021 gatgcgcggc ttcgcgttac gggcatagac aggctgcggg ttgttgatgc ctcagttatg
     7081 ccgtcgatca cgtccggaaa caccaattcg ccgacaatga tgattgcgga aaaaggctcg
     7141 gcaatgattt tggaggatca aaaacgtcta taatcatcac gggataaatg gaggggtgtc
     7201 atgaatctca tggacagaat caaggggatc ctgctgcagc ccaaat
//
LOCUS       DASSRL010000483         5003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_205116, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000483 DASSRL010000000
VERSION     DASSRL010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205116"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1925
                     /locus_tag="VFL49_09835"
     CDS             <1..1925
                     /locus_tag="VFL49_09835"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HET7912321.1"
                     /translation="SPDGLTPAEQLSRIGEAVSQLAHDQQQRWIELRPELAAAGVVLV
                     DGTDIKKPERNWLEEYFLTHIFPLLTPLAIDPAHPFPFIPNLAFSIALQLARTKDGKA
                     MNALIRMPQKIERFISLPGSGDQVIRLITLEAATALFIGRLFPGYTVKGHGAFRAIRD
                     SEVEIEEEAEDLVRLFETALKRRRRGSVIRLELNASMPGELCNFVQRALAVADDEVFK
                     VDGVLALNDLVQLTKLDRPDLEFVPYNPRYPERIRDHGGDCFAAIRQKDLIVHHPYES
                     FDVVVQFLQQAARDPNVVAIKQTLYRTSAESPIVKQLVEAAEAGKSVTALVELKARFD
                     EEANIRWARDLERAGAQVVYGFIELKTHAKLSLVVRREAGELATYVHVGTGNYHPVTA
                     RIYTDLSFFTADAVIGRDVARIFNYITGYAEPAELERMAISPINLRKRILDHIAQEIA
                     HAKAGRPASIWMKINALVDPGIIDALYDASAAGVSIDLVVRGICCLRPGVSGLSENIR
                     VKSIVGRFLEHGRIYCFGAGRALPHPKAAVYISSADMMPRNLDRRVEVLCPILNATVH
                     EQVLGQIMVANFKDNEQSWQLLPDGSSTRIKAAPGEELFNAHKYFMTNPSLSGRGKSL
                     KESSPRSLIRRLIEGA"
     gene            1939..3438
                     /gene="ppx"
                     /locus_tag="VFL49_09840"
     CDS             1939..3438
                     /gene="ppx"
                     /locus_tag="VFL49_09840"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HET7912322.1"
                     /translation="MVPKGRLDHGPPIAVIDIGSNSIRLVVYEGLTRAPTPIFNEKVL
                     AGLGRQVQSTGLLPADAIDTALGALRRFRALCNILHVRRIWAIATAACRDARNGRAFI
                     AEAERICGTRISILSGKREAQLTALGVVSGIHNPDGIVGDLGGGSLELVDVHGTRVRG
                     GVTLPLGGLALQDITGKSLKKAERFVEEAFEDLDMLHDGEDRTFYAVGGTWRALARLH
                     MWQTGYPFHVMHNYRISAREAQEFSRLVHHVDPETLSKIEVVNAARRPLLAYAALVME
                     NLVRSIKPKQVIISVLGVREGLLYASLGARERVRDPLISAAANLNVLRSRSPRHGEEL
                     IAWTDRFIASSGLEETADEQRLRHAACLLGDIGWRAHPDYRGEQSMNIIANGAFVAID
                     HPGRAYLALSVYFRHAGLNEEALSPRLRELATTRMLDRARVVGAAMRVAYILTAGQAG
                     VLPKTPMQVKRGKLILKLAGGYGELASDRLASRLRQLGRLVGRESAIET"
     gene            complement(3464..4612)
                     /gene="rnd"
                     /locus_tag="VFL49_09845"
     CDS             complement(3464..4612)
                     /gene="rnd"
                     /locus_tag="VFL49_09845"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HET7912323.1"
                     /translation="MDLITTTDQLAAACARAAKHPYVTVDTEFLRETTYYPLLCVAQM
                     ASPDEAVVIDALAVGIDLSPFFELMANETITKVFHAARQDIEIVWNLAQKIPHPIVDT
                     QVAAMVLGYGDSISYDQLVQRITGDTLDKSHRFTDWTRRPLSDAQIAYALSDVTHLRA
                     VYLKLAEDLEKRGRSSWVEAEMGVLTSPDTYRADPQRAWERLKSRVRKPKELAILIEV
                     AAWREREAQVRDVPRGRVLKDDVIGDIAIQAPTTPERLGHLRSLPKGFERSRWGEQII
                     EAVKRGLERDQKTLPRLERFRPAVNGAATVELLKVLLRMTAERHGVAAKVIATVDDLD
                     RIAADDEADVPAMKGWRRELFGEKALALKHGRLALAFDKGKVVTVEKS"
     gene            complement(4660..>5003)
                     /locus_tag="VFL49_09850"
     CDS             complement(4660..>5003)
                     /locus_tag="VFL49_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7912324.1"
                     /translation="GGFLYTGNELATAIRHGIPLVAVIFVDGAFGNVRRIQQEEFGNR
                     LIASDLANPDFVKYAESFGAAGRRARGPDELRVALRESIARREPTLIEVPVGAMPSPW
                     EFIMMPRVRGT"
BASE COUNT          951 a         1514 c         1518 g         1020 t
ORIGIN      
        1 agagtccgga tgggctgaca ccggcagaac agctttcacg catcggcgag gcggtatccc
       61 agctcgcaca tgaccagcag cagcgctgga tcgaattgcg gcccgaactt gcggctgcgg
      121 gcgtggtgct tgtcgacggt accgatatca aaaagccgga gcggaattgg ctggaggagt
      181 actttctcac ccatattttc ccgctgctga cgccactcgc aatcgatcct gcgcacccat
      241 ttccgttcat tccgaacctg gccttttcca tcgcgctgca gcttgcgcgc acgaaagacg
      301 gcaaggcgat gaacgctctc attcgcatgc cgcagaagat cgagcgattt atcagtcttc
      361 ccggcagcgg cgatcaagtc attcgtctta tcacgctcga ggccgcgaca gccctgttta
      421 tcggccgatt atttcctggc tatacggtca aaggacatgg cgcgttccgc gcgatcaggg
      481 actccgaagt cgaaatcgag gaagaagctg aagatctggt gcggctcttc gagaccgcct
      541 tgaaacggcg ccgccgtggt tcagttatcc gcctggagct caacgcctcg atgccgggag
      601 agctatgcaa cttcgttcag cgtgcgctgg ctgttgccga cgatgaggtt ttcaaagttg
      661 acggagttct ggcgctcaac gatctcgtcc agctcacaaa actcgatcgc cctgatctcg
      721 agttcgtgcc ctataatccg cgttatccgg agcgcattcg ggatcacggc ggcgattgct
      781 ttgcggcgat ccggcagaaa gatctgatcg tccatcatcc ttacgaatcg ttcgatgtgg
      841 ttgtgcaatt tctgcaacag gccgcaaggg atccgaacgt cgtagccatc aagcagaccc
      901 tttatcgtac gtcggccgaa tccccgatcg tgaagcaact ggtcgaggct gcggaagccg
      961 gaaagtctgt gactgctctt gtcgagctga aagcgcgttt cgatgaggaa gccaatatcc
     1021 gctgggcaag ggacctcgag cgcgcgggcg cgcaggttgt gtacggcttt atcgaactga
     1081 agacgcacgc caaactgtcg ttggtcgtgc gtcgcgaagc cggcgagctc gcgacctatg
     1141 tgcatgtcgg gactggcaac tatcatcccg ttactgcacg catctacacg gatttgtcgt
     1201 tctttaccgc cgacgcggtc atcggccgcg atgtggcgcg catcttcaac tacatcacag
     1261 gttatgcaga accggccgaa ctcgagcgga tggcgatttc tccaatcaat ttgcgcaagc
     1321 ggattctgga ccacatcgcg caggagatcg cgcatgccaa ggcggggcgg ccggcctcaa
     1381 tctggatgaa gattaacgcg ctggttgatc cgggcatcat cgatgccctt tacgatgctt
     1441 ccgccgcggg cgtttccatc gatcttgtcg tccgcggtat ctgctgcctg cggcccggcg
     1501 tgtccggtct gtcggaaaat atcagggtga aatcgatcgt cggcagattc ctggagcatg
     1561 gacgcattta ttgttttggc gcgggtcggg cgctgccgca tccgaaagcg gcggtctata
     1621 tttcttcagc cgacatgatg ccacgcaatc tcgaccggcg cgtcgaggtc ttgtgtccga
     1681 ttctgaatgc caccgtgcac gagcaagttc tcggccagat catggtggcg aacttcaagg
     1741 acaatgagca aagctggcag ctcttgcccg atggcagttc aacgcgcata aaggcggccc
     1801 cgggcgagga gctgttcaac gcccacaaat attttatgac aaatccgagt ctttcgggcc
     1861 gtgggaaatc gctcaaagaa tcctcgccgc gcagccttat acgccgcctc atcgaaggcg
     1921 cgtaagcgtt cgccttccat cgtcccgaag ggccggctcg accatgggcc gccgattgcc
     1981 gttatcgaca tcggttcaaa ctccattcgc ctcgtcgtct acgaaggcct gacacgcgcg
     2041 ccgacaccga tcttcaacga gaaggttctg gcggggctcg gacgacaggt ccagtccacg
     2101 gggttattgc cggctgatgc catcgacacc gcacttggtg cgctgcggcg ttttcgtgca
     2161 ctgtgcaata tcctgcacgt gcggcgcatc tgggcgatcg caacggcggc atgccgggac
     2221 gcgcggaacg ggcgcgcatt catcgccgag gcggaacgca tctgcggcac gcgcatttcg
     2281 attctgtcgg gcaaacgaga ggcccaactc acagcgctcg gagtcgtttc agggattcac
     2341 aatccggacg gtatcgtcgg cgatcttggt ggtggttcgc tcgagcttgt cgatgttcac
     2401 ggaacgcgcg tgcgcggcgg tgtaacgttg ccgctgggcg gcctggcctt gcaggacatc
     2461 accggtaaat cgctgaaaaa ggcggagaga ttcgtcgagg aagccttcga ggacctcgac
     2521 atgctgcacg atggggagga tcgcactttt tacgcggtcg gcggcacctg gcgtgcgttg
     2581 gcgcgcctcc acatgtggca gaccggttat ccattccacg tcatgcacaa ctatcgaatt
     2641 tctgcgcgcg aagcgcagga attctcccgg ctggtgcacc acgtagaccc ggagaccttg
     2701 tcgaagatcg aggtcgtcaa cgcagcgcgc cggccgctgc tcgcctatgc ggcgctggtc
     2761 atggaaaatc ttgttcgcag catcaagccg aaacaggtga ttatttcggt gcttggcgtg
     2821 cgcgagggcc tgctctatgc atcgcttggc gcacgcgagc gcgtacgtga tcctttgatc
     2881 tcggcggcag ccaacctcaa cgtgcttcgc tcacgctcgc caaggcatgg cgaggagctc
     2941 atcgcctgga ccgaccggtt tattgcgtca tcgggtctcg aagagaccgc ggatgaacag
     3001 cggctgcggc acgccgcctg ccttctcggc gatatcggct ggcgcgcgca tcccgactat
     3061 cgcggcgagc agtccatgaa catcattgca aatggtgcct tcgtggcgat cgaccaccct
     3121 gggcgcgctt atctcgcgct gtcagtttac ttccgtcacg ccggtttgaa cgaggaggcc
     3181 ctgtcgcccc ggctgcgcga actcgcgaca acccgcatgc tcgatcgtgc tcgtgtcgtc
     3241 ggagcggcca tgcgcgtcgc ctatatcctg accgccgggc aggccggggt tcttccaaaa
     3301 acaccgatgc aggtcaaacg cggcaagctg attctaaaac tggcgggcgg ttacggcgag
     3361 cttgcaagcg atcggctggc cagccggttg cgccagctgg gacggctggt cggccgtgag
     3421 tctgcgatag aaacgtaaaa ggaataacct ttcgcccctg caactaactc ttctctaccg
     3481 tcacgacttt tcccttgtcg aaggcgagcg ccaggcgtcc gtgcttaagc gcgagtgcct
     3541 tttcgccgaa cagttcgcgg cgccagcctt tcattgccgg aacatcggct tcgtcatcgg
     3601 cggcgatccg atcgaggtcg tcaacggtgg cgatgacctt tgcggcgacg ccgtgccgct
     3661 cggcggtcat ccgaagtaga accttgagca actcgaccgt cgcggcgccg ttcaccgccg
     3721 ggcggaaacg ctccagccgc ggcagcgtct tctgatcgcg ttcgagtccg cgtttgaccg
     3781 cttcgatgat ctgttcaccc cagcgcgagc gctcgaagcc tttcggaagc gagcgcaggt
     3841 ggccaagccg ctccggcgtg gtcggcgcct ggatcgcgat atctccgatg acgtcgtcct
     3901 tgagcacgcg tccgcgcggc acatcgcgta cctgcgcctc gcgctcgcgc caagccgcaa
     3961 cttcgatcag tatggcaagt tctttcggct ttcgaacgcg gctcttcagc cgctcccacg
     4021 cacgttgcgg atcagcccga taggtatcag gcgaggtgag gacacccatt tccgcctcga
     4081 cccagctgct gcggccgcgc ttctcgaggt cctcggcaag tttgagatag acggcgcgca
     4141 gatgggtcac gtcggaaagc gcgtacgcaa tctgcgcgtc gctgagcggc cggcgcgtcc
     4201 agtcggtgaa ccggtgcgac ttgtccagag tgtcaccggt aatacgctgg accagctggt
     4261 cgtaggaaat cgaatcgcca tagccgagga ccatggcggc aacctgcgtg tcgacgatcg
     4321 ggtgggggat cttctgcgcc aggttccaga cgatttcgat gtcctgccgg gcggcgtgga
     4381 agaccttggt gatggtctcg ttcgccatca gctcgaagaa tggagaaagg tcgatgccga
     4441 ctgccagggc atcgatcacg acggcttcat cgggcgaagc catctgcgcc acgcacaaca
     4501 gggggtaata cgtggtctcg cgaaggaatt cggtgtcgac cgtcacgtag gggtgtttcg
     4561 ccgcccgcgc gcaagccgct gcaagttggt cggttgtcgt aataaggtcc attgctgggg
     4621 cgacactaac cccatcaggg tccgtcatgg caacccgctt catgttccgc gcacgcgggg
     4681 catcatgatg aattcccagg ggctcggcat cgccccgacc ggcacctcga tcagcgtcgg
     4741 ctctcgacga gcgattgatt cgcgcagggc gacgcgcaat tcatcgggcc cacgcgcacg
     4801 gcggccagcc gctccgaaac tttccgcata cttgacgaaa tcgggattgg ccagatcgga
     4861 ggcaatcagg cggttgccga attcttcctg ctggatacga cggacattgc caaaagcgcc
     4921 atcaacaaaa ataaccgcca ctaaaggaat gccatgccgg atcgcggtcg caagctcgtt
     4981 gcctgtgtac agaaacccgc cgt
//
LOCUS       DASSRL010000484         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_380372, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000484 DASSRL010000000
VERSION     DASSRL010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380372"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1085
                     /locus_tag="VFL49_09855"
     CDS             228..1085
                     /locus_tag="VFL49_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912325.1"
                     /translation="MAIEPVPIEIDLREPRKNRPDFSTLLWLCGWGVATAVALGALVI
                     TSQTQTANDRLRSIFAVNEPAAVAQMPPRVAQLESETRMLAEQIRALKAERDRLAGRI
                     ALLESSVDDMTGAIKRQAAATAAVLAAKAPALSAAPPAAISAAPSAAVAHQASTTSAP
                     AADPMSTASVPLIPLPPERASAAQAAAEPDTPAASPNGFGLDLGGAPTLDGIRQRWMA
                     IKGDFGPLLSGLHPLAAQERRQGKVSYRLVVGPLPNSPVAAGLCAQFNAAHTACRPAK
                     FHGEQIAQR"
     gene            1118..1492
                     /locus_tag="VFL49_09860"
     CDS             1118..1492
                     /locus_tag="VFL49_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912326.1"
                     /translation="MKLFLPSARATNWLLIVGFCSIGYALYMRYLAVEQSSVGLACQA
                     GLNTWLCFTRQITIVLFTYSVFGWAALIVAALNLIRPSILLFAVALAAACSGIVLYNV
                     GLSSLAAGLLILSFARRAPAPD"
     gene            complement(1458..>2362)
                     /locus_tag="VFL49_09865"
     CDS             complement(1458..>2362)
                     /locus_tag="VFL49_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-(1-6) glucans synthase"
                     /protein_id="HET7912327.1"
                     /translation="GEVGWPSAGRMREGALPSPVNQAKVVQDVLALSKRENFHVNVIE
                     AFDQPWKRALEGTVGGHWGLLDGDSRAPKFGWSLAVSNHPLWIWQGVGGIMFAVAVFG
                     AALYVRDEDAGALRWLGVAANAFVGGALIGWTIENIPIESFGIAGWARSLLLAAVAAS
                     APAVLSMAIMRKSLVPSFAQLLGPREGDGAPLARTAGVILLLVMLLAFVVALGLVFDP
                     RYRDFPFAPLTAAVLPFIVHGFLVGRAKGPRGVAELSGACVLILSAVYILFNEGFANW
                     QSLWVCAVLAALAFNLALVRDARS"
BASE COUNT          466 a          773 c          713 g          410 t
ORIGIN      
        1 gcatgcgcga acgcatgcgc gtcccctcgc cggccgcgag cacgatcgcg aggcatgttc
       61 gacgacgcat tcgatctgga ctcgattcaa ttgccggact gggttgcatc ccaatagccg
      121 aggccgaggc caggaaaaag cctcgtgtgg cccctaggcc gcagagggca aagcgggatt
      181 ggccggattc tgttaatgtt ttcaatgatt cgcccgccga ggacctcatg gcgatcgagc
      241 ccgtgccaat cgagatcgat ttgcgtgagc cacgcaaaaa ccgccctgat ttttcgaccc
      301 tgctgtggtt gtgcggttgg ggcgtggcga cggcggttgc gctcggcgcg ctggtgataa
      361 cgagtcagac ccagactgcg aatgaccgcc tgcggtcaat cttcgccgtt aacgagccag
      421 ccgcagtcgc acaaatgcct ccgcgggtcg cccaactcga atccgaaacc cggatgttgg
      481 ccgagcaaat tcgcgccttg aaggccgagc gtgaccggct cgccgggcgt atcgccctgc
      541 tcgaaagcag cgtcgatgac atgacaggcg ccatcaaacg gcaggcagcc gccaccgcgg
      601 ccgtccttgc ggcaaaggcg ccggctttgt cagcagcgcc tcctgcggca atcagtgccg
      661 caccgtctgc ggccgtcgcg caccaggcct ccacaacgtc tgcgcccgcg gcggacccga
      721 tgagcaccgc ctctgttccg ttgattccct tgccgccgga acgcgcatcg gcggcgcagg
      781 cggccgccga gcctgacacg ccggcagcaa gtccgaacgg attcgggctg gatctcgggg
      841 gcgcaccgac cctggacggc atccggcaac gctggatggc cataaagggc gattttggcc
      901 cgctcttgag cggactgcat ccgctggccg cacaagaacg gcgtcagggc aaagtgagct
      961 accggctcgt ggtcgggccg ctgcctaaca gccccgtagc ggctggcctc tgcgcccaat
     1021 tcaatgccgc gcacacagct tgccggccgg ccaagtttca cggcgaacag atcgcgcagc
     1081 gttgatcgtc acgacggtgc ggccacatac tcatggcatg aaactgttcc ttcccagcgc
     1141 gcgggccacg aattggctgc tgatcgtcgg attctgctcg atcggttatg cgctgtacat
     1201 gcgttacctt gccgtcgagc agtcgagcgt cgggctcgcc tgtcaggccg gcttgaatac
     1261 ctggttatgt ttcacgcgcc agatcaccat cgtgctgttc acttattccg ttttcgggtg
     1321 ggcggcactg attgtcgccg ccctgaattt gataaggccg tcgattttgc tgtttgcggt
     1381 cgcgctggca gcggcctgtt ccggcatcgt gctctacaat gtcggattgt cgtcgcttgc
     1441 cgccgggctg ctgatcctca gcttcgcgcg tcgcgcacca gcgccagatt gaacgccagg
     1501 gccgcgagga cggcgcagac ccacagcgac tgccaattgg caaaaccctc gttaaagagg
     1561 atgtagaccg cggagagaat gagtacgcag gctcccgata gttctgccac gccacgtggg
     1621 ccctttgcgc ggccgaccag aaatccgtga acaataaagg gcaggaccgc cgcggtcagc
     1681 ggcgcaaagg gaaaatcgcg atagcgcgga tcgaacacga ggccgagcgc gacgacaaag
     1741 gccaacaaca tcaccaggag caatatgact ccggcggtgc gagcgagcgg cgcgccatcc
     1801 ccttccctgg gtccaagcaa ttgcgcaaag ctgggaacaa gacttttgcg catgatcgcc
     1861 atactcaata cggctggcgc cgatgccgcg actgcggcga gcaagagtga ccgtgcccac
     1921 cctgcaatgc caaaactctc aatcggaata ttctcaatcg tccatccaat cagcgccccg
     1981 ccgacaaacg cattcgcagc cacgccaagc catcgcagag cgccggcatc ttcgtcgcgg
     2041 acatagagag ccgcgccgaa gaccgcgacg gcaaacatga tgccgccgac accctgccat
     2101 atccacaatg gatgattgga aaccgccagc gaccaaccga atttcggtgc gcgtgagtcg
     2161 ccgtcgagca agccccaatg cccgccgacc gtcccctcaa gcgcacgctt ccacggctgg
     2221 tcaaaagcct cgatgacgtt aacgtggaaa ttctcgcgct tgctgagggc gagaacgtcc
     2281 tggacgacct ttgcctggtt gacaggcgat ggcaaggcgc cctcgcgcat gcggccggca
     2341 ctcggccaac caacttcgcc ta
//
LOCUS       DASSRL010000485         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_47904, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000485 DASSRL010000000
VERSION     DASSRL010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47904"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFL49_09870"
     CDS             complement(<1..795)
                     /locus_tag="VFL49_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7912328.1"
                     /translation="MRIVDIREAAVPLRSTLANSSFDFSEMTTSVVAVITDIVRGGRP
                     VCGFAFNSTGRYACGAQMRARFIPRVLAADTSSLLDASGNNLDPGKILACMMRREKAG
                     GHSERSIAIGTIEVAVWDAVAKIADKPLHVLLAERFNGGKIPEKVFCYVGGGWYWPGQ
                     TVNDLQDEMRRHLGAGYTMVKMKVGGLPLDEDLRRLDAVLHVVGSGEHLAVDANCRFD
                     RNEALRYAKALAPFKLRWFEEPCDPLDFELFAELAEVYEPPLATGEN"
     gene            complement(819..1796)
                     /locus_tag="VFL49_09875"
     CDS             complement(819..1796)
                     /locus_tag="VFL49_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912329.1"
                     /translation="MRLTIRLITAGVTLSMVALGIWPAAAAGYPDHPVKIVVPFAPAG
                     PTDVMARLIAQKLSESLKQQFYIENHPGAGGNIGMTQVAKSPPDGYTILVASSSYVVN
                     PSLYAKNPYDPFKDFAPVTLAAASPNILVVHADFPVKSVKELVDLIKKNPGKYNYAMP
                     GTGTTPHLAGELFKLSFKLDLTTVPFTGAGPAIQSAVAGHTPIAFTALPPTAPQVQAG
                     KLRGLAVTSAKRSSALPDVPTMAEAGVSGQESETMQGILVPAGTPKEIIDVLNREIVK
                     AMALPDVKEKCAQLGFDPIANKPDEFAAYIKKEVEKWGKVIKDAKIPQI"
     gene            1924..>2266
                     /locus_tag="VFL49_09880"
     CDS             1924..>2266
                     /locus_tag="VFL49_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7912330.1"
                     /translation="MPSRKITRQDMRDAAEKYKNWGKWGPDDEIGTLNYTTAEDIVAA
                     TRLVKKGKVISLALNFDHTGPQGAKSKYPSMGRTNPIHTMLRTGTDAYSGVLDKRGIR
                     AADDMVTMPLQC"
BASE COUNT          430 a          691 c          693 g          452 t
ORIGIN      
        1 attttcaccg gtcgccagcg gcggctcgta gacttctgca agctcagcga acagttcgaa
       61 gtcgagcggg tcgcatggct cttcgaacca tcgcagcttg aaaggcgcga gggccttcgc
      121 gtagcgcaaa gcctcattgc gatcgaatct gcaattggcg tcgaccgcaa gatgctcgcc
      181 ggatccaacc acatggagca cggcgtccag gcgccgaaga tcttcgtcga ggggcaggcc
      241 gccgactttc atcttcacca tcgtgtagcc ggcgccaaga tggcgacgca tctcgtcctg
      301 caggtcgttg acggtctgtc ccggccagta ccagccgcca cccacgtagc agaacacctt
      361 ttccggaatt ttgccgccgt tgaaccgttc ggcgagcagc acgtgcagcg gcttgtcggc
      421 aatcttggca accgcatccc agaccgcgac ctcgatggtg ccgatcgcga tcgaccgttc
      481 ggaatggcca ccggcttttt cccgccgcat catgcaagca agaatttttc cagggtccag
      541 attattcccg ctcgcgtcga ggagcgatga tgtatcagcc gcgaggacgc gcggaataaa
      601 ccgcgcgcgc atctgcgcgc cgcacgcata acggcccgtt gaattgaatg cgaaaccgca
      661 gaccggcctg ccgccgcgaa cgatgtcggt gatcacagcg accaccgacg tcgtcatttc
      721 ggaaaaatcg aagctcgaat tggcgagggt cgacctgagc gggaccgccg cctcgcggat
      781 atcgacgata cgcattccgc tatgtcagac agagcgcgct aaatttgtgg gatcttggcg
      841 tccttgatga ccttgcccca cttctcgact tctttcttga tgtaggcggc gaactcgtcc
      901 ggcttgttgg cgatcggatc gaagccaagt tgcgcgcact tctccttgac gtcggggagt
      961 gccatcgcct tgacgatttc ccggttgagc acgtcgatga tttctttcgg cgtgccggcc
     1021 ggcaccagaa ttccctgcat cgtttcggat tcctgcccgc tgacgccggc ctccgccatg
     1081 gtcggcacat ccggcagtgc ggacgagcgt ttggccgagg tgacggccag cccgcgcaat
     1141 tttccggcct gcacctgcgg cgcggtcggc ggcagcgctg tgaaggcgat cggcgtgtgg
     1201 ccggcgaccg ccgactggat cgcgggacct gccccggtga agggaactgt cgtgaggtcg
     1261 agcttgaacg atagcttgaa caactcgccg gccagatgcg gcgtcgtccc ggtgcccggc
     1321 atggcatagt tgtatttgcc gggattcttt ttgatcaggt cgaccagctc cttcaccgac
     1381 ttcaccggga agtcggcatg caccaccaga atgttcggcg aagccgcggc aagcgtgacc
     1441 ggcgcaaagt ccttgaacgg gtcgtacggg ttcttggcgt aaaggctggg attgaccacg
     1501 tagctcgagc tcgcgaccag gatcgtgtag ccgtcgggcg gagactttgc gacctgcgtc
     1561 ataccgatgt tgccgccggc gcccggatga ttttcgatgt aaaactgctg cttcaggctt
     1621 tccgacagtt tctgcgcgat caggcgcgcc atcacgtcgg tcggtccggc gggggcgaac
     1681 ggcaccacga ttttgaccgg atggtccgga tagccagcgg cggcggccgg ccagatgccg
     1741 agcgcaacca tggacagcgt cacaccagcg gtgatgagtc ttatcgttaa gcgcattcgt
     1801 ctcctcccaa ttttttttca tcgtagcatt gcgtgcgaac gttgccagtg gggcttgcat
     1861 cgcccgcgcc cacagggtgt attccggcgg ctttgtcatt ccattttcgt cagaggaaat
     1921 tcaatgccgt cccgaaagat cacccgccag gacatgcgtg atgccgcgga gaagtacaag
     1981 aactggggca aatgggggcc cgatgatgaa attggaacgc tcaattatac gacggccgaa
     2041 gatatcgtcg cggcgacgcg gctcgtgaag aagggcaaag tcatctcgct cgcgctcaat
     2101 ttcgatcaca ccgggccgca gggcgcgaag agcaaatatc cgtcgatggg ccgtacgaat
     2161 ccgatccaca ccatgctgcg gacggggacc gacgcttatt ccggcgtgct cgacaagcgc
     2221 ggcatccgtg ccgccgacga catggtgacg atgcccctcc agtgcg
//
LOCUS       DASSRL010000486         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1674884, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000486 DASSRL010000000
VERSION     DASSRL010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674884"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFL49_09885"
     CDS             <1..890
                     /locus_tag="VFL49_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7912331.1"
                     /translation="SQRFGEPYAVTHRADIHSAFLKACQSSNLITLETSRRVDAYKDH
                     GDSVSVTLDNGETVGGRALIGCDGMWSKIRDQVVGDGKPRVSGHIAYRAVLKRNDVPK
                     DLWRPDVVLWAGPRTHFVHYPLRRGELYNLVAVFHSDHYEEGWNAEGSKDVMWQHFKM
                     QVPQVLRMLERIETWRMWVLCDREPVKNWSQGRVTLLGDAAHPMLQYLAQGACMATED
                     AIVLAEKVAAAPDDPAAAFKAYEQARYLRTGRTQIMARVYGEVYHARGVTAELREMAL
                     SRRTPDENYEGIAWLYGGP"
     gene            complement(1108..2277)
                     /locus_tag="VFL49_09890"
     CDS             complement(1108..2277)
                     /locus_tag="VFL49_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7912332.1"
                     /translation="MAKENKLSVAIVGAGMGGLAVAATLRRVGIDVQVYEQATRFARI
                     GAGIQMMPNSMKVLRGIGVEDRLRATSFAPYSHLNRIADTGEVTRELPMPESLYGAPY
                     LCMHRADLHDALASVVPSEIIHLNKKLVGIDQAGGRVTLRFEDGSAVGADAAIGADGV
                     HSVVRDIVVGPDMPIHKGRIAYRAVFPSKLMGGFDIGRSRTKWWGADRHIVIYYTNKA
                     KREVYFVTSVPEPAEWLTPESWSAKGDVKELRAAYEGFHQDVRNVLNACPDCHKWAIL
                     EREPLRRWSDGRVTLLGDAAHPMTPYMAQGAATSIEDAAILARCLDAVNGDDVEGAFK
                     RYEAHRKPRTSVVQAISSANTWMREGDGDTSWLYGYDAWNANLDQPASNPQMQAA"
     gene            complement(2300..>2620)
                     /locus_tag="VFL49_09895"
     CDS             complement(2300..>2620)
                     /locus_tag="VFL49_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7912333.1"
                     /translation="ILATSSPKPSPLPLLKGIPTVEQAGVKGFEVISWNAIGAPKGTP
                     KEVITTMNKAMHEVLAMPDVKEQFAKVGVVAHASTPEEMMGRLTGDITKWNDVIDKAG
                     IPRK"
BASE COUNT          482 a          825 c          806 g          507 t
ORIGIN      
        1 tctctcagcg gttcggtgag ccgtacgccg taacgcaccg agccgacatt cacagcgcct
       61 tcctcaaggc atgtcagagc agcaatctga tcacgctgga gacgagccgg cgcgtggatg
      121 cctacaagga tcatggggac agcgtcagtg tcacactcga caatggcgaa accgtcgggg
      181 gccgcgcttt gatcggctgc gacggaatgt ggtcgaaaat ccgcgatcag gtggtcggcg
      241 acggaaagcc gcgcgtatcg gggcacatcg cctatcgggc ggtgctcaag cgcaacgacg
      301 tgccgaagga tctgtggcgg cctgatgtcg tgctctgggc cggtccacgc acgcattttg
      361 ttcactatcc gctgcggcgt ggcgagctct acaacctcgt cgctgtcttc cattccgatc
      421 actacgaaga aggatggaac gctgagggca gcaaggacgt gatgtggcag catttcaaga
      481 tgcaggtgcc gcaagtgctg cgcatgctcg agcggatcga gacatggcgt atgtgggtcc
      541 tctgcgaccg cgagccggtg aagaactggt cgcaggggcg cgtcactctg ctcggcgatg
      601 cggcgcaccc aatgctgcaa tatctggcgc aaggcgcctg tatggcgacc gaggacgcga
      661 ttgttctcgc cgaaaaggtt gcagccgcgc ccgacgaccc ggcggccgcc ttcaaagcct
      721 acgagcaggc gcgctacctc cgcaccggcc gcacgcagat catggcgcgc gtctacggcg
      781 aggtttatca cgcgcgcggt gtcaccgccg agttgcgtga aatggcccta tcccggcgca
      841 cgccggacga aaattacgag ggcatcgcct ggctctacgg cgggccgtaa ggaacccgtc
      901 atggccgggc ttgtcaccgc aagtcgggtc tacccgactt gcggcaatta gttatacgga
      961 agtcgggcaa gcccgacttc cgatgccatc cacgtcttcc tttgctgcga tcaagacgtg
     1021 gatgcccggc acaaggcatg tgctcgggcc ggccgaaggc cggacccgag tgccgggcat
     1081 gactattgat tggtaatgcc aagtctacta cgccgcctgc atctgcggat tggacgccgg
     1141 ctgatcaaga ttggcattcc aggcatcgta gccgtagagc cagctggtat cgccatcgcc
     1201 ctcgcgcatc cacgtgttgg cgctggaaat cgcctgcacg acggacgtgc gcggcttgcg
     1261 gtgtgcttcg tagcgcttga atgccccttc aacgtcgtcg ccgttcaccg catcgaggca
     1321 acgcgcaaga atggcggcgt cctcgatcga ggttgcggcg ccctgcgcca tgtaaggcgt
     1381 catcggatgc gcggcgtcgc cgagcaaagt gacgcgcccg tcgctccagc gccgcaacgg
     1441 ctcgcgttcg aggatcgccc acttgtggca atccggacag gcattgagca cgtttcgcac
     1501 atcctgatga aagccttcgt aagcggcgcg cagttccttg acgtcgccct tcgctgacca
     1561 ggattcaggc gtcagccact cggccggctc cggcacgctg gtgacgaagt agacctccct
     1621 cttcgccttg ttggtgtaat agatgacgat gtggcggtcg gcgccccacc attttgtgcg
     1681 cgaacgcccg atgtcgaagc cgcccatcag ctttgacgga aataccgcgc gataggcgat
     1741 gcggcctttg tggatcggca tgtccggccc gaccacaatg tcgcgcacga ccgaatgcac
     1801 cccgtcggca ccgatagcgg catccgcgcc gactgcgctg ccgtcctcga agcgcagcgt
     1861 cacacgaccg ccggcctgat cgatcccgac aagcttttta ttgagatgaa tgatctccga
     1921 tggcacgacg gacgccagtg cgtcatgcag gtcggcgcgg tgcatgcaga gataaggcgc
     1981 accgtagaga ctctccggca taggcaattc gcgcgtcacc tcgccggtat cggcaatgcg
     2041 gttgagatgc gaatagggcg caaacgacgt cgcacgcagg cgatcctcaa cgccgatgcc
     2101 gcgcaggacc ttcatcgaat tcggcatcat ctggatgccg gcgccgatgc gcgcaaagcg
     2161 cgtcgcctgc tcgtagacct ggacgtcaat accgacacgc cgcagcgtgg ccgcaaccgc
     2221 cagtccgccc atcccggcgc cgacgatcgc gactgaaagt ttgttctctt ttgccattct
     2281 ttgtcgttac gcggcgaacc tatttcctcg gaatgccagc cttgtcgatc acgtcattcc
     2341 atttcgtgat gtcgccggtg agcctgccca tcatctcctc gggcgtactg gcatgcgcga
     2401 caacgccgac tttggcgaac tgctccttca catccggcat ggcgagcact tcgtgcatcg
     2461 ccttgttcat ggttgtgatg acttcttttg gcgtgccctt gggcgcaccg atcgcgttcc
     2521 aggagatgac ctcgaagcct ttcacgccgg cctgttcgac tgtcggtatg cccttgagca
     2581 acggtaacgg cgacggcttc ggactcgatg ttgccaggat
//
LOCUS       DASSRL010000487         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1010424, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000487 DASSRL010000000
VERSION     DASSRL010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010424"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFL49_09900"
     CDS             complement(<1..694)
                     /locus_tag="VFL49_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET7912334.1"
                     /translation="MAAPNLSEKIAAANIEAHRRLLASDPVLVDVQLAREALPALADH
                     TILHAGPPIQWDRMCGPMRGAVIGAIVFEGWARNLADAEKLAASGEIRFEPNHHYDGV
                     GPMTGITTRNMAVLVVENRAFGHRAFCTVNEGMGNVMRFGGNDQEVVDRLRWIAAVAG
                     PALGAAIRHRKGIPLKNIIARGLSMGDEMHMRNAACSSLIVRELAADLAETAPKELPR
                     ILRFISGNDMFFL"
     gene            complement(696..983)
                     /locus_tag="VFL49_09905"
     CDS             complement(696..983)
                     /locus_tag="VFL49_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912335.1"
                     /translation="MIYEIGIVIVFVPTDACCRLTVVVGAGIQGRQSMSQQQETEADK
                     LLKSDVRVVNIGLREFARDLSACKVQVIHVDWSPPAVTNPKVASLLAKLGG"
     gene            1085..2194
                     /locus_tag="VFL49_09910"
     CDS             1085..2194
                     /locus_tag="VFL49_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HET7912336.1"
                     /translation="MASGTFEADVICVPMSSPGDVSQVVALFDSKKVDPRHVVAIMEQ
                     TEGDAYARGYATLAMEVLLSERLGISREQVFDTIPMMMIGGVGGIMTPHIYLFIKKPG
                     GSPGKGKRLAIGVANSRELKPEEYGTMVQVDLVADAVREAMKDAGITDPSQVECVEIK
                     CPAMTPARIADAEKRGQKVVAGNPAVASSFAKGACALGVAVATGEVERGKLKDGMINI
                     EKSLYSSVASTSAGGEQVACRVMVIGNVEGAPGRLVATHGVMQDQLDIVGARKPFAQA
                     GLEMKDGVVTEADRERIAGVLVNAGADYVTNVRGRRHVLHSDFLAPFSGIQAKAVAHA
                     IVGTIVGDTLFLASAGAEHQGPAGANLICVIAEAK"
     gene            2209..2631
                     /locus_tag="VFL49_09915"
     CDS             2209..2631
                     /locus_tag="VFL49_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7912337.1"
                     /translation="MDVEKHLRLTHAGAMKLIEAGVKKAVEMKKPQCIAVVDEGGHLI
                     AFTRMDGGKVLSIESAIRKAQTAASGRGPTGGIQGDLEFRLAHATSGRLVNLKGGLPV
                     IVDNVTIGGIGVGSQTGEEDLEVAKAALHALAGAKTFD"
     gene            2681..>2985
                     /locus_tag="VFL49_09920"
     CDS             2681..>2985
                     /locus_tag="VFL49_09920"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7912338.1"
                     /translation="MMKMAVFCGSNFGASGVYREAGVALGRALAAEKIQLVFGGTSKG
                     LMKIIADTVLENGGSVHGIITQGLVDRGQQYPGLTVPEIVATRSLRKIRMAEVSDGFL
                     "
BASE COUNT          556 a          890 c         1000 g          539 t
ORIGIN      
        1 tgaggaaaaa catgtcgttg ccggagatga agcgcaggat acggggaagc tccttcggcg
       61 ccgtctccgc aaggtcggcg gcgagttcgc gcacgatcag actcgagcac gcggcgttgc
      121 gcatgtgcat ctcgtcgccc atcgacaggc cgcgcgcgat gatgttcttg agcggaatgc
      181 ccttgcggtg ccggatagcg gcgccgagcg ccgggccggc gacggcggcg atccacctga
      241 ggcggtccac gacctcctgg tcgttgccgc cgaatcgcat gacgttgccc atgccttcgt
      301 tgacggtgca gaaggcgcgg tgtccgaaag cgcgattttc caccaccagg accgccatgt
      361 tgcgcgtggt gatacctgtc atcggcccga cgccatcata gtgatggttc ggttcgaacc
      421 ggatctcacc ggaggccgct agcttttccg cgtccgcgag attgcgcgcc cagccttcga
      481 acacgatggc gccgatgacc gcgccgcgca tcggcccgca catgcgatcc cattggatcg
      541 gcgggccggc gtgtaaaatc gtgtggtcgg ccagcgccgg gagggcctcg cgcgcgagct
      601 gcacgtcgac gagcacgggg tcgctggcga gcagccggcg atgcgcttcg atgttggcgg
      661 cggcgatttt ctcgctcaaa ttcggcgcgg ccatttcagc ctccgagctt ggcgagcagg
      721 ctggcgacct ttggattggt tacagctggg ggcgaccaat cgacgtggat aacctggacc
      781 ttgcaggctg aaaggtcgcg cgcgaattca cgcagtccga tattgacgac gcgcacgtcc
      841 gacttcagca gtttgtcggc ttcggtttcc tgctgttgcg acatgctttg ccttccctgt
      901 ataccggccc cgacaacgac ggtcagcctg caacaggcat cggtcggcac gaaaacgata
      961 acaatgccga tctcatagat caatcagatt ttcgaaatta ttggcaaagg atggagcccc
     1021 ggatatagtg ccggccgccg ctggtcattc cgccgcgcgg tgccgggaca ggagaagaca
     1081 cttcatggcc agtgggacgt ttgaagccga tgtgatttgc gtgccgatga gctcgccggg
     1141 cgacgtatcg caggtcgtcg cattgttcga cagcaaaaaa gtcgacccga ggcacgtcgt
     1201 cgcgatcatg gagcagaccg aaggcgacgc ctatgcgcgc ggctacgcga cgctggcgat
     1261 ggaagtcctg ctttcagagc ggctcggaat ttcgcgcgag caggttttcg acaccatccc
     1321 catgatgatg atcggtggcg ttggcggtat catgacgccg cacatctatc tgttcatcaa
     1381 aaagcccggc ggtagtccgg gcaaaggcaa gcgactcgcg atcggcgtcg ccaactcgcg
     1441 cgagctcaag ccggaagaat acggcaccat ggttcaggtc gacctcgtgg ccgacgcggt
     1501 gcgcgaagcc atgaaggacg ctggcataac cgatccgtcg caggtcgagt gcgtcgaaat
     1561 caaatgcccg gcgatgacgc cggcgcgcat cgccgatgcc gagaagcgtg gccagaaggt
     1621 cgttgccggc aatccggcgg tggcttcctc gttcgccaag ggcgcttgcg cgctcggcgt
     1681 cgcggtagcg accggcgagg tggaacgcgg aaagctcaag gatgggatga tcaatatcga
     1741 gaagtcgctg tactcgtcgg tggcctcgac ctcggccggc ggcgagcagg ttgcctgtcg
     1801 tgtaatggtg atcggcaacg tcgaaggcgc accgggcagg ctggttgcca cgcacggcgt
     1861 gatgcaggat cagttggaca tcgtgggagc acggaagcct ttcgcgcagg ctggccttga
     1921 aatgaaagac ggcgtcgtga ccgaagccga ccgcgagcgg atcgcgggcg tgctggtgaa
     1981 cgccggcgcc gattacgtca ccaatgtgcg cggccgccgt cacgtcctgc attcggattt
     2041 cctcgcgcca ttctccggca tccaggccaa ggccgtcgcc catgcgatcg tcgggacgat
     2101 cgtcggcgat accttgttcc tcgcctcggc tggcgcggag catcagggcc cggccggcgc
     2161 caatctcatc tgcgtcatcg cggaagcaaa atagctcgga gacagctcat ggatgtcgag
     2221 aagcacctgc gcctgacgca tgctggcgcg atgaagctca tcgaggccgg cgtcaagaaa
     2281 gcggtcgaga tgaagaagcc gcaatgcatc gcggtggtcg acgagggcgg tcatctgatc
     2341 gcattcacgc gtatggatgg cggcaaggtg ttgagcatcg agtcggcaat ccgcaaggcg
     2401 cagaccgcgg cctccggccg cggaccgaca ggcggcatcc agggcgatct cgaattccgc
     2461 ctcgcgcatg cgacgtcggg ccgtctcgtc aatctcaagg gcggcctgcc tgttatcgtc
     2521 gacaacgtca ccatcggcgg catcggcgtc ggctcacaaa ccggcgagga ggatctcgag
     2581 gttgccaagg ccgctctgca cgcgctcgcg ggcgcaaaga ctttcgactg aggcgcgctt
     2641 ttgacggcgc tgttcgcggc ggcaaacccg agatcctcgc atgatgaaga tggcggtgtt
     2701 ctgcggctcg aacttcggcg cgtccggggt ttaccgtgag gccggcgttg cgcttggccg
     2761 cgcgctggcg gctgagaaaa tccagctcgt gttcggcggc accagcaaag ggctgatgaa
     2821 gatcattgcc gatacggtgc tggaaaatgg cggctcagtg catggcatca tcacgcaggg
     2881 cctggtcgac aggggccagc aatatccggg cctgaccgtg ccggagatcg tggcgacccg
     2941 ttcactgcgt aaaatccgca tggcggaagt atcggacgga tttct
//
LOCUS       DASSRL010000488         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_485301, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000488 DASSRL010000000
VERSION     DASSRL010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485301"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFL49_09925"
     CDS             complement(<1..1012)
                     /locus_tag="VFL49_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET7912339.1"
                     /translation="MHCRVRAGVAAALVALMLSPALAADKAFKQDALDEAAIKLEAQI
                     KSDAGHVGKPAATLRRDADAAFQKNDFRAGMVVLGQLVAAAPDDATSWLRLSRAVLQI
                     KPRDDREKALLLDRASTAAYIAYERASDRNVEADSLSVLGRTLADRQQWRAALDSLRL
                     SLDLRETAELRAQYEKLRAEHGFRLLDYTVDSDSISPRACFQFSEELPGRRTDFSAFV
                     AVAGIERPAIAASEKQLCVEGLKHGERYSVTLRAGLPSVVKETLAKSAEFTIFVRDRK
                     PFVRFSGKAYVLPRTGQRGIPVLSVNTRAVTLSIYRIGDRNLVDTLLGYDFQRNLSRY
                     QQE"
     gene            1362..1438
                     /locus_tag="VFL49_09930"
     tRNA            1362..1438
                     /locus_tag="VFL49_09930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1396..1398,aa:Arg,seq:tct)
     gene            complement(1666..1851)
                     /locus_tag="VFL49_09935"
     CDS             complement(1666..1851)
                     /locus_tag="VFL49_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912340.1"
                     /translation="MASVFTDWTSRAAIASRIAEERQSPVESWKELTVPEDYDLSFDK
                     IEYQIRPAKWPWLVYII"
BASE COUNT          439 a          634 c          678 g          412 t
ORIGIN      
        1 gttcctgctg atagcggctc aggttgcgct ggaagtcgta gccgagaagc gtgtcgacca
       61 gattgcggtc gccgatccgg tagatcgaca gcgtcaccgc ccgtgtgttg acgctgagca
      121 ccggaattcc gcgctggccg gtgcgcggca gcacataggc cttgccggaa aaccgtacga
      181 agggctttcg gtcacgcacg aagatcgtga actcggccga ctttgcgagc gtctccttga
      241 cgacggatgg caagcccgcg cggagcgtca ccgaatagcg ttccccgtgc ttgaggcctt
      301 cgacgcaaag ctgcttttca ctcgcggcaa tggccggcct ctcgatgccg gcgacagcga
      361 caaaggcaga aaagtcggtg cgccgtcccg gcagttcctc ggagaactgg aagcaggcgc
      421 gaggcgagat cgaatcagaa tcgaccgtat agtcgagcag cctgaacccg tgctcggccc
      481 gcaatttttc atattgggcg cgcagttcgg cggtttctct caagtcgagc gagaggcgta
      541 gggaatcgag cgcggcgcgc cattgctggc ggtccgccaa ggtgcggccg agcaccgaca
      601 ggctgtcggc ttcgacgttg cggtcgcttg cgcgctcgta cgcgatataa gcggccgtcg
      661 aggcgcggtc gagcagcagc gccttctcgc ggtcgtcgcg cggcttgatc tgcagcacgg
      721 cgcgggatag ccgcagccag ctcgtcgcat cgtccggtgc agcggccacg agctggccga
      781 gcaccaccat gccggcgcgg aaatcattct tctgaaaggc ggcatcggca tcgcgacgga
      841 gcgtggcggc aggcttgccg acgtggccgg cgtcgctttt gatctgtgcc tcaagcttga
      901 tcgccgcttc gtcgagagcg tcttgcttga acgctttgtc cgcggcgagc gcaggcgaca
      961 gcataagggc aacgagcgcg gcggcgacgc cggcgcgaac gcgacagtgc atggaacgag
     1021 acctccggag gcagcggatc gccgcacctc ttatttcagg gcctgatggt gtcgaggaca
     1081 tggccgaacg attgcgagac ctttgccagt ggatagcggc atgcgggctg gatccctgaa
     1141 ttgatacgat gatcaattgg ctcggccaat atcctcaaat gccacatcat caacttaaag
     1201 aattacccta agggtttaat ctaacaatag aaatcggcgt cgccttgtcg cgaggcgcgc
     1261 accgtcacgt acgaccttcg ttgtgctgtc gaaattgttg ttgccagccg gcaaccgggc
     1321 ttttgcgccg gccgcccgac gcaggtaaac cgtgttgcga cgaccccgta gctcagccgg
     1381 atagagcatc agccttctaa gctgagggtc gctggttcga atccagccgg ggtcgccagt
     1441 tctttgaggt ccggcccgga gacatgggtg acagaacgta cctaaggcat gggtgacaac
     1501 cattgaagca gactcgcccg catgtggtga cgatgacgat tttgtcatgg aacggatagt
     1561 cgaagcccgg caagcccggt gtggcgtcgc actctgctga cggcggcgac gattcatgcg
     1621 gacaaacgca taaactgttg cgttcctggc gcgccttgct ttcgatcaga tgatgtagac
     1681 gagccaaggc cacttcgcag gccttatctg atactcgatc ttgtcaaagg acaggtcata
     1741 atcttccggg accgtcagtt ccttccagct ttcgaccggg ctctgccgtt cttccgcgat
     1801 gcgcgaagcg atagcagccc ggctcgtcca atccgtaaaa acgctcgcca tggggacctc
     1861 caatcgcttg gagttcacgt gtctcatggg caacctaaga tttcgcccga ggtccgcgtg
     1921 taaggaggtt cacagagatg acaaatttgt aagcgcgccg gagaagagag gaagcgcagc
     1981 tggaagcttg tacgcaagta cccgagatcg caggtctaaa cgatgcgctc gttgctgatc
     2041 gcccggcaat ccatttcaaa atcgagatca accacagggg gacggcaaaa gcaccaggtc
     2101 agcccgcctt cggccgcccc ccggcgaaca atctcgtccg caagaaacgg gtaaaggtcg
     2161 tgc
//
LOCUS       DASSRL010000489         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1062745, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000489 DASSRL010000000
VERSION     DASSRL010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062745"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VFL49_09940"
     CDS             <1..1222
                     /locus_tag="VFL49_09940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7912341.1"
                     /translation="GRLLDLMNQGQVRFDGLEAFVLDEADRMLDMGFILPVKRIVAKL
                     PKQRQTLLFSATMPQDVAGLAAGLLADPVRVEVTPVATTAERISQRVHFVEKANKRAL
                     LHHVLSNQEVTRALVFTRTKHGANKVGDQLERLGVRADVIHGNKSQNARQKALEDFRN
                     GKVRVLVATDIAARGIDVDGITHVINFDLPNEPESYVHRIGRTARAGNEGIAISFCDS
                     EERAFLRDIEKTIRRQVAVDADHPFHAAHIAEGGRSANARGANPGGGGVRRNRDGNNG
                     GSRDQAADERHGKHGVRGAASKAGAQRRHRGGKNHAHGGIGNPHDLPPQERHGRPRSG
                     ERDHAHAKEPRHGGSHRHEASNKGQQHPQKSAGGDPRQANGGNQFLRRSRQGRSNPNS
                     AAARPQRFEPVRMG"
     gene            complement(1297..>2073)
                     /locus_tag="VFL49_09945"
     CDS             complement(1297..>2073)
                     /locus_tag="VFL49_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7912342.1"
                     /translation="QAEDALRRAQMDAEIANRAKSQFLANVSHELRTPLNAIIGFSEI
                     MNGEMMGPLGSSTYKEYASDIHESGRHLLAIINDILDLSRVEAGQVTLNESAIEIEKL
                     LGACLILVRGRANEGGLTLSVEATPATPEILGDERLLKQALLNLLSNAIKFTPRGGAI
                     RIATRVSRIGLEIAVSDSGIGMNESELAKVAKPFVQLENWLVRKYEGTGLGLSIAKAF
                     CELHGGRLEIVSAPGKGTTATIHLPASRIASVGRVRARAR"
BASE COUNT          390 a          675 c          643 g          365 t
ORIGIN      
        1 gggccggctt ctcgatctga tgaaccaggg ccaggtgcgg ttcgatgggc tcgaagcctt
       61 cgtgcttgac gaagccgacc gcatgttgga catgggtttc atcctgccgg tcaagcggat
      121 cgttgccaag cttcccaagc agcgccagac gctgcttttc tccgcgacca tgccccagga
      181 tgttgcaggg ctcgcggccg gcttgttggc cgatcccgtt cgggtggagg tcacgccggt
      241 agccaccacc gccgagcgga tctcccagcg cgtccacttt gtcgagaaag cgaacaagcg
      301 cgcgctcctg catcacgtgt tgagcaatca ggaggtcacg cgtgccttgg tcttcacccg
      361 gaccaagcac ggcgcgaaca aggtcggcga tcaactggag cggctcggcg ttcgcgccga
      421 cgtcatccac ggcaacaagt cgcagaatgc gcgtcagaag gcgttggagg atttccgcaa
      481 cggcaaggta cgcgttctgg tggccaccga catcgccgcc cgcggcatcg atgtcgacgg
      541 catcacacac gtcatcaact ttgacctccc gaacgaaccc gagagctatg tccatcgcat
      601 cggtcgcacg gcacgggcgg gcaacgaagg catcgcgatt tcattctgcg attccgaaga
      661 acgggcgttc ctgcgtgaca tcgagaaaac gatccgccgg caggtcgccg tcgacgccga
      721 tcatcctttc cacgcagcgc atatcgccga aggcggccgc tcggcgaatg ctcggggcgc
      781 caaccctggc ggaggaggcg tccggcggaa ccgagatggc aacaacggcg gttcgaggga
      841 tcaggccgct gacgaaaggc atggaaaaca cggtgtccgc ggggcagcgt cgaaagccgg
      901 cgcccagcgc aggcatcgcg gcggcaagaa ccatgcccat ggcggcatcg ggaatccgca
      961 tgacctgccg ccccaggagc ggcatggccg gcctcggagc ggagagcgcg accatgctca
     1021 cgcgaaggaa ccgcgccacg ggggcagcca ccgtcacgaa gcatcgaaca aggggcagca
     1081 gcaccctcag aagtcggccg gcggggatcc gcggcaggcg aacggcggca accagtttct
     1141 aagacgttcg cgtcagggac ggagcaatcc caactccgcc gctgcgcgtc cgcagcgatt
     1201 cgaacctgtg cgtatggggt agctcgttga cgttgcccgg ctcccagtgc gatctgcacc
     1261 gtcggcgaac cgctggcggt gcagcgctgg ccttcttcac cgagctcgtg cgcggactct
     1321 gccgaccgaa gcgattcgcg atgcgggcag atgaatggtc gccgtcgtgc ccttgccggg
     1381 agcgctgacg atctccagcc ttccaccatg cagctcgcag aatgccttcg caatggacag
     1441 accgaggccg gtgccctcgt acttgcgcac cagccagttc tcgagctgaa caaaaggctt
     1501 ggcaaccttc gccaactcgc tttcattcat cccgattcct gaatcggaaa cagcgatttc
     1561 caggccgatc ctgcttaccc gcgtcgcgat ccgtatcgcg ccgcctctcg gggtgaactt
     1621 gatcgcgttg gaaagcagat tgagcaacgc ctgcttcagc agccgttcgt caccgaggat
     1681 ctccggcgtg gccggggtcg cctcgaccga caacgtcaat ccgccttcat tcgctctgcc
     1741 acgtaccagg atcaggcatg cccccaacag cttctcgatc tctatcgcgg attcgttgag
     1801 cgtgacctgt cctgcctcga cgcggctgag atccaggatg tcgttgatga tggcaagcag
     1861 gtgccgaccg ctctcgtgga tgtcgctggc atactccttg taagtcgagg agccaagcgg
     1921 ccccatcatc tctccgttca tgatctccga gaagccgatg atcgcattca acggcgtccg
     1981 cagttcgtgg cttacgttgg ccaggaactg ggacttggcc cgattggcga tctccgcatc
     2041 catttgggcg cgccgcaggg catcctctgc ctg
//
LOCUS       DASSRL010000490         4365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_765269, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000490 DASSRL010000000
VERSION     DASSRL010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765269"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFL49_09950"
     CDS             <1..357
                     /locus_tag="VFL49_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HET7912343.1"
                     /translation="AWNLINASSRGLLPFARLVGDDRVQVAHRSGRLLVRPAEALAGL
                     IEIYGLGIRTLTFEAVAVVGWIVDLSAADATRHPDGKVAATAVAEVALPRIAVAAGMD
                     PLPVVLAALRTSPAGD"
     gene            595..996
                     /locus_tag="VFL49_09955"
     CDS             595..996
                     /locus_tag="VFL49_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7912344.1"
                     /translation="MIGLVLVTHGRLAIEFRSALEHVVGPQRQIEAITIGPDDDVEQC
                     RKNILEAVKRVDSGEGVAILTDMFGGTPSNLAISVMSQPKVEVLAGINLPMLIKLAKI
                     REISPLQDAVAAAQEAGRKYCTIASRVLNGK"
     gene            993..1292
                     /locus_tag="VFL49_09960"
     CDS             993..1292
                     /locus_tag="VFL49_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET7912345.1"
                     /translation="MSPRTSVDETPVVREVEIVNKKGLHARASAKFVQTAEQFDAAIT
                     VTRGHETVGGTSIMGLMMLAAGPGISITIKAIGKDAAAAVETLSKLVQDGFGEED"
     gene            1599..2882
                     /gene="soxC"
                     /locus_tag="VFL49_09965"
     CDS             1599..2882
                     /gene="soxC"
                     /locus_tag="VFL49_09965"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HET7912346.1"
                     /translation="MFPSRATANRRNFLFAAGSSAGVGAAVLVVGSAAAQQAPAGAKH
                     FDVPADPTKEQGRPVAADGGYGTRSQFETEVRVRFPTANENTSWSFTPLDQSLGNLTP
                     SGLHFERHHGGIPTIDPSKHMLYVHGMVDGPRKYTMADLKRFPSVTRKYFIECSGNGL
                     TEWNKPTLKTVQGTHGLLSTSEWTGVQFSTIAREVGLKDGGAWVLAEGADAAVMTRSI
                     PMEKMLKDAILAYGQNGEAIRPEQGYPLRLLLPGYEGNTHIKWLRRLEVSDKPFMTRE
                     ETSKYTDLLANGKARQFSFDMEAKSVITFPSGEMKLPGPGFYNISGLAWSGRGRVQSV
                     DVSTDGGATWVPARIESVPEPICTVRFSLPWIWNGQPAILQSRCTDETGYIQPTLKQL
                     IAIRGSNGPFGSIYHLNAIQSWGVAADGSVTNVHA"
     gene            2869..3432
                     /locus_tag="VFL49_09970"
     CDS             2869..3432
                     /locus_tag="VFL49_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7912347.1"
                     /translation="MSTRKLGFATALIGALAFAHTASAQVAHYGFGSAATPAELSRFF
                     AIPPDGRGLPPGSGNAAKGAQIYAGACAACHGDKLEGNPAKGSGGDKLLGGRGTLASK
                     TPVKTVESYWPYATTLFDYVKRAMPFNAPGSLSDDDVYSVVAYMLSEAKIIQPNDTMD
                     AKSLSKVAMPNRDGFEPDPRPELQLYR"
     gene            3575..>4365
                     /locus_tag="VFL49_09975"
     CDS             3575..>4365
                     /locus_tag="VFL49_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7912348.1"
                     /translation="MSAKVASEYTVKDIGLADFGRKEISIAETEMPGLMATREEYGPK
                     QSLKGARIAGSLHMTIQTAVLIETLKALGADVRWVSCNIYSTQDHAAAAVAAGGTPVF
                     AVKGETLKDYWDYTRKLFEWHGGGYPNMILDDGGDATLFLHLGVRAEGGDTGFLDKAT
                     SEEEEILFALIKKTLKEKPKGWFGEIAKSVKGVSEETTTGVHRLYEMQKKGTLLFPAI
                     NVNDSVTKSKFDNLYGCRESLVDGIRRGTDVMMAGKIAMVAGFGDV"
BASE COUNT          904 a         1357 c         1355 g          749 t
ORIGIN      
        1 gcctggaatc tcatcaatgc ttcgtcgcgg ggcttgctgc catttgcccg tcttgtcggc
       61 gacgaccggg tccaggtggc acaccgatcg ggacggctgc tggtgcggcc cgcggaggcg
      121 ctcgccggtc ttatcgaaat ttacggcctt ggcatccgca cactgacatt tgaagccgtc
      181 gccgtcgtcg gctggatcgt tgacctgagc gcggcagatg ccacgcgtca cccggacggc
      241 aaggtcgccg cgaccgccgt cgccgaagtc gcgctcccgc ggattgcggt tgcggccgga
      301 atggatcccc taccggtggt gttggccgcc ctgaggacat ccccggcggg cgattgacct
      361 tccgcggaaa tatgggctac ccccggcccc ccgacctgac cgagaaaatt aaccatacag
      421 ggcctaggat cgcgcaccgt caaaaacgca cgttccgagc ccactttggc cccacctttc
      481 cgcagtgcag cagaacgcct atatagcgtg ccgagccctt gcaatagggc ctctgatggg
      541 cgtgaattcg cgccctatcg tgcggcgcag cgaagcgccc gcgaggagcc agggatgatc
      601 ggcctcgtac ttgtgaccca cgggcggctc gccatcgagt ttcgctcagc gctggaacac
      661 gtggtgggac cccagcgtca gatcgaggcc attacaatcg ggcccgacga cgacgttgaa
      721 cagtgccgca agaacatcct tgaggcggtg aagcgggtcg atagcggcga gggcgtcgcg
      781 atcctgaccg atatgttcgg cggcacgccg tccaatctcg ccatttcggt gatgagccag
      841 cccaaggtcg aggttctggc cggtatcaat ctaccgatgc tgatcaagct cgccaaaatt
      901 cgcgagatca gtcctttgca agacgcggtc gccgccgctc aggaagcggg ccgcaagtac
      961 tgtacgatcg ccagccgcgt cctcaacgga aaatgagtcc ccgcacgtca gtggacgaga
     1021 ctcctgtcgt ccgcgaggtg gagatcgtca acaaaaaggg attgcacgcc cgcgcttctg
     1081 cgaaattcgt gcagactgcg gagcaattcg atgccgctat tacggtaacc cgcggccatg
     1141 aaacggtcgg cggaacatcg atcatgggac tgatgatgct ggctgccggt cccgggattt
     1201 ccatcacgat caaagcgatc ggcaaggacg cggcggccgc ggtcgagaca ctgtccaagc
     1261 tggttcagga tggcttcggc gaagaagact gactgacggc accgcgggcg gccggagaat
     1321 tttcgaagac aacaattccg atcgcacgtc gcgtggttgc agacatcggc ggattcaccc
     1381 caacaaaata actttccggc gaggggagcc gtgcctaaag tcaaggcacg ggcacgatgg
     1441 acggttgccc gtaacccaag gaggcgtcct tgagcacgac ccctatcccc acaatcgatg
     1501 accaaacgca gcgcatcatc gatggcatga tcgccgctga atcgcgtcac ctctcgcgac
     1561 gcgccgccga aaaagaagcg tgtgaatgta aggacgggat gtttccgtcc cgcgcaacag
     1621 ccaatcggcg caattttctc tttgcggccg gatcgagtgc cggcgtcggg gcagctgtgc
     1681 tggtcgtcgg ttccgccgcc gcacaacagg cgccggctgg cgccaagcat ttcgatgtgc
     1741 ccgcggaccc gaccaaagag cagggtcgcc cggttgcggc tgacggtgga tatggcacgc
     1801 gttcgcaatt cgagaccgaa gtgcgcgtgc gtttcccaac cgcaaacgaa aatacatcgt
     1861 ggagcttcac cccgctcgac cagagcctgg gcaatctcac gccgtcgggc cttcacttcg
     1921 agcgccacca tggcggaatc cccacaatcg atccgtccaa acatatgctt tacgtgcacg
     1981 gcatggtcga cgggccgcgc aagtacacga tggcggattt gaagcgattt ccgtcggtca
     2041 cgcgcaagta cttcatcgag tgctcgggca atggcctgac cgaatggaac aagccgacgc
     2101 tgaagacggt ccagggcacg cacggcttgt tgagcacgtc ggaatggaca ggtgttcaat
     2161 tctcgaccat cgcgcgcgaa gtcggcctca aggacggcgg cgcgtgggtg ctggcggagg
     2221 gcgccgatgc cgccgtgatg acgcgcagca tcccgatgga gaagatgctc aaggacgcga
     2281 tcctcgccta cgggcaaaac ggcgaagcga tccggcccga gcagggctat ccgctgcgac
     2341 tgctgctccc cggttacgaa ggcaacaccc atatcaaatg gctgcgccgc cttgaggtca
     2401 gcgacaagcc gttcatgacg cgcgaggaga cgtcgaaata caccgacctc ctcgccaacg
     2461 gcaaagcgcg tcaattcagc ttcgacatgg aggcaaagtc ggtcattacg tttccgtccg
     2521 gggaaatgaa gctgccgggt cccggcttct acaacatcag cgggcttgcc tggtccgggc
     2581 gtggccgggt gcaatccgtc gacgtgtcga ccgacggcgg tgcgacctgg gtgccggcgc
     2641 gcatcgaatc cgtgccggaa ccgatctgca cggtgcgctt cagcttgccc tggatatgga
     2701 acgggcagcc ggccattctg caaagccggt gcaccgacga gaccggctac atccagccga
     2761 cgttgaagca gcttatcgcg atccgcggct cgaacgggcc gttcggctcg atctatcacc
     2821 tcaatgccat ccagagctgg ggcgttgcag ccgacgggag cgtcaccaat gtccacgcgt
     2881 aagcttggct ttgcgacggc cctaatcggc gcactcgcat tcgcgcacac agcctcggcg
     2941 caggtcgcgc attacggctt tggttcggcg gcaacgccag ctgaactgtc caggttcttc
     3001 gcgattccgc cggatggccg aggccttccg ccgggcagcg gcaatgccgc caaaggcgcg
     3061 caaatctacg caggcgcttg cgcggcctgc catggcgaca agcttgaggg taacccggcc
     3121 aagggcagcg gcggtgataa actgctcggc gggcgcggca cactcgccag caagacgccg
     3181 gtgaagacgg tcgaaagcta ctggccctac gccacgacgc tgttcgacta cgtcaagcgc
     3241 gccatgccgt tcaacgcgcc gggctccctc agtgatgacg atgtctacag cgtcgtggcc
     3301 tacatgctgt cggaagcgaa gatcatccag ccgaacgata cgatggacgc caagtcgctt
     3361 tcaaaggtgg ccatgcctaa cagagacggg ttcgagccgg acccacgtcc cgaactgcag
     3421 ctctatcgct agaaatctgg ccggaagggc cggaaaccgg caattccggc ccctccatcg
     3481 tacataaaga attctttata tatgcattgc ggcgtcggcg gcgggctgtt ataggcacgc
     3541 cagccaagca atcccctatg agccaggaac cgtcatgagc gcgaaagtag cgtccgaata
     3601 caccgtcaaa gacatcggcc tcgccgattt cggccggaag gaaatttcca tcgccgaaac
     3661 tgaaatgccc ggcctgatgg caacgcgcga ggaatacgga ccgaagcagt cgctcaaagg
     3721 cgcgcgcatc gccggctcgc tgcatatgac gatccagacg gcggtgctga tcgagacgct
     3781 caaggcactc ggcgccgacg tgcgctgggt ctcgtgcaac atctactcga cgcaggatca
     3841 cgccgccgcg gcagtggcgg ccggcggcac gccggtcttc gccgtcaagg gcgagacgct
     3901 gaaagattac tgggactaca cccgcaagct gttcgagtgg cacggcggcg gctatccgaa
     3961 catgattctg gatgatggcg gtgatgcaac cttgttcttg catctcggcg tgcgcgccga
     4021 aggcggcgac accgggttcc tggacaaagc cacctcggag gaagaggaaa tcctcttcgc
     4081 cctgatcaag aagacgctga aggaaaaacc gaagggttgg ttcggcgaga ttgcgaagtc
     4141 ggtcaaaggc gtctcggaag aaaccaccac cggcgtgcat cgcctctacg agatgcagaa
     4201 gaagggcacc ctgctctttc cggcaatcaa cgtgaacgac tccgtcacca agtcgaaatt
     4261 cgacaatctc tacggttgcc gcgagtcact ggtcgacggc attcgccgcg gcaccgacgt
     4321 gatgatggcc ggcaagatcg cgatggtcgc cggcttcggc gatgt
//
LOCUS       DASSRL010000491         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_275193, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000491 DASSRL010000000
VERSION     DASSRL010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275193"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..971)
                     /locus_tag="VFL49_09980"
     CDS             complement(105..971)
                     /locus_tag="VFL49_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912349.1"
                     /translation="MKSEATFTGVLGVALALVMLSAGAPVAAAADMIGNCELTGTKGE
                     FPFTPAKAGQLTVEVNLPAPGWWNGDTPETIKDGYEYCLAANIAHRAGFDKVEVVNVA
                     WDALVAGQTRDFDLALSEISVTAERKKVVDFSVPYFSSDIGVMVKKGTKVDGNSIRDM
                     RIGVQQATTGAAFVDQQLKPKTPASVYPDTPSMFTALQAGQIDVAMTDTAIVLAQAGT
                     SNGVFEVVGQYATGETYGALYPKGSKNEKVIDQIIESLKKDGTLDKLSAKYLAAAWGA
                     DPTKVPYFKPAM"
     gene            complement(1141..1413)
                     /locus_tag="VFL49_09985"
     CDS             complement(1141..1413)
                     /locus_tag="VFL49_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HET7912350.1"
                     /translation="MALPVRDLSDSGRGPVKRLTAPRHVGIEPKEQKILALKSSVHFR
                     ADFQPIAEEILIVAAPGANPVDNRDLPYKRIRKGIRLMPLAEPFGG"
     gene            complement(1561..>2068)
                     /locus_tag="VFL49_09990"
     CDS             complement(1561..>2068)
                     /locus_tag="VFL49_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET7912351.1"
                     /translation="RKLAIRAFAIASVTTLLAAAIGRAILENWHVSLGALLIAGGVIL
                     FLVALRQVLQQYAPASEIEARQPEPALAGMAIRLAFPTIVTPYGIAAVIILLSVSPNA
                     GYVIGVAAALIGVMVLNLLAMLYARKILKVIGLMPLQIVGTVLSVMQVALGVQMILGG
                     LRMIGVLQ"
BASE COUNT          385 a          638 c          661 g          384 t
ORIGIN      
        1 gcgagcgccc cccagctcag acgggaagga tccttcggaa gcgaaggggc gcgccatctt
       61 cggccgcgcg ccccctcctc caaggggatc ttgctcaccg ccgactacat cgccggcttg
      121 aagtatggca ccttggtggg gtcggcgccc cacgccgcgg cgaggtactt ggcggacagc
      181 ttgtcgagcg tgccgtcctt cttcagggac tcgatgatct ggtcgatcac cttctcgttt
      241 ttcgacccct tggggtagag cgccccatag gtctcgccgg tggcgtactg gccgaccacc
      301 tcgaagacgc cgttggaggt tcccgcctgc gccagcacaa tggccgtatc ggtcatggcc
      361 acgtcgatct ggcctgcctg cagcgcggtg aacatggacg gcgtgtcagg gtagacgctg
      421 gccggcgtct tcggcttcag ttgctggtcg acgaaagcgg cgcccgtggt cgcctgctgc
      481 acgccgatcc gcatgtcccg gatcgagttt ccgtcgacct tggtgccctt cttgaccatg
      541 acgccgatgt cggacgagaa gtagggcacc gaaaaatcga caaccttctt gcgttcggcg
      601 gtaacggaga tctccgacag cgccaggtcg aagtccctgg tctgaccggc gaccagcgca
      661 tcccacgcga cgttgacgac ctcgaccttg tcgaagccgg cacggtgggc aatattggca
      721 gccaggcagt attcatagcc gtccttgatc gtctcgggcg tatcgccgtt ccaccagccg
      781 ggcgccggca ggttgacctc gacggtcaat tgtccggcct ttgccggcgt gaacgggaac
      841 tcgcctttgg tccccgtaag ctcgcaattg ccgatcatgt ccgccgcggc cgcgacgggc
      901 gctccagccg acagcatgac cagggcaagg gcaacgccaa gcacgccggt aaatgttgct
      961 tcagatttca tgacagcctc ccgctgtggt ttgtgattct ttgataaatt ggcccaacgg
     1021 ggccgtgttc gcccccgaag cgcgaccaat agtatcggcc cccaggagcc ataacaaggt
     1081 caccggacgc catgtcgcat gcccctgcga tcacgcagaa ggggcagctg ggcagtgctg
     1141 tcagccgccg aacggctccg ccagcggcat caggcggatg cctttcctga tgcgcttgta
     1201 gggcaggtcg cggttgtcga ccgggttggc gcctggggcg gcgacgatca ggatctcctc
     1261 ggcgatcggc tggaagtcgg cgcggaaatg cacggagctt ttcagcgcca ggatcttctg
     1321 ctccttcggc tcgatgccga cgtggcgggg ggcggttagc cgcttaaccg gcccacgtcc
     1381 gctgtcggac aggtcgcgta ctggcagggc gatagctcac ggcgcagcct atggcagttg
     1441 ccggtaatag tgcctgactg gtgatgtcca actttggagc acgagtgacg cagaacgatc
     1501 ggacaaacgt ttgaggaaaa gtccctggaa ctgagaagcc aatgtacgcc ccgacgtgta
     1561 ctattgtaaa accccgatca tccgcagtcc tcctaggatc atctgtacac cgagtgccac
     1621 ctgcatgaca ctgagcacgg ttccgacgat ctgcaaaggc atcaagccga tgaccttgag
     1681 gatctttcgc gcgtagagca tcgcgagcag gttgaggacc atgacaccta tgagcgccgc
     1741 ggcaacgccg atcacgtagc cggcattcgg actgaccgag agaagaatga tgacggcagc
     1801 gatgccgtat ggcgtcacga tggtggggaa ggcgaggcgg attgccatgc cggcgagcgc
     1861 cggctctggt tggcgcgcct cgatctcgga tgccggtgca tattgctgca gcacttgcct
     1921 cagcgccacg aggaagagga tgaccccgcc ggcgatcaag agcgcgccca gcgaaacatg
     1981 ccagttctcc agtatggcgc ggccaatggc tgcagcaagc agagtggtca cgctcgcaat
     2041 ggcaaaggca cgaatggcga gtttgcgg
//
LOCUS       DASSRL010000492         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_275263, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000492 DASSRL010000000
VERSION     DASSRL010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275263"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="VFL49_09995"
     CDS             <1..1779
                     /locus_tag="VFL49_09995"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET7912352.1"
                     /translation="NVTSESPEKAAKIANAIADSYFYEQVHSKFETQKIAASWFDSQI
                     DELKSKVEASDRAVEKFRAANNLTIAQQGATINDQQLADLNNKLIDAHVQTSEARAKF
                     EQVENIAKTHADPGTLGQALSSGVIGQLRAQYAEVTKTLADVSSKFGPQHPLVVNARA
                     QLRETQKLINEEVQRILENTRQAYRVAQSREDALQKSLDSLTKVSNESGNDQVRLREL
                     QREADANRELYTSFLARYKETRAQESLELPDSRIVSKADIPISPSFPKTTLILALALV
                     AGVSCGSAMAFVADKLDRRIKSQRQAEEITGVPTLAAMPLIGTRELASRAIRGRQELS
                     RYDPDAVRLLPPAMQPPLMRYVLDEPTSLFAESVRAVRLAIHRAARTESVKTVVVTSS
                     IDGEGKTTLAANLAVSLAAVGMRTILVEGDLRNPEMSRSLCPQASVGAIEVAAGRAHF
                     EQALLVDRTTGLAVLPSPPRQSTAGYSEFIFSDAMSNLVEQLRRHFDYVVIDSPPLVP
                     LVDARALAEIADRVVLTIRWDGTPRDVVVQALETLAPAYDRVLGTVLTRVDMRRLRFF
                     DYYQSSSYLKPYSYLGQPRPGRASSS"
     gene            2007..2678
                     /locus_tag="VFL49_10000"
     CDS             2007..2678
                     /locus_tag="VFL49_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7912353.1"
                     /translation="MEFLSHTEWPMWFGYAAVVSSIVTCAMKTMIPLRIVSMTCNSLF
                     IIYGFFGGIYPTLILNLILLPLNTVRLHQMRKLIQDVEAAASMGETSIDWLKPFMSRR
                     SFRKGDIVFRKGDVADAMHYSVSGRYRLSEMGIDIPTGQVFGDLGLLAPGNLRTQTIE
                     CTEDGDVLTATYEQVKELYFQNPQFGFYFLRLTSERLFDNIKRLEQEIVRKNEVIAEL
                     GGKAG"
     gene            complement(3609..3761)
                     /locus_tag="VFL49_10005"
     CDS             complement(3609..3761)
                     /locus_tag="VFL49_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912354.1"
                     /translation="MVHVAVTPHPVVRNMQRNTFVCYTCKRTRTYMLPGTFTPEVEGV
                     AAQRAS"
BASE COUNT          897 a         1106 c         1146 g          935 t
ORIGIN      
        1 aatgtcacct cagaaagtcc ggagaaggct gcaaaaatcg cgaatgcgat cgccgattcg
       61 tatttttatg agcaggtgca cagtaaattc gagacgcaaa aaatcgccgc ctcttggttt
      121 gatagccaga ttgatgaact caagtcgaag gtggaggcat ccgatagggc ggtcgagaag
      181 tttcgtgctg ccaacaattt gacgatcgcg cagcaaggcg ccaccatcaa tgatcagcag
      241 cttgccgacc tgaacaacaa gctcatcgat gcacacgttc aaacctctga ggcccgcgcc
      301 aaattcgaac aggtggagaa tatcgccaag actcacgccg atccgggtac cttgggccag
      361 gcactgtctt cgggcgtcat cgggcagtta cgggcgcaat atgcggaagt aacgaagacc
      421 ctagctgacg tgtcgagtaa attcggcccg cagcacccat tggtggtgaa tgcgagagcc
      481 cagctgcgcg agacacagaa gcttatcaat gaagaagtgc agcgaattct ggaaaacaca
      541 cgtcaagcct atcgggtggc gcagtcgcga gaggacgcct tgcaaaagag tctcgatagt
      601 ctcacgaaag tttcgaatga atccggaaat gatcaagttc gactgcgcga gcttcagcgt
      661 gaggcagatg ccaatcgtga actgtacaca tcgtttctcg ctcgttacaa ggaaacgcgc
      721 gcgcaagaaa gtttggagtt acctgactca cgtatcgtct ccaaagcgga tatccctata
      781 agcccttcgt tcccaaagac gacccttatt ctcgcgctcg cattggtcgc gggcgtgagc
      841 tgtgggagcg cgatggcgtt tgtcgccgac aaactcgacc ggcggatcaa atcgcaacgg
      901 caggccgaag agataaccgg cgttccgacc ctcgccgcga tgccactgat tggcacacgt
      961 gaacttgcca gccgggcaat tcgtgggcgg caggagttga gtcggtacga tccggatgcc
     1021 gtgcggttgc ttccgccggc gatgcagcca ccgctcatgc gatatgttct cgacgagccg
     1081 acatcattgt tcgctgagtc ggttcgcgcc gttcgcctcg ctattcatcg cgcagcgcgc
     1141 accgaatcgg tcaaaaccgt cgtagtgacg tcgtcgatcg acggtgaagg caagacgacg
     1201 ctcgcagcga atctcgcggt ttcgcttgcc gcagtcggca tgcggacgat ccttgtcgag
     1261 ggggacttgc gcaacccgga aatgagccgg tcgctatgcc cgcaggcgag tgttggtgca
     1321 attgaagtcg ccgctggtcg ggcgcatttc gagcaggcgt tgctcgtcga tcggacgacc
     1381 ggtcttgcgg ttttgccgtc gccgccaaga caaagcacag cgggttatag tgaatttatc
     1441 ttttccgacg ctatgagcaa tttggtcgaa cagctgcggc gccacttcga ttacgtcgtt
     1501 atagattcgc cgccgcttgt tccgttggtc gatgctcgcg ccttggctga aatcgcggat
     1561 cgtgtcgttc tcaccatccg ctgggacggc accccgcgcg atgtcgttgt gcaagcgctg
     1621 gaaaccttgg ctcccgccta tgaccgggtg ctgggcactg ttcttacgcg cgtcgacatg
     1681 cggcgtttgc gattcttcga ttactatcaa agttcctcct atctaaaacc gtattcttat
     1741 ctcgggcagc cgcgtccagg gcgtgcgtca tcctcatgag gcggcgcgct ttgcgatcgg
     1801 aatttgtgat ccagaacgcg agcagaacgg cgaacaccta actcttggcg ccgagtcctg
     1861 attcgtcctc tgcattcgcc gcaggcgttg ctacgtctat caagattacc ccacgctata
     1921 taatataccg aagaattcga tccgcctttc gaagtcgacg gcgggatcgc tttatttgag
     1981 acaagattga tcgcggggga aaactaatgg aattcctgag ccataccgaa tggccgatgt
     2041 ggtttggcta tgcggccgtc gtgtcaagta tcgtcacctg cgcgatgaaa acgatgattc
     2101 cccttcgtat tgtcagcatg acctgcaaca gcctcttcat catctacgga ttttttggag
     2161 ggatatatcc cacgctcatc ttgaatctga ttctcctgcc gctcaacacc gtgcgtctgc
     2221 accaaatgcg gaagctgata caggacgtgg aggcggccgc ctccatgggc gaaacgtcga
     2281 tagactggct gaagccattc atgtcgcgcc gcagcttccg caagggcgac atcgtcttcc
     2341 gcaaaggcga cgttgccgat gcgatgcact acagcgtcag cggacgttac cggttaagcg
     2401 agatggggat cgatattccg accggccagg tcttcggcga tctgggtctg cttgccccgg
     2461 gcaacctgcg cacccagaca attgaatgca ctgaggatgg agatgttctg acagcgacct
     2521 atgagcaggt caaagagttg tatttccaga atccgcaatt cggattctac tttctccggc
     2581 tgacaagcga acgactattc gacaatatca aaagactgga acaggagatc gtacggaaga
     2641 atgaggtgat cgcggaactc ggtggaaagg ccggttgagc gactacaatc ttcaatcctg
     2701 gtgcggtgcg tatgagcgtc gagattgctt tatgcaatgg ttccgtcgcg cgatgccgcc
     2761 ccgtggttat gtgcaagtta cgcctgctat gaactgcaaa ccagttgact ttcaacaggt
     2821 gcctttgtca cttaggcagt cgtcgattct aggcgttcgg ccgtgtcaga aaaaagatcc
     2881 gtctatcgca aacgcgtcct taagaccgcc cagatcatcc tcagcgacaa ggcgccgaag
     2941 ctggattgtt ccgtcaggaa tctttcggcc acgggcgcct gccttcaggt ttcgacgacc
     3001 tatggcctcc cattgagctt cgatgtggtg atcgacggcg cgcgacgcca atgccgtgca
     3061 atttggagaa cggacacaaa tattggcatc atgttcatct agcggtgaac cttcgcagga
     3121 ttgcgggccg ccgtgtcaaa gctaaaattc gatacaacta tttatacaat aataaaacag
     3181 tctgcccgac catgtgggcc ttgcgcagga gccgccaatg cgcgattggc tgtttttgct
     3241 ggcaccacca gctctgatct tttacttcat tgcctaccct gacaagttca tagaatttgt
     3301 cttttgggca aaaagactga ttggctgatt gccaagcttt tagctcactc aaataatacg
     3361 ccgatccttt ttccgtccgt ccagacgacg cgacagtgtt tggagccgat ctcgtcgtcg
     3421 atggtcaggc ggaagacgcg cggcagccca aaggtactcg gaacgtgaag gccggcgccg
     3481 gtggctgtca aatcctcgac tgtgcatgaa agctcttgtc cggcgggacc aaatttgatg
     3541 atcccgcgcc gatgggtgga tacgcgggtg tcttgccggc gctcgttcag ggtttgtggg
     3601 tctgccattt acgacgccct ttgcgcggcg acaccctcca cctcgggcgt aaacgtgcct
     3661 gggagcatgt aggtgcgcgt gcgcttgcac gtgtaacaga caaatgtatt gcgctgcatg
     3721 ttgcgcacga ccggatgcgg cgtgaccgca acgtgaacca tttcagtccg gcattgcgga
     3781 cacatggcag tgttgtcttt cggatccagc cccgacatcg gtggtcgcgc cttaaaacct
     3841 gcccaagaag ggcaggggtt caattcgaac gatgccgttc gaaaaatagg tcacgaagtt
     3901 taagaattcc cttcctggtt gaatttcttg gtttttctgt cgccgtttta cagtgttatc
     3961 agcgttcatc gaccgttcgt cggataaaat gccggccggt catgtcgatt cgtcccgtga
     4021 tggggtgcga gaatgcgacg atcacgggtt aaagcaattt gtccgttacg cacgccttaa
     4081 gcgc
//
LOCUS       DASSRL010000493         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_380714, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000493 DASSRL010000000
VERSION     DASSRL010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380714"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1912)
                     /gene="araD"
                     /locus_tag="VFL49_10010"
     CDS             complement(185..1912)
                     /gene="araD"
                     /locus_tag="VFL49_10010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HET7912355.1"
                     /translation="MNARKRPEDLRSHRWLGVNDLRAFGHRSRLRQGGFDAADFRGKP
                     VIGIVNTWSDINFCHTHLRDRAEDVKRGVLQAGGFPVELPAMSLSEPFVKPSTMLYRN
                     LLAMETEELLRSHPIDGAVLMGGCDKTTPALIMGAVSMGLPAIYLPAGPQLRGNWRGQ
                     ELGSGSDAWKYWDEKRAGRISEADWAELEGGIARSFGTCMVMGTAATMMAIAEALGLS
                     LPGASSIPAADAAHPRMCAAAGRRIVEMVWEELTPEKILTSSAFDNAIRVHMAMGGST
                     NAIIHVVAMARRAGLKLDMKRFDEISREIPVLANVRPSGKFLMEDFFYAGGLRALMGE
                     LKSALDLSCLTVTGRSIGENIAGAEVYKRDVIHPLGDPVSRDGATAVLTGNLAPRGCV
                     MKPSAAEKRLLRHRGRVIAFEDYNHMACEVERDDLDVTPDHILVLKNGGPKGGPGMPE
                     WGMLPIPKKLVKQGVRDMLRISDARMSGTSYGACILHVAPESFIGGPLAFVQTGDEIE
                     VDVPARRIHLHVTDEELARRRAGWAQPAPRYPRGYGALYSEHIGQADEGCDFDFLAPL
                     GAIPEPEIH"
     gene            complement(1909..>2219)
                     /locus_tag="VFL49_10015"
     CDS             complement(1909..>2219)
                     /locus_tag="VFL49_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HET7912356.1"
                     /translation="IVPFMFVYAPELLMIGEWPAIIWASLSATIGVLLFSAGLHGYFI
                     THSAHWQSVLLVVAGLLLIDPHFSTDVIGAVLAAVVVAAQFAARRAHTKVPKAEIAVE
                     "
BASE COUNT          433 a          715 c          696 g          375 t
ORIGIN      
        1 aggcgaacag ccggcatgat ttcctcatac gccggagtca agatccggca atcaggtcgc
       61 gtgacataag tcgcccagcg gcagacgcaa ttgaattttc acgcatgagt caatgcacgg
      121 agcaaacatt agaacgcttc catttaggag acgttctaat ctgtcaaacc ggcaagacgc
      181 tcgatcaatg aatctcaggc tcgggaatcg cgccgagcgg tgcgaggaaa tcgaaatcgc
      241 agccctcgtc ggcctgaccg atatgctccg aataaagcgc gccatagccg cgcggatagc
      301 gcggcgcagg ctgcgcccag ccggcgcggc ggcgggcaag ctcctcgtcc gttacatgca
      361 gatggatgcg gcgtgccggc acatcgacct cgatctcgtc gcccgtctgc acgaaggcga
      421 gcggcccgcc gataaagctt tccggcgcaa cgtgcaaaat gcaggcgccg tagctggtgc
      481 cgctcatgcg cgcatcggaa atacgcagca tgtcgcgaac gccctgcttc accagctttt
      541 tcgggatcgg caacatgccc cactccggca tgccgggccc gcccttgggc ccaccattct
      601 tgagcacaag gatgtggtcg ggagtcacgt cgagatcgtc gcgctcgacc tcgcatgcca
      661 tgtggttgta gtcctcgaag gcaatcaccc tgccgcggtg cctgagcaag cgcttttccg
      721 cggcggacgg tttcatcacg cagccgcgcg gtgccagatt gccggtgagc acggcggtgg
      781 cgccgtcgcg cgataccgga tcgccgagcg gatggatgac gtcgcgcttg tagacctcgg
      841 cgccggcaat gttttcgccg atgctcctgc cggtcaccgt caggcatgac agatcgagcg
      901 cgctcttcag ctcgcccatc agagcgcgta atccgccggc gtagaagaaa tcctccatca
      961 ggaatttgcc ggacgggcgc acgttggcaa gcaccggaat ctcgcgcgag atctcgtcga
     1021 aacgcttcat gtcgagcttg aggccggcgc ggcgcgccat cgccacgacg tgaatgatgg
     1081 cattggtcga gccgcccatc gccatgtgca cgcggatggc attgtcgaag gccgacgacg
     1141 tcaggatctt ttccggtgtc agctcttccc acaccatctc gacgatgcgg cggccggcgg
     1201 cggcgcacat gcgcggatgc gccgcgtccg cggccggaat cgaggacgcg cccggcagag
     1261 aaagtcccag cgcctcggcg atcgccatca tagttgccgc cgtgcccatc accatgcagg
     1321 tgccgaagga gcgcgcgatg ccgccctcga gttcggccca gtcggcctcg ctgatgcgac
     1381 cggcgcgctt ctcgtcccag tatttccagg catcggatcc cgagccgagc tcctggccac
     1441 gccagttgcc gcgcagctgt gggccggcag gcaggtaaat cgcgggcagg cccatgctga
     1501 cggcgcccat gatcagcgcc ggcgtggtct tgtcacagcc gcccatgagc acggcgccgt
     1561 cgatcggatg gctgcgcagc agctcttccg tctccatcgc cagcagattg cggtagagca
     1621 tcgtcgatgg tttgacgaaa ggttcggaca gcgacatcgc cggcaattcg accggaaagc
     1681 ctccggcctg cagcacgccg cgcttcacgt cttccgcgcg gtcacgcaga tgcgtgtggc
     1741 agaagttgat gtcgctccag gtgttgacga tgccgatcac cggcttgccg cggaaatcgg
     1801 cggcatcgaa gccgccctgc cgcagccgcg agcgatgccc gaaggcgcgc aggtcgttga
     1861 cgccgagcca gcgatggctg cgcaggtctt cgggacgttt acgtgcgttc attccaccgc
     1921 gatctcagcc ttgggaacct tggtatgtgc gcgccgggcc gcaaactgag cggcgacaac
     1981 gacggcggcg agcacggccc cgatcacatc cgtagagaaa tgcggatcaa tcagcaatag
     2041 gccagcaaca accaaaagca cgctttgcca gtgcgcggag tgagtgatga aatatccatg
     2101 cagcccggcc gaaaacagca acacgccgat ggtggccgac aggctcgccc agatgatggc
     2161 gggccattct ccgatcatca ggagttcggg cgcataaaca aacatgaacg gcacgatga
//
LOCUS       DASSRL010000494         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_677744, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000494 DASSRL010000000
VERSION     DASSRL010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677744"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFL49_10020"
     CDS             <1..257
                     /locus_tag="VFL49_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7912357.1"
                     /translation="VPASYELGYKVALPQFRSIVVRAGTPPDIVKKLSDALHKVYESP
                     EYKKFLEDQFADPNSFEDASKAPAFVNEQLNDMKKTMATN"
     gene            285..824
                     /locus_tag="VFL49_10025"
     CDS             285..824
                     /locus_tag="VFL49_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET7912358.1"
                     /translation="MDKALFRSVLPYAIGLAIALALYVYAGTIVYSQRPGELGPETWP
                     RLAIQLMGGSCLFELSRRLIVGNKDATGFLEAFDREEAEEKQPVYPRLLIGGIVLMAI
                     YAVLVPVLGFILGTFLFLAAFMYVGGYRAHAAIWGTTTVVTVFCGILFLRIAYVSLPR
                     GVPPFDRLTDVFFAIPSLW"
     gene            829..2331
                     /locus_tag="VFL49_10030"
     CDS             829..2331
                     /locus_tag="VFL49_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET7912359.1"
                     /translation="MIDILVQLAHGFVHCLTPLNMAMLLIGIVIGLLIGVLPGLTLVM
                     GVALALPFTYKMDVTASIVLLTAMYVSGTYGGAFTAILFRIPGEPIDVPMLWDGYTMG
                     RKGRPAKALGWTLVAALGGGLLSAVVMVALTEPLAKFALRFSSPEYFVIVMFGLLSVV
                     AIGKGSLSNAFISMSVGILISTVGTDPIYGAYRFTFGSPILADGIEFLVVMVGAYGVG
                     EVLTRLETGFATKPLDKISNARTELPTLKELNDIKGMFFRSSVVGDLIGLLPGAGATI
                     ASFVSYGIEARFGRRKNQMGTGVAEGIVAPQAAATASVGGAMVHLLALGIPGSGATAV
                     ILGAFMLHGIQPGPQVLVSSAPLVYTVFASLFLGLALMCAIGYFAIRPLVKILDFPEA
                     IVSAYVLILCFIGALSIRNNITDLWLIIGFGILGYGFERLKFPVAPLVLGVILGPIAE
                     EAFMNTMISFSNDWTVFFTRPISGTIVALTIIVLLLPFAQRLIRSRLVTA"
     gene            2350..>2592
                     /locus_tag="VFL49_10035"
     CDS             2350..>2592
                     /locus_tag="VFL49_10035"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7912360.1"
                     /translation="MRTNLGLDERTTTAPAPRRTVTGLNVPAPRAKGHAQKLGATARS
                     FAILEHVAGSPTPVDVFDIIAKLKLPKATAYRLVDWF"
BASE COUNT          403 a          814 c          803 g          572 t
ORIGIN      
        1 acgtgcctgc ttcctacgag ctcggctaca aggtggcgct gccgcagttc cgctcgattg
       61 tcgtgcgcgc cgggaccccg cccgacatcg tgaagaagct ctcggatgcg ctgcacaagg
      121 tctacgagtc gcccgaatac aagaaatttc ttgaagacca gtttgccgat cccaacagct
      181 tcgaggatgc ctcgaaggcg ccggccttcg tcaacgagca gctcaatgac atgaagaaaa
      241 cgatggcgac gaattagtac gcgggtgcga gcggagtagg ggtgatggac aaggcgctgt
      301 tccgctcggt cctgccatac gcgatcggtc tcgccatcgc gctcgcgctc tatgtctatg
      361 ccggcaccat cgtctactcg cagcggccgg gcgagctcgg gcctgagacc tggccgcggc
      421 tcgccatcca actgatgggc ggctcctgcc tgttcgagct gtcgcggcgc ctgattgtcg
      481 gaaacaagga cgcaaccggc tttcttgagg ccttcgaccg cgaggaggcg gaagaaaagc
      541 agccggtcta tccgcggctt ctgatcggcg gcatcgtgct gatggcgatc tatgccgtgc
      601 tggtgccggt gctcggcttc atccttggca cgttcctgtt tctggccgcg tttatgtatg
      661 tcggcggcta tcgtgcccat gccgcgatct ggggcacgac gacggtggtg accgtctttt
      721 gcggcatcct gttcctgcgc atcgcctacg tgtcgctgcc gcgtggcgta ccgcctttcg
      781 atcgtttgac cgacgtcttc tttgcgatcc cgagcctgtg gtgacgccat gatcgacatc
      841 ctggtgcagc tcgcccacgg tttcgtgcac tgcctgacgc cgctcaacat ggcgatgctg
      901 ctcatcggca tcgtcatcgg gctgctgatc ggcgtgctgc cgggattgac gctggtgatg
      961 ggggtcgcgc tggcgctgcc cttcacctac aaaatggacg tgacggcatc gatcgtgctg
     1021 ctcacggcga tgtacgtgtc cggtacctat ggcggcgcct tcacggcaat cctgtttcgt
     1081 attcccggcg agccgatcga tgtccccatg ctgtgggacg gctataccat gggccgcaag
     1141 ggaaggccgg ccaaggccct tggctggacg ctggtcgccg cgctcggcgg cggcctgttg
     1201 tcggcggtcg tcatggtcgc actcaccgag ccgctggcga aattcgcgtt gcggttttcc
     1261 tcgcccgagt atttcgtgat cgtgatgttc ggtctgctga gcgttgtcgc catcggcaag
     1321 ggttcgctgt ccaacgcctt catcagcatg agcgtcggaa tattgatctc gacggtcggc
     1381 accgacccga tctacggcgc ctatcgcttc accttcggct cgccgatttt ggccgacggc
     1441 atcgagtttc tcgtcgtcat ggtcggcgct tacggcgtcg gtgaggtgct tacccggctg
     1501 gaaaccggtt ttgcgaccaa gccgctcgac aagatctcca atgcgcgtac cgagctgccg
     1561 acactcaagg aactcaacga catcaagggc atgttcttcc gctcctcggt ggtcggcgat
     1621 ctcatcgggc tgttgccggg ggcgggcgcg acgatcgcct cgttcgtcag ctacggcatc
     1681 gaggcacgct tcggccgccg gaagaatcag atgggcaccg gcgttgcgga aggcatcgtt
     1741 gcgccgcagg cggcggcgac cgcttcggtc ggcggtgcga tggttcacct gcttgcgctc
     1801 ggcattcccg gtagcggcgc gaccgcggtg attctcggcg ccttcatgct gcacggtatc
     1861 cagccggggc cgcaggtgct ggtgagctcg gcgccgctcg tttacaccgt gtttgcttcg
     1921 ctttttctcg gcctcgcgct gatgtgcgcg atcggctatt tcgccatccg gccgctggtg
     1981 aagatcctcg atttccccga ggcgatcgtg tcggcctacg tgctgatcct ctgcttcatc
     2041 ggcgccctct cgatccgcaa caacatcacc gatctgtggc tgatcatcgg attcggcata
     2101 ttgggttacg gctttgaaag gctgaaattt cccgtcgcgc cgctcgtgct cggcgtcatt
     2161 ctcggaccga tcgcggaaga agccttcatg aacacgatga tcagtttctc gaacgactgg
     2221 accgtatttt tcacgcgtcc catctccggg accatcgtcg ctttgaccat catcgtcctg
     2281 ttgctgccgt ttgcgcagcg tctcatcaga agccggcttg tgacggcttg aaccgaggtt
     2341 aggtgaccca tgcgtaccaa tcttggactt gacgaaagga ccacgacagc gcccgcgccg
     2401 cgccgtaccg tgaccgggct caacgtgccc gcgccgcggg ccaaaggcca cgcgcagaag
     2461 ctgggcgcta ccgcgcgttc ctttgccatt ctcgaacatg tggccggctc gcccacgccg
     2521 gtcgacgtgt tcgatatcat agccaagctg aaattgccga aggcgacggc ctaccggctg
     2581 gtcgactggt tc
//
LOCUS       DASSRL010000495         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1745527, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000495 DASSRL010000000
VERSION     DASSRL010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745527"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFL49_10040"
     CDS             complement(<1..279)
                     /locus_tag="VFL49_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET7912361.1"
                     /translation="MKTPAVLLALLLALPLAQATPGRAAESQIAVVAAENFYGDIARR
                     IGGDRVSVVSIMNNPDQDPHLFETTPGIVRQIAAAQIVILNGANYDSWM"
     gene            complement(376..1536)
                     /locus_tag="VFL49_10045"
     CDS             complement(376..1536)
                     /locus_tag="VFL49_10045"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET7912362.1"
                     /translation="MRLGATQAISGRRQEEILPKTRMSQAASVIEELEDVLAAGSSDR
                     RSEILRRVTDLFLGNAESFSHDQVGVFDDVLAHLIHQVETKALAELGARLAPVGNAPN
                     GVIRTLARHDEISVAGPVLAQSPQLTDGDLVEIAGLKGQGHLGAISERKRLAAAVTDI
                     LIQRGDTTVVRKLSQNQGATFSENGYETLATRAETDEQLAENLGVRLDMPPQLLQSLM
                     SKATETVRARLRAVVPLEDQAAIQQVLASVSDKVMRKAAAPRDFGRAVAVIDRMQEQG
                     RLNEIAIAGFAGEGKYEEMVAGLARICVAPTELVERLMQNPRYDGVLVACKAAEFRWP
                     TFSAILKARFTSYEMSTAELTQARADFIKLSVTTARRMFRFWLVRGVAKANS"
     gene            complement(1683..>2659)
                     /locus_tag="VFL49_10050"
     CDS             complement(1683..>2659)
                     /locus_tag="VFL49_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912363.1"
                     /translation="ETMPKLLSRLLAASLLAVALVPGALADDYPNRPVRLIVPFPPGG
                     SNDVVGRLVAQQLSNQLGQQVFVDNRGGAGGVIGTEACATAAPDGYTLCIISIAHAVN
                     PALHKLNYDPIKSFTPISIFATGPNVLAINPTLPVNSVKELVALAKQKPGELNYASAG
                     VGSFQHLGGELFKLTAGVNIVHVPYKGGGPAMQDVISGHVKIMFSSLIQTTPFIKSGQ
                     LKALGTGGAKRTSVLPDVPTISEAGVPGYVADNWWGLAAPAGLSAALLDRLYKASQAS
                     LQAPELRAQFEREGAATVTMTTAEFADYIKSEIAKWGRVVKEGKISAQ"
BASE COUNT          496 a          810 c          825 g          528 t
ORIGIN      
        1 catccaggaa tcatagttgg cgccattgag gatcacgatc tgggccgccg cgatctggcg
       61 cacgatgccg ggcgtggtct cgaacaagtg cggatcctga tcgggattgt tcatgatgct
      121 gacgaccgag acccggtcgc cgccgatccg gcgggcgatg tcaccgtaga aattttccgc
      181 ggccacgacg gcaatttggc tttccgcagc gcgaccgggt gtggcttgcg cgagaggtaa
      241 tgccagaagc agggcgagca gaaccgctgg ggttttcatc ggcaaatccg tctcagacga
      301 aaaatgtaac attataacat ttcacccgca agccggggtc ctgtgtcgcg gccttcaagc
      361 ttcaagtatg agttgtcagg agttggcttt cgccacgccg cgaacgagcc agaagcggaa
      421 catccgccgc gccgttgtca ccgaaagctt gatgaagtcg gcgcgagcct gcgtcaattc
      481 ggcagtcgac atttcgtagg acgtaaagcg tgccttgagg attgcgctga atgtcggcca
      541 tcggaactcg gcagccttgc aagcgaccag aacgccgtcg tagcgcggat tctgcatgag
      601 tctctcgacg agttcggttg gcgcgacgca gatgcgcgcc agtcccgcga ccatttcctc
      661 gtacttgccc tcgccggcaa agccagcaat ggctatttcg ttgagacggc cttgctcctg
      721 cattctgtcg atcaccgcga ccgcgcggcc gaagtctctt ggcgcggcgg ccttgcgcat
      781 gactttgtct gaaaccgagg ccagcacctg ctggatcgca gcctgatcct cgagagggac
      841 gactgcccgc aagcgtgcgc gaacggtttc ggtcgccttc gacatcaggc tctgcagcag
      901 ctgcggcggc atgtccaggc gaacgccgag attttccgcg agttgctcgt cggtctcggc
      961 acgcgttgca agtgtctcgt agccgttttc agagaatgtt gcgccttggt tctgcgaaag
     1021 tttgcggacg actgtcgtat cgccgcgctg gatcaagata tcggtcactg ctgccgcgag
     1081 acgcttgcgt tccgagatcg cgcccaggtg gccctgacct ttcaatccgg cgatctccac
     1141 aaggtcgcca tcagtcaact gcggcgactg cgcaagaacc gggccggcga cgctgatctc
     1201 gtcgtggcgc gcgagggtgc gaatgacgcc gttgggtgcg ttgccgaccg gcgccagcct
     1261 tgcgccaagc tcggcaagcg ctttggtctc aacctgatgg ataaggtggg cgagcacgtc
     1321 gtcaaacacg ccgacttggt cgtgactgaa ggattcggcg ttgccgagaa agaggtcggt
     1381 tactcgcctg aggatctcgc tgcgacgatc gctcgagccc gcggccagaa catcctcgag
     1441 ctcttcaatg acggaggcag cctgcgacat tcttgtcttg ggcaggattt cttcctgccg
     1501 ccttccactg atcgcctggg tcgcccccaa acgcacgcct gcaccttatc ggcggcccgt
     1561 aaagctaagg taaaactggc gcccctcgaa tcggcaaaac cgacagcggt ctgccgggcc
     1621 tggcgccgtc ctgcggcagg accaaccggc ccgtctcgca cggttaagcg caccttaatg
     1681 cgctactgcg cgctgatttt gccttctttg acgacccggc cccatttcgc gatttctgat
     1741 ttgatgtagt cggcgaattc ggccgtcgtc atggtcaccg ttgccgcgcc ctcccgctcg
     1801 aactgcgcgc gcaattcagg ggcctgcagc gatgcctggc tcgctttgta cagccggtcg
     1861 agcaaagcag cggacagtcc ggcaggtgcg gcaaggcccc accagttgtc ggcgacatag
     1921 ccgggcacgc ccgcttcgga gattgtcggc acatcgggca gcaccgaggt gcgcttcgcg
     1981 cctccggtgc cgagcgcctt aagttgaccg gatttgatga acggcgtggt ctggatcagc
     2041 gaagagaaca tgatcttcac atggccgctg atcacgtcct gcatggccgg accgccgcct
     2101 ttgtacggca cgtgcacgat gttgacgccg gcggtgagtt tgaacaattc gccgccgagg
     2161 tgctgaaagc tcccgacccc ggcggaagcg tagttgagtt cgccgggctt ctgtttggcg
     2221 agcgcgacca gctccttgac ggaattcacc gggagcgtcg ggttgatcgc gagcacattg
     2281 gggccggtcg caaaaattga gatcggcgta aatgatttga tcggatcgta attgagcttg
     2341 tgcaacgccg gattgacggc atgcgcaatc gaaataatgc aaagggtgta gccgtccggc
     2401 gcagcggttg cacacgcctc cgtaccgatc acgccaccgg cgccgccgcg attatcgaca
     2461 aaaacctgct ggccaagctg gttactgagt tgctgcgcga cgagccgccc taccacgtcg
     2521 ttgctgccgc cgggtggaaa cggaacgatc aaccgcaccg ggcggttcgg gtagtcgtcg
     2581 gcaagcgcgc ccggaacaag cgccactgca agcaaactcg ccgcaagcag tcgtgacaga
     2641 agtttcggca ttgtctccc
//
LOCUS       DASSRL010000496         5521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1623073, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000496 DASSRL010000000
VERSION     DASSRL010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623073"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VFL49_10055"
     CDS             complement(<1..137)
                     /locus_tag="VFL49_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol degradation protein meta"
                     /protein_id="HET7912364.1"
                     /translation="MRFGVVAAALLSLCFSNGARADEGGISFWVPGFFGSLAATPQQP
                     G"
     gene            complement(264..689)
                     /locus_tag="VFL49_10060"
     CDS             complement(264..689)
                     /locus_tag="VFL49_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7912365.1"
                     /translation="MSKAPIRVAIVDDEASVRTALARLLAASSFEPRTYGSAREFIDS
                     IRIDVPECLVVDVHMPDFTGLDLQRYLNRINKRIPTVVITAFDDAGVRERSTASGAKA
                     FLTKPLHGPTLIEAILSATGRALPGASQARAANLRKRRS"
     gene            complement(753..1385)
                     /locus_tag="VFL49_10065"
     CDS             complement(753..1385)
                     /locus_tag="VFL49_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7912366.1"
                     /translation="MGDAGFTTYLVDDDPAVLRSITRLLKAGGYRTRSFSSPQKFLSA
                     HDPSIPGCAIIDLVMSELDGLRLQEALAKSGSQRPIIFLTGRGDVSTSVKAMKAGAVD
                     FLTKPVKRDALFSAVVHAAELDAATRQKRQESKSIDNRLAALTRREREVLEHVIAGRL
                     NKQIAASLGTVEKTVKVHRGRMMAKLGVRSVAELVRLADRAGIAPVRKES"
     gene            complement(1418..3481)
                     /locus_tag="VFL49_10070"
     CDS             complement(1418..3481)
                     /locus_tag="VFL49_10070"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET7912367.1"
                     /translation="MDQIAPDISLRSLEERGARLGDSNTIRNVFIVAIAYYLGAEIAF
                     LIGTLSDKIFAPFWPPNIVLYCALLITPPRQWWIYILATLPAHTIAELGVGMPASQLL
                     VAFVTNCAVAIVNAFAVQRLLVEPPWFGTIAKTIIYVMITGFLSPAFSALGGAFVQIL
                     GGGSLADYGLFWARWYASNALGSLTLGPIAMIYLSGKESSPIRPNRPVLEAALIAITL
                     VAACLVAFEDMPSIASGYLPLLLYLPLPIIVWAAMRFGAKGASSAILLLSLVLIWRTL
                     NGPSLFDIGSPETNVFALQLFLIALSPPILLLGAAVEETRRAERATREREELISFAAA
                     ASHVGLWQHDLSTGTFWATDYCRTLFGLPRSANLDLDALLGRVHPNDSITATQALRSA
                     ISRAVPVDIEFRTLGDADTRWIAARARPVRGADGKPVAMSGAFSDVTSRKIAEAEADV
                     QRQEIAHLMRVSMLGELSGGLAHELTQPLTAILSNAQAGKMLLAKGSKSLSEIGNILD
                     DIISEDERAGEVIHRLRGLLKKGEVKYETINMNDLISSTLQLLHSELIDRRMSVTLDN
                     AADLPATRGDPVQLQQTLLNLLMNAMDATENVPPARRNISVSTAATDAGGVEVRIHDR
                     GTGLPPEQEHEVFQPFFTTKKRGLGLGLSICTSIVKLHGGTLSLQNNVREGVTAVFRL
                     PPPLD"
     gene            3883..4800
                     /locus_tag="VFL49_10075"
     CDS             3883..4800
                     /locus_tag="VFL49_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET7912368.1"
                     /translation="MIRIPGGTFRMGSDRHYPEEAPVHRVTVGDFWIDRAPVTNRQFR
                     EFVRATGHVTFAEIPPDPKDYPGALPNMLFAGSLVFTPPKHPVDLRDWSQWWTFLKGA
                     NWQRPYGPDSNIKGLDDHPVVHIAYSDAFLYARWAGKELPTEAEWEFASRGGLDGAEF
                     AWGGEFMPGNRAMANTWHGQFPHQRKPGFKRTSPVTAFPPNGYGLYDMIGNVWEWTGD
                     WFSARHQADAPKACCIPENPRGAPESDSYDACQPNIRIPRKVLKGGSHLCAPSYCRRY
                     RPAARHAQPVDTSTSHVGFRCVIRRRGES"
     gene            5003..5308
                     /locus_tag="VFL49_10080"
     CDS             5003..5308
                     /locus_tag="VFL49_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912369.1"
                     /translation="MSSTLINLIIQLIAGAIGGNAAGNALKDYDLGTFGNSISGAIGG
                     VAGGTVLQSLIPMLAGAGGGLDVGALLGQAVGGGVAGAILTFIVGLVKNMMAGQPAR"
BASE COUNT         1064 a         1673 c         1753 g         1031 t
ORIGIN      
        1 aatcccggct gttgcggcgt tgccgcaagg cttccaaaaa agcccggtac ccagaagctg
       61 atgccgcctt cgtccgctcg cgccccgttg gaaaagcaga ggctcaaaag cgctgccgca
      121 accacaccga agcgcaaatg cgtttgcaaa taggctgata gattcgctgc cagaccgtcc
      181 tcagaagcga tccacctcat agtccgtccc ccttacctgc tccgcccgca tcgtaacacg
      241 agcggcccat cacgcacgaa cgactaagat ctccttttcc tcaaattcgc cgcccgcgcc
      301 tgcgacgcac ccggcaacgc ccttccggtt gcgctcagga tcgcctcgat cagggtcgga
      361 ccatgcagcg gcttggtgag gaatgccttg gcgcctgagg ccgtgctgcg ctcccgcacc
      421 ccggcatcgt cgaaggcggt aataacgacc gtcggtatcc tcttattgat gcggttgaga
      481 tagcgctgca ggtcaagccc ggtgaagtcc ggcatatgca cgtcgacgac caggcattcg
      541 ggaacatcga tgcggatgga atcgatgaac tcgcgcgccg agccgtaggt gcgcggctca
      601 aatgacgatg cggcgagcag acgggcgagc gcggtgcgca ccgacgcctc gtcgtccacg
      661 atcgcgaccc gtatgggggc ttttgacatg ccgcggcatt gtcgtgcgaa aacgcttgcg
      721 gcgtcgagtt gacccaaggt cctatgttga tactacgatt ctttgcgcac cggcgcgatt
      781 cccgcccggt cggcaagccg aaccagctcg gcgacgcttc gcacgcccag tttcgccatc
      841 atgcgcccgc ggtgcacctt gactgtcttt tcgacggtgc caaggcttgc ggcgatctgt
      901 ttgttgagcc ggccggcgat cacgtgctcg agcacctcgc gctcgcggcg cgtgagcgca
      961 gccaggcgat tgtcgatcga tttgctttcc tgccgcttct ggcgtgtggc cgcgtcgagt
     1021 tcggcagcgt gcacgactgc ggaaaaaagc gcgtcccgct tcaccggttt ggtgagaaaa
     1081 tcgacagccc cggctttcat tgctttcacg ctggtcgaga cgtcgccgcg tccggtcaaa
     1141 aaaatgatcg ggcgttggga gccggatttg gccaaagctt cctgcagacg caggccatcg
     1201 agttccgaca tcacgaggtc gatgatcgcg cagcccggaa tggacggatc gtgcgcggaa
     1261 agaaatttct gcggggacga gaaggacctg gtcctgtagc cgccggcttt caaaagccgc
     1321 gtgatcgacc gcagcacggc cggatcgtcg tcgacgaggt aggttgtgaa tcccgcgtcg
     1381 cccatcacgc atacacctct tggtggcaaa ggttacctta atccagcggc ggtgggaggc
     1441 gaaagaccgc agttacgcct tctctcacat tgttttgaag cgagagcgtg ccgccgtgca
     1501 gcttgacgat cgacgtgcag atcgacaggc cgagaccgag gcctcgcttc tttgtcgtga
     1561 aaaacggctg aaacacttcg tgctcctgct ccggaggcaa tccggtacca cggtcgtgga
     1621 tgcgcacctc gaccccgcct gcgtcggtcg ccgccgtgct gaccgaaatg ttgcgccggg
     1681 ccggcggcac gttttccgtg gcatccatcg cattcatcag gaggttgagc agggtctgct
     1741 gcaattgcac ggggtcgccg cgtgtcgccg gcaggtctgc ggcattgtcc agggtcaccg
     1801 acatccgtcg atcgatcagc tcgctgtgca gaagctgcaa tgtcgaggaa atcagatcgt
     1861 tcatgttgat cgtttcgtat ttgacttcac cctttttcag caagccgcgg agacgatgaa
     1921 tcacttcgcc tgcgcgctca tcttccgaga ttatatcgtc aagtatgttg ccgatttctg
     1981 acagggattt gctgcctttt gcgagcagca tctttcccgc ctgggcgttt gagaggatcg
     2041 ccgtcagcgg ctgcgtcagt tcgtgcgcca ggccgccgga aagctcgccc agcatcgaga
     2101 cgcgcatcaa atgcgcgatt tcctgccgct gaacgtccgc ctcggcttcc gctatcttgc
     2161 gggatgtcac atccgaaaat gctcccgaca tcgccaccgg tttgccgtca gcaccgcgga
     2221 cgggccgcgc ccgggccgca atccaccggg tgtcggcatc gcccagcgtg cgaaattcga
     2281 tgtcgaccgg tacggcgcgt gaaattgccg agcgcagcgc ctgggtcgct gtaatgctgt
     2341 cgttcggatg gacgcggccg aggagcgcgt ccagatccag attggcactg cgcggaagcc
     2401 cgaacagggt gcggcagtaa tcggtcgccc agaatgttcc ggtcgacaag tcgtgttgcc
     2461 acaatccgac atgggaggcc gccgccgcga atgaaatgag ttcttcgcgc tcccgtgtgg
     2521 ctcgttcggc gcggcgtgtt tcttcaacgg cggcgccgag cagcaggatc ggcggcgaca
     2581 gcgcgatcag gaaaagctgc agggcgaaca cgttcgtttc cggcgagcca atatcgaaaa
     2641 ggctcgggcc gttgagcgtg cgccagatca gcacaaggct gaggaggagg atggcgctgc
     2701 tcgcgccttt ggcgccgaac cgcatggctg cccatacgat gatcggcagc ggcagataca
     2761 ggagcagcgg aagatagccg ctggcgatgc tcggcatatc ttcgaatgca acaagacacg
     2821 cggcgacaag cgttatcgcg atgagcgcgg cctccaggac aggccggttg ggtctgatgg
     2881 gtgatgactc ctttccgctc agatagatca tggcgatcgg cccgagcgtg aggctgccca
     2941 gggcgttcga cgcgtaccag cgcgcccaga acaatccgta gtctgccaat gagccgccgc
     3001 cgagaatctg cacgaaggct ccgccgagcg cgctgaacgc cgggctgagg aagccggtga
     3061 tcatgacgta gatgatcgtc tttgcgatcg tgccgaacca tggaggctcg accagcaggc
     3121 gctggacggc aaacgcattg acgatcgcca cggcgcagtt ggtgacgaag gcaacgagca
     3181 attgcgacgc cggcataccg acgccaagct cggcgatcgt gtgcgcgggg agggtggcaa
     3241 gaatatagat ccaccactgc cgcggcggcg tgatcagaag cgcacaatac agaacgatgt
     3301 tcggcggcca gaacggcgcg aagattttgt cggacagcgt gccgatcaga aaagcgattt
     3361 cagcgccgag atagtaagcg atcgcgacga tgaagacatt tcggatcgtg ttcgagtcgc
     3421 caaggcgcgc gccgcgctcc tcgagtgatc gcaatgaaat gtcaggcgcg atttggtcca
     3481 tcgtcgtccg tccgtcggcg tggaccgcta tctatagcat ccgcctttcg ctcgagggag
     3541 gccgttgcaa ttttacgaaa tgtatcgcag cgcgagcata ttggaccacg gtccaatcga
     3601 caggtgcatt ttttgggcag aaactccaaa actaagacat ccattgggat gtcggcctca
     3661 tgatgcgcga ggcggcaatc cccccacctc cccgtccccc tgtcgcctcg cgcgttttgg
     3721 cctctccccc gccgggagag gtcattggac cagggtccaa ctcgattgtg gcgcgcgcga
     3781 gcgtagcatt ggggcatgag cgagcatgcc tggacggaaa ccgagccgcg ccttcgcggc
     3841 gaggctgatg tggccgaagc gccaatcgtc aacaccgccg gcatgatccg gattccgggc
     3901 ggcacgttcc gcatgggatc cgacaggcat tatcccgaag aagcgccggt gcatcgcgtc
     3961 accgtcggcg atttctggat cgatcgcgcg ccggtcacca atcggcaatt cagggaattc
     4021 gtgcgggcca ccgggcacgt gacctttgcg gagattccgc ccgatccgaa ggactatccg
     4081 ggagcgctgc cgaacatgtt gttcgcaggg tcgctggtgt tcacgccgcc gaagcatccg
     4141 gtcgacctgc gcgactggag ccagtggtgg actttcctta aaggcgcgaa ctggcaacgc
     4201 ccttacggcc cggacagcaa catcaagggg ctcgacgatc atccggtggt gcacatcgca
     4261 tactcggatg catttctgta cgcgcgctgg gcggggaagg aattgccgac ggaagctgaa
     4321 tgggaattcg cgtcgcgcgg cggtctcgac ggcgcggagt tcgcctgggg cggcgaattt
     4381 atgcccggca atcgtgcgat ggcgaacacc tggcacggcc aatttccgca ccagcgcaag
     4441 cccggcttca aacgcacatc gccggtgacg gcgtttccgc cgaatggtta tggcctctac
     4501 gacatgatcg gcaatgtctg ggagtggacc ggagactggt tctccgccag gcatcaggcc
     4561 gacgcgccga aggcctgctg cattccggaa aatccgcgcg gcgctccgga gagcgacagc
     4621 tacgatgcct gccagccgaa catcaggatt ccgcgcaaag tgctcaaagg cggctcgcat
     4681 ctctgcgcgc cgagctattg ccggcgctat cggccggctg cgcggcatgc gcaaccggtc
     4741 gatacgtcaa ccagtcacgt tggatttcga tgtgtcattc gaagaagagg cgagtcgtga
     4801 aacgttttct gctgttcagc atgcgcctct acgcgccgag gtctgaggcg ctgacgggcc
     4861 gctggaatcc gccgccggtg ccggcgcgcg agatcaccac cggcgcaaaa tgacgcatag
     4921 ccggtcgaca catcgaccga tcgaacgcgg ggatcagcga ggagggaacg tcaataccag
     4981 agccgtcaaa atggaggcgc tcatgtcaag cacactgatc aatctcatca ttcagctgat
     5041 cgcgggcgcg atcggcggca atgccgcggg taacgcgctc aaggactatg atctcggcac
     5101 gttcggcaac tcgatctccg gtgcgatcgg cggcgttgcc ggcggcaccg tccttcagtc
     5161 gctcattccg atgctcgcgg gggccggcgg cggcctggat gttggtgcgc tgctcgggca
     5221 ggccgtgggc ggcggtgtgg cgggtgcgat cctcacgttc atcgtcggcc tggtgaagaa
     5281 catgatggcg ggccagccgg cgcgatgagg cagaatttta tcgatccgcg cgcgacctcg
     5341 tgccgcgcgc ggattacttg agggacgggg cgcatcgggg cggcgccagt ggaagctttt
     5401 tctcagggag caagaaatga gcagtgaaaa tagggatgcc aacaaccgca agcgcctgtt
     5461 cggaaccact acgctcgcag cggccgcgat cggactttgt gcatcaattt tcacctcgga
     5521 a
//
LOCUS       DASSRL010000497         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_170389, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000497 DASSRL010000000
VERSION     DASSRL010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170389"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFL49_10085"
     CDS             complement(<1..303)
                     /locus_tag="VFL49_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HET7912370.1"
                     /translation="MSDPAAIKSALAEEARGHGFDAFGIVRPDTAPELKKRLADYIAS
                     GAHGDMTWLAAGADRRASPLAMWPQVRSIVMLGLNYGPDDDPLAILQAADRAAISVY"
     gene            complement(300..989)
                     /locus_tag="VFL49_10090"
     CDS             complement(300..989)
                     /locus_tag="VFL49_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7912371.1"
                     /translation="MLTLFHHPLCPRSRYVRLIFGEYGIAARLVEERFWERREEFLLL
                     NPAGELPVLVTEGQPPIPGAAVIAEYVEETHPQNGSDRLLPDPAGRRVEVRRLANWFN
                     DKFYVEVSGPLVQERAFKRHMTQEQGGGPPDTDALRAARHNIRYHLAYIGWLVRTRDW
                     LAGDRLSLADLSAAAHLSAADYLGEVPWNEDEATKNWYARVKSRPSFRALLADTLAGL
                     PPSKSYADLDF"
     gene            1162..1968
                     /locus_tag="VFL49_10095"
     CDS             1162..1968
                     /locus_tag="VFL49_10095"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7912372.1"
                     /translation="MHLDLIKAAVLGIVEGLTEYLPVSSTGHLLLMEHLFGWGDDAFG
                     KSFAILIQLGAILALLSIYFGRLWALAKAMFTQWEAARFVIGVLLAFLPAAVVGALAY
                     RVIKSYLFDVHVVCISLVIGGFVLLWVDRMNLKARYHDATAFTLPMYLGIGIIQCISM
                     IPGVSRAGATIVGAMLFGADRRSAAEFSFWLAMPTMVGAFAYEIFKSRGDLAAAGNIA
                     VTAVGFAFSFIFGWIVVKTFLGYVQRHSFALFAWWRILVGGIGLIALYIL"
     gene            complement(1976..2950)
                     /locus_tag="VFL49_10100"
     CDS             complement(1976..2950)
                     /locus_tag="VFL49_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET7912373.1"
                     /translation="MSARSPYDTLVTVYGGSGFLGRHVVRALAKRHYRIRVAVRRPEL
                     AGHLQPLGRVGQIHAVQANLRHAASVEAAARDAQVLVNLVGILFERGRQRFQTVHSHG
                     AEQVALAASAHGARLVHVSAIGADTNSPSIYARTKADAESLVLAAQPSASIMRPSILF
                     GPEDDFFNRFAALARLSPALPLVGGGLTRFQPVFVGNVARAIADAVDGATRPGTIYEL
                     GGPDVRTFKELMQFVLRTIERKRLLVPLPFFLAKMQATVLQYFPKPPLTPDQVELLRT
                     DNVVSDAAKAELRTLQGLGIEPETIEAIVPSYLWRFRRTGQFRSRVVA"
     gene            3078..3162
                     /locus_tag="VFL49_10105"
     tRNA            3078..3162
                     /locus_tag="VFL49_10105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3112..3114,aa:Leu,seq:cag)
     gene            3185..>3631
                     /locus_tag="VFL49_10110"
     CDS             3185..>3631
                     /locus_tag="VFL49_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET7912374.1"
                     /translation="MTIRLHRGDLPNLSAYKGSVAIDTEAMGLDPNRDRLCVVQLSPG
                     DGSADVIQIGAGQHKAPNIEKLLADKSILKIFHFGRFDMGILAKTFGVVAEPVYCTKI
                     ASRLVRTYTDKHGLKDLAREVLGVDLSKQQQSSDWGAEELSDAQLSY"
BASE COUNT          698 a         1097 c         1097 g          739 t
ORIGIN      
        1 atagaccgaa attgccgcac gatcggcagc ctgtaagatc gccagtggat catcatctgg
       61 cccatagttc agcccgagca tgacgatcga gcgtacctgc ggccacatag cgaggggcga
      121 agcacgccgg tccgcgcccg ctgccagcca ggtcatatcc ccgtgtgcac cactggcgat
      181 atagtctgca agccgttttt tgagttccgg cgcggtgtcc ggcctgacga tgccgaacgc
      241 gtcgaatccg tgcccacgcg cctcttcagc aagagccgac ttgatcgcgg cgggatcgct
      301 caaaagtcga gatcggcgta gcttttcgat ggcggcaggc ccgcgagggt gtcggccaga
      361 agcgctcgaa acgaagggcg tgactttacg cgcgcatacc agttctttgt cgcttcgtct
      421 tcgttccacg gcacttcgcc caggtagtct gcagctgaaa ggtgcgccgc cgccgacaga
      481 tcggccaaac tcagacggtc gcccgccagc cagtcgcgcg tgcgcaccag ccagccgata
      541 taggccagat gatagcgtat attgtgacgg gcggcgcgca gcgcatcggt gtccggcggg
      601 ccgccaccct gttcctgcgt catgtgacgt ttgaatgcgc gttcctgtac aagcgggccg
      661 ctgacctcga cgtagaactt gtcgttgaac cagttcgcga gtcgtcgcac ttcgacccgc
      721 cgccccgccg ggtccggcag caggcgatcc gatccgttct gcggatgggt ttcttcgaca
      781 tattccgcga tgaccgccgc acctggtatc gggggctggc cttctgtcac aagtaccggc
      841 aactcgccgg ccggattgag cagcaggaat tcctctctgc gctcccaaaa ccgctcttcc
      901 acgaggcggg cggctatacc gtactcaccg aaaatcaagc gcacgtaacg cgaacgcggg
      961 cagagcgggt gatgaaacag cgtcagcata agcgatcaat ctgaaaaaag acggcgggaa
     1021 tcaacgacgc actatagaaa ggcacggcgt ccgcgcaaat catcacaaat attttcacct
     1081 tttttcgttg ccagtcatcc tgctttgggc caaaagcgct ggcccgtttc gttgcccatc
     1141 cgcaaatttc cggagccgtt catgcatctc gacctcatca aggcggccgt gctcggtatc
     1201 gtcgaggggc tgacggaata cctcccggtc tcatcgacgg gccatctgct tttgatggag
     1261 catttgttcg gctggggaga cgatgcgttc ggcaaatcgt tcgcgatcct gatccagctt
     1321 ggcgccattc tcgcgctgct ttcgatttat tttggccggc tttgggcgct ggcgaaggcg
     1381 atgttcacac agtgggaggc ggcacgtttt gtcattggcg tgctccttgc ctttttgccg
     1441 gcggcggtgg tcggagcact cgcctatcgc gtcatcaaat catacctgtt tgacgtgcac
     1501 gttgtgtgta tttcgctcgt catcggcggc ttcgtcctgc tttgggtcga ccgcatgaac
     1561 ctgaaggctc gctaccacga tgcgacagcc ttcacgttgc cgatgtatct cggcatcgga
     1621 attatccagt gcatctcgat gatcccgggt gtgtcgcgtg cgggcgcgac catcgtgggc
     1681 gccatgctgt tcggtgccga ccgccgttcg gctgcggaat tctcgttttg gctcgcaatg
     1741 ccgacaatgg tcggcgcgtt tgcgtacgag atcttcaaaa gccgcggaga tctcgccgcc
     1801 gcgggaaata tcgccgtaac cgctgtcggc ttcgctttct cgttcatctt tgggtggatc
     1861 gtcgtcaaga cgttcctggg ctacgtgcag agacacagct ttgcgctgtt tgcctggtgg
     1921 cgcattctcg taggcgggat cggactgatt gcgctctaca ttctgtagaa tagcatcagg
     1981 cgacgacgcg gctgcggaac tggcctgttc tgcgaaaacg ccacaaataa gagggcacga
     2041 tcgcttcgat cgtctccggc tcgatgccaa gaccctgcag cgtgcgcaac tccgcctttg
     2101 cggcgtcgga cacgacattg tcggtacgca gcaattcgac ctggtcgggc gtgagcggcg
     2161 gcttgggaaa atactgcagg actgtcgcct gcattttcgc aaggaagaag ggtaaaggca
     2221 ccagcagacg tttgcgctcg attgtccgga gcacgaattg catgagctcc ttgaatgtcc
     2281 gcacgtcggg tccgccgagt tcgtagatcg tgcccgggcg cgtggcgccg tcaacggcat
     2341 cggcgatagc ccgcgcaacg ttgccgacga acaccggctg aaaacgtgtc agaccgccgc
     2401 cgacaagcgg cagtgccggc gaaagccgcg caagcgcagc aaaacgattg aagaaatcat
     2461 cctccggccc gaacaggatt gacggccgca taatggatgc cgatggctga gccgccagca
     2521 ccagactttc ggcatccgcc ttggtgcggg cgtagatgct tggcgaattg gtgtccgcgc
     2581 cgatcgccga tacgtggacc agccgcgccc cgtgcgcact cgccgccaat gcaacctgct
     2641 ccgcgccatg actgtgcacc gtttggaaac gctggcggcc gcgctcgaac agaatgccca
     2701 cgagattgac gagaacctgc gcatcgcgtg cggcagcctc gaccgaggcg gcgtgccgca
     2761 gattggcctg caccgcgtgg atttggccga cacggccgag cggttgcaaa tgtcccgcaa
     2821 gttcgggccg gcgaaccgct acccgtatgc gataatggcg tttggccaga gcgcgaacca
     2881 cgtgacggcc gagaaatccc gatccgccat agacggtgac gagggtgtcg taagggctgc
     2941 gtgcgctcat gccgctctcg attttcccgc ggagatcctt ggaaacccgg ccaatacggg
     3001 atcggcgcgg ggctgtacag aaccggaaaa gccgttcatt gacaagccgg aagtggctcc
     3061 gtatctggtc gcaacgagcc caggtggcgg aattggtaga cgcactagtt tcaggtacta
     3121 gcggtgaaag ccgtggaggt tcgagtcctc tcctgggcac caacgtcccg agccgaagaa
     3181 cccaatgacc atccgcttgc accgcggcga ccttcccaac ctctcggcct acaaggggtc
     3241 ggtcgcgatc gacactgaag caatgggcct cgatccgaat cgtgatcggt tgtgcgtcgt
     3301 tcagttgtca ccgggtgacg gatccgccga tgttatccag atcggcgccg gtcagcacaa
     3361 ggcgccgaac atcgagaagc ttctggccga caaatcgatt ctcaagatct ttcactttgg
     3421 ccgcttcgac atgggcattc tcgcaaagac ttttggtgtc gtggctgagc cggtctattg
     3481 caccaagatc gcctcgcgac tcgtgcgcac ttataccgac aagcacggcc taaaggatct
     3541 ggcgcgcgag gtgctcggcg ttgatctatc caaacagcag caatcatccg actggggcgc
     3601 cgaagaactc agtgatgcgc agttgtccta c
//
LOCUS       DASSRL010000498         7356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_362912, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000498 DASSRL010000000
VERSION     DASSRL010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362912"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFL49_10115"
     CDS             <1..244
                     /locus_tag="VFL49_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912375.1"
                     /translation="VPSDDDKNSENVKQNGYFELIVRGIGNLQHMRWPIRFAQCHVTW
                     MHCTTPEHSDPVISHPEKQTERERDLNEKRQQDDNN"
     gene            complement(305..1795)
                     /gene="guaB"
                     /locus_tag="VFL49_10120"
     CDS             complement(305..1795)
                     /gene="guaB"
                     /locus_tag="VFL49_10120"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7912376.1"
                     /translation="MAGKSNAGPREGLTFDDVLIKPGLSEVLPSDVDIRSRITGSIPV
                     NIPIIASAMDTVTEAQMAIAMAQAGGIGVLHRNMEPDVQAAQVRQVKKFESGMVVNPL
                     TIEPDATLQQALDLMKDNHISGIPVVERAIGSQPGKLVGILTNRDVRFATDPRQPVSE
                     LMTKKLITVREGVGQEEAKRFLHQNRIEKLLVVDAQYRCVGLITVKDIEKAVANPNAC
                     KDEQGRLRVAAATTVGDKGFARTEALIAAGVDLVVVDTAHGHSKYVLDAVTRIKRQSN
                     KVQVVAGNVATSEGAKALIGCGADAVKVGIGPGSICTTRIVAGVGVPQLTAIMDCVDA
                     AKASNIPVIADGGIKYSGDLAKAISAGADCAMVGSLLAGTDETPGEVFLYQGRSYKTY
                     RGMGSVGAMSRGSADRYFQQDIKDTLKLVPEGVEGQVPYKGAAATVLHQLTGGLRAAM
                     GYVGAKNLAELHDRAEFIRITGAGLRESHVHDVTITRESPNYPSRV"
     gene            2053..3471
                     /locus_tag="VFL49_10125"
     CDS             2053..3471
                     /locus_tag="VFL49_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912377.1"
                     /translation="MPRDRIVPLIIAVALFMENMDSTVIATSLPAIATDIGTNPLALK
                     LAVTSYLLSLAIFIPASGWTADRFGARAVFRAAIAVFVLGSIGCALSGSLTDFVVARI
                     AQGMGGAMMTPVGRIVLVRTISKRELVGAMVWVTTPALVGPVLGPPVGGFITTYATWH
                     WIFIINVPIGLLGIILATRYIPDVRAEEHERFDVIGMVLAGLGIGGMAFGLSVLGLNF
                     LPWTVVAALVFGGVCFFIAYLAHARRIDNPVLDLSLFRIPTFFASVCGGFIFRLGLGA
                     LPFLLPLMLQIGFGMTPFQSGMITFATALGAMGMKWATASILRWFGFRNILVVNSIIS
                     AAFLAVCAGFTARTPIEVMMLLLFVGGFFRSLQFTSINTIAYADVEPGRISRATALVS
                     VAQQLAVSAGVAVGALAVELTVYFKGSNALQAADFPPAFLAVAAISALSVLIFAQLSP
                     EAGAEMADRLPTPAETSDQRAG"
     gene            complement(3468..4169)
                     /locus_tag="VFL49_10130"
     CDS             complement(3468..4169)
                     /locus_tag="VFL49_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET7912378.1"
                     /translation="MTVRGKSRGPRQLKVRVRSGKNRSQSSKFWLERQLNDPYVARAK
                     REGMRSRAAYKLMEVDDKAHFLKKGARVVDLGAAPGGWSQVAARRTGAPEQGRVVAID
                     ILPMDPIPGVDFAGLDFLDQSAPARLKDMIGGPADVVLSDMAANATGHARTDHLKIMG
                     LVEAAAEFAREVLAPGGVFLAKVLQGGTEAALLASLKRDYRTVKHVKPAASRADSAEL
                     YLLATGFRGAAGDRT"
     gene            complement(4166..5284)
                     /locus_tag="VFL49_10135"
     CDS             complement(4166..5284)
                     /locus_tag="VFL49_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET7912379.1"
                     /translation="MSGEAGDPPGLDPAIDRAPPPRARWGRRSPGRANGRFGNDRGGA
                     TYAALDLGTNNCRLLVARPSGEGFRVVDAFSRIVRLGEGVSSSRRLSEAAIGRAIAAL
                     AICRSKMKNKGVTRARLIATEACRTAENGGEFLARVADELGLALEVIDRETEARLAAT
                     GCTPLIDPEADGAVLFDIGGGSSELVRLERSQRSRCGPPLPRIRGWISVPHGVVTLAE
                     RYGGVTVTRESYDAMVVEVAALIAPFVREHSCAQAEGIHMLGTSGTVTTIAGVHLNLK
                     RYDRNRVDGCWMSADEINAVVERLLMMSYEDRVASPCIGAERADLVLAGCAILEAIRR
                     AFPCPRLRVADRGLREGMLVQMMREDGAWGGNSQATAS"
     gene            5481..>7356
                     /locus_tag="VFL49_10140"
     CDS             5481..>7356
                     /locus_tag="VFL49_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7912380.1"
                     /translation="MDDRTTEIALSLTKFGVGQPVPRTEDPTLVRGQGRYTDDVSRPN
                     QAYAVMVRSRDAHGVIRSIDTAAAKAMPGVLAVYTAADLAGYGPLKCNLPLKNRDGSP
                     IRYTPRPALAADKVRFVGDPVACVIADTVAQAKDAAEAVTLDIEPLPAVLKPSEAAKP
                     GAPLIFDDVPGNVALDYHYGDAARVAEAFAKAKHVTRLETSNQRMVVNAMEPRAAIGE
                     YDAASAKWTLYSTSQGVHGMKTSLMEILGASADKVRVVTGQVGGSFGMKAAVYPEYVC
                     LLLGARLLGRPVKWTDERTGSFVSDHHGRAQDMVVEMAFDDDAHILAVRLTGYGDMGG
                     YLSQFGPLLSTGNQVKNIASVYRTPLIEVATKCVFTNTTFVSAYRGAGRPEGNYYIER
                     TLDLAAAELGIDRVDLRRRNMIRKSDLPFKAASAMTYDCGDFLGVLKQALDAADYAGF
                     NKRKRDSRKRGLLRGFGIGCYLEVTAAPGKELGAIHFEADGTVTIVTGTLDFGMGHAT
                     TYAQILTDLLGVPFDRIRTVEGDSDRMAFGGGSGGSRSVMFVGAALTESAAIVIERGK
                     QIASHVLEASPSDIEFQAGRFVIAGTDRSIELLELAARLRAGLNLPENIPNSLDVDHV
                     V"
BASE COUNT         1317 a         2385 c         2211 g         1443 t
ORIGIN      
        1 cgtgccgagt gatgatgaca agaatagtga gaacgtaaaa cagaacgggt atttcgagtt
       61 gattgtgcgc ggcattggca acctgcagca catgcggtgg ccaattcggt tcgcgcaatg
      121 ccacgtcacg tggatgcact gtaccactcc ggagcacagt gacccggtga taagccatcc
      181 agaaaaacaa accgaacgtg agcgcgactt gaacgaaaag cggcagcagg acgacaacaa
      241 ctgacattaa cgcctccggc actacttgac acgagccggt ccgcaggttg agcgcaaagc
      301 ggtctcagac ccggctcgga taattcggac tttcgcgggt gatcgtcaca tcgtgcacgt
      361 ggctctcgcg caaacctgcg ccggtgatcc ggataaactc cgccctgtcg tgcagttccg
      421 caagattttt ggctccgaca tagcccatgg cggcgcgcaa gccgccggtg agctgatgca
      481 gcacggttgc cgctgcgcct ttgtacggga cctggccctc gacgccttcg ggcaccagct
      541 tgagcgtatc cttgatgtcc tgctggaaat atcggtcggc cgagccacgc gacattgcgc
      601 cgaccgaccc catgccgcga taggtcttgt acgagcggcc ctgatagaga aagacctcac
      661 cgggtgtctc gtcggtgccg gccagaagcg aaccgaccat tgcgcaatcg gcgccggcgg
      721 aaatcgcctt cgccagatcg ccggaatatt tgatgccgcc atcggcgatg accggaatat
      781 tgcttgcctt cgccgcatcg acgcaatcca tgatcgccgt caattgcggc acgccgacgc
      841 cggcaacaat gcgagtcgtg cagatcgagc ccggaccgat gccaaccttg acggcatcgg
      901 caccgcagcc gatcagcgcc tttgcccctt cgctggtcgc aacattcccg gcgaccacct
      961 gcaccttgtt cgactgacgc ttgatgcggg tgaccgcatc gagaacgtat ttcgaatgac
     1021 cgtgcgcggt atcgaccacc accaggtcga caccggcggc gatgagcgct tcggttcgag
     1081 cgaagccctt gtcgccgacg gtggtcgccg cggcaacacg gagccggccc tgttcgtcct
     1141 tgcaggcgtt cggattggca accgccttct cgatgtcttt caccgtgatg aggccgacac
     1201 agcgatattg ggcatcgacc accagaagct tctcgatgcg attctgatga aggaagcgct
     1261 tggcttcctc ctgaccgacg ccttcccgca ccgtgatgag cttcttggtc atcagctcag
     1321 agacaggttg tcgcggatcg gtggcgaaac ggacgtcgcg gttggtcaga atgccgacca
     1381 gtttgccggg ctgcgagccg attgcgcgct cgaccacggg aatgccggag atgtgattat
     1441 ccttcatcag gtcgagcgcc tgctgaagcg ttgcgtcggg ctcaatggta agcgggttca
     1501 ccaccatgcc ggattcgaat ttcttaacct ggcgaacctg cgcggcctgc acatccggct
     1561 ccatgttgcg gtggagcacg ccgatgccgc cggcctgcgc catggctatc gccatctgcg
     1621 cttcggtgac cgtatccatc gccgatgcga tgatcggaat attcaccgga attgagccgg
     1681 tgatccgtga ccggatgtcg acgtcggagg gcagaacttc ggacaggccg ggcttaatca
     1741 gcacgtcatc gaaagtgagc ccttcgcgag ggcctgcatt gctttttccg gccattgcca
     1801 actccgtctt gcccaaaatg gcagtcggac cgtcccgcag tgggttaaaa ctccccgttg
     1861 cgaggggttc ctcgctgcgt cacggcccgc ttttgggttg gcgtgggtcg atagcacggg
     1921 atggcgccga ttcctagcgc aatcacggat ggctgttatg aagccgtaaa cgtgatggag
     1981 ccatctttcc gccgccgctc tacgcttcaa gacgcgctcc tttcaatgac gctcaagccc
     2041 cgtccgttct ccatgccccg tgaccgcatc gtcccgctga tcatcgcggt cgcgctgttc
     2101 atggagaaca tggattcgac cgtcatcgcc acatcgttgc cggcgattgc aaccgacatt
     2161 ggtaccaatc cactggcgct caagctcgca gtgacgtcgt acctgctctc gctggcaatc
     2221 ttcattcccg cgagtggctg gactgccgac cgttttggcg cccgcgccgt ttttcgtgcg
     2281 gccatcgcgg tctttgtgct cggctcgatc ggatgcgctc tgtcggggtc gctcaccgat
     2341 ttcgtcgtcg cccgcatcgc gcagggtatg ggcggagcga tgatgacccc ggtcggacgc
     2401 attgtgctgg tgcgtaccat cagcaagcgc gagcttgttg gagccatggt ctgggtcaca
     2461 acgcccgcgc tggtcggacc ggtgctgggt ccgccggtcg gcggcttcat cacgacctat
     2521 gcgacctggc actggatctt catcatcaat gtcccgatcg gtctgctcgg gattatcctc
     2581 gcgacccgtt acatacccga cgtccgcgcc gaagagcatg aacgcttcga cgtgatcggc
     2641 atggtgctgg cgggtctcgg catcggtgga atggcgttcg gcctttccgt gctgggtctc
     2701 aacttcctgc cctggactgt cgtggccgcc ctcgttttcg gtggcgtctg ttttttcatt
     2761 gcctatctcg cgcatgcgag gcgtatcgac aatcctgtac tcgacctgtc gcttttccgc
     2821 atcccgacat tcttcgccag cgtctgcggc ggattcattt tccggcttgg acttggtgcg
     2881 ctgccgttcc ttctgccgct gatgctgcag atcggcttcg gcatgacgcc attccagtcc
     2941 ggcatgatca ccttcgctac cgcgctcggc gccatgggaa tgaaatgggc aaccgcgagc
     3001 attttgcgat ggttcggctt ccgcaacatc ctggtggtca attccatcat cagcgccgcg
     3061 tttctcgcgg tgtgcgccgg attcacggcc aggacaccga tcgaggtgat gatgctcctg
     3121 ctgttcgttg gcggattttt ccgttcgctg caattcacca gcatcaacac catcgcctac
     3181 gccgatgtcg agccaggacg catcagccgg gcgaccgcgc tcgtaagcgt cgcgcaacag
     3241 ctggccgtat cggctggcgt ggcagtcggg gcgctcgcgg tggaactcac cgtctacttt
     3301 aagggaagca atgcgctgca agctgctgat tttccgcccg ccttcctggc agttgcggcg
     3361 atttcagcgc tctcagtcct gatcttcgcg cagctttcac cggaggccgg tgcggaaatg
     3421 gcggatcggc tgccgacccc ggctgagacc tcggaccagc gcgccggcta ggtccggtcc
     3481 ccggctgcac cgcgaaaccc cgtggcaagc agatagagtt cagctgaatc cgcacggctc
     3541 gccgccggct tcacgtgctt gaccgtcctg tagtcgcgtt tgagagacgc cagcaacgcc
     3601 gcctccgtgc cgccttgcag gaccttcgca aggaagacgc cgcccggcgc cagcacctct
     3661 cgggcaaatt ccgcagcggc ttcgaccaat cccatgatct tcaggtgatc ggtcctggcg
     3721 tggccggtcg cgttggcagc catatcggac agcaccacgt cggccggtcc gccgatcatg
     3781 tccttgagcc tcgcaggcgc gctttggtcg agaaaatcca ggccggcgaa atcaacgcct
     3841 ggtatcggat ccatcggcaa gatgtcgatt gcgaccactc ggccctgctc cggcgcgcct
     3901 gtccgccttg ccgcgacctg actccacccg cccggcgcgg cgccgagatc gaccacgcgc
     3961 gcgcctttct tcagaaaatg cgccttgtcg tcgacctcca tcaatttgta tgcggcgcgc
     4021 gatcgcatgc cttcgcgctt ggcgcgcgcg acataggggt cgttgagttg acgctccaac
     4081 cagaattttg acgactggga gcggtttttc ccgctgcgga cccgcacctt gagctggcgc
     4141 ggcccgcggc tctttccgcg aaccgtcacg acgccgtggc ctggctgttg ccgccccagg
     4201 cgccgtcctc gcgcatcatc tgcaccaaca tgccctcgcg cagtccacga tcggcaaccc
     4261 gcaaccgcgg acacggaaag gcgcgccgga tcgcctcaag tatcgcgcaa ccggcgagca
     4321 cgagatcagc ccgctcggcg ccgatgcaag gacttgcaac gcgatcctcg tagctcatca
     4381 tcagcaatcg ttcgaccact gcgttgattt cgtccgcgct catccagcag ccgtccacgc
     4441 gattgcgatc gtaacgtttc aggttgagat gcaccccggc aatggtggtg accgtgccgg
     4501 acgtgccgag catatgaatg ccttcagcct gggcacagct gtgctcacgc acgaacggcg
     4561 caatcagagc agccacttcg acaaccatcg cgtcgtagga ttcgcgcgta accgtaacgc
     4621 cgccatagcg ctccgccagt gtcacgacgc cgtgcggcac ggaaatccag ccacgaatcc
     4681 ggggcaacgg cgggccgcag cggcttcgct gcgagcgttc gagtcgcacc agttcggacg
     4741 agccgccgcc gatatcgaac agaacggcgc catcggcctc cggatcgatc aggggcgtgc
     4801 agccggttgc ggccaggcgc gcctcggtct cgcgatcgat cacttcaagc gcaaggccaa
     4861 gctcgtcggc gacgcgcgcc aagaactcac caccgttttc agctgtccgg caagcctcgg
     4921 ttgcgatcag gcgcgcgcgg gtgacgcctt tgttcttcat cttgctgcgg caaatggcca
     4981 atgcggcgat cgcacgtcca attgccgctt cgctcagtcg acgcgacgac gagacccctt
     5041 cgccgagccg tacgatcctg gaaaatgcgt cgacgacgcg gaagccttcg ccagacggcc
     5101 gcgcgaccag caaccggcaa ttgttggtcc ccaggtcgag cgcggcataa gtcgccccac
     5161 cccggtcatt gccgaagcga ccattggccc gtccaggcga ccgccgaccc caccgggccc
     5221 gcggtggcgg tgcccgatca atagcggggt cgaggcctgg ggggtccccg gcctcgccgc
     5281 tcatctccaa aaacctttcc tctctgcacc gttaatccgc gatttcccgg gcgcgttacg
     5341 gttgcagcca tgttccttca atcacgaaaa gtgtaacagc gcctgtgcct cgcgcaaccc
     5401 tccgtcgcaa aagctattct tgtagaagga tgcagcgtgc tctatttgac gacgccggcc
     5461 cggaaagcat caccccttca atggatgatc gcaccactga aattgcgctt tcgctgacca
     5521 agttcggtgt cgggcagccg gtgccgcgca ccgaggaccc gacgctcgtg cggggccagg
     5581 gccgctatac cgacgacgtc agccggccaa accaggccta tgcggtcatg gttcgaagcc
     5641 gcgatgcgca cggcgttatc cgctcgatcg acaccgctgc agcgaaggca atgcccggcg
     5701 ttcttgccgt ctataccgcg gcagacctcg ccggttatgg cccgctgaaa tgcaaccttc
     5761 ccttgaagaa ccgcgacgga tcgccgatcc gctacacgcc gcggccggcc cttgccgccg
     5821 acaaagtgcg gttcgttggc gatcctgtcg cctgcgtgat cgccgacact gtggcgcagg
     5881 caaaagacgc agccgaagcg gtgacgctcg acatcgagcc gctgccggcg gtgctcaagc
     5941 catcggaagc ggccaaaccg ggggcgccgc tgatattcga cgacgtgcct ggcaacgtcg
     6001 cgcttgatta ccactacggc gacgccgcca gggtcgcgga agcctttgca aaagcaaagc
     6061 acgtgacacg gcttgagacg tccaaccaac gcatggtggt caacgctatg gagccgcggg
     6121 ccgccatcgg tgaatacgac gcggcctcgg caaaatggac gctctactcc acgagccagg
     6181 gcgtgcacgg aatgaagacc tcgttgatgg aaattcttgg cgcgtctgcc gacaaggttc
     6241 gcgtggtcac cggtcaggtc ggcggctctt tcggcatgaa ggccgcggtc tatcccgagt
     6301 acgtctgcct cctgctcgga gcgcggctgc tcggacgacc tgtcaaatgg acggacgagc
     6361 gcactggcag tttcgtgtca gaccatcatg gtcgcgcgca agacatggtc gttgagatgg
     6421 cgttcgacga cgacgcgcat attctcgcgg tccgcctcac cggctacggc gacatgggcg
     6481 gttacctgtc gcaattcggc ccgctgctgt cgacgggaaa tcaggttaag aatatcgcca
     6541 gcgtgtaccg cacgccgctg atcgaggtcg cgaccaaatg tgtgtttacc aacacgacct
     6601 ttgtgtcggc ctaccgtggc gccggccggc ctgagggcaa ttactatatc gagcgcacgc
     6661 tggacctggc cgccgccgag ctcggcattg accgcgtcga cctgcgcagg cggaacatga
     6721 tccggaagag cgacctgccc ttcaaggccg catcggccat gacttacgac tgcggtgatt
     6781 ttcttggtgt gctcaagcag gcgctcgatg ccgccgatta tgccggcttc aacaaacgca
     6841 agcgcgacag caggaaacgc ggtctgctgc gcggatttgg catcggctgc tatctggaag
     6901 ttaccgccgc accgggcaag gaactggggg ccattcactt tgaagccgac ggcaccgtga
     6961 cgatcgtcac cggcacgctc gatttcggaa tgggtcacgc aacgacttac gcacaaatct
     7021 tgaccgatct gcttggcgtt ccgttcgacc gcatccgcac ggtcgagggt gacagcgacc
     7081 ggatggcttt cggcggcggc agcggcgggt cgcgttcggt gatgtttgtc ggcgcggcgc
     7141 tgactgaatc agcggcgatc gtgatcgagc gcggcaagca aattgcctct catgtgcttg
     7201 aggcttcgcc gagcgacatc gaattccagg cgggccgctt tgtgatcgcc ggcaccgatc
     7261 gttcgatcga gctgctcgaa cttgccgcgc ggctgcgcgc aggcctcaac ttgccggaga
     7321 atatcccgaa ttctctcgat gtcgatcatg tcgtca
//
LOCUS       DASSRL010000499        10174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_503057, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000499 DASSRL010000000
VERSION     DASSRL010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503057"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1138
                     /locus_tag="VFL49_10145"
     CDS             104..1138
                     /locus_tag="VFL49_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7912381.1"
                     /translation="MLRGAFIGFGNVAAKGHLPGWQSVKDVQIVAATDAVEAQGSAFL
                     EACPGGRWHESVDSLLADKTLDFVDICTPPSSHAALIKRALDAGLHVLCEKPLVTRIG
                     DAQAVAAAAAKSGRAVHTVHNWLKAPICDRISSLVDEGAVGSVQTVHWQTLRTQPAIT
                     VTSDGSNWRADPAVAGGGILFDHGWHALYCVMRWSGIPRGVSALLEKRRFREWPIEDT
                     ATVTLDLMSGSAQIFLTWAANERANRIEIEGDRGRIEVADDVVVLKSGQGERKWPCPP
                     PLSQGSHHRDWFIGVAQDFRRAATAGGKGNLDEAVLCARLIDLAQRSSAAGGVRLPLE
                     ALTHREEYQQ"
     gene            complement(1070..2329)
                     /locus_tag="VFL49_10150"
     CDS             complement(1070..2329)
                     /locus_tag="VFL49_10150"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7912382.1"
                     /translation="MQQGSHNVAPSPPVMVLLPDSGGSRLVSTFAAGGEPRVLGLGLA
                     RRTVMTAGRAGYNRIYFLARDQAAPSGITAISNWSSLADAFSLQPGPLIIAHAAILAE
                     PEWLARLIGTRIEPARWAVLPHKMILLAAGAVAEAVAALDADKGGYDLTAAQERLTRR
                     FGAPAAVPAAIDPLVVTTPTDVDDAEWRLLRALVKDTDGFMARHVERPMSLRIARRLA
                     SSGITPNQVTLLSIGIGLLGAPCFLSTHWLWQTAGAILFLAHSILDGCDGELARLKFQ
                     ESRFGGIIDYWGDNIVHIAVFACMAVGWSLSVSASWPLLLGVAAVLGSLGSAWFVYWR
                     TMHAKKNDGPLFTSVATVPGQALARMLDAASRRDFIYLVLFLALFGKSNWFLVLAAVG
                     APIFFLLLVFLAMRERFQRQPDAARRA"
     gene            2484..3413
                     /locus_tag="VFL49_10155"
     CDS             2484..3413
                     /locus_tag="VFL49_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET7912383.1"
                     /translation="MAHSGAGEVANAMLVVGWWLLPITAYHIVPMFFSALSWRELLPP
                     ASRPDVLGVTWIRWIRESINALLPVASIGGDIAGARLAHLRGVPGTQAAASMVVDTTV
                     GVVTQLAFVITGVALLALRPAGHSAFEVVAGVVIGVGVLVAAVAAFVLFQHKNMFAHF
                     ARLARGLLPGKWLSHMAGSASAVDDAVVQAYRRGPAFWRANLLRLIGWAAGAGEVWLV
                     TWCLGQPLAATDAFILESLSSGVRAAAFMVPAALGVQEGGLVLFGALFGLPAEIGLAI
                     SLSKRVRELLLGLPGLFFWYWIEGHYLLRRNGE"
     gene            complement(3307..4182)
                     /locus_tag="VFL49_10160"
     CDS             complement(3307..4182)
                     /locus_tag="VFL49_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HET7912384.1"
                     /translation="MRRSAVWVRWAITLVLPIVVGACAATPRDAALPLQDPNEQANRK
                     MLAMNQEVLRPVAEAVQTVVPRPVHDRLHDFNSNLREPRIFLNNVLQGRLDAASKTAA
                     RFAMNTTFGIGGLVDLASREGIPQQTGDFGQTLFVWGVGDGPYMVRPYFGPTTTRDAV
                     GDLVDRFGDPVGLLFGTQIGIAITTASLDTVVRLGQLKMAEDASIDFYAFLRSSYYQT
                     RRAELRDALGLPNVIDSPATALDETAKPDSPKSTAASASTPRSGVANSAPRSSTKKTA
                     RATQEATRALASTTR"
     gene            complement(4356..6977)
                     /locus_tag="VFL49_10165"
     CDS             complement(4356..6977)
                     /locus_tag="VFL49_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7912385.1"
                     /translation="MSKTVNQLAIGATTQAVRLSTRYAWLVLLAFLLVAILSAAYFTS
                     HFAITTDSNKLLSNSLPWRQQEHALDRAFPWRIDQIIAVVDATTPEAAEEAADVLASD
                     LSRHPDVIRTVFRPDGGEFFARNGILFRSLDEVRRDAADLIAAQPFLGTLSADPTLRG
                     VLRTLSQSIEGLRLGKTKLEDLRPALAAIADALETLANGKYPTFSWRKLITGRVPEAS
                     ELRRFINIQPVLDFDDLQPGRKATATIREAASRLGLTPERGVKVRLTGSVALADEEFA
                     TIADGAALNGAITLLLVMLVLWLALKKARIILAVVINLATGLIFTAAIGLWMVGALNL
                     ISVAFAVLFVGLGVDFGIQFSVRYRAERFASHDLEEALLGTAKGLAGPLLLAAASIAA
                     GFYSFLPTAYVGLSELGLIAGTGMIVAFATTVTLLPALLAILKPPGEPAPIGYAALAP
                     VDRFLETQRKWVVGTTLAVAILGLPLLAGLRFDFNPLDLRSKDTESVATLLDLMADPD
                     TSPNTLDILESNGARASALAERLRRLPEVAHVVTLQSFVPKDQDEKLAIIDDASFFLR
                     NTLNPDQVEPKPTAADTLAAIETTARDLRGAAADTDNSPGAIQSRRLAGLLATLTKQS
                     QTAREVAERVFVMPLVTTLRQVRSLLTAEHVTIETLPRSLTRRWVSADGLNRIEVAPS
                     GDGNDNVTLERFVAAVRNVAPDAAGKPVFVIEAAATIVKAFIQAAIWSVASITIILFV
                     VLRRWSHVALTLVPLLVAIVATLEICVIIGLQLNFANIIALPLLLGVGVAFKIYYIMA
                     WRSGERNFLQSSLTRAVIYSACTTATAFGSLWFSHHPGTSSMGKLMALCLITTLAAAV
                     LFQPALLATQSKANSSK"
     gene            7145..7765
                     /locus_tag="VFL49_10170"
     CDS             7145..7765
                     /locus_tag="VFL49_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912386.1"
                     /translation="MKAFKVDLRHSIAAVAIVAAVLVVPVRLHAQSSAVAHIQSYYQQ
                     LMPTIQQAGRLTVRERDKRFTPAITSAFDLPTMTRLAVGPSWQSFSPVQQAAVRDAFA
                     KFLIADYASQVKDYSGESFIVDPKPVPEPRGGGEIVKTRLLQPGGRTVTINYLVRGGR
                     VIDVYLNGTISDLATRRDEFAAIIASGGADGLIKRLRDRTQKLLGG"
     gene            7840..8844
                     /gene="hpnA"
                     /locus_tag="VFL49_10175"
     CDS             7840..8844
                     /gene="hpnA"
                     /locus_tag="VFL49_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HET7912387.1"
                     /translation="MKTQDKVLVTGASGFVGSAVAHKLAEAGFSLRALVREKGPHSNL
                     AGLDLEFFEGDLRDRKSLERAIAGVRYVFHVAADYRLWARDPNEIFATNVEGTRNVME
                     AALHAGVERIVHTSSVATLALRSGQVADESTPQPENEAIGAYKRSKIAAERLVDSMVA
                     ERGLPAVIVNPSTPVGPRDVRPTPTGRIIVEAAKGRIPAFIDTGLNLVHVDDVASGHL
                     AALRHGKIGQRYILGGENVLFSQMLKDIAGMVGRRAPWVRLPWRALVPVAFAAEAAAA
                     ITGREPFVTLDGVYLARYRMFFASTKAERELSYRPRPYIEGIEDALQWFRAAGYLKFP
                     "
     gene            8889..9647
                     /locus_tag="VFL49_10180"
     CDS             8889..9647
                     /locus_tag="VFL49_10180"
                     /inference="COORDINATES: protein motif:HMM:NF005476.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HET7912388.1"
                     /translation="MPRPTILAVTSLAREAQIARGNGVSVVCSQGERLGAALRAAIDP
                     GVAGIISFGISGGLNPALVAGDWVIASAVRYSDRVIVTDSKWRQELCKRLPGAVLADI
                     VGSDTMVPTPAEKSHLHRTTGAVAVDMESHIAAEAAAAHGIPFAACRVIIDAAHRALP
                     SAAGPALDSNGTVNIFAVMRSVLRTPSQLPDLARAAFDACIAERALRKGRKQLELGFG
                     SPFVNGRAVERGFAGQPDVSSDFLRPLPPICETP"
     gene            9792..>10174
                     /locus_tag="VFL49_10185"
     CDS             9792..>10174
                     /locus_tag="VFL49_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7912389.1"
                     /translation="MTRDDKKQPGGDQITASFIETAIRLAVLALLLYWSFILVQPFIS
                     IFIWSAVLAVALFPIFEWISFRLGGRRRLAAALTTVLSLLVIIGPATWLAMGLVDSVR
                     MISDRLDPSILAVPSPPVSVKDWPL"
BASE COUNT         1909 a         3070 c         3202 g         1993 t
ORIGIN      
        1 cgctggtgat taccgaggat gaggcactgc gcggcgtcga cattctcgac cagtcgttcg
       61 gtgccatcgc gcgccaattc gggctggact gaggccgcgg cgcgtgttgc gtggcgcttt
      121 tatcgggttt ggaaacgtcg ccgcaaaagg ccacctgccg ggctggcagt ccgtgaagga
      181 cgttcagatc gtcgccgcga ctgacgccgt cgaggcgcag ggcagcgcct ttctggaggc
      241 ctgccccggc gggcgatggc acgagagcgt cgacagtctg ctcgccgaca agacactcga
      301 ttttgtcgat atctgcacac cgccaagcag ccatgcggcg ctcatcaagc gtgcgctgga
      361 tgccggtctc cacgtcctgt gcgaaaagcc tctggttacg cggatcgggg atgcgcaagc
      421 cgtggcagcg gccgcggcga aaagcggccg tgccgtgcat actgtgcaca actggctgaa
      481 ggcgccgatc tgcgacagga tttcgtcgct cgtcgatgaa ggtgcagtgg gcagcgtgca
      541 aacggtccat tggcagacgt tgcgcacgca gcccgcaatc accgtcacat ccgacggcag
      601 caactggcgc gccgatccgg ccgtcgccgg cggcgggata ttgttcgacc acggctggca
      661 cgcgttgtat tgcgtcatgc gctggtccgg catcccccgc ggtgtcagcg cgctgctgga
      721 aaagcgccgc tttcgcgaat ggccgatcga agatacggca accgtcacgc tcgatctgat
      781 gtccggcagc gctcaaattt ttctgacatg ggctgcaaac gagcgcgcca atcgcatcga
      841 aatcgaaggc gatcgcggcc gcatcgaagt cgcggacgac gtcgtggtac tcaaatctgg
      901 tcaaggcgag cggaagtggc cctgcccgcc gcctctttcc cagggatcgc atcatcgcga
      961 ctggttcatc ggggtcgcgc aggactttcg ccgcgctgcc acggccggcg gcaaaggcaa
     1021 tctggacgag gccgtgttgt gtgcccgcct gatcgacctt gcgcagcggt caagcgcggc
     1081 gggcggcgtc cggttgccgc tggaagcgct cacgcatcgc gaggaatacc agcagtaaaa
     1141 agaaaatcgg tgcgccgacg gccgccagaa ccagaaacca gttcgacttt ccaaacagcg
     1201 caagaaagag caccagatag atgaaatcgc ggcgcgatgc ggcatcgagc atgcgcgcga
     1261 gtgcctgccc ggggacggtc gcaaccgaag tgaagagagg gccgtcattt ttcttcgcgt
     1321 gcatcgtgcg ccaatacacg aaccaggccg atcccagact tccgaggacg gcggcgacgc
     1381 cgagcaacaa cggccatgac gccgaaacag acagactcca gccgacggcc atgcacgcga
     1441 agacggcgat atgaacgatg ttgtcgcccc agtagtcgat gattccgcca aaacgcgatt
     1501 cctgaaactt gagcctggcc agctctccgt cgcatccgtc gaggattgaa tgtgcaagaa
     1561 aaagaatcgc tccggccgtc tgccagagcc agtgcgtaga cagaaaacag ggcgcgccaa
     1621 gcaggccgat accgatcgaa agcaacgtaa cctgatttgg cgtgatgccg ctcgatgcga
     1681 gtcggcgggc aattcgcagg gacatcggcc gctcgacatg gcgcgccatg aatccgtcgg
     1741 tatccttgac gagcgcgcgc aacaagcgcc actccgcgtc gtcgacatcg gtcggcgtcg
     1801 tcaccactag cggatcgatg gcagccggaa ctgcggccgg cgccccgaag cggcgcgtga
     1861 gccgttcctg cgccgcggtc aggtcgtatc cccctttgtc ggcgtcgagc gctgcaacgg
     1921 cctccgcgac cgctccggca gcaagcagga tcattttgtg cggcagcact gcccacctgg
     1981 ccggctcgat ccgtgttccg atcagccgtg cgagccattc gggttcggcg agaatggccg
     2041 catgcgcaat aatcagcggc cccggttgca gggaaaacgc gtcggccaga ctgctccagt
     2101 tggaaatggc cgtgatccct gatggcgcag cttgatctcg cgccagaaaa tatatccggt
     2161 tgtagccggc gcggcccgcg gtcatcacgg tacggcgcgc gagtccaagc ccgaggaccc
     2221 ttggctcgcc gccggcggcg aacgtcgaca caaggcgcga cccgccggaa tccggcaaaa
     2281 gaaccattac gggtgggctc ggagccacgt tgtgcgaacc ctgctgcatc ggacttgcga
     2341 tatacctcgg ccggacgccg cgttgatact acaagcggtg cgtctattct tgccgcgttc
     2401 ggtgccgagt aagggggatc cgggtcattt ctgtttcgtt catcatcgcg ggtttctttg
     2461 gccttgcgct tgtcgtctac ctgatcgccc acagcggcgc cggtgaggtg gccaacgcca
     2521 tgctggtcgt cggctggtgg ctcttaccga taacagccta ccacatcgtc ccgatgtttt
     2581 tctccgctct ttcgtggcgg gagctgttgc cgcccgcaag ccgtccggat gttctcggtg
     2641 tgacctggat ccgatggatc cgcgaatcaa tcaacgcgtt gctgccggtc gccagcatcg
     2701 gcggcgacat agccggcgcg cggctggctc acctgcgcgg cgtgccgggc acgcaggcgg
     2761 cggccagcat ggttgtcgac accacggtcg gtgtggttac gcagctggct ttcgtcatca
     2821 ccggcgtagc gctgctcgcg ttgcggcccg ccggccacag cgcatttgaa gtggtggctg
     2881 gcgtggtgat cggtgtcggc gtacttgtcg cagccgttgc ggcatttgtc ctgttccagc
     2941 acaagaacat gtttgcgcac tttgccaggc ttgcgcgcgg cctgttgccc gggaaatggc
     3001 tttctcatat ggcgggaagc gcatcggcgg tcgatgacgc cgttgtgcaa gcctatcgcc
     3061 gaggccctgc cttctggcgg gcgaacctgc tgcgcctcat cggatgggcg gcaggcgcgg
     3121 gtgaagtctg gctggtgacc tggtgtctcg gccagcccct tgcggcaact gatgccttta
     3181 tcctcgaaag cctttcctcc ggcgttcgcg ccgcagcctt catggtgccc gccgcgcttg
     3241 gcgttcagga aggcggtctt gttctgttcg gcgcgttgtt cgggctgccg gcggagatcg
     3301 ggctggctat ctcgttgtcg aagcgagtgc gcgagttgct tcttgggttg cccgggctgt
     3361 ttttttggta ctggatcgag gggcactatt tgctacgccg gaacggggag tagacgcact
     3421 tgccgccgtg ctcttcggcg agtccggttt ggcggtttcg tccagcgcgg tcgccggcga
     3481 gtcgatgacg tttggcaacc caagcgcatc acgcaattcc gcgcgccgcg tctggtagta
     3541 gctcgatcgc aggaatgcgt agaaatcaat ggacgcatcc tcggccatct tcaactggcc
     3601 gagcctgacc acggtatcca ggcttgcggt ggtgatcgcg atcccgatct gcgttccaaa
     3661 caacaagccg accggatcgc caaatcggtc gacaaggtcg ccgaccgcat cgcgcgtcgt
     3721 ggtcggaccg aaatagggcc tgaccatgta ggggccatcg ccgacacccc agacaaacag
     3781 cgtctgtcca aaatcgccgg tctgttgcgg aatgccttcc cggctcgcaa gatcgacaag
     3841 gccgccgatt ccaaacgtcg tgttcatggc aaaacgcgcc gcggtcttgc tggcggcgtc
     3901 gaggcgacct tgcagcacat tgttcaggaa aatccgcggc tctctcaggt tcgaattgaa
     3961 atcgtgcaag cgatcgtgca ccgggcgcgg caccaccgtt tgtacggcct ctgcgaccgg
     4021 gcgcaacact tcctgattca tagcaagcat cttgcgattg gcttgctcgt tgggatcctg
     4081 gaggggcaac gccgcgtccc tcggcgtggc ggcgcaggcg cccacaacga taggcaatac
     4141 gagtgtgatc gcccatctga cccagactgc actgcgacgc atttactttc ccgccccttt
     4201 tcaattcccc tgcaggcccg aggtttgtcg gatcttatga caagcgcggc atgtactccg
     4261 acgtaatgcg ccgatcagat tgagtccgac aaaattagca gagccgctac ggcataagtg
     4321 ttactttttt gtcacaggcc tcggttttag cccatttatt tactgctatt cgccttgctc
     4381 tgagtcgcca aaagtgccgg ctggaagagc acggctgcgg caagcgtcgt gatgagacac
     4441 agggccatca gcttgcccat gctcgatgtg cccgggtggt gtgagaacca caggctaccg
     4501 aaagcggttg ccgtcgtaca ggcactgtag ataacggccc gtgtcaggct cgattgcaga
     4561 aagttccgtt caccggaacg ccaggccatg atgtagtaaa tcttgaacgc gacgcccacg
     4621 ccgagaagaa gcggtagcgc gatgatgttg gcgaagttca gctgcagccc gattatcacg
     4681 cagatttcca gtgtcgcgac gatggcgacg agcaggggta ccaacgtcag cgcgacgtgg
     4741 ctccatcggc gcaatacgac gaaaagaatg atggtgatcg acgccaccga ccagatcgca
     4801 gcctgaatga acgccttgac gatggtggct gccgcctcga tgacgaagac cggctttccg
     4861 gccgcatcgg gcgcgacatt gcggacggct gcgacgaaac gctccagggt cacgttgtcg
     4921 ttgccgtcgc cgcttggcgc gacctcaatc cggttcaggc cgtcggccga gacccagcgt
     4981 cgcgtcagcg accggggaag tgtctcgatc gtcacgtgct cggccgtcaa caggcttctg
     5041 acctgacgca gcgtcgtcac cagcggcatc acgaaaacgc gctcggccac ctcccgcgct
     5101 gtctgagatt gttttgtcag cgttgccagg aggccggcga gacggcgcga ttgaatggcg
     5161 cccgggctgt tgtcggtgtc ggctgcggca ccgcgcagat cacgggccgt ggtctcgatt
     5221 gctgccagtg tgtcggcggc cgtgggtttt ggctcgacct gatccggatt gagcgtgttg
     5281 cgcaggaaaa agctggcgtc gtcgatgatc gcgagcttct cgtcctggtc cttgggcacg
     5341 aaactttgca gcgtcacgac atgcgcaacc tcgggcaacc gacgtaagcg ttccgcaagc
     5401 gccgaagcgc gcgcgccatt gctttccagg atgtcgagcg tgttcgggct ggtgtcggga
     5461 tcggccatca gatcgagcag cgttgcgacc gattccgtgt ctttcgaccg tagatcgagc
     5521 ggattaaagt cgaaacgcaa gcctgcaagc aaaggcaggc cgaggatcgc gactgccaat
     5581 gtcgtgccga cgacccattt gcgctgcgtt tcgaggaagc gatcgaccgg cgccagcgcg
     5641 gcgtagccga tcggtgccgg ctcgccgggc ggtttgagaa ttgcaagcag cgccggcagc
     5701 aatgtcacgg tcgttgcaaa cgcaacaatc atgccggttc ccgcaatcaa tcccagttcg
     5761 gatagtccga cataggctgt cggcaggaac gagtagaacc ctgcggcgat tgaagccgcc
     5821 gccaataaaa gtggaccagc caatcctttc gccgtgccga gaagggcctc ttcgaggtcg
     5881 tgtgatgcaa atcgttcggc gcggtaacgc acgctgaact gaatgccgaa atcgactccc
     5941 agcccgacaa aaagcacggc gaaggcaacg gagatcagat tcagggctcc taccatccag
     6001 agcccgatgg cggcggtgaa gatgagtcca gtcgcgagat tgataactac ggccagaatg
     6061 atccgcgctt tcttcagcgc aagccagagc accagcatca cgagaagaag agtgatagcg
     6121 ccgttcaacg cggctccgtc ggcaattgtg gcgaattcct cgtccgcaag agcaaccgac
     6181 ccggtgagac gcactttcac tccgcgctca ggcgtcagcc cgagccgcga tgccgcctcg
     6241 cggatcgttg cagttgcctt gcgtccgggc tgcagatcgt caaagtccag gacgggttga
     6301 atgttgatga aacggcgaag ctccgaagcc tccggcaccc gtcccgttat caatttgcgc
     6361 caggaaaagg tcggatattt tccattcgcc aaggtttcca gtgcgtcggc aatcgcggca
     6421 agtgccggtc tcaggtcctc cagcttcgtc tttccgaggc gcaatccctc gatcgactga
     6481 gacagcgtgc gaagaacgcc gcgcagcgtc gggtctgccg agagggtgcc gagaaacggc
     6541 tgcgccgcga tgagatcggc cgcatcgcga cgcacttcgt cgagagagcg aaaaagaatg
     6601 ccattccggg cgaaaaattc gccgccatcg gggcggaaaa cggtccggat cacgtcgggg
     6661 tgacgcgaga ggtcgcttgc gagcacgtcc gccgcttcct cggcagcctc gggtgtggtt
     6721 gcgtcaacga cggctatgat ctggtcgatt ctccagggaa aggcacggtc gagagcatgt
     6781 tcctgctgcc gccaaggcaa tgagttcgac aaaagcttgt tgctgtcggt cgtgatcgcg
     6841 aaatggctgg tgaaataggc agccgacagg attgcaacaa gcaagaatgc aagaagcaca
     6901 agccacgcat atcgggtcga tagacgcaca gcctgcgtag ttgcgccaat tgccagctga
     6961 ttgacggtct tgctcattta atttgcgaaa aaagcacatt catttcaggc tgtcgcctac
     7021 gtcttgacgg ggccccaatc caattccaaa aacaacctgc agaaacagct ttgcagcccg
     7081 caccgccgac aggccgcggt cccgtgggca ggccgtggcc caatccggta gactggaaat
     7141 cacaatgaaa gcgttcaaag tcgacttgcg tcattcaatc gcagccgttg ccatcgttgc
     7201 cgccgtcctg gtcgttccgg tccggttgca cgcgcagtca tcggccgttg cgcatattca
     7261 aagctattac cagcagttga tgccgacgat ccagcaagca ggccgcctga ctgtgcgcga
     7321 gcgtgacaag cggttcaccc ccgccattac gtccgctttc gatcttccga cgatgacgag
     7381 gttggcagtg gggccatcgt ggcagagttt ctcgcccgtg cagcaggccg cagtccgcga
     7441 tgcttttgcc aaattcctca ttgccgacta tgcaagccag gttaaggact attccggtga
     7501 aagctttatc gtcgatccga aaccggtgcc tgagccgcgt ggcggtggcg aaatcgtgaa
     7561 gaccaggctg ttgcagcccg gcggccgcac tgtgaccatc aactatttgg ttcgtggcgg
     7621 gcgcgtgatc gacgtctatc tcaacggtac gatcagtgac ctcgcgacac ggcgcgatga
     7681 atttgctgcg attatcgcct cgggtggcgc cgacggtttg atcaagaggt tgcgcgatcg
     7741 gacgcagaag ttgcttggag gctaatctcg ctcgttcgat gccggttcgc cgatgggtcg
     7801 gcacggttcg aaacctcggc atatcggaac ccgcgacgga tgaagactca ggacaaggtt
     7861 ctcgtcaccg gagcgagcgg gttcgtcggc tcggctgtcg cccacaaact cgccgaggcc
     7921 ggcttttcgt tgcgggcgct cgtccgtgag aaaggtcccc actcaaatct cgccggcctc
     7981 gacctggaat ttttcgaagg cgacctgcgc gaccgaaaat cgctcgagcg tgcgatagcg
     8041 ggcgtacgct acgtttttca tgtggcggcc gactatcgcc tgtgggctcg tgatccgaac
     8101 gaaatctttg ccaccaacgt tgaaggcacc cgcaatgtca tggaggcggc gctgcatgcg
     8161 ggcgttgagc ggatcgtcca tacgagcagc gtcgcgaccc ttgcgttgcg gtccggtcaa
     8221 gtcgctgacg aatccactcc gcagcccgag aacgaggcaa tcggcgccta caagcgcagc
     8281 aagatcgcgg ccgagcgcct ggtcgactcc atggtagcgg agcggggcct tccagcggtc
     8341 atcgtcaatc cctcgacgcc ggttggaccg cgcgacgtgc ggcccacgcc gaccggccgg
     8401 attattgtgg aagcggccaa gggccggatt ccggcgttca tcgataccgg actcaacctc
     8461 gttcatgtgg acgatgtggc gagtggtcat ctcgcggcat tgcgtcatgg gaagatcggg
     8521 caacgctaca tcctcggcgg tgaaaacgtt ttgttctcgc agatgctgaa agatatcgcc
     8581 gggatggtgg gccgccgcgc gccgtgggtg cggcttccct ggcgtgccct ggttcccgtc
     8641 gcgtttgctg ccgaagccgc agccgcgatc acaggacgcg aaccctttgt aacgttggat
     8701 ggggtctact tggcgcgata tcgcatgttc tttgcgtcca ccaaagccga gcgggagcta
     8761 tcctaccggc cgcggccata tattgagggg attgaagacg cgctgcagtg gtttcgtgcc
     8821 gcgggatact tgaaattccc ctagggtcgg atacgaatgg gctccgcagt gaccgtcagg
     8881 ggggcgggat gccaagacca acgattcttg ccgtcacctc gcttgcgcgc gaggcacaaa
     8941 ttgcgcgtgg taacggggta tcagtggttt gcagccaggg cgagcgcctt ggcgccgctt
     9001 tgcgcgcggc tatcgaccct ggcgttgcgg gaatcatcag ctttggaatc tccgggggcc
     9061 tgaatccggc gctcgttgcc ggtgactggg tcatcgcatc tgccgtcagg tattccgatc
     9121 gcgtaatagt cactgattcg aaatggcggc aggagctctg caaacggctt ccgggcgccg
     9181 tgcttgcgga cattgtcggc tccgacacca tggtcccgac tcccgcagag aagtcgcatc
     9241 tccaccgaac gacaggcgct gttgccgtcg acatggaatc ccacatcgcg gccgaggctg
     9301 ctgccgcgca cggaattcca tttgccgcct gccgggtgat catcgatgcg gcccaccgcg
     9361 cgcttccgtc cgcggcaggt ccggcgctgg actcgaacgg cactgtgaat atttttgcag
     9421 tcatgcgctc tgtcctgcgg acaccaagcc agcttcccga tctcgctcgc gccgcattcg
     9481 acgcctgcat tgccgagcgg gccttgcgaa agggccgaaa acagctggag cttggatttg
     9541 ggtcgccgtt tgtcaacgga cgggccgtcg agcgcggttt cgcgggccag ccggacgtat
     9601 cgagcgattt cctgcggcca ttgccgccga tttgcgagac cccgtgatcg gctgaaaacg
     9661 gcggcgatcg ccgcgcggca ataacgaccg agcgcggcat cggccgtttt tgcactttcc
     9721 gggtgctgtg cgggagcagc gctccacaac atcaaccggt gcaagcgaac ggcgagtcgg
     9781 gaaagccacc agtgacgcgc gacgacaaaa aacagcctgg cggtgatcag atcacggcgt
     9841 catttattga aacagccatc cggttggcgg ttctcgcgct tctgctctac tggtctttca
     9901 tccttgttca gccatttatt tcaattttta tttggagcgc ggtgttggcc gtcgcgctgt
     9961 ttcctatctt cgaatggatt tcgttccggc tcggagggcg tcgtcgattg gcggccgcat
    10021 tgacgacagt tctaagcctg ctggtcatca tcggtccggc gacctggctt gccatgggtc
    10081 tggtcgacag tgtcaggatg atttccgacc ggctcgaccc ttccatcctg gcagtaccgt
    10141 cgccgcctgt ttcggtgaaa gactggccgc tgat
//
LOCUS       DASSRL010000500         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1658055, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000500 DASSRL010000000
VERSION     DASSRL010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658055"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..2223)
                     /locus_tag="VFL49_10190"
     CDS             complement(115..2223)
                     /locus_tag="VFL49_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET7912390.1"
                     /translation="MNQSVRLPKAVRIGHSACPHDCPSTCALEVEILDERTIGRVYGS
                     KDNDYTAGVICAKVARYAEREHHAERLLYPLRRVGAKGSGKFERISWEDALDLVAEKF
                     IEAERRYGAESVWPYYYAGTMGLVMRDGINRLRHVKKYSGFHSTICVNPAYAGYAAGI
                     GRIAGVDPREMAKSDLVIIWGTNAVNTQVNVMTHAVRARKERGAKIAAVDIYMNGTME
                     QADLPVLIKPGTDGALACAVMHCLFRDGKADWNYLEKYTDAPHELEAHLRSRDPQWAA
                     AITGAPAETIEEFARLIGERKRAFFRLGYGFSRSRNGAANMHAASCIPAVTGAWQHEG
                     GGAFHMNADIFRLDKTMIEGLDVSDHSVRMLDQSRIGAILTGEREALHGGAPVTAMII
                     QNTNPVSVCPDQTQVKRGFAREDLFVCVHEQFMTETAQAADVVLPATTFLEHDDIYRG
                     GGHQYLILGPKLVEPPGECRNNHEVICGLAQRLHAEHRGFTMSPRELIDQMLQVSKRG
                     SLAEIEANKWIDCQPPFRESHFLDGFPWSDGKFRFKPDWPNVPFRSPCKSGPIADMPG
                     LPDYWTSIEVADAEHPFRLATSPARGFLNSTFNETPTSLAQENRPTVMIHPGDAAALG
                     ISTGDYVVLGNKRGEVRLHAHVFKGVRRGVLIAESIWPNAAYADGRGINTLIGADSIA
                     PYGGAAVHDNKVWIRKARAD"
     gene            complement(2250..2984)
                     /locus_tag="VFL49_10195"
     CDS             complement(2250..2984)
                     /locus_tag="VFL49_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7912391.1"
                     /translation="MVSSGWNDMGHAEFWIALAKIMWINILLSGDNAVVIAMACRGLP
                     HRQRIWGMILGAGVAVGLRIIFTGLVASLMLLPYLKIVGGLALFYIAAKLLVPEDADG
                     DEAEAAEHLWRAVRIVAIADIIMSLDNVIAIAAAAGGNMALLVIGLGISIPLIIAGAA
                     LIMSLLDRFPILVWAGAALLGWIVGEVIATDPVIATWLTSQYGAVIAHRVEIGAAAIG
                     AALVLLVGGLWRRSKLRSKLQPESET"
     gene            complement(3122..>3256)
                     /locus_tag="VFL49_10200"
     CDS             complement(3122..>3256)
                     /locus_tag="VFL49_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7912392.1"
                     /translation="KAADGTISKIHARSIGENSVHGSDAPDTAAREIAQFFSDNEIVG
                     "
BASE COUNT          633 a         1043 c          954 g          626 t
ORIGIN      
        1 taggaattgt attgtttgca attgccgtcc gaggcggcgg ccgatattcc gccgaccgta
       61 tgatagggaa agaactgtaa gatttcggca cgaaccgccc gcgcggcttc ccgtctagtc
      121 cgcgcgtgct ttgcgtatcc agaccttgtt gtcgtggacg gcggccccgc cataaggcgc
      181 aattgaatcc gcgccgataa gcgtgttgat gccgcgaccg tcggcatagg ccgcattcgg
      241 ccaaatcgac tcggcgatca gaacgcctcg ccgcacgccc ttgaacacgt gcgcgtgcag
      301 acgcacttcg ccgcgcttat tgccgagaac gacgtagtcg ccggtcgata tcccgagtgc
      361 cgcagcgtcg ccggggtgaa tcatcacggt cggccggttt tcctgcgcca aggatgtcgg
      421 cgtctcgttg aacgtcgaat tgagaaagcc ccgcgccggc gacgtcgcga gccgaaacgg
      481 atgctcggcg tccgccacct cgatcgacgt ccaatagtcg ggaagccccg gcatatcggc
      541 aatcgggcca ctcttgcagg ggctgcggaa cggaacgttg ggccaatccg gtttaaacct
      601 gaacttgccg tccgaccacg gaaaaccgtc aaggaaatgc gactcgcgga aaggcggctg
      661 gcagtcgatc cacttgttgg cctcgatttc ggcaagcgag ccgcgtttcg acacttgcag
      721 catctggtcg atgagctcgc gcgggctcat cgtaaaaccg cgatgctcgg catggagccg
      781 ctgcgcaagc ccgcagatca cctcatggtt gtttcggcac tcgccgggcg gctcgaccag
      841 cttcggtcca aggatgaggt actggtgccc gcccccacgg taaatatcat cgtgttcgag
      901 aaacgtcgtt gccgggagca ccacatcagc ggcttgcgcg gtctccgtca tgaactgctc
      961 gtgcacgcag acgaacagat cctcgcgcgc aaatccacgt ttcacctgcg tttgatcggg
     1021 gcaaacggac accggattgg tgttctggat aatcatcgcg gtgaccggcg cgccaccatg
     1081 cagcgcctca cgttcgccgg tcaggatggc gccaatgcga gattgatcga gcatccgcac
     1141 cgagtggtcc gagacatcaa gaccttcgat catggtcttg tcgagccgga aaatgtcagc
     1201 gttcatatgg aaggcaccgc cgccttcgtg ctgccaggca cccgtcaccg ccggaatgca
     1261 gcttgcggcg tgcatattcg ccgcgccgtt gcgcgagcgc gaaaacccgt aaccgaggcg
     1321 gaaaaaagcc cgcttgcgtt cgccgatcag ccgcgcgaac tcctcgatag tctcggccgg
     1381 tgcgccggtg atcgcagcgg cccattgcgg atcgcgtgag cgcagatgcg cttcgagctc
     1441 atgcggcgca tcggtgtact tttcgagata attccagtcg gctttgccgt cacggaacag
     1501 gcaatgcatc accgcgcaag cgagcgcgcc gtctgtgccc ggtttgatca gaaccggcag
     1561 atcagcctgc tccattgttc cgttcatata gatgtcgaca gccgcaatct ttgcgccgcg
     1621 ttccttccgc gcgcgcacgg catgggtcat gacattgacc tgggtattca ccgcgttggt
     1681 gccccagatg atcaccaaat cggactttgc catttcacgg ggatcgacgc cggcgatgcg
     1741 tccgattccg gcggcgtagc ccgcgtaggc aggattgacg cagatcgtcg agtgaaatcc
     1801 ggaatacttc ttgacgtgac gcaggcgatt gattccgtcg cgcatcacca gacccatcgt
     1861 gccggcgtaa tagtagggcc agacgctttc tgcgccatag cggcgctcgg cttcgatgaa
     1921 cttttcagcc acgagatcga gcgcgtcctc ccacgaaatg cgttcgaact ttcccgatcc
     1981 ttttgcgcca actcggcgca gcggatacaa caggcgctcg gcatgatgct cgcgctcggc
     2041 gtagcgcgcg accttggcgc agatcacgcc ggctgtgtag tcgttgtcct ttgagccgta
     2101 gacgcgtccg atcgtgcgct cgtcaagaat ctcgacctcc agcgcacagg tcgacgggca
     2161 gtcgtgcgga caggctgagt ggccgatccg caccgccttt gggagccgca ccgattgatt
     2221 catggctcct cattatagcc cattgcgggc taggtttcgc tctccggctg aagcttcgac
     2281 cgaagcttgg accggcgcca gagtccgcca accagaagca ccagcgcggc accgatcgct
     2341 gctgctccga tctcgaccct gtgcgctatc accgccccgt actggctggt cagccaggtc
     2401 gcgattaccg gatcggtggc gatgacctct cccacaatcc agccgagcaa agccgcacca
     2461 gcccaaacca ggatcgggaa ccgatccaac agcgacatga tcaaggcggc accggctata
     2521 atgagcggaa tgctgatacc gaggccgatg acaagcaacg ccatgttacc gccggcggca
     2581 gccgcaatcg cgatgacgtt gtcgaggctc atgatgatgt cggcaatcgc gacgatacgg
     2641 acggcgcgcc agagatgctc ggccgcctcc gcctcgtccc cgtccgcatc ttccggcacg
     2701 aggagttttg ccgcgatgta aaacagcgcg aggccgccga cgatcttgag gtagggcagc
     2761 aacatcagcg atgcgacgag gccggtaaaa ataattcgca atccaacggc aacgccggcc
     2821 cccaggatca tgccccagat gcgttgccgg tgcggcagcc cgcggcacgc catcgcaatg
     2881 acgacggcgt tgtcgcccga cagcaggatg ttgatccaca tgatcttggc cagcgcgatc
     2941 cagaattccg catggcccat gtcgttccaa ccggaactga ccatcgccca ccaacttgcc
     3001 ggatccatga gctgcgacat cgaacccact gacgtttcct cccccaattc ccgcgttctt
     3061 atgctcgcgg tttatcccgc tttcggcaac cgaagacttg gcccccagat cgactgacgg
     3121 cctagccgac gatttcgttg tccgaaaaga attgcgcaat ttcccgcgcc gcagtgtccg
     3181 gcgcgtccga gccgtgaacg gagttttccc cgattgacct ggcgtggatt ttactgatcg
     3241 tcccgtcagc cgcctt
//
LOCUS       DASSRL010000501         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1431088, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000501 DASSRL010000000
VERSION     DASSRL010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431088"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2848)
                     /locus_tag="VFL49_10205"
     CDS             complement(<1..2848)
                     /locus_tag="VFL49_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase/phage tail family protein"
                     /protein_id="HET7912393.1"
                     /translation="MAALVLSTAGAAAGKAVFGPAGAVAGRLIGALAGNAIDHALFGG
                     RQEIAQEGPRLADLAVMASTEGAPIPRVYGRARLPGQVIWATNLEEVVGTTSQTTGGG
                     GGKGMGGSPATTTSTTTYSYFANLAIGLCEGPIGAVLRIWADGKPLDLTNLSIRVYTG
                     DETQAPDPLIVAKEGDVPAYRGLAYVVFERLPLANFGNRIPQLSFEVVRPVGRLEQML
                     RAVTLIPGTTEFGYAPATVVQTLGPGQSAPENRHVAYARSDVVAALDELQAVAPNIER
                     VAIVVAWFGTDLRCGHCRIMPGVDNRIKETFGGKWSVTGLDRAAAHLVSTVDGKPAFG
                     GTPSDQSIRDLIAEIKSRGIKVTLYPFLMLDIPAANTLPDPLSGAPSQPAYPWRGRIT
                     CDPAPGQAGSPDGSAAAAAQINTFFTGGTDERNYRNFVMHYASLAASAGGVDAFLIGS
                     ELKSLTRVRSASGVYPAVAALTALAADVKAIVGAGTVVTYGADWTEYGAHVVDGEASE
                     IRFPLDALWASPSIDVIGIDYYAPLSDWRDAADHLDLAQNGNPYRAGYLARNLAAGEA
                     YDWFYADEAARDAQARTPITDGLGKPWVFRQKDLWSFWSQSHYERVDGTELANPTAWV
                     PQSKPIWLTEIGCPAVDKGANQPSVFPDPKSSEAGLPHYSSGDRDDLIQRRYIEGALG
                     AFDPAFGEAPLNPVSAIYGGRMVAPDGVHVWTWDARPFPAFPALIDTWNDAANWETGH
                     WLTGRLGSTPLDGLVSAILSDSGISATDTSELGKGADGYVVDRPMTPRAMLDPLALTF
                     AFDAFEQDGVLRFRQRGGLPVAELTEDDLVLSDEGAPALLVRGQESELPREVTLAFTD
                     IGADYARAAAASRRLVGGSARSAHADLAVVTNDGEATRRAEIWLQDLWAGRESASFAL
                     PWSKLGLAAGDIIALTVNTRRQLLELQALSDAESR"
     gene            complement(2848..3285)
                     /locus_tag="VFL49_10210"
     CDS             complement(2848..3285)
                     /locus_tag="VFL49_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET7912394.1"
                     /translation="MLVHRNDIVAEARRWIGTSYRHQASLRGVGCDCLGLVRGVWRAV
                     IGDEPEHAPPYAPDWAEATGEESLAEAATRHLIAIPRDGFAPGDVLLFRWRAHLPAKH
                     AAIVTTSGTMVHAHDGAAVAEVAIAPWWRRRLAFAFQFPGVIG"
     gene            complement(3288..4175)
                     /locus_tag="VFL49_10215"
     CDS             complement(3288..4175)
                     /locus_tag="VFL49_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HET7912395.1"
                     /translation="MRPVPSPMQLKLNSGATTLCRCWVIRRADGITQGFTDHDEDVAL
                     DAITCRAGSGLTGSEATQNLGFAIDSSEFSGALADDALNEADLAAGRYDAAVAEIWLV
                     DWSEPALRVLLAKGTLGEVRREGRAFTAEVRGLSDRLSQQSGRLYTATCSADLGDDRC
                     TIDLTDPTFRGVGAVASADATSAFTASGLDGFEDGWFTAGKLTFNSGANIGLSVEVKS
                     HRKGSAVKLVLWQAMPELIATGDTFIVTAGCDKRFETCRGRFSNAVNFRGFPHIPGND
                     FIIAHPLPGEPGHNGASRR"
     gene            complement(4172..>4272)
                     /locus_tag="VFL49_10220"
     CDS             complement(4172..>4272)
                     /locus_tag="VFL49_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HET7912396.1"
                     /translation="VPVRFDTDYLEVDLSAFAAGAIPKIPLVEIRP"
BASE COUNT          794 a         1378 c         1321 g          779 t
ORIGIN      
        1 tgcggctttc ggcgtcgctt aacgcctgca gttccaagag ctgacgccgc gtattcaccg
       61 tcagggcgat gatatcgccg gccgccagtc cgagtttgct ccaaggcaat gcaaagctgg
      121 cactttcgcg gccggcccag agatcctgca gccagatttc cgcgcggcgg gtcgcctctc
      181 catcatttgt gacgactgca aggtccgcat gggcgctgcg ggctgatccg ccgaccaggc
      241 ggcgcgacgc agccgcggcg cgcgcgtaat cggcgccaat atcggtgaaa gcgagtgtta
      301 cttcccgtgg aagttcactt tcctgtccgc gcaccaggag tgcgggcgcg ccctcgtcgg
      361 acaataccag gtcgtcttcg gtcaattcgg caaccggcaa gccgccgcgc tggcggaagc
      421 ggagcacgcc atcctgctca aatgcgtcga atgcgaacgt gagcgccaat ggatccagca
      481 tcgcgcgcgg tgtcatcggc ctgtcgacaa cgtacccgtc ggcgcccttg ccgagttcgc
      541 tggtatccgt tgcgctgatg ccgctgtcgc ttaaaatcgc cgagacaagg ccgtcgagcg
      601 gggtcgatcc gagccgccct gtgagccaat gccccgtctc ccagttcgcc gcatcattcc
      661 atgtgtcgat cagcgccggg aacgccggaa atggccgcgc atcccaggtc catacgtgga
      721 cgccgtctgg cgcaaccatc cgaccgccat agatcgcgga caccggattg agcggcgctt
      781 cgccgaatgc agggtcgaaa gcgccgagcg caccctcgat gtagcggcgc tggatcaggt
      841 cgtcccggtc gccgctggaa taatgcggca ggcccgcttc cgacgatttc gggtcgggaa
      901 aaacgcttgg ctggttcgca cccttgtcga cggcaggaca gccgatttca gtaagccaga
      961 tcggttttga ctgcggaacc caggccgtcg ggtttgcaag ttcggtcccg tcgacacgct
     1021 catagtgtga ctgcgaccag aagctccaca gatctttctg tcggaagacc caaggcttgc
     1081 cgagaccgtc cgtgatcgga gttcgagcct gtgcgtcacg tgcggcttcg tcggcgtaga
     1141 accagtcata ggcctcgcca gctgcaagat tgcgggcaag atagccggcc ctgtacgggt
     1201 ttccgttttg cgccaagtcg agatgatccg ctgcgtcccg ccaatccgac agcggcgcgt
     1261 aataatcgat gccgatcacg tcgatggaag gtgacgccca caacgcatcg agcggaaatc
     1321 gaatttcgct tgcctctcca tcgacgacgt gcgcgccata ttcggtccaa tcggcgccat
     1381 aggtcacaac tgtgccggcg ccgacgattg cctttacgtc ggcggcaagt gcggtgagcg
     1441 cagccaccgc tggatagaca ccggacgccg atcgaacgcg tgtgagcgac ttcagctccg
     1501 agccgatcag gaaagcgtcc acgccacccg cgcttgctgc gagcgatgcg tagtgcatta
     1561 cgaagttgcg gtaattccgc tcatcggtgc cgcccgtgaa gaacgtattg atctgggccg
     1621 ctgctgctgc gctcccatcg ggtgatccgg cctgtcccgg cgctggatcg caggtgatgc
     1681 gtccgcgcca tggatatgct ggttgcgacg gtgcgccgct caaaggatcg ggcaacgtgt
     1741 ttgctgccgg aatatccagc atcaaaaagg gatagagcgt gaccttgatc ccacgacttt
     1801 tgatttccgc gatcaggtcg cggatgctct gatcggacgg cgtgccgccg aaggcaggct
     1861 tgccgtcgac cgtcgatacg aggtgcgccg ccgcccggtc caggccggta acagaccatt
     1921 tgccgccgaa ggtttctttt atgcgattgt cgacgcccgg cattatccgg caatgaccgc
     1981 agcgcagatc ggtgccaaac cacgcgacca cgatcgccac gcgttctatg ttcggcgcca
     2041 ccgcctgcaa ctcgtcgagg gcggcgacga cgtcggaccg cgcgtaggca acatgacggt
     2101 tttccggagc cgattggccc ggtcccagtg tctgcaccac ggtcgccggc gcgtagccga
     2161 attcggtcgt ccccgggata agcgttacgg cacggagcat ctgctcaagc cggccaactg
     2221 gacgcaccac ctcaaacgac agttgcggga tgcggttgcc gaaatttgca agcggcaaac
     2281 gctcgaacac aacgtaggcg aggccgcgat aggccggcac atcgccttcc ttggcgacaa
     2341 tcagcgggtc gggcgcctgc gtttcatcac cggtataaac gcggatggaa aggttcgtga
     2401 gatcgagtgg cttgccgtcg gcccagatgc gcagcaccgc accgatcggc ccttcacaaa
     2461 gaccgatcgc gagattggcg aaatatgagt acgtcgtcgt gcttgtcgtg gtggcgggac
     2521 ttccgcccat gccctttccg ccgccgccac ccgttgtctg cgacgtggtg ccgaccacct
     2581 cctcgagatt tgtcgcccag atcacctggc ctggaagacg cgcccggccg tagacgcgcg
     2641 ggattggggc accctcggtg gaggccatca ctgcaagatc ggctagccgc gggccctcct
     2701 gcgcgatctc ctgccggcca ccgaacaagg catgatcgat cgcattgccg gccagtgcgc
     2761 caatcaggcg gccggcaacg gcgccggccg gcccgaaaac cgctttgcct gcggcggcgc
     2821 cggcagttga aagaacaagc gcggccatca gccgattact ccgggaaatt gaaatgcgaa
     2881 cgcgaggcgc ctgcgccacc atggcgcgat ggcgacttcc gcaacagccg cgccgtcgtg
     2941 cgcgtgcacc atcgtgccgg acgttgttac gattgccgca tgttttgccg gcagatgcgc
     3001 gcgccagcgg aacaggagca catcgcctgg cgcgaatccg tcgcgcggaa ttgcaataag
     3061 atggcgtgtc gccgcctcgg caagcgactc ttcacccgtc gcctccgccc aatcgggcgc
     3121 ataaggcggt gcgtgttccg gctcatcgcc gatcacggcg cgccagacgc cgcgcacgag
     3181 accgaggcaa tcgcaaccga caccgcgcag cgaggcctga tgacgatagg acgtgccgat
     3241 ccagcgccgc gcttccgcga cgatatcatt tcgatggaca agcatgttta ccggcggctg
     3301 gcgccattgt ggccgggctc gcccggcagc ggatgcgcga tgataaagtc gttacccggg
     3361 atatgcggga atccccggaa attcacggcg ttactgaatc ggccacgaca ggtttcgaag
     3421 cgcttatcgc aaccggcggt cacgatgaac gtgtctccag ttgcgatcag ctccggcatc
     3481 gcctgccaca gcacgagttt gacagcggaa cccttgcggt ggctcttcac ttccacactc
     3541 aggccaatgt ttgctccgct gttgaaagtc agttttccgg cggtgaacca gccatcctcg
     3601 aaaccgtcga gcccgcttgc cgtgaacgcc gaggtcgcgt ccgccgacgc cacggctccg
     3661 actccacgga aagtcgggtc ggtgagatcg atcgtgcagc ggtcatcgcc gagatcggca
     3721 gagcatgttg ccgtatatag ccggccgctc tgctgcgaca gccggtcgct caatcctcgc
     3781 acctctgcgg tgaacgcccg accctctcgc ctcacctcgc caagcgtgcc tttcgcgagc
     3841 aatacccgca gcgccggctc actccaatcg accagccaga tttcagccac agccgcatcg
     3901 tagcggccgg cagccagatc ggcttcgttg agcgcgtcgt cggcgagcgc gccggagaat
     3961 tcggaagaat cgatggcaaa tccaagattc tgcgttgctt cgctgccggt caggccgctg
     4021 cccgctcggc atgtgatggc gtcgagcgca acatcttcgt catggtctgt gaatccctga
     4081 gtgatgccgt cggcgcgcct gatgacccaa caccggcaca gagtggtggc acccgaattg
     4141 agttttaatt gcatgggtga cggaaccggt ctcacggcct gatctccaca agtgggattt
     4201 tcgggatagc gcccgctgca aaggcggaaa gatcgacttc gagatagtcg gtgtcgaaac
     4261 gcaccggcac gt
//
LOCUS       DASSRL010000502         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_765728, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000502 DASSRL010000000
VERSION     DASSRL010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765728"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFL49_10225"
     CDS             <1..175
                     /locus_tag="VFL49_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495540.1"
                     /note="FabF, beta-Ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HET7912397.1"
                     /translation="LQSQRIPPTINYTVPDPSIPLDVVPNRARDASLRHAISNSFGFG
                     GQNVSLVMGLEPA"
     gene            196..1215
                     /locus_tag="VFL49_10230"
     CDS             196..1215
                     /locus_tag="VFL49_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7912398.1"
                     /translation="MRALRLIADRKLELADAPPPPPPGDGEVQIRIKAVALNHLDLWG
                     FRGMAFAKRQLPLVVGAEASGEIAAVGKGASFRPGDRVVMYGAMTCGHCAACRDGRDN
                     LCENVAGIMGFHIDGFARDLINLPARLAIPVPDGVSLRDAACAPIAFGTVEHMLFDNA
                     KLKPGETILVHAGGSGIGSVAIKMAKALGCTVITTVGDDSKAEKAKALGADHVINYKT
                     ERFEGVTRKLTGKKGVDVVFEHVGGEAFNGSLLCLKRGGRLVTCGSTAGPTVTINLMQ
                     LFQQQYKIFGSFGASMRNIRDSLSKIAAGMTPIIDTEVDLADFERGLARLESRQVFGK
                     VIVTF"
     gene            1226..2182
                     /locus_tag="VFL49_10235"
     CDS             1226..2182
                     /locus_tag="VFL49_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HET7912399.1"
                     /translation="MRRIVRRLKRSSKRVANSAVAAVTIGLLKLLRLTNPDTMGNVAG
                     WFMRTVGPLLPENRIGRANLTMAFPEKSPAEIDTILRGVWDNLGRIAAEFAHLDRLSD
                     FDPWHPERPARIVARQSDIERFFKLIEQRRPAIVFAAHFANWELPAVCAAACKLDTAV
                     LYRRPNNAAIDKWLHDTRAAAMGMLIPTTLDAPVKLADALERGTHIGMLVDQYYSRGV
                     DVTFFGQLTKANPLLARLARHFDSPIHGLRVIRLPGHRFRAELTEQVQPIRDAEGKVD
                     IAATTQAVMSVIEGWIREYPEQWLWLHRRWRPEDQDSINARS"
     gene            complement(2166..>3185)
                     /locus_tag="VFL49_10240"
     CDS             complement(2166..>3185)
                     /locus_tag="VFL49_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7912400.1"
                     /translation="VVNVYNWSDYIDPKVLEEFTKETGIKVQYDTFDANETLETKLLA
                     GKSGYDVVVPTGYFLERQIKAGVFQKLDKSKLKNLVNVWPEIASRLAAYDPGNQYAVN
                     YMWGTTGIGFNVKAVRDRLGRDGKIDSWDIVFKPDNLAKFKDCGIHMLDSADDIIPAA
                     LHFLGLDPNSSDPTDLEKASELLAKIRPLVRKFHSSEYLNALASGEICLVVGWSGDVK
                     QAQKRAAETKNGVEVGYSIPKEGAQMFFDNLAIPKDAAHVAEAHAFIDYLLRPKIAAL
                     NSNLVSYANGNLASRQFIDRSILDDKSIYPDASTMSLLYTVGARDQKSQRVINRVWTK
                     IKTGR"
BASE COUNT          642 a          948 c          912 g          683 t
ORIGIN      
        1 tttgcaaagt cagcgtattc cacccaccat caattatacg gtgcccgatc cctcaattcc
       61 gctcgacgtc gtgcctaaca gggcgcgcga cgccagcttg cgccacgcga tttcgaactc
      121 atttgggttt ggcggacaaa acgtttcgct ggtgatgggg cttgagccgg cctagtgccg
      181 gacatgccga gccccatgcg tgcgctaaga ctgatcgccg accgtaagct cgagcttgcc
      241 gatgctccgc cgcctccacc accgggcgac ggagaggtgc aaatccgcat caaggcggtg
      301 gcactcaatc atcttgatct ttggggcttc cgcggaatgg cgttcgccaa gcgtcaactc
      361 cccttggttg tcggcgctga ggcttcgggt gaaattgcgg ctgttgggaa aggcgcgagc
      421 ttcaggccgg gcgacagggt cgtgatgtat ggggccatga cgtgcggcca ctgcgcggct
      481 tgccgcgatg gccgcgacaa tctctgcgaa aacgtcgccg gcatcatggg tttccacatc
      541 gacggcttcg cgcgcgatct gatcaattta ccggcccggc tcgccattcc ggtccccgac
      601 ggcgtgtctt tacgcgacgc agcgtgcgca ccgatcgcct tcggcaccgt cgagcacatg
      661 ctgttcgata acgccaaact caagcccggt gaaaccatat tggtgcatgc cggcggctcc
      721 ggcatcgggt cagtggcaat caagatggcg aaggcgcttg gctgcacggt gatcacgaca
      781 gtcggcgatg actcgaaagc ggagaaagcc aaggcactcg gcgccgatca tgtgatcaac
      841 tacaagaccg aacgctttga aggcgtcaca cgcaagctga ccgggaaaaa aggcgtcgac
      901 gtcgttttcg agcatgttgg cggggaagcc ttcaacggct ccctgctctg cctcaagcga
      961 ggcggccgcc tggtgacctg cggttcaacc gccgggccaa cggtgacaat caacctgatg
     1021 cagctgtttc agcagcaata caagatattt ggttccttcg gcgcttcaat gcgcaatatt
     1081 cgcgacagcc tgtcgaaaat cgctgccgga atgaccccga tcattgacac ggaagttgat
     1141 ctcgccgatt tcgaacgtgg cctggcgcgc cttgaaagcc gccaggtgtt tggcaaagtc
     1201 attgtgacgt tttgaggcgt cagcgatgag acggatagtc cggcgcctga agcgctcaag
     1261 caaacgtgtc gccaattccg cagtcgccgc cgttacgatt ggccttctga aactgttgcg
     1321 tctgaccaat cccgacacaa tgggaaacgt cgcgggctgg ttcatgcgaa ccgtgggacc
     1381 cttgctgccc gaaaatcgca tcggacgggc caatttgacc atggcattcc cggaaaagtc
     1441 accggccgag atcgacacga tcctgcgcgg cgtttgggac aaccttggcc gtattgccgc
     1501 tgaatttgcc cacctcgacc gtctgtccga cttcgatccc tggcatcccg aacggcctgc
     1561 acgcatcgta gcaaggcagt ccgacatcga gcgattcttc aagctgattg agcagcgccg
     1621 gcctgcgatc gtattcgccg cgcatttcgc caattgggag ctgccggcag tctgcgcagc
     1681 ggcttgcaag ctcgatactg ccgtcctcta ccggcggccg aacaatgccg ccatcgacaa
     1741 atggctgcac gacacgcggg ccgcggcgat gggaatgctc attccgacaa cgctcgacgc
     1801 gcccgtgaaa ctggcggatg cgctcgaacg gggcactcac atcggcatgc ttgtcgatca
     1861 atactacagc cgcggcgtcg acgtgacctt ctttggacag ctcaccaagg cgaatcccct
     1921 cctggcgcgg cttgcccggc attttgatag cccgattcac ggcctgcgcg tcatacgcct
     1981 tccgggccat cgttttcgcg ccgagttgac cgagcaggta cagcccatcc gtgatgcgga
     2041 aggcaaggtt gatatcgcgg caaccacgca agccgtcatg tcggtgatcg agggctggat
     2101 tcgcgaatac cccgagcagt ggctttggtt gcaccggcgc tggcggccgg aggatcagga
     2161 ctcgatcaac gcccggtctt gattttggtc cacacccgat tgatcacacg ctgcgatttc
     2221 tgatcacgcg cacctaccgt atacagaaga ctcatggtcg aagcatccgg atagatgctc
     2281 ttgtcgtcga ggatcgaccg gtcgatgaat tgccggctgg cgaggttccc gttggcgtac
     2341 gaaacgagat tgctgttcaa cgccgcaatc ttcggcctga gcaagtagtc aataaacgca
     2401 tgcgcttctg caacgtgcgc ggcgtctttc ggaatggcga ggttgtcgaa aaacatctgc
     2461 gcgccttcct tcggaattga atagccgact tcgacgccgt tcttcgtttc ggccgcgcgc
     2521 ttttgtgcct gcttgacgtc gcctgaccaa ccaaccacca gacagatttc gccgcttgcc
     2581 aaagcgttca ggtactcgga cgaatgaaat ttccggacca ggggacggat tttcgcgagc
     2641 agttcgctcg ccttttcgag atcagttgga tcactcgaat tcggatcgag cccgagaaaa
     2701 tgcagcgctg ccggaatgat gtcatccgcc gaatccagca tatggatacc gcaatccttg
     2761 aatttggcga gattgtccgg cttgaagaca atatcccagc tgtcgatttt gccgtcccgg
     2821 ccaagacggt cgcgcaccgc tttcacgttg aacccgatac cggtcgtgcc ccacatataa
     2881 ttcacggcgt actgattacc gggatcgtaa gccgcgaggc ggcttgcgat ttccggccat
     2941 acgttcacga gattcttcag tttgcttttg tcgagcttct ggaaaacgcc ggccttgatt
     3001 tgtcgctcga ggaaatatcc tgtcgggacc acgacgtcgt aacccgattt gccggcgaga
     3061 agcttggttt cgagcgtctc attggcatcg aaggtgtcat attgcacctt tatgccggtt
     3121 tccttggtga attcctcgag aactttcgga tcgatatagt ccgaccagtt gtagacatta
     3181 accac
//
LOCUS       DASSRL010000503         4219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1745753, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000503 DASSRL010000000
VERSION     DASSRL010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745753"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFL49_10245"
     CDS             <1..603
                     /locus_tag="VFL49_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7912401.1"
                     /translation="EFESGPRLTARDMVTTVNELTDGWTYDVVSIGYPGPVAGNRPVA
                     EPYNLGSGWAGFDFGKAFHRPVKVVNDAVMQALGSYRGGRMLFLGLGTGLGSAMIIDG
                     AIEPMEIAHLPYKKGKSFEDYAGTRGLERLGLKKWHRHVADIVRILSAALEPDYVVLG
                     GGNVKKIRKLPPRTRRGSNSNAFGGGIRLWKGHRAHVRAN"
     gene            587..1351
                     /gene="pgl"
                     /locus_tag="VFL49_10250"
     CDS             587..1351
                     /gene="pgl"
                     /locus_tag="VFL49_10250"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET7912402.1"
                     /translation="MSGPTERPGEWRLFETAEAVAEHAAEWLCLCVLARPRDGEVAIC
                     LSGGSTPQRLYQRLASAPVASRMPWNRIHWFWGDERFVAHDDPASNYRMAHEALFSRA
                     PVSPDHIHPIPTEGLTPEQAAAAYQSVLQTFYGSTRPEVIRPVFDVTLLGVGEDGHTA
                     SLFPGSSALQEERQWVLPVLADASRTRVTLTYPVLNSSRNLAFLATGTNKKKILTRIR
                     TGDTAPPAARIRPIGRLTWFVDRAAMPDWPPAASSN"
     gene            complement(1416..2309)
                     /locus_tag="VFL49_10255"
     CDS             complement(1416..2309)
                     /locus_tag="VFL49_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7912403.1"
                     /translation="MKKSLVALVLLAAAALGGVAFRVLTTPAVPQIALASYNPDLANG
                     RTMFLIGGCSSCHAVPKQDDATRLGGGLALPTAFGTFHVPNISSDAKDGIGGWSEAQF
                     IAAMRFGISPSGEHLYPAFPYTSYHAMRTEDLRDLFAYLRSLPAIPGTTPAHALKFPF
                     NIRRAVGLWKLLFLDDAQFKPDASQSTQWNRGAYLVNGPGHCAECHSPRNFLGAIIAG
                     RRFTGGQSPTGQGGVPDLTQGKLHEWKVEDFVEVLDSGMTPDADRVGAAMVDVVANTR
                     QLGAADREAIAVYLKSLRAVK"
     gene            complement(2404..2853)
                     /locus_tag="VFL49_10260"
     CDS             complement(2404..2853)
                     /locus_tag="VFL49_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7912404.1"
                     /translation="MGRPAGAALAAAILVLGAGAVIAQSNPIATRMALMKGNNDNAKI
                     VVGMMRGQVPFDAAKVEAAFAQWADTAQKLPSLFPDNSKQGEKTRAAPKIWMTKADFD
                     AKAVDFGRLVAENRDKARSSVDGLRAAIPAVANACDNCHKDYRLSQQ"
     gene            complement(2921..3256)
                     /locus_tag="VFL49_10265"
     CDS             complement(2921..3256)
                     /locus_tag="VFL49_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912405.1"
                     /translation="MSARAKKSVFRRAGHQASDGDAAVFQVGAQGEGKRIEKGKVLSY
                     DLGNRIDILGALPAERVMGAPALGHLCARFAKGYGVALAETIALNDGGSPSRCSRFCD
                     RDRRGRRPE"
     gene            3955..>4219
                     /locus_tag="VFL49_10270"
     CDS             3955..>4219
                     /locus_tag="VFL49_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912406.1"
                     /translation="MAPGFVEQATEDHLPTLSPDKQKAEIAIRRADGRGPSRALSTFV
                     FEKLNIAQRLLDENKGKHLLEFTIAPADILHRADLRIYDEIVRA"
BASE COUNT          815 a         1297 c         1298 g          809 t
ORIGIN      
        1 gaatttgaat ccggtccgag actcacggcc agggacatgg tcacaacagt caacgagttg
       61 acggacggct ggacttacga tgtcgtttcg atcggctatc ccggcccggt ggccggcaac
      121 cggcccgtcg cggaacccta caatcttgga tcaggctggg caggcttcga cttcgggaaa
      181 gcctttcatc gtccggtgaa ggtcgtcaac gatgccgtca tgcaggcgct tggcagttat
      241 cgcggcggtc gcatgttgtt tctcggtctc ggcaccggat tggggtcggc aatgattatt
      301 gacggtgcga tcgagcccat ggagatcgct cacctgccgt acaaaaaggg aaagagcttc
      361 gaagactatg ccggcacgcg cgggcttgag cgtcttggat tgaaaaaatg gcacaggcac
      421 gtggccgaca tagtcaggat tttgagcgcg gcgctcgaac cggactatgt cgtgcttggc
      481 gggggtaacg taaaaaaaat aaggaaattg ccgccccgta cgcggcgcgg cagcaacagc
      541 aacgcgtttg gcggcggcat ccgtctctgg aagggccatc gcgctcatgt ccgggccaac
      601 tgaaagaccg ggcgaatggc ggctctttga aacagccgaa gcggtcgcag agcacgctgc
      661 cgaatggctg tgcctgtgcg tgctcgcacg cccgcgcgat ggtgaggttg caatctgcct
      721 gtccggggga tcaacgccac agcgccttta tcagcggctc gcgagcgctc cggtcgcttc
      781 gcgcatgccg tggaatcgga tccattggtt ctggggcgac gaacgcttcg tcgcgcacga
      841 tgatcccgcc agcaactacc gaatggcgca tgaggctctt ttttcacgcg caccggtctc
      901 accggaccac attcatccaa tcccgacaga gggcctgacc ccggagcagg ctgcggcagc
      961 gtaccagtca gtgctgcaaa ccttctacgg cagcacgcgg cccgaggtga ttcgacccgt
     1021 cttcgacgtg acattgttgg gtgtcggcga ggacgggcac acggcctcgc tttttccggg
     1081 cagttcagcg ctgcaagagg aacggcaatg ggttcttccg gttctcgccg acgcaagtcg
     1141 gacccgcgtc accctcacct atccggttct caacagcagc cgcaatctcg ccttcctcgc
     1201 gacgggcacc aacaagaaaa aaatcctgac acgcattcga accggggata ctgcgccgcc
     1261 ggccgcccga attcgcccca tcggacgtct cacctggttc gtggaccgcg ccgcaatgcc
     1321 tgactggccg ccggcggcca gttcgaactg accgtccaaa cgggctgatt tcgatggaat
     1381 atgacgcggt gccgggcaga tgaccggcgc tgccgctact tcactgcgcg cagcgacttc
     1441 aggtagacgg cgatggcttc gcggtcggcg gcgccgagct ggcgtgtgtt ggcgacgaca
     1501 tcgaccatcg cagcgccgac acggtcggca tcgggcgtca tgccgctgtc gagcacctcg
     1561 acaaagtcct cgaccttcca ttcgtgcagt ttgccctgcg tgagatcggg aacgccgccc
     1621 tgacccgtcg ggctctggcc gccggtgaag cggcggccgg cgatgatcgc gccgagaaaa
     1681 ttgcgcggcg aatgacattc ggcgcaatgg ccgggcccgt tcaccagata ggcgcccctg
     1741 ttccactgcg tggattgcga agcgtccggc ttgaactgtg cgtcatcgag aaacagcagc
     1801 ttccataaac cgacggcgcg gcggatattg aaaggaaatt tcagggcgtg cgcgggcgtg
     1861 gtgccgggaa tagccggcag gctcctgaga taggcgaaca gatcgcgcag gtcttccgtc
     1921 cgcatcgcat gataggacgt gtagggaaat gccgggtaga ggtgctcccc cgacggggaa
     1981 atgccaaatc gcatggcggc gatgaactgg gcctcgctcc agccgccgat tccatctttg
     2041 gcatcagacg aaatgttggg aacgtggaac gtgccaaagg cggtcggcag cgcgaggccg
     2101 ccgccgagcc gcgtcgcatc gtcctgcttg gggaccgcgt ggcacgagga gcagccgccg
     2161 atgagaaaca tcgtgcggcc gttggccagg tcgggattgt aggacgccag cgcgatctgc
     2221 ggcacggcgg gcgtcgtcag cacccggaat gccacgccgc ccagcgcggc ggcggcaagc
     2281 agcaccagtg caaccagact cttcttcatc gcattgcccg cgctggcccg gcaccgtcat
     2341 aaacaaattg atatgccggc gcgacccagt cacgccggca cggtgaacaa atacgataga
     2401 tcgctattgc tgcgacaggc gataatcctt gtggcaattg tcgcaggcat tggcgaccgc
     2461 cggaatagcc gcgcgcagac cgtcgacaga gctcctcgcc ttgtcgcgat tttcagcgac
     2521 gagcctgccg aagtcgaccg ccttggcatc gaagtcggct ttcgtcatcc agatcttcgg
     2581 ggcggcacgc gtcttttcac cttgcttcga gttgtccgga aacagggacg gcagcttctg
     2641 ggcggtatcg gcccattgcg cgaaggcggc ctcgactttg gcggcgtcga agggcacctg
     2701 cccgcgcatc atgcccacga caatttttgc attgtcgttg ttccctttca tcagcgccat
     2761 gcgcgtcgca atcgggttgg attgtgcgat caccgcgccc gctccgagca caagaattgc
     2821 tgcggcgagt gccgctccgg caggtcgtcc cattggctaa tctcgctttg tttgtcctgg
     2881 ctgcccattc cccgtcagac aaacatctgg cccacaaagg ttattccggc cgccttcctc
     2941 ggcgatcgcg atcacaaaat cgtgaacatc ggcttggcga gccgccgtcg ttcaaggcga
     3001 tcgtctccgc aagcgccacc ccatagccct tcgcaaagcg agcacaaaga tgtccgagcg
     3061 caggagcacc catgacccgc tcagccggaa gggcgccgag tatgtcgatc ctattaccga
     3121 gatcgtagct caagaccttt cctttctcga ttcgttttcc ttcgccttgc gcgccgacct
     3181 gaaagacagc ggcgtcaccg tcacttgcct gatgcccggc gcgacggaaa accgatttct
     3241 tcgcgcgagc cgacatgctc gatacgaaag tcggcaccgg ggaagaagat ggatcccgcg
     3301 gaagtcgcca ggcagggctt taaggtgata ctcgatgacg aaggaggtgc cgtaacgggc
     3361 cggcagaaca agcggcggtc agccacttca ctgcttatgc ccgccggcat agcggcatag
     3421 caacattcgc aaaatggcgg agcctggcac cgctcaacga tgtacgacgg ccggatttac
     3481 ctcatttccg tcggcgccgg gcgcctgccc gacgctttcg attgtacgga gaccggttgg
     3541 ccggacgaag agaaattcct tcgcgatgtg ccttgactct gacggaatga gcggtccggc
     3601 ccagtttgag accgatcacg ccggtcggag tattggcacg cgcgaggctc ctgagttgag
     3661 cgaggtcgcg agcactccag tccttattac tgtttcgctt gtactgtgga tttgccttgc
     3721 gcggccccct gcggccgata ggtgatcccg gcctgcgcca gcttgctcgt gccgatttgg
     3781 gcatggtcct tgtctccgcg tctgactatc tctgccaaaa caatatggct gctgcagaaa
     3841 ggttccgcgc gaacgttccg ccgtccaggg agttctcttt cggcgcggcg cttgcgacgt
     3901 tcaactgtca tgtcgaatgg ggcaatcaaa ggctaccggt tctgcgagaa ggagttggcg
     3961 cccggattcg tcgagcaggc gacggaagac catctcccca cgctcagccc cgacaagcag
     4021 aaggccgaga ttgcgattcg ccgcgccgac gggcgagggc cttcgcgcgc tttatcaacg
     4081 tttgtctttg aaaaactgaa tattgcccaa cgcctgttgg atgaaaacaa aggcaagcac
     4141 ctcttggagt tcaccatcgc tcccgccgat attttgcacc gcgctgactt gcgcatctat
     4201 gacgagatcg tgcgagcca
//
LOCUS       DASSRL010000504         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1238276, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000504 DASSRL010000000
VERSION     DASSRL010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238276"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="VFL49_10275"
     CDS             complement(<1..2087)
                     /locus_tag="VFL49_10275"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET7912407.1"
                     /translation="MQTTLLGLAIAIILALVAALVGPLLLDWGNHRTLFEAEASRLIG
                     VNVRVTGGIDARLLPSPRLTLHDIQIGDGGNAVHARSLGVEFALGSLMRGEWRATELH
                     LVGPQVNLGLDSSGRLRAPNLAMTFNPDELSIDRLSIEDGTVVLADAASNTSLTLGRV
                     WFNGEARSLVGPMKGEGAVTIAGALYPYRLAFGRISDGGAVRLHLNVDPTDYPLNFEA
                     DGALAVTGGAPGFDGTFGLRRPVGIGLRRPGPAAGDVTQPWRVNGKIKATAQSALMQD
                     IEFQYGSEEQGVRLTGVADCKFGARPRLNAVLSGRQIDVDRAVSGGGSEKQTPTAVIR
                     QLTALAASAFRAPLPIQLGIGIDQVTLGGNGLQNLRGDISGNANGWSLDRLEFRAPGL
                     TQVRLSGRLDVGSDGVAFTGPAQIESSDPRALALWLEGRTETGQNDLRPLSLHGDITL
                     ASDRIAVDGLKAEFDRKPVTGRLAYTLASGDRPAKLDAELKAPQLDIDAAVAFGRGLL
                     AGSSFERPREMTLAIEVGRISFSGVDARDARVHLQVDPNGLQLDRLIVGDIGGGSFAA
                     TGRIETKGHVPRGSLVVDLETTKAAAIAAIAEKVVPDRVASGVRALERVGRAKLHATL
                     DVAGDDRTSATSAQVAVAGTLDELRLDARARIDGDWQTRSVSAVEMNATVDAATAASL
                     LKFANLDGLVASG"
     gene            complement(2207..>2540)
                     /locus_tag="VFL49_10280"
     CDS             complement(2207..>2540)
                     /locus_tag="VFL49_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7912408.1"
                     /translation="CVTETQRDIEASRIMAPIVGHVGDGNFHLTLLVDMNDADEIRRA
                     KALSERLVERALAMDGTCTGEHGVGQGKMKYLVAEHGAAALAVMASIKRALDPQNIMN
                     PGKIVALS"
BASE COUNT          457 a          837 c          769 g          477 t
ORIGIN      
        1 ttgcctgatg cgaccaatcc gtcgaggttc gcgaatttca gcaaagacgc cgcggtcgcc
       61 gcatcgacag tagcattcat ctcgaccgcg gatacgctgc gggtttgcca gtcgccgtcg
      121 atgcgtgcgc gcgcatcaag ccgcaactcg tcgagcgtcc ccgctaccgc cacctgcgcg
      181 ctggtcgccg atgttctgtc atcacctgcg acatccagcg tggcgtgaag ttttgcgcgt
      241 ccgacgcgct cgagagctct tacaccggat gcgacccggt cgggcacgac cttttccgcg
      301 atcgcggcaa tcgccgcagc tttcgtcgtc tccagatcaa cgacaagcga gccgcgcggt
      361 acatgtccct ttgtctctat gcggccggtt gcggcgaagc ttcctccgcc gatgtcaccg
      421 acgatcagcc gatcgagttg cagtccgttc ggatcgactt gcaggtgcac gcgcgcgtct
      481 cttgcatcga ccccgctgaa cgagatgcgt ccgacctcga tcgcgagcgt catttcgcgc
      541 gggcgttcaa acgaagaccc cgcaagcaag ccacggccaa aggcgaccgc agcgtcgata
      601 tcgagttgcg gtgcctttag ttccgcatcg agttttgccg ggcgatcgcc tgacgccagc
      661 gtgtaggcaa gccgtcccgt tacaggtttg cggtcgaatt cggccttgag cccgtcaacc
      721 gcaattctat cgctggcgag tgtgatatcg ccgtgcaggc ttaatggccg aagatcgttt
      781 tgcccggttt cggtccggcc ctcgagccag agggccagcg ctctgggatc ggaggactcg
      841 atttgtgcgg gtccagtaaa cgcaacgcca tcgctgccga cgtcgaggcg cccgctgagc
      901 cgcacctgcg tcaggccagg cgcccgaaat tcaaggcggt cgagactcca tccgtttgcg
      961 ttgccgctga tgtcaccgcg caggttctgc aagccgttgc cgccgagagt cacctgatcg
     1021 atgccaatac cgagctggat cggcagcggc gcgcgaaatg cgcttgcggc gagcgccgta
     1081 agttggcgga tcacggcagt gggcgtctgc ttctcgctcc cgccgccaga aacagcgcgg
     1141 tccacatcga tctgtcgtcc ggacaggacc gcgttaagcc tcggcctcgc gccaaatttg
     1201 cagtcggcca caccggtcag cctgaccccc tgctcttccg agccatattg gaattcaatg
     1261 tcctgcatca gcgcggattg cgccgttgcc ttgatttttc cgttgacccg ccacggctga
     1321 gtgacatcgc cggctgcagg tccggggcgg cgtaggccga tgccgacagg gcgcctcaga
     1381 ccgaacgttc cgtcaaatcc gggggcgccg cccgtaacgg caagcgcgcc gtcagcctcg
     1441 aaattgagcg gataatcagt aggatccaca ttaagatgga gcctgaccgc gccgccatcg
     1501 ctgatgcggc caaaagccag ccggtaagga taaagcgcgc cagcgatcgt gaccgcaccc
     1561 tcgcccttca tcgggccgac gagggagcga gcctcgccat tgaaccaaac ccgtccgagc
     1621 gtcaggcttg tgttgctggc cgcgtcggcc agcacaaccg tgccgtcttc gatgctcagc
     1681 cggtcgatcg acagctcatc cggattgaag gtcatcgcga ggttcggcgc gcgcaatcgc
     1741 cctgacgaat ccagtcccag gttgacctgc ggaccgacaa gatgaagttc ggtggcacgc
     1801 cactcgccgc gcatgagcga gccgagcgca aattccacgc caagcgagcg cgcgtggacc
     1861 gcgttgccgc catcgccgat ctggatgtcg tgcaaggtga gccgcggcga cggcagcagc
     1921 cgcgcgtcga tgccgcccgt cacgcgcaca ttgacgccga tcagccgact cgcctccgct
     1981 tcgaacaggg tgcgatggtt gccccagtcg agcagcaacg gtcccaccag cgccgcaacg
     2041 agcgcgagga tgatggcaat cgccaatccg agcagtgtgg tttgcaacgc cgactccagc
     2101 gccggatcgc atccggtacc cgttcgcgct caggttcagt gaaatatatg acagatttgc
     2161 cgctgttggc ggcaaatctg tgtcgcggaa caagtcgccg cgggccctag ctcaaggcga
     2221 cgatcttgcc cgggttcatg atgttttgcg gatccagcgc acgcttgatc gaggccatga
     2281 ctgcaagcgc ggcggcgcca tgttcggcca ccagatattt cattttcccc tgcccgactc
     2341 catgctcacc ggtgcaggtc ccatccatgg cgagtgcgcg ttcgaccagc cgctccgaga
     2401 gagccttggc gcgcctgatc tcatcggcgt cgttcatgtc gacaagaagc gtcagatgaa
     2461 aattaccatc gccgacgtgg ccgacaattg gtgccatgat ccggctcgct tcgatgtcgc
     2521 gctgcgtttc agtgacgcac
//
LOCUS       DASSRL010000505         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1115630, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000505 DASSRL010000000
VERSION     DASSRL010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115630"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFL49_10285"
     CDS             complement(<1..797)
                     /locus_tag="VFL49_10285"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912409.1"
                     /translation="MPAVAIEFDGVGLTFAPASAAPTTALEAVSCGFEPGKVTALIGP
                     SGCGKSTLLQIARGFLAPTRGTLRFLERPDGKATTRPAMSTVWQAFNLFPWLTVIDNV
                     AFGLDLIAIAKSERDARARRAIAAVDLTGFEDRYPRQLSGGMRQRVGLARALVMEPEV
                     LLLDEPFGALDAQTRLVLQEQLARLVETSGTTALLVTHSIEEAILLADTILVMTARPG
                     RIAARIEVDLPRPRSVATTRDPGFSALFDRIYGLLRDEVMRAMVSGQG"
     gene            complement(813..1868)
                     /locus_tag="VFL49_10290"
     CDS             complement(813..1868)
                     /locus_tag="VFL49_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912410.1"
                     /translation="MPMSIDRRGLLGGSAAIGLTTAFPRRLRAQEPRTKIRVGLVRLI
                     SSGPIFIAEAKDFFGKVNLDVELKYFADGALAMPALVAGELDLTASTLNAGLFNAVAK
                     GAPYKLILDRGIEKPGSGSMTIVASNAMYEAGYNATDKAKLLKDKKIAIQAPGSIDQY
                     LFGRAAEKAGLDPTKDLNWSSGMPYPDMIKLMGAGQADAANIPVPLAYLAEKNNVGKI
                     IGAGSDIEPNAQLACWVMSSKFLADNKSAAVRFAMVHTYAGRLFNKAAAAKDADVIKI
                     ISDATKVPAPLIERAAPRWTWFDEKGMPNVESCVEQGKFWNKIKLVNANVTKEQLFDL
                     SPATEADARLSKSNPFT"
     gene            2025..>2725
                     /locus_tag="VFL49_10295"
     CDS             2025..>2725
                     /locus_tag="VFL49_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET7912411.1"
                     /translation="MPSTILDSAVFRDIFSTEAMRNVFSDENRVQKYLDFEAALARAQ
                     ARLGIIPKEAAAEIVRHCTADKIDMAKLKEATEKIGYPVLPVVQQLVKLCKDGLGEWS
                     HWGATTQDITDTATIMQIREALTIVEKDIDGICDGLASLSKKYRDTPMAGRSNLQQAV
                     PITFGYKMATMLAAFERHKQRLKEMKPRVLVGEFGGAAGTLSSLGKDGLKVQDELMKE
                     LKLGQPEISWHTVRDR"
BASE COUNT          544 a          840 c          830 g          511 t
ORIGIN      
        1 ccctgcccgc tgaccatcgc gcgcatgact tcgtcgcgca gcagcccgta aatgcgatca
       61 aacaacgcgg aaaatccggg gtcgcgtgtc gttgcgacgc tgcgtggccg cggcaggtcg
      121 acctcgatcc gggcagcgat acgcccgggc cgcgcggtca tcaccaggat cgtgtcggcc
      181 aacagaatcg cttcctcgat cgaatgcgtg accaggagcg cggtggtgcc gctggtttcg
      241 accagccgcg ccagttgttc ctgcagaacc agacgtgtct gtgcatcgag cgcgccgaat
      301 ggctcgtcga gcagcaagac ttccggctcc atcaccagcg cgcgggcgag cccgacgcgc
      361 tggcgcatgc cgcccgacag ttggcgcgga taacgatcct caaagccggt gagatcgacg
      421 gcggcaattg cccgccgcgc gcgggcgtcg cgttcgctct ttgcaatagc gatcagatcg
      481 agaccgaagg cgacgttgtc gatcacggtc agccatggaa acaggttaaa ggcctgccac
      541 accgtggaca tggcgggccg tgtcgtcgct ttgccatcgg gtcgctcgag aaagcgcaaa
      601 gtgccgcgcg tcggcgcaag aaagccgcgc gcaatctgca gcagcgtgct cttgccacag
      661 ccgcttggcc cgatcagcgc agtcaccttg ccgggctcga agccgcagct caccgcttcc
      721 agcgcagtcg tgggtgcagc agacgctggc gcgaacgtca gccctacacc gtcgaactcg
      781 atcgcaaccg ccggcatgcc aatccgcggc aatcaggtga acggattgct cttgctcaat
      841 ctcgcgtcgg cttccgttgc cggcgacagg tcaaacagct gctcttttgt cacgttggcg
      901 ttgaccagct tgatcttgtt ccagaacttg ccctgctcga cgcaggattc gacattcggc
      961 attccctttt catcgaacca cgtccaacgc ggggccgccc gctcgattaa tggcgccggc
     1021 accttggtgg cgtccgagat gatcttgatc acatcggcat ccttggccgc tgccgccttg
     1081 ttgaacaaac gtccggcata agtgtgcacc atcgcaaagc gcacggccgc agacttgttg
     1141 tcggcgagga atttcgagga catgacccag caggcaagtt gagcgttagg ctcgatgtcg
     1201 gagccggcgc cgatgatttt tccgacattg tttttttcgg caaggtatgc gagcggcacc
     1261 gggatgttgg cggcatctgc ctgccccgcg cccatcagct tgatcatgtc cggatagggc
     1321 atcccgctgg accagtttag gtccttcgtc ggatcgagac cggctttttc ggcagcgcgt
     1381 ccgaacagat actggtcgat gctgccggga gcctggatgg cgatcttctt gtccttgagc
     1441 aacttcgctt tgtcggttgc gttatacccc gcctcgtaca tggcattgct ggcgacgatc
     1501 gtcatcgacc ccgaaccggg tttttcgatg ccgcgatcaa gaatgagctt gtaaggagcg
     1561 cccttggcaa ccgcattgaa cagacccgcg ttgagcgtgg aggccgtgag atcgagctca
     1621 ccggcgacca gcgcaggcat agccagggcg ccgtccgcaa aatacttcag ctcgacgtcg
     1681 aggttgacct tgccgaagaa gtccttcgct tcagcgatga agatcgggcc ggaggagatg
     1741 agacgcacca gaccgacccg aattttcgtg cgcggctcct gcgcacgcag ccggcgtgga
     1801 aacgcggttg tcaaaccgat agctgcgctg ccgccaagca gccctcgacg atcaatggac
     1861 atcggcatgg ttggtcctcc cgacatgttt gcttgaagtt gttttgatca gcgtagcaac
     1921 gccattgcgc agccgcaacg gcgcgccaat gcggcacttg gacgtcacat gagcttgatc
     1981 gggcgcgccg aacggctaac gtgcaaccga caggagaaaa tcggatgccc tcaacgatcc
     2041 tcgattccgc cgtgttccgc gacatcttct cgacagaagc gatgcggaac gtcttctcgg
     2101 atgaaaaccg tgtgcagaag tatctcgatt ttgaagccgc gcttgcgcgc gcgcaggctc
     2161 ggctcggaat cattccgaag gaagcggccg ctgaaatcgt gcgtcactgc accgccgaca
     2221 agattgacat ggcgaaactc aaggaggcga ccgagaagat cggctatccg gttctgccag
     2281 tcgtccagca gctggtgaag ctctgcaaag acgggctcgg cgaatggtcg cattggggcg
     2341 ccaccacgca ggacatcacc gataccgcca cgatcatgca aattcgcgaa gcgctgacga
     2401 tcgtcgagaa ggatatcgac ggaatttgcg atggccttgc ctcgctctcg aaaaagtacc
     2461 gcgacacgcc gatggccgga cgcagcaacc tgcagcaggc ggtgccgatc accttcggat
     2521 acaagatggc gacgatgctc gccgcattcg agcggcacaa gcagcgcctg aaggaaatga
     2581 agccacgcgt gcttgtcggc gagttcggcg gtgccgccgg cacgctatcc tccctcggca
     2641 aggacggcct gaaagtgcag gacgagctca tgaaggagct caagctcggc cagccggaaa
     2701 tttcatggca caccgtgcgt gatcg
//
LOCUS       DASSRL010000506         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_713175, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000506 DASSRL010000000
VERSION     DASSRL010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713175"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFL49_10300"
     CDS             complement(<1..422)
                     /locus_tag="VFL49_10300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912412.1"
                     /translation="MLQSTMPSIESKKSWAVATAALVTMLMAFGAAWITAVALKDIAA
                     EVDGVRSIPALASALAWLGSGFGGILMGRLADKVGTRWTVIFGALMVGLGLTISTLGP
                     PWPLWIGHGLFVGLIGLGGINAPMYIYVSRWFDRRRGSA"
     gene            694..>3775
                     /locus_tag="VFL49_10305"
     CDS             694..>3775
                     /locus_tag="VFL49_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HET7912413.1"
                     /translation="MPLRPVSLDDKYDLNQSHIFVTGYQALIRACLMQKERDRRAGLN
                     TAGYVTGYRGSPLGGLDQQFMRASRQLAAADIKFQSGLNEELAATALWGTQQAELRGE
                     GKFDGVFGMWYGKGPGVDRTGDAFRHANLAGSSRYGGVIALMGDDHTAESSTTAHQSE
                     FHFVDVMMPILNPAGVQEFADYALYGWEMSRFTGAWSALKCMHETVESTGVVDASLDR
                     IRILMPDDFPMPEGGLNIRLADSFLGQEARLHDFKRDAMLAFVRANKLNKIITTGGRA
                     AKIGIITTGKAFLDVRQALDELGIDELKCNDLGLRIFKVACVWPIGRQELAEFAQGLD
                     LIIVVEEKRSLIEVQVREELYGSANQPVCIGKKDEQGNWLFPVKGALDPNDVAICIGE
                     RLLRYGSNDEIASHVTRLKSAQKALAETTDVAQRIPYFCSGCPHNTSTRVPEGSRAYA
                     GIGCHYMAQWMDRKTLGYTQMGAEGANWIGEAPFSTRDHIFQNLGDGTYNHSGYLAIR
                     AAIASGVTMTYKILFNDAVAMTGGQANDGGLTVPQIAAQVAAEGAKRIAVVTDEPWKY
                     AKDIEWPRGLTVHHRDELDAVQRELATISGVSVLIYDQTCAAEKRRRRKRGAFPDPDK
                     RVVINDLVCEGCGDCGIKSNCVSVQPLETEWGRKRTIDQSSCNKDYSCIKGFCPSFVT
                     VHGAKLKKGESIAQPGDWPDLPAPSAPLINHPYGIIVTGIGGTGIVTIGAIVGMAAHL
                     EGKGVGVIDMAGLAQKGGAVYSHIRIANTPEEIHAIRIAAGGADLVIGGDIVVAGAKK
                     VLGAMKAGITQVVVNTAEFLPGDFTRNTEFSLPTERLKRAIGGHAGRDRSHFVDASRL
                     ATALLGNSIGANMFMLGYAYQLGALPLSADAIERAIEMNGEAVPMNIAAFRYGRRAAI
                     DPKALEALIEPRPQEQNDSLKLSQSFAETVDRRVEFLTAYQNAGYARRYRNWVEKVRT
                     VEAEKAPGQCGLAEAVARYLFKLMAYKDEYEVARLYADDAFLKQVNNELGGENLR"
BASE COUNT          783 a         1157 c         1147 g          688 t
ORIGIN      
        1 gccgagccgc gccggcgatc gaaccagcgg ctgacgtaga tgtacatcgg tgcattgatg
       61 ccaccgagtc cgatcagtcc gacgaacaac ccgtgcccga tccacaaggg ccatggcggc
      121 ccgagtgtcg agatggtcag gccgagcccg accatcagcg cgccgaagat gaccgtccat
      181 cgcgtaccga ccttgtcggc gagacgtccc atcaaaatgc cgccgaaacc tgaaccgagc
      241 caggcaagcg cgctggccaa cgcagggatg gaccggacgc cgtcgacctc cgccgcgatg
      301 tccttaagcg caaccgccgt gatccacgcg gcaccgaagg ccatgagcat ggtgacgagc
      361 gccgccgtgg caacggccca cgacttcttt gattcgatgg aaggcattgt ggattgcaac
      421 atcaggcgga acccggctgg gcgatgttgt ggcaggccgg ccctgctttg accacccaca
      481 gcggcggggg tctgccttgt ctgctgcatt gcgccaaaat gcgcgcggag cgcgttcctc
      541 gaaatttcct tttagcggcc gctgaaattt cgaaattgac gcggcgaccg cgacgtatag
      601 ccttcaccgc gatttctgcg ctttgcgccg ccgaggccaa gcttatatga gtggggcgcc
      661 ggcaccgcat ccgccggatt tacaaggcgt ccaatgccgc ttcgaccggt cagtctcgac
      721 gacaagtacg acctaaacca gtcgcatata tttgtgaccg gttatcaggc gttgatccgt
      781 gcttgcctga tgcaaaaaga gcgagaccgc cgcgccggac taaatacggc tgggtatgtt
      841 acgggctacc gcggctcacc ccttggaggg ctcgatcagc aattcatgcg cgcgtcccgc
      901 cagctcgcag ccgcggatat caaattccag tccggtctca atgaggaact tgccgccacc
      961 gccttatggg gcacccaaca ggccgaattg cgaggcgaag gcaaattcga tggcgtattc
     1021 ggcatgtggt acgggaaggg ccccggcgtc gaccggacgg gcgatgcgtt ccgtcatgcc
     1081 aacctcgcgg gttcatcaag atacggcggc gttatcgcct tgatgggcga cgaccacacc
     1141 gcggagtcct cgacgaccgc gcatcagtcg gaattccatt tcgtcgacgt gatgatgcct
     1201 attctcaacc ccgcgggcgt gcaggagttt gccgattacg cgctctatgg ctgggagatg
     1261 tcgcgtttca ccggcgcctg gagcgccctc aaatgcatgc atgagacggt cgaatcgacg
     1321 ggagtggttg acgccagcct cgaccgcatc agaatcctga tgccggacga tttccccatg
     1381 ccggagggcg gactgaatat ccgcttggcc gattcttttc ttggccagga ggcgcggctg
     1441 cacgatttca agcgtgacgc gatgctcgcc ttcgtgcgtg ccaataagct caacaagatc
     1501 atcacgacag gcggccgggc agcgaaaatc ggcattatca caaccggcaa ggcctttctc
     1561 gacgttcgcc aggcgcttga tgagctcggc atcgacgaat tgaaatgcaa cgatcttggc
     1621 ctgcgcatct tcaaagttgc ctgcgtgtgg ccgatcggac gccaggagct tgcagagttt
     1681 gcgcagggac tcgacctcat catcgtggtc gaggaaaagc gctcgctgat cgaagttcag
     1741 gtccgcgaag agctctatgg ctcggcaaac cagcccgttt gcatcggcaa gaaggacgag
     1801 cagggcaatt ggcttttccc cgtgaagggt gcgctcgatc cgaacgacgt cgccatctgt
     1861 atcggcgaaa gattgcttcg ctacggatcg aacgacgaga ttgcttcaca tgtgacgcgc
     1921 ctgaaatcgg cgcaaaaggc cctcgccgaa acgaccgatg tcgcccagcg tattccctat
     1981 ttctgctcgg gctgcccaca caacacatcg acgcgcgtgc ccgagggcag ccgagcctat
     2041 gccggcatcg gctgccatta catggcgcag tggatggatc gtaagacgct cggctacacc
     2101 cagatgggtg ccgaaggcgc caattggatt ggcgaagcgc cattttccac gcgcgatcac
     2161 attttccaga atctcggtga cggcacctat aaccattcgg gctaccttgc tatccgcgct
     2221 gcgatcgcct ccggcgttac gatgacctac aagatcctgt tcaacgacgc ggtggcgatg
     2281 accggtggac aggccaacga tggcggcctg acggtgccgc agattgccgc gcaggtggct
     2341 gccgaaggcg cgaagcggat agccgtagtc accgatgagc cctggaaata tgcaaaagac
     2401 atagagtggc cgcgtgggct gactgtgcat cacagggacg aactggacgc agttcagcgc
     2461 gaactggcca ccatttccgg cgtgagcgtg ctcatctacg accagacctg cgcggctgaa
     2521 aaacgccgcc ggcgcaagcg cggcgccttt cctgatccag acaagcgcgt cgtcatcaat
     2581 gatctcgttt gcgaaggttg cggcgactgc ggcatcaagt cgaactgcgt atcggtgcag
     2641 ccactcgaaa ccgaatgggg ccgcaagcgc acgattgacc agtcaagctg caacaaggac
     2701 tactcctgca tcaaaggctt ttgcccttcg ttcgttaccg ttcatggagc gaagctgaaa
     2761 aagggcgaaa gcatcgcgca gccaggcgac tggcctgacc tgccggcacc gtctgccccg
     2821 ctcatcaatc atccctacgg aatcatcgtg accggcatcg gcggcaccgg catcgtcacc
     2881 atcggcgcca ttgtcggcat ggcggcgcac ctcgaaggga agggcgtagg cgtgatcgat
     2941 atggccggcc tcgcgcagaa aggtggcgcc gtctacagcc acattcgcat cgccaacacg
     3001 cccgaagaaa tccacgccat tcgcatcgct gccggcggcg cggacctcgt gatcggcggc
     3061 gacatcgtcg ttgcgggcgc caagaaagtt cttggtgcaa tgaaagcggg aatcacgcag
     3121 gtcgtcgtga atacggcgga atttctgccg ggcgatttca cgcgcaacac cgaattttcg
     3181 ctgccgaccg agagactcaa gcgtgccatt ggcggtcacg ccggccgtga tcgcagccat
     3241 ttcgttgacg cgagccggct tgcgacggca ctgctcggaa attcgatcgg cgccaacatg
     3301 ttcatgctcg gctatgccta tcagctcggg gcgctgccgt tgtcggcgga tgcgatcgag
     3361 cgcgctatcg aaatgaacgg cgaagccgtg ccgatgaaca ttgccgcgtt ccgctatggg
     3421 cgccgcgccg caatcgaccc caaggcactt gaggccctga tcgagccgcg gccgcaggag
     3481 cagaacgatt cgctcaagct gtcgcagtca tttgccgaaa ccgtcgaccg ccgagtggag
     3541 tttctgacgg cctatcagaa cgccggctac gcgcgacgtt accgcaattg ggtcgagaaa
     3601 gtgcgcactg tggaagctga aaaggctccc ggacaatgtg ggctggcgga agcggtggcc
     3661 cgttatctgt tcaagctgat ggcctacaag gacgaatacg aggtcgcccg cctctacgcc
     3721 gacgacgcct tcctcaagca ggtgaacaac gagctcggcg gcgagaacct gcgcc
//
LOCUS       DASSRL010000507         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_818402, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000507 DASSRL010000000
VERSION     DASSRL010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818402"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFL49_10310"
     CDS             complement(<1..932)
                     /locus_tag="VFL49_10310"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET7912414.1"
                     /translation="MSPPVPPNDRERNRFAARARRYAKVGTQLSGTAARFAGARFFGV
                     PLDRAANALELAAALGGLKGPIMKVAQLLATIPDALPPEYANELIKLQSQAPPMGWAF
                     VKRRMGAELGADWQKKFSEFEHHPSAAASLGQVHRARALDGSDLACKLQYPEMQSAVE
                     ADLSQLQLLFAIRKRFDPVIDTTEIAVEIGARLREELDYFREARHVALYGNMLKDEDA
                     IRVPKVWPTLSTGRLLTLDWMTGRKLLEHKSDPLETRNRLATAMFTAWWFPFSRFGVI
                     HGDPHLGNYTVFDDAKGKPAGINLLDYGCIRIFPP"
     gene            complement(978..>2151)
                     /locus_tag="VFL49_10315"
     CDS             complement(978..>2151)
                     /locus_tag="VFL49_10315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET7912415.1"
                     /translation="DGKKRRAAAEALAKTFKENLRPFTLITNTLAKDKEISDRWRGFK
                     DIADARHLSNRVEPEVVEALVSAVRAAYPKLSHRYYALKAKWFGRKRLPHWDRNAPLP
                     KVGQRTIPWGDARATVLTAYGAFSPKMADVADQFFTKSWIDAPVRPGKQPGAFAHPTV
                     PSAHPYVLLNYMGKPRDVMTLAHELGHGVHQVLAAPNGPLMAPTPLTLAETASVFGEM
                     LTFRKLLADTTDKKQRKAMLAAKVEDMINTVVRQIAFYSFERKVHGERRGGELTSDKL
                     CDLWMSVQSESLGPAIELKPGYETFWAYIPHFVHSPFYVYAYAFGDCLVNSLYAVYEK
                     SASGFAERYLEMLAAGGTKHHAELLAPFGLNARDPKFWDGGLSVIARLIDELEALE"
BASE COUNT          401 a          681 c          655 g          414 t
ORIGIN      
        1 ggcgggaaaa tccggataca tccgtagtcg agcagattga tcccggcggg cttgcccttg
       61 gcatcatcga acaccgtgta gttgccgaga tgcggatcgc cgtgaatgac gccgaaacgg
      121 ctgaagggaa accaccaggc cgtgaacatc gccgttgcaa gccggttgcg cgtctcgagg
      181 gggtcgctct tgtgctccag cagtttccga cctgtcatcc agtcaagcgt cagcagcctg
      241 ccggtcgata gcgtgggcca gacctttggt acacggatcg catcctcgtc tttcagcata
      301 tttccataaa gcgcgacgtg ccttgcttcg cggaaatagt cgagctcttc ccgcaggcgc
      361 gcgccgattt cgaccgctat ttcagtcgta tcgatgaccg gatcaaaacg cttgcggatc
      421 gcgaacagca attgcagctg gctcaggtcc gcctcgaccg ccgactgcat ctcggggtac
      481 tgcagcttgc aggcaaggtc gcttccatcc agcgcacgcg cacgatgcac ctgcccgagt
      541 gaagcggccg cggacggatg gtgctcgaac tccgaaaatt tcttctgcca gtcggcgccg
      601 agctcggcac ccatgcgccg tttgacgaag gcccatccca tgggcggcgc ctgactctgc
      661 agcttgatca gctcgttggc gtattccggc ggcagcgcat caggaatggt ggcaagcagc
      721 tgcgccacct tcatgatcgg acccttcaac ccgccgagcg cagcagcgag ctcaagcgcg
      781 ttggcggcac gatccagcgg cacgccaaag aagcgcgcac cggcaaatct tgcggctgtg
      841 ccactgagct gcgtgccgac cttggcgtag cgccgggcac gggcagcaaa gcggttccgt
      901 tcacgatcgt tcggcggcac cggcgggctc atggcccacc atctaatcga ttttggcctt
      961 gaaaataagg gctggactta ctcgagagcc tcaagttcgt cgatcagcct ggcgatgacg
     1021 ctcagcccgc cgtcccagaa cttcggatcg cgcgcgttga ggccgaacgg cgccagcaat
     1081 tccgcatgat gcttggtgcc tccagccgcg agcatttcaa ggtaccgctc ggcgaagccg
     1141 ctcgcggact tttcgtagac ggcatagagc gagtttacca ggcagtcgcc gaatgcgtag
     1201 gcgtagacgt agaagggcga atgcacgaaa tgcgggatat aggcccagaa ggtttcgtaa
     1261 cccggtttga gctcgatcgc cggacccagg ctttcgcttt gcaccgacat ccagagatcg
     1321 caaagcttgt cggaggtcag ttcgccacca cgccgctcgc cgtgcacctt gcgctcaaaa
     1381 ctgtagaagg cgatctggcg cacgaccgta ttgatcatgt cctcgacttt ggcggcaagc
     1441 atggccttgc gctgcttctt gtcggtggta tccgcgagca gcttcctgaa cgtgagcatt
     1501 tcgccgaaca ccgaggctgt ttcggcgagc gtgagcggcg tcggcgccat cagcgggccg
     1561 ttcggcgcgg caagcacctg atgcacgccg tggccaagct cgtgcgctag cgtcatcacg
     1621 tcgcgcggct tgcccatata gttgagcagg acgtagggat gcgccgacgg caccgtcgga
     1681 tgcgcgaacg cgcccggctg cttgcccggg cgcacgggcg catcaatcca gctcttcgtg
     1741 aaaaactgat cggcgacgtc agccatcttg ggtgaaaacg cgccataggc tgtcagaacg
     1801 gtggcacgcg catcgcccca cgggatcgtc cgctgtccga ctttcggcag cggcgcattg
     1861 cgatcccagt gcggcagccg cttcctgccg aaccactttg ccttcagcgc gtaatagcgg
     1921 tgcgacagct tcgggtaagc cgcacgcacc gcggaaacca aagcctccac gacctcgggc
     1981 tcgacccggt tcgacaagtg ccgcgcatcg gcgatgtcct tgaagccgcg ccagcggtcc
     2041 gaaatttcct tgtccttcgc gagcgtattc gtgatgagcg tgaacggccg cagattttcc
     2101 ttgaacgttt tcgccagcgc ctcggcggct gccctgcgtt tcttgccatc g
//
LOCUS       DASSRL010000508         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_608218, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000508 DASSRL010000000
VERSION     DASSRL010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608218"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFL49_10320"
     CDS             complement(<1..780)
                     /locus_tag="VFL49_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7912416.1"
                     /translation="MTAMLDIDQLRTFIAISETGSFTKAAEVVNKTQSAVSMQMKRLE
                     ERLERPIFARDGRASKLTEDGQRLLDYARRIVKLNVETLAAFSDAELSGRVRLGVPDD
                     YADRYLPEIMARFSRGYPAVELSVICEPSVDLLERIDANEIDLAIVTNCESKRASETF
                     RRERLLWVTSNRHSTHLEERMPLALGRPSCTWRRIAIERLEAIGRPFRVLYSSSNAGA
                     VAAAVLAGLAVSVLPESGLRPGMRVLSASDGFPDLPSCRIGL"
     gene            991..1350
                     /locus_tag="VFL49_10325"
     CDS             991..1350
                     /locus_tag="VFL49_10325"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HET7912417.1"
                     /translation="MSAQYPVLPVAGGALLRAFASLVALVTHWLKELARARRHRREAH
                     ALAGLDRRMLADIGITGSDVRDAFSEPFWDDPTALLRERALERRLSASVRRITLDAGA
                     NPAFRRPATDRPSRETL"
     gene            1633..2133
                     /locus_tag="VFL49_10330"
     CDS             1633..2133
                     /locus_tag="VFL49_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HET7912418.1"
                     /translation="MLALAGHVQAQSGFDRIGGDYLSFQVRSGDPAVCASRCERDGRC
                     RAWSFSYPRTANTAATCWLKNYVPPRNEDKCCVSGVRGAGVTEPRTGPVEYSIDRLGG
                     DYRNLDVAADPAGAPCRAACEADNKCRAWTYVRQGYISSSPRCYLKDRITRPRHKPCC
                     ISGVVR"
     gene            complement(2130..2888)
                     /gene="lepB"
                     /locus_tag="VFL49_10335"
     CDS             complement(2130..2888)
                     /gene="lepB"
                     /locus_tag="VFL49_10335"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7912419.1"
                     /translation="MPKKQSGLSGWIRNIADIAVIVLIVLAAKTAIAEPFYVPSGSME
                     PTLLIGDELLAMKYPYGYSSASLPSFFVFPNTPRVFAALPKRGDVVVFRWPGDRSQVW
                     VKRVIGLPGDRIAVSRGQVRIDGEPVGIKPAGAGAMETEDGSTIAVARFIETLPGGHE
                     HPIFKMSAHGEYDDMPEVVVPPGHVFVMGDNRDNSADSRVPVRAGGVGLLPVEDLVGR
                     VDAIVGSWDLAVKNRPIQDWPSGLRLSRFFTSVR"
     gene            complement(2905..>3278)
                     /locus_tag="VFL49_10340"
     CDS             complement(2905..>3278)
                     /locus_tag="VFL49_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7912420.1"
                     /translation="PMPRMTNTYMLAGKHDPTEIIASVKNGLFAVSFGGGQVDITSGK
                     YVFQCTEAYKIENGKIGAPVKGAMLIGNGPTDLHRVTMIGNDFALDPGIGTCGKNGQG
                     VPVGVGQPTLRMDKITVGGTA"
BASE COUNT          612 a         1048 c         1012 g          606 t
ORIGIN      
        1 cagcccgatg cggcaactcg gcagatcggg gaaaccgtcc gacgcggaca gaacgcgcat
       61 gcccggacgc aggccagact ccggcagcac cgaaaccgca agcccggcga gcaccgcggc
      121 ggcaacggca ccggcattcg aactcgagta gagcacgcgg aacggacggc cgatcgcttc
      181 cagccgttcg atcgcaatgc gccgccaggt gcagctcggc cgtccaagcg caagcggcat
      241 gcgttcctcg agatgagtcg aatggcggtt cgacgtcacc cagagcagcc gctcgcggcg
      301 gaatgtttcc gaagcgcgct tgctttcgca attggtgacg atggcaaggt cgatttcgtt
      361 ggcgtcgatg cgctcgagca gatcgaccga tggctcgcag atcaccgaca gctcgaccgc
      421 cggatagccg cgcgagaagc gcgccatgat ctcgggcaga tagcggtcgg cataatcgtc
      481 cggcacgccg agccgcacac gtcccgacag ttcggcgtcg gaaaacgcgg ccagcgtctc
      541 gacattgagc ttcacgatgc gccgcgcgta gtcgagcagg cgctggccgt cctcggtcag
      601 cttcgaggcg cgtccgtccc gcgcgaagat cgggcgttcg aggcgctcct cgagccgctt
      661 catctgcatc gagacagcgg actgggtctt gttcacaact tcggccgcct tggtgaagct
      721 gccggtctcg gagatggcga tgaacgtgcg cagctggtcg atatcgagca ttgcggtcat
      781 ggcggcggat cctgagtggc ccaattgccg ggcggggtga ccatatcatc gtttctgatt
      841 gatcagatta aaaacattcg tttcactaat caatccccgc agtgcaatat tcgggcttcc
      901 agaggaatcg ggtggggatt tcgggtggtt ctgggcagcg ccaatagcga ttttggtgcc
      961 gtaaccactg gactgaattg gagatttgct atgagtgccc aataccccgt tttaccagtc
     1021 gccggcggtg cgctgttgcg ggcgttcgcc tccctcgtgg cactggtcac ccactggctc
     1081 aaggaattgg cgcgggccag gcggcaccgc cgcgaggcac atgctctcgc cgggctcgac
     1141 cgccgcatgc tcgcggatat cggcatcacg ggctcggatg ttcgtgatgc cttctccgag
     1201 cccttctggg acgacccgac cgcgctcttg cgcgagcgcg cattggaaag acggctgagt
     1261 gcctcggtcc ggcgcatcac gcttgacgcc ggcgccaacc ccgccttccg gcggcctgcg
     1321 accgaccgcc cgtcgcgcga gacgttatga ggtcaagcaa acggtcgagc cctctcctcg
     1381 gccgctagat ccgacacccg cgatcatccc ctctcgcggc cggatcccgc gcccgccggc
     1441 ccctccggcg ggcgctttat ttaaatttaa ttcgccgcgc gaaaattgct cgctgacatt
     1501 gttgtgtctg cacaacggcg gatgactgag ttaccatgaa ttcggggcaa ggcttcatac
     1561 cgagtctttt caggattgat cgcacgcgca atgcgaattc cgatcaagct aggggccgcg
     1621 ctcacattcg cgatgcttgc ccttgccggt catgtgcaag cgcaaagcgg atttgaccgc
     1681 atcggcggcg actacctgag ctttcaggtg cgcagcggcg atcccgccgt ctgtgcgagc
     1741 cgctgcgagc gcgatggacg ctgccgtgcg tggagcttct cgtatccgcg caccgcgaat
     1801 acggccgcga cctgttggct gaaaaattac gtcccgccgc gcaacgagga caaatgttgc
     1861 gtgtcaggcg tccgcggggc gggcgtgacc gaaccgcgca cgggtccggt ggaatattcc
     1921 attgaccggc tcggcggtga ctaccgcaac ctggatgttg cagccgatcc ggccggtgcg
     1981 ccgtgcaggg cggcgtgtga agccgacaac aaatgccgcg cctggaccta cgtgcggcag
     2041 ggctacatca gttccagccc acgctgttat ctcaaggaca ggatcacgcg gccgcggcac
     2101 aagccatgct gcatttcggg cgtagtgcgc taacgcactg acgtgaaaaa gcgcgacagc
     2161 cgaaggcccg acggccagtc ctggatcggc cgatttttga cggcgagatc ccacgagccg
     2221 acgatcgcat cgacacggcc gaccagatcc tccaccggca gcagtccgac accgccggcg
     2281 cggacaggca cgcggctgtc ggcagaattg tcgcgattgt cgcccatgac gaaaacgtgg
     2341 cccggcggca caacaacctc cggcatatcg tcgtactcac cgtgggcgct catcttgaag
     2401 atcggatgct cgtgcccgcc aggcagcgtt tcgataaagc gcgcgacggc aatcgtgctg
     2461 ccgtcctcgg tttccatcgc gccggctcct gccggtttaa taccgacggg ctcgccgtcg
     2521 atgcgcacct ggccgcggct gacagcgatg cggtcgccgg gcaaaccgat cacgcgcttc
     2581 acccagacct gcgaacggtc gcccggccag cggaagacga cgacatctcc gcgcttggga
     2641 agtgccgcaa agacacgcgg cgtgttcgga aaaacaaaga atgatggaag agacgcgctg
     2701 ctgtagccgt atggatattt catcgccagc aactcgtcgc cgatgagcaa tgtcggctcc
     2761 atcgagcccg atggaacgta gaatggctcc gcaatggcgg tcttggccgc aagcacgatc
     2821 agcacgataa ccgcgatatc ggcaatattt ctgatccagc cgctcaagcc cgactgtttc
     2881 ttcggcatat ccgaactccc gcgatcaggc cgttccgccg acggttattt tgtccatgcg
     2941 cagcgtcggc tggccgacgc cgaccggcac cccctggccg ttcttgccgc aggtgccgat
     3001 gcccggatcg agggcaaaat cattgccgat cattgtgacg cgatgaagat cagtcggccc
     3061 gttgccgatc agcatcgccc cttttaccgg agcgccgatc ttgccgtttt cgatcttgta
     3121 ggcttcggtg cattggaaca catatttccc cgaggtgatg tcgacctgtc cgccgccaaa
     3181 gctgacagca aaaagcccgt tcttcaccga ggcgatgatc tccgtcggat catgtttccc
     3241 tgcgagcatg taggtgttgg tcatccgtgg catcggca
//
LOCUS       DASSRL010000509         8215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_468796, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000509 DASSRL010000000
VERSION     DASSRL010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468796"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFL49_10345"
     CDS             complement(<1..807)
                     /locus_tag="VFL49_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912421.1"
                     /translation="MASPIRTVVELVQRERTVATTKSFSQLMQLVQTGSRSLFGSASA
                     YRAMSLTALAWAAVLEAIRFTNKINPDIKQHDATNHHDLGRAAPPRSEQAIQENDQSI
                     AGAHADRHAKFEDADESHHDSRVNISSPPIGSSTDPQILAELRHFSPAVVLTPVQASP
                     APGPAVQTQIVEAATALPAPVISIPDATATAPAPVTSPVVSSVIASGSGIDGSGNGDL
                     NAGHVVTLTVTMSEVVTVAGGVPTLSLSSGGTATYTGGSGTSALTFSYTVG"
     gene            complement(952..1671)
                     /locus_tag="VFL49_10350"
     CDS             complement(952..1671)
                     /locus_tag="VFL49_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7912422.1"
                     /translation="MTVSVNDIEISVESGASEELTAARELLRQRAIAVGLLGAAVKEG
                     TAVDKAIEALLAREVVTPSPTEEECRRYYESHPREFQSGDLVHARHILFQVTPSVRVP
                     EMRARAEQTLNDLLREPARFDALARELSNCPSGQHGGNLGQIGRGDTVPEFEKALFRF
                     GASGVLRDLVRTRYGFHIVAVDRSIPGKALPFEAVHGQIAERLRASVEERAMRQYISV
                     LAGQAKITGVDLNAAGTPLVQ"
     gene            complement(1668..2387)
                     /gene="narI"
                     /locus_tag="VFL49_10355"
     CDS             complement(1668..2387)
                     /gene="narI"
                     /locus_tag="VFL49_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HET7912423.1"
                     /translation="MLSNSYVNTFLFGIYPYLCLSILLIGSLIRFDREPYTWKSDSSQ
                     MLRKRQLRLGSNLFHYGVIIVILGHFAGFLAPHWTIDWALSPVAHQLLAMVVGGIAGL
                     VAIVGLTILLVRRLGDPRIRLNSRKWDIAVALMLWLQLMLGLLTVPLSAFHMDGALFE
                     MLTSYVKGIVTLDGSASALVIDVPLTYKLHILLGFTLFLVSPFTRMIHIWSGAGALAY
                     LFRPYQIVRTPRTRAKEPVRS"
     gene            complement(2404..3039)
                     /gene="narJ"
                     /locus_tag="VFL49_10360"
     CDS             complement(2404..3039)
                     /gene="narJ"
                     /locus_tag="VFL49_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HET7912424.1"
                     /translation="MILFRALSALLSYPSEEIRQALPAIADVIRSAPLVAPDQRGGLL
                     DLIDELGGLDLLTAEERYVDLFDRGRALSLHLFEHLHGESRDRGEAMVELRQLYEKAG
                     FELSARELPDYLPVVLEYLSCRDLAEAREMLADCAHILTTIGRSLVARRSRYAAVLQA
                     LIVIAGESPIDVAKVPPVKERTEALDRDWAEQPAFAGAPGSAGQGDGTPAA"
     gene            complement(3049..4581)
                     /gene="narH"
                     /locus_tag="VFL49_10365"
     CDS             complement(3049..4581)
                     /gene="narH"
                     /locus_tag="VFL49_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HET7912425.1"
                     /translation="MKVRAQIAMVLNLDKCIGCHTCSVTCKNVWTSRQGMEYAWFNNV
                     ETKPGIGYPKDWENQERWRGGWERRKSGKIVPKQGGRLAILAKIFANPHLPEIDDYYE
                     PFTFDYEHLHIAPELPTSPVARPLSLVTGRPMEKIEWGPNWEEILGGEFDKRSQDYNF
                     EAVQKDIYGQFENTFMMYLPRLCEHCLNPSCVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWTSGKAEKCIFCYPRIEDGQPTVCSETCVGRIRYLGVLLYDA
                     DGIESAAAVADEQDLYEAQLKLFLDPNDPAVVEQASADGVPDAWLAAAKRSPAYKMAV
                     DWKIAFPLHPEYRTLPMVWYVPPLSPIQSHANAGAIESVGDIPDVRSLRIPVRYLANL
                     LTAGAEAPIVTALERMLAMRIYFRQRQLGEGDGRSVLAQAGLTVAQVEDMHRYLAIAN
                     YEDRFVIPTSHREEAEDAYGLKGSCGFTFGNGCDFGPPHSSLFGGKMGRRKLSPMRPL
                     DT"
     gene            complement(4578..>8215)
                     /locus_tag="VFL49_10370"
     CDS             complement(4578..>8215)
                     /locus_tag="VFL49_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HET7912426.1"
                     /translation="HDKIVRSTHGVNCTGSCSWKIYVKSGVVTWETQQTDYPRTRPGM
                     PNHEPRGCSRGASYSWYLYSASRIKYPMIRGRLIKAWREARKTLKPVEAWAAIVGDTK
                     TATSYKSMRGRGGFVRSTWNEVSEIIAAANVHTIKTWGPDRVVGFSPIPAMSMVSYAS
                     GARYLSLIGGSLLSFYDWYCDLPPSSPQTWGEQTDVPESADWYNSGYIIAWGSNVPQT
                     RTPDAHFFVESRYNGTKVVAVTPDYSEVAKLSDLWLHPKQGTDAALAMSMGHVILREF
                     FLDRQVPYFQDYTRRYTDMPMLVRLRQERDRWVPDRYVRQSDLVGAPKSDRAAWKTVA
                     TDEDSGNLVVPQGSIGYRWPADGEAKGRWNLEPKDGETGEDVRLALSLIDRRDDVVPV
                     AFPYFGGKPHEHFPENDQGGDVLLRNVPVRRIALSDGEAHVATVFDLLCANYGLDRGL
                     GGACAASFDDDVPYTPAWQEPITGVPADKAIAVARGFAENAEKTKGRSMVIIGAGMNH
                     WYHQDMGYRSIINFLMMCGTIGVSGGGWAHYVGQEKLRPQTGWTMLAFALDWVRPPRQ
                     MNGTSFFYAHSDQWRYEKLEVSELLSPLADPAPYRASMIDLNARAERMGWLPSAPQLA
                     VNPLTLAADADRAGVAVKDHVVAGLKTGRIRMSCEDPDDPVNFPRNLFIWRSNLFGSS
                     GKGHEYFLRHFLGTRHGLQGKDLGEMDGVKPQEVAWRDPAPEGKLDLVVTLDFRMSTS
                     CLYSDIVLPTATWYEKNDLNTSDMHPFIHPLSTAVDPVWESRSDWETYKTIARKFSEL
                     AAGHLGIERDVVLTPIQHDTAAELAQPLDVKDWKKGECELVPGVTAPQIAVVERDYPN
                     TYRRFTALGPLADKLGNGGKGIAWNTLDEVKGLGDLNYRVNDGGATDGRPRIETDIDA
                     AEVILYLAPETNGEVAVKAWEALGKITGRDHKHLADMREDEKIRFRDLQAQPRKIISS
                     PTWSGIESEHVSYTAGYTNVNELIPWRTLSGRQQFYQDHRWFRDFGEGLAVYRPPIDT
                     RTVAEMLGKRSNGEAEIVLNFITPHQKWGIHSTYTDNLLMLTLSRGGPIVWISETDAK
                     KAGIVDNDWIELFNLNGAIAARAVVSQRVHEGMCLMYHAQEKIVNVPGSQITGQRGGI
                     HNSVTRTMLKPTHMVGGYAQLSYGFNYYGTVGSNRDEFVIVRKLNKVDWLDHSNPVDR
                     AVEETAS"
BASE COUNT         1599 a         2493 c         2529 g         1594 t
ORIGIN      
        1 gccgaccgtg tagctgaagg tcagcgcact ggtaccggag ccgccggtgt aggtcgcggt
       61 ccctccactg ctgagcgaca gcgtcggcac cccgccggca accgtcacca cttcgctcat
      121 agtgacggtg agcgtgacca cgtgcccggc attgaggtcg ccattgccgc tgccatcgat
      181 gccgctgccc gacgcaataa cggacgaaac aactggcgag gtgacaggtg ccggtgcggt
      241 cgcggtggca tcgggtatgc tgatcaccgg ggccggtaac gcagtggccg cttcaacaat
      301 ttgggtttgg accgcaggtc ccggggccgg ggaagcctga acaggcgtta ggacaactgc
      361 agggctgaaa tggcggagtt ccgcaagaat ttgcggatcg gtcgacgacc cgatcggcgg
      421 gctactgata ttgacgcggc tatcgtggtg cgattcgtcc gcatcctcga attttgcgtg
      481 acggtcggca tgcgcgcctg caattgattg gtcattttcc tggatagctt gctcagagcg
      541 cggcggcgcg gcccgcccga gatcatgatg gttcgtggca tcgtgttgtt tgatgtccgg
      601 attgatcttg tttgtaaacc tgatcgcttc gagcaccgcg gcccacgcca gcgcggtcag
      661 gctcatcgcg cggtaagccg acgcagatcc gaacagcgag cggctgcctg tttgcacaag
      721 ctgcatcaac tgcgaaaagg atttcgtcgt agccacggta cgctctcttt ggactaattc
      781 gacgaccgtt ctgatcggcg atgccaagga cgttcccaat ctgatttctg gattggcacg
      841 ttaaaatcat atgccgccag cgacgaaaaa atcagtaaag ttagatcgaa aagaaaagca
      901 ccctcgttta ggttttattt accatatgcg ctgatgcgcc tgttgccggg cttattgcac
      961 cagcggtgtg cctgctgcgt tcaaatccac tcctgtaatt tttgcctggc ccgccagcac
     1021 cgaaatatac tgtcgcattg cgcgctcttc gacgctcgcc ctgagccgct cggcgatctg
     1081 tccgtgcacc gcctcgaacg gcaaggcctt gccgggaatg ctccggtcta cggccacaat
     1141 gtgaaatcca tagcgcgtcc tgaccagatc acggaggact cccgacgctc caaaccgaaa
     1201 gagcgccttt tcaaattccg gcacggtgtc gccgcggccg atctgaccga gattgccgcc
     1261 gtgttgtccc gacggacagt tggaaagctc acgcgcgagg gcatcgaacc gggcgggttc
     1321 gcgcagcaag tcattcaacg tctgctcggc gcgagcgcgc atttcgggaa cgcgcaccga
     1381 cggggtgacc tgaaacaaaa tgtgccgcgc gtgcacgaga tcgccgctct ggaactcacg
     1441 cggatgactt tcgtagtaac ggcggcattc ttcctcggtt ggcgaaggcg taacaacctc
     1501 ccgcgccagc aaggcctcaa tcgccttgtc gactgccgtc ccttccttaa ctgcggcgcc
     1561 gagcagtcca acggcaatgg cacgctgccg cagaagttcg cgcgccgcag tgagttcctc
     1621 ggacgcgccg gactcgacgg aaatttcgat gtcgttgacc gagacggtca tgaccgtacc
     1681 ggctcctttg cccgcgtccg cggcgtgcgg acaatctgat agggccggaa cagataggcg
     1741 agtgccccgg cgccgctcca gatgtggatc atgcgcgtga agggcgacac caggaagagc
     1801 gtaaagccaa gaaggatgtg caatttgtat gtcaacggca cgtcgatcac gagggcagat
     1861 gcgcttccat cgagcgtcac gatgcctttg acgtagctgg tcagcatctc gaacagggcg
     1921 ccgtccatat ggaatgccga gaggggaaca gtgaggaggc cgagcatgag ctgcagccac
     1981 aacatcagcg cgaccgcgat atcccacttg cggctgttga gccggatgcg cggatctccg
     2041 agccgccgca cgagcagaat ggtaagcccg acgatcgcga caaggccggc gatgccgcca
     2101 accaccatgg caagaagctg atgggccacc ggcgagagcg cccagtcgat ggtccaatgc
     2161 ggcgccagaa aaccggcgaa atgaccgagg atgacgatga tcaccccgta gtgaaacagg
     2221 ttcgatccga gccgcaattg ccgcttgcgc agcatttgcg atgaatcact cttccatgtg
     2281 tacggctcgc gatcgaatcg aatcaaactg ccgatcagga ggatcgagag acagagataa
     2341 ggatagatgc cgaagagaaa tgtgttcacg tagctattgg acaacatcgt caattcctcc
     2401 agtctacgcc gccggcgtac cgtcgccctg gccggccgag ccgggagcgc cggcaaaagc
     2461 aggttgctcg gcccaatcgc ggtcgagcgc ttcggtccgt tccttgaccg gcggcacctt
     2521 ggcgacatcg atggggcttt cgccggcgat gacgatcaga gcctgcagga cggcggcgta
     2581 gcggcttcgg cgcgcgacca gagaacggcc gatcgtggtc aggatgtggg cgcagtcggc
     2641 aagcatctcg cgcgcctcgg cgaggtcgcg gcagctcaga tattccagca cgacgggaag
     2701 ataatcgggc agttcgcgcg ccgacagttc aaatcccgcc ttttcgtaaa gctgccggag
     2761 ctcgaccata gcctcgccac ggtcacggct ctcgccatgc agatgctcga acagatgcaa
     2821 cgagagagct cggccacggt caaacagatc gacgtagcgt tcctcggcgg tcagcaggtc
     2881 gagaccgccg agctcgtcga tcagatcgag aaggccgccc cgctggtcgg gcgcaacgag
     2941 cggcgcgctg cggatgacgt cggcgatagc cggcagcgcc tgtcgtatct cttcggatgg
     3001 ataggacagc aaggcgctta aagcccggaa caggatcata attacacctt atgtatccaa
     3061 tggccgcatt gggctcagct tccgccgtcc catcttgccg ccgaacagtg aggagtgcgg
     3121 cggcccgaag tcgcagccat tgccgaacgt gaagccgcag gaccccttca gtccgtaagc
     3181 gtcctccgcc tcttcgcgat gactggtcgg gatgacgaaa cgatcctcgt agttggcgat
     3241 cgccagatag cgatgcatat cctccacctg tgccacggta agccccgcct gcgccaggac
     3301 agagcggcca tcaccctcgc cgagctggcg ctgacggaag tagatgcgca tcgccagcat
     3361 gcgctccagc gccgtcacga tcggagcctc ggcgcctgcc gtcagcaggt tggccagata
     3421 gcgcaccggg atgcgaagcg agcgtacgtc ggggatgtcg ccgacgctct cgatcgcgcc
     3481 ggcattggca tgcgactgga tgggcgacag cggcggcacg taccagacca tcggcagggt
     3541 acggtattcc ggatggagcg gaaatgcgat cttccaatcg accgccatct tgtaggcggg
     3601 cgagcgtttc gccgccgcaa gccaggcgtc gggcacgccg tcggcgctgg cctgctcgac
     3661 aactgccgga tcgttgggat caaggaacag tttcaactgg gcttcgtaga ggtcctgctc
     3721 gtccgccact gccgcagcgg attcaatgcc atcagcgtca tacagcagca cgccgagata
     3781 acggatgcgg ccgacgcagg tctccgagca gacggtcggc tggccgtcct cgatgcgggg
     3841 gtagcagaag atgcacttct cggctttgcc tgaggtccag ttataataaa tcttcttgta
     3901 cgggcagcct gacacgcaca tgcgccagcc gcggcacttg tcctgatcga tcagaacgat
     3961 gccatcctct tcgcgcttgt agatagaccc ggaaggacag gaggcgacgc agcttggatt
     4021 gaggcagtgc tcgcaaagcc gcggcagata catcatgaaa gtgttttcga actggccgta
     4081 gatgtccttc tgcacggctt cgaaattgta gtcttgcgaa cgcttgtcga attcgccgcc
     4141 gagaatttcc tcccagttcg ggccccactc gattttttcc atcggccgtc cggtgacgag
     4201 cgacagtggc cgcgccaccg gtgaggtcgg cagctccggc gcgatgtgca ggtgctcgta
     4261 gtcgaaggtg aacggctcgt agtagtcgtc aatttcgggc agatgcgggt ttgcgaaaat
     4321 cttcgcgaga attgcgaggc ggccgccctg cttcggcacg atcttgccgc tcttcctgcg
     4381 ctcccatccg ccgcgccagc gctcctggtt ctcccaatcc ttcggatagc cgatacccgg
     4441 ctttgtctcg acgttgttga accacgcata ttccatgccc tgacgcgagg tccacacgtt
     4501 cttgcaggtg acggaacagg tgtgacagcc gatgcatttg tcgaggttca acaccatcgc
     4561 tatttgagca cggactttca tgatgcggtc tcctcgacag cacgatcgac aggattggag
     4621 tgatcaagcc agtcgacctt gttcagcttg cgcacgatga caaactcgtc acggttggaa
     4681 ccaacggtgc cgtaatagtt gaagccgtag gagagttgag catagccgcc gaccatatgc
     4741 gtcggcttga gcatcgtgcg cgtgaccgag ttgtggatgc cgccgcgctg cccggtgatt
     4801 tgcgagcccg ggacattcac gatcttctcc tgcgcgtgat acatgagaca catgccctca
     4861 tggacgcgct ggctgacaac ggcgcgtgcc gcgatcgcgc cgttgaggtt gaagagctcg
     4921 atccagtcat tgtcgacgat gccggctttc ttcgcgtcgg tctccgaaat ccacacaatt
     4981 ggaccaccgc gcgacagagt cagcatcagc aggttgtcgg tatatgtcga atgaatgccc
     5041 catttctgat gcggcgtgat gaagttgaga acaatttcgg cttcgccgtt gctgcgcttg
     5101 ccgagcatct cggcaacggt gcgcgtatcg atgggcggcc ggtacaccgc gagaccctcg
     5161 ccgaaatcgc gaaaccagcg gtggtcctga tagaactgct gacggccgct cagcgtgcgc
     5221 cagggaatca actcattcac gttggtatag ccggcggtat agctgacatg ctcgctctcg
     5281 ataccggacc aggtgggcga tgagatgatt ttgcgcggct gcgcctgcag atcgcgaaat
     5341 ctgatcttct catcctcgcg catatcggcg agatgcttgt ggtcgcgtcc ggtgattttc
     5401 cccaacgcct cccaggcctt gaccgcaacc tcgccgttgg tttccggcgc caggtagagg
     5461 atgacttccg cggcatcaat gtcggtttcg atacggggac gcccgtcggt cgccccgccg
     5521 tcgttcacac ggtagttgag atcgccgaga cccttgacct cgtccagggt attccaggcg
     5581 atgccttttc cgccattgcc gagcttgtcg gcgagcggcc cgagcgccgt gaaccgacga
     5641 taggtgttgg gatagtcgcg ctcgacaacc gcgatttgcg gtgccgtgac gccggggacg
     5701 agttcacatt cgcccttctt ccagtctttg acatcgagtg gttgcgcaag ctcggccgcc
     5761 gtgtcgtgct ggatcggcgt gagcacgacg tcgcgttcaa tgccaagatg accggcggcg
     5821 agttccgaga atttccgcgc aatagtcttg taagtttccc agtcgctgcg cgactcccag
     5881 acgggatcca ccgccgtcga cagcggatgg atgaacgggt gcatatccga ggtgttgagg
     5941 tcattcttct cgtaccaggt cgcggtcggc agcacgatgt cggaataaag acaactcgtc
     6001 gacatgcgga agtcgagcgt gacgacgaga tcaagcttgc cttccggcgc gggatcgcgc
     6061 caggcgacct cctgtggctt cacgccatcc atctcgccga gatccttgcc ttgcaagcca
     6121 tggcgggtgc cgaggaaatg gcgcaggaaa tattcgtgcc ccttgcccga tgagccgaac
     6181 agattcgatc gccagataaa gagattgcgc ggaaaattca ccggatcgtc cggatcttcg
     6241 caagacatcc tgatccggcc ggttttcagc ccggcgacga catgatcttt gaccgcgacg
     6301 ccggcacgat ctgcatcagc cgccagtgtc agcgggttga cggcaagttg cggtgccgac
     6361 ggcagccagc ccattcgttc cgcgcgcgcg ttaagatcga tcatacttgc gcgatagggc
     6421 gccggatcgg ccagcggcga cagcagttcg gacacttcca gcttctcgta acgccactgg
     6481 tcgctgtggg catagaagaa cgatgtgccg ttcatctggc gcggcggccg cacccagtcg
     6541 agcgcgaagg ccagcatggt ccagccggtc tgcgggcgca gcttctcctg gccgacatag
     6601 tgggcccacc cgccccccga gacaccaatg gtgccgcaca tcatcaggaa gttgatgatg
     6661 ctacggtagc ccatgtcctg gtgataccag tggttcatgc ctgcgccgat gatcaccatc
     6721 gagcgccctt ttgttttctc cgcgttctcg gcgaagccac gtgccactgc gatcgccttg
     6781 tcggccggca cgccggtgat cggctcctgc caagccggcg tatagggaac gtcatcgtcg
     6841 aacgacgccg cgcaggcgcc gccgagaccc cggtcgagtc cgtaattcgc gcagagcaga
     6901 tcaaatacag ttgcgacgtg cgcctcgcca tcggagaggg caatccgccg taccgggaca
     6961 ttgcgcagca gcacatcgcc tccctggtcg ttctccggga agtgctcgtg cggcttgccg
     7021 ccgaagtagg gaaatgcgac gggcaccacg tcatcacgac ggtcgatcaa actcagtgca
     7081 agacgcacgt cttcaccggt ctcaccgtcc tttggttcaa gattccatcg ccccttcgct
     7141 tcgccgtcgg ccggccagcg gtacccgatc gacccctgcg gcacgacgag gttgccggag
     7201 tcttcgtccg tggcgacggt cttccatgcg gcacgatccg atttcggcgc accgacaagg
     7261 tcgctttgac gcacatagcg atccggcacc cagcgatcgc gctcctggcg cagccgcacc
     7321 aacatcggca tgtcggtgta acggcgcgta tagtcctgaa aataaggcac ctgccggtcg
     7381 aggaagaact cgcgcagaat cacgtggccc atggacatcg cgagcgccgc atcggtgccc
     7441 tgtttggggt gaagccaaag atcggaaagc ttggcgactt cggaataatc gggcgtgact
     7501 gccacgacct tggtgccgtt gtagcgggac tcgacgaaga aatgggcgtc cggcgtgcgc
     7561 gtctgcggta cgttcgagcc ccaggcgata atgtaaccgg aattgtacca gtcggccgat
     7621 tccggcacgt cggtttgctc gccccaggtc tgcggactcg acggcggcag atcgcaatac
     7681 cagtcgtaga aggagagaag cgagccgccg atcaggctga gatagcgcgc gccggatgcg
     7741 tatgagacca tcgacatcgc aggaatcggc gagaagccga cgacgcgatc gggtccccag
     7801 gtcttgatgg tgtggacgtt ggccgcggcg ataatctcgc tgacttcatt ccacgtcgag
     7861 cgcacaaagc cgccgcggcc acgcatggat ttgtaggacg tggctgtctt tgtgtcgccg
     7921 acgatcgccg cccaggcttc caccggtttg agcgtcttgc gcgcctcgcg ccaggccttg
     7981 atgagacggc cgcggatcat cggatatttg atccggctcg cgctgtagag ataccagcta
     8041 tacgacgcgc cgcgcgagca gccgcgcggc tcgtgattgg gcatgccggg acgtgtccgt
     8101 ggatagtccg tctgctgcgt ttcccaggtg acaacgccgc tttttacata gatcttccag
     8161 ctgcaggagc cggtgcaatt cacgccgtgc gtcgagcgca cgatcttgtc gtgct
//
LOCUS       DASSRL010000510         6536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1378250, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000510 DASSRL010000000
VERSION     DASSRL010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378250"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VFL49_10375"
     CDS             <1..132
                     /locus_tag="VFL49_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7912427.1"
                     /translation="QMPDSEKRKRADFIVDTSKGFDTARAQVREILARIATMPRRRQ"
     gene            151..852
                     /gene="dnaQ"
                     /locus_tag="VFL49_10380"
     CDS             151..852
                     /gene="dnaQ"
                     /locus_tag="VFL49_10380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HET7912428.1"
                     /translation="MREIVLDTETTGLDPVQGHRLVELGCIELLNGIPTGATFHAYVN
                     PERDVPAEAFAVHGLSAEFLKGHKRFAEIADDFLAFIGDAPLVIHNAGFDHAFLCAEL
                     KRAERELIARERLVDTLLLARRKHPAGPNRLDDLCARYGIDNSRRTKHGALLDAEILA
                     EVYVELIDARQAQLGLTESSERPLNGHEGAIIVRPRPEPLAPRLTGVELAAHREFVAS
                     LGENALWGYYQPAGI"
     gene            complement(854..1360)
                     /gene="secB"
                     /locus_tag="VFL49_10385"
     CDS             complement(854..1360)
                     /gene="secB"
                     /locus_tag="VFL49_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET7912429.1"
                     /translation="MTTTNGGPGQSGSAPGAGQDQPQLTVVAQYIKDFSFENPNAPQS
                     LNVRQEQPQIGIQINVGATPLSETDIEVVIKLDGKAETAATLLFRFELEFAGIFRVRN
                     VPQESLNPLIMIECPRLLFPFAREVIATAVRNGGFPPLLLDPVDFVSLYRQKMAQMQP
                     TPAPAAGS"
     gene            complement(1441..1887)
                     /locus_tag="VFL49_10390"
     CDS             complement(1441..1887)
                     /locus_tag="VFL49_10390"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HET7912430.1"
                     /translation="MNVAKWVILAVLALPILELAAFIAVAATIGFGWALSLILAGSLC
                     GALILRRAGSGHVARIRVALDHGSFAALQTDARGGLTLLAGILLLIPGFITDAVALLV
                     LLGALRRGFVGAPPRQHDGVVDLAPEQWHQVPEPTLPDRREDDRDR"
     gene            2094..2810
                     /locus_tag="VFL49_10395"
     CDS             2094..2810
                     /locus_tag="VFL49_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HET7912431.1"
                     /translation="MRDVFDIYTIIFLALAVFIFLRLRSVLGQRTGRERPPYDPYSAR
                     EPVRPTPEKVVALPNRTTEAVPPKPTDPVESPSDRWKGVAEAGSVIAGGLDAIVAADS
                     NFDAKHFLTGARAAYEMIVNAYAEGDRRSLKNLLAREVYDGFEGAIAEREKRGETVES
                     RFVSIDNADITAAELRGRNAQVTVRFQSKLVSVTRDKDGKVIDGNAEKVTDITDVWTF
                     ARDVSSRDPNWKLIATEAGQ"
     gene            2893..4074
                     /locus_tag="VFL49_10400"
     CDS             2893..4074
                     /locus_tag="VFL49_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HET7912432.1"
                     /translation="MATTVHLRHARTEAVSFSALEGWKDDDQKAAFGAFLKSCGAILN
                     GTRAMRAARPFYGALFKVCERAVAAGDLDRDRARAFFEGNFKPMRVMPAGQTQGFFTG
                     YYETEVEGSRFPSDKYTVPIYAAPAETVKKRRSKVFADLDRTEIEGGALAGKDLEICF
                     VKSPIDAFFAQIQGSARVKLDSGKLLRLNYVASNGMPYTPVGKFLIDRGIVSKEDMSM
                     DKIREFMEANPEEGKDLRRKNRSFVFFQETPLAAHDECIGAQGVPLTPGRSLAVDKKI
                     HIYGTPIWIDAELPIESEKPETKFRHLLVAQDTGSAIVGPARADIYFGHGEEVSHIAG
                     RIKQNGQFVMLVPQSVPAGGSAIATVNIPLPTPRPKMVVTAAARSSAATSPAQPAKRR
                     H"
     gene            4079..4618
                     /locus_tag="VFL49_10405"
     CDS             4079..4618
                     /locus_tag="VFL49_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7912433.1"
                     /translation="MTSGDGKRRRVLSAEERVLWTAVTKSIAPLRESNRSEDNELTEH
                     FVLQGRTPWQKTAPVLAAGKPPPLAPLGRRARARVARGKEAIDGRLDLHGLTQNEAHA
                     ALFNFLRTASARDARLVLVITGKGGRADGERGVLKRQVPQWLNLPEFRPLVIGFEDAH
                     IAHGGEGALYVRVRRKKRG"
     gene            4640..4816
                     /locus_tag="VFL49_10410"
     CDS             4640..4816
                     /locus_tag="VFL49_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912434.1"
                     /translation="MPKKPTVDKKKSRDQRGKSDRKRLERNLELGLEDTFPASDPVAI
                     TEPVIDVVKGDRKK"
     gene            complement(4827..6134)
                     /gene="hslU"
                     /locus_tag="VFL49_10415"
     CDS             complement(4827..6134)
                     /gene="hslU"
                     /locus_tag="VFL49_10415"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET7912435.1"
                     /translation="MTDFSPREIVSELDRYIVGQGDAKRAVAIALRNRWRRLQLDERL
                     REEVLPKNILMIGPTGVGKTEISRRLARLAGAPFLKVEATKFTEVGYVGRDVEQIVRD
                     LVEIGIALVRERKRKDVQARAEQSAEERVLNALVGPGSSPATRESFRKKLRSGELNDK
                     EIEIEVQAGSSGMPMFEIPGMPGAQMGAINIGDIFGKLGGGRTKTRRVTVDESHDILV
                     NEESDKLLDTDQLVQEAIKAVEQNGIVFLDEIDKIAGREGLRGADVSREGVQRDLLPL
                     IEGTTVNTKHGPVKTDHILFIASGAFHISKPSDLLPELQGRLPIRVELKALTRDDFRR
                     ILTEPEASLIKQYIALMDTEGVTLEFTEDAIDAIADIAVAVNASVENIGARRLQTVME
                     RVLDEVSFAAPDKSGEHVTIDAEYVQKHIGDLAKNADLSRFIL"
     gene            complement(6157..>6536)
                     /locus_tag="VFL49_10420"
     CDS             complement(6157..>6536)
                     /locus_tag="VFL49_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HET7912436.1"
                     /translation="IHGFILYWLTWAVLPHRPLIERFAIAVACESAWEIIENTPFIIE
                     RYRAGTISLQYYGDSIINSIADTVAMITGFVLARLLPISLVIGLAVTMELVVGYWIRD
                     NLTLNILMLVHPIDAVRSWQAGS"
BASE COUNT         1376 a         1909 c         1961 g         1290 t
ORIGIN      
        1 caaatgccgg attcggaaaa acggaaacgc gctgatttca tcgtggatac atcgaaaggt
       61 tttgacactg cccgtgcgca ggtacgtgaa attctcgcgc ggatcgctac aatgcccagg
      121 cggcgccagt gattctgtga cacaaacgaa atgcgcgaaa tcgtcctcga taccgaaaca
      181 acaggcctcg accctgtgca ggggcatcgg ctcgtcgagt taggctgcat cgagctgctc
      241 aatgggatac cgacgggggc gactttccac gcctacgtca accccgagcg cgatgtgccc
      301 gccgaggcat ttgcggtgca cggtctttcc gccgaatttc tgaaagggca caagcggttc
      361 gccgagatcg ccgacgactt tcttgctttt atcggcgatg ccccgcttgt gatccacaac
      421 gcgggatttg atcatgcttt tttgtgcgct gagctaaaac gcgccgagcg cgaattgatc
      481 gcgcgcgaaa ggctcgtcga caccctgctg ctggcgcgcc gcaagcatcc ggcaggcccc
      541 aatcgacttg acgacttgtg cgcaaggtac ggcatcgata attcacgccg gaccaagcat
      601 ggtgcgttgc tcgatgcgga gatactcgcc gaagtttacg tcgaactgat cgatgcccgg
      661 caggcgcaac tcggtcttac cgaatcgagc gaacgaccgc tgaacggcca tgagggggca
      721 atcatcgttc gcccacgtcc ggagcctttg gcgccacgat tgaccggggt cgaactcgcg
      781 gctcaccgcg agttcgtggc aagtctaggc gaaaatgcgc tgtggggcta ttaccagccg
      841 gcgggcatct gagtcagctg cccgcggccg gcgcaggcgt tggctgcatc tgagccatct
      901 tctgccgata caggctgacg aaatccaccg gatcgagcag gagcggcggg aagccgccgt
      961 ttcggaccgc ggtcgcaatg acctcgcgcg caaacgggaa cagcaagcgc ggacactcaa
     1021 tcatgatgag cggattgaga ctctcttgcg gcacattgcg aacccggaat atgccggcga
     1081 attccaattc aaatcgaaac aataacgttg cggcggtttc ggctttgcca tccagcttga
     1141 tcacgacctc aatgtcggtt tccgacaggg gggtagcgcc aacgttgatc tgaattccga
     1201 tctgcggctg ctcctggcgg acgttgagcg attgcggcgc gtttggattc tcaaacgaga
     1261 aatccttgat gtactgggca accaccgtga gctgaggctg atcctggcca gcccccggcg
     1321 ccgacccgct ttgtcccggc ccgccatttg ttgtcgtcat gaactctcct tcgcgccgcg
     1381 ccacccgctc gcgcggcggt tggctaacat agggtcattg agggaacaag gacgcgcaga
     1441 tcagcggtcg cgatcatcct cgcgacggtc gggcagggtg ggctcaggca cctgatgcca
     1501 ttgctccggc gcaagatcga ccacgccatc atgttgtcgg ggcggtgcgc cgacaaagcc
     1561 gcggcgcagc gctccgagca acaccaatag ggcaacggcg tcggtgataa accccggaat
     1621 cagcagtaaa atccccgcga gcagtgtgag gccgccacga gcgtcggtct gcagggctgc
     1681 aaaagagccg tgatccagcg cgacacggat gcgggccaca tggccgctac cggcgcggcg
     1741 caaaatcaat gcgccgcaaa gcgatccggc caatatcaag cttaacgccc acccgaagcc
     1801 gattgtcgca gcaaccgcaa tgaacgcggc aagttccaga atgggcagcg cgaggacagc
     1861 caatattacc cattttgcca cattcatcgg tgtttctctg ccagtgcgat gttggcctag
     1921 gcgtcctgat cgtctaaggt ccggctgaga gatcgatctg aattgcgccg aataccggcg
     1981 cgaggcccgg cccaaaggat tgatgcggcc aacagcggaa tagcgacggt gccagccgtt
     2041 ggcatcagat cgtacgcaaa tccggccggc gagcggccac ggtagccgaa agcatgcgag
     2101 acgtgttcga tatctatacc atcatttttc tagcactcgc ggtattcatc ttcttgcgcc
     2161 tccgcagcgt gctgggtcaa cggacaggca gggagcgacc gccgtacgat ccgtattccg
     2221 cgcgcgaacc tgtgcgaccc acgccggaga aagttgttgc gcttcccaac cgcacaacgg
     2281 aagcggttcc gccgaagccg acagatcctg tcgagagccc atcggatcga tggaagggtg
     2341 ttgccgaggc tggctcagtc attgcgggag gactcgacgc aattgtcgct gcagactcga
     2401 actttgacgc caagcatttt ctgaccggcg cgcgtgctgc ttatgaaatg atcgtcaacg
     2461 cttatgccga aggcgatcgg cgcagcctga agaatcttct tgcgcgcgaa gtctacgatg
     2521 gcttcgaggg tgcgatcgcg gagcgggaga agcgcggcga gaccgtggag agtcgtttcg
     2581 tgtcgatcga caacgcggat atcaccgctg ccgaactccg cggccgcaat gcacaggtga
     2641 cggtgcgctt ccagtccaaa cttgtttccg tcacacgcga caaggacggc aaggtcatcg
     2701 acggcaatgc cgagaaggtg accgacatca cggatgtctg gacttttgcc cgtgacgtga
     2761 gctcacgcga tccgaactgg aaattgattg ccactgaagc cgggcaatga gcgcatgccg
     2821 gaaatgcttt tccgcacact cattagattt ggttttggta tttcgataac agccgcagct
     2881 gtcgcgccgg ccttggcgac taccgttcat ttgcgtcacg ctcggaccga ggccgtcagt
     2941 ttttccgcac tcgaaggctg gaaggacgac gaccaaaagg ccgcctttgg cgcctttctc
     3001 aagagctgcg gcgccattct caacggcacc cgcgcgatgc gggcagccag gccgttctat
     3061 ggggccttgt tcaaggtttg cgagcgcgca gtagcggccg gggacctcga tcgcgaccgc
     3121 gcccgtgcct tcttcgaggg caacttcaag ccgatgcggg tcatgcccgc cggccagacc
     3181 cagggttttt tcaccggata ctacgaaacg gaagtagaag gctcgcgctt tcccagtgac
     3241 aaatataccg ttccgattta cgcggcgccg gctgaaacgg tgaaaaaacg tcggagcaaa
     3301 gtttttgccg accttgatcg caccgagatc gagggtggtg cgctcgcagg gaaggatctg
     3361 gaaatctgct tcgtcaagag ccctatcgat gccttctttg ctcagataca aggctccgcg
     3421 cgtgtgaaac tcgatagcgg aaaattgctg cgacttaatt atgtcgcgag caatggaatg
     3481 ccatacacac ccgtcggaaa attcctgatc gaccgcggaa tcgtgtcaaa agaagacatg
     3541 tcgatggata agattcgcga attcatggag gcgaatcctg aagaaggaaa agacctgcgg
     3601 cggaaaaacc gctctttcgt tttcttccag gaaacaccgt tagctgcaca cgatgaatgc
     3661 atcggtgcgc aaggcgttcc gctcacaccg ggccgttcgc tcgcggtcga caagaagatt
     3721 catatttacg gaacaccgat ctggatcgat gcggaactgc caattgaaag cgagaagccg
     3781 gaaacaaaat tccgccatct gcttgtggcg caggatacgg gatcagcgat tgtcgggccg
     3841 gcccgcgccg acatctattt cgggcatggc gaagaagtca gccatatcgc gggacgcatc
     3901 aagcaaaacg gacaattcgt aatgcttgtg ccgcaaagtg tcccggccgg cggcagtgca
     3961 atcgcgaccg tgaatattcc gcttccgaca ccgcgtccta aaatggtcgt caccgctgca
     4021 gcacggtcga gcgcggcaac ttcgccagcc cagcctgcca agcggcgtca ctagcgcgat
     4081 gacatccggc gacggcaagc ggcgccgcgt cctttctgcc gaagagcggg ttttgtggac
     4141 cgctgtcacg aaatcgatcg cgccgctgcg ggaatcgaat cgctcagaag acaacgaatt
     4201 gacggaacat ttcgttctgc aaggcagaac accttggcaa aaaacagcgc cggttttggc
     4261 ggccggcaag ccgccgcctc tcgcgccgtt ggggcgccgc gcgcgcgcgc gcgtagcgcg
     4321 cggcaaggaa gcgatcgacg gccgcctcga tttgcatggc ctgacgcaaa acgaagcgca
     4381 cgcggcgctt ttcaatttcc tgcgcaccgc gagtgcgcgc gatgcgcggc ttgttctggt
     4441 tatcaccgga aaaggcgggc gagccgacgg tgaacgagga gtgctcaagc gtcaggtgcc
     4501 gcaatggctg aacctgccgg aattccggcc gcttgttatc ggctttgagg atgcgcacat
     4561 cgcccatggc ggcgagggtg cgctctacgt gcgggtgcga agaaaaaagc ggggttaggg
     4621 gcgagctgga gtatcaggca tgcctaagaa gccgaccgtc gacaagaaaa agtcccgcga
     4681 ccagcgcgga aagtccgatc ggaagcggct tgaacgcaat ctggagctgg gtttggaaga
     4741 cacttttccg gcgtccgatc cggtggcgat cacggaaccg gttatcgatg tggtcaaggg
     4801 tgatcggaag aaatagccgt ccgcgatcaa aggatgaagc ggcttagatc cgcgttcttg
     4861 gcgagatcac cgatgtgctt ctgcacgtac tcggcatcga ttgtaacgtg ttcgccggac
     4921 ttatccggcg ctgcaaacga gacttcatcg agcactcttt ccatcactgt ctgcagccgc
     4981 cgggcgccga tattctcgac gctggcattc actgcgaccg caatatctgc gatggcgtcg
     5041 atggcatcct cggtgaattc aagcgtcacg ccctcggtat ccatgagcgc gatatattgc
     5101 ttgatcagcg aggcttcggg ctcggtcagg atgcggcgga aatcatctcg tgtgagcgcc
     5161 ttgagctcca cgcggatggg gagccgtcct tgcagttcgg gcagcaagtc cgatggcttt
     5221 gatatgtgaa acgcgccgga cgcaatgaag agtatatggt cggtcttcac cggcccgtgc
     5281 tttgtattga cggtggtgcc ttcgatcagc ggcagcaagt cgcgttgcac gccctcgcgc
     5341 gacacgtcgg cgccgcgcaa tccctcccga ccggcgatct tgtcgatctc gtcgaggaac
     5401 acgatgccgt tctgctcgac ggcctttatg gcttcctgca ccagctgatc ggtgtcgagc
     5461 agcttgtcgg attcttcgtt gaccaggatg tcgtgtgact cgtcgacggt tacgcggcgt
     5521 gttttcgtgc ggcccccgcc aagcttgccg aagatatcgc cgatattgat tgcgcccatc
     5581 tgcgcgcccg gcatgcccgg tatctcgaac atcggcatgc cactgcttcc agcctggact
     5641 tcgatctcga tttctttgtc attgagctcg ccggatcgca gctttttgcg gaaactctcg
     5701 cgcgtggcgg ggctcgagcc agggccgacc agtgcattga gcacacgttc ttccgcggat
     5761 tgctcggcgc gtgcctgcac atctttgcgt ttgcgctcgc gcacaagcgc aattccgatc
     5821 tcgaccaggt cgcgcacgat ctgctcgacg tcgcggccga catacccgac ttcggtgaat
     5881 ttggttgcct cgaccttcag aaacggcgcg ccggcaagtc gtgcgagccg gcgggaaatc
     5941 tcggtcttgc cgacgccggt cgggccgatc atcaggatat tcttcggcaa gacctcttca
     6001 cgcagccgtt cgtcgagctg caaccgccgc catcggttcc gcagtgcgat cgcaaccgcg
     6061 cgctttgcgt cgccctggcc gacaatatag cggtccagtt cggaaacaat ttcgcggggg
     6121 gagaagtcgg tcatgatcgg tgtttcgttt tcagattcag ctgccggctt gccagctgcg
     6181 gactgcatca atcggatgta ccagcatcag gatgttcaac gtaagattat cgcgaatcca
     6241 gtagccgacc accaattcca ttgtcaccgc aaggccaatc accagtgata tagggagcaa
     6301 tcgcgcaagc acaaacccgg tgatcatcgc aacggtatcg gcgatagaat tgatgatgct
     6361 gtcgccgtaa tattgcagcg agatcgtgcc cgcgcggtag cgctcgatga tgaacggagt
     6421 gttctctatg atctcccagg cagactcgca tgctacggcg atcgcaaaac gttcaatcaa
     6481 cgggcgatgc ggcagaacgg cccacgtcag ccaataaaga ataaaaccgt gaatga
//
LOCUS       DASSRL010000511         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1150853, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000511 DASSRL010000000
VERSION     DASSRL010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150853"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFL49_10425"
     CDS             <1..332
                     /locus_tag="VFL49_10425"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HET7912437.1"
                     /translation="LAASGWHSAVAWMRLMVAERRLRTEAARSRGEPVAAMGPALGLR
                     ELKWLKPVYVDDTIAYESEVIETRVSESRPNLGLLTVRSTGVNQRGELVISFISTTFV
                     ERRPVNK"
     gene            329..1519
                     /locus_tag="VFL49_10430"
     CDS             329..1519
                     /locus_tag="VFL49_10430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912438.1"
                     /translation="MTATVPATDVKQDEHRALGVACGAHALHDGYTDLVYVMLPIWQG
                     EFGLGFAALGLMKTMFSGTLASFQIPAGFLAERVGTAAVLALGTALAGLGYVFAGLSS
                     GVVTLVIALVIGGLGASTQHPLASSLIAHAFSGTRSLKALGTYNFAGDIGKMTVPAAA
                     ALLIVVLPWRQVLMLLGALGAIAAIAIFIMIPRYGDGAAPQKKNDSARPGRAVPAYAF
                     PLLLSVGVIDSATRMAFLTFLPFVLTAKGASLPTVGLALTLVFAGGAAGKLVCAFIGA
                     RIGAVATVWLTELVTALGIVALLPLPLEAAMILLPIVGIALNGTSSVLYGSVPDLVEP
                     QRRQRAFGIFYTGTIGAGAVSPAIYGLLGDAVGVPAALVVVAASVLVALPITLILRPA
                     LRQP"
     gene            1562..1747
                     /locus_tag="VFL49_10435"
     CDS             1562..1747
                     /locus_tag="VFL49_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HET7912439.1"
                     /translation="MRKKNTVARMTPKQASLLRKLAYDAYEPEAFSPELTSAEAEKRI
                     GVLQAKLKLMSEPPHTQ"
     gene            1841..>2211
                     /locus_tag="VFL49_10440"
     CDS             1841..>2211
                     /locus_tag="VFL49_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HET7912440.1"
                     /translation="MTYFRHAAVHAAISAAIVAALAAGAATANAQTTIITREPIESRT
                     VVTTEPLLTPVQRRRVYRTIVRERVVPAEPTIEYRVGARVPDYVPLYSVPQEVAVEVP
                     AIRPYKYMVVNNRVVLVDPAT"
BASE COUNT          384 a          715 c          675 g          437 t
ORIGIN      
        1 gactggccgc ctccggctgg cacagtgccg tggcctggat gcgcctgatg gtcgccgagc
       61 gccgccttcg gaccgaagcg gcgcgcagcc gtggcgagcc ggtagcagcc atgggacccg
      121 cgctgggttt gcgcgagctc aaatggctga agccggtata cgtggacgac accatcgcgt
      181 acgaaagcga ggtgattgag acgcgtgttt ccgaaagccg cccgaacctc ggcctgctca
      241 cggtccgttc gaccggcgta aaccagcgcg gcgaactcgt gatatccttc atcagcacca
      301 ccttcgtcga acgccgtccg gtgaacaagt gaccgcaacc gtcccagcca ctgatgtaaa
      361 gcaggacgaa caccgcgctc tcggcgtcgc ctgcggcgcg catgcgctgc atgacggcta
      421 caccgacctg gtctacgtga tgctgccgat ctggcagggt gaattcgggc ttggcttcgc
      481 cgcgctcgga ctcatgaaaa cgatgttctc cggcacgctt gcgagctttc aaattccggc
      541 aggctttctg gccgagcggg ttggcactgc ggccgtgctt gcacttggca ccgcactggc
      601 cggcctcggt tatgtttttg ccgggttgag cagtggcgtc gtcaccttgg tcatcgcgct
      661 cgtcatcggc ggccttggcg ccagcacaca gcatccgctg gcctcatcgc tgatcgccca
      721 cgcattctcc gggacgcggt ccctgaaggc gctcggcacc tacaattttg ccggcgacat
      781 cggaaagatg accgtgccgg ccgcggccgc cctcttgatc gtcgtgctcc cgtggcgaca
      841 ggttttgatg ttgctcggcg cattaggtgc catcgccgca atcgcgattt ttataatgat
      901 tccgcgatac ggcgatggcg cggctccgca aaaaaagaac gacagtgccc gccccggccg
      961 cgccgtcccc gcctacgcat ttccacttct cttgtccgtt ggcgtcatcg acagtgccac
     1021 ccgcatggcc tttctgacat tcctgccttt cgtgctcacg gccaaaggcg cgagcctgcc
     1081 gaccgtcggt cttgcgctga cgctggtttt tgccggcggt gcggcgggca aactggtgtg
     1141 cgcattcatc ggcgctcgca tcggcgcagt ggcgaccgtc tggctgactg aattggtgac
     1201 ggcgctcggc attgtcgcat tactgccgtt gccgctcgag gcggcgatga tcctgctgcc
     1261 gatcgtaggg atcgcgctca acggcacctc gtccgttctc tacggctcgg tgcctgatct
     1321 tgtcgagccg caacggcgcc agcgtgcatt cggcattttc tatacaggga cgattggcgc
     1381 cggcgccgtc tcgcccgcca tctatggttt gctcggcgat gcggtcggcg ttccggctgc
     1441 actcgtcgtc gtcgcggcga gtgtgctggt ggctttgccg atcaccctga tcctgcgacc
     1501 cgcgctgcga cagccataac gcgcggaggg aactgatccg catcgatgag attgtgccga
     1561 catgcgcaag aagaacaccg ttgcccgcat gacacccaag caggccagcc tgctccgcaa
     1621 gctcgcctac gacgcctatg aacccgaggc attcagcccg gaactcacca gcgcagaagc
     1681 ggaaaaacgc atcggcgtgc tgcaggcaaa acttaaactc atgagtgagc cgccacatac
     1741 acagtgaccg aaatcgtgat aggtcgtctc gcctgccagg cggaaccctc ctcgcgacct
     1801 tgcgtttgcc ggtttgaacc ggaatcgaga ggaggaactt atgacatatt ttcgccatgc
     1861 agcggtacat gcagcgatct ctgccgcgat cgtcgcggcg cttgcagccg gcgcggccac
     1921 ggcgaatgca cagaccacga tcatcacccg cgagccgatt gaatcgcgaa ctgtggtcac
     1981 caccgagcct ctgctgacgc cggttcaacg gcgcagggtc tatcggacga tcgtgcgtga
     2041 acgcgtcgtt ccggccgagc cgacaatcga atatcgtgtc ggcgcgcgcg tgcctgacta
     2101 cgtgccgctc tactcagtgc cgcaagaggt tgcggtcgaa gtgccggcga tccgcccgta
     2161 caaatacatg gtggtaaaca atcgcgttgt attggttgat ccggcaacga g
//
LOCUS       DASSRL010000512         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_976101, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000512 DASSRL010000000
VERSION     DASSRL010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976101"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFL49_10445"
     CDS             complement(<1..996)
                     /locus_tag="VFL49_10445"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7912441.1"
                     /translation="MTSDLRLKAAIAHWGPRFVANGVVLTDFEEVTGSIASYDDWCSA
                     WSKRAAHHEQLGREALEKKRLLTAGECLQRAGAYYHFASFLFVHDQPQMKAAHKKQIE
                     CRQAALAYLRPPGERVEIPYQGKTLAGILRRPAGVTKPPVVVMAVGLDSTKEEGDAYE
                     SPFLARGIATLIFEGPGQGEAQYEFAIRGDYEVPVKAVLDFVEKLDDLDANRIGMWGV
                     SLGGYYAPRAAAFHKRIKACIALGGPFNWGAAWDGLPELTREAFRVRSHCKTQDEARK
                     NAATLSLEGVAQNITCPLFIVNGRLDRIVPAKDAERLAREAKGPVELMMIEDGNHI"
     gene            complement(1025..1855)
                     /locus_tag="VFL49_10450"
     CDS             complement(1025..1855)
                     /locus_tag="VFL49_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912442.1"
                     /translation="MAESTVNVSIPLVSAPKGPPLAERVRATFVPRLLTGVGLLLLWE
                     FVVRALAPAYVAKPSTVLMAIPRVIADPAFLKATGATLTAVAEGLAIALVFGTIIGLL
                     IGRSAAFDRAIRHYVNGFYAMPMIIVLPLFSLWFGYSSATRIATIIFAAIFSIIVNVA
                     DGARSVPHEYMEVARSFRSNRLSMLIGIVLPSSMPYLLAGFRLAAGRALIGAVVAEFF
                     ISIGGLGYYILYNSRSYHHNEAFVGVLLLAAFGVSFELLVNWSTRRFMPWYRRDEKTS
                     "
     gene            complement(1859..2617)
                     /locus_tag="VFL49_10455"
     CDS             complement(1859..2617)
                     /locus_tag="VFL49_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912443.1"
                     /translation="MTNVHQAIRRYVPFWISLLVGIALWEAAGRSTSAAFMVPFSETL
                     RRLWELIVAGDFIKQLADSAILFFTGFFIALLVGTPLGMLLARVRPLRVGIEPYIMII
                     YATPLVALIPFILSMMGFGFAPKVLVVFLFAVFPVLYNTVEGARSIRPELIEVAKSFR
                     SGEWALWREVMLPYTLPYTMTGVRQAIGRALVGMVAAEFFLSSTGLGQLIMGASQNFD
                     TAGVFASIFVIGLVGVGLMRLGLAIEHHFARWRN"
     gene            complement(2694..>3545)
                     /locus_tag="VFL49_10460"
     CDS             complement(2694..>3545)
                     /locus_tag="VFL49_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912444.1"
                     /translation="KQGFFKKHGANVEIRPFDNGTAAARAVVAGDVDMAWSPTPPVIN
                     QISNADVPLVALYGMPNPDWVIAAGETGKTCKDMAGQDVGVDSINGARSVALRSMLTA
                     GCPGMKIEDVKQVALGSQSGAALIAGRLHYAVLHLDDLAEIQHQGKPLNIILAMKNTN
                     PTSHYLMLVARKDNLKANRDAFVRAVAGMIESAHFMQDPKNADAVAEVATNTGHSKEV
                     NKAALKAFLDIEFWAQKDDGLPRNKLEATAKLMKKIGAIKPDKEPVTFENLVDASVWK
                     DANAMVK"
BASE COUNT          718 a         1080 c         1098 g          649 t
ORIGIN      
        1 gatatggttg ccatcctcga tcatcatcag ttcgaccggg cctttcgctt cgcgtgcgag
       61 gcgctcggca tcctttgccg gcacgatgcg atcgagccgg ccgttcacaa tgaaaagcgg
      121 gcaggtaatg ttttgcgcga cgccttccaa tgaaagcgtg gctgcattct tgcgcgcctc
      181 gtcctgcgtc ttgcagtggc tgcgaacgcg aaacgcctcg cgcgtaagct ccggcaggcc
      241 gtcccaggcc gcgccccagt tgaacggccc acccagcgcg atgcaggcct tgatgcgctt
      301 atggaaggcg gcagcccgcg gcgcgtagta gccgccgagg cttacacccc acatgccgat
      361 gcgattggca tcgagatcat cgagcttctc gacgaagtcg agcaccgcct tcaccggcac
      421 ctcgtagtcg ccgcggatag caaactcgta ttgtgcctcg ccctgtccgg ggccttcgaa
      481 aatcaatgtg gcaatgccgc gcgcaaggaa aggtgattca taggcgtcgc cttcctcttt
      541 ggtggaatcg agaccgaccg ccatcacgac gaccggaggc ttcgtaacgc ccgccggcct
      601 gcggaggata ccggcgagcg tcttgccctg atagggaatc tcgacgcgct cgccgggcgg
      661 ccgcagatag gcaagcgcag cctgccggca ctcgatctgc tttttgtgcg cggctttcat
      721 ctgcggctga tcgtgcacga acaggaacga cgcaaagtga tagtaggcgc cggcgcgctg
      781 caggcattcg cctgcagtca gcagacgttt cttctcgagt gcttcgcggc cgagctgctc
      841 gtggtgtgcg gcgcgtttcg accaggcgct gcaccagtcg tcgtaggatg cgattgagcc
      901 ggtgacttcc tcgaaatccg tgagcacgac gccgttggcg acgaaccgcg gtccccaatg
      961 cgcaatggca gccttgagcc ggagatcgga ggtcatgcgc ctttagtcct tatgtttcca
     1021 ggggtcagct tgttttttcg tcgcgacggt accacggcat gaagcggcga gtgctccagt
     1081 tcaccaacag ctcgaaactg acgccgaagg cagcaagcag gagtacgcca acgaaggcct
     1141 cgttgtgatg gtaggagcgt gagttataga gaatgtaata gccaagcccc ccgatcgaga
     1201 tgaagaactc ggcaacgacc gcgccgatca gcgcgcgccc ggcggcgagc ctgaaaccgg
     1261 caagcagata gggcatcgac gacgggagca cgatcccgat gagcatgctc aggcgatttg
     1321 agcgaaatga gcgcgcaacc tccatgtatt cgtgcggcac agagcgggcg ccgtcagcga
     1381 cattgacaat gatcgaaaag atcgcggcaa agataatggt ggcgatgcgt gttgcgcttg
     1441 agtagccgaa ccacaatgag aacagcggca gcacgatgat catcggcatc gcgtagaagc
     1501 cgttcacgta gtggcggatc gcgcggtcga aagctgcgct gcggcctatg aggaggccga
     1561 tgatcgtgcc gaacacaagt gcgatcgcca ggccttccgc caccgccgtc agcgtcgcgc
     1621 cggtggcttt caggaatgcc ggatcggcaa tgacgcgggg aattgccatc agcacggtgc
     1681 tcggcttcgc gacataagcc ggcgccagcg cgcgcaccac gaattcccac agcagcagaa
     1741 ggccgacgcc ggtgagcaac cgcggcacga aagtagcgcg tacccgctcg gccagtggcg
     1801 gacccttcgg cgcgctgaca agcggaatag agacgttgac tgtggattca gccacggcct
     1861 agtttctcca gcgcgcgaag tgatgctcga tcgccagtcc cagccgcatt aatccgacgc
     1921 cgaccaggcc gatgacaaaa atcgaagcga atacgccggc ggtgtcgaaa ttctgcgacg
     1981 cgcccatgat cagctgaccg agtccggtcg agctcaggaa gaattcggcg gcaaccatgc
     2041 cgaccaacgc gcgcccgatt gcctgacgga cgccggtcat ggtgtaaggc agtgtgtagg
     2101 gtagcatcac ctcgcgccac aacgcccact cgccggagcg gaatgacttt gcgacctcga
     2161 tcaattccgg cctgatactg cgcgcgcctt cgacggtgtt gtagagcacg ggaaacactg
     2221 cgaacagaaa gaccaccagc accttgggtg caaaaccgaa gcccatcatc gacaggatga
     2281 acgggatcaa cgcaaccagc ggcgtcgcgt aaatgatcat gatgtagggc tcgatgccga
     2341 cccgcagggg gcgaacccgt gcgagcagca tgccgagcgg cgtgccgacc agcagtgcaa
     2401 tgaagaaacc ggtaaaaaac agaatcgccg agtcggccaa ctgtttgatg aagtctccgg
     2461 caacgatcag ctcccatagc ctgcgaaggg tctcgctgaa cggcaccatg aaggccgcgc
     2521 tggtgctgcg gccagcggcc tcccagagcg cgatcccgac gaggaggctg atccagaacg
     2581 gaacgtagcg gcggatggct tggtgcacgt ttgtcacttg actgtctgtc gtccctcgcc
     2641 tgcgcgggag acaacaggtc tgcaattcac ggcgccgtgc aacggcccga ggcctacttc
     2701 accatcgcat tggcatcttt ccagacgctc gcatcgacga gattctcgaa cgtcaccggc
     2761 tccttgtccg gcttgatggc accgatcttc ttcatcagct tggcagtggc ttcaagcttg
     2821 ttgcgcggca ggccgtcgtc tttctgcgcc cagaactcga tgtcgagaaa cgccttcagc
     2881 gcggccttgt tcacttcttt actgtggcca gtattggtgg cgacctcggc aacggcgtcg
     2941 gcatttttgg gatcttgcat gaaatgcgcc gactcgatca tgccggccac cgcgcgcaca
     3001 aaggcatcgc ggttggcttt caggttgtcc ttgcgcgcga ccagcatgag gtagtggctg
     3061 gtcggattgg tgttcttcat cgccaggatg atattgagcg gcttgccctg atgctggatc
     3121 tcggcaagat cgtcgagatg caggaccgcg taatgcaggc ggccagcgat tagcgccgcg
     3181 cccgattgcg agccgagcgc cacctgcttg acgtcctcga tcttcatgcc ggggcagccg
     3241 gccgtgagca tcgagcgaag ggcaaccgag cgcgcgccat tgatcgaatc gacaccgaca
     3301 tcctggccgg ccatgtcctt gcatgtcttg cccgtttcgc cggcggcaat gacccagtcc
     3361 ggattcggca tgccataaag cgcgaccagc ggcacgtccg cattcgaaat ttgattgatg
     3421 acaggcggcg tcggcgacca ggccatgtcg acatcgccgg cgacgactgc gcgcgccgct
     3481 gcggtaccgt tatcaaacgg gcggatttcc acgttggcgc cgtgcttttt gaagaaaccc
     3541 tgctt
//
LOCUS       DASSRL010000513         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_800765, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000513 DASSRL010000000
VERSION     DASSRL010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800765"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(541..1851)
                     /locus_tag="VFL49_10465"
     CDS             complement(541..1851)
                     /locus_tag="VFL49_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HET7912445.1"
                     /translation="MKFLDHPDTREAIRRYTIAGGAVVLFLTGGVGVWASTMHIAGAL
                     IAPGTIVVDSNVKKVQHPTGGVVGEVRVRDGDRVQAGDLLVRLDDTVARANLAIVTKG
                     LTELQARKARLAAERDRADTIKYPDELLQRVNDSDVGQVLAAERKLFELRRAARAGQK
                     AQLRERIEQLGKEINGLSSQQGSKEKEVALIERELTGVRELYQKNLVPITRVTALERD
                     ATRLDGERGQLVASIATAKGKIAELNLQVIQVDEDTSSEVAKEMRETDAKIGEFVERK
                     VAAADQLKRTDIIAPQTGTVFQSTVHTVGGVIPAGEPIMLIVPDAEKLAVEARVNPQD
                     IDKVQVGQTAVLRFAAFDARTTPEILGKVTRVSADTATDQRTGMSYYTLRVSLERDQT
                     ARLGNVKLVPGMPVDTFVQTGERTVLSYLMKPLSDQIVRAFREK"
     gene            complement(1848..>2479)
                     /locus_tag="VFL49_10470"
     CDS             complement(1848..>2479)
                     /locus_tag="VFL49_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7912446.1"
                     /translation="LARMLVGVWQPVRGKIRLDGATYDQWTPEVLGEKIGYLPQDVEL
                     IEGTIAENIARFSDDAKAEDILAAARAAGVHELIVGLPQGYETPIGEQGVLLSAGQAQ
                     RIALARALYGEPFLVVLDEPNSNLDAEGDEALTRAILGVRARNGIVIVIAHRPSAIAG
                     VDQLLVMNNGRQIEFGPKDVILAKLQRPMPVAAPAPALKVVPELEGATA"
BASE COUNT          484 a          751 c          717 g          527 t
ORIGIN      
        1 atctggccaa cgatgtcggg gagaggcatg gactttttcg gaggtagccg gccacggtgg
       61 cggaggacgt tagggttgcc ggctaacgta aatatcagct gccagccagg ctcaactcga
      121 caggtatttt tccatcgcgc atagatttaa gactgtcgac agcactccga cgtgtggagg
      181 gatggcctcg gctgaagcca caaagccggg gccgttcttc atccgagcat ccaaccggtg
      241 atggccaaaa ttgctcgccg ttcagcgccg tctaggcaga caacaagaac cggacgggta
      301 tccagagtac gatcgcgatc cagataagcg tcaggacgat gccaatcgcg acaacgctga
      361 tcggccattg cgacgccaaa tcacgcagtt tgcgaggatc gatcatattc tttgtgcgtt
      421 cggcgccacg aacgcatgta cgcgtcgacc acacctttga tcaacgaaag tcgcgataaa
      481 ccatgaatct ttaggagaat ccggctgcgt tttccgtcca gacgcgcaat cgctccggac
      541 tcatttctcg cgaaatgccc gtacgatttg atccgagaga ggtttcatca ggtaggacag
      601 cacggtgcgc tcgcctgtct gcacgaaggt atcaacaggc atgcctggca cgagtttcac
      661 attcccgagg cgggcggtct ggtcgcgctc aagcgacact ctgagcgtat agtagctcat
      721 accggtacgt tgatcggtgg ctgtatcggc agagacccga gtgaccttgc cgagaatctc
      781 gggcgtcgtg cgcgcgtcga aagccgcaaa gcgcaacact gctgtctgac caacctggac
      841 cttgtcgatg tcctgaggat taactcgcgc ctcaacagcc agtttttctg cgtccggcac
      901 gatcaacatg atgggctcgc ccgcgggaat cactccgcca actgtgtgga cggtggactg
      961 aaaaacagta ccggtctgcg gagcaatgat gtccgtgcgt ttgagttgat cggccgctgc
     1021 gaccttgcgc tccacgaact caccgatctt tgcatcggtt tcccgcatct ccttggcgac
     1081 ttcgcttgac gtatcttcat cgacctggat gacctgcaaa ttcagttcgg caattttgcc
     1141 tttagcggtg gcgatcgagg cgacaagctg accgcgctcg ccatcgagcc gggtcgcgtc
     1201 gcgctcaagt gcagtgacgc gggtgatcgg gacaagattc ttctgataaa gctcgcgcac
     1261 gccggtgagt tcacgttcga tcaatgcaac ttctttttct ttcgaacctt gttgcgaact
     1321 caggccgttg atctcttttc ccagctgctc gattcgctcg cgaagttggg ctttttgacc
     1381 ggcacgcgcc gcccgccgca gttcaaaaag cttgcgttca gcggcgagca cttgccccac
     1441 gtccgagtcg ttcacgcgct gaagcagttc gtcggggtat ttgatcgtgt ctgcgcggtc
     1501 gcgctctgcg gccaaccgcg ccttgcgcgc ctgcagttcg gtcagccctt tcgtgacgat
     1561 ggcgagattg gcgcgcgcca ccgtatcgtc cagtcgcacc aacaagtcgc ccgcctgaac
     1621 gcgatcgccg tcgcgaacac gcacctctcc gacaacgccg cccgtggggt gctgcacctt
     1681 tttgacattc gagtcgacaa cgatggtgcc tggtgcgatc aatgcgccgg cgatatgcat
     1741 ggtgcttgcc catacgccga cgcctcccgt gaggaaaagc acgaccgctc cgcccgctat
     1801 tgtatagcgg cgaatagctt cgcgtgtgtc cgggtgatcc agaaatttca tgcggtggcg
     1861 ccttcgagtt cagggacaac cttgagtgcc ggggcgggcg ccgcgaccgg catcgggcgc
     1921 tgcagctttg caagtatgac gtctttgggc ccaaactcga tttgcctgcc attgttcatg
     1981 acaaggagct ggtcgacccc ggcgatggcg cttggccgat gcgcgatcac gatgacaatt
     2041 ccattgcgcg cgcgaacgcc caaaattgcc ctggtcaagg cttcatcacc ctcagcgtcc
     2101 agatttgagt tcggctcgtc gagcacgacg agaaatggct cgccatacag cgcgcgcgcg
     2161 agcgcaatac gctgcgcctg acccgccgaa agcaggaccc cttgttcgcc gatcggtgtt
     2221 tcatagccct gcggcagacc gacgatcagc tcatggaccc cggccgcacg ggctgcggca
     2281 aggatatcct cggccttcgc atcgtccgag aagcgggcga tgttttcggc gatggtgccc
     2341 tcaatgagct cgacgtcctg cggcagatat ccgatttttt caccgagaac ttccggggtc
     2401 cattgatcat aggtcgcgcc gtccagccgg atcttgccgc gcaccggctg ccatacaccg
     2461 acgagcatcc tggcaaggc
//
LOCUS       DASSRL010000514         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1396000, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000514 DASSRL010000000
VERSION     DASSRL010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396000"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..757
                     /locus_tag="VFL49_10475"
     CDS             8..757
                     /locus_tag="VFL49_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET7912447.1"
                     /translation="MALSGCMHDQTAQQADARATMPAPPARHLYSTSPAYAQQAYEQA
                     GYKTARARNVPDQDRGFFNSEPHEPPSESKPSAAGGPYVAAPAAYASALAIIDRPYTL
                     DAGDRLRIVVFGQDGITNTYAVDASGKVNLPLIGAVSARALTNQQLAAAIASRLKQGY
                     VREPHVSVEVETYRPFFILGEVTTPGQYPYVANLTAEMAVAIAGGFAPRAYKRTVDLT
                     RRAGGQQVHTDVPLDYPVRPGDTILVKERWF"
     gene            complement(852..1508)
                     /locus_tag="VFL49_10480"
     CDS             complement(852..1508)
                     /locus_tag="VFL49_10480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7912448.1"
                     /translation="MTTTDKHSLGAYRADPAGTPKGGMVVVQEIFGVNHHIRAVCDRL
                     AALGYVAVAPAVFDRFVRNFESGYTPDEIAHARSYLGNLNWDHMMADIAAAQGDLKGA
                     GPLGVVGFCMGGTAAFLAACRIPGFSAAVSFYGGMIGKFADEKPKCPMQMHFGEKDDG
                     IPMDVVESIRKKQPQAEIYTYPDAPHGFYCDERASYRKEAGDLAWKRTQEFLAKHMKR
                     "
     gene            complement(1575..>2573)
                     /locus_tag="VFL49_10485"
     CDS             complement(1575..>2573)
                     /locus_tag="VFL49_10485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7912449.1"
                     /translation="MIPLSILDLSPVSAGSTAAQALRNSLDLARLADDLGFARYWVAE
                     HHNLPAIASSAPDIMIGQIAAVTRRMRIGSGGVMLPNHAPLMVAERFKVLEALYPGRI
                     DLGLGRAPGTDPATSYALRRRQGISEEDDFLERFNELMLLETRGFPAGHPFHNVRAMP
                     SDVALPPIYLLGSSDYSAQLAGHIGAAFSFAHHFANFDAAEATRLYRDSFKPSGAHEK
                     PYAILATHVVCADTDEEAERLAATVDLNFVRRAKGEYQPLASPEDAAQYEYTPVDKAR
                     IAQNRTRLSVGSPDTVKARLLPLIETTKADELMVTTMIFDHTARKRSYELLAKAFK"
BASE COUNT          462 a          830 c          821 g          460 t
ORIGIN      
        1 cgccgcgctg gcgctgtcgg gctgcatgca tgaccagacg gcgcaacaag ccgatgcgcg
       61 tgcgacaatg ccggcgcctc cggcccggca tctgtattcc acttcgccgg cctatgccca
      121 acaggcttac gagcaggccg gctacaagac cgcgcgcgcg cgcaatgtgc ctgaccagga
      181 tcgcggtttc ttcaactccg aaccgcatga gccgccatcg gagagcaaac cgagcgcggc
      241 cggcggcccc tatgttgcgg cgccggcggc ttatgcctcc gctctggcaa ttatcgaccg
      301 gccgtacacg ctcgacgccg gcgacaggct gcgcatcgtc gtcttcggcc aggacggcat
      361 caccaacacc tacgcggtcg atgcgagcgg caaggtcaac ctgccgctca tcggcgccgt
      421 atcagcgcgc gcgctgacca atcagcagct cgccgccgcg atcgcttcga ggctcaagca
      481 gggttacgtg cgcgagccgc atgtgagcgt cgaggtcgaa acctatcgac cgttcttcat
      541 tctcggcgag gtgacgacac ccggccagta tccgtatgtc gccaatctga ccgcggagat
      601 ggcggtcgcc atcgccggcg gttttgcgcc gcgcgcctac aagaggacgg tcgacctgac
      661 gcgccgcgca ggcggtcagc aggtccacac ggacgtgccg ctcgattatc cggtgcggcc
      721 gggggacacg attctcgtca aggaacggtg gttctgacgt tcgctgaccg ctcgtccccg
      781 cgtaagcggg gacccagagc acaatgatct ggattcccgc ttttgcggga atgagcggat
      841 gacaattatt ctcacctctt catgtgcttt gccaaaaatt cctgcgtgcg cttccacgcg
      901 aggtcgccgg cctccttgcg gtagctggcg cgctcgtcac aataaaagcc gtgcggcgcg
      961 tccgggtagg tatagatctc cgcctgcggc tgcttcttcc tgatggactc aacgacgtcc
     1021 atcggaatgc cgtcatcctt ctcgccgaag tgcatctgca tcgggcactt cggtttctcg
     1081 tcggcgaact ttccgatcat gccgccgtag aaggagaccg ccgcggagaa tccgggaata
     1141 cggcaggcgg caagaaacgc ggcggtgccg cccatgcaga agccgacaac gccgagcggc
     1201 cctgcgccct tgagatcgcc ctgcgcggca gcaatgtcgg ccatcatgtg atcccagttg
     1261 agattgccga ggtaactgcg cgcgtgcgcg atctcatccg gcgtgtagcc gctttcgaaa
     1321 ttgcgcacga agcggtcgaa cacggcgggt gcaaccgcca cgtagccgag cgcggcaagc
     1381 cggtcgcaga ctgcgcggat gtgatggttg acgccgaaaa tttcctgcac caccaccatt
     1441 ccgcctttgg gcgtgcccgc cggatcggcg cgatacgcgc cgagcgaatg cttgtcggtc
     1501 gtggtcaagt tgaactgttt acccaacata gcctcccgta atttttgccg aacgaaaacg
     1561 cggcggtttt accgttattt gaacgctttc gccagcagct catacgagcg cttccgcgcc
     1621 gtgtggtcga agatcatcgt ggtcaccatc agctcgtcgg ctttggtggt ctcgatcagc
     1681 ggcagcagcc gcgctttcac agtgtcgggc gagccgaccg aaaggcgcgt gcgattctgc
     1741 gcgatgcgcg ccttgtcgac gggagtgtat tcgtactggg cggcgtcttc cggcgacgcc
     1801 agcggctggt attcgccctt ggcgcggcgc acgaaattga gatcgacggt tgccgcaagc
     1861 cgctcggcct cctcgtcggt gtctgcgcag accacatgcg tcgccaaaat cgcatagggc
     1921 ttttcgtggg cgcccgaagg cttgaagctg tcgcgataga gccgcgtcgc ttcggccgcg
     1981 tcgaaattcg caaaatggtg cgcgaacgaa aacgccgcac cgatgtgccc ggccagttgc
     2041 gcgctgtagt ccgacgatcc gagaagatag atcggcggca gggccacatc ggagggcatt
     2101 gcgcgcacgt tgtgaaacgg atggccggcc ggaaagccgc gcgtctcaag aagcatcagt
     2161 tcgttgaagc gctcgagaaa atcatcctcc tcgctgatcc cctgccgccg gcgcagcgcg
     2221 taagacgtcg ccgggtcggt gccgggcgcc cggccgagcc cgagatcgat acgccccgga
     2281 tagagcgcct ccagcacctt gaatcgctcg gccaccatca acggcgcatg gttgggcaac
     2341 atcacgccgc ccgagccgat gcgcatgcgc ctggtcaccg ccgcgatctg gccgatcatg
     2401 atgtcggggg cagagcttgc gatcgccggc agattgtggt gctcggccac ccagtagcgg
     2461 gcgaagccga ggtcatcggc aagccgcgcg agatcgagcg agttgcgcaa ggcttgcgcg
     2521 gccgtggagc cggctgacac cggcgacagg tcgagtatcg agagcgggat cat
//
LOCUS       DASSRL010000515         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_713490, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000515 DASSRL010000000
VERSION     DASSRL010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713490"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..308)
                     /locus_tag="VFL49_10490"
     CDS             complement(75..308)
                     /locus_tag="VFL49_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912450.1"
                     /translation="MKYIAITAALLGLAAINIAGTARAEDFGVTIRTGENHDHWRHHH
                     WRGDYARARCRVVVTHRINGHGDRVTVRKKVCD"
     gene            complement(574..939)
                     /locus_tag="VFL49_10495"
     CDS             complement(574..939)
                     /locus_tag="VFL49_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912451.1"
                     /translation="MPLFSYFVVMGLTLTAALLYISGHIEPLGSPFPASQMVGVEKPF
                     KPEPERPAYTFTATNFAAASKPAADAYARADDTRKSVRRTDSQSRGGQSATAATETSR
                     VPGWGRIAQNPIAALMSIH"
     gene            complement(1014..1316)
                     /locus_tag="VFL49_10500"
     CDS             complement(1014..1316)
                     /locus_tag="VFL49_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912452.1"
                     /translation="MPDISATEHNEQTNIVTWKVISTGTAVVLAVFLVLWLARTAEGE
                     RTLIAQQNLEDMAAESRALCAKWGMPIGSPKYADCAADIEALRANHERRLAVSEPF"
     gene            1620..2546
                     /locus_tag="VFL49_10505"
     CDS             1620..2546
                     /locus_tag="VFL49_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7912453.1"
                     /translation="MAPRAYWKGFLRLSLVSCPIQLFPATSEREKVSFNQVNAETGNR
                     IRYRKVDEHSGEEVEQQNIVKAYQVEKGHYIEISDEELEAIALESKKTIEIDEFVPRS
                     EIDELFYMRPYYIVPDGDVGQEAFAVIRESIAKMDKVAIGRIVITTREHVIAMEPRGK
                     GLMGMLLRYPYEVRDEKEYFDDIPNIKVKKDMLDLAQHIIETKSGHFEPEAFEDHYES
                     ALRELIEKKSKGIKIEAPKRGEPSKVINLMDALRKSIRSEQANGRKPAKAKRGKKRIE
                     GQREMLFSIAGKKPAKEAARKSARASGRRKAG"
BASE COUNT          512 a          758 c          813 g          501 t
ORIGIN      
        1 gcgcggcata aattccggct aaagaaatgg cggccgtgat ggccgccctt ccttcgtcct
       61 tccggcgttt gtgatcagtc gcagaccttc ttgcggaccg tgacacgatc gccgtgtccg
      121 ttgatgcgat gcgtcaccac aacgcggcaa cgcgcgcggg catagtcgcc gcgccagtga
      181 tgatgccgcc agtggtcatg gttctcgccg gtacgtatcg tgacgccgaa atcttcggcc
      241 cttgcggtcc ccgcgatgtt gatggcggca aggcccagca gggcggcagt gattgcaatg
      301 tatttcatgc ttatctccca ttgcctcatg atggaagaca gaacggtttc acgcattttc
      361 ggttccagct ttgttttttt gcaattttgg ttccgcgcct gacccttttg cggattgtgc
      421 ccgcaacgcg aagcgctcgg ctgcgttatt ttgcggaccg tggaatctcc cctccgtcgc
      481 agaagggcgc ggtctaaggg ccgggcatgt ccccttgccc ggcccttctt tctgcgcgcc
      541 ttttcctgag ggcactttgg cggttgccgg cgatcagtga atgctcatca gcgcggcgat
      601 ggggttttgc gcgatgcgtc cccagcctgg aacgcggctg gtttccgtcg ccgcggtagc
      661 tgactgtccg ccccgcgatt gcgagtcggt ccggcgtacg gacttgcgcg tgtcgtccgc
      721 ccgcgcatag gcgtccgcgg ccggcttgga tgctgccgcg aaattcgtcg ctgtgaatgt
      781 gtaggcgggg cgctcaggct cgggcttgaa cggcttttct acgccgacca tctgcgacgc
      841 cgggaacggc gagccgagtg gctcgatgtg accgctgatg taaagcagag ccgccgtgag
      901 cgtcagcccc attaccacga aatacgaaaa gagaggcatt cgcatctgca caaacccgat
      961 gccaaacaac gcaagtcgcg tgccttgcgt tccagtttcc agtcaggcgg cgctcagaat
     1021 ggctccgaca ccgccaagcg gcgctcgtga ttcgcgcgca acgcctcgat gtcggccgcg
     1081 cagtctgcgt atttcgggct gccgatcggc atgccccatt tcgcgcagag ggcgcggctc
     1141 tcggccgcca tgtcctccag gttttgctgc gcgataagtg tgcgctcacc ctctgcggta
     1201 cgcgcgagcc acaggacgag gaacacggcc agcacgacgg cggtgccggt cgagatcact
     1261 ttccaggtca cgatgttggt ttgctcgtta tgctccgtcg cggaaatatc gggcattgga
     1321 cgcctcccgt atattggctg catgccggga acggtagcgc gaaggcggct cggacctgtg
     1381 tgagagaggt cacgtcgcct gcgaaagctt caggcggctc aagaaccgaa aatctcgagc
     1441 gaaaatgaca gtgcagcgga cgatcccgaa ccggatcctc gctctttctt ttggttaacc
     1501 cgatcacgaa aaagccggaa cggacgcaga acgcggccgg tgcgcaccgt gcaggatggg
     1561 tcctgagcgg atacattaag tacctcagcc gttagtcgga tgcgtcccag gagtttttca
     1621 tggccccgcg tgcgtactgg aagggttttc tgcgtctgtc gctggtttcg tgcccgatcc
     1681 agcttttccc cgcgacgagc gagcgcgaga aagtcagctt caaccaggtg aacgccgaga
     1741 ccggcaaccg catccgctac cgcaaggtcg acgagcacag cggcgaggaa gtcgagcagc
     1801 agaacatcgt caaagcctac caggtcgaga agggccacta catcgagatc agcgacgagg
     1861 agcttgaggc gatcgcgctg gaaagcaaga agacgatcga gatcgacgag ttcgtgccgc
     1921 ggtcggagat cgacgaactt ttttacatgc gcccttatta tatcgtaccg gacggagacg
     1981 tcgggcagga ggcttttgcc gttatccgcg aaagcatcgc gaagatggac aaggtcgcga
     2041 tcggccgcat tgtgatcacg acgcgcgagc atgtgatcgc gatggagccg cgcggcaagg
     2101 gcctgatggg catgctgctg cgctatcctt acgaggtgcg cgacgagaag gaatatttcg
     2161 acgatattcc caacatcaag gtcaagaaag acatgcttga tcttgcccag cacatcatcg
     2221 agacgaagtc gggtcacttc gagcccgagg ccttcgagga tcactatgaa tcggcgctgc
     2281 gcgagctgat cgagaagaaa tcgaaaggca tcaagatcga ggcgcccaag cgcggcgagc
     2341 cgtccaaggt catcaacctc atggatgcgc tgcgcaagag catccgttcc gaacaggcga
     2401 atgggcgcaa gccggcgaag gccaagcgcg gtaaaaagcg tatcgaaggc cagcgcgaga
     2461 tgctgttctc cattgcgggc aagaaaccgg cgaaagaagc ggcaaggaag tccgcgcgcg
     2521 caagcggccg tcgcaaggcg gggtgaccgc gcacacccgc tccggtcatt gcgatgaccg
     2581 ggtc
//
LOCUS       DASSRL010000516         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_958925, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000516 DASSRL010000000
VERSION     DASSRL010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958925"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFL49_10510"
     CDS             <1..298
                     /locus_tag="VFL49_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET7912454.1"
                     /translation="KRVLEAAIPQTLQDVVLIYASVTGRQQGELFEESYVKKIYPQCI
                     KGKLWSAIQVSTASSVCCVMDLVLRHAPARRGFVTQESILLKDFLANDFGVYFR"
     gene            295..1824
                     /locus_tag="VFL49_10515"
     CDS             295..1824
                     /locus_tag="VFL49_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7912455.1"
                     /translation="MSTVHAALKALGIQAVNSGGSTGKHWWSDHAAGSLIQSVNPATG
                     EAIAAVRSCAAQDYEHIAAQARETFATWRMVPAPKRGELVRLIGQALREKKDQLGTLV
                     SLEVGKIKPEGDGEVQEMIDMADFAVGQSRMLYGATMPSERPAHRMSEQWHPLGPVGV
                     ITAFNFPVAVWAWNAFLAAIAGDTVIWKPSPKAPLCAIAVQQICNQVMEQQGYSGIFS
                     LFITEQPSQAETMVRDARLPLISFTGSVPVGRHVAALVGQRLGRTLLELSGNNAVIVD
                     DSADMDLAVRAIVFGAVGTAGQRCTTTRRVLVHESCAGELLDRLVKAYKQVRIGNPLD
                     ADVLLGPLIDHVAVETYRAAIDEIKKEGGEILYGGRVLSRPGFFVEPTIVRARPQWPL
                     VQRETFAPILYVMTYRTLDEALAIQNGVPQGLSSAMFTNRLRDGERFVSAAGSDCGIA
                     NINIGTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTNTVNWGTDLPLAQGIKFG
                     S"
     gene            1884..2480
                     /locus_tag="VFL49_10520"
     CDS             1884..2480
                     /locus_tag="VFL49_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7912456.1"
                     /translation="MDTNRELDGLLERMVTEYVARNRAAKILRGLLEEAGVGFYPVVD
                     HVTLRTLDIDRRAQPFVTLGYAYDETLHYDDWYAKVYRKTGYPALFVDQAYPDARGKT
                     SIIPGWVGAFGDQIFHHVAVRVEDIDRAVARLKSNGVEFAGAVVGPAGGQLRQIFSAP
                     EMVGGQPFTVLELAERHRGYQGFLPPQADSLMKSTAPS"
BASE COUNT          486 a          772 c          820 g          470 t
ORIGIN      
        1 gaaacgggtg ctcgaagcgg cgattccaca gaccttgcag gatgtcgtgc tgatctatgc
       61 ctcggtcacc ggcaggcaac agggcgaatt gttcgaggag agctacgtca agaaaattta
      121 tccccaatgc atcaagggca agctgtggtc ggccatacag gtgagcaccg cctcgagtgt
      181 ctgctgcgtg atggacctgg tgctgcggca tgctcccgcc cgtcggggtt tcgtcacgca
      241 agaatccatt ctcttgaagg attttttggc caacgacttc ggagtctatt tccgatgagt
      301 accgtccatg cggcgctcaa agcgctgggc atccaggccg tgaattccgg cggcagcacc
      361 ggtaaacact ggtggtcgga ccacgccgcc ggatcgttga tccagtcggt caacccggcg
      421 accggcgaag ccatcgcggc cgtccggtcg tgcgcggctc aggactatga gcacattgcc
      481 gcgcaggcgc gcgagacctt cgccacatgg cgtatggtcc ctgcgccgaa gcggggcgaa
      541 ctcgtccgtc tgatcggcca ggccttgcgg gagaaaaaag accagctcgg cacgctcgtg
      601 tcgctggagg tcggaaagat caagcccgag ggcgacggag aagtgcagga gatgatcgat
      661 atggccgact ttgcggtcgg gcaatcgcgc atgctctacg gcgccaccat gccgtccgag
      721 cgaccggcgc accgcatgag cgagcagtgg catcctctcg ggccggtcgg cgtcatcacg
      781 gccttcaatt ttccggtggc cgtctgggct tggaatgcgt ttctcgccgc catcgcgggg
      841 gacacggtca tctggaaacc atcaccaaag gcgcccctat gcgccattgc cgtgcagcag
      901 atctgcaacc aggttatgga gcagcaggga tactcgggga tcttttcact gtttatcacc
      961 gaacaaccgt cccaggccga aacgatggtc cgggacgccc ggttgccgtt aatctcattc
     1021 accggctctg tccccgtcgg ccggcacgtg gccgccctgg tcggccaacg cctcggccgg
     1081 acgctcctgg aattgagcgg taacaacgcc gtcatcgtcg acgacagtgc ggacatggat
     1141 ctcgccgttc gagccattgt cttcggtgcg gtcggaaccg ccggccagcg gtgcacgacc
     1201 acccggcgtg tcctggttca cgaatcctgc gccggggagt tgctcgaccg gttggtgaag
     1261 gcctataaac aagtgcgcat cggcaacccg ctggacgcag acgtgctgct gggcccgctc
     1321 atcgatcacg tcgccgtcga gacctaccgg gctgcgatcg acgaaattaa gaaggaaggc
     1381 ggagagattt tgtacggcgg ccgcgtctta agccgccccg gctttttcgt cgagccgacg
     1441 atcgtacggg cccggccaca gtggcctctc gtgcagcgag agaccttcgc gccgattctc
     1501 tatgtgatga cctatcgaac cctcgatgaa gcgctggcca tccagaacgg cgttccgcaa
     1561 ggcttatcct cggcgatgtt cacgaatcgt ttgcgggacg gtgaacggtt tgtgtcggcg
     1621 gccggcagcg attgcggcat cgccaacatc aacatcggca cctccggtgc ggaaatcggc
     1681 ggggcgttcg gcggcgaaaa ggaaacgggc ggcggccggg aagccggctc ggacgcctgg
     1741 aaggcgtata tgcgtcggca aaccaacacg gtcaattggg gcacggacct gcctctggcg
     1801 caaggcatca agttcggctc gtagccgcac tgcgatgcgc ggctgatctc agctcgcaca
     1861 tctcagattt caaaggaagt ccgatggata caaatcggga attagacgga ttgctcgaac
     1921 ggatggtgac cgaatatgtg gcgcgcaacc gggcggccaa gatcctccga ggcctgctcg
     1981 aggaggccgg tgtgggattc tatccggtcg tcgatcacgt gacgctgcgc acgctggaca
     2041 tcgatcggcg cgcccagccg ttcgtcacgc tggggtatgc gtatgatgaa acattgcact
     2101 acgacgactg gtacgcgaag gtctatcgca aaaccggcta tcctgctctc ttcgtggatc
     2161 aggcctatcc ggacgcccgt gggaaaacca gcatcattcc gggatgggtg ggcgccttcg
     2221 gcgaccagat ctttcaccat gtcgcggtgc gcgtcgaaga tatcgaccgg gcggtcgcgc
     2281 gcctgaaaag caacggcgtg gaattcgcgg gtgccgtcgt cgggcccgcc ggcggccagc
     2341 tccgtcagat cttttcggcg ccagaaatgg tgggtggaca accgttcacc gtgctcgagc
     2401 ttgcggagcg ccatcgcggc tatcagggat tccttccgcc gcaagccgac agcctgatga
     2461 aatcgaccgc tccatcgtga acggtcaggc gggatgcgaa gccgacggcg acgccgacgt
     2521 tgcgcgcgcg tcggccgcct tgagaaaa
//
LOCUS       DASSRL010000517         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_153956, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000517 DASSRL010000000
VERSION     DASSRL010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153956"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1108
                     /locus_tag="VFL49_10525"
     CDS             119..1108
                     /locus_tag="VFL49_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET7912457.1"
                     /translation="MPRKPALTLACGDYEIIRPLLQGKVDVDGVDLTILTDMDSATRH
                     WRFLNNGEFDIAEVSASSYLAARDNDLPFRAIPVFLHRRFRHGFMFINTSKGIREPAD
                     LKGRRIGVKTMMTTAVLWMRGILQSEYGVPLNSIEWVAEIEDDVKVSLPPDIKYSCLP
                     NDKSVDTMLAEGELDAVFHSDFVKPFLAKDPRVARLFPDHKAEEMAYFKRTGIFPIMH
                     VLGLRKALADEQPWLAINLFRAFNEAKAVAMKRMGNPRIVPLAWYRNAWEEQERVLGA
                     DPWEYGLTKKNRKVLETLVGYSHEQGLIKKKPPLEQLFLNVDQGRKRGGEYRI"
     gene            1182..2150
                     /locus_tag="VFL49_10530"
     CDS             1182..2150
                     /locus_tag="VFL49_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912458.1"
                     /translation="MHKTFSLAAIALAVGLTGAQAQEKVWKHGILEAKSDAGFIAMVD
                     KGGFAAKRGLKVELLQIKAGATLMKALLSGEIDSVDMGAAESIVAGARGTGVKIVGCG
                     WPGVPQVVLAKTEIKSLADLKGKTVAISSPGSLPDLLFRGMLNEANVPFADVKLATQG
                     ADLDRYKSLVAGITDAAVVSNEFEAVMPPNIKVLAKGSSAVPNFLRLCIATSSKVLSE
                     RREDLVKFVAAEMDAYKYALANRAETIKIAHEMTRAKPEDKRAEFITDEAIKNRQIDP
                     TLSIPLDRLDWMQNLFLKAGVIKQTVPIESIVDKSVNADAARIAGK"
     gene            complement(2163..>2590)
                     /locus_tag="VFL49_10535"
     CDS             complement(2163..>2590)
                     /locus_tag="VFL49_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912459.1"
                     /translation="FVSLGTGIGLAVSTPYSGPGAATMTIAGAVWLVFSETAAFATGG
                     YLAARLRIRDHTPGPETKFRDAAHGFMVWVVGVGLMIAAVLATNLLSAHVEATTPALT
                     DEVRKAVAYLSFWSFMALLFGAVAATLAGILGGELRDEM"
BASE COUNT          556 a          784 c          807 g          443 t
ORIGIN      
        1 cgcttgtcta ttccaccgtc atgcccggct ttatgccggg catccacgtc tgtctgtggt
       61 caaccgagac gtggatggcc gggacaagcc cggccatgac ggataagttg gggcttgcat
      121 gccaagaaaa cctgctctca cactcgcctg cggtgattac gagatcatcc ggcctctgct
      181 gcagggcaag gtagacgtcg acggcgtcga tctcacgatt ctcaccgaca tggactcggc
      241 gacgcggcat tggcgcttcc tcaacaacgg cgagttcgac atcgcggagg tctcggcctc
      301 gtcctatctc gccgcgcgcg acaacgacct gccatttcgt gccattccgg tgttcctgca
      361 tcgccgcttc cgccacggct tcatgttcat caatacgtcg aagggcatca gggagccggc
      421 cgacctgaaa ggccgccgga tcggcgtaaa gacaatgatg acaacggcgg tgctgtggat
      481 gcgcggcatc ctgcagagcg aatacggcgt gccgctcaac agcatcgaat gggtcgcgga
      541 gatcgaggac gacgtcaaag tcagtctgcc gcccgacatc aaatactcct gcctgccaaa
      601 cgacaagtcg gtcgacacga tgctggccga gggcgaactc gacgcggtgt ttcattccga
      661 tttcgtcaag ccattccttg ccaaagaccc gcgcgtggcg cgtcttttcc cggatcataa
      721 ggcggaggaa atggcctatt tcaagcgcac cggaatcttt ccgatcatgc acgtgcttgg
      781 actgcgcaag gcgctcgccg atgagcagcc gtggctcgcc atcaatctgt tcagggcctt
      841 caacgaagca aaggcggtcg cgatgaagcg catggggaac ccgcgcattg taccgctcgc
      901 ctggtaccgc aacgcctggg aagagcagga gcgtgtgctc ggcgccgatc cgtgggagta
      961 cggactcacg aagaaaaacc ggaaggtgct cgagacgctg gtgggctatt cgcacgagca
     1021 gggcctgatc aaaaagaagc cgccgctgga gcagttgttt ctcaacgtcg accaaggtcg
     1081 caaacgcggc ggtgagtaca gaatctagcg gatattgcac ccgaataccg aaggacgtag
     1141 cctccgcgcc aaacaacaac acttatcggg aggagttatc cgtgcataag acattttcat
     1201 tggccgccat cgcgctcgct gtcggcctta ccggcgcgca agcgcaggag aaagtctgga
     1261 aacacggaat ccttgaagcc aagagcgatg ccggcttcat cgccatggtc gataaaggcg
     1321 gcttcgccgc aaagcgcggg ctgaaagtcg agctcttgca gatcaaggcc ggcgccacgc
     1381 tgatgaaggc gttgctctcg ggcgagatcg acagtgtcga catgggtgca gcggaatcga
     1441 tagttgccgg cgcgcgcggt accggcgtga agattgtcgg ctgcggctgg cctggggtgc
     1501 cgcaggtcgt ccttgccaag accgagatca agtcgttggc cgatctcaag ggcaagactg
     1561 tcgcgatttc ctcgcccggc tcgctgcccg atctgttgtt ccgcgggatg ctcaacgagg
     1621 ccaatgtgcc gttcgcggac gtcaagctgg cgacacaggg cgccgacctc gatcgttaca
     1681 agtcactggt cgcgggtatc accgatgccg ccgtggtgtc gaacgagttt gaggctgtga
     1741 tgccgcctaa tatcaaggtg ctggccaagg gcagcagcgc ggtgccgaac ttcctccggc
     1801 tttgcatcgc caccagcagc aaggtgctga gcgagcgccg cgaagacctg gtaaaattcg
     1861 tcgccgccga gatggacgcc tacaaatacg cgctcgcgaa ccgcgccgag acgatcaaga
     1921 tcgcgcacga gatgacccgc gcgaagccgg aggacaagcg cgcggaattc attaccgacg
     1981 aggccatcaa gaacaggcag atcgacccga cgctgtcgat cccgctcgac cgtctcgact
     2041 ggatgcagaa cctgtttctg aaagccggcg tgatcaagca gacggtgccg atcgagtcga
     2101 tcgtcgacaa gagcgtcaac gccgacgccg ccaggattgc aggaaaatag cgacgagcgt
     2161 actcacatct cgtcgcgcag ctcgcctccg agaatgccgg caagcgttgc agccacggcg
     2221 ccgaacaaca gcgccatgaa tgaccagaac gacaggtagg cgacagcctt gcgcacctcg
     2281 tcggtcagcg ccggcgtcgt tgcctcgaca tgagccgaca ggaggttggt ggcaaggacg
     2341 gcggcgatca tcaggcccac gccgacaacc cagaccatga aaccgtgcgc ggcgtcgcga
     2401 aacttggtct cggggcctgg ggtgtggtcg cgaatgcgga gccgggctgc caggtaaccg
     2461 ccggtcgcaa aggcggcagt ttcggaaaag accagccaaa ccgcgccggc gatcgtcatc
     2521 gttgccgcgc ccggccccga atagggcgtc gataccgcaa gtccgatgcc ggtgccgagc
     2581 gacacaaaaa
//
LOCUS       DASSRL010000518         6768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1238726, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000518 DASSRL010000000
VERSION     DASSRL010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238726"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFL49_10540"
     CDS             <1..450
                     /locus_tag="VFL49_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET7912460.1"
                     /translation="DSTHAADYNARLKATLASLDQITQRVAQLKAKHAGVPVTATEPV
                     FGPMARALGLTMRNERFQLAMMNDTEPSARDVAAFESDLKERKVKLLIYNSQVSEKLT
                     ERLRNIAKRSRVPVIGVTETMPPNVKFDVWLLGELDALDKALSEPNS"
     gene            447..1271
                     /locus_tag="VFL49_10545"
     CDS             447..1271
                     /locus_tag="VFL49_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912461.1"
                     /translation="MSAVTFDHVTLELGGHAVLSDVSLGIEAGEFIGVLGPNGAGKTT
                     LMRAILGLLKPSAGRIKVFGQTPERGNPAIGYLPQLRTVLPDLRIRGRDVIAGSLNGE
                     RWGLAINNRRDRLVVEKTLDAVGARELADRPLSEMSGGERQRLLLAQALIGSPKLMLL
                     DEPLISLDARRQEIAIDVVRRVCRERKITVLFSAHELNQLLGTLDRVLYLGNGHAVLG
                     SVEEVVTAPTLSKLFGTEIHVLRADGHIFVMSRGLDVERTDHLHDHGHEHGHHHHH"
     gene            1237..2088
                     /locus_tag="VFL49_10550"
     CDS             1237..2088
                     /locus_tag="VFL49_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7912462.1"
                     /translation="MATNTVTTIITDMLAYDFMINAFAAAGIAAIVSGIVGYFLVMRG
                     QTFAGHALSHIGFAGATGAGLIGLAPIWGLIAFTLAAGIVMGALSERISNRDVAIGVV
                     LALALGFGLLFLHYHTSFATQATALLFGNVLAVDHAMIATLALLSAITLAALTFIMRP
                     LVFSSLQPELAEAKGVPLRFVSVAFLAVVALAVSVCAQIVGVLLVFTLMVGPPAAAQR
                     ITTGLWSGLFVSAVLAVCEAWLGLTVAFYTDWPVSFCIACLSALVYFACIALPKQTRP
                     VQAAPAS"
     gene            complement(2106..3092)
                     /locus_tag="VFL49_10555"
     CDS             complement(2106..3092)
                     /locus_tag="VFL49_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7912463.1"
                     /translation="MMSKRKVNRRHKIGLTVLALAATVAAGTVFAQQRAISIATGGTG
                     GVYYPLGGGLANVLSKTLPGIQATAEVTGGSVDNLKLINAGRSELAFSMVDAALDAYK
                     GQDKFKGTEVPVRTLMVLYPNQMHVVTIEGTGIEKIADLKGKRVSTGSGGSATEVMAF
                     RVIEAAGLDRDKDMKRERLGAAESVNAIKDHKIDAFFWVGGLPTSAVTDLGATPGVKL
                     KLIDHADLAAKMNAKYGDLYAASIIKAGTYPGQTTDNKNTVVWNILVANAKMSDQDAY
                     NIVKTIFDKKADLVAVHGEAKNFTLENQVKSYSPIPWHPGAVKYMTEHGVKM"
     gene            complement(3125..5323)
                     /locus_tag="VFL49_10560"
     CDS             complement(3125..5323)
                     /locus_tag="VFL49_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HET7912464.1"
                     /translation="MAEEVRAGAGSEVISDEQLRKAEEFVQQEEGAANRLSGWVGIVV
                     TGIAVAMTLFHLYAAYDIVPTIPLRYTHVAFVLLLSFLLFPVAARFRNRIQWFDVIPP
                     LLGIATIVYALVQGDDFTDRAAVPEKWDVILGAIFIVLVLEAARRTTGWVMPLVAVCF
                     ILYAYFGPDLPPPWTHRGYDVSRLVGHLFITLEGIFGVPIDVSATLIIMFTIYGAILQ
                     HSGAGKFFIDFSLAVMGGKPSSAGRAVVASSFLLGGPSGSGVATTVMIGTVAYPMLAK
                     AGFEKNSAGGLLAAGGLGAIISPPVLGAAAFLIAEFLKISYLDVIWMATIPTCLYYLS
                     LLFMVELDSLRFGAHEVVFKPEMTVWQMTRRYGFHFVSLIAVVVFMVIGYSPMLSVFY
                     ATILAFAMSALAPDTALGPRRLLIPILLIGAGAAALFTITPIGGTPFSIEFESYFALL
                     ILVLIGALAFLGLTQFGQRLVPESKKLTAALADGSIGVLSAATTCAAAGIIVGIVTLT
                     GLGLKFSAIVIDFAGGSLLLTAIYTSLVVWIIGLAVPVTASYIICAVIAAPALTKLGV
                     PDFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGDPYVTTMQAWKYTLPAFLVPFVF
                     VLDPQGVGLLMKIPQDGSVYDIIWITLKTAIGLGALAAAAQNWALRRNTQAERWLWML
                     AGLLLVFPSLLEGLAESLTGLDVPYPAPFGLALVALLLLKQKFVPAKLAA"
     gene            complement(5423..5917)
                     /locus_tag="VFL49_10565"
     CDS             complement(5423..5917)
                     /locus_tag="VFL49_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019200036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912465.1"
                     /translation="MTLAVNILLWLVVVVLAVIAATRGRILLDEGVRTGVMEFILLLP
                     RIGIGVVGAGFVAEALPNEWIAPWLGPESGLLGIVIATLGGAFTPGGPVVGFSIGAAA
                     LKSGAGAPQVIAYSTAWALYAIHRLVIWEVPMMPARVVWLRALVSLPLPFMAAALAIL
                     LGRP"
     gene            complement(5914..6486)
                     /locus_tag="VFL49_10570"
     CDS             complement(5914..6486)
                     /locus_tag="VFL49_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET7912466.1"
                     /translation="MRATLSTPPPLRRRRRAFDWSTAIIALLAFTAAATVYLRDGRQH
                     FVEVLVGDVSLFGEMLPKVLAGCLIGAFVTLLLPREMVARWVGHESGFTGLLIAAFFG
                     FLLPGGPITIYPVAGAFLVMGADAGAVVAFIVSWTLIGYTRALVWELPFFGADFVIWR
                     IVAAIPLPIIAGLLARIAIRAGFQYRDGIK"
     regulatory      6554..6630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1378 a         2131 c         2048 g         1211 t
ORIGIN      
        1 gacagcacgc atgcagccga ctacaacgcg cggctcaagg caacacttgc ctcgctcgat
       61 cagatcacac agcgggtcgc gcagttgaag gcgaaacatg ccggcgttcc ggttaccgcg
      121 actgaaccgg tgttcggacc catggcgcgg gcgctcggcc tgaccatgcg caatgaacgc
      181 ttccagctgg cgatgatgaa cgacaccgag ccgagcgccc gcgatgtggc agcgttcgaa
      241 agcgacctca aggaacgcaa ggtcaagcta ttgatctaca acagccaggt gtcggagaag
      301 ttgaccgaac ggcttcgcaa tattgccaag cggtccagag ttccggtgat cggcgtcacc
      361 gagaccatgc cgccgaatgt caaattcgat gtctggctgc ttggcgagct ggacgccctc
      421 gacaaggcat tgagcgagcc taattcgtga gtgccgtcac ttttgatcat gtgacgcttg
      481 agctcggcgg ccacgcagtg ctgtcggacg tcagcctcgg gatcgaggcc ggcgagttca
      541 tcggcgtgct tgggccgaac ggcgccggca agaccaccct gatgcgcgca attctcggcc
      601 tgctaaagcc gagcgccggc cggataaagg tcttcggaca aaccccggaa cgcggaaatc
      661 cggcaatcgg ctatctcccg cagttgcgca ccgtattgcc cgacctccgc atccgtggac
      721 gtgacgtcat tgccggctcc ctgaacggcg aacgctgggg gctcgcaatc aacaaccgcc
      781 gcgaccggct cgtggtggag aaaacgcttg atgcagtggg tgcgcgtgag ctcgcggacc
      841 gcccgctctc cgagatgtcc ggaggcgaga gacagcggct gttgcttgcg caggcgctga
      901 taggttcgcc aaagcttatg ctcctcgacg aaccgctgat cagtctcgac gcccgccgcc
      961 aggaaattgc gatcgatgtc gtacgcaggg tctgccgcga gcggaagatc accgtgctgt
     1021 tcagtgcgca cgagctcaat cagcttctcg ggacgctcga tcgcgtgctc tatcttggca
     1081 acggccacgc cgtgctcggc tcggtcgaag aggtggtgac agctcccaca ttgtcaaagc
     1141 tgttcggcac cgaaatccat gtgcttcgcg ccgacgggca tattttcgtg atgtcgcggg
     1201 gcctcgacgt cgagcgtacg gatcacctgc acgatcatgg ccacgaacac ggtcaccacc
     1261 atcatcactg acatgcttgc ctacgacttc atgatcaatg ctttcgccgc cgccgggatc
     1321 gcggcgatcg tttccggcat cgtcggctat ttcctggtga tgcgcggaca gacttttgcg
     1381 ggtcacgcgc tgtcgcatat cggctttgcc ggcgcgaccg gcgccggcct catcggtctt
     1441 gcaccgattt ggggcctcat cgccttcacg ctggccgccg gcatcgtcat gggcgctttg
     1501 agcgagcgga tcagcaatcg cgacgtggcg atcggcgtcg tacttgccct cgcattgggc
     1561 ttcggcctgt tgttcctgca ttaccacacg tcattcgcga cacaggcaac cgcactgctg
     1621 ttcggcaacg tgctcgccgt cgatcatgcg atgattgcga cgcttgcctt gctcagcgcg
     1681 ataacgctcg cggcgctcac cttcatcatg cggccactgg tgttctcgag cctgcagccg
     1741 gagctggcgg aggccaaagg cgtgccgctt cgcttcgtct ccgttgcctt ccttgccgtt
     1801 gtcgctttgg ccgtgtcggt atgcgcgcag attgtcgggg tactgctggt cttcaccttg
     1861 atggtcgggc ctccagcggc ggcacaacgc ataaccacgg gtctctggag cgggctgttt
     1921 gtttcggcgg tgcttgccgt ctgcgaggcc tggctcggtc tgacagttgc cttttacacc
     1981 gactggccgg tgagcttctg catcgcctgt ctcagtgccc tcgtctactt cgcctgtatc
     2041 gctctcccaa aacaaacgag gccggtgcag gccgcaccgg cctcgtaaag tcgcaagcgc
     2101 agacgctaca ttttcacgcc gtgctcggtc atgtatttca ccgcgcccgg atgccacgga
     2161 atcggcgaat aggattttac ctggttttcg agcgtgaaat ttttcgcctc gccatgcacc
     2221 gcgaccaggt ccgccttctt gtcgaagatc gtcttcacga tgttgtaagc gtcctgatcc
     2281 gacatcttgg cgttcgcgac aagaatattc cacaccaccg tgttcttgtt gtccgtcgtc
     2341 tgtcctggat aggtgccggc cttgatgatg ctcgcggcat ataagtcgcc gtacttggca
     2401 ttcatcttcg ccgcaagatc ggcgtgatcg atcagcttga gcttgacgcc gggcgtcgcg
     2461 ccgaggtccg tcaccgccga ggtcggcaga ccgccgaccc agaagaaggc atcgatcttg
     2521 tgatccttga ttgcattgac cgactcggcg gcaccgagtc gttcgcgctt catgtccttg
     2581 tcccggtcga gaccggccgc ttcgatcacg cggaaggcca tcacctcggt cgcgctgccg
     2641 cccgacccgg tggagacgcg cttgcccttg agatcggcta tcttctcgat gccggttcct
     2701 tcgatcgtca caacgtgcat ctggttcgga tagagcacca tcagcgtgcg gaccggcacc
     2761 tccgtgccct tgaacttgtc ctggcccttg taggcatcga gcgcggcgtc gaccatcgaa
     2821 aacgcaagtt cgctgcgtcc ggcattgatg agcttgagat tgtcgaccga gccgccggta
     2881 acttcggccg tcgcctggat cccgggcagc gttttcgaca gcacattggc aagcccgccg
     2941 ccgagcggat agtagacgcc gccggtgccg ccggtcgcga tcgaaatcgc gcgctgctgc
     3001 gcgaagactg ttccggcagc gaccgtggca gccagcgcca agacggtgag tccgatcttg
     3061 tgccgccggt ttactttgcg tttcgacatc attttctccc ttgtcgtttc tgttcttgtc
     3121 caccttatgc cgcgagtttc gctggaacga atttctgttt caatagcagg agagcgacca
     3181 aagcgagacc aaagggcgca ggatacggca cgtcaagccc cgtcaagctc tcggcaaggc
     3241 cttccagcaa gctcgggaac accagcagca ggcctgccag catccataac catcgttcgg
     3301 cctgcgtatt ccgccgcaag gcccaatttt gtgcggccgc agcaagggca cccagaccga
     3361 tggccgtttt caaagtgatc cagatgatgt cgtacaccga tccgtcctgc gggatcttca
     3421 tcagcagtcc gacaccctgc ggatcgagaa cgaaaacaaa cgggacgaga aatgccggca
     3481 gcgtgtattt ccacgcctgc atggtggtga cgtaaggatc gccaccggtg atggcagccg
     3541 ccgcaaaggg cgacaatgcg gtcggcggcg acacttccga gagcaccgca taatagaaaa
     3601 tgaacatgtg tgcggcgaaa tccggcacgc cgagtttcgt caaagcgggt gcggcgatga
     3661 ccgcacaaat gatgtaggac gcagtcaccg gcacggcgag accgatgatc cagacgacca
     3721 gcgaagtgta gattgccgtc aacagcaggc tgccgccggc aaaatcgatc acgatcgcgg
     3781 aaaacttcag cccaagccct gtcagcgtga cgatgccgac gatgattccg gcggcggcgc
     3841 aggttgttgc cgcgctcagc acgccgatcg aaccgtccgc cagggctgct gtgagtttct
     3901 ttgattccgg caccagccgc tggccgaact gcgtcaatcc aagaaaagca agcgcgccga
     3961 tcaacaccaa tatcagcagc gcgaaatagc tttcaaactc gatcgagaac ggtgtccctc
     4021 cgatcggcgt aatcgtgaac aacgccgcag cgcccgcacc gatcaggaga atggggatca
     4081 gcaggcggcg cggaccgagc gcggtatccg gagcaagcgc gctcatcgcg aaggccagaa
     4141 tcgttgcata gaaaaccgag agcatcggtg aataaccgat caccatgaag accacgacgg
     4201 cgatcagcga gacgaagtgg aagccgtacc gcctggtcat ttgccacacg gtcatctcgg
     4261 gtttgaacac cacctcgtgc gcgccgaacc gcagcgagtc gagttcgacc atgaagagca
     4321 acgacaggta gtagagacag gtcgggatgg tcgccatcca gatcacgtcg aggtagctga
     4381 tcttgagaaa ctcggcgatc aggaaggccg ccgcgccaag tacgggcggt gaaataatgg
     4441 cgccgagacc tcctgccgcg agcaaaccgc cggcggaatt tttctcgaac ccggctttcg
     4501 ccagcatcgg ataggcgacg gtgccgatca tcacggttgt tgcaacgcct gagccggaag
     4561 gacctccgag cagaaacgac gatgccacta ccgcgcggcc ggcgctcgac ggcttgccgc
     4621 ccataaccgc gagcgagaaa tcgatgaaga attttccggc gcccgagtgc tgcaggatgg
     4681 caccgtagat tgtgaacatg atgatcagcg tcgccgaaac atcaatcggc acgccgaaga
     4741 tgccctccag cgtgatgaac aggtggccga ccagtcgcga aacgtcgtaa ccgcgatgag
     4801 tccacggcgg cggaagatcg ggaccgaaat aggcgtagag aatgaagcag acggccacga
     4861 gcggcatgac ccaaccggtc gtgcgccgcg ccgcttccag caccagcacg atgaagatgg
     4921 cgccgaggat cacgtcccac ttttcgggca ccgccgcgcg gtcggtgaaa tcgtcgccct
     4981 gcacgagcgc atagacgatg gtggcaattc cgagcagcgg cggaatgaca tcgaaccact
     5041 ggatccggtt gcgaaagcgc gcagcgaccg gaaacagcag gaagctcagc agcagcacga
     5101 aggcgacgtg cgtgtaacgc aacggaatgg tcggcacgat gtcgtaggcg gcatagaggt
     5161 ggaacagcgt catcgcgacg gcgattccgg tcacgacgat gccgacccag cccgagagcc
     5221 ggtttgcggc gccttcttcc tgctgaacga attcttcggc cttgcgcagc tgctcgtcgg
     5281 agatgacctc cgagcctgcg cccgccctca cctcttccgc catcgtcccc tcgcctggca
     5341 gcggtgttgt cccgctttcc gcggcacgct agcacgagct aaaggcgggg aatacctgcg
     5401 gaaaatgcca ccttcggcgc attcagggac gcccgagcag gatggcaagc gccgcggcca
     5461 tgaacggcag cggcagcgat accaatgcgc gcagccagac tacgcgcgcc ggcatcatcg
     5521 gcacttccca gatcaccagc cggtggatcg catacagcgc ccaggcggtc gagtaggcga
     5581 tgacctgcgg cgcgccggct ccgctcttga gcgcggccgc gccgattgag aaaccgacca
     5641 ccggcccgcc cggcgtgaac gcgccgccaa gcgtcgcaat cacgatgccc aacaggcccg
     5701 actccggacc aagccacggt gcgatccatt cgttcggcag cgcttccgca acgaaccccg
     5761 cgccgacgac gccgatgccg atgcgcggca gcagcaggat gaactccatg acgcccgtgc
     5821 gcacgccctc atccagcaag atgcgtccgc gcgtggcggc gatgacggcc agaacgacga
     5881 cgaccagcca cagcaggatg ttgacggcga gcgtcatttg atcccgtcgc gatactgaaa
     5941 gccggcgcga atcgcgatgc gggcgagcag accggcaatg atcggaagcg gtatcgcagc
     6001 aacgatccgc cagatcacaa aatccgcgcc gaagaacggc aattcccaga ccagcgcgcg
     6061 tgtgtagccg atgagagtcc agctcacgat gaatgcgacc accgcgccgg catcagctcc
     6121 catgacgaga aaggcgccgg cgacaggata aatagtgata ggcccgccgg gcaggagaaa
     6181 tccgaaaaac gcggcgatca gcagcccggt gaatcccgat tcgtggccga cccaacgcgc
     6241 caccatctcg cgcggcaaca gcagcgtgac aaaggcgccg atcaggcaac cagccagcac
     6301 cttcggcagc atctcaccaa agagcgaaac gtcaccaaca agaacttcga caaaatgctg
     6361 gcgtccgtca cgcagataga ccgtggcggc ggcggtgaaa gccaggagcg caatgatcgc
     6421 cgtcgaccaa tcgaaggcgc gacggcggcg gcgtaatgga ggcggcgtgc tcaatgtggc
     6481 gcgcacacta gtcgacccgg atcgaattgt ctgccgcgtc ccggttgacc ctgcattgcg
     6541 tcaacgatac cttcgccgcg atggggatgg ggtcccccga caaccgcccg caaatcgtaa
     6601 aatcttgggg ctgatgactc ctaccgtgca acagggcggt aggactctgt ccgccgcagg
     6661 gcggtttttg tttgccctga aaaaaaggga cccgaccaat gggctttctg atcgtgtttg
     6721 tcggcggcgg catcggcgcc gcacttcgcc acgggtttaa tctcgcct
//
LOCUS       DASSRL010000519         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_661381, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000519 DASSRL010000000
VERSION     DASSRL010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661381"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFL49_10575"
     CDS             <1..735
                     /locus_tag="VFL49_10575"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7912467.1"
                     /translation="LERAITPKTKWLIFNSPSNPSGAAYSRAELKALTDVLVKHPHVW
                     ILTDDMYEHLVYDDFVFTTPAQVEPNLYDRTLTMNGMSKAYCMTGWRLGYGAGPEKLI
                     KAMGTLQSQSTSNPTSITQWAGVEALNGPQDFIPKHNKVFKERRDLVVSMLNQSNGLS
                     CPKPEGAFYVYPTCKGTIGKTAPSGKKIETDEDFAKELLATEGVAVVHGTAFGTAPAF
                     RISYATATKDLEEACSRIQRFCGSLR"
     gene            832..1302
                     /locus_tag="VFL49_10580"
     CDS             832..1302
                     /locus_tag="VFL49_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HET7912468.1"
                     /translation="MKTRLAIIAAAAGIAGLAATSVPASAAVEVGVLRCKVEPSVGYV
                     VASERWMRCAFHPNDGSRPQYYRGRAGRIGIDFGVTNDAVLVWGVFAPTRRLTRHALA
                     GTYAGASAGAAIGAGVGANALLGGSHNTIALQPLSAEGSTGLSVALGVTALSLR"
     gene            1516..>2021
                     /locus_tag="VFL49_10585"
     CDS             1516..>2021
                     /locus_tag="VFL49_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7912469.1"
                     /translation="MADAAKPTNGISSQDISSRGARCIALVGPFQSGKTSLLEAILAR
                     TGTIQRPGTVAAGNTVGDASAESRDHKMSVELSCASTDFLGDTYTFLDCPGSVEFMHE
                     MRAALPPCDAAVVVCEADERKLPQLQLVLRELEDRNIPRFLFINKIDKADKRVRETLS
                     SLQPASRVP"
BASE COUNT          383 a          639 c          620 g          379 t
ORIGIN      
        1 ctagagcgcg cgatcacgcc aaaaacgaag tggctgattt tcaattcgcc ttcgaacccg
       61 tcaggcgcgg cctattcgcg cgccgagttg aaggcgctca cggacgttct ggtgaagcat
      121 ccgcatgtct ggattctcac cgacgacatg tacgagcatc tcgtctacga cgacttcgtg
      181 ttcacgacgc cggcgcaggt cgagccgaac ctctatgacc gcacgctgac gatgaacggc
      241 atgtcgaagg catattgcat gaccggctgg cgtctcggtt acggggccgg cccggagaag
      301 ctcatcaagg caatgggcac gttgcaatcg cagtcgactt ccaacccgac gtcgatcacg
      361 cagtgggcgg gcgtcgaggc gctcaacggt ccgcaggatt ttatcccgaa gcataacaaa
      421 gtgtttaagg aacgccgtga cttggttgta tcgatgctca accagtcgaa cggcctgtcg
      481 tgtccgaagc cggaaggcgc gttttacgtg tatccgacct gcaaaggcac gatcgggaaa
      541 accgcgccgt cggggaagaa gatcgagacc gacgaggact tcgcgaagga acttttggcc
      601 accgaaggcg ttgcagtggt gcacggtacg gccttcggga ccgcgccggc ctttcgcatt
      661 tcctacgcga cggccacgaa ggatctcgaa gaggcttgca gccgcattca gcggttctgc
      721 ggctcgttac gttaagcaaa tcctcaaaga tcgtaccgct gcgccttatt tgctcccgga
      781 agttccggga gcattcgttt gtaatggatg gatcaacgat caggaggtca catgaaaacc
      841 cgtctcgcca tcatcgccgc cgctgccgga attgccggtc tcgcggccac gtccgttccg
      901 gcttcggccg cagtggaagt cggcgtcctg cgatgcaaag tggagccttc ggtcggttac
      961 gtcgtcgcgt ccgaacgctg gatgcgctgc gccttccatc cgaatgacgg aagccgcccg
     1021 caatattatc gcggccgcgc gggccgcatc ggcatcgact tcggcgtgac gaatgacgcc
     1081 gttctggtct ggggcgtctt tgcgccgacc cgccgtctca cgcgccatgc gctggccggt
     1141 acctatgccg gcgcctcggc gggcgctgcg atcggcgcgg gtgttggtgc aaacgcgctg
     1201 ctcggcggct cgcacaacac catcgcgttg cagccgcttt cggcggaagg cagcacgggt
     1261 ttgagcgtcg cgctcggggt tacggcgctt tcgctgcgct aaaatacaaa gttccacaaa
     1321 gttgatgcgg cggcccgggc aaaacccggg ccgcttttat ttttaacaat gcgcggcttt
     1381 gcatgcttgc gctcggcggc ttcccctgaa acgatagctt cccggatcgc cccatttccg
     1441 cgacccgcaa gacgctacca agccctggca ttcgcaaggg cggcgccagg gcccctctaa
     1501 cccagggagg cctcgatggc ggacgcagca aaacccacca acggcataag ttctcaggac
     1561 ataagttccc ggggggcacg atgcatcgcg ctcgtgggcc cattccagtc cggcaagacg
     1621 tcgttgcttg aagcgattct cgcgcgcacc ggcacgatcc agcgccccgg cacggtggcg
     1681 gcgggcaaca ccgtcggtga cgcgagcgca gaatcgcgcg atcacaagat gagcgtggag
     1741 ctttcctgcg cctccaccga ttttctcggc gacacctata cgttcctcga ttgccccggc
     1801 tcggtcgagt tcatgcacga aatgcgcgca gcgctgccgc cctgcgacgc ggcggtcgtg
     1861 gtatgcgagg cggatgagcg caagctcccg caactgcaac tcgtgctgcg ggagctcgaa
     1921 gaccgcaaca ttccgcgctt cctgttcatc aacaagatcg acaaggccga caaacgcgtg
     1981 cgcgagacgc tctcctcgct gcagccggca tcgcgggtgc c
//
LOCUS       DASSRL010000520         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1605991, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000520 DASSRL010000000
VERSION     DASSRL010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605991"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFL49_10590"
     CDS             <1..605
                     /locus_tag="VFL49_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7912470.1"
                     /translation="LPAPVIVRPSDTADHEPRSIDAGDIREIALLRGTLAHRLLQSLP
                     DIPAPRREKVAREFLSRRGKKLSADQSAALVREVLLLIENKDFAELFTPGSRAEVPIV
                     GNLSKGALVSGQVDRLSVTKEQVLIADFKTNRMPPRRIEDAPAAYVEQLAAYRAVLQK
                     LYPDRPIRAALIWTEVPDLMELSAELLDRALAIHVAARAP"
     gene            689..1009
                     /gene="trxA"
                     /locus_tag="VFL49_10595"
     CDS             689..1009
                     /gene="trxA"
                     /locus_tag="VFL49_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7912471.1"
                     /translation="MAVGKVSDASFESDVLKSAEPVVVDFWAEWCGPCRMIAPALEEI
                     AGQLGDKVKIVKLNVDENPNTAAKYGIMSIPTLMLFKNGEIASRQVGAAPKQKLHQWI
                     SGSV"
     gene            1070..2098
                     /locus_tag="VFL49_10600"
     CDS             1070..2098
                     /locus_tag="VFL49_10600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7912472.1"
                     /translation="MTLALPHSFAPMEALSVDEIPKGKDWQYEPKWDGFRCLVFRDGN
                     AVNLRSKSGQPLTRYFPELAAAFSVLKPKKFVLDGEIVVPQGKAFSFDQLLQRIHPAA
                     SRVSRLAAEMPAMLIVFDLLLVGDKNLTRQPLGERRKMLEDFAHEFLRGRRLIRLSPA
                     TVRLSDAKNWLKQVGATLDGIVAKRRDLPYESGNRIGMQKIKNFRSADCVVGGFRYNA
                     GKKTVGSLLLGVYNEQGLLDHVGFTSSLRVEEKKALTVRLKKLIEPPGFTGKKPGGPS
                     RWSTGRSAQWQPLKPKLVVEVCYDHFSNGRFRHGTRLLRWRPDKAPRQCTMDQLRQTK
                     SDLMTLLK"
     gene            complement(2116..>3185)
                     /locus_tag="VFL49_10605"
     CDS             complement(2116..>3185)
                     /locus_tag="VFL49_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7912473.1"
                     /translation="FRIGAPGGGYLVSDDELATTLEECERANAGAPITVFEITTAAGL
                     SIFARHPADLLLLEVGLGGRLDATNVIDSPLASIITRISIDHVDFLGNSLQQIAGEKA
                     GILKRGVPAAIASQNREVLAVIERQAAKLGVPLKIAGEDWTATEERGRLVYQDDAGLL
                     DLPAPRLYGRHQFENAGLAIAALRAAKRFSIPSAAFDAGMTKAEWPARLHRLSQGRLV
                     DSAPAGSELWLDGGHNPDGGRAIAAALADLEERVSRPLVLIVGMLASKDCEGFLRNFA
                     GLARRLIAVPVTGAEKGLAAETVAEVARTIGISATSRDHLAEAFDAVRKLDLEPPPRI
                     LITGSLYLAGEVLRENGTPPQ"
BASE COUNT          659 a          964 c          942 g          620 t
ORIGIN      
        1 cactgccggc gccggtgatc gtgcgcccat ccgatacagc ggaccatgag ccccgttcta
       61 ttgacgccgg cgacatccgg gagatcgcgc ttttgcgcgg gacgctcgcc caccggctgt
      121 tgcagtcgct gccggacatc ccggcgccac ggcgagaaaa agtggcccgc gaattcctgt
      181 cgcgaagagg caagaagtta tcggccgatc aaagcgcggc gctggttcgg gaggtattgc
      241 ttctcatcga aaacaaggat tttgccgagc tgttcacccc gggcagccga gccgaagttc
      301 cgatcgtcgg aaacctcagc aaaggggcgc tcgtctctgg tcaggtcgac cgtctttctg
      361 tgaccaaaga gcaagtgttg attgccgatt tcaagaccaa ccgcatgccg cctcgccgaa
      421 tcgaggacgc tccggccgct tacgtcgagc agctcgcggc ttatcgcgcc gtgctgcaga
      481 aactttaccc cgacagacct atccgcgccg ctctcatctg gaccgaagtt cctgatctga
      541 tggagctttc ggccgaactt ctcgaccgag ccctcgccat tcacgtcgcc gcgagagcgc
      601 cttgacgctt gcagactacg ttcataggtt cctgccctca caattatttg ggggcgattc
      661 ccccattcaa tgacaagagg gtgctgccat ggccgtcggc aaagtgtcgg acgctagctt
      721 tgagtcggat gtattgaagt ctgccgaacc ggtggtcgtg gatttctggg cagaatggtg
      781 cggcccctgc cgcatgatcg ccccggcgct ggaagagatc gccggccagc tcggcgacaa
      841 ggtcaagatc gtcaagctga acgtcgatga aaatccaaac accgcggcca agtacgggat
      901 catgtcgatc ccgacactga tgctgttcaa gaacggcgaa atcgcctcgc gccaggtcgg
      961 ggcagcaccg aaacagaaac tgcaccagtg gatcagcggt tccgtttgac agcgacaaaa
     1021 gattccaaag cggccggcgc aaagccggcc gttttgcgtt ggtaggacga tgacgctcgc
     1081 actgcctcac tcgttcgctc caatggaagc tttgtccgtt gacgagatcc cgaagggcaa
     1141 ggattggcaa tacgagccga aatgggatgg ctttcgttgt ctcgtttttc gggacggcaa
     1201 cgcggtcaat ctgcgatcga agtccggcca gccgctcacc cgctactttc ccgaactcgc
     1261 ggccgctttt tcggtgctaa agccgaaaaa gttcgtgctc gacggcgaaa ttgtcgtgcc
     1321 gcagggaaaa gcgttttcct tcgaccaact gctccagcgt attcatccgg ccgcaagtcg
     1381 ggtaagtcgc ctggccgcag agatgccggc gatgttgatc gtgtttgatc ttctgctcgt
     1441 cggcgacaag aatctgacca ggcagccgct tggcgagcgt cgcaagatgc tagaagactt
     1501 cgcgcacgaa tttttgcgcg gtcgcaggct cattcgactg tccccggcaa ccgtgcgtct
     1561 ttccgatgcg aagaattggc ttaagcaggt tggggcgacg ctcgacggca tcgtcgcgaa
     1621 gcgtcgtgat ttgccctacg aatccggcaa ccggatcgga atgcagaaga tcaagaattt
     1681 ccgctcggcc gactgcgtgg ttggcggttt ccgctacaat gcgggcaaga agacggtcgg
     1741 atcactgctg ctcggtgtct ataacgaaca agggctgctc gatcacgtcg ggttcacgtc
     1801 ctcactcagg gttgaagaaa aaaaagctct gactgtgagg ctcaaaaagc tgatcgagcc
     1861 gcctggtttc accggcaaga agccgggcgg accgagccgc tggtcgaccg ggcgttccgc
     1921 gcaatggcag ccgctcaagc cgaagcttgt ggttgaggtt tgctacgatc atttcagcaa
     1981 cgggcgattt cgccatggca cacgactcct gcgctggcgg cccgacaagg cgccgcgtca
     2041 atgcacgatg gatcagctgc gccagacaaa atccgacttg atgacgttgc tgaaataggc
     2101 tcaggcgata aaccgctatt gaggcggcgt tccgttctct cgcagcacct caccggcgag
     2161 gtagagcgaa ccggtgatca agattcgcgg tggtggctcg agatcgagct tccgcacggc
     2221 atcgaacgct tcggccagat ggtcacggct tgtcgccgaa atcccgatcg tgcgggcaac
     2281 ctcggccact gtttccgcag ccagcccttt ttcggcaccg gttaccggta ctgcgatcag
     2341 gcggcgggcg aggccggcaa aatttcgcag aaagccttcg caatccttgc tggcgagcat
     2401 accgacgatc agcaccagcg gcctcgaaac gcgctcctcc agatcggcca gggctgctgc
     2461 gatggcgcgg cctccgtccg gattgtgccc gccatccagc cacaactcac tgccggcagg
     2521 cgcggaatcg accaaccggc cttggctcag acgatgcagc cgcgcaggcc actcagcttt
     2581 tgtcatgcct gcatcgaatg cggcactcgg aatcgaaaat cgcttcgccg cacgcaaagc
     2641 ggcaatcgcc aagccggcgt tttcaaactg gtgtcgcccg tatagcctcg gagccggcag
     2701 gtcgagcaag ccagcgtcgt cttgatagac cagcctcccg cgctcctctg tcgcggtcca
     2761 atcctcgcct gcaattttaa gcggaacgcc gagcttggca gcctgccgtt caattacggc
     2821 gaggacttcg cgattctgcg aagcgatggc ggcgggaacg ccgcgcttga gtatgccggc
     2881 cttctcgccg gcaatctgct gcaaagaatt gccaagaaag tcgacatgat caatcgaaat
     2941 gcgcgtgatg atggacgcca aaggactgtc aatgacgttg gttgcatcga gccgcccacc
     3001 gaggcccacc tcaagcagaa gaaggtctgc gggatgccgc gcaaaaatcg acaggccggc
     3061 tgcagttgtg atttcaaaca ccgtgatcgg tgcacctgca ttcgcgcgct cgcattcttc
     3121 cagagttgtg gcaagctcat catcggaaac gagataaccc ccgcccggcg caccgatgcg
     3181 gaagc
//
LOCUS       DASSRL010000521         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_871412, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000521 DASSRL010000000
VERSION     DASSRL010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871412"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFL49_10610"
     CDS             complement(<1..655)
                     /locus_tag="VFL49_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7912474.1"
                     /translation="MALPAAQLNESDRHFLGVERSLTGRAWRDRLDARGSARALAIAQ
                     RHGLDELLARVLAGRNVEVDSVEEFLDPTIKRLLPDPYTLTDMQAASVRIADAVIGGE
                     SVAIFGDYDVDGATAAALLCRYLRHCGLDPIVHIPDRVFEGYGPNVDAIRSFAQRGAR
                     LLITVDCGVTSLEPLAEAKNFGLGTIIIDHHQADERLPDVVAIVDPNRADDLSGLGHL
                     "
     gene            complement(751..1782)
                     /locus_tag="VFL49_10615"
     CDS             complement(751..1782)
                     /locus_tag="VFL49_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912475.1"
                     /translation="MRHVHLVGSVPLRDAREVFATVSKALGPYLRRIPDGETGERSDW
                     ITWLEPAFAENPALEKSDELFRVHATGTARIRYRLRSGRSMRDVRFGNLFYADIAKAS
                     YADFSTLKREGIVPKDCRLQIDLVPAHSVIWLFVQEDLHRPLDPVYNEALKREIDKIA
                     GALPHDQIAIQFDVASAVFARLQRGEPNAYGKTRAEMLKSFSAILTDLADHVPRDIEL
                     LFHFCYGDSNHRHVVEPTDMGDMVDLANELFADIKRSVQLIHMPVPRNRNDDAYFEPL
                     ARLRFKPETELSLGLVHFTDGVTGTRQRLATAERHAKAFSIATECGFGRRDPKTIPEL
                     LRIHAELAG"
     gene            complement(1788..2780)
                     /gene="glpX"
                     /locus_tag="VFL49_10620"
     CDS             complement(1788..2780)
                     /gene="glpX"
                     /locus_tag="VFL49_10620"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET7912476.1"
                     /translation="MPSITVQPELLLERILSLEIVRVTERAAVSSARLRGHGKDKASD
                     QAAVDAMRRELNKLPIDGTVVIGEGEMDEAPMLFIGEKVGNKTGPKVDIAVDPLEGTT
                     LCAKNMPGAIATLAMADAGTLLHAPDCYMDKIAIGPGYPKGTIDLDAPPAENILSLAK
                     AKGVKASEITAILLDRPRHADIIAAVRKTGAAVSLISDGDVAGVIHCADAKTGIDIYL
                     GIGGAPEGVLAASALRCIGGQMQTRLIIDTEELRERTLKMGIRDPRKKYQIEDMVRGD
                     CLFAATGVTSGAMLSGVKFGKDVIETETVVMRSVTGTVRRVLAEHRQFDKFHLD"
     gene            complement(2813..4057)
                     /locus_tag="VFL49_10625"
     CDS             complement(2813..4057)
                     /locus_tag="VFL49_10625"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7912477.1"
                     /translation="MRQRDALAARCGRPIDVVSVCARSRSKDRGVDLKKMKWFAEPAF
                     LARDPGIDVFVELIGGAGEPARGAIEAALASGKSVVTANKALLARHGVKLAGLAERNH
                     SALNFEAAVGGAIPIVKTLREGMAGNSFERIYGILNGTCNYILTRMEQDKMSFAECLK
                     DAQRLGYAEADPSFDIEGHDTAQKLAILASLAFGTRADQSAIYVEGISSITPADLSAA
                     DELGYRVKLLGVAVRTTKGIEQRVHPTMVRKDSAIAQVMGVTNAVTIDAADINPLTLV
                     GPGAGGPATASAVLSDIGDIARGVRTAPFGRPTARLTTVRKAPMQRHEGGYYIRLVAR
                     DKPGTAATIATRLAEQDISLESIVQRHDPAHLGRDTKAGSVPVILITYATTEDAVRKA
                     LAAVGRDRVISGRPQVIRIEKN"
BASE COUNT          807 a         1269 c         1190 g          805 t
ORIGIN      
        1 caagatgacc aagaccggac aggtcgtctg ccctgtttgg gtcaacgatg gcaaccacat
       61 cgggaagcct ctcgtcggcc tgatgatggt cgatgataat ggtgccaaga ccgaagtttt
      121 tcgcttcggc cagaggttcg agactcgtca cgccacagtc gaccgtaatc agaagccttg
      181 caccgcgctg ggcgaacgaa cggatcgcat ccacgttcgg cccatagcct tcaaaaacgc
      241 gatccgggat atggacgatg gggtccaggc cgcaatggcg cagatagcgg cagagcaaag
      301 ccgcagcggt cgcaccatcg acgtcatagt ctccgaaaat ggcgacactt tcgccgccga
      361 tcaccgcgtc ggcaattcgg acagatgcgg cctgcatatc cgtaagggta tacggatcgg
      421 gcagaagacg cttgatggtg ggatcgagaa actcttcgac actgtcgact tcgacgttgc
      481 gtccggccaa aacacgggca agcaattcgt cgagaccgtg acgctgcgcg atggccaaag
      541 cgcgcgcgga cccgcgtgcg tcgagccggt cgcgccaggc tcgccccgtc agcgaacgtt
      601 cgacgcccag gaaatgacgg tcactttcgt ttaactgagc ggcaggcagg gccattgcgt
      661 gttggttggg ttacgggctt ggccagcccg caaagaatcg gttttcggaa gtcccatata
      721 gtggcgaagg ccgcccgacg gtaccagagg tcatccggcc agttcagcgt gaatacgcaa
      781 caattccggt atggtctttg ggtcgcgccg gccaaatccg cactcggtgg cgatcgaaaa
      841 cgccttcgca tgccgttcgg cggttgcgag ccgttgccgt gttccagtta caccgtcggt
      901 gaaatgaacc aggccgaggc tcagttcggt ttccggtttg aaccgcaggc gcgcgagcgg
      961 ctcgaaatag gcatcgtcat tgcggtttcg cggcaccggc atgtggatta gttgaacgct
     1021 ccgcttgata tccgcaaaaa gctcgtttgc gaggtcgacc atatcgccca tatcggtcgg
     1081 ctcaacaaca tgcctgtggt tggagtcgcc gtaacagaag tggaacagga gctcgatatc
     1141 acgcggcaca tggtcggcaa gatccgtgag aattgccgaa aatgatttca gcatctccgc
     1201 gcgtgtcttc ccatacgcgt tgggctcgcc ccgctgcagg cgggcgaaca cggccgaagc
     1261 gacgtcaaac tggattgcta tctggtcgtg cggcagcgcc cccgcgatct tgtcgatttc
     1321 ccgtttgagc gcctcgttat agaccgggtc aagcggccga tgcagatcct cctgcacaaa
     1381 taaccaaata acggaatgcg ccggcaccag atcgatctgc aagcggcaat ctttcggcac
     1441 tattccttcc cgcttgagag tcgaaaaatc cgcatacgac gccttggcaa tatcggcgta
     1501 gaaaagattg ccaaagcgca cgtcacgcat gctccgtccc gaacgcaacc gatagcggat
     1561 acgcgcggtg cccgttgcgt gcacgcgaaa aagctcgtcc gacttctcca gtgccggatt
     1621 ctcggcgaag gccggctcca gccacgtaat ccagtcggag cgttcccccg tctcaccgtc
     1681 agggatgcgc ctgagatacg gaccaagcgc cttgcttact gtagcgaaga cctcacgcgc
     1741 atcacggagc ggcacgctgc cgacgaggtg aacgtggcgc atgggactca gtcaagatga
     1801 aatttgtcga actggcggtg ttcagccagg acccgccgca ctgtgccggt caccgaccgc
     1861 atcacgacag tttcggtttc gatcacatct ttgccgaact tgacaccgct gagcatcgcg
     1921 ccggacgtga ccccggtcgc cgcgaacagg cagtcacccc gcaccatgtc ttcgatctga
     1981 tattttttcc tcgggtccct gatgcccatc ttcaaggtcc gctcgcgcag ctcttcagtg
     2041 tcgataatca gccgcgtctg catctgcccg ccgatgcagc gcaacgctga cgccgcgagc
     2101 acgccctcgg gcgcaccacc gatgccgaga tagatatcaa taccggtctt cgcgtctgcg
     2161 caatggatca cgccggcgac gtcgccgtcg ctgatcaggc tgaccgcggc gccggtcttg
     2221 cgcacggccg caatgatatc ggcgtgacgt gggcggtcga gcaggatcgc ggtgatctcc
     2281 gaggccttca cgcccttggc ctttgcaagc gaaagaatgt tttctgccgg tggcgcatcg
     2341 aggtcgatcg tgcccttggg atagccggga ccgatcgcga tcttgtccat atagcagtcg
     2401 ggcgcatgca gcagcgtccc ggcatccgcc attgccaggg tcgcgatggc gccgggcata
     2461 ttcttggcgc aaagcgtcgt tccctcaagt ggatcgaccg caatgtcgac tttcggtccg
     2521 gttttgttgc cgactttttc tccaatgaac aacattggag cctcatccat ctcaccctca
     2581 ccgatgacga cggtgccatc aatcggcagc ttgttcagtt cgcgccgcat ggcatcgaca
     2641 gccgcctggt cggaggcctt gtccttgccg tgcccgcgca agcgggccga agagacggcc
     2701 gcgcgctcgg tcacacgcac gatctcaagg ctgaggatgc gttccagcag caattccggc
     2761 tggacggtaa tacttggcat ttgacgctcc ctcacagaca ctcaggccgg cttcaattct
     2821 tttcaatccg aatgacctgc ggacggccgg agatcaccct atcgcggccc actgccgcaa
     2881 gtgctttgcg caccgcatcc tcggtcgttg catatgtaat aaggataacg gggacggaac
     2941 ccgccttggt gtcgcgcccc aggtgagccg gatcgtgacg ttgcacaatc gattccaacg
     3001 aaatgtcctg ttccgcaagt cgtgtggcga tagtagccgc ggttcccggc ttgtcacgcg
     3061 caacaagccg aatgtaatag ccgccttcgt gccgctgcat tggcgctttc cgcactgtgg
     3121 taaggcgcgc cgtaggccgc ccgaaaggcg cggtgcgtac gccccgggca atatcgccga
     3181 tatcggacag aaccgccgaa gctgtggctg gtccgcccgc tccgggtcca accagggtga
     3241 gtggattgat gtcggcggcg tcgattgtga cagcattggt cacgcccatc acctgtgcaa
     3301 tcgccgaatc tttgcgcacc atggtcggat gcacccgctg ctcgatgccc tttgtcgtcc
     3361 ggactgcaac gcctaacagt ttcacccggt aaccaagctc gtccgccgct gaaagatcgg
     3421 ccggcgttat cgatgaaatc ccttccacat agatcgcact ctggtcggct ctggtgccga
     3481 acgcgaggct cgcgagaatg gcgagcttct gcgcagtatc gtggccctca atatcgaaac
     3541 tcggatctgc ctcggcataa ccgagacgct gggcatcctt caggcattcc gcaaaggaca
     3601 ttttgtcctg ctccatccgc gtcaggatgt agttgcaggt gccgttcagg atgccataga
     3661 tgcgctcaaa cgaattgccg gccattccct cgcgcagcgt cttgacaata ggaattgcgc
     3721 cgccgaccgc ggcctcaaaa ttgagcgcgc tgtgattgcg ctcggcaagc cccgcaagct
     3781 tgacgccgtg tcgcgccagc agcgccttgt tggccgtgac aaccgatttt cccgaagcca
     3841 gggccgcttc aattgcgcct ctggccggct cgccggcgcc gccgatcagc tcgacgaaaa
     3901 cgtcgatgcc cggatcgcgc gcgaggaatg caggctcggc aaaccatttc attttcttaa
     3961 gatcgacgcc ccgatctttg ctgcgcgacc gcgcgcagac ggaaacgacg tcgatcggac
     4021 gtccgcagcg cgccgccaat gcatcgcgtt ggcgcacgac ttgcgcaagc a
//
LOCUS       DASSRL010000522         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1518897, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000522 DASSRL010000000
VERSION     DASSRL010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518897"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1783)
                     /locus_tag="VFL49_10630"
     CDS             complement(65..1783)
                     /locus_tag="VFL49_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HET7912478.1"
                     /translation="MAKGGILRSLLLASCLGVFGAGANAAPFTINDALRMAVQTNPSV
                     GEAAANKRATEAELRQVQSTLLPQVRLEASAGPERLNNPATKPLNTIVPTLNSGEWVA
                     GAKGSIFIRQLLFDGFTSLHEIWRNAARVDAAAARTHERSELIALDASEAYIDVVRYT
                     RLVALAQENVAAHKRIFGNVEQRFRGGRAGQGDLEQTRERVDAAEVALTEFRRSLEDA
                     RSKFRKVIGVEPYNLRGPGRLPGLPPSKDRVLAVTLTDNPTIKAAQADTDAAREAFHS
                     AAGAFMPNVALEARGTEGHDTDGFPQHFTESSLKVVATWDIFRGGQDTWKRVETAERY
                     NQTTMAHARLQRDANESVDKAWAARVITADRIAALQLQTEAARKVIEAYSKEYDLGQR
                     SLIDLLNAENTSFNAQVSLISARSVAIFADYQLLAAMGRLLDYVRAPHPVDAEPLVPP
                     GNYGLIPVKLPPILINIPQPGPEPLNVAGPRTQPVISGYVPPTPGRPIPSPVPGAGAR
                     ASADDGAAAPKVGFADRWLDPTATRHAGGIVAAETSLGYSAPGIFTPGQLMKMPQWPL
                     NEAAKK"
     gene            complement(2051..2230)
                     /locus_tag="VFL49_10635"
     CDS             complement(2051..2230)
                     /locus_tag="VFL49_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912479.1"
                     /translation="MTNALIELQREFIEHLNNAGRDVAIAKRDFEDLMQGLAVWVQQR
                     QRVRASEPVTRSDVA"
BASE COUNT          514 a          842 c          886 g          574 t
ORIGIN      
        1 ggtcgcagga ccgccctttg gttaatcccg aagaacagcc cgaattaccg ggctatttcc
       61 ttgattactt cttggctgcc tcgttcaggg gccattgcgg cattttcatc agctgaccgg
      121 gcgtgaagat gcctggcgcc gaatagccga gggaggtttc ggccgcgacg attccacccg
      181 catgacgggt ggcagtcgga tcaagccagc ggtcggcgaa gccgaccttc ggcgccgcgg
      241 cgccgtcatc cgcgcttgcc cgcgcgcctg ctccggggac gggtgacggg atcgggcggc
      301 ccggcgtcgg cggcacatag ccggagatca ccggctgggt gcgcggcccg gcgacgttaa
      361 gcggctcggg gcccggctgc gggatgttga tcaggatcgg cggcagtttg accgggatca
      421 ggccgtaatt gccgggagga acgagcggct cggcatcgac cggatgcggc gcacggacgt
      481 agtcgagcag cctgcccatc gcggcgagca gctgatagtc agcgaaaatc gcgacactgc
      541 gcgcggagat caacgacacc tgcgcattga aggaggtgtt ctcggcattg agcaggtcga
      601 tcagcgaacg ctgaccgaga tcgtattctt tcgagtaggc ttcgatgacc ttgcgcgcgg
      661 cctcggtctg cagctgcagc gcagcgatgc ggtcggcggt gatcacgcgc gccgcccagg
      721 ccttgtcgac ggattcgttt gcgtcgcgct gcaggcgggc gtgcgccatc gtggtctggt
      781 tatagcgctc ggccgtctcg acgcgtttcc aggtgtcctg gccgccgcgg aagatgtccc
      841 aggtcgccac gaccttgagg ctgctttcgg tgaagtgctg cgggaagcca tcggtgtcat
      901 ggccctcggt gccgcgcgct tcgagcgcga cattcggcat gaaggcgccg gccgcggagt
      961 ggaacgcttc gcgcgcggcg tcggtgtcgg cctgcgccgc cttgatggtc ggattgtcgg
     1021 tgagggtgac cgcaagcact cggtctttgg aaggtggcag gccggggagg cgtcccggtc
     1081 cgcgcagatt gtacggctct acgccgatca ccttgcggaa ttttgagcgc gcatcctcga
     1141 ggctgcggcg aaattcggtg agcgcgactt cggcggcgtc cacgcgttcg cgcgtctgct
     1201 cgaggtcgcc ctggccggcg cggccgccgc ggaaacgttg ctcgacgttg ccgaaaatcc
     1261 gcttgtgggc ggcgacgttt tcctgcgcga gcgcaacaag gcgcgtgtag cggacgacgt
     1321 caatgtatgc ctcagatgcg tcgagcgcga tcagctccga gcgctcgtgc gtgcgcgcag
     1381 cggctgcatc gacgcgcgcg gcgttgcgcc agatctcgtg cagcgatgta aatccatcga
     1441 acagtaattg acggatgaag atggagccct ttgcgccggc gacccactcg ccgctgttca
     1501 gcgtcggaac gatcgtgttc agcggcttgg tcgccggatt gttcagccgc tccgggccgg
     1561 cggatgcctc aagccggacc tgcggcagca gcgtgctctg cacctgccgg agttccgcct
     1621 cggtcgcccg cttgttggcc gccgcttcgc caacactggg attggtctgc accgccatgc
     1681 gcagcgcgtc gttgattgtg aatggcgctg cgttggcacc ggcaccgaaa acgccgagac
     1741 agcttgcaag caacaacgac cggaggattc cacccttagc catcccagac cccacttacg
     1801 acttcaattt cagaagaaat cataacattt cagaggttaa gcgctatgga atcagaggga
     1861 tactcaccag acggcggttc aaagccaagc ttatgtgaca caaaagtcac tttcccgatt
     1921 cgggacaacg cagaaatccg aaaagtttca aacgattagt gagggtaggt cactgccgca
     1981 aatttcgcgc cagcgtacaa aattaccaga atgagcgagg cgctttgcgg cgccggcctt
     2041 cataagcgaa ctaagccacg tcggatcgtg taacgggctc actcgccctt acgcgctgcc
     2101 tttgttgtac ccaaactgcg agaccctgca tcaaatcctc aaagtctcgc ttggcaatgg
     2161 caacgtcgcg gcccgcattg ttcagatgct cgatgaactc gcgttgcaac tcgatgagcg
     2221 cattcgtcat gcagaccatc cgctggatgg tcgctaattc gtgtcgacta atgtgcataa
     2281 ttggtaggcc cccagtcccc ggccgcactt taaatgtgcg caggcaaact gctgttactg
     2341 ctttgcaaca gtggaaaaaa atccgaatct gacgatttta caatgtgaaa tgtcgcagag
     2401 cacacaatcg acggggcaag gctgcccgtg gcctcatggt ccgccaatca gaacctgacg
     2461 gccggacggc tgaaaatacg gaaagcgacc ggaagattac ggcggcaacg acgaaatatt
     2521 ttctacgtta cttgcatgct cggttcgaat cgccgcctcg ccacggattc ccgacgttgc
     2581 aatcggcgct gaaatcgtcg gcttttcttg gcgctatacc ttctgcctca tcgcgttggc
     2641 gtgcttttgc gcgagccttc gcctcttcat cactcatctg atagcgcggc cgcaggtcgt
     2701 ctgttttgcg ccaatcgttt ctctgctctc gccagttatt gtagttcgag cgccagtcgt
     2761 ccctgtggat gcgccaatca tttgcgtcgc gcccgcgggg gttattggtg ttgaca
//
LOCUS       DASSRL010000523         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1448858, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000523 DASSRL010000000
VERSION     DASSRL010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448858"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VFL49_10640"
     CDS             complement(<1..1152)
                     /locus_tag="VFL49_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7912480.1"
                     /translation="MSEKLPVISVAEQLAASIAALRADRLPRALREKCIELLIDVAGL
                     CVTARNEDYIKSSLAGCDDDGPCTAIGHKRPFSAASAAFINGTAAHGEDFDDTFEGGP
                     VHAGAVIVPAVLAACERHRPDGPAALLGIAVGTEVMCRLSTVAPKAVHKAGFHPTAVF
                     GVMGAAAGVGAALGLNAKQIVDGLGIAASFAGGIIEYLAEGAWTKRLHPGWAAQSGIR
                     AALLARGGFFGPRTVFEGVHGLFHGFAHTTQGDYEAVTGGFGEQWVTETLAFKPYPCG
                     TMAHPYIDCSRRLAARGIKPGDIKEIICEVAEGTVHRLWEPLADKQHPPNGYAAKFAT
                     PFILATGFVRGSVGLDAFTDQAARDKDVLAIASKVRYVIDHDNPYPNSFT"
     gene            complement(1158..2876)
                     /locus_tag="VFL49_10645"
     CDS             complement(1158..2876)
                     /locus_tag="VFL49_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7912481.1"
                     /translation="MTIALPARAERRSLGIDVSRPVLYVFAGILCVLIVLPLSWLLMY
                     SVTDGKGGLTLANFYRLFTESSFREPLVTTFILATSSSFICCAVAAPMGWLVARTDLP
                     FSGVIRALVTASFVTPPFLGAIAWELLAAPNSGLLNKLARHWFGMATDQHLFNIYSLP
                     GVIFVISCYTFPYVFVLVANSLDRTPGELEDASSILGGGRWYTARRVTIPLVLPALLA
                     GALVAFLQAMTLFGSPAILALPAGFHTMTTKIWSLFQFPPKPELAAAASLPLLVLTVI
                     LLRAEHMILGRRGYSIVGGKYGAPRLIPLHRWRWPAFLLCLIVLLNPVFLPYAALFNA
                     AFSPIATTLLTIHNFTLQNVHFVLFELSAASLALKNTVILGTATATIGTALALVIAYV
                     TARQAVTGWRVLGFLATAPIAIPGIVLGVGLFLSYTRPPFVLYGTLWILLIAFVTIAL
                     PSAYQQLQAAFRTIHPELEDASRILGATRLRSLWQITAPLLRTGVIATWCFIFVGVMR
                     ELSAAIILFTSQTKVISVLIYDLNESGDLGAISVIGLGMLLITFAVVITVNRIPGFGN
                     VRFRNN"
     gene            complement(2946..>4047)
                     /locus_tag="VFL49_10650"
     CDS             complement(2946..>4047)
                     /locus_tag="VFL49_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7912482.1"
                     /translation="ETTRTAHGGKLMIRSRISRRDVLIGSSALAVGAAFSTKVMSAAP
                     PASAITPQLIEAAKKEGKVVWYTSVDLPLAEKIGKSFEAKYPGIAVRVERTGAERVFQ
                     RIGQEYGSNIHAVDVANSSDAAHFIVWKNKGMLEAYLPEDVAKFYPVEHKDPDGMFAS
                     FRVWVCIIAYNTNLVKKEEAPKSFADLLDPKWKGKIVKAHPGYSGTIMTATYQMQRDL
                     GWGYFEKLAQQNIMQVQSSADPPKRLALGERAVMADGNEYNIFQIKEKGGPVEPVYAT
                     EGSPLIVGPTGMFKAAPNPNAAKLFCNYSFTPECQQLIIDVGGLRSMHPNTKEHAGRI
                     PFKQIKVMKDDPAAVEKMSDEIKQHYVKIFHV"
BASE COUNT          857 a         1227 c         1234 g          729 t
ORIGIN      
        1 agtgaagctg ttaggataag gattatcgtg gtcgatcacg taacgaacct tcgacgcaat
       61 cgcgagcacg tccttgtcgc gggccgcctg atcggtaaac gcatcgagcc cgacactacc
      121 gcgcacgaaa ccggtcgcga gaataaacgg cgtcgcgaat ttcgcagcat agccgtttgg
      181 cgggtgctgc ttgtcggcaa gcggctccca aaggcggtgc accgtcccct cggcgacctc
      241 gcagatgatc tccttgatgt cgcccggctt gatgccgcgc gccgctagcc gtctggaaca
      301 atcgatataa ggatgcgcca tggtcccgca gggatacggc ttgaatgcga gagtctcagt
      361 cacccattgt tcgccgaaac cgcccgtgac tgcttcgtag tcgccctggg ttgtgtgcgc
      421 gaaaccgtgg aaaagcccgt gcacaccttc gaacaccgtc cgcggtccaa aaaagccgcc
      481 gcgcgcgagc agcgccgcac ggataccgga ctgcgccgcc cagcccggat gcagccgctt
      541 ggtccaggcg ccctcggcga gatactcgat gatgccaccg gcaaagcttg cagcgatccc
      601 aagcccatcg acgatctgct tcgcgttcag cccgagtgcc gcgccaacac cggccgctgc
      661 gcccattacg ccgaatacgg cggtcgggtg gaagccggcc ttgtgcaccg ccttcggcgc
      721 gaccgtgctc agccggcaca tcacctctgt tccaacagcg ataccaagca gagcggcagg
      781 cccgtcgggc cggtgccgtt cgcaggccgc aagcaccgcc ggcacgatca cggcacctgc
      841 gtggaccgga ccgccttcga aggtatcgtc gaagtcctcg ccatgcgccg cggtgccatt
      901 gatgaaagct gcgctcgcag cgctgaaagg gcgcttatga ccgatcgccg tgcatggacc
      961 gtcatcgtcg cagccggcaa gcgaactctt gatatagtcc tcattgcggg cggttacgca
     1021 cagaccggcg acatcgataa ggagctcaat gcatttttcg cgcagggcgc gaggcagccg
     1081 gtcggcgcgc agcgcggcaa tcgatgcggc cagttgctcg gcgaccgaga tgacgggaag
     1141 cttttctgac atggatctca gttgttgcgg aagcgtacgt tgccgaaacc gggaatacgg
     1201 ttgacggtga tcaccacggc aaacgtaatc agcagcatgc caaggccgat cactgaaatc
     1261 gcgccgagat cgccgctctc gttgagatca tagatgagca cagagatgac cttagtttgc
     1321 gacgtgaaca ggataatcgc cgccgacagc tcgcgcatca cgcccacgaa gatgaagcac
     1381 catgttgcga tcacgcccgt gcgcagaagc ggcgcggtga tctgccacag cgagcgcaat
     1441 ctcgtcgcgc cgaggatacg gcttgcgtcc tcaagctcgg gatgaatggt gcgaaacgcc
     1501 gcctgcagct gctgataagc ggacggaagc gcgatcgtca cgaacgcaat cagcaggatc
     1561 cacagtgtgc cgtaaaggac gaaaggtggc cgggtgtagc tgaggaacag gcccacgccg
     1621 aggacaatgc cgggaatggc gattggcgcg gtggcaagga agccgagaac acgccagccg
     1681 gtcaccgcct gacgggcggt gacataggca atgaccagcg caagcgccgt cccgatggtc
     1741 gcggtcgcag tgccgaggat cacagtgttc ttgagcgcga gcgaagcggc cgacagttcg
     1801 aacagcacga aatggacgtt ctgcagcgtg aaattgtgaa tcgtcagcag cgtcgtcgca
     1861 attggcgaga aggcggcatt gaataatgcg gcgtagggca aaaacaccgg attgagcagc
     1921 acaatcaggc acagcaggaa tgcgggccat cgccaccggt gcagcggaat aagtcgcggg
     1981 gccccgtatt tcccgccgac aatcgaatag ccgcgccgtc cgaggatcat atgctcggcg
     2041 cgcagcagaa tgacggtcag caccagcaac ggcagcgagg ccgcagcagc aagctcgggc
     2101 tttggcggga actggaacag gctccagatc ttcgttgtca tcgtatgaaa gcctgccggc
     2161 agcgccaaaa tcgccggcga gccgaacagc gtcatcgcct gcaggaaggc gaccagcgcc
     2221 cccgcaagca aagccggcag caccagcgga attgtcactc gccgcgccgt gtaccagcgg
     2281 ccgccgccca gaattgagga tgcgtcctcg agctcacccg gtgtccggtc gagcgaattt
     2341 gcgaccagca cgaacacgta agggaacgtg tagcaggaga tcacgaaaat cacgccgggc
     2401 agcgaataga tgttgaacag gtgctggtca gtggccatgc cgaaccagtg gcgcgcgagc
     2461 ttgttcaaca gcccgctgtt gggcgcagcc agaagctccc aggcgattgc gcccagaaac
     2521 ggcggcgtta cgaacgacgc cgtcaccagc gcgcgaatta caccggaaaa cggcaaatcg
     2581 gtgcgcgcga ccagccagcc catcggcgca gccacggcgc agcagataaa tgacgatgag
     2641 gtagcgagga tgaacgtcgt gaccagcggt tcacggaagg acgattccgt aaacagccgg
     2701 tagaaattgg cgagggtcag gccgcccttg ccatccgtca cgctgtacat cagcagccat
     2761 gacagcggca gcacgatcag cacgcaaagg ataccggcaa agacgtacag caccggccgc
     2821 gagacatcga tcccgaggct tcggcgttcg gcgcgcgcgg gcaaggcaat cgtcatgtca
     2881 acgaattcgg gttctgagcc ttgccgaact cgggacgagc cccgagttcg gctttgtccg
     2941 catcatcaga cgtggaagat tttcacgtag tgctgcttga tctcgtcgct cattttctcg
     3001 acggcggcag gatcgtcttt catcaccttg atctgcttga acgggattcg tcctgcgtgt
     3061 tcttttgtgt tcggatgcat ggaacgtaac ccgccgacat cgatgatcaa ttgctggcat
     3121 tcaggcgtga agctgtaatt gcagaacagc ttcgcggcat ttggattggg cgccgccttg
     3181 aacattccgg tcggcccgac gatcaacggc gagccttcgg tcgcgtagac cggttcgacc
     3241 ggtccgcctt tttccttgat ctggaagata ttgtactcgt tgccgtcggc catcacggcc
     3301 cgctcaccga gagcaagacg tttcggcgga tcggccgacg actgcacctg catgatgttc
     3361 tgctgcgcca gcttctcgaa gtagccccaa ccgagatcac gctgcatctg ataggtggcg
     3421 gtcatgatgg tgccgctgta tcccggatgc gccttgacga tctttccctt ccacttggga
     3481 tcgagcaggt cggcaaagct cttgggagcc tcttcctttt taacaaggtt ggtgttgtag
     3541 gcgatgatgc acacccagac acggaagctg gcaaacatgc cgtccggatc cttgtgctcc
     3601 accggataga acttggcgac atcctccggc aggtaggctt ccagcatccc cttgtttttc
     3661 cagacgatga aatgcgccgc gtctgatgaa ttggcaacgt cgacggcgtg aatgttgctg
     3721 ccgtactcct gaccgatacg ttgaaacacg cgctcggcac cggtgcgctc gacacgtacg
     3781 gcaatgccgg gatacttcgc ctcgaaagat ttgccgatct tctcggcaag cggcagatcg
     3841 accgatgtgt accagaccac cttgccttct ttcttggccg cctcgatgag ttgcggcgtg
     3901 atggcgctcg ccggcggcgc ggccgacatc actttcgtcg agaaggcggc gcccacggcg
     3961 agcgcactcg aaccgatcaa tacatcgcgg cgtgaaattc ttgaccgtat catcagcttt
     4021 cctccatgcg ctgttcttgt cgtttcg
//
LOCUS       DASSRL010000524         5624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_241575, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000524 DASSRL010000000
VERSION     DASSRL010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241575"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFL49_10655"
     CDS             <1..850
                     /locus_tag="VFL49_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7912483.1"
                     /translation="HLTGIFKLPLVTIALIFVLKAVASVISLGTGFRGGLFFASLLLG
                     ALGGQLFALGLGLVLPGLHIDPDSYAIIGMCALSVAVIGGPLTMTFIALETTGDLWLT
                     TAVLISAIIAMQVTREFFGYSFATWRFHLRGESIRGAADIGWIRDLTVGQMMRADVRT
                     TPADSPVAAFREAFPLGSTNQVIAVDEDGRYAGMVIVAEAHAAELGTTVPVRKILHHA
                     GHMLVPQMTAQEAIAAFDRYEAEALAVVDSLERRQVTGQLSEAHALRRYSDASERQRR
                     ALLGET"
     gene            complement(847..1029)
                     /locus_tag="VFL49_10660"
     CDS             complement(847..1029)
                     /locus_tag="VFL49_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912484.1"
                     /translation="MRKELMRAFDNTRAELERIEILVAGLAAFGTPVPNYEPTFRHLR
                     QLNLDAHELESDEPRT"
     gene            complement(1158..2312)
                     /gene="recF"
                     /locus_tag="VFL49_10665"
     CDS             complement(1158..2312)
                     /gene="recF"
                     /locus_tag="VFL49_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET7912485.1"
                     /translation="MTAAFIRRLTLSNFRGYHAAQMSLDRAGPVVLTGANGAGKTNLI
                     EAISLLAPGRGLRRATTEELAFSEGDGGWAISAEIEGMLGLATLGTGIDPPAGEESTP
                     TRKCRIDRESVGSATAFADHLRVVWLTPAMDPIFNGPASERRRFLDRFVLAVDAQHSS
                     RVAALERSLRSRNRLLEDSPGDSSWLDAVEHETAEVAVAVAAARVETVKRLSAALEAS
                     RDDAQEFPQAQVALQGWMEQLLPDHSAIEVEDRYRGQLKDNRARDTAAGRTLDGPHLS
                     DLVVTHAGKNIPATDASTGEQKALLIRLVLAHAGLIKQMTGFAPLLLLDEVVAHLDPS
                     RRAALYDALSRLGTQAWMTGADPAAFGDIAGRAQVFLVRGGAVEQSWGSV"
     gene            complement(2433..3551)
                     /gene="dnaN"
                     /locus_tag="VFL49_10670"
     CDS             complement(2433..3551)
                     /gene="dnaN"
                     /locus_tag="VFL49_10670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7912486.1"
                     /translation="MKVTVERAELLKSLGHVHRVVERRNTIPILANVLVKADRGKLSL
                     KATDLDLEVTDSIAAEVSPGGSTTVPAHMFYEIVRKLPEGSQIVLEGSGDRAVLSLRA
                     GRSRFTLQTLPESDFPDLAAGEMSHSFKLAAADLKRLIDKTQFAISTEETRYYLNGIY
                     LHVAGTAKSGTLRAVATDGHRLAQVELPLPQGADGMPGIIVPRKTVGEVQRLIETGEG
                     DVAIELSSAKIRFTIGNVVLTSKLIDGTFPDYGRVIPVNNDKSLVVDKKDFEAAVDRV
                     STVSSERGRAVKLSITGGRLVLSVTNPDSGSATEEIEVDYAADPLDIGFNSRYLLDIA
                     AQIEGEVAVLKLADPGSPTLIQDQDAKGALYVLMPMRV"
     gene            complement(3795..5216)
                     /gene="dnaA"
                     /locus_tag="VFL49_10675"
     CDS             complement(3795..5216)
                     /gene="dnaA"
                     /locus_tag="VFL49_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7912487.1"
                     /translation="MSNGELESWSRVKERLRTEVGDDVYSSWFARMDLEAIEDGAVRL
                     SVPTRFLKSWIQSHYAERVLACWQAQEDKVTRVELIVRSAVLKSAVLPKAKTDQAVGD
                     NGSRYINGNGRPLVAGDPSVHEALGGSPLDPRLTFDTFVVGRSNTLAHAAAKQVAGAR
                     RSDAVMFNPLYIHSGVGLGKTHLLQAVTWAGNAAGERKVLYLTAEKFMYGFVSALRTQ
                     TALAFKEALRGIGVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIASDRPPSDLE
                     SLDDRVRSRLAGGLVVEMGPLGEELRFEILKARVMAARNHHPAFDVPLPVLGYIAKTV
                     THNGRDLEGALNRLLAHNKLTGHAVTLEMAEREVRDLIRPQEPKRVKIEDIQRVVARQ
                     YNVSRSDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVRKI
                     EGLVGNDMALAEEIEILKRQLQE"
BASE COUNT         1013 a         1740 c         1815 g         1056 t
ORIGIN      
        1 gcatctgacc ggcatcttca aattgccgct tgtcaccatc gcgctgatct tcgtgctgaa
       61 ggccgtcgca tcggtgattt cgctcggcac cggcttccgc ggcgggctgt tcttcgcttc
      121 gctgttgctc ggggcgctcg gcgggcaatt attcgccctc ggcctcggct tggtactgcc
      181 gggtttgcat atcgatcccg actcctacgc gatcatcgga atgtgcgcgc tgtcggtggc
      241 ggtgatcggc ggtccgctga ccatgacctt cattgcgctg gagaccaccg gtgacctctg
      301 gctcacgacg gcggtgttga tttcggcgat cattgcgatg caggtgacgc gcgagttctt
      361 cggctattca ttcgccacct ggcgctttca tctccgcggc gagtcgatcc gtggcgctgc
      421 cgatatcggc tggatccgcg atctgacggt gggccagatg atgcgggccg acgtgcgtac
      481 cacgccggcg gattcacccg tggcggcgtt tcgcgaggcc tttccgttgg gctcgaccaa
      541 ccaggtcatc gcggtggacg aagacggccg ctatgcaggc atggtgattg tcgcggaagc
      601 ccatgcggcg gagctcggta ccacggttcc tgtccggaaa attctgcacc acgccggtca
      661 catgcttgtt ccgcaaatga cggcgcagga ggcgatcgcg gctttcgatc gctacgaagc
      721 ggaagctttg gcggtggtcg actcgctgga gcgccggcag gtcaccggcc aattgagcga
      781 ggcgcatgcc ttgcgccgct actcggatgc ttcggagcgc cagcggcgcg ccttgctcgg
      841 ggaaacctag gttctcggct catccgactc gagctcgtgc gcgtccagat tcaattggcg
      901 gagatgcctg aacgtcggct cgtaattggg aaccggcgtg ccgaacgcag caaggcccgc
      961 caccagaatc tcgatgcgct cgagctcggc acgcgtgttg tcaaaggcgc gcatcagctc
     1021 ctttctcacg cgatcgattg cgacatccgt tcccattgca gcccctgctt catcttacgg
     1081 ggtaaaatcc agcgccgggc gatggttcct attttccaag aattgatcgg atttcgatta
     1141 acaaaacgtg agcgaggtca cacggaaccc cagctctgct ccacggcgcc gccacgaacg
     1201 aggaagacct gcgcgcggcc ggcaatatcg ccgaaggcgg caggatcggc gccagtcatc
     1261 caggcctgcg tgccgagccg cgacagcgcg tcgtagaggg ccgcgcgccg cgacggatcg
     1321 agatgggcga ccacctcatc gagcaggagc agtggcgcga accctgtcat ctgcttgatc
     1381 agtcccgcgt gtgcgagcac caggcggatg agcagggcct tctgttcgcc ggtcgaagca
     1441 tcggtggcgg gaatattttt gccggcgtgc gtcacgacga gatcggataa atgcgggccg
     1501 tcgagcgtgc gcccggcagc ggtgtcgcgt gcgcggttgt ccttcagttg cccacgatag
     1561 cgatcctcga cctcgatcgc gctatggtcg ggtaacaact gctccatcca tccctgcagg
     1621 gcgacctgag cctgtggaaa ttcttgcgcg tcatcgcgcg aggcttcgag tgccgctgac
     1681 agacgcttga ccgtttcgac gcgcgcggcg gcgacggcga cggcaacctc cgccgtttca
     1741 tgttcgacgg cgtcgagcca gctggaatcg ccgggcgagt cctcgagcag gcggttgcgc
     1801 gagcgtaagc tccgctcgag cgcggcgacc cggctcgaat gttgcgcgtc aaccgcgagc
     1861 acgaaccggt caagaaatcg ccggcgctcg gacgccggcc cgttgaaaat cgggtccatg
     1921 gccggcgtca gccacacaac ccgcaagtga tcggcaaagg cggtggccga accaacgctt
     1981 tcgcggtcga tgcggcattt gcgcgtcggc gtcgattcct ctcccgccgg cgggtcgata
     2041 ccggtgccga gcgtcgcaag ccccagcatg ccttcgattt cggcggaaat tgcccagccg
     2101 ccgtcaccct cggaaaaggc caactcttcc gtcgtcgccc gccgcaagcc ccggcccgga
     2161 gcaagcaggg aaatcgcttc gatcaggttg gtcttgccgg caccgttggc gccggtgagc
     2221 acgacgggac cggcgcggtc gagcgacatt tgcgcagcgt gataaccgcg gaaattgctc
     2281 agcgtcagac ggcgaatgaa ggcagcggtc atatgaatcc gtcatgcccg gcttcatgcc
     2341 gggcatccac gtctttcgtt tgtccgcact agcaagacgt ggatggccgg gacaagcccg
     2401 gccatgacaa aagaaaccag ctgcgggctt attcaaactc gcatcggcat cagcacgtag
     2461 agcgcgcctt tggcgtcctg atcctggatc aacgtgggag atccggggtc ggcgagcttg
     2521 agcacggcca cttcgccttc gatctgcgcg gcgatgtcga gcaggtagcg cgagttgaag
     2581 ccgatatcga ggggatcggc ggcatagtcg acctcgatct cctcggtggc gctgccggaa
     2641 tcgggattgg taaccgacag caccagccgc ccgccggtga tcgacagctt gacggcccgc
     2701 ccccgctcgc tggaaaccgt cgagacgcgg tcaaccgcgg cctcgaaatc cttcttgtcg
     2761 acgacaaggc tcttgtcgtt gttgaccgga atcacgcgcc cgtaatcggg gaaggtgccg
     2821 tcgatgagct tcgacgtgag caccacgttg ccaatggtga aacggatttt ggcgctggac
     2881 agctcgatag cgacgtcgcc ttcaccggtc tcgatcaggc gctgtacctc gcccacagtc
     2941 ttgcgcggca caatgatgcc gggcatgccg tccgcgcctt gcggcagcgg caattcgacc
     3001 tgcgcaaggc ggtggccgtc ggtcgcgacg gcgcgcagcg tgccgctttt ggcggtgccc
     3061 gccacgtgca gatagatgcc gttgaggtag taacgcgttt cctcggtcga gatcgcgaac
     3121 tgggtcttgt cgatcagccg cttgaggtcg gccgccgcca gcttgaaaga gtggctcatc
     3181 tcgcccgcgg cgagatcggg aaaatcgctt tccggaagtg tttgcaaggt gaagcgggaa
     3241 cggccggctc gcagcgagag cacggcgcga tccccggagc cctcgagcac gatctgcgaa
     3301 ccttcaggca gcttgcgcac gatttcgtag aacatgtggg cgggaacggt ggtcgacccg
     3361 cccggagaga cctccgcggc aatcgagtcg gtgacctcga ggtcgaggtc cgtcgccttc
     3421 aggctgagct tgccgcggtc ggccttgacc agaacgttgg ccaggatggg aatggtattg
     3481 cgccgctcga ccacgcgatg cacgtggccc agcgacttca gaagttctgc acgctcgact
     3541 gtgaccttca ttgaaaaatc cgcttcggcg ctcttgaatc agcccctgaa ccaggcgcga
     3601 cgatcagccg gcgtttcgca gagtgcgagg ccggggggca aaggtgaaca aagagagcgt
     3661 attgtgcaag ggcgcgtttt tgggcccggg tcatttcccc aagggcgtcc acaggacgga
     3721 agcacacgct cctgccaaag acagtgacgc ccggcacggg ccgggcatca ctatgagagc
     3781 aaatgcggcg ctctttactc ctgcagctgc cgcttgagga tctcgatctc ttcggcaagc
     3841 gccatgtcgt tgccgacaag gccttcgatc ttgcgcacag catgcaggac agtggtgtga
     3901 tcgcggccgc caaagcgccg gccaatttcc ggcagcgaac gcaacgtcag ggtttttgcg
     3961 agatacatgg cgacctgcct tgggcggacc acattggccg tgcgccgtga ggacaacaga
     4021 tcagaccggc tgacattgta ctgccgcgca accacccgct ggatgtcctc gatcttgacc
     4081 cgcttgggct cctgcggacg gatgaggtcg cggacttcgc gctcggccat ctccagcgtc
     4141 acggcatgcc cggtgagctt gttatgcgcg agaagccggt tgagcgcgcc ctccaggtcg
     4201 cggccgttat gagtgacggt tttggcaatg tagccaagca ccggcaacgg aacgtcgaaa
     4261 gcgggatggt ggttacgtgc ggccatgacg cgggccttga ggatttcaaa gcgcagttcc
     4321 tcgccgagcg ggcccatttc gactaccaga ccgccggcga ggcgggaccg tacccgatca
     4381 tcgaggcttt ccaggtccga gggtggtcgg tcggaagcga tcaccacctg ccggcctgca
     4441 tcgatcagcg cgttgagggt gtggcagaat tcggcctggg tcgacttgcc ttgcaggaac
     4501 tgcagatcgt cgatcaccag gacgccgatg ccgcggagcg cttccttgaa ggcgagggcg
     4561 gtctgcgtac gcagcgccga gacgaagccg tacatgaatt tctcggccgt cagataaagc
     4621 accttgcgct cgcctgccgc gtttccagcc caggtgacag cttgcaggag gtgcgtcttg
     4681 ccgagaccga cgccggaatg aatgtagagc gggttgaaca tcacggcgtc gctgcgccgc
     4741 gcgccagcga cctgtttcgc cgccgcgtgc gcaagggtgt tggagcgtcc aacgacgaaa
     4801 gtgtcaaagg tcaggcgcgg gtcgagcggc gagccgccga gggcctcgtg gacgctgggg
     4861 tcgccggcga caagaggccg gccgttgccg tttatgtaac ggctgccatt gtcgcctacg
     4921 gcctgatccg tctttgcctt gggcagaacg gcggatttaa ggacagccga gcgcacgatc
     4981 agttcgacgc gggtgacctt gtcctcctgt gcctgccagc aggcaagaac ccgctcggcg
     5041 tagtgcgact ggatccagct cttgagaaat ctggtcggca cggagagccg gactgccccg
     5101 tcttcaatcg cctccaggtc catgcgggcg aaccagctcg aatagacgtc gtcacctact
     5161 tccgtccgca ggcgttcctt cacccgcgac cagctctcta gttccccgtt ggacattgtt
     5221 cttctcgatc cgattaaagg tgggacggac tcggttgccc ggctcagcgg gaggccgcta
     5281 acgcgccgga aacgggacgc aacagaactt gttgaatgat gtcgttgtca gatggctccc
     5341 cgaagggagc caactcgccg gcggagcaac catcgatcgc tgtcgatggg tgccgcgcct
     5401 cacgtggaag gcggtggagt cgcgccttga atgccgctct caaaacagaa ggccataccg
     5461 gacatatgat tacgctgatc aataaatgaa tattgccgtg acgggtcggc gtcttggact
     5521 acggcgacag aaaaccccgc atcggacgcc ggccatccga cgcattttgg caccggtcgt
     5581 atcactcccc ctgtcatgac atcacccctc gaatgaaaac aggc
//
LOCUS       DASSRL010000525         4087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_976374, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000525 DASSRL010000000
VERSION     DASSRL010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976374"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VFL49_10680"
     CDS             <1..872
                     /locus_tag="VFL49_10680"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912488.1"
                     /translation="HRERGIALLMVLWVLILLGLIGASFLREARVSTNLARNSVENAK
                     AEALAEAGIRRAMLGMLDPDPATAWRADDTAYQFAFGGGLVVVRIQDERGKIDLNRAP
                     APLLLGLFAAVGVDPAVAGRLVDAIYDFRDPDHDRRAGGAEDADYLAAGLEGGAKDAP
                     FDQKEELLQVIGMEREIFDAIAPYVTVYSGRSRVDIATAPGVVLRAIPDLTPEQLDRM
                     QADKSNNARPGAIWADVVTVRAEAHTRGGGSFIREAVIMRTGDLLSPFAILEWRQEWR
                     RAPEFGEATAAQQ"
     gene            1117..2808
                     /locus_tag="VFL49_10685"
     CDS             1117..2808
                     /locus_tag="VFL49_10685"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7912489.1"
                     /translation="MSRYKNVAFAWVPMLAVAVLGGSGPAAVAANVNAVSDDVAEPGL
                     TPLTTSPKRLQPEPTIGIDPANPNIIAAGAQDFRKTTELNEACDGNRWNGLYISYNGG
                     KTWEQSLVPGYFTDPVFPQGTEQDESEQFGLCLNTDPVLVFDGLGNFYYSHVSFNDIP
                     QGTTTPSTVGVLYVSTYHLGENGKYAHAKTVKVPSASGLSMAGEIHEVGPGNSNFDDK
                     QWMTVDRSLDSPHYGRVYVTWTKFGAQGGQSSVWLSHCGGDFPGEACADDDGWSDGIK
                     VNKPVAGGLVQESYPATAPDGTIYVGFLQFQGGFGSSLPHAGIWIAKSTDGGVTFTQT
                     RIAPIRQIPSPIPPQGSAANDGGNSFRTGTVPGVVITPNDESDVGYSVHVTWGEWIDD
                     QQADVRYARSTDGGTTWETPLRLNDVSTGHQFFPSIAASGQNVHVAWLDSRLDTDLTQ
                     PITELQVAYTTFADGGAASATDTLVADQPFDPNEVSRFPVFCQAFIGDYIDIDVVPDR
                     HRDKVAIIWSDNRNVGTPLEPDECATLRQDPALDANQARLNDGSLDQDAFVEIDP"
     gene            complement(3004..3390)
                     /locus_tag="VFL49_10690"
     CDS             complement(3004..3390)
                     /locus_tag="VFL49_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912490.1"
                     /translation="MTIAVRWLLLMLILAVTAPAASVPVRAGTNDIVGAAAVRADGSL
                     RVGGHWIRLYGVYIPSTGKQCQTFLSPVYCGNRAAAALNLRIQGFVFCRTISRNSDGS
                     VNAFCSVDRTFYSGGEDLGAYLIARG"
     gene            complement(3622..>4087)
                     /locus_tag="VFL49_10695"
     CDS             complement(3622..>4087)
                     /locus_tag="VFL49_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912491.1"
                     /translation="HRLLYYREAVSGVATMKTVNSAAMTFLAMIAAAPALAQEAETFV
                     GSICEIDTSPLNVPYKTPDGTRSAFTFNTQKDCTGVASQRNIKLVCVSPPLPDWKFGN
                     RSAKNFPCTINPVQCGLSPKPTDNANPPFVTTFDSQLKVQSGVATLTCFYKP"
BASE COUNT          816 a         1220 c         1283 g          768 t
ORIGIN      
        1 gccatagaga acgcggcatt gccctgctga tggtcttgtg ggtgctgatc ctgctcggct
       61 tgatcggggc gagctttctt cgcgaggcga gggtcagcac gaacctggcc cgcaactccg
      121 tagaaaatgc aaaagccgag gcgttggccg aagccggcat ccggcgggcg atgctgggaa
      181 tgctcgatcc agatcctgcg acggcctggc gcgccgatga caccgcctac cagttcgctt
      241 ttggcggcgg gctcgtcgtg gttcgcattc aggacgagcg cggcaagatc gacctcaatc
      301 gcgcaccggc accgctcctg ctcgggctgt tcgcggcggt gggggtcgat cccgcggtgg
      361 ctggaaggct ggtggatgca atctacgact ttcgtgaccc ggaccatgac cgccgagcgg
      421 gtggtgcgga agatgccgac tatctcgccg ccgggctgga gggcggcgca aaggatgcgc
      481 ccttcgacca gaaggaggag ctcttgcagg tcatcggcat ggagcgcgag atctttgatg
      541 cgattgcgcc ttacgtaacg gtgtactccg gccggagtcg cgtagacatc gcaaccgcgc
      601 ccggcgtcgt gctgcgcgcc attcccgatc tgacgccgga gcagctggac agaatgcaag
      661 cggacaaatc aaacaatgcc cggcctggcg cgatctgggc cgacgtggtc accgtacggg
      721 cggaagcgca cacgcgcggt ggcggctcct tcattcgcga agcggtgatc atgcgtaccg
      781 gcgatctttt aagtcccttc gccatacttg aatggcgcca ggaatggcgg cgagcgcctg
      841 aattcggaga ggcgacggct gcgcagcagt gaggcgtgaa attgcaaaag aacacgtgca
      901 ggtgtgcact gtgccccgtg tcctgatttg atggccgttc cagtgaaatt ggcttacagc
      961 cgaggtttgc atttcgtcac tttacctctt cttatgcgtt cggtaccttc gaaaccaggt
     1021 agataaactg ttccaatgcc gctatcgcag cgtgcgtggt gagttcccta tctgcgccgg
     1081 tgacggtggc ggccagccag atgggaggaa acgaacatgt cccgctacaa gaacgtggcg
     1141 ttcgcctggg tgccgatgct ggcggtggcg gtgcttggcg gctcgggccc ggcggcggtg
     1201 gccgcgaatg tcaatgcggt ttccgatgac gtggcggaac cagggcttac accgctcacc
     1261 actagtccaa aacgactgca gccggaaccg acaattggga tcgatcccgc gaacccaaac
     1321 atcattgccg caggtgcgca ggactttcgc aagacgacgg agctgaatga agcgtgcgat
     1381 ggcaatcgct ggaacgggct ttacatctcc tacaacggcg gcaagacctg ggagcagagc
     1441 ctagtccccg gttatttcac cgatccggtg tttccgcagg gaaccgaaca ggatgaatcg
     1501 gagcagttcg gcttgtgcct caataccgat cctgtactcg tcttcgatgg tctcggaaac
     1561 ttctactaca gccacgtctc cttcaacgac attcctcaag ggacgaccac gccctcgacc
     1621 gtcggcgtgt tgtatgtttc gacctatcac ctcggcgaaa atggcaaata tgcgcacgcc
     1681 aagaccgtaa aggtcccttc ggcgtcgggc ttgagcatgg ccggggagat ccatgaagtc
     1741 gggccgggca attcgaactt cgacgacaag caatggatga cggttgaccg ctcgctggat
     1801 tcgcctcact acggccgggt ctatgtgacc tggacgaaat tcggggcgca aggcgggcaa
     1861 agctccgtct ggctgtctca ttgtggcggg gatttccctg gcgaagcctg tgccgatgat
     1921 gacggctggt cggacgggat aaaagtgaac aagccagtcg ccggcggact cgtgcaggaa
     1981 tcatatccgg cgaccgcgcc ggacggcaca atctatgtgg gctttcttca gttccaaggc
     2041 ggcttcggtt cttcgctgcc tcatgcaggc atctggattg ccaaatcaac ggatggcggc
     2101 gtgaccttca cgcagacgcg gatcgcgccc atccgccaaa ttcccagccc gattccgccg
     2161 caaggcagcg ccgccaatga cggcgggaat tcgtttcgca ccggcacggt gccgggggtg
     2221 gtcattacgc ccaacgacga atcggatgtg ggctacagcg tgcacgtaac ctggggcgaa
     2281 tggatcgatg accagcaggc cgacgtccgt tacgcgcgtt cgaccgacgg cggaacgacg
     2341 tgggaaacgc cgctgaggct gaatgatgtg tcgaccggac atcaattctt cccctcgatt
     2401 gcggccagcg gccagaacgt acacgtggcc tggctggaca gccggctcga cacggacctc
     2461 acgcagccga tcaccgagct gcaggtcgcc tacaccacgt ttgccgacgg cggcgctgcg
     2521 tccgccaccg acacactggt tgccgaccag ccgttcgatc caaacgaggt cagccgtttc
     2581 cctgtcttct gccaagcctt catcggcgac tatatcgata ttgacgtagt gccggatcgc
     2641 catcgcgaca aggttgcgat catctggagc gacaatcgca atgtcggcac tccgcttgaa
     2701 ccggatgagt gcgcgacctt gcgccaggat cccgctctgg atgccaatca ggcgcggctg
     2761 aatgacgggt cgcttgatca ggatgccttc gtcgagatcg acccttgagg aaccgcagca
     2821 ggccgggcgg cgacggccgg cctgtccctt cacccggtta accgaatagt ccataagagc
     2881 tgcgagagcg gggctatcgt cggatgatcg agtctgcagg aaagccccag atgccgagct
     2941 gccgcgtctg cgcgatgcgc tccatggcga cgtattcgaa cggcgcatat ggccccgcca
     3001 gcgctagccg cgggcgatca agtacgcacc cagatcctcg ccgccggaat aaaacgtgcg
     3061 gtcgacggag cagaaggcat tcacgctgcc gtcgctgttg cggctgatgg tgcggcagaa
     3121 cacgaacccc tgaatacgca agttgagcgc ggcagccgcg cgattgccgc aataaacggg
     3181 cgacaagaag gtctggcact gcttgccggt cgatggaatg tagacgccat agagccggat
     3241 ccaatgcccg ccgacacgca gcgatccatc ggctcgaacg gcggcggccc caacgatatc
     3301 attcgttccg gcacgcaccg gaacagatgc cgccggcgcc gtcaccgcca ggatcagcat
     3361 gagcagcagc cagcggacag caattgtcat gggccgagcc tgacacacgt gggaatatgt
     3421 tatacctcac aaccgtgtgc gcaacggatg gacaagcgat cacggcctca tagccagcgc
     3481 tgccgcgtcg agcgcggcca tggtcaagca acaaccggtc gcccataatt atggcgcgct
     3541 tgatcggccc ttcggcgaac ccgcggagca cgcgccgcct ggccgccgcg cctgcgggcg
     3601 ctcactcata cagcggaccg ctcagggctt gtagaagcag gtcagcgtcg caacgccgct
     3661 ttggaccttc aattgagaat cgaatgtcgt cacgaacgga ggattcgcgt tatcggtagg
     3721 cttcggtgac aggccgcatt ggactggatt gatcgtgcat ggaaagttct tggccgatct
     3781 gttcccgaac ttccagtccg gcaagggggg gctaacgcat acaagtttga tattgcgctg
     3841 agaggccacg cccgtgcagt ccttctgcgt gttgaacgta aaggccgatc gggtgccatc
     3901 gggcgtctta tagggaacgt tcaatggcga cgtatcgatc tcacatatgc ttccaacaaa
     3961 cgtttccgcc tcctgggcta aggcaggcgc tgcggcgatc atggccagga acgtcattgc
     4021 ggctgagttg acggtcttca tcgtcgctac tccactcacg gcttctcgat agtaaagcag
     4081 tcggtgt
//
LOCUS       DASSRL010000526         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1676002, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000526 DASSRL010000000
VERSION     DASSRL010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676002"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFL49_10700"
     CDS             <1..1161
                     /locus_tag="VFL49_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7912492.1"
                     /translation="LSMSQLPSLDAEGPAVQLAPVAVEDLLLRPSVKIDYRRLENFVS
                     AKAIVVTGGGGSIGSEICDRIVTFGASRLLVIEHSEPALHAVLETLRAKQSSTEIVGR
                     IADVRDRHRIAGLVAQFRPDIVFHAAALKHVPMLEQDWDEGIKTNVFGSINVADAAAA
                     AGAAAMVMISTDKAIEPVSVLGATKRLAEMYCQALDGELSRRSQNPMRLISVRFGNVL
                     ASNGSVVPKFKAQIEAGGPVTVTHPDMVRYFMTIREACDLVVTAASHASEQANGDASV
                     YVLNMGQPVKIVELAERIIRLSGLEPERDIKITFTGIRPGERLHEILFARGEETSEIG
                     IPGIVAAKPVSPSLETIRAWVATLDQGVARGERATIYNVLRDAVPEFRGEAA"
     gene            1173..1760
                     /locus_tag="VFL49_10705"
     CDS             1173..1760
                     /locus_tag="VFL49_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7912493.1"
                     /translation="MDLTAFFKAEFAEHNEVARRTEAALSDAFVKLVLACANSVDAGG
                     KLMFFGNGGSAADAQHLATELTIRYKKNRAAIAAIALTTDSSALTAAGNDLGFEQIFA
                     RQIEALGRPGDVAIAISTSGKSANVIAGLKQAKAMGLVTAAFGGNDGGALPAHADHVL
                     VVPSATTARIQEMHILLGQMLCGALEIELDLATGP"
     gene            1870..>2378
                     /locus_tag="VFL49_10710"
     CDS             1870..>2378
                     /locus_tag="VFL49_10710"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7912494.1"
                     /translation="MKATILPGPGAKPRAQRTREGDGHSIQSVDRALFLLETIAEAGG
                     EATLTELANRTGLNISTCHHLLATLIQRGFAAKVPGRRLYALGPRIIYLGHACLQVDL
                     PRRAQPYLETVNQATGETVHLAALQGDSVVTLSVREARHAVRVDTGRIGKVEAPHATS
                     VGKAIMAWLP"
BASE COUNT          465 a          717 c          743 g          453 t
ORIGIN      
        1 ctgagcatga gtcagcttcc gtcgctcgat gccgaagggc cggctgtcca gcttgcgccg
       61 gtcgctgtcg aagaccttct gctgcggccg agcgtgaaga tcgactaccg gcggcttgag
      121 aattttgtga gcgcaaaggc catcgtcgtg acgggcggcg gcggctcgat cggttccgaa
      181 atctgcgacc gcattgtcac tttcggagcc agccgcctgc tggtgatcga gcattccgag
      241 cctgccctgc acgcggtgct ggagaccctg agagccaagc aaagctccac tgaaattgtc
      301 gggcggattg ccgatgtgcg cgatcgccac aggatcgccg gcctcgtcgc gcaattcagg
      361 ccagacatcg tttttcatgc ggcggcgctc aagcacgtgc cgatgctcga gcaggattgg
      421 gacgaaggca tcaagacgaa tgtattcggc tcgatcaacg tggccgatgc agccgcagcc
      481 gccggcgccg cggcgatggt gatgatatca accgacaagg cgatcgaacc ggtttccgtg
      541 ctgggggcta cgaagcggct ggcggagatg tattgccagg cgctcgacgg tgagttgtcc
      601 cggcgcagcc agaatccgat gcgactcatc tcggtgcgtt tcggcaatgt gctcgcctcc
      661 aacggctccg tcgttcccaa attcaaggcg cagatcgagg cgggcggtcc ggtaacggtg
      721 acacacccgg acatggtccg ttacttcatg acaattcgtg aggcctgcga tctcgtcgta
      781 acggccgcaa gtcacgcatc ggaacaagcg aacggagatg catcggtcta cgtgctcaac
      841 atggggcagc cggtgaagat cgtcgaattg gccgagcgca ttattcggct cagcggtctt
      901 gagccggaac gtgacatcaa gattactttc accggtatcc gtcccggcga gcggctgcac
      961 gagattcttt ttgcacgcgg tgaggagaca tccgagatcg gcattcccgg tatcgtcgcc
     1021 gccaagcctg tttcgccctc gcttgagacg atccgcgcgt gggtcgcgac cctagaccag
     1081 ggcgtggcgc ggggtgaacg cgcaaccatc tacaatgtct tgcgcgacgc ggtccctgaa
     1141 tttcgcggtg aggcggcgtg agaggagcgg ccgtggacct gacagcgttc ttcaaagcgg
     1201 agttcgcgga gcacaacgaa gttgcgcgcc gcaccgaagc agcattgtcc gacgcgttcg
     1261 taaagctggt actggcttgc gcaaactctg tcgacgccgg tggtaagctg atgtttttcg
     1321 gcaatggtgg cagcgcagct gacgcgcagc atctcgcgac cgagctgacc atccgctaca
     1381 aaaagaatcg cgccgcgatc gcggcgatag cattgacgac cgacagttcg gcgctcaccg
     1441 cggccggcaa cgatctgggg tttgaacaaa tatttgcccg ccagatcgag gcgctgggcc
     1501 ggcctggcga tgtcgccata gctatttcaa cctcgggcaa aagcgcgaat gtcatcgccg
     1561 gcctgaaaca ggcgaaagcc atgggcctcg tcacggcggc gtttggtgga aatgacggcg
     1621 gtgccttacc tgcgcatgcc gatcacgtgc tcgtcgtccc gtcggcgacg accgcccgca
     1681 tccaggaaat gcatattttg cttggccaaa tgctctgcgg ggcccttgaa attgaactgg
     1741 acctggcgac aggtccatag atctttgacc gcagaatttt aatattacga aaattacctt
     1801 ccttatgctg aaaagcgctt gactccccgt ggtcggcgat tttcccttgc ggcaagggag
     1861 aaacgaccaa tgaaggcaac gatcctgcct ggacctggtg ccaagccgcg tgctcagcgc
     1921 acgcgggaag gtgacggcca cagcattcaa tctgtcgacc gcgcattgtt tttgctggaa
     1981 acaattgcgg aggccggcgg cgaggcgacg ctgacggagc tcgccaatcg gaccggcctc
     2041 aatatttcga catgccatca cctgctggcg acgctcatcc agcgcggttt cgcggcgaag
     2101 gtgccgggcc ggcgtcttta cgccctcgga ccacgcatca tctatctcgg ccacgcgtgc
     2161 ctgcaggtcg acctgcctcg ccgcgcgcag ccttatctcg aaaccgtcaa ccaggcgacc
     2221 ggcgagaccg tccatctcgc cgcactgcaa ggcgacagtg tcgtcacatt gtccgtgcgc
     2281 gaggcgcgtc acgccgtgcg cgtcgatacg ggcagaatcg gcaaggtcga ggcgccgcac
     2341 gccacatcag taggcaaggc aatcatggcc tggctgcc
//
LOCUS       DASSRL010000527         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_853935, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000527 DASSRL010000000
VERSION     DASSRL010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853935"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFL49_10715"
     CDS             <1..375
                     /locus_tag="VFL49_10715"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET7912495.1"
                     /translation="AKIAGNLMVACILEAMGEAAALARAYKMDPNTVLDAVVGSIFDV
                     PIYRIYSKMIANGPFEPPGFDLILGLKDAKLALDAGEAVNVPLPFASVLRDNYLDALA
                     HGDGTKDWSAVSRVAARRAGLE"
     gene            complement(380..1354)
                     /locus_tag="VFL49_10720"
     CDS             complement(380..1354)
                     /locus_tag="VFL49_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912496.1"
                     /translation="MFSLYFLPGRTSSAQTRRLGVLAVMACAAALIGYGLFSGSLEYL
                     NTLGLVFMYVVMTQAWNILGGYGGYFNFGMVTFFGVGAYTTALLFHFFGLSPFLTAPL
                     AGMASALLAFLIGIPALRLRGAYFAVVTMIVTFAIQILVLDLPFTQGAIGVFLVPLDL
                     SPRATEQLFYFVFLGLAAAMTALIYAIENSNFGWALVAIREDEDAAEIIGVRTTAIKW
                     IGNAIAGFATGLVGGVFAIRILYIEPTSTFSLDISLNVVLMAVIGGAGTWLGPLVGAP
                     LVLLVAEALRVTVTSEVNRVIFSLIVIVMALYMPRGLMGLMSRLRRKA"
     gene            complement(1364..2236)
                     /locus_tag="VFL49_10725"
     CDS             complement(1364..2236)
                     /locus_tag="VFL49_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912497.1"
                     /translation="MSIVLQIIADGVLLGLVYAVVGLGLSLLLGIMGIINVAHSAFIM
                     LGAFFTLGMLEMLHVDPIISFALAMPVFFVLGVIVFRIIIVRMERAEQNQGLVAMFGL
                     MVLIENVGTIFWTTNTRVVTVDYTNIRLHLGAVTVPTVRVIAGLLAIALIGAFWVFLN
                     RTLTGRAIRAMAQNQDAAVAAGINVTRLSLVMFGLGIAAAGAAGVVIAMVFPFAPQTQ
                     IEWLSWAFLIVILGGFGRVENTLAAGVLTGLIQTIATALMPFDYVYLLLYVALATVLV
                     LRREGLRGAVRRTI"
     gene            complement(2256..2984)
                     /locus_tag="VFL49_10730"
     CDS             complement(2256..2984)
                     /locus_tag="VFL49_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912498.1"
                     /translation="MTPPAELRVADVMAGYVPGHPILRGVNITAKPGRVTVVLGPNGA
                     GKSTMLRTVAGYLLPEAGRISLGETDITRLPAFQHVEHGIAFLPQGRSTFPDLTVSEN
                     IELGGWALRGDKTRLRQARDRVFARYPALLPLRDRPAGSLSGGQQRTVEIARLLMPDP
                     SVLLIDEPSVGLAPIIARQIYKELEAFRDEGRTILLVDQDVRAAIGIADYVYVLSSGK
                     NDIEGDRSAFEGDLGTMVRRWLGV"
BASE COUNT          689 a          958 c          920 g          474 t
ORIGIN      
        1 gcgaagatcg ccggcaacct gatggtggcc tgtatcctag aagctatggg agaggcggca
       61 gcgctggcgc gcgcctacaa gatggaccct aacaccgtgc tcgacgcggt cgtgggatcc
      121 atcttcgacg tgccgatcta ccgcatctat tcgaaaatga tcgccaacgg cccatttgaa
      181 cctccgggat tcgatcttat tttgggattg aaagacgcca agcttgcgct cgatgccggc
      241 gaggcggtga acgtgcccct gccctttgcc agcgtgctgc gcgacaatta cctggatgcg
      301 ctggcgcacg gcgatggcac caaggactgg tccgccgtct cgcgcgtcgc tgcacgccgc
      361 gccggtctcg aatagcaaat catgccttcc ggcggagccg ggacatcaac cccataagcc
      421 cgcgcggcat gtaaagcgcc atcacgatca cgataaggct gaagatcaca cgattgacct
      481 cggaggtgac ggtaacgcgg agcgcctccg ccaccaggag aacaagcgga gcgccgacca
      541 aagggccaag ccacgtgccg gcgccaccga tcaccgccat cagcacgaca ttgagcgaga
      601 tgtcgagcga gaacgtgctg gttggctcaa tgtaaaggat gcgaattgcg aagacgccgc
      661 caacgagccc ggtcgcgaag ccagcgatcg cattgccgat ccatttgatc gccgtggtgc
      721 gcaccccgat gatctccgca gcatcctcgt cctcacggat cgccactaac gcccagccaa
      781 aattggaatt ctcaatggcg tagatcagcg ccgtcatggc ggctgcgagc ccgaggaaca
      841 caaaatagaa cagctgctcg gtggcacgcg gcgaaagatc gagcggcaca aggaacacgc
      901 cgatcgctcc ttgggtaaac ggaagatcaa gcacgaggat ctggattgcg aaggtcacga
      961 tcatcgtgac caccgcgaaa taagcgccgc gcaaccggag agccgggatg ccgatcagaa
     1021 atgcaagcag cgccgacgcc atccctgcaa gcggcgcagt caggaatggc gacaacccga
     1081 agaaatggaa gagcaaggcg gttgtgtacg cacctacgcc gaagaacgta accatgccga
     1141 agttgaagta accgccgtac ccgccgagaa tattccacgc ctgcgtcatc accacgtaca
     1201 taaagacgag accgagcgtg ttgaggtatt cgaggctacc gctgaaaagt ccgtagccga
     1261 tgagagcggc cgcgcaagcc atgaccgcga ggacgcccag tcggcgtgtc tgggcggagg
     1321 acgtccggcc cggcaggaaa taaagagaaa acatccgcgc cccctagatt gtccgccgca
     1381 ctgcaccgcg cagcccttcg cggcgcagca cgagcacggt cgcgagcgcg acatagagca
     1441 gcaggtagac gtaatcgaac ggcatcaacg cggttgcgat cgtctggatc agcccggtca
     1501 gcacgccggc agcgagcgta ttctcaacgc gcccgaagcc accgagaata acaattagaa
     1561 aggcccaact cagccactcg atctgcgtct gtggcgcaaa gggaaacacc atcgcgatca
     1621 cgacaccggc ggcgcccgcg gcggcgatgc cgaggccgaa catcaccagc gatagccgcg
     1681 tgacgttgat gccagcagcg accgcagcgt cttggttctg cgccatggcg cggatcgcac
     1741 ggccggtcag tgtgcggttg aggaacaccc aaaaggcgcc gatgagggct atcgccagca
     1801 gcccggctat aacacgcacc gtgggtaccg tcacggctcc gagatggagc cggatattgg
     1861 tgtagtccac ggtcacgacg cgcgtattgg ttgtccagaa gatggtgccg acattttcga
     1921 ttagcaccat taatccgaac atcgcgacca gtccctggtt ctgttcggcg cgctccatgc
     1981 ggacaatgat gatgcgaaac acgatcacgc cgagcacgaa gaacacaggc attgcgaggg
     2041 cgaacgagat gatcggatcg acatggagca tttccagcat gccgagcgtg aagaacgcgc
     2101 cgagcataat gaaggcgctg tgagcgacat tgatgatgcc catgatgcca agcaacaggc
     2161 tcagaccaag gccgaccacc gcgtagacaa ggccgagcag aacgccgtcg gcaatgattt
     2221 gcagcacaat gctcatcgcg atgcaccgcg gtttgtcata cgccgagcca tcgccgcacc
     2281 atggtgccga gatcgccctc gaaggcggag cggtcaccct cgatgtcgtt cttgccggag
     2341 ctgagaacgt agacatagtc ggcgatgccg atcgccgcgc gcacgtcctg atcaaccagc
     2401 aggatcgtgc gcccttcgtc acggaacgct tccagctcct tgtagatctg ccgcgctatg
     2461 atgggcgcaa ggccaaccga cggctcgtcg atcaacagca cactggggtc aggcatcagc
     2521 agacgcgcga tctcaaccgt gcgctgctga ccgccgctga ggcttccggc cggccgatcg
     2581 cgcaacggca gcagcgccgg atagcgggca aagacgcggt ccctggcctg tcgcagccgc
     2641 gtcttgtcac cgcgcaatgc ccacccgccc agttcgatgt tctcgctgac ggtaaggtcc
     2701 ggaaacgtcg agcgtccttg cgggaggaag gcgatgccat gctcgacatg ctggaacgcc
     2761 ggcagtcgcg tgatgtcggt ttcgccaagc gaaatgcgcc ccgcttcagg caagagatag
     2821 cccgccactg tgcgcagcat cgtcgatttg cctgcaccgt tcggtccgag caccacagtc
     2881 acgcgccccg gcttcgccgt gatattgacg ccacgtagga tcggatgccc cgggacgtag
     2941 ccggccatca cgtcggcaac gcgcaactcg gcggggggcg tcattgcaga tcctcggccc
     3001 caccgagata gccctcgacc accttggaat cgcgcaccac c
//
LOCUS       DASSRL010000528         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_469264, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000528 DASSRL010000000
VERSION     DASSRL010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469264"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFL49_10735"
     CDS             complement(<1..182)
                     /locus_tag="VFL49_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912499.1"
                     /translation="MHGLYPNDASIYDSIGRLLIVAFFVIVWVRNLSSHHIEDHVKRL
                     TFFKAPLPRVTFWIGMA"
     gene            complement(208..1515)
                     /locus_tag="VFL49_10740"
     CDS             complement(208..1515)
                     /locus_tag="VFL49_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET7912500.1"
                     /translation="MTNTEIGLIGLGALFLLFVLRMPVGLTMLTVGLLGNAAIAGWSH
                     ALPSLSSETFAIATFYQLVVIPMFVLMGNLAGASGMGRDLYNAAYAWVGHIRGGLASA
                     TIIACACFASLCGSAAASAVTMGRVALPEMNRFKYDDALATGTVASGGTLGFIFPPSA
                     GMVIYAVLTEQALGRLFLAGVIPGIILTLLFLLVIRIITGRRQEAGPPGPQATRAERI
                     ATLKQASGIIIIIVMTIGGMYAGLFTPIEASGFGAGFTMILALLRRRLNWPVLRAVVL
                     QTMRTSATIFLILMGAYVFIPFMTLSQLPEILIKFLLSFDFSTTVILIIIITMYLALG
                     TFMEDFSMLILTLPVVMPVLKTLGVDLIWFGVIAVLIIQMGILSPPVGLAVFIVKGIV
                     PDVPMYTIFRGIWPFWYAMGALIILLLIFPQLALFLPNTMFGN"
     gene            complement(1512..2039)
                     /locus_tag="VFL49_10745"
     CDS             complement(1512..2039)
                     /locus_tag="VFL49_10745"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET7912501.1"
                     /translation="MMLGFIDKVTRIVALYCGGVVLAALMTVIIIDVTGRYLLNWPLF
                     GALDLSTVLLVLAVACAIGYGGRTGAHVTADMVSTLVGPRFEFYSGVAVKIAATAVVS
                     VWSWRLFVTGNVARRLGESTQLLNIPFEPIYKALSFGVGIYALVLAIETIVLIRTGKV
                     PLLIDESRAMRGENE"
     gene            complement(2112..>2676)
                     /locus_tag="VFL49_10750"
     CDS             complement(2112..>2676)
                     /locus_tag="VFL49_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912502.1"
                     /translation="VLTSREKPIRNIADLKGAKVLTPGAAFAPIVGAWGASPVPMDLN
                     EMFNALSTGVVDMVALPATSLTPPFRLAEIAKYTSRGLSGLFNPCGAIMNKESYAALT
                     PSQKAVFDKHTGKSLAMRAAKIFDDWEAESLGFAKGTNKIEVIELGPDVRKALFDAAK
                     PAVDKILADMKKDGVTNASEVYAALNK"
BASE COUNT          581 a          795 c          831 g          469 t
ORIGIN      
        1 gccatgccga tccagaaggt cacacgcggc aggggcgcct tgaagaacgt gaggcgcttg
       61 acgtgatcct cgatgtggtg gctcgacagg ttgcgcaccc agacgatcac gaagaacgcg
      121 acaatcagca gacgtccgat gctgtcgtag atcgaggcgt cgtttggata aagcccatgc
      181 atggtcgtct cccctgtgtt tctgctttca gttgccgaac atcgtgttcg gcaggaacag
      241 tgcaagctgc gggaaaatca gcagcaggat gatcagcgcg cccattgcgt accaaaaggg
      301 ccagatgccg cggaagatcg tgtacatcgg cacgtccggc acgatgcctt tgacgatgaa
      361 gacggcgagc ccgaccggcg gactcagaat tcccatctgg atgatcagca cggcgatcac
      421 gccgaaccag atcaggtcga cgccgagcgt cttgagcacg ggcatcacca cgggcagtgt
      481 caggatcagc atggagaaat cctccatgaa ggttccgagc gcgaggtaca tcgtgatgat
      541 gatgatcagg atgacggtcg tgctgaagtc gaagctcagc aagaatttga tcaagatctc
      601 gggtagctgc gacagcgtca tgaacgggat gaacacatag gcacccatca ggatcaggaa
      661 gatcgtggcg ctggtccgca tcgtctgcag cacgacggcg cgcaaaaccg gccagttcag
      721 gcgccggcgg agcaaagcaa gtatcatcgt aaagccggcg ccgaagccgg aggcctcgat
      781 tggcgtgaac aggccggcgt acatgccgcc gatcgtcatc acaattatga tgatgatgcc
      841 cgatgcctgc ttgagcgtgg cgattcgctc ggcgcgggtt gcctgcggtc cgggcgggcc
      901 ggcttcctga cgccttcccg tgatgatgcg gatgacgagg aggaagagaa gcgtaagaat
      961 gatgcctggg atgacgccgg cgaggaatag cctgccaagc gcctgctcgg tcaacacggc
     1021 gtagataacc atgccggcgc tgggcgggaa gataaagccc agcgtgccgc cggacgcgac
     1081 cgtcccggtc gccagcgcat cgtcgtactt gaagcggttc atctccggca gcgcgacgcg
     1141 gcccattgtc accgcagagg cagctgccga gccgcacagc gaggcaaaac atgcacaggc
     1201 aataattgtc gccgaggcca gtccgccgcg aatgtggccg acccatgcat aggctgcgtt
     1261 ataaagatcg cgccccatgc ccgacgcgcc ggcgaggttg cccatgagga cgaacatcgg
     1321 gataacgaca agctgataga acgtcgcgat cgcgaatgtt tcggagctga gcgacggcaa
     1381 cgcgtgcgac cagcccgcga ttgcggcatt gcccagcaag ccgaccgtca gcatcgtcaa
     1441 cccgaccggc atgcgcagga cgaaaaggag gaacagcgcg ccgagcccga tcaagccgat
     1501 ttcggtgttg gtcattcgtt ttcgccccgc atcgcgcggc tttcgtcgat caggagcggt
     1561 acttttccgg tacggatcag cacgatcgtt tcgattgcca gcaccaaggc gtaaatgccg
     1621 acaccgaacg agagcgcctt gtagatcggt tcgaacggaa tattgagaag ttgcgtgctc
     1681 tcgcccaatc gccgcgccac attgccggtg acgaacaaac gccaggacca cacgcttaca
     1741 accgcggtcg cggcgatctt gacggcaacg ccggaataaa actcgaaccg cggaccgacc
     1801 aaagtcgaaa ccatgtccgc tgtgacatgg gccccggtac gtccgccgta gccgatcgca
     1861 caagccaccg cgaggacgag caggactgta gaaaggtcga gcgcgccgaa cagcggccaa
     1921 ttgagcagat agcgacccgt gacgtcgatg atgatgactg tcatcagggc cgcgagcacg
     1981 acgccgccac agtaaagcgc aacaatgcgc gtgaccttgt cgatgaaccc caacatcatt
     2041 ggccccgagg cagccgaagc gccggctcgc ggacccggat accccgtctc cgcgtgcggg
     2101 tgtttcaccg cctatttatt gagtgcggca tagacctcgc tggcatttgt cacgccgtcc
     2161 ttcttcatgt cggcgaggat cttgtcgacg gcaggcttgg ccgcatcgaa tagagccttg
     2221 cggacatccg ggcccaactc gatcacttca atcttgttcg tgcctttagc gaatccgagg
     2281 gattccgctt cccagtcatc gaagattttc gcggcacgca tcgccagcga tttgccggtg
     2341 tgcttgtcga aaaccgcttt ctgcgacggg gtcaacgcgg cgtagctttc cttgttcatg
     2401 atcgctccgc agggattgaa cagcccagac aagccccggc tggtgtattt cgcgatctcg
     2461 gcgaggcgga acggtggtgt gagggaggtt gccggcagcg caaccatatc gaccacgccc
     2521 gtcgacagcg cgttgaacat ttcgttgagg tccatcggta ccggcgatgc gccccacgcg
     2581 ccgacgatag gcgcgaatgc cgcacccggc gtgaggacct tcgcaccctt gaggtcggcg
     2641 atgttgcgga tcggcttttc gcgcgaggtg agcacc
//
LOCUS       DASSRL010000529         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1326614, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000529 DASSRL010000000
VERSION     DASSRL010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326614"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFL49_10755"
     CDS             complement(<1..571)
                     /locus_tag="VFL49_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET7912503.1"
                     /translation="MALKGTNQEFGRPYNRRLVLETIRLHAPVARSAIAKRVGLTVQT
                     VSTIIRELDLQGFVRGARVRPRGRGLPPTFLAINPEGGFAIGIHVTPISLKAALINLA
                     GEVIGARERPLPQMRPDIAFREIGKLVAGLRKLRPDGRMLGIGLAMPGPFDVESMSFI
                     GPTTLEGWKGIPVQDRLVKLTGLPAFIEVD"
     gene            complement(741..817)
                     /locus_tag="VFL49_10760"
     tRNA            complement(741..817)
                     /locus_tag="VFL49_10760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(782..784),aa:Met,seq:cat)
     gene            complement(1290..2309)
                     /locus_tag="VFL49_10765"
     CDS             complement(1290..2309)
                     /locus_tag="VFL49_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912504.1"
                     /translation="MLAFPGGAIGAWLGDKPVVLIGLALMAIGEIAAATAPTWPILMG
                     ARIVSGTGGILLNVMIIKMVADWFTSKETATAMAIIGSSAPAGIALALATIPWIASSG
                     GRVLASISVVVYLVSAFVALAAAYKAPDEASAVAQRQSLWPERRVVVCLITAGLIYGL
                     YNVGLVTILAFGPLMLTERGWSFAAGSSATSLVLWIVTISLPAGGLLADRIGRRAVVL
                     VCGLLAFAATMAVAPRVDAVFAIFLLLGITSGLPCGAIMALPTQVLAPETRFVGMGIL
                     FTVYYAVNVGGPWLIGLIAKQAGGARFAFDASAISLGLAGILWLFFRHLASRSPFIEM
                     AEAEG"
     gene            complement(2521..2898)
                     /locus_tag="VFL49_10770"
     CDS             complement(2521..2898)
                     /locus_tag="VFL49_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET7912505.1"
                     /translation="MRREIITTDRIALSVGPFSAAVRAGDLLFLSGQVALDPATGKLI
                     AGDIGAQTEQIFANISAVLEAAGKSFDDVMKTTVYLADMKDFAAMNTVYARYFQTPYP
                     ARTTIQAAGLPLGAAVEIEVVAR"
BASE COUNT          605 a          951 c          894 g          494 t
ORIGIN      
        1 catcgacctc gatgaaggcg ggcaggccgg tcagcttgac gagacggtcc tgcaccggga
       61 tgcccttcca tccctcgagc gtggtcgggc cgatgaagct catcgattcc acatcgaacg
      121 gcccgggcat ggcgagcccg atgccgagca tgcggccgtc cggccgcagc ttgcgcaggc
      181 cggcaaccag cttgccgatc tcccggaatg cgatgtcggg gcgcatctgc ggcaacggcc
      241 gttcgcgcgc gccgatcacc tcgcccgcca ggttgatcag cgccgccttg aggctgatcg
      301 gcgtgacatg gattccgatc gcgaacccgc cttcgggatt gatggcgaga aaggtcggcg
      361 gcaggccgcg gcccctgggc cgcacgcgcg cgcctcggac gaagccttgc agatccagct
      421 cgcggatgat ggtggagacg gtctggacgg tcagcccgac ccgctttgcg atcgcgctgc
      481 gcgcgaccgg cgcgtgcagc cggatcgttt cgagcacgag ccggcggttg tagggccgcc
      541 cgaactcctg gttggttccc ttcaaggcca tgcccgcacc gcccagccgg tctgcgagca
      601 cgctagtccc ggcgcgagga atatgtcaaa cgggcttcaa aaatagcgcg gcggctggcg
      661 ctgcgacaaa agcaggtggc ccaccccagt cgttgtgagc gttctgttac ctatgtctcc
      721 ggactggaca ttgaaaagct tggtgggccc ggcaggactc gaacctgcga caacacccgt
      781 tatgagcggc gcgttctaac cacctgaact aaagccctcc ttcgccaagg cttcggagag
      841 catcctgcct tcgttttgaa gttaggactt gtcctgcgaa gcgcggagcg cgaagcagga
      901 tggtgggccg atcagggtca aactgggcac tggattctct gcgtttcgag ggtatccaat
      961 cgcgcgcgct agtgtgtgac ccggcaacct gaacggcacc gtatgcccac tcggccaagg
     1021 ctctgtctga agtaggttca actcccggaa gggaaaccac cgacagttga ctcttgggcc
     1081 tgtcgcgaga tgacccagtc gcttgggggg ttcggcacgc gggagcgaaa cacgcgttac
     1141 agccccacaa tcgccatcgc aagctaggac attctccaag cacccccctg gctctgcccc
     1201 gcaaacctcc ccgccggcag cgatcgttcc tatcacgcgg ccaaaacgtg gtttttcaac
     1261 acaatccaag catagcggcc cttcattcat taaccctccg cttcggccat ttcgatgaag
     1321 ggcgatcggc tcgcgaggtg ccggaagaac agccaaagga tccccgccag gccgagcgaa
     1381 atcgcgctgg catcaaaggc gaaacgcgcg cctccggcct gcttcgcgat caggccgatc
     1441 agccagggac cgcccacatt cactgcatag tagacagtga acaggattcc catgcccacg
     1501 aaccgcgtct ccggtgcgag cacttgcgtc ggcagggcca tgatggcgcc gcatggcagt
     1561 ccgctcgtga tgcccaacag caggaatatg gcaaagactg cgtcgacgcg gggcgccaca
     1621 gccatcgtcg cggcaaaggc gagtaacccg caaaccagca ccacagcgcg gcgaccgatg
     1681 cgatcggcga gtaaaccacc cgccggcagc gagatcgtca caatccacag cacgagactg
     1741 gtggccgagc ttcccgcggc aaaggaccat ccgcgctcgg tgagcatcag cggcccaaag
     1801 gcaaggatgg tcaccaggcc gacattgtaa agcccataga tcaggcccgc ggttatgaga
     1861 cacacaacga ctcgccgctc cggccaaagc gactgacgtt gagcaacggc gctcgcctcg
     1921 tcgggcgcct tataagccgc tgccaaagct acaaacgccg aaaccagata aaccactacc
     1981 gagatggaag ccagcactcg accgccggaa ctggcgatcc aaggaatcgt ggcaagcgcc
     2041 agcgcaatcc cggctggtgc cgaactgccg atgatcgcca tcgccgtcgc cgtctctttg
     2101 ctggtgaacc aatccgccac catcttgatg atcatgacgt tgagaagaat gccgccggtt
     2161 ccggacacga tccgcgcgcc catcaggatc ggccatgtcg gcgcggtcgc cgcggcgatc
     2221 tcgccgatgg ccataagcgc cagtccaatg agcacaacgg gtttgtcccc aagccatgcg
     2281 ccgatagcgc cacccggaaa cgcgagtatc gtcccggggg gtgtggtaaa gcccgatcaa
     2341 caggccgatg tcagcgatgc tgagcaggaa accggacatc aggtgcggcg aaagcgacgc
     2401 cacagcctga tactggatcc cagttgagac gcgcgccgca aatagaagcg ccaagacgac
     2461 ccagcgattt gggggcatcg aatgttctcg caggtttcgc ccgttccgca tcacttgcgc
     2521 ctatctcgcc acgacctcga tctccacggc ggcgccgagc ggcaggccgg cggcctgaat
     2581 cgttgtccgg gccggatatg gtgtctggaa atagcgcgcg taaaccgtgt tcatcgcggc
     2641 gaaatccttc atatccgcga ggtagacggt ggtcttcatc acgtcgtcga aggacttccc
     2701 tgccgcttcc agcacggctg agatgttggc gaatatttgc tcggtctgag cgccgatgtc
     2761 gccggcgatg agcttgccgg tcgctggatc aagcgcgacc tggccgctga gaaagagcag
     2821 atctccagca cgcacagcgg cggaaaaggg tcctaccgac agcgcgatcc ggtcggtcgt
     2881 gattatctct cgacgcatgt cattgatctc ctgccaaggt taaaatcgtc ggtattgcga
     2941 tcat
//
LOCUS       DASSRL010000530         6126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_678637, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000530 DASSRL010000000
VERSION     DASSRL010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678637"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2227
                     /locus_tag="VFL49_10775"
     CDS             <1..2227
                     /locus_tag="VFL49_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="negative regulator of septation ring formation"
                     /protein_id="HET7912506.1"
                     /translation="LGARALEVADNLDSRIGRFEELLVGRAETVTTQIETRSKAAADA
                     LNARLEQLSEVIKVNSAEAERSLGQLALTTTEAIRASAGDAERALVGVSEEVARNFVG
                     KAEEIAIAVSRRTNEMTTVLSDKSGGVLTAIVEKSEKFTRDITTATDQAVKAIEEKGF
                     AFTRTMMDNSSEISRMINTAGEAATNVVTRTINELNETAQKAIAQSQQTATSAVKEML
                     ETHTMLRSDTTALFERLREANIMLQEVLSGAHENMGALENTLMVRVSEFVTAMNDVTA
                     STGDATGRVETNITAFRDITAQVIADLGQLARQFDMHGRDLVKAVEIIDHTNQRTEDS
                     VNERRVQLDSLVATLDIRTEDLEKRLTRFSGLLDESLEAASTRAREVARLVSETSAEG
                     TRAISEQYERVRENAEEERQRAAEAMRAIYQQTTGETQTLFQNANERFAEAVQGMKQM
                     AAEMQRELETTRNELRRGVFELPQETAESAAQMRRVIVDQIEALAELNRIVARHGRSL
                     DAVEPVRRTREEPTLAIVGSRNEVPRATDTPRSEMPPQRPMQRSENGGFAPPPRRTPA
                     EPPAASPTPAGPGRGWLSDLLTRASREENEPLRELPREPARETARDVTREPSRGSEDR
                     PPRHTIESLDSLSVDIARMIDHDAAADLWDRYKRGERNVFTRRLYTLQGQQAFDDIRK
                     KYRADREFKQTVDRYITEFERLLEEVSRDDRGQVVARTYLTSETGKVYTMLAHAAGRF
                     D"
     gene            2224..3216
                     /gene="dgcA"
                     /locus_tag="VFL49_10780"
     CDS             2224..3216
                     /gene="dgcA"
                     /locus_tag="VFL49_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HET7912507.1"
                     /translation="MTRASPTLSYTVERWPIAGSFGISRGVKTEAVVVVAELTDGRSR
                     GRGECVPYLRYGETVESVVAQIEAIRPEIDAGLNRSALQAAIPPGAARNALDCAFWDL
                     EAKRDSRPVYEIAAIPAPGPLTTAFTISLAAPAEMAKAAAKVASRSLLKIKLGTNGDA
                     DRISAVRGAAPNATLIVDANEGWTEANLGSNLAACEQAGVALIEQPLPAGKDQALARI
                     KRGIPVCADESVHDRASLPALAGKYDAVNIKLDKTGGLTEALAMAADAERQKLAIMVG
                     CMVATSLAMAPAMLLAQSARFVDLDGPLLLSKDRADGLRYEGSLVYPPSAALWG"
     gene            3398..3919
                     /locus_tag="VFL49_10785"
     CDS             3398..3919
                     /locus_tag="VFL49_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912508.1"
                     /translation="MQIRGLVLGAAVMALTVSSASATMRISEDRGGQIGRYLQAFAAV
                     RSSGENVVVDGNCLSACTLLLGLVPRERICATQNARFGFHAAWMPDRDGRPVTSPLGT
                     QALWDIYPASVRRWIKRHGGLSRRMIYLQGRDLSGIVASCDRSPRSQTRSARRVAPES
                     VRYQSASASGERH"
     gene            complement(3851..5014)
                     /locus_tag="VFL49_10790"
     CDS             complement(3851..5014)
                     /locus_tag="VFL49_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912509.1"
                     /translation="MRTEGISALVPRLAIFYAALFVLPGVQMPFFPLWLKAKDVDAGM
                     IGVVLAAPIVARLLAVPVLMREADRRDAVRAALIVCGFAAVAGYVLVGLSAGAVFILL
                     AYAITALATTPQMPLAETYALKELAARKRAYGPVRLWGSFAFIGGNFIAGFAADVIPA
                     RDLIWLIVAASVLIALAAVALPPMTPRAVEASAPDSARGSLLRDRPFVAVIAAASLIQ
                     ASHAEYYGFSAVQWSAAGFDGTVIAALWALGVAAEIVLFALQSRLPPFFGPTGLLLAG
                     ACGAVLRWMGMALDPPAFMLSALQLLHAFSFGATHLGALMFLVRHTPSTHAATAQGHL
                     AVVSGLTMAGAMALSGVLYRDFGSTAYSAMALMAFAGGACGLVAHRLRRDAAR"
     gene            5140..5859
                     /locus_tag="VFL49_10795"
     CDS             5140..5859
                     /locus_tag="VFL49_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912510.1"
                     /translation="MPTETTARAQQLQPGAPDETDYHAFCTALSASARGRAFLAEYAR
                     RNRNADTRPLLTAIERLQFSLTTDPAAPAEMLVKQKLRTLLDDIAAAQSEIEASVIAI
                     RTARLADLIAMVEQRLADIMAPAKDNPVSRHPPAPAEESADEMRAHLAVVPPSDQPEL
                     PIPSPAAAQTPPIVLVRTETPAADVCIAGSQPALPASEQKAAAAAAEPAPPVPLKTSV
                     SPDPLAVIRMLSEEERLALFT"
     gene            complement(5869..>6126)
                     /locus_tag="VFL49_10800"
     CDS             complement(5869..>6126)
                     /locus_tag="VFL49_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7912511.1"
                     /translation="SNTPGELTFPITRELIGQGTTVNDDEVGNAVRYAFHELKLVVEP
                     GGVIGLAALLAGKVAVKGKVVVAILSGANVDPELFGRLIAK"
BASE COUNT         1265 a         1981 c         1909 g          971 t
ORIGIN      
        1 gctcggcgcg cgggcgctcg aggtcgcgga caatctcgac agccgcatcg gaaggttcga
       61 agagctgctt gtcggccggg cagaaacagt caccacccag atcgaaaccc gcagcaaagc
      121 ggcagccgac gcgctcaatg cgcggctgga gcagctcagt gaggtgatca aggtcaattc
      181 tgcggaagcc gagcgctcgc tgggccagct cgccctgacc acaactgagg cgatccgcgc
      241 cagcgcggga gacgccgaac gtgctttggt cggcgtcagc gaggaagtcg cgcgcaattt
      301 cgtcggcaag gccgaggaga ttgcgattgc agtcagccgc cgcaccaacg aaatgaccac
      361 cgtcctatcc gacaagagtg gcggtgtgct cactgctatt gtcgagaaaa gcgagaagtt
      421 cacgcgcgac atcacaaccg ccaccgatca ggcggtcaag gcgatcgagg aaaagggctt
      481 cgctttcacc cgcacgatga tggataacag cagcgaaatt tcccgcatga tcaacactgc
      541 gggcgaagca gcgaccaacg tggtcacccg aacaatcaac gagctcaacg agaccgcgca
      601 gaaggcgatt gcccaatcgc agcagacagc gacttcggcc gtgaaggaaa tgctcgagac
      661 gcacacaatg ctgcgctccg acacgaccgc actcttcgag cgcctgcgcg aagccaacat
      721 catgctgcag gaggtgctca gcggcgctca tgaaaacatg ggcgcgcttg agaacacgct
      781 catggtgcgc gtgtcagaat ttgtcacggc gatgaatgac gtcaccgctt cgaccggcga
      841 cgcgaccgga cgtgtcgaga ccaacattac tgcgttccgc gacatcacgg ctcaggtcat
      901 cgccgatctc gggcagctgg cgcggcagtt cgacatgcat ggccgcgatc tcgtcaaggc
      961 ggtcgaaatc atcgaccaca ccaatcagcg cacggaagac agcgtcaacg agcggcgcgt
     1021 tcagctcgat tccttggtcg cgacactgga catccggact gaggatctcg aaaagcgcct
     1081 gacccgcttc tccggcctgc ttgatgagtc gctcgaggcc gcatccacgc gcgcgcgcga
     1141 ggtcgcccgc ctcgtttcgg agaccagcgc agaagggacc cgcgctatca gcgagcaata
     1201 tgagcgggtt cgcgagaacg cggaggagga gcgtcagcgc gcagccgaag ccatgcgcgc
     1261 gatctaccag caaaccaccg gggaaaccca gactttgttc caaaacgcga acgagcgctt
     1321 tgcggaagcg gtccagggca tgaagcagat ggctgccgaa atgcagcgcg agctggagac
     1381 cacccgcaac gaactgcggc gtggcgtctt cgaactgccg caggaaaccg ccgaaagcgc
     1441 cgcccagatg cgtcgcgtca tcgtcgacca gatcgaggcc cttgccgaac tcaatcgcat
     1501 cgttgcgcgt cacgggcgca gtctcgacgc ggtggagccg gtgcgccgta cacgcgaaga
     1561 gcccaccctc gcgatcgtcg ggagtcgcaa cgaggttccg cgcgcaaccg acaccccgcg
     1621 gagcgagatg ccgccgcagc gcccgatgca gcgaagcgag aacggcggat ttgcaccacc
     1681 tccacgccgt acgcccgcgg aaccgcctgc ggccagcccc accccggcgg gtccgggacg
     1741 tggctggctt tcggaccttc ttacccgcgc ctcacgcgag gaaaacgagc ctttgcgcga
     1801 gctcccgcgt gagccggccc gggaaaccgc gcgcgacgtc acgcgcgagc cgtctcgcgg
     1861 gagcgaggat cggccgccgc ggcacacgat cgagtcgctc gattcgctat ccgtcgatat
     1921 tgcccggatg atcgaccacg acgctgctgc agacctttgg gatcgataca agcgtggcga
     1981 gcgcaacgtg ttcacccgcc gtctctatac gctgcagggt cagcaggcat ttgacgacat
     2041 ccgcaagaag tatcgcgcag accgcgaatt caagcagacg gtcgaccgct acattaccga
     2101 gttcgagcgc ctgctcgagg aagtgtcgcg cgacgatcgc gggcaggttg tggcccgcac
     2161 ctacctcacg tccgaaacgg gcaaggtcta cacgatgctt gcgcacgcgg ccggccgctt
     2221 cgattgacgc gggcgtcgcc gaccctctcg tatactgtcg agcgttggcc gattgccgga
     2281 tcgttcggca tcagccgcgg ggtcaaaacc gaagccgtcg tcgtggtggc cgaactgact
     2341 gacggcaggt cgcgcgggcg cggcgaatgt gtgccctatc tccggtacgg agagactgtc
     2401 gagagcgtcg tcgcacagat tgaagcaata cgcccggaaa tcgatgccgg gctgaaccga
     2461 agcgcactgc aggcggcaat tccgccggga gccgcgcgta acgcattgga ctgcgcgttc
     2521 tgggacctgg aggcgaaacg tgatagccga cccgtgtacg agattgccgc cattccggcg
     2581 cccggtccat tgaccaccgc atttacgatt tccctggcgg cgccggccga gatggcaaag
     2641 gcggcggcaa aagtcgcgag tcgctccttg ctcaagatca agctcggaac caacggtgac
     2701 gccgatcgca tttccgcggt gcgcggcgct gctccaaatg caactctgat tgtcgatgcg
     2761 aacgaaggat ggaccgaagc caacctcggt tccaatctcg cggcctgtga gcaggccggt
     2821 gtcgcattga tcgagcagcc attgccggca ggaaaagatc aggcgttggc acgaatcaag
     2881 cgggggattc ctgtctgcgc cgacgagagc gtccacgacc gcgcatcctt gcccgctctc
     2941 gccggtaagt atgatgcggt caatatcaag ctcgacaaga ccggcggatt gaccgaggcg
     3001 ctggctatgg ccgccgatgc ggagcggcag aaactcgcga tcatggtcgg ctgcatggta
     3061 gcaacgtcat tggcgatggc acccgcgatg ttgctggcgc aaagcgcccg attcgtcgat
     3121 ctcgacggac ctctgctgct cagcaaggac cgcgctgacg gactgcgtta cgagggcagc
     3181 ctcgtctatc cgccttcggc ggcgctttgg ggttgaagcc ctcggaactt ttcgcctgca
     3241 cccgcctgat tctgttcatg aaatgtgccc gatcattcgc gctcattttg tagatttcga
     3301 aaacgaatgc ttcccgttgg acaccggctt ggcttacact tgagccaaac gatcgcaccg
     3361 ctgttcgaac ggcgggcaaa gataacggga cggggaaatg caaattcggg gactggtgct
     3421 tggcgccgcc gtgatggcgc tcacagtatc gtcagcttcc gcaacaatgc ggatttcgga
     3481 agaccgcggc ggtcagatcg gccgttatct gcaggcattc gccgcagtgc gctcatccgg
     3541 cgaaaatgtc gtcgtcgacg gaaattgcct gtcggcatgc acattattgc tgggcctggt
     3601 gccgcgtgag cgcatctgcg ccacccagaa tgcacggttc ggctttcacg ctgcctggat
     3661 gcccgaccgc gacggccgtc ccgtgacaag cccgctcggc acgcaggcgc tctgggatat
     3721 ctatccggca tcggtgcgcc gctggatcaa gcggcacggc ggcctgtcac gaaggatgat
     3781 ctatttgcag ggccgtgacc tgagtgggat cgtggccagc tgcgatcgca gcccgcgttc
     3841 gcagacccgc tcagcgcgcc gcgtcgcgcc ggagtcggtg cgctaccagt ccgcaagcgc
     3901 ctccggcgaa cgccattaga gccatcgccg aataggcggt gctgccgaaa tcacgataca
     3961 gaacgcctga aagcgccatc gcgcccgcca ttgtcaatcc tgacacaacg gcgaggtggc
     4021 cttgcgcagt cgctgcatgc gtggaaggcg tgtgccggac gaggaacatc agcgctccaa
     4081 ggtgcgtcgc gccgaacgaa aaggcatgca gcagttgcag cgcgctgagc atgaaagccg
     4141 gcggatcgag ggccattccc atccagcgca gcacggcgcc gcacgcgccg gccagcagca
     4201 gccctgtcgg cccgaagaac ggcggcaatc tggattgtaa cgcgaacagt acgatctcgg
     4261 cggcaacgcc aagggcccaa agcgcggcga tgacagtgcc gtcgaagccg gcggcgctcc
     4321 actgtaccgc ggagaagccg taatactccg catggctcgc ctggatgagg ctcgcggccg
     4381 ctatgactgc aacgaatgga cggtcgcgca acaggctccc ccgcgccgaa tccggcgcac
     4441 tcgcctcgac cgcccgcggc gtcatgggag gcaaggccac cgctgcgagc gcgatcaaaa
     4501 cgctcgccgc aacgataagc cagataagat cgcgcgcagg aatgacatcc gctgcaaatc
     4561 cagcgatgaa attcccgccg atgaatgcaa acgagcccca gagccgcacc ggcccatagg
     4621 cgcgcttgcg cgcggcgagt tccttgagcg cataggtttc cgcaagcggc atctgcggtg
     4681 tggtcgcaag cgcagtgatc gcataggcaa ggagaataaa gacggcgccg gccgataggc
     4741 cgaccaggac atatccggcc acggccgcaa aaccacaaac gatcaaggca gcgcggacgg
     4801 catcacgccg gtccgcttcg cgcatcagga cgggaaccgc caaaagccgc gcgacgatcg
     4861 gcgctgccag cacgacgcct atcattccgg catcgacgtc cttggctttg agccagaggg
     4921 gaaaaaacgg catctgaacg ccgggcaaaa cgaacaaggc tgcgtagaaa atcgccaggc
     4981 gcgggaccaa agccgaaatc ccttcagtcc gcatcgggtt gtcccaactc caaaccattg
     5041 cacatgctgg cttttttcag cacgccggcc gcctggcgac gcggcggtgc tggccgaatc
     5101 atgcctcatg attgataaac ggctgcgcaa ggattgcaca tgccgaccga gacgaccgcg
     5161 cgggcgcaac aattgcagcc gggtgcgccg gacgagacgg attatcacgc cttttgcacg
     5221 gcgttatcgg caagcgcacg cggccgcgcc tttcttgccg aatatgcgcg gcgcaaccgc
     5281 aatgccgaca cccggccgtt actgacggcg atcgagagat tgcagttctc cctgaccacc
     5341 gatccggcgg ctccggccga gatgctggtc aaacagaaat tacggacgct gctcgatgac
     5401 attgccgcgg cacagagcga aatcgaagcc agcgtcatcg cgatcaggac ggccaggctc
     5461 gctgatttga tcgccatggt cgaacagaga ctcgccgaca tcatggcgcc cgctaaggac
     5521 aatccggtat ctcgccatcc gccggcgccc gcggaagagt ctgccgacga gatgcgcgct
     5581 caccttgccg tggtgccgcc ttcggatcag cccgaattgc caattccgtc acccgccgcg
     5641 gcgcagacgc caccgatcgt gctggtccgg acggaaacgc ccgcggcgga cgtttgcatc
     5701 gccggatctc agccggcgct tccggcctct gaacaaaagg cggctgcagc cgcagccgag
     5761 cctgccccac ccgttccttt gaagacctcc gtgtcaccgg atccgctggc cgtgatcagg
     5821 atgctcagcg aagaggaacg gctcgcgcta ttcacctgag cggccgacct atttggcgat
     5881 cagccggcca aacagctcgg gatcgacgtt cgcgcccgac aggatcgcga ccacgacttt
     5941 ccctttgacc gcgaccttgc cggccagcag cgccgcgagg cctatgacgc cgcccggctc
     6001 gacaacgagc ttgagctcat ggaatgcata gcgcacggca ttgccgacct catcgtcgtt
     6061 caccgtggtc ccctgtccga tcagttcacg cgttatcggg aaggtcaact cgccgggcgt
     6121 attgct
//
LOCUS       DASSRL010000531         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1308760, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000531 DASSRL010000000
VERSION     DASSRL010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308760"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFL49_10805"
     CDS             <1..451
                     /locus_tag="VFL49_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7912512.1"
                     /translation="ARITAFLAVNHAERVRSAILGGLGIRLVEGVGLPESIAEALGAP
                     SLDDVQDPTGRMFRAFAEQTKSDLRALAACIRGSRQTLTRADVGAIRAPVLVAVGTKD
                     LVAGSAQALAALIPGARALDIPNRDHMLAVGDKVYKAGVVDFLNQRA"
     gene            549..1379
                     /gene="cysE"
                     /locus_tag="VFL49_10810"
     CDS             549..1379
                     /gene="cysE"
                     /locus_tag="VFL49_10810"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET7912513.1"
                     /translation="MAQFQARPARGLDKVDPVWARIRSEAEDIARREPELATFIYENI
                     LHHDTLEAAIAHRVSQRLDHADVSADMIRQAYDDALDDQPVLGEAFRADIVATVDRDP
                     ATSRFIEPVLYYKGFHAIQTHRLAHWLYGKGRKDFALYLQSRASSVFQCDINPAAKIG
                     RGIFLDHATGLVVGETAVIEDDVSILHDVTLGGTGKEHEDRHPKIRRGVMIGAGAKIL
                     GNIEVGHCARIAAGSVVIKPVPNNVTVAGVPAKVVGEAGCPEPSRAMDQMLYGIMLDG
                     "
     gene            1452..1634
                     /locus_tag="VFL49_10815"
                     /pseudo
     CDS             1452..1634
                     /locus_tag="VFL49_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
     gene            1626..1862
                     /locus_tag="VFL49_10820"
     CDS             1626..1862
                     /locus_tag="VFL49_10820"
                     /inference="COORDINATES: protein motif:HMM:NF022768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HET7912514.1"
                     /translation="MVIQVGSDPSIQPVPTLNAYLRSKFDNENIRVVPRPKKTDSLEV
                     YIGEEFIAVLFLEVEKGRRSYILELPILDVDLVD"
     gene            complement(1912..2643)
                     /locus_tag="VFL49_10825"
     CDS             complement(1912..2643)
                     /locus_tag="VFL49_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912515.1"
                     /translation="MAFLAQQLSRSARRGNKQRRHAGHNPIMAPVVVFGVVVLLAAFY
                     VAYTLWPRWPQGPVSLDAPSLPIVVSGVTFNVEPAAIRVGVQRRPGVQQRVDLSYLWP
                     SLAPPDPAVKPSVGAPLDPNERLFVTISSGDATLPLMERVQTIYPRYLVPQPVAGFPG
                     LTYRGFRNDSPYQGEDLVFESNAPEHFLARCSRKGVTNSGVCLLERRIGNADITFRFP
                     RDWLPGWENVAKSVDELIARLHPAN"
BASE COUNT          518 a          836 c          855 g          495 t
ORIGIN      
        1 tgcgcgcatt acggcattcc tggcagtgaa ccacgccgag cgggtgcgct ccgcaatcct
       61 cggcggactg ggcatccgtc ttgtcgaagg cgtcggcctg ccggaaagca tcgccgaggc
      121 gctgggggcg ccttccctgg acgatgtcca ggatcccaca ggccgtatgt ttcgcgcatt
      181 tgcagagcag acgaagtccg acctgcgggc gctcgctgcc tgcattcgcg gatcgcggca
      241 gactttgacg cgcgcggacg tcggcgcaat tcgcgcgccg gttctggttg ccgtcggcac
      301 aaaagatttg gtggcgggtt cggcgcaggc cctcgctgcg ctcattccgg gcgcgcgggc
      361 gctcgacatc ccgaaccgcg accatatgct cgccgtcggc gacaaggtct acaaagcagg
      421 cgttgtcgac ttccttaacc aacgcgcatg agccatgcgc gggcggcgct ctcacgctct
      481 ccttgcattc tccaatttgt gctaaaagac ccaggtaggg gttgagaggc ctcgggagaa
      541 ccaggttcat ggctcaattt caggcacggc ccgcaagggg cctcgacaag gtcgatccgg
      601 tgtgggcgcg tatccgcagc gaagcggagg acattgcacg ccgcgagccc gagctcgcca
      661 ccttcattta cgaaaacatt cttcatcacg acacgcttga agcggcgatt gcccaccgcg
      721 tcagccagcg tctggaccac gccgacgtgt cggctgacat gatccgtcag gcttatgacg
      781 acgcgctgga tgaccagccc gtgcttggag aagctttccg tgcggatatt gtcgccaccg
      841 tcgaccgcga tcccgcaacg agccgcttca tcgagccggt gctgtactac aagggcttcc
      901 acgccattca gacgcatcgc ctcgcgcact ggctctacgg caaaggccgc aaggattttg
      961 cgctttacct ccaaagccgc gcgtcatcgg tcttccagtg cgacatcaat ccggcggcga
     1021 aaatcggccg gggtattttc ctcgatcatg caaccgggct ggtcgtcggc gagactgcgg
     1081 tcattgagga cgatgtctcg atcctgcacg acgtgacgct cggcggcacc ggcaaggagc
     1141 acgaagaccg tcatccgaaa atccgccgcg gcgtcatgat tggcgcgggc gcgaagatcc
     1201 tcggcaacat cgaggttggc cattgcgcgc gcatcgccgc gggctccgtt gtcatcaagc
     1261 cggtgccgaa caatgtcacg gttgccggcg tgccggccaa ggtcgtcggt gaggccggct
     1321 gccccgagcc gtcgcgcgcc atggaccaga tgctttacgg catcatgttg gacggctgac
     1381 gcttttgtat tgcgcttgcc ctgcgccctt cgatcgggca ttgaaacggt gcctcaagaa
     1441 ggagtgacgc gttggacgtc caggaagtca gaaaactcga tggctatttg aagaagcttt
     1501 tcggcaatgc gcagatgcgc gtggtgccga aggggcggga caatgccgag gtctttgtgg
     1561 gctccgatga tcttggcgag ctcacggtcg acgacgaaga cggcgagcgc tcctacaatt
     1621 tccgcatggt catccaggtc ggcagcgacc cgagcattca gccggtgccg acgctcaatg
     1681 cctatctgcg cagtaaattc gacaacgaaa atattcgggt cgtcccgcgg ccgaagaaaa
     1741 ccgattcact ggaggtttat atcggcgagg agtttatcgc cgtgctcttc ctcgaagttg
     1801 agaagggccg gcgctcctac attcttgaat tgccgatcct cgacgtcgat ttggtggatt
     1861 gatcaaatcg cgccacctcg tcctggccgg catagcaggc gagcggaacg ttcagttcgc
     1921 cggatgcaat ctcgcgatca actcatcgac gcttttggcg acgttctccc agcccggcag
     1981 ccagtcgcgc ggaaagcgga aggtgatatc ggcattgccg atgcggcgct ccagcagaca
     2041 gaccccggag ttggtcacgc ccttacgcga gcaccgcgca aggaaatgtt cgggcgcgtt
     2101 cgattcaaac accaggtcct cgccctggta cggcgagtca ttgcgaaatc cgcggtaggt
     2161 caatcccggg aaaccggcga ccggttgcgg cacgaggtag cgcgggtaga tcgtctgaac
     2221 ccgttccatc aacggcagtg ttgcgtcgcc ggatgagatc gtgacgaaca ggcgctcatt
     2281 cggatcgagc ggggctccca ctgacggttt gacggctgga tcgggcgggg cgagcgacgg
     2341 ccaaagataa ctgagatcca cgcgttgctg cacgcccggc cggcgctgaa cgccgacccg
     2401 gattgcggcc ggctcgacgt tgaaagtcac gcccgagacg acgatcggga gcgaaggcgc
     2461 atcgagagat accggtccct gcggccaccg cggccacagg gtgtaagcca catagaacgc
     2521 agccagcagc acgacgacgc caaataccac caccggcgcc atgatcgggt tatgaccggc
     2581 atgccgccgt tgcttgttgc ctcgccgggc cgacctggaa agttgctgcg cgagaaaggc
     2641 cacccatcgg ctcctgcgtg cgaatcgcgc ggattatgcc acggcgtacc gcacggagcg
     2701 ggac
//
LOCUS       DASSRL010000532         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_31294, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000532 DASSRL010000000
VERSION     DASSRL010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31294"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="kdsA"
                     /locus_tag="VFL49_10830"
     CDS             complement(<1..656)
                     /gene="kdsA"
                     /locus_tag="VFL49_10830"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET7912516.1"
                     /translation="MNQKPNATVTIGSALFGNDLPLSLIAGPCALESKAHALEMAAAL
                     KDIAGRAGVGFVYKTSFDKANRTSAKSTRGLGLEQALPIFCEIRDKLKIPVLTDVHEA
                     EQCARVAPAVDALQIPAFLCRQTDLLVAAAKTGKAVNVKKGQFLAPWDMPNVVEKITG
                     AGNRNVLVTERGVSFGYNTLVSDMRAIPIMKDEIGAPVIFDATHSVQQPGGQGTSSGG
                     "
     gene            complement(701..1576)
                     /locus_tag="VFL49_10835"
     CDS             complement(701..1576)
                     /locus_tag="VFL49_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7912517.1"
                     /translation="MPRGLDHIVHAVRDLDAAANLYRRLGFTVGARNRHPWGTHNHII
                     QFPGFFIELLTLAEPANLGSDGFSILFGAYNRDFIQRQEGLSVLIVESKDAQADESEF
                     RAADILTAPVMRFEREGKRPDGTGVKLAFSLTFATDKASPDIRFATCQQHYPENFWNP
                     AFQRHGNSAAGIAGVVAVAEHPENQVRFFELFAGKAPNPADDGFTIGTPRGTVDMLTP
                     VAFMRRFGVKPPDVARGPRLAALRFAVGDTSLLQTAPEQAGIAGISAGTPTVIGPADA
                     MGATLVFEAAQAKGR"
     gene            complement(1586..>2837)
                     /locus_tag="VFL49_10840"
     CDS             complement(1586..>2837)
                     /locus_tag="VFL49_10840"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7912518.1"
                     /translation="REGNDGFDFVLCEIGGTVGDIEGLPYFEAIRQLGNDLPRNHAIY
                     IHLTLMPFIPSAGELKTKPTQHSVKELRSIGIQPDVLLCRTDRPIPESERRKLALFCN
                     VRESAVIEARDVDNIYAVPEAYSAEGLDAEVLAAFGIEPRQPPDLDRWRLINERVRSP
                     EGDVTIAIVGKYTGMKDAYKSLIEALSHGGIANKVKVNLDWIESEVFESEDPAPLLEH
                     VDGILVPGGFGQRGAEGKIRAAKFARERHVPYFGICFGMQMAVLEAARNLCGIENANS
                     TEFGATTEPVVGLMTEWLKGNELQKRSASGDLGGTMRLGAYDAILARGSRVSQIYGTT
                     EISERHRHRYEVNTTYKGRLEQRGMRFSGMSPDGILPEIIEYDDHPWFIGVQFHPELK
                     SRPFEPHPLFSSFIGAAVEQSRLV"
BASE COUNT          560 a          861 c          857 g          559 t
ORIGIN      
        1 tggccgccgg acgacgtacc ctgcccgccc ggctgctgca ccgaatgcgt cgcgtcgaag
       61 atgaccggcg cgccgatctc gtccttcatg atcgggatgg cgcgcatgtc ggagacgagc
      121 gtgttgtagc cgaacgagac gccgcgttcg gtcaccagaa cattgcggtt gcccgcgccg
      181 gtgatcttct cgacgacgtt cggcatgtcc catggtgcca gaaattgccc cttcttgacg
      241 ttgacggcct tgccggtctt agcggcagca accagcagat cagtctgccg acagaggaag
      301 gctggaatct gcagcgcatc aacggccggc gcgacccggg cgcactgctc cgcttcatgc
      361 acatcggtga gaacgggaat tttcagcttg tccctgattt cgcagaaaat cggcagggcc
      421 tgctcgagac caagcccgcg cgtggacttg gcggaggtgc gattggcctt atcgaaagac
      481 gtcttgtaga cgaagccgac tccggcgcgc cccgcaatgt ccttcagggc cgccgccatt
      541 tcgagcgcgt gcgccttgct ttcgagcgca cacggtcccg cgatcagcga caatggcaag
      601 tcattgccga agagggccga gccgatcgtg acggttgcgt tgggtttctg gttcaaaggc
      661 tttttcccgc ggcggaccat gaagggcggc cggggtccgg tcaacggccc tttgcctgcg
      721 ccgcctcgaa aaccagtgtt gcgcccattg catcggctgg cccgatcacg gttggagtcc
      781 cggcggaaat tcctgcaatt cctgcttgtt ccggtgcagt ctgcagcagg ctcgtatcgc
      841 caacagcaaa gcgaagggcg gccaggcgcg ggccgcgagc tacatccggc ggcttcacgc
      901 caaagcgacg catgaaggca acgggcgtca acatatcgac tgttccgcgc ggtgtgccga
      961 tcgtgaatcc gtcatcggcc ggattaggcg ctttgccggc gaaaagttcg aagaagcgca
     1021 cctgattctc cggatgctcc gccacggcga caacgccggc aatgccagcc gcgctattgc
     1081 catggcgctg aaaggccggg ttccagaaat tctccgggta atgctgttgg cacgtcgcaa
     1141 aacgaatatc aggcgacgcc ttgtcggtgg caaaggtcag cgaaaaagca agtttgaccc
     1201 ctgtgccgtc cggacgtttg ccctcgcgct caaatcgcat cacaggcgca gtcaaaatat
     1261 cggctgcgcg aaactcgctt tcatccgcct gagcgtcttt cgattccacg atcagaacgg
     1321 agagaccttc ctgtcgttga atgaaatcgc gattataggc cccgaacaaa atcgaaaatc
     1381 cgtcactgcc aagattggcc ggttcggcaa gcgtcagcaa ctcgatgaag aagcccggaa
     1441 actgaataat gtgattgtgc gtgccccaag gatgacggtt gcgcgccccc accgtgaagc
     1501 ctagtcgccg gtacagattg gcggccgcat cgagatcgcg cacggcatga acgatatggt
     1561 cgaggcctcg cggcatcgcg ggtgattaca ccagccggct ctgctcgacc gcagcgccga
     1621 tgaaggatga gaataatgga tgcggctcga atggccggga tttcagctcg ggatggaact
     1681 gcacgccgat aaaccaggga tgatcgtcgt actcgatgat ctcgggcaaa atgccatcgg
     1741 gcgacattcc ggagaaacgc atgccgcgct gctcaagtcg ccctttatac gtggtattga
     1801 cttcatagcg gtgtcgatga cgctcggaaa tttccgtcgt cccgtagatt tgcgacacgc
     1861 gactgccgcg cgcgaggata gcgtcatacg cgccgaggcg catcgtgccg ccaaggtcgc
     1921 cgctcgcact gcgtttctga agttcattgc ccttgagcca ttccgtcatc aggccgacga
     1981 cgggctcggt cgtggcgccg aattcagtcg aattggcgtt ttcgatgccg cacagattgc
     2041 gggccgcttc aagcaccgcc atctgcatcc cgaaacaaat gccgaaatac gggacgtgtc
     2101 gctcgcgcgc gaatttcgcc gcgcggatct ttccttccgc gccgcgctgg ccgaatccgc
     2161 ccggcacgag aatgccgtcg acgtgctcca gaagcggcgc cggatcctcg ctttcaaaaa
     2221 cctcagactc gatccagtcg agattgactt ttaccttgtt ggcaatgccg ccgtgcgaaa
     2281 gcgcctcaat cagcgatttg taggcgtcct tcatgcccgt gtatttgccg acgatggcga
     2341 tagtgacgtc gccctcagga cttcggaccc gctcattgat cagacgccaa cggtcgagat
     2401 cgggcggttg ccgtggctcg atgccgaatg cggcgagcac ctccgcatcg agcccttcgg
     2461 cggaataggc ttcgggcact gcatagatgt tgtcgacgtc gcgcgcctcg atgacagcgc
     2521 tctcccgcac attgcagaac agcgcgagct tcctccgttc gctttccggg atcggccgat
     2581 cggttcggca cagcaagacg tccggctgaa tgccgatgga gcgcaattcc ttgaccgaat
     2641 gctgggtggg cttggtcttg agctctcctg cgctcggaat gaacggcatc agcgtgagat
     2701 ggatgtagat cgcatggttg cgcggcagat cattgccgag ctggcggatc gcctcgaaat
     2761 acggcaggcc ttcgatgtcg ccgacggtgc cgccaatctc gcagaggaca aagtcgaaac
     2821 catcattacc ttcacgg
//
LOCUS       DASSRL010000533         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1711485, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000533 DASSRL010000000
VERSION     DASSRL010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711485"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFL49_10845"
     CDS             <1..942
                     /locus_tag="VFL49_10845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7912519.1"
                     /translation="RASVSQAQLTKISVGYSAISGDALPAWLAKDSGIFEKNGLDVQL
                     VFFTGGTTAVMALVSSDTPIAQLAGAAVVNSVLAGSDAALIAGGVTSLNYYLLGRPDI
                     KTPEQLKGGSVAISRFGSSSDFIARYALQKIGLTPGKDVTIVQIGSTTARVDAALTGR
                     VQATVVNPPASIIAQKRGMTVLADLPKLGLVYQHTAVATSRKYIREHPDVVRRYVKSQ
                     LEVVHRIYTDKEAAIRALGRFIGRTVERDVLEKTWENLLSESVLPKKQYPSLEGIKTI
                     LATEAKGKPGKPEDFVDMSFVKEFDQSGFTDGLYKKR"
     gene            complement(1103..2818)
                     /locus_tag="VFL49_10850"
     CDS             complement(1103..2818)
                     /locus_tag="VFL49_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HET7912520.1"
                     /translation="MGEQQIRPAASFLNYTPVPLAFGTSGLRGLVKDITDLEAYINVK
                     GALRYLLSIGDIHARSTVVVAGDLRPSTERIMRACVQAIIDSGCEVENAGTIPTPALI
                     SHAISARRAGVMVSGSHIPFDRNGIKINKSIGEVLKSDESGISHEVERVRAEAYARSA
                     TTSPFDTRGMLKRAPDLPALNRAAEEAYVRRYLSSFDSGGLSGVRVLFYQHSAVGRDI
                     LPRILRELGAEVVTAGRSDTFIPVDTENITGEQLDRLEELASAAEASGQPLHAIASTD
                     GDSDRPLVTAVLPAAEVKPGGRRVRFLPGDLLGLVVAEYLRADAAAVPISANDAVERR
                     FHERKILLQKTRIGSPYVVAALDELRGPPTRERIVGWEANGGFLTGSEISLSAGTLAA
                     LPTRDSTLPILANLFAAAEERIGLSTLWERLPARFGCAGLLDNFPLAASQAILTQLIP
                     PGDAIEVAFDGAGQVVDRTRAGAEGAPLSPATAEAWRQSKTKLTRVFTPALGFDAIVR
                     INVLDGVRVYFHNGDVAHVRPSGNAPQLRIYANSDSQARADRIVELGLREPDGILRRL
                     ERAFT"
BASE COUNT          583 a          870 c          871 g          525 t
ORIGIN      
        1 cgggcgagtg tgagccaggc tcagctaacc aaaatcagcg tcggctatag cgccattagc
       61 ggcgatgccc tgcccgcatg gctcgccaaa gattccggca tttttgaaaa gaacgggctc
      121 gatgttcaat tggtcttttt caccggcggc accaccgccg tgatggcgct ggtttcctcc
      181 gacacaccga tcgcccagct tgccggcgcg gcggtggtca attccgtgct cgcgggttcc
      241 gatgccgcgc tgatcgccgg cggcgtgact tcactgaatt attatttgct cggtcgaccg
      301 gacatcaaga ccccggagca actcaaaggc ggttcggtgg cgatcagcag atttggctcg
      361 tcgtcggatt tcatcgcccg ttatgcgctg caaaaaatcg gccttacccc tggaaaggac
      421 gtgaccatcg tgcagatcgg cagcaccacc gcgcgggtgg atgccgcctt gaccggtcgc
      481 gtgcaagcaa ccgtggttaa cccgccggcg agtatcatcg cgcagaagcg cggcatgacg
      541 gtcttggccg atttacccaa gctcggcttg gtttatcaac acacggcggt ggcgacgagc
      601 agaaaatata ttcgcgagca tcccgacgtc gtgcgccgct acgtcaaatc gcagctggaa
      661 gtggttcatc gcatttacac cgacaaagaa gcggcgatcc gcgctctggg gagatttatc
      721 ggccgcaccg tcgagcgcga cgtgctggaa aagacctggg aaaacctgct gagcgaatcg
      781 gtgctgccga aaaagcagta cccgtcgctc gaaggcatca aaacaattct ggcaaccgaa
      841 gcaaaaggca aaccgggcaa gccggaagac ttcgtcgata tgagcttcgt caaagaattc
      901 gatcaaagcg gctttaccga cgggctctat aaaaaacgct gatcggtaag gtcgccgccg
      961 cgctttgctt ccgcgctctg gacgcggtgc ggtaattcat gcagccggca acctcaggcc
     1021 attgcgagca gctcgatggg caaggaaacg acagccgcac tgacacacag ccccgttttc
     1081 cccaccagca catggcggtc gcttacgtaa aggctcgttc caggcgacgc aagattccgt
     1141 cgggttcgcg caagccaagc tccacgatcc ggtcggcgcg cgcttgagaa tcgctgttgg
     1201 catagatgcg cagttgcggc gcgttccccg acggcctaac gtgcgcgacg tcgccgttgt
     1261 gaaaatagac acgcacgccg tcgagcacgt tgatgcggac gatggcgtcg aaaccgagcg
     1321 ccggcgtgaa gacgcgcgtt agctttgtct tggactgccg ccacgcttcg gccgtggccg
     1381 gcgaaagcgg cgcgccctcc gcaccggcgc gagtccgatc gactacctgc cctgcgccgt
     1441 cgaacgcgac ctcgattgcg tcaccaggcg gaatcagttg agtgaggatc gcctggctcg
     1501 cggcaagcgg gaagttatcg agcaaaccgg cgcatccaaa gcgcgccggc agccgctccc
     1561 acaacgtgga caatccaatc ctttcctcag ccgcagcgaa cagattggcc aaaatcggca
     1621 aggtggaatc gcgcgtcggc aacgccgcca gcgtgcccgc gcttaatgaa atttcgctgc
     1681 cggtcaaaaa tccgccgttg gcttcccagc cgacgattcg ctcgcgggtt ggcggcccgc
     1741 gcaattcgtc gagggcagca acgacgtaag gcgagccgat tctggtcttc tgcaataaaa
     1801 ttttgcgctc gtggaagcga cgctcgaccg catcgttggc gctgatcgga accgcagccg
     1861 cgtctgcgcg cagatactcg gcgacgacga gacccaggag atcgccgggc aaaaaacgca
     1921 cgcgccgccc gccgggctta acttcggcag cgggtagaac ggccgtgact aatggccgat
     1981 cggagtcgcc gtcagtggag gcgatggcat ggagcggctg gccgctcgct tcggcggcgg
     2041 aggcgagctc ttccagacga tccaattgct cgccggtgat gttttcggta tcgacgggaa
     2101 tgaacgtgtc actgcgcccc gcggtgacca cctcggcgcc gagttcgcgc aggatgcgcg
     2161 gcaagatatc gcgtcccacc gccgaatgtt gataaaacag aacccgcacg ccggagaggc
     2221 cgccgctgtc aaagctgctc aggtagcggc ggacgtaagc ttcttccgcg gcgcgattaa
     2281 gcgccggcaa gtccggcgcc cgtttcagca ttccgcgggt gtcgaagggc gatgtcgttg
     2341 cgctccgtgc gtacgcctcg gcgcgcacgc gctcgacctc gtggctgatg ccggattcgt
     2401 ccgatttgag tacctcgccg atgcttttat tgatcttgat gccgttacgg tcaaatggaa
     2461 tatggctgcc gctcaccata acaccggcgc gcctggcaga aatcgcgtga gagatcagcg
     2521 cgggggtggg aatcgtgcct gcattctcga cctcgcagcc cgagtcgata atcgcctgaa
     2581 cacaggcgcg catgatgcgc tcggtgctgg gccggaggtc gccggccacg accaccgtgc
     2641 tgcgcgcatg gatatcgccg atgctcaaca aataacgcag cgcacctttg acgttgatat
     2701 acgcttcgag gtcggtaata tccttgacca acccgcgcaa accgctggtg ccaaaggcca
     2761 acgggaccgg cgtataattg agaaacgagg ccgcgggccg tatttgctgc tcgcccattg
     2821 gagttttcga ttatgctaaa ttcataagt
//
LOCUS       DASSRL010000534         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1588734, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000534 DASSRL010000000
VERSION     DASSRL010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588734"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="secD"
                     /locus_tag="VFL49_10855"
     CDS             <1..1129
                     /gene="secD"
                     /locus_tag="VFL49_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7912521.1"
                     /translation="QSVEQSIQIIERRVNELGTVEPLIQRQGVDRILVQVPGLQDPTR
                     LKELLGKTAKLDFRMVDVSMPAEQALQGRVPPDDEILYSSTQPRTPYLVEKRILVSGG
                     DLTDAQPGFDQRTSEPVVNFRFNTSGARKFAQATQENVGRPFAIILDNQVISAPVIRE
                     PILGGQGQISGNFTVQSANDLAILLRAGALPAPLTIIEERTVGPGLGQDSIAKGKLSS
                     YVGAAMVIIFMLLTYGLFGLFANIAVAINVAMIFGILSLLNATLTLPGIAGIVLTVGI
                     AVDSNVLIYERIREEVRGGRTPINAIDAGFTRALATILDSNITTFIAAAVLFYIGTGP
                     VRGFAVTLGIGIITTVFTAFTLTRLIVAYWVRWRRPQRVPI"
     gene            1187..2077
                     /gene="secF"
                     /locus_tag="VFL49_10860"
     CDS             1187..2077
                     /gene="secF"
                     /locus_tag="VFL49_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7912522.1"
                     /translation="MRFRRISFPMSALLSIIAISLYFFHGLNFGIDFKGGTLMEVQSK
                     VGDADLAKMRATLGALGLGEVQLQQFGAPNDVLIRIAEQPGGETAQQDAVRKVRGALG
                     DQVEYRRVEVVGPRVSGELLSYGVVGLMLAIVGILIYLWFRFEWQFALGAMIANVHDI
                     VLTIGFMSVTQIDFDLTSIAALLTILGYSLNDTVVIYDRIREMLRRYRKMPMPDLLNA
                     SVNQTLSRSVITHVTVSLALLALLLFGGQAIHSFTATMMFGVVLVGTYTSVFIASPIL
                     IYLGVGTGRDSLSAPDRDKR"
     gene            2074..2460
                     /locus_tag="VFL49_10865"
     CDS             2074..2460
                     /locus_tag="VFL49_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HET7912523.1"
                     /translation="MTVGGSDAPHLPQPAPIEAYGDGGFRFAGMSHRGSLLCFPDGIW
                     AWPVVAPDQLDERRLAAAFDRAQALDFFLIGAGRDPWPLPGDLRQRFRDLSLSVDTMP
                     TGAAVRTYNILLAENRRVGAGLIAVA"
     gene            2483..>2635
                     /locus_tag="VFL49_10870"
     CDS             2483..>2635
                     /locus_tag="VFL49_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004683706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HET7912524.1"
                     /translation="MDKNDEYCAALVREADRDRHLATLFAPADRRGALFSLYAFDIEI
                     SRIRELA"
BASE COUNT          430 a          848 c          810 g          547 t
ORIGIN      
        1 tcagtcggtc gagcagtcga tccagattat cgagcgccgc gtcaacgaac tcggcaccgt
       61 tgagccgctg atccagcgcc agggggtcga tcgcattctg gtgcaggttc cgggtctgca
      121 ggacccgacg cggctgaagg agttgctcgg caagacggcc aagctcgatt ttcgcatggt
      181 ggacgtatcg atgccggccg agcaggcgct gcaggggcgc gtgccgcccg acgatgaaat
      241 tctttacagc tcgacgcagc cgcggacgcc ttatctggtc gaaaagcgga tcctggtgtc
      301 gggcggcgat ctgaccgacg ctcaacccgg ctttgatcag cgcacgagcg agccggtcgt
      361 caatttccgt ttcaatacat ccggcgcccg caagttcgcc caggcgacgc aggaaaatgt
      421 cggcaggccg ttcgcgatca ttctcgacaa ccaggtgatc tccgcgcccg tcattcgcga
      481 gccgatcctc ggcggccagg gccagatttc gggcaacttc accgttcaga gcgccaacga
      541 ccttgccatt ctgctccggg ccggcgcatt gccggcgccg ttgaccatca tcgaggagcg
      601 cacggtcggc ccgggccttg ggcaggactc gatcgccaaa ggcaaacttt cttcttacgt
      661 tggcgcagcc atggtgatca tcttcatgtt gctgacctat ggcctgtttg gactgtttgc
      721 gaacattgcc gtggccatca atgtcgccat gatcttcggc atcctgtcgc tgctcaacgc
      781 gacactgacc cttcccggca tcgccggcat cgtgctcacg gtgggtattg ccgttgattc
      841 caacgtgctg atctacgagc gcatccgcga agaagtgagg ggcgggcgaa cgccgatcaa
      901 cgccatagac gctggattta cccgtgcgct tgcgaccatt ctcgattcga acatcacgac
      961 gttcatcgcc gccgcggtgc tgttctatat cggcaccggc ccggtgcgcg gctttgccgt
     1021 gacgctcggg atcggcatta tcacgacggt cttcaccgcc ttcacgctga cgcggctgat
     1081 cgtcgcctac tgggtgcgtt ggcggcgccc gcaacgcgta ccgatctgag gactccgcgc
     1141 cgtgcgcctg cttcgcatcg ttcccgacga taccaaattc gacttcatgc gctttcggcg
     1201 catcagcttt ccgatgtcgg cgctgctttc gatcatcgcg atttcgcttt atttcttcca
     1261 tggactgaat ttcggcatcg atttcaaagg cggcactttg atggaggtgc agtcgaaggt
     1321 cggcgacgcc gaccttgcaa agatgcgcgc gacgctcggt gccttgggcc tgggcgaagt
     1381 gcagttgcag caattcggcg cgccgaacga cgtgctcatc aggatcgccg agcagccggg
     1441 cggagaaacc gcgcagcagg acgcggtccg caaggtgcgc ggcgcgctcg gcgaccaggt
     1501 cgagtaccgg cgcgtcgagg tcgtcggccc gcgcgtctcc ggcgagctcc tgtcctacgg
     1561 cgtggtcggc ctgatgctcg cgatcgtcgg aatcctgatc tacctgtggt tccgcttcga
     1621 atggcagttc gcgctcggcg cgatgatcgc caacgtgcac gacatcgtgc tcaccatcgg
     1681 cttcatgtcg gtcacgcaga tcgatttcga cctgaccagc atcgccgcgc tgctcacgat
     1741 tctcggctac tcgctcaacg acacggtcgt catctacgac cgtatccgcg aaatgctgcg
     1801 gcggtaccgc aagatgccga tgccggattt gctcaacgca tcggtcaacc agaccctgtc
     1861 gcgctctgtc attacccacg tcaccgtgtc actcgcgttg cttgcattgc tgctgttcgg
     1921 cgggcaggcg atccacagct tcaccgccac catgatgttc ggcgtggtgc tggtcggcac
     1981 ctatacctcg gtgtttatcg cgtcgccgat cctgatctac ctcggcgtcg gcaccggccg
     2041 ggattcgctg agcgcgcccg accgggacaa gcgatgacgg ttggcggcag cgatgcgccg
     2101 catctgccgc agcctgcacc catcgaggcc tatggcgatg gcggatttcg ttttgccgga
     2161 atgtcgcatc gaggctcgct gctttgcttc cccgacggca tctgggcctg gccagtcgtc
     2221 gctccggatc aactggacga gcgcagactc gctgcggcct ttgaccgggc gcaggccctc
     2281 gattttttcc tgataggggc gggccgcgat ccatggcctt tgcctgggga cctgcgccag
     2341 cgtttccgtg atctgtccct gtcggtcgac acgatgccga ccggggcagc ggtgcgcacc
     2401 tacaacatcc ttttggccga aaaccgccgt gtcggggcag ggctgatcgc cgtcgcgtga
     2461 cgcgcgcggt agactcttcc ggatggacaa aaacgacgaa tattgcgcgg cgctggtgcg
     2521 cgaagccgat cgcgatcggc acctggcgac actgtttgcc ccggccgacc ggcgcggcgc
     2581 gctgttctcg ctctacgcct tcgacattga aatctcgcgc attcgggagt tggca
//
LOCUS       DASSRL010000535         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_644478, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000535 DASSRL010000000
VERSION     DASSRL010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644478"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /gene="secG"
                     /locus_tag="VFL49_10875"
     CDS             complement(<1..167)
                     /gene="secG"
                     /locus_tag="VFL49_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7912525.1"
                     /translation="MQHVIIVIHLMLVLALIGVVLLQRSEGGGLGIGSTGGFMTSRGT
                     ANVLTRATAILA"
     gene            complement(291..1049)
                     /gene="tpiA"
                     /locus_tag="VFL49_10880"
     CDS             complement(291..1049)
                     /gene="tpiA"
                     /locus_tag="VFL49_10880"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7912526.1"
                     /translation="MAAAKIIPLVAGNWKMNGLAASESELAKIVVGVGSLNGKADIMV
                     CPPATLIAAFAAAARGSSVVIGAQDCHAEPSGAHTGDVSAEMLADAGARAVIIGHSER
                     RADHHETDAQVRAKTQAAWRAGLTAIVCIGEQRAERQAGRTLQVVGSQLDGSLPDGAT
                     AANLVVAYEPVWAIGTGLTPTAADIAEVHAFIRGRLSARVASPAASVRILYGGSVKPD
                     NAGQLLTVPNVNGALVGGASLKAADFLGIAAIYR"
     gene            1214..>2018
                     /locus_tag="VFL49_10885"
     CDS             1214..>2018
                     /locus_tag="VFL49_10885"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7912527.1"
                     /translation="MLRGLHKASSTWLGKAVMALVMGFLVVSFAIWGIGDIFRGFGRN
                     AVATIGGTEISVEQFRQFYNDRLQQLGRQAGRPITPDEARARGLDRQLLAQLIAETTI
                     DEQAKQLRLGIDDAEIAKRITSDPSFRGANGQFDRARFEQIIRQAGYTESRFIEEQRR
                     SMLRRQLAQSITGDLKVPATALAALNQYQNEKRQIEYIALGAEQAGEVPEPTPEVLNK
                     YFDERKTLFRAPEYRKITLLPLAPADLAKPDAVTDADAKAYFEQHKDSYG"
BASE COUNT          406 a          671 c          580 g          361 t
ORIGIN      
        1 gccaggatcg ccgtcgcgcg cgtgagcacg ttcgccgtgc cgcggctcgt catgaaaccg
       61 ccggtcgagc cgataccgag cccgccacct tctgaacgct gcagcagcac cacgccgatc
      121 agggcgagaa caagcatcag atggatgacg atgatgacgt gttgcatcag tccaaaatcc
      181 gttcaaaccg aaccattccg ggcttcccgc ccgggcggcg ccatccggct atataggaat
      241 tcgccgcggt ctctacacga tcatccggcg tttttccacc ccgacccgcg ctagcggtaa
      301 atcgccgcaa ttccgagaaa atcggcggct ttcaagctcg cgccgccgac cagggcgcca
      361 ttcacattag gcaccgtcaa aagctgcccg gcgttgtccg gcttgaccga gccgccatac
      421 agaatgcgca ccgacgcggc aggcgacgcg acgcgcgcgc tcagtcggcc acggataaac
      481 gcatgaactt cagcgatatc cgccgccgtc ggtgtaaggc cggttccaat tgcccagacc
      541 ggttcgtacg caaccaccag attggccgca gtcgcaccat ccggcagtga gccgtcgagc
      601 tggctgccga caacctgcaa ggttctcccc gcctgccgtt cggcacgctg ttccccgata
      661 cagacgatgg cagtcaaccc ggcgcgccag gccgcctgtg tcttcgcccg cacctgcgca
      721 tcggtctcat ggtggtcggc gcgccgctcg gaatggccga taattaccgc acgtgcgccg
      781 gcgtccgcca gcatttcagc cgaaacatca cccgtgtggg cacctgaagg ctcggcgtga
      841 cagtcttgcg cgccgatgac tacggacgag ccgcgcgcag ccgcggcaaa tgcggcaatc
      901 aacgtggccg gcggacaaac catgatatcc gccttgccgt taaggctccc gacgcccacg
      961 acaatttttg ccagctcgct ttcggaggcg gccagtccgt tcattttcca attgcccgcc
     1021 accagcggga taatcttggc tgccgccatc caatcgccct ctcccgcgtg cggtatcaga
     1081 cccgcctggg caaagccagt ggcccggatt ggcgctgatc cttcttgagt ctctatggtg
     1141 cgcccgccgt gcttgggtta gacatgcgcg gtcattcacc cccgattatt gcaaggcatg
     1201 gaatagcgcg cgcatgcttc gtggtcttca caaagcctcc tcgacctggc tcggcaaggc
     1261 cgtaatggcc ctcgtgatgg gttttctcgt cgtcagtttc gcgatctggg gcatcgggga
     1321 catcttccgc ggcttcgggc gcaacgcagt ggccacgatt ggcggaaccg aaatatctgt
     1381 cgagcaattt cgccagttct acaatgaccg cctgcagcaa ttgggacgtc aggccggacg
     1441 cccgattacg cccgatgagg cgcgcgcccg cggactcgat cgccaattgc tcgcccagtt
     1501 gattgccgag accacgatcg atgagcaggc caagcagttg cggcttggca tcgacgacgc
     1561 cgaaatcgcg aagcgtatta catcagatcc gtcgttccgt ggtgcgaatg ggcagttcga
     1621 ccgtgcgcgc tttgagcaga tcattcgtca ggcaggatat accgagagcc gcttcatcga
     1681 agagcagcgc cgttcgatgc tgcgcaggca actcgcgcag agcatcacgg gcgacctgaa
     1741 ggttcctgca accgctttgg ctgcactcaa ccagtaccag aacgaaaagc ggcagattga
     1801 atatatcgcc ctgggagcag agcaagccgg agaggtcccg gaaccgacgc cggaagtcct
     1861 gaacaagtac ttcgacgaac ggaagacgct gttccgcgcg cccgaatacc gcaagatcac
     1921 attgctcccc ctcgccccgg ctgatctcgc aaagcccgat gccgtcacag atgccgacgc
     1981 caaagcgtac ttcgagcaac ataaggacag ctacggca
//
LOCUS       DASSRL010000536         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_224390, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000536 DASSRL010000000
VERSION     DASSRL010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224390"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFL49_10890"
     CDS             <1..451
                     /locus_tag="VFL49_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7912528.1"
                     /translation="GYVTDRIANLLRIRGFSHVLVDLGEQRALGPKRDGTPWVVVRHR
                     LREIELRNGALATSEGAGCILGAKGAVHHLFDPRTGRSSHRWRALTVHHDSAAIADAL
                     STALYSAARDEITTLVNKFDGLIVWASDHSGRESRWDSTVRATGTGS"
     gene            complement(448..1530)
                     /locus_tag="VFL49_10895"
     CDS             complement(448..1530)
                     /locus_tag="VFL49_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET7912529.1"
                     /translation="MSKTDLRLVRLWLLAAAVMVLLTLMVGGATRLTESGLSIVEWKP
                     ITGVLPPFSEPEWQAEFAKYQTIPQYQQLNQGMSLGAFKVIYWWEWSHRLLARLTGIV
                     FLVPFLFFLWCGAIPARLRARLWGIFGAGAALGAVGWWMVSSGLTQGVAVSQYRLAFH
                     LTLASAIYAAILWTAQQLKMHGRREESPARIRLSALAIATLLLLQIYLGALVAGLNAG
                     LIYNTWPTIDGAIVPASKNLLFLQPAWRNLFENTLMVQFNHRIVAYAIWILAVAHAFD
                     AWQTARAVWGAMVLAGAVTLQALLGVATLLYQAPLALALAHQVLAIFVLTIAVVHAER
                     LSVAAMSSVRHAKPAGQAADREVAAA"
     gene            complement(1548..2180)
                     /locus_tag="VFL49_10900"
     CDS             complement(1548..2180)
                     /locus_tag="VFL49_10900"
                     /inference="COORDINATES: protein motif:HMM:NF025244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HET7912530.1"
                     /translation="MRDIGTRFTNVISAIDAVNAEDPNPVKIDGKKFPAELVYGYRMS
                     ETLDRMHPAASECLRIAARGQHIGRWRQPRKNYPQGRNGYLEWRRYQRNCQAKRLGDL
                     MADSGYEADDIARVGSLIKKKHLRIDADAQMLEDVICITFLEHYLFGFRARVDDDKLA
                     GILAKSWKRMSERGHKYALQLNLPEEVTRLLERGLNALDATNPAASASKH"
     gene            complement(2222..2593)
                     /locus_tag="VFL49_10905"
     CDS             complement(2222..2593)
                     /locus_tag="VFL49_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HET7912531.1"
                     /translation="MVAALLCAGSMTAGLAQDLGAGANSFKKCAPCHDVGETAKNKVG
                     PLLNGLGGRKTGNIAGYNYSDANKNSGIVWDEDTFLEYIKDPKAKIPGTKMVFAGIKN
                     ESEAKNLWAYLNQFAADGKKK"
     gene            complement(2748..3146)
                     /locus_tag="VFL49_10910"
     CDS             complement(2748..3146)
                     /locus_tag="VFL49_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET7912532.1"
                     /translation="MALLQWKEHYSVGIDAVDHEHKELIALINRLHNQLSAKGDELSI
                     HAFFGDLYAGISAHFALEERFMRERHYDQLSQHKADHERLLDEIRDIMDDFEGHENAS
                     VIGPRLDAWFSRHFETHDARLHKALGPHPE"
     gene            complement(3159..3659)
                     /locus_tag="VFL49_10915"
     CDS             complement(3159..3659)
                     /locus_tag="VFL49_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912533.1"
                     /translation="MIYLRYAGQFVVIAALFVVVTAFSDWPTYRQVPRDSAILMLSFV
                     HGADRKAECRRLTPEEIQKLPPNMRRVQECPRGRRPIYVELDLGGRTIFSANLPPTGI
                     AGDGPSKVYERFVVPAGQHDVAVRMRDTPRTDGFDHERHAAVTLVADQMFVIDFRAET
                     GEFIFR"
     gene            complement(3656..>4182)
                     /locus_tag="VFL49_10920"
     CDS             complement(3656..>4182)
                     /locus_tag="VFL49_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HET7912534.1"
                     /translation="GIDLPDYSLKTLRERTIAAAAALSGPNRVLVFACDHGGGRRVEG
                     AVRMPCVAMAPPSLFDFVLSRDLADGLVIAGCAESACYNRLGLAWTKQRIAGTRDPYL
                     RARVPRDRIATIWASELEGRRVAAEISAFAKEIAELPRSALRLPPSLESISKPVASVR
                     PGSDRVPTEAGGAK"
BASE COUNT          892 a         1257 c         1170 g          863 t
ORIGIN      
        1 aggctatgtg acggacagaa tcgccaatct gttacgcatt cgcggtttca gtcatgtgct
       61 ggtcgatctg ggcgaacagc gtgcgctggg gcccaagcgg gacggaacac cttgggtggt
      121 ggttcgccac cggctccggg agatcgagct cagaaatggc gcactggcaa cctccgaagg
      181 cgccggctgc attttgggcg ccaagggcgc ggtccatcat ctgtttgacc cccgcactgg
      241 gcgcagctct catcggtggc gagctttaac cgtccaccac gactccgccg ccattgccga
      301 tgccttgtcg accgctctct attccgctgc ccgcgacgag atcacgaccc tggtcaataa
      361 atttgatggg ctgatcgttt gggcgagcga ccacagtggg cgtgaaagcc gatgggattc
      421 gactgtccgt gcgactggca ccggatccta agctgctgca acctcgcgat ctgcggcctg
      481 ccccgccggc ttcgcatgtc gcacggaaga catcgcagca acggacagcc gctccgcatg
      541 caccacggca atggtgagca cgaaaattgc aaggacctga tgcgcgagag caagcgcaag
      601 cggcgcctga tagagcagcg tggcgacacc gaggagagcc tgcaatgtca ccgctccggc
      661 caaaaccata gcgccccaga cggcgcgggc ggtttgccat gcatcgaagg cgtgcgcgac
      721 cgcaaggatc cagatcgcgt aggcgactat gcggtgattg aactgaacca tcagcgtatt
      781 ttcgaacaaa ttgcgccagg ccggttgcag gaacaataga tttttcgatg cagggacaat
      841 ggcgccgtcg atcgtcggcc aggtattgta aatgagacct gcattaaggc ctgcgaccaa
      901 tgcaccgaga taaatctgca acagcagcag cgttgcaatg gccaatgcgc tgagccggat
      961 gcgtgccggg ctctcctccc tgcgcccgtg catcttcaat tgctgggccg tccacaggat
     1021 cgctgcgtag attgcgcttg ccagcgtgag atggaacgca agccggtatt gcgaaacagc
     1081 cacgccctgt gtgaggccag aagaaaccat ccaccagccg acggcaccaa gcgcagcacc
     1141 tgcgccgaaa attccccaca ggcgcgcacg caagcgcgcg ggaattgcgc cacaccagag
     1201 aaaaaataga aaaggcacga gaaacacgat cccggtcaat cgtgccagca ggcgatgcga
     1261 ccattcccac caatagatca ccttgaaagc acccaggctc ataccctgat taagttgctg
     1321 gtactgcggg atcgtttggt acttggcgaa ttcggcctgc cattctggct ccgaaaatgg
     1381 cggcagcacg ccagtaatgg gcttccactc cacgatagat agtccggact ccgtcaaccg
     1441 cgttgcgccg ccaaccatga gtgtcagaag gaccattacg gccgccgcaa gcagccaaag
     1501 ccgcaccagg cgaaggtcag tttttgacat tttcgctgtg tcagtcatca gtgcttgctt
     1561 gcggaagccg ccggattcgt cgcatccagc gcattcagac cacgctccaa caaccgtgtt
     1621 acctcttccg gcagattcaa ttgcaacgcg tacttatgcc cgcgctctga cattcttttc
     1681 caggatttgg ccagaattcc tgccaacttg tcatcgtcga cccgggccct gaaaccgaaa
     1741 aggtagtgct caagaaaagt aatacagatg acatcctcaa gcatttgagc gtcggcatcg
     1801 attctcaagt gcttcttctt gatcaggctt ccgacccgcg caatatcatc ggcttcgtag
     1861 cctgagtccg ccatgagatc gccgagacgc ttcgcctgac aattccgctg atatctgcgc
     1921 cattcgagat atccgttccg tccctgggga tagtttttgc gcggctgccg ccagcggccg
     1981 atatgctggc cgcgcgctgc aattcgcaaa cattccgatg cggcaggatg catgcgatcc
     2041 agcgtttcac tcatccgata tccatagacc aactcagccg gaaacttttt cccgtctatc
     2101 ttgacgggat tggggtcctc ggcgttgacc gcatcgatcg cggatatcac atttgtaaag
     2161 cgtgtcccga tatcgcgcat ggctggcccg gaaatgaact caatccgaaa gccgcaaatt
     2221 actacttctt ttttccatcg gcggcgaact ggttaagata ggcccaaaga ttttttgcct
     2281 cgctttcgtt cttgatgccg gcaaacacca ttttggtgcc gggaatcttt gcttttgggt
     2341 cctttatgta ttcgagaaaa gtatcctcat cccatacgat gccggaattc ttgttggcgt
     2401 ccgaatagtt atatccggcg atatttccgg tcttgcgacc gccgaggccg ttcaacaatg
     2461 ggccaacctt gttttttgcc gtttcgccga catcgtggca aggcgcacat tttttgaatg
     2521 aattggctcc ggcaccgaga tcttgcgcga gccccgccgt catggagccc gcacagagca
     2581 gtgcagcgac cattagtttc gatttaatca tctctgaact ttcctctaga tgcatccgcc
     2641 aaggtgctgc aaggcgcagc gcttgttggt agttgcttcc gggcaagatc ggcttgacct
     2701 tggtcaagcc gatcagtttt tcttcaggac acaaaagcgg ccccagttta ctccggatgc
     2761 ggaccaagcg ccttgtgcag acgtgcgtcg tgcgtctcaa agtgtcgcga gaaccatgcg
     2821 tcaagccgcg gaccaatcac gctggcgttc tcatgcccct cgaagtcgtc catgatgtcg
     2881 cgaatttcgt cgagcagacg ttcgtgatcg gctttgtgct ggcttagctg gtcataatgg
     2941 cgctcgcgca tgaaacgctc ctcaagcgca aagtgagcag aaatgccggc atataagtca
     3001 ccgaaaaacg catggattga aagctcatca cctttcgccg agagttggtt gtgcaaccgg
     3061 tttataagcg cgatcagctc tttgtgctcg tgatcgacgg catcgatgcc gacgctgtag
     3121 tgctctttcc actgcaagag agccatggcg gttgctcctc atcgaaatat gaattcgcca
     3181 gtctcggcgc gaaaatcgat tacgaacatc tggtccgcga ccagcgtgac cgcggcatgc
     3241 cgctcgtgat caaatccgtc tgtccgcggc gtgtcgcgca tgcgcaccgc aacgtcatgc
     3301 tgccccgcag gcacgacaaa gcgttcatag accttcgaag gtccgtcgcc cgcgataccg
     3361 gttggcggca ggttcgcgct gaagatcgtc cggccaccga gatcgagctc gacatagatc
     3421 ggacgccgcc cgcgcgggca ttcctgtacg cggcgcatgt tcggtggaag cttctgaatt
     3481 tcttccgggg ttagacggcg gcattcggcc ttgcgatccg cgccgtgaac gaagctgagc
     3541 atcaagatgg ctgagtcccg tggaacctgg cggtaggtcg gccaatccga gaacgctgtg
     3601 acgacgacaa acaacgccgc gattacgacg aattgtccgg cataccgcag ataaatcatt
     3661 ttgcgcctcc ggcttccgtc ggcactcggt cgcttcccgg acgcaccgaa gccaccggct
     3721 tcgaaatcga ctccaatgac ggcggcaatc gcaacgccga acgcggcagt tcggcgattt
     3781 ccttcgcgaa cgctgaaatc tctgcagcaa cgcgcctgcc ttccagttcg gaggcccaaa
     3841 tcgtcgcgat acggtcgcgc ggaacgcgcg cacggagata gggatcgcgt gtccctgcga
     3901 tgcgttgctt ggtccaggcc aacccaagcc gattgtagca ggcgctctct gcgcacccgg
     3961 caatgacgag gccgtcggcc aggtcgcggc tcagcacgaa atcgaacagc gagggtgggg
     4021 ccattgcaac gcagggcatg cggacagcgc cctctactcg ccggccaccg ccatgatcgc
     4081 acgcgaagac caagacccgg ttcggtccag aaagcgctgc cgccgcggca atcgtccgct
     4141 cgcgcagcgt cttgagactg tagtcaggca gatcgatacc gg
//
LOCUS       DASSRL010000537         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_836588, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000537 DASSRL010000000
VERSION     DASSRL010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836588"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..932)
                     /locus_tag="VFL49_10925"
     CDS             complement(174..932)
                     /locus_tag="VFL49_10925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET7912535.1"
                     /translation="MALRALLFRRTSEPKGITVAFDGEVYPVRLRRHGQARRYTLRIH
                     AASREVVLTMPPRGSVKQAREFAQKNGAWIATRLRRLPDAVPFAHGTRLPLRGIDHLI
                     AHRNGARGTVWTEAGAGMPLLCVAGEAPHVHRRVHDYLKREAKRDLEAASRTAAQALS
                     VSIKRVSVRDQSSRWGSCSSTGVLSYSWRLILAPPFVLDYLAVHEVAHLIEMNHSRHF
                     WRLVEKVCPHVGRAKAWLDAHGADLHRYGVSENF"
     gene            complement(999..2342)
                     /gene="phaZ"
                     /locus_tag="VFL49_10930"
     CDS             complement(999..2342)
                     /gene="phaZ"
                     /locus_tag="VFL49_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HET7912536.1"
                     /translation="MAIGEFGGAATLSAGGGAFLATPMYWFYELGHAALNPSRAIADA
                     TRLFFRNPANPLAHTAYGKSVAATMELFERSTRRYRRPEWGIESTTVGGEKVAVNIKS
                     VWQRPFCRLLHFERAFVHPPRRPQPKLLLVAPMSGHYPTLLRGTVETFLPNHDVYITE
                     WIDARMVPLAEGRFDLDSYIDYVISMLHALDGDVHVIAVCQPSVPVMAAVALMEADND
                     PDVPHSMVLMGGPIDTRINPNAVNDLAERRGLNWFRNHVITKVPFPHPGMMRDVYPGF
                     LQLHGFMSMNLDRHIEAHHNLFLHLVKGDGDSAQKHREFYDEYLAVMDLTAEFYLQTV
                     DTVFIRHALPKGEMTHRDRRVDPAAIKRVALMTVEGEHDDISSVGQTEAAHALCVNIP
                     AEDKVHYLQPSVGHYGVFNGSRFRAEIAPRIADFVLSHNGVGAVSAAPLRVVARH"
     gene            complement(2479..3297)
                     /locus_tag="VFL49_10935"
     CDS             complement(2479..3297)
                     /locus_tag="VFL49_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912537.1"
                     /translation="MSGTGSGMEFSLNRVGAMVRRYWYLLRSSWPRILDLIYWPTVQM
                     LMWGFLQVYISQNAGFFARAAGVFIGSVLLWDILFRGQLGFSISFLEEMYARNLGNIM
                     MSPLRPVEFLLALMIMSVVRLSIGMIPVSILAIVFFGFNLWALGIALAAFFANLILTS
                     WSVGIFVAGLLLRNGLGAESMAWTIMFLFLPLTCVYYPVAVLPEWLQYVAWLLPPTYV
                     FEGMRSLVIDHVFRADLMAQAFALNVVLFAAASLAFFKLVESARIHGSLLQTGE"
     gene            complement(3294..4052)
                     /locus_tag="VFL49_10940"
     CDS             complement(3294..4052)
                     /locus_tag="VFL49_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912538.1"
                     /translation="MDAQQTPAIAVDRLVKRYKGVEAVDGISFRLARGSITGLLGGNG
                     AGKTTTIAMIMGLVTPTSGEVSVLGAEMPRERYRVLHRMNFESPYVDMPMRLTVRQNL
                     SVFAQLYAVSDIDGRIASLAGDLDLTEFLDRPTGKLSAGQKTRVSLAKSLLNRPEVLL
                     LDEPTASLDPDTADWVRSRLEQYQAESGATVLLASHNMNEVERLCERVIIMKKGRIED
                     DDTPAHLLARYGRETLEEVFLDVARGRRQSEAAQ"
     gene            4154..>4929
                     /locus_tag="VFL49_10945"
     CDS             4154..>4929
                     /locus_tag="VFL49_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HET7912539.1"
                     /translation="MRALGPAQHHPHRNVRLDTLVRLRWLAIIGQTTAVLVVHYGLDF
                     QLPVYACLGVIALAAWLNVALRLRFHITQRLEPDRAAWLLAFDIAEMAVLLFLTGGLQ
                     NPFAFLFLGPVLLSATALPPRFTLILGGFAVVCSTILVFVHYPLPWDIEDPLQLPPVY
                     MMGVWLCILLAIGYIGVYAWQITEESRQLSDALAATELVLAREQHLSQLDGLAAAAAH
                     ELGTPLSTISVIARELERAIAPNAPHGDDVRLLREQATRCR"
BASE COUNT          978 a         1571 c         1488 g          892 t
ORIGIN      
        1 cgtaagctgc acctgcgcgg aggaggcgcc gagctgccgc gcgatgtccg gcagcgacgg
       61 caggtacatg tcggtcgtca gcgggccgac cgcggtcaaa gcagccagca gagccgtgag
      121 cgcgaaagtt cctggacgca gcatggacag gtatttccaa aggatttttt aggtcagaaa
      181 ttttcgctca cgccataacg atgcaaatcg gcaccgtgcg catcgagcca ggcctttgcg
      241 cgccccacat gcggacaaac tttctccacc agccgccaga aatgccgcga gtgattcatc
      301 tctatgagat gtgcgacctc gtgcaccgcg aggtagtcca gcacaaacgg cggcgcgagg
      361 atcaaccgcc acgagtatga aagcacgccg gtcgacgagc atgaacccca ccggcttgac
      421 tgatcgcgca ctgacacgcg cttgatgctg acgctaagcg cctgcgccgc tgtccggctc
      481 gccgcctcca ggtcgcgttt ggcttcccgc ttgagatagt cgtgcacccg ccgatgcaca
      541 tgcggcgcct cgccggccac gcacagaagc ggcatgccgg cgccggcttc ggtccagacc
      601 gtgccgcgcg ccccgttacg atgggcgatg agatggtcga tgccgcgcag cggcaagcgt
      661 gtcccgtgcg cgaaaggcac ggcgtccggc aggcgccgca gacgcgtcgc gatccatgct
      721 ccgttctttt gcgcaaattc tcttgcctgc ttgacgctgc cgcgcggcgg catcgtgagc
      781 acgacttcgc ggctggcggc gtggatccgc agcgtgtatc gccgcgcctg gccatgacgt
      841 cgaaggcgga ccggatagac ctcgccgtca aatgcgacgg ttattccctt cggttcgctg
      901 gtgcggcgaa aaagcagggc gcgcagcgca atggacatct tccacccaga atcagagggt
      961 gacggcagtg tgccataccc gcctgcgcga acgcgagatc aatgccgggc tacgaccctc
     1021 agcggcgcag cgctcactgc gccgacaccg ttgtgcgaaa gcacaaagtc ggcgatgcgg
     1081 ggcgcgatct cggcacggaa gcgcgaaccg ttaaacacgc cataatggcc gacgcttggc
     1141 tgcaggtaat gaaccttgtc ttccgccggg atattgacgc acagcgcgtg cgcagcctcg
     1201 gtctgcccta cactggagat atcgtcgtgc tcgccttcga cggtcatcag cgcaacgcgc
     1261 ttgatggcag ccggatcgac gcggcgatcg cgatgcgtca tttccccttt cggcagggcg
     1321 tgacggataa acaccgtatc gaccgtctgc aggtaaaatt cggccgtcag gtccatgacg
     1381 gccagatact cgtcgtaaaa ctcgcgatgc ttctgggctg aatcgccgtc gcctttgacc
     1441 aggtgcagaa acaggttgtg gtgcgcctcg atatgccggt cgaggttcat gctcatgaag
     1501 ccgtgcagct gcagaaaacc gggatagaca tcgcgcatca tccccggatg cggaaacggg
     1561 accttggtta tgacgtggtt gcggaaccag ttgagcccgc gccgctccgc cagatcgttg
     1621 acggcattcg ggttgatccg cgtatcaatc ggcccgccca tcaataccat cgagtgcggg
     1681 acatccggat cgttgtcggc ttccatcagc gcgaccgccg ccatcacagg taccgacggc
     1741 tgacacacgg cgatcacatg aacgtcgccg tcgagcgcat gcagcatcga aatgacgtag
     1801 tcgatgtagg aatcgaggtc gaaccggcct tcggcgagcg gcaccatgcg cgcatcgatc
     1861 cactcggtga tgtagacatc atgattcggc aggaatgtct cgaccgtgcc gcgcaacaac
     1921 gtcggataat gtcccgacat cggcgcgacg agcagaagtt tcggctgcgg ccggcgcggc
     1981 ggatgcacga acgcgcgttc gaaatgaagc agacggcaga acggacgttg ccagacgctc
     2041 ttgatgttca ccgctacctt ttcgccgccg acggtggtcg actcgattcc ccattccggc
     2101 ctgcggtagc ggcgggtcga gcgctcgaac agctccatcg tcgccgcgac tgattttccg
     2161 taggcggtat gagcgagcgg attggcaggg ttcctgaaaa acaggcgcgt ggcatcagca
     2221 atcgcgcgcg acggattgag cgcggcatgc cccagctcgt aaaaccagta catcggcgtc
     2281 gcgaggaacg ccccgccacc tgcggaaagc gttgccgctc cgccgaattc acctattgcc
     2341 attgagtccc ccgaccctcg ctgcggtgcg gcagactgtc gtttttcgtc catagcgtca
     2401 acatagagat aagttcttcg cgtctggttt acaacgcgct gggcccgatt aagtctcagt
     2461 gaacacaagc ttttttagtc actcaccggt ctgcaacagt gagccgtgaa tccgcgcgct
     2521 ctccacaagc ttgaaaaatg ccaatgacgc agctgcgaac aggacgacgt tcaacgcaaa
     2581 ggcctgggcc atgaggtcgg cgcgaaacac atggtcgatc accagcgaac gcataccttc
     2641 gaaaacgtaa gtcgggggca gcagccaggc gacgtattgc agccactccg gcagcaccgc
     2701 gaccgggtag tagacgcacg tgagcggaag aaagagaaac atgatggtcc atgccatgct
     2761 ctcggcgccg agaccgttcc gaagcaaaag tccggcgaca aaaataccga cggaccagct
     2821 cgtcaggatc agattcgcga agaaagccgc cagcgcaatg ccgagggccc acagattgaa
     2881 accgaaaaac acgatggcca ggatggaaac cggaatcatt ccgatcgaca ggcggacgac
     2941 gctcatgatc atcagcgcca gcaggaattc caccgggcgc agcggcgaca tcatgatgtt
     3001 gccgaggtta cgcgcgtaca tttcttcgag aaaagaaatc gaaaagccga gctggccgcg
     3061 gaacaggata tcccacaaca acaccgagcc gatgaatacg ccggcggcgc gcgcgaagaa
     3121 gccggcattc tgtgaaatgt agacttgcag aaagccccac atcaacatct gcacggtcgg
     3181 ccagtagatc aggtcgagaa tgcgcggcca cgacgagcgc agcagatacc agtagcgccg
     3241 caccatcgcg ccgacgcggt taagcgaaaa ctccattccg ctgccggtgc cactcattgc
     3301 gcggcctcgc tttgtctccg gccgcgggcc acgtcgagaa acacttcctc cagggtttca
     3361 cggccatagc gcgcgagcag atgcgccggc gtatcgtcgt cctcgatccg gccctttttc
     3421 atgatgatca cgcgctcgca caaacgctca acctcgttca tgttgtgcga ggcgagcaga
     3481 acggttgcgc cgctttcagc ctggtattgc tcgagccgcg agcgcaccca atccgccgtg
     3541 tccggatcga gcgaagcggt cggctcgtcg agcaacagaa cctcgggtcg gttgagcagc
     3601 gacttggcca gcgatacgcg cgtcttctgg ccggccgaaa gcttgccggt cggccggtcg
     3661 agaaattcgg tgagatcaag gtctccggca aggcttgcga tacgcccgtc gatatcggac
     3721 acggcgtaga gctgcgcgaa gacggaaaga ttctgacgca ccgtcaggcg catcggcatg
     3781 tcgacataag ggctctcgaa attcatccgg tgcagcacgc ggtagcgttc gcgcggcatc
     3841 tcggcgccaa gcacgctgac ttcgcctgac gtcggggtca caagccccat gatcatcgcg
     3901 atcgtcgtgg tcttgcccgc accgttgccg ccgagcagcc cggtgattga acccctcgca
     3961 agcctgaagg atatgccgtc taccgcctct acgcccttgt agcgcttgac caggcggtcg
     4021 acggcaattg cgggcgtttg ctgcgcgtcc atgacgcgaa gagatgtgac gccccgccgc
     4081 agaaagcgca agggccgatt cccgcgtcgt ctgcgcactt ctacagagca agccgagact
     4141 ttacactggc gccatgcgtg cgctcggacc tgcccagcat cacccgcacc gcaatgtgcg
     4201 tctcgatacg ctcgttcggc tgcgctggct tgccatcatc ggccagacga ccgcggtgct
     4261 cgtggtccac tatggcctcg acttccagtt accggtctat gcctgcctcg gcgtgatcgc
     4321 cttggcggcc tggctcaacg tcgcgctacg gctccgcttc cacatcacgc aacgtcttga
     4381 gcccgaccgc gcggcctggc tgctcgcgtt cgacatcgcc gagatggcgg tgctgctgtt
     4441 tctgaccggg gggctgcaga atccctttgc attccttttt ctcggtccgg ttctcttgtc
     4501 cgcgaccgcc ctgccgcccc gcttcacgtt gatcctcggc ggatttgctg tcgtctgctc
     4561 cacgattctt gtcttcgtgc actatccgct gccttgggat atcgaagacc cgctgcagct
     4621 gccgccggtc tacatgatgg gagtctggct ctgcatcctg cttgcgatcg gatacatcgg
     4681 cgtctacgcc tggcagatca ccgaagagtc gcgacagctt tcggacgcgc tggccgcaac
     4741 cgagctggta ctggcgcgcg agcagcatct ttcgcagctt gacggcctcg ccgctgccgc
     4801 tgcgcatgag ctcggcacgc cgctctcgac catttcggtg atcgcaaggg agctcgagcg
     4861 cgcgatcgca cccaacgcgc cacacggcga cgacgtgcgg cttttgcgcg agcaggcgac
     4921 gcgctgccg
//
LOCUS       DASSRL010000538         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1396513, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000538 DASSRL010000000
VERSION     DASSRL010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396513"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFL49_10950"
     CDS             complement(<1..1012)
                     /locus_tag="VFL49_10950"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HET7912540.1"
                     /translation="MYTFGMEEEYFLFDAKTRHAVRRANKDFLSLAQEQLGDHVMTEM
                     LQSQIEVATPPCATMDEARTHLSHYRQTLAKAAASHQLGIAAMGTFPLAFWPDQMVTR
                     KARYGAIMDDLQMIGYRNMLCGMHVHVAVPDVDTRINLILRLTPYLPLLLALSTSSPF
                     WQAHLTGLVGYRLAAYDELPRTGLPELFRTNEDYREFISALTWAKIIPDASYIWWALR
                     PSLNNPTIELRVADSCTRLEDAVAIAALFRCLVRALDRDRALNAGFDRVGRAITQENK
                     WHAQRYGIAATFVEPFSRSPLTLAQWLDQVIDFIAEDIEALDCAAEIRHLHAIAAKGT
                     SGD"
     gene            complement(1137..1907)
                     /locus_tag="VFL49_10955"
     CDS             complement(1137..1907)
                     /locus_tag="VFL49_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET7912541.1"
                     /translation="MTSISRFLAAVCAVMLTLAAPASSNADELSASQRSEVERIVREY
                     LIAHPEVLQEAMNELDKRQTAAQAEKHKSAIKQYSEALFSSPRQVVLGNPSGNVTFVE
                     FFDYNCGYCKRAMDDMLTLLKDDPKLKVVLKEFPVLGPGSVEAAQVAVAVRMQDKTGK
                     KYLEFHQKLLGGRGEANKARALAVAKDIGLDMTRLDKDMASPEVKATLQESFKLAEAL
                     GLNGTPSYVIGDNVVVGAVGLDALKEKVNTSRCGKPNC"
     gene            complement(1926..3350)
                     /locus_tag="VFL49_10960"
     CDS             complement(1926..3350)
                     /locus_tag="VFL49_10960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7912542.1"
                     /translation="MSLDGTPIRTQRRPPAAQAVALVTALAVGLAGVAPARAQDSSTR
                     GMPIVRDAEIEQLLRDYAQPILRAAGLAKQNVKVVVLRDRSFNAFVMDGRHIFINAGA
                     LFDAKTPNEVIGVFAHETGHLAGGHLQRLREQLASAQTASIIALLAGLGAAVAGSRSG
                     AGGADIGAAAIMAPQQAIMRTMLAYVRTQEDQADHAGVKFLNATGQSPRGMVDLFKRL
                     SNESLFNSRYIDPYMQTHPMPAERVAALETIARASPYWDRKDPPELQLRHDMMRAKLS
                     GFLERPDTVARRYPASDHSLPARYARAISTYRHSDLRQAIAQMDSLIQAQPNNPYFYE
                     LKGQALLEGGHPSEAVAPLRHAVQLSHNNALIEILLAQALNATGNAKLSEEAVAMLRS
                     AIVREPEAPDAYSQLAVAYGRKGDLANADLASAQAAFTRGDIKTARQLAARAKTRLPV
                     GSPAWVRADDIVNVKPPNANAGLH"
     gene            3520..>4211
                     /locus_tag="VFL49_10965"
     CDS             3520..>4211
                     /locus_tag="VFL49_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7912543.1"
                     /translation="MLDVAKKLLTPSKRSDVPPFIVMDVMAAAAQREAQGASIIHMEV
                     GQPAAPAPATAIKAAQTALAQGRIGYTETLGIPSLRARIARHYKDAYDFALDPARIVV
                     TTGSSGAFILAFLSLFEPGDRVALANPGYPPYRHILSALGCEPVLIETTAETRWALSA
                     KALVAAHQRKPLKGVVIASPANPTGTMMDADTLARLIAVAEAEGIRVVSDEIYHGLDY
                     AFAAETAANVSDR"
BASE COUNT          759 a         1289 c         1372 g          791 t
ORIGIN      
        1 ggtcgccgct cgtacccttg gcggcgatgg catgcagatg cctgatttct gctgcgcaat
       61 cgagcgcctc gatgtcctcc gcaatgaagt cgatgacctg gtcaagccat tgcgcgagcg
      121 tgaggggaga acgggagaac ggctcgacaa aggtcgcggc gataccatag cgctgcgcat
      181 gccatttgtt ctcctgtgtg atggcgcggc cgacgcgatc gaaaccggcg ttgagcgcgc
      241 gatcgcggtc gagcgcgcgc acgaggcacc ggaaaagggc agcgattgcg acggcgtcct
      301 caagccgcgt gcaagagtcg gcgacacgca gctcgatggt cggattgttg agcgaagggc
      361 gcaaagccca ccagatgtag ctcgcgtccg gaatgatctt tgcccaggtc aaggcggaga
      421 tgaattcgcg atagtcttcg ttggtacgga atagctcggg caatccggtg cgcggcaact
      481 catcgtaggc tgcgagccgg tagccgacga ggcccgtcag gtgagcttgc cagaacggcg
      541 aagatgtcga gagcgcaagc agaaggggca gatagggtgt gaggcgcagg atcaggttga
      601 tgcgcgtatc gacatccggc actgcgacgt gcacgtgcat cccgcataac atgttgcggt
      661 aaccgatcat ctgaaggtcg tccataatgg cgccatagcg ggccttgcgc gtcaccatct
      721 ggtcaggcca gaaagcgagg gggaacgtgc ccatggcggc aatgccaagc tgatgactgg
      781 cggcggcctt cgcgagagtc tgccggtagt gactgagatg ggtccgcgcc tcatccatcg
      841 tggcgcatgg tggtgtggcg acctcgatct gcgattgcag catctcggtc atgacgtgat
      901 cgccaagctg ctcttgcgca agcgaaagga aatccttgtt cgcccgacgc accgcgtgac
      961 gcgtcttggc gtcgaacagg aaatattctt cttccatccc gaacgtgtac atgcgcgcga
     1021 caaccgcccc gccggaactt tgttccgttg ctgtttttga cgaaaaaacg gccgccggga
     1081 ggctaccacg gcggccggac tcctaaggac ggagtattac aggatgttgg cgcccctcag
     1141 cagttcggct tgccgcagcg cgaggtgttg accttttcct tgagcgcatc gaggccgaca
     1201 gcgccgacta cgacgttgtc gccgatcacg tagctgggcg tgccattgag gccgagcgct
     1261 tcggcgagct tgaagctttc ctgcagggtt gccttcactt ccgggcttgc catgtccttg
     1321 tcgagccgcg tcatgtcgag gccgatgtcc ttggcgaccg ccagcgcccg cgccttgttc
     1381 gcctcgccgc ggccgccgag cagcttctga tgaaattcga ggtatttctt gcccgttttg
     1441 tcctgcatcc gcaccgcgac cgcgacctgc gctgcctcga ccgatccggg tccgagcacc
     1501 gggaattcct tcaagaccac cttgagcttg ggatcgtcct tgagcagcgt gagcatgtca
     1561 tccatggcgc gcttgcagta gccgcagttg tagtcgaaga actccacgaa agtgacgttg
     1621 ccgctcgggt tgccgagcac aacctgccgc ggcgagctga acaaggcttc cgaatattgt
     1681 ttgatcgccg acttgtgctt ctccgcctgc gcggctgtct gccgtttgtc gagctcgttc
     1741 atcgcctcct gaagcacttc aggatgggcg atcaggtatt cgcggacgat gcgctcgacc
     1801 tcgctgcgct gactggccga gagctcatca gcgtttgaac tcgccggcgc cgcgagcgtc
     1861 aacatgacgg cacatacggc cgcgagaaag cgcgagatcg atgtcatggt tcttgatcct
     1921 tgtgactaat ggaggcctgc gttggcgttg ggcggcttga cgttgacgat atcgtcggcc
     1981 cgcacccatg cgggagagcc gacgggcaga cgggtcttgg cacgcgctgc aagctgacgc
     2041 gcggtcttta tgtcgccgcg cgtgaacgcg gcctgcgcgg aagcgaggtc ggcattggcc
     2101 aggtcgcctt tgcggccgta ggccaccgcc agttgcgagt aggcatccgg cgcctccggc
     2161 tcgcgcacga tcgcggagcg cagcatggcg accgcttcct cggaaagttt cgcgttgccg
     2221 gtggcattga gtgcctgtgc cagcaggatt tctatgaggg cattgttgtg cgacagctgg
     2281 accgcgtgcc gcagcggagc cacggcttcc gacggatggc cgccttcgag gagcgcctga
     2341 cccttgagct cgtagaagta cgggttgttc ggctgcgcct gtataagcga atccatttgc
     2401 gcaattgcct gccgcagatc ggaatgccgg taggtcgaga tcgcgcgtgc gtaacgggcc
     2461 ggcagactgt ggtcactggc cgggtagcgc cgtgcgaccg tgtccggccg ctccaggaag
     2521 ccggaaagct tggcgcgcat catgtcatgg cggagctgca gctcgggagg atccttgcgg
     2581 tcccagtacg gactggccct ggcgatcgtt tcgagtgcgg cgacgcgttc agccggcatc
     2641 ggatgggtct gcatgtaggg atcgatgtaa cgcgaattga acaatgattc gttgctcagc
     2701 cgtttgaaca aatcgaccat gccgcgcggc gattgccctg tcgcgttaag gaatttcact
     2761 cctgcgtgat cggcctgatc ctcctgcgtg cgcacatagg cgagcatcgt gcgcatgatc
     2821 gcctgctgcg gcgccatgat tgcggccgcc ccgatgtcgg cgccgccggc gccggaacgt
     2881 gaaccggcga ccgccgcgcc gagcccggcc agcaaggcaa tgatcgatgc cgtctgcgcg
     2941 gaggcaagct gctcgcgcaa tcgctgcaag tgcccgccgg caaggtgtcc ggtctcgtgg
     3001 gcgaacacgc caatgacttc gttgggggtc ttggcgtcga acagcgcacc ggcattgatg
     3061 aagatgtgcc ggccatccat gacgaaagcg ttgaacgagc ggtcgcgaag gaccaccacc
     3121 ttcacattct gctttgcaag tccggccgcc cgcaggatcg gctgtgcgta gtcgcgcagc
     3181 aattgctcga tctccgcgtc gcggacgatc ggcattccgc gtgtcgaact gtcctgcgca
     3241 cgcgcgggcg caacgcccgc caggccgacc gcaagagccg taaccaaggc gaccgcctga
     3301 gccgctggcg gcctgcgctg cgtcctgata ggtgtgccgt ccaaggacat gccgatgtgc
     3361 accgaatgcg cgtgaaacta ttgacgcccc tacaggcggt caacgcggcc tgcgatcacg
     3421 accaggtcat gtttcgagtc atgacggcga cggccgcaag caaaccgggg aatgcggcgg
     3481 cagcggggca gggaaacaag gtctaagcgg ccaaaatcga tgcttgatgt ggcaaaaaag
     3541 ctcctgaccc cgtcgaaacg cagcgatgtg ccgccgttca tcgtcatgga cgtcatggcg
     3601 gccgcggcgc agcgcgaggc gcaaggcgcc agcatcatcc acatggaggt agggcagccg
     3661 gcagcgccgg ctcctgcaac agcaatcaag gctgcgcaga cagcgctggc gcagggacgc
     3721 atcggctaca ctgaaaccct gggcattcca tccctgcgtg cccgcattgc tcgccactac
     3781 aaggatgcct acgattttgc tctcgatcca gcgcgcatcg tcgtgacgac gggttcttcc
     3841 ggcgcgttca ttctggcgtt cctgtcactg ttcgagccgg gcgaccgtgt cgccctggcc
     3901 aatcccggct acccgccgta tcgccacatc ctttccgcgc tcggctgcga gccagtgctt
     3961 atcgaaacaa ccgccgagac gcgctgggct ctgtcggcga aagcgctggt cgcggcgcat
     4021 cagcgcaagc cgctcaaggg cgtcgtgatt gcaagtccgg caaatccgac cggcaccatg
     4081 atggatgcgg acacgctcgc ccggctgatc gcggtggcgg aagcggaagg catccgtgtg
     4141 gtctccgacg aaatctatca cgggctcgac tacgcctttg ccgccgaaac cgcggccaac
     4201 gtctcggacc g
//
LOCUS       DASSRL010000539         4110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1169351, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000539 DASSRL010000000
VERSION     DASSRL010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169351"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFL49_10970"
     CDS             <1..271
                     /locus_tag="VFL49_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912544.1"
                     /translation="RAVLLLKPGGRIAYVTCSVLEEENGVQVRAFLARHAAFKTVPPD
                     QVAQALGERAFMFCKAARLSSEGILMTPRLTDTDGFFVAVLRRGG"
     gene            397..1350
                     /locus_tag="VFL49_10975"
     CDS             397..1350
                     /locus_tag="VFL49_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912545.1"
                     /translation="MLVARRDPPFHVDARDAVSANFETGSKPPRVVRFVTVIQMIGSL
                     VAVPVGIGSAYTFYRANFSPETTCQSLRSGIIAMLDKGVDASTRRVLVRRDVETFEKT
                     CATVDPEATAAFKTLLAAEKSAAPIPAVVPAPKAQPSETVSKEPVHKAEPRPQPAPKQ
                     AVTNAQPPAAQSTRKEPVSDTQWLDAVRQALVTHQESARPAEGVKARPAAAPSIRPAQ
                     REVEPAAPVVAPAPVATSTPAASAAAPVLPPPVAVVPTPAQQNAVRTQDPDHPVPPQS
                     IPDAPEVAAAETKADEPGRSKIGKWISAIPLLGPVIDNGRH"
     gene            complement(1347..2246)
                     /gene="aat"
                     /locus_tag="VFL49_10980"
     CDS             complement(1347..2246)
                     /gene="aat"
                     /locus_tag="VFL49_10980"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7912546.1"
                     /translation="MVFETERMTLPAATRGRADRRAALFRENLAGVAQRHILGLAWAL
                     SPARIRGLPNVLRLCFNELLAPDYALPDPQRAFASPPGLAGVVHDLGRPTLLAAYRRG
                     LYPFAHVAPLKWWSPPFRSVLFFNELHIAKRLRRQMRQHNYTVTLDSDFEAVIAACAA
                     HRDGRWHLTWITPRIMRAYADLFDAGYAHSFEVWNERGELAGGGYGVAIGGVFVTESQ
                     FSREPNTSKMGFSALNWHLARWGFALNDGKLMTPTCRDMGFREIPREDYLVRLAQSGR
                     QADKPGRWQVETDLTTVADWQPN"
     gene            2434..3981
                     /gene="guaA"
                     /locus_tag="VFL49_10985"
     CDS             2434..3981
                     /gene="guaA"
                     /locus_tag="VFL49_10985"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7912547.1"
                     /translation="MHDKVLIVDFGSQVTQLIARRVREEKVYSEIVPYQKAETAFREM
                     KPKAVILSGGPASVLDKDAPLAPKAIYDAEIPVLGICYGEQAMAHQLGGKVEGGHHRE
                     FGRAEVTVTEDSPLFEGVWRKGEKYPVWMSHGDRVTRLPQGFHVFGTSPNAPIAIIGD
                     DKRRFYATQFHLEVMHTPHGAALLRNFVRKIAGLKGDWTMRAFKDEAIEKIRRQVGKG
                     RVICGLSGGVDSAVAAVLIHEAIGDQLTCVLVDHGLMRLGEAQKVVTLFRDSYNIPLV
                     HVDASETFLKALAGVDDPEQKRKTIGKLFIDVFEHEAKKIGGAEFLAQGTLYPDVIES
                     VSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRALGRELDLPEAFVGRHP
                     FPGPGLAIRCPGPITREKLDILRLADEIYIEEIRRSGQYDDIWQAFAVLLPVKTVGVM
                     GDGRTYDYVVGLRAVTSTDGMTADFYLFDMTFLGAVATRIINEVKGVNRVVYDVTSKP
                     PGTIEWE"
     gene            complement(3986..>4110)
                     /locus_tag="VFL49_10990"
     CDS             complement(3986..>4110)
                     /locus_tag="VFL49_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine cyclotransferase"
                     /protein_id="HET7912548.1"
                     /translation="QLDMPRGVAVSGLDSNGSDTFFCGGGSSGKIRAVRKPKRA"
BASE COUNT          791 a         1259 c         1302 g          758 t
ORIGIN      
        1 ccgcgcggtg ctcttgctca agccgggcgg ccgtattgcc tacgtgacct gctcggtctt
       61 ggaagaggaa aacggcgtcc aggtgcgcgc tttcctcgca aggcatgctg cgttcaagac
      121 cgtccccccc gatcaggtgg cgcaggcgct gggcgagcga gccttcatgt tctgcaaggc
      181 agccaggcta tcaagcgaag gcattctgat gacaccacga ctaaccgaca cggacggatt
      241 tttcgtcgcg gtcctgcggc ggggcggctg aggttcctgt aaggatcgga aagaaatcgc
      301 taacggaata ccccgattgc gccccattcc cggccaattt tctccgcgat gatcgcgtgt
      361 ttatggaacc tgcgagattc ttcttcgggg ctttccatgc ttgtcgccag gcgcgatccg
      421 ccatttcatg tggacgcgcg cgatgccgtg tccgccaatt ttgaaacggg atcgaagccg
      481 ccaagggtgg tgcgcttcgt gaccgtcatc cagatgatcg gctcgctggt cgccgtgccg
      541 gtcggtatcg gcagcgccta taccttctat agggcgaatt tttccccgga aacgacctgc
      601 cagagcctgc gcagcggcat cattgcaatg ctcgataaag gtgtggatgc ctcgacgcgg
      661 cgcgtcctgg tgcgccgcga cgtcgagacc ttcgaaaaga cctgcgccac ggtagacccg
      721 gaggctaccg cggcgttcaa gaccttgctg gcagcggaaa aatcagcggc gcccattcct
      781 gcagtggtgc ctgcgccaaa agcccagcca agtgaaaccg tatcgaagga accggtccac
      841 aaagccgagc cgcggccaca gccggcgccc aagcaagccg tcacgaatgc gcaaccgcct
      901 gcggcgcaaa gcacccggaa ggagcccgtt tcggatacgc agtggctcga tgcggttcgc
      961 caggcattgg tgacgcatca agagagcgcg cgcccggccg agggtgtgaa ggcacgcccg
     1021 gcggcggcgc cttccattcg tccggcccag cgcgaagtgg agccggctgc gcctgtggtc
     1081 gcgccggcgc cggtggccac gtcaacgcca gccgcgtccg cggccgcgcc agtattgccg
     1141 ccgccggtcg cggtcgttcc gacgcccgca caacagaacg cggtacggac gcaggatccc
     1201 gatcacccgg taccgccgca atcaatcccc gatgctccgg aggttgccgc cgccgagacc
     1261 aaggctgacg aacccggtcg ctcgaagatc ggaaaatgga tttccgcgat tccgttgctt
     1321 ggcccggtca tagacaacgg ccggcactag tttggttgcc agtccgcaac cgtcgtcagg
     1381 tccgtttcga cttgccatcg tcccggcttg tccgcttgac gcccggactg cgcgaggcgg
     1441 acaaggtagt cctcgcgcgg aatttcccgg aatcccatgt cacggcatgt tggcgtcata
     1501 agcttgccgt cgtttaaggc gaagccccag cgcgcgagat gccagttgag cgcgctgaaa
     1561 cccatctttg atgtattcgg ctcacgcgag aattgtgact cggtgacgaa gacgccgccg
     1621 atcgccacgc catagccgcc gccggcaagc tcaccgcgct cgttccagac ttcgaacgaa
     1681 tgggcatagc cagcgtcgaa caagtcggca taggcgcgca tgatccgcgg tgtgatccat
     1741 gtcagatgcc agcggccgtc ccggtgtgcg gcgcaagccg cgatgaccgc ttcgaaatcg
     1801 ctgtcgagcg tgacggtgta attgtgctgg cgcatctgcc ggcgcaggcg ttttgcgata
     1861 tgaagctcat tgaaaaagag aaccgagcgg aagggcggcg accaccactt caacggtgcg
     1921 acatgcgcaa acggatagag gcctcggcgg tacgcagcga gcaaggtcgg ccggcccaga
     1981 tcatgcacaa cgccggcgag gccgggcggg ctggcgaatg cgcgttgcgg atcgggcaat
     2041 gcgtagtcgg gcgccagaag ctcgttgaaa cagagccgca gcacgttcgg caggccgcga
     2101 attcgggcag gacttaacgc ccaggcgagg ccgaggatgt ggcgttgcgc cacgccggcg
     2161 aggttttcgc gaaacagcgc cgcacggcgg tcggcgcggc cgcgcgtcgc ggccggcaat
     2221 gtcatgcgct cggtttcgaa aaccatggcc gttgcgatgt cttccggctg tgcacaaata
     2281 tcgccgggag gctcttctaa gcatgttcgg cgctaacatt gggttacccc gatcgtagag
     2341 aagcttgacc ttggggaaca aagcccgcaa aaagccgccc cgagcggcct ttgcgcaccc
     2401 gagtcggcca atttcggggc acatcgaccg tctatgcacg acaaggttct gatcgtcgat
     2461 ttcggttccc aggtcaccca gctcattgca cgccgggtgc gggaagaaaa agtctattcg
     2521 gaaatcgttc cctatcagaa agccgagacc gccttccgcg agatgaagcc gaaggcggtc
     2581 attctgtccg gcggccctgc ctccgttctc gacaaggatg cgccgcttgc acccaaggcc
     2641 atctatgacg cggaaattcc tgtgctcggg atttgctacg gcgagcaggc gatggcgcac
     2701 cagcttggtg gaaaggtgga aggcggccac catcgcgagt tcggccgagc cgaggtgacc
     2761 gtcaccgagg attcgcctct attcgaaggt gtctggcgca aaggcgagaa atatccggtg
     2821 tggatgagtc atggcgaccg ggtgaccagg ttgccgcagg gcttccatgt tttcggcacg
     2881 tcgccgaatg cgccgatcgc gatcatcggc gacgacaagc gcaggttcta cgccacgcaa
     2941 tttcatcttg aagtgatgca cacgccgcat ggtgctgcgc tcctgcgcaa ttttgtccga
     3001 aagatcgccg gtctcaaggg cgactggacg atgcgcgcct tcaaggacga agcgattgaa
     3061 aaaatccgca ggcaggtcgg caagggacgg gtgatctgtg gcctgtcagg cggggtcgat
     3121 tccgcggttg cagccgtgct gattcacgag gcgatcggcg accaattgac atgtgttctt
     3181 gtcgatcacg gcctgatgcg gctcggcgag gcgcagaagg tcgtgacgct cttccgcgac
     3241 agctacaaca ttccgctggt gcacgtggac gcctcggaaa cctttctcaa ggcgcttgcc
     3301 ggcgtcgatg accccgaaca gaagcgcaag accatcggca agctcttcat tgacgttttc
     3361 gagcacgagg caaagaaaat cggcggtgcc gaatttctcg cacaaggcac actctatccc
     3421 gacgtgatcg aaagcgtctc cttcaccggc gggccgtcgg tcacgatcaa gtcgcaccac
     3481 aatgtcggcg gcttgcccga gcgcatgaac atgaagctgg tcgagccttt gcgggagctt
     3541 ttcaaggatg aggtgagggc gctcgggcgc gagctcgacc tgccggaagc cttcgtcgga
     3601 cggcatccat tcccggggcc cggccttgcc atccgctgcc cggggccgat tacgcgcgag
     3661 aagctcgaca tcctccggct cgccgacgaa atttacatcg aagagatccg tcgcagcgga
     3721 cagtacgacg acatctggca agccttcgcg gtgctcttgc cggtcaagac tgtgggcgtc
     3781 atgggcgacg gccggaccta cgattatgtc gtcggattac gagcggtgac gtcgaccgac
     3841 ggtatgaccg ccgattttta tctctttgac atgacgtttc tgggcgcggt cgcgacgcgc
     3901 atcatcaacg aagtcaaagg cgtcaatcgc gtcgtttacg atgtgacttc caagccgccg
     3961 ggtacgatcg agtgggagtg aggggttagg ctcgcttcgg ctttcttacc gctctgattt
     4021 tcccgctgct tccgccgccg cagaagaacg tgtcgctgcc gttggaatcg aggcccgata
     4081 ccgccacgcc gcgcggcatg tcgagctgct
//
LOCUS       DASSRL010000540         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1413726, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000540 DASSRL010000000
VERSION     DASSRL010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413726"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFL49_10995"
     CDS             complement(<1..302)
                     /locus_tag="VFL49_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET7912549.1"
                     /translation="MDYVKPADVAAAMVDTGRRKLALSPADLLIRGALAGSILAAATS
                     LALTATVQTGQPLVGALVFPVGLILIVLLGLDLVTGSFGLLPLPWIERDASGTSMLA"
     gene            complement(691..>2229)
                     /locus_tag="VFL49_11000"
     CDS             complement(691..>2229)
                     /locus_tag="VFL49_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HET7912550.1"
                     /translation="RTRKKMEKAFAFMIRVRIPGGVLTPQQWLALDKVARDYGNGTMR
                     MTTRQTVQLHGVIKSNLKATLKSIDTVLLNSIAACGDINRNVMCNVLPEQSRAHAAAI
                     ALARAISDHLLPHTPAYREIWLDGERIAGGEEEAIEPIYGKTYLPRKFKVVVAVPPSN
                     DVDVFAHDLGYIAITDAAGNIAGWNVTVGGGMGMTHGEPDTYPRIAEVMGFCRTDDVV
                     KVSEAVVTVQRDWGDRRNRKHARLKYTIEDRGLDAFRAEVEKRAGIKLEPAKPYTFTS
                     TGDRYGWTEGMDGRGHLTLFVQNGRVHDLAGAAQQSALRSIAQMHDGEFRITPNQNLI
                     ISSVPTERQAEIERIAREAGLLAPWSGLRRNSMACVALPTCGLALAESERYLPELMDA
                     LDEKLAVNGLSADDIVIRMTGCPNGCARPYLSEIGLVGKGPGRYNLYLGAAFDGSRMN
                     KLYAEDLDHAGIVAALDPLFAAYARERQKGERFGDFVIRTGHIAASGNGADFHANVRA
                     YRAK"
BASE COUNT          372 a          732 c          707 g          418 t
ORIGIN      
        1 gccagcatgc tcgtgccact ggcgtcgcgc tcgatccatg gcaagggcag caggccgaag
       61 cttcccgtca ccaggtcgag ccccaacaac acgatgagga tcagcccgac cggaaagacg
      121 agcgcgccga ccagcggctg ccctgtttgc accgtcgcgg tcaacgcgag gctcgtcgcc
      181 gccgcgagaa tgcttccggc aagcgcgccg cgtatcagga gatcggctgg cgaaagcgcc
      241 agcttgcggc gcccggtgtc gaccatcgcg gctgcaacgt ccgccggctt cacgtaatcc
      301 attccaagac tccctttcct gtcggctgcc gcatcccggg caaattttgt cagccagtgc
      361 gaaatctatg ctgcacagca gcaaggaccg tgccagttgg gaggtatctg aacttgtttg
      421 gttatttcga tcctgccgaa tcggccatgc aaaggtgctg cccattgcgc cgccaacggt
      481 cgaatttttg ggcagcggcg ctgtcgttgt catagtgcgc aacgaacagg cgggcgggct
      541 ctcattcgtc atggccggac aaccgggtcc ggccgaaggc cggcccggtc gtaaacttgt
      601 cccgggcatc cacgtttttg cagcctagtc attaagacgt ggatggccgg aacaagtccg
      661 gccatgacga acgagagttc gtgctctccg ttatttcgct ctgtacgccc gcacattcgc
      721 atggaaatca gcgccgttgc cactcgcggc aatgtggccg gtgcggatga cgaagtcgcc
      781 gaagcgctcg cccttctgcc gctccctggc gtaggccgcg aacagcgggt cgagcgcggc
      841 aacgatgccg gcgtgatcga gatcttcggc gtagagcttg ttcatgcgcg agccgtcaaa
      901 ggccgcgccg agatagaggt tgtagcggcc gggtcctttg ccgaccaggc cgatctcgga
      961 aaggtaaggt cgcgcgcagc cgttcgggca tcccgtcatg cgaatgacaa tgtcgtcggc
     1021 cgacaacccg ttcactgcga gcttctcatc gagcgcgtcc atcaactccg gcagatagcg
     1081 ttcgctttcg gccagcgcga gcccgcaggt gggcagcgcg acgcaggcca tcgagttgcg
     1141 ccgcagccca gaccagggcg cgagcaggcc ggcctcgcgc gcgatgcgct cgatctcggc
     1201 ctgcctttcg gtcggcacgc tggaaatgat caggttctgg ttcggcgtga tgcggaactc
     1261 gccgtcatgc atctgcgcaa tgctgcgcag tgcgctctgc tgcgcggcgc cggccaagtc
     1321 gtgaacgcgc ccgttctgca cgaacaacgt gagatgcccg cgcccgtcca tgccttcggt
     1381 ccagccatag cggtcgccgg tcgaggtgaa tgtgtagggc ttcgccggtt cgagcttgat
     1441 acccgcacgc ttctcaacct cggcgcggaa ggcatcgagc ccgcggtcct cgatcgtgta
     1501 tttgagccgc gcatgcttgc ggttcctgcg gtcgccccag tcgcgctgca cggtgaccac
     1561 cgcctcggag actttcacca cgtcgtccgt gcggcagaag cccatgactt cggcgatgcg
     1621 cggataggtg tcgggctcgc catgcgtcat gcccatgccg ccgccgacgg tcacgttcca
     1681 gccggcaatg ttgccggcag catccgtgat cgcgatgtag ccgagatcgt gcgcgaagac
     1741 gtcaacgtcg ttcgagggcg gcaccgccac cacgaccttg aatttgcgcg gcaaataggt
     1801 cttgccgtag atcggctcaa tcgcctcctc ctcgcccccg gcgatacgct cgccgtcgag
     1861 ccagatctcg cgataggccg gcgtgtgcgg cagcagatgg tcggagatcg cgcgcgccag
     1921 cgcgatcgcc gctgcatgcg cgcgcgactg ctccggcagc acgttgcaca tcacgttgcg
     1981 gttgatgtcg ccgcaggccg cgatcgaatt gagcagcacc gtgtcgatcg acttcagggt
     2041 tgccttcagg ttcgacttga tgacgccgtg cagctgcacc gtctgccgag tcgtcatccg
     2101 catcgtgccg ttgccgtagt cgcgcgcgac cttgtcgagc gccagccatt gctgcggcgt
     2161 caacaccccg ccggggatgc gcacgcggat catgaaggcg aaggccttct ccatcttctt
     2221 gcgtgtgcg
//
LOCUS       DASSRL010000541         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1169402, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000541 DASSRL010000000
VERSION     DASSRL010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169402"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..877)
                     /locus_tag="VFL49_11005"
     CDS             complement(74..877)
                     /locus_tag="VFL49_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7912551.1"
                     /translation="MTAITFSDPRLAKLNGFRVELNAEKERADVVLDRPPLNVIEMPQ
                     RDQLRLVFETLDEDDRVRVIVLRAIGEHFSSGGNIKGFMEATPEHVSRLAWNIAAPAR
                     CSKPVIAANRGYCFGVGFEISLACDFRVVSETCSYALPEQKLGQIPGSGGSARLQKIV
                     GITRTKDIVMRSRRIPGRQALDWGIATECVADDRLEAATDALVDELRMFSPIAQRTAK
                     KLLNDTEDSTLAIAIELEGHCYSRLRQSDDFREGVEAFHAKRPPAFKGR"
     gene            complement(874..2430)
                     /locus_tag="VFL49_11010"
     CDS             complement(874..2430)
                     /locus_tag="VFL49_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7912552.1"
                     /translation="MLDLGTSFIASIERDPKALAIVDGAARLTYAEWYRRISGVVAGF
                     DELGLRPGDHLLTTAQNRWEAATLHWACQFAGIIITPVNWRATADELDFLIENAEARA
                     VVYEDATAAAVARSKAAQSRPRIALDMAKSGEIAWSVLSERKARDAAPRVGPDAWSVM
                     LYTSGTTARPKGVPRRQNVERASALAHVAQNMYGNGERTLGIMPLYHTMGVRSLLAMS
                     LIGGAFICLPRFDVVRALELIAAEKITNLYLVPTLYHDLVHHAAFAKTDVRSVRKLGF
                     AGAPMTDGLLKELQAAFKPELFVNHYGSSEIYTFTIDRNAPAKPGSAGKAGINQRVRV
                     MKLGASPEQIAAPGEEGEIIAYLAGDESFEGYWRRPESDAKALRDGWYLTGDTGYFDA
                     DGDLFVTGRVDDMIITGGENVSPVEIESCLSLHPAVSEVAVVGLADERWGKIIAAFIK
                     RSTPVNDAELDQFCRKSGLANFKRPRRYIFVNDIPKSPVGKLLRRKLVAGEYETETTG
                     AGSHQNGTAA"
     gene            complement(2423..2932)
                     /locus_tag="VFL49_11015"
     CDS             complement(2423..2932)
                     /locus_tag="VFL49_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7912553.1"
                     /translation="MPRLSADARHRVRFELNGRTVEADAEPRMLLTDFLRHNLGMTGT
                     HVGCEHGVCGACTVEIDGAPARACLTLAVQADGAKVRTVEGLAPAPGRLSVLQEAFRQ
                     HHALQCGFCTAGILMSLDYYLRHHPNPNEAELRDFLGGHLCRCTGYAPILAAALDAAG
                     KLREASAHA"
     gene            complement(2920..>3435)
                     /locus_tag="VFL49_11020"
     CDS             complement(2920..>3435)
                     /locus_tag="VFL49_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET7912554.1"
                     /translation="QTRSRGTVCGSVAHADPSAEIPLALLALKGAVCLSSQKKRRRVA
                     AEDFFTGLMSTARNDDELIEAVEIPCRTAGHGYGFREFARRHGDFAIVACAAIARKDG
                     VRLAIGGVADVPAARDFPDITGNALDEALDEFAGELEPRDDLHATADYRRALVRRLGG
                     ATIEEARRCRA"
BASE COUNT          609 a         1098 c         1103 g          625 t
ORIGIN      
        1 gcctggtcgc cagcagtcgt tgctctgatc tttggttcgg cgaccatcgg ctctcagtgc
       61 caaacaggcg cggttacctg cccttgaatg cgggcggcct tttggcgtgg aacgcttcga
      121 ctccctcacg gaaatcgtct gactgtcgca gccggctgta acagtgtcct tcaagctcga
      181 tcgcgatcgc aagcgtcgaa tcctcggtgt cgttgagcag cttttttgcc gtgcgctggg
      241 caataggcga gaacatcctg agttcgtcga ccagtgcatc cgttgcagcc tcaagcctgt
      301 cgtcggccac gcattccgtc gcaatgcccc agtcaagtgc ctgcctgccg ggaatacgcc
      361 gcgatcgcat cacaatgtcc ttggtccgcg tgatgccgac gattttctgc aaacgcgccg
      421 agcccccgga gcccgggatt tgccccagct tctgctcagg caaggcgtag gagcaggtct
      481 ccgacacgac gcggaaatcg caggcaagcg aaatctcgaa gccgacgccg aagcaataac
      541 cacggttggc ggctatcacc ggtttcgagc agcgcgccgg cgcggcaatg ttccacgcga
      601 gcctggagac atgctccggc gtcgcctcca tgaagccctt gatattgccg ccgctggaga
      661 aatgttcacc gatcgcgcgt agcacgatca cccgcacgcg gtcatcttca tcgagcgtct
      721 cgaacaccag ccgcagctga tcgcgctgcg gcatttcgat cacgttgagc ggcgggcggt
      781 cgaggacgac gtcggcgcgc tctttctcgg cattcaattc cacgcggaag ccattgagct
      841 tcgccaggcg cggatcggaa aatgttatcg cggtcatgct gcggttccat tctggtgcga
      901 gccggcgccc gttgtctcgg tttcgtattc acccgccacc agttttcggc ggaggagctt
      961 gccgaccggc gactttggaa tgtcgttgac gaagatgtag cggcgcgggc gcttgaaatt
     1021 ggccagtccc gatttacggc agaactggtc gagttcggcg tcgttgaccg gtgtgctgcg
     1081 cttgataaac gcggcgatga tcttgcccca gcgctcgtcg gcaagcccca cgaccgccac
     1141 ttcggacacc gccgggtgca gcgacaggca gctctcgatc tcgaccggcg agacattctc
     1201 gccgccggtg atgatcatgt cgtcgacgcg cccggtgacg aacaaatcgc cgtccgcatc
     1261 gaaatagccg gtgtcgccgg tcaggtacca gccgtcgcgc agcgccttgg cgtcggattc
     1321 cggccggcgc cagtagccct cgaaggattc gtccccggcg agatatgcga tgatctcgcc
     1381 ttcctcgccc ggagccgcaa tctgctccgg cgatgcgccg agcttcatca cgcggacgcg
     1441 ctggttgatc ccggccttgc cggcggagcc cggcttggcc ggcgcgttcc ggtcgatggt
     1501 aaaggtatag atctccgagg aaccatagtg attgacgaaa agctccggtt tgaacgccgc
     1561 ctgcaattcc ttgagcaagc cgtcggtcat cggcgcgccg gcaaagccga gcttgcgcac
     1621 ggagcgcacg tcggtcttcg cgaacgccgc atgatgcacg agatcgtggt aaagcgttgg
     1681 cacgaggtaa agattggtta tcttctcggc tgcgatcaac tcgagcgcgc gaacgacgtc
     1741 gaagcgcggc aggcagatga atgcaccgcc gatcaacgac atcgcgagca gcgagcgaac
     1801 gcccatggtg tggtatagcg gcatgatgcc aagcgtgcgt tcgccgttgc cgtacatgtt
     1861 ctgcgcgacg tgcgcgagtg cggaagcgcg ctcgacgttt tgtcggcgcg gcacgccctt
     1921 cggccgcgcc gtggtacccg acgtatagag catcaccgac caggcatccg gccccacgcg
     1981 gggcgccgca tcgcgcgcct tgcgctcgga cagcacgctc catgcgattt cgccggactt
     2041 tgccatatcc agagcaatgc gcgggcgcga ttgcgcggct ttcgatcgcg cgaccgccgc
     2101 ggcggtcgcg tcctcataga cgacggccct ggcttccgcg ttttcgatca aaaaatcgag
     2161 ctcgtcggcg gtcgcgcgcc agttcaccgg cgtgatgatg atccctgcga actgacacgc
     2221 ccagtgaagt gtcgcggctt cccagcgatt ttgcgccgtt gtgagcagat gatcgccggg
     2281 cctgaggccg agttcatcga accctgcgac cacgcccgaa atgcggcgat accattccgc
     2341 ataggtcagc cgtgcggcgc cgtcgacgat ggcaagcgct ttggggtcgc gctcgatgct
     2401 ggcgataaaa ctggtgccga gatcaagcat gcgcagaggc ctcacgaagt tttccggcgg
     2461 catcgagtgc tgccgccagg attggagcgt agccggtgca gcggcacaga tgtccgccga
     2521 gaaaatcgcg caattcggcc tcgttcggat tgggatgatg ccgcagataa taatcgagcg
     2581 acatcaggat gcccgcggtg caaaagccgc attgcagcgc atgatgttgc cgaaaggcct
     2641 cctgcagcac cgacaaccgg cccggtgcgg gcgccaggcc ctccacggtg cggactttgg
     2701 cgccgtcggc ctgaaccgcc agcgtgaggc aggcgcgtgc cggcgcgccg tcgatctcga
     2761 cggtgcaggc gccgcagacg ccgtgctcgc agccgacatg cgtaccggtc atgccgagat
     2821 tgtggcgcag gaagtcggtg agcagcatcc gcggctcggc gtcagcctcg accgtgcggc
     2881 cgttgagctc gaaccggaca cggtggcgcg catcggcact caggcgcggc atcggcgcgc
     2941 ctcctcgatg gtggcgccgc cgagccgccg caccagcgca cggcggtagt cggcggtggc
     3001 atgcaaatcg tcgcgtggct cgagctcgcc ggcaaattca tcaagcgcct cgtcaagcgc
     3061 attgccagtg atatccggaa agtcgcgtgc agccgggaca tcggcgacgc cgccgatcgc
     3121 gagccgcacg ccgtctttgc gtgcgatggc ggcacaagcg acgatagcga aatctccgtg
     3181 gcggcgggca aattcgcgga acccgtagcc gtggccggcg gtgcggcatg gaatttccac
     3241 cgcttcaatc aactcgtcat cattgcgcgc ggtcgacatc aggccggtga agaaatcctc
     3301 cgccgcaacg cgccgccgtt tcttttgcga tgaaagacag acagcgccct tgagcgcaag
     3361 cagtgccagc gggatttcgg cgctcggatc ggcgtgagca accgagccgc agactgtgcc
     3421 gcggctgcgg gtctg
//
LOCUS       DASSRL010000542         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1483981, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000542 DASSRL010000000
VERSION     DASSRL010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483981"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..766)
                     /locus_tag="VFL49_11025"
     CDS             complement(65..766)
                     /locus_tag="VFL49_11025"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HET7912555.1"
                     /translation="MSRRLVWSLLLGVALSLAALIASSGQDAITTLLRHDIGSLAIKI
                     LLVVFVGGLVLVVFRERLSKAIEAALFWAVIGLLLVVGYSYRFELRDVADRVIAELVP
                     GHVAGHGRNVEVVRGHGGDFSVAAHVNGTRIPMVLDTGATSVVLTQEAAKAAGLPIEV
                     LNYTVNIDTANGRTRAAAVTLDRVAVGGLTERAVPALVVHSGHLKNNLLGMSFLNRLQ
                     SWEVRGDRLRMRGAP"
     gene            complement(771..968)
                     /locus_tag="VFL49_11030"
     CDS             complement(771..968)
                     /locus_tag="VFL49_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HET7912556.1"
                     /translation="MIETPCVKICTLDARQGFCLGCGRTVDEIARWGSMSAAERGHIM
                     DELPKRHAAQAAARTMTASVG"
     gene            complement(1053..>2273)
                     /locus_tag="VFL49_11035"
     CDS             complement(1053..>2273)
                     /locus_tag="VFL49_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7912557.1"
                     /translation="FIALDLIYGLAWMFILIHPVGVDDNSGTFMLFVMLLVVAVSSML
                     ASSLPMAVFAATFPVTTAIALDFVLQGRLRDYILSVMALTAQGYFAALTYQLYSSTLA
                     TLQARAEKDALIGELEQAKAISDEARRRAEAANIAKSRFLAQMSHELRTPLNAILGFS
                     EVMKTEVFGAHTVPAYKEYASDIHGSGVHLLGLINEILDLSRIEAGRYELNEESVSLV
                     AVVEDCHHLLKLRATSRNIMIHEMFEPELPPLWADERAIRQICLNLLSNAIKFTPQGG
                     EIWLKIGWTTAGGQYVSVKDTGPGIPEEEIPVVLASFGQGSNSIKSAEQGAGLGLPIA
                     KSLVDLHGGTFTLKSKLRIGTEVIVTFPAERLVAAIAPLPERAPPIASAADADATSQE
                     KKRLGRRPLFRAGT"
BASE COUNT          493 a          675 c          675 g          430 t
ORIGIN      
        1 aagcgactga tttgggaatc ccaaatcgat tcaacctgaa ctcatcccgc tttagccggc
       61 ccgttcaggg cgccccgcgc atacgcagcc tgtcaccgcg cacttcccag ctttgcagcc
      121 ggttgaggaa gctcattcca agcaaattgt tcttcaggtg cccggaatgt acgaccagcg
      181 ccggaacggc gcgttccgta aggcccccga ccgcaacgcg atcgagcgtt accgcagcgg
      241 cgcgggtacg accgttggcg gtatctatgt tgaccgtgta attcagcact tcgatcggca
      301 agcccgcggc cttggcggct tcctgcgtca acaccacgga ggtcgcgccg gtatccagga
      361 ccattggaat tcgcgtgcca ttgacatggg cggcgaccga gaaatccccg ccgtggccac
      421 gtacgacctc gacattgcgg ccatgccccg ccacatgacc ggggacgagt tcggcgatga
      481 cccgatcggc aacgtcgcgc aattcgaagc gatagctgta gccaaccacg agcagcagcc
      541 cgatgacggc ccaaaaaagc gctgcctcaa tggcctttga taatctttca cggaacacca
      601 ccagcacaag tccgccgacg aagacgacga gcaggatttt gatggcgagt gacccgatgt
      661 cgtgacgaag aagggtagtg atggcatcct gaccgctgct ggcgatcaga gcggcaagcg
      721 acagcgcgac acccagcaga agggaccaga caagacggcg gctcacggaa tcatcccact
      781 gaagccgtca tcgttctcgc agcggcctga gctgcatggc gtttcggcaa ttcgtccata
      841 atgtggccgc gctccgctgc gctcatgctg ccccagcggg cgatttcgtc gacggtgcgt
      901 ccgcaaccga gacaaaagcc ctgacgagcg tcgagggtgc agattttgac gcagggtgtc
      961 tcgatcattg gcctgtctcg aagccgagtc tggcgtgacc ataagcaccg cacgtgagaa
     1021 cgcaatcgcg cgattgtcgc aaggctgaaa tcctaagtgc cggcgcggaa aagcggccgc
     1081 cggccgaggc gtttcttttc ctgcgaggtg gcatccgcgt cggccgcaga tgcgatcgga
     1141 ggcgcacgtt cgggcaatgg cgctatggcg gcgacaaggc gttcagcggg gaaagtcaca
     1201 atcacttccg tgccgatgcg cagtttagat ttcaacgtaa aggttccgcc atgcaggtcg
     1261 acgaggcttt tggcgattgg caggccgaga ccggcacctt gttcggccga cttgatggaa
     1321 ttcgagcctt ggccgaatga cgcaaggaca accggaattt cttcctcggg aatccccggg
     1381 cccgtatcct tgacgctgac gtactgaccg ccggcggtcg tccagccgat cttcagccag
     1441 atctcgccgc cctgtggcgt gaacttgatc gcgttcgaca ggagattcag acagatctgg
     1501 cggatcgcgc gttcatcggc ccatagcggc ggcagttcgg gctcaaacat ctcgtgaatc
     1561 attatattgc ggctggttgc ccgcaacttc agcaagtgat gacagtcttc cacgactgca
     1621 acaagagaaa ctgactcctc gttgagttcg tagcggccgg cctcgattct cgacagatcg
     1681 agaatttcat tgatgagtcc gagaaggtgc acgccggaac cgtgtatatc agatgcatat
     1741 tccttgtagg ccggaacggt gtgtgcgccg aagacctcgg tcttcatgac ctcggaaaat
     1801 ccaagtatgg cgttaagcgg cgtacgcagc tcgtgactca tctgcgccag gaaccgtgac
     1861 ttcgcgatgt tggcggcttc tgcgcgtcga cgcgcttcgt ccgagatcgc ctttgcctgc
     1921 tccagctcgc cgatcagggc atctttttcg gcgcgcgcct gcagtgttgc caaggtgctc
     1981 gaataaagct gatacgtgag cgcggcgaaa tagccctgcg ccgtgagtgc catcaccgaa
     2041 agaatgtagt cacgcaacct gccttgcaga acaaagtcga gcgcgatggc cgtggtaacc
     2101 ggaaacgttg ccgcaaacac cgccatcggc aggctcgatg cgagcatgct ggacacagcg
     2161 accaccagca acatgacaaa gagcatgaat gtgccggagt tgtcgtcgac gccgaccgga
     2221 tgaatcagga taaacatcca ggccaagccg tatatgagat cgagagcgat gaa
//
LOCUS       DASSRL010000543         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_678826, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000543 DASSRL010000000
VERSION     DASSRL010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678826"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFL49_11040"
     CDS             <1..196
                     /locus_tag="VFL49_11040"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7912558.1"
                     /translation="NGDRIRLSVKQRKIELLVDDAELKRRAAQTKPSAPHPERGYARL
                     YAQEILGADQGCDFAFLKPD"
     gene            372..2663
                     /locus_tag="VFL49_11045"
     CDS             372..2663
                     /locus_tag="VFL49_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7912559.1"
                     /translation="MFRLLGVFTAYRDWRLIAFAGLTGAIAGLAAMGFIFFYTGIAST
                     SFTQAVLIAAGTLALLASALVWQIRRSGGEQYLRLDEALNNMTHGLCMFDAQNRLVVW
                     NERYRDMYRIDPRRIWRGCTVRDLLEARIASGTFPLDPQRYDSDLHEALKQGRAFTLN
                     IQLKDGRTIAVVNQPMQSGGWVATHEDITERDRAERELESTRSFLDRIVENVPSPIIV
                     KSIPALRYLLINRAAEKYLGVSRSVMLGKTAADIMPQASAERIETEDRKLVASGEPAF
                     LDEHAIATPANGTRIVTATRLSVTGPDGTPQYLIAVINDLTERKRSEQRIEHMAHHDA
                     LTELPNRSAFNDCIASTIKRTAASGENFALLSVDLDRFKSVNDVFGHEVGDGLLREVA
                     RRLEAVCQGAFLARLGGDEFAVITPTGTKPASAEALAERMVAALDSDIDIDGHTLRIG
                     LTVGIGIYPQDGIDVPTLVANTDAALFRAKSEMRGSIRFFEMSMDKQLREKRALQQDL
                     RSAISRDELDLHYQPQAHIDGEVTGFEALVRWHHPRYGLVPPSTFVPLAEESGLIAPL
                     GEWILRTACREAASWPRPLRIGINLSPVQFRHGDLPRLVHQILLETGLAPSRLELEIT
                     EGVLIDNFTRAVAVLRRLKGLGVRIAMDDFGTGYSSLSYLQSFPFDKMKIDQAFVANL
                     GHSHQATTIIRAVIALGRGLNLPVVAEGVETDAQLRFLAAEGCSEFQGYLVGRPEPID
                     EYAYAVGRAQAGKPRSKALAAAG"
     gene            2834..>3441
                     /locus_tag="VFL49_11050"
     CDS             2834..>3441
                     /locus_tag="VFL49_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HET7912560.1"
                     /translation="MPIIFSVASPLVLAQDQQAQQQPQPPQQALGQQQLQQLVAPIAL
                     YPDALLAQVLTASTYPLEVTLAARWAEKNPNLKGPALEEAMQKEPWDASVKGLTSVPQ
                     VLAMMNEKLEWTTQLGEAFLAQPDDVQSAVQTLRKQAETTGNLKSSKEQKVSRIAAPP
                     NTGYAGPPEYIVIEPVEPDYVYVPVYDPVVVYGAGYWPPAYAP"
BASE COUNT          657 a         1026 c         1072 g          686 t
ORIGIN      
        1 caacggtgac cgcatccggc tgtcggtgaa acagcgcaag atcgaactgc tcgtcgacga
       61 cgccgagctc aagcgccgcg cggcgcagac gaaaccgtcg gcgccgcatc cggagcgggg
      121 ctatgcccgt ctctatgcgc aggaaatcct cggcgccgat cagggctgcg attttgcgtt
      181 cctgaagccc gactgaccgc atttccccgg acttatgccg agaaactatc ggcgcatgtt
      241 cacgcatgcg tagggttaat tgtaattaac cctcgggtgt cccgaaattt aacagtacgc
      301 cagagttgat cgtccagatt atcaaaacat cggcaacttt aagccaatgc gtcctccaat
      361 tggcgcaaga gatgtttcga ttattgggag tttttacggc ctaccgcgac tggcgattga
      421 ttgctttcgc cggcctcacc ggcgctatcg caggtctggc ggcgatgggc tttattttct
      481 tttacaccgg aattgcttcg acctcgttca cgcaggctgt gcttattgcg gcgggtacgc
      541 tcgctttatt ggcttcggca ctggtgtggc agattcgccg tagtggcggc gagcaatatc
      601 tccggcttga tgaagctctc aacaacatga ctcacggcct gtgcatgttc gatgcgcaga
      661 accgcctggt cgtgtggaac gagcgatatc gggacatgta tcgcatcgat ccgcgccgca
      721 tctggcgagg atgcacggtc cgcgatctgc ttgaagcgcg catcgcgtcg gggacatttc
      781 cgctcgatcc gcaacgttat gattcggact tgcatgaggc gctcaagcag ggcagggctt
      841 ttaccctcaa tatccagttg aaagacggcc gcacgatcgc cgtcgtcaac cagccgatgc
      901 aaagcggcgg ctgggtggca acccacgaag acattaccga gcgcgatcgg gccgagcgcg
      961 aactggagag cacgcgatcg ttcctcgaca ggatcgtcga gaatgtgccg tcgccgatca
     1021 tcgtgaagag cattcccgct ttgcgatatc tgctgatcaa ccgtgcggcc gagaagtatt
     1081 tgggagtgag ccgttcggtc atgctcggga agaccgccgc cgacatcatg ccgcaggcga
     1141 gcgcggaaag gatcgagacc gaagatcgca agctggttgc gtcgggcgag ccggcattcc
     1201 ttgatgagca cgcgattgca acgccggcca acggcacgcg aatcgtcacc gccacgcgcc
     1261 tgtcggtgac ggggcctgac gggacgccgc agtatttgat tgccgtgatc aatgacctga
     1321 ccgagcgcaa acggagcgag cagcgcattg agcacatggc acatcacgac gcgctcaccg
     1381 aattgccgaa tcgctctgct ttcaacgact gcatcgcgtc gacgatcaaa cgcacggcag
     1441 cttccggcga gaacttcgcg ctgctttccg tcgatctcga tcgcttcaag tccgtcaacg
     1501 acgtgtttgg gcacgaggtt ggcgacggct tgcttcgtga ggtggcgcgc cggctggaag
     1561 cggtatgcca gggtgctttc ctggcccgac tcggcggcga cgagtttgcc gtcatcacgc
     1621 cgaccggcac caagcctgcg tcggcggagg cgctcgccga gcgaatggtc gcggcgctgg
     1681 attccgacat cgacatcgac ggacacacgc tgcgaatcgg gcttaccgtc ggtatcggga
     1741 tttacccgca ggacgggatc gacgtaccga cattggtcgc caacaccgac gctgcgcttt
     1801 tccgcgccaa gtccgagatg cgcggatcga tccgcttctt tgaaatgtcg atggacaagc
     1861 agttgcgcga gaaacgggca ctgcagcagg acctgcgttc cgcgatttcg cgtgacgagc
     1921 ttgatctgca ctatcagccg caggcccata tcgatgggga ggtgaccggc tttgaggcgc
     1981 tggtgcgctg gcatcatccg cgttacggcc tggtgccgcc gagcaccttc gtgccgcttg
     2041 ccgaagagag tggcctgatc gctcctctcg gcgaatggat tttgcgaacg gcgtgccgcg
     2101 aggcggcgtc gtggccgcgg ccgttgcgca tcggcatcaa tctgtcgccg gtacaatttc
     2161 ggcacggcga tctgccgcgg ctggtgcacc agattctgct ggagacgggc cttgccccct
     2221 cacggctgga gcttgaaatc accgagggcg tcctgatcga taatttcacg cgcgcggtgg
     2281 cggtcctgcg tcggctgaaa ggcctcggcg tgcgtatcgc catggatgac ttcggcaccg
     2341 gctattcgtc gctgtcctat ctgcaatcct tcccgttcga caagatgaag atcgaccagg
     2401 ccttcgttgc caatctcggg cacagccatc aggcgaccac gatcatccgc gcggtcatcg
     2461 ctctgggccg cgggctcaat ttgccggtgg tagcggaagg cgtcgaaacc gacgctcagc
     2521 tccgctttct cgcggctgaa ggctgcagtg agtttcaggg ttatctggtt gggcggcccg
     2581 aaccgatcga cgagtacgca tacgccgtcg gacgcgcgca ggccggtaag ccgcgaagca
     2641 aggctctggc ggcggccggt tgaaactcta tcccccttca aatgggggac ctcgaacgat
     2701 acgatgttct ctcctgggcc cttgggggct gtcgcaagcg acggagggat agcccatgtc
     2761 aacttggtca atgtcagctt ggtcaatgtc agcttggtca atgcgtgcgc gttgctgcat
     2821 cgtcgcgttg gcagtgccga taattttttc agtcgcatcg ccgctagtgc tggcgcagga
     2881 ccaacaggcc caacagcagc ctcagcctcc gcagcaggct cttggccagc agcaactcca
     2941 gcagctcgtt gcaccgattg cgctctatcc cgatgcgctg ctggcgcagg tgctgactgc
     3001 gtccacctat ccgcttgagg tcacgctggc cgcgcgctgg gccgagaaga atccgaatct
     3061 taaaggtcct gctctcgaag aagcgatgca aaaggagccg tgggacgcga gcgtcaaggg
     3121 actgacctcg gtgccgcagg tgcttgcgat gatgaacgag aagctggagt ggacgaccca
     3181 gcttggcgag gccttcctgg cgcaacccga tgacgtgcaa agcgcggttc agacgctgcg
     3241 caagcaggcg gaaaccaccg gcaatctgaa gtcgagcaag gagcagaagg tcagcagaat
     3301 cgccgcgccg ccgaacacag gctacgccgg accgcctgaa tatattgtca tcgagccggt
     3361 tgagccggac tacgtatatg ttccggtcta tgatcctgtg gtcgtatacg gcgccggtta
     3421 ctggccgccg gcctatgcgc c
//
LOCUS       DASSRL010000544         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_504270, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000544 DASSRL010000000
VERSION     DASSRL010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504270"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFL49_11055"
     CDS             <1..365
                     /locus_tag="VFL49_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012753362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7912561.1"
                     /translation="DGIIRFWNPGAERIFGWAAAEAIGSSLDLIIPERLRQRHWDGYR
                     ETMKTGKSRYGDGDILSVPALRQDGSTISIEFTIVLLRDQSGQMPGIAAVMRDVTKRF
                     EEMRMLKRRLAETAKSAG"
     gene            376..912
                     /locus_tag="VFL49_11060"
     CDS             376..912
                     /locus_tag="VFL49_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7912562.1"
                     /translation="MVYPFFTIGHSTRPIDEFMDLLRGSEITFVVDVRTVPRSRTNAQ
                     YNRDVLPQSLASFQVGYDHVAALGGLRGRQRDVSPGVNAFWQNESFHNYADYAMSDSF
                     RAGLDRLRELGETQRCAIMCAEAVWWRCHRRIITDYLLARGDPVFHILGPRHIEPAKL
                     NEAARQQPSGILIYPPAH"
     gene            1040..1495
                     /locus_tag="VFL49_11065"
     CDS             1040..1495
                     /locus_tag="VFL49_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7912563.1"
                     /translation="MQTLTPGVTKANSGLDGIVWSILGQTYVPKQLSENSFAWHATLP
                     PTTFVPPHIHPTQDEFVYMLEGRMDALIDGKEFTATAGDLIKLPMGIPHGLFNKSDQV
                     LKCLFWVTPTRKLYDLFWAIHSMKEQNPPDVVALSAKHEVNFLPPPPGA"
     gene            complement(1492..2742)
                     /locus_tag="VFL49_11070"
     CDS             complement(1492..2742)
                     /locus_tag="VFL49_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HET7912564.1"
                     /translation="MKAIIVGGGIGGLTTALMLHARGIDCEIFEQAEGIRELGVGINT
                     LPHAIKELAQLGLLSKLDQVGIRTHELFYMNRIGQEIWREPRGTDAGFEYPQFSIHRG
                     RLQAVIYQTVRARLGESRIHTGHRLGSFTQDEGGVTGYFFGREGSHRVTARGDILIGA
                     DGIHSIVRETLYPNEGPARWNGSMLWRGATDWPKFLTGRSMVIAGGMAGKLVVYPIGE
                     GAREDKPLTNWAVLVKVGEGGAPPRKEDWSRTGRFEDLMPHVQRFSIPFVDAKALIES
                     TPEFWEYPMCDRDPLPHWSHGRVTLLGDAAHPMYPVGSNGASQAILDARCLADRLYEA
                     DHVGQGLWAYERERLPMTAQIVQSNRSGGPEGVIDAVEQRAPDGFDNIDNVLSYEERK
                     AIVRGYAAKAGFAKEQVNKAEKVA"
     gene            2891..>3534
                     /locus_tag="VFL49_11075"
     CDS             2891..>3534
                     /locus_tag="VFL49_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912565.1"
                     /translation="MRACVSKLAVAAAFVFVVNAAAAQDKIRIGLIYTLSGPASALGQ
                     QSKNGFELALKDLGGKMGGKDVELVVADDELKPDIAIQKVRGLLERDKVDFVVGPIFS
                     NILVAIHKPVIDAGKILISTNAGTSNFAGASCNANFFVTSYQNDQIFEAIGEAANKLG
                     YKRVYALVPNYPAGKDALAGFKRTFKGQIVDESLVPLNTTDFGPEVSKISAAKP"
BASE COUNT          703 a         1043 c         1056 g          732 t
ORIGIN      
        1 gcgatggaat catccgtttc tggaatccag gtgctgaacg catttttggc tgggccgccg
       61 cagaggctat tggttcctcg cttgatctca ttatccccga gcggctaagg cagcgtcatt
      121 gggatggcta ccgggaaacc atgaagaccg gcaaaagccg gtatggcgac ggagacatac
      181 tctccgtacc tgcacttcgg caggacggct caacgatttc aatcgaattc acgattgttc
      241 tgctgcgcga tcagtcgggg caaatgcccg gcatagccgc cgtcatgcgc gatgtgacga
      301 agcgcttcga ggaaatgcgt atgttgaagc gcaggctggc tgaaacggcc aagtcggcag
      361 gctagtccga gtagaattgt gtatccgttc tttacgatag gacattcgac gcgaccgatt
      421 gacgaattta tggatctgtt gcgcggttcc gaaatcacgt ttgtcgtcga cgtccggacc
      481 gtgccgcgtt cccgcacgaa tgcgcaatac aatcgcgatg tgctgccgca atcgctggca
      541 tcatttcaag tcggatatga ccatgtcgcg gcacttggcg gtctgcgtgg acggcagcgc
      601 gacgtgtcac cgggcgtcaa tgcattctgg cagaacgaaa gctttcacaa ctatgccgac
      661 tacgccatga gcgatagctt tcgcgctggt ctcgaccggc tccgcgagct tggcgaaacc
      721 caacgctgtg ccatcatgtg cgccgaagca gtgtggtggc gatgccatcg acgcatcatc
      781 accgactatc tgctggcgag gggcgacccg gtattccaca tcctcgggcc gcggcatatc
      841 gaacccgcga agttgaacga ggcggcgcgg caacagcctt cgggcatcct gatctatccg
      901 cccgcccatt aggggcgcga gccactatcc ggtgctggct gcggtctcct tagtaagggg
      961 acgactgccg ggccgggtgc tggcgcttct ccgaggccta atttaggtta tgcaaagttt
     1021 gcgaaaggga gtggcggcaa tgcagacgtt gacaccgggc gtcaccaagg ccaattcggg
     1081 cttggatggg atcgtttgga gcattctggg gcagacctat gttcccaagc aactctccga
     1141 aaattcgttc gcctggcatg cgactttgcc gccgaccact ttcgtgccgc cgcacattca
     1201 tccgacgcag gacgaattcg tttatatgct ggaagggcgt atggacgctt tgatcgacgg
     1261 caaagagttc acggcaacgg ccggcgacct gatcaagctg ccaatgggca ttccgcacgg
     1321 actgttcaac aagtccgatc aggtcctcaa atgtctgttt tgggtgacgc cgacacgcaa
     1381 gctctacgat ctgttctggg cgattcactc gatgaaagag caaaatccgc cggatgtcgt
     1441 cgcgctctca gccaagcatg aagtcaactt tctgccgccg ccgccgggcg cttaggcgac
     1501 cttttctgct ttgttgacct gctccttcgc gaagcctgcc ttggccgcgt aaccacgcac
     1561 gatcgccttg cgctcctcat aagacagaac gttgtcgata ttgtcgaagc cgtcaggcgc
     1621 gcgttgttcg accgcatcga ttacgccttc cggtccgccg gaccggttcg actgtacgat
     1681 ctgtgcggtc atcggcagcc tttcacgttc gtaagcccat agcccctgac cgacgtgatc
     1741 ggcttcgtac agccggtcgg ccaggcagcg cgcatcgagg atcgcctggg aggcgccgtt
     1801 cgatccgacc ggatacatcg gatgcgcggc atcgccgagc aatgtgacac ggccatgcga
     1861 ccagtgcggt aacggatcgc ggtcgcacat cggatactcc cagaactccg gagtcgattc
     1921 gatcagcgcc ttggcgtcga cgaatggaat actgaagcgt tggacgtggg gcatcagatc
     1981 ttcgaacctg ccggtacgcg accagtcttc cttgcgcggg ggcgcgccgc cttcgccgac
     2041 ctttacgaga actgcccaat tggtgagcgg cttgtcctcg cgggcgcctt cgccgatcgg
     2101 atagacgacg agcttgccgg ccatgccgcc cgcgatcacc atcgagcgtc cggtcaggaa
     2161 cttcggccag tcggtggcgc cgcgccagag catcgagccg ttccagcgcg ccgggccttc
     2221 attcggataa agcgtttcgc gcacgatcga atggattccg tcggcgccga tcagaatgtc
     2281 gccccgcgcg gtcacgcggt gcgagccctc gcggccgaag aaatatccgg tgacgccacc
     2341 ttcgtcctgc gtgaacgagc caagccgatg accggtatga atgcgggatt cacccagtcg
     2401 tgcgcgcacg gtctggtaga tcaccgcctg cagccggcca cgatggatag agaattgagg
     2461 atattcgaag ccggcgtcgg taccgcgcgg ttcgcgccag atctcctggc cgatccggtt
     2521 catgtaaaag agctcgtggg tcctgatccc gacctggtcg agcttcgaga gcagcccgag
     2581 ttgggccagc tccttgatcg catgcggcag cgtgtttatg ccgacgccga gctcgcgaat
     2641 gccttcggcc tgctcgaaaa tctcgcaatc gatcccgcgc gcatgcaaca taagcgccgt
     2701 ggtaagaccg ccgatgccgc cgccgacgat gattgccttc atggcccgcg cccaacgctg
     2761 ataactgcgg gcataatggg ccttgcgcgg cgtcgccgca atcggccgct attgcagcgg
     2821 tgattgactc ccccgcgctt tattgaacca ataggccgga aaagctggcg ttttggggag
     2881 gaattggatc atgcgtgcct gcgtaagcaa attggccgtc gctgcagcat ttgtcttcgt
     2941 agttaacgcc gcggcggctc aggacaagat ccgcattggt ctcatctaca ccctctcagg
     3001 gccggcctcc gccctcggcc agcagtcgaa aaacggtttc gagctggcgc tgaaagattt
     3061 aggcggcaag atgggcggga aagatgtcga gcttgtcgtg gctgacgatg agctcaagcc
     3121 cgatatcgcg attcagaaag tccggggttt gctcgaacgc gacaaggttg actttgtcgt
     3181 tggtccgatc ttttccaaca ttttggtggc gatccacaag ccggtcatcg atgccggaaa
     3241 aattctgatc agcaccaacg ccggaacgtc taatttcgcc ggcgcgagtt gcaatgccaa
     3301 tttcttcgta acgtcgtatc agaacgatca gatattcgag gcaatcggtg aagccgcgaa
     3361 caagctcggt tacaagcggg tctacgcgct ggtgccgaat tatccggccg gcaaggatgc
     3421 gcttgccggg ttcaagcgca ccttcaaagg acagatcgtc gacgagtcgc tggttccgct
     3481 caacacgacc gactttggtc cggaagtgtc aaaaatctcc gcggcgaaac cgga
//
LOCUS       DASSRL010000545         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1081848, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000545 DASSRL010000000
VERSION     DASSRL010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081848"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            653..2326
                     /locus_tag="VFL49_11080"
     CDS             653..2326
                     /locus_tag="VFL49_11080"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET7912566.1"
                     /translation="MLDPASKGKRPDREASGGPPLDFQEHLRRLEAHGLLTRIDRAID
                     KDRELHPLVRWQFQGGLTEEQRRAFLFTNVVDGSGRRYEIPVAVGALGASPEIYALGM
                     GKTVAAIGDAWTQAIANPIAPVTTQSGPCQEVITRGRDLLRPGGGLAQLPVPISTPGF
                     DSAPYLTATLCITRDPETGVQNMGTYRGALKAQDRLGVRMSSRPGGAGGYLHWRKYQK
                     RKQPMPCAIVIGCAPVVLFTGPQKLPVDQDEMAVAGGLAGAPIRIVKAVSVDLNVPAD
                     AEIVIEGLIDSELLEPEGPFGESHGHVALEDFNMSMTVTAITHKRNPVFVSIISQVTP
                     SESSVIKKVAFEPMFLAHLRDQLGIRGIRKLVMHEPFTNLRKVIFLQFAKGTARTEVW
                     RGLHGAASLRADCGKICVAVSEDIDPANTDAIFWSLAYRSDPGTDVHVAPYRAAGHGP
                     KSGAREDSTILIDATLKHDMPPLALPGREYMEHARTIWNELGLPAISPQPPWHGYSLG
                     DWDERYDVYARRAVEGRWAESGEETIKRRRGGLIPETPVRNVEDAKKRS"
     gene            2331..3332
                     /locus_tag="VFL49_11085"
     CDS             2331..3332
                     /locus_tag="VFL49_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912567.1"
                     /translation="MTFLRSVAVLVIALAFAASQDASAQDSYPARPVRLIVGFAAGSS
                     GDVAARIVGHKLGELLGQQIVVENRPGASSMIATDYVARSPKDGYTLLFGTIALTINP
                     ILMPTKAPDVEKDLAPIVLVGSIPNILVVSKDLNVSSVKELIALAKQKPDELLYGAAG
                     LGSGPQMATELFKQMAGIKMTGVLYPGSAQTVMDLVSGRIQVMFSPASTVLGFIKDGR
                     VKALATSEAQRSSAAPDLPTIAEAALPGYATGLWFGILGPAGTPKPIIDKISEAANKA
                     LKDPNVLKQLETQGLSALGGSPDDFARYIKSESERWARILHDMEAAKAGKEPARQAQ"
BASE COUNT          718 a         1076 c         1063 g          654 t
ORIGIN      
        1 gacgtcgaat gcagcgacaa attcgacgtc gctgcagcga tacccgccga tcaaatgacg
       61 cataactcct tcaagctcag caccctgacg atcccggtaa tattctacgc cctgcaccaa
      121 tgaactggcg caattgccga ccccggcaat ggcgactttg acggtcttac gcatgctcgc
      181 tacgtccccg aaaacgcgat tgttggttcg ctagaaaacc tgttataact caaaaggtta
      241 gctagaagcg gcggccccat gcctgtgctg caaactttat actgcatcaa ccacctcacg
      301 gccatctggc tttaggtggc tttttcgtgc gtgaggcttt tgggaaacag tccggcaaag
      361 ccgccagttg cgaccagacc gaggttccaa tttaacggcc gcagcggctg accaggctgt
      421 ctggcgacaa agcacccatt taacccgatg cggagcgcga caagcccaat caattctcgt
      481 tctcgcgcca cgacgcaaaa cccgcgcggc gtttcgtact tgcgctcgca gcgcgcttcg
      541 ccgtcctcat gtcgcacgta gcgaccttgg agtcagcggc aacggattga ttttgcctcg
      601 cggaacacgc tgccatagtc tagcgccgta agaagtcccg caggggtggt gcatgctcga
      661 tcctgccagc aaggggaaaa ggccggatcg agaagcatcc ggcggccctc cgctcgattt
      721 ccaggaacat ctgcggcgac tcgaagcgca cgggcttctc acgcgcattg acagagccat
      781 tgacaaggac cgggagcttc acccgctcgt ccgctggcag tttcagggcg gtctcacgga
      841 ggaacagcgc cgggccttcc tctttacgaa tgtcgtcgat ggttcagggc ggcgctacga
      901 gattccggtg gctgtcggcg cgttgggtgc ctcgcccgaa atttacgcgc tgggaatggg
      961 caagacggtt gcagctatcg gcgatgcctg gacgcaagcc attgccaatc caatagcgcc
     1021 tgtcacgacg caatccggcc cctgtcagga ggtcatcacc cggggccgcg atctgctgcg
     1081 gccgggcggc ggccttgcgc aactgccggt gccgatttcg actcccggtt tcgactccgc
     1141 tccatacctc acggcaacgc tatgcatcac ccgcgatcct gaaaccggcg tccagaacat
     1201 ggggacttac cgcggtgccc tgaaggcgca ggaccggctg ggcgtgcgaa tgtcttcgcg
     1261 tcccggcggc gccggtggct atctgcattg gcgaaaatat caaaagcgca agcagccgat
     1321 gccctgtgcg attgtcatcg gctgcgctcc ggtcgtgctg ttcaccggtc cgcagaagct
     1381 gccggttgac caggacgaaa tggccgttgc gggcggcctc gccggcgccc cgatccgtat
     1441 cgtcaaggcg gttagcgtgg atctgaatgt tcccgctgac gctgaaatcg tcatcgaagg
     1501 cctgatcgac agcgagctgc tcgagcccga aggcccgttt ggagaaagtc acgggcacgt
     1561 ggcgctcgaa gatttcaaca tgtcgatgac ggtgaccgcg atcacgcaca agcgcaatcc
     1621 ggttttcgtc tcgatcatca gccaggtgac gcccagcgaa tcgagcgtga tcaagaaggt
     1681 cgcgttcgag ccaatgttcc ttgcgcattt gcgcgaccag ctcggcatcc gcggcatcag
     1741 gaagctcgtc atgcacgagc cgttcacgaa tctgcgcaag gtcatctttt tgcagttcgc
     1801 gaaagggacc gcgcgaacgg aggtttggcg cggtctgcac ggcgcggcgt cgctgcgtgc
     1861 cgactgcggg aaaatctgcg tcgccgtcag cgaggatatc gatccggcca acaccgatgc
     1921 catcttctgg tcgttggcct accgctcgga ccccggcacc gacgtgcatg tcgcgcctta
     1981 ccgcgccgcc ggacacgggc caaaatccgg ggcgcgggaa gattccacca ttctgatcga
     2041 cgcgacgctc aagcacgaca tgccgccgct cgctttgccc gggcgcgaat acatggagca
     2101 tgccaggacg atctggaacg aactgggctt accggcaatt tccccgcagc cgccatggca
     2161 cggctattcg ctcggggact gggatgaacg gtacgacgtt tacgcccggc gcgcggtcga
     2221 aggacggtgg gcggaaagcg gagaggaaac gatcaagcgc cggcggggcg gcctcatacc
     2281 cgagactccg gtgcgcaacg tcgaagatgc caagaagaga tcctgagccc atgacctttt
     2341 tgagatctgt cgccgttctc gtcattgcgc tggcgtttgc cgcatcacag gacgcatccg
     2401 cgcaggatag ctatccggct cgcccggtcc ggttgatcgt cggatttgcg gcaggatcga
     2461 gtggcgacgt cgcggcgcgc atcgtcggcc acaagctcgg cgaattgctt ggacagcaaa
     2521 ttgtcgtcga aaatcgaccc ggcgccagca gcatgatcgc aaccgattac gtcgcgcgca
     2581 gtcccaagga cggttacacg ctgctgttcg gcacaatcgc gctgacgatc aatccgattc
     2641 tgatgccgac caaggcgcca gacgttgaaa aggatctggc gccgatcgtg cttgtaggct
     2701 cgatccccaa cattctggtg gtcagcaaag atctcaatgt ctcgagcgtg aaagagctca
     2761 tagcgctcgc caagcagaag ccggacgagc tgctttacgg ggcagcgggc ctgggtagcg
     2821 gaccgcaaat ggcaaccgag ctgttcaaac agatggcggg catcaagatg accggggttc
     2881 tgtatccggg cagcgcgcag acggtcatgg atctggtatc cggccgcatc caggtgatgt
     2941 tttcccccgc ctcgacggtg ctcggattta tcaaggatgg acgggtgaag gccctggcga
     3001 ccagcgaagc ccagcgcagc agtgctgcgc ccgatctgcc gacgatcgcg gaagcagcac
     3061 tgcccggtta tgcgacgggg ctttggttcg gcattctcgg tcccgccggt acgccgaagc
     3121 cgattatcga caagatttcc gaagcagcca acaaggccct gaaagaccca aacgtgctca
     3181 agcagctgga gacgcaaggc ttgagcgcgc tgggcggcag ccccgacgat ttcgcccgct
     3241 atataaaaag cgagtcggaa cgctgggcgc gaatcctgca cgacatggag gccgcgaaag
     3301 cgggcaagga gcccgcgcga caggcgcagt aagtatatca actatgattt ttgattcctt
     3361 gccgcagcga cgagtaccgg cgcgcccaaa ccggacgctt caccggatat cattgcaaga
     3421 tattcgctga cattgtgagc cactgcatcg cgctccacgc gggacgcaat gctttgtgca
     3481 taagcacata gcatcgcctg ttcgacctgt t
//
LOCUS       DASSRL010000546         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1571850, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000546 DASSRL010000000
VERSION     DASSRL010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571850"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFL49_11090"
     CDS             complement(<1..742)
                     /locus_tag="VFL49_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HET7912568.1"
                     /translation="MVRVLRAVARFFQETIGWNRIGVMLSVTIISVAVVVLYHMLQDI
                     NVDEVVAALKATDPTHIAMAALFVACGYFTLTFYDLFALRTIGRKEIPYRIAALTGFT
                     SYSIGHNIGATVFTGGAVRYRIYSAYGLDAIEVAKICFVAGLTFWLGNATVLGLGVTY
                     HPQAAGPIDQLPVWLNRVIGIAILLVLISYVAWVWSAPREIGQREWKVELPSGPSTLL
                     QIGIGILDLGSCALAMYMLLPAHPDIGFV"
     gene            921..1934
                     /locus_tag="VFL49_11095"
     CDS             921..1934
                     /locus_tag="VFL49_11095"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7912569.1"
                     /translation="MSRSDQSRRTVLKSACLGVGAGLLSGLTASAEATENEEIWAHEY
                     WANKDGVKLNLWRKRAGAPSPGEPTRPVLFLVHGSSNSSRSSYDLTVPGKGEYSLMNV
                     FARYGYDVWTMDHDGYGYSGSSGNNSDIASGVEDIKAAIPVVMKEAGRQKAHFYGTSS
                     GGIRAAAYAQAQPEAVDRLLLCAFTYKGNGAAEIERRRKRIDALRASPRRKRDAEMIR
                     SIFTRDGHPSLYDPAVPEAIIAAEMKFGDTIPSGTYLDMAANLPVVDPTKIRSPVLML
                     RGEFDGNSTNDDLLDFYRQLPNGDRQFVILPQTAHSAGYSSNRHLLWYAMKNFLAAPS
                     PTA"
     gene            complement(1935..>2384)
                     /locus_tag="VFL49_11100"
     CDS             complement(1935..>2384)
                     /locus_tag="VFL49_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7912570.1"
                     /translation="WHWFFNIQPYDLPERMMGADPDWFIEKKLAKTRQGLSFFDPAAL
                     AEYKRCFRNPHTIHAICEDYRATFGVDLEMDTKDFDAGRKIECPVLLLWGATGGVGRN
                     HKPGPEEIWRDYAANIVGAKALPCGHYLSEEAPKETTEALRAFFTAA"
BASE COUNT          504 a          742 c          697 g          441 t
ORIGIN      
        1 tgacgaagcc gatatccgga tgggccggca gcagcatgta catggcgagc gcacagctgc
       61 cgagatcgag aataccgata ccgatctgca gcaaggtgga cggcccgctc ggcaactcga
      121 ccttccattc acgctggccg atttcccgcg gagcgctcca gacccaggcg acgtaggaga
      181 tcaataccag caatatcgcg atgccgatca cgcgattgag ccacaccggc agctgatcga
      241 tcggtcccgc cgcctgcggg tgatacgtga cgccgagtcc gaggaccgtg gcgttaccga
      301 gccagaacgt caggccggcg acgaaacaga tctttgcaac ttcgatggca tccagcccat
      361 aggccgaata aatgcggtag cgaacagcgc cgcccgtgaa aaccgtagcg ccgatgttgt
      421 gaccgatcga atagctcgtg aaacccgtca gcgcagctat gcgataagga atttccttgc
      481 gaccgatcgt gcgcagggcg aacaggtcgt agaaagtgag cgtgaaatag ccgcacgcta
      541 caaacaacgc ggccatcgcg atatgagtcg gatctgtcgc cttgagtgcc gctaccacct
      601 cgtcgacatt gatgtcctgc agcatgtggt agagaacgac gactgccacc gagatgatgg
      661 tgacgctcag catcacgcca atccggttcc agccgatcgt ctcttggaaa aagcgcgcca
      721 ccgcgcgcaa tactcggacc attattcctc cgcttcggcg tatcgccccg aacgccgcgc
      781 gccgcgatag cgctgtcccg acccctagca gaactcagat cggggggcga gtagactgcc
      841 ttctcaccca tgtcatgtga taggatgccc aaaatatggc cattaataat tgtcggccgc
      901 agtcttttcg ggagatcccg gtgagccgat ccgaccagtc ccgccgcacg gtcctcaaga
      961 gcgcctgcct tggcgtgggc gccgggcttc tgtctggcct cactgcgtca gcagaggcga
     1021 cggaaaacga ggaaatatgg gcccatgaat attgggcgaa caaagacggc gtgaaactta
     1081 acctgtggcg caagcgcgcc ggtgcaccct cacccggcga accgacgcgc ccggtcttgt
     1141 ttcttgtcca cggctcatca aattcatcgc gttcgtccta cgatctgacc gtgccgggga
     1201 aaggcgaata ttcgctgatg aacgtctttg cccgttatgg ctacgacgta tggacaatgg
     1261 atcatgacgg ttacggctat tccggctcat ccggcaacaa ttccgacatc gccagcggcg
     1321 ttgaggatat caaagccgca atcccggtcg tgatgaagga agccggccgg cagaaggcgc
     1381 atttctacgg cacgtcgtcg ggtggaatcc gcgccgctgc ttacgcgcag gcccagcctg
     1441 aggcggtcga ccggctgctg ctttgcgcct tcacctacaa aggaaacggc gccgccgaga
     1501 tcgagcgccg cagaaagaga atcgacgcat tgcgcgccag cccgcgtcgc aagcgcgacg
     1561 cggagatgat tcggtcgatc ttcacgcgcg acgggcaccc ctccctctat gatccggccg
     1621 tgcccgaggc gatcatcgcg gcggaaatga agttcggtga cacgattccg agcggcacct
     1681 atctcgacat ggccgcgaac ctgccggtgg tcgatccgac gaagatcagg tcgcccgtgc
     1741 tcatgctgcg cggagagttt gacggcaatt caaccaacga cgacctgctc gacttctacc
     1801 gccagcttcc gaatggagac cggcagttcg tcatcctgcc gcagacggcg cacagcgccg
     1861 gttacagcag caaccggcat cttctctggt atgcgatgaa gaattttctt gctgcgccct
     1921 ctccgaccgc atgatcaggc cgctgtgaaa aaggcgcgca gcgcctcagt ggtctccttc
     1981 ggcgcctctt ccgagaggta atgcccgcaa ggcagcgcct tggcgccgac gatgttggca
     2041 gcgtagtcgc gccagatttc ttccggaccc ggcttgtggt tccgtccgac gccgccggtc
     2101 gccccccata gcaacaggac cggacactcg atctttcgcc cggcgtcgaa gtcctttgtg
     2161 tccatctcga gatcgacgcc aaaagtagcg cgataatcct cgcaaatggc gtgaatggtg
     2221 tgcggattgc ggaagcagcg cttgtattcg gcaagcgccg ccggatcgaa gaaactcaac
     2281 ccctgcctgg ttttcgcgag ctttttctcg atgaaccagt ccggatcggc gcccatcatg
     2341 cgctcgggca aatcgtaggg ctggatattg aagaaccagt gcca
//
LOCUS       DASSRL010000547         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_382071, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000547 DASSRL010000000
VERSION     DASSRL010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382071"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFL49_11105"
     CDS             <1..507
                     /locus_tag="VFL49_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7912571.1"
                     /translation="KEKMPPHWVGLKFFNVYGPNEYHKGHMASVLAKVFDLAKSGQPV
                     KLFKSHRQGIADGDQRRDFIYVDDAVSVLRWLLEASGINGIFNVGTGKAESFRDMIGA
                     MFRSLRMPANIEYIDMPQAIRGQYQYFTEARVERLRRAGYNAGFTPLEAAVGQYVTGY
                     LDRDDRYR"
     gene            512..1990
                     /gene="rfaE1"
                     /locus_tag="VFL49_11110"
     CDS             512..1990
                     /gene="rfaE1"
                     /locus_tag="VFL49_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET7912572.1"
                     /translation="MYDFDKHLSALGRQTILCVGDLMLDEFVYGDVSRISPEAPTPVI
                     AVKRTDLMIGGAGNVARNLVALGARCIFVGAVGDDEAGGALTKALGTHPLIEFHLVID
                     GKRQTTRKVRFVSEHHSTHLLRADWETPSAVDAGSEDALIGHVIKAMARVGAVVLSDY
                     AKGALTPRVIRAVIDAANERGKPVVVDPKGSDYGIYRGATLITPNRQELADATRRPAG
                     TDDEVAAAANELRDALGTEAVLVTRSEAGMTLVGDGAPVHVPAYPVRVRDVSGAGDTV
                     VAVVSAMLAMQADFEAAMRAANAAAAVVVGKRGTATASVAELRSRILPAATLAPEEKI
                     VFDWGVLDDHLSDWRRQGLRVGFTNGCFDLLHPGHVKLLAGARAACDRLVVGLNGDAS
                     VTRLKGAGRPVQPVQARAEVLAALEAVDLVAVFDEDTPEKLIAHVKPTVLVKGADYTR
                     EQVVGREIVEALGGEIILIDLVPGHSTSSMVERTRAPKSRSA"
     gene            2003..>2534
                     /locus_tag="VFL49_11115"
     CDS             2003..>2534
                     /locus_tag="VFL49_11115"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET7912573.1"
                     /translation="MARFSSDQETRLVAAFFGNIKGYYVEVGANDPRERSQTWHLEQQ
                     GWTGVLVEPQPDLAARLRARRKAKIYEAACSSAENAGQELPLHVAGPLSSLDRRSMAP
                     GATPEKIIRVHVRTLDSILEEAGSPAAFDFLSIDVEGHEIKVLQGFDIARWRPRLIMI
                     EDHVADLSKHRYLISSG"
BASE COUNT          453 a          770 c          841 g          470 t
ORIGIN      
        1 aaagaaaaaa tgccgccgca ttgggtcggg ttgaaatttt tcaatgtcta cggtccgaac
       61 gaatatcaca aaggacacat ggcgagcgtt ctcgcgaaag tgtttgactt ggcgaagtca
      121 ggccaaccgg tgaagctgtt caagtcgcac cgccagggca ttgccgacgg cgatcagcgc
      181 cgcgatttca tttacgtgga cgatgcggtt tccgtcctgc ggtggctgct cgaggcgagc
      241 ggcatcaatg gcattttcaa tgtcggcacc ggcaaggccg agagcttccg cgacatgatc
      301 ggggccatgt tcaggtcgct gcgtatgccg gcaaatatcg aatatatcga catgccgcag
      361 gcgatccgcg gccagtacca gtatttcacc gaggcgcgcg tcgagcggtt gcgccgcgcc
      421 ggttacaatg ccggtttcac gccgctggaa gccgcggtcg gccagtacgt caccggctac
      481 ctcgaccgcg acgaccgtta tcggtaggcc catgtacgac ttcgacaagc atctttcggc
      541 gctcggcagg cagacgattc tttgcgtcgg cgacctgatg ctggacgaat tcgtgtacgg
      601 cgacgtatcg cgcatctcgc cggaagcacc gacgccggtg attgcggtaa agcgcaccga
      661 cttgatgatc ggcggtgccg gcaacgtcgc gcgtaatctc gtggcgctcg gcgcgcgctg
      721 cattttcgtc ggcgcggttg gcgatgacga ggcgggaggc gccctgacca aggcgctcgg
      781 cacccatccc ttgatcgaat ttcacctggt gatcgacggc aagcggcaga cgacccgaaa
      841 ggtccgtttc gtctccgagc accactcgac acaccttttg cgcgccgatt gggaaacacc
      901 gtctgccgtc gatgccggca gtgaggacgc gctgatcggc cacgttatca aagccatggc
      961 acgcgtgggt gcagttgttc tgtccgacta cgccaaggga gctctgacgc cgcgcgtcat
     1021 tcgcgcggtg attgacgccg cgaatgagcg cggcaaaccg gtcgttgtcg atccgaaggg
     1081 cagcgactac ggcatctacc gcggggcgac gctgatcacc ccgaaccgcc aggagctggc
     1141 cgatgcgacg cgccggcccg caggtaccga tgatgaggtg gcggctgccg caaatgaact
     1201 acgcgatgcg ctcggaacgg aggcggttct cgtgacacgc agcgaagccg gcatgactct
     1261 ggtgggcgac ggcgcgcccg tgcacgtgcc tgcctatccg gtgcgcgtgc gggatgtttc
     1321 gggtgccggc gacactgtcg tggccgtcgt ctcggccatg ctggcgatgc aggcggattt
     1381 cgaggccgcc atgcgtgcgg ccaatgcggc cgccgccgtg gtggtcggca agcgtgggac
     1441 cgcgacggca tcggtcgctg agttgcgctc gcggattttg ccggcggcga cacttgcgcc
     1501 cgaagaaaag atcgtttttg actggggggt tctcgacgac cacctgtcgg actggcgcag
     1561 gcaggggctt cgggtcggct tcaccaacgg ctgcttcgat ctgttgcacc ctggtcacgt
     1621 caagttgctg gccggggcgc gtgccgcctg cgaccggctg gtcgtcggcc tcaatggcga
     1681 tgcctcggtg acgaggctca aaggcgccgg ccggccggtt cagccggtgc aggcacgcgc
     1741 agaggtgctc gccgcgctcg aggccgtcga cctcgttgcg gtgttcgacg aggacacgcc
     1801 ggagaagctg atcgcacacg tcaaaccgac ggtccttgtg aagggcgcgg attatacccg
     1861 cgaacaggtg gtcggccgcg aaatcgtcga ggctcttggc ggcgagatta tcctgattga
     1921 tctggtgccg ggccattcaa ccagctcgat ggttgaacgc acgcgcgcgc cgaaatcgcg
     1981 ctcggcttga gagcttccac ccttggcgcg attttcgtcc gaccaggaaa cgcggctggt
     2041 ggccgccttt ttcggaaata tcaaaggcta ctacgtcgag gtgggcgcca acgatccgcg
     2101 cgaacgctca cagacctggc atctggagca gcagggctgg accggtgttc ttgtcgagcc
     2161 gcagccggac cttgccgcca ggctgcgcgc ccgacgcaag gcaaaaatct acgaagccgc
     2221 ctgctcgtca gcggaaaatg cagggcagga gttgccgctg cacgtcgcag ggccgctgtc
     2281 gtcgctcgat cggcgcagca tggcgccggg agccactccg gaaaaaatca tccgcgtgca
     2341 tgtccgcaca ctcgacagca ttcttgagga ggctggcagc cccgctgcgt tcgatttcct
     2401 gtcgatcgac gttgaagggc acgaaatcaa ggtgctgcag ggtttcgaca ttgcgcgctg
     2461 gcgcccgcgc ctcatcatga tcgaggatca cgtcgcggac ctgtcaaagc acaggtacct
     2521 gatttcgtcc ggtt
//
LOCUS       DASSRL010000548         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1186943, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000548 DASSRL010000000
VERSION     DASSRL010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186943"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VFL49_11120"
     CDS             <1..1521
                     /locus_tag="VFL49_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET7912574.1"
                     /translation="SRSRAKADVQPELHALTHPEIRAIVLGIMLAMFLGALDQTIVAT
                     ALPTIGRHFGNLEDLSWVVTAYLLTATAVTPLYGKLADIHGRRVVMLTAIVIFVTGSV
                     ACALAPNMSALVLGRAFQGLGGGGLMALAQTIIADIVSPRERGRYQGYIGAVFATSSV
                     GGPVLGGFLTEHIDWSLIFWINLPLGLCALGMTSSVLRRVPYHPRKHRLDIVGALLMM
                     AAAIALMLALTWGGRHFEWISPQIGGLLFGSAALWGLFAWRLMAAREPFLPLSVLGNP
                     VVRCATLAGACNMGTLVGMTIFVPLYFEVVLHLSASESGMALIPLMGATVTLSTITGR
                     MMTHVVHYKRMALAGLSLSILSLAPLAIWPTSMPMVLVLLLLMLVGSGLGTVFPISTV
                     CMQNAVSRAQMGIATGAANFFRALFSALVVALLGAIVLGGLGGVTGMSVEMLARTASA
                     PELAYAFRFVFLACGLILSFGMAFLISMEERPLKGPSAPSQAITAPTAPATPIPAE"
     gene            complement(1584..1904)
                     /locus_tag="VFL49_11125"
     CDS             complement(1584..1904)
                     /locus_tag="VFL49_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912575.1"
                     /translation="MRKLLIAAGFAAIIASPASAQSYSSGFGTGNVINLPALEHGGFF
                     ADTASSAYAYAPENPTRRGARDVRTEALSPNDPDTVYEAGQYVGRDPDPNVRLQLRRD
                     WTHE"
     gene            2334..2702
                     /locus_tag="VFL49_11130"
     CDS             2334..2702
                     /locus_tag="VFL49_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7912576.1"
                     /translation="MSLDNDVGPQRVAARGGLAGWQRKRVLEFIEDHLPETISLSTLS
                     ALAQLSPYHFARAFKQDLGLPPHRYHINRRVERAKSLLVDRSVTEVALAVGYAETSSF
                     SSSFRRVTGVSPSEFRRALA"
     gene            complement(2765..>2994)
                     /locus_tag="VFL49_11135"
     CDS             complement(2765..>2994)
                     /locus_tag="VFL49_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HET7912577.1"
                     /translation="EEILFREQAGDRSLVLFDAGDEITVQAGDRGIRFLLVSGKPIQE
                     PVAWYGPIVMNTQTELQQAVSELRSGTFIKP"
BASE COUNT          532 a          888 c          898 g          676 t
ORIGIN      
        1 tccaggtcgc gagcaaaggc agacgtacaa cctgagttgc acgcgctgac gcacccggaa
       61 atcagggcga ttgttctcgg catcatgcta gccatgttcc tcggggcact cgaccagaca
      121 atcgtcgcaa ccgcgctgcc gacgatcggc cggcatttcg gcaaccttga ggacctgtcc
      181 tgggttgtaa cggcctatct tctaaccgcg acggcggtga cgccgctcta cggaaagctc
      241 gccgacatac acggacggcg cgtcgttatg ctcaccgcga tagtcatttt cgtgactggt
      301 tcggttgcgt gtgcgctggc gccaaatatg tccgccctgg tgctcggccg cgcgtttcaa
      361 gggctcggcg ggggcggctt gatggcgctc gcccaaacga tcattgccga cattgtgtcg
      421 ccgcgggagc gtggccgcta tcagggttac atcggtgcag tatttgcgac gtcgtccgtg
      481 ggcggaccgg tactcggcgg gtttctgacc gaacatatcg actggtcgct gatcttctgg
      541 atcaacttgc cgcttggtct ttgcgccctc ggaatgacct cgagcgtgct gcggcgggta
      601 ccgtaccacc cgcgcaagca caggctcgac atcgtcggag ctttactgat gatggcggcg
      661 gcaattgcgc tcatgctcgc cttgacctgg ggcggccgcc acttcgaatg gatatcgccg
      721 cagatcggcg ggctgctgtt cggctcggct gcgctctggg gcctgttcgc ctggaggctg
      781 atggcagcgc gcgagccgtt ccttccgctg tccgtcctcg gcaatccggt tgtccgctgc
      841 gcgacgctcg ccggcgcctg caacatgggc acgcttgtcg gcatgacgat ttttgtgccg
      901 ctttacttcg aagtcgtttt gcatctgtcg gcaagcgaat ccggcatggc actgattcct
      961 ctgatgggtg ccacggtcac cctttcgacc atcacgggcc gtatgatgac gcacgttgtc
     1021 cattacaagc gcatggcatt ggctggcctc tcgctctcga tcttgtcgct ggcgccgttg
     1081 gcaatttggc cgacgtccat gccgatggtg ctggtcctcc tgctgttgat gctggtcgga
     1141 tcgggactcg gcaccgtatt cccgatctcc accgtctgca tgcagaacgc ggtatcgcgc
     1201 gcccaaatgg gaatcgcgac aggcgcggcg aacttcttcc gtgcgctgtt ctcggctttg
     1261 gttgtcgcgc tgcttggcgc catcgttctt ggtggtctcg gtggtgtaac gggcatgtct
     1321 gtcgaaatgc tggcccgcac ggcatcagct cccgagcttg cctacgcttt tcgctttgta
     1381 tttcttgcct gcgggctgat cctgtcgttc ggcatggctt ttctcatttc gatggaggag
     1441 cggccgctca agggaccctc cgcgccttcg caagccataa ccgctcccac ggcgccggca
     1501 actcctattc ccgccgagta gtgagtgatt gcgcaaaaag ccccggcgcg aaaccggggc
     1561 tttgttgtgg cggtgaacgg cctttactcg tgggtccaat cgcgacgtag ctgcagtcgc
     1621 acgttcggat cgggatcgcg gccgacatac tgtcccgcct catagactgt gtcgggatcg
     1681 ttcggcgaaa gtgcttccgt acgaacatcc cgcgcaccac gtctggtggg gttctccggc
     1741 gcataagcat aggcgctgct agcggtgtct gcaaagaaac cgccatgctc gagcgctggc
     1801 aaattgatga cgtttccggt gccaaaaccc gaactgtaag attgggccga tgccggtgaa
     1861 gcaattattg cggcgaatcc cgcggcgatg agcagctttc tcatctggta tctccatgtg
     1921 ttgttgcagc attgtcgctg gccggcgctt agcggccggc ccgaacaacg tggtcactca
     1981 tgcgcggctt cacttgcttg aaattgctag ttcccgacgg caatgcgtgc acggaatggc
     2041 aggtttgaac ctgccactca cgcaggcgtg aattttcgta acccacgcgc gtcatattta
     2101 ggtgttggag ttgggacaga atcaaaacaa accaattttc tcaaaagctg tgatggaaac
     2161 ttccaggatg gcgatggaac aatgcaagtg aaggatacct catgccttgt tgcgctaccg
     2221 gcgacaacca tcgctgtcgc cccaacggcc aatcgcgcga taaaaagtcc caactagagt
     2281 cgaaatcacc ggaacaggat gcgcccctcg gcatactgcc gattcacggg ccagtgagcc
     2341 tcgacaatga tgttgggcct caacgtgtgg cagctcgcgg gggccttgcc gggtggcagc
     2401 gtaagcgggt gcttgaattc attgaagacc acctcccgga aacgatcagt ctttctacgt
     2461 tgtcggcgct cgcgcaattg agcccttacc actttgcgcg tgctttcaag caggatttgg
     2521 ggctgccgcc gcaccgctac cacataaatc ggcgcgttga gcgtgcgaag agtcttcttg
     2581 tcgaccgatc tgtcacggaa gtcgcattgg cggtcggcta cgcggaaacc agttcgtttt
     2641 catcgagctt tcgcagggta accggagtgt ctcctagcga atttcgccgc gcgctggcgt
     2701 gaagcaaaag cccggagctg gtggaagtta cgagctaaag actcgtaccg tgtgaatttg
     2761 ccgattaggg cttgataaat gttccgctgc gcaactcgga gacagcctgc tgtagttccg
     2821 tctgcgtatt catcacaatc ggaccgtacc aggcgacggg ctcctggatg ggtttgcccg
     2881 acaccagcag gaaacggatg ccgcggtccc cggcctgcac cgtgatctcg tcgcctgcgt
     2941 caaacaggac gagagagcgg tcgcctgcct gctcccggaa cagaatttcc tcac
//
LOCUS       DASSRL010000549         7590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_871988, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000549 DASSRL010000000
VERSION     DASSRL010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871988"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFL49_11140"
     CDS             complement(<1..321)
                     /locus_tag="VFL49_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HET7912578.1"
                     /translation="MNGARSADMPLAPPPPDFSGWYLRGDIGFTNQSVDSLFNSNYSG
                     FLSVANVDKGFDAAPLFGLGIGYTINNWLRVDVTGEYRGNANFHGMDVGALPAGGFAS
                     DRYVA"
     gene            complement(591..1934)
                     /gene="glmM"
                     /locus_tag="VFL49_11145"
     CDS             complement(591..1934)
                     /gene="glmM"
                     /locus_tag="VFL49_11145"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7912579.1"
                     /translation="MSRRYFGTDGIRGRANRVITPELALKVGQAAGLIFKNGDHRHRV
                     LIGKDTRLSGYMIETALVAGFTSVGMDVLLTGPIPTPAVGMLTRSMRADLGVMISASH
                     NPFDDNGIKLFGPDGYKLSDETEEKIEELVDSDLGKQLAKSGDLGRARRIDGVQDRYI
                     EFAKLTLPKAVSFEGLRVVIDCANGAAYRVAPGALWEMGADVIPIGVDPDGFNINKDC
                     GSTDLRALCAKVREMRADIGIALDGDADRVMIVDERGHVVDGDQLLAVIAESWKQDNR
                     LQRAGVVATVMSNLGLERYLGGLGIELVRTPVGDRYVLDHMRSHGYNIGGEPSGHIIL
                     SDYTTTGDGFIAALQVLAVIRRNDKPVSEVCHRFEPLPQVLKNVRYQKGKPLENADVR
                     SAIATAERKLGGGGRLIVRPSGTEPVIRVMGEGDDRTLVESVVNDVVEALSEAAA"
     gene            2180..3349
                     /locus_tag="VFL49_11150"
     CDS             2180..3349
                     /locus_tag="VFL49_11150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7912580.1"
                     /translation="MKTITCIEDLRALARRKVPKAFFEYADSGSYNEETLRANRADLE
                     TIKLRQRVMVDVSDRSLATTIIGQKVSAPFALAPIGLCGMQYGDGEILSAQAAEEAEI
                     PFILSTMSINSIEQVAEATTKPFWFQLYVIRDRGFSKDILSRAAKAGCNALVLTVDLQ
                     VLGQRHRDIRNGMTVPPEIRLKNIVDIATKPGWVFSILKGKSKTFGNLAGHVKGMDDV
                     TSLAQWTNSQFDPALNWKDVDWIKKTWPGKLIIKGILDADDAKTAVKLGADAIVVSNH
                     GGRQLDGAPSSISALPAIAQAVGSDTEVLFDGGIRTGADMLRALALGARACLIGRAYI
                     YGLGAGGKAGVAKAIDILKKELSVAMALTGTTRIGDIGPQVLVGDETKKRRTRKS"
     gene            3401..4711
                     /locus_tag="VFL49_11155"
     CDS             3401..4711
                     /locus_tag="VFL49_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HET7912581.1"
                     /translation="MSAEVSIGDGKGARLTPHPLRAAILGEVHARPFTPIETPRRVLH
                     FAFDTDGDAARRDRAALADFCANRNLDPLKPSAKQHRVTLGGTMLRWEQHSEFTTYTW
                     ELPSPGAMPFHPDAASLAAPMAGLPQPGPVLVALDLHLLAEKKERQPIANLFDRASLA
                     VAENSDGLALFATDFQADAAGFVRILVLDRGLGSERAGAVVQRIIELETYRTLALLGL
                     PEAQRLLPSINRIEKRLGEVTEEMRRTDKLIDNHRLLDELTALASELEAGATASLFRF
                     GASRAYNEIVHLRLQTIGERKVEGLPTWSSFLARRMAPAMRTCITTEERQANLSEKLA
                     RAANLLRTRVDVELEQQNRDLLRSMNERTRLQLRLQATVEGLSVAAISYYVVGLFTYL
                     VKGAHDEGVPVDVSLATALFVPAAVLAIWWVVRRVRRKHIAETD"
     gene            4768..4992
                     /locus_tag="VFL49_11160"
     CDS             4768..4992
                     /locus_tag="VFL49_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912582.1"
                     /translation="MQDAAHALTQQFLAWVAESPRSYADADAWRRSCPHLSIWEDAIA
                     DGLVRFENGSSMKESRIVLTARGRKRLDGN"
     gene            5156..5893
                     /locus_tag="VFL49_11165"
     CDS             5156..5893
                     /locus_tag="VFL49_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HET7912583.1"
                     /translation="MIEQLRQRLQTLQKTSGIKGRPSTLPFGIPAIDTTLGGGLTNEL
                     HEIAATGEAHLPAATGFALGIARRSVLSPRLFWIIEDMALAESGVPHGAGLDAFGLSP
                     EQLVMVSAAHRRDLLWAMEEALRCSAINAVIGEMRVGAIDGVAIRRLSLAAAESGTLA
                     LLLRTEPPGDASTAATRWVVGAVPSTSLGAPRLAAQLVRNRRGPTGTWILQWSDGDGQ
                     FILATHALPLAAPALDRSHRQVAQDAA"
     gene            5826..7364
                     /locus_tag="VFL49_11170"
     CDS             5826..7364
                     /locus_tag="VFL49_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET7912584.1"
                     /translation="MLCLWLRRLSTDRIARSRKTLHEIAAPLVVTGKRGNVEVLTAVN
                     DSARRFGLTPGLALAQARVMHPGIDVVAENVDADATLLGSVADWCLRYTPLVACDRAD
                     GLLLDVSGCAHLYGGEHELIADLSGRLEKSGFAYSLAIAGTIGAAWATAHYGEPGIYA
                     CGEERSILAPLPLSALRLPPATVTSLARVGLKRIGDILDLPRAPLTARFGPEVLRQLD
                     RALGHEHEPLTPRLPVAPYVAEQRFPEPIGREEDVLAITVRLARRLQFALEQGGDGVR
                     RIELSLFRTDGVVRHIAAGTSRPLRDPEDIRALFVERLAALADTLDPGFGFDMVRLSV
                     LVVEPCPPEQIGIAGGEEAAELCRLIDRLSARLGPRRVRRLVAQDSHIPELAETALPA
                     QAVNGDAGWRAFRRYRNEIDLPPRPLRLLARPEPIEAVAEVPDGPPLRFRWRRALHEV
                     IAAEGPERIEGAWWSEHGGPARDYFRVEDKSGLRFWLFRAGLYRDLAHGAGAPAWFLH
                     GTFA"
     gene            complement(7369..7590)
                     /locus_tag="VFL49_11175"
     CDS             complement(7369..7590)
                     /locus_tag="VFL49_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HET7912585.1"
                     /translation="MIRRARPASKVFPNLQFPKPHGRGPQREPVKVQTTIRLDRDVIE
                     YFRRRGRGWQTRLNSALRDVISRKRKARG"
BASE COUNT         1438 a         2372 c         2306 g         1474 t
ORIGIN      
        1 tgcgacatac cggtcagacg cgaagccgcc ggcaggaagc gcgcccacgt ccataccgtg
       61 gaagttcgcg ttgccgcgat attcacccgt cacgtcaacg cgcagccagt tattgatggt
      121 gtagccgatg ccgagtccga acagaggagc agcgtcgaag cccttgtcga cattcgctac
      181 cgacagaaag ccggaatagt tgctgttgaa aaggctgtcg acgctttgat tggtgaagcc
      241 gatgtcgccg cgaaggtacc agcctgagaa atccggcggc ggtggcgcca gcggcatgtc
      301 ggctgagcgt gctccgttca tgacggcaaa tgaagcaact gccgccagtg ccaaagttcg
      361 aatacgcacg ataatcatgg tcgtccctca caactgacgc agccgccccg gctaccgttc
      421 gaagagacga tggcaccaaa tccttaattg ccgcttaacc ttaattctta accacgaccg
      481 ttaacgcgcg gccttgtact gcgagcggat gcgccgcacg caggcgaaat ctgcttttaa
      541 gaaattgttt agatcggtcg tttgtcaacg attgcggcga cagcgagcct tcacgccgcc
      601 gcctcgctca gagcttcgac gacatcgttg accaccgatt cgaccagagt cctgtcgtcg
      661 ccctcgccca tcacacggat gaccggctcg gttcccgatg gccgcacaat caggcggccg
      721 cccccgccga gcttgcgttc agcagtcgcg atcgccgagc gcacgtctgc gttttcgagc
      781 ggcttgccct tctggtagcg cacgttcttc agcacctgcg gcagcggctc gaaacgatga
      841 cagacctccg atacgggctt gtcgttccgc ctgatcacgg cgagaacctg caaggcggca
      901 ataaatccgt cgccggtcgt cgtgtaatcc gagagaatta tgtgtccgga cggctcgccg
      961 ccaatgttgt atccgtgcga gcgcatatgg tcgagcacgt agcggtcacc gaccggcgtg
     1021 cgcaccagtt cgataccaag cccgccgaga tagcgctcaa ggccgagatt cgacatgacg
     1081 gtcgcgacga cgcccgctcg ctgcagccgg ttatcctgct tccagctttc cgcaatgacc
     1141 gcgagaagct gatctccatc gaccacgtgt ccgcgctcgt caacgatcat gacgcggtcg
     1201 gcatcgccat cgagtgcaat gccgatatcg gcacgcatct cgcgaacctt ggcgcaaagg
     1261 gcacgcaggt cggtcgatcc gcaatctttg ttgatgttga atccatccgg atcaacaccg
     1321 atcggaatga cgtctgcacc catttcccag agcgcgccgg gcgccacgcg ataggcagca
     1381 ccgttggcgc agtcgatcac cacgcgtagg ccttcgaacg acaccgcctt gggcaatgtc
     1441 agcttggcga attcgatgta gcggtcctgc acgccatcga tgcgccgggc gcgccccaga
     1501 tcgccgctct tggcgagctg cttaccgaga tctgaatcga ccagctcttc aatcttttct
     1561 tccgtctcgt cgctgagttt gtacccatcg ggcccgaaca gtttgatgcc attgtcatcg
     1621 aacggattat gcgacgctga aatcatgacc ccgagatcgg cgcgcatcga gcgtgtcagc
     1681 atgccgaccg ccggcgtcgg gatagggccc gtcaggagca catccatccc cactgaagta
     1741 aaccctgcga ccagcgcggt ttcgatcata tagcctgaga gccgggtatc cttgccgatc
     1801 agaacacggt gccggtgatc gccattcttg aaaatcaggc cggccgcctg cccgaccttg
     1861 agcgcaagct cgggcgtaat cacccgattg gcccggcctc gaattccgtc ggtgccgaaa
     1921 tagcgacgac tcatacgatt acccccggcg cccgaccctt ttttggatgc ccgtcacctg
     1981 gccgatcgaa cgcggtcagg tctttctcgt ggctgcataa taatgtcaac gcagtgaatt
     2041 tgcttcaaaa attgtaggga atcattaccg cacgcggtta accgttttca ccgcctggac
     2101 cgcctgaaaa ccgactattt cgcagcggcg caaggctgtg ctacccggta tgtttaccgt
     2161 gtgatcctca gaacgtctca tgaagaccat tacctgcatc gaggacctgc gagcgcttgc
     2221 ccgccgcaaa gttccaaaag ctttttttga atatgccgac agcggttcgt acaacgagga
     2281 aacgttgcgt gcgaaccggg ctgatctcga aaccatcaag ctgcgccagc gcgtgatggt
     2341 cgatgtctcc gaccgaagtc tcgccaccac gatcatcgga caaaaagtga gtgcgccatt
     2401 tgcgttggct ccgatcggtt tatgcggcat gcaatacggc gacggcgaga tcctgtcggc
     2461 ccaggccgcc gaagaagccg aaattccgtt catcctgtcc accatgtcga tcaactcgat
     2521 cgagcaggtg gcggaggcga ccacaaagcc attctggttt cagctttacg tcatccgcga
     2581 ccgcggtttt tccaaggata tattgtcacg cgcggccaag gccgggtgca atgccctggt
     2641 tctcaccgtc gatctgcagg ttctcggcca gcgccatcgc gatatccgca acgggatgac
     2701 ggtgccgccg gaaatccggc tcaagaacat cgtcgacatc gcaaccaagc cgggatgggt
     2761 gttcagtatc ctgaagggca aaagcaagac gttcggcaat cttgccggcc acgtgaaggg
     2821 gatggacgac gtaacctcgc ttgcgcagtg gaccaatagc cagttcgatc ccgcgctgaa
     2881 ctggaaagat gtcgactgga tcaagaaaac ctggccgggg aagctcatca tcaagggcat
     2941 cctcgatgcc gacgacgcaa agaccgccgt aaagctcggc gccgatgcca tcgtcgtttc
     3001 caaccacggc ggccgccagc ttgacggcgc gccctcatcg atctcggcgc tgccggcaat
     3061 cgcgcaggcg gtcgggtcgg acactgaagt gctgttcgac ggcggcattc gcaccggcgc
     3121 cgacatgttg cgcgcgctgg ccctgggcgc gcgggcctgc ctgatcggcc gtgcctatat
     3181 ttacggcctc ggcgccggcg gcaaggccgg ggtcgccaag gcaatcgata tcctgaagaa
     3241 agagctcagc gtcgcgatgg cgctgacagg cacaacccgc attggcgata tcgggccaca
     3301 ggtgcttgtg ggcgacgaga cgaagaagag gcgtacgcgg aagagctgac aaggaaagcc
     3361 ttcggctggc gatcgtggcg ctgcgtgcgg cggggatatc atgagcgctg aggtatcgat
     3421 cggtgatggc aagggcgcac ggctcacgcc gcatcctctg cgcgcggcaa tcctcggcga
     3481 ggttcatgcg cggcccttca caccgatcga aacaccgcgc cgcgtcctcc attttgcctt
     3541 cgacactgac ggtgatgccg ccaggcgcga ccgcgcggct ctcgccgatt tctgcgcgaa
     3601 ccgcaatctc gatccgctaa agccctccgc caaacagcat cgcgttacgc tcggcggcac
     3661 aatgcttcgg tgggaacagc attcggagtt cacgacatat acctgggagc tgccgtcgcc
     3721 gggcgcgatg ccgtttcatc ccgatgcagc atcactggcg gcacccatgg ccggactgcc
     3781 gcaaccggga ccggtgctgg tggcgctgga tctgcacctc ctggccgaga aaaaggaaag
     3841 gcagccgatc gcgaatttgt tcgatcgtgc aagcctggcg gtcgcggaaa attccgatgg
     3901 ccttgccttg tttgcgaccg acttccaggc cgatgcggcg ggctttgtgc gcatcctggt
     3961 gctggaccgc ggcctgggca gcgaacgcgc cggagctgtg gtccagcgca tcatcgagtt
     4021 ggagacctac agaacgcttg cgttgcttgg cctgccggaa gcgcagcggt tgctgccttc
     4081 aatcaacagg atcgaaaagc ggctcggtga agtaaccgag gaaatgcgca ggaccgataa
     4141 gctcattgat aaccatcgcc tgctcgacga attgactgcg ctggcctccg aactcgaggc
     4201 tggtgcaacc gccagcctgt tccggttcgg cgccagccgc gcctacaacg agattgtcca
     4261 tttgcgtctg caaaccattg gcgagcgcaa ggtggaaggc ttgccgacct ggtcttcgtt
     4321 tctggcacgg cgcatggcac cggccatgcg cacctgcatc accaccgagg agcggcaggc
     4381 gaatttatcc gagaagctgg cgcgcgccgc caacctgctg cgaacccggg tcgatgtcga
     4441 actggagcag caaaatcgcg atctgttgcg ctcgatgaac gagcgcacgc ggctgcaact
     4501 gcggctgcag gcaacggtcg aaggactttc ggtggcggcc atcagttatt acgtcgttgg
     4561 gctgtttacc tatctcgtca agggtgccca cgacgaaggc gtgccggtcg acgtctcgct
     4621 cgccaccgcc ctgttcgtgc cggccgccgt tctcgccatc tggtgggtcg tgcgccgcgt
     4681 gcggcggaaa catatcgcgg agacggatta ggcaggcgcg catggcgagc ctgacataat
     4741 gcggggcgcg ggcttatact ggttgttatg caagacgctg cacacgcatt gacgcagcaa
     4801 ttccttgctt gggtggcgga atcgccgcga agctatgccg acgcagatgc ctggcgccgg
     4861 agttgcccgc atctgtcgat ttgggaagac gccatcgccg atggattggt ccggttcgag
     4921 aacggcagca gcatgaagga atctcggatc gtgctgacag ctcgcggacg caagcgtctc
     4981 gacggcaact gatcgcctgg gcggatccag ggctggttcg tagcccctaa aaacctcact
     5041 gcctggcgac ttgacgtcct ctgatcattg gaagacacta gaacaaacca agaacataag
     5101 ctgagacggg ccagccaact ggaccgaatg tattagcgtc ttcaaaggtt taagaatgat
     5161 cgagcaactc cgccagcgcc ttcagacctt gcaaaagacg tcagggatca agggccgtcc
     5221 cagcaccctg cccttcggta ttcccgctat cgatacgacg ctcggcggag gcctcaccaa
     5281 cgaactccat gaaatcgcgg ccactggcga ggcacatctt cccgcagcga ccggatttgc
     5341 tttggggata gcgagacgtt ccgtcctctc acctcgtctg ttttggatca tcgaggatat
     5401 ggcgctcgcc gagagcggcg tgccgcacgg tgccggtctt gatgcctttg gcctctcgcc
     5461 ggagcaattg gtgatggtgt ccgccgcgca ccgacgcgac ctgctctggg caatggaaga
     5521 agcgctgcgt tgcagcgcga ttaatgcggt gatcggtgaa atgcgcgtcg gcgccatcga
     5581 cggcgtagcg atacggcgac tctcactcgc ggcagcagaa agcggcacat tggctttgct
     5641 gctgcggaca gaacctccgg gcgatgcttc gaccgcagcc acgcgctggg tcgtcggtgc
     5701 agtgccttcg accagcctcg gcgcaccgcg ccttgccgcg caacttgttc gcaaccgccg
     5761 cggcccgact ggaacatgga ttctccaatg gagcgacggc gatgggcaat tcatcctcgc
     5821 aacgcatgct ctgcctctgg ctgcgccggc tctcgaccga tcgcatcgcc aggtcgcgca
     5881 agacgctgca tgaaatagcc gcaccgctgg tcgtcaccgg caagcgtggc aatgttgaag
     5941 ttctcacggc cgtgaatgat tcagccagac ggttcggcct cacgcctggc cttgccttgg
     6001 cgcaagcgcg cgtcatgcat cccggcattg acgttgtcgc tgaaaatgtc gacgccgatg
     6061 cgactttgct cggctctgtt gccgactggt gtttacgcta cacgccgttg gttgcatgcg
     6121 atagggccga tggcctgctg ctcgacgtca gcggttgcgc gcatctttat ggcggcgagc
     6181 acgaactgat cgccgatctc agcggccgcc tggaaaaatc cggttttgcc tattcgcttg
     6241 ccattgccgg cactattggc gctgcctggg ctaccgcaca ttacggcgaa cccggaatct
     6301 atgcttgcgg cgaggaacgc agcattctcg caccattgcc gctgtccgca ttacgactcc
     6361 cgccggcgac tgtcacgagt ctcgcccgtg tcggtttgaa acgcatcggt gacattctcg
     6421 atctgccccg cgcgccgctg accgctcgtt tcgggcccga ggtgctgcgg cagctcgatc
     6481 gcgcgcttgg ccacgagcat gaaccgttga cgccgcggct gccggtcgcg ccctatgttg
     6541 ccgagcagcg ctttcccgag ccgattggcc gtgaagagga cgtgctcgcg atcaccgtcc
     6601 gactggcgcg gcgactgcaa tttgcgctgg agcagggcgg cgatggcgta cgccgcatcg
     6661 aattgagcct gttccgcacc gacggtgtag tgcgccatat cgccgccggc acctcgcggc
     6721 cattgcgcga tccggaggat atccgggcgc tgttcgtcga gcggctcgcc gcgctcgccg
     6781 acacgctcga tcccggtttc ggcttcgata tggtgcggct gtcggtcctc gtcgtcgagc
     6841 cctgccctcc cgaacagatc ggcatcgccg gcggggaaga agcggccgaa ctctgccggt
     6901 tgatcgaccg gctgagcgcg cggctgggcc cgcgccgtgt ccgccgtttg gtcgcgcagg
     6961 acagccacat cccggaactg gccgagaccg ctttgccggc acaggccgtt aatggcgatg
     7021 ccggctggag agcattccgc cgctatcgca acgagatcga cctgccacca cgtccgttgc
     7081 ggctgctggc acggccggag ccgatcgaag cggtggcgga agtgccggat ggcccgccgt
     7141 tgcgcttccg gtggcgacgc gcgctgcacg aggtgattgc ggccgaagga ccggaacgca
     7201 tcgaaggtgc ctggtggagc gagcatggcg gaccggcacg cgattacttc cgtgtcgagg
     7261 acaaaagcgg gcttcgattc tggctgttcc gtgccgggct ataccgcgat ctcgcgcacg
     7321 gcgccggtgc gccggcgtgg ttcctgcacg ggacatttgc gtgatgaatt atcctcgtgc
     7381 tttgcgtttg cgtgagatca cgtcccgtaa cgcgctgttc aatcgtgttt gccagccgcg
     7441 tcctcgccgt ctgaaatatt cgatcacatc gcgatcgagc cgaatggtcg tttgcacctt
     7501 gactggctct ctttgcggac cccgaccgtg tggtttggga aactgcaagt tcggaaagac
     7561 cttcgacgca ggccttgccc gccgtatcat
//
LOCUS       DASSRL010000550         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1676555, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000550 DASSRL010000000
VERSION     DASSRL010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676555"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFL49_11180"
     CDS             complement(<1..503)
                     /locus_tag="VFL49_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7912586.1"
                     /translation="MPLSELLAVLLVVAVIVALMVGYPVALTLAGASLVFAVLGDILG
                     AMSFGILGALPQRIFGVMTNEVLLAIPLFIFMGVMLERSRIAEDLLETMGRLFGALRG
                     GLGISAVIVGTLLAAAKGVVGATTVTMGLIVLPTMLRHGYDKKLAAGTVAATATLAQI
                     FPPATVLV"
     gene            complement(503..1003)
                     /locus_tag="VFL49_11185"
     CDS             complement(503..1003)
                     /locus_tag="VFL49_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET7912587.1"
                     /translation="MTAVADVIDRVVSGVGRAVSWLVLVVVLLQFALVVARYLFGLGS
                     IWLTETVIYAHAAVFMLAAAWTLGVGGHVRVDVFYAEASGQTKALIDLLGALLLLLPF
                     MLVLVWLSVPYAARSWAILEHSQEASGLPLVFVLKTFIPVFALLMALQGVAQAIRAAA
                     VLARAR"
     gene            1066..1692
                     /gene="nthA"
                     /locus_tag="VFL49_11190"
     CDS             1066..1692
                     /gene="nthA"
                     /locus_tag="VFL49_11190"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET7912588.1"
                     /translation="MSDHPHHEHDHSELSETQLRVRALETVLTEKGYVDPAALDLLIE
                     TYEKKVGPRNGARVVAKAWTDPAYRARLLKDATPAIAEIGYAGRQGEHIVALENTPKR
                     HNMVVCTLCSCYPWPVLGLPPVWYKSAPYRSRAVSDPRGVLADFGVTLPKDTEIRIWD
                     STAEIRYLVLPMRPPGTEGWSEDKLAALVTRDSMIGTGLPKSPKDVAK"
     gene            1689..2348
                     /gene="nthB"
                     /locus_tag="VFL49_11195"
     CDS             1689..2348
                     /gene="nthB"
                     /locus_tag="VFL49_11195"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HET7912589.1"
                     /translation="MNGGQDLGGAHGHGPVEPEPNEPVFHGEWEKRAFALTLAMGMPG
                     GWNIDMGRFARENRPPGEYLSMTYYQIWFAALETMLKERGLVAEDEIVAGHSLHPPKP
                     VKRVLSPGDVAKTLHRGGPTERGTNTAATFKAGDKVRAKNINPVTHTRLPRYVRGRVG
                     TIERVIGYHVFPDSNATGAGEDPHWLYTVRFEGRELWGEEGDPTSKVSVDAWEPYLEP
                     A"
     gene            2350..2733
                     /locus_tag="VFL49_11200"
     CDS             2350..2733
                     /locus_tag="VFL49_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HET7912590.1"
                     /translation="MGMDPQAAMRAAVAVPGVPRDTDGPVFREPWEAQAFAMALALHE
                     RGVFTWPEWAAALAAEIKRAQAAGDPDTGETYYSHWLNTLEKLVAEKGVTTADTLHRY
                     RDAWDRACDRTPHGQAIELRPDDFR"
     gene            complement(2730..>3342)
                     /locus_tag="VFL49_11205"
     CDS             complement(2730..>3342)
                     /locus_tag="VFL49_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HET7912591.1"
                     /translation="AALLGATAKAMGDTVRIIEIVSYGLIVLIGLRLLWVKGRAFLQS
                     LGLWKGQHDHDHAHGHVHDHHHHHDHNHDASAWTHGHAPEPSELAGDHWLKRGLTAII
                     AVGLRPCSGAIIVLVFALAQGLFWIGVASTFVMGLGTAITVAAIATLAVGARGFAGRL
                     AKARPGTGMIVLRGLETAAALVIVLFGAALLTGYLASERLMGF"
BASE COUNT          697 a         1096 c         1038 g          511 t
ORIGIN      
        1 accagcacgg tggcgggcgg gaaaatctga gcgagcgtcg cggtggccgc gacagtaccc
       61 gcggcgagct tcttgtcgta gccgtgccgc aacatggtcg gcagcacgat caggcccatc
      121 gtcacggtcg ttgcaccgac cacgcccttg gccgccgcaa gcaatgtccc cacgatgacc
      181 gcagaaatgc cgagcccgcc gcgcagcgcg ccgaacagac gtcccatggt ctcgagcaaa
      241 tcctcagcga tgcgcgagcg ctccagcatc acgcccatga agatgaagag cggaatggcc
      301 aacagcactt cattcgtcat aacgccgaag atgcgctgcg gcagcgcgcc gagaatgccg
      361 aagctcatcg cacccagaat gtctccaagc acggcgaaga ccagtgatgc gccggcgagg
      421 gtcagcgcca ccggatagcc aaccatcaag gcgacgatga ccgcgacgac caggagaact
      481 gcaagcagtt cggagagagg catcagcgcg ccctcgccag caccgcagcc gcgcggatgg
      541 cctgcgccac gccctgcaag gccatcagca gcgcgaagac gggaatgaac gtcttgagaa
      601 cgaacacgag cggcaggccg cttgcttcct gcgagtgctc gagtatcgcc catgagcgcg
      661 ccgcataggg caccgacagc cagaccagaa cgagcatgaa tggcagcagc agcagaaggg
      721 cgcccagaag gtcgataagc gccttggttt gccccgacgc ctccgcataa aagacatcca
      781 cccttacgtg cccgcccacg ccgagggtcc aggctgccgc cagcatgaat accgccgcgt
      841 gggcgtagat gaccgtctct gtaagccaga tggaaccaag cccgaacagg taacgggcca
      901 cgacgagcgc gaactgcagc agcaccacga caaggacaag ccaggagacc gcacggccca
      961 cgcccgaaac gacgcggtcg ataacgtcgg ccaccgcagt catatgcgca aatctcataa
     1021 ctccgatgat ggcataaatg ccaaaccttt gcttatagtg ggaccatgtc cgatcaccct
     1081 catcacgaac acgatcattc cgaactttcg gaaacgcagc ttcgcgtgcg cgcactcgaa
     1141 acggtgctga ccgaaaaggg ctatgtcgat ccggccgcgc tcgacctgtt gatcgagacc
     1201 tacgaaaaga aagtcgggcc gcgcaacggc gcgcgcgtcg tcgcaaaggc ctggaccgat
     1261 ccggcctatc gtgcgcggct gctcaaggat gccacccccg cgattgccga aatcggttac
     1321 gccggccggc agggcgagca cattgtcgcg ctggagaaca cgccgaaacg gcacaacatg
     1381 gtcgtgtgca cgctgtgctc ctgttaccct tggccggtgc tcgggttgcc accggtctgg
     1441 tacaaatcgg cgccctaccg ctcgcgcgcg gtgtccgatc cgcgcggcgt gctcgccgac
     1501 ttcggcgtca cgctcccgaa agatacggaa atccgcatct gggattcgac cgccgagatt
     1561 cgttacctgg tgttgcccat gcgccctccc ggcaccgagg gctggagcga ggacaagctt
     1621 gcagcgcttg tcacgcgcga ttcgatgatc ggcacgggct tgccgaaatc gccgaaggac
     1681 gttgcgaagt gaacggcggc caggacctcg gcggagcgca cggccatggc ccggtcgagc
     1741 cggagccgaa cgagcctgtc tttcacggcg aatgggaaaa gcgcgccttc gcactcaccc
     1801 tggccatggg catgcccggt ggctggaaca tcgacatggg acgcttcgcg cgcgaaaacc
     1861 gtccgcccgg cgaatacctg tcgatgacct attaccagat ctggtttgcc gcgcttgaga
     1921 caatgctgaa agagcgcggc cttgtggcgg aagacgaaat tgttgccggt cattcgttgc
     1981 atccgccaaa accggtcaag cgggtgttat cgccgggcga cgtcgcgaag acgctgcacc
     2041 gcggcggccc gaccgagcgc gggacaaata cggcggcgac gttcaaggcg ggcgacaagg
     2101 tccgcgcgaa gaatatcaac cccgtgactc acacgcggct gccccgttac gtgcgcggcc
     2161 gtgtcggcac gatcgagcgg gtcatcggct atcacgtctt tcccgacagc aatgcgaccg
     2221 gagccggcga ggatccgcac tggctttaca cggttcgctt cgagggtcgc gagctgtggg
     2281 gtgaagaagg cgacccaacg tcgaaagtgt ccgtcgatgc ctgggagccg tatctggagc
     2341 cggcctgata tgggaatgga cccgcaagct gccatgcgcg ccgcagtcgc cgtgcccggt
     2401 gtgccgcgcg acaccgacgg cccggtattt cgcgagccgt gggaggcgca ggctttcgcg
     2461 atggcgttgg cgctgcacga acgcggcgtt ttcacgtggc cggaatgggc ggcggcgctt
     2521 gcggccgaaa tcaagcgcgc gcaggcagcc ggcgatcccg acaccggcga gacctactac
     2581 tcccattggc tcaacacgct cgagaaattg gttgcggaaa aaggtgtgac gaccgcggat
     2641 actttgcatc gctaccgcga cgcctgggac cgcgcctgcg accgcacccc gcacggccag
     2701 gcgatcgaac tccgccctga cgatttccgc tagaagccca tcaggcgttc gcttgccaaa
     2761 taaccggtga gcaaggccgc cccgaacagc acaatgacaa gagccgccgc ggtttccaat
     2821 ccgcgcagga ctatcattcc ggttcccggc cttgcttttg ccagcctgcc tgcgaaaccc
     2881 cgtgcaccca ccgccaaagt ggcaatcgcc gccaccgtga tcgccgtccc caatcccatc
     2941 acaaaggtcg aggcgacgcc gatccagaac aatccttgcg cgagcgcaaa cacgagcacg
     3001 atgatcgcgc ccgaacacgg gcgcaggcca acggcgatga tcgccgtcag gccacgcttc
     3061 aaccagtgat cgccggcaag ttcgctcggt tcgggtgcgt gcccatgggt ccaggctgat
     3121 gcgtcatggt tgtggtcgtg gtgatggtgg tgatcatgta cgtgtccgtg cgcatggtca
     3181 tgatcgtgct gacccttcca caggccaagc gactgcagaa atgcccggcc tttcacccac
     3241 aggaggcgca ggccgatcag gacaatcagc ccatagctca cgatctcgat tatgcggacg
     3301 gtatcgccca tggccttggc ggtcgcgcca agcagcgcgg cg
//
LOCUS       DASSRL010000551         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_714246, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000551 DASSRL010000000
VERSION     DASSRL010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714246"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFL49_11210"
     CDS             <1..243
                     /locus_tag="VFL49_11210"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HET7912592.1"
                     /translation="KVTTGAIESYAIEIMATANLFRRGHRICLEIMSADMPTGVGGAT
                     NAEYVPNHICSSRTTLHKIYHDAQRPSHLLLPVIPQ"
     gene            265..855
                     /locus_tag="VFL49_11215"
     CDS             265..855
                     /locus_tag="VFL49_11215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7912593.1"
                     /translation="MSEARQIASVKPGPEIKRFRRIVTGHNAQGRSVILCDDTSPHVM
                     PIMSQPNFAVTDFWKTNATPADNGTTTADDPCRLPIQVAPPAGGSVFRVVQFPPDKDW
                     AAKAAAMGGSVAIDETAKAAHTSGPVRHAHMHRTRSLDYAIVLSGEIWAVMDDGETKM
                     TAGDILIQRGTNHAWANRSNAPCVVAFVLIDAKPLD"
     gene            887..1888
                     /locus_tag="VFL49_11220"
     CDS             887..1888
                     /locus_tag="VFL49_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7912594.1"
                     /translation="MKPHPKNVGDHLYVAVLLPFDRRMKVDEPAYRRFLQHFLKNEKF
                     VRMGGGLCINPEAGEIFYLTRQEKRRVLEIAMEEINRKVPVLAGTWAMTTDETVETAR
                     DCKALGVDGIFVTPPGGAQDVTSCWDADNYPEIWLDQIIAQDRAVDLPIITHPVGGAK
                     PPFYPGLPLEATLRICREVPNVVGWKMTYMYDGFRLIAKGLRGLDRHVAVMGALASRF
                     HEYKATGLFDGTLSGFWNFALEPMLDHLEAWDAKDIDKARRIWDGGLVQLHEYVADMG
                     RLHIRYKTATWLRGLIPNPFMRSPMPKPKQAEIDTLYQLLKDLGLPVINIKETRLAA"
     gene            complement(1863..>2050)
                     /locus_tag="VFL49_11225"
     CDS             complement(1863..>2050)
                     /locus_tag="VFL49_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912595.1"
                     /translation="DVSHPAAAAALPSKTERLVTAEAARWTSAPHANRGGFPAARSKA
                     LQARQVCMHITRPGAFL"
BASE COUNT          409 a          642 c          637 g          362 t
ORIGIN      
        1 aaggtcacga caggggcgat cgaaagttac gcaatcgaga tcatggcgac cgccaacctg
       61 ttccgccgcg gccaccgcat ttgcctggaa atcatgagcg ccgacatgcc gaccggagtc
      121 ggcggtgcga ccaatgccga atacgtgccc aaccacatct gcagcagccg caccacgctg
      181 cataagattt accacgacgc ccagcggccc tcgcatctgc tgctgcccgt catcccgcaa
      241 taacggcctc gtttggagtt ccgcatgagt gaagctcgtc aaatcgcgtc ggtaaaaccg
      301 gggcccgaga tcaagcgctt ccggcgtatc gtcacgggcc ataacgcgca aggccgctcg
      361 gtgatcctgt gcgacgacac atcaccgcac gtgatgccga tcatgagcca gccgaacttc
      421 gcggtgaccg acttctggaa aacgaatgcg acgcccgccg acaacggtac gacgaccgcg
      481 gacgatccct gccggctgcc gatccaggtg gcaccgccgg cgggcggcag cgtgttccgg
      541 gtcgtgcagt ttccgcccga caaggattgg gccgcgaagg cggcggcgat gggcggctcg
      601 gtcgccatcg atgaaaccgc gaaagcggcc cataccagcg gcccggtccg gcatgcccac
      661 atgcatcgta cgcgctcgct cgattacgcc atcgtgctgt cgggcgaaat ctgggcggtg
      721 atggacgacg gcgaaacgaa gatgacggcg ggcgacattc tgattcaacg cggcaccaac
      781 catgcctggg cgaaccggtc gaacgcgcct tgcgtcgttg cgttcgtgct catcgacgcc
      841 aagccactcg attgagaatt taacaaatca aacgttggag ttgaccatga agccccaccc
      901 caagaacgtc ggcgaccatc tttacgtggc cgtgctgctg ccattcgacc ggcggatgaa
      961 ggtcgatgag ccggcctatc gacgtttcct gcagcacttc ctgaagaacg aaaagttcgt
     1021 tcgcatgggc ggcgggcttt gcatcaatcc ggaggccggc gaaatcttct atcttacgcg
     1081 gcaggagaag cggcgcgtgt tggaaatcgc catggaggag ataaaccgga aggtgccggt
     1141 tctcgccggt acctgggcga tgacgaccga cgagacggtc gagacggcgc gtgactgtaa
     1201 ggcattgggt gttgacggca tcttcgtgac gccgccgggc ggagcgcagg acgtcacctc
     1261 gtgctgggat gctgacaatt atccggaaat ttggctcgat caaatcattg cgcaagatcg
     1321 cgcggtcgat ctaccgatca tcacgcatcc ggtcgggggc gccaagccac cgttctatcc
     1381 gggcctgccg ctcgaagcca cgctacgcat ctgccgcgag gtgccgaacg tggtcggctg
     1441 gaagatgacc tacatgtacg acggcttccg gctgatcgcg aaagggttgc gcgggctcga
     1501 ccgccatgtc gccgtgatgg gtgcgctcgc ctcgcgcttc cacgaataca aggcaactgg
     1561 cctcttcgac ggcacgctct ccggcttctg gaatttcgcg ctcgagccca tgctcgatca
     1621 tctggaagcc tgggacgcga aggacatcga caaggcgcgc agaatttggg acggcgggct
     1681 tgtgcaactg cacgaatacg tcgccgacat ggggcgatta cacatccgct acaagacggc
     1741 gacttggctg cgcgggctca ttcccaatcc gttcatgcgc tcgccgatgc ccaagcctaa
     1801 gcaggctgag atcgacacgc tctaccaact gctgaaggat ttgggccttc cggtcatcaa
     1861 catcaaagaa acgcgcctgg ccgcgtgatg tgcatgcaca cttgccgcgc ctgcagcgcc
     1921 tttgagcggg ccgccgggaa gccgccacgg ttcgcgtgcg gtgccgaagt ccagcgtgcg
     1981 gcttccgctg tgacaagacg ctcggtcttg ctcgggagtg cagccgccgc cgcagggtgg
     2041 ctcacgtctt
//
LOCUS       DASSRL010000552         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_994347, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000552 DASSRL010000000
VERSION     DASSRL010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994347"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFL49_11230"
     CDS             complement(<1..399)
                     /locus_tag="VFL49_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912596.1"
                     /translation="MRSRLAPIPVVDVRNGGPVRHAVENHVRAQALRDECIRWLPRVA
                     AGLLPAIDSMTRRWLLRSCSPYVEEVEAIADELDFSGIWFLNGCYQWGCTALSREQDG
                     MCWIARTLDWPFPGLGRHVEIAHMQGAAGEF"
     gene            complement(532..1365)
                     /locus_tag="VFL49_11235"
     CDS             complement(532..1365)
                     /locus_tag="VFL49_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7912597.1"
                     /translation="MAIHFNQRTIAPIVAADGIARQPLLNEQRVPGIRFELDRLTIPP
                     GGRGKLTTVPGELAWFQMTAGTATIHAAGRALPVATNHIGLLPPNFEGMLASDGGATL
                     LFARVPNAERLDPSIGMDPPPFRVIDWQHEPLLQSEHDARKRIYIVTPKMFGTRAIRG
                     EMIIYPPGTECPVHHHQGGAHFMFFLAGEGTCYAGADQVMSVRSGDVIYYHDLEPHWV
                     KGGTDGDLIFSEFFVPSAVATVWADPSKVCTWIPTGLNYRGGRASRLIEKHAHSHLAD
                     V"
     gene            complement(1437..2681)
                     /locus_tag="VFL49_11240"
     CDS             complement(1437..2681)
                     /locus_tag="VFL49_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912598.1"
                     /translation="MTKLTRVLPLLCVAAASAMPAQAQELKIGFLAPRTGIITQLGTD
                     MVNGFQMYLDEHDGKLGGATVKFIVEDDKGTVAGDVAMAKKLILQDKVDMIVGAVLGS
                     ASYALAPISNQEKTLFIGTITTGDDIAQRQSDKFPYTVFSTWVSSQPTHALGQWACEQ
                     GYKRVSAVGTDYAFGYEQVGGFQKTFEDCGGKIIQKIWVPFGNKDFGPYIPSIKRDVD
                     AIFSLMVGPMTLQFPKQLRAAGNKTIILGGSTNYDEFALPFMGDEALGDISAHVYSAA
                     LPTPANEAFVKKYRAKYGKVPSYYSEANYATAMWIDKTLEKFKGKYPGPADFIKAMAS
                     IEINPPRGPVKLDLATKSPIQNIYVKKVEKKKMFGYDKDELWNTVLKTYTDVGTYWQY
                     DPAKFRAQPVYSRDFPPCKFCE"
BASE COUNT          571 a          856 c          828 g          618 t
ORIGIN      
        1 gaattcgccg gcggcgcctt gcatatgcgc tatttccaca tgacggccga gccccggaaa
       61 cggccaatca agtgttcgag cgatccagca cattccgtcc tgctcgcggg atagcgcggt
      121 acaaccccac tggtagcagc cgttaagaaa ccagatgcct gaaaagtcga gctcgtccgc
      181 tatggcctcg acctcctcca catatggcga gcacgatcgc aggagccagc gtcgggtcat
      241 cgaatcgatt gccggcaaca gcccggccgc aacacgcggt aaccagcgaa tgcactcgtc
      301 acgcagagcc tgtgcgcgca catggttttc gaccgcgtga cgaacggggc caccgttccg
      361 cacatcgaca acaggaatgg gcgcaagtct cgatctcaac tgtcggctcc cgacatgaat
      421 gcattttacc gcgcttcatc gcggcagcca tagcttgcag cacggaaatt gcggctgtag
      481 ccgcgaagcg ccgcattgcg cctcgcgcgg tcaagaattg ccggttcctt tttagacgtc
      541 ggcaagatgg ctgtgggcgt gcttttcgat cagtcgggac gccctgccac cgcgatagtt
      601 caaccctgtc ggaatccacg tgcagacctt cgaagggtcg gcccacacgg ttgcaaccgc
      661 gctcggcaca aagaattccg agaagatcag gtcgccgtct gtgccaccct tcacccagtg
      721 cggctcaagg tcgtggtaat aaatgacgtc accggatcgc accgacatga cctgatcggc
      781 tcccgcgtaa caggtgcctt ccccggcgag gaagaacatg aaatgggcgc ctccctggtg
      841 atggtgtacc gggcattcgg tgccgggcgg atagatgatc atttcgccac ggatggcccg
      901 ggtcccgaac atttttggcg ttacaatata aatgcgcttg cgagcgtcgt gctccgattg
      961 cagtaacggc tcgtgctgcc agtcgatgac acgaaaaggc ggcggatcca tgccgataga
     1021 cggatcgagt ctctcggcgt tgggcacccg agcgaacaat agcgtcgcgc cgccatcgct
     1081 tgcgagcatt ccctcaaaat ttggaggcag caggcctata tggttggttg cgaccggcaa
     1141 cgcccgtccc gcggcgtgaa tagtcgctgt gccggcggtc atctgaaacc aggcgagttc
     1201 gccgggtaca gtcgtgagct tcccgcggcc gcctggcgga attgtcaggc gatcgagttc
     1261 gaaccgaata cccggcacgc gctgctcgtt caacaatggc tggcgtgcaa tgccgtctgc
     1321 cgcaacaatc ggcgcgatcg tcctctggtt aaaatgaatc gccatctgtc acggttaccg
     1381 aacagcccgg ccctcctcac ttcaagattg gtaccaccac ccttctcgac ccgcacctat
     1441 tcgcagaatt tgcaaggcgg aaaatcgcgg ctgtagaccg gctgcgccct gaactttgcc
     1501 gggtcgtatt gccaatatgt gccgacgtcg gtgtaagtct tgagcaccgt gttccagagc
     1561 tcgtccttgt catagccgaa catcttcttc ttttcgactt tcttgacata aatgttctgg
     1621 atcggactct tggtcgcgag atcgagcttg accggaccac gcggcggatt gatctcgatg
     1681 cttgccatcg ctttgatgaa gtcggccggc cccggatact tgcctttgaa cttctccagc
     1741 gtcttgtcga tccacatggc ggtcgcgtaa ttggcttccg aataatagga cggcaccttg
     1801 ccgtatttgg cgcggtattt cttgacgaaa gcttcattgg ccggcgttgg gagggccgcg
     1861 ctgtagacgt gcgccgagat atcgccgagg gcctcgtcac ccatgaaggg cagggcgaat
     1921 tcgtcgtagt tggtgctccc gcccaggatg atcgtcttgt tgccggcggc gcgtagctgc
     1981 tttgggaatt gcagcgtcat cggtccgacc atcaacgaga agatggcgtc gacgtctctt
     2041 ttgatgctgg ggatgtacgg cccaaagtct ttgttgccga acggcaccca gattttctgt
     2101 atgatcttgc cgccgcaatc ctcgaatgtc ttctggaacc cgccgacctg ctcatagccg
     2161 aaggcgtaat cagtgccgac cgcggaaacg cgcttataac cttgctcgca agcccattgc
     2221 ccgagcgcat gtgttggttg cgacgaaacc caggtggaga atacagtata aggaaatttg
     2281 tcggactggc gctgcgcaat gtcgtcgccc gtggtgatgg tgccgatgaa gagcgtcttt
     2341 tcctggttcg aaatcggcgc cagcgcgtaa gacgccgatc cgagcaccgc gccaacgatc
     2401 atgtccactt tgtcttgaag gatgagcttc ttggccatgg ccacgtcgcc ggcgacggtg
     2461 cccttgtcat cctcaacaat gaacttaacc gtagcgccgc cgagcttgcc gtcatgctcg
     2521 tccaggtaca tctggaagcc gttgaccatg tcggtgccga gctgcgtgat gatgcccgtg
     2581 cgcggcgcga gaaatccgat cttgagctcc tgcgcctgtg ccggcatcgc gcttgcggcg
     2641 gcgacgcaca gcagtggaag tacccgggtc agcttggtca tcgtcgatcc cctcttacaa
     2701 agtcacattt attcaatcac ctcatagtaa cgatcagtct ttctgagcct agcaattcac
     2761 accggcgctc tgaaagaggc cggtcgtctc agtcaatcac gtcgccggcg agagcgggta
     2821 atggcggtgg taagtggaga ccaagtgcct ttccggtgcc gagatcgatc acc
//
LOCUS       DASSRL010000553         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1606697, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000553 DASSRL010000000
VERSION     DASSRL010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606697"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..313)
                     /locus_tag="VFL49_11245"
     CDS             complement(14..313)
                     /locus_tag="VFL49_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HET7912599.1"
                     /translation="MDDKTRTELEAAVYRRLVEHLRSRTDVQNIDLMNLAGFCRNCLS
                     NWMKDAADEKGLAISKDQSREMVYGMPYDEWRAKYQKEASAEQKVAFEKSSPRHS"
     gene            407..1495
                     /locus_tag="VFL49_11250"
     CDS             407..1495
                     /locus_tag="VFL49_11250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7912600.1"
                     /translation="MKFAHFSHVWNKPGMTPHQRYEELWRELQLCDELGYDYAFCVEH
                     HFRPDESWISSPSVYIVGAAARTRKIRVGSMGYIVPLYHPLRLAEEIALVDQMVGGRM
                     ELGLVPGINADYFKPFGFDYNLRKSPTLEFVDYMRTAYGETQPFSFHGDIHRTESALL
                     AVQPAQKPHPPLWMMSRDPQTLEFCARNAINPGYFLVYPRVDAAPRYRKFLADWAVNG
                     HRQRPKIAYCTIVYVDESDQKALDIALPRAARAYEGFLPPAKPGETFEQRAHEHSKKF
                     IGRGEPGASEIMANLFDADYLMKHELVFIGSPRTVTEKIRAAAESGLFNVFMGEFNFS
                     NLPEPELMRSIRMFGEKVIPALRGYEPF"
     gene            complement(1499..2854)
                     /locus_tag="VFL49_11255"
     CDS             complement(1499..2854)
                     /locus_tag="VFL49_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912601.1"
                     /translation="MAEVVQVSRLLDQRGLSSFHYSLIFWCVLLSLIDGYDIAAIAFA
                     APHLVREWGLKPGQLGPVFSASLVGILFGSALFGWIGDRYGRKIALISSNLLFGVFTL
                     AAAHSTNLEQLFWLRLLAGLGIGGVIPNIVAINTESAPRKYKATLALIAVGFVPIGGA
                     IPGVVAATLVPAHGWQILFTIGGIVPIVLAVIGLFTLPESIKFMALHESQRAKMEKTI
                     AAIDPNFKVPPNATYVIEDEQQFPGFNPVHLFRQGLHLITPLLWLLFALNLMGYFFLL
                     SWTPTLMQMLKLPPSVGALAGAMIQIGGTVGSLLLARWFERRRFFAIAIVFVIAVPVV
                     ASIGFAGTTSTTALAIATFFAGFCVLGIQTGINVAGALVYPTSLRANGSGWELGIGRV
                     GSIIGPLVGALFVGLPVEKLYMWSSLPFALGAVVCFAIWKLNEERLRAHPELQAAQAP
                     A"
BASE COUNT          636 a          935 c          894 g          531 t
ORIGIN      
        1 aagccggcgg tgactaggaa tggcgagggc tggacttttc gaacgcgacc ttctgctcgg
       61 cagaggcctc tttctggtat ttcgcgcgcc actcgtcata cggcatgccg tagaccattt
      121 cacgactttg atccttgctt atggcaaggc ccttttcgtc ggcggcatcc ttcatccagt
      181 tcgacaggca attgcggcaa aagccggcaa ggttcatcag gtcgatgttc tgcacatcgg
      241 tacgcgacct caagtgctca acaaggcgcc ggtagaccgc ggcttcgagc tcggtgcggg
      301 tcttgtcgtc catggcggcc tccttcactg tagaaagata gacacgctct ggccgcgttt
      361 gaagagggct aaaatgccca gccgccgtct gtgaggagag cgcatagtga agtttgccca
      421 cttttcgcat gtctggaaca agccgggcat gaccccgcac cagcgttacg aggaactgtg
      481 gcgcgaactt cagctatgcg acgagcttgg ctacgactac gcgttctgcg tcgagcacca
      541 tttccgcccc gacgaaagct ggatttcgtc gccaagcgtc tacatcgtcg gcgcggctgc
      601 gcgcacccgc aagattcgcg tcgggtcgat gggctatatc gtgccgctct accacccgct
      661 gcggctggca gaggaaatcg ccctcgtaga ccagatggtc ggcggtcgca tggagctcgg
      721 actcgtcccc ggcatcaacg cggactattt caagcctttc ggcttcgact acaacctgcg
      781 taaatcgccg acgcttgaat tcgtcgacta tatgcgcacg gcctacggcg aaacacagcc
      841 gttctcattt cacggcgata ttcaccgcac cgaaagcgcc ctgctcgcag tgcagccggc
      901 acaaaagccg catccgccgc tctggatgat gagccgcgac ccgcagacgc tcgaattctg
      961 cgcgcggaac gcaatcaatc cgggctattt cctggtctat ccgcgcgtcg acgcagctcc
     1021 gcgctatcgc aaattccttg ccgactgggc cgtcaacggc caccgccagc ggcccaagat
     1081 cgcgtattgc accatcgtct acgtcgacga atccgaccag aaggcgctcg acatcgcatt
     1141 gccgcgtgcg gcgcgcgcgt atgaagggtt cctgccgccg gcaaagcccg gggaaacctt
     1201 cgagcagcgt gcccacgagc attcgaaaaa attcatcggc cgcggcgagc cgggtgcctc
     1261 cgagatcatg gccaatctct tcgatgctga ttatctcatg aagcacgaac tggtcttcat
     1321 cggctcgccc aggacggtga cggaaaaaat ccgcgccgca gccgaatcgg gcctgttcaa
     1381 tgtcttcatg ggcgagttca acttctcgaa cttgcccgag cctgagctga tgcgttcgat
     1441 ccgcatgttc ggcgagaagg tcatccccgc cttgcgcggc tacgagccgt tctgacgctt
     1501 atgccggtgc ctgcgcggcc tgcaactcgg gatgcgcgcg caggcgctcc tcgttgagct
     1561 tccagatcgc gaagcagacc accgcgccga gcgcaaaggg cagcgacgac cacatgtaga
     1621 gtttctcgac cggcagaccg acgaacaacg caccaacaag cggcccgatg attgagccga
     1681 cgcgtccgat gccaagctcc cagcccgagc cgttggcgcg cagggaggtc ggatagacca
     1741 gcgcgccggc gacgttgatg ccggtctgga tgccgagcac gcagaagccg gcaaagaatg
     1801 tcgcgatcgc gagcgcggtc gtcgaggtgg taccggcaaa gccgatcgaa gcaacgaccg
     1861 gcaccgcgat cacaaagacg atcgcaatgg cgaaaaagcg cctgcgttcg aaccagcgcg
     1921 ccagaagcag tgagccgacc gtgccgccaa tctggatcat ggcccctgcc agcgcgccca
     1981 ccgacggcgg cagcttcagc atctgcatca aagtcggcgt ccacgacagc aggaagaagt
     2041 aacccatcag attgagggca aacagcaacc acaacagcgg cgtgatcaag tgcagcccct
     2101 gacggaaaag gtggaccgga ttgaaaccgg ggaactgctg ttcgtcttcg atcacgtatg
     2161 tcgcattcgg cggcactttg aaattgggat cgatcgccgc gatggtcttc tccatcttgg
     2221 cgcgctggct ttcgtgcaac gccatgaact tgatcgattc cggcaaggtg aacagcccga
     2281 tcacggcaag cacgatcggc acgatgccgc cgatcgtgaa caaaatctgc cagccatgcg
     2341 ccggcaccag tgttgcggcg acgacaccgg gtatagcgcc gccgatcggc acaaaaccga
     2401 cggcgatcag cgcgagcgtg gctttgtatt ttcgcggcgc cgactcggtg ttgatcgcga
     2461 cgatattcgg aatcacgccg ccgatgccga ggccggccag caggcgcagc cagaacagct
     2521 gttcaagatt cgttgagtgg gctgccgcga gcgtaaatac gccgaacagc aaattcgagg
     2581 agatcagcgc gatcttgcgg ccgtaacggt cgccgatcca tccgaacaaa gccgagccga
     2641 acagaatgcc gacgaggctg gcgctgaaga ccgggccgag ctggccgggc tttaatcccc
     2701 actcgcggac cagatgcggc gcggcaaaag caatggcggc gatgtcgtag ccgtcgatca
     2761 acgagagcag cacgcaccag aaaatcaagc tgtagtggaa cgagctaagc ccgcgttgat
     2821 caagcaggcg cgatacctgc acgacttccg ccatgattgc ctccccacgc gtgttattgc
     2881 cccgccgagc ctttgcggct cttgctacga tgctatgcga gggccgtgcc ggagggaagg
     2941 aggtcgcgtc aggaaaattg cgataccggc aggatcaatt ccgacggatg cgcggc
//
LOCUS       DASSRL010000554         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1747057, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000554 DASSRL010000000
VERSION     DASSRL010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747057"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..414
                     /locus_tag="VFL49_11260"
     CDS             94..414
                     /locus_tag="VFL49_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912602.1"
                     /translation="MRTIFVLLVSGLTILVAGASYAADLRLDRGRVHARHSGTFDQRL
                     RVVEQVPYCGDCEAPIGRTHSANVVRLRFINAPFWQESCAAGACGVYYPVMRTCAFWG
                     LGCI"
     gene            complement(430..2853)
                     /gene="gyrB"
                     /locus_tag="VFL49_11265"
     CDS             complement(430..2853)
                     /gene="gyrB"
                     /locus_tag="VFL49_11265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET7912603.1"
                     /translation="MAEPARKNPSDYGAESIKVLKGLDAVRKRPGMYIGDTDDGSGLH
                     HMVYEVVDNAIDEALAGFATEVTVTLNPDGSCTVRDNGRGIPTGIHKGEGVSAAEVIM
                     TQLHAGGKFDQNAYKVSGGLHGVGVSVVNALSTWLKLTIWRDGKEHFMEFRDGVAKTP
                     LKATGDAKSKRGTEVTFLPSPKTFTMVEFDFATLEHRLRELAFLNSGVAIVLSDQRHA
                     VEKREEMKYEGGVEAFVKFLDRNKTSLISTPIMIKAEREGITVECALWWNDGYHESVL
                     CFTNNIPQHDGGTHLAGFRGALTRQVTQYADTLGASKKEKVALTGDDCREGLTAVLSC
                     KVPDPKFSSQTKDKLVSSEVRPVVENVINQALQDWFEIHPSEAKVIVGKVVEAAAARE
                     AARKARELTRRKGALDVSSLPGKLADCQERDPAKSELFIVEGDSAGGSAKQGRNREFQ
                     AVLPLRGKILNVERARFDKMLSSQEIGTLITALGTGIGRDEFSPDKLRYHKIIIMTDA
                     DVDGSHIRTLLLTFFFRQMEELIKRGHIFIAQPPLYKVSRGKSEQYLKDERALEDYLI
                     MTGAEEAVLRLASGEERAGEDLRKVVDQARSIRNLLNALHSRYNRQVVEQAAILGVLN
                     SEIFGDPKKAKAAAPYIAKRLNALSEETERGWEGEFSEEAGFTFTRTLRGVKTVSIID
                     HALLGSADARKLDEYAGALQKIYEKAGSLRRKDETWAIHGPVGLFEAVTGAGRKGVTM
                     QRYKGLGEMNPEQLWETTLDVDARSLLQVRIKEVDEANVLFDQLMGDLVEPRRQFIQD
                     NALAASVDV"
BASE COUNT          537 a          912 c          920 g          649 t
ORIGIN      
        1 aaatttaacc gcgttcgcaa ataacgccgg cccggaaaaa ggccttttgg ttttgacagc
       61 tatggtcccg ggaatttcgg tggagtgggg ctcatgcgaa cgatattcgt acttctggta
      121 tcgggtctga caattctggt ggcgggcgcg tcttacgccg ccgatttgag gctcgaccgc
      181 gggcgggtgc acgcgcgtca tagcggcacc tttgaccagc ggctgcgcgt cgtcgagcag
      241 gtcccctact gtggcgactg cgaggctccc atcggccgga cgcattccgc caatgtcgtg
      301 cggctgcgct tcatcaacgc gccgttctgg caggagagtt gcgccgccgg cgcctgcggc
      361 gtctactatc cggttatgcg gacctgcgcc ttctggggcc ttggctgcat ctaggccaaa
      421 tccagccgtt caaacgtcca cgctggccgc aagggcgttg tcctgaatga attgccggcg
      481 cggctcgacc agatcgccca tcagctggtc gaacagcacg ttggcttcat cgacttcctt
      541 gatgcgcacc tgcagaagtg agcgcgcgtc tacgtcgagc gtggtttccc acaattgctc
      601 ggggttcatc tcaccgagac ccttgtagcg ctgcatcgtt acgcccttgc gtcccgcacc
      661 ggtaacagcc tcgaacaaac cgacgggccc gtgaatcgcc caggtttcat ccttgcgacg
      721 cagtgagccg gccttctcgt agatcttctg caaagcgccg gcatattcgt cgagcttgcg
      781 ggcatctgcc gagccaagca aagcgtgatc gataatggat acggtcttca cgccacgcaa
      841 agtgcgcgtg aacgtaaagc ccgcctcttc gctgaactcg ccctcccagc cgcgttcggt
      901 ttcctcggag agggcattaa gccgtttggc gatgtaaggg gccgccgctt tcgccttctt
      961 cgggtcgccg aaaatctccg aattgagcac gcccaggatt gccgcttgtt cgacaacctg
     1021 gcggttgtaa cgactgtgca gagcattgag aagattgcgg atggaacgcg cctggtcgac
     1081 caccttgcgc aaatcttcgc cggcgcgctc ttcgccggag gcaaggcgca gcacagcctc
     1141 ttcggcgccg gtcatgatca aatagtcttc cagggcgcgc tcgtctttca aatattgttc
     1201 ggattttccc cgcgagacct tgtagagcgg cggctgcgcg atgaagatgt ggccgcgctt
     1261 gatcagttct tccatctgcc ggaagaagaa cgtcagcagc agcgtgcgga tgtgcgagcc
     1321 gtcgacatcc gcgtccgtca tgatgatgat cttgtggtag cgcagcttgt cggggctgaa
     1381 ctcgtcccga ccgatgccgg tgccgagcgc cgtgatcagc gtaccgatct cctggctcga
     1441 gagcatcttg tcgaaccgcg cacgttcgac gttgaggatc ttgccgcgta gcggcaagac
     1501 ggcctggaat tcgcggttgc gcccctgctt ggcggagccg ccggcgctgt cgccctcgac
     1561 gatgaacaat tcggattttg ccggatcgcg ttcctggcaa tccgcgagct tgccgggcaa
     1621 agatgagacg tcgagcgcgc ccttgcggcg ggtgagttcg cgtgccttgc gcgccgcttc
     1681 acgcgcggcg gcggcttcca cgaccttgcc gacgatgact ttcgcttcgg aagggtgaat
     1741 ttcgaaccag tcctgcaatg cctggttgat cacgttctca accacggggc ggacttccga
     1801 cgacaccagc ttgtccttcg tctgcgaaga gaacttcgga tcgggcacct tgcaggagag
     1861 cacagcggtc aggccctcac ggcaatcgtc gccggtgagc gcgaccttct ctttcttgga
     1921 ggcgccgagc gtgtcggcat attgcgtgac ctggcgggtg agggcgccgc ggaagccggc
     1981 aaggtgcgtg ccgccatcgt gctgaggaat attgttggtg aaacagagca cgctctcatg
     2041 gtagccgtcg ttccaccaca gggcgcattc gaccgtgatg ccctcgcgtt cggccttgat
     2101 catgatcggc gtggaaatga gcgaagtctt gttgcgatca aggaacttca cgaaggcctc
     2161 gaccccgccc tcatacttca tctcctcgcg tttctcgacg gcgtggcgct gatcagagag
     2221 gacgatggca acgcccgagt tcaggaaagc caactcgcgc agccggtgtt ccagggtcgc
     2281 gaagtcgaac tcgaccatgg tgaaggtctt cgggctgggc agaaaggtca cctccgtgcc
     2341 gcgcttgctc tttgcatcgc cggtggcctt gagcggagtc ttcgcgacgc cgtcgcggaa
     2401 ctccatgaaa tgctccttgc cgtcgcgcca gatcgtaagc ttgagccagg tcgacagcgc
     2461 gttcaccacg gagacgccga caccatgcag gccgccggaa accttgtagg cgttctggtc
     2521 gaattttccg ccggcgtgca gttgcgtcat gatgacttcg gccgcggaga cgccttcgcc
     2581 cttgtgaatg ccggtcggga tgccgcgtcc gttgtcgcgc acggtgcacg agccgtcggg
     2641 gttgagcgtc accgtcactt cggtcgcaaa tccggccagt gcctcatcaa ttgcgttgtc
     2701 caccacctca taaaccatgt ggtggaggcc ggacccgtcg tccgtgtcac cgatatacat
     2761 gcccggccgc ttgcggaccg cgtcgaggcc cttgaggacc ttgatcgact cggcgccata
     2821 atcggacgga ttcttgcggg ccggttcagc catcgacggc accctttgaa cagacagaaa
     2881 aatgagtcgg aatgagcgat ttgtgtcgca cgaaaaatgg gccccgtaca gagcaaagtc
     2941 actgggctta atatgttgat ttctcttcgc tttttaaaga ggtggcggtg caaaaatccc
     3001 catttttttt gccgcttt
//
LOCUS       DASSRL010000555         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1082169, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000555 DASSRL010000000
VERSION     DASSRL010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082169"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..972)
                     /locus_tag="VFL49_11270"
     CDS             complement(127..972)
                     /locus_tag="VFL49_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912604.1"
                     /translation="MVEIQDTAPAVAKPSNQAGERARSFSISNLSHGMLAIGSIVLAF
                     LIWQFLSTVLFNPFLVPPPLEVIRTAIPMLMSGEILGDVAISMTRVLVGFATGSLAGI
                     ITGVLLGRIQLLHDLLDPIIELLRYLSPTAMIPIAVIWFGIGELSKYFLIFWGTFFIV
                     VINTTAGVWRAPIARQRAAECLGASQLQIFVLVVIPSAVPYIVTGMRVAMASSFMSII
                     PAEILAADSGIGYLLQKSSMLLQTNRIFVALLTICVLGFIVDRIFRFFVDRVLARYMS
                     FVSQT"
     gene            complement(998..1771)
                     /locus_tag="VFL49_11275"
     CDS             complement(998..1771)
                     /locus_tag="VFL49_11275"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912605.1"
                     /translation="MLRVSNVRKVFESARGTYIALDGVSLTISDGEFLCLLGPSGCGK
                     STLLNILAGFERATAGTVMSGDMPITGAGRERVMFFQDAGSALLPWLTVEENVRFGLR
                     VRKMPKADWQPIIDRYLRMTGLDTHRTKFPSELSGGMRQRLQIARALAVEPAVLLMDE
                     PFGALDAMTRRHMHSILLDIWQRTGKTIVFVTHDIAEAISLGDRIGVMSFGPRSVITK
                     LIDIDLPRPRDLTNPKVAVLYNEIEALLAPDLARVAEHA"
     gene            1981..>2961
                     /locus_tag="VFL49_11280"
     CDS             1981..>2961
                     /locus_tag="VFL49_11280"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7912606.1"
                     /translation="MSDVKPIAPVRIGQTDIVYAQGVRAGSWLFFTGHEATDFEHGIA
                     GPVAGKPGQPLGGAPRYLREGRFIFDRFARLIAAEGGDLRHIVRVDQYYPRAECVNPY
                     QRARKTLLKDYVPPSTSVLMEELLTVGASMNVSLLAVLPGEGREPHAARAEGVPVPQH
                     SGFIASLACGDYVFVAGQMPNNEAMTGLDPKAYRPPNAVWNGTDIRLQTEFLITGRLK
                     PGLEAGGSSLRNAIKAQVYLTDINDLPEFMDVWNCHFADSPCALTVVATKGLALLESK
                     IEINIFGVRDSGKIKKQIIEHKASTHMRLGSAAVRAGDLLCLSALYAADAD"
BASE COUNT          606 a          891 c          933 g          531 t
ORIGIN      
        1 ggctgaccga acaaggtcat cttcagcgga gcattttgcg cctgtgccgc cgtgaggccg
       61 agcatggacg cggctgcgat gcagagaact ttcacggtgt tgtgcatggc tcactctctg
      121 ttggttttac gtctgcgata cgaaagacat gtaacgggca agtacgcggt cgacgaagaa
      181 gcggaagatg cgatcgacga tgaagccgag cacgcagatc gtgagcagcg ccacgaaaat
      241 gcgattggtc tgcagcagca tcgaggattt ctgcagcagg tagccgatgc ctgaatcggc
      301 ggcgagaatt tcagccggga tgatgctcat gaacgaggac gccatcgcga cgcgcatccc
      361 cgtcacgatg taggggacgg ccgacggaat gacgacgagc acgaagatct gcaactggct
      421 ggcgcccagg cattccgccg cgcgctgccg ggcgatggga gcgcgccaca cccccgcggt
      481 cgtgttgatc acgacgatga aaaacgtgcc ccagaagatc aaaaagtatt tcgaaagctc
      541 accgatcccg aaccagatca ccgcaatcgg aatcatcgcc gtcggcgaca gatagcgaag
      601 cagctcgatg atcggatcga gcaggtcgtg cagaagctgg atacggccga gcagcacgcc
      661 ggtgatgatc ccggcaagcg agccagtggc aaatccgacc aggacccgcg tcatgctgat
      721 cgcgacgtcg ccgaggatct cgccgctcat cagcatcggt atggccgtac ggatgacctc
      781 gagcggcggc gggacgagaa acgggttgaa cagtaccgtc gacaggaact gccagatcag
      841 gaatgccagc acgatcgatc cgatcgccag catgccgtgc gacagatttg agatgctgaa
      901 actgcgggcg cgctcgcccg cctggtttga aggcttggcg acggccggcg cggtgtcctg
      961 aatctctacc aaagcgtcac cattgatgtg cccgcggcta tgcgtgctcc gcgacccgcg
     1021 ccagatccgg cgccagcaag gcctcgatct cgttgtagag gacagcaacc ttcgggttgg
     1081 tgaggtcgcg cggtcgcggc aggtcgatgt cgatcagttt ggtgatcacc gagcgcggcc
     1141 cgaacgacat cacgccgatg cgatcgccga gcgatattgc ttccgcaatg tcgtgcgtga
     1201 caaagacgat cgtcttgccg gtgcgctgcc agatatccag cagaattgaa tgcatgtggc
     1261 ggcgagtcat ggcatcgagc gcgccgaacg gctcgtccat cagcagaacc gccggctcga
     1321 ccgccagtgc gcgcgcaatc tgcagacgct gacgcatgcc gcctgacaat tccgagggaa
     1381 atttcgtgcg atgggtgtcg aggccggtca tgcgcaggta cctgtcgatg atcggctgcc
     1441 agtccgcctt cggcattttt cgcacgcgca ggccgaagcg cacgttttct tcgaccgtga
     1501 gccagggcag cagcgccgat ccggcgtcct ggaagaacat cacgcgctcg cgccccgcgc
     1561 cggtgatcgg catatcgccg gacatcacgg tgcccgcggt cgcccgctca aagccggcga
     1621 ggatgttcag cagtgtggat ttgccgcagc ccgacggacc gagcagacag agaaactcac
     1681 cgtcggagat cgtgagagac acgccgtcca acgcgatgta ggtgccgcgc gcggactcga
     1741 acaccttgcg tacgttgctg acgcgtaaca aagctgcctc cggaaagcat gcgaggcgca
     1801 tcccacgacc acttaacgtt atggttggcc ggactcgctg tcaaacgcgt gctgcgtttg
     1861 tctctatgac aattgtcgcg tgcatgcgcg gtctgcggcg catcgcctgc actggacacg
     1921 cgaaatcggc tttgcaatgt ttgacgcaag aaaaatcgcg aatgatcggg gaggttgtgc
     1981 atgtccgatg taaaaccaat cgctcccgtc cggatcggac agaccgacat tgtctatgcg
     2041 cagggcgtgc gcgccggatc ctggctgttt ttcaccggcc atgaggccac cgatttcgaa
     2101 catggcatcg cggggcccgt cgccggaaag ccgggacagc cgctcggcgg tgcgccgcgt
     2161 tatctgcgtg aagggcgatt catctttgat cgcttcgcca ggctgatcgc ggcggaaggc
     2221 ggcgatctgc gccacatcgt gcgcgtcgat caatattatc cacgcgccga gtgcgttaat
     2281 ccctatcagc gcgcgcgcaa gacattgctg aaagattacg ttccacccag cacgtcggtg
     2341 ctgatggagg agctgctcac cgttggcgcc agcatgaacg tctcgttgct tgcggtcctg
     2401 ccgggcgagg gacgcgagcc gcatgccgcg cgcgcggaag gcgttccagt tccgcagcat
     2461 tccggcttta ttgcttcgct cgcgtgtggc gactacgtgt tcgttgccgg gcagatgccg
     2521 aacaacgaag ccatgaccgg cctcgatccc aaagcctatc gtccacctaa cgcggtgtgg
     2581 aacggcaccg acatccggct gcagacggaa tttctgatta ccggacgcct caagccggga
     2641 cttgaagccg gcggcagctc gctcaggaac gcgatcaagg cgcaggtcta cctcaccgac
     2701 atcaacgatc tgcccgagtt catggatgtg tggaactgcc atttcgctga cagtccctgc
     2761 gcgctgacgg tggtcgcaac gaaggggctg gcgctgctcg agtcgaaaat cgaaatcaat
     2821 attttcggcg tgcgcgattc cggaaagatc aagaagcaga tcatcgagca caaagcttcc
     2881 acgcacatgc ggctcggctc cgccgcggtg cgcgccggcg acctgctgtg cctgtcggcg
     2941 ctctacgcgg cggacgccga c
//
LOCUS       DASSRL010000556         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_67158, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000556 DASSRL010000000
VERSION     DASSRL010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67158"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFL49_11285"
     CDS             complement(<1..385)
                     /locus_tag="VFL49_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912607.1"
                     /translation="MKLVLFQISASGDIVPGVLTDRGVVDISSAVSRSYTPQLTMQGI
                     IDGFEKLRPSLERLAANADAIPLEKIRLRPPLPRPGKILACIANYWEHAQREPRPLNM
                     FMKNSDAVIGPGDTIVLPEFTVPWIF"
     gene            549..851
                     /locus_tag="VFL49_11290"
     CDS             549..851
                     /locus_tag="VFL49_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018897849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HET7912608.1"
                     /translation="MRVIAWNTLVEFYTAHPDAKAALTHWYAITKAADWSSMNDVQAA
                     FASGRSLNGERARFEIAGGKYRLIVAFKFRSKIAFIKFVGTHDEYDAVNALTVSKF"
     gene            862..1242
                     /locus_tag="VFL49_11295"
     CDS             862..1242
                     /locus_tag="VFL49_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7912609.1"
                     /translation="MDIRPVRNDGDYRAALVEINRLWSAPEGSEESEKLDILATLVEK
                     YEEQRWPVDQPALDPVDVLHYAIEEMGHTQAELSALLDSRSRASEILNRRRPLTIEMI
                     RAISDAWKIPASLLIRPYRTRTAA"
     gene            complement(1432..1827)
                     /locus_tag="VFL49_11300"
     CDS             complement(1432..1827)
                     /locus_tag="VFL49_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7912610.1"
                     /translation="MIVIDASVLLELLLRTPSAEAVERRLFGGPQESVHAPHLVDVEV
                     TQVLRRYSASGEIDEERGRAALADLADFPIRRYPHDLLLMRVWELRNNFTAYDGVYVA
                     LAEALAAPLLTRDRRLAAAARRHASVELM"
     gene            complement(1824..2054)
                     /locus_tag="VFL49_11305"
     CDS             complement(1824..2054)
                     /locus_tag="VFL49_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912611.1"
                     /translation="MTVMIQIRNVPDVLHRRLKSRAALAGMSLSDYLLSEIREVAERP
                     TLDELRDRLQRRSRVTPSVSPTDAVRLERDRQ"
BASE COUNT          503 a          674 c          680 g          492 t
ORIGIN      
        1 tgaaaatcca cggcaccgtg aattcgggca gcacgatggt gtcgcccggc ccgatgaccg
       61 cgtcggagtt cttcatgaac atgttgagcg ggcgcggctc gcgttgcgcg tgctcccagt
      121 aattggcaat gcaggcgaga atttttcccg ggcgcggcag cggcggccgc aggcggattt
      181 tctcaagcgg gattgcatct gcgttggccg cgagtcgctc aagcgacggc cgcagctttt
      241 cgaagccgtc gatgatgccc tgcattgtga gttgcggcgt gtagctcctg ctgaccgcgc
      301 tggagatatc gacgacacca cggtctgtca gcacgccggg tacgatgtca ccggacgccg
      361 agatctggaa aagaacgagt ttcacggttg tcccctgaca gatgcggaca gccgcaaaag
      421 cctgtacaac cgggagtagt ggcaaggcca cattgcgcgg gtccacatca tgggaacacc
      481 ttcaaatgct tgtttccaat atgagggctt gcctattgtt cccaaattgg gaacatgcta
      541 acctcaccat gagggtaatc gcttggaaca ctttggtgga gttctatacg gctcatccgg
      601 atgccaaggc ggcactgacg cattggtacg cgattacgaa ggccgccgac tggagttcga
      661 tgaatgacgt gcaggccgcg ttcgccagcg gaaggtctct caacggcgag cgcgccaggt
      721 ttgaaattgc tggcggaaag tatcgcctca tcgtggcctt caagtttcgg agcaagatcg
      781 cgttcataaa gtttgtcggc acacatgacg aatacgacgc ggttaacgcc ttgactgtgt
      841 cgaagtttta ggaggccaac tatggatatc cgtccggtcc gcaacgatgg agattatcgg
      901 gctgccctgg ttgagattaa ccgcctttgg agtgcccccg aaggctcgga agaaagcgaa
      961 aagctcgaca ttctggcaac tctcgtcgaa aagtatgaag aacaacgctg gcccgtcgat
     1021 caaccggctc tcgatccggt tgacgtgctt cactacgcca tcgaagagat ggggcacacg
     1081 caagccgagc tttcagcact tctcgattcg cgctctcgcg cctccgagat tctgaatcgc
     1141 cgccgcccac tcacgatcga aatgatccgc gccatcagtg atgcctggaa aatcccagcg
     1201 tcgctgctca tcagacccta taggacacgt acggctgcgt aagattatcc ccgcttcggc
     1261 gggttttctt ttctcgcgtt ttgatttttt tcgcgcgctg cagcgccacg ccgtacctct
     1321 tcaatgcgag cacctgtacc ggtatccgca tctttgcctg ccgctgagca tctgggcgac
     1381 gctccctgtt tcaggacgga aattccgcgg gagggcctct gctgtgagga tttacataag
     1441 ctcgacactc gcatggcgtc tggccgcggc ggccaaccgc cggtcgcgtg tgagcagtgg
     1501 cgcagcaagc gcttccgcaa gcgccacgta gacgccgtca taggctgtga agttgttacg
     1561 caactcccag acacgcatga gaagcaaatc gtgcgggtac cgtcgtattg gaaagtccgc
     1621 gagatcggcg agagcggcgc ggccacgctc ctcgtcgatc tcgccggagg ccgaatatcg
     1681 gcgaaggacc tgtgtcactt caacgtcgac gaggtgtggt gcgtgcactg actcctgcgg
     1741 ccccccgaag agacgccttt ccacggcttc cgctgacggc gtgcgaagaa ggagttccag
     1801 caataccgat gcgtcgatga cgatcactgt cgatcgcgtt caagccggac tgcatcagtc
     1861 ggggagacgg aaggcgttac tctggagcga cgctgcaaac gatcccgcaa ctcatccaat
     1921 gttggtcgtt ccgccacctc gcgaatctcg ctgagcaaat agtcggaaag cgacatgccc
     1981 gccaacgctg cgcgggactt gagccggcgg tgcaacacat cgggcacgtt acgtatctgg
     2041 atcatcaccg tcatgtgctg gacatagaaa catgtgctct acatgtcaat caacttgaca
     2101 ttctagcacc ttggcccaat atgcattact gcataagaac ggtatatgag tgcaaaacag
     2161 catttgaccg aaagtctccc gagcgagcgc caagcgcttg ctttagcggg ctttttcgag
     2221 atcatgagac tttgggacgt cgaaaacgag gacgcgcgac gtattctggg gtcgcccccg
     2281 cttcgcacat tttatgcctg gaagaagaag gcgccggcca aactcccgga ggataccctt
     2341 cgacgcatc
//
LOCUS       DASSRL010000557         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1099863, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000557 DASSRL010000000
VERSION     DASSRL010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099863"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFL49_11310"
     CDS             <1..525
                     /locus_tag="VFL49_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7912612.1"
                     /translation="GVKINDIKRNAFRSHWQELLLPLTGGAQTWVLRDFHSPNLLWLP
                     EREGVARVGLLDFQDAVMGPGAYDLASLLQDARVDMPEAMEIALLSRYVRARMRKGPF
                     EPSDFARAYATLAAQRASKILGIFARLERRDHKPQYLRHMPRVWRYLQRSLAHPGLAS
                     LAAWYRTNVPALKV"
     gene            564..1262
                     /locus_tag="VFL49_11315"
     CDS             564..1262
                     /locus_tag="VFL49_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7912613.1"
                     /translation="MVLAAGLGTRMRPLTDTMPKPLVKIAGKPLIDHVLDRLADAGVE
                     KAVVNVHHFAEQIIDHLGRRAQPRIVISDERGLLLGTGGGVKKALPELGDTPFFHINS
                     DTIWMDGVKPNLARLAETFVAERMDTLLLLAPAEGSIGYAGRGDFAMAADGRLTRRGG
                     RDTVEFVYAGAAILRPALFVDAPGGEFPLTDMFDRAAENGRLFGLKLEGIWMHVGTPA
                     AVAEAEAAIHERTR"
     gene            1305..>4361
                     /gene="addB"
                     /locus_tag="VFL49_11320"
     CDS             1305..>4361
                     /gene="addB"
                     /locus_tag="VFL49_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HET7912614.1"
                     /translation="MIPRVFNIPASAQFLPVLVGALRDGTLVHGFPGSADPLEFSRAT
                     LYLPTRRACRLARDVFLGSLDEEAAILPRIVALGDLDEDEIIFAEAATGELAEEALAI
                     PRAIEPLERRLLLAQLIVRWATLIRPKQGAPLIANTPAAALSLADDLARLIDDVITRK
                     MDWQQLDGLVPDQFDKYWQFTLDFLKIARSYWPERLKEIGAIDAADRRDRLIDAELRR
                     LAGSNAPVIAAGSTGSMPATAKLLAAIAQLPHGAVVLPGLDTDLDEVSWRAIAGSESE
                     KIAPAPVHPQFAMRALLERIGIARDAVRQLASPAPHGRELLVSETLRPAATTDQWRSR
                     FGASDFAAMVDKAMADISVIEAGNAEEEALAIAVSLRETIEAPNKTAALVTPDRALAR
                     RVVAALERWEVEVDDSGGGKLADTPAGLFARLVAEVALEGFEPAPLLALLKHSLLRLG
                     AEAGAHARAVAALERAVLHGPRPRRGSAGLAHALATFKTTRNELHGNDPRSLVMAADL
                     DAAERLAGQLAQALAPLETLGHGLHSLFALAAAHAKALEALASDGKTTAYSAGIDGEQ
                     LQRVFVTIAESETSQTLELAASDYPDVFRLVCSGRAQRRPETGNPRVRIFGLLESRMQ
                     TVDRIVLGGLNEGTWPPDTRSDPWLSRPMRVDLGLNLPELRVGLSAHDFAQALGAPEV
                     VLTRAAKQDGAPTVSSRFFQRLAAVVGAKRWKEAQARGARYMDYARALDHPDKVSPVL
                     RPQPVPPPEARPAQLSVTDIENWLRDPYTIYAKYVLRLFPLEAIDTPPGAADRGTFIH
                     DAIGEFTKTFADALPANVAHELAALGKKHFASLEDYEEARAFWWTRFLRIADWFGGWE
                     RERRGGATKIFAEISGKHRIPLGKTEFFLTARADRIERRADGSYAILDYKTGQPPTEP
                     QVRSGLAPQLTLEAAILRNGGFTGIAPGSVSEIGYVRLKGGDPPGEPKNIKIAQGTPD
                     NCADTALGKLTGIVLKFLVGAEPYRSLVHPMWTKHYGDYDHLARVKE"
BASE COUNT          785 a         1387 c         1380 g          809 t
ORIGIN      
        1 ggcgtcaaga tcaacgacat caagcgtaat gcttttcgat cgcactggca ggagctgctc
       61 ctgccgttga ccggcggtgc gcaaacatgg gtgctgcgtg acttccactc gcccaatctg
      121 ttgtggttgc cggagcgcga aggcgtcgcc cgtgtcgggc tgctcgattt tcaggacgcg
      181 gtgatggggc cgggtgcata tgatcttgcc tcgctgctgc aggacgcgcg cgtcgatatg
      241 ccggaagcga tggagatcgc gttgctgtcg cgctacgtcc gtgcacggat gcgcaaagga
      301 cctttcgagc cgtctgattt cgcacgcgcc tacgccacgc tcgccgcgca gcgcgcctca
      361 aaaatcctgg gcatatttgc gcggctcgag cggcgcgacc acaagccgca atacctgcga
      421 cacatgccgc gcgtgtggcg ctatttgcag cgatcgctgg cgcaccccgg cttggcgtcg
      481 ctcgccgcct ggtaccgtac caatgtgccg gcgctgaagg tctgaggatg ggcgaacccg
      541 tacacagaac gccccgatgc gccatggtgc tggctgccgg gctcggcacc cgcatgcggc
      601 cattgacgga tacgatgccg aagcctctgg tgaagattgc cggcaagcct ctcatcgacc
      661 acgtgctcga tcgtctcgcc gacgccggtg tcgagaaagc cgtggtcaac gtgcatcact
      721 tcgccgagca gatcatcgat catcttggtc ggcgggcgca gccgcggatt gtcatttccg
      781 acgagcgcgg gctgctgctc ggaaccggag gcggtgtcaa aaaggcgctg cctgaacttg
      841 gcgacacgcc gtttttccac atcaattccg acacaatctg gatggacggc gtcaaaccaa
      901 acctggctcg gcttgcggaa accttcgtcg cggaaagaat ggatacactg ttgctgctcg
      961 cgccggcgga aggaagcatc ggttatgccg gccgcggcga tttcgcaatg gcggcggatg
     1021 gccgcctgac ccgccgcggc gggcgcgata ccgtcgagtt tgtctacgcg ggagccgcga
     1081 tcctgcggcc tgctctcttc gtcgacgcac ccggcggtga gtttccccta actgacatgt
     1141 tcgaccgcgc ggcagaaaac ggccgcctgt tcggcctgaa acttgaaggc atttggatgc
     1201 atgtgggaac gccggcagcg gttgccgaag cggaagcggc catccacgaa agaacccgtt
     1261 gatcgacctc aggcattagc gtgtatgatt cgcggatgcc tcacttgata ccccgcgtat
     1321 tcaacattcc cgcttcggca caattccttc cggttctggt cggcgccttg cgcgatggaa
     1381 cgctcgtcca tggatttcct ggttctgccg atccgctcga gttctcccga gcgactcttt
     1441 accttccgac ccgacgcgcc tgtcgtctcg cccgcgatgt tttcctcggt tcacttgacg
     1501 aggaggctgc gattctgccg cgtattgtcg cgcttggcga tctcgatgag gacgagatca
     1561 tcttcgccga agccgccacc ggcgagctgg ccgaggaagc attggcaatt ccgcgcgcca
     1621 tcgaaccgct ggagcgacgg ctcctgctgg cgcagctgat tgtgcggtgg gcgacgttga
     1681 tccgcccgaa acaaggcgcg ccgctgatcg ccaacacgcc agctgctgca ctttcgctcg
     1741 ccgatgatct tgcccgcctg atcgacgacg tgatcacccg caagatggat tggcagcagc
     1801 tggatggcct ggtgccggac cagttcgaca aatattggca attcacgctc gatttcctca
     1861 agattgcccg cagctattgg ccagagcgcc tgaaggaaat cggcgcgatc gatgcggccg
     1921 acaggcgcga ccggcttatt gacgcagagc tgcggcgtct ggcgggcagc aacgcgccgg
     1981 ttattgccgc cggatcaaca ggttcgatgc cggcaaccgc aaaactgctc gccgcgatcg
     2041 cgcagctgcc gcacggcgct gtcgtgctgc ccgggctcga taccgatctc gacgaggttt
     2101 cgtggcgcgc catcgccggc agcgagagcg agaaaatcgc gccagcccct gtccatccgc
     2161 agttcgccat gcgggccttg ctcgagcgta taggcattgc gcgcgacgcc gtacggcaac
     2221 tggcgtctcc ggcgcctcac ggccgcgaac tgctcgtatc ggagacgctg cggccggccg
     2281 caaccaccga tcaatggcga agccgtttcg gcgcttccga tttcgccgca atggtcgaca
     2341 aagcgatggc cgatataagc gtgatcgagg ctggcaacgc tgaagaagag gcgctggcaa
     2401 tcgcggtttc cctgcgcgag acaatcgaag caccgaacaa gactgccgcg ctggtgacgc
     2461 ccgatcgcgc cttggcgcgc cgtgtcgttg cggcactgga gcgctgggag gtcgaggtcg
     2521 acgattccgg aggcggcaag ctcgccgaca cgcccgctgg actcttcgcg cgactcgtcg
     2581 cggaggtagc gctggaaggt ttcgagccgg cgcctctgct ggcattgctc aagcattccc
     2641 tgttgcgcct tggggccgaa gcgggcgccc atgcacgtgc ggttgccgcg ctggagcggg
     2701 ctgtgctgca cggtccgcgt ccgcgccgcg gttccgccgg gctcgcgcat gcgcttgcaa
     2761 cgttcaagac gacgcggaac gagttgcacg gcaacgaccc acgcagcctg gtgatggctg
     2821 ccgacctcga tgcggcggaa aggctggctg gacagctggc gcaagcgctc gccccactgg
     2881 aaacgctcgg tcacggcctc catagcttgt ttgcgcttgc cgcggcacat gccaaggccc
     2941 ttgaggcgct cgccagtgac ggcaagacaa cggcctactc tgccggtatc gacggcgagc
     3001 aattgcagcg cgtgttcgtg acaattgctg agagcgagac atcgcagacg ctggagctgg
     3061 ccgcgagcga ctatcctgac gtcttccggt tggtttgctc aggccgcgcc cagcggcgtc
     3121 cagaaacagg caatccgcgc gtgcggattt ttggcctgct ggaatcgcgt atgcagacgg
     3181 tggaccgcat cgtgctcggc ggactcaatg aaggcacttg gccgcccgac acacgcagcg
     3241 atccctggct cagccgcccg atgcgggttg atctcggtct caatctgccg gagttgcgcg
     3301 tcggcctttc ggcgcacgat tttgcgcaag cgttaggagc gcccgaagtc gtcctcacgc
     3361 gcgccgccaa gcaagacggc gcacccacag taagttcacg gttcttccag agactggcgg
     3421 cagtagtcgg tgcgaagcgc tggaaagaag cgcaggcgcg cggcgcaaga tacatggatt
     3481 atgcgcgcgc cttggatcac ccagacaagg tttcgcccgt cttgcggccg caaccggtgc
     3541 ccccacccga agcacgtccg gcgcaattga gcgtcacgga catcgaaaat tggctgcgcg
     3601 atccgtacac gatctatgcc aagtatgtat tgcggctgtt tcctctggaa gcaatcgaca
     3661 ccccgcccgg cgccgccgac cgcggcacct tcattcatga tgcgattggc gaattcacca
     3721 agaccttcgc cgacgctctg cccgccaatg tcgcgcacga gctggctgcg cttgggaaga
     3781 agcactttgc atctctcgaa gactacgaag aagcgcgcgc tttctggtgg acgcgctttc
     3841 tgcgcattgc cgactggttc gggggatggg agcgcgagcg ccgcggtgga gcgaccaaga
     3901 tcttcgcaga gatttcggga aagcatcgaa tcccgctcgg caaaacagaa ttttttctta
     3961 ccgcgcgtgc cgatcgcatc gagcgccgtg cggacggcag ttatgcgatc ctggactaca
     4021 agaccgggca gcctccaacc gagccgcagg tacgcagcgg gctggcgcct caactcactc
     4081 tcgaggcggc aattctgcgc aatggcggct tcaccggcat cgcgcccggg tcggtatccg
     4141 aaatcggtta tgtgcgtctg aaaggcggcg atcctccggg cgaacccaag aacatcaaga
     4201 ttgcccaagg cacgcccgac aactgcgccg atactgcgct ggggaaactg acgggcattg
     4261 tgctgaagtt tctcgtcggc gccgaaccct accgctcgct cgtgcacccg atgtggacga
     4321 agcactatgg tgactatgac cacctcgcgc gcgtcaagga a
//
LOCUS       DASSRL010000558         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_364496, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000558 DASSRL010000000
VERSION     DASSRL010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364496"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1383
                     /locus_tag="VFL49_11325"
     CDS             178..1383
                     /locus_tag="VFL49_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007802672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912615.1"
                     /translation="MPPHQPRSLFRWREIPGRVWALGLVSLFMDLSSEMIHALLPLYL
                     VTVLGASTLTVGFIEGIAEATASITKIFSGALSDYLGRRKLLAALGYGLAAFTKPIFP
                     LASSIAWLTAGRFVDRIGKGIRGAPRDALVADLTPADLRGTAYGLRQSLDTIGAMIGP
                     FLAIVFMAILADNFTAVFWIAVVPAFVSLAIIVFVVREPALPPDSRTVRSPFSRAELA
                     RLSTAYWLVVAVSAVFTLARFSEAFLLLRAQSVGMQLTFVPIVMVVMNIVYTFTAWPA
                     GALSDRIGRYLVVTAGFAILIVADLVLAFGSGVIAVLIGVALWGLHMGLTQGLLAAMV
                     ADTAPAELRGTAFGMFNLVSGIALLAASIIAGALWDVIGPDGTFFAGALFTTVALAAL
                     PLVRRRVGK"
     gene            complement(1442..1729)
                     /locus_tag="VFL49_11330"
     CDS             complement(1442..1729)
                     /locus_tag="VFL49_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET7912616.1"
                     /translation="MAFFVYIVASKRNGTLYTGMTDDLLKRVWQHRHGIIHGFTRDYH
                     VNKLVWYEIHETRESAIAHERRIKKWNRAWKLELIEKLNPNWRDLSEGLSP"
     gene            complement(1778..2896)
                     /locus_tag="VFL49_11335"
     CDS             complement(1778..2896)
                     /locus_tag="VFL49_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET7912617.1"
                     /translation="MPRFTPHQDNALKSVSDWVKAGPGSNGTPQVFRLFGYAGTGKTT
                     LARHIADEADGEVKFAAFTGKAASVMRGKGCKGASTIHSLIYRARESGEEIPSFDLWD
                     EAPASKAELIIIDECSMVDAELGRDLLSFGVPVLVLGDPAQLPPIQGGGFFTEAEPDA
                     MLTEVHRQAQDDPIVRLSMDVRAGEYLEPGRYGETNVLRKADLDPQRVLEADQVLVGR
                     NVTRRSYNKRMRERHGFEDDMPSAGDKLVCLRNNRKKGLFNGGLWTVKDRGGRKGAKA
                     GIMTMRLLPDDDTATRGVKVSVRPECFTGGIEHVDWQRRKPYDEFDYGYVLTVHKAQG
                     SQWDDVVLFDESFAFPDSRERWLYTGITRAAKKLTVVV"
     gene            3269..4639
                     /locus_tag="VFL49_11340"
     CDS             3269..4639
                     /locus_tag="VFL49_11340"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7912618.1"
                     /translation="MARDQVDMTPIESRDELVTWLEAGCKPKAQFRVGTEHEKFAFTV
                     ADHRPVPYSGKRSIHGLLDGMHSLLGWEPIMEGDNIIGMFDVTGGGAISLEPGGQFEL
                     SGAPYDNVHQTAAELMAHLAQVREVAKPLGIGFLGIGMTPNWTRADMPKMPKGRYKIM
                     TAYMPKVGKLGLDMMYRTCTVQANLDFASEADMVKKLRVSLALQPVATALFANSPFTE
                     GRPNGFLSMRSQIWTDTDNQRAGMLPWAFENGMGFERWVDYALDVPMYFVKRGDKYID
                     VAGKSFRDFFAGKLPELPGERPTISDWANHLSTIFPEVRLKRYLEMRGADGGPWRRLP
                     SLSAFWVGLLYDDDVLEACWDMIKPWTAEERQKLRDDVPKRGFKAQIGNRDALSLADE
                     LLRLCAQGLARRRRLDRNGRDETRYLRPLEESIARGITPAEELLARFHGEWGGSVDPI
                     FEEYAY"
BASE COUNT          841 a         1461 c         1550 g          958 t
ORIGIN      
        1 gcgaagaaga aagaggcggc gtaagcgacg ctgacaaatt tgccgctcac ccccgcgaaa
       61 gcgggggtcc agcgcaccat agcgacgttt ggctggattt ccgcttaggc gtgcggaagc
      121 acgccgtcct tcggacggct atgcggggaa tgagcggaaa aatagagtgc cgcggccatg
      181 cccccacacc aacctcgctc tcttttccgc tggcgtgaaa ttcccggcag ggtctgggcg
      241 ctcgggctgg tctcgctgtt catggatctc tcttccgaga tgatccatgc actgctgccg
      301 ctctatctcg tcactgtgct cggcgcctcg acgctgaccg tcggcttcat cgaaggcatt
      361 gcggaagcga ccgcctcgat caccaagatc ttttccggcg cactcagcga ctatctcggc
      421 cggcgcaagc ttctggcagc tctgggctat ggcctggcag ccttcacgaa gccgatattc
      481 ccgcttgctt cgagcattgc ctggctgacg gcggggcgtt tcgtcgaccg gatcggcaaa
      541 ggcatccgcg gcgcgccgcg cgatgcgctg gtggccgatc tgacgccggc ggatttgcgt
      601 ggcacggctt atggcttgcg gcaatcgctc gacacgatcg gcgcgatgat cggtcccttt
      661 ctggcgatcg tgttcatggc aattcttgcg gataatttca ctgccgtgtt ctggattgcc
      721 gtggtgccgg ccttcgtatc cctggcaatc attgtcttcg ttgtgcgcga gccggcactt
      781 ccgccggatt cacgcacggt gcggtcccca ttctcgcggg ctgaacttgc ccggctgagc
      841 acggcttact ggctggtggt cgccgtatcg gcagttttca cgctggcgcg gttcagcgaa
      901 gcctttctgc ttctgcgcgc gcagtcggtc gggatgcagt tgaccttcgt gccgatcgtg
      961 atggtggtga tgaacatcgt ctacacattt accgcgtggc cggccggtgc attgtcagat
     1021 cgcatcggcc gttacctggt tgtgacggcg ggctttgcga tactgatcgt cgccgatctc
     1081 gtgctggcat tcggaagcgg cgtgatcgcg gtgctgatcg gcgtcgccct ctgggggctg
     1141 cacatggggc tcacgcaagg cctgctcgcg gcgatggtcg ccgacaccgc gccggcggag
     1201 ctgcgcggaa ccgcctttgg aatgttcaac ctggtgagcg gcatcgcgtt gctggcggcg
     1261 agcatcatcg ccggcgcgct gtgggacgtg atcgggccgg acggaacgtt ctttgcgggc
     1321 gcgctgttca caaccgtggc gcttgcggca cttccgctgg tgcggcggcg ggtgggaaag
     1381 tagtgcgaca gaccgacagc acctccgttc gtccccgcgg aagcgggcac ccagactttt
     1441 gctacggcga aaggccttcg ctgagatcac gccaatttgg attgagcttc tcaataagct
     1501 cgagtttcca tgcgcgattc cattttttta tcctgcgctc atgcgcaatc gcggactctc
     1561 gtgtttcatg aatctcgtac cagacaagct tgttcacgtg gtaatcacgg gtgaagccgt
     1621 ggatgatgcc atggcgatgc tgccaaaccc gctttagcaa atcgtctgtc atgcctgtgt
     1681 aaagcgtgcc gttgcgtttg cttgcgacga tatagacgaa gaatgccatg atactctggg
     1741 tccccgcttt cgcggggacg aacggttaaa tgctttttta caccaccacc gtcagcttct
     1801 tcgccgcgcg cgtgatgccc gtataaagcc agcgctcgcg actgtccgga aacgcaaacg
     1861 actcgtcgaa cagcacgaca tcgtcccact gcgagccttg cgccttgtgc acggtcagca
     1921 catagccgta gtcgaactcg tcgtagggct tgcggcgctg ccagtcgaca tgctcgatgc
     1981 cgcccgtgaa gcattccggc cgcaccgaga ccttcacgcc gcgcgtggca gtgtcgtcgt
     2041 cgggcagcag acgcatcgtc atgattccgg ctttcgcgcc tttgcggccg ccgcgatctt
     2101 tcacggtcca gagtccgccg ttgaacagac ccttcttgcg gttgttgcgc aggcagacca
     2161 gcttgtcgcc ggccgacggc atgtcatcct cgaagccgtg gcgctcacgc atccgcttgt
     2221 tgtatgatcg gcgcgtgacg ttgcggccaa ccagcacctg atcggcctcg agcacgcgct
     2281 gggggtcgag atcggctttg cgcagtacat tggtctcgcc atagcggcct ggttcgagat
     2341 actcgccggc acgcacgtcc atcgacagcc gcacgatcgg atcatcctgc gcctggcggt
     2401 gcacttcggt gagcatggcg tccggctcgg cttcggtgaa aaagccgcct ccctgaatcg
     2461 ggggaagctg cgcgggatcg ccgagcacca gcaccggcac gccgaaggag agcaggtctc
     2521 ggccgagttc ggcatcgacc atcgagcact cgtcgatgat gatgagttcg gctttcgagg
     2581 cgggcgcttc gtcccacagg tcgaagcttg ggatttcctc gccgctctca cgcgcgcggt
     2641 agatcaggct gtgaatggtg gaggcgcctt tgcaaccttt gccgcgcatc accgaggcgg
     2701 ccttgccggt aaaggcggcg aacttcacct cgccgtcggc ttcgtcggcg atgtggcggg
     2761 cgagcgtggt tttgccggtg ccggcatatc cgaacaggcg gaagacctgc ggcgtgccgt
     2821 tcgagccggg cccggccttg acccaatcag acactgattt cagtgcgtta tcctggtggg
     2881 gagtgaaccg gggcatgtca tccaatccat aaagcaagca ccgttggagc gaaagaacaa
     2941 aatatgaact ttgtgttgac atcatcgact aggataatat tcatattgag tccggctcga
     3001 tcgaggggcg tagagaacga tcctgaaggc ggacgccact cggcgctcca tccccctcgg
     3061 tgacttgcga gggacaaggg tgaacgagcc tgcccggggc ctcagcaaag aatacgggcg
     3121 gcggagcgtt ggctagatgc cgggctgttt gacaagtgaa tcaggaaaag acccatgcac
     3181 gagcgcaaaa ttgccctgcc gatcctcaca ttgctctttg ccggttccca tgtttaaggt
     3241 cgcgaccttt ttttgcccgg aaccatgcat ggctcgtgac caggtcgaca tgacgcccat
     3301 cgagtcgcgt gacgagcttg tcacgtggct cgaggcgggc tgcaagccga aggcgcagtt
     3361 ccgcgtcggc acagagcacg agaaattcgc tttcacggtc gccgatcacc ggccggtacc
     3421 gtattccggc aagcgcagca tccatggctt gctcgacggc atgcatagtc tgctcggctg
     3481 ggagccgatc atggagggtg acaacatcat cggcatgttc gacgtgaccg ggggcggcgc
     3541 catctcgctg gagccgggcg gccagttcga actgtcgggc gcgccttacg acaacgtcca
     3601 tcagacggct gcggaactga tggcgcatct ggcgcaggtg cgcgaagtgg caaagcctct
     3661 gggcatcggc tttctgggca tcggcatgac gccgaactgg acgcgcgccg acatgccgaa
     3721 gatgccgaag ggccgctaca agatcatgac cgcctacatg ccgaaggtcg gcaagctcgg
     3781 cctcgacatg atgtaccgga cctgcacggt gcaggcgaac ctcgacttcg ccagcgaagc
     3841 cgacatggtg aagaagctgc gcgtgtcgct cgcgttgcag ccggtggcga ccgcgctgtt
     3901 cgccaattcg cccttcaccg aaggcaggcc gaacggcttc ctgtcaatgc gctcgcagat
     3961 ctggaccgac accgacaacc agcgcgcggg catgctgccc tgggccttcg agaacggaat
     4021 gggcttcgag cgctgggtcg attacgcgct cgacgtgccg atgtatttcg tcaagcgcgg
     4081 cgacaagtac atcgacgttg cgggcaagtc gttccgcgat ttctttgccg gcaagctgcc
     4141 ggaattaccg ggtgagcggc cgacgatttc cgactgggcc aatcacctga gcacgatctt
     4201 cccggaggtg cggctcaagc gttatctcga gatgcgcggc gccgatggcg ggccgtggcg
     4261 tcgcctgccg tcgctgtcgg cgttctgggt cggactgctt tacgacgacg acgtgctcga
     4321 agcctgctgg gacatgatca agccgtggac ggcagaggag cggcaaaagc tgcgcgacga
     4381 cgtgccgaag cgcggcttca aggcgcaaat tggcaaccgc gacgcgctct cgctggcgga
     4441 tgagctgctg cggctgtgcg cgcaaggcct ggcgcggcgc aggcggctcg accgcaatgg
     4501 ccgcgacgag acgcgctacc tgcggccgct ggaagagtcg atcgcgcgtg gcatcacgcc
     4561 ggccgaggaa ctgctcgcgc gcttccatgg cgaatggggc ggatcggtcg atccgatctt
     4621 cgaggaatac gcgtactgaa ggcgcgagct attcagccgc ctgcgctgca tccgaaaggt
     4681 tgtcgagcga cgagatgatg tgctcggcca gcgcatcggc gagcgccgag ctctcgtggg
     4741 cattgcgcac cagcccgatg cggcaactcg gcaggtcggg gaaaccgtcc gacgcggaca
     4801 gaacgcgcat
//
LOCUS       DASSRL010000559         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_574873, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000559 DASSRL010000000
VERSION     DASSRL010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574873"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFL49_11345"
     CDS             complement(<1..771)
                     /locus_tag="VFL49_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET7912619.1"
                     /translation="MIVLLLAGGVGGWAATTQIAGALIAQGSVVVDSNVKKVQHPTGG
                     VVGKLNVQDGDRVKAGDILVQLDDTITRANLAIVTKGLDELAARKARLEAERDGADTI
                     TFPRMLLAHADDASVSIALANERKLFELRRTARLGQKAQLRQRITQLQDEINGLNAQQ
                     AAKAREISLIGKELDGVRELWKNNLVQITRLTALERDAARLEGERAQLIAAVAQTKGK
                     ISETELQIIQIDQDLSSEVAKDMREVDAKYGEFVERKVA"
     gene            complement(894..2654)
                     /locus_tag="VFL49_11350"
     CDS             complement(894..2654)
                     /locus_tag="VFL49_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HET7912620.1"
                     /translation="MQRSQSDRSLPVRSELAAALAACRGAFYGTAVISGMSNVLMLTG
                     AMFMLEIYDRVLPSRSMPTLVGLLVLVAVLFTALGVLDAIRARILVRIGGALDETLSG
                     RVYDTLIRMPLRIGGLNDGTQPLRDLDAVRSYLSSLGPVALFDLPWIPLYLAICFAFH
                     PLIGITALAGAIILIALTLLTEILMRAPTVAATQAAIARNSLAEASRRNAEALIAMGM
                     AGRIAARWNEANRQYMHGQQEASDLGGGLGAVSKVFRMLLQSVVLAVGAYLVIQQEAS
                     AGIIIAGSILSARALAPVDLAIAHWKGFVNARQSWHRLNKLLTGAPVQPVPMQLQAPV
                     QRLAVEAVSATPPGVPKIVVQDITFELEAGQGLGIIGPSGSGKSSLARLLVGVWQPLR
                     GNVRLDGAALDQWSPDSLGRHIGYLPQDVELLAGTVAQNIARFELQADNEAVIAAAKA
                     VGVHDLIVRLPAGYDTQIGDQGSALSAGQAQRIALARAVYRDPFLVVLDEPNSNLDSD
                     GEEALTRAILGVRERNGIVVVVAHRPSAIAGVDRLMMMSQGRIQAIGPKDEVLAKVLQ
                     RAVSAPRPLKVVPDSGSASA"
BASE COUNT          505 a          919 c          965 g          528 t
ORIGIN      
        1 ggcgaccttg cgctcgacga actcgccgta tttggcgtcg acctcgcgca tgtccttggc
       61 gacctcgctg ctcaggtcct ggtcgatctg gatgatctgc agctcggtct cggaaatctt
      121 gcctttggtc tgcgcgactg cggcaatcag ttgtgcgcgc tcgccttcga gccgcgcggc
      181 atcgcgctcg agtgccgtca gccgcgtaat ctgcacgaga ttgttcttcc agagctcgcg
      241 gacgccgtcg agctccttgc cgatcaggct gatttcgcgg gccttggccg cctgttgagc
      301 attcagcccg ttgatttcgt cctgcagctg cgtgatccgc tgtctcaact gcgctttctg
      361 gccgagccgg gccgtgcggc gaagctcgaa cagcttacgc tcgttggcga gcgcaatgga
      421 aaccgacgcg tcgtcggcgt gagcgaggag catgcgcggg aatgtgatgg tgtcggcgcc
      481 gtcgcgctcg gcttcgaggc gcgccttgcg cgcggcgagc tcatcgagac ctttggtgac
      541 aatggcgaga ttcgcgcgcg tgatcgtatc gtcgagctgc acgagaatat cgccggcttt
      601 gacacgatcg ccgtcctgca cgttgagctt gccgacaacg ccgccggtcg ggtgctggac
      661 cttcttcacg ttcgagtcga cgacgacgga gccttgcgcg ataagcgcgc cggcgatctg
      721 ggtcgttgcc gcccatccgc cgacgccgcc ggcgagcagc agcacgatca tgccgccggc
      781 cagcaagtgg cggtggatgg aatagcgcga gcttcccggc gcagcggcag gcaacagttt
      841 cgtggcccat gtctcaaggc gctcgctgat gacatgaatg aaacttggga tcttcatgcg
      901 cttgcacttc cgctgtccgg cacgaccttg agcggccgtg gcgctgaaac cgcccgctgc
      961 agcaccttgg ccaggacctc gtctttggga ccgatggcct ggatacggcc ctggctcatc
     1021 atcataagcc ggtcgacgcc cgcaatcgcg ctcggacggt gggcgacgac gaccacgatg
     1081 ccgttgcgct cgcgcacgcc gaggatggcg cgcgtgagtg cttcctcgcc gtcggaatcg
     1141 agatttgaat tcggttcatc caacaccacc aggaacggat cgcggtaaac cgcgcgcgcg
     1201 agagcgatcc gttgcgcctg gccggcggaa agggccgaac cctggtcgcc gatctgcgtg
     1261 tcgtagccgg ccggcaaacg gacgatgagg tcatgcacgc cgacggcctt ggctgcggcg
     1321 atcacggctt cgttgtcggc ctgcagctcg aagcgggcga tgttctgcgc caccgtgccc
     1381 gcgagcagct ccacatcctg cggcaggtat ccgatatggc ggccgagcga atcgggcgac
     1441 cattggtcga gcgcggcgcc gtcgaggcgg acatttccgc gcagcggctg ccagacgccg
     1501 acgagcaggc gggcgagcga cgatttacca gagccgctcg gcccgatgat gccgagtccc
     1561 tgaccggcct cgagctcgaa tgtaatgtcc tgcacgacga ttttcggcac gccgggcggc
     1621 gtcgcgctga ctgcctcaac ggccagccgt tgtaccggcg cctgcaactg catcgggacc
     1681 ggctgcacgg gcgcgccggt gagcagcttg ttcaggcggt gccagctttg tcgcgcgttg
     1741 acgaagcctt tccagtgcgc gatcgcgaga tcgaccggtg ccaaagcgcg ggcgctgagg
     1801 atcgaaccgg caatgataat gcccgcgctc gcctcctgct ggatgacgag gtaggcgccg
     1861 accgcaagta cgacggactg cagcaacatc cggaaaacct tggaaactgc accgagcccg
     1921 ccgccgagat cgctcgcctc ctgctggccg tgcatgtact gcctgttcgc ctcgttccag
     1981 cgcgcggcaa tgcggccggc catgcccatg gcgataagcg cctcggcgtt gcgcctgctc
     2041 gcttccgcaa ggctgttgcg cgcgatagcg gcctgtgtcg ccgccacggt gggcgcgcgc
     2101 atgaggatct cggtgagcaa ggtcagcgcg atcagaatga tggcgccggc gagggccgtg
     2161 attccgatca gcgggtggaa tgcgaaacag atcgcgagat aaagaggaat ccacggaagg
     2221 tcgaaaagcg caaccgggcc gaggctcgag agataggatc gcacggcatc gaggtcgcgc
     2281 agcggctggg tgccgtcgtt cagcccgccg atccgcaacg gcattcgtat gagggtgtcg
     2341 tagacgcggc cgctgagcgt ttcgtcgagt gcgccgccga tgcgcacaag gatgcgggcg
     2401 cggatcgcgt ccaggacgcc gagggcggtg aacagtaccg cgacaaggac cagcagcccg
     2461 accagcgtcg gcatgctgcg gctcggcagc acgcggtcgt aaatttcaag catgaacatc
     2521 gcgccggtca gcatcagcac attgctcatg ccgctgatca cggccgtgcc gtagaacgca
     2581 ccgcggcagg cagccagcgc tgccgcgagt tcagagcgaa ccggtaggct tcgatccgat
     2641 tggctacgct gcatcaaacc agcacctgac ggcacccggc ccttgctttt cagggccacc
     2701 cgaatagccg aaaatccaag tccccggatt taaatatgac acgaatgccg gtttttgtcg
     2761 ccggaggcgc ggaggcagcg ttaatgaccg ttcagctggc ttttgtgcgg tttgttgcaa
     2821 cttcggctca aaaacatccc atggggccgg tcgacagccg cttggtatat agccgctagg
     2881 tatgatgaag gatgtcgctt ccgcccagat tgatgtt
//
LOCUS       DASSRL010000560         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1169985, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000560 DASSRL010000000
VERSION     DASSRL010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169985"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFL49_11355"
     CDS             complement(<1..486)
                     /locus_tag="VFL49_11355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7912621.1"
                     /translation="MAAFLPAAALADSCPGNPQALGTERVLAVSAKVTPRVGRKHFPS
                     TLPLNAKELVLTFDDGPWPGTTSKVLDALKGECVRATFFLLGRNAAAHPQLVRRALSE
                     GHTLGHHTYSHPLLNLMALPRAEAEIDRGIAADEIAVYGSRKPALVTPFFRFPGFASN
                     QA"
     gene            613..2016
                     /gene="mgtE"
                     /locus_tag="VFL49_11360"
     CDS             613..2016
                     /gene="mgtE"
                     /locus_tag="VFL49_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7912622.1"
                     /translation="MLDQQQTEATEIAQLPRNKDGSIRKTFVRSVARRIEAGDASGLR
                     AQVGDLHGADMGALIEALEPEQRPRLVALLGIDFDFAALTEVDDAVRDEILEELPPQA
                     VAKGVSELESDDAVAILEDLPKEEQAEILEQLPQPDRAALARSLDYPEESAGRRMQTE
                     FIAVPPAWNVGQAIDYMRETQELPDRFFELYVTDENRRFRGAVPLDRLLRSKRPVPIS
                     ELMEPERHRIRADEDQEEVARMFQRYDLVAAPVVDADDRLVGIITFDDVADVIEQEAE
                     EDIKALGGVRDEELSDSVWKIARGRFNWLLINLATAFLASSVLGLFEGQLQKMVALAV
                     LAPIVASQGGNATTQTMTVAVRALATRDLSDANAWRVITRELMVGLLNGVAFAVITGV
                     AAYVWFKVPGLGLVIGLAMICNLLAAALGGILIPLVLNRLRFDPAVASSPFVTTVTDV
                     VGFFSFLGIATLWFGLR"
     gene            2105..>2812
                     /locus_tag="VFL49_11365"
     CDS             2105..>2812
                     /locus_tag="VFL49_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912623.1"
                     /translation="MAGWERPVHEINAEPAGARPGDWLPAWARGRDAPSSEPPPPTPA
                     KWILSASRLIGILSFVAVASMLAIAATGFVLARYDDERLLIGQHAALRSVITEFRSTL
                     DHSAPVDPRLLRMAERIPGLQNLKFESSPARDREAHPVINSAGRITGFFTWDKTRPMS
                     RIMGRLAAVFAAVAVIVFGFAGLSLWQLKRARRELATSAMEAARAADEDKLTALPNHA
                     KTLELLDLSLAERADDEC"
BASE COUNT          500 a          846 c          931 g          535 t
ORIGIN      
        1 ggcctggttc gaggcaaagc cgggaaaacg gaagaacggc gtcaccaatg ccggtttgcg
       61 gctgccgtag acggcaatct cgtcggcggc aatgccgcgg tcgatctcgg cttccgctct
      121 cggcaaggcc atgaggttga gcagcgggtg agagtaggtg tgatggccga gggtatggcc
      181 ttcgctcaag gcgcgccgga cgagttgcgg atgcgcggcg gcattgcgcc ccagcaggaa
      241 aaatgtggcg cggacgcatt cgcccttgag cgcatccaag actttagacg tagtgcccgg
      301 ccacggaccg tcgtcgaagg tgagcaccag ttctttggca ttgaggggta gcgtcgacgg
      361 aaaatgcttg cgcccgacgc gcggggtgac cttggcgctc accgcgagaa cgcgctcggt
      421 ccccagcgcc tgcggattgc cgggacatga gtcggccaac gccgctgcgg ggaggaaggc
      481 agccatcaaa acgaaaataa aacgggtcat ggcttgtccg ttacacgaat ttgacacgct
      541 aatcatttgc gccgcgcgta gggaagggcg tacaagcgtc gcgctttaaa ttgattgtcg
      601 gcgattttcg tgatgctcga ccagcagcaa accgaggcga ccgagattgc gcaactgccg
      661 cgcaacaagg acggctcgat ccgcaagacc ttcgtcagaa gtgtcgcgcg cagaatcgag
      721 gcgggcgatg cctccggcct gagggcacag gtcggcgacc tgcacggcgc cgacatgggc
      781 gcgctgatcg aggcgctgga gccggagcaa cgcccgcggc ttgtcgcgct gctcggcatc
      841 gatttcgact ttgccgcgct caccgaggtc gacgacgcgg tgcgcgacga gatcctggag
      901 gaattgccgc cgcaggcggt agccaaaggc gtcagcgagc ttgagtccga cgatgccgtc
      961 gcgatcctcg aggatctgcc gaaagaggag caggcagaaa tcctcgagca gctgccgcag
     1021 cccgaccgcg cagcgctcgc gcgcagcctg gattatcctg aagagtcggc cggccggcgc
     1081 atgcagacgg aattcattgc cgttccgccg gcctggaatg tcggccaggc gatcgactac
     1141 atgcgcgaga cgcaggaatt gccggaccgg ttcttcgagc tctacgttac agacgagaat
     1201 cgccgcttcc ggggcgcggt cccgctcgac cggctgctgc gcagcaagcg tccggtgccg
     1261 atctccgagc ttatggagcc ggaacggcac cggatccgcg ccgatgagga tcaggaagag
     1321 gtcgcgcgca tgtttcagcg ctacgatctc gtggcggcac ctgtggtcga cgccgacgac
     1381 cggctggtcg gcatcatcac cttcgacgac gtcgccgacg tcatagagca ggaagcggaa
     1441 gaagacatca aggcgctggg cggtgtccgc gacgaagagc tgtcggattc ggtctggaag
     1501 atcgcgcgcg ggcgcttcaa ctggttgctg atcaatctgg ctaccgcgtt tctggcctcc
     1561 tcggtgctcg gcctgttcga agggcaattg cagaaaatgg tcgccctggc ggtcctggcg
     1621 cctatagtgg cgagccaggg cggcaatgcc acaacgcaga cgatgacggt tgccgtgcgg
     1681 gcgttggcga cgcgcgacct gagcgacgcc aatgcgtggc gcgtgatcac gcgtgagctg
     1741 atggtcggcc tgctaaacgg cgttgccttc gccgtcatca ccggcgtggc ggcctatgtc
     1801 tggttcaagg tgcctggtct ggggctggtg atcggtcttg ccatgatctg caatctgctt
     1861 gccgccgcgc ttggcggaat tctcattccg ctcgtgctga atcggctcag gttcgacccg
     1921 gcggtggcat caagtccctt cgtcaccacg gtgacggatg tggtcggttt tttctctttt
     1981 cttggtattg ccacgctgtg gttcggcctc cgatgagtcg ggtctccgac tcggttacat
     2041 cttgttcacc ttatttgctg accaacgctt aaagggcgca ccgtaaccct ataccccacg
     2101 ccgcatggca ggctgggagc gcccggtgca cgagatcaat gccgaaccag cgggcgcgcg
     2161 ccccggcgat tggcttccgg cctgggcgag aggtcgtgat gcgccttcgt ccgaacctcc
     2221 accgccgacg ccggcaaaat ggattctgtc ggcttcgcgg ctgatcggta ttctttcatt
     2281 tgtcgccgtt gcgagcatgc ttgcaattgc tgctaccggc tttgtgctgg cacgttatga
     2341 cgacgagcgg ctgttaattg gccagcacgc cgcgttgcgc agcgtgatta ccgaattccg
     2401 ttcgacgctc gatcattccg cccctgttga tccgcggctg ttgcgcatgg cggagcgcat
     2461 tcccggcctg caaaatctga aattcgagag cagtccggca cgcgaccgcg aagcacatcc
     2521 ggtcataaat tccgccgggc gcattaccgg atttttcacc tgggacaaaa cccgcccgat
     2581 gtcccggatc atgggacggc tggcggcggt ctttgccgca gttgccgtca tcgtctttgg
     2641 ctttgccggg ttgtcgctct ggcaactcaa gcgcgcgcgg cgcgagcttg cgacgagcgc
     2701 gatggaggcc gcccgcgcag cggacgaaga caagctgacg gctctgccaa accacgccaa
     2761 gacgctggag cttttggacc tttcgctcgc ggagcgagcc gatgacgagt gc
//
LOCUS       DASSRL010000561         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1274770, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000561 DASSRL010000000
VERSION     DASSRL010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274770"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VFL49_11370"
     CDS             complement(<1..1032)
                     /locus_tag="VFL49_11370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7912624.1"
                     /translation="MTLDVHTLFLVTIYVEGILGLLLLFVWAQNMALRAVCWWGFAHV
                     TRLASIALFGLYGSSPDLISIDLANALLFTAFAMTWTGARVFDGRPVEPVYLVTGAVL
                     WLLVCRLPVVTESNELRSLIVSGIITAYIWLTAYEFWRGRGEALVSRWPAIVMFFAHG
                     ALFLLRTPMIALLPSASTGGDLYGSVWMTVLSFEALLLTVSSAFLLLAMAKERTELRH
                     RKAAMVDPLTGIANRRAFLQDASMLAKLHVANPRPTAFLVIDLDHFKSINDRFGHALG
                     DRVLEIFTEAARKSMRGSDLVGRLGGEEFAAVLTDTSREKAVAVAERIRETFAQMAHE
                     VDSQPVCGTV"
     gene            1302..2342
                     /locus_tag="VFL49_11375"
     CDS             1302..2342
                     /locus_tag="VFL49_11375"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7912625.1"
                     /translation="MQPRDGIIDAIGHTPLIKLKRASEATGCTVLGKAEFMNPGGSVK
                     DRAGRQMILEAEKRGELRPGGLVVEATAGNTGIGLALVANARGYRTLIVIPETQSQEK
                     KDMLRLCGAELVEVPAAPFSNPNNYQHVGRRLAEGLKKTEKNGVIFADQWNNLDNRKA
                     HYVSTGPEIWEECGGKIDGFICAIGTGGTIAGASTYLREKKRDIMIGVADPRGAAMYH
                     LFKDGKATATEGGSITEGIGLGRVTPIIDGLKVDKAYLIPDEEALPLIFDLLEHEGLC
                     LGGSSGINIAGAIRLAKDLGPGKTIVTILADYGTRYQSKLFSPEFLRSKKLPVPDWLE
                     RKSNIKPPFEKT"
BASE COUNT          527 a          730 c          750 g          376 t
ORIGIN      
        1 gactgtcccg cataccggct ggctgtcgac ctcgtgggcc atctgggcaa aggtctcgcg
       61 aatccgttcg gctacggcca cggctttctc gcggctggta tcggtgagca ctgcggcgaa
      121 ttcttcgcca ccgagccggc cgacgagatc ggagccgcgc atggacttgc gcgccgcttc
      181 ggtgaagatt tccaggacac ggtcgccaag cgcatgaccg aagcggtcgt tgatcgactt
      241 gaagtgatcg aggtcgatga cgagaaacgc cgtcggccgc ggattggcga cgtgcagctt
      301 cgcgagcatc gacgcgtcct gaagaaacgc gcggcggttg gcgatgccgg tgagcggatc
      361 gaccatggcg gccttgcggt gtcgcagctc cgtgcgctcc ttcgccatcg ccagcagaag
      421 gaacgcgctg gaaacggtga gcagcaaggc ctcgaagctt agcaccgtca tccagacact
      481 gccgtacagg tcaccgccgg tcgacgccga gggcagcagc gcgatcatcg gcgtgcgcag
      541 caggaacagc gcgccgtgcg cgaaaaacat gacgatcgcc ggccaccggg agaccagcgc
      601 ctcgccgcgc ccgcgccaga attcgtaggc ggtgagccaa atataggccg tgatgatacc
      661 ggagacgatc agcgaccgga gttcgttact ctcagtcacc accggcaggc ggcagacgag
      721 cagccagaga acagcgccgg tgacaagata gacaggctcg accggacgac cgtcgaacac
      781 gcgcgcgcct gtccaggtca tcgcgaaggc ggtaaagagc aatgcgttgg ccagatcgat
      841 gctgatgaga tcgggcgacg atccataaag accgaagagc gcaatcgagg cgagccgcgt
      901 cacatgcgcg aatccccacc agcagacggc gcgcagcgcc atgttctgcg cccagacgaa
      961 gagaagcagc aggccaagga tgccctcgac gtagatggtc accagaaaaa gcgtatgaac
     1021 gtcgagcgtc attcgtcagc caacctgcag agttccgaaa tccggaacca gcgcccccgt
     1081 cgccacgaat agacctccgc ggggaaaaat ctgatgaatg gaggtaccca gaatgggcga
     1141 atacgccgct atagtttcga agttattgcg aatgcggcta caatcgccca aatcggcaga
     1201 attttccgct ttggcaggga tttgcgcgac gtttctccgg ccttgtggcg ttttggggcc
     1261 tccactaggg tcaaacgctc ccaccagccc acgggaccgg catgcaacct cgcgacggca
     1321 tcatcgacgc aatcgggcat acgccgctca tcaagctaaa gcgcgcatcg gaagccacgg
     1381 gttgcacggt cctgggcaag gccgagttca tgaaccccgg cggatcggtg aaggaccgtg
     1441 ccggccggca gatgatactg gaggcggaaa agcgcggaga gttgcgcccg ggcggcctgg
     1501 tggtcgaggc gaccgccggc aataccggta tcggactcgc actcgtcgcc aatgcgcggg
     1561 gataccgcac gctgatcgtc atcccggaga cgcaaagcca ggaaaaaaag gacatgctgc
     1621 gcctgtgcgg cgccgaactg gtcgaagtgc cggctgcccc cttctccaac ccgaacaact
     1681 accagcacgt cgggcggcgg ctcgccgaag ggctgaagaa aaccgaaaag aacggcgtca
     1741 tctttgccga ccagtggaac aatctcgaca accgcaaggc tcactacgtc tcgaccggac
     1801 cggagatctg ggaagagtgc ggcggcaaga ttgacggatt catctgcgcg atcggcaccg
     1861 gcggcacgat tgctggcgca tcgacatatc tgcgcgagaa gaaaagggac atcatgatcg
     1921 gcgttgccga tccacgcggc gcggcgatgt accatctctt caaggatggc aaggcgacgg
     1981 caaccgaagg tggctccatc accgagggca tcggtctcgg ccgcgtgacg ccgatcatcg
     2041 atgggctgaa ggtcgacaag gcctatctca ttccggacga agaggcgctt ccgctgatct
     2101 tcgatttgct cgaacacgaa ggtctctgcc tcggcggctc gtcaggcatc aatatcgccg
     2161 gcgccatccg gctggcaaag gacctgggtc cgggcaagac catcgtcacc atacttgcag
     2221 actacggcac gcgctatcag tcgaagctgt tcagcccgga attcctgcgc tccaagaaat
     2281 tgccggtccc cgactggctt gagcgcaagt cgaacatcaa gccccccttc gagaagacgt
     2341 gagtagccga tggcgaccga gtgtctgttc cgcgacgatt cct
//
LOCUS       DASSRL010000562         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1554574, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000562 DASSRL010000000
VERSION     DASSRL010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554574"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFL49_11380"
     CDS             <1..508
                     /locus_tag="VFL49_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7912626.1"
                     /translation="KRDLFRSPVGDRATWRPFCLRRAMNPQSEQSLGYHSVLIVEDDR
                     AVRESLKFSLVIEGFAVRVYADAEQLLAEPLLPKFDCLIVDQNMPGMNGLDLVSELRA
                     RNVDAPAILITSHPGRSVRDQAAAAGISIVEKPLLNSLIEHVRDTVSAAAEKQSNNAT
                     ANLSRKKT"
     gene            505..1332
                     /locus_tag="VFL49_11385"
     CDS             505..1332
                     /locus_tag="VFL49_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7912627.1"
                     /translation="MIKDVLVNLSTKSDRDEARDYAISVAREFSAHITGIAFDSGMGI
                     PGSVFDGGVASVVAAYRAENRKLALSAKDKFDIAAKDAGVMAEVHVVEAGVVDAGTIF
                     GKTARNYDLSIVGQARPDSEAMEELIVESALFQSGRPVLIVPYIQRLGIKFGRIMICW
                     DGSRNAARAVADAMPILKRAGAIEIISIQPQKRMSELAGTGIAKHLARHALKVDVKQL
                     VGTDVDVASTILSYASDSSADLIVMGGYGHSRLREFVLGGATRGIIENMTVPTLMAH"
     gene            complement(1400..>3679)
                     /locus_tag="VFL49_11390"
     CDS             complement(1400..>3679)
                     /locus_tag="VFL49_11390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7912628.1"
                     /translation="ASAPRAGDLAVISQSGAIAAGLIEWAAPRSVGFSAIVSIGDQLD
                     VDFGDLLDYFALDRSTRAILLYIESIKDARKFMSAARAAARTKPVIVVKAGRHVRGAA
                     AATTHTGALAGADSVYDAAFRRAGLLRVSDLDELFAATETLGHVKCLSGKRLAILTNG
                     GGIGVLAVDRLMDLEENRDLLAAIGPRAMARLDAALPPIWSRANPVDIAGDADAARYA
                     AALEALLADDENDAVMVMNVATALASPLDAAAAVAETVRAHKSNAVFPKPVFAVWVGD
                     RDEASTRFEAAGIPHYQSESSAVAGYGHLVRHREALEMLMQTPPSLPEDFVPDTATAR
                     RVVAGVLAGRRAWLDPIEAEQLLRAYSIPIAPAIPASDPEAAVRAAMPFLNNNQPVAL
                     KVHSPDIIHKSDIGGVRLNLASAPAVRQAAADMLDHVHAAKPGARLSGVTVHPMIIRP
                     LARELIAGVADDPTFGPVVVFGRGGTAVEVINDKALALPPLDLKLAQELIARTRVSRV
                     LKAYRDVPAADEVAVALVLVKLAQLVADVPEIREVDLNPLLSDDSGVIAVDARIAVAP
                     LEQASRRFPGHPRFAIRPYPKEWERSCQLPNGTAIFVRPVRPEDERLYASFFARVTAD
                     DLRLRFFTLIKEFSHAFIARMTQLDYARAMAFVAIDQASGDLLGVARLHANADYDRAE
                     YAVLVRSDKKGLGLGWLLMQTLLEYARREGLRRIEGQVLKENTTMLEMCRELGFSVAG
                     DPYDPGSYLVGLELEPAGR"
BASE COUNT          791 a         1080 c         1082 g          726 t
ORIGIN      
        1 gaaaagagac ctattcaggt ctcctgtcgg cgaccgggcc acatggcggc ccttttgctt
       61 acgccgagca atgaacccgc aatctgagca aagccttgga tatcattcgg tcctcatcgt
      121 ggaggacgat cgcgcggtcc gtgagtcatt gaagttttct ctcgtgattg agggatttgc
      181 ggttcgcgtc tacgcggatg cggagcaact acttgcagaa ccgctccttc cgaaattcga
      241 ctgtctgatt gtcgaccaga acatgccggg aatgaacggt ctcgatctgg tttcagagtt
      301 gcgcgctcga aacgttgacg ctccagcgat acttattacc agccatcccg gtcgatctgt
      361 gcgggatcag gctgcggccg ccggtatatc cattgtagaa aaacctctgc tgaattctct
      421 catcgagcac gttcgggaca ctgtttcggc ggcggccgag aaacagtcga acaatgcgac
      481 agccaactta tcgaggaaaa agacatgata aaggatgtgc tcgttaacct gagtactaaa
      541 tctgatcgcg acgaggcacg cgattacgcg atttcggtag cacgcgaatt cagtgcgcac
      601 ataaccggaa tcgcttttga cagcgggatg ggcattccag ggtcggtctt tgatggaggc
      661 gttgcctcgg ttgtcgcagc ctatcgcgcg gaaaacagaa aactcgctct ctcagccaag
      721 gacaaatttg atatcgcagc aaaagacgcc ggggttatgg cggaagtcca tgtcgttgag
      781 gctggagttg tggatgccgg caccatattt ggcaagaccg cacgcaacta cgatctgtca
      841 atcgttggtc aggcacggcc agatagcgag gcaatggaag agttgattgt cgaaagcgct
      901 ctgttccaat caggtcggcc ggtcctgatc gttccctata ttcagcggct cggaatcaag
      961 tttggtcgta tcatgatttg ttgggacggg agccgcaacg ccgcccgagc cgtcgcggac
     1021 gctatgccga tcctcaaacg cgctggagcg atagagatta tctcaatcca gccgcaaaag
     1081 cgcatgagtg agctcgccgg caccggcatt gccaaacatc tggcgcggca tgccctcaag
     1141 gtcgatgtca agcagttggt tgggactgat gttgatgtgg cgagtacgat cctttcctac
     1201 gctagcgact cctcagcaga cctgattgtt atgggtggct atggccactc ccgcttgcgc
     1261 gagttcgtcc tcggaggcgc aactcgcgga atcatcgaga acatgaccgt gccaacactc
     1321 atggcgcatt gatgcacgcg aaatgagagg ggctgatttg tttgctatca agcaatctca
     1381 agcgaatcgg atatcacgct cagcggccag cgggctccaa ttccagacca acgaggtagc
     1441 tgccaggatc atatggatcg ccggcgaccg aaaagccgag ctcccgacac atctccagca
     1501 ttgtcgtgtt ttctttcagc acctgacctt cgatgcgacg taatccctcc ctgcgggcgt
     1561 attcgagaag tgtctgcatg agtagccacc cgaggcccaa gcctttcttg tccgaccgga
     1621 ccaagacagc atactcggcc ctgtcatagt cggcattggc gtgcagcctc gcaacgccga
     1681 gcaaatcgcc gcttgcctgg tcgatcgcca caaaagccat ggcccgcgcg tagtcgagct
     1741 gggtcatacg ggcaatgaag gcatggctga attctttgat cagggtaaag aatcgaagac
     1801 gcaagtcatc cgccgtgacc cgagcaaaga aggaagcgta caaccgctca tcctccggac
     1861 gaacggggcg cacgaatatc gcagtgccat tcggcaactg gcatgaccgc tcccattcct
     1921 ttggataagg ccgaatagcg aagcgtgggt gcccggggaa ccgacgactg gcctgttcaa
     1981 gcggcgcgac tgcgatgcgt gcatcgacgg caataacccc ggagtcgtcg ctcagtaacg
     2041 ggttgaggtc tacttccctg atctcaggca cgtcggctac gagctgagca agcttgacga
     2101 gtacgagcgc gaccgcgacc tcatccgccg ctggcacgtc tcgataggct ttgagcacac
     2161 gcgagacgcg tgttcgcgca atcaactctt gcgcgagctt gaggtcgagc ggcggaagcg
     2221 ccagcgcctt gtcattgatg acttcgacag cggtccctcc ccgaccgaac acgaccactg
     2281 gtccaaaagt gggatcgtcg gctacgccgg caatcagctc acgcgccaac ggacgaatga
     2341 tcatcggatg tacggtaacg ccggagagcc gcgcgccggg tttagctgcg tgcacatgat
     2401 caagcatgtc agccgccgcc tgtcgcacgg ccggcgcgct agccagattg agccgcacgc
     2461 cgccaatatc cgatttatgg attatgtcgg gcgagtggac cttcagggca acaggttgat
     2521 tgttgttgag gaacggcatg gccgcacgca cggccgcctc cgggtcggac gcgggaatgg
     2581 ccggtgcgat cgggatggaa tatgcacgaa gcagctgttc tgcctcgatc gggtccagcc
     2641 atgctcttcg ccccgccagc acaccggcta caacgcggcg ggccgtcgcg gtatccggta
     2701 caaaatcctc cggaagactt ggcggcgttt gcattagcat ctcgagcgct tcgcggtgcc
     2761 gcaccaggtg gccatagcct gcgactgcac tgctttcgga ctggtagtgc ggaattcctg
     2821 cggcctcgaa tcttgttgac gcttcatcac ggtcgcccac ccaaacggcg aaaacgggtt
     2881 tgggaaaaac tgcgttgctc ttgtgagcac gaaccgtttc cgcgacggcc gccgcggcat
     2941 ccaatggcga agcaagtgct gttgccacgt tcatgaccat gacagcatcg ttctcatcgt
     3001 cggctaaaag ggcctcaaga gccgcggcat agcgcgctgc gtcggcatct cccgcgatgt
     3061 cgaccggatt ggccctggac cagatgggcg gcagcgccgc atcgagcctg gccatggccc
     3121 ttggccctat tgccgcgagc agatcgcgat tttcttcaag atccatgagc cgatcgaccg
     3181 cgaggacgcc gataccgcca ccgttcgtca aaatcgcaag tcttttcccg gaaaggcatt
     3241 tgacgtggcc cagggtttcg gtggctgcaa atagctcgtc caggtccgaa acgcgcagca
     3301 agcccgcccg acggaacgcg gcgtcgtaga cgctgtcagc gccagccaag gcgccggtat
     3361 gagtcgtcgc ggcagccgcg ccccgtacat ggcggccggc tttgaccaca atgacaggct
     3421 tggtccgcgc cgcggcgcga gcggcggaca taaacttacg ggcgtccttg atcgattcga
     3481 tgtaaagcag aattgcccgc gtgcttcgat cgagtgcgaa ataatcaagc aggtctccaa
     3541 aatcgacgtc gagctgatcc ccgatcgaca cgatggccga aaaccccacc gaccgtggtg
     3601 cggcccactc tataagaccc gcggcaatcg cgccggactg tgaaatcacg gccaagtcac
     3661 ccgcacgagg cgcgctggc
//
LOCUS       DASSRL010000563         6660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_172086, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000563 DASSRL010000000
VERSION     DASSRL010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172086"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFL49_11395"
     CDS             complement(<1..261)
                     /locus_tag="VFL49_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7912629.1"
                     /translation="MTAEKSKALAHIRVLDVSRVRAGPTCCRILGDFGADIIKIEAPP
                     GVDPNEGISGARDGYDMINLHRNKRSLTLNLKERSGHEIFMRL"
     gene            complement(258..1607)
                     /locus_tag="VFL49_11400"
     CDS             complement(258..1607)
                     /locus_tag="VFL49_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7912630.1"
                     /translation="MGYPIGKTVTKRFTDWDSLDAAERARISAQSGEFARALDGPLKA
                     YVAFEDATTPVQGVLDGMPYASKDMFVSKTRKPHGGLAQPLPMESSQQAAVLDLLDRA
                     GANRIGTTAMTELAYEPSGYNAAHGAPKNPWNFDYVTGGSSSGSAVAVASGSVVFALG
                     SDTGGSLRIPAHCCGVTSWKPTYGVVSVTGAMPLSPSLDTVGLLARSASDLQEPAHIL
                     YPAHDADPIRKVTVVEDVLARAEVPIANACGNAIEVIGGCGVELARTIGIAAIEALDP
                     HVFTIMQAEAARAHRALMDTGALDVTLTKRLRKGLEIDDATLAASIAARSQLVADFLS
                     SVFQNAQAIALPALTTRTPAVRECDPRSASFNAKTLYQLSRWTRFVNMLGFPAVTIPA
                     GFDDRGLPVALQIVGRPGADHALIALAAAVQKRSDWHARIPAAIHEQVISSYQGRFV"
     gene            complement(1580..2200)
                     /gene="leuD"
                     /locus_tag="VFL49_11405"
     CDS             complement(1580..2200)
                     /gene="leuD"
                     /locus_tag="VFL49_11405"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET7912631.1"
                     /translation="MEKFVRLTATACPLSLPNLNTDQILPARYLKWPRSKGLGTVLFD
                     DVRLDAKGQPREDFAFNKDVYRGAKVLLAGRNFGGGSSREAAVYALYDHGYRCVLAPS
                     FGDIFAQNAVKNGLLTAVAAEADIETLATLVREKPAQPVIIDLEKQTVSGGDISFAFT
                     IDPISRNQLLNGWDDVDLTQSYREQIAAFKTKDKTVRPWATPSVKP"
     gene            complement(2209..3621)
                     /gene="leuC"
                     /locus_tag="VFL49_11410"
     CDS             complement(2209..3621)
                     /gene="leuC"
                     /locus_tag="VFL49_11410"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET7912632.1"
                     /translation="MSEPRTLVDKLWSAHEIARREDGAALLWVDRHYVHEGSFHAFSQ
                     LKARGRTVAEPKLTFGVADHYVPTRGRDGGIANPEIAAMVRNLEANTTENHITLFGLR
                     DPRQGIVHVVGPEQGLTLPGLLIVCGDSHTSTHGAFGAYAFGIGASEVAHVLMTQTLW
                     QRKPKRMRITVDGTLGAGVAAKDIALAIIAHIRADGAQGHAIEFAGSAIRSLSIEGRL
                     TLCNLSIEAGGRCGMVAPDATTFAYLKGRPFAPKGSDFDRAVEAWSALTSDAGARFDR
                     EVSLDANEIAPIVTWGTTPDDALPVDGRVPDPAREADATRAKYLREALDYMGLAPGTK
                     LTDIAVDRVFIGSCTNSRIEDLRAAAAVLSGRVSKVPGLVSPGSSSVKRQAEEEGLDR
                     IFRDAGLEWADSGCSMCVGINGDLVLPGERCASTTNRNFRGRQGPGARTHLMSPAMVA
                     AAAVSGHLADVRPLLAGRKA"
     gene            3746..4591
                     /locus_tag="VFL49_11415"
     CDS             3746..4591
                     /locus_tag="VFL49_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7912633.1"
                     /translation="MRHWLGEAKAKRVYLVLRDRILSGAVGFGAKLPTENELARYHGV
                     SRVTVRRALGELARERLIERRPSAGTRVIYRPSPAPMTADISGVLANLADMGRRTTVK
                     LLSFDYVPAQGAVAEALGARADQLLQRAVRVRSIDGQPFSYLVTHVPESVSVTFTEDE
                     LAHRPLLELLERAGVKVERARQRISAGLATPQVAKALGLHTGSPLIELVRIVYDQAGR
                     GVEYLHALYRPDRYSFEFDLMRSGVNNARSWAPVVGNRVKRPSPTIAKGSNHKTKPLD
                     LAPRR"
     gene            4611..5822
                     /locus_tag="VFL49_11420"
     CDS             4611..5822
                     /locus_tag="VFL49_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912634.1"
                     /translation="MSNLKSVSRRRFLASSALGVAVVSMPGILRAQGAPVKLGVLHPV
                     TGALSYSGQQGRLGATLAIEEINAAGGVKGLGKIDPVIGDAQSTPEGGVAEVEKMNSA
                     GVACIVGGYASSICLATTQAAARYELPYVVDVGVADGIVTRGLKNTFRFGPGFGVIAK
                     TALANLNTINEQAGKPAKTVMIVHEDSLFGSGLAKLLNAQLPQQGFQILETIAHPTPT
                     RDFNNVVLKIKAQNPDLVIPANYYNEYVLLARTMQQQHVRPKGIYSVLGGAASSYKFV
                     KEFPEAARYIMDCNHWFDPRNPKAIALKKKVEDKGQFYTYEVYMNYSCILLVADALQR
                     AASADRAKLTEALASSTFAGHVMPYGPTKFVNGQNEGAAPVNTQVLDNDIKVILPATF
                     ANAKPVFPMPA"
     gene            5886..>6660
                     /locus_tag="VFL49_11425"
     CDS             5886..>6660
                     /locus_tag="VFL49_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912635.1"
                     /translation="MFSAAILIPAVLNGLLMGAIYALIALGLTLIYGVLHIINFAHGA
                     LLSAALFAAFFAFKLFGLDPYLALFLLAPLFFVLGYGLQRFLIGPASHGDDRNMLLVT
                     LGIAVIIENALLYSFRGDTRTIDVPYAFQTFNLGVTYLAVPRVVGFVAVFVVALALWL
                     LMTFTDVGRAIRAVAKEKLGAQLVGIDVAHVYAMTFGLGTACVAIAACLLIPTYYVNP
                     GVGNAFVLVAFTIVVLGGMGSVVGALIGGLFIGVIESLSG"
BASE COUNT         1154 a         2098 c         2179 g         1229 t
ORIGIN      
        1 cagccgcatg aagatctcgt gcccggatcg ctccttcaga ttgagcgtga gcgagcgctt
       61 gttgcggtgg agattgatca tgtcgtagcc atcgcgcgcg ccgctgatgc cttcgttcgg
      121 gtcgacgccc ggcggtgctt cgatcttgat gatgtcggcg ccgaagtcgc cgaggatgcg
      181 gcagcaggtg gggccggcac gcacgcgcga cacatcgagc acgcggatat gcgccagtgc
      241 tttcgatttc tcggcggtca tacgaaccgt ccctggtagg acgatatcac ttgctcgtgg
      301 atcgcggcgg gaatccgcgc atgccagtcg ctgcgtttct gcacggctgc ggccagcgcg
      361 atcagcgcgt ggtccgcgcc ggggcggccg acgatctgca atgcaaccgg aaggccgcgg
      421 tcatcgaacc cggccgggat ggtgactgcg gggaaaccga gcatgttaac gaaacgggtc
      481 cagcggctga gctggtagag cgttttcgcg ttgaacgaag ccgagcgcgg atcgcattcg
      541 cgcaccgccg gcgtccgtgt cgtcagcgcc ggcagagcaa tcgcctgcgc gttctggaat
      601 acgctggaga gaaaatcggc cacaagttgc gagcgcgccg cgatacttgc ggcgagcgtc
      661 gcgtcgtcga tctcgaggcc cttgcgcagg cgttttgtca gcgtcacgtc gagcgcgccg
      721 gtgtccatca gcgcgcgatg cgcgcgcgcc gcttccgcct gcatgatcgt gaagacatgc
      781 gggtcgagcg cctcgatcgc ggcaatgccg atggtgcggg caagctcgac gccgcagccc
      841 ccgatgacct cgatcgcatt gccgcatgca ttcgcgatcg gcacctcggc gcgggcgagc
      901 acgtcctcga ccacggtgac cttgcgaatg gggtcggcat cgtgtgcggg atacaaaatg
      961 tgcgcgggct cctgcagatc agaggcgctg cgcgcaagga ggccgaccgt gtcgaggctc
     1021 ggggagaggg gcatcgcgcc ggtaaccgac acaacgccat aagtcggctt ccatgacgtg
     1081 acgccgcagc aatgcgcagg aatgcgcagg gagccgccag tgtccgaccc gagtgcgaaa
     1141 acgacagagc cgctggcaac agcgaccgcg gagcctgacg acgagccgcc ggtaacataa
     1201 tcgaagttcc agggattttt tggcgcaccg tgcgcggcgt tgtagccgga cggctcatag
     1261 gcgagctcag tcattgccgt agtgccgata cggttcgcgc cggcgcggtc gagcaaatcg
     1321 agcacggcgg cctgctgtga cgactccatg ggaagcggct gcgccagccc gccatgcggc
     1381 ttacgtgttt tggaaacgaa catgtctttc gacgcataag gcattccgtc gaggacgcct
     1441 tgaactggtg tggtggcgtc ttcgaacgcg acgtaggcct tcaatggccc atcgagagcg
     1501 cgcgcgaact cgccggactg cgccgaaatc cgtgcacgct cggcggcatc gagtgaatcc
     1561 cagtcggtaa accgtttggt cacggtttta ccgatggggt agcccatggc ctgaccgtct
     1621 tgtctttggt cttgaaagcg gcgatctgct cgcgatagct ctgcgtcagg tcgacgtcgt
     1681 cccaaccatt cagcaattga ttgcggctga tcgggtcgat tgtgaacgca aacgaaatat
     1741 caccaccgct gaccgtctgc ttttccaggt cgatgatgac cggctgcgcc ggtttctcgc
     1801 gcacgagcgt ggcaagagtc tcaatgtccg cttcggcggc aacggcggtg agcaggccat
     1861 tcttcaccgc attctgcgcg aagatatcgc cgaaggacgg cgcgagaaca cagcggtagc
     1921 cgtggtcgta gagcgcgtag accgcagcct cgcgcgagga gccgccgccg aaattgcgtc
     1981 ccgccagtag cacctttgct ccgcgataga catccttgtt gaatgcgaag tcctcgcgcg
     2041 gttggccctt ggcatcgagc cgcacgtcgt cgaacaaaac cgtgccgagc cctttcgacc
     2101 ttggccattt cagatagcgt gccggcagaa tctggtcggt attcaggttc ggcagcgaca
     2161 gcgggcaggc ggttgcggtg aggcgaacga atttctccat cggcgcctct atgctttgcg
     2221 tccggcaagc aacggccgga catcggcgag atggccgcta acggccgccg cagccaccat
     2281 ggctggcgac atcagatgcg tccgcgcacc aggtccctgc cggccgcgaa agttgcgatt
     2341 ggtggtcgag gcgcagcgtt cgcccggcag caccaggtcg ccattgatgc cgacgcacat
     2401 cgagcagccc gaatcggccc actcgaggcc ggcgtcgcgg aaaatcctgt cgaggccttc
     2461 ttcctcggcc tgccgtttca ccgacgacga gcccggcgag accaggccgg gtaccttact
     2521 gacgcggccg ctgagcacgg cggcggccgc gcgtagatcc tcgatgcgcg aattggtgca
     2581 tgagccgatg aagacccggt cgaccgcaat gtcagtgagc ttcgttcccg gcgcaagccc
     2641 catatagtcg agcgcctcgc gcagatattt ggcgcgcgtc gcgtcggcct cgcgcgccgg
     2701 atcgggcaca cggccgtcga ccggcagggc atcatccggc gtcgtgcccc aggtgacgat
     2761 cggcgcgatc tcgttcgcat cgagcgagac ctcgcgatcg aagcgagcgc ccgcatccga
     2821 ggtgagcgcg gaccacgctt cgaccgcgcg atcgaaatcg gagcctttcg gcgcgaaggg
     2881 ccggcctttc aggtaggcga aggtggtcgc atccggcgcc accatgccgc aacggccccc
     2941 ggcctcgatt gacagattgc agagtgtcag ccggccttcg atcgacagcg agcggatggc
     3001 gctgccggca aattcgatgg cgtggccctg tgcgccgtcg gcccgaatgt gcgcgatgat
     3061 cgcgagcgcg atatctttcg ccgcgactcc ggctccaagt gtgccgtcaa cggtgatgcg
     3121 catacgcttc ggcttcctct gccacagcgt ctgcgtcatc aatacgtgcg cgacctcgga
     3181 cgcgccgatt ccgaaggcat aagcaccgaa cgcaccgtgc gtcgatgtat ggctgtcgcc
     3241 gcaaacgatc agcagcccgg gcagtgtcag tccctgttcg gggccgacca catgcacgat
     3301 gccctggcgt ggatcacgca ggccgaacag cgtgatgtgg ttttcggtcg tgttcgcctc
     3361 gaggttgcgc accatggcgg cgatctcggg attggcaatt ccgccgtcgc ggccgcgcgt
     3421 cggcacgtag tggtcggcga caccgaaggt gagtttcggc tcggccacgg ttcgaccgcg
     3481 cgccttcagc tgcgagaacg cgtggaacga gccctcgtgc acataatggc ggtcgaccca
     3541 gagcaaagcc gcgccgtctt cgcggcgcgc aatttcgtgc gcagaccaca atttatcgac
     3601 gagagtgcgg ggctcgctca tgctgtgccg gctgctgtgc ctcacccctg cgggcttgtc
     3661 gaggcgccac aatattgtat gctaaaaaat accaagcaga ataccaagta aagctctgat
     3721 cggcgttggg taagggggag cggcgttgcg ccattggctc ggcgaagcga aggcgaaacg
     3781 ggtctatctc gttttgcgcg accgcatcct gagcggggcg gtcggcttcg gcgcgaagct
     3841 tcccaccgaa aacgagcttg cccgttacca cggcgtctcg cgggtgaccg tgcggcgcgc
     3901 gctcggcgag ctcgcgcgcg agcggctgat cgagcgccgg ccgagtgccg gcacgcgcgt
     3961 catctaccgg ccgtcgccgg cgccgatgac cgcggatatc tccggcgtgc tcgcgaacct
     4021 cgccgacatg gggcggcgca ccaccgtcaa acttctctct ttcgactatg tgccggcgca
     4081 gggcgcggtg gccgaggcgc ttggagcgag ggccgatcag ctgctgcagc gcgccgtgcg
     4141 cgtgcgctcg atcgacggcc agccgttttc ctatctcgtt acacacgttc ccgagagcgt
     4201 ttcggtgacc ttcaccgagg acgagcttgc gcaccggcca ctgctcgaac tgctcgagcg
     4261 cgccggggtg aaggtcgagc gcgcccgcca gcgcatcagc gccgggctgg caaccccgca
     4321 ggttgccaag gcgctcgggc tgcacaccgg ctcgccgctg atcgaactcg ttcgtattgt
     4381 ctacgaccag gcgggccgcg gcgtcgaata cctgcacgcg ctctaccggc ccgaccgtta
     4441 cagcttcgaa ttcgatctca tgcgctccgg cgtgaacaac gcgcgcagct gggcaccggt
     4501 ggtcggcaac agagtcaaac gtccgtcccc gaccattgca aaaggttcaa accacaagac
     4561 aaagccgctg gatcttgcgc cacggcgatg aaacagggag gagagtcgtc atgtcgaacc
     4621 tgaaatcggt cagccgccgg cgctttctgg cgagcagcgc gctcggcgtt gcggtcgtat
     4681 cgatgccggg aatcctgcgc gcgcagggcg cacccgtgaa gctcggcgtg ctgcatccgg
     4741 tcaccggcgc gctgtcctat tcgggacagc agggccggct cggcgcgacg ctcgcgatcg
     4801 aggagattaa cgccgccggc ggcgtcaagg gcctgggcaa gatcgatccc gtgatcggcg
     4861 atgcacaatc gactcctgag ggcggggttg ccgaagtcga aaagatgaat tcggccggcg
     4921 ttgcctgcat cgtcggcggc tatgccagca gcatctgcct tgccacaacg caggcggcgg
     4981 cgcgctatga gttgccttac gtcgtcgatg ttggtgtcgc cgacggcatt gtgacgcgcg
     5041 ggttgaagaa cacgttccgt ttcggacccg gcttcggcgt catcgcgaag acggcgcttg
     5101 ccaacctcaa tacgatcaat gagcaggcgg ggaagccggc caagacggtc atgatcgtgc
     5161 acgaggattc gctgttcggc tcgggtcttg ccaagctgct caacgcgcag ctgccgcagc
     5221 agggtttcca gatcctcgag acgatcgcgc acccgacgcc gacgcgcgac ttcaacaacg
     5281 tcgtgctcaa gatcaaggcg cagaacccgg atctggtgat ccccgccaac tactacaatg
     5341 agtacgtatt gctcgcgcgc accatgcagc agcagcacgt gaggccgaag ggtatctatt
     5401 cggtgctggg aggcgcggcc tcttcctata agttcgtcaa ggaattcccc gaagccgcca
     5461 gatacatcat ggattgcaat cactggtttg acccgcgcaa cccgaaggcg attgcgctga
     5521 agaaaaaggt cgaggacaag ggccagttct acacttacga agtctacatg aactacagct
     5581 gcatcctgct cgttgccgat gcgctgcagc gagcggcctc tgcggaccgc gcgaaactga
     5641 ccgaggcgtt ggcgagctcg acctttgccg ggcacgtgat gccctatgga ccgaccaagt
     5701 tcgtcaacgg gcagaacgag ggtgcggcgc cggtcaatac ccaggtgctc gacaatgaca
     5761 tcaaggtgat cttgccggcc acattcgcca acgcgaagcc ggtattcccg atgcccgctt
     5821 aacagcatac ggctttgcgg gcggcgcctt gtgcgccgtc cgcgggccgc cgggcgggac
     5881 gtgtaatgtt ttcggctgcc atcctgatcc ccgccgtgct caacggcctg ctgatgggcg
     5941 cgatctatgc gctgatcgcg ctcgggctga cgctgatcta cggcgtgctg cacatcatca
     6001 atttcgcgca cggcgcgctg ctgagcgcgg cgctgtttgc cgcattcttt gcgttcaagc
     6061 tgttcgggct cgacccctac ctggcgctgt ttctgctggc gccgcttttc ttcgtgcttg
     6121 gttacggatt gcagcggttc ctgatcggtc cggcctcgca tggcgatgac cgcaatatgc
     6181 tgctggtgac gctcggcatt gccgtgatca tcgagaatgc cttgctctac agcttccgcg
     6241 gcgatacccg aacgatcgac gtaccctacg ccttccagac cttcaatctc ggggtgactt
     6301 acctggcggt gccgcgcgtc gtcgggtttg ttgcggtctt cgtggtggcg ctggcgcttt
     6361 ggctgctgat gacctttacc gatgtcggcc gcgccatccg cgccgtcgcc aaggaaaagc
     6421 tcggcgccca attggttggg atcgatgtcg cgcacgtcta cgcgatgacc ttcggcctcg
     6481 gcaccgcctg cgtggcgatc gcggcgtgcc tgctgatccc gacctactac gtcaatccgg
     6541 gcgtgggtaa cgccttcgtg ctggtggcct tcaccatcgt cgtgctcggc ggcatgggtt
     6601 cggtggtggg cgcgctgatc ggcggattgt tcattggcgt gatcgagagc ctatcggggc
//
LOCUS       DASSRL010000564         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1449874, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000564 DASSRL010000000
VERSION     DASSRL010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449874"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFL49_11430"
     CDS             <1..253
                     /locus_tag="VFL49_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET7912636.1"
                     /translation="GNPIFDSTGMGLAEGGDLYQQAVAIVKRDRKASTSYIQRRLQIG
                     YNRAATIMERMEEEGIVGQPNHAGKREILIPENPADDRY"
     gene            373..1203
                     /locus_tag="VFL49_11435"
     CDS             373..1203
                     /locus_tag="VFL49_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET7912637.1"
                     /translation="MAAPVRARTHAKLGCAALLACLAVPALAQTVPLPVPRPSVLQTH
                     AGTPSSSTPTVKPAVPNQALGARAQAPQPAPSQNPISNPFAALLGKAGGGGKLTPEQR
                     AIVERVNSYLSGVQTLTGNFVQVGPDGSRTQGDFYISKPGRVRFEYDDPSPIELIADG
                     SSVVVRDRRLATQDVYPLSQTPLRFLLAERVDLTKDTNLVSVYADDVFVTVVLEEKNG
                     IVGTSRLMIMFSAKDMQLKQWTVTDPQGYDTTVAVYNLDTSKRPDPALFKIDYTRYHN
                     "
     gene            1317..2135
                     /locus_tag="VFL49_11440"
     CDS             1317..2135
                     /locus_tag="VFL49_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7912638.1"
                     /translation="MSLKVTTWNINSVRLRINLVASFLKSTQPDVLCLQETKCPDDVF
                     PLKRFRRLGYEHAALSGQKGYHGVAVLSRFPFEQVETQSFCGKADCRHISVVLGERAE
                     LKGPVTLHNFYVPAGGDEPDPAVNPKFKHKLDFLDEMRGHAGLRPEKSQRAILVGDLN
                     VAPLEHDVWSHKQMIKVVSHTPIECEKLVAARTSGGWIDSMRVLTPEPEKLYTWWSYR
                     AMDNWRAADRGRRLDHIWTSDALADRVSTIKIGKSYRGAKRPSDHVPVTATIEL"
     gene            complement(2155..2619)
                     /locus_tag="VFL49_11445"
     CDS             complement(2155..2619)
                     /locus_tag="VFL49_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7912639.1"
                     /translation="MSLEEDVAFFEQVPPLAALGKQALRILAIGAETRHLHSGAVLFY
                     AGELADGGYVIQEGSLLLEPGTLSEGKEITVGRGTLIGELALLTNTVCQATAIAKEPT
                     VVVRISRNLFRKMLEGYPGAAEKMRESMTERLQSWSRELAGVKRALEARDKT"
     gene            complement(2645..>2794)
                     /locus_tag="VFL49_11450"
     CDS             complement(2645..>2794)
                     /locus_tag="VFL49_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7912640.1"
                     /translation="LLQEVWGYNSGVTTHTLETHIYRLRQKVEKDAAAPAILVTEAGG
                     YKLVP"
BASE COUNT          585 a          851 c          797 g          561 t
ORIGIN      
        1 tgggaacccg atttttgatt cgacgggcat gggactcgcc gaaggcggcg atctttatca
       61 acaggcggtc gcgatcgtga agcgcgaccg caaggcttcg acctcctata tccagcgtcg
      121 cctccagatc ggttacaacc gcgccgccac tatcatggag cgtatggagg aagagggcat
      181 cgttgggcag cccaaccatg ccggcaaacg cgagatcctg atcccggaga atccggccga
      241 cgaccgctat tgagaatcgc ccggttcgcc ggtaagccgt tttaccgttg gaacgactgg
      301 gtcgcgaaat cgccacagcc agctcctata tggcttatca agataccgtg ggggcatgag
      361 gcggagaatg taatggcggc acctgttagg gcgcgaaccc atgcaaaact gggctgcgcc
      421 gcacttttgg cctgcctcgc cgttccggcg cttgctcaga ctgttcctct gcccgtgccg
      481 cgtccgtcag ttctgcagac ccacgccggc accccttcat cttccacccc gaccgtcaag
      541 cctgcggtcc cgaaccaggc gctcggtgcg cgcgcgcaag cacctcaacc tgcgccatcg
      601 cagaatccga tctcaaatcc cttcgccgca ctgcttggca aggcaggcgg cggcggcaag
      661 cttactccgg agcaacgcgc gatcgtcgag cgcgtcaaca gttacctgtc aggggtgcag
      721 accctgacgg gaaattttgt gcaggtcggt cccgacggca gccgtacgca gggcgatttc
      781 tacatttcaa agcctgggcg cgtccggttt gaatatgatg atcccagtcc gattgaattg
      841 atcgccgacg gctcatcggt cgtcgttcga gaccgccgtc tggcgaccca ggacgtctac
      901 ccgctgtcgc agacaccgct gcgtttcctt cttgccgagc gcgtcgacct gaccaaggac
      961 acaaatctgg tgtccgtcta tgccgacgac gtatttgtca ccgtcgttct tgaggaaaaa
     1021 aatgggatcg tcggcacgag ccgtctgatg atcatgttca gcgcgaagga catgcagctc
     1081 aagcaatgga ccgtcacaga tccgcaaggc tacgacacca cggtcgctgt ttataacctc
     1141 gataccagca agcggcccga tccagcgtta ttcaagatcg actacactcg ataccacaat
     1201 tgacggcacc cgctgctgta cagctcatca gagtaataaa aataagaggt ggatggctgc
     1261 gacagacgca ggcatgccgt tggaaaattc gggcatgacg tcatagattg atgatcatgt
     1321 ctctcaaagt taccacttgg aacatcaatt cagtacggct gcgcatcaat ctggtggcga
     1381 gttttttgaa gagcacccag cctgacgtcc tgtgcctgca agagacgaaa tgtccggacg
     1441 acgtctttcc tctcaagcgg ttccggcggc ttgggtacga gcatgcggcg ctcagcgggc
     1501 agaagggtta tcacggtgtc gcagttctct cgcgttttcc gtttgaacag gtcgaaacgc
     1561 aaagtttctg cggcaaggcc gactgccggc atatttcggt ggtgctgggc gagcgcgcgg
     1621 agctgaaagg ccctgtcact ttgcataatt tttacgttcc ggccggcggc gacgagcccg
     1681 acccggccgt caatccgaag ttcaaacata agctcgattt cctcgatgaa atgcgcggcc
     1741 acgcaggact gcgcccggag aaatcgcagc gcgccatcct tgttggcgat ctcaatgttg
     1801 ctcccctcga gcacgacgtg tggagccaca aacagatgat caaggtcgtc tcgcacacgc
     1861 ccattgaatg cgaaaaactt gttgcagcgc gaacatcggg cggctggatc gacagcatgc
     1921 gcgtgctgac gcccgagcct gaaaagctct acacttggtg gagctatcgc gcgatggaca
     1981 attggcgcgc cgccgatcgc ggccgacgat tggatcacat ctggacgtca gacgcgctcg
     2041 ccgaccgcgt ctcgacgatc aagatcggca agagctatcg cggagcgaaa agaccgtcgg
     2101 accacgtgcc ggtcacggcc acgatcgagc tctgaattct tccaccgaat tgggttaggt
     2161 cttgtcacgc gcctcgagcg cgcgtttcac gccggcgagc tctctcgacc aggactgcag
     2221 ccgctcggtc atgctttcgc gcatcttctc cgctgcgccc ggataaccct ccagcatctt
     2281 gcggaagaga ttgcgtgaga tacggacgac aaccgtcggt tctttcgcga tcgctgttgc
     2341 ctgacaaact gtgttggtca aaagcgccaa ttcgccgatc aaggtcccgc ggcccacggt
     2401 aatttctttg ccttcagaga gcgtgcccgg ctccagcaga agtgagcctt cctgaatgac
     2461 gtaaccgcca tccgcaagct caccggcgta gaacagcaca gcgccgctat gcagatgccg
     2521 ggtctccgcg ccaatcgcaa gaatgcgcaa tgcctgcttg ccaagcgcag ccaacggcgg
     2581 cacctgctcg aagaacgcaa cgtcctcttc gagactcata ggactctcaa tcgccgggct
     2641 tggatcacgg caccaattta tatccgccag cctctgtcac aaggatggct ggcgcggcgg
     2701 cgtctttctc gaccttctgg cggaggcggt agatgtgggt ctcaagcgtg tgggtggtga
     2761 cgccggaatt atagccccag acttcctgca gcag
//
LOCUS       DASSRL010000565         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1747255, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000565 DASSRL010000000
VERSION     DASSRL010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747255"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..379
                     /locus_tag="VFL49_11455"
     CDS             53..379
                     /locus_tag="VFL49_11455"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7912641.1"
                     /translation="MQQHAIVIADQNGVIQLWSDGAATLIGYSREDAVGQKLDLVVPP
                     EFREQHWHGFGNAMKGGALKSTGTFFDLPVRCRNGDTRTLRGQLHVLRSETNGPIGAM
                     AIFTSP"
     gene            complement(411..1142)
                     /locus_tag="VFL49_11460"
     CDS             complement(411..1142)
                     /locus_tag="VFL49_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7912642.1"
                     /translation="MSRILIGTSGWSYRSWQSRFFPKNLMVKHQLQFYATQFLTAELN
                     GVFYRTPTVDAVRGWRDGTPDDFVFAWKASKFITHWKRLTDKSRNSLVLLESRLRILG
                     PKAGPVLFQLPPQFTANRERLADFLRLLKPERRYAFEFRHASWYEQPVLDLLRAHDIS
                     LCLSDHRDAPAPWVATASFVYVRGHGPGGAYKGHYSDEALRALAKRIASWKHRSCDVF
                     VYFDNDQKSAAPLDARRLALLLGER"
     gene            1244..2356
                     /locus_tag="VFL49_11465"
     CDS             1244..2356
                     /locus_tag="VFL49_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET7912643.1"
                     /translation="MQTRAAVAWKAGDPLTIETIQIEGPKAGEVLVEIKATGICHTDA
                     YTLSGADPEGLFPAILGHEGAGIVREVGAGVTSLQVGDHVIPLYTPECRQCKTCLSRR
                     SNLCTAIRATQGKGVMPDGTSRFRCDGDPVFHYMGCSTFANFTVLPEIALAKVREDAP
                     FDKICYIGCGVTTGVGAVINTAKVWPGAHVAVFGLGGIGLNVIQGARLAGADKIIGID
                     TNPAKRAMAEKFGMTHFVNPDEVGRDKVVQAVVDLTGGGADFSFECIGNVQTMRQALE
                     CCHRGWGESIIIGVAGAGQEISTRPFQLVTGRVWRGTAFGGARGRTDVPKIVDWYMDG
                     KINIDDLITHTMPLDSINDAFDLMHEGKSIRSVVIY"
     gene            complement(2528..2623)
                     /locus_tag="VFL49_11470"
                     /pseudo
     CDS             complement(2528..2623)
                     /locus_tag="VFL49_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
     gene            complement(3088..3189)
                     /locus_tag="VFL49_11475"
                     /pseudo
     CDS             complement(3088..3189)
                     /locus_tag="VFL49_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
BASE COUNT          716 a         1119 c         1121 g          648 t
ORIGIN      
        1 cagctgagga aggcaaaaag gaaacggcat aagagccgtg accgggtgaa caatgcagca
       61 acacgcgatc gtcatcgccg accagaatgg cgtcattcag ctttggagcg acggcgcggc
      121 gacactgatc ggctattcgc gcgaggacgc ggtcggacaa aagctcgatc tcgttgtgcc
      181 gccggaattc cgcgagcagc actggcatgg cttcggcaat gcaatgaagg gcggcgcgct
      241 caaatcgacg ggcacatttt tcgatctgcc ggtgcggtgc cgcaacggcg acaccaggac
      301 gcttcgtggc caactccatg tcctccgcag cgagacaaat ggcccgatcg gcgccatggc
      361 gattttcaca tctccgtagc gctaaatcgc cgtcactcct gcggaagcta ctaacgctca
      421 cccagcaaca gagccagccg ccttgcgtcg agaggcgctg cgcttttctg gtcgttatcg
      481 aaataaacaa agacatcgca gctgcggtgc ttccagctcg caattctttt cgccaaagcg
      541 cgcaaagcct cgtcggaata atgccccttg taggcgccgc ccggtccgtg cccgcgcaca
      601 tatacgaatg acgcggtggc aacccagggc gccggcgcgt cacggtgatc ggaaagacac
      661 aacgaaatgt catgcgcccg caacaggtca aggaccggct gctcgtacca gctggcgtgc
      721 cggaattcga atgcatagcg gcgttccggc ttgagcagcc tcagaaaatc ggcaagacgt
      781 tcacgatttg cagtgaactg cggcggcaac tgaaaaagga caggtcccgc cttagggccg
      841 agaatgcgca ggcgggactc aagcagcacc aggctgttac gcgatttatc ggtcagccgc
      901 ttccagtgcg tgatgaattt cgaggccttc caggcaaaga cgaaatcgtc cggcgttccg
      961 tcacgccagc cgcgaacggc gtcgaccgtt ggcgtgcgat agaacacgcc gttgagttcg
     1021 gccgtcaaaa attgcgtggc gtaaaattgc agctgatgct tcaccatcaa atttttgggg
     1081 aagaaacggc tttgccagga gcgatagctc catccggacg tgccgatgag aattctggac
     1141 atcaccagcc cttcgcgtag gcttgctcgt ctgtccggga cacaacaacg tttcggaagc
     1201 cgtatcggtc cgaacgcagt tggacggaag tcaaggagag cctatgcaaa cgcgtgccgc
     1261 ggtggcctgg aaggcgggag accctctcac catcgagacg atccaaatcg aaggcccgaa
     1321 agccggcgaa gtcttggtcg agatcaaggc gaccggcatc tgccacaccg acgcctacac
     1381 gctgtcgggc gcggatccgg aaggcctgtt tccggcaatt ctcggccacg aaggcgcggg
     1441 gatcgttcgc gaggtgggcg cgggcgtcac cagcttgcaa gtcggcgatc acgtcatccc
     1501 gctctacacg ccggagtgcc ggcaatgcaa aacgtgcctg tcgcgccggt caaatctctg
     1561 cacggccatc cgcgcgacgc aggggaaagg cgtgatgccg gacggcacca gccgcttccg
     1621 ctgtgacggc gatcccgttt ttcactacat gggttgctcg acctttgcga atttcaccgt
     1681 gctgcccgag atcgcgctgg caaaagtccg cgaagacgcg cccttcgaca agatctgcta
     1741 catcggttgc ggcgtgacga caggcgtcgg cgccgtcatc aacactgcaa aagtgtggcc
     1801 gggggcacat gtcgccgtgt tcgggcttgg cggcatcggt ctcaacgtca tccagggcgc
     1861 acgcctggcc ggcgccgaca aaatcatcgg catcgatacc aatcccgcca agcgcgcgat
     1921 ggccgaaaaa ttcggcatga cgcattttgt caatccggat gaagtcggcc gggacaaggt
     1981 tgtgcaggcc gtcgtcgacc tcaccggcgg cggcgccgac ttctcgttcg aatgcatcgg
     2041 caacgtgcag accatgcgcc aggcgctgga atgctgtcat cgcggctggg gcgaaagcat
     2101 catcatcggc gtcgccggcg ccggccagga aatctcaacc cgtccgttcc agctggtcac
     2161 cggccgagtc tggcgcggca cggccttcgg cggcgcacgt ggacgtaccg acgtgcccaa
     2221 aatcgtcgac tggtacatgg acggcaaaat caatatcgac gacctgatca cccacaccat
     2281 gccgctcgac agtatcaacg acgccttcga cctcatgcac gaaggcaagt cgatcagatc
     2341 ggtggtgatt tactagagcc taccagccgc ggcttcggcg gaacgggagc atgtcgtccg
     2401 cgtcgtcgtc ttcgtcgtag acgcgccacc gcgatctgcg atccacgtca cgtgaatcat
     2461 cgcgccggtt ccaggagcga cgctgcgact cgtctctttg ctcgcgattg gcgcgctgct
     2521 cgggcgcctt gctgcaaacg cggcgcggac cgtcatacgg ctgatatgta caatccgaag
     2581 cacggaacga tttgtaggcg cttgcgcagg cctggacgtc gcagcgattg ttggacgccg
     2641 ccgcttcggg ctgcgccgca tatgcctgcg cgttttcggc gctgtctccc tgaccagtac
     2701 ttgcggcgtt ggctgaatcg acagagcgcg tcgagccgcc cgtggtttcc gctgcaggct
     2761 tctggcccgt tccggttttc gccgcggtgt cgttagccgg caccggctcc agttttttcg
     2821 gatagaccgg gctgagctcc gcgttcgggc cttcgacctt gggacgcgcg ggcgccgccg
     2881 acgtctgcac gcccggtttt ggctgcgggg cgggtgtggt gagccaatgg atttcaagca
     2941 gcaccgtgct gaccgaaacc ataaggacgg taaggtagac gagaaattgc atgcttggcc
     3001 cgcgtgtgga cggcgcccaa gcaaacgcag gtatccgcgc ggcggttcca gttgctccgc
     3061 acggaactgt cgaagttgct gcagtcacta cttcttcgtg caaagccgtc gtggcccatc
     3121 atttggctga taggtgcagt cggatgcgcg aaacgaacgg taagcggccg tgcaggcgtc
     3181 gacattgcaa cgcacttccg gctcgggcgc agccgccggc gtctgcgcga cggctccggc
     3241 ggctaccggg gctgtcgcgg ccggcgcggc caatgtctgc ggaactatcg gcgcgccaat
     3301 cgtcgcactc gggactgatg gcgaagcctg cacggcggga gcttgcaaag caggcgccgg
     3361 cacggcggcc aggcctgccg cggtatcgac catgggagac atcggcgccg gaagccgatc
     3421 gaggcccagc agcacgctgc caagagcaat caccagcacg gcaccgtaaa ccgccagccg
     3481 catgttttgt cctcggcgtc ataataccac ggggaccatt tcaggaccta ttcgatttgc
     3541 ccttgaccgc cggttgcgcg cattccacga atgcagccgc gaagccgcat ggctgcacac
     3601 ctat
//
LOCUS       DASSRL010000566         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1292134, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000566 DASSRL010000000
VERSION     DASSRL010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292134"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFL49_11480"
     CDS             complement(<1..672)
                     /locus_tag="VFL49_11480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7912644.1"
                     /translation="MTIVDIYCHIYPDRFFREMTEVAPKLENICKRLRGVTKLFDLDA
                     RFKEMDEFGDYREIISLPNPAIEDIAPPDVGLNLARIANDSMAELCRKHPDRFPAFAA
                     ALCLTDVEGSVAEAHRAVKDLGARGVLMYTNVAGEPLDQPKFEPIFATMAELDLPIWL
                     HPVGTAAMPDYPAEKKSRFEMWWCFHWPYETSVAMVRMVFWGLFDRYPKLKIITHHLG
                     GMIPYY"
     gene            complement(669..1685)
                     /locus_tag="VFL49_11485"
     CDS             complement(669..1685)
                     /locus_tag="VFL49_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912645.1"
                     /translation="MTVEHATRSSRIGVALIVIALVVLALAPYWGDRQTLRLLAEMFT
                     FVALASLWNLLAGYAGLVSVGQQAFVGLGGYVLFALAILLDVNPIVAVLAAGPVGALI
                     AVPVAVLIFRLRGAYFAIGTWVVAEVFRLLASQVSALGGGSGISLPASVVLAMAPTRQ
                     MREFEMYWLALLLTIAILAAIFALLRSRHGLALTAIRDNELAAQSTGIGVWAIKFAVY
                     VLTAFGTAMVGAFIFLQKLRISPDTAFSVNDWTAFVIFITVIGGIGRIEGPIIGTIIF
                     FALRQSFAGLGSLYLLVMGAVAIVVMLWAPKGLWGLIVEQLGWQVLPLQRHLVLGKDA
                     RGKS"
     gene            complement(1682..2548)
                     /locus_tag="VFL49_11490"
     CDS             complement(1682..2548)
                     /locus_tag="VFL49_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912646.1"
                     /translation="MQWLNVILQGVLIGGLYAMFAAGLSLIFGVMRLVNIAHGDLIVL
                     AAYVSLMATQAFGVDPLTSLVLVVPVMALIGYVLQRGLLNYTLGDDLLPPLLVTFGLS
                     IIIQNGLLELFTADSRKLQAGAIEVQTFQLTQNLWIGALPLIQFVVAVAVIAGLQLVF
                     YRTALGRALRATSDDQAVAQLMGLDTRHVFAVAMALSLAVIAIAGVFLAVRANFDPAI
                     GPARLIFAFEAVIIGGLGSMWGTLAGGIILGVSQAIGAQIDPGWQLLAGHIVFLAILA
                     VRPQGLFPKVSG"
     gene            complement(2553..>3207)
                     /locus_tag="VFL49_11495"
     CDS             complement(2553..>3207)
                     /locus_tag="VFL49_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912647.1"
                     /translation="FQALFGISLAVDAGQVAAIIGANGAGKSTLLKCIAGLMYPARGE
                     IFFEDVARTNMPAFEASRRGIALVPEGRRLFPSLTVEENLLIGGQSRRPGPWTLARVY
                     ELFPVLAERRNLPAPALSGGQQQMAAIGRALMGNPRLLLCDEISLGLAPIVVRDIYAR
                     LPQITAEGLSLIVVEQDIVQALRAASHVYCLQEGRVALQGPAQDLSREKISSAYFGF"
BASE COUNT          748 a          979 c          945 g          535 t
ORIGIN      
        1 atagtaggga atcatcccgc caaggtgatg cgtgatgatt ttcagcttcg ggtagcggtc
       61 gaacagtccc caaaatacca ttcgcaccat ggcgaccgac gtctcatagg gccaatggaa
      121 gcaccaccac atctcaaagc gtgatttttt ctcggccgga tagtccggca tcgcggctgt
      181 gccaaccgga tgcagccaga tcggcaggtc gagttcggcc atcgtggcga aaatcggctc
      241 gaatttcggc tggtcgagcg gttcgccggc cacgttggtg tacatcaaga cgccccgtgc
      301 tccgaggtct ttgacggcgc ggtgggcctc ggcaacagaa ccttccacgt cggtcaggca
      361 caaagcggct gcaaaagcgg ggaagcggtc gggatgcttg cggcacagct cagccatcga
      421 gtcgttggca atacgggcga ggttgaggcc gacgtcgggc ggcgcaatat cctcgatcgc
      481 cgggttcggc agggaaatga tctcccggta gtcgccgaac tcgtccattt ccttgaagcg
      541 cgcatcaagg tcgaacaatt tggttactcc tcgcaatcgc ttgcagatat tctcaagctt
      601 tggtgcgacc tcggtcatct cccggaaaaa gcgatctgga taaatgtgac aatagatgtc
      661 gacaatggtc atgactttcc tctcgcgtct tttcccagca ccaagtgccg ctgcagtggc
      721 agaacctgcc aaccgagctg ctcgactatc aatccccaca ggcccttcgg cgcccacaac
      781 atcacgacga tggcaactgc acccatcacg agcaagtaga ggctgccgag ccccgcgaag
      841 gattgccgta gggcaaaaaa gatgatcgtg ccgataatcg gcccttcgat tctgccgatg
      901 cctccgatga ccgtgatgaa aatgacgaat gccgtccaat cattcacact gaacgctgtg
      961 tctggcgaaa tgcgcagttt ttgcaggaag ataaaggccc cgaccatcgc ggttccgaaa
     1021 gctgtcagca catagaccgc aaacttgata gcccatacgc cgattcccgt gctctgtgcg
     1081 gccaactcat tgtcgcggat tgcagtgagc gcgagcccgt gacgcgaacg cagcagcgca
     1141 aagattgctg ccaggatcgc gatcgtcagc agcagggcaa gccaatacat ttcgaactcg
     1201 cgcatctgcc tggtaggagc catggcgagg accaccgatg ccggaagact gatgcccgag
     1261 ccgccgccaa gggcggagac ttgcgaagcg agcagccgaa acacctcggc gacgacccag
     1321 gtcccgatcg cgaaataggc gccgcgcagc cggaagatca gcacggcgac aggaaccgca
     1381 atcaaagcgc cgaccggtcc ggccgccaga acggccacaa tcgggtttac atcaagcaaa
     1441 atggcaagcg cgaacaggac gtagccgcca aggccaacga aggcctgctg tccgaccgac
     1501 acaagtccgg cgtatcccgc aagcagattc cacaggcttg ccagcgcgac aaaagtgaac
     1561 atctcggcaa gcagtctcaa cgtctgccgg tcgccccaat acggcgccaa ggccagcacg
     1621 acaagcgcga tgacgataag cgcaacgccg atacggctcg agcgtgtcgc gtgttcgacg
     1681 gtcatcccga caccttggga aacaggcctt gcggccgcac agcaaggatc gcgagaaaca
     1741 cgatgtggcc ggcgagcagt tgccagccgg ggtcgatctg cgcgccgatc gcctgcgaga
     1801 caccgagaat gatgccgccg gcgagcgttc cccacatgct gccgagtccg ccgatgatca
     1861 ccgcctcgaa agcgaagatc agccgcgccg gtccaatggc cgggtcaaag ttggcgcgca
     1921 cggccagaaa cacgccggcg attgcaatca ccgccaacga cagggccatt gccaccgcga
     1981 agacgtgacg cgtgtcgaga cccatcaact gagcgacagc ctggtcatcg gatgtcgcgc
     2041 gcagcgcgcg gcccaacgcc gtgcggtaga agacgagttg cagacccgca atgactgcaa
     2101 cggcgacgac gaactgaatc agaggcaaag cgccgatcca gaggttctgc gtcagttgga
     2161 atgtctgcac ttcaatggcg cctgcctgca gcttgcggct gtcggcagtg aatagttcaa
     2221 ggagtccgtt ctggatgata atggacagac caaacgtcac cagcagcggt ggcagcagat
     2281 cgtcacccag cgtgtagttg agtaaaccgc gttgcagcac gtaaccgatc agtgccatga
     2341 ccggcacgac gagcaccaag gaggtgaggg gatccacgcc gaatgcttgc gtggccataa
     2401 gcgaaacgta ggccgcaagt acgataaggt cgccgtgcgc gatattgacc agccgcatta
     2461 ccccgaaaat cagcgagagg cctgccgcaa acattgcgta caagccgccg atcagcacgc
     2521 cttgcaggat gacattgagc cattgcatcc ggtcaaaacc cgaaataggc actcgaaatt
     2581 ttttctcgac tgaggtcttg tgcagggccc tgtaacgcaa cgcggccctc ctgcagacaa
     2641 tagacgtgac ttgcggcgcg cagcgcctgc acgatgtcct gctcgacaac gatcagggac
     2701 agcccctccg cagtgatctg cggcaggcgc gcatagatgt cgcgcacgac gatgggggcg
     2761 agaccaaggc tgatctcgtc gcacagcagc agccgcggat tgcccatcaa cgcgcggccg
     2821 atcgcggcca tttgctgttg gccacccgag agtgcgggcg caggaagatt tcggcgctcc
     2881 gccagaaccg ggaaaagttc gtacacgcgc gccagagtcc acggacccgg tcgtcttgat
     2941 tgcccgccaa tcagcaggtt ttcctcgacg gtcagcgatg ggaaaagcct gcgcccttca
     3001 ggaacaagcg caattccgcg tctcgacgcc tcgaacgccg gcatattcgt cctggcaaca
     3061 tcctcgaaga aaatttcgcc acgggcgggg tacatcaagc cggcgataca tttgagcaat
     3121 gtactcttgc cggctccgtt ggcgccgatg atcgctgcaa cctggccggc gtcaacggca
     3181 agcgagatgc cgaagagtgc ctgaaag
//
LOCUS       DASSRL010000567         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1274805, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000567 DASSRL010000000
VERSION     DASSRL010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274805"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFL49_11500"
     CDS             complement(<1..945)
                     /locus_tag="VFL49_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET7912648.1"
                     /translation="MQEQGMRNSAYGADKFGFKNLAAVHWNLTEPLLYEQAIAAGEAS
                     IVAGGALCAETGHHTGRSPKDKHTVVDALTENSVWWDGNRKMSKDNFDNLLADFLKHA
                     EGKKLFAQDLYGGADPKYRIRTRIFTELAWHSLFIRQLLIRPQRADLAGFVPELTILD
                     FPSFKPDATRHGGRDGSDTMVAIDFTRKIVLICGSSYAGEMKKSVFTTLNFYLPAQDV
                     VPMHCSANVSHDGESALFFGLSGTGKTTLSADPNRILIGDDETGWGPDGIFNFEGGCY
                     AKTIKLSQEAEPMIWDATNRFGAVLENVVFDAVTRVPNY"
     gene            complement(1173..1922)
                     /locus_tag="VFL49_11505"
     CDS             complement(1173..1922)
                     /locus_tag="VFL49_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HET7912649.1"
                     /translation="MTPDNTFNPRLAAKKLLREGRSGALATLMQGSGDPYCSLVNVAT
                     TVSGAPVLLLSKLALHTKNILTDARVSLMLDERKERDPLEGARVMLMGHCVKDDGAGT
                     PIAYLRRHPDAEMFANFADFSFYRMEIARAHLVAGFGRIVDLKPEDVLTDVAGAEGLV
                     DAEADAIAHMNTDHADACRLYATNLLGAADGDWRCVGIDPEGIELQNGRIALRLSFPQ
                     RITASGPLRGMLKSLAEKARAKQSNDPLASN"
     gene            2084..2782
                     /locus_tag="VFL49_11510"
     CDS             2084..2782
                     /locus_tag="VFL49_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7912650.1"
                     /translation="MPTIALVDDDRNILTSVSMALEAEGYRIMTYTDGASALDGFKTS
                     PPDLAILDIKMPRMDGMETLRRLRQKTDIPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAVLRRATPKDGAPPKEVDSAKVLERGQLRMDPERHTCTWKGEPVILT
                     VTEFLILQALATRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDDDFE
                     MIETLYGVGYRFKE"
BASE COUNT          623 a          886 c          890 g          606 t
ORIGIN      
        1 gtagttgggt acgcgcgtca ccgcgtcgaa caccacgttc tccagcacgg cgccgaagcg
       61 gttggtcgcg tcccagatca tcggctcggc ttcttgcgag agcttgatgg tcttggcgta
      121 gcagccgcct tcgaaattga agatcccgtc cggtccccaa ccggtctcgt cgtcgccgat
      181 caggatgcgg ttcggatcgg ccgagagcgt tgtcttgccg gtgccggaaa ggccgaagaa
      241 cagcgcgctc tcgccgtcgt gactcacgtt cgccgagcaa tgcatcggca cgacgtcctg
      301 cgccggcaga tagaaattga gcgtggtgaa gaccgatttc ttcatctcgc cggcgtagga
      361 cgagccgcag atcagcacga tcttgcgggt gaaatcgatc gcaaccatcg tgtcggagcc
      421 atcgcggccg ccatggcgag tcgcatcggg cttaaaagac ggaaaatcga gaatggtaag
      481 ctcgggcacg aatccagcga gatcggcgcg ttgcggccgg atcagcagct gacgaataaa
      541 cagtgagtgc catgcaagct cggtaaagat acgggtcctg atccggtatt tcggatcggc
      601 gccgccgtaa agatcctgcg cgaaaagctt cttgccttcc gcatgcttga gaaaatcggc
      661 cagaagattg tcgaagttgt ccttcgacat cttgcgattg ccatcccacc agaccgaatt
      721 ttcggtcagc gcatcgacga ctgtgtgctt gtccttcggg ctgcggccgg tgtggtgacc
      781 ggtctccgcg cagagggccc cacccgcgac aatcgacgcc tcgcccgcgg cgatggcttg
      841 ctcgtaaagc agcggctcgg tcaggttcca gtggactgcg gcgagatttt tgaaaccgaa
      901 tttgtcagcg ccataggcgc tgtttcgcat accctgctct tgcacgcgaa ttcctcccaa
      961 ctcagccgct tcgataattc cctcgcgggg tctttgggtc ccgccggcgg ctcattacca
     1021 gcttattata acccctcggt cagaggcgcg cgaccatagt caggggcagt gttaaagcca
     1081 agccaaaatg ccatgcaaag gcgtcgggcg gcgttgcact gcatcaataa gttaatgcgg
     1141 tcggcggtcg ctggggatta atgcttaatg tgtcagttcg acgctaacgg atcgtttgat
     1201 tgctttgcgc gcgccttttc agcgagcgat ttaagcatcc cccgcaacgg acctgacgcg
     1261 gttatgcgct gcggaaacga tagccgcagg gcaatacgcc cattttgcaa ttcaatcccc
     1321 tcggggtcga tgccgacgca tcgccagtct ccatccgccg caccaagcag gttggtcgca
     1381 tagagacggc aggcgtcggc gtgatcggta ttcatatgcg ctatcgcatc ggcttccgca
     1441 tcgacgaggc cctccgcgcc ggcgacatcg gtcaaaacgt cctctggctt gagatcaacg
     1501 atacggccga agccggcaac caagtgcgcg cgtgcgattt ccatccgata aaatgaaaaa
     1561 tcggcgaagt ttgcgaacat ctccgcgtcc gggtgacgtc tcaaataggc gatcggcgtg
     1621 ccggctccgt cgtctttcac gcagtgtccc atgagcatga cgcgcgcgcc ttcaagtgga
     1681 tctctctcct ttcgctcatc aagcatcagt gacacacgcg catcagtgag gatattcttg
     1741 gtgtgcaatg ccaatttcga cagcagcagc acgggtgcgc cgctaacggt ggttgcgacg
     1801 ttcacaagcg agcaataagg atcgccggag ccttgcatga gcgttgcaag cgctcccgaa
     1861 cgcccttcgc gcagcagctt cttggctgcc agccgaggat tgaatgtatt atcgggcgtc
     1921 atgccaaaac cgctggagca ttcacgattg tagcccgaac gcaggttttc ggctctcggg
     1981 cttgaaatcg ctgccgaatc acatagttgt tgcaaagagg ccacatttcg gaccccgttt
     2041 atagggttca agacgcgcgg gacgtcacgc gccagagggg ctcatgccga caatcgctct
     2101 cgtcgacgac gaccgcaata ttctgacttc cgtttcgatg gcactcgaag cggagggtta
     2161 ccgcatcatg acttataccg acggcgcttc ggcgctcgac ggctttaaga catcgccccc
     2221 ggatctcgcc attctcgaca tcaagatgcc gcgcatggac ggcatggaga cgctgcgccg
     2281 tctgcggcag aagacggaca ttccggtgat cttcctcacc tcgaaggacg aggagatcga
     2341 tgaattgttc ggactcaaga tgggtgcgga cgatttcatc cgcaaaccgt tttcgcagcg
     2401 cttgctggtg gaacgcgtga aagcagtgtt gcgccgggcc acgccgaagg atggagctcc
     2461 gcccaaggag gtcgattccg ccaaggtgct ggagcgcggg cagctgcgca tggatccgga
     2521 acggcacaca tgcacctgga aaggcgagcc ggtcatcctg accgttaccg agttccttat
     2581 cctgcaggcg ctggctacgc ggcccggcgt cgtcaaaagc cgtaatgccc tcatggatgc
     2641 agcctacgac gatcaggtct atgtggacga ccgcacgatt gacagccaca tcaagcgcct
     2701 gcgcaaaaag ttcaaagtcg tcgacgacga cttcgagatg atcgagacgc tttatggtgt
     2761 cggctaccgc ttcaaggaat gacaaatcct taatgtttca ggccgcttga gcggcctgac
     2821 ggagcgtccc ggcttgcggt aaaatccccc ggatcggatt ggatcacgat caacccggct
     2881 ggattaaacg acgtgcttga tcgaaccacg gaaaaagtcg tgcgcgacgc cgagccggaa
     2941 ggctccggcg cagcgaccgc gcgtccgcag ccgaagcaag cgtcggccgt gcgccgcatg
     3001 gtgcg
//
LOCUS       DASSRL010000568         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1292181, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000568 DASSRL010000000
VERSION     DASSRL010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292181"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFL49_11515"
     CDS             <1..344
                     /locus_tag="VFL49_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7912651.1"
                     /translation="GSTGLDDLHAALKKVGQSFNGFLAFTDGSDGVYWLENGEVRHIE
                     AFKVKAIDTLGAGDVFHGAFTFRFVESGDLRDAMRFAAAAAAIKCTRFGGLMGAATRA
                     EVNDFLAHNSA"
     gene            complement(505..1653)
                     /locus_tag="VFL49_11520"
     CDS             complement(505..1653)
                     /locus_tag="VFL49_11520"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HET7912652.1"
                     /translation="MTTASLAAPDQGAAVRIDRLFLGLALAATAILVALVLLDHQPAS
                     AALIIGGFGLGVAFLKAEFSYTASWRHFLTRGEAGGLLGGLIVIAVCAVVVVPVAALL
                     PKYGGAIAPLGPSLIIGAFIFGIGMQLANGCGSGTLYTVGGGSGRMLVTLLFFVIGSV
                     FGSLSLPSFLALGGIDPILASDYLGAWGGLIATLASIAVAALLIVAVARRRGANVMPS
                     RNYIIGGLAIGLLCIAVFLAGGHPWSVTFGYTLWGAKAATALGFDLSHSAFWQWPGPR
                     HALAESVLSDTSSLTDFGMIFGAMAAAAASKPFARASWPPAKSLLAAGVGGLLMGWGA
                     RLGFGCNIGAFVGGIASGSLHGWIWFLAALPGCLIGIRLRPLFGLSRE"
     gene            complement(1673..>2172)
                     /locus_tag="VFL49_11525"
     CDS             complement(1673..>2172)
                     /locus_tag="VFL49_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7912653.1"
                     /translation="GLPVETGMKAPSPKIFTATVDKSILADASDVEAIEGKGGATLID
                     ARPASFFLGKEKAPASKAYGHIPGALNIDSAEFYDAKANRLKPKAELVAVANAAPAGP
                     VVNYCNTGHWASTDWFVFHEMLGRKDAKLYAGSMVEWTSNDGRPIESSRTKWDDLKKA
                     LGLGS"
BASE COUNT          460 a          703 c          657 g          352 t
ORIGIN      
        1 ggggaagcac cggactggat gatctgcacg ccgctctcaa aaaggtcgga cagtccttca
       61 atggctttct cgctttcacc gatggctcgg atggcgtgta ctggcttgag aatggcgagg
      121 tccgccatat agaggccttt aaggtgaagg cgattgacac actcggtgcc ggtgacgtct
      181 tccacggcgc tttcacgttc cgcttcgtgg agtccggtga cctgcgcgac gcgatgcgat
      241 ttgcggcggc ggccgccgcc atcaaatgca cccgcttcgg cggcttgatg ggcgcggcaa
      301 cgcgcgccga ggtcaacgat ttcctcgcgc acaattccgc ctgaacgcta tttctttgac
      361 ggacagggag cgccgcaagg cgcgcacgcg cccgcaaaat cttccgggga tacagcccgt
      421 ccgctcgatg ggctggagag atgatcgctc agtatcgcgc caatgcagag cactacgacg
      481 atgatataga cgacccgcac cgcgctactc ccgcgacaaa ccgaagagtg gccgcagacg
      541 aattccgatc aggcagcccg gcagcgcggc gaggaaccag atccacccat gcagcgagcc
      601 ggaggcaatg ccgccgacaa atgcgccgat gttgcagcca aacccgaggc gggcgcccca
      661 ccccatcagc agtccgccaa ctccggcggc gagcaatgat ttggcgggcg gccagctggc
      721 gcgcgcaaac ggcttcgatg ctgcggccgc cgccatggcg ccaaaaatca ttccgaaatc
      781 cgtgagactc gacgtgtcag agaggaccga ttcggcaagc gcatgcctgg ggcccggcca
      841 ctgccaaaat gccgagtgcg aaaggtcgaa gccgagcgcg gtcgcagctt tcgcgcccca
      901 cagcgtgtag ccgaaagtca cgctccaggg atggccgccg gccagaaaga cggcgatgca
      961 aagcagtcct atggcaaggc cgccgatgat gtagttgcgc gacggcatga cgtttgcacc
     1021 acgtctgcgc gcaaccgcga cgatcaacaa cgccgccacg gcaatgctgg cgagggtcgc
     1081 aatcaggcct ccccacgcac cgaggtagtc cgacgccagg atcggatcga tgccgccgag
     1141 tgcaagaaat gacggcagcg agaggctgcc gaatacgctg ccgatcacaa agaacagcaa
     1201 cgtcaccaac atgcggcccg accctccgcc gaccgtgtag agcgtccccg aaccgcagcc
     1261 gtttgccagc tgcatgccga tgccgaaaat gaacgcgccg atgatcaggg acggtccaag
     1321 cggggcaatt gccccgccat atttcggcag caacgccgcc accggcacca ccacaaccgc
     1381 acagacggcg atcacgatca gtccgcccag aagccctcct gcctcgccac gggtaagaaa
     1441 atgacgccat gacgcggtgt agctgaattc ggccttgagg aacgccaccc cgagaccaaa
     1501 gccgccaatg atcaaagctg ctgaggccgg ttgatggtca agcagcacca gtgcgaccag
     1561 gatcgccgtg gccgccagtg cgagccccag aaaaagccgg tcgatgcgaa cggccgcgcc
     1621 ctgatcgggc gcggccagac ttgcagtcgt cattggaact ccgaggcgca gatcaggatc
     1681 cgaggccgag ggccttcttc agatcgtccc atttcgtgcg cgaagattca atcggccggc
     1741 catcattgct ggtccactcg accatcgagc ccgcatacag cttcgcatcc ttgcggccga
     1801 gcatttcatg gaacacgaac cagtcggtcg atgcccagtg gccggtgttg caataattca
     1861 cgacaggacc ggccggagcc gcgtttgcga cggcaacgag ctcggccttc ggcttcaggc
     1921 gattggcttt cgcatcgtag aactcggcgc tgtcgatatt caacgcgcca ggaatgtggc
     1981 cataggcctt ggacgccggc gctttttctt tgccgaggaa gaacgatgcg ggacgagcat
     2041 cgatgagagt tgctccgccc ttcccttcga tcgcttcgac gtcactcgca tcggccaaga
     2101 tgctcttgtc tacggtcgcc gtgaaaatct ttggtgacgg cgccttcatt cccgtctcga
     2161 ccggcagacc gg
//
LOCUS       DASSRL010000569         4739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_767320, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000569 DASSRL010000000
VERSION     DASSRL010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767320"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFL49_11530"
     CDS             <1..606
                     /locus_tag="VFL49_11530"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET7912654.1"
                     /translation="STNAFTALAKRAEQDGVLAEKVGMRTDIPPRLFRQLLMQASDVV
                     QKRLLSKAKPETQAEIRRILAQVTDEVAAKAAPRNYTAALATVRALHKERKLGESDIA
                     EFANAGKYEETIAALATTCAVPVEVVDRLMNGDRADPVLILARAAGFGWPTVRAILKA
                     RPGPKPSPQSLDAARENFERLTATTAQRVVRFWQVRQGTGE"
     gene            complement(607..1206)
                     /locus_tag="VFL49_11535"
     CDS             complement(607..1206)
                     /locus_tag="VFL49_11535"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HET7912655.1"
                     /translation="MPKVALDLSKPAPPDLRMLFANAVSGVRLEIGFGGAEHLIAEAK
                     AHPQIGFIGTDAFVNSIAKALMAISDHALVNVCLYFGDASELIDWLPDAALLRIDLLY
                     PDPWPKRRHWKRRFIQDESLIRLARILTHGGELRFATDVGDYAAYALARVLRSKDFGW
                     TAECANDWRSPWAGFSGTRYEAKARREGRKPAYFIFERR"
     gene            complement(1464..2738)
                     /gene="metK"
                     /locus_tag="VFL49_11540"
     CDS             complement(1464..2738)
                     /gene="metK"
                     /locus_tag="VFL49_11540"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7912656.1"
                     /translation="MAHTNFLFTSESVSEGHPDKVCDQISDAVLDAFLENDVKLGIAD
                     DSLVNTRLGCETLATTNKIVIAGEGRGQAPLFRKHGDKAVVDRELITKIARDVVRDIG
                     YDQDGFSYYGADVEVLLHGQSPDIAMGVNAKKKKGGEEEGAGDQGMMFGFACTESEVY
                     EKNSFMPAPIYFAHKILKVLSEKRRSGQLYDLQPDSKSQVTVRYVDGKPIGCTKVVVS
                     TQHNAQSRNGKKYTPGLIKEMISDAVESALPKGWMPKRPSDFLVNPTGNFVIGGPDGD
                     CGLTGRKIIVDTYGGYAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGVAERCTI
                     QVAYAIGVADPMSVLVDTHGTGKVEEKKLEKVLPELFRLTPTNIRRTLKLNRPIYKRT
                     AAYGHFGRAPDKDGGFSWERTDLASAIRGAVR"
     gene            complement(2876..3277)
                     /locus_tag="VFL49_11545"
     CDS             complement(2876..3277)
                     /locus_tag="VFL49_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7912657.1"
                     /translation="MAKKAPNPIDRHVGSRVRMRRMMLSMSQEKLGDALGLTFQQVQK
                     YEKGTNRIGASRLQQIAHILQVPVSFFFEGAPHSPGHTGGMSEAPSPAYVADFLATSD
                     GLALTKAFMRIKNSKLRRRIVDLVEQIAGIN"
     gene            complement(3495..>4739)
                     /gene="lnt"
                     /locus_tag="VFL49_11550"
     CDS             complement(3495..>4739)
                     /gene="lnt"
                     /locus_tag="VFL49_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET7912658.1"
                     /translation="TGLSLAVARLIWVRAPERILALAAAMTAAEWLRGHLLSGFPWNT
                     FGYALTEPLVLAQSVSLTGIWTLTFLCVAICASPAVIADDKADTRHPYRPLVFGLVVL
                     AGLAGYGAVRLWLHPTEYVDGVRLRIMQPNLQQDDKFNYAAKAAVMSRYLRLSDRATT
                     PRSNGVRDVTHLIWPEAAFPFFLMREPDALAQIAGLLKPTTELITGAVRAASDANVRD
                     QRAYNSIYVIDPDGSIRGIYDKVHLVPFGEYLPFQRVLERLGLMQLTKVIGGFLSGDR
                     RRPMDVAGAPKMLPLICYEAIFPGAAVPPGERPGWMVNVTNDGWFGMSSGPYQHFQQA
                     RVLAIAEGLPLVRAANTGISAVVDPVGRIVGSLPLGVEGVLDARLPAAIEPTLYLKYG
                     NYLLGAALILSLLIVTRRRFRA"
BASE COUNT          955 a         1406 c         1433 g          945 t
ORIGIN      
        1 tcgaccaacg cgttcacagc gcttgcgaag cgcgcggagc aagacggcgt tcttgcggaa
       61 aaggtcggca tgcgcaccga catccccccg cgtcttttcc gtcagctgtt gatgcaggca
      121 agcgacgtcg tgcagaagcg cctattgtcc aaagcaaaac ctgaaacgca ggcggaaatc
      181 cggcgcattc tcgcgcaggt caccgatgaa gtcgccgcaa aagccgcacc gcgcaattat
      241 acggcggccc ttgccacggt tcgcgcattg cacaaggagc gcaagctcgg cgagagtgat
      301 attgccgaat tcgccaatgc cgggaaatac gaagaaacca ttgcagccct ggccaccaca
      361 tgtgctgtac cggtcgaggt cgtggatcgg ctcatgaacg gcgaccgcgc cgacccggtc
      421 ttaatcctgg cgcgtgcagc cgggttcggc tggccgacgg tgcgggccat tctcaaggct
      481 cgtcccggcc cgaaaccctc gcctcagtca ctcgatgcag cgcgcgaaaa tttcgagcgg
      541 ctgacggcaa cgactgcaca acgtgtcgtg cggttctggc aggtgcggca gggcaccggt
      601 gaatagtcag cgccgctcga aaatgaaata ggccggcttg cggccttcgc gtctggcttt
      661 agcctcgtat cgcgtcccgg aaaatcccgc ccacggactg cgccagtcgt ttgcgcactc
      721 ggcagtccag ccaaaatctt tcgagcgcaa gacccgcgcc agcgcatagg cggcgtagtc
      781 gccaacgtcg gtggcaaatc gcaactcgcc gccgtgcgtc aggatgcgag ccagtcggat
      841 caggctttca tcctggatga atcgccgctt ccagtgacgt cgcttcggcc atgggtcagg
      901 gtagagtagg tcgatgcgca acagcgctgc atccggcagc cagtcgatga gttcgctggc
      961 atcaccgaaa tataggcaga cattgaccag cgcgtggtca ctgattgcca tcaatgcctt
     1021 ggcgatcgaa ttgacgaagg catccgtgcc gataaatccg atctgcgggt gggcctttgc
     1081 ctcggcgatc aggtgctcgg cgccgccaaa tccgatttcg aggcgcacgc cagaaaccgc
     1141 gtttgcaaac agcatgcgga gatcgggagg ggccggtttg ctgagatcaa gggcaacttt
     1201 cggcaatagc gtgtcgaaaa gcgctgactg ccgcggcttg agcgcatgtc ccttccgacg
     1261 gccgaaaaac gcccgctgtg ttgatggtct gccgtgatcc atcactcgtc atgcctggcc
     1321 atagccgtcc gaaggacagc gtgctttcgc acccctatgt accggcatcc acgtttttct
     1381 tccctgtcat agtgaagcaa gacgtggatg gctgggacaa gcccgccatg acgaatcatt
     1441 cagcgatgtg tgcgtcgagc cgcctaccta accgcacctc ggattgcgga ggcgaggtcg
     1501 gtcctctccc aggagaagcc gccgtccttg tcaggcgcgc ggccgaaatg tccgtaggcc
     1561 gcagtgcgct tgtagatcgg ccggttgagc ttgagcgtgc ggcggatgtt cgtcggcgtg
     1621 agccggaaca actccggcag gactttctcg agcttcttct cctcgacctt gcccgtgccg
     1681 tgggtatcga ccagcactga catcggatca gcgacgccga ttgcataagc gacctgtatc
     1741 gtgcagcgct cggcaacgcc ggcagcgaca acatttttgg caagatagcg ggcggcgtag
     1801 gcggccgagc gatcgacctt ggtcgggtct ttccccgaga aggcaccgcc gccgtggggt
     1861 gcgtaaccgc cgtaagtgtc gacgatgatc ttgcggccgg tgagaccgca gtcgccgtcc
     1921 ggcccgccaa tcacgaagtt acctgtcggg ttgaccagga agtcggacgg cctcttaggc
     1981 atccaacctt tcggcagcgc cgactcgacg gcgtcggaga tcatctcctt gatcaggccg
     2041 ggcgtatatt tcttgccgtt gcggctctgc gcgttgtgct gcgttgacac cacgaccttg
     2101 gtgcagccga tcggtttgcc gtcgacatag cggacggtga cctggctctt cgaatcgggc
     2161 tgcaagtcgt acagctggcc ggaccggcgc ttttccgaaa gaaccttgag aatcttgtgc
     2221 gcgaaataaa taggcgcagg cataaacgag ttcttctcgt agacctcgct ctcggtgcag
     2281 gcaaagccga acatcatgcc ctggtcgccg gcgccttctt cctctccgcc ttttttcttc
     2341 ttcgcgttca cgcccatcgc gatatcgggg gactgtccgt gcagcagcac ctcgacgtcc
     2401 gcgccgtagt acgaaaagcc gtcctggtcg tagccgatat ctctgacaac atcgcgcgcg
     2461 atcttggtga tgagctcgcg atcgaccacc gccttgtcgc catgcttgcg gaacagcggc
     2521 gcttgaccac gcccttcgcc ggcgatgacg atcttgtttg ttgttgcgag tgtttcgcag
     2581 ccaagccgcg tgttcaccag gctgtcgtca gcaatgccca gcttgacgtc gttttccaga
     2641 aatgcgtcga gcaccgcatc agaaatctga tcacagactt tgtccggatg accttctgaa
     2701 acagactcgg aggtaaaaag gaaattcgtg tgagccaccg ctggaactcc tcccgtggcg
     2761 cggccgcgct gccgcgtcat gctatgtcgg ggtggttctt gcagcggcgg cgatgcaggt
     2821 caagatgcgg gcagcgttgg gcagatgcca caaaacgatg ggatacgacc ctcgttcaat
     2881 tgataccggc gatttgctcg accaggtcga cgatacgacg gcgcagtttg ctgttcttta
     2941 tgcgcataaa ggccttggtc aacgccagac catctgaggt cgcaaggaag tcggcaacat
     3001 aggccggcga cggcgcttcg ctcatgccgc cggtatggcc cggcgaatgc ggcgcgcctt
     3061 cgaagaaaaa tgaaaccggt acctggagaa tgtgcgcaat ctgctgcaag cggctggcgc
     3121 cgatgcggtt cgttcctttt tcgtatttct gaacttgctg gaacgtgagc ccgagagcat
     3181 cacccaactt ttcttggctc atgctgagca tcattcggcg catgcggacg cgtgaaccga
     3241 catgtctgtc gattgggttc ggcgccttct tagccataga tttctcctat gcccccattc
     3301 cgagcatagt tgtcacaagt gaaggcgcta acgcaatgcg cgagtccctg taatcgggaa
     3361 atggcgcgta aatttagtca tagatgtcag tggaatttgt atcactacat tccgatggta
     3421 ttttgtttag tggactattc tgctgcccga ttttcctaca gcaacttgca taatgctgtt
     3481 ggaaattgcg caatttaagc gcgaaatcgg cgacgggtta caatcagcag gctaagtatc
     3541 agagccgcgc ccaggagata gttgccatat ttcagataga gcgtcggttc gatggctgcc
     3601 ggcagccggg cgtccagcac gccttcgacc cccaagggca atgagccgac aatccgtccg
     3661 accgggtcga ccaccgccga aatgccggta ttggccgcgc gcaccagagg cagcccttcg
     3721 gcgatggcaa gcacacgcgc ctgctggaaa tgctgataag gccctgaact catgccgaac
     3781 cagccgtcgt ttgtgacatt gaccatccag ccaggccgct cgcccggtgg cactgcggca
     3841 ccgggaaaga tcgcctcgta gcagatcaaa ggcagcatct ttggcgcgcc ggcaacgtcc
     3901 atcgggcgcc ggcggtcgcc tgaaaggaat ccgccaatca ccttcgtcaa ttgcatcagg
     3961 ccgaggcgtt cgagcacacg ctgaaacggc aggtattctc caaagggaac gagatgcacc
     4021 ttgtcgtaga ttccgcggat cgaaccgtcg ggatcaatga catagatcga attatatgcg
     4081 cgctgatcgc gaacattggc gtccgacgcc gcccgcacgg cgccggtgat caattcggtc
     4141 gttggtttga gcaggccggc gatctgtgcg agagcgtcag gctcgcgcat cagaaaaaac
     4201 ggaaaagccg cctccggcca tatgagatgc gtcacgtcac gaacgccgtt cgatcgcggg
     4261 gttgtggcgc gatccgatag acgcaggtag cggctcatca cggctgcttt ggccgcgtaa
     4321 ttaaatttgt catcctgctg caggttgggt tgcatgatgc gcaggcggac gccgtccacg
     4381 tattccgtcg ggtgcaacca gagccgtacg gcgccgtaac cggcgagccc tgcaaggaca
     4441 acgagtccaa aaacgagcgg acgatatggg tgtcgcgtgt cggctttgtc gtcggcgatg
     4501 accgcgggac ttgcgcagat ggcgacgcag aggaaagtga gggtccagat tccggtcaat
     4561 gacacgcttt gcgccaggac gagtggttca gtcagcgcat agccgaacgt attccagggg
     4621 aagccactga gcagatgccc gcgcagccat tcggcggccg tcatcgcggc ggcaagcgcc
     4681 aggatgcgct caggcgcccg cacccagatc agccgggcaa cggcaaggct gagaccggt
//
LOCUS       DASSRL010000570         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_417756, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000570 DASSRL010000000
VERSION     DASSRL010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417756"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..978
                     /locus_tag="VFL49_11555"
     CDS             139..978
                     /locus_tag="VFL49_11555"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912659.1"
                     /translation="MAPAPSVNDAILVADDIVVRFETPEGAITAVDNVSLKVRPGEFL
                     SIIGPSGCGKSTLFNVIGGLLGQHQGVVTVAGETISGPHKSIGMVFQEESTFPWRTVT
                     DNVGFPLELVGMPKAQRIERARHFIKMVGLDGFENRYPGELSGGMRQRVSLARTLASE
                     PKILLMDEPFAALDEQTRLLLGDKVLQIQQQLKQTTLLITHNITEAVQMSDRILVMTY
                     RPGKVKRIVDISLPRPRTSEIVGSEAFGRYVAEIWNDLREEASRGMRDEEKHALEHQG
                     TAS"
     gene            975..1754
                     /locus_tag="VFL49_11560"
     CDS             975..1754
                     /locus_tag="VFL49_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7912660.1"
                     /translation="MTAAAVRWPRSRSLPPALVGIATLVVALLIAELLIQLNFISRFI
                     VPPPSEIIASFGRVIVEEHVIRRFFLTLGECLTAGVMLTVFGIAGGVLMHRIKLLQQA
                     CETWVAAMAAAPLVLAYPLFMVIFGRNAWTIIMMGFFAALPPVILKTIEGMSNTRKVL
                     VNVGRSFNLTPAQQFWKIMFPAALPTIFVGVRLGLIFALINIVGVEFLINYGGLGQLI
                     NDLAERYDLPGTYAAICFVILVSVLFFMALEKAERWLRPID"
     gene            1733..2527
                     /locus_tag="VFL49_11565"
     CDS             1733..2527
                     /locus_tag="VFL49_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912661.1"
                     /translation="MAATDRLTRTATTEPLVSPVTLLRIAIILAVLAIWEFLAHSGLL
                     YRDVVPSLFAIGKALYELLSRGDYYYNLGVTAGEIGIALLIGGISGLAVGILLGANRF
                     LSRAFEAYLYYLGPTPKIIFFPIMIMWFGVGPGSKIALGTLSCFFPVALSVAAGTRQI
                     DKVLIRVGKSFRASTWQMIMKIYLPAMRHPIINGVRLGLGVALIGTLLAETKLSNKGI
                     GFLIINAYSTFDMPRMYAMLIVLFVLAIGANALVGRLGGLESIKRT"
     gene            2552..3505
                     /locus_tag="VFL49_11570"
     CDS             2552..3505
                     /locus_tag="VFL49_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912662.1"
                     /translation="MKHVVGLAAAALAMATLTQPAVADDLVKMTIGQRGNWDTAITHL
                     GDKAGIFKKHGLQLEMIYTSGSGETLQPVISGSVDLGLAVGTLGAMAAYAKGAPVRII
                     GAEATGAADYWYAKNPAIKTLKDTNGHTIAFSTNGSSTNSVVRAFIDEFKLTARPQAT
                     GNPSATLTAVMTDQVDVGWAAPPFGLKEMDEGKIHLVARATDAALVRGQTIRVLVANA
                     DALVRRKEVIERFMNAYRESIDYIYSSNPQVLKDYAEFARISEPLAKRVRDEFFPKSL
                     VNPDQIHGLDTLIPEAVNLKFISAPLTKEQLTELIQISPRK"
     gene            3528..5024
                     /locus_tag="VFL49_11575"
     CDS             3528..5024
                     /locus_tag="VFL49_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7912663.1"
                     /translation="MTTAVSPQAASAQAVVLPKNRGAYYGGAWHDPKAGRFVDTISPG
                     TGQSLGKVADSGAEDIDAAVAAAKIAFAEWRRVLPLERAKILRRIAEILRQNANELAM
                     IDAADCGNPVKEMVSDAMIAAAQMEFFAGFVTEMKGASIPMGPDVVNFSVREPLGVVG
                     RIIPFNHPFMFCAGKSAAPLAAGNTVVVKPPEQAPLSSLRLAELIGGLLPTGAFNVVP
                     GGREAGQRLASHPDVAMIALIGSVPTGRAVMKAASDTVKRTMLELGGKNALIAYGDAD
                     PDEVAGAVIGGMNFTWCGQSCGSTSRAFIHETIYDAVVARVKEKIKYYRPGIPTDPAT
                     TMGAIVSRQQFDRVMSFIDSAKSEGAQLITGGGPPADPALKGGFYIEPTVFAVTDKNR
                     IAREEIFGPVLGVFKWSDESKMLATVNAVEYGLTASIWTNDLSTAHRTALAVQAGYVW
                     INEVSKHFLGAPFGGFKQSGLGREECFEEMLAFTQEKNIHVKLKAPRA"
     gene            complement(5093..>5779)
                     /locus_tag="VFL49_11580"
     CDS             complement(5093..>5779)
                     /locus_tag="VFL49_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7912664.1"
                     /translation="VGQILDALKQLGVDDNTLLVFASDNGPSGEAFREYGNAGTPDMG
                     SAGPFRGELGEVTEGSIRTFCFIRWPGHIKPDTTSYAMFSIMDFFPTFASITGAKMPS
                     DRPIDGVDQTDVLLGKSEAGKRDSLLSFIGGDLVAARWKQWRVYFTDVYPTGIGPQRQ
                     PGTGSASAPMAGYPKLFNIEMDPHEDLNVFGMFPFATEPAIKVVEEYLKSVRQYPNPP
                     APNITQFQSR"
BASE COUNT         1123 a         1784 c         1759 g         1113 t
ORIGIN      
        1 cccgacctgg cccccacact cgcgaaggcc attgcctcag tcgaaggcgg cgcggtggcg
       61 gtcgtcgatg tgcgggtgga gcccggttat acggccgcga cgaccgcggc gatgacccgc
      121 aaaagcgagt aagaccttat ggcgcccgcc ccatccgtca acgacgccat cctggtcgcc
      181 gatgacatcg ttgtgcgatt cgagaccccg gagggcgcaa tcacggctgt cgacaatgta
      241 tcgctcaagg tccggccggg tgaattcctt tcgatcatcg gcccgtccgg ttgcggcaag
      301 tccacacttt tcaacgtgat cggtggtttg ctcggccagc accagggcgt cgtcaccgtc
      361 gccggtgaaa cgatttcagg gccgcataag tcgatcggca tggtgttcca ggaagaatcg
      421 acgttcccct ggcggaccgt caccgacaac gtcggctttc cgcttgagct tgtcggcatg
      481 ccgaaagcgc aacgcatcga gcgggcccgc cacttcatca agatggtcgg gctcgacggc
      541 tttgaaaacc gttatcccgg cgaattgtcc ggcggcatgc ggcagcgtgt gtcgctcgcc
      601 cgcacgctcg cttccgagcc gaaaatattg ttgatggacg agcccttcgc cgcgctcgac
      661 gagcagacgc ggctcctctt gggcgacaaa gtacttcaga tccagcagca gctgaaacag
      721 acgacgctgc tgatcacgca caatattacc gaagcagtcc agatgtccga ccgcatcctc
      781 gtaatgactt accgtcccgg caaagtgaag cggatcgtcg acatcagcct gccgcgcccg
      841 cgcacgtccg aaatcgtcgg cagcgaagcc tttgggcgct atgtcgccga aatctggaac
      901 gacctgcgcg aagaggcgag ccgcggcatg cgcgacgagg aaaagcacgc gctcgaacac
      961 cagggcacag cgtcatgacg gccgcggcgg ttcgatggcc gcgatcacgc agtctgccgc
     1021 cggcgcttgt cggtattgcc accctcgttg tcgcgcttct gattgccgag cttctgatac
     1081 agctcaattt catcagccgt ttcatcgtgc cgccgccatc cgagatcatc gccagtttcg
     1141 ggcgcgtaat cgtcgaagag cacgtgatac gccggttttt cctgacactt ggcgaatgcc
     1201 tgacggccgg tgtcatgctc acggtgttcg gcatcgccgg cggcgtgctg atgcaccgca
     1261 tcaagctcct gcagcaggcc tgtgaaacct gggttgcggc catggcggcg gcgccgctgg
     1321 tgctggccta tcccttgttc atggtgatct tcggccgcaa tgcctggacc attatcatga
     1381 tgggcttttt cgcggcgctg ccgccggtca tcctgaagac gatcgaaggc atgtcgaaca
     1441 cgcgcaaagt gctggtcaat gtcggccgca gcttcaacct gacgccggcg cagcagttct
     1501 ggaagatcat gtttccggcg gcgttgccga caattttcgt cggcgtgcgg ctgggactga
     1561 tattcgccct gatcaacatc gtcggcgtgg agttcttgat caactacggc gggctcgggc
     1621 agctgatcaa tgatctcgcc gaacgctatg atcttcccgg cacctatgcg gcgatctgct
     1681 tcgtaatcct ggtgagcgtt ctgttcttca tggctctgga aaaggccgag cgatggctgc
     1741 gaccgatcga ttgacacgaa ctgcgacaac ggaaccgctg gtcagcccgg ttacgctcct
     1801 gcggattgcg atcatcctcg ccgtattggc gatttgggag tttctcgccc attccggctt
     1861 gctttatcgc gacgtggtgc cttcgctgtt tgccatcggc aaagcgctct acgagctgct
     1921 gtcgcgtggc gactactatt acaacctcgg tgtcaccgcg ggtgagatcg gaatagcgct
     1981 cttgatcggc ggaatttccg ggcttgcagt ggggatattg cttggcgcca accggttcct
     2041 gtcgcgagca ttcgaagcct acctttacta cctcggcccc acgccaaaga tcatcttctt
     2101 cccgataatg atcatgtggt tcggtgtcgg ccccggctcc aagatcgcgc tcggcaccct
     2161 ctcctgcttc ttccccgtag cgctttcggt tgccgccggc acgcggcaga tcgacaaggt
     2221 attgatccgc gtgggcaaaa gcttccgcgc gtcgacctgg cagatgatta tgaaaatcta
     2281 cctgccggcg atgcggcatc cgatcatcaa tggcgtcagg ctcggcctcg gtgtggcgct
     2341 gatcgggacg ctgttggccg aaaccaaatt gtcaaacaag ggcatcggat ttctgatcat
     2401 caatgcctac agcaccttcg atatgccgcg catgtatgcc atgctgatcg tcctgttcgt
     2461 gctggcgatc ggcgcgaatg ccctggtggg ccgtctgggc ggcctcgaaa gcataaaaag
     2521 aacctgagtt ctggtaaaac gggagcgacg catgaaacat gtggttggac ttgccgccgc
     2581 agcgctcgcg atggcgacgc tcacgcaacc ggcagtggcc gacgatctgg tgaaaatgac
     2641 catcggccag cgcggcaact gggacactgc gatcactcat ctcggcgaca aggccggcat
     2701 tttcaagaaa catggactgc agctcgagat gatctacacc tcgggcagcg gcgaaaccct
     2761 gcagcccgtg atctccggca gcgttgatct cggcctcgcc gtcggcacgc tgggcgccat
     2821 ggccgcctat gcgaaaggcg cgccggtgcg catcatcggc gcggaagcaa cgggagctgc
     2881 ggactattgg tacgccaaga atccggcgat caagacgctg aaagacacca acggacacac
     2941 gatcgcgttt tcgaccaacg gctcctcgac caacagcgtg gtgcgggcgt tcatcgacga
     3001 gttcaagctg accgccaggc cgcaggccac cggcaatccg agcgcaacgc tcaccgccgt
     3061 gatgaccgac caggtcgacg tcggctgggc agctccgccg ttcgggctga aggaaatgga
     3121 cgaaggcaag atccatctgg tggcgcgcgc gacagatgcc gcattggtgc gtggccagac
     3181 gatccgcgtg ctggttgcca atgcggacgc gctggtgagg cgtaaagagg tcatcgagcg
     3241 cttcatgaac gcctaccgcg agagcatcga ctatatctat tcgtcaaacc cgcaggtgct
     3301 gaaagactat gcggagtttg cgcgaatttc ggaacctttg gccaaacgcg tgcgcgacga
     3361 attcttcccc aagagcctgg tcaatccgga tcaaattcac ggtctcgaca cattgattcc
     3421 ggaggcggtg aacctgaaat tcatctccgc tcctctcaca aaggagcagc tcacggaact
     3481 gatccagatt tcgccgcgca agtgagccgc aacagacagg tgcctgcatg accaccgcag
     3541 tttccccgca agccgcttcc gcgcaagcgg tcgtattgcc gaaaaaccgc ggcgcctatt
     3601 acggcggcgc ctggcatgac ccaaaggccg gccgtttcgt cgacacgatc agcccgggca
     3661 cggggcaatc gctcggcaag gtcgccgatt ccggcgccga ggacatcgat gcggcggtgg
     3721 cggcggcaaa gatcgcattc gccgaatggc gccgcgtgct gcccctcgaa cgggcaaaaa
     3781 tcctgcgccg gattgccgaa atcctgcgcc agaacgccaa cgagctcgcg atgatcgatg
     3841 ccgccgattg cggcaacccg gtcaaggaaa tggtgagtga cgcgatgatt gccgcagcgc
     3901 agatggaatt cttcgcgggc ttcgtcacgg aaatgaaggg cgcatcgatc ccgatggggc
     3961 cggacgtcgt caacttttcc gtgcgcgagc ccctgggcgt cgtcggccgc attatcccct
     4021 tcaatcaccc attcatgttc tgcgcgggca aatccgccgc tccgcttgcc gccggcaaca
     4081 cggttgtggt gaagccaccg gaacaggcac cgctgtcatc gctgcgcctg gccgagctga
     4141 tcggcggctt gctgcctacg ggcgccttca acgtggtgcc gggcgggcgc gaggccgggc
     4201 agaggctcgc cagccatccg gacgtcgcga tgatcgcatt gatcggcagc gtgccgaccg
     4261 gccgcgcggt catgaaagcc gcttccgaca cggtcaagcg cacgatgctg gagcttggcg
     4321 gcaagaacgc gctgatcgcc tacggcgatg ccgaccccga cgaggtcgcc ggcgccgtca
     4381 tcggcggcat gaatttcacc tggtgcggcc agtcctgcgg ctccaccagc cgcgccttca
     4441 tccacgagac gatctacgac gccgtggtcg cgcgggtaaa ggagaagatc aaatattaca
     4501 ggccgggcat tccgaccgat cccgccacca ccatgggcgc gatcgtatcc aggcagcagt
     4561 tcgaccgggt catgagtttt atcgactcgg ccaaaagcga aggcgcgcag ctcatcaccg
     4621 gcggcggacc gccggccgat ccggctctca agggcggctt ctatatcgag ccgacagtgt
     4681 tcgccgtcac cgacaagaac aggatcgcgc gcgaggaaat tttcgggccc gtgctaggcg
     4741 tcttcaaatg gagcgacgag agcaagatgc tcgcaaccgt gaacgctgtc gaatacggcc
     4801 tcaccgcatc gatctggacc aacgatctct cgaccgctca ccgcacggcg cttgcggtcc
     4861 aggcgggcta tgtctggatc aacgaggtca gcaagcactt cctcggcgcg cccttcggcg
     4921 gcttcaagca gtcgggcctc gggcgcgagg aatgcttcga ggagatgctc gccttcacgc
     4981 aggaaaagaa tatccacgtg aagctgaagg cgccgagagc ctagaccgtt gttctcataa
     5041 atccggctta tcgagcgcgg ctggtcggat gtcaaaagcg gacatgacgc gactatctac
     5101 tttggaattg agtgatgtta ggcgcgggcg gatttggata ttgcctaacc gatttcaggt
     5161 attcctcgac caccttgatc gccggttcag tagcgaaggg aaacattcca aagacgttga
     5221 gatcttcgtg tgggtccatc tcgatgttga aaagcttcgg gtagccggcc atgggggcgc
     5281 tcgccgatcc tgtgccaggc tgacgctgtg gaccaatgcc ggtcgggtac acgtcggtga
     5341 agtatacgcg ccactgcttc caccgcgccg ccaccaggtc gccgccgata aagctaagca
     5401 ggctgtcacg cttcccggcc tcgcttttgc cgagcagcac gtcggtctga tcaacgccgt
     5461 caataggacg gtcgctcggc atcttggccc cggtgatgct ggcaaaggtt gggaagaagt
     5521 ccatgattga gaacatcgcg taggatgtcg tgtccggctt gatgtgcccg ggccatcgga
     5581 tgaaacagaa ggtccggatc gacccctcgg tgacttcgcc aagctcgcca cggaatggac
     5641 ccgcgctccc catgtctggc gtgcccgcgt tgccatattc gcgaaacgct tccccggaag
     5701 gtccgttgtc ggacgcgaat acgagcagcg tgttgtcatc gacgccgagc tgcttcaggg
     5761 cgtcaaggat ctggccgac
//
LOCUS       DASSRL010000571         6310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_312748, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000571 DASSRL010000000
VERSION     DASSRL010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312748"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFL49_11585"
     CDS             complement(<1..544)
                     /locus_tag="VFL49_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7912665.1"
                     /translation="MRFDFTDLNLFRHIVEAGSITHGAARSNLALAAASTRVRKMELA
                     FGTPLLVRGRAGIKPTPAGRTLLQHARGILAQTERLQEDLSPYARGLAGQVRVLSNTN
                     ALTEFLPETLSSFLMAYPDISVDLEERLSDEIVGLIAEGHADIGIVAGTVDYGGLNTF
                     PFRSDRFVLVVAKNHPLANRS"
     gene            744..1169
                     /gene="sdhC"
                     /locus_tag="VFL49_11590"
     CDS             744..1169
                     /gene="sdhC"
                     /locus_tag="VFL49_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET7912666.1"
                     /translation="MAEFEAGTAGAERPRPLSPHLQIYKPMLTMVMSIVHRITGAALY
                     FGMLLLAWWLIAAASGPNAYAGFEWFMGSVIGQFMLFGYTWALIHHMLGGIRHLIWDT
                     LHGFEPAEREMLTLATLIGSITLTLILWVVGYLVMGGPR"
     gene            1166..1558
                     /gene="sdhD"
                     /locus_tag="VFL49_11595"
     CDS             1166..1558
                     /gene="sdhD"
                     /locus_tag="VFL49_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HET7912667.1"
                     /translation="MRDAPQMRTDMRLVRGLGAAHSGTGHFWHQRITSVAGILLTIGL
                     LLIITALLGRSHAAVVQILASPIVSIIVLLFVINTAYHMWIGMQEIILDYVHDETWKL
                     LSLMANTFFVFAVSLVSIYAVLKLSFGV"
     gene            1560..3389
                     /gene="sdhA"
                     /locus_tag="VFL49_11600"
     CDS             1560..3389
                     /gene="sdhA"
                     /locus_tag="VFL49_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET7912668.1"
                     /translation="MATNGQANGKPAINGRAYPIEDHSYDVVVVGAGGSGLRAVVGCS
                     EAGLRTACITKVFPTRSHTVAAQGGIAAALGNMGEDDWRWHMYDTVKGADWLGDQDAI
                     EYLCRNAPAAVYELEHWGLPFSRREEDGKIYQRPFGGMTTHYGKGTAQRTCAAADRTG
                     HAMLHTMYGQALRHAAEFYVEYFAIDLIMDEGRCRGVVALNMDDGSIHRFAANMTILA
                     TGGYGRTYFSCTSAHICTGDGNAMVLRAGLPLQDMEFVQFHPTGIYPAGCLITEGSRG
                     EGGYLVNSEGERFMERYAPSAKDLASRDVVSRAMTVEIREGRGVGKNQDHLFLHLDHL
                     DPAVLHERLPGISETARIFANVDVTREPIPVLPTVHYNMGGIATNYHGEALTKSNGAD
                     DAVIPGLMALGEAACVSVHGANRLGSNSLIDLVVFGRAAALRCAEILTPGEEQSQLPK
                     DSADLALSRLDKFRNASGGTPTAKLRLNMQRVMQEDCAVFRTGEVLDQGHKRIHEIHD
                     GIVDVHVTDRSLIWNSDLIETLELDNLIAQAVVTMDSARNRTESRGAHAREDYPERDD
                     KNWMKHTLAWMDANGAVKIDYRPVHAYTLTNDVAYIEPKARVY"
     gene            3402..4181
                     /locus_tag="VFL49_11605"
     CDS             3402..4181
                     /locus_tag="VFL49_11605"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET7912669.1"
                     /translation="MVEFTLPRNSRITEGRRWPRSAHATHVTEFRIYRWNPDDGQNPS
                     VDTYYIDRDDCGPMVLDALIWIKNTIDPTLTFRRSCREGVCGSCAMNIDGTNTLACTK
                     AIDDARAPIKIYPLPHQPVVKDLVPDLKNFYAQYASIEPWLQTSTPTPEKEWRQSHED
                     RAKLDGMYECILCACCSTACPSYWWNSDRYLGPAALLQANRWVQDSRDEATGSRLDNI
                     EDPFRLYRCHTIMNCSKACPKGLNPAKEIATLKKALVDRRL"
     gene            4467..5504
                     /locus_tag="VFL49_11610"
     CDS             4467..5504
                     /locus_tag="VFL49_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7912670.1"
                     /translation="MPAAEIEAEQRKQQITQLRTYFTELSRLDNVAFRVRVANRDDCK
                     DRAWAQIGLIAGTVPSLPRKYRAFSHEALSVSWTRATVLSVADSSPAAAAGIKPGDQL
                     LTFNNEPVPRHGTAGWIGGYVRNNGERPIGVLVRRDGTDEMHTIVPVIACAIPIELQV
                     DSSVNAFTTDDGITVSTSIMRAARTDAQLALVVGHELAHANLGHLGKQRANAVIGWIG
                     GAALDAGVMLGGMSTRGTFSRVFTQAGARAFSVAFEREADYVGAYYAARAGYDLAGSE
                     EIWRTFSLESPDSIRITTDHPITPVRFVQMQKVIDEIEDKKRRNLPLVPEPRFIRADA
                     DESGAANETLP"
     gene            complement(5523..5942)
                     /locus_tag="VFL49_11615"
     CDS             complement(5523..5942)
                     /locus_tag="VFL49_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912671.1"
                     /translation="MAKIAANACLLLTLVVGLIGCSGSSSKQPEENPNIFPTDYKNEI
                     LNTMTTTLDNPTNVRGAFISEPVLRPAGKDERYVICVRSDSRNANGNYTGSKDRIGYF
                     YAGHLNQLVDATQEQCGNAAYKPFPELEKLCQAKKCM"
     gene            complement(5958..>6310)
                     /locus_tag="VFL49_11620"
     CDS             complement(5958..>6310)
                     /locus_tag="VFL49_11620"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490473.1"
                     /note="catalyzes the formation of
                     2-N(omega)-(L-arginino)succinate from L-citrulline and
                     L-aspartate in arginine biosynthesis, AMP-forming; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7912672.1"
                     /translation="ESITLDRGAAHLKDELMPKDAELVYYGFWFSPEREMLQAAINRS
                     QEFVTGRVKLRLYKGSVAVIGRESKYSLYDQDLVTFEEGAVAYDHRDAEGFIKLNALR
                     LRTLGQRRRKFKLD"
BASE COUNT         1244 a         1957 c         1877 g         1232 t
ORIGIN      
        1 gcgagcgatt ggcgagtgga tgattctttg cgaccaccag cacgaagcgg tcgcttcgga
       61 acggaaaagt attgagcccg ccgtagtcga ccgtgccggc gacgattccg atgtcggcgt
      121 ggccttcggc aatcagaccg acgatctcat cgctcaaccg ttcttccaga tccacgctga
      181 tgtccgggta ggccatcagg aacgagctca gcgtttcggg aaggaattcg gtgagggcgt
      241 tcgtatttga gagcacgcga acctggccag cgagacccct ggcataaggc gagaggtcct
      301 cctgcaagcg ctcggtttgc gccaggatcc cacgcgcgtg ctgcagcagc gtgcgtcccg
      361 cgggggtggg tttgatcccc gctcggccac gcaccagcag aggggtaccg aacgccagtt
      421 ccatcttgcg gacgcgggtc gaggccgcgg ccaaggccag gttcgagcgc gccgcaccgt
      481 gcgtgatgct gccggcttcg acaatgtgcc ggaacaaatt caagtcggtg aaatcgaagc
      541 gcatagagaa ctaccttctt atttcgcgaa ggcagactac acataaagca tattgcaacg
      601 gactattttc gggtccaagc ttcgccaatc atgaagtatc gacttggggg tccaggtcaa
      661 gaggaatgtt tgcacacccc cacttgcgac cgctgggtct cgtttcacgc aatgcaccgg
      721 tcgcgggttt tcggaggctg acaatggccg agtttgaggc gggaacagcg ggggcggagc
      781 ggccacgccc gctctcgccg cacctgcaga tctacaagcc gatgctgacg atggtgatgt
      841 ccatcgtcca ccggatcact ggtgccgcgc tctacttcgg gatgctgctc cttgcctggt
      901 ggctgatcgc cgccgcaagc ggaccgaacg cctatgccgg ctttgaatgg ttcatgggaa
      961 gcgtgatcgg ccagttcatg ctgttcggtt acacctgggc cttgatacac cacatgcttg
     1021 gcggcattcg ccacctgatc tgggacacgc tgcacggctt tgagccggcc gagcgcgaaa
     1081 tgctcacgct ggccacgttg atcggctcga taacgcttac gcttatcctc tgggtcgtcg
     1141 gctacctcgt catgggaggt ccgcgatgag agacgctccg caaatgcgaa ctgacatgcg
     1201 cctcgtacgc ggattaggcg cggcccattc cggcaccgga catttctggc accagcgcat
     1261 cacgtcggtt gccggcatcc tcctgacgat cggattgctg ctcatcatta ccgcattgct
     1321 cgggcgcagc cacgccgcgg tcgtccagat tctcgcctca ccaatcgtct cgatcatcgt
     1381 gctgctgttc gtcatcaaca ccgcctacca catgtggatc ggcatgcagg aaattattct
     1441 cgactatgtg catgacgaga cgtggaagtt gctgagcctg atggcaaata cattcttcgt
     1501 cttcgccgtc agcctggtgt cgatctatgc cgtcctgaag ctctcgttcg gagtctgaca
     1561 tggcgacgaa tggacaagcg aacggcaagc cggcgatcaa cggacgcgcc tatccgatcg
     1621 aggatcacag ctatgatgtc gttgtggtcg gcgccggcgg ctccggcctg cgcgccgtgg
     1681 tcggctgcag cgaggccggg ttgcgcacgg cctgcatcac caaggtcttc ccgacgcgat
     1741 cacataccgt cgcagcgcaa ggcggcatcg ccgccgcgct cggcaacatg ggcgaggacg
     1801 actggcgttg gcatatgtat gacacggtca agggtgccga ctggctcggc gaccaggacg
     1861 ccatcgaata tctctgccgc aatgcgccag ccgccgtgta tgagctggag cactggggcc
     1921 tgccgttctc gcgccgcgag gaagacggca agatttatca gcggcccttc ggcggtatga
     1981 ccacgcatta cggcaagggc accgcgcagc gcacctgcgc cgccgccgac cgtaccggcc
     2041 acgccatgct gcacacgatg tacggccagg cgctgcgtca tgcggcggag ttctacgtcg
     2101 aatatttcgc catcgatctc atcatggacg aaggccgttg ccgtggcgtg gttgcgttga
     2161 acatggatga cggctcgatt caccgtttcg ctgccaacat gacgatcctt gccaccggcg
     2221 gctatggacg gacatacttc tcgtgcacgt cggcacacat ctgcaccggc gacggcaatg
     2281 ccatggtgct gcgcgccggc ctcccgctgc aggacatgga attcgtgcag tttcatccga
     2341 ccggcattta cccggcgggc tgcctgatca ccgaaggctc gcgtggagaa ggtggctacc
     2401 tggtgaattc cgaaggcgag cgcttcatgg agcgttacgc accgtcggca aaggacctcg
     2461 cctcgcgcga cgtcgtctcg cgggccatga cggtcgaaat tcgcgagggc cgcggtgtcg
     2521 gcaaaaacca ggatcacctt ttcctgcacc tcgaccacct cgatccggca gtgctgcacg
     2581 agcgcctgcc aggcatcagc gaaaccgcgc gcatttttgc caatgtcgat gtgacgcgcg
     2641 agccgatccc cgttttgccg acggtccact acaacatggg cggcatcgcc acgaattatc
     2701 acggcgaagc tttgaccaaa agcaacggcg cggatgatgc ggtcattccg ggtctgatgg
     2761 cgctcggcga agccgcctgc gtgtccgtgc acggcgccaa caggcttggc tccaactccc
     2821 tgatcgatct ggtcgttttc ggccgtgcgg cggcgctgcg ctgcgccgaa atcctcacgc
     2881 ctggcgagga acaatcgcaa ttgcccaagg attcggcgga cctcgcgctg tcgcgactcg
     2941 acaaattccg caacgcatct ggcggcacac cgaccgccaa gctgcgactc aacatgcagc
     3001 gcgtgatgca ggaggattgc gcggtctttc gtacaggcga ggtcctggac cagggccaca
     3061 agcgcatcca tgaaatccat gacgggattg tcgatgtcca tgtcactgac cgttcgctga
     3121 tctggaattc ggacctgatc gagacgttgg agctcgacaa tctgatcgcg caggccgtcg
     3181 tcacgatgga ttcggcccgt aaccggactg aaagccgcgg cgcgcacgcg cgcgaagact
     3241 atccggagcg cgatgacaaa aactggatga aacacacgct ggcgtggatg gatgcgaacg
     3301 gcgcggtgaa aatcgattac cgcccggtgc acgcctacac gctcacgaac gacgtcgcct
     3361 atatcgagcc caaagcgcgc gtctactgac ggaacattgc catggtcgaa tttacgctac
     3421 cccgaaactc cagaatcacc gaaggcagga gatggccgcg ctcggcgcat gctacccacg
     3481 taaccgagtt ccgcatctac cggtggaatc cggacgacgg ccaaaatccg agtgtcgaca
     3541 cctattacat cgatcgggat gactgcgggc caatggttct cgatgcactc atctggatca
     3601 agaatacgat cgatcccact ctcacgttcc ggcgctcatg ccgcgagggc gtatgcggct
     3661 cctgcgccat gaatatcgat ggaaccaaca cgcttgcctg taccaaggcg atcgacgatg
     3721 cgcgcgcgcc gataaaaatc tatccgctgc cgcatcagcc ggtggtgaag gacctggtcc
     3781 ccgacctcaa gaacttctac gcgcaatatg cttcgatcga gccgtggctg cagacgtcga
     3841 cgcccacgcc ggaaaaagag tggcggcagt cgcacgaaga ccgcgccaag ctcgacggca
     3901 tgtacgaatg catcctgtgc gcctgctgct cgaccgcatg tccgagctac tggtggaatt
     3961 ccgatcgcta tctcggcccc gccgctttgc tgcaggcaaa ccgctgggtg caggatagcc
     4021 gcgacgaggc gaccggctcc cgtctcgaca acatcgaaga tccgttccgg ctctaccgtt
     4081 gccatacgat catgaattgc tcaaaggctt gcccaaaagg cctcaacccc gcgaaagaaa
     4141 ttgccacgct gaagaaagcg ctggttgatc ggcggcttta gcggctccct tcgcactgca
     4201 agccgacacg tcggctgtcc gatcgcgccg agaaatcgcg aactatctgt tgtcagcgac
     4261 attcaacgcc tttcggctcg accatacgga cgaccagcat tgaacgcact ctttcgacct
     4321 catacgaacg ccgcttgaga ccataacgaa ggtcatgcag ggtccacgcg gtttgcgagg
     4381 tctaatgcgt gcgcagcgct cgttttctac tattttgtgc gtttttcgcg tgcgccgcat
     4441 gcgccccagt ctcgaagttg ccatcgttgc cggccgctga aatcgaagct gagcagcgca
     4501 agcaacagat tacccagctg cgcacctact tcacggagct tagccgtctc gacaatgtcg
     4561 ccttccgggt gcgcgtcgcc aatcgcgacg actgcaagga tcgggcgtgg gcgcagatcg
     4621 gactcatcgc cggcaccgtc ccgagcttgc cgcgcaagta ccgcgcattc tctcatgaag
     4681 ctttatcggt gagctggacg cgtgcaacgg tactttcggt cgcggacagt tcgccggccg
     4741 cagccgcggg catcaagccc ggggaccagc ttttgacctt caataatgag ccggtcccgc
     4801 gtcacggcac agccggctgg atcggtggct atgtccgaaa taatggcgaa cggcctatcg
     4861 gagtcctggt ccggcgcgat ggcacggacg agatgcacac aattgtgccg gtgattgcct
     4921 gcgcgattcc cattgaactt caggtcgatt ccagcgtgaa tgcatttacg accgacgatg
     4981 gcatcacggt ctctaccagt ataatgcgcg ctgcacgcac cgatgcacag ctcgcactcg
     5041 ttgtcggtca cgagctggcg catgccaatc tcggccacct gggcaagcag cgggcgaacg
     5101 ccgtcatcgg ctggatcggc ggcgccgcgc tggacgctgg tgtcatgctt ggaggaatgt
     5161 ccacgcgcgg caccttttcc agggtgttca cccaggccgg tgcacgggct ttcagcgtcg
     5221 cattcgagcg cgaagccgat tatgtcggcg cctactatgc cgcacgcgct ggctacgacc
     5281 ttgcggggtc agaagaaatc tggcgcacgt tctcgctgga aagtcccgac agcatccgca
     5341 tcacgacgga tcatccgatt acgcccgtgc gctttgtcca gatgcagaag gttatcgacg
     5401 agatcgaaga caagaagcgc cgcaacctgc ccctggttcc ggagccacgc ttcattcgtg
     5461 ctgatgctga tgagtccgga gccgccaacg aaaccttgcc ctgagcagcg ggcggacgac
     5521 cgttacatac acttcttggc ctggcagagc ttttcaagct cgggaaaagg cttgtaggcg
     5581 gcattaccgc attgctcttg ggttgcatcg accagctggt taaggtggcc ggcgtaaaaa
     5641 tagccgatcc gatccttgct tcccgtgtag ttcccgttgg cattgcgcga gtccgagcgc
     5701 acgcaaataa cgtagcgctc gtccttgccc gccgggcgca acaccggctc actgatgaag
     5761 gcaccacgga cgttggtcgg attgtcgagc gtcgtggtca tcgtgttgag aatttcgttc
     5821 ttgtagtcag ttggaaagat gttgggattc tcctccggct gcttgctgct gctgccggag
     5881 caaccgatca aacccactac aagagtcaaa agcaggcagg cgttcgccgc aattttcgcc
     5941 atcggcttct ccgccgccta gtcgagcttg aactttcgcc gccgctgacc gagggtacga
     6001 agccgcaatg cattgagctt gatgaagccc tcggcgtcgc ggtgatcgta ggcgacggca
     6061 ccctcctcga acgtcaccag atcctggtca tagagcgaat acttgctctc gcggccgatc
     6121 accgcaacgc tgcccttgta aagccgcagc ttgacgcggc cggtgacgaa ttcctgcgac
     6181 cggttgatcg ccgcctgcag catctcgcgc tcgggcgaga accagaagcc gtaatagacg
     6241 agctcggcgt ctttcggcat cagctcgtcc ttgagatgag ccgcgccgcg gtcgagcgtg
     6301 atcgactcga
//
LOCUS       DASSRL010000572         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_225131, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000572 DASSRL010000000
VERSION     DASSRL010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225131"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VFL49_11625"
     CDS             <1..1334
                     /locus_tag="VFL49_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7912673.1"
                     /translation="LVNTYVPYGEQPAMTGDPETVRRMAHTGAPVVSNLFTSLVVKKP
                     VFNVSIPVLRDGELRFVMSLGLLPDDLISLLDDQKLGPEWVTMIWDTSGVILARSREN
                     ARYLGSPLPKNMREQERQAIVRTTNLEGIDVLHATARSRISDWGVGVNVPYSLIAEQM
                     RQSLLLWGTAAALAITIALALGLRFARQITTSLSVASEAAAAFGHGEPFPITGSRLKE
                     ADAFLATLRNAQQELSEAQSHQKFLLRELQHRTQNLFAVIQAIIARSLTEEQSVAQAK
                     QLITGRLQALARAHAILTGAAWEGAPLREILKREFGEDFSGYVNESGCDIVVNAGAAH
                     QFALIIHELATNAIKYGALSAPGGSISIVGNIEQANGTPPRFSLSWSESGGPPVVQPT
                     RKGFGSVVLFDAARQFGMDIDVKYDPEGLNYELHVPMSEILPSKAPGGALV"
     gene            complement(1331..2074)
                     /locus_tag="VFL49_11630"
     CDS             complement(1331..2074)
                     /locus_tag="VFL49_11630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET7912674.1"
                     /translation="MSRLLTVVLLLLASPAVALTGNAPPATGWAARAIVMIVDAREDL
                     CTGTALAPDLVLTAAHCVARNIDYQVKAYQTGVAVPVRTIARHPRFDYASYAASRATA
                     DVALIKLAAPLPDAVTPASLAAPRRVAPGETLTIAGFGVTLAGTARGLGQPRMATLTV
                     TGKPGSLQIRLYDVATRNQRIGLGSCTGDSGAPAYDGEGPLVIGVVSWSTAPGDEEGC
                     GGLTGVTPLLSYRDWIIDTARKLNSPIVP"
     gene            2250..2450
                     /locus_tag="VFL49_11635"
     CDS             2250..2450
                     /locus_tag="VFL49_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912675.1"
                     /translation="MSPAERYRTLAAHLRTRAARETNRALRTQWANLAQCYVRLAEQA
                     DLNTRADIVYEFDRSTTGRVGG"
     gene            complement(2491..2937)
                     /locus_tag="VFL49_11640"
     CDS             complement(2491..2937)
                     /locus_tag="VFL49_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET7912676.1"
                     /translation="MPNPRSTAQIAGHPIHPMLIPFPIAFFVSTFVADLVYWQTGSAA
                     WANATLWMLGAGLVMAALAAIAGLTDVLGDVQIRNLSDAWLHAGGNVVVVLIQLYNWY
                     SRYQHGGDAILPTGLVLSLIVVLILLFTGWKGWEMVYRYRVGVADG"
BASE COUNT          659 a          983 c         1040 g          560 t
ORIGIN      
        1 aactggtcaa tacctatgtg ccatatggcg aacagccggc catgaccggc gatccggaaa
       61 ccgtccgccg catggcgcac actggggcgc cggtggtctc caatctcttc acgagcctgg
      121 tggtgaagaa accggtgttc aacgtctcaa taccggttct gcgcgatggc gagctccgat
      181 ttgtcatgag cctcggcctg ctccctgatg acctgatttc gttgctcgac gatcaaaagc
      241 tggggccgga atgggtgaca atgatctggg acaccagcgg ggtcatcctg gcacgttcac
      301 gcgagaacgc gcgttacctc ggctcaccct tgccaaaaaa tatgcgcgag caggagcggc
      361 aagccattgt caggacaacc aacctggagg gcatcgacgt gctgcatgca acggcgcgat
      421 cgcggatttc agactggggt gtaggggtca acgtcccgta ctcgttgatc gccgaacaga
      481 tgcggcaatc attgcttctg tggggtacgg cggcagcgct cgcgatcacc attgcgcttg
      541 cattgggcct gcgcttcgcg cgccagatca cgacgtctct gtcggtcgcc tccgaagctg
      601 ctgccgcctt tggtcacggt gaaccgtttc caattacggg atcgcgtctc aaggaggccg
      661 atgcgttcct ggccactctg agaaacgccc agcaggagct gtcggaagcg cagagccatc
      721 aaaaatttct gctgcgggaa ttgcagcacc gcacgcagaa cctgtttgcg gttattcaag
      781 ccatcattgc ccgcagtctc accgaggagc aatcagttgc gcaggcgaaa cagctgataa
      841 cgggtcgcct gcaagcactg gcacgcgctc atgcaatcct taccggggca gcctgggaag
      901 gcgcaccgct ccgcgagatt cttaaacggg aattcggaga ggatttttcc ggctatgtga
      961 acgaaagcgg ttgcgacatc gtcgtcaacg caggcgccgc ccatcaattc gccctcatta
     1021 ttcacgagct tgcgaccaac gccatcaagt acggagcgct gtccgcgccg ggcgggagta
     1081 tctcgatcgt gggcaatatc gagcaggcca atggaacgcc gcccaggttc tcgctctcgt
     1141 ggagcgagag cggcgggccg ccggtcgtac agccgacgcg gaagggattt ggcagcgtcg
     1201 ttctgttcga tgcggccagg cagttcggaa tggacattga cgtgaagtac gatccggaag
     1261 gcttgaatta cgagctgcat gttcccatga gcgaaatact gccatcgaag gcgccgggtg
     1321 gcgcgctggt ttaaggcacg atcggcgaat tgagtttgcg cgcggtgtcg atgatccagt
     1381 cgcggtagct cagaagcggc gttactccgg tgaggccgcc gcaaccttcc tcatcgccgg
     1441 gcgcggtcga ccaactcacc acgccgatca ccagcggacc ttcgccgtca taggccggcg
     1501 cgccggaatc gccggtgcag ctgccgaggc cgatgcgctg gttgcgcgtc gcaacgtcgt
     1561 aaagacggat ctgcaacgag ccgggtttgc cggtaacggt gagcgtggcc atgcgcggct
     1621 ggccaagccc gcgcgcggtt cccgccagcg tgacgccgaa gccggcgatg gtcagcgtct
     1681 cgccgggggc gacacggcgc ggcgcggcga gcgaagccgg agtcacggca tcggggagcg
     1741 gcgccgcgag cttgatgagc gcgacgtcgg cggtggcgcg gctcgcggca tagcttgcat
     1801 agtcgaagcg cggatggcgc gcgatagttc gcaccggaac ggcgacgccg gtctgatagg
     1861 ccttgacctg gtaatcgata ttgcgcgcaa cgcaatgcgc ggccgtgagc acaagatcgg
     1921 gcgcgagggc tgtgccggtg cacaggtcct cgcgcgcatc gacgatcata acgatggcgc
     1981 gggccgccca gccggtcgcc ggcggcgcat tgccggtgag cgctacagcg ggggaggcaa
     2041 gcagcaggag gacgacggtc agaaggcggg acatgccccg tgcatgagcc ccggcggcgg
     2101 aactttgtca acgccgaagg aaattatggc agcgggaacc ctaagtcgct gaatgcgcga
     2161 tcaaaaacct gggaattttt tgtgacgcgc ggaacggctg gccgcgtgat tggtttgtga
     2221 aagacatact ttaggaagca acgccaatca tgagtcccgc cgaacgatac cgaacactcg
     2281 ccgcgcacct gcgtacccgc gcggcgcggg aaacgaaccg cgcgttgcgc acacaatggg
     2341 cgaacctggc gcaatgctac gtacggctcg ccgagcaagc ggatctgaac acccgggccg
     2401 acatcgtcta cgagttcgat cgctcgacaa ctggcagggt cggcggctag ccggcccgac
     2461 cgcttcgaag gcgcggggcg aactcagcca tcagccgtcc gcgacgccca cccggtagcg
     2521 gtaaaccatt tcccagcctt tccagccggt gaatagcagg atcagcacca cgatcagcga
     2581 gaggacgagg ccggtcggca ggatcgcatc gccgccgtgc tgatagcgtg aataccaatt
     2641 gtagagctgg ataagaacga cgacgacatt gccgccggcg tgcagccacg cgtccgacag
     2701 gtttcgaatc tgcacatcgc ccagcacgtc ggtgagcccg gcaatggccg caagtgccgc
     2761 catcacaagg ccggctccga gcatccacag agtcgcattg gcccaggcgg ccgagcctgt
     2821 ctgccaatac acaagatcgg ccacgaaggt cgacacgaag aaagcaatcg ggaatggaat
     2881 cagcatcgga tggatgggat ggcccgcaat ctgggccgtc gatcgggggt tcggcattgt
     2941 ttcctccaaa agcgacaaag aatgccgaag caactgtttg gtttggtcgc cggttccacc
     3001 tgatcagccg gtcgtgcgcc tgatcgttca atcacgtaca tcgcttgcgc cgaccaggga
     3061 ttcgccgggc acgtcacgcg caacggcaat gcaatctgca cactatgtcg gacatcacac
     3121 atatgtcatc catacttctc gcgccgcctg cggccgagcc gctatcgctc gccgaggcga
     3181 aagtctttct gcgcgtcgag accgccgacg aggatccgct gatcgccgct ttgatcacgg
     3241 cg
//
LOCUS       DASSRL010000573         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1064989, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000573 DASSRL010000000
VERSION     DASSRL010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064989"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFL49_11645"
     CDS             complement(<1..737)
                     /locus_tag="VFL49_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912677.1"
                     /translation="MSAGAAFRLRAAHAQRAVHAIAMHGEPAWGPTFSHPPYANPQAP
                     KGGHLTQGVLGTFDCLNPFIVKGLPAANIRGYIVESLLARGYDEPFSLYGLLADRVTT
                     DAARTYVTFRINPAARFADGKPVTPADVIFSWELLRDKGRPNFRTYYIKATRAEAIDA
                     RTVRFDLYGADDRELPLIIGLMPVLAKHAVNGATFEDTSFDPLLGSGPYTVGNVRPGE
                     SVTFTRDKNYWGRDLPINRGLWNFDTIR"
     gene            1087..1788
                     /locus_tag="VFL49_11650"
     CDS             1087..1788
                     /locus_tag="VFL49_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HET7912678.1"
                     /translation="MVMTLATTMFAGTAYAQQQPKAGTKPAPKAQPAQPLQPAQPQQQ
                     AGDQPQLMYSPWMKVCGQGQDTNNKKVCVITKDGRLENGMPVAIVQLFEPEGEPKVLR
                     VTVPLGMQLAHGTRLIIDQQQPAQQPYKICFPVGCMADYPVTDDMIAKMKKGQNIVVQ
                     AINMQGTPISLPLPLGDFAKAYDGPPTDPKAFEEQQRKLQEELQKKAEEARKKLEGQQ
                     SQAPAAPAPAPAQKK"
     gene            complement(1853..2185)
                     /gene="hspQ"
                     /locus_tag="VFL49_11655"
     CDS             complement(1853..2185)
                     /gene="hspQ"
                     /locus_tag="VFL49_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HET7912679.1"
                     /translation="MDKMRQAKFKIGQVVKHRVHPFRGVIFDIDPVFNNTEEWLQSIP
                     ADVRPHKDQPFYHLFAENAETEYIAYVSEQNLLPDTSGKPVRHPDVAEAFVRDDSGDY
                     RPRNSQIH"
     gene            complement(2206..>2765)
                     /locus_tag="VFL49_11660"
     CDS             complement(2206..>2765)
                     /locus_tag="VFL49_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7912680.1"
                     /translation="NNVEAIATASPRMHGMSLGPADLAASRAMKTTRVGGGHPDYKVL
                     ADAQGDAPRASSQQDLWHYTIAKMVDACAAAGIKPFYGPFGDFADPDACEAQFRNAFL
                     MGCVGAWSLHPSQIDIAKSVFSPDPGEVAFARKIVAAMPDGAGAVMIDGKMQDDATWK
                     QAKVLLDLAKLVAARDPDAGKRYGL"
BASE COUNT          555 a          808 c          908 g          494 t
ORIGIN      
        1 cggatcgtgt cgaagttcca gaggccgcga ttgatcggca gatcgcgacc ccagtagttc
       61 ttgtcacgcg tgaaggtgac gctttcgccc ggcctgacgt tgccgacggt gtacggcccg
      121 ctgccgagca acggatcgaa gctggtgtct tcgaaagtcg caccattgac ggcgtgtttt
      181 gccagcaccg gcatcagccc gatgatgagc ggcaattcgc ggtcgtcggc gccgtacaga
      241 tcgaagcgca cggtgcgcgc gtcgatagct tcggccctcg tggccttgat gtaataagtc
      301 cgaaagttgg gacggccttt gtcacgcagt aactcccagg agaaaatcac atcggccggg
      361 gttaccggct tgccatcggc gaagcgagcc gccggattga ttctgaaagt gacgtaggtt
      421 cgcgcggcgt ctgtcgtgac gcgatcggcc agaaggccgt agaggctgaa tggctcatcg
      481 tagccgcgcg cgaggagact ctcgacgatg tagccccgga tatttgccgc gggcagccct
      541 ttcacgatga aagggttgag gcagtcaaag gtgccgagca cgccttgtgt caggtgcccg
      601 cctttcggtg cctgcggatt ggcgtagggc ggatggctga aagtcggtcc ccaggccggt
      661 tctccgtgca tggcgatggc gtgaacagcg cgctgggcgt gcgccgcgcg cagacgaaag
      721 gcagcgcccg cagacaatgc gccgacgacg atggaacgtc gcgacgggag caaagcggtc
      781 cccgcaaggg cagtcgaggg aggcacgagt gtcgggcgaa tgcgattcgg tccggttttg
      841 caggaattta tcatatggcc gcagggcgag gacgagccgc attttcagcg cattcgtggc
      901 tttattgcag aagggaattc ggctatcagg cgtcacgcta ccgccgtatt tgacccggag
      961 agagccgggc agccagcggc tatttcaggg gcgtcatagg ccgcggacgg aaataacaag
     1021 ggaacaatcg caatgaatca tcggaccgca ccggtccggc cgtggggccg ggcgttgaac
     1081 gccaccatgg tcatgacttt ggccaccacg atgttcgcgg gcacggctta cgcgcagcag
     1141 caacccaagg cgggcacgaa gccggccccg aaggcgcaac cggcgcagcc gctgcaaccg
     1201 gcacagccgc agcagcaggc gggtgaccag ccgcagctga tgtactcgcc atggatgaaa
     1261 gtctgcggcc agggtcagga cacgaacaat aagaaagtct gcgtcatcac caaagacggt
     1321 cgtcttgaaa acggaatgcc cgtcgccatc gtccaattgt tcgagccgga aggcgagccg
     1381 aaggtcctgc gggtcactgt tccgctgggg atgcagctcg cgcacggcac acggctgatc
     1441 atcgaccagc agcagccggc gcaacagccc tacaagattt gctttccggt cggctgcatg
     1501 gcggactatc cggtcaccga cgacatgatc gccaaaatga agaaggggca gaacatcgtc
     1561 gtgcaggcga tcaacatgca gggcacgccg atcagcctgc cgctgccgtt gggcgacttt
     1621 gccaaggcct atgacggtcc gccgaccgat ccgaaagcgt tcgaggaaca gcagcgcaag
     1681 ctccaggaag agctgcagaa gaaggcagaa gaagcgcgca agaaattgga ggggcagcag
     1741 agccaggcac ctgccgcgcc ggctcccgct cccgcgcaaa agaagtagcg acaccgtcat
     1801 tacaaaaaag gcgcggcagg acccgcgcct tttttatgtc gagccggcgg gctcaatgta
     1861 tctgggaatt gcgcggccgg taatcgccgc tgtcgtcgcg cacgaaagcc tccgcgacgt
     1921 cggggtgacg gaccggtttt cctgaagtat ccggcaacag gttctgctcg gacacgtagg
     1981 cgatgtactc ggtttcggcg ttctcggcga acaggtgata gaatggctgg tccttgtgcg
     2041 gacgcacgtc agccgggatc gactgcagcc attcctcggt gttgttgaac acgggatcga
     2101 tatcgaaaat tacgccgcgg aaaggatgga cgcggtgttt caccacctgt ccgatcttga
     2161 atttggcctg tcgcatcttg tccattcgaa attctcgata tcgaatcaaa ggccgtagcg
     2221 cttgccggcg tcggggtccc tggcggcaac cagtttcgcc aggtcgagga gtactttcgc
     2281 ctgcttccag gtggcgtcgt cctgcatctt gccatcgatc atgactgcgc cggcgccgtc
     2341 aggcatggcc gcgacgattt tcctggcgaa ggcgacctcg ccgggatcgg gcgaaaatac
     2401 gctcttggcg atgtcgatct gcgacgggtg cagtgaccag gcgccgacac agcccattag
     2461 gaacgcgttg cgaaactggg cctcgcaggc gtccggatcg gcgaagtcgc cgaatggacc
     2521 gtagaacggc ttgatgccgg ccgcggcgca tgcgtcgacc attttcgcga tcgtgtaatg
     2581 ccacaggtcc tgctgcgacg aagcgcgcgg cgcatcccct tgcgcgtccg cgagcacctt
     2641 gtaatcggga tgtccgccac cgacgcgcgt cgtcttcatc gcgcgcgacg ccgcaaggtc
     2701 tgccggtccg agactcatgc catgcatgcg gggggaggcg gtcgcgattg cttcgacatt
     2761 gttga
//
LOCUS       DASSRL010000574         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1467243, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000574 DASSRL010000000
VERSION     DASSRL010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467243"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1147
                     /gene="ispH"
                     /locus_tag="VFL49_11665"
                     /gene_synonym="lytB"
     CDS             173..1147
                     /gene="ispH"
                     /locus_tag="VFL49_11665"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7912681.1"
                     /translation="MSANPPKPALKVLLCSPRGFCAGVVRAIESVERALTKYGAPVYV
                     RHEIVHNRYVVDSLRKKGAIFVEELSEIPDDNVPVIFSAHGVPRAVPEEASRRNLISL
                     DATCPLVTKVHREAEIHFRRGREIVLIGHAGHPEVVGTMGQLPSGSITLVQTPEDIDR
                     FTPYDESKLAYVTQTTLSVDDTADMVDHLKKRFPNIVGPHKEDICYATTNRQEAVKRV
                     APIVDAMIVVGAPNSSNSQRLKEVAERSGCPRAVLVQRAAELNWNYFDGVAKIGVTAG
                     ASAPEILVEEILGALSERFAIELETVTATEEGVFFPLPRPLREHEPAE"
     gene            1155..2114
                     /locus_tag="VFL49_11670"
     CDS             1155..2114
                     /locus_tag="VFL49_11670"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7912682.1"
                     /translation="MAVYTDVAAEDLARFLAGYDVGELLAYKGIAEGVENSNFLVHTG
                     AGNFILTLYEKRVAEGDLPFFLGLMEHLAARGITCPQPVKNRQGGMLGKIAGRPAAIV
                     TFLDGLWIRRPNPGHCAAVGEALAQLHLAGADFKLRRTNALSIESWRPLYEHAKPRGD
                     SVRPGLCAEIAGELDALEKSWPRRLPQGVIHADLFPDNVFFLGTKLSGLIDFYFACTD
                     TLFYDVAVCLNAWCFEADHSYNVTKGRALLNAYNKVRALSEAERSSLPILARGAAMRF
                     LLTRLVDWLAVPDGALVRPKDPLEYYRKLRFHQSVKSVEDYGL"
     gene            2114..2584
                     /gene="rnhA"
                     /locus_tag="VFL49_11675"
     CDS             2114..2584
                     /gene="rnhA"
                     /locus_tag="VFL49_11675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET7912683.1"
                     /translation="MTEQRVTIHTDGACSGNPGPGGWGAILAFGDHEKELTGGEPHTT
                     NNRMELMAAISALEALRRPCTVDLHTDSEYLKNGITSWIHGWKKNGWRTSDKKPVKNI
                     DLWKRLDEALGHHKVRWHWVKGHAGHAMNERADELAREGLVASRAGATGAMKTN"
     gene            complement(2645..3130)
                     /locus_tag="VFL49_11680"
     CDS             complement(2645..3130)
                     /locus_tag="VFL49_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7912684.1"
                     /translation="MPIKVGDRLPDAKFRVMTTEGPVWKTTDEVFKGRKVALFAVPGA
                     FTPTCNNLHIPSFLNNAAAIKAKGVHTIAVTGVNDVFVMEAWKKSTGAEGKIDFLADG
                     NGEFAKAIDMAFDGSGNGLGTRSKRYSMLVDDGVVKKLNIEDAPGKCEISGGQALLAQ
                     L"
     gene            complement(3197..4036)
                     /locus_tag="VFL49_11685"
     CDS             complement(3197..4036)
                     /locus_tag="VFL49_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HET7912685.1"
                     /translation="MSDIHRSFCAALVSCVIAGLTGTPVHAADESPWNEDVRSAIRLI
                     AGSSKADDATLRAGIEIKLQPGWKTYWRYPGDSGVPPHFDFSGSENLKNVDVLYPAPH
                     LFTDETGQSLGYKDAVTFPLSVSPQQPGKPVKLRVKVDYAVCEKLCVPAEGRAELVLE
                     AGNSKNNPALMAAEGRVPKHVTATQLGLTVKRIGAGTKPSVVVDLGAPAGKPVELFVE
                     GPTPQWALPIPKPVKSAPAGRAQFSFELDGLPPGADAKSPVDLTFTVVTGDRAVETKT
                     HLD"
     gene            4215..4838
                     /locus_tag="VFL49_11690"
     CDS             4215..4838
                     /locus_tag="VFL49_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020628806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET7912686.1"
                     /translation="MKIARKATQKNTAPGRGYLDGQMLIAMPAMSDERFARAVIYVCA
                     HSTEGAMGIIVNQPAQNIKFPDLLVQLEVIPAAERIQLPGRAEDVKVLKGGPVETGRG
                     FVLHSADFFIENSTLPIDEGICLTATLDILKAIARGKGPASAILALGYAGWAPGQLEE
                     EIQQNGWLHCTADPELIFGQDTDMKYEKALRKIGIDLGMLSSEAGHA"
     gene            complement(4845..>6326)
                     /locus_tag="VFL49_11695"
     CDS             complement(4845..>6326)
                     /locus_tag="VFL49_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7912687.1"
                     /translation="TQDFRLRTADGHYLWFTLKARPVVGSDGEVVRLVGTLTDVTDFK
                     NAEERLLFDAVHDNLTGLPNRELFLDRMEAAFGFARADSQIRPSIVVIDLDRFKQVND
                     SVGMAVGDSILLTIARRLSRLLKPQDTLARLSGDQFALIILSEREPARIVAFAETIRR
                     TLRAPIAFNDREIFLTASIGLALAENAAHRTDEVLKDAELAMYYAKRIGGDRIEIFKP
                     AMRSRKTDRLTMESELRRAIEREEIKVLYQPIVRLEDRAIAGFEALARWDHPKLGRLA
                     PAEFISMAEEIGLIVDLGLFVLDHTAKQLAQWQAATRAREPVFASVNVSSRQLLRHDL
                     IQDLRGVLGRTALARGTLKLEITESLVMENPEHAAQMLTRIKELGAGLSLDDFGTGHS
                     SLAYLQRFPFDTIKIDQSFVRTTPRGTRPVILRSIIALAHDLGMEVVAEGAETDSDAV
                     ELYQLGCEYAQGFAFGEPMTPDAARDLLVDKPSRLRALKIASE"
BASE COUNT         1256 a         1855 c         1973 g         1242 t
ORIGIN      
        1 gcttcgcccg ccctccgggc gaagcaccag atcatcgatg atgatcggaa agtggccgga
       61 atataggggg agaggcccgg tcatgcaacc gttccgcttg acaggggagg cccgtgcacc
      121 ccatgtcggg ggttgaaatg cgcgcggaaa tcgcgcggtt tcgaggaatt atatgtcagc
      181 aaacccgcca aagcccgccc tgaaggtcct gctctgttcg ccgcgcgggt tttgcgccgg
      241 agtcgtgcgt gcgattgaat cggtggagcg ggcactcacc aaatatgggg cgccggtcta
      301 cgtgcggcac gagatcgtgc ataaccgcta tgtcgtcgac agcctgcgaa aaaagggcgc
      361 gatcttcgtc gaggagctga gcgagattcc cgatgacaac gtgccggtga ttttctccgc
      421 acatggagtt ccacgcgccg tcccggaaga agcctcgcgg cgcaatctga tttcgctcga
      481 cgcaacgtgt ccgctcgtga ccaaggttca tcgcgaagcg gagatccatt tccggcgcgg
      541 acgcgagatc gtgctgatcg ggcatgccgg ccacccggag gtggtcggca cgatgggcca
      601 gcttccatcc ggaagcatca ctttggtgca aacaccggaa gacatagacc ggtttacgcc
      661 gtacgacgag agcaagcttg cttatgtgac gcaaaccacg ctgtcggtcg acgacaccgc
      721 cgatatggtc gatcatctca agaaacggtt tcccaatatc gtcggtcccc acaaggaaga
      781 catctgctac gccaccacga atcgccagga ggcggtcaag cgggtggcgc cgatcgtgga
      841 tgcgatgatc gtcgttggcg cgccaaattc gtcaaactcg caaagactga aggaagtcgc
      901 cgagcgctcg ggctgcccgc gcgccgttct ggtgcagcgc gcggccgagc tcaactggaa
      961 ttacttcgac ggcgtcgcca agatcggcgt cacggcgggc gcgtcggcgc cggaaattct
     1021 cgtcgaggaa atcctcggtg cgctctcgga gcgcttcgcg atcgaactcg agaccgtcac
     1081 tgcgaccgag gagggagtgt tctttccgct gccccgcccg ctgcgggagc acgagccggc
     1141 ggagtagcgc ttaaatggcg gtctacaccg acgtggcggc ggaagacctt gcccgctttc
     1201 tcgcaggcta tgacgtcggc gagttgctcg cctacaaagg catcgccgag ggtgtcgaga
     1261 actcgaattt tctggtgcat acgggcgccg gaaatttcat tctcacgctg tatgaaaagc
     1321 gcgtcgcgga aggcgacctg ccattctttc tgggcctgat ggagcacctc gctgcgcgcg
     1381 gcattacctg cccgcagccg gtcaaaaaca ggcagggcgg catgctgggc aaaatcgccg
     1441 ggcgaccggc ggcgatcgtg acctttctcg acgggctttg gatccggcgg ccaaatccgg
     1501 ggcactgcgc ggcggtcggc gaagcgctgg cgcaactgca tcttgcaggc gcggacttca
     1561 agctcaggcg gacgaatgcg ctgtcgatcg agagctggcg gcctctttat gagcacgcaa
     1621 aaccgcgcgg cgacagcgta cgccccgggc tttgcgctga aatcgccggt gagctcgacg
     1681 cactggaaaa atcctggccg cgccggttgc cgcagggggt catccacgcc gatctgtttc
     1741 cggacaacgt cttctttctc ggcaccaaat tatccggatt gatcgatttc tattttgcct
     1801 gcaccgatac gctcttttat gacgtggcgg tgtgcctcaa cgcctggtgc ttcgaggccg
     1861 accattccta caatgtcacg aagggccgcg cgttgctgaa cgcatataac aaggtgcgcg
     1921 cactgtccga ggcggaacgg agctccttgc cgattctcgc gcgtggcgcc gccatgcggt
     1981 ttcttctaac gcggctggtt gactggctcg ccgtgcccga cggtgcgctg gttcggccga
     2041 aggacccgct cgaatattac cgcaagctgc gttttcacca gtcggtgaaa agcgtcgagg
     2101 attacggcct ctgatgaccg agcagcgcgt gaccattcac acggatggcg cctgctcggg
     2161 caatccgggc ccgggcggct ggggcgcgat cctcgccttc ggcgatcatg aaaaggagct
     2221 cacaggcggc gagccgcata cgacaaacaa ccgcatggaa ttgatggcgg caatctcggc
     2281 gctcgaagcg ctcaggcggc cgtgcacggt cgacctgcat accgacagcg aatatctcaa
     2341 gaacggaatc acgtcatgga ttcacggctg gaaaaagaac ggctggcgca cgtcagacaa
     2401 gaagccggtc aagaacatcg acctgtggaa gcggctcgac gaggcgctcg gccaccacaa
     2461 ggtgcgctgg cattgggtca agggacatgc cggtcacgcg atgaatgagc gggccgacga
     2521 gcttgcgcgc gaaggccttg ttgcctcgcg tgcaggtgca acgggcgcga tgaagacgaa
     2581 ctgatcctgc aaaacgaaat gaccgggaca attcccggcc atctcgagga ccattgaaga
     2641 aattctagag ctgcgccagc agcgcctggc cgccggaaat ctcgcacttt cccggggcgt
     2701 cctcgatgtt gagctttttg acgacaccgt catcgacaag catcgaataa cgcttggatc
     2761 gcgtgccaag cccgttgccc gacccgtcaa aagccatgtc gattgccttg gcgaattcgc
     2821 cgttgccgtc agcaagaaaa tcgatcttgc cttccgcgcc ggttgatttc ttccaggctt
     2881 ccatgacgaa cacatcattc acgcccgtca ccgcgatggt gtgcacacct ttcgccttga
     2941 tggcagccgc gttattcaga aagctcggta tgtggaggtt gttgcaggtc ggagtgaaag
     3001 cgccgggcac ggcaaacaga gcgaccttgc gtcctttgaa aacctcgtcc gtcgttttcc
     3061 agaccggacc ttcggtcgtc atgacgcgga acttggcatc gggcaatcga tcaccgacct
     3121 tgataggcat gggtatcctc tcgcgttgaa aatagttttg gtttcttgac tgcggtagtt
     3181 acgcccttgt tggcgtttag tcgagatggg ttttcgtctc gaccgcgcga tcgccggtga
     3241 cgacagtaaa cgtcagatca accgggcttt tggcgtcggc gccaggcggc aggccatcga
     3301 gctcgaagct gaattgggcg cggccggcag gcgccgattt caccggtttc ggaattggca
     3361 gagcccattg tggcgttggt ccttcgacaa acagctctac cggtttgccc gcgggtgcgc
     3421 cgaggtcgac gacaaccgat ggcttggttc cggcaccgat ccgcttcacg gtgagcccca
     3481 gctgcgttgc ggtgacgtgc tttgggacgc gcccttcagc cgccatcaag gccggattgt
     3541 tcttgctgtt accggcttca agcaccagtt cggcgcgacc ctcggcgggc acacacagtt
     3601 tctcgcaaac tgcgtagtcg accttcacgc gaagcttgac aggcttcccg ggctgctgcg
     3661 gcgagacgga aagcggaaat gtcaccgcgt ccttgtagcc aagcgactga cctgtctcgt
     3721 cggtgaacag atgcggtgcc gggtagagca cgtcaacgtt cttgaggttt tccgagcctg
     3781 aaaaatcgaa atgcgggggc acgccggaat cgccggggta gcgccaatag gttttccagc
     3841 ctggctgcaa tttgatttcg atgccggcgc gcaatgtggc gtcgtctgct ttgctgctgc
     3901 ccgcaataag cctgattgcg gatcgcacat cctcattcca gggtgattcg tcggccgcgt
     3961 gtaccggcgt cccggtcagg cccgcaatga cgcacgaaac cagtgccgcg caaaatgatc
     4021 tgtgaatatc gctcatcgcc tttggtctag ttggtaacta tatgccgcgc cactgaattc
     4081 attgtgagca tgcgttgaac acgatccggg caggccgccg ccacaattgt ccgatcgaat
     4141 atgacaggaa aggattgaac tttccgctac aagcttcgtt tgcaaaggcg actcgttcgg
     4201 gtaacctttt tgccatgaaa atcgcacgca aagcgacgca gaaaaacaca gcgcccggac
     4261 ggggttatct cgacgggcaa atgctgatcg caatgccggc catgagtgat gagcgcttcg
     4321 cgcgtgccgt gatctatgtt tgtgcccact cgaccgaagg tgcgatgggc atcatcgtca
     4381 accagccggc gcagaatatc aaattccccg atcttcttgt gcaactcgaa gtgattcccg
     4441 ccgccgagcg tatccagctt cccggccgtg ccgaagacgt gaaagtgctc aagggcggtc
     4501 cggtggagac cggacgcgga tttgtcctgc attcggccga tttttttatc gagaattcaa
     4561 cgctgccaat cgatgaagga atttgtctta ccgcgacgct tgatattctc aaggcgattg
     4621 cgcggggcaa agggccggcc agcgcgatcc tcgcgcttgg ttatgcggga tgggcgccag
     4681 ggcagcttga agaagagatc cagcaaaacg gctggctgca ctgcaccgct gatcctgagc
     4741 tgatctttgg gcaggatacc gacatgaaat atgaaaaggc cctgcgcaag atcggcatcg
     4801 atcttggcat gctgtcgagc gaagccggcc acgcctgatt ttgactattc agacgcaatc
     4861 ttgagggcgc gcaggcggct cggcttgtcg acgagcagat cgcgcgcggc gtccggggtc
     4921 attggctcgc cgaatgcgaa accttgcgcg tattcgcaac caagctggta aagctcgact
     4981 gcgtctgaat cggtttcggc gccttccgcg actacttcca ttccaagatc gtgggcaagc
     5041 gcgatgatag accgcaggat aaccggccgg gtgccgcgcg gtgttgtgcg cacgaatgac
     5101 tggtcgatct tgatcgtgtc gaatggaaag cgctgcaggt aggcaagcga ggaatgtccg
     5161 gtgccgaaat catcgagcga caggccggca cccagttcct ttattctggt cagcatctgc
     5221 gcggcatgct ccggattttc catgaccagc gattccgtga tctcgagctt gagcgtcccg
     5281 cgtgcaagcg ccgtgcggcc caggacccca cgcagatcct ggatgagatc gtgacgcagc
     5341 aactggcggg acgatacgtt cacgctggca aagaccggct cgcgcgcgcg agtcgccgcc
     5401 tgccattgcg ccagttgctt ggcggtgtgg tcgagaacga acagtccgag atcgacaatc
     5461 aggccgatct cctcggccat ggaaatgaat tccgctggtg ccaaccgccc gagtttgggg
     5521 tgatcccagc gcgccagcgc ctcaaagcct gcaatggccc ggtcctccag gcgaacgatc
     5581 ggctgataga gcactttgat ctcttcgcgc tcgatggcac ggcgcagttc ggactccatc
     5641 gtcagccggt cggtcttgcg cgagcgcatg gccggcttga agatttcgat gcggtcgccg
     5701 ccaatacgct tggcgtagta catcgcgagc tcggcgtcct tgagcacctc gtcggtccga
     5761 tgcgcggcgt tctcggcaag cgccaatccg atcgatgccg tgaggaatat ctcgcggtcg
     5821 ttgaaggcga tcggcgcgcg cagggtgcgg cggatggttt cagcaaaggc gacgatgcgc
     5881 gcgggctcgc gttccgacag tataatcagg gcaaactggt cgcctgacag tcgcgccagc
     5941 gtgtcctgcg gcttaagcag ccgggacaga cggcgggcga tcgtgagcag gatggaatcg
     6001 cccacggcca tgccgacgga atcgttgacc tgcttgaagc ggtcgagatc gatcaccacg
     6061 atcgaggggc ggatctgaga gtcggcgcgg gcaaagccga aggcggcttc catgcggtcg
     6121 agaaacagct cacggttcgg cagccccgtg aggttgtcgt gaacggcatc gaacagcagc
     6181 cgttcctctg cattcttgaa atcggtcacg tcggtgagcg ttcccaccag gcgcacgact
     6241 tcgccgtcgg agccgaccac gggccgtgcc ttgagcgtga accagagata atggccatca
     6301 gcggtacgca gccgaaaatc ctgggt
//
LOCUS       DASSRL010000575         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_540359, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000575 DASSRL010000000
VERSION     DASSRL010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540359"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFL49_11700"
     CDS             <1..248
                     /locus_tag="VFL49_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7912688.1"
                     /translation="GVGNECNALSGLYHVARKEELPDAKQSALGSRVLYLDKDDYRIH
                     GFEGPIKLSAGCIRLVEDDVKYLYNHTPLSTRVVVAN"
     gene            complement(263..1252)
                     /locus_tag="VFL49_11705"
     CDS             complement(263..1252)
                     /locus_tag="VFL49_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912689.1"
                     /translation="MGVPTMKLKATCALALLMFASVSTQPVPAADYPERNVTVVVPFP
                     PGGASDVTARLTGNKLSERIGKSVVIENRAGANGSIGAAGVKTVTPDGYTLLIGSIGV
                     YAINPVLYKNLKYDPLKDFDLLSLLVRTPNVLVTNPNFPVNNAADLIAHLKKNPNQVT
                     FASSGIGSSDHLTAALFWQKTGTTGVHVPYKGGGPAINDLIGGHASVSFQNLGAVAQH
                     IKAGKLKALAVTSDKRAAALPDVPTATEAGIADLIVYSWQAAAAPKGLPANVRAKLES
                     EIAAAINSPDVKKQFNDIGFDVVASNGAQFGEFLAGETQRWKKVVDSGNLTAE"
     gene            1633..1971
                     /locus_tag="VFL49_11710"
     CDS             1633..1971
                     /locus_tag="VFL49_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912690.1"
                     /translation="MGRKILTKAVLATIPEMVELGGMGADDIAEKLGCNIGTLKVRCC
                     QAKISLRRPGSRRGRPRSDERTVRLASTTLALLQKRAVATGKTEAALAQELIEAIVRD
                     NLYDAVLDVD"
     gene            2277..>2492
                     /locus_tag="VFL49_11715"
     CDS             2277..>2492
                     /locus_tag="VFL49_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7912691.1"
                     /translation="MANSQAEGSTNVEPRKVAVACQGGGMHAAFALGVLKAILERVDD
                     KTEPRFKLVGLSGTSAGALCALMAWYGL"
BASE COUNT          523 a          724 c          734 g          511 t
ORIGIN      
        1 tcggtgtcgg caacgagtgc aacgcgttgt ccggtctcta ccacgtcgcg cgcaaggagg
       61 agttgccgga cgcaaaacag tccgcgctcg gctcgcgtgt cctctacctc gacaaggacg
      121 actaccgcat tcacggtttc gaaggaccga tcaaattgtc cgcaggttgt atccggctcg
      181 tcgaggatga cgtgaaatac ctttacaacc acacacccct gtcgactcgt gtggtcgtcg
      241 cgaactaatc gggctaccga gcctactccg cggtgaggtt tcctgagtcg accacctttt
      301 tccagcgctg cgtttcgccg gccaaaaatt ccccgaactg tgcgccattg cttgcaacca
      361 catcgaagcc gatgtcgttg aattgctttt tgacatcggg cgaattgatc gcggccgcta
      421 tttcggactc aagcttggca cgtacattcg ccggcaagcc cttgggcgct gcggcagcct
      481 gccaggaata gacgatgaga tcggcaatgc ctgcttcggt cgccgtcgga acatccggca
      541 gtgcggcagc gcgcttgtcg ctggtcaccg ccagggcctt cagtttgccc gccttgatgt
      601 gctgggcgac cgcaccgaga ttctgaaacg aaacgctggc atgaccgccg atcagatcgt
      661 tgatcgcggg gccgccgcct ttgtagggca cgtgcacgcc ggttgtgccg gtcttttgcc
      721 agaacagagc cgccgtcaga tgatcggacg atccgatgcc ggaagacgcg aacgtcacct
      781 ggtttggatt cttcttgaga tgcgcaatca gatccgccgc gttgttgacc ggaaaattcg
      841 gattcgtgac gagcacgttc ggcgtgcgca ccagcaacga caaaaggtcg aaatccttga
      901 gcggatcgta tttcagattc ttgtacagaa ccggattgat cgcatagacg ccgatcgatc
      961 cgatcagcag cgtgtaaccg tcgggcgtga ccgttttcac tccggcagcc ccgatgctgc
     1021 cattggcacc cgcgcgattc tcgatgacca cggattttcc gatccgctca ctgagcttgt
     1081 tgccggtgag ccgcgcggta acgtcggagg cgccacccgg cggaaagggc accacgacag
     1141 tgacatttcg ttccggatag tcggccgccg ggaccggttg ggtggaaacg ctcgcgaaca
     1201 tcagcagcgc aagcgcgcag gtcgccttaa gcttcattgt cggtaccccc acggacgatt
     1261 cgccgcatcc gcctgcggat atgacgattg tagcggttga ggccagcgcg gcgaaaggtc
     1321 cgttcgcgga cgcacgctgc ggcctttcgg gccgcgatca tcgccataat cggttgtcag
     1381 ctgtgatttt gccggtttca ccgccttttc aatctttgtc atttcggaaa atgccctgaa
     1441 aacagccccg caccacatta agaattttaa atcctcaaga caggtcgccg taatgcactc
     1501 gcgctatttt tggtgatgcc catcacagag gcatagccgg acgcgcgcta aattccacca
     1561 ggctgaaatt gaaccagaag ggccaatgac gcgactaatc attgagccgg gttggccaat
     1621 gggacagtca ctatgggacg caagattctg accaaggcag ttctcgccac cattccagag
     1681 atggttgaac tgggtggcat gggagcggac gacatagctg agaagcttgg gtgcaacatc
     1741 ggcacactta aggtgcgatg ttgccaggcg aagatcagcc tgcgacggcc aggaagccgt
     1801 cgtggcaggc ccaggagcga cgagcgcacc gtcaggctag ccagtaccac cctggctctg
     1861 ctgcaaaagc gtgctgtcgc gaccggaaaa accgaagcgg ctcttgcaca ggaattgatc
     1921 gaagcgatcg taagagacaa tctttacgac gctgtgctgg acgtcgatta acagatcttg
     1981 gtgagacacc gtcaacgcgt tacattgatt ggtcaacggt cctccttgct cacaagcgca
     2041 acgagaccga cccgctattg acctggcgaa agttcgcatt ccgccgccgt atctccgcca
     2101 ttgccccaaa gcgggacatc gctccagccg cgcctttctg agcctacgtt taaattgcta
     2161 caatgctcgt tgagcgtcgg ggtggctgcc aagtcatcga tcacaagtca tcgatcataa
     2221 tctccgcacg cggcgtccga tttcgtgagc caggcacgtg gcagaggagg acgaaaatgg
     2281 ccaattcgca ggcggaaggg tcgacgaacg tcgagccgag aaaagtggcc gtggcttgtc
     2341 agggcggtgg aatgcatgcg gctttcgcgc tcggcgtcct gaaggcaatc ctcgagcggg
     2401 tggatgacaa aaccgagccc aggttcaaac tggtgggctt gagcgggacg tcggccggcg
     2461 ccctgtgcgc attgatggcc tggtacggcc tt
//
LOCUS       DASSRL010000576         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_50287, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000576 DASSRL010000000
VERSION     DASSRL010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50287"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFL49_11720"
     CDS             complement(<1..362)
                     /locus_tag="VFL49_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912692.1"
                     /translation="MRKALVFLIAFCTLAFIAAPARAKTIKLSGTHTRDEIKTTCEKN
                     GGTFDPGAGNIAYGCIGKGGSVYCNKKGTCAGVCENCGGKAAPGKGGMGGILTSAPAA
                     TTRPLEPQRATRSPGRVPA"
     gene            complement(386..1042)
                     /locus_tag="VFL49_11725"
     CDS             complement(386..1042)
                     /locus_tag="VFL49_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018481093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7912693.1"
                     /translation="MLSLNLLKWATTMQSMTSRTGAAVALALSLLAIEPVVAFSTAAA
                     QGKLVIEPVAEAKLKQLPPGPLYWRIETFATLAAARAAAGPTSLPAEVAGKVWLFTLG
                     PKGRSSAGGSEVTEVGPIPPLAAPEYLLRINRATGPPGATTAVHSHPGSEAFYVLAGR
                     MSQRTPHGTMDVEVGQSVPGHGADTPMQVSSSGKVDLDQLVMFVVDATRPFSSPAKFE
                     "
     gene            1160..2344
                     /locus_tag="VFL49_11730"
     CDS             1160..2344
                     /locus_tag="VFL49_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7912694.1"
                     /translation="MKKVDTQKPDADRLAGDLTPAALPADLVRALEWLRCHLSEAVDL
                     EQLASVAGVRPRTLESHFKTFLGTSPLGWLRQTRFANARRALESGRADATVTRIALAN
                     GFGQLGRFAAEYRAIFGETPSATLRRSRSRLEGGDEAIDDEAFRLTWQAIPSVFAIAP
                     RECSEAIATLESVRQLAPGYGLPVALAAWCLGQRAAHGFSAIPEQDRDMALQLVAQAQ
                     TLAPNDALALTLASGALTLTHRLDDADRLLERALALDPWLPYAWIRRGWASTYVGDPG
                     AAFRELSIALRLAPVGPMRQIAFIGIGCAHFACERYERAARWVQNGTESFPGAVWADR
                     ISIAAAVHAGSKDEARRIARRLMRKDPHLTVGRARNAWPFPPAFMARLADGLATAGVP
                     RS"
     gene            complement(2450..2974)
                     /gene="sodC"
                     /locus_tag="VFL49_11735"
     CDS             complement(2450..2974)
                     /gene="sodC"
                     /locus_tag="VFL49_11735"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HET7912695.1"
                     /translation="MRVHVAMLAAAMVTVCGGSARADTVTVNAISGSGVGKVLGTIGL
                     SDASEGLVIMPDLAELPPGDHGFHVHVNPDCGPGAGPDGQPAAGLAAGGHYDPVSTGK
                     HLGPHGQGHKGDLPALRVDSGGNAMISVIAPQLKLADVKGRAIMIHAGGDNYLDQPSP
                     LGGGGARIACGVIK"
     gene            complement(3034..>4100)
                     /locus_tag="VFL49_11740"
     CDS             complement(3034..>4100)
                     /locus_tag="VFL49_11740"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HET7912696.1"
                     /translation="AMSIVSRRTFTKGLLASALVPAHSAVAQPGDAGCIAIIDTPHNA
                     AKAAAKLAAQNVEVVVRFFARKPQAGLREKVMASDGNVIDGVREPTILIRYGLSIVSL
                     YQYRNNLPEKFSKGLEDTGSAKAEAAADIKAALDQAKLVGQPEGSAIYFGVDFNVNRT
                     SVEPVLEYFRAVNRTVGNHYAIGIYGNGFVNRTLRQENLAAYSWISASRSHEETVAFY
                     NSGQWHLFQNQVDRRWFGPPGKCSNGLDVDTNIQNPRVSSIGAWGGDEVDQSRNQKIF
                     DQRRFAIRTTPVVHENGGGNGGCKLDSRVTRNNNVRVLAQSGAWSGVDVDEDGSADGQ
                     VKTADLTSDLATMPPWGPSA"
BASE COUNT          712 a         1331 c         1274 g          783 t
ORIGIN      
        1 gccggtacgc gaccggggga ccttgtcgcc cgctgcggtt ccagaggtcg cgtcgttgcg
       61 gccggtgcgc tggtgaggat gcctcccatg ccgcctttgc caggcgctgc cttgccgccg
      121 cagttctcgc agacgcccgc gcacgtgccc ttcttgttgc aatagacgga cccgccttta
      181 ccgatgcaac cgtacgcaat attcccggcg ccaggatcaa acgttccgcc gtttttctcg
      241 caggtggtct taatctcatc gcgggtgtgc gttccactga gcttgatcgt ttttgcccgc
      301 gccggtgcag cgatgaacgc aagcgtgcag aaggcgatca ggaagacgag agctttgcgc
      361 atgcgagctt tccctttacc ttcgttcatt cgaacttggc cggcgacgag aacggccgcg
      421 tggcatcgac cacgaacatc accagctgat ccagatcgac cttgccgctg ctgctgacct
      481 gcatcggcgt atccgctcca tgacccggca ccgactggcc gacctcgaca tccatcgttc
      541 cgtgcggcgt gcgctgactc atgcggccgg cgagcacata gaatgcttcc gaccccggat
      601 ggctgtgcac ggctgtcgtc gcgccgggcg ggcccgtggc gcggttgatg cgcaacaggt
      661 attccggcgc ggcaagcggc ggtatcggtc cgacctcggt cacctcgctg ccgcccgccg
      721 agcttcggcc cttcggcccc agcgtgaaca gccagacctt gccggcgacc tcggcgggaa
      781 gcgaggtcgg accggcggcc gccctcgccg ctgcaagtgt tgcgaaagtc tcgatgcgcc
      841 agtacagcgg tcctggcggc agttgcttaa gcttggcttc ggcgacgggt tcgatgacga
      901 gcttgccttg cgccgccgcc gtcgagaaag ccaccaccgg ctcgatcgcg agaagcgaca
      961 gcgcaagagc gacggcagct ccagtcctgg atgtcattga ctgcatggtc gtggcccatt
     1021 tgagcaaatt cagcgaaagc acgaatccgg cctgactttt tcggcaattg gggccggcca
     1081 ctatgcgaat tccgccggcg ctatgcagaa atcgcaggaa aagtggtagt ctgtacccgg
     1141 gggcaacgac gaatggtcga tgaagaaagt tgacacgcaa aagcccgacg ccgaccggtt
     1201 ggctggagac ctaactccgg cagcactgcc tgccgacctt gtccgcgcgc tcgaatggct
     1261 acgctgtcac ctttcggagg cggtcgacct ggagcagctc gcgtcggtcg ccggcgtccg
     1321 tccgcgcacc ctcgaaagtc acttcaaaac attcctcggc acgagccccc tcgggtggct
     1381 gcgccaaacg cgcttcgcca acgcccggcg cgcgctcgag agcggtcgtg ccgatgcaac
     1441 ggtgacacgc atcgcgctgg caaacggctt tggacagctc ggccgctttg cggccgagta
     1501 ccgtgcgatc ttcggagaga cgccctcggc aactttgcga cggagccgtt cccggctcga
     1561 aggcggcgac gaagccatcg atgatgaggc gtttcgcctc acgtggcagg ccattcccag
     1621 cgtgtttgcg atcgctcccc gcgagtgctc ggaagcgatc gccacgcttg aaagcgtgcg
     1681 acagttggcg cccggctacg gattgccggt tgccctcgcc gcgtggtgcc tgggccagcg
     1741 ggctgcgcac ggattcagcg ccatcccgga acaggatcgc gacatggcgc tccagctggt
     1801 tgcccaggcg cagaccttgg cgccgaacga cgcgctggcg ctgaccttgg cgagcggcgc
     1861 cttgacactc acgcaccggc tcgatgacgc ggaccgcctg ctcgagcgcg ccctggcgct
     1921 cgatccatgg ctaccctacg cgtggattcg ccgcggctgg gcatccactt atgtcggcga
     1981 tccgggagct gccttccgcg aactgagcat cgcattgcgg ctcgcgccgg tcgggccgat
     2041 gcggcagatt gcgtttatcg gaataggatg cgcgcatttt gcatgcgagc gctacgagcg
     2101 cgcggcgcgc tgggtgcaaa acggcacgga gtcgtttccg ggtgcggtat gggccgaccg
     2161 catatcgatc gccgctgcgg tgcatgccgg gagcaaggat gaggcacgcc gcattgcgcg
     2221 gcggcttatg cgcaaggatc cgcacctaac agtcgggcga gcgaggaatg cctggccatt
     2281 cccgccggct ttcatggcac gtcttgccga cggcctcgca accgccggtg tcccgcgaag
     2341 ctaaatcgca gagcgtgcgt gtccgcgacg ccgctaatgt tttctcagag cggtttgccg
     2401 ccgagatggc tgacttccgg tgagaaccag aagccagcct tggcaggccc tactttataa
     2461 ccccacaggc gatacgcgcg ccgccgccac ccagcggact cggctggtcc aaataattgt
     2521 ctccgcccgc atggatcatg atcgcccggc ctttgacgtc ggctaacttc agttgcggcg
     2581 ctatgactga aatcatggcg ttgccgccgg aatcgacacg cagcgccggg aggtcgcctt
     2641 tgtggccctg accgtgcggt ccgagatgtt tgccggtgct gaccggatca taatggccgc
     2701 cggctgccaa gcccgcggcc ggctggccgt ccgggccggc accgggtccg caatccggat
     2761 tgacgtgaac atgaaagcca tgatcgccgg gaggcagttc tgcaagatcg ggcatgatca
     2821 ccaggccttc gcttgcatcg gacagaccga tggtgccaag gaccttgccc acgccactgc
     2881 cactgatcgc attcacggta acggtgtctg cacgggctga gcccccgcag acggtaacca
     2941 tcgcagccgc aagcattgca acatggactc tcattagagc cccccataaa gcagtctacg
     3001 ccactaagta tctaaacacc gcggacgcac tgtttatgcc gatggtcccc agggaggcat
     3061 cgtcgcgaga tcggatgtca gatcggcggt cttgacctgt ccgtcggcgc taccgtcttc
     3121 atcgacatcc acgccggacc atgcgccgga ttgcgcaaga actctgacat tgttgtttcg
     3181 cgtcacgcgg ctgtcgagtt tgcagccccc attgcctccg ccgttttcgt gaacgaccgg
     3241 cgtggtgcgg atcgcgaagc ggcgctggtc gaaaatcttc tgattccggc tttggtcgac
     3301 ttcgtcccct ccccacgcac cgatgctgga gaccctgggg ttctggatgt tcgtatcgac
     3361 atcgaggccg ttcgagcatt tgccgggcgg cccgaaccag cggcggtcaa cctggttctg
     3421 gaagaggtgc cattggccgc tgttgtagaa agcgacggtt tcctcgtgcg accgcgacgc
     3481 gctgatccag ctgtaggccg caaggttttc ctggcggagc gtccggttga caaagccgtt
     3541 gccgtaaatc ccgatcgcat agtggttgcc gaccgtccgg ttgacggcac gaaaatactc
     3601 gagaacaggc tccacgctgg tacgattcac gttgaagtcg acgccgaaat agatcgccga
     3661 tccttccggc tgcccgacca atttggcctg gtcaagcgcg gcctttatgt cggccgcagc
     3721 ttccgctttt gccgaaccgg tgtcttccag tccctttgaa aatttctcgg gaagattatt
     3781 gcgatactgg tagagcgaaa cgatcgaaag accgtacctg atcaaaattg tcggctcgcg
     3841 cacgccgtcg atcacgttgc cgtcggacgc catgaccttc tcgcgcagac ccgcctgcgg
     3901 ctttcgtgcg aaaaaccgca cgacgacctc cacattctgg gcagccaact tcgccgcggc
     3961 ctttgccgca ttgtgcgggg tatcgataat ggcaatgcat cccgcgtcgc cgggttgcgc
     4021 caccgccgaa tgggccggca ccagggcgct ggcgagcagg cccttggtga acgttcttcg
     4081 cgatacgatc gacattgccc
//
LOCUS       DASSRL010000577         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_540418, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000577 DASSRL010000000
VERSION     DASSRL010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540418"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /gene="dnaG"
                     /locus_tag="VFL49_11745"
     CDS             complement(<1..1549)
                     /gene="dnaG"
                     /locus_tag="VFL49_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7912697.1"
                     /translation="MRFTPQFLDELKARLPVSEVVGRRVKLVRAGREFKGLSPFNKEK
                     TPSFFVNDQKQAWFDFSSGKNGSIFDFVMESEGVSFPEAVERLAQMAGMPLPKVSHEE
                     EARDARRKTLHDVMELAAKFFQDTLAARAGAKARGYLADRGLDAATQLKFRLGYASGE
                     RYALKEHLGSHGIPVEDMVETGLLISGDDIPVPFDRFRDRVMFPITDLRGRVIAFGGR
                     ALEKDAQAKYLNSPETPLFHKGGTLYNIAAARQASHDGAPIVAVEGYVDVIAMVSAGY
                     PATVAPLGTALTEDQLGLLWKMADEPILCFDGDSAGVRAAYRAVDLAMPRLRPGKSLR
                     FALLPQGQDPDDLVRSGGREAVSDVIGAARPLAEMLWARETEGHTFDTPERRAALEAR
                     VNDVTATIADEAVRRYYRQDFNARLSRFFAPAQAQQRGAGAGRWREPRAGSWRERRNG
                     DWPPRDAPRTGGKATPYVVVSQQLASSPVHRGHRTAVPRREALILQAALNHPWLLHDH
                     LEELASLE"
     gene            1759..2082
                     /locus_tag="VFL49_11750"
     CDS             1759..2082
                     /locus_tag="VFL49_11750"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET7912698.1"
                     /translation="MAERNFDALFASVRSFGWNPKKRESNLEDHKIDFEDAKVIIDDY
                     TFVRRSDRHGEIRYQIFGYLEGKEITVACTLRGTLCWIISARRASREERRKYYRRLAI
                     QSRQG"
     gene            2108..2344
                     /locus_tag="VFL49_11755"
     CDS             2108..2344
                     /locus_tag="VFL49_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HET7912699.1"
                     /translation="MTDEQIEAAIARDPDWQEMKEFDWSDVVLVTPPKKMPISIRIDQ
                     DILEYFKRGGPGYQRRMNAVLRSYVQQKKAKKRT"
     gene            2349..3056
                     /locus_tag="VFL49_11760"
     CDS             2349..3056
                     /locus_tag="VFL49_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7912700.1"
                     /translation="MTSTTFLLVHGAWGGGWHWRRVADRLRLHGHIVYAPTLTGLGER
                     LHQLHPGVDLSLHVADVLGVIKYESLNDIVLVGHSYGGFVISGVAEAIRGKIRSIVFL
                     DAFVPNDGEALVDIVQPVVQEVIRGAQARGDNMVPIRDAAAFHMNEKDRAWADSLANP
                     QPLGTFLEKIWLSGARDRIANKTYIRATGYPNVSFDTFYARAKADPSWRTYEVNCGHH
                     IMIDAPDRLTDILIEVA"
     gene            3125..>3242
                     /locus_tag="VFL49_11765"
     CDS             3125..>3242
                     /locus_tag="VFL49_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7912701.1"
                     /translation="MEARTYKTALIVGAGEGLSASLARLFASEGIRVALAARQ"
BASE COUNT          618 a         1036 c         1008 g          580 t
ORIGIN      
        1 actcgagcga ggcaagctcc tccagatggt cgtgcagcag ccacggatgg ttgagtgccg
       61 cctgcaggat gagcgcctcg cgccgcggca ccgccgtgcg gtggccgcga tgcaccgggc
      121 tcgacgccag ctgctggctc accaccacgt aaggcgtcgc tttgcccccg gtgcgcggcg
      181 catcgcgggg cggccagtcg ccattgcggc gctcgcgcca gcttccggcg cgcggttcgc
      241 gccaccgtcc ggcgcccgcg ccgcgctgtt gcgcctgcgc gggcgcgaag aaccgggaaa
      301 gacgcgcgtt gaaatcctgc cggtaatagc ggcgtaccgc ctcgtcggcg attgtcgccg
      361 tgacgtcgtt gacgcgcgcc tccagcgccg cacggcgttc cggcgtgtcg aaggtatgac
      421 cctccgtctc gcgcgcccac agcatttccg cgagcgggcg cgccgcgccg atgacgtcgc
      481 tgacggcctc gcgcccgcct gagcgcacaa gatcgtccgg gtcctgtccc tgcggcagca
      541 gcgcgaacct cagactcttg ccgggccgca ggcggggcat cgccagatcg acggcgcggt
      601 aggcggcacg tacgcccgcg ctgtcgccgt cgaagcagag gatcggctcg tcagccatct
      661 tccagagcag accgagctga tcttcagtga gcgcagtgcc gagcggtgcc acggtcgcag
      721 ggtaacctgc gctcaccatg gcgatcacgt cgacatagcc ttcgaccgcg acgatcggtg
      781 cgccatcatg tgaggcctgc cgcgcggcgg cgatattgta gagcgtgcca cctttgtgga
      841 agagcggcgt ctccggcgag ttgagatatt tcgcctgcgc atccttctcc agcgcgcggc
      901 caccgaaggc aatcacgcgc ccgcgcaagt cggtgatcgg aaacatcacc cggtcgcgga
      961 agcggtcgaa aggcaccgga atgtcgtcgc cggagatcag gagcccggtc tcgaccatgt
     1021 cctcgaccgg aatgccgtgc gagccgagat gctccttcag cgcgtagcgt tcccccgatg
     1081 catagccgag gcgaaacttg agctgcgtcg ccgcgtcgag gccgcggtcg gcgagataac
     1141 cgcgcgcctt ggcaccggca cgtgcggcaa gcgtgtcctg gaagaacttc gcggcgagtt
     1201 ccatcacgtc atgcagcgtc ttgcgccgcg catcgcgcgc ttcctcctcg tgcgagacct
     1261 ttggcagcgg catgcccgcc atctgcgcca ggcgttcgac cgcttccgga aagctcacgc
     1321 cttccgattc catcacaaag tcgaagatgc tgccgttctt gccggaggag aaatcgaacc
     1381 aggcctgctt ctggtcgttc acgaagaagg acggcgtctt ctccttgttg aacggtgaca
     1441 gacccttgaa ctcgcggcct gcgcgcacga gcttcacgcg ccggccgacg acctccgaaa
     1501 ccggaagccg ggccttgagc tcgtcgagga actggggcgt gaagcgcatg gtttttgggc
     1561 gaatcgctgc gctgatcata gtgcgatgaa tcaggcgatg ccgagaatcg gcggattcca
     1621 ttcgtttgac cgatgggttc gcagcctttt cctagttatt cacagggccg cctgccacag
     1681 cctcacagcg gccagccttg acgccaccgt catacatttg tatatacaaa aagcggccgg
     1741 cgagggtttg acgccccgat ggctgaaaga aatttcgatg ctctgtttgc gagtgttcgc
     1801 agtttcggat ggaacccgaa aaaacgcgaa agcaatctgg aagaccacaa gatcgatttc
     1861 gaagacgcga aagtaattat cgacgactac acattcgtcc gccgatcgga tcgtcatggc
     1921 gaaatcaggt accaaatctt cggatatctc gaaggcaaag aaatcacagt tgcgtgcacg
     1981 ctccgcggca cgctctgctg gatcatctcg gcgcggcgcg cgagtcgcga ggaaagacga
     2041 aaatattacc gtcgtctcgc gatacaatca cgacaagggt agaactgact ggaaagcact
     2101 cgatgccttg actgacgaac aaattgaggc tgcgatcgcc agggatccgg actggcagga
     2161 aatgaaagag ttcgactggt ccgatgtcgt tcttgtcacg ccaccgaaga agatgcccat
     2221 atcgatcaga atcgaccagg acattctcga gtattttaaa cgcgggggtc ctggctatca
     2281 gcggcgcatg aatgcggtgc tgcgttccta tgtgcagcaa aaaaaggcca agaagcggac
     2341 ctgatagcgt gacctccaca accttcctcc tcgtccacgg cgcctggggc ggcggctggc
     2401 actggcggcg cgtcgccgac cggctgcgct tgcacggcca tattgtctat gcgcccaccc
     2461 tcaccggcct cggcgagcgc ctgcaccagc tgcatccggg cgtcgacctg tctctgcacg
     2521 tcgccgacgt gctcggcgtc atcaagtacg aaagcctcaa cgacattgta ctcgtcggcc
     2581 attcctacgg cgggtttgtg atctccggcg ttgccgaggc gatccgcggc aagatccgct
     2641 ccatcgtctt tctcgacgcc ttcgttccca atgacggcga agctctggtc gatatcgtgc
     2701 agccggtggt gcaggaggtg atccgcggtg cacaggcacg cggcgacaac atggtgccga
     2761 tacgcgacgc cgcagccttc cacatgaatg agaaggaccg cgcctgggcg gattcgctcg
     2821 ccaacccgca gccgctcggc acgtttctcg agaagatctg gctcagcggc gcgcgcgacc
     2881 ggatcgcgaa caaaacctac atccgcgcca cgggctatcc gaacgtgtca ttcgacacat
     2941 tttacgcgcg ggccaaggcc gatccgtcgt ggcgcactta tgaagtgaac tgcgggcacc
     3001 acatcatgat cgacgcgccc gatcggctca ctgatattct gatcgaggtg gcctgaggcc
     3061 gcccgcaact aggcaccgga tcaatcccgt tcgccgaacc agtctggcag caagggagaa
     3121 tgtcatggag gccaggacat ataagacagc attgatcgtc ggcgccggcg agggcttgag
     3181 cgcttcgttg gcgagactct ttgccagcga gggaatccgc gtcgccttgg ccgcccggca
     3241 gg
//
LOCUS       DASSRL010000578         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_977708, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000578 DASSRL010000000
VERSION     DASSRL010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977708"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="soxC"
                     /locus_tag="VFL49_11770"
     CDS             complement(<1..1040)
                     /gene="soxC"
                     /locus_tag="VFL49_11770"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HET7912702.1"
                     /translation="MTNPIDKDGATRRRFLAGAAGLAAAGASGARAENAKNLPPNIPG
                     WTRQLGDGVGVRAYGNPSKFEKEVIRRTVPWLTATPESSVSFTPLHQLDGIITPNGLC
                     FERHHGGVAEIDPGDYRLMVHGLVDKPLIFTLDDLRRLPRINKIYFLECAANSGMEWR
                     GAQLNGCQYTHGMVHCVQYTGVSLRLLLEQAGLKPNAKWIMAEGGDAAAMNRSIPVSK
                     ALDDCMIAYRMNGEMLRPEQGYPARLVVPGWEGNVWIKWLRRLKVGDQPWFTREETAK
                     YTDLLEGGKARQFTFVMDAKSVITSPSPQAPVKQKGFNIITGLAWSGRGKVNRVDVSL
                     DGGRNWRTARIDG"
     gene            complement(1112..>2226)
                     /gene="soxB"
                     /locus_tag="VFL49_11775"
     CDS             complement(1112..>2226)
                     /gene="soxB"
                     /locus_tag="VFL49_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HET7912703.1"
                     /translation="KQLGFPFLALNIRDTEWNEPAFEAMKFFEKGGVKIAVLGQAFPY
                     TPVANPRWLMPKWSFGIREEDVRANVEKARKGGAQLVVLLSHNGFDVDRKLAARVPGI
                     DVILTGHTHDALPEAIRVGQTLLVASGSHGKFVSRLDLDVRDGAVKGFRYKLIPLFAD
                     VIRPDAEMTAAVTKARSPYAQELSRVVGHSESVLYRRGNFSGTFDDLICAALLKERDA
                     EIALSPGFRWGTSVLPGHAITVEDIHNATAITYPQAYRISMTGERLKEVLEDVGDNLF
                     NPDPYYQQGGDMVRCGGLGYAIDISKPIGSRISAMTHLKSGKPIDPKKEYAVTGWASV
                     NEGTEGPPVWDLVERYVAEEKTVRIAPNTSVKISGY"
BASE COUNT          416 a          698 c          654 g          458 t
ORIGIN      
        1 ccgtcgatac gcgcggtgcg ccagttgcgt ccgccgtcga gcgaaacgtc gacgcgattg
       61 accttgccgc ggcccgacca tgcgagtcct gtgatgatgt tgaacccttt ctgctttacc
      121 ggcgcctgcg gcgacggcga ggtgattacc gacttggcgt ccatcacgaa ggtgaattgg
      181 cgcgctttgc cgccttccag caggtcggtg tatttcgcgg tttcctcgcg cgtgaaccag
      241 ggctggtcgc cgactttcag ccggcgcagc cacttgatcc agacgttgcc ttcccatccc
      301 gggaccacga ggcgcgctgg atagccctgc tcgggccgca gcatttcgcc gttcatgcga
      361 taggcgatca tgcagtcgtc caacgccttg ctcaccggga tcgaacggtt cattgcagcg
      421 gcgtcgccgc cttccgccat gatccacttc gcattcggct tgaggccggc ctgctccagc
      481 aacagcctga gcgacacgcc ggtgtactgc acgcaatgca ccataccgtg ggtgtactgg
      541 cagccgttga gctgggcccc acgccactcc atgccggagt tggccgcgca ttcgagaaaa
      601 tagatcttgt tgatgcgcgg cagccgcctg aggtcgtcca gtgtgaaaat cagcggcttg
      661 tcgacgagtc cgtgcaccat cagccggtaa tcgcccggat cgatctccgc gaccccgcca
      721 tgatgacgtt cgaaacagag accgttgggc gtgatgatgc cgtcgagctg gtgcagcggc
      781 gtgaaactca ccgaggattc cggtgtcgcc gtcagccagg gcactgtgcg acgaatgact
      841 tctttttcga atttcgacgg attgccatag gcgcgcacac cgacgccatc gcccagctgg
      901 cgcgtccagc cgggaatgtt gggaggaagg tttttggcgt tctccgcacg tgcgccgctc
      961 gcgcccgcgg cggcaagtcc ggcagcgccc gcaagaaacc ggcggcgcgt cgcgccatct
     1021 ttgtcgatcg ggtttgtcat gcgccctctt tgcagaccgc tcacgaaggc tcttatcaca
     1081 ttctatttta tgaatgtaac agctttcgac gtcagtaccc ggaaattttc acgcttgtgt
     1141 tcggcgcgat ccgcaccgtt ttttcctccg cgacgtatcg ctcgaccaaa tcccacaccg
     1201 gtggaccttc cgtgccttcg ttgacactgg cccagccggt aacggcgtat tcttttttcg
     1261 gatcgatcgg cttgccggac ttcagatggg tcatcgcgga aatgcggctg ccgatcggtt
     1321 tcgaaatatc aattgcatag ccaagcccgc cgcagcgaac catgtcaccg ccttgttggt
     1381 agtagggatc agggttgaag agattgtcgc caacgtcttc gaggacctcc ttgagacgct
     1441 ctccggtcat cgaaatgcga taggcctgcg gataagtgat agccgttgcg ttgtgaatgt
     1501 cctcgacggt gatcgcgtga ccaggcagca cgctcgtgcc ccagcggaag cccggcgaca
     1561 gcgcgatctc agcatcacgc tccttcagga gcgcagcgca aatgaggtcg tcgaatgtgc
     1621 cgctgaaatt gccgcgccga tacagcaccg attcggaatg gccgacgacg cgtgaaagct
     1681 cctgcgcata agggctgcgc gccttggtaa cagccgccgt catctccgca tccggcctga
     1741 tgacgtcggc aaagagcggg atcaatttgt agcggaatcc cttgacggcg ccatctcgga
     1801 cgtcgagatc gagccgcgaa acaaatttcc cgtggctccc agacgccacc agcaaagtct
     1861 ggccgaccct gatcgcctcc ggcagcgcat catgcgtgtg accggtcaaa atcacgtcga
     1921 tgcccggcac gcgcgctgca agcttgcggt cgacatcgaa accgttgtgc gacaggagca
     1981 cgacgagctg agcgccccct ttgcgcgcct tttcgacatt cgcacgtaca tcctcctcgc
     2041 ggatgccgaa cgaccacttc ggcatcagcc agcggggatt ggcaacgggc gtgtatggaa
     2101 aggcctggcc gagcaccgcg atcttcacgc cacctttctc gaagaatttc atcgcttcaa
     2161 aggccggctc attccattcg gtgtcgcgga tattgagggc gagaaacgga aaaccgagct
     2221 gcttga
//
LOCUS       DASSRL010000579         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1484963, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000579 DASSRL010000000
VERSION     DASSRL010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484963"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1240
                     /locus_tag="VFL49_11780"
     CDS             77..1240
                     /locus_tag="VFL49_11780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7912704.1"
                     /translation="MSRGKSGPRQPGGGRQQAGPRHQSRQARRQSFRHPEVRPQRAVA
                     RALPGGAGAEGFPASGKVHTVSVTADESGMRVDRFFEARFPGLSFSHIQRIIRKGEVR
                     VNGKRTQPKARLDVGQSVRIPPLKIEAPAPRDDAPAAQKDRAFLKSITLYEDADVMVL
                     NKPMGLAVQGGSGTTRHIDGMLGALRGPDGQRPRLVHRLDKDTAGCLLVAKTRFAATA
                     LAKTFRSRSARKIYWALVAGVPKPRQGRISTFLAKEEREEDSFMRIAAHGEKGASHAV
                     TYYAVVETAAQRVAWLSLKPVTGRTHQLRAHMAHINHAIVGDPKYFNKENWELPGGIQ
                     SRLHLLARRIAVPHPRGGVIDVSAPLPPHMEQSWNLLGFDAKRYDPIEEAPEE"
     gene            1291..1707
                     /locus_tag="VFL49_11785"
     CDS             1291..1707
                     /locus_tag="VFL49_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912705.1"
                     /translation="MLSMLVLAVAPAATAYAQSGSGYSVLSDDYSIMVPEKGYKPSKP
                     EPWLAPKYKSPRGTVKRAVIPRSTTVPQPNAVTPPPLYVPETGRTLPNLPTISGSGRG
                     GAETFQDRAARCAHQAGVYGPNAGNRNAYVGSCVNQ"
     gene            complement(1768..2640)
                     /locus_tag="VFL49_11790"
     CDS             complement(1768..2640)
                     /locus_tag="VFL49_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET7912706.1"
                     /translation="MSVRASLSLRKNHQAKPPKRKGRNGGITEREIKDVEELATPRTP
                     VIYEVVRRLGEEEMGRPLTSLWWSGVAAGLSISFSLLSQAILQTHLPESPWQPLVVSF
                     GYCIGFIMAVLSRQQLFTESTITAVLPVAAELTGHNVGRMARLWAIVLAANLAGTLFA
                     ALFCTFTPVIPNEIYEGMLAISRDLLAFGWWDMVFRAIAAGFLMAAMVWLMPGAEGAQ
                     FHVITLMTWLIAVGGFTHIVAGSMESYLLVLSGDWAWWRMLTDFMVPVLIGNMIGGTA
                     LFALIAYAQVMDEI"
     gene            2736..3050
                     /locus_tag="VFL49_11795"
     CDS             2736..3050
                     /locus_tag="VFL49_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7912707.1"
                     /translation="MDTVFKALADASRRELLDRLRAENGQTLGELCQRLAMTRQAVSK
                     HLAILEEANLVATVKRGRAKLHYLNPVPIHEIGERWIGKFERSRLQALSDMKKALETG
                     ES"
     gene            3050..>3145
                     /locus_tag="VFL49_11800"
     CDS             3050..>3145
                     /locus_tag="VFL49_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET7912708.1"
                     /translation="MAKPRFVYVTYIRTTPEKLWAALTDPQTIRKY"
BASE COUNT          650 a          966 c          993 g          536 t
ORIGIN      
        1 cggagcgcgg cttcgagcgc gaaatcagga agcggctcga atactgggca aaactgcgca
       61 aggaacgggg caggccatga gccgcggaaa atccggcccg cgccagcccg gcggcggccg
      121 tcagcaggcc gggccacggc accaatcgcg ccaggcgcga cgccagtcat tccgtcatcc
      181 tgaggtgaga ccgcagcgcg ccgtggcgcg ggcgctgccc ggtggggctg gcgcggaagg
      241 attcccggcg tccggcaaag ttcacacggt ttccgtcacg gccgatgaat cgggcatgcg
      301 tgtcgaccgc tttttcgagg cgcgatttcc ggggctgtct ttcagtcaca tccagcgcat
      361 cattcgcaaa ggcgaggtgc gggtgaacgg caagcgcacg cagccgaagg cgcggctcga
      421 cgtcgggcaa tcggtgcgca ttccgccgct gaaaatcgag gcgccggcgc cacgcgacga
      481 tgcgccggcg gcgcaaaagg accgcgcgtt cctgaaatcg atcacgcttt acgaagatgc
      541 cgacgtcatg gtgcttaaca agcccatggg tcttgcggtg cagggtggct ccggcacgac
      601 gcgtcacatc gacgggatgc tgggcgcatt gcgtgggccc gacggccagc gtccgcgcct
      661 ggtgcaccgg ttggacaagg acactgctgg gtgtctgctc gtggcgaaga cgcggtttgc
      721 cgccacggcg ctcgcaaaga ccttccgctc gcggtccgcg cgcaagatct actgggcgct
      781 cgttgccggc gtgcccaagc cgcggcaggg ccgcatctcg accttcctcg ccaaggaaga
      841 gcgtgaggag gattccttca tgcgcattgc cgcccatggc gagaagggcg caagtcatgc
      901 ggtgacttac tacgcggtgg tcgagacggc ggcgcagcgc gtcgcctggc tttcgctcaa
      961 gccggtgacc gggcggacgc accagctgcg tgctcacatg gcgcacatta atcacgcgat
     1021 cgtcggcgac ccgaaatatt tcaacaaaga gaactgggag ttgcctggcg gtatccagag
     1081 ccgactgcat ttgctggccc gccggattgc cgttccgcac ccgcgaggcg gcgttattga
     1141 cgtttcggcg ccgctgccgc cgcacatgga gcagtcgtgg aatttgctcg gatttgacgc
     1201 gaaacgctat gatccgatcg aggaagcgcc ggaggagtag caggccgggc gaggggttcc
     1261 tatttgagag gagtcatgcg cgcaacgttt atgctgagta tgctggttct ggcggtcgcg
     1321 cccgcagcaa ctgcctatgc gcagagcggc agcggctata gcgttctcag tgacgattac
     1381 agcatcatgg tcccggagaa gggatacaag ccgtcgaagc ccgaaccctg gcttgcaccg
     1441 aaatacaagt cgccacgagg cacggtaaaa cgcgccgtga ttccgcgctc aacgacggtg
     1501 ccgcaaccca atgcggtgac cccaccgccg ctctacgtgc cggaaaccgg ccggacatta
     1561 cccaacttgc cgacgatttc aggctcaggc agaggcggtg ctgagacctt ccaggatcgc
     1621 gcggcgcgtt gcgcccatca ggccggggtc tacggaccga acgccggtaa ccgcaatgcc
     1681 tatgtcggta gctgcgtgaa tcagtaataa tcgcacggtt gagttagccg catttcttca
     1741 agttaacgtt ttgcttgagg tgcgcgatta aatctcgtcc atcacctggg cataggcaat
     1801 cagcgcgaac aaggcggtgc cgccgatcat attgccgatc aggacaggta ccatgaaatc
     1861 ggtgagcatt cgccaccagg cccaatctcc ggaaagcacc agaagatagg actccatgct
     1921 gccggcgacg atatgggtga agccgccgac cgcaatcagc catgtcatca gcgtgataac
     1981 gtggaattgc gctccctccg cgcccggcat cagccacacc atggccgcca tcaggaagcc
     2041 cgcggcaatg gcgcggaaga ccatgtccca ccagccgaag gcgagcagat cgcgactgat
     2101 agccaacatg ccttcataga tttcattcgg tattacgggc gtaaacgtgc agaacaacgc
     2161 cgcaaacagg gtgccggcga gattcgctgc gagcacgatc gcccaaagcc gcgccatgcg
     2221 cccgacattg tgcccggtca attccgctgc caccggcaat acagcggtaa ttgtgctttc
     2281 agtgaacagc tgctgccgtg aaagcacggc catgatgaat ccgatgcaat aaccgaaact
     2341 gaccacgagc ggctgccagg gcgactccgg cagatgcgtc tgcaggatcg cctgcgacag
     2401 gagcgaaaaa ctgatcgaaa gtccggcggc tacgcccgac caccacaacg acgtgagggg
     2461 gcgccccatc tcttcctcgc cgagccggcg cacgacctcg tagatgaccg gagttcgtgg
     2521 ggtggccagc tcctcgacgt ccttgatctc ccgctcggta atgccgccat tgcggccctt
     2581 ccgttttggc ggtttggcct ggtgattttt tcggagggac agggacgcgc gcacgctcat
     2641 gaccgccgtc aacgcaaggt ccgcgatgca agttccacag gcgaaacccc acgcctgtcc
     2701 ttgacatgca actaaatggt tgcatattat aatttatgga tacggtcttc aaagcgcttg
     2761 ccgatgcgag ccggagggaa ttgctcgatc gcctgcgtgc cgagaacgga caaaccctgg
     2821 gcgagctttg ccagcgcttg gcgatgacgc gacaggcggt cagcaagcac ctggccattc
     2881 tcgaggaggc caatctggtc gcgaccgtca agcggggccg cgcgaagctg cactacctca
     2941 atccggtgcc gatccacgag atcggcgaac gctggatcgg gaagttcgaa cgctcacgcc
     3001 tgcaggcgct gagcgacatg aaaaaagcac tggagacggg agagtcgtga tggccaaacc
     3061 gcgtttcgtc tacgtcacct atatccgcac cacgccggag aaactgtggg cggcgctgac
     3121 tgatccgcag acgatcagga aatac
//
LOCUS       DASSRL010000580         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_715010, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000580 DASSRL010000000
VERSION     DASSRL010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715010"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..954)
                     /gene="xth"
                     /locus_tag="VFL49_11805"
     CDS             complement(163..954)
                     /gene="xth"
                     /locus_tag="VFL49_11805"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7912709.1"
                     /translation="MRIATWNVNSIRQRMDNLQAWLEERTPDIVCLQEIKCTDDAFPR
                     EPFEALGYNVAVHGQKTFNGVALLSKLPFDEVAPGLIGDDADVQARFLEAIVSTKTGA
                     LRVVSLYLPNGNPPATDKYVYKLGWMSRLINFSRERLKLEEPMLLAGDYNVIPAAADA
                     RRPEAWTNDALFLPQTREKYRALINLGLTDALRAVSDASDLYSFWDYQAGAWEKDNGI
                     RVDHVLLSPQAADRLTGAGIDRHVRGWDKPSDHVPVYVDLDIEGR"
     gene            1128..2282
                     /locus_tag="VFL49_11810"
     CDS             1128..2282
                     /locus_tag="VFL49_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7912710.1"
                     /translation="MSPDRKLPMRNKKWLIGGAALALVGIAVLVRGLWTTDGVAARTQ
                     GEARTVPVETATAERKTMPVQLEALGTVTPMAAVAIKTQVDTTITQVLFRDGARVEKG
                     DALFTLDCRQIEADMKRVEAVINGAQATFEQAQRDVERYTELAARNATPIVTLNNAQT
                     QANISKATAESNRAQLENLKVQLGFCTIRAPISGRISMANVKIGNFVRQADTSPMATI
                     VQMAPVYVSFTVPQKNLPDIRKAIAAETATVEAVIPGEQKRASGQVTMIENTIDMATG
                     MATIRATMPNKDELLWPGTLVTADMTLRSEEGVVVPSTAVQVSQSGNFVFVVKEGVAK
                     VQNVKVERQIGRETVIASGLDGGETVVTDGQLLLSDGTRVNPRPPKVAGS"
     gene            2286..>2836
                     /locus_tag="VFL49_11815"
     CDS             2286..>2836
                     /locus_tag="VFL49_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7912711.1"
                     /translation="MNISETCIRRPVLTTLMTASLIVFGAFAYRLLPVAALPAVDFPT
                     IQITATLPGASPETMAASVASPIERQLSTISGISSMTSSSSLGTTQITVQFDLGRNID
                     GASLDVQTALATAQRRLPVEMTTPPYFRKVNPGDFPVLYISMRSDTMPLSAIDDYAET
                     VLAPQISQLPGVAQVLVYGAQKFA"
BASE COUNT          550 a          844 c          922 g          520 t
ORIGIN      
        1 cggtctatct gcggcgctgg ctggagacgc gaagggagta atttcagaaa gcaaagaagt
       61 attccgtcat ggccgggctt gtcccggcca tccacgtctt tgggctactc gtccattcaa
      121 gacgtggatg cccggcataa agccgggcat gacgaaaaag gactagcgcc cctcaatatc
      181 caggtcgacg taaacgggca cgtggtccga cggcttgtcc cagccgcgca cgtggcggtc
      241 gatgccggcg ccggtcagtc tgtcggcggc ctgcggtgac agcaggacgt ggtcgacgcg
      301 gatgccgttg tccttttccc aggcgccggc ctgataatcc cagaagctat agagatcgga
      361 tgcgtcgctg acggcgcgca gcgcgtcggt gaggccgaga ttgatcaacg cgcggtactt
      421 ctcacgcgtc tgcggcagga acagcgcgtc attcgtccag gcttcgggac ggcgcgcgtc
      481 ggctgcggcc gggatgacat tgtagtcgcc ggcgagcagc atcggttctt ccagtttaag
      541 tcgttcacgc gagaaattaa taagccggct catccagcca agcttgtaaa catatttgtc
      601 ggtggcgggc ggattgccat tgggaagata aaggctgacg acgcgcagcg cgccggtttt
      661 tgtggagacg atcgcttcca ggaagcgcgc ctgcacgtcg gcgtcatcgc cgatcagtcc
      721 gggcgcgact tcgtcgaagg gaagcttcga caaaagtgca acgccgttga aggtcttctg
      781 cccatgcacg gccacgttgt acccgagcgc ctcgaagggc tcgcgtggga aggcatcatc
      841 ggtgcacttg atctcctgca ggcagacaat gtcgggtgtc cgctcctcga gccaggcttg
      901 caggttgtcc atgcgctggc ggatggaatt gacgttccag gtggcgattc tcatcgattt
      961 tggcgttcgg ggaggggcga ataatgctgt attcaccgtg tttatcaggc agcgcaggga
     1021 tatgcacgat ttaacgttta tatgctagga aagccgccaa cgcggcttcc ggtggtgcga
     1081 gaaactagca gatccgcgga agagggagag tgtccgggcg gggcccgatg agtccggacc
     1141 gaaagttacc gatgcgcaac aagaagtggc ttattggagg ggcggcgctg gccctggttg
     1201 gcatcgccgt gctcgtccgc gggctctgga ccaccgacgg cgttgcggcg cgcacgcagg
     1261 gcgaggcgcg gacggtgccg gtggagaccg ccacggccga gcgcaagacg atgccggtgc
     1321 agctcgaggc cctcggcacc gtcaccccca tggccgccgt tgccatcaag acgcaggtcg
     1381 acaccacgat cacgcaagtt ctctttcgtg acggcgcgcg cgtcgaaaaa ggtgatgcgc
     1441 tgttcacgct cgattgccgg cagatcgaag ccgacatgaa gcgcgtcgag gcggtgatca
     1501 acggcgcgca ggcgaccttc gagcaggcgc agcgcgacgt cgagcgctac accgagttgg
     1561 cggcaagaaa cgcgactccg atcgtcacgc ttaacaacgc gcagacgcag gccaatattt
     1621 cgaaagcgac ggcggaatcg aaccgcgcgc aactcgagaa tttgaaagtg cagctcggct
     1681 tctgcaccat ccgtgcgccg atctccggcc gcatcagcat ggccaacgtg aagatcggaa
     1741 atttcgtgcg gcaggccgac acctcgccga tggcgacgat cgtgcagatg gcgccggtct
     1801 atgtcagctt cacggtgccg cagaaaaatc ttcccgacat ccgcaaggcg atcgcggcgg
     1861 aaaccgcgac cgtggaagcc gtcattccgg gcgagcagaa gcgcgccagc ggccaggtca
     1921 ccatgatcga gaacaccatc gacatggcga ccggcatggc cacgatccgc gcgacgatgc
     1981 cgaacaaaga cgagctgctg tggccgggaa cgctggtcac ggccgacatg acgctacgct
     2041 ccgaggaagg ggtcgttgtc ccttcgaccg cggtgcaggt cagccagtcg ggcaattttg
     2101 tcttcgtcgt caaggaaggc gttgccaagg ttcagaatgt caaggtcgag cgccagatcg
     2161 gccgcgagac tgtcattgcg tcgggcctcg atggcggtga aacggtggtg acggacggtc
     2221 agttgctttt atccgatggc acgcgcgtca atccgcgccc gccaaaagtc gccggcagct
     2281 gacccatgaa catttctgaa acctgtatcc gccggccggt gctgacgacg ctgatgacgg
     2341 cgtcgctgat cgtctttggc gcctttgcct acaggttgct gccggttgcg gcattgccgg
     2401 cggtcgattt tcccaccatc cagatcacgg caacgctgcc gggcgcgagc cccgagacga
     2461 tggcagcctc ggttgcttca ccgatcgagc ggcagctctc gacaatttcc ggcatttcgt
     2521 cgatgacgtc gtcgtcgtcg ctcggcacga cgcagatcac cgttcagttc gatctcggac
     2581 gcaacatcga cggcgcatcg ctcgacgtgc agaccgcgct tgcgacagcg cagcggcggc
     2641 tgccggttga aatgaccacg ccgccttact tccgtaaggt caatccgggt gatttcccgg
     2701 tgctctacat cagtatgcgc tcggacacga tgccgctgtc ggcgattgac gactacgccg
     2761 aaacggtgct ggcgccgcaa atctcgcagc tgcccggcgt cgcgcaggtg ctggtttacg
     2821 gcgcgcagaa attcgc
//
LOCUS       DASSRL010000581         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1013040, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000581 DASSRL010000000
VERSION     DASSRL010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013040"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFL49_11820"
     CDS             <1..600
                     /locus_tag="VFL49_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912712.1"
                     /translation="GDQMAQVLVTLGLSFMAADFCLMVWGGDPLSVNTPATLTGFARF
                     GVLIFPVYRLAIIVIAVIVAIGLWLLLERTRLGAMIRAGVDDPDMARVVGIRVSQLFT
                     IVFALGAGLAAFAGIIGGPILSVYPGLDQDMLPLALVVVILGGTGSLLGSFVGSIIVG
                     FIYNFGQALLPELAYFVLFLPMLLVLLLRPQGLFGRHVP"
     gene            597..1580
                     /locus_tag="VFL49_11825"
     CDS             597..1580
                     /locus_tag="VFL49_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912713.1"
                     /translation="MSQRAFALLVGFVALVTLPYWMTGVYYVNVASQILFYAIFALGL
                     NVLAGYGGLVSLGHAGLFGVTAYATGYMLQLGLGHSLAIVIGLVIGVAAMAIYAALSL
                     RSTGIGFIMITLALGEILWGLAYRWISLTGGDNGLSLKTRPEPFGLSLADANTFYYAT
                     LIVFLLSLGAMAIFVRSPLGAALMGTRDQPRRMNALGYHVWAIRFYACLFSGLLTAVS
                     AILFVYYTQFISPQTLALTSSAEVLLMVISGGAGTLLGPIVGSALVVVVKTVVSGFIE
                     RWNFLLGTIFVAIVILMPEGLVPGSVRLWRMAWRKRERRPAPVPGGAEAKP"
     gene            1577..2314
                     /locus_tag="VFL49_11830"
     CDS             1577..2314
                     /locus_tag="VFL49_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912714.1"
                     /translation="MSALSVKSLCKAFGGLRVTTNVDLNVEAGERRLIIGPNGAGKTT
                     LFNLITGELTPDSGSIELLGQDITHVPSRRRAHLGMARTYQIITLFARDTILHNVTLA
                     LLGLSPARWNPLIDLKRQGHFVEHAQAALEKVGLGHIANRPLAQTSYGERRRVEIAMA
                     LTQNPKVLLLDEPFAGLSIDERRDVHQLLLAIPRDVTIVMIEHNMDVALEFAERITLL
                     HFGEVIVEGNRAEVVADPRTREVYLGH"
     gene            2304..>2577
                     /locus_tag="VFL49_11835"
     CDS             2304..>2577
                     /locus_tag="VFL49_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7912715.1"
                     /translation="MATSALKLSHIDAFYGDSHVLGDVSFELGEARMLGLLGRNGAGK
                     STCMNVTMGLLPPRHGEVVVFGKPVSGMSPEMIAARGVALVPQGRRI"
BASE COUNT          391 a          829 c          789 g          568 t
ORIGIN      
        1 ggcgatcaga tggcgcaggt gctggtcacc ctcggtcttt ccttcatggc cgctgacttc
       61 tgcctgatgg tttggggcgg cgacccgctt tccgtgaata cgcccgccac cctcaccggc
      121 tttgcgcggt tcggcgtgct catttttccg gtctaccggc tggccatcat cgtgatcgca
      181 gtcatcgtgg ccatcggcct gtggctgctg cttgaacgca cgcggcttgg cgcgatgatc
      241 cgcgccggcg tcgacgatcc ggacatggcg cgcgtcgtcg gcatccgcgt ctcccaactg
      301 ttcaccatcg tcttcgccct cggtgccggt cttgccgcat ttgccggaat catcggtgga
      361 ccgatcctgt cggtctaccc gggtctcgat caggacatgc tgccactggc gctggtggtg
      421 gtgattctcg gcgggaccgg cagcctgctc ggctcctttg tcggcagcat catcgtcgga
      481 ttcatctata acttcggaca ggcgcttttg cccgaactcg cctacttcgt cctgtttctt
      541 ccgatgttgc tggtcctgct gctgcggccg cagggtcttt tcggcaggca tgtgccatga
      601 gccagcgcgc ctttgccctt cttgtcgggt tcgtggcgct cgtgacgctg ccctactgga
      661 tgacgggcgt ctattacgtc aacgtcgcaa gccagatcct gttctacgca atcttcgcgc
      721 tcgggctgaa cgttttggcc ggttacggcg gactcgtgtc gcttggtcac gctggattgt
      781 tcggcgtcac ggcctacgcc accggttaca tgctgcaact ggggctcggt cactcgctcg
      841 caatagtcat cgggctggtg atcggagtcg cggcgatggc aatctatgcc gcgctgtcgc
      901 tgcgttcgac cggcatcggc ttcatcatga tcacgctcgc gctgggcgaa atcctgtggg
      961 ggctcgccta tcgctggatc agcctcaccg gcggcgacaa cggcctgagc ctgaagacgc
     1021 gccctgagcc gttcgggctt tcgctggcgg acgccaatac gttctactat gcgaccctga
     1081 tcgtcttcct gttgtcgctc ggcgccatgg ccatcttcgt gcgttcaccg ctcggcgccg
     1141 cattgatggg cacccgcgac cagccgcgcc gcatgaatgc gcttggctac catgtgtggg
     1201 ccatccggtt ctatgcgtgc ctgttttccg gtttgctcac cgccgtgtct gccattctgt
     1261 tcgtctacta cacgcaattc atcagcccac agacgctggc gctgacctcg tccgccgagg
     1321 ttctgctgat ggtgatttcc ggcggcgccg gcacattgct tgggccgatt gtcggatcgg
     1381 cgctggtcgt tgtcgtgaaa actgtcgtga gcggattcat agaacgctgg aatttcctgc
     1441 tcggtacaat ctttgtcgcc atcgtgatac tgatgcccga aggcctggtg cccggcagtg
     1501 tgcgtctctg gcgcatggcc tggcgcaagc gtgagcggcg gcccgctccg gttcctggcg
     1561 gtgcagaggc caaaccatga gcgccctctc cgtcaaaagc ctttgcaagg ccttcggcgg
     1621 cctgcgtgtc acaaccaacg tcgacttgaa tgtcgaggca ggtgaacgcc ggctgattat
     1681 cggtccgaac ggcgccggca aaacgacgct gttcaacctg atcaccggcg aattgacacc
     1741 cgactccggg tcgatcgaat tgctcgggca agacatcaca catgtgccga gccgacgccg
     1801 cgcccatctc ggcatggcgc gcacctatca gatcatcacg ctcttcgcac gcgacaccat
     1861 ccttcataat gtgaccctgg cgctgcttgg tctgtcgccg gctcgctgga atccgctgat
     1921 cgacctcaag cgtcagggcc atttcgtcga gcacgcgcag gcggcactcg aaaaagtcgg
     1981 gctcgggcac atcgcaaacc gtccgctggc gcagacgtct tatggtgagc gccgccgcgt
     2041 cgagattgcc atggcactga cgcagaaccc gaaggtcctg ctgctcgacg agccctttgc
     2101 cggtctttcg atcgacgaac ggcgtgacgt gcatcaattg ttgctggcga tcccgcgcga
     2161 cgtcaccatc gtcatgatcg agcacaacat ggacgtggcg ctggaattcg ccgagcgcat
     2221 cacgctgctg cacttcggtg aagtgatcgt cgaaggcaac cgcgccgagg tggtggccga
     2281 tccacgcacg cgggaggtct atcttggcca ctagcgcgct caaactcagc cacatcgacg
     2341 ccttctacgg cgacagccac gttctcggcg acgtatcgtt cgagttgggc gaggcacgca
     2401 tgctcggcct gctcgggcgc aacggcgccg gcaagtcgac ctgcatgaac gtcacaatgg
     2461 gattgctgcc gccgcggcac ggcgaggtgg tcgtgttcgg caagccggtg agcggtatgt
     2521 ctccggagat gattgccgca cgcggcgtcg cgctggtgcc gcagggccgc cgcatct
//
LOCUS       DASSRL010000582         6924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1257799, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000582 DASSRL010000000
VERSION     DASSRL010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257799"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFL49_11840"
     CDS             complement(<1..477)
                     /locus_tag="VFL49_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET7912716.1"
                     /translation="MIERILTVGGLTLLSRVTGFARDIILAAVLGAGPVADAFFVALR
                     LPNHFRAIFAEGAFNAAFVTAYARIRESGGAGRAALFADRIFTLLLASQIVLLAVALL
                     FTPAVIEVLAPGFAHDPGRFALAVELTRITFPYLLLVTLVTLYSGILNALQRFAAAA"
     gene            620..2029
                     /locus_tag="VFL49_11845"
     CDS             620..2029
                     /locus_tag="VFL49_11845"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HET7912717.1"
                     /translation="MPSPIIPILLAGGAGTRLWPVSRDALPKQFLPLIGNKSTYQETL
                     LRVQDEMFAPPIVITGPSFHFFARRQAEEVGVEATIVIEPMRRDSGPAIAAATVVASR
                     RDPKAVVLALAADHIIPDLDAFRATCRAGRVAAEAGRIVTFGIKPTEPKTSYGYILPG
                     EAIGDGVHAVKSFVEKPDSKTAARYVREGYLWNSGNFLFRAAVLLSELKRLEPDMAAA
                     VDAAVANASSDLGFLRLDSEAFSRAPQKSIDYAVMERTDLAAVARGDFRWSDIGSWDA
                     LLDITSRDSAGNVLQGSVVTMDAKDCVVHSNDRLTAVVGVKDLVVVSTSDAVMVVPRA
                     RAQDVRELVGKLKTAKRPEATDHKRVHRPWGYYESIDAGERFQVKRIVVIPGGVLSLQ
                     RHRHRAEHWVVVRGTAEVTVGEKVRAVHENESIYIPIGSVHRLANKGKIPLELIEVQT
                     GSYFGEDDIERLDDIYKRT"
     gene            complement(2228..3376)
                     /locus_tag="VFL49_11850"
     CDS             complement(2228..3376)
                     /locus_tag="VFL49_11850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7912718.1"
                     /translation="MNLRTNPPPIPFIDVAAQRRRLGPAIDAAVARVLNHCQFILGPE
                     VRTFEAELAVFCGARHVVTCASGTDALVLALRAMGVGPGDAVLCPSFTFCATAEVAVL
                     VGATPVFVDVDPATFNIDARGISGAAATAREAGLSPKAIIPVDLFGLPAEHGAIQAAA
                     RAENLLVLDDAAQGFGASCDNRRLGTFGLATATSFFPAKPLGCYGDGGAVMTDDDAFA
                     DALRSLRVHGQGSDKYDNVRIGLASRLDTIQAAILIEKLKIFPDEVDARNRIANRYGE
                     GLDDVVLVPRIPAGYTSVWAQYTIRVTGGRRDALAAALKAEGIPTAIYYPIPLHRQQA
                     YKHYPVGKDGVAVSDRLAAEAISLPMHAYLDAATQDRIVDATRRALKS"
     gene            complement(3403..4407)
                     /locus_tag="VFL49_11855"
     CDS             complement(3403..4407)
                     /locus_tag="VFL49_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7912719.1"
                     /translation="MMQAAVASADRPLRVGVVGVGVMDSNHARVFAGLPGTELVGVAD
                     PDRKQADFVARTLGCVAVADVNELLERNVDAITIAAPTHLHRDIALTCIARGVHVMVE
                     KPIASSVEEGREIINVARRAGVTLMVGHVERFNPAVEAIKEAIQGEDILSIAITRVGP
                     FPPRMSNVGVVIDLAVHDIDLIRWFTDSDIVEVQPQLSSAVAEREDIALLQFRTASGV
                     LAHINTNWLTPFKARNVTVATRGKYVMGDLLTRQVTECFGFQPDGSYSMRHLSVGHAE
                     PLRSELLAFLRAVRSGVTPAVTGEEAVASLEIATRCLATRPTVVSPAAQRKGPRAVVG
                     "
     gene            complement(4573..5325)
                     /locus_tag="VFL49_11860"
     CDS             complement(4573..5325)
                     /locus_tag="VFL49_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HET7912720.1"
                     /translation="MTTPTLAKLKVKLFTDGADKAQIVEMAKQPWIAGFTTNPSLLKK
                     AGVSVYAAYARDLVAAVPDRHISFEVFSDDIAEMVAQARVITTWGKNVYVKLPVTNTR
                     DEGLYDAVRTLSREGVNINMTAIFTGEQVVRAVEALDGGAPACVSVFAGRLADFGIDY
                     RPIVQDAIARARKTRNVEIIWASTREVFNVVEADAMGCHIITAPADVLRKLPAIGSKS
                     AAELSLAAVKSFRDDAVAAGLRLAVPASRAAE"
     gene            complement(5393..6355)
                     /locus_tag="VFL49_11865"
     CDS             complement(5393..6355)
                     /locus_tag="VFL49_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HET7912721.1"
                     /translation="MVPPLLFELRGKRVYVAGHTGLAGSAIARRLASEGCEILTADRR
                     ELDLTHQDATETWIARTRPDAIFLAAARVGGIYANDAFPADFLADNLAIAVNVLRAAL
                     AAKVKKLMFLGSSCVFPRNAPQPMTEDLLLTGALEPTNEWYAVAKIAGLKLAEAYRRQ
                     FRADFISVMPTNLYGPGDNYHPENSHVPAALIRRFHEAKVKCADEVVVWGTGKPRREF
                     LAADDLGDACIFVMKHYSGDGFLNIGTGKEVSIEEFARLVADIVGYRGKLVFDANRPD
                     GPPRKLLDISKISALGWMAKTPLREGLQATYNDFLARGADVRER"
     gene            complement(6360..>6924)
                     /locus_tag="VFL49_11870"
     CDS             complement(6360..>6924)
                     /locus_tag="VFL49_11870"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7912722.1"
                     /translation="NYREAYGIHASNGILFNHESPIRGETFVTRKITRAVAAIEAGLQ
                     DQLFIGNLNARRDWGHARDYVEGMWMMLQQPKADDYVLATGASHTVREFVEHAFALIG
                     RRLRWKGEGADETGLDEASGKSLVKVDARYFRPTEVDVLIGDASKARARLGWKHRVSF
                     DALVKEMVEEDRRELRGENRARRGMNE"
BASE COUNT         1348 a         2209 c         2109 g         1258 t
ORIGIN      
        1 ggctgcggca gcgaaccgtt gcagcgcatt gaggatgcca ctgtagagcg tcaccagcgt
       61 caccagcagc agatagggaa atgtgatgcg cgtcagctct accgccagcg cgaagcggcc
      121 ggggtcgtga gcgaaaccgg gcgccagcac ctcgattacc gccggcgtga acagcagcgc
      181 caccgccagc aacacaatct ggctcgcaag caacagagtg aagatgcggt cggcgaacaa
      241 ggcggcgcgt cctgcgcctc cgctttctct tatgcgcgca taggcggtaa cgaacgcggc
      301 attgaacgcg ccctcggcaa atatggcgcg gaagtggttc ggcaagcgca gggcgacgaa
      361 gaacgcgtca gccacgggcc cggcaccgag aacggcagcc aggatgatgt cgcgcgcaaa
      421 gccggtgacg cgcgaaagca gcgtcaggcc gccgacggtc agaatgcgct caatcatcag
      481 ggaacgtccc tgccaacggg cttaaaacgc cgccccatta accacttgag gcttaggcaa
      541 cgatagcgat agtcaggtac cggcaactgc cttaagacaa ttctgcgccc gaacaaagag
      601 ccgcgacaac cgcagaccca tgccttcacc gatcatcccc attctgctgg cgggcggcgc
      661 cggcacgcgg ctctggcccg tgtcccgcga cgcccttccc aagcagttcc tgcctttgat
      721 cggcaataag tcgacctacc aggaaacgct gttgcgggtg caggacgaga tgttcgcgcc
      781 gcctatcgtc attaccggcc ccagctttca cttcttcgcg cgacggcagg cagaggaggt
      841 cggggtcgag gcgacgatcg tcatcgaacc gatgcggcgc gattcggggc ctgcgatcgc
      901 cgcggcgacc gtcgtcgcca gccgccgcga ccccaaggcc gtggtgctgg cgcttgccgc
      961 cgaccacatc attccggacc tcgacgcgtt ccgggccacc tgccgcgccg gacgcgtcgc
     1021 cgccgaggca ggccgcattg tcactttcgg catcaagccg acggaaccaa aaacaagcta
     1081 tggctacatt cttccgggcg aggcgatcgg cgatggcgtc catgcggtga aaagcttcgt
     1141 cgagaagccc gattccaaaa ccgctgcccg ctatgtccgc gagggctatc tgtggaattc
     1201 cggcaacttt cttttccgtg cggcggtgct gctgtccgag ctcaaacggc tggaacccga
     1261 catggctgcc gccgtcgacg cagcggtcgc gaatgcaagc agcgatctcg gatttctgcg
     1321 gcttgactcc gaagcattct cacgcgcgcc gcagaagtcg atcgattacg cggtcatgga
     1381 aaggaccgat ctggcagcgg tcgcgcgtgg cgatttccgc tggtcagaca tcggcagctg
     1441 ggacgcgctg ctcgacatca catcccgcga ctccgcgggc aatgtcctgc agggctcggt
     1501 cgtcacgatg gatgcaaagg attgcgtcgt gcattccaat gatcgcctga cagctgtcgt
     1561 cggcgtcaag gatctcgtgg tggtcagcac ctccgacgcc gtgatggtcg tcccccgcgc
     1621 gcgtgcgcag gatgttcgtg aactggtcgg caagctcaag acagcaaaaa ggccggaggc
     1681 aaccgatcac aagcgggtcc accggccttg gggctattac gaatcgatcg atgctggcga
     1741 gcgctttcag gtcaagcgca tcgtcgtcat tcccggcggc gtcctttcat tgcaaagaca
     1801 ccgtcatcgc gccgagcact gggtcgtggt gcgaggcact gccgaggtga cagttggcga
     1861 aaaagtccgt gccgtgcacg aaaacgagtc gatctatatc ccgatcggca gcgtgcatcg
     1921 cctggcgaac aagggaaaaa tcccgctcga attgatcgag gtgcagaccg gcagttactt
     1981 cggcgaagac gatatcgagc ggctcgacga tatctacaag cggacgtaat ctggaatcgc
     2041 cctcggattg aatcgtcatg gccgggcata gccgttcgaa gaacggcgtg ctttcgcacg
     2101 cctatgtccc ggccatccac gtctttgaat agtctttaag acgtggatgc ccgcgactag
     2161 cgcgggcatg acggtggggg ctaccaacgt caaacatctt tgcttcagac cgttattctt
     2221 cccctgctca tgacttcagc gctcgccgtg tcgcgtcgac aatgcggtcc tgggtcgccg
     2281 catcgagata ggcgtgcatc ggcaggctga tcgcctcggc ggcaagacgg tcgctgaccg
     2341 cgacgccgtc ctttccgacc gggtagtgct tgtaagcctg ctgccgatgc agcgggatcg
     2401 ggtagtagat cgctgttgga atgccttcgg ctttcagcgc agcagcgagc gcgtcacggc
     2461 ggccgcccgt gacccggatc gtgtattgtg cccacacgga ggtgtagccg gccggaatgc
     2521 gtggcaccag gacaacgtcg tcgagacctt cgccgtagcg attggcaatc ctgttgcggg
     2581 cgtcgacttc gtcggggaaa attttcaact tctcgatcag aatcgccgcc tggatcgtgt
     2641 cgagccgcga tgccaggccg atccggacat tgtcgtattt gtcgctcccc tgcccgtgca
     2701 cgcgcaggct tcgcagggcg tcggcaaacg catcatcgtc ggtcatcacg gcgccgccgt
     2761 ctccgtagca accgagcggc ttggcgggga agaaactggt cgccgtcgcc agaccgaaag
     2821 tgcctaaacg tcggttgtcg cagctcgcac cgaagccttg ggcagcgtcg tcgagaacca
     2881 gcaggttttc ggcccttgcc gcagcctgaa tagcgccgtg ctcggccggc aggccgaaca
     2941 gatcgaccgg aatgatggct ttcggactga gcccggcctc gcgcgccgtg gctgccgcgc
     3001 ccgagattcc cctggcatcg atgttgaaag tggccggatc gacgtcgaca aagaccggcg
     3061 tcgcgccaac aagaactgca acttccgccg ttgcgcagaa ggtgaatgac gggcagagca
     3121 ccgcatcgcc cggcccgact cccatcgctc gcagcgccag cacaagcgca tcggtgccgc
     3181 tcgcgcacgt cacgacatgg cgggcgccac aaaacaccgc cagttcagcc tcgaatgtgc
     3241 gcacttccgg ccccagaatg aactggcagt ggttcaggac cctggcgacc gcggcatcaa
     3301 tagcgggacc cagcctgcgg cgttgtgcgg ccacatcaat gaatgggata ggcggcggat
     3361 tggtgcgcaa attcattgaa cgtccgtatg aaaggcggcc cgtcagccaa cgacggcgcg
     3421 cggacctttt cgctgcgcgg caggagacac caccgtcgga cgcgtcgcca ggcagcgcgt
     3481 tgcgatctca agactcgcca cggcctcctc cccggtgacg gcgggcgtca cgcccgagcg
     3541 aaccgcacga aggaaggcaa gcaactccga acgcaacggc tcggcgtggc cgaccgaaag
     3601 gtgacgcatc gaatagctgc cgtcaggctg gaagccgaag cattccgtca cctggcgtgt
     3661 cagcagatcg cccatcacat acttgccgcg cgtcgccacc gtgacgttgc gcgccttgaa
     3721 gggcgtcagc cagttcgtgt tgatgtgcgc gagtacgccg gaggcggtac gaaactgcag
     3781 aagcgcgatg tcctcacgct cggcaacggc gctggaaagc tgcggctgca cttcgacgat
     3841 gtcggaatcg gtaaaccagc ggatcaggtc gatatcgtga actgcaagat cgatcaccac
     3901 gccgacattc gacatgcgcg gcggaaacgg accgacgcga gtaatggcga tcgacaggat
     3961 gtcctcgccc tggatcgcct ccttgatggc ctcgactgcc ggattgaagc gctcgacgtg
     4021 gccgaccatc agcgtgacgc cggcacggcg cgctacgttg atgatctcac gaccctcttc
     4081 gaccgacgac gcgatcggtt tttccaccat gacgtggacg ccccgcgcaa tgcaggtcaa
     4141 cgcgatatcc cgatgaagat gggtcggcgc ggcgatcgtg atcgcatcga cgttacgctc
     4201 aagcagttca ttgacgtccg caacggcgac acatccgagc gtgcgtgcga caaagtcggc
     4261 ctgcttgcgg tcgggatcag caacgccgac caactcggtc ccaggcaatc ccgcgaatac
     4321 acgcgcgtgg ttgctgtcca tgacgccgac tccgacgaca ccgacacgca acggacggtc
     4381 cgcgctagcg actgcagcct gcatcatgtc caggtggtat ccccgcgccc ctttatgtcc
     4441 gtcgcccatc atcccctacc acggcgatgg gaggctggcg acagcccctt ggaatcctta
     4501 gcgaacacgt tttgattcaa agcgttacag gtggataaga ttttacgccg ctgttacgcg
     4561 agaaatggcc gcttattccg ccgcgcgcga ggccggaacc gcgagccgca ggcctgcggc
     4621 gacggcatca tcgcggaagg atttcacggc cgcgagcgaa agttcggcag ccgacttgct
     4681 gccgatggcg gggagtttcc tgagaacgtc ggccggcgct gtgatgatgt ggcaacccat
     4741 ggcatctgcc tcaacgacgt tgaagacctc gcgcgtcgag gcccagatga tctcgacatt
     4801 gcgggtcttg cgcgcacgcg caatcgcgtc ctgcacgatc ggccgataat cgatgccgaa
     4861 gtcggcgagc cggccggcaa atacggagac gcaggcaggc gcgccgccat ccagcgcctc
     4921 gacggcacgc acaacctgct cgccggtaaa gatcgcggtc atattgatgt tgacaccttc
     4981 gcgcgaaagc gtacgcaccg cgtcgtacag cccctcgtcg cgcgtgttcg tcaccggcag
     5041 cttgacgtag acgttcttgc cccaggtggt gatgacacgc gcctgagcga ccatttcggc
     5101 gatatcgtcg gaaaagacct cgaacgaaat atgccggtca ggcacggccg caaccagatc
     5161 gcgcgcatag gctgcgtaaa cgcttacacc tgccttcttc agcaatgacg gatttgtcgt
     5221 gaagccggcg atccatggtt gcttggccat ctcgacgatc tgcgccttgt cggcgccgtc
     5281 ggtgaacagc ttgactttca atttcgccag cgttggagtc gtcatttgtt ccgatcctgt
     5341 gtggtcatgc cactataaag cgattgatcg ccgcgatcca atcgcgcgcg ctttaccgtt
     5401 cgcgcacgtc ggcgccccgg gccaggaaat cattataagt agcctgcagg ccttctcgca
     5461 ggggcgtctt tgccatccag cccagcgcgc tgattttcga aatgtccagc agcttgcgcg
     5521 gcggaccgtc gggacgattc gcgtcgaaaa caagtttccc ccgatagccg acgatatccg
     5581 caacgagccg ggcaaattcc tcaatgctga cttccttgcc ggttcctatg ttgagaaaac
     5641 cgtcacccga gtaatgcttc attacaaaga tgcaggcgtc gccgaggtca tcggctgcga
     5701 ggaactcgcg cctcggcttg ccggtgcccc agacaacgac ttcatctgca catttgacct
     5761 ttgcttcgtg gaagcggcga atgagtgcgg ccggaacgtg gctgttctcg gggtgataat
     5821 tatcgcccgg gccgtagaga ttcgtcggca tgaccgaaat gaagtcggcg cggaactgcc
     5881 gccggtaggc ctccgccagc ttgaggccgg cgatcttggc cactgcatac cattcgttgg
     5941 tcggctcaag cgcgccggtc aacagcaggt cttcggtcat cggctgcggc gcattgcgtg
     6001 gaaacacgca ggatgagccg aggaacatca gcttttttac tttcgcggcg agagccgcgc
     6061 gcagcacatt gacagcgatg gcgagattgt cggccagaaa gtcagcggga aaggcatcgt
     6121 tggcgtagat gccgccgacc cgcgctgcgg caaggaaaat tgcatccggc ctggttcgcg
     6181 caatccatgt ctcggtcgcg tcctgatgcg tcagatcaag ctcgcgccgg tccgccgtca
     6241 ggatctcgca tccttccgac gcaaggcgcc gcgcaatggc ggaccctgcc aggccggtgt
     6301 ggccagccac gtagacgcgc ttgcctcgca attcgaacag caaaggcggc accatcgaat
     6361 tactcgttca tgccgcgtcg ggctctgttt tcgccgcgca attccctgcg atcctcttcc
     6421 accatctctt tcaccagcgc atcgaagctc acgcggtgct tccagccaag cctggcgcgc
     6481 gccttcgagg catcgccgat caggacatcc acctccgtcg gccggaaata ccgcgcgtcg
     6541 accttgacca gcgacttgcc gcttgcctca tcgaggccgg tctcgtcagc tccctcgcct
     6601 ttccacctga gccggcggcc gatcagcgcg aaggcgtgct cgacaaattc gcgcaccgta
     6661 tggctcgcgc ccgttgccaa cacataatcg tcagccttcg gctgctgcag catcatccac
     6721 atgccttcga cgtagtcccg cgcatgcccc cagtcgcgtc gggcattgag gttcccgatg
     6781 aacagctggt cctgcaagcc cgcctcgatc gccgcgaccg cacgggtgat cttgcgcgtg
     6841 acgaaggttt cgccacggat cggactctcg tgattgaaca agattccgtt ggaggcgtgg
     6901 atcccataag cctcgcgata gttc
//
LOCUS       DASSRL010000583         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_679880, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000583 DASSRL010000000
VERSION     DASSRL010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679880"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..926
                     /locus_tag="VFL49_11875"
     CDS             15..926
                     /locus_tag="VFL49_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912723.1"
                     /translation="MRFAVTNPPHFLPIWLGRDAGIFAKHGLDVEIIFMRGGALITMG
                     ILSGELQLSGVGAESVVAAKLEGGDAVLLACPLDTDLVYLIGRPDIKTPEQLKGKSTA
                     VTRLGSSIHFYLRAALKFLGMDADKDMTILQLGGGPEIAAALETGRISAGILTIRYAL
                     PFLQRGWPVLIDLSKTDLKYPPSCVGSGRSFVRKNLNTIERFLKAYTEAIHVIKTDGA
                     LAKRVYAKEYRESDAAVVDKIVTVYAGLFKSVPTVPASGLETVIREAAARKPLPSDAF
                     RQPDFYKDDAPLQKIIKDGWIEQLRKR"
     gene            996..1553
                     /locus_tag="VFL49_11880"
     CDS             996..1553
                     /locus_tag="VFL49_11880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET7912724.1"
                     /translation="MLLIVDMENEDAHPAGKRYMGEAVEEIIPNIAALRRRVREAGGL
                     VVHTQSVRKPDALEFTLFKNVVRKVEGSWGAEFIDQLKPASGEPVVVKYTHDCFYRTE
                     MESLLERLRLRAGEGRIIVTGIAARGCVQCAVMGFSIRDFYVYVPIDCTTQRDQKEKL
                     QAFSQFASFGYRYNVTVTRSDLIQF"
     gene            complement(1675..>3250)
                     /locus_tag="VFL49_11885"
     CDS             complement(1675..>3250)
                     /locus_tag="VFL49_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912725.1"
                     /translation="PSAWSPLRQPIFRALWLASVASNIGTWMQNVGAAWLMTSLAPTP
                     AMVALVQAATSLPVFLVGLPAGAVADLVDRRRLLLATQGWMLAAASLLGLLTFMHAIT
                     PWSLLILTFALGLGAAMNAPAWQAILPELVGNDDLKAAVTLNGVGFNVARAVGPALGG
                     LIVAALGSGAVFLLNACSFLGVMFVLYRWPRTASVSTLPAEDVFGAMRAGVRYVRHAP
                     AVQAVLLRTGAFIFGGSALWALLPLVARDELGLGATGYGVILGCLGAGAVAGGLFLPR
                     IERRFSTDALLAGAVVLFALVTAAPVYAAQFALLSVLMIIGGLAWITIMSTFNVAAQT
                     TVPAWVRARALAVYGIVAQGGMAVGSAFWGLVAEHVPLSMTLLLAGTALMLSIAAALR
                     YRLRIDEAIDLTPSEHWPAPVLSMQPAPDAGPVLITVEYIIDAARADEFLRVMRAVRT
                     LRLRDGAYRWGLYSDSSAPTRFVEAFVVESWAEHLRQHSRVTVADKDVWQIALAFHVG
                     PTPPSVSHLIYAGNRR"
BASE COUNT          701 a          993 c         1015 g          541 t
ORIGIN      
        1 aggagacgcg ccgcattcgc tttgccgtca ccaatccgcc gcattttctg ccgatctggc
       61 tcggcagaga cgcggggatt tttgccaagc acggcttgga cgtggaaatc atcttcatgc
      121 gcggcggcgc gctgatcacc atggggatcc tcagcggcga attgcagctc tccggcgtgg
      181 gcgccgaatc cgtagttgcc gccaagctcg aaggcggcga cgctgtgctg cttgcctgtc
      241 cgctggatac cgatctggtc tatttgatcg gccgaccgga tatcaaaacg ccggagcaac
      301 tcaagggcaa gtcaacggcg gtgacgcgcc tgggatcgag cattcatttc tacctgcgcg
      361 ccgcgctcaa atttctcggc atggacgccg acaaagacat gacgattctt caattgggcg
      421 gcgggccgga gatcgcggct gctctagaaa cgggaagaat ttctgcgggt atactcacga
      481 tccgttacgc cttgccgttt ttgcagcgcg gttggccggt gctgatcgac ctgtcgaaaa
      541 ccgatctcaa gtatccgccg tcctgcgtcg gcagcggccg cagcttcgtg cgcaagaatc
      601 taaacaccat agagcgcttt ctcaaagcct acaccgaagc gattcacgtg atcaaaaccg
      661 atggcgcgct ggcgaaacgg gtttacgcca aggaataccg cgaatccgat gcagccgtcg
      721 tcgacaaaat cgtgaccgtt tatgccggac tcttcaagtc ggttcccacc gtgccggcga
      781 gcggtctgga gaccgtgatc cgcgaggcgg cggcgcgcaa acccttgccg agcgacgcct
      841 ttcgccagcc ggacttttat aaagatgacg cgccgctgca gaaaattatt aaagacggat
      901 ggatcgaaca gttgcgtaaa cgctgaggag gtttcatgca gattgaacta gactatcctg
      961 agcccgttcc ggtaaagctc gacccggcca agacgatgct gctgatcgtc gacatggaga
     1021 acgaagacgc gcatccagcg ggcaagcgct acatgggaga agccgtggag gaaattattc
     1081 ccaacatcgc cgcgttgcgc cggcgcgtgc gcgaagcggg cgggctggtc gtgcacacgc
     1141 agtcagtgcg caagcccgac gctttggagt ttacgctctt taaaaacgtg gtcagaaagg
     1201 tggaaggcag ctggggcgcc gagtttattg accagctcaa acccgcatcc ggcgagcccg
     1261 tggtcgtcaa atacacccac gactgcttct atcgaaccga gatggagtcg ctcctggagc
     1321 gcctgcgctt gcgcgccggc gaaggccgca tcatcgtcac cggcatcgcg gcgcgcggct
     1381 gcgtgcagtg cgcggtcatg ggcttcagca tccgcgactt ttacgtctac gtgcccatcg
     1441 attgcacgac gcaaagagat cagaaggaga agctgcaggc attttcgcaa tttgcgagct
     1501 ttggttatcg ttacaacgtc acggtcacgc gctccgattt gattcaattt tagccccgtc
     1561 atttgagttt cgcccgattg atcaccggcg cggcggctcg tggggtcggg attttttccg
     1621 gagtgcttcg cgggcggcga ttgtagccga tcgatcgacg ctgctgctca actctcatct
     1681 gcggtttccc gcatagatta agtgtgagac cgacggcggc gtcgggccga cgtggaaggc
     1741 gagggctatt tgccatacgt ctttgtcggc gaccgtgacg cgcgagtgct ggcgcaggtg
     1801 ctcggcccac gactcgacca caaaggcttc gacaaagcgg gtcggcgcgg aagagtcgct
     1861 ataaagaccc catcgatagg cgccgtcgcg caggcgtaga gtccgcacgg cgcgcatgac
     1921 cctaagaaat tcatccgctc gcgccgcgtc gatgatgtat tcaacggtga tcaataccgg
     1981 acccgcgtcc ggcgccggct gcatcgacag gacgggagcg ggccagtgct ccgaaggcgt
     2041 gagatcaatc gcttcgtcga tgcgcaatcg ataacgcagg gccgcggcta tgctcagcat
     2101 gagcgcggtg cccgccagga gcaaggtcat cgagagcggc acatgctcgg cgaccaaacc
     2161 ccagaaagcg ctaccgactg ccatgccgcc ttgagcgaca atgccgtaga cggccaaggc
     2221 gcgcgctcgc acccaggctg gaaccgtcgt ctgggcggcg acgttgaaag tcgacatgat
     2281 ggtgatccag gccaagccgc cgatgatcat caggacggag aggagcgcga actgcgccgc
     2341 ataaaccggc gcggcagtga ccaaagcgaa gagtacgact gcgcccgcca agagagcatc
     2401 cgtcgaaaaa cggcgctcga tgcgcggtaa aaacaaaccg ccggcgactg cgccggcgcc
     2461 gagacaaccc aaaatcacgc catagccggt ggcgccaagc cccaactcat cgcgcgccac
     2521 caggggcagg agcgcccaga gcgcgctgcc gccgaatatg aatgcgccgg tgcgcagaag
     2581 aactgcttgc acggccggcg catggcgaac atagcgcacg ccggcgcgca tggcgccgaa
     2641 cacatcctcg gccggcaacg tgctcacgct tgctgtccgc ggccagcggt agagaacaaa
     2701 cataacgccg agaaaggagc aggcattgag caggaatacg gcgcccgatc ccagcgcagc
     2761 gacgatgaga ccgccgaggg caggaccgac cgcgcgcgcc acgttgaaac cgacgccgtt
     2821 caaggtgacc gccgccttga gatcatcatt gccgaccaat tcggggagga tcgcctgcca
     2881 cgccggcgca ttcatcgcag cgccaagacc gagcgcgaac gtcaggatca acaaactcca
     2941 gggagtgatc gcatgcatga acgtcagcaa accgagcagg ctggcagcgg ccagcatcca
     3001 accttgagtt gccaacaaca gccggcgccg gtcgacgagg tcagcgacgg cgccggccgg
     3061 caagccgacg agaaaaaccg gcaggctcgt cgccgcttgc accagcgcca ccatcgcagg
     3121 agtcggtgcc agcgacgtca tcagccacgc ggcgccgacg ttttgcatcc atgtgccgat
     3181 gttggacgcg accgaagcga gccaaagggc gcggaagatc ggctggcgca acgggctcca
     3241 agccgatggg
//
LOCUS       DASSRL010000584         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_400444, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000584 DASSRL010000000
VERSION     DASSRL010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400444"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VFL49_11890"
     CDS             <1..1419
                     /locus_tag="VFL49_11890"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HET7912726.1"
                     /translation="RPKPDKVLTDIADYVAGFRITSKEAYNTARLCLMDTLGCGLEAL
                     EYPACTKLLGPIVPGSTVPNGAKVPGTPYQLDPVAAAFNIGTIIRWLDYNDTWLAAEW
                     GHPSDNLGGILATADWLSRTRIARGEKPLVMRDVLTGMIKAHEIQGVIALENSFNRVG
                     LDHVVLVKVASTAVVTHMLGGTRDEVIDALSNAWIDGQSLRTYRHMPNTGSRKSWAAG
                     DATSRAVRLALMTLKGEMGYPSALTAKTWGFYDVLFKGKPFRFKRRYGSYVMENVLFK
                     ISYPAEFHAQTAVEAAIRLHPQVKDRLNDVRKIVITTHESAIRIIDKKGPLHNPADRD
                     HCIQYMTAIGLMKGALTASDYEDDVAADPMIDRLRSKMVCVEHKPWSRDYLNPAKRSI
                     ANGIQVFFSDGTKSERIDVEYPLGHRRRREEGVPQLEAKFRVNLARRLPPKQQNAILA
                     LTRHQERLEATPVNEFVDLFVV"
     gene            1427..1966
                     /locus_tag="VFL49_11895"
     CDS             1427..1966
                     /locus_tag="VFL49_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7912727.1"
                     /translation="MIIRYLSLILLCLAAFAAPAATTDARDPYTYFFNLSTGDLKSDL
                     ADAKTGGRKAVFVMFEQEGCPGCAFMKENVLNRPDVQKFYRDNFVNLSLDIYGSVLVT
                     DFAGRTSTEKGYAQASRVKGTPTLVFYDLSGNEVVRLLGAVKDAREFMLLGEFVASGA
                     YKSRKFAEYKQDLLRKKGS"
     gene            1971..>2360
                     /locus_tag="VFL49_11900"
     CDS             1971..>2360
                     /locus_tag="VFL49_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET7912728.1"
                     /translation="MAYDPFKAIREFKLKSGKTGKYYSLAALEEAGLGKTSRMPISLR
                     VVLESVLRNCDGKRITEDAVKAIAAWKPNAPRTQEVPFVLARIMLQDMAGFPALNDFA
                     AMRAEAKRQKFEPTKIEPLVPVDLVVDH"
BASE COUNT          486 a          781 c          682 g          411 t
ORIGIN      
        1 cgtccgaagc ccgacaaggt tctcaccgac atcgccgact acgtcgccgg ctttcgcatc
       61 accagcaagg aagcatataa caccgcccgg ctgtgcctga tggatacgct gggctgcggg
      121 ctcgaagcgc tggaatatcc cgcctgcacc aagctgctcg gcccgatcgt gcccggcagc
      181 accgtgccga acggcgcgaa ggtgcccggc acgccctacc agctcgaccc ggtcgccgcc
      241 gccttcaaca tcggcacgat tatccgatgg ctcgactaca acgacacctg gctcgccgcg
      301 gaatggggcc atccgtccga caatctcggc ggaattctcg ccacggcaga ctggctttcg
      361 cgtactcgaa ttgcgcgcgg agaaaagccg ctcgtcatgc gcgatgtcct gaccggcatg
      421 atcaaggcgc acgagatcca gggcgtaatc gcgctcgaga acagcttcaa ccgcgtcggt
      481 ctcgaccacg tggtgctggt gaaggtcgcc tccaccgcgg tcgtcacgca catgctgggc
      541 ggcacgcgcg acgaggtcat cgacgcgctg tccaacgcat ggatcgacgg gcagagcctg
      601 cgcacctatc gccacatgcc caataccggc tcgcgcaagt cgtgggccgc gggcgatgcc
      661 accagccgag cggtccgtct cgcactgatg acgctgaaag gcgagatggg ctatccgtcc
      721 gccctcacag cgaagacctg gggcttctac gacgtgctgt tcaagggcaa gccctttcgc
      781 ttcaagcgtc gctacggctc gtatgtcatg gagaacgtgc tgttcaagat ctcctatccg
      841 gccgaatttc acgcgcagac cgcggtagag gcggcgatcc ggctgcatcc tcaagtgaag
      901 gaccgcttga acgacgtcag gaagatcgta atcaccaccc acgagtcggc gatccgcatc
      961 atcgacaaga agggcccgct ccacaatccc gccgatcgcg accactgcat ccagtacatg
     1021 acggcgatcg gcctgatgaa gggagccctc accgcctccg attatgaaga cgacgtcgcc
     1081 gctgacccga tgatcgatcg cctgcgctcg aagatggtct gcgtcgagca caagccgtgg
     1141 agccgtgact acctcaaccc ggcgaagcgc tccatcgcca acggcatcca ggtgtttttc
     1201 agcgacggaa cgaaatcgga gcgcatcgac gtcgaatacc cgctcggcca tcgccgccgt
     1261 cgcgaggaag gcgtcccgca actggaagct aaattccggg tgaatctggc gcgccgcctg
     1321 cctcccaagc agcagaatgc gatactggcg ctcacccgcc atcaagagcg gcttgaagca
     1381 accccggtca atgaattcgt cgacctgttt gttgtctgat ttccatatga taatcagata
     1441 tttatcgcta attctcctct gtctcgctgc cttcgcggcg ccggcagcca cgaccgatgc
     1501 ccgcgacccc tacacgtatt tcttcaatct ctcgaccggc gaccttaaat ccgatctcgc
     1561 cgatgcgaag accggcggac gcaaggcggt gttcgtaatg ttcgagcagg aaggctgccc
     1621 gggctgcgcg ttcatgaagg aaaacgtgct gaaccggccc gacgtgcaga agttctatcg
     1681 cgacaatttc gtcaacctgt cgctcgacat ctacgggtca gtattggtaa ccgatttcgc
     1741 cggccgcacc tcgacggaaa aaggctacgc gcaagcctcc cgcgtgaagg gcacgccgac
     1801 gctggtcttc tacgacctct cgggcaacga ggtcgtccgc ctgctcggcg cggtgaagga
     1861 cgcccgggaa ttcatgctgc tcggcgaatt cgtcgcgagc ggcgcctata aatctcgcaa
     1921 attcgcggaa tacaagcagg acctgctacg aaagaaaggg agttagtcag atggcatacg
     1981 atccgttcaa ggcgatccgg gaattcaagc tcaagtccgg caagaccggg aaatattatt
     2041 cgctcgcggc cctcgaggaa gcgggcctcg gcaagacgtc gcggatgccc atcagcctgc
     2101 gcgtcgtgct cgagtcggtg ctccgcaact gcgacggcaa gcgcatcacg gaagacgccg
     2161 tcaaggcgat cgcggcctgg aagcccaatg ccccgcgcac ccaggaagtg cccttcgtcc
     2221 tcgcgcgcat catgctgcag gatatggccg gcttccctgc cctcaacgat ttcgccgcca
     2281 tgcgcgccga agccaagcgc cagaagttcg agccgaccaa gatcgagccg ctggtccccg
     2341 tggatctcgt cgtcgaccac
//
LOCUS       DASSRL010000585         7707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_977862, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000585 DASSRL010000000
VERSION     DASSRL010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977862"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..503)
                     /locus_tag="VFL49_11905"
     CDS             complement(36..503)
                     /locus_tag="VFL49_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7912729.1"
                     /translation="MTQKFTLNVNGIVHTVDADPDMPLLYALRDNIGLNNPHFGCGLA
                     QCGACTVHLDGQPARSCVTTMSSVGNAKITTLAGLGTPDKPHPLQTAYIEEQVPQCGY
                     CINGWLMTAAAFLRDKKKPTEAEIKDALSGLKCRCGTHISILKAVKRAAEMMG"
     gene            862..1884
                     /locus_tag="VFL49_11910"
     CDS             862..1884
                     /locus_tag="VFL49_11910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004691143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7912730.1"
                     /translation="MKHIGFLSFGHWTPSPQSQTRTASDALLQSIELAVAAEALGADG
                     AYFRVHHFARQLGSPFPLLAACGAKTRRIEIGTAVIDMRYENPMYMAEDAGAADLIAG
                     GRLQLGISRGSPEQVIDGFRYFGYVPPEGMTDADMARRHTEVFLELLLGKGFAKPNPS
                     PMFPNPPGLLRLEPYSEGLRERIWWGAGTNATAQWAARLGMNLQSSTLKIDESGKPFH
                     VQQAEQIRLYRAAWKEAGHARTPRVSVSRSIFALIDDRDRMYFGRGGKEGDQIGMLSA
                     TERAIFGRSYADEPDKLIAQLKGDEAIAEADTVLLTVPNQLGVDYNAHVMEAILKHVA
                     PGLGWR"
     gene            complement(1921..2532)
                     /locus_tag="VFL49_11915"
     CDS             complement(1921..2532)
                     /locus_tag="VFL49_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET7912731.1"
                     /translation="MPNHYDVTTITVRPNTQPKALSILKDQLANTTGLLACWSTDIGA
                     LNRIMIIRAIADVAANLNEHRALLVSRNPFGIGELIDEMAMDTYTALDCVGPIAPGEM
                     GPFYEVRTYVFRPDGVVPTEELWREWLPKRATVSPILTAMMSATGKVARFMHIWPYRS
                     LDERARLRAKAVADKVWPPPGGPEHFVKMQNDIYMPASFSPLR"
     gene            2719..3975
                     /locus_tag="VFL49_11920"
     CDS             2719..3975
                     /locus_tag="VFL49_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912732.1"
                     /translation="MKRKPFLFAAAMAVATLLYAGGPAVAAETVRVGITWPLTGNAGA
                     AGQASKAAAELVAEIVNNPHPELGDLPLAKGAGLPNLGGAKLELIFTDHQGNPSLAQQ
                     LTTRLITQDKVHVMLGAYQSSCSFTATAVAERYGIPFMVGESAALNITGRGFKWVFRG
                     TPIASDYARTYMRFFDDMKKAGKKIDSIAIVNENTDYGTSVAESVTEEAKKANIAVAI
                     RIPYSASSTDVSAQVLQLKEKNPDVVIFISYTADSILYMKTMKNLNYKPPMVIGDDSG
                     FSDPSFVPAVFDVAQGVMNRSAWAIGKPESTTYKINEMYKAKTGRDLDDTSARNMQSF
                     FALVDAINRAGSTDPEKIRVALTQTDLKPEQLMMGYQGVKFDGTGQNILAATYLIQLH
                     GKKYELVWPEKAADARLQWPMTGWTK"
     gene            3979..4836
                     /locus_tag="VFL49_11925"
     CDS             3979..4836
                     /locus_tag="VFL49_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912733.1"
                     /translation="MLLLQVVVGGLLLGAIYALFSSGLTLIWGMMNVINFAHGEFVML
                     GMYVALLVVVWLGGGPAVFGMAAAIALFVLGVVVYAGLIRHVMRGPMLAQILSTFGLA
                     LLLRYTAFWIFSANFVSLPETSLSGTINLGGILLPVAQLVAGVVAIAFTIALHLLLTR
                     TNIGSRLLAVSEDRNAAMLMGIRPDRMQAIAWGLAGSAAGIAGALMATAYPWSPSVGE
                     TFGLIAFVVVAFGGFGSVPGALYAGLIIGLIQAVSAFWFGPIYKDIVVYGLFVALLWL
                     RPQGLMGKA"
     gene            4833..5828
                     /locus_tag="VFL49_11930"
     CDS             4833..5828
                     /locus_tag="VFL49_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912734.1"
                     /translation="MKSVQTNWSVVVLGACLLAYPMIFSGAFYLDIGVTFLLAAISAS
                     AWNIVGGYAGQVSVGHSVFYGFGAYCPLLFYQLFGWPPMAGIPVGIALSIVVAILIGM
                     PTFRLSGHYFSMATIAVAELIRIFTGTWDLVGAAIGLQGPAVARGWWDLTFRGELPYY
                     YIFLVVLAVLLFITWLMEQRRFGYYLRAIKTNERAARSLGVPVLRTKIKALAVSAVFT
                     SIAGSLYSIKTGFIDPESGFGILVSVQMVIIAALGGAGTLFGPLVGALILIPLQTATN
                     TWFGGGGSGLTFILYGGIIVLIARFEPGGLFDLWDRIAPKRWNERWKWWRRGHAA"
     gene            5818..6558
                     /locus_tag="VFL49_11935"
     CDS             5818..6558
                     /locus_tag="VFL49_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912735.1"
                     /translation="MLLEAKNVSKAFGNFRAVAGADLTVDTGDIIGLIGPNGAGKSTF
                     FNCIAGDMQVTTGQIVFDSHDVTHASPEAHAVAGIGRTFQVPATFEDMTVLDNVMVGA
                     FLRHPHRNDAREHAKTILELTGLSRFAHQRARSLGTPGRKRIEIARVLATTPRLMLLD
                     EALAGLTPTELLEAIALVRKIHEAGVTIVIVEHIMEVIMTLTRRVLVFNQGHVIASGK
                     PDDVVRNEAVIEAYLGRGHHTRRARPEQ"
     gene            6572..7312
                     /locus_tag="VFL49_11940"
     CDS             6572..7312
                     /locus_tag="VFL49_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912736.1"
                     /translation="MSNLVIEHLEVRYGDLVGVSDISLKVEAGTIVALLGSNGAGKTT
                     TLNAIAGLVRPSLGSVNWRGEEIAGQPAYQIVSKGLALSPEGWRLFVAQTVEQNLRLG
                     ATTLADKARIESLFERVYTLFPRLAERRRQLAGTLSGGERQMLALGRALMSEPKLLML
                     DEPSLGLAPAVVEALYDMLQRLHEEGLTLLLAEQSIPLALGIADYAYVLQTGRIALQG
                     KASELENNQQVREIYLGISARPASAGLR"
     gene            7342..>7707
                     /locus_tag="VFL49_11945"
     CDS             7342..>7707
                     /locus_tag="VFL49_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7912737.1"
                     /translation="MAARRSAPAPFTPVKGGRKSDQVFEQIAGFIRSGRFPADSRLPA
                     ERELTTMFNTSRQTIREAIYRAELAGLIEVRHGAGSFVVPRKSNTEKTVGELIKIEEG
                     KVGEFFEIRRALEGWCASQA"
BASE COUNT         1390 a         2352 c         2449 g         1516 t
ORIGIN      
        1 cgacattttt tcatgttttg tcataatacc ctccctcaac ccatcatctc ggcggcacgt
       61 ttgaccgctt tcagaatgct gatgtgcgtg ccgcaacgac acttgagacc agagagcgcg
      121 tccttgattt cggcctccgt cggctttttc ttgtcgcgga ggaaagcggc ggccgtcatg
      181 agccagccat tgatgcagta gccgcattgc ggcacctgct cctcgatata ggcggtctgc
      241 aacggatgcg gtttatccgg cgtgccaaga cccgcgagcg tcgtgatctt ggcgttgccg
      301 acagaagaca tggtcgtcac gcatgagcgg gcgggctggc cgtcgagatg aacggtgcag
      361 gcgccgcatt gcgccaggcc gcatccgaaa tgcggattgt tgaggccgat attgtcgcgc
      421 aaggcataaa gcagcggcat gtcgggatcg gcatcgaccg tatggacaat accgttgacg
      481 ttgagggtga acttttgcgt catgcgggtc gctctccggg cttgagcggt ttagccgcgc
      541 tgtcgtggat gttcgcggcg ggtcgttagc ggcgttccaa acacaaagcc taggcaagta
      601 gacccaggtt tgaaacggca aaaggcatta aatttctgtc tcgggttccg ctacgtcgag
      661 gtgggagaca ggttgtcgcc gggttcgggt caggcgcgta actggccggc tattcgatta
      721 ccgtaacgcg gagcaccagt ctggtgttgt tgcgtccctt gaaattgccg aaaggccggc
      781 atcgtgccgg gaatccgcag aggcatggta caagcgcacg acgacagcag ccaacctcgt
      841 cgtcgtgcca ggcgccaccc cgtgaaacac atcggctttc tctctttcgg ccattggacc
      901 ccgtcgccgc agtcgcaaac gcggacagcg tcggacgccc tgctgcaatc gatcgagctt
      961 gcagttgcgg ccgaggcgct cggtgccgac ggggcctatt tccgcgttca tcatttcgcc
     1021 cgccaactcg gctcgccgtt cccgctgctc gcggcctgcg gtgcgaagac caggcgcatc
     1081 gagatcggca ccgcggtcat cgacatgcgc tacgaaaatc cgatgtacat ggcggaggat
     1141 gccggcgccg ccgacctgat cgccggcggc cgcctgcagc tgggcatcag ccgcggttca
     1201 cccgagcagg tgatcgacgg cttccgctac ttcggttacg tgccgccgga gggaatgacc
     1261 gacgccgaca tggcgcggcg ccacaccgag gtcttcctcg aactgttgct cgggaagggt
     1321 ttcgccaagc ccaatccgag tccgatgttt ccgaatccgc ccggcctcct gcgtctcgag
     1381 ccttattccg aggggctgcg cgagcgcatc tggtggggcg ccggcaccaa tgcgacggcg
     1441 caatgggcag ccaggctcgg catgaacctg caaagctcga cactcaagat cgacgagagc
     1501 ggcaagccgt tccatgtgca gcaggcggaa cagatcaggc tctatcgcgc ggcgtggaag
     1561 gaggcggggc acgcgcgcac gccgcgggtg tcggtctccc gcagcatctt cgcgctgatc
     1621 gacgaccgcg accgcatgta tttcgggcgc ggcggcaagg agggtgacca gattggcatg
     1681 ctcagcgcaa ccgagcgcgc cattttcggc cgcagttatg ccgacgaacc ggacaagctc
     1741 attgcgcagc tcaagggcga cgaggcgatc gcggaagccg atactgtgtt gctgactgtg
     1801 ccgaaccagc tcggcgtcga ctacaacgcc cacgtcatgg aggcgatcct gaagcatgtc
     1861 gcgccagggc tcggctggcg gtgaggtatc ttcgttcaat tgccggacgc agctttcaaa
     1921 tcagcgcagg ggcgagaagg acgccggcat atagatgtca ttctgcatct tgacgaaatg
     1981 ctccggccca cccggcggcg gccacacctt gtcggcgacg gccttggctc gcaggcgcgc
     2041 gcgctcgtcg aggcttcgat agggccagat gtgcatgaag cgtgcgacct tgcctgttgc
     2101 cgacatcatc gccgtcagga tcggtgagac cgttgcgcgc ttcggcagcc attcacgcca
     2161 cagctcttcg gtcggcacga cgccgtccgg cctgaacacg taggtgcgca cctcgtagaa
     2221 cgggcccatc tcgcccggcg cgattggccc aacacaatca agcgcggtgt aggtgtccat
     2281 cgccatctcg tcgatcagct cgccgatgcc aaatggattg cgcgaaacca gcagcgcacg
     2341 gtgctcgttc aaattcgcgg cgacgtcggc aatcgcgcgg atgatcatga tccggttgag
     2401 cgcgccgata tcggtcgacc agcacgccag caagccggtc gtgttcgcca gctggtcctt
     2461 caggatggac agcgctttcg gctgcgtgtt cgggcgcacg gtgatggtcg ttacgtcata
     2521 atgattgggc acgttcgctc ctcggattcc gccggattgc agtgttgcac aattgacagc
     2581 ccttacaggg ctttctagcg tggtcagacc actttcttac gctagtggag accgctcgcc
     2641 cacacaatgc cggacccaga cgcgcaaaag atcaggcccg gtctgacaaa gccaatcaaa
     2701 acccgccggg agggaccctt gaaacgcaaa ccgttcctgt tcgccgcggc tatggctgtg
     2761 gctacgttgc tttacgccgg cggtcctgcc gtcgccgccg agaccgtcag ggttggcatc
     2821 acatggccac tcaccggcaa tgccggcgcc gcaggccagg cgtcgaaggc ggcggccgag
     2881 ctcgttgccg aaatcgtcaa caacccgcat cccgagctcg gcgatctgcc gctcgccaag
     2941 ggtgcgggcc tgcccaatct cggcggcgcg aaactcgaac tgatcttcac cgatcaccag
     3001 ggcaatccct cgctcgcgca acagctcacc acccgcctga tcacgcagga caaggtgcac
     3061 gtgatgcttg gtgcgtacca gtcgtcgtgc agcttcacgg cgaccgcggt cgccgagcgc
     3121 tacggcattc ctttcatggt cggcgaatcg gctgcgctca acatcaccgg gcgcggattc
     3181 aaatgggtgt tccgcggcac gccgatcgcg agcgactatg cccgcaccta catgcgcttc
     3241 ttcgacgata tgaaaaaggc cggcaagaag atcgattcga tcgcgatcgt caacgagaac
     3301 accgattatg gcacctcggt cgcggaatcg gtcaccgaag aagcgaagaa ggcgaatatc
     3361 gcggttgcga tccgcatccc ctacagcgcg agttcgaccg atgtctccgc gcaggtgctg
     3421 cagctgaagg agaaaaatcc cgacgtcgtg attttcatca gctacaccgc ggattcgatc
     3481 ctttacatga agacaatgaa gaatctgaat tacaagccgc cgatggtgat cggtgacgac
     3541 agcggctttt ccgatccgtc tttcgtcccg gcggtgttcg atgtcgcaca gggcgtgatg
     3601 aaccgcagcg cctgggcgat cggcaagccg gaatcgacca cctacaagat aaacgagatg
     3661 tacaaggcca agacgggccg cgaccttgat gacaccagtg cgcgcaacat gcagagcttt
     3721 tttgcgctgg tcgatgcgat aaaccgcgcc gggtccaccg atcccgagaa gatccgggtt
     3781 gccctgacgc aaaccgatct caagcccgag cagctgatga tgggatacca gggagtcaaa
     3841 ttcgacggca cggggcagaa cattctggcc gcaacctacc tgatccagct gcacgggaaa
     3901 aagtatgagc tggtctggcc ggagaaggcc gcggacgcca ggctgcagtg gccgatgacg
     3961 ggctggacga aataaccaat gctcctgctg caagttgtcg tgggtggtct cctgctggga
     4021 gccatctacg ccctgttttc ctcgggtctg acgctgatct ggggcatgat gaacgtcatc
     4081 aatttcgcgc atggcgagtt cgtcatgctc ggaatgtatg tcgcgctgct ggtcgtggtg
     4141 tggcttggcg gcggtccggc cgtgttcggc atggcggcgg caatcgcgct ctttgttctc
     4201 ggtgtcgtcg tctatgccgg gctgatccgc catgtgatgc gcggcccgat gctggcgcag
     4261 attctctcca cattcgggct tgctctgctg ctgcgctaca cggcgttctg gattttctcg
     4321 gccaacttcg tctcgctgcc ggagacctcg ctgtcgggca cgatcaatct cggtggcatc
     4381 ctgctgccgg tcgctcagct tgtcgcgggc gttgtggcca tcgctttcac cattgccctt
     4441 cacttattgc tgacgcgcac gaatatcggc agccggttgc tcgcggtctc ggaagaccgc
     4501 aatgctgcca tgttgatggg catccggccc gaccgcatgc aggcaatcgc ctggggtctg
     4561 gcgggtagcg ctgccggcat cgccggcgcg ctcatggcga cggcctatcc atggtcaccc
     4621 tcggtcggcg agaccttcgg tctgatcgcc tttgtcgttg ttgcgttcgg cggctttggc
     4681 agcgtgccag gcgcgcttta cgccgggctg atcatcggcc tgatccaggc cgtatcggcg
     4741 ttctggttcg gcccgatcta caaggacatc gtcgtctacg ggttattcgt cgcgctgctc
     4801 tggctgcggc cgcaaggcct gatgggcaaa gcgtgaaaag cgtccagacc aactggagcg
     4861 tcgtcgtgct cggcgcgtgc ctgctggcct atccgatgat tttttcgggc gcattctatc
     4921 tcgacatcgg cgtgacattc ctgctcgcgg cgatctcggc atccgcgtgg aatatcgtcg
     4981 gcggctatgc cgggcaggtt tcggtgggcc acagcgtctt ctacggtttc ggagcctact
     5041 gcccgttgct gttctatcaa ttgttcggtt ggccaccgat ggcgggcatt ccggtcggca
     5101 tcgccctcag catcgtggtg gcaattttga tcggcatgcc gacgttccgg ctgagcggcc
     5161 actatttcag catggcgacg atcgcggttg cggagttgat ccgcatcttc accggcacat
     5221 gggacctggt gggagcggca atcggcctgc agggcccggc ggtcgcgcgc ggctggtggg
     5281 acctcacctt ccgcggcgag cttccgtact actacatttt tctcgtggtg ctcgccgtgc
     5341 tgctgttcat cacctggctg atggagcagc gcaggtttgg ctattacctg cgcgccatca
     5401 agaccaatga gcgagccgcg cgcagcctcg gcgttcctgt cttgcggaca aagatcaagg
     5461 ccctagcggt gagcgctgtg ttcacgtcga tcgccggatc gctttattca atcaaaaccg
     5521 gcttcatcga tccggaaagc ggttttggca ttctcgtctc ggtgcagatg gtgatcatcg
     5581 ccgcgctggg cggcgccggc acgctgttcg ggcccctggt cggcgcgctg atcctcattc
     5641 cgttgcagac ggcaaccaat acctggttcg gcggcggtgg ctcgggactg accttcattc
     5701 tctatggcgg aatcattgtc ctgatcgcgc gcttcgagcc ggggggcctg ttcgacctct
     5761 gggaccgaat tgcgcccaag aggtggaacg agaggtggaa gtggtggagg cgaggccatg
     5821 ctgcttgagg cgaaaaacgt ctcaaaggct ttcggcaact tccgtgccgt cgccggcgcc
     5881 gatctgaccg tcgataccgg cgatatcatc ggcctgatcg gtccgaacgg ggcagggaag
     5941 tcgaccttct tcaattgcat tgcgggcgac atgcaggtca caaccggcca gattgttttc
     6001 gatagccacg acgtgaccca cgcttcgccc gaagcccacg cggttgccgg catcggccgc
     6061 accttccagg tgccggcgac gttcgaagat atgacggtac tcgacaacgt gatggttggc
     6121 gcgttcctgc gccatccgca ccgcaacgat gcgcgcgagc acgccaaaac catcctcgag
     6181 ctgaccgggc tgagccggtt cgctcatcag cgggcacgct cgcttggcac gcccgggcgc
     6241 aagcggatcg agatcgcccg tgtgcttgcg acgacgccac gcctgatgct gctcgacgag
     6301 gcactcgcag gcctgacgcc gactgagctg ctcgaagcca tcgcgctggt tcgcaagatc
     6361 cacgaagccg gcgttaccat tgtgatcgtc gagcacatca tggaagtgat catgactttg
     6421 accaggcgcg ttttggtgtt caaccagggt catgtgatcg cgagcggcaa gccggatgac
     6481 gttgtcagga acgaggccgt catcgaggcc tatctcggcc gcggccacca caccaggcgt
     6541 gcaagacccg agcaatgaca cgagcaatga catgagcaat ctcgtgatcg agcatcttga
     6601 agtgcgctac ggcgatctgg tcggcgtctc cgatatttcg ctgaaggtgg aggcgggcac
     6661 catcgtcgcg ctgcttggct cgaacggcgc ggggaagacc acgacactga atgcaattgc
     6721 cggcctggtg cggccttccc tgggcagcgt caactggcgg ggcgaggaaa tcgccgggca
     6781 gccggcctat cagatcgtca gcaaaggcct cgctttgtcg ccggaaggct ggcggctttt
     6841 cgtagcgcag acggtcgagc agaacctgcg gctcggcgcg acgacgctcg ccgacaaggc
     6901 tcgcatcgag agcttgttcg agcgggttta tacgcttttc cctcgtcttg ccgagcggcg
     6961 ccggcagctc gccggcacct tgtcgggtgg cgagcggcag atgctggccc tggggcgggc
     7021 actgatgagc gagccaaaat tgctgatgct tgacgagccc agcctcgggc tggcgcctgc
     7081 ggtggttgag gcgctctacg acatgctcca gcgcctgcat gaagagggcc tgaccttgtt
     7141 gctggccgag cagtcgattc cgctggcgct tggtatcgcc gactatgcct acgtgctgca
     7201 gaccgggcgg atcgccctgc aaggcaaagc gtcggagctc gagaacaacc agcaggtccg
     7261 tgaaatctat ctcggcatca gcgcacgccc ggcaagtgcc ggcttgcgct agcgggggcg
     7321 acaatggcga gggcgaaagg cttggctgca cggcgatcgg cgccggctcc tttcaccccg
     7381 gtcaagggcg gacgcaaatc cgaccaggtg ttcgaacaga tcgccggctt catccgttcc
     7441 ggccgtttcc cggccgacag ccggttgccg gccgagcggg agctgaccac gatgttcaac
     7501 accagccggc aaaccattcg cgaggcgatc tatcgcgccg aactggccgg attgatcgag
     7561 gtgcgccatg gcgccggcag tttcgttgtg ccgcgcaagt cgaacacgga aaaaaccgtc
     7621 ggcgaactga tcaagataga ggagggaaag gtcggtgagt ttttcgagat caggcgggcg
     7681 ctggaaggct ggtgcgccag ccaggct
//
LOCUS       DASSRL010000586         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1048041, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000586 DASSRL010000000
VERSION     DASSRL010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048041"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2794)
                     /locus_tag="VFL49_11950"
     CDS             complement(<1..2794)
                     /locus_tag="VFL49_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7912738.1"
                     /translation="MQDADLARELNEALHQQTATAEILKLISSSPADTQPVFDAIVRS
                     GLKLFPNAAIFIALPDGDMLRPAAFVATDPDRAEMWARQWPIPLAREYMHSLAFLDRK
                     TVDIPDAQEAPPELAVGAKNFLPTGYRALTIMPMLRGSTAIGTLSVVRPAPGPLSDRQ
                     VAALRSYVDQAVIAIENTRLLNELRQRTEDLSESLEQQTATSEVLNVISSSPGVLQPV
                     FQVILENATHICGARIGILFRYEDGAYTAVATLGVTPAYAEYLNSGPIRPGPTTGLGR
                     VASTKQTVNVPDTQAETAYAEREPLRVATAELGGARSLLNVPMIKEGELIGAIGIYRQ
                     EVRPFTEKQIELVTNFANQAVIAIENARLLNELRQRTDDLTESLEQQTATSEILEVIS
                     NSPTDTQPAFDAIVRSGLNLFPDAVVTISLPDRDLVKLGAIGGADEAGVESLRGRFPM
                     PLSHEFITGTAILDRREIDLADAHEPPKELTAGAQNLLAGGYRAMTVMPMVRGDETIG
                     TLNVVRRNPGPLSDKQRELLRTFANQAVIAIENTRLFNELRESLQQQTATADVLKVIS
                     RSTFDLQTVLDSLVESAARVCEADSGIIRRREGDIYPVAATFGLTAEQRDHFARYSTN
                     PDRGSVYGRAIVEGRTIHVPDLLVDPELDRDRLRDYAGVINIRGGLGVPLMRDGTIVG
                     VFTLQRREPRPFTDKQIELVETFANQAVIAIENVRLFNETREALERQTATSEVLKVIS
                     SSPGELEIVFRTLLANATRICQAAFGSMLQLDGGMFRRVALHNAPAAFAEFHARAPLV
                     DPQQVSDLKRVIETKQVVHVADTAAEYPDAPIAKYAGGRTLLIVPMLKDEELIGAIGI
                     YRQEVRPFTEKQIDLVKNFAAQAVIAIENTRLLNELRQRTDDLSESLERQTATSEVLK
                     VISSSPGNLQPVFEAML"
BASE COUNT          523 a          855 c          884 g          562 t
ORIGIN      
        1 cgagcatggc ctcgaacacc ggctgcaaat tgcccggcga actggagatg actttcagca
       61 cctcgctggt cgccgtctgc cgctccagcg actcagagag gtcatcggtg cgctggcgca
      121 attcgttgag cagccgcgtg ttctcgatgg cgatgacggc ctgcgcggcg aagttcttga
      181 ccagatcgat ctgcttttcg gtgaacgggc ggacctcctg gcggtagatg ccgattgcgc
      241 cgatcaactc ctcatccttg agcatgggca cgatcagaag ggtgcgaccg ccggcatatt
      301 tggcaatcgg agcgtcggga tactcggccg cggtatccgc aacatgaacg acctgcttgg
      361 tctcgatcac gcgtttgagg tcgcttactt gttgcggatc gacgagcggc gcccttgcat
      421 gaaattcggc aaacgcagcc ggtgcattat gcagcgccac gcgccggaac atgccgccgt
      481 ccagctgcaa catggagccg aaggcggcct ggcaaatgcg agtcgcattt gccagcaagg
      541 tccggaacac aatttccaac tcgccgggcg agctggagat gaccttcagc acctcggagg
      601 tcgcggtctg ccgctccagc gcttcccttg tctcgttgaa cagccggaca ttctcgatcg
      661 cgatcaccgc ctgattggca aatgtttcca ccaactcgat ctgcttgtcg gtgaacggac
      721 gcggctctct gcgctgcagg gtgaagacac cgacaatggt tccgtcccgc atcagcggga
      781 cacccagccc accgcgtata ttgatgaccc cggcgtaatc tcgaagccgg tcccggtcga
      841 gctcgggatc gaccaaaagg tccgggacat gaatcgtgcg gccttcgact attgcgcgtc
      901 catagaccga tccacgatcc gggttcgtcg agtaacgtgc gaaatgatcg cgctgttctg
      961 cggtgaggcc gaaagtggcg gcgaccggat agatgtcacc ctcgcgccgc cggataatcc
     1021 ccgaatccgc ttcgcatacg cgggccgccg actcgaccag cgagtcgagc accgtctgca
     1081 agtcgaacgt cgaccggctg atgaccttga gcacatcggc ggtggcggtc tgctgctgca
     1141 aagactcgcg cagctcattg aacagccgcg tattctcgat ggcgatgacc gcctggttgg
     1201 cgaaggtgcg taacagctcc cgctgtttgt cagagagggg cccgggattg cgccgtacga
     1261 cgttcagcgt gccaatcgtc tcatcgccac gcaccatcgg catcaccgtc atggcgcggt
     1321 agccgccggc cagcagattt tgcgcgcccg ccgtcaattc cttcggcggc tcatgcgcgt
     1381 cggcaagatc gatttcgcgc cggtcaagaa tggctgtgcc tgtgataaac tcgtgcgaca
     1441 gcggcatcgg aaagcgcccg cgcagggatt cgacaccggc ctcgtccgcc ccgccaatag
     1501 cgccaagctt gaccagatcg cggtcgggaa gcgaaatggt cacgaccgcg tccgggaaca
     1561 gattcagccc gctccgcacg atcgcatcga aggccggctg cgtgtcggtc ggcgagttgc
     1621 tgatgacctc gagaatttcc gacgtcgcgg tctgctgctc cagcgattcc gtaagatcat
     1681 cagtgcgctg tcgaagctcg ttcagcagcc gcgcgttttc gatggcgatg acggcctgat
     1741 tggcaaaatt cgtgacgagt tcaatctgtt tttcggtgaa gggacggact tcctgacggt
     1801 agataccgat ggcaccgatc aactcgcctt ccttgatcat tggtacgtta agaagagatc
     1861 gtgcgccgcc aagttccgcg gttgcgacac gcaagggctc tcgctcggca taagccgttt
     1921 cagcctgcgt atccggcaca ttgacggtct gcttggtgct tgcgacccgg cctagccccg
     1981 tcgtcggacc agggcgaatc ggtccgctgt tgagatactc ggcataggcc ggcgtcacgc
     2041 cgagcgttgc gacagccgtg tacgccccat cttcgtagcg gaacagaatt ccgattctcg
     2101 ccccacagat gtgggtcgca ttttccagga tgacctgaaa gaccggctgg agtacgccgg
     2161 gcgaactaga gataacgttc agcacttcgg acgttgcggt ctgctgttcc aatgactcac
     2221 tcaaatcttc cgtgcgctgg cgcagctcgt tgagcagccg cgtgttctcg atggcgatga
     2281 cggcctggtc gacatagctt ctcagtgccg caacttgccg gtcggacagc ggaccgggcg
     2341 cgggtcgcac gacactgagg gtgccaatgg cggtgctgcc gcgcagcatc ggcatgatcg
     2401 tgagtgcgcg gtaaccggtg ggcagaaagt ttttcgctcc aacggcaagt tcggggggcg
     2461 cttcctgggc gtcgggaatg tccacagtct ttcgatcgag aaacgcgagg ctgtgcatgt
     2521 attcgcgggc gagtgggatc ggccactggc gcgcccacat ctctgcacga tcggggtccg
     2581 tcgcgacaaa agcggcgggc cgcagcatgt ctccgtcagg aagcgcaatg aaaatagcgg
     2641 cgttcggaaa gagcttcagc ccgctccgca cgatggcgtc gaaaacgggc tgcgtgtcgg
     2701 ccggcgaact gctgatcagc ttgaggattt ccgcggttgc ggtctgctgg tgcagcgctt
     2761 cgttgagttc gcgggcaaga tcggcatctt gcactttgga tgccgccgcc tggcgcgccg
     2821 gtga
//
LOCUS       DASSRL010000587         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_767847, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000587 DASSRL010000000
VERSION     DASSRL010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767847"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /gene="metX"
                     /locus_tag="VFL49_11955"
     CDS             <1..548
                     /gene="metX"
                     /locus_tag="VFL49_11955"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735222.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET7912739.1"
                     /translation="WRGGRYLLEGSNPRRGLAVARMGAHITYLSDAALHRKFGRKFQD
                     RANPTFSFDADFEVESYLRHQGISFVERFDANSYLYLTRAMDYFDLAADCGGVLAKAF
                     HRSPTRFCVISFTSDWLFPTSESRAIVHALNAASARVSFAEIVTDKGHDAFLLDEPEL
                     FAIVRGFLDGAGKARGLGSGS"
     gene            576..1220
                     /gene="metW"
                     /locus_tag="VFL49_11960"
     CDS             576..1220
                     /gene="metW"
                     /locus_tag="VFL49_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HET7912740.1"
                     /translation="MDLSANRAALATSGPRVDLVLVAEMVERGAKVLDVGCGDGELLR
                     LLSESRDVDGRGIELSREGVNECVAKGLAVIQGDADTDLGDYPDDAFDYVILSQTLQA
                     TRQPREVLEHMLRIGRHAIVSFPNFGHWQVRLQLLFAGHMPRTDILPFSWYNTPNIHH
                     CTIKDFRQLCTEVGVRMEKAVALNAWGSPLRLNAPWWFWNLFGEQAVFLLSRRD"
     gene            1858..2196
                     /locus_tag="VFL49_11965"
     CDS             1858..2196
                     /locus_tag="VFL49_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HET7912741.1"
                     /translation="MLVTEARKYIGTNPTGRGSLWCGAFMDMVLKRTGHRGGGNLAKA
                     YSHYGTRVSGPQVGAIAVMRRRGGGHVGVVSGIDPNGNPIIVSGNHNHTVAESVYPAG
                     RITAYVLPGG"
     gene            complement(2258..3034)
                     /locus_tag="VFL49_11970"
     CDS             complement(2258..3034)
                     /locus_tag="VFL49_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7912742.1"
                     /translation="MEFEAAAVRAIYRYPVKGLSAQPLEQTKLTAGQTIPADRLYAIE
                     NGPIGFDPAAPAYFPKQRFLMLMRNERLAALRTQFDEPTHTLTIRWQDAEAARGDLRT
                     PQGRTAIESFFARYCADELRGPPKVLRGEGHSFSDVAKKVVSIINLASVAAVEEAIGQ
                     PVHPLRFRANIYVSGWPAWREFDLLDQTIAVGNTAQVKIIKRIQRCAATEVDPDRGVR
                     DLAIPRTLLKNFDHTDCGVYAEVVAAGDIATGDAVATAPQ"
     gene            3202..>3895
                     /locus_tag="VFL49_11975"
     CDS             3202..>3895
                     /locus_tag="VFL49_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7912743.1"
                     /translation="MNFEKYTDRARGFIQSAQSLALREGHQQFAPEHLLKVLLDDPEG
                     LAAGLIDRSGGQSRKALEAVDAVLAKRPKVSGGGAGQIYLDPALARVFDAAEKAGEKA
                     GDSFVTVERLLLALALEKDSEAGKILARAGVTPQNLNAAINALRKGRTADSASAENAY
                     DALKKYARDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLALRIVNGDVP"
BASE COUNT          750 a         1146 c         1218 g          781 t
ORIGIN      
        1 aatggcgcgg cggccggtat ctgctggagg gctccaatcc gcgcaggggc ctggcggtgg
       61 cgcgcatggg cgcgcacatt acatatctgt ccgatgcggc gttgcatcgg aagttcggcc
      121 gcaagtttca ggatcgcgcg aatccgacct tctccttcga tgcggatttc gaggtcgaat
      181 cctacttgcg ccatcaggga atttcattcg tcgagcgttt cgacgcgaac tcctatctct
      241 atctgacgcg ggcgatggat tacttcgatc tggcggccga ttgcggcggt gttctggcca
      301 aagccttcca ccgaagcccg acacgcttct gtgtgatctc atttaccagc gactggctgt
      361 ttccgacgtc ggagtcgcgc gccatcgtgc acgcgctcaa cgcggcgagc gccagggtgt
      421 ctttcgccga aatcgtcacc gataaaggtc acgatgcctt tctgctcgac gaacccgagt
      481 tgtttgccat cgtgcgcggg tttctcgatg gggccggcaa ggcgcgcggg cttggttccg
      541 ggagctgacc ggtgagcgcc ctgtcacgca ccgaaataga cctgagcgcc aaccgcgccg
      601 cgctcgcaac aagcggtccg cgcgtcgatc tcgttctcgt tgccgagatg gtcgagcgcg
      661 gcgcaaaggt cctggacgtc ggctgcggtg acggtgaatt gcttcggctt ctgagtgaaa
      721 gccgcgacgt cgacgggcgc ggtatcgagt tgtcgcgaga gggcgtgaat gaatgcgttg
      781 ccaaaggtct ggctgtgatc cagggcgacg ccgataccga ccttggcgac tacccggatg
      841 atgccttcga ctacgtcatc ctgtcgcaaa ccctgcaggc tacccggcag cctcgggaag
      901 tgctggaaca tatgttgcgg atcgggcgcc acgcgatcgt gtcgtttccg aatttcggcc
      961 actggcaggt caggctgcag ctcctcttcg ccggccatat gccacgcaca gatatcctgc
     1021 cgttcagctg gtacaacacg cccaatatcc accattgcac catcaaggat ttccgccagc
     1081 tctgtacgga agtcggcgtc cggatggaga aggccgtggc gctcaatgcc tgggggtcac
     1141 cgctgcggct caacgcgcct tggtggttct ggaacctgtt cggcgagcag gcggtgttct
     1201 tactcagccg ccgcgactag ctcggttccc ttaatatttc tgccggtacg taacgcgggg
     1261 tcgctttaac tgtggcactg cggcgcaata tctttgtctg gaagtataag tcgatccgaa
     1321 aacagtactc ctataattct actaatattc acaattatat cagcgtgtat agcgtcttat
     1381 gaatgggtat tccgatggaa tagcggctat gcgtcgcggg aattgacctt tcaatgatca
     1441 cgaaattgcc atatgggggc ggcgtttgga ctctgcgcct gatggacgat tctatcagtg
     1501 cggcatgtat tgcttacgaa gctaagggga ctactcaaaa tgacgatagg accaacccgg
     1561 ggaaccttat tcctttgtgc gatagcggtt acagcactgg caacgtcggc tgtcgcgaga
     1621 ccggcacata agcatcgcgc tgcgacggcg actcagacag cagttcaacc gcagctgtca
     1681 ggggccgacc gcgatatgcg ctattcgttc aatgcaaatc aggtaccggc ggcaacccgg
     1741 acggctgcgg gcgaaacgac ccgtcatggc cgtcgccatg gccgccggga tcaggcctcg
     1801 gcgcgcgcat tggcccagtc ccatgcaaac tcggcgtttg gcggcgtcac cagcaatatg
     1861 ctggtaacgg aagcgcgcaa atacatcgga accaatccca cgggccgggg aagcctttgg
     1921 tgtggagcgt tcatggacat ggtgctcaag cggacaggcc atcgcggtgg cggcaatctc
     1981 gccaaggcct actcacacta cggcacgcgc gttagcgggc cgcaggtcgg agcgattgcc
     2041 gttatgcgac gccggggcgg cggtcacgtc ggtgtggtga gcggcatcga cccgaacgga
     2101 aacccgatca tcgtgtccgg caaccacaat cacaccgtcg ccgaatcggt gtacccggct
     2161 gggcggatca ccgcctacgt tttgcctggc ggctgatctc gtatcgatta cagcagcctt
     2221 gtcatccgcg ctccgtccag aacggggcgc ggatttttta ttgaggcgct gtggccactg
     2281 catcccccgt ggcgatatcg cccgccgcga cgacttcagc atagacgcca cagtcggtgt
     2341 gatcgaagtt tttcaggagc gtgcgtggaa ttgccaggtc ccggacaccc ctgtctgggt
     2401 cgacctcagt cgctgcgcag cgttgtattc gcttgatgat cttgacctgc gccgtgtttc
     2461 cgacggcaat cgtttggtcg agaaggtcga attcgcgcca tgccggccaa ccggatacat
     2521 aaatgttggc ccggaagcgc agcggatgga ccggctgtcc gatggcctct tcgaccgcgg
     2581 ccaccgaggc tagattaatg atcgacacca cctttttggc gacatccgag aagctgtgtc
     2641 cctcgccacg caagaccttg ggcggcccac gcaactcatc ggcgcaatag cgagcaaaga
     2701 agctctcgat agcggtgcgg ccctgcggag tgcgcaggtc gccgcgcgcc gcctcggcat
     2761 cctgccagcg gatcgtcagg gtatgagtcg gctcatcgaa ctgcgtgcga agcgcggcga
     2821 ggcgctcgtt gcgcatcaac atgagaaacc gttgcttcgg gaaataggcc ggcgcggcag
     2881 ggtcaaagcc aatgggaccg ttctcaatgg catagaggcg gtcggcaggg atggtttgac
     2941 ccgccgtcag cttggtctgt tccagaggct gcgccgatag ccctttgacg gggtagcggt
     3001 aaatggcccg tacggcagcg gcttcgaatt ccattgattt tccggcgcaa aatcgagccg
     3061 caaggatcgt taaggctcac ctatatcagg caattcgccc cttcaaacct caaaaactgc
     3121 acccacatac aaggacgcgg taggctgcct tatgaggggc ctgcccgttt ccggcgccgt
     3181 aacgggccga gggagtgagg catgaatttc gagaaatata cggaccgcgc gcgcggtttc
     3241 atccagtctg cgcaatcgtt ggccttgcgc gaaggccacc agcaatttgc ccccgaacac
     3301 ctgctcaagg ttttgctcga tgatccggaa ggccttgctg ccgggttgat cgatcgctcc
     3361 ggcggtcaat cgcgtaaggc gttggaggcg gtggatgccg tgctcgccaa gcggcctaag
     3421 gtcagcggcg gcggtgccgg ccagatctat ctcgatccgg ctctggcgcg tgtcttcgac
     3481 gccgccgaaa aagccggcga gaaggccggc gacagctttg tgaccgttga acggctgctg
     3541 ctcgcccttg cactggagaa agacagcgag gcggggaaga ttctcgccag ggcgggcgtg
     3601 acgccgcaaa acctgaatgc cgcgatcaat gcgctgcgca aagggcggac ggccgattcc
     3661 gcgtccgccg agaacgccta cgatgcgctg aagaaatatg cgcgcgatct cacgcaggcc
     3721 gcgcgcgaag gcaagcttga ccccgtgatc ggccgcgacg aagagatccg gcgcacgatc
     3781 caggtcctgt cgcgccggac gaaaaataat cctgtgctca taggtgaacc tggcgttggc
     3841 aagacggcga ttgtcgaagg gcttgcgttg cgcatcgtca atggcgatgt gccgg
//
LOCUS       DASSRL010000588         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1625533, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000588 DASSRL010000000
VERSION     DASSRL010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625533"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFL49_11980"
     CDS             <1..595
                     /locus_tag="VFL49_11980"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HET7912744.1"
                     /translation="HVSTDYVFDGTKRAPYLETDAPNPLNVYGATKLAGDRKIESAGL
                     RRWAILRTSWLVSELGDTFPVKILRRAQAGEALRVVDDQQGCPTAASELARAMQQIGL
                     RLLDEDKAACGLFNFCGGAAMSWHDFAEKLLDAAQAAGLKRAPLQAIKSAELKAAARR
                     PAYSVLSCARIRGRCGIAHADIDLEIERMVRSIVARA"
     gene            899..1204
                     /locus_tag="VFL49_11985"
     CDS             899..1204
                     /locus_tag="VFL49_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912745.1"
                     /translation="MTSESLGRKASGSDLSKRIDAMYARDRLWAWGFVVALWLVVGFV
                     LLESIPYASGAVTTVCWIAAIVLLVFNTGSIINMVRHYGHDKERIYGTDIRHLDAGK"
     gene            1217..1639
                     /locus_tag="VFL49_11990"
     CDS             1217..1639
                     /locus_tag="VFL49_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912746.1"
                     /translation="MAKQVYQPPRQSVPGQWIDVTVLLVLIFLALFVPVWAKIAVPSR
                     VEKLPPGVSYVTAEDGTKTWTGLTWEALGQNPTMQQQWEKLGYTVESAADIITMPFDY
                     DFDWAGVIGMIVLIGGYYVFLLWHSEKEYRQVIQEKFD"
     gene            1691..1852
                     /locus_tag="VFL49_11995"
     CDS             1691..1852
                     /locus_tag="VFL49_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912747.1"
                     /translation="MWDALEWIAWIVSAIIFIWMVYDGWRVGQTHREDVLLSSREGVD
                     ELVPEKMRE"
     gene            1856..>2262
                     /locus_tag="VFL49_12000"
     CDS             1856..>2262
                     /locus_tag="VFL49_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7912748.1"
                     /translation="MAEQTMGGQGQPGINLLKVLGPVHVWALGVGIVLVGEFMGWNYA
                     VGKGGALGCLIAMWVIGLLYTCVAMIDSEITSTVAAAGGQYAQAKHTIGPLMAFNVGL
                     YLVFAYTMLEAADVQVVGFLIQIGATDLLGTAPT"
BASE COUNT          424 a          654 c          756 g          428 t
ORIGIN      
        1 ccatgtctcc accgattatg tgttcgacgg cactaaacgg gcgccttatc tcgagaccga
       61 cgcacccaac ccgctcaatg tctacggcgc gacgaagctc gccggcgacc gcaaaatcga
      121 gtcggcaggg ctgcgacgct gggcaatcct gcgcacgtcc tggctggtga gcgagctcgg
      181 cgacaccttc ccggtgaaga tattgcggcg cgcgcaggcc ggcgaagcgc tgcgggtggt
      241 cgatgatcag cagggctgcc cgacggcggc gtccgagctg gcgcgcgcca tgcagcagat
      301 tgggctgcgg ctgctcgatg aagacaaggc cgcctgcggc ttgttcaact tctgtggcgg
      361 cgccgccatg agctggcacg atttcgcgga gaaactgctg gatgcggcgc aagcggccgg
      421 gttgaagcgc gcgccgctgc aggcgatcaa gagtgcggaa ttgaaggcgg cggcgcggcg
      481 tccagcctat tcggtcctat cctgtgcgcg gattcgcggc cgatgcggga tcgctcacgc
      541 ggacatcgac ttggagatcg agcgcatggt ccgttccatt gttgcgcgtg catgacacgg
      601 aagtccgaaa gaccggccca gtgccgatga gatagcagga gatcgcaatt gccatgtcgt
      661 ccgaagcgca gggcgagtcg ccggagcgtt caacgtgacg aatgcgagtc gatgcggcta
      721 cgagcaactt tcttgttgcg gggttgctta ggtgggcata tccttagagg aatcaaaaat
      781 tccgctgagt gaacatcagc ccgaggcgcc ggcgagcagg atcaggggtg gcggagaaat
      841 aacgagttgt tcgccggaaa cgcagcgcca tcggtgctct cgtagtgggg ggttgtacat
      901 gacatcagaa agtctgggcc ggaaggcctc cggatccgat ctgtccaagc gcatcgatgc
      961 gatgtacgca cgcgaccggc tgtgggcctg gggcttcgtc gtcgccctgt ggctggtggt
     1021 cggtttcgtc ctgctggagt ccattccgta cgccagcggc gccgtgacca ccgtatgttg
     1081 gattgccgcc atcgtgctgc tggtcttcaa cacgggctcg atcatcaaca tggtgcgtca
     1141 ctacggacac gacaaggaac gcatctacgg caccgacatc cgtcaccttg acgccggaaa
     1201 gtaggcggag actcacatgg caaagcaagt ctatcagccc ccgcgacaga gcgttcccgg
     1261 gcagtggatc gacgtcaccg ttcttctcgt cctgatcttc ctggcgctat tcgtgccggt
     1321 gtgggcgaaa atcgccgttc cctcgcgcgt ggagaagctg ccgcccggcg tgagctatgt
     1381 cactgccgag gacggcacga agacgtggac cggcctgact tgggaagcgc tcgggcagaa
     1441 ccccacgatg cagcagcagt gggaaaagct cggctacacg gtcgagagcg ctgccgacat
     1501 catcaccatg ccgttcgact acgacttcga ctgggccggc gtcataggca tgatcgtgct
     1561 gatcgggggc tactatgtgt tcctgctctg gcactccgag aaggagtacc ggcaggtcat
     1621 ccaggaaaaa ttcgactgat cccggcagcg gccggcaaag atcggggcag tttcggacag
     1681 gggacagcac atgtgggatg cactcgagtg gatcgcctgg atcgtgtcgg cgatcatctt
     1741 catttggatg gtttacgacg gttggcgcgt gggccagacc caccgcgaag acgtgctgct
     1801 cagctcgcgc gagggtgtcg acgagctggt acccgagaag atgagggagt gagccatggc
     1861 tgagcagaca atgggcggcc agggccagcc tggcatcaat ctcctcaagg ttctgggtcc
     1921 cgttcacgta tgggcgctcg gagtcggcat cgtgctggtc ggcgagttca tgggatggaa
     1981 ctacgccgtc ggcaagggcg gcgctctcgg ctgcctgatc gccatgtggg tgatcgggct
     2041 gctctatacc tgcgtcgcca tgatcgattc cgagatcacc tcgacagtgg cggcggctgg
     2101 cggccagtac gcccaggcga aacacaccat tggaccgctg atggctttca acgtcggtct
     2161 ttacctggtc ttcgcctata cgatgcttga agctgccgat gtacaggtgg tgggtttcct
     2221 gatccagatc ggcgcgaccg acctcctggg gaccgcgcca ac
//
LOCUS       DASSRL010000589         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1695576, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000589 DASSRL010000000
VERSION     DASSRL010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695576"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFL49_12005"
     CDS             <1..787
                     /locus_tag="VFL49_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7912749.1"
                     /translation="SLEGGLVEEIAVQEGDTVQKGQVLARIDNTKFSADLGEVKAKRE
                     AAANSLKLLNREVELTRKLYEQKVVPEIEMLRLQRQATDLSGQIAVFDETIKSAEDRV
                     RRTELRSPVYGIINKINVTTVGAVVQPGANLMDIVPLDDTLLVEGRLRPQDIAFIRPG
                     QDAVVKLTAYDSSIFGSLKGKVERISADTIIDDRSKTGDKPESFYRVMIRTDKNYLGT
                     QENPLPIIPGMVATVEVLTGRKSVLDYLVKPARMLADEAFRER"
     gene            968..1780
                     /locus_tag="VFL49_12010"
     CDS             968..1780
                     /locus_tag="VFL49_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7912750.1"
                     /translation="MNERARPFQLDQWVLPLLADPVTKKHKNAHAFACHEGVIDARVY
                     LRHTPGFTGWSGGQEFYESWQKRTEADYQGEIEGTVPVYDHLAISGRILDVGGSFGTL
                     RHFLPRESEYLSADPFIDCLAYTSAAMRSAYPCLSKPLNLIGACAEFLPLQADAFDWV
                     HMRSVVDHFHSPDLALLEARRVLKPHGKLVVGLYVDGGKSGRRPPGRAIKEALRPLLV
                     AAGFKRFKDHHVFHPTFEGLKELVADCGFHITDVYWQPQWADQVCYVTAERA"
     gene            1777..1998
                     /locus_tag="VFL49_12015"
     CDS             1777..1998
                     /locus_tag="VFL49_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912751.1"
                     /translation="MMDWPSLAWFVAGGLIGGVIGIACTVWAVNYTFGRMYPARRETG
                     SYDKVARPDESAAQGAEAKTAHSGFPLHD"
     gene            2233..>2446
                     /locus_tag="VFL49_12020"
     CDS             2233..>2446
                     /locus_tag="VFL49_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912752.1"
                     /translation="MSAKARERVAAQWLPVVLFLGFVAAAALDTNATAASKIAVVANV
                     AAGEPTVAPQLVQPAPAARTERPPQSF"
BASE COUNT          477 a          777 c          735 g          457 t
ORIGIN      
        1 gtcgcttgag ggtggcttgg tcgaggaaat cgcggttcag gaaggcgaca ccgtgcagaa
       61 gggccaggtg ctcgcccgta tcgacaatac gaaattttcc gccgatctcg gcgaggtcaa
      121 ggcaaagcgc gaagctgccg ccaacagcct gaagctgctg aaccgcgagg tcgagctcac
      181 ccgcaagctc tatgaacaga aggtcgtgcc ggagatcgag atgctgcgct tgcagcggca
      241 ggcgaccgac ctctccggcc agatcgccgt cttcgacgag accatcaagt cggcggaaga
      301 tcgcgtgcgc cgcaccgagc ttcgctcgcc ggtctacggc atcatcaaca agatcaacgt
      361 caccacggtt ggcgccgtgg tgcagccggg cgccaacctg atggacattg tgccgctcga
      421 cgacaccctg ctggtggaag gccgactgcg tccgcaggac atcgccttca tccgccccgg
      481 ccaggacgcc gtggtgaagc tgaccgccta tgattcatcg atcttcgggt cgctcaaggg
      541 caaagtcgag cgcatcagcg ccgacaccat catcgatgac aggtcgaaga ccggcgacaa
      601 gccggagagc ttctaccggg tgatgatccg caccgacaag aattacctcg gcacgcagga
      661 aaacccgctg ccgattatcc ccggcatggt ggcgaccgtc gaggtgctca ccggccgcaa
      721 gtcggtgctc gactatctcg tcaagccggc gcgaatgctc gccgacgaag cgttccggga
      781 acggtaggcg attttcttcg ctcttcggtg tgaaacctcc gttcattccc gcgaaagcgg
      841 gaatccagaa tacgacgcca agatttgtag cctgctgttg gacccccgct tccgcggggg
      901 gtgagcggaa ttgtggttcc tcatagccgg acttgtcggc atgaaccgct tgtgagacga
      961 ttccggcatg aacgagcgag cgcgcccctt tcaactcgat cagtgggtgt tgccgctcct
     1021 tgccgatccc gtcaccaaga aacacaaaaa cgcgcatgcc ttcgcttgtc atgagggcgt
     1081 catcgatgcg cgcgtttacc tgcgccacac gcccggattc accggctggt cgggaggaca
     1141 ggaattctat gagagctggc aaaaacgcac cgaagccgat tatcagggcg agatcgaagg
     1201 gaccgtcccc gtctacgacc acctggccat ctccggacgg atccttgatg tcggcggcag
     1261 cttcggcacg ctgcggcact tcctgccgcg ggagagcgaa tatctctccg ccgacccttt
     1321 tatcgattgc ctcgcttaca ccagcgccgc catgcgaagc gcctatccct gcctgtcaaa
     1381 gccgctgaat ttaatcggcg cctgcgccga attcctgccg ctgcaggccg acgccttcga
     1441 ctgggtccac atgcgctcgg tggtcgatca cttccacagc cccgatctgg cgctgcttga
     1501 ggcgcggcgc gtgctcaagc cgcacggaaa acttgtcgtc ggcctctatg tcgacggcgg
     1561 aaaatcggga cgccgaccac ctggtcgcgc gatcaaggaa gccctgcgtc ctcttctggt
     1621 cgcggcgggc ttcaaacgat tcaaagatca ccacgtcttt catccgacct tcgagggcct
     1681 caaagaactt gtcgccgact gcggcttcca catcaccgac gtttactggc aaccgcagtg
     1741 ggccgatcag gtctgctacg tcacggcgga gcgcgcatga tggactggcc gtcgctcgcg
     1801 tggttcgtgg ccggagggct cattggcggt gtcatcggca tcgcctgcac ggtatgggcg
     1861 gtcaactaca cgttcgggcg catgtatccg gcacggcgcg agacagggtc gtatgacaag
     1921 gtcgcgcgcc cggatgagtc tgctgcacaa ggcgccgaag cgaaaacggc gcattctgga
     1981 ttcccgctgc acgattgacg gaaaaatgat tcaaatttta attgctacaa ttttacataa
     2041 ctcaattttc gccgattttt agccgccgcg tttcacccgc tcttacggtg actccgacga
     2101 aaccggcact ttccccgtcc atttaactgg cccgaagcag cgcgggccgt atcgctagcc
     2161 ccggaaaaaa agatccggag cgggcggcat gtcacttcgc cgtttcaacg ccccggacgc
     2221 ggggtgtcga acatgagtgc gaaggcgcgg gagcgcgtag cggcacagtg gctgccggtc
     2281 gtgctgtttc tcggttttgt cgcagcggcg gccctggaca ccaatgcaac ggcggccagc
     2341 aagattgccg tcgtagcgaa cgtcgccgcg ggtgagccaa ccgttgcccc gcagctcgtt
     2401 cagccggcac cggccgcacg caccgagcgc ccgccgcagt ctttcg
//
LOCUS       DASSRL010000590         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1328181, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000590 DASSRL010000000
VERSION     DASSRL010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328181"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFL49_12025"
     CDS             <1..192
                     /locus_tag="VFL49_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912753.1"
                     /translation="KNPIGVKGSGECGVTGSIPAVLNAVNDALARAGASTAIGLPVTP
                     EKIWRCLRDGRALKAKRRR"
     gene            complement(253..328)
                     /locus_tag="VFL49_12030"
     tRNA            complement(253..328)
                     /locus_tag="VFL49_12030"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Glu,seq:ctc)
     gene            complement(389..730)
                     /locus_tag="VFL49_12035"
     CDS             complement(389..730)
                     /locus_tag="VFL49_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912754.1"
                     /translation="MVQGMSGDSEKLLDPEAARVVAKVRRLMMIATMTTFIAVAAVIA
                     VIGYRVFKGEERAPPAMTNFAGIPAGAKVLSTAIGDGRLVLTVELNGGIELLSFDLNT
                     LRPVGRVRLAP"
     gene            729..1730
                     /locus_tag="VFL49_12040"
     CDS             729..1730
                     /locus_tag="VFL49_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7912755.1"
                     /translation="MTDVATETVTIGADEAGLRLDRVLAARLAHLSRSRLKALILAGQ
                     VALAGRTIRDPAATVKSGDSVKVVLPPPEPAAPQGENIPLNIVYEDDAIIVIDKPKDL
                     VVHPAAGHASGTLVNALIAHCGDSLSGIGGVKRPGIVHRLDKDTTGLMVVAKTDKAHR
                     ALARQFAEKSEGPLRRGYLTFVWGAPAKPKGTIDAPLGRHPQARDKQAVRPSGRAAVT
                     HWQVLERFNPSAAKTPVASLLACTLETGRTHQIRVHLAHWGHPILGDTTYGTGFKTKA
                     SLLGPQARDALLALGRQALHAYLLAIEHPETGDILEFQSELPGDLRRLQGALRTESP"
     gene            1975..2883
                     /gene="rpoH"
                     /locus_tag="VFL49_12045"
     CDS             1975..2883
                     /gene="rpoH"
                     /locus_tag="VFL49_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HET7912756.1"
                     /translation="MARTAALPALKAESGLSHYLDEIRRFPMLEPQEEYMLARSWREH
                     GDREAAHKLVTSHLRLVAKIAMGYRGYGLPISEVISEGNVGLMQAVKRFEPEKGFRLA
                     TYAMWWIKAAIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLRPDQVQ
                     LIAKRLGVTEQDVVDMNRRLGGDASLNAPIRDDGESGEWQDWLVDDAPSQERILAESE
                     ESDNRHKALVSALDVLNDRERRIFEARRLADDPITLEELAAEFNVSRERVRQIEVRAF
                     EKVQKAVKSRMAAMEKPAETPAVTAY"
BASE COUNT          597 a          948 c          922 g          500 t
ORIGIN      
        1 aagaatccga tcggcgtcaa gggatcgggc gagtgcggcg tcacgggatc cattccggcc
       61 gtgctgaatg cggtcaatga cgcgctcgca cgcgccggtg cctcgactgc aatcggtctg
      121 ccggtgacgc cggaaaagat ctggcgctgc ctgcgtgacg ggagagccct gaaagccaag
      181 cggcgacgat gaccggcggt tctagtggcg cctggcgcaa gcgtttgaac gtagtgcagg
      241 aattttttgc aatggcgctc cctaggggac tcgaacccct gttttcgccg tgagagggcg
      301 acgtcctggg ccgctagacg aagggagctt gggccctccg aaatagcctc gatcggcggg
      361 catcgcaagc gtcccggctc gatgctcatt aaggcgccag gcgcacccgt ccgacgggcc
      421 tgagcgtatt gaggtcaaaa ctgaggagtt cgatcccccc gttgagttcg acggtgagca
      481 ccaaccggcc gtcgccgatc gcggtcgaaa gcactttggc cccggcaggg attccagcaa
      541 aattagtcat agcgggcggt gctctttctt cgcccttgaa aacacggtag ccgatgaccg
      601 cgatgaccgc ggcaacggcg atgaaggtgg tcatggtcgc aatcatcatc aggcggcgca
      661 ctttggcgac cacgcgtgca gcttccggat cgagaagttt ttcgctatcg cctgacattc
      721 cctgaaccat gacagatgtt gccaccgaaa ccgtcacgat cggcgccgat gaagccggcc
      781 tcagactcga ccgcgtgctt gccgcgcgtc ttgcacatct gtccaggtcg cggctgaagg
      841 cgctgatcct cgccggacag gtcgcgctcg ccggacgcac gatccgcgat ccggcggcca
      901 ccgtcaaatc cggggattcg gtaaaggtcg tcctgccgcc gcctgaaccg gccgcgccgc
      961 aaggcgaaaa tattccgctc aacatcgttt acgaggacga cgcgataatc gtgatcgaca
     1021 agcccaagga tctggtcgtt catccggccg ccggccacgc gagcggcacg ctggtcaatg
     1081 cgctgattgc ccattgtggc gacagcctgt caggcatcgg cggggtcaag cggccgggca
     1141 tcgttcatcg cctcgacaag gacacgaccg gcctcatggt cgtggcgaag accgacaaag
     1201 cgcaccgcgc gctggcgagg caattcgccg aaaaaagcga aggcccgctc cggcgcggct
     1261 acctaacctt cgtgtggggc gcacccgcaa agccgaaggg cacgatcgac gccccgcttg
     1321 gccgtcaccc gcaggcacgc gacaaacagg cagttcgccc cagcgggcgt gcggccgtca
     1381 cccattggca ggtactcgaa cggttcaatc cttccgctgc caagacccct gtcgccagcc
     1441 tgctcgcctg caccctcgag accggacgca cccatcagat ccgcgtacat ctggcccatt
     1501 gggggcatcc aattctcggc gacaccacct atggcaccgg attcaagacc aaagcgagcc
     1561 ttctcggccc gcaggcgcgc gacgccctgt tggcccttgg caggcaagct ctccatgcct
     1621 atctactcgc cattgagcac cctgaaaccg gagacatcct ggagttccaa tcggaactgc
     1681 cgggcgattt gcgtcgttta caaggtgccc tgcgcacgga aagcccgtga actgggctac
     1741 gagctgcgca aataaaccag ccgcaccaaa ggcttggtac ttcgctgcga cgtgttgttg
     1801 cgtcacagtg acgtgacggg cgcaagccta tctttcgccc ggtcgagccg gtatgctgca
     1861 gttgcgatcg agggacttcg cgttcgcaac acccaaatgt gcgccgtcgg ctcggggagc
     1921 ggcggcgaca acccgcctgc cgatcaaccg ggggcatgta aggagggcgc tgtcatggcc
     1981 cgcaccgccg cattgccggc actcaaggcc gaatccggcc tttcacacta tctcgacgag
     2041 atccgccggt tccccatgct ggaaccgcag gaagaataca tgctggcacg cagctggcgt
     2101 gagcacggcg atcgcgaagc cgcgcacaag ctcgtcacca gccaccttcg tctcgttgcc
     2161 aagatcgcca tgggctatcg cggctacggc ctgccgattt ccgaggtgat ctcggaaggc
     2221 aatgtcgggc tgatgcaggc ggtcaagcgc ttcgaaccgg agaaaggctt ccggctcgcc
     2281 acctatgcga tgtggtggat caaggcggcg atccaggaat acatcctgcg ctcgtggtcg
     2341 ctggtgaaga tgggcacgac cgcgaaccag aagaagctct tcttcaacct tcgcaaagcg
     2401 aagagcaaga tttcggcgct cgacgaaggc gatttgcggc ccgaccaggt ccagctcatt
     2461 gcaaagcgcc tcggagtcac tgaacaggac gtcgtcgaca tgaaccgtcg ccttggcggc
     2521 gatgcatcgc ttaatgcacc aatccgcgac gacggcgaga gcggcgaatg gcaggactgg
     2581 ctggtcgatg atgcaccgag ccaggaacgc atccttgccg agagcgagga gagcgacaac
     2641 cgccacaagg ccctcgtgtc cgcgctcgac gtgctcaacg accgcgagcg gcgcatcttc
     2701 gaagcacgcc ggcttgccga cgatccgatc acgctcgagg agctggctgc cgagttcaat
     2761 gtctcgcgcg aacgcgtgcg gcagatcgag gtgcgcgctt ttgagaaagt tcagaaggcg
     2821 gtaaagagcc ggatggccgc gatggagaag ccggcggaaa ctccggcggt caccgcgtac
     2881 tgaattctac aaaaatggcc gggtccctgc ccggccattc gcttagcggc cacggcaacg
     2941 atctactgcc cccaggcacg gaaagct
//
LOCUS       DASSRL010000591         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1642814, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000591 DASSRL010000000
VERSION     DASSRL010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642814"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFL49_12050"
     CDS             complement(<1..868)
                     /locus_tag="VFL49_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET7912757.1"
                     /translation="MTTVPEIPLPSSIRSRTIDNINGLRMHVLEAGFETSGRPCVLLL
                     HGFPELAYSWRKVMPVLAEAGYHVVAPDQRGYGRTTGWDADYDGDLTAFRLTNLVRDA
                     LGLVSAFGYRSVDAVIGHDFGSSVAAWCALIRPDVFRSVVMMSAPFGGPPSLPFNTVR
                     EPARRADDPIHRDLAALSRPRKHYHWYYSTREANADMHRAPQGVHDFMRAYYHHKSAD
                     WKANKPYPLKSWTADEIAKLPTYYVMDLAKDMAATVAEEMPSAAEIAANKWLPDSELA
                     FYSAEYERNGFQG"
     gene            complement(919..2064)
                     /locus_tag="VFL49_12055"
     CDS             complement(919..2064)
                     /locus_tag="VFL49_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017959319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HET7912758.1"
                     /translation="MPHHNTVFGDILKLVPWRRFERLVDDHDADARVRQLTTKGQFVA
                     LLYGQLSGASSLREIVTGLSSHAARLYHLGADPVRRSTFSDANSQRPVAVFAGLLEVL
                     MRQAHRGLRRKLAETTYLIDATCVRLNERSAGWARFSAGVCGAKVHVIYDADADRPIY
                     AAVSAANVNDITAAHRMPVEPGATYVFDLGYYDYAWWAELDAAGCRIVTRFKSNTPLE
                     VVKELAVSPGSNILSDRIGFLPARQAKSRRNPMQDAVREVRITTDTGKTLRILSNDLD
                     ASAQEIADLYRRRWAIELFFRWVKQTLKITRFIGTSENAVRIQIAVALIAFLLLRLAQ
                     AAQKAIPSPIVFSRLIRTNLMHRRSIDNLLEPPPILQDQRQMSLSLC"
BASE COUNT          435 a          683 c          760 g          437 t
ORIGIN      
        1 cgccctggaa tccattgcgt tcgtactcgg cgctgtagaa agcgagctcg ctgtccggca
       61 gccatttgtt ggcggcgatt tccgcggcgg acggcatttc ctcggcgacg gtggcggcca
      121 tgtccttggc aaggtccatc acgtaatagg tcggcagttt cgcgatttcg tcggccgtcc
      181 acgatttcag cgggtagggc ttgttggcct tccagtcggc gctcttgtgg tggtagtagg
      241 cgcgcatgaa gtcatgcacg ccctgcggcg cgcggtgcat gtcggcattc gcctcgcgcg
      301 tcgagtagta ccaatggtaa tgttttcgcg gacgcgacag cgccgcgagg tcgcggtgaa
      361 tgggatcatc ggcgcgtctg gcgggttcgc gcaccgtgtt gaagggcagc gacggcgggc
      421 cgccgaacgg cgcgctcatc atgacgaccg agcgaaacac gtcgggccgg atcagcgcgc
      481 accaggccgc gaccgacgag ccgaaatcat gcccgatcac ggcgtcgacg ctgcgatagc
      541 cgaacgccga caccagcccg agcgcatcgc gcaccagatt ggtcagccgg aacgcggtga
      601 gatcgccgtc atagtccgcg tcccatccgg tggtgcggcc atagccacgc tggtccggcg
      661 cgacgacgtg gtagccggcc tccgcgagaa cgggcatcac cttgcgccag ctataggcga
      721 gttcggggaa gccgtgcagc agcagcacgc agggccggcc tgaagtctcg aagccggctt
      781 ccagcacgtg catgcgaagg ccgttgatgt tgtcgatggt tcgggagcgg atggaagagg
      841 gaagcggaat ctcggggacg gtggtcattt tttatcctcg tcatggccgg gcttgtccca
      901 cgggtgtccg gttaggggtt agcatagact taggctcatc tgtcgctgat cttggaggat
      961 tggcggcggt tcgagcaagt tgtcgatgga gcggcggtgc atgaggttgg tgcggatcag
     1021 ccggctaaag acgatggggc ttggaatggc tttctgtgcg gcctgagcga gacgtaacag
     1081 caggaaagcg atgagcgcga cggcaatctg gatgcgtacg gcgttttcgg aggtgccgat
     1141 gaagcgggtg atcttgaggg tctgcttgac ccagcggaag aacagctcga tggcccaccg
     1201 gcggcggtag agatcagcaa tttcctgcgc actcgcatcg aggtcgttcg acaggatgcg
     1261 caacgtcttt ccagtgtcgg tcgtaattcg cacctctcgc acggcatcct gcattggatt
     1321 gcgccggctt ttggcttgcc gcgccggcag aaagccgatg cggtcggaga gtatgttgct
     1381 gccgggagag accgccagtt ccttcaccac ctccagcggg gtgttggatt tgaagcgggt
     1441 gacgatccgg caaccggccg cgtcgagttc cgcccaccag gcgtaatcgt aatagccgag
     1501 atcgaacacg taggtggctc ccggctcgac cggcatccga tgagccgcgg tgatatcgtt
     1561 gacgttggcc gcgctgaccg cggcatagat cggccgatcc gcatcggcat catagattac
     1621 atgcaccttg gcgccgcaga ccccggcgga gaaccgggcc cagccggcac tgcgttcgtt
     1681 cagccgcacg caggtggcat cgatcagata ggtcgtctcg gcgagcttgc ggcgcagccc
     1741 gcgatgggcc tgcctcatca ggacctccaa aaggcctgca aacactgcga cagggcgctg
     1801 cgaattggca tccgagaagg tcgatcggcg caccggatcc gcgcccagat ggtaaagccg
     1861 cgccgcatgg ctcgaaagcc cggtcacgat ctcgcgcagg ctcgaagccc ccgaaagctg
     1921 tccgtaaagc agcgccacaa actgcccctt ggtcgtcagt tgccgcaccc gcgcatccgc
     1981 atcatggtca tccacaagcc tctcaaaccg acgccatggc accaacttca gaatgtcgcc
     2041 gaatactgta ttgtgatgtg gcatggcgct gatctctatt cgtgtccgga acgcttgcag
     2101 acgtccagaa ccgactagaa tcaacgccat gcgccttgtc caacaaattt aaaccggaca
     2161 gccgtgggtc aggcccgggg gtgacaagga cgaggacgcg gataaaaaag cccgcccttc
     2221 ggcgggactc ctttagtctc gcatcattct ttagtcttgc atcaagtccg gctagcggtc
     2281 ctgaccaacg ggttggttcg aagggaacac cggtt
//
LOCUS       DASSRL010000592         4756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_120746, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000592 DASSRL010000000
VERSION     DASSRL010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120746"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1355
                     /locus_tag="VFL49_12060"
     CDS             90..1355
                     /locus_tag="VFL49_12060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7912759.1"
                     /translation="MHDMTAKKAAVTAVDKHPSFQKHHRKFPTTAWLRRRAPRNVPLF
                     AFEYGDTGAGNDAGIARNWAALDAVEIAPRYGVMPTLPPVDVKLFGRRYSAPIGVAPM
                     GGPSLVWPGADLLMAKAAQRVRVPYTLSVAGGATIEQAAEAAPDVFWLQLYRFWHDDH
                     KIGFDLVRRAQAVDAKVLVLTLDVPVRTTRSRETYAGLAHEFRPNVRMIYEMLVRPRW
                     MLALLRNGYPRFATISDYARPGAGTNEIIAYARARMGGAFSWEEVKRYRDAWKGPMAV
                     KGVLHPDDAEIAVGLGIDGIWVSNHGGRQIEALATSIDVLPAIAAAVGKKATILLDSG
                     IRSGQDVMRALALGADAAFAGKSFLWAVGALGDGGPDYLIDLYIDELRASLGQIGARS
                     IAEARDASIRHPGAFEFPVKRATKAARKR"
     gene            1512..2696
                     /locus_tag="VFL49_12065"
     CDS             1512..2696
                     /locus_tag="VFL49_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912760.1"
                     /translation="MPDDNLARRNAMVLSVAQALAGGNNTVIVSTASIVGAVLAPDKG
                     LATLPVTAMVFGMWLGTLPLGVLARKFGRRFALQCGSAFGVLSGLISYSAVMNGNFWL
                     LLFGTFCGGLYAAAHQSYRFAAADTASDAYRPKVVSWVLAGGVFAAVLGPQLVIFTKD
                     LLTPHLFAASYLGQSACAVAAACVLQLVKVPPLATPRHGSVRPLGEIVQSSRFIVAVA
                     CGIASYAMMNMLMTSAPLAMVGCGHSVTDAALGIQWHVLAMYAPSFVTGSLIARFGVE
                     RVTGTGLLLIAATAVVGISGLTVAHFWTALVLLGVGWNLSFIGATTMVTQCHRPHERN
                     KVQAFNDFLIFGSMAVSSFSSGQLLENFGWATINEVIFPTIFVTGALLVWLAMRPRPA
                     AV"
     gene            2891..>4756
                     /locus_tag="VFL49_12070"
     CDS             2891..>4756
                     /locus_tag="VFL49_12070"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET7912761.1"
                     /translation="MSTLWLIVGCGVLAIVYGIWAIQSVLSADPGNKRMQEISEAVRE
                     GAQAYLKRQYITIGIAGVVIFVIIGFTLGWLVAVGFAIGAVLSGATGFIGMNVSVRAN
                     VRVAQAATKTLASGLELAFKSGAITGMLVAGLALLGVTLYFGFLTGTMHKAPNDRTVI
                     DAIVALGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNVG
                     DNVGDCAGMAADLFETYAVTTVATMVLASIFFAGTPMLLTMMIYPLAIGGACIVTSII
                     GTFFVRLGRNQSIMGALYKGFIASALLSLVAIWFVTDSLIGFGRIGGANFTGQALYIC
                     AIVGLVVTGLIIWITEYYTGTNFRPVKSIASASVTGHGTNIIQGLAVSLEATALPALV
                     IIAGILVAYAQAGLFGIAIAVTSMLALAGMVVALDAFGPVTDNAGGIAEMAGLPKNVR
                     KATDALDAVGNTTKAITKGYAIGSAGLGALVLFAAYNQDLQFFTRTAAQHPYFQGIQL
                     DFSLNNPYVVVGLLFGGLLPYLFGALGMTAVGRAAGAIVEEVRRQFREKPGIMKGKDK
                     PDYGRAVDMLTKAAIKEMIVPSLLPVLSPIVVYFVINAIAGQAAAFSAVGAMLMGV"
BASE COUNT          787 a         1452 c         1508 g         1009 t
ORIGIN      
        1 caagcgcgcc tctttcagtc atttctgaat tggcgggacg gcgtgggggc gtggggctat
       61 aatgtggctg taagggacgg aaagttacga tgcatgacat gacggcgaag aaggcggcgg
      121 tcactgcggt cgacaagcac ccgagctttc agaaacacca caggaaattt ccgaccacgg
      181 cgtggctgcg gcgtcgcgct ccgcgcaacg tgccgctttt tgcgttcgaa tacggcgaca
      241 ctggtgccgg caacgacgcg ggcatcgccc gaaactgggc ggctttggac gcggtcgaga
      301 tcgctccgcg ttacggcgta atgccgacgc tgcctccggt cgatgtgaag ttgtttggcc
      361 gccgctacag cgcgccgatc ggtgtcgcgc cgatgggcgg cccctcgctc gtctggccgg
      421 gagccgatct tctgatggca aaggccgctc agcgcgtgcg cgtgccatat acgctgagcg
      481 ttgcgggcgg tgccaccatc gagcaggcgg cggaagcggc gcccgatgtg ttctggctcc
      541 agctctacag gttctggcac gacgatcaca agatcggttt cgacctggtc cgccgtgcgc
      601 aagcggtcga tgccaaggtg cttgttctta cgctggacgt tcccgtgcga acgacgcgct
      661 cgcgcgagac ctatgcaggg cttgcccatg agtttcgccc caacgtgcga atgatctacg
      721 agatgctggt acggccgcgg tggatgctcg ctttgctgcg taacggctat ccccgtttcg
      781 ctaccatcag cgactatgcc cggccgggcg caggcactaa cgaaatcatt gcctacgcgc
      841 gcgcccgcat gggcggtgcg ttttcctggg aagaggtcaa gcgctatcgc gacgcatgga
      901 aagggccaat ggccgtcaag ggcgtattgc accccgatga tgcagagatc gcagtcggac
      961 ttggcattga cgggatttgg gtgtcgaacc acgggggccg gcagatcgaa gccctcgcaa
     1021 cctcgatcga cgtgcttccg gccattgccg cagcggtcgg caagaaggcc accattcttc
     1081 tcgacagtgg aatccgcagc ggtcaggacg tgatgcgcgc gctggcgctt ggcgccgacg
     1141 cggcctttgc gggcaaatcg ttcctctggg cggtcggcgc gctcggcgac gggggcccgg
     1201 attacctgat cgatctctat atcgacgagc tgcgagcgtc tctgggccag atcggagcac
     1261 ggtcgatcgc ggaagcgcgg gatgcaagta tccgtcatcc tggcgctttt gagtttccgg
     1321 tcaaacgggc cacgaaagcg gcacgcaagc gttagaccgg gatcaatcag ccggcgaaat
     1381 caaggtttca ataaaattca tccctgatct gcggcgccgg cagtggcgat tcactcccgc
     1441 caccgataca aagggccgct ttttgcctgg gaacccttat gtcagccgtc gccgaaccgc
     1501 ttgagcacat agtgccggac gacaacttgg cgcggcgaaa tgcgatggtg ctgtcggtcg
     1561 cgcaagcgct cgccggcggc aacaacacag tcatcgtgag caccgcaagc attgttggcg
     1621 ccgtgctggc gcctgacaaa ggcttggcga ccttgccggt gaccgcgatg gtgttcggca
     1681 tgtggctcgg cacgttgccg ctcggcgtgc tggcgcgcaa atttggccgc cgtttcgccc
     1741 tgcaatgcgg atcggccttt ggcgttctct ccggcctgat ctcctattcg gcggtgatga
     1801 acggcaattt ctggctgctg ctcttcggca cgttttgcgg gggtctctat gcggccgcgc
     1861 atcaatccta ccgctttgca gctgccgaca ctgcaagcga tgcctatcgg ccgaaggtcg
     1921 tatcatgggt tctcgcgggt ggcgtattcg cggccgtcct cgggccgcaa ctcgtgatct
     1981 tcaccaagga tttgctgacg ccgcatctct ttgcggcaag ctacctcggt caatcggcct
     2041 gtgccgtggc agcggcgtgt gtgcttcaac tggtcaaagt gccaccgctt gcgactcctc
     2101 gccacggaag cgtgcgaccg cttggcgaga tcgtgcagag ttcacgtttc atcgttgcgg
     2161 tcgcgtgcgg catcgcaagt tacgccatga tgaatatgct gatgacgtcg gccccgctgg
     2221 cgatggttgg ctgcggccat tcggtaaccg atgctgcgct cggaattcag tggcatgttc
     2281 tggcgatgta tgcgccaagc tttgtcaccg gctcgctgat cgcgcgcttc ggggtcgagc
     2341 gcgtcaccgg cacgggactg ttgctgatcg ccgcaacggc cgttgtcggc atttcggggc
     2401 ttacggtcgc tcacttctgg accgctcttg tgcttctcgg cgtcggctgg aatttatctt
     2461 tcatcggcgc gacgacaatg gtgacgcaat gccaccggcc gcacgaacgg aacaaagttc
     2521 aggctttcaa tgatttcctg atcttcggca gcatggcggt aagttccttc tcttcggggc
     2581 agttacttga gaatttcggg tgggcaacga tcaatgaagt gattttcccg acgatcttcg
     2641 tgaccggcgc tcttttggtc tggctcgcca tgcggccgcg gcctgctgcg gtctaggcgg
     2701 cagaaaaagg gcgcaccggg ataagggtcc gcccaaattc cgcgttgcgc ttaccacaag
     2761 tttttggcta tatccgcgcg cttaccggtc ggggtgaagc gcccttacaa gcggccatcc
     2821 tcgacgctcc tggcgccggc gggagcgttc gtcccgccag cacctatcaa aatcgaggaa
     2881 agtggccacc atgtctacgt tgtggttgat cgtcggctgt ggggtgctcg cgatcgtcta
     2941 cggaatctgg gcgatccaat cggttctgag cgccgacccc ggcaacaaaa gaatgcagga
     3001 aatttccgag gccgtgcgcg agggcgcgca ggcctatttg aagcgccaat acatcaccat
     3061 cggcatcgcg ggagtggtga tcttcgtcat cataggattc accttgggct ggctggtcgc
     3121 tgtgggcttt gcgatcggtg ccgtcctgtc gggggcgacc ggctttatcg gcatgaacgt
     3181 gtcggttcgc gccaatgtgc gggtggcgca agctgcgaca aagacgttgg ccagcggcct
     3241 ggaactcgcc ttcaagtcgg gtgccatcac gggcatgctg gtcgcgggtc tcgcgctgct
     3301 cggcgttacg ctctatttcg gcttcctcac cggcaccatg cacaaggcgc ccaatgaccg
     3361 cacggtcatc gacgccatcg ttgcgctcgg gtttggcgcc tcgctgatct cgatcttcgc
     3421 gcgtctcggc ggcggcatct tcaccaaagg ggccgacgtc ggcggcgacc tcgtcggcaa
     3481 ggtggaagct ggcatcccgg aagacgaccc gcgcaatccg gcgaccattg ccgataacgt
     3541 cggcgacaat gtcggcgatt gcgccggcat ggccgccgac ctcttcgaaa cctacgcggt
     3601 cacgaccgtc gccaccatgg tgctggcctc gatcttcttt gccggcacgc cgatgctgct
     3661 gaccatgatg atctatccgc tcgccatcgg cggcgcgtgc atcgtcactt cgatcatcgg
     3721 cacgttcttt gtgcggcttg gccgcaacca gtcgatcatg ggcgctcttt acaagggctt
     3781 tatcgcctcg gcactgctgt cgctggttgc gatctggttt gtcaccgact cgctgatcgg
     3841 ttttggccgc atcgggggcg ctaatttcac gggtcaggcg ctctatatct gcgccatcgt
     3901 cggactggtg gtgaccggcc tgatcatctg gatcaccgag tactatacgg gcaccaactt
     3961 ccgcccggtg aagtcgattg cctcggcatc cgtcaccggc cacggcacca acatcatcca
     4021 ggggctcgct gtttcgctgg aagccaccgc attgcccgcg ttggtaatta tcgccggcat
     4081 tctggtggcc tatgcgcagg ccgggctgtt tggcatcgcc attgcggtca cctcgatgct
     4141 cgcgcttgcc ggcatggtgg tcgcacttga tgcgtttggc ccggtcaccg acaatgccgg
     4201 cggcatcgcc gagatggctg gtctgccgaa aaacgtgcgc aaggcgactg acgcgctcga
     4261 tgcggtcggc aacaccacca aggcgatcac caaaggctac gccatcggct cggccggtct
     4321 tggcgcgctg gtgctgtttg ctgcctataa tcaggacctg cagttcttca cgcgaactgc
     4381 agcgcagcat ccgtactttc agggtatcca gctcgatttc tcgcttaaca atccttacgt
     4441 cgtcgttggt ctgctgttcg gcggcctgct gccgtatctg ttcggcgcgc tcggcatgac
     4501 cgccgtcggc cgcgccgccg gcgcgatcgt cgaggaagtg cgccggcagt tccgtgagaa
     4561 gcccggcatc atgaagggca aggacaagcc ggactatggg cgtgcagtcg acatgctcac
     4621 caaggccgcg atcaaggaaa tgatcgtgcc gtcgcttctg ccggtgctct cgccgatcgt
     4681 cgtctacttc gtcatcaacg ccatcgccgg ccaggccgcg gccttctccg cggtcggcgc
     4741 catgctgatg ggcgtg
//
LOCUS       DASSRL010000593         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1013333, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000593 DASSRL010000000
VERSION     DASSRL010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013333"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFL49_12075"
     CDS             complement(<1..253)
                     /locus_tag="VFL49_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912762.1"
                     /translation="MVEKRHSTLWPGVVVLAIATGYLIALLGAEKQNLIIMLLAAGIV
                     AVLAGAWLGLLENVSTSFVEHEDALGVCAIGAACAVAAVF"
     gene            complement(258..1178)
                     /locus_tag="VFL49_12080"
     CDS             complement(258..1178)
                     /locus_tag="VFL49_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912763.1"
                     /translation="MDAGLLAQYVMNGLMLGMMYALVAVGFTLFFGVLDVIKFSHGDV
                     LTVGAFTALASYVGLRMLGLNSSWLNLVVMLVVSTLAMAFLGMAIARYLVMPLRSAPA
                     LNTLLITLMLGTVLRESVRLFYPQGANPKPFPALLPRASVDLGDFNLRLDNVLMLTAG
                     VLVIVGIQYLLNRTKLGLAIRAVAQDEETARTMGINFTAIVLVTFALGSGLAAFAGVM
                     NGLYYNEINFGIGLYLGVVGFSAAIIGGLGSIYGAILGGFLFAGLQTIGTVMLPFASA
                     YKDVFAFAVVIAIMAWRPTGLIREKISERV"
     gene            complement(1245..2414)
                     /locus_tag="VFL49_12085"
     CDS             complement(1245..2414)
                     /locus_tag="VFL49_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7912764.1"
                     /translation="MSTTPKIAALIAAATLVVAAGAAEAKDKVKVGFIGPLTGGVSVN
                     GLGGRNSADLAVRLRNADPKSKYEYEMVVLDDECKPNVAVQVATKMAADKDIIAGATH
                     YCSATAIATVDVYHKFGFPVIVWGAVLPDITYRNKYAEVHRVNGTMINQNDANAELIS
                     KLGYKTVAVIHDTTDYGKGHNEYFSKALARIGQAKIVGTFGVTADQQDFTAELTKIKA
                     LNPDVIYFGGLTPIGVRIRSQMDKLGIKAVFDGTSGIVSDAYIQGLGPLAEGTLAFRE
                     GAPTEKLPGGKFFMEKYSAQKYDNPPEAYGAFAYAAMDMILDAIEKVGPDRKKVIADL
                     SNVKDRDSIVGKITFDDHGQNTVPIITKYVVQDGKFVEWADSEYASGKRKLPGQK"
     gene            complement(2572..>3708)
                     /gene="lanM"
                     /locus_tag="VFL49_12090"
     CDS             complement(2572..>3708)
                     /gene="lanM"
                     /locus_tag="VFL49_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0018081 - peptide cross-linking via
                     lanthionine or 3-methyl-lanthionine [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="HET7912765.1"
                     /translation="NSAAWLGLDWLGDAEVFQLVTLGPDLYNGTAGLSLFLSAHAAVA
                     GQSASADLALAGVARLRKQLKSRNAARIARSLGVGGAIGLGSAVYALTVMSKNLRDPG
                     LRDDAHRAAELMTDELVAADKRLDVIAGSAGAILCLLRLYRDGQSEGALRRAIKCGEH
                     LLAQQRMGETGRRSWVGQGMGPRPLNGMSHGAAGFAYALASLSAATGREDFAAAAQEC
                     VDFENSSFSAQRGNWPDLRVEGTEAWPCRWCHGAPGIGLGRIGMARLPGTDGAVLKAD
                     VQGAVEGTRTAWPSPLDTLCCGTLGSIEFICEAGDFFGRDDLRDLAKREMASVIRAAA
                     DTGDYRWNSGERQFNLGLFRGMAGVGYTALRQVDPSLPNVLIFE"
BASE COUNT          764 a         1177 c         1091 g          676 t
ORIGIN      
        1 gaaaaactgc ggccaccgcg cacgctgcac caatggcgca aacgcccaag gcatcctcgt
       61 gctccacgaa tgaggtgctg acgttctcga gcaggccgag ccaggcaccg gcaagcaccg
      121 ccacgatccc ggccgcgagc agcatgatga tcagattctg tttttcggcg ccgagcagcg
      181 caatgagata accggtcgcg atcgcgagaa cgacgacacc cggccacagc gtgctatgcc
      241 gcttttccac catcggccta gacccgctcg ctgatttttt cgcggatcag accggtcggc
      301 cgccaggcca tgatggcaat gacgacggcg aacgcgaaga catctttata ggcgctggcg
      361 aacggaagca tcaccgtgcc gatcgtctgc aggccggcga acaggaatcc acccagaatc
      421 gcaccgtaaa tgctgccgag accgccgata atggcggcag aaaagccgac gacgccgagg
      481 tagaggccga tcccgaaatt gatctcgttg taatagaggc cgttcatgac gcccgcgaag
      541 gcggccaggc ccgaaccgag ggcgaaagtc acgagaacga tcgcggtgaa gttgatgccc
      601 attgtccgcg ctgtctcctc gtcctgcgcc accgcacgga tggcgagacc gagctttgtg
      661 cggttgagca gatactgaat gccgacaatc accaggacac ccgcggtcag catcagcaca
      721 ttgtcgagcc gcagattgaa atcgccgaga tcaaccgatg cccgcgggag caaggccgga
      781 aacggtttcg gattggcgcc ctgcggatag aaaagccgaa cggattcccg cagcaccgtc
      841 cccagcatca gggtgatcag aagcgtgttg agcgccggcg ccgagcgcag cggcatcacg
      901 agatagcggg ctatcgccat tcccaggaac gccatagcca gcgtcgacac caccagcatg
      961 acgacaaggt tgagccagct ggaattcagc cccagcatgc gcagcccgac atagctcgcg
     1021 agcgctgtga aagcccccac ggtcagcaca tcaccgtgtg aaaacttgat gacatcgagc
     1081 acaccgaaga aaagtgtgaa cccgaccgcc accagcgcat acatcatgcc gagcatcagc
     1141 ccattcatga cgtactgagc gagcaggccc gcgtccatgt ccggcgatct tgtgaagcgt
     1201 taagaaaaac ggggtcgcgt ccgccgcggc cccgcttgcg tcaactattt ctgccccggc
     1261 agcttgcgct tgccgctggc atactcgctg tcggcccact cgacgaattt gccgtcctgg
     1321 acgacatatt tggtgatgat cggcaccgta ttctgcccgt gatcgtcgaa cgtgatcttt
     1381 ccgacgatcg agtcccggtc cttcacgttc gacaggtccg cgatgacctt cttgcgatcc
     1441 ggcccgactt tctcgatcgc atcgaggatc atgtccatcg ccgcataggc gaatgcacca
     1501 taggcctccg gcggattgtc gtatttctgc gcgctgtact tttccatgaa gaacttgccg
     1561 cccggaagct tctcggtcgg cgcaccctcc cggaaggcca gcgtgccttc ggccagcggg
     1621 cccaggccct ggatataggc gtcggagacg ataccggagg taccgtcaaa caccgccttg
     1681 atgccgagct tgtccatctg cgagcggatg cggacgccga tcggcgtcag gccgccgaaa
     1741 tagataacgt caggattgag cgccttgatc ttggtaagct cggcggtgaa gtcctgctga
     1801 tcggcggtga caccgaacgt gccgacgatt ttggcctggc cgatcctggc gagcgccttg
     1861 ctgaaatact cgttgtgccc cttgccgtag tcggtcgtat catgaatgac agccacggtc
     1921 ttgtagccga gcttggagat caattcggcg ttggcgtcat tctggttgat catcgtgccg
     1981 ttgacgcgat gaacttcggc gtacttgttg cggtaggtga tgtcgggaag cacggcgccc
     2041 cacacgatga ccgggaaacc gaacttgtgg tagacgtcaa cggtcgcgat cgccgttgcg
     2101 gaacaataat gtgttgcacc ggcgataatg tccttgtcgg ccgccatctt ggtggcaacc
     2161 tgcaccgcca cgttcggctt gcactcgtcg tcaagcacga ccatttcgta ttcgtatttt
     2221 gatttcgggt cggcgttgcg caggcggacc gccaggtcgg cggagttgcg cccacccagt
     2281 ccattcacgg atacgccgcc ggtgagtggg ccgatgaagc cgactttcac cttgtccttt
     2341 gcctcggcag ctccagccgc gacgacaaga gtggcggccg caatcagtgc ggcaattttc
     2401 ggagttgtac tcataagtcc tcccttacca agtatcgaaa ttggacgtct tttcgttggc
     2461 gccatagtcg cgcggcaaac gaaccaccgc aagagccgcg tcagaagctg gtctgatgcg
     2521 acatttcgga gagcctggga attttcgccg gctaagaccg gctatctgtc gttactcgaa
     2581 aatcagcacg ttgggcagcg aaggatcgac ctgccgcaag gcggtgtaac cgacccccgc
     2641 catgccacgg aagaggccca ggttgaattg ccgctccccg ctattccaac ggtagtcgcc
     2701 tgtatccgcc gcggcccgta tcacgctggc catttcgcgt ttggcgagat cgcggagatc
     2761 gtcacgtcca aaaaagtcgc ccgcctcgca aatgaattca atggacccca gcgtcccaca
     2821 acacaacgta tcgagcggtg aaggccatgc ggtgcgcgta ccttcgaccg cgccttgaac
     2881 gtcggccttc aggaccgcgc cgtctgtccc tggcaggcga gccatgccga tcctgccaag
     2941 gccgatgccg ggcgctccat gacaccaccg gcaaggccag gcttccgtgc cttcgacgcg
     3001 caaatcaggc cagttgccac gctgcgcact gaagctggaa ttctcaaagt cgacgcactc
     3061 ctgtgccgca gcggcgaaat cttcccgccc ggtcgcggcc gacagagagg cgagtgcata
     3121 tgcgaatccg gctgcgccat gtgacattcc gttgagcggg cgtggaccca tgccctgccc
     3181 cacccagctg cgccgccccg tctcgcccat ccgctgttgc gcgagcaggt gttcgccgca
     3241 cttgatggcg cgacgaagtg cgccctcgga ttgcccgtca cgataaagac gaagcagaca
     3301 aaggatcgcg cctgcactcc cggcaatcac atcgagacgc ttgtccgcgg cgaccagttc
     3361 atcggtcata agctcggccg cacggtgagc gtcgtcacgc aacccgggat cgcgcaaatt
     3421 tttcgacatc accgtcagcg cgtagacagc tgagccgagt ccgatcgctc cgccaacgcc
     3481 aagagaccgg gcgatccttg ctgcattccg ggacttcagc tgctttcgaa ggcgggcaac
     3541 gccggccagg gccaggtcag ctgaggccga ttgccccgcg acggcagcat gtgctgacag
     3601 gaaaagggaa aggcccgcgg tgccgttgta cagatcgggt cccaacgtta ccagctggaa
     3661 cacctcggca tcgccaagcc agtcgagccc gagccaagcg gcggaatt
//
LOCUS       DASSRL010000594         4813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1642893, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000594 DASSRL010000000
VERSION     DASSRL010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642893"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFL49_12095"
     CDS             <1..429
                     /locus_tag="VFL49_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET7912766.1"
                     /translation="GGVVDLYRGLMRGDDALVVHAYETWGFKRLNRELIDALNIWARF
                     IYGPLLDDRVRSIADGVKPSEYGRKEAFRVHQALKARGPVTVPREFVFMDRAAIGLGA
                     VFLHLAAELNFYRLFNEAIERFKVGDVARRQGAALAEAGL"
     gene            763..1062
                     /locus_tag="VFL49_12100"
     CDS             763..1062
                     /locus_tag="VFL49_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HET7912767.1"
                     /translation="MADHGTVEYATATGNDYQAHESSYERFVHFTLVGILYVVTVLFG
                     LATGGVMGHWFLAAATFLIGLLGVIPGLVSGTTTPSYVSFVICFLIFAFTGLSPH"
     gene            1093..2226
                     /locus_tag="VFL49_12105"
     CDS             1093..2226
                     /locus_tag="VFL49_12105"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET7912768.1"
                     /translation="MRIAVPRETDAGEPRVAATPETIKKLKALGAEVAVARGAGVASG
                     IPDAEFEAAGAQVADAVTKDADIVLKVRRPAPNELGDYKKGALVIAIMDPYGNDAALK
                     QMAGAGVSAFAMELMPRITRAQSMDVLSSQANLAGYRAVIDAAEEYGRALPMMMTAAG
                     TVPAAKVFIMGVGVAGLQAIATARRLGAIVTATDVRPATKEQVESLGAKFLAVEDEEF
                     KNAQTAGGYAKEMSKEYQAKQAALVAEHIKKQDIVITTALIPGRPAPRLISKEMVASM
                     RPGSVIVDLAVERGGNVEGAQAGSIAQVGGVKIVGYLNVPGRLAASASALYAKNLLTF
                     VETLIDKKEKKLAVNWDDELVKSTLLTRDGAVVHPSFQPKTAA"
     gene            2238..2561
                     /locus_tag="VFL49_12110"
     CDS             2238..2561
                     /locus_tag="VFL49_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HET7912769.1"
                     /translation="MENVASAVDPFVFRLSIFVLAVFVGYYVVWSVTPALHTPLMSVT
                     NAISSVIVVGALLAVGVALVTNDQGPLWARALGFVALVFASVNIFGGFLVTQRMLAMY
                     QKKKK"
     gene            2731..4125
                     /locus_tag="VFL49_12115"
     CDS             2731..4125
                     /locus_tag="VFL49_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7912770.1"
                     /translation="MSPNLVALLYLVAGVLFILALRGLSSPDSSRRGNLFGMIGMTIA
                     VLTTLGSHPPADAIAWGLVIGGIAVGGGVGAVIARRVPMTSMPELVAAFHSLVGMAAV
                     LVAAGAFYAPAAFDIGTEGHIHAQSLIEMSLGVAIGAITFTGSVIAFLKLSGRMSGKP
                     ITLPGRHIINLALAAALIFFIYGFFVSQSAMDFWIIVALSLALGILIIIPIGGADMPV
                     VISMLNSYSGWAAAGIGFTLGNSALIITGALVGSSGAILSYIMCKGMNRSFISVILGG
                     FGGETAGPGAGAEQRPVKLGSAEDAAYILKNSSKVIIVPGYGMAVAQAQHALREMADK
                     LKKEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQADVAFVIGA
                     NDVTNPAAEEDKSSPIYGMPVLQVWKAGTVMFIKRSLASGYAGIDNPLFYRDNTMMLL
                     GDAKKMTESIVKAM"
     gene            4313..>4813
                     /locus_tag="VFL49_12120"
     CDS             4313..>4813
                     /locus_tag="VFL49_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HET7912771.1"
                     /translation="MIDDRSALGIGEVVPFGRRPIPPRACVVENKTHVRTFLAEMFDE
                     LGFIAREALTLDVRGVLLDFRPDLIVLGPLNGGPDVRSVMQTLRAQGYGGKVMLFGGR
                     SSAALIRNYEFGEAMGLAMLPPLGTPFRDRDLHANLDGFLPVRPAPPLPVDVDEVLHN
                     GWLELWY"
BASE COUNT          874 a         1440 c         1535 g          964 t
ORIGIN      
        1 ggcggcgtgg tcgatctcta tcgcggcctc atgcgtggcg acgatgcgct ggtcgttcat
       61 gcctacgaga cgtggggatt caagcggctt aaccgcgagc tgatcgacgc gctcaatatc
      121 tgggcgcgtt tcatctatgg cccgctgctc gacgaccggg tccgcagcat tgccgacggg
      181 gtgaaaccgt ccgaatacgg acgcaaggag gccttccgcg tgcaccaggc gctcaaggcc
      241 cgggggccgg tgactgtgcc gcgcgagttc gtcttcatgg accgcgcggc aatcggcctc
      301 ggcgcggtgt tcctgcatct ggcggcggaa ctcaatttct atcgcctgtt caatgaggcg
      361 atcgagcgct tcaaggtcgg ggatgtggcc aggcggcagg gcgcggcgct cgccgaggct
      421 gggctctagc gtgtgactct ccacgtcatg gccggacttg tcactgcaag tcgggtttac
      481 ccgacttgca gcaggtattt ttgcggaagt cgggcaagcc cgacttccga tgccatccac
      541 gttttacgat gtggccagac ccaaagacgt ggatgcccgg cacaaggcct gtgctcgggc
      601 cggccgaagg ccggacccga gtgccgggca tgacaaatgt gagaccttac cgagctggct
      661 ttcgcggggg tgagcgggga aaatccgccc gttgccctca atggacaatt gcgctaaccc
      721 ctttgcgccc gccacttttg gaccgactcg gagagatttc caatggccga ccacggcacc
      781 gttgaatacg ccaccgcaac aggcaacgat tatcaagccc acgaaagttc gtatgaacgc
      841 ttcgtgcact tcacgctggt gggcattctc tacgtggtta cggtcctgtt cggcctggcg
      901 accggcggcg taatgggtca ctggtttctt gcggcggcga cttttctcat cggcttgctt
      961 ggtgtgatcc ccgggttggt cagcgggacg acaacgccga gctatgtttc gtttgtcatt
     1021 tgtttcctga tcttcgcctt taccggactt tcgcctcact gacagaattc acgggcgggc
     1081 ggggaggcgc gaatgcgaat tgccgtaccg cgtgaaaccg acgccggcga gccgcgcgtc
     1141 gcggcaaccc cggaaacgat caagaagctc aaggcactcg gcgccgaggt cgcagtcgcg
     1201 cgcggggccg gcgtggcgtc cggcattccg gacgccgaat tcgaagcggc cggcgcgcag
     1261 gttgcagatg cggtcaccaa ggacgccgac atcgttctca aggtacgtcg gcccgcgccg
     1321 aacgaactgg gcgactataa aaaaggcgcc ctggtgatcg ccatcatgga cccgtacggg
     1381 aacgacgctg cgctcaagca gatggcgggc gcgggtgttt ccgctttcgc catggaactg
     1441 atgccgcgca tcacgcgtgc gcagtcgatg gacgtgctgt cgagccaggc caatctcgcc
     1501 ggatatcgcg cggtgatcga tgcggcggaa gaatatggtc gcgcattgcc gatgatgatg
     1561 acggcggccg gcacggtccc cgcggcgaaa gtgttcatca tgggcgtcgg cgtggcggga
     1621 ctacaggcga ttgcgaccgc gcggcgcctg ggcgcgattg tgacggcaac cgacgtgcgt
     1681 cctgcgacca aggaacaggt ggaaagtctc ggcgccaaat ttctcgctgt cgaggatgag
     1741 gaattcaaga acgcccaaac ggcgggcggg tacgcgaagg aaatgtcgaa ggagtatcag
     1801 gccaagcagg ctgctctggt cgccgagcac atcaagaaac aggacatcgt catcaccacc
     1861 gcgctgatcc ctggacggcc cgcaccgcgt ctcatttcga aggaaatggt ggcgtcgatg
     1921 cggccaggct cggtgatcgt tgatcttgcg gtcgagcggg gcggcaacgt cgaaggcgcg
     1981 caggcgggca gcatcgcgca ggtcggtggc gtgaagatcg tcggttatct caacgtgccg
     2041 ggacggctgg cggcttcggc gtcggccctc tacgccaaga acctgctcac ctttgtcgaa
     2101 actctcatcg acaagaaaga gaagaaactt gcagtcaatt gggacgacga actggtcaag
     2161 tcaacgcttc tgactcgcga cggagcagtc gtgcatccga gctttcagcc gaagacggcg
     2221 gcctgaggag agccgatatg gaaaacgtgg caagcgcagt cgatcccttt gtcttccggc
     2281 tttcgatctt tgtgctggcc gtgttcgtcg gctactacgt cgtgtggtcg gtgacgccgg
     2341 cactgcacac gccactgatg tcggtcacca atgccatttc atccgtgatc gtggttggtg
     2401 cgctgctcgc ggtcggcgtc gcgctggtca cgaacgatca gggccccttg tgggcccgcg
     2461 ccctcggctt cgtcgccctg gtctttgcat cggtgaatat cttcggcggc ttcctggtca
     2521 cgcagcgcat gctggcgatg taccagaaga agaagaaatg acgacgacaa tgaatcctat
     2581 tccaaaaatg tcgtcgtcat ggccggactt gttccggcca gccacgtctt ccttgctgtc
     2641 acgccgcaaa ggcgtggatg cccggcaaat aggcgtgcga aagcacgccg ttcttcgaac
     2701 ggctatggcg gggcatgacg gggcgccccc atgagcccga acctcgtcgc gctgctctat
     2761 ctggtcgccg gcgtcctgtt catcctggcg ctccgcggac tgtcgagccc ggactccagc
     2821 cggcgcggca acctgttcgg catgatcggc atgacgatcg cggtgctgac caccttgggc
     2881 tcgcacccgc cggccgatgc gatcgcctgg gggctcgtga tcggcggtat tgccgtcggc
     2941 ggcggcgtcg gcgccgtgat cgcgcggcgc gttccgatga cctcgatgcc cgaacttgtc
     3001 gcggcgttcc actcgctggt cggtatggcg gcggtgctgg tcgcggcggg agccttctac
     3061 gcgcccgctg ccttcgatat cggcaccgag ggtcatatcc acgcgcaaag cctgatcgaa
     3121 atgtcgctcg gggtggcgat tggcgccatc accttcaccg gctcagtcat tgcgttcctt
     3181 aaattgtcag gccgcatgag cggcaagccg atcacgctgc cgggcaggca tatcatcaat
     3241 cttgcgctcg ccgctgccct catcttcttc atctacggtt ttttcgtctc gcaaagcgcg
     3301 atggatttct ggattatcgt tgcgctgtcg ctcgcgctcg gcatcctgat cattatcccg
     3361 atcggcggtg ccgacatgcc ggtcgtgatc tccatgctca actcctattc ggggtgggcg
     3421 gccgccggca tcggtttcac gctcggcaat tcggccttga tcattaccgg cgcgctggtc
     3481 ggctcttcgg gcgcgatcct gtcctacatc atgtgcaaag ggatgaaccg ctctttcatc
     3541 tcggtcatcc ttggcggctt tggcggcgag acggccggcc ccggcgccgg cgccgagcaa
     3601 cgcccggtca agctcggctc ggccgaggat gccgcttaca tcctcaaaaa ttcctcgaaa
     3661 gtcatcatcg tgcccggcta cgggatggca gttgcgcagg cccagcacgc gctgcgcgaa
     3721 atggccgaca agctgaaaaa ggagggcgtc gaggtcaagt acgcaatcca tccggtcgcg
     3781 gggcgcatgc cgggacacat gaacgtgctg ctcgccgaag cgaacgtgcc ttatgacgag
     3841 gtgttcgagc tcgaagacat caattccgaa ttcgcgcagg ccgacgtcgc cttcgtcatt
     3901 ggcgcaaacg acgtcaccaa tccggcagca gaagaagaca aatcatcacc gatctacggt
     3961 atgccggtgc tgcaggtatg gaaggcgggc acggtgatgt tcatcaagcg ctcgctcgcg
     4021 tccggatatg ccggaatcga caatcccttg ttctatcgcg acaacacgat gatgcttctc
     4081 ggtgacgcga agaaaatgac cgagagcatc gtcaaggcaa tgtaagccag cgtacattgt
     4141 cgcgctgaaa ttccatctga ttcaaatttg aaaaactgcg caatcttccc gttaaccgcg
     4201 ggaggaggcg tctgaggatt gtgcggagcg aagtgtagac gaccgcgttg caacaaatat
     4261 ttcatacggc tgcaatttgc cgcatggcca cttctgggga gggaccgccg ccatgatcga
     4321 cgatcgcagc gcattgggaa ttggggaagt cgttcctttc gggcgcaggc caattccgcc
     4381 acgcgcctgt gttgtcgaaa acaagaccca cgtgcgcact tttcttgccg aaatgtttga
     4441 tgagcttggc tttatcgccc gcgaggccct gacgctcgac gtcaggggcg tcctgctgga
     4501 tttccggccg gacctcatcg tgctcgggcc gctcaatggc ggcccggacg tgcggtcggt
     4561 gatgcagacg cttcgggcgc aaggctatgg cggcaaggtc atgctgttcg ggggacggag
     4621 ctcggcagca ctcattcgca actatgaatt cggtgaggcg atggggctgg caatgctgcc
     4681 gccgcttgga acgccgtttc gcgaccgcga tctccacgca aaccttgacg gttttcttcc
     4741 ggtccggccg gcgccgccgt tacctgtcga cgtcgacgaa gtgctgcaca acgggtggct
     4801 ggaactctgg tac
//
LOCUS       DASSRL010000595         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1502921, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000595 DASSRL010000000
VERSION     DASSRL010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502921"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VFL49_12125"
     CDS             <1..954
                     /locus_tag="VFL49_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7912772.1"
                     /translation="WHFQTVHHDIWDLDLPAATLLDVKKDGKTIPAIGVMNKTAMLFL
                     LNRVTGEPIYEVKEVPVPTDTDIPDEAPWPTQPMPVAPPPLARLEFKMSDLTDITPEH
                     RAFCEQLIKDKNIVESTLFQPLRGDSAVASFPGSLGGVDWGGGAFDPKSGLYIVNTND
                     LAGPQQMAKRPDGSWGLKEGYVYFWNPKTRLPCQQPPWGSLYAVDVNKGTIAWRSTLG
                     VSDNLPEGLQNTGRISAGGPIVTAGGLIFIGATDDSRIRAFETKTGKELWTFKLPASN
                     YGTPITYQSRGGKQYVAAIATGGFSGDPVNSDTVISFSLPQ"
     gene            951..1268
                     /locus_tag="VFL49_12130"
     CDS             951..1268
                     /locus_tag="VFL49_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912773.1"
                     /translation="MIKLMSSRYLIALIAVLTATIATPLLAQAPDAPPPGEGLELIER
                     SCINCHDIYMITSKRKTPQEWAATLGVMVDRGADVTPEEMQVIEEYLSQHFAADAANA
                     NAH"
     gene            1360..>2183
                     /locus_tag="VFL49_12135"
     CDS             1360..>2183
                     /locus_tag="VFL49_12135"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7912774.1"
                     /translation="MKLSSRIAAACLLCASIGAASAASDQTPAITRLDPALDAVIAPG
                     TRIEVVATGFKFTEGPMWREGRLWFSDLSDDKVIAVSPAGKVKTLIEHAGGLNPFPPG
                     SFLGSNAMVTDKDGSVLLVQQGGRKIVRLDAKLQPTVLIDKYDGKRLNSPNDLVFAAD
                     GALWFTDPPFGLPQMDKDPAKELAFNGVYRYADGKLTAAIRDLTLPNGLAFSPDGKTL
                     YVANYGPKRFVNAYSVAADGSVSNARTVIEYGPDEKGQGGPDGLKVDRSGNLWTTGP"
BASE COUNT          441 a          692 c          665 g          385 t
ORIGIN      
        1 tggcatttcc agaccgtgca tcacgacatc tgggatctgg atctgcccgc ggcgacgctg
       61 ctcgatgtga agaaggacgg caagacgatc ccggcgatcg gcgtcatgaa caagactgcc
      121 atgctgttcc tgctcaatcg cgtcaccggc gagccgatct atgaagtgaa ggaagtcccg
      181 gtgccgacag acacggacat tccggatgaa gcgccgtggc ccacgcagcc catgcccgtc
      241 gctccgccgc cgctggcgcg cctcgagttc aagatgtccg atctcacgga catcaccccc
      301 gagcatcggg ctttctgcga gcaactcatc aaggacaaga acatcgtcga gagcacgctg
      361 tttcagccgc tgcgcggcga ctccgcggtt gccagctttc ccggaagtct cggcggcgtc
      421 gactggggcg gcggtgcctt cgatccgaag tccggcctct acatcgtgaa caccaatgat
      481 cttgcgggtc cgcagcagat ggcgaagcga cccgacggtt cctggggcct gaaggaaggc
      541 tacgtgtact tctggaatcc gaagactcgc ctcccatgcc agcaacctcc ctggggcagc
      601 ctgtatgcgg tcgatgtgaa caagggcacg atcgcctggc gctcgacact gggcgtgagc
      661 gacaacctgc cggaaggact gcagaacacc ggacgcatca gtgccggcgg acccatcgtc
      721 acggcgggcg gtctgatctt catcggcgcc accgatgaca gccgcatccg cgccttcgaa
      781 acgaagaccg gcaaggagct gtggaccttc aagctgccgg cctccaacta cggaacgccc
      841 atcacctatc aatccagggg cggcaagcag tatgttgccg ccatcgccac cggcggattc
      901 tccggcgacc ccgtcaacag cgataccgtc atctcgtttt ctctgccgca gtgatcaagc
      961 tcatgtcttc gcgctatctg attgctctca tcgccgtact gaccgccacg attgccacgc
     1021 cgctgctggc gcaggcgccg gatgcgccgc cgccgggtga gggactcgag ctgatcgaac
     1081 gcagctgcat caactgccac gacatctaca tgatcaccag caagcgcaag acgccgcagg
     1141 agtgggcagc cactctcggc gtcatggtcg atcgcggtgc tgacgtcacg ccggaagaga
     1201 tgcaggtgat cgaggagtac ctctcgcagc atttcgctgc ggatgctgcg aacgccaacg
     1261 cccattgatt catcacgcga ggacgaactc atgaacagaa gaaagtggct gcaatcgctg
     1321 atcctgctga cgaacgccgt ctgaccccgg ggaggcagca tgaagctcag ttcgcgaatc
     1381 gccgccgcct gtctgctctg cgccagcatc ggcgccgcca gtgcggcatc cgatcagaca
     1441 cccgccatca cacggctcga tcctgcgctc gatgcagtca ttgcgccggg tacgcgcatc
     1501 gaagtcgtcg ccacgggttt caagttcaca gagggcccca tgtggcgcga ggggcggttg
     1561 tggttctcgg acctgagcga tgacaaggtc atcgcagtgt cgcctgccgg caaggtgaaa
     1621 acactcatcg agcatgcagg aggactgaac cccttcccgc ccggctcctt cctcggttcc
     1681 aacgccatgg tcaccgacaa ggacggctcg gtgctgcttg tgcagcaggg tggccgcaag
     1741 atcgtgcggc tcgatgcaaa gctgcagccg acagtgctga tcgacaagta cgatggcaag
     1801 cgactgaaca gtccgaacga tctggtgttc gctgcagacg gcgcgctgtg gttcacagat
     1861 ccgcctttcg gcttgccgca gatggacaag gatcctgcca aagaattggc attcaacggt
     1921 gtgtatcgct atgccgatgg caagctgacg gcggcgatcc gcgatctgac gctgccgaac
     1981 ggcctggcgt tctcgcccga tggcaagacg ctgtatgtcg ccaactatgg accgaagcga
     2041 ttcgtcaacg cgtacagcgt cgccgccgat ggttcggtga gcaatgcacg cacggtgatc
     2101 gaatacggtc cggatgaaaa aggtcagggc gggcccgacg gcctgaaggt ggatcgctca
     2161 ggcaatctgt ggacgacagg tcc
//
LOCUS       DASSRL010000596         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_610465, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000596 DASSRL010000000
VERSION     DASSRL010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610465"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFL49_12140"
     CDS             complement(<1..505)
                     /locus_tag="VFL49_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7912775.1"
                     /translation="MRGLNAFYGAVQVLHSVNLSVEKGEIVGLVGANAAGKSTLMFTL
                     AGLRTSCTGDLLFNKRPIANLPVYERVSLGLVLVPERRRLFPFMTVLENLEIGAYATG
                     ARRVAAQTIEEVFALLPILAERRKQVAGTLSGGEQQMLAIGRALMARPQTLLLDEPTE
                     GLAPIYVK"
     gene            complement(507..1235)
                     /locus_tag="VFL49_12145"
     CDS             complement(507..1235)
                     /locus_tag="VFL49_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912776.1"
                     /translation="MSAILTIDNVSHRFGSLWAVRGVSFSVERGELLGLIGPNGAGKS
                     TLYNLIAGALIPTKGEIIFDGVKVSHWKSHRIARVGIARTFQIPKPYRQLSLVENVML
                     TAFQRENSVSQARELAIETLADVGLSDYIDVPVTALTVGLLKRLEVARALAMRPKLVL
                     FDEIMAGLTPTEVGTMTKFVGGLPARGITVIWVEHVLYAIMKTASRIMVLNRGELIAN
                     GEPAKVARDPGVVKAYLGEEMSLA"
     gene            complement(1232..2251)
                     /locus_tag="VFL49_12150"
     CDS             complement(1232..2251)
                     /locus_tag="VFL49_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912777.1"
                     /translation="MSVGVTHQSSRRHALPFFPRRGTPVSVLEILLFVVCVALTVVGL
                     PQSLQDVAVLTFLWAGLALAWNIAGGYAGLISFGHAAFFGVGAYTSTILFVHVGIIPW
                     IGIWCGAVMAGLFGALLALICARLRGPFLILSTLAAAEVVRIGALNWASLTGGPEGLS
                     IPPVASLTSMVFTSKSAYVVMMLLFLLGVYAVTKFLEGSRYGYYLFSVRDDADAASAA
                     GVNPLVVRTTAMALSAGLTGIGGSLFAQYFLYLDPTFVISPEISFQFALLAAVGGLGT
                     AIGPVLGSFLITPMSELLRSYLGSQAAGLHLVIYSALLIVVMLYFPGGIAGALTRLAD
                     RGDKR"
     gene            complement(2260..>2723)
                     /locus_tag="VFL49_12155"
     CDS             complement(2260..>2723)
                     /locus_tag="VFL49_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912778.1"
                     /translation="LGFAVTILCTLFLQWLLRTTYLGKAIRATVQDGEAAMLMGIPVP
                     RIFLITFAGGSALVGLAACIMMPLFSVFPTVGLNFVLIAFVIVVLGGMGSIEGALLGG
                     ICIGVVQSLSSYYVAPAYGQMFYFILFLLVMVFRPNGLLGQKGAATIGIND"
BASE COUNT          622 a          867 c          809 g          425 t
ORIGIN      
        1 acttcacata aatcggcgcg aggccctcgg tcggctcgtc aagcagcaag gtttgcggcc
       61 gcgccatcag cgcccgcccg atcgcaagca tctgctgttc gccgccactg agcgtcccgg
      121 cgacctgctt gcggcgttcc gccaggatcg gaagaagagc aaagacttcc tcgatcgtct
      181 gggcggcgac ccggcgcgcg ccggtggcgt acgcgccgat ctccagattc tcgagcacgg
      241 tcatgaaggg aaacaggcgc cgccgttcgg gcaccaggac caggccgagc gagacgcgtt
      301 cgtaaacggg cagattcgcg atcgggcgtt tgttgaaaag gaggtcgccg gtgcagctcg
      361 ttcgcaggcc ggccagcgtg aacatcagcg tgctttttcc ggcggcattg gcgccgacga
      421 ggccgacaat ttcacctttc tcgaccgaca ggttgaccga gtgcagaacc tgcaccgcgc
      481 cgtaaaaagc gttgagacct ctcacctcaa gcaaggctca tctcctcgcc caggtaggct
      541 ttcaccacgc ccggatcgcg cgcgaccttc gccggttcac cattggcaat cagctccccg
      601 cgattgagca ccatgatccg gcttgcggtt ttcatgatcg cgtatagcac gtgctcgacc
      661 cagatgaccg tgatgccgcg cgcgggcagc ccgcccacaa acttggtcat cgtgccgact
      721 tcggtaggcg tcaaacccgc cataatctcg tcgaacagaa cgagcttggg gcgcatggcg
      781 agcgcgcggg caacttcaag ccgcttgagc aaaccaaccg tgagggcggt cacgggaacg
      841 tcgatgtagt cgctgaggcc aacatcggcg agcgtctcaa ttgcaagctc cctcgcctga
      901 cttaccgagt tctcccgctg aaaggccgtc aacatgacgt tttcgaccag cgacaactgc
      961 cgatacggct tcggtatctg gaacgtgcgc gcaatgccaa cgcgggcgat gcggtgagat
     1021 ttccagtggc tgaccttcac gccgtcgaat attatctcac ctttcgtggg aatcagcgcg
     1081 ccggcgatca gattgtacag cgtgcttttg ccggcaccgt tcggcccgat caacccgagc
     1141 aattcaccgc gctcgacgga gaagctcacg ccgcgtaccg cccacaacga gccgaaccga
     1201 tggctgacat tgtcgatcgt cagaattgcg ctcaccgctt gtccccccgg tccgcaaggc
     1261 gagtgagggc tccggcaatc ccgcccggaa aatagagcat gacgacgatc agcagcgcgc
     1321 tgtagatgac gaggtgaaga cccgcggctt ggcttcccag ataggagcgc agcagctccg
     1381 acataggcgt gataagaaat gaccccagca ccgggccgat ggccgttccc agtccgccga
     1441 ccgcggccag cagcgcgaac tggaaggaaa tctccggcga aatcacgaaa gtcgggtcga
     1501 gatagaggaa gtactgcgcg aacagcgaac cgccgatccc cgtcagtccc gcgctgagcg
     1561 ccatcgcggt ggtccgcacc accaacggat taacgcccgc ggcactggcg gcatccgcat
     1621 cgtcgcggac cgagaacaaa tagtaaccat aacgcgagcc ttccagaaac tttgttaccg
     1681 cgtagacccc caggagaaac agcagcatca tcaccacgta ggcggatttt gacgtgaaaa
     1741 ccatgctcgt gagcgacgca accggtggga tcgaaagtcc ctcgggtccg cctgtgagag
     1801 aggcccagtt cagcgcaccg atgcggacga cctcggcggc ggcaagcgtg gatagaatca
     1861 ggaaggggcc ccgaaggcgc gcgcagataa gcgcgagcag cgccccgaac agtcctgcca
     1921 tcaccgcgcc gcaccagatg ccgatccatg ggatgattcc gacgtgaaca aacaggattg
     1981 tcgacgtata tgcgccgacg ccaaagaacg ccgcgtggcc gaacgatatc aagccggcgt
     2041 agccgccggc gatgttccag gcgagcgcca atcccgccca cagaaacgtc aagaccgcaa
     2101 catcctgcag actttgcgga agtccaacga ccgtgagtgc gacgcaaaca acgaacagca
     2161 ggatttcaag gaccgaaacc ggcgtgccgc gccgcgggaa aaacggcagc gcatggcggc
     2221 gcgacgattg atgtgtgacg ccaactgaca tgccggccct caatcgttga taccgatcgt
     2281 tgccgcgccc ttttgtccaa gcaatccatt tggccggaaa accatcacca gcaggaagag
     2341 gataaagtag aacatctggc cgtaagcagg cgccacatag tagctgctga gcgactgaac
     2401 gacgccgatg caaatgccgc cgagcagcgc gccctcgatg ctgcccatac cgccgaggac
     2461 cacgatcacg aatgcgatca gcacgaaatt aagtccgacg gtgggaaaca ctgaaaagag
     2521 cggcatcatg atgcaagctg caaggccaac cagcgcggag ccgcccgcaa atgtaatgag
     2581 gaatatccgc ggaaccggga tgcccatcag catcgcggcc tcgccgtcct ggacggtcgc
     2641 gcggatcgcc ttgcccagat aggtggtgcg gagcagccac tgcaaaaaca gcgtgcagag
     2701 gatcgtgacc gcgaacccca gca
//
LOCUS       DASSRL010000597         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1275678, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000597 DASSRL010000000
VERSION     DASSRL010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275678"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFL49_12160"
     CDS             <1..667
                     /locus_tag="VFL49_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7912779.1"
                     /translation="EPPCLDRTRPEDVRADGKALAAIRRLDARQLDDIQQEQIVSAMN
                     EIETLLASTPTAKADVRPTYMLREPRAGDFGWVVSRHAELYLREYGWGDPFEGLCAQI
                     VADFVNNFDPKRERCWIAEMNGENVGCVMLVKDEQPNVARLRLLLVDPKARGLGLGAK
                     LVDESVRFARSKGYAKVTLWTHSVLSAARHIYEDAGFRLTSSQPRRTWGKDVVAEFWD
                     LEL"
     gene            complement(750..1313)
                     /gene="moaB"
                     /locus_tag="VFL49_12165"
     CDS             complement(750..1313)
                     /gene="moaB"
                     /locus_tag="VFL49_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HET7912780.1"
                     /translation="MPGIDQSRQFVPLKIAVLTVSDTREASDDTSGATLIERIRAAGH
                     VVAERAIVKDEVRAIRQHVKAWIADRFIDVVITTGGTGFTGRDVTPEAVEPLFEKKMD
                     GFATMFLLVSERKIGTSAIQTRATAGVANATYIFCLPGSPGACRDAWDEILVHQLDYR
                     YRPCNFVEILPRLDEHLRRPKAKGATA"
     gene            complement(1324..2241)
                     /locus_tag="VFL49_12170"
     CDS             complement(1324..2241)
                     /locus_tag="VFL49_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET7912781.1"
                     /translation="MRWDDFRRSDNVEDSRGDEGGFGGGGVGIPVGGGGLGIGTIVVL
                     GLIGWALGIDPSILIGGAEMVNSTRHYQQPYEPSQPRQAGAPKDQMGDFVAAVLGNTE
                     DTWTEIFKSAGQQYRVPRLRLYSGAIQGGCGFAQAAMGPFYCPNDRRIYLDTSFFRDM
                     QVRFRGCSGKACEFAEAYVIAHEVGHHVQNLLGILPKAQSAQREVGSRAEANRIQVRV
                     ELQADCLAGVWAHHSNQRWKSIEPGDVEAALQTAAAIGDDRLQQQSRGYVVPDAFTHG
                     SSAQRQRWFTIGLKEGKVSACNTFAATSL"
     gene            2476..3615
                     /locus_tag="VFL49_12175"
     CDS             2476..3615
                     /locus_tag="VFL49_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET7912782.1"
                     /translation="MVVSRRGAPKRAPRGVSESPQPHRVLASDCIAGLTTLPAASVDL
                     VFADPPYTLQLASELKRPDDSRVDAVDDDWDKFASFSAYDDFTSGWLTACRRVMKPAA
                     TIWVIGSYHNIFRVGTIMQDLGFWILNDVIWRKANPMPNFRGRRFTNAHETLIWASRD
                     ADARGYTFNYEILKAGNEDVQVRSDWTLPLCTGEERLKGRDGKKLHPTQKPESLLARV
                     ILSSSRPDDLVLDPFCGTGTTGAVAKRLGRRFIGCEQDDKYARAAEQRISRVKPLEAP
                     TIAPFVTAREAPRVPFAALIERGLVTPGARLVDAKKRHKALVRADGAVSLDGTVGSIH
                     RIGALAQGLEACNGWTFWHVETPKGLTPIDAFRAVIRSEMADAAE"
     gene            complement(3622..>4031)
                     /locus_tag="VFL49_12180"
     CDS             complement(3622..>4031)
                     /locus_tag="VFL49_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET7912783.1"
                     /translation="ESSTGGLVAAALLAVPGASAYFLGGAVVYTKSARAALLGIGDAE
                     MQGLRPATETYSLLLARLARERHGATWGFGETGATGPSGNRYGDPAGHTCLAAAGPTE
                     RTITLRTGSADRLANMNAFAKRSLELLVEVIAA"
BASE COUNT          753 a         1310 c         1216 g          752 t
ORIGIN      
        1 agagccacct tgccttgacc gcacgcggcc ggaagatgtt cgcgccgatg gaaaagcgct
       61 cgcagcaata cgccgcctcg atgctcgcca gctcgatgat atacaacagg agcaaatcgt
      121 ttccgcgatg aacgagatcg agacattgct ggcgagcacg cctacggcga aagccgacgt
      181 acgaccgacc tatatgttgc gcgagccgcg cgccggcgat ttcggctggg tcgtgtcgcg
      241 gcacgccgaa ctttatttgc gcgaatatgg ctggggcgat ccattcgagg ggctgtgcgc
      301 gcaaatcgtc gccgatttcg tcaacaattt cgaccctaag cgcgaacgat gctggatcgc
      361 cgaaatgaac ggcgagaatg tcggctgtgt catgctggtg aaagacgaac agcccaatgt
      421 cgcgcgcctg cgcctgctgc tggtcgaccc gaaagcgcgt ggacttgggc tgggtgccaa
      481 gctcgtcgac gagagcgtgc gctttgcacg gtccaaaggt tacgcaaagg tcaccttatg
      541 gacgcatagt gtgctttccg cagcacggca catctatgag gatgcgggat tcaggctcac
      601 gtcgagccaa cctcggcgca cctggggcaa ggacgtcgtc gccgagttct gggatctcga
      661 gctctaaggg cacgagagcc aatccgtaga ggttgatgct ctccaccgtc atggccgggc
      721 acgacgagtc cacttgaaag gattcggctc taagccgtcg cacctttcgc tttaggacgt
      781 cgcagatgct cgtcgagcct cggcaggatc tccacgaaat tgcacggccg gtagcggtaa
      841 tcgagctggt gcaccaaaat ttcgtcccag gcatcgcggc aggcacccgg tgagcccggc
      901 agacagaaga tgtaagtcgc gttggcgacg ccggctgtgg cccttgtctg gatcgccgat
      961 gtaccgatct ttctttcgct gaccagcagg aacatcgtcg caaacccgtc cattttcttc
     1021 tcgaaaagcg gctcgaccgc ctccggcgtt acgtcgcggc cggtaaagcc cgtgccgccg
     1081 gtggtgatga ctacatcaat gaacctgtcg gcgatccagg ccttgacgtg ctgacggatc
     1141 gccctgacct cgtctttcac gatcgcacgc tcggccacca catgacctgc tgcgcggatg
     1201 cgttcgatca gcgtcgcgcc ggacgtgtcg tcgcttgcct cgcgcgtgtc cgaaaccgtc
     1261 aacaccgcaa ttttcagcgg cacaaactgt cggctttgat cgattccggg catgtgtcgc
     1321 ttcctagaga gaggttgcgg cgaaggtatt gcaggccgaa accttgccct ccttcaggcc
     1381 gatcgtgaac cagcgctgcc tctgcgccga cgagccgtgc gtgaaggcat cgggcacgac
     1441 ataaccgcgc gactgctgct gcaggcggtc gtcgccgatc gcagccgccg tctgcaacgc
     1501 cgcttccacg tcgcccggct cgatcgattt ccaccgctga ttcgaatggt gcgcccagac
     1561 gccggccagg caatcagcct gcagctcgac gcgcacctgg atgcggtttg cttcggcgcg
     1621 gcttcccacc tcgcgttgcg ccgattgcgc tttcggcagg atgccgagca ggttctgtac
     1681 gtgatgcccg acctcgtgcg cgatcacata ggcttccgca aattcgcagg ccttgccgct
     1741 gcaaccgcgg aagcgaacct gcatgtcgcg gaagaaagag gtatcaagat agatgcgccg
     1801 gtcattcggg caatagaacg gacccattgc cgcttgcgcg aatccgcagc cgccctggat
     1861 cgctcctgag tacagccgca atcgcggcac acgatactgc tgccctgcgc tcttgaagat
     1921 ttcagtccag gtatcctcgg tattgccgag gaccgcggcg acaaagtcac ccatctggtc
     1981 cttcggcgca ccggcctgcc gcggctggct cggttcgtag ggctgctgat agtgcctcgt
     2041 actgttcacc atctcggcgc cgccgatcag gatgcttgga tcgattccga gcgcccaacc
     2101 gatcaagcca agcacgacga tcgttccgat accgaggccg ccacccccga ccggaatacc
     2161 gacaccgccg ccgccgaaac cgccttcgtc gccgcggctg tcttcaacgt tgtcgctgcg
     2221 gcggaaatca tcccaacgca tcgcaaattc ctccaccact ttgcgcgatc atcgccgaac
     2281 tgcgcgcgcg cgtccaacac cggccgggtc tttgcgccat cgtcatacct ctaagcccag
     2341 atcaaatagg cagaatttgc ccagtttcga gccgcgctca taaaccggtt ttttaccttg
     2401 gacgcgcaag atgcaggcct gtcggttgta ggtcccgcgt cgccgaccgc gtcacctgag
     2461 tcagagtcct gagtcatggt tgtgtcgcgt cgcggggcgc ccaaaagggc gccccgtgga
     2521 gtatcagaga gtccccaacc gcatcgcgtt cttgccagcg actgcatcgc aggattgacg
     2581 acgttgcctg cggcctccgt cgatctcgtc ttcgctgatc ctccctacac tcttcagctc
     2641 gcaagtgaac tcaagcggcc ggacgattcg cgcgtcgacg ccgtcgatga cgactgggac
     2701 aagtttgcga gcttctccgc ctacgatgat tttacaagcg gctggctcac cgcgtgccgg
     2761 cgcgtgatga aaccggctgc caccatctgg gtcatcggtt cctatcacaa catcttccgg
     2821 gtcggcacga tcatgcagga tctcggcttc tggatcctca acgacgtgat ctggcgcaaa
     2881 gcgaacccga tgccgaattt ccgcgggcgg cgtttcacca acgcgcatga gacgctgatc
     2941 tgggcgtcgc gcgatgccga tgcgcgcggg tacacgttca actatgagat cctcaaggca
     3001 ggcaacgaag atgtgcaggt tcgttccgac tggacgctgc cgctttgcac cggggaagag
     3061 cggctcaaag gccgcgacgg caaaaagctg catccaaccc agaagccgga atcgctgctt
     3121 gcacgcgtga tcctgtcaag ctcgcgcccg gacgacctgg tgctcgaccc gttctgcggc
     3181 accggcacga ccggcgccgt tgccaagcgg ctggggcggc gcttcatcgg ctgcgagcag
     3241 gatgacaaat atgccagggc cgccgagcag cgcatctcca gggtaaaacc gctggaagcg
     3301 ccgacaattg ccccgttcgt cacagcgcgc gaggcgccgc gtgttccctt cgcagcgctg
     3361 atcgaacgtg gcctggttac gcccggcgcg cggctggtcg atgccaagaa gcggcacaag
     3421 gcgctcgtgc gtgccgacgg cgcggtctcc ctcgacggga cggtcggctc gatccatcgt
     3481 atcggtgcgt tggcgcaagg cctcgaagcg tgcaacggct ggaccttctg gcacgtggaa
     3541 acgccgaaag gcctcacgcc gatcgacgcc ttccgcgccg tcattcgctc cgagatggcc
     3601 gacgccgccg agtaaatcgc ctcaagcggc gatcacttcg acaagaagtt cgagcgagcg
     3661 cttcgcgaaa gcgttcatgt tcgcaagcct gtcggcgctg ccggtacgca gtgtgatcgt
     3721 tctctccgtt gggccggctg cggccaggca ggtatgtccc gccggatcgc cgtagcggtt
     3781 gccggatggt ccggtcgcac ccgtttcgcc gaagccccag gttgcgccgt ggcgctcgcg
     3841 cgccaaccgc gcgagcaaca gcgaataggt ctctgtcgca ggacgcaagc cctgcatttc
     3901 cgcatcgccg atgccgagca gcgcggcacg cgccgacttt gtatagacca ccgcgccacc
     3961 gagaaaataa gccgaggcgc cgggcacagc gagcagggcc gctgcaacaa gacctccggt
     4021 cgacgattcc g
//
LOCUS       DASSRL010000598         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1188329, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000598 DASSRL010000000
VERSION     DASSRL010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188329"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFL49_12185"
     CDS             <1..338
                     /locus_tag="VFL49_12185"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7912784.1"
                     /translation="IKAYVVSDDERSPALPDVPSAKEAGDAPYMFNIWSGIYAPKSTP
                     KEAVDKLADALNKTLDDPETAKKLIALGGTVPPKAERGPDHLAKILKDDIARWDPILK
                     DAAASQKQN"
     gene            340..2223
                     /locus_tag="VFL49_12190"
     CDS             340..2223
                     /locus_tag="VFL49_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7912785.1"
                     /translation="MLTRSKADSSMRARARAIRAAASLAAALEKGPVEKLVETTLSEA
                     LVLGLLKQGVRKYFAIFGHGSTDLGNVLRIYEEEGVTRTINCRNEVEMAHAATALRWQ
                     YGELSAVVTSIGPGAMQAFAGSLAAASNGVGVYHIYGDETTFGEGYNMQQVPKEEQGL
                     FGRMTAVMGQSYVLHTPEALRDALRRGSLCVNHPYRAGPFYLLLPLNTQPAIVTVNLA
                     ALPARPQLPKLAPADDAAIDHAASMIAKAAKVAIKAGGGTRGYDAAVRRLAESAGAAV
                     ILSPGSTGVLPDTHAQNMHVGGSKGSISGNYAMAEAELLIVIGSRAVCQADCSGIGYK
                     NAQAVININGDLADALHYNKTMALVGDVGAVADRLSGSLAALKANGGKTSWLEACAAK
                     KAEWAAYKRKRYACGPLHDEAWQKPVLTQPAAIKLVADFAKEIGAAKFFDAGDVQANG
                     FQIVEDDRTGDTFTESGASYMGFAASALLGGALAEKRRYGIAFCGDGSFMMNPQILID
                     AVEHSVRGMIVIFDNRRMAAISGLQLAQYQAEFRTNDHVAVDYRAIAAAVSGVLAISG
                     GDNIESLRRALKSAHAHDGLSVIHVPVYSGPDELGGLGAWGEWNVGNWCEDVQGAWIR
                     QDL"
     gene            2269..2883
                     /locus_tag="VFL49_12195"
     CDS             2269..2883
                     /locus_tag="VFL49_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891586.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET7912786.1"
                     /translation="MVESTPVKSLKALRDAEKACTRCPLYKHATQAVPGEGPARARIM
                     LVGEQPGDQEDKQGKPFVGPAGSVLAKALSDAGIERKAVFVTNAVKHFKFEPRGKRRL
                     HKRPNAYEIDRCHWWLDFERKLIKPDIVVALGATAVRSVSGRPLSISRIRGRILPLPD
                     GGRMIATIHPSYVLRIEDEADKRTQYQKFVADLKVCAKVLAQAA"
     gene            complement(2880..>3392)
                     /locus_tag="VFL49_12200"
     CDS             complement(2880..>3392)
                     /locus_tag="VFL49_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7912787.1"
                     /translation="AKPDAIKNLYAGKLYLAGGSHGEKLMYALGAEFVEVRVHSQTRE
                     IRVPRIVGAFAAGRLMNTRTARSQYMGAMIWGISSALHEATEIDPRVARYVNDNLADY
                     MIPVNADIQQLDVILVPEEDTLVNPAGVKGIGELGNVGTAAAVANAVYHATGIRVRQL
                     PIRLEKLLAV"
BASE COUNT          671 a         1017 c         1073 g          631 t
ORIGIN      
        1 agatcaaggc ctacgtcgtg tcggatgacg agcgttcgcc ggcgcttccg gacgtaccgt
       61 ccgccaagga ggcgggtgac gcgccgtaca tgttcaacat ctggagcggc atctacgcgc
      121 cgaagagcac gccgaaggag gctgtcgaca agctggccga cgcgctcaac aagacgctgg
      181 acgatcccga aaccgccaag aagctgatcg cgcttggcgg caccgtgccg cccaaggccg
      241 agcgcggtcc ggatcatctc gccaagatcc tgaaggacga catcgcccgc tgggatccga
      301 tcctgaagga cgccgcagca tcgcagaagc agaactgacg tgctgacacg ctcgaaagcc
      361 gacagctcga tgcgggcccg cgcgcgcgcc attcgcgccg cggcctcgct cgccgccgcg
      421 ctcgagaagg gcccggtcga gaagctcgtc gaaaccacgt tgtccgaagc cctcgtgctc
      481 ggcctgctga aacagggcgt gcgcaagtac ttcgccattt ttggtcacgg ctcgaccgat
      541 ctcggcaatg tgctgcgcat ctacgaggag gaaggcgtca cccgcacgat caattgccgc
      601 aacgaagtcg aaatggcgca tgccgcgacg gcgttgcgct ggcaatacgg cgagctctcg
      661 gcagtcgtca cgtcgatcgg gcctggcgcc atgcaggctt tcgccggctc gcttgccgcc
      721 gcatcgaatg gggtcggcgt ctaccacatc tacggtgacg aaacgacctt cggcgaaggc
      781 tacaatatgc agcaggtgcc gaaggaggag cagggcctgt tcggccgcat gacggccgtt
      841 atgggtcagt cctatgtcct tcatacgccg gaagcgctgc gcgacgcgct gcgccgcgga
      901 agcttgtgcg tgaaccatcc ttatcgcgca ggcccgttct atcttctcct gccgctcaat
      961 acccagccgg cgatagtcac cgtgaatctt gccgcgctgc cggcacggcc gcagctgccg
     1021 aaactcgcgc ctgcagatga cgcggcaatc gatcatgcgg ccagcatgat cgccaaggct
     1081 gcaaaggtcg cgatcaaggc cggcggcggc acgcgcgggt acgatgcagc ggtgcggcgg
     1141 ctggcggaat ccgcgggtgc ggccgtcatt ctgtcgcctg gctcgaccgg agtgctgccg
     1201 gacacgcatg cacagaacat gcacgtcggc ggctccaagg gctcaatcag cggcaattat
     1261 gcgatggccg aagccgaatt gctgattgtc atcggctcgc gtgccgtctg ccaggccgat
     1321 tgctccggga tcggatataa aaacgcccag gcggttatca acattaacgg cgatctggcg
     1381 gatgcgcttc attacaacaa aacaatggcc cttgttggcg acgtgggcgc ggtcgccgat
     1441 cgtctttccg gcagccttgc ggccctgaag gcgaacggcg gcaagacatc gtggctcgag
     1501 gcctgcgccg ccaagaaagc cgagtgggcg gcctacaagc ggaagcgtta cgcctgcgga
     1561 ccgctgcacg atgaagcgtg gcagaagccg gtgctgacgc agccggcggc catcaagctt
     1621 gttgccgact ttgcgaaaga aattggtgca gcgaaattct tcgatgccgg cgacgtgcag
     1681 gcaaacggct tccagatcgt cgaggatgac cgcaccggtg atacgttcac tgaatccggc
     1741 gcctcatata tggggttcgc cgcaagcgcc ctgctcggcg gcgcgctggc tgaaaagcgg
     1801 cgctacggaa tcgcgttttg cggggacggt tcatttatga tgaaccctca gattctcatt
     1861 gacgccgtcg agcacagcgt gcgtggcatg attgtcattt tcgacaatcg gagaatggcc
     1921 gcaatctcag gcttgcagct ggcgcagtat caggccgaat tccgtactaa cgatcatgtt
     1981 gcggtcgact acagagctat cgctgccgcg gtgtcgggcg ttctcgcgat ttccggcggc
     2041 gacaatatcg aatcgttgcg aagggccttg aaatccgcgc acgcgcacga tggtctgtcg
     2101 gtgatccacg tgcccgtata ttccggaccg gacgagcttg gcggcctggg cgcgtggggc
     2161 gaatggaacg tcggcaactg gtgcgaagac gtgcagggtg cctggatcag gcaggatctt
     2221 taagggaacc gttgcgagcg cctttcgttg ccgctgcgga ggcgttacgt ggtcgagtca
     2281 acacccgtca aatcgctgaa agcactacgt gacgccgaaa aagcatgcac gcgctgcccc
     2341 ctctacaaac atgcaacgca ggctgtgccg ggtgagggac ctgcgcgggc acgcattatg
     2401 ctggtcggtg agcaacccgg cgaccaggaa gacaagcagg gcaagccatt tgtcgggccc
     2461 gccggcagcg tgcttgcgaa agcgctaagc gacgctggca tcgaacgaaa agccgtcttt
     2521 gtcacgaacg cggtcaaaca tttcaaattc gagcctcgcg gcaagagacg cctgcacaaa
     2581 cgtccgaatg cctacgagat cgaccgttgt cactggtggc tcgatttcga gcggaagctg
     2641 atcaagccgg atattgtcgt cgcgcttggg gcgactgccg tgcgaagcgt ttccggccgc
     2701 ccgttgagta taagcaggat ccggggccgc attttgccgc ttccggatgg aggccgcatg
     2761 atcgcaacga ttcacccctc ctacgttctg cgcattgagg atgaagccga caaacgtacg
     2821 caatatcaaa aatttgttgc cgacctcaag gtctgcgcaa aggtcctcgc ccaggcggcc
     2881 tagacggcga gaagtttttc gaggcgaatg ggcagctgcc gtacacgaat tccggttgcg
     2941 tggtacacgg cgtttgcaac tgccgcggca gtgccgacat tgccaagctc gccgatgcct
     3001 ttgacgcccg ccggattcac caaggtgtct tcctccggaa ctagaatgac atcgagctgc
     3061 tggatgtccg cgtttacggg gatcatgtaa tccgcaaggt tgtcgttgac gtaacgcgca
     3121 acgcgcggat cgatctcggt cgcttcgtgc agggccgagc tgatgcccca gatcatcgca
     3181 cccatatatt ggcttcgtgc ggtgcgcgtg ttcatcaagc ggccggctgc aaatgcgccg
     3241 acgatgcgcg gcactcgtat ttcccgcgtt tgagagtgca cgcggacttc gacaaattcg
     3301 gcgccgagcg cgtacatcag cttttcgccg tgcgagcccc cggcaaggta aagttttccg
     3361 gcatagagat tttttattgc gtcgggtttg gc
//
LOCUS       DASSRL010000599         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_138046, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000599 DASSRL010000000
VERSION     DASSRL010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138046"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFL49_12205"
     CDS             complement(<1..239)
                     /locus_tag="VFL49_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7912788.1"
                     /translation="MMSREQNEQLSRVGPGTLMGKLLRRYWAPFLLSAEIPEPDCPPV
                     RVKLMGESLVCFRDSKGQIGLIDEFCAHRGVSLWFG"
     gene            327..1328
                     /locus_tag="VFL49_12210"
     CDS             327..1328
                     /locus_tag="VFL49_12210"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7912789.1"
                     /translation="MPLPRIAIATGDPAGIGPEIALKAALDTRVRDLCRPLLVGDPIA
                     VEMHSRAAGLSPDLHVVRQPSEAKWSDDTVNLLDASRGNNAPIKIGTIDAAYGRASLA
                     SASRAIKAALAGDVEAVVAAPQTERSIAAAGIAFDGYPSFVARETGTPVNDVYLMICF
                     DESRIVHATLHVSVRDAVALITGERVERVIRVTDASLRKLGVAQPKIFVSGLNPHAGE
                     DGLFGSEEIDIIIPAIARAAQDGIAVSGPFGADTMFRKKGCDAFIVMLHDQGHIAAKL
                     LAFNRTAGLSIGSPILFSSVAHGSAHDIAGKGQADPGAMIEAITRLARVNARDRVRD"
     gene            complement(1318..1665)
                     /locus_tag="VFL49_12215"
     CDS             complement(1318..1665)
                     /locus_tag="VFL49_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7912790.1"
                     /translation="MIARALIMLSVAIVFAQNPAVATSAEHGKAAFMQHGCWQCHGTV
                     GQGSIATSGGKRLAPDPMPWETFSAFVRSSNRAMPPYSEKILTDGDLEDIYAYLQSIP
                     RPRDAKTIPLLNP"
     gene            complement(1676..>2493)
                     /locus_tag="VFL49_12220"
     CDS             complement(1676..>2493)
                     /locus_tag="VFL49_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7912791.1"
                     /translation="NTKSNYQDFQRLQVVDIEMAADAEATLPALIDAVKQALPSSRNA
                     AIEARGTALKKAWSEARDRTRQAAAYAWDASPIGTARMAMEVYAQIKDLDWSSVGGDR
                     QLSSWPSRLWPMDKHYHHIGGPGGYGIGYGAPAAVGAALANRDAGRFSVNFQPDGDMM
                     YSPGVLWTAVHHQIPLLTVMHNNRGYHQEVMHVQRLANRRNRVAYAGKDLGPIGTRIE
                     NPLIDYGKLAGSLGMWSAGPIADPNDLAPALKKAVQVVKAGEPALVDVVTQPR"
BASE COUNT          444 a          827 c          767 g          455 t
ORIGIN      
        1 ccgaaccaga gcgagacgcc gcgatgcgca caaaattcgt cgatcaggcc gatctgcccc
       61 ttgctgtcgc gaaagcagac caggctttcg cccatcagct tcacccgcac gggcggacag
      121 tccggctcgg gtatttcagc ggagagcaga aacggcgccc agtatcggcg aagcaatttg
      181 cccatcaatg tcccggggcc gacacggctg agctgctcgt tctgttcacg cgacatcatg
      241 gcgacgcttc ctcgaggctt tgctatgctc ttgcggttgc tcgacgccat tataggcagc
      301 cgcaccggcg acggaagtca accgccatgc ccctaccgcg catcgccatt gcaacgggag
      361 atcccgccgg catcggcccc gagatcgcgc tcaaggccgc gctcgatacg cgcgtgcgtg
      421 acctctgccg gcccttgctg gtcggcgatc cgattgcggt cgaaatgcat tcccgcgccg
      481 ccggcctgtc gcccgacctt cacgtcgtac gacaaccatc cgaagcaaaa tggtccgatg
      541 acaccgtcaa tctcctggat gcgagccgcg gcaacaatgc cccgataaaa atcggcacca
      601 tcgacgccgc ctatggccgc gcttcactgg cgagcgcgag ccgggcaatc aaggccgcgc
      661 ttgccggcga cgtcgaggcg gtcgtcgcgg cgccacagac cgagcgctcc atcgcggcgg
      721 caggcatcgc cttcgacggc tatccatcgt tcgttgcgcg cgagaccggc acgccggtta
      781 acgacgtcta cctgatgatc tgtttcgacg agtcgcgcat cgtgcacgcg accttgcacg
      841 tgagcgtgcg cgacgccgta gcgctgatca cgggcgagcg cgtcgagcgg gtgatccgcg
      901 tcaccgacgc gtcgctgagg aaactcggcg ttgcacagcc gaagatattc gtgagcgggc
      961 tcaatcctca cgccggagag gatggcctct ttggctcgga ggagatcgac atcatcattc
     1021 cagcgatcgc gcgagcggcg caggacggca tcgctgtgtc gggaccgttt ggcgccgaca
     1081 ccatgtttcg aaagaaagga tgcgatgcat tcatcgtgat gctgcacgac cagggccaca
     1141 tcgccgccaa gctgctggcc ttcaaccgca ccgcgggact ttcgatcggc tcgccgatcc
     1201 tgttctcatc ggtcgcgcac ggcagcgcgc acgacatcgc gggcaagggt caagccgacc
     1261 ccggcgccat gatcgaggcg atcacacgac tcgctcgcgt caacgcgcga gaccgcgtca
     1321 gggattgagc agcggaatcg tctttgcgtc gcgcggcctt ggtatcgact gcaggtacgc
     1381 gtagatatcc tcaagatcgc cgtcggtcag aatcttttcg ctatagggcg gcatggcgcg
     1441 gttgctcgac cgcacgaatg cggaaaacgt ctcccacggc ataggatcgg gcgccaggcg
     1501 tttgccgccg ctcgttgcga tcgaaccctg acctacggtg ccgtggcatt gccagcagcc
     1561 gtgctgcatg aacgctgctt tgccatgctc agccgatgtg gccactgccg gattctgcgc
     1621 aaaaacaatt gccactgaca gcatgatcaa cgcgcgcgct atcattgcac gatcctcagc
     1681 gtggctgcgt gacgacgtca accagcgcag gctcgccggc cttgacgact tgcaccgcct
     1741 ttttcagcgc aggtgccaga tcgttcgggt cggcaatcgg cccggcggac cacatgccga
     1801 gcgagcccgc aagcttgccg tagtcgatga ggggattctc gatcctggtg ccgatcggcc
     1861 cgaggtcctt gcccgcatag gcaacgcggt tgcgccggtt cgccagacgt tgaacatgca
     1921 tcacctcctg atgatagccg cggttgttat gcatcaccgt caggagggga atttggtgat
     1981 gcacagcagt ccacaacaca cccggcgagt acatcatgtc gccatcgggc tggaagttga
     2041 ccgagaagcg gccggcgtcg cggttggcca gggcggcgcc taccgcagcc ggcgcgccgt
     2101 agccgattcc gtagccgccc gggccgccga tgtggtgata gtgcttgtcc atcggccaca
     2161 accggctcgg ccagcttgac agctggcgat cgccgccaac cgacgaccag tcgagatcct
     2221 tgatctgggc gtaaacttcc atcgccatgc gtgccgtacc gatcgggctc gcgtcccagg
     2281 cgtaggcggc ggcctgtcgc gtgcggtcgc gcgcttcgct ccatgctttt ttcaacgcag
     2341 tgcctcgcgc ctcgatcgcc gcgttgcggc tcgacggcag cgcctgcttc acggcgtcga
     2401 tcagcgcggg cagcgtcgcc tccgcatcgg ccgccatttc gatatccacg acctgtaatc
     2461 gctgaaagtc ctggtagttc gactttgtgt tga
//
LOCUS       DASSRL010000600         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1748146, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000600 DASSRL010000000
VERSION     DASSRL010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748146"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1261)
                     /locus_tag="VFL49_12225"
     CDS             complement(62..1261)
                     /locus_tag="VFL49_12225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HET7912792.1"
                     /translation="MPGAGTDKTKRWIETPAPVVVLVEPQLGENIGAAARAMANFGLS
                     QLRLVKPQQKWPNEKARVMAAGADRILDAATLYETLNEAIGNCSFVVATTARNHDQAK
                     PVISAEAAAAEMAPRVAGGENVAIVFGRERIGLENHEVALADRIVTLPVNPAFASLNL
                     AQAVVIVAYEWFKSAGGGDLPFSMPEKSPPAPKQQLDAFFSDLERELERVEFFRPEEK
                     RGTMSVNLRNIFQRMAPTQQDIRTLHGVLNAIAQGRKGPARGGVLDPVEAQKLRDLLA
                     EHGGGRVPGERGPVRGLARLLRRNPTEAERILWQALVNDRRFAGRGFKRQVPIGPHIT
                     DFVSFPLKCVIDVVPAGETDDAARTRAERRTYLRERGYKVCEVRGAEVGKDAGRVLDR
                     LHELVGQ"
     gene            1332..>2477
                     /locus_tag="VFL49_12230"
     CDS             1332..>2477
                     /locus_tag="VFL49_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7912793.1"
                     /translation="MPQLILGVHSGSHDAAAAVFEDYSLKAAVHLERLTRRKSDGSGH
                     PDAAIDEVLSIARATRRDVDVVAYSRSMFPTRYFRHLHGLEWLREQYRTYLRRRPRRQ
                     LMPELFRHQTADVRVFLDVDSLQRDSGFRPGVATTFYNHHEGHALPALFYSPWDNALV
                     VTADGGGDNVHYSHRHFTADRLTSLYGGDDMILMPAEEDSLGKMYGWATKALGFKRVR
                     HEGKVTGLAAMGTPVRADELRSHYRIDDQGRVHSDLGGDRAIFAFMRALKRGLSREDY
                     AATVQHVLEDVMLKSIQQLLRNNPARHLGLSGGVFANVKLNRLLAETLDLDEIFIFPA
                     MGDDGLPVGGALAWLLQRDGLAHWLKQRRDLGDLYLGRDFSGSVDTEF"
BASE COUNT          427 a          813 c          752 g          485 t
ORIGIN      
        1 caacgaccac acgaccctgc gctcggccac ggttgcgcag ctgaaggtgt tgaaggggta
       61 actactgccc gaccagctcg tggagccgat cgagcactct gcctgcgtct tttcccacct
      121 cggcgccccg tacttcgcac actttgtagc cacgctctct caggtaggtg cgcctttcgg
      181 cccgcgtccg cgctgcgtcg tcggtttcac cggctgggac cacgtcgatc acacacttca
      241 gcggaaacga cacgaagtcg gtgatatgcg ggccgatcgg aacctggcgc ttgaagccgc
      301 ggccggcgaa acggcgatca ttcaccagtg cttgccacag gatgcgttcg gcctcggtcg
      361 gattccgacg cagcagccgc gccagcccgc gcaccggacc gcgctcgccc ggcacgcggc
      421 ccccgccgtg ctccgccaac aggtcgcgca gcttctgggc ttccaccggg tcgagcacgc
      481 cgccgcgcgc cggccccttg cgtccctggg cgatcgcgtt aagcacgccg tgcagcgtgc
      541 ggatgtcctg ctgcgtcggc gccatccgct gaaaaatatt gcgcagattg acgctcatcg
      601 tgccgcgctt ttcttcggga cggaagaatt cgacccgctc aagctcgcgt tcgagatctg
      661 aaaaaaaggc atcgagctgc tgcttaggcg ccggcggcga tttttccggc atggagaacg
      721 gcaggtcgcc gcctccggcg gatttgaacc attcgtaggc gacgatcacc acggcctgcg
      781 ccaggttcag tgaagcgaag gccggattta ccggcagcgt cacgatccgg tcggccagcg
      841 caacctcgtg attttcgaga ccgatgcgtt ctcgaccaaa cacgatggca acattctctc
      901 cgccagccac tctcggcgcc atttcggccg cagccgcttc cgccgaaatc accggtttgg
      961 cctgatcgtg attgcgcgcg gtcgtcgcca cgacgaaact gcaattgccg attgcctcgt
     1021 tgagggtttc ataaagcgtc gcggcgtcca gaatacgatc ggctccggca gccatcacgc
     1081 gggctttctc gttcggccat ttttgctgcg gcttcaccag ccgcagctgt gacagcccga
     1141 aattggccat cgcgcgcgcg gccgctccaa tgttctcgcc gagctgcggt tcgacaagga
     1201 cgacgacggg tgcgggcgtt tcgatccagc gtttggtctt gtcggttccg gcgccgggca
     1261 ttgtcgattc ggttgtcgct gcgtacggag tgtggcaatg attgccggtt ttcaaatcct
     1321 gtcctggcaa catgccgcaa ttgatattgg gcgtccattc cgggtcgcac gacgccgcgg
     1381 ctgcggtctt tgaggactat tcgctcaagg cggcggttca cctggaacgc ctgacccgcc
     1441 gcaagagcga cggatcgggc catcccgatg ctgcgatcga tgaagttcta tcgatcgcga
     1501 gggcgacgcg gcgcgacgtc gatgtcgtgg cctacagccg cagcatgttc ccgacgcgct
     1561 attttcgtca cctgcacggc ctagaatggc tgcgcgaaca gtaccgcacc tacctgcgcc
     1621 gcaggccacg gcgacagctg atgccggagc tctttcggca tcagacagcc gacgttcgcg
     1681 tgttcctgga cgtcgattcc ctccagcgcg acagcggatt tcgtcccggt gttgcaacga
     1741 ctttctacaa tcaccacgaa ggtcacgccc tgcccgcgct tttttatagc ccttgggaca
     1801 acgcgctcgt cgtgaccgcg gacggcggcg gcgacaacgt ccattacagt caccgccatt
     1861 ttaccgccga caggctcaca tccctttatg gcggcgacga catgattttg atgccggctg
     1921 aagaagacag cctcggcaag atgtatggct gggcgaccaa ggcactcggt ttcaagcgcg
     1981 tgcgccacga aggcaaggtg accgggttgg ccgccatggg cacgccggtg cgcgccgacg
     2041 aactccggtc gcattatcgc atcgacgacc agggaagggt gcattcggat cttggcggcg
     2101 atcgcgcgat ctttgccttc atgcgcgcgc tgaagcgcgg actcagtcgc gaggactatg
     2161 cagccaccgt gcagcacgtg ctggaagacg tcatgctgaa gtccattcag caactgctca
     2221 ggaacaatcc ggcgcgacac ctgggccttt ccggcggcgt tttcgcaaac gtcaagctca
     2281 accgtttgct cgcggagacg ctcgacctcg acgagatttt catattcccg gccatgggcg
     2341 acgacggctt gccggtcggc ggcgccctcg cgtggctgtt gcaacgcgac ggacttgcgc
     2401 actggctcaa gcagcgccgc gatcttggcg acctttacct ggggcgcgac ttttccggca
     2461 gcgtcgacac cgaattc
//
LOCUS       DASSRL010000601         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_890651, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000601 DASSRL010000000
VERSION     DASSRL010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890651"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1226
                     /locus_tag="VFL49_12235"
                     /pseudo
     CDS             57..1226
                     /locus_tag="VFL49_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1237..>2094
                     /locus_tag="VFL49_12240"
     CDS             1237..>2094
                     /locus_tag="VFL49_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET7912794.1"
                     /translation="MILGETNRASGITTTLDDLFRRAGVRRPDAPALVDPPNRSGFTD
                     GPPRTLSYAEADRAISAVAARLRGLGLQTDAVVAIQLPNTVESVVAYLGVLRAGMIAA
                     PLPLLWRRRDLVDCLGRVSAKAIVTCARVGRAAPAELAMHTAAALFPIRHIFGFGQNL
                     PDGIVPIDAAFAPGDTDAAAMPVRPDSAATHVAAITFGVDARGIAPAARNHTEFISGG
                     LAVFLEAGIAPNACLLSTIPISSFAGAALTLVPWLLSGGTLHLHHGFDSTAFAAQRDT
                     AHCDMLALPA"
BASE COUNT          361 a          712 c          621 g          400 t
ORIGIN      
        1 tgctggccaa gcactccatc aatgttgcga ctttcgaaga cacgagcttc gatccgttgc
       61 tcggcagcgg gccatacact gtcagcaagg tcaagccggg cgaaagtgtc acattcacgc
      121 gcgacaaaaa ttactggggg aaggacctgc cgatcaaccg cggcctctgg aacttcgaca
      181 cgatccgcta tgactagtac cgcgacggca atacgcattt cgaggcgttc aagaaaggtc
      241 tctacgatct gcgcgtcgaa accgaccctg gccgttggca gaccgcctac gaatttccgg
      301 caatgcgtga tggccgtgtc gtcaaggaag acctgcccta tggattgccc aagggcatgc
      361 agggcctggt cttcaacacg aggcgctcga tcttctccga tgtccgcgtg cgcgaagcga
      421 tcctgcagct gttcgacttc gagtggatca atcgcaccta tttcttcagc ctctacaaac
      481 gaacggcgag ctacttcgac ggctgcgacc tgtccgcaca tggcgttgcg gccggcgctc
      541 gcgagcgcga gctgctggct ccgttcccgg gcatcgtccg cgcggacatc atggagggaa
      601 actggtcacc gccagtcacc gacggaacag gacacgaccg cactgtgctc cgccgcgcct
      661 tcgcactctt cagggccgcc ggctacgaac tcaagggcac ccaactgctg aataccgcaa
      721 ccgggcgacc gctgtcgttc gagatcctca ccaccacgcg cgatcaggag cgtctcgcgc
      781 tcgccttcgt ccgcaacctc aagcgcgccg gcatcgaggc gcgcgtgcgc accgtcgatg
      841 ccacccagta cgaacgccgg cgcatcgctt tcgatttcga catgatcgaa taccgctggg
      901 aacaatcctt gtcgcccggc aacgagcagc tgttctactg gggctcggcg gctgcggatc
      961 aacagggcag ccgcaactac atgggggtca aatcgaaggc ggtcgatgcc atgatcgccg
     1021 cgatgctcgg cgcaaccagc cgcgagaatt tcgtcgccgc cacccgcgcg ctcgatcgcg
     1081 tcttgctgtc aggcttctat gtggtgccgc tctatttccc gcccgtgcag tgggtggcgc
     1141 gctggacgcg aatcgcgcat ccctcgcgga cgtcgttgtt cggctacctg ccggaaacat
     1201 ggtggcagaa ggacctcaag ccctgaggcg gttgcgatga tcctcggcga gaccaaccgg
     1261 gcatccggca ttaccacgac gctcgatgat ctctttcgcc gtgccggcgt ccgacgcccg
     1321 gatgcgcccg cgcttgttga tccgccaaac cgctccggct tcactgacgg tccgccccgc
     1381 acgttgagct acgcagaggc cgaccgcgca atttccgcag tcgccgcacg tttgcgcgga
     1441 ctgggcttgc agaccgatgc ggtggtggcg atccagttgc cgaacaccgt tgaaagcgtg
     1501 gtcgcatacc tcggcgtcct gcgcgccggc atgatcgccg cgccactgcc tttgctctgg
     1561 cgccgccgcg atctggtcga ttgcctcggt cgcgtcagcg caaaggccat cgtgacatgc
     1621 gcgcgggtcg gccgcgccgc gcctgccgaa cttgccatgc acaccgcagc cgcgttgttt
     1681 ccgattcgcc acattttcgg cttcggccag aatttgcccg atggcatcgt gccgatcgac
     1741 gcggcatttg cacctggcga cacggacgct gctgcaatgc cggttcggcc ggactctgcc
     1801 gccactcacg tcgccgccat cacgttcggc gttgatgcgc ggggcatcgc tccggctgct
     1861 cgcaaccaca ccgagttcat cagcggtggg ttggcggtct ttctggaagc gggcatcgcg
     1921 cccaatgctt gtttgctgtc gaccattccg atcagcagct tcgccggtgc ggccctcacg
     1981 ctcgtaccct ggcttttatc gggcggcacg cttcacctac atcacggctt cgactcgaca
     2041 gcctttgccg cgcaacgcga cacagcgcat tgcgacatgc tggctttgcc ggcg
//
LOCUS       DASSRL010000602         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1415219, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000602 DASSRL010000000
VERSION     DASSRL010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415219"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFL49_12245"
     CDS             complement(<1..605)
                     /locus_tag="VFL49_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7912795.1"
                     /translation="MLRLYGSAKSRAVRVLWMLGELGIQYDHKDWLPRSPETKTPEYL
                     QLNANARVPTIDDDGFVLSESMAINMYLAKKHKSPLAPSDPRLEALAMQWSLWETDRL
                     DRQVVNYCRHTNDLPQAERKPDVADAAWKEMVPAFDALEAALKKSEWLAGPQFSVADL
                     NVASALFRGLTLDLGKWPKLNAWLHKCWDRPAAKKAKAMRDT"
     gene            complement(705..1670)
                     /locus_tag="VFL49_12250"
     CDS             complement(705..1670)
                     /locus_tag="VFL49_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912796.1"
                     /translation="MKQYIGWLLGAALALASIAAPAQKYPDKPIRLVVAFPTGAPYIL
                     ALMLSDKLREPMGQPVVPDFRGGAGGSLASELVAKAPADGYTLLLTSATIVISPSLYS
                     KLGYDPFRDFAPVTLIATVPNMIVVHPSVPAKSLAELVKLAKASPGKLNYGSGGVGSG
                     SQLGSELFKTINRLSMVHVPYKGAALAMTAMLGGEVDMVTTTLPAAIPLVNEKRVRAL
                     AIMAPKRAAALAGVPTTAEAGMPELVVITWYGLFVPAGVKPDVVERLHAEFTRALNAP
                     DIREKLAKVELDANPTTPADTAKFVRAEYERWGKVIRDAGIKPPQ"
     gene            complement(1899..2198)
                     /locus_tag="VFL49_12255"
     CDS             complement(1899..2198)
                     /locus_tag="VFL49_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912797.1"
                     /translation="MSDSEPISPRARLQQLQAIPERQRTDAEWDELNELEIMLHSGNR
                     QGGPNPNYRQQGPMSSGSQQQQHRQGGGQQRPGGGQQHRGQRSGRKFHQRPPKQR"
     gene            2297..>2508
                     /locus_tag="VFL49_12260"
     CDS             2297..>2508
                     /locus_tag="VFL49_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912798.1"
                     /translation="MAANYLPEVLETTEAERLATGFIFTEGPLWDPDGFWYFVDLRRN
                     HLHRMRPGQAPELYKETQGGNGTTFDL"
BASE COUNT          428 a          806 c          786 g          488 t
ORIGIN      
        1 gtatcccgca tcgccttcgc tttttttgcg gcggggcgat cccagcactt gtgcagccag
       61 gcattcagct tcggccactt gccgaggtcg agggtcagcc cgcggaacag ggcggaagcg
      121 acgttgaggt cggcgacgga gaactgcggc ccggcgagcc attccgactt cttcagcgcc
      181 gcttccagcg cgtcgaacgc cggcaccatt tccttccacg ccgcatcggc aacgtcgggc
      241 ttgcgctcgg cctgcgggag gtcgttggtg tggcggcagt aattgaccac ctggcgatcg
      301 agacggtcgg tctcccacag gctccattgc atcgccagcg cttccagcct gggatcggac
      361 ggcgcaagcg ggctcttgtg cttcttcgcg aggtacatat tgatcgccat cgactccgac
      421 aggacaaagc cgtcgtcgtc gatcgtcggc acccgggcgt tggcgttgag ctgaagatat
      481 tccggcgtct tggtttccgg cgagcgcggc agccagtcct tgtggtcgta ctgaattccc
      541 agctctccca gcatccacag cacgcgcacc gcgcgcgatt tggccgaacc atagagtcga
      601 agcatgttct gtccctttct tcctgataga tgatgcgtga tagggcaaac ctccgcccct
      661 gtgacttgtg acttgtgact tgtgccttgt gtcgtgtgcc ttctttactg cggcggtttg
      721 atgccggcgt cccggatcac cttcccccag cgctcgtact cggcgcgcac gaacttcgcg
      781 gtatccgccg gagtggtggg attggcgtcg agctcgacct tggcgagctt ctcacggata
      841 tccggggcgt tgagcgcccg ggtgaactcg gcgtgaagac gctcgacgac gtcaggcttg
      901 acgcccgcgg gaacgaacaa gccgtaccag gtgatcacca ccagctccgg cattcccgcc
      961 tcggccgtgg tcggcacccc ggcgagcgcc gccgcgcgct tcggtgccat gatcgcgagc
     1021 gcgcgcacgc gcttttcatt caccagcgga atagccgccg gcagcgtcgt cgtcaccatg
     1081 tcgacctcgc cgcccagcat cgcggtcatc gcgagcgcgg ctcccttgta ggggacgtgc
     1141 accatgctga gcctgttgat ggtcttgaag agctcgctgc ccagctggct acccgagccg
     1201 acgccgccgg aaccgtagtt gagctttccc gggctcgcct ttgcaagctt caccagctcg
     1261 gcgaggctct tcgcaggcac cgatggatgg accacgatca tgttgggaac cgtcgcgatc
     1321 agggtcaccg gggcaaaatc gcggaacgga tcgtagccga gcttgctgta taggctcgga
     1381 ctgatgacga tggtggcgga ggtcagcagc agcgtgtagc cgtctgccgg cgccttggcg
     1441 acgagctcgg aggcgagcga gccgccggcg ccgccgcgaa aatcgggcac gaccggctgg
     1501 cccatcggct cgcgcagctt gtccgaaagc atgagcgcca ggatataggg agcaccggtc
     1561 ggaaacgcca ccaccaggcg gatcggcttg tccggatatt tctgcgcggg tgcggcgatc
     1621 gatgcaagcg cgagcgccgc gcccaggagc cacccgatgt attgcttcat ccctcctcca
     1681 tccgttgccg ctgatttctt cacatcatag cggcattggt gagaaggtta aaatataaat
     1741 aaaatcaaat atttatatag ttttggtccg caatccggtc tttccgagcc gcgcgcccgg
     1801 cgcgctgcgc aaattcgggt gaaaagcggc gcgaggaaag cggaaggagc gctttaggcc
     1861 gctgacgcga caatcacagg gaaaaacagc ggagcagctc agcgctgctt cggaggacgc
     1921 tggtggaatt ttcttcccga acgctggccg cgatgctgct gaccgccacc gggcctctgc
     1981 tggccgccgc cctgccgatg ctgttgctgc tggctgccgc tcgacatcgg gccttgctgt
     2041 cgatagttgg gattcggccc cccctggcgg ttgcccgagt ggagcatgat ctcgagctca
     2101 ttcagctcgt cccactcggc gtcggtacgc tgccgctcgg gaatcgcctg gagttgctgc
     2161 aggcgcgcgc gtggagaaat tggttcagag tcgctcacat cgttattatc tcattctttt
     2221 agcggctgtt gcggcgacct tctgaaaccc gccgggccgc agcaccgtca cctgcaagag
     2281 acgccaagga gacagcatgg ccgccaacta tctgcccgaa gtcctggaaa ccaccgaagc
     2341 cgagcgtctc gccaccggct tcatcttcac cgagggcccg ctctgggatc cggacggttt
     2401 ctggtacttc gtggacctgc gccgcaacca cctgcaccgg atgcgcccgg gccaggcgcc
     2461 ggagctctac aaggaaacgc agggcggcaa cggcaccacc ttcgacct
//
LOCUS       DASSRL010000603         6204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_68242, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000603 DASSRL010000000
VERSION     DASSRL010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68242"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VFL49_12265"
     CDS             <1..1421
                     /locus_tag="VFL49_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912799.1"
                     /translation="EPCGRGVIAAEPWPHAVGREIAPQRRRMVLAACVLASSMAFIDG
                     SALTVALPKLRAYFGADLASVQWVLNGYVLVLAALTLIGGALADHYGKARMLAVGCLG
                     FGFTSVACALAPSVELLVACRVAQGIAAAILTPSSLALIGATYPKEERNRAIGVWAAA
                     SALTTAGGPVLGGWLTENFGWQWVFAINPPLALIAVALLMNFAPADQHLARHFDIAGS
                     AILAAALAALAWALSQIGPGAAASNGTAVAVVSMLGLGGLACYAAWERISAHPMTPPR
                     LTRNRAFVALNIATLLIYTGLAIMFFVLPFDFIDRRHLSPTDAGLAFLPFTLGVGFLS
                     QPFGAMADKFGARTMLIAGPSAAALALLLLALGETAPLIPGVLLPMTLLGISFALLVA
                     PLTASVMSSVTDAEEGLASGVNNAMSRVAQLSGVALSAGLASYESGFRNGLIVAAIAT
                     AAGALTIAILLPSDGGKARRTA"
     gene            complement(1442..2173)
                     /locus_tag="VFL49_12270"
     CDS             complement(1442..2173)
                     /locus_tag="VFL49_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7912800.1"
                     /translation="MDRHPFVARLLQHYELSAADLKSFVAIIDGDLSIRRRHDLVVDG
                     VEYRKLCFVKDGYAVRYKLLRNGKRQIVNVVLPGDVVGLPGSFYERSAYSVTAVTELT
                     VNTCSLESYVQLCYKRPQFALALCWLAVREAANNAEQVITLGRRTPVERLSHFLLDVH
                     ARLRIVGRAEKESFALPFSQEIMADVLGLSVPHLNRMMQQLRSDKLIVDKDRHVTFTD
                     VDALKTLAHYQSQELTPIPGAMAPA"
     gene            complement(2427..3350)
                     /locus_tag="VFL49_12275"
     CDS             complement(2427..3350)
                     /locus_tag="VFL49_12275"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET7912801.1"
                     /translation="MRIAAMAAGAVGGYFGARLAAAGHDVFFIARGAHRDAIAKNGLK
                     IESMHGDLHLPKPNVTDDPSKVGPVDIVLFAVKLWDTETAAAAARPLLGPDSRLITFQ
                     NGIDSVERLSAILGADRVIGGAAYIATVIASPGVIKHSSGFAMMRFGRADKKPDNMLS
                     AFVDAGKAAKLDIALSADIQRELWQKFIFLTAMAGSTATLRSSIGPIVADTELRGFFR
                     ALMEEAFAVGKAKGVALDPAYLDDRMDFVVNKVEPGMKASMAHDLERGNRLELDWLAG
                     KVRALGRELRIPTPASDTVYTALKLHRMGSH"
     gene            complement(3499..4233)
                     /locus_tag="VFL49_12280"
     CDS             complement(3499..4233)
                     /locus_tag="VFL49_12280"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7912802.1"
                     /translation="MSKSVTFAAMAAAAALTSNLANAEPLFAFLTPPTPTQIAPQAVQ
                     PAPADEGDDAELPANLRRQIVGYSGREAPGTIIIDTPHTYLYYVLGGGKAVRYGIGVG
                     REGFTWSGVRSIERKAEWPDWYPPPEMLARQPYLPRMTAGGPGNPLGARAMYIAGTQY
                     RIHGTNAPSTIGKHVSSGCIRLTNEDVIDLYSRVQIGAKVIVLPQTSVAQSARPHPVS
                     ATQSYAAQPASPARATWDRIRRAGVY"
     gene            complement(4368..4865)
                     /gene="tsaA"
                     /locus_tag="VFL49_12285"
     CDS             complement(4368..4865)
                     /gene="tsaA"
                     /locus_tag="VFL49_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET7912803.1"
                     /translation="MASKDFGIRDGEKAVDLPSRPDADLYFIGRIHTPWKERKDCPKN
                     ARESEAVCLVEVDPRWREGLKDVETCSHLVLLYWMDRSPRNLVLQVPGHYGVQHGTFA
                     LRSPARPNPVAMSVVKLLRVDGNRLSVAGLDCLDGTPLLDIKPYFASTDSVPEAVVGW
                     HRQKD"
     gene            5013..6017
                     /locus_tag="VFL49_12290"
     CDS             5013..6017
                     /locus_tag="VFL49_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7912804.1"
                     /translation="MSGSGRLGFEALNSALKAAGEETRLRVLALLAEAELTVSDLTDI
                     LRQSQPRISRHLKLLVEAGLVERFREGTWAFFRLAEHGGGSDVARALLDKLNPADQTI
                     ARDRDRLASVRAARAAAAQTYFRRHAADWDRIRKLHVADAAVEEEIRAALADKPFRSL
                     LDLGTGTGRMLEMFGPDIERGLGLDLSLDMLLLARDRLERAGLKNCSVRQGDIYDLPL
                     ADDTFDVVILHQVLHFLDDGARAIREAARVLRPGGRLLVVDFAPHEQEFLREQFAHRR
                     LGFAPETVTQWITASGLEPAMHKSLAPEPDSEGKIAVSLWLARDTRALMATPLRREVA
                     "
     gene            6017..>6204
                     /locus_tag="VFL49_12295"
     CDS             6017..>6204
                     /locus_tag="VFL49_12295"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET7912805.1"
                     /translation="MNSPVRLSRFTPGAPRRIGVSFEFFPPNSEEMDRILWESIERLA
                     PLAPDFVSVTYGAGGSTR"
BASE COUNT         1060 a         1950 c         2017 g         1177 t
ORIGIN      
        1 ccgagccgtg cggccgcggc gtcatcgcgg ccgagccctg gccgcacgcg gtcgggcgcg
       61 agatcgctcc gcagcgtcgc cggatggtgc tggcggcctg cgtgcttgca tcgtcgatgg
      121 cattcatcga cggctcggcg ctgaccgtcg cgctgccgaa actgcgcgcc tatttcggcg
      181 ccgacctcgc ctcggtgcaa tgggttctca acggctatgt tctggtgctg gcggcgttga
      241 ccttgatcgg cggcgcgctc gccgatcact acggcaaggc gcggatgctg gccgtcgggt
      301 gcctcggctt cggcttcacc tcggtggcct gcgcgctggc cccatccgtg gaactgctgg
      361 tcgcgtgccg ggtcgcccag gggatcgcgg cggcgatcct cactccgtcc agtctcgcgc
      421 tgatcggcgc gacctaccca aaagaagagc gcaatcgtgc gatcggcgtg tgggcggcgg
      481 cctcggcgct gacgacagcc ggcggtccgg tgctcggcgg atggctcacc gagaacttcg
      541 gctggcaatg ggtgttcgcg atcaacccgc cgctggcgct gatcgccgtg gcgctgctga
      601 tgaatttcgc gccggccgac cagcatctgg cgcgccactt cgacatcgcg gggtcggcca
      661 ttctcgccgc cgcgctcgcc gcgctggcat gggcgctgag ccagatcggg cccggcgctg
      721 ccgcctcgaa cggcaccgcg gttgccgtcg tctcgatgct cggtctcggc gggctcgcct
      781 gctacgccgc atgggagcgg atcagcgcgc atccgatgac gccgccgcgg ctgacacgta
      841 accgtgcatt cgtggcgctc aacatcgcca cgctgctgat ctacaccggc ctggcgatca
      901 tgttctttgt gctgcccttt gatttcatcg accggcgcca cctctcgccg accgatgcgg
      961 gactggcttt ccttccgttc acgctcggcg tcgggttcct gtcgcaaccc ttcggcgcaa
     1021 tggcggacaa attcggtgcg cgcacgatgc tgatcgccgg gccctcggcc gcggcgctcg
     1081 ctctgctgct gctggcgctt ggtgaaaccg ctccgcttat acccggcgtg ctcctgccca
     1141 tgacgctgct gggtatttcg ttcgcgctgc tggtcgcgcc gctgacggcg tcggtcatgt
     1201 caagcgtgac ggatgcggaa gagggcctcg cttccggcgt gaacaacgcg atgagccggg
     1261 tcgcgcaact ctccggggtc gcgctttccg ccggcctggc atcttatgaa tccggctttc
     1321 gaaacggttt gatcgttgcc gccattgcca ccgccgcagg cgcattgacg attgctatat
     1381 tgctgccatc ggacggcggc aaagcgcgga gaacggcctg aaggattttg cgacgcgcgc
     1441 gctatgccgg ggccattgcc ccgggaattg gagtcagttc ctgggactga taatgcgcca
     1501 gagtcttgag ggcatcgaca tcggtgaacg tgacgtgccg gtccttgtcg acgatcagct
     1561 tgtccgaccg caattgctgc atcatgcgat tgaggtgcgg aacgctgagg ccgagaacgt
     1621 ccgccatgat ttcctgcgag aacggcagcg cgaagctttc cttctcggcg cggccgacaa
     1681 tacgaagccg cgcatgcacg tcgagcagga agtgcgacag gcgctcgacc ggtgtgcggc
     1741 ggccaagagt gatgacttgc tcggcattgt tcgccgcttc ccgcacggcc agccagcaca
     1801 gagccagcgc gaattgcggc cgcttgtagc aaagctgcac gtaggactcg agcgagcacg
     1861 tattgaccgt cagctcggtc acggcggtca ctgaataggc cgagcgctcg tagaagctgc
     1921 ccggcaggcc gaccacatcg cccggcagaa ccacattgac gatctggcgc ttgccgttgc
     1981 gcagaagctt gtaacgcacg gcatagccat ccttcacgaa gcaaagcttg cgatattcaa
     2041 ctccgtccac gacgagatca tgccgcctgc gaatcgacag atcaccgtcg atgatcgcga
     2101 caaaagattt cagatcggct gcactgagct cgtaatgctg caggagccga gcgacgaagg
     2161 gatgtcgatc caattttgcc ccgaacgtaa tgttgctgac gcttttcatc ggtcaaaggg
     2221 taatccgttt cattaatttt gatcaacaat gctggtcaag tcgccttggg tggaaacgtg
     2281 tcggttgcgg aaaattcccg ctctcggcaa tgagcgcgaa tgtcagctac ctgactttaa
     2341 aaatagccgc gcccgtgctg cggtcatttt tcgtgcgaaa cctgcgacaa atggtctgtg
     2401 cgatcagaac atcgcgcgaa catgcatcaa tgcgatccca tgcgatgcaa tttgagcgca
     2461 gtgtagaccg tgtcgctcgc aggcgtcggg atgcggagct cgcggccgag cgcgcgcact
     2521 tttcccgcaa gccagtcgag ctcaaggcga ttgccgcgtt cgagatcatg cgccatcgac
     2581 gccttcatcc cgggctcgac cttgttgacg acgaaatcca tgcggtcatc gagataggct
     2641 ggatcgagcg cgacaccttt ggccttgccg acggcgaaag cctcctccat cagcgcgcgg
     2701 aagaaaccgc gcagctctgt atcggcgacg atcggtccga tcgacgaccg cagggttgcc
     2761 gtcgatccgg ccatggccgt caggaagatg aatttctgcc acagctcacg ctggatgtcg
     2821 gccgacagcg ctatgtcaag ctttgccgcc ttgccggcgt cgacaaatgc actgagcatg
     2881 ttgtccggct ttttgtcggc gcgcccgaac cgcatcattg caaagccgct gctgtgcttg
     2941 atcacgcccg gagatgcaat cacggtcgct atgtaggcgg cgccaccgat aacgcgatcg
     3001 gcgccaagga tggcggacag ccgttcaacg ctgtcgatgc cgttctggaa agtgatgaga
     3061 cggctgtcag gccccagcag cggacgggcg gccgccgccg ccgtttcggt gtcccagagc
     3121 ttcaccgcga ataaaacgat gtcgaccggg cccaccttgg acggatcatc ggtcacgttc
     3181 ggctttggca aatggaggtc gccgtgcatg ctctcgatct tgagaccgtt cttggcgatc
     3241 gcatcgcggt gggcgccgcg cgcaatgaag aagacgtcgt gaccggccgc ggcgagccgg
     3301 gcaccgaaat atccgccgac tgcgccggcc gccatcgctg caatacgcat atgcttatcc
     3361 tttcagtctt cgctcatccc cgcgcacgcg ggaatgagcg ggcgtaaggt ttacccaagt
     3421 gatgcaaatg cagacaagca cacaaaaaat ttggcccggc gtgaggccgg gccagtgcgg
     3481 ggagaatagg ggaattgctc agtaaacgcc ggcacggcgg atccggtccc atgtcgcccg
     3541 tgcagggctg gccggctgcg ctgcatagga ctgggtcgca gacaccggat gcggacgggc
     3601 ggattgcgcg accgaggtct gcggcagcac gatgactttc gcgccgattt gcacgcggct
     3661 gtaaaggtcg atcacgtcct cattggtgag acggatgcag ccgctcgaaa cgtgcttgcc
     3721 gatggtgctc ggcgcattgg tcccgtgaat gcggtattgc gtgccggcaa tgtacatcgc
     3781 gcgcgcgccg agcggattgc ccgggccgcc tgcggtcatg cgcggcaggt agggctggcg
     3841 cgcgagcatc tcggggggcg gataccagtc cggccattcg gccttgcgct cgattgacct
     3901 cacgcccgac caggtgaagc cctcgcggcc gaccccgata ccgtagcgga ccgccttgcc
     3961 gccgccgagc acgtaataga gataagtgtg cggcgtatcg atgatgatgg tgccgggcgc
     4021 ttcgcggccc gagtagccga caatctgccg gcgaaggttc gccggaagct cggcgtcgtc
     4081 gccctcgtcg gccggtgcgg gttgcacggc ttgtggtgcg atttgtgtcg gggttggcgg
     4141 ggtgaggaag gcgaaaagcg gttcggcgtt cgcaaggtta gacgtcaacg cggcggcggc
     4201 tgccatcgcg gcaaacgtaa ctgacttgga catgacaccc tcgtcggtac accctcccgt
     4261 cttcggtcag gaaaatgacc gaagagttca aaggttccct gaagccgaag cggcaaaaac
     4321 gcttaaggct tgggaacgat ttgccgtaaa ttggattcgg aaattcttta gtctttttgc
     4381 ctgtgccaac cgacgaccgc ttccgggacg ctgtcggtcg aggcgaaata gggcttgata
     4441 tcgagcagcg gcgtgccatc caggcagtcg aggccggcga cggaaagccg gttgccgtcc
     4501 acgcgcagaa gcttcacgac gctcatggcg accgggttcg gccgggccgg cgagcggagc
     4561 gcgaatgtac cgtgctgaac gccatagtga ccgggcacct gcaggaccag gttacgcggg
     4621 gatcggtcca tccagtagag cagcacgagg tggctgcagg tttcgacatc tttgagaccc
     4681 tcgcgccaac gggggtcgac ctcgaccaga cagacggcct cggattcccg ggcattcttt
     4741 gggcagtcct tgcgctcctt ccacggcgtg tggatgcggc cgatgaagta gaggtcggcg
     4801 tccggccgag aaggcaggtc caccgccttt tcgccgtcgc ggattccaaa gtccttgctg
     4861 gccatctttc ctccttcggg gcggggagtg tgcccgggct ttataaacca cgccctaggg
     4921 acccactccg acgacttgct aggatctctt taaaggtata aagatatcct tatatggtgg
     4981 gaaccggccc aaaatcggtc gacaggcggt tcatgagtgg cagcggcagg ctcggtttcg
     5041 aggctcttaa ttcggctctg aaagcggccg gagaagaaac gcgcctgcgg gtgctggcgc
     5101 tcctcgcgga ggccgagctg accgtttccg acctcactga catcttgcgt caatcgcagc
     5161 cgcgcatctc gcgccacctc aagctgttgg tcgaggccgg gctggtcgag cgcttccgcg
     5221 aagggacctg ggcgttcttc cggcttgctg aacacggcgg cggctcggat gtcgcccgcg
     5281 cgctgctcga caagctcaat ccggccgacc agacgatcgc gcgcgaccgt gaccggctcg
     5341 catcggtgcg cgccgcgcgt gctgccgccg cgcagaccta tttccgcagg cacgcagccg
     5401 attgggatcg gatccgcaag ctgcacgtcg ccgacgcggc ggtggaggag gagatccgcg
     5461 cggcgcttgc cgacaagcct tttcgctcgc tgctcgatct cggcaccggc accggccgca
     5521 tgctcgagat gttcggcccg gatatcgagc gcggtctcgg cctcgatctc tcgctcgaca
     5581 tgctgttgct cgcgcgcgac cggctggagc gcgccggcct gaaaaactgc agcgtccggc
     5641 agggcgacat ctacgatctg ccgctcgccg acgatacgtt cgacgtcgtc atcctgcacc
     5701 aggtgctgca cttcctcgac gacggcgcgc gcgcaatccg cgaggcggcg cgggtgctgc
     5761 ggcccggcgg acgcctcctg gtggtcgact ttgcgccgca cgagcaggaa ttcctgcgcg
     5821 agcaattcgc gcaccgccgg ctcggctttg cgcccgagac ggtgacccag tggatcaccg
     5881 cgtccggtct cgagccggcg atgcacaagt cgcttgcgcc cgagccggac tccgaaggca
     5941 agatcgcggt ttcgctctgg ctcgcgcgcg acacacgagc gttaatggcc acccctctgc
     6001 ggcgcgaggt tgcgtaatga acagtcccgt gcgtttgagt cgtttcacgc ccggagcgcc
     6061 gcgccgcatt ggcgtgtcat tcgaattttt tccgccgaac agcgaagaga tggatcgaat
     6121 cctgtgggaa tcgatcgaac ggcttgctcc gcttgctccc gatttcgtgt ccgttaccta
     6181 cggcgccggc ggaagcacgc ggga
//
LOCUS       DASSRL010000604         6829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_925798, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000604 DASSRL010000000
VERSION     DASSRL010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925798"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..691)
                     /locus_tag="VFL49_12300"
     CDS             complement(68..691)
                     /locus_tag="VFL49_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET7912806.1"
                     /translation="MPNLTLYHASPSRSSIVLWMLEETGQPYDIKLIRLSAGDNLKPD
                     YLAVNPMGKVPALRHGDTVITEGAAICTYLADEFPAAKLNVPVGNTRRGDYLKWLFFG
                     PSVIEPAMTDRAFPRKESPRAAALGWRDFDTVLDIVSRAVAKGPHLMGEQFTAADVVI
                     GSGLRYGMMFKLIPERKEFQDYVARIAARPAAQRAQEKDKELSAQAA"
     gene            769..1050
                     /locus_tag="VFL49_12305"
     CDS             769..1050
                     /locus_tag="VFL49_12305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7912807.1"
                     /translation="MTMSNNVRDNSTLHRFELDADGHIAFSEYKRADGLLTIRHTVVP
                     KELEGRGIGSALIRGVLDTARAQGLKVKPLCPFAKAYIERHPEYADLQQ"
     gene            complement(1096..1998)
                     /locus_tag="VFL49_12310"
     CDS             complement(1096..1998)
                     /locus_tag="VFL49_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET7912808.1"
                     /translation="MRAMTGTITAPTTAAWQLWRDRRGRLSVLRIAVLALLLMPLAKA
                     VFEAGEIAHGARPLNELIHRAGFWALVFLGVTLAVTPFRRILRYGNLIEVRRMLGVGT
                     FCYIAAHLALFFADQSYSFARFFHEITHRVYLIIGAAAWLGLAALAATSTDGMVRRLG
                     GMRWRRLHQLIYVIVLFGLIHYFQQTKADVTVPTFAAGLFLWLIGYRVLAWRQKTSEL
                     STLSLLGLTLAVSLLTFAGEAIGIGIAFHVSPLRVLETVFDFDAGIRPGWQVLAAGLI
                     VVAIDAVMARVKQRMGRVREVAAE"
     gene            2298..2609
                     /locus_tag="VFL49_12315"
     CDS             2298..2609
                     /locus_tag="VFL49_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912809.1"
                     /translation="MSRALLGSLLVLVSALFAATNAFAHDSWINRGGFKNTAGEWCCG
                     DYDCKSYTRTSSTASGWMVDGELVPFDEAMPVAPPDGMLTICRRPDGTRRCVFGLKPG
                     L"
     gene            complement(2801..3049)
                     /locus_tag="VFL49_12320"
     CDS             complement(2801..3049)
                     /locus_tag="VFL49_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912810.1"
                     /translation="MPIHLPPKARVRALRARKQTEIGSIDWLGLWALSLAFCAINLAA
                     IAYFVNGDNTGASCILVGAAALIAVIVRFAGEHGEQSD"
     gene            complement(3182..3637)
                     /gene="croR"
                     /locus_tag="VFL49_12325"
     CDS             complement(3182..3637)
                     /gene="croR"
                     /locus_tag="VFL49_12325"
                     /EC_number="4.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HET7912811.1"
                     /translation="MLPLQMLYFEDLSVGMTETLRKTIDSSDVVGFAEVTGDRNPIHL
                     SEHFAAKTPFGTRIAHGLYTASLISAVLGTRLPGPGAVYISQTLNFRAPVKIGDTVDV
                     SVEVAELIPEKSRARLACRCSVDDEIVLDGEAWVKVPSKQATGRPLPRI"
     gene            complement(3670..4878)
                     /locus_tag="VFL49_12330"
     CDS             complement(3670..4878)
                     /locus_tag="VFL49_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912812.1"
                     /translation="MPSPSQQSLDRRIVITALGLGQILAWGTSFYFPAVFAGPIVAET
                     GWSLGYVVAGTSLGLLVAGLISPQVGRIIDRRGGRPVLLASSLFYAAGLAGIGASPAL
                     PVYLGSWALIGLGMGTGLYDAVFAALGRMYGSEARTPITNLTLFGGFASTICWPLSAF
                     MIEHIGWRPACFVYAGLHLLLVLPLQMTVVRPVTPGAGSAAASEKQAGNKASPIRNET
                     LIFALLAAVLSMAAGVGSIVVVHLLIFLQARGVDFAAAVTLGTLFGPAQVGARIVERL
                     FGSRYHPVWTMVASCALMAAGLLMLYGALPALLIVILLYGAGYGISWIGRGTLPLALF
                     GPARFPRLMGKLAFPSLIIQALAPSAGALLIEARGVNATIGILTLLALINVLLIGVLW
                     WLCRKPGTDE"
     gene            5187..5342
                     /locus_tag="VFL49_12335"
     CDS             5187..5342
                     /locus_tag="VFL49_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HET7912813.1"
                     /translation="MIAAIVRFIQEWKRYNRSLNELSRLGDRELADIGISRSDIPRVA
                     WNAAHRS"
     gene            complement(5417..6040)
                     /locus_tag="VFL49_12340"
     CDS             complement(5417..6040)
                     /locus_tag="VFL49_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912814.1"
                     /translation="MRLNATLYVAGCVAVVASASIACGATRPGVVAATFTEYSVSPPT
                     PDTVFVCHGFGCKYRAEIDLTPADRAKLAQFLAAGRASAAAERNAVAAAGAWFDKRVG
                     PVAGTTNHVARAGMKYMFDTHQFDCIDSSRNTTSLLMVLDQLNLLRHHDVDEPEARGY
                     MVDGRPPHATAVLVERATGKKWSIDSWTVGYGQALEVMPLDRWKALD"
     gene            6096..>6829
                     /gene="trmFO"
                     /locus_tag="VFL49_12345"
     CDS             6096..>6829
                     /gene="trmFO"
                     /locus_tag="VFL49_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET7912815.1"
                     /translation="MTARQIKAVHVIGGGLAGSEAAWQVARADVPVVLHEMRPQQTTP
                     VHKTGRCAELVCSNSFRSDDAEHNAVGLLHEEMRRLGSLIMRAADANQVPAGGALAVD
                     RDGFAEAVSAAISAHPLIELQRGEITSPPDDWDSVIIATGPLTSPGLATAIGTLTGEQ
                     QLAFFDAIAPIVHRDSIDLGQAWFQSRYDKAGPGGSGADYINCPLSREQYETFIAALL
                     GGDKTAFHEWEASTPYFDGCLPIEVMA"
BASE COUNT         1383 a         2164 c         2133 g         1149 t
ORIGIN      
        1 tgatcgtgtg gggattggtc gcttcggttg ttcgctaccg aaaacaggtc taaccagcag
       61 gctgttgcta agccgcctgc gccgacaatt ccttgtcctt ctcctgtgcg cgctgcgcgg
      121 ccggacgcgc ggcgatgcgc gcaacgtagt cctgaaactc cttccgctcg gggatcagct
      181 tgaacatcat gccgtagcgc aggccggagc cgatgacgac gtcggcggcg gtgaactgct
      241 cgcccatcag atgcgggcct ttggcaaccg ccctgctcac gatgtcgagc acggtgtcga
      301 agtcgcgcca gccgagcgcg gcggcgcgcg ggctctcctt gcgcgggaag gcgcggtcgg
      361 tcatcgccgg ctcgatcacg ctcggtccga agaacagcca cttgagatag tcgccgcgcc
      421 gcgtgttgcc gacggggacg ttgagcttcg cggccggaaa ttcgtcggcg agataagtgc
      481 agatcgccgc gccttcggtg atcaccgtat cgccatgccg cagcgccggc accttgccca
      541 tcgggttgac cgcgagatag tcgggcttga gattgtcgcc ggcgctcagc cggatgagct
      601 tgatgtcgta aggctgtccg gtctcctcca gcatccagag cacgatggag gagcgcgacg
      661 gcgaggcatg gtagagcgtc agattcggca tgaagcacct tgcaaaattg gcggggacaa
      721 tcgacctaca gctggatcat caatgtgcag agaccgtgac caaaggccat gaccatgtcc
      781 aataacgttc gcgacaacag cacgcttcac cgcttcgagc tcgatgccga cggccacatc
      841 gccttttcgg aatacaagcg cgccgacggc ttgctcacca tccggcatac cgtggtaccc
      901 aaagaactcg aaggccgcgg catcggctcg gcgctgatcc gcggcgtgct cgacacggcg
      961 cgcgcgcaag gcctgaaggt gaagccgctg tgccccttcg ccaaggccta tatcgaacgt
     1021 caccccgaat atgcggacct gcagcaatag gtcatgcgca agcgcgggat ttccgtcacc
     1081 ggcgacacac agacatcatt cggcagcgac ctcgcgcacg cgacccatgc gctgtttcac
     1141 gcgcgccatc acggcgtcga tcgcaacaac gatcaggccg gcggcaagca cctgccatcc
     1201 cggcctgatg ccggcatcga aatcgaatac cgtctcgagc acgcgcagcg gcgaaacatg
     1261 aaatgcaatg ccgatgccga tcgcctcgcc ggcgaaggtg aggagcgaga cggcgagcgt
     1321 gaggccgagc aggctcagag tcgaaagctc gcttgtcttt tgtctccagg cgagcacccg
     1381 gtagccgatc agccagagga acaggccggc cgcgaaggtc ggcacggtta cgtcggcctt
     1441 ggtctgctgg aaataatgaa tgagaccgaa cagcacgatc acatagatca gctggtgcag
     1501 gcggcgccat cgcatgccgc cgagccggcg caccatgccg tcggtcgacg tcgcagccag
     1561 cgccgcaagc ccgagccatg cggccgcgcc gatgatcaga tagacgcggt gcgtgatctc
     1621 gtgaaaaaac ctggcgaagc tgtagctctg gtcggcgaaa aacaacgcga ggtgcgcggc
     1681 gatgtagcag aaggtgccga cgccgagcat gcggcggacc tcgatcagat tgccgtagcg
     1741 caagatgcgg cggaacggcg tcaccgccag cgtcacgccg agaaaaacga gcgcccagaa
     1801 gccggcacga tgaataagct cgttgagcgg gcgcgcgccg tgcgcgatct cacccgcctc
     1861 gaataccgcc ttggcgagcg gcatcagcag gagcgcaagc acggcgatgc gcaggaccga
     1921 cagacggccg cggcggtcgc gccacaattg ccaggccgcg gttgttggag cggttattgt
     1981 gccggtcatc gcgcgcatat cagacacgcg ttgccaaaac ggcgcaaatc catcggaaat
     2041 ttatgccgtt tcatggttcc ctcgaattat cccgccgttt cccgcccgct atcacgacga
     2101 gcaattaacc catcgaatcg cgcgatcgtg ttaccatttt catgtcgcct tgattcgtca
     2161 cgtataatca caagcttgcg tgcaaacgtt acgccgcaac ggaacaaaat cacgcggcca
     2221 acaattacgg aatgcggagg aggagcagta gcagaccaca gtgaagctgc gccgggggag
     2281 tttgatggag gccaccgatg tcgcgagctt tgctcggctc actgcttgtt ctcgtgtccg
     2341 cgcttttcgc cgccactaac gccttcgcac acgacagctg gatcaatcgc ggcggcttca
     2401 agaacaccgc cggcgaatgg tgctgcggcg attacgactg caagagctac acgcgcacgt
     2461 catcgaccgc gtcggggtgg atggtcgacg gcgagcttgt gccgttcgac gaggccatgc
     2521 cggtcgcgcc gccggacggc atgctcacga tctgccgccg gcccgacgga acgcggcgct
     2581 gcgtctttgg tctcaagccc ggtttgtagc agcgcacctg taaccggaag gcaatcggtc
     2641 tcaacctccg ctcattcccg cgcaggcggg aagccggcaa ttttgattcc gggtccccgc
     2701 tttccgcctg cgcgctccgc gcttcggcgg actcaaaccc gccgacgcgc aaagcgagcg
     2761 acggttgggt cgcggggacg agcggataat gggtttgtgt ctaatcagat tgttcgccgt
     2821 gctcgccggc gaagcgaacg atcaccgcga tcaacgcggc ggcgccgaca agaatacagg
     2881 acgcgccggt gttgtcgccg ttcacgaaat aggcgatggc ggcaagattg atcgcacaga
     2941 acgcaagcga cagcgcccag agtccgagcc agtcgatcga gccgatctca gtctgcttgc
     3001 gcgcacgcag cgcacggacg cgggctttcg ggggcagatg aataggcatg cgaccgtctc
     3061 cacttttgct gtcaccctcc cctttgctct tacgagcgaa ttccgtgcct gcattgatgt
     3121 aacgcaagtt ttctttgcaa gttccttgca aaacacgcgg caaattgagc cgattattga
     3181 ttcaaatgcg cggcagcgga cgtcccgtcg cctgcttcga aggaactttc acccaggctt
     3241 cgccgtcgag cacgatctcg tcgtccaccg agcagcggca ggcgagccgc gcgcgcgact
     3301 tttccggaat gagctctgcc acttcgacgc tgacatcgac ggtgtcgccg attttcaccg
     3361 gcgcgcggaa attcaaggtt tgcgaaatgt aaaccgcgcc gggaccgggc agccgcgtgc
     3421 cgagcacggc ggaaatcagg ctggccgtat agaggccatg ggcaatgcgc gtgccgaagg
     3481 gtgtcttggc cgcgaaatgc tcggacagat gaatcggatt gcggtcgccg gtgacttcgg
     3541 cgaagccgac cacgtccgag gaatcgatgg tcttcctgag ggtctcggtc atgcccaccg
     3601 agagatcctc gaaatacagc atctgcagcg gcaacatctt acggctctgg gtttgggcaa
     3661 gggtaacgct tattcatcag tacccggttt gcggcagagc caccacagca cgccgatcaa
     3721 cagcacgttg atcagcgcga gcagcgtcag gatgccgatg gtggcgttca cgccgcgcgc
     3781 ctcaatcagc aacgccccgg cagatggcgc cagcgcctgg atgatcaagc tcggaaaagc
     3841 gagcttgccc atcagccgcg gaaaccgcgc cggtccgaac agcgcgagcg gcagcgtgcc
     3901 gcggccgatc caggaaattc cgtacccggc cccgtacagc aaaatgacga tcagcagagc
     3961 gggcagcgcg ccgtacagca tcagcaggcc ggcggccatc agcgcgcagg acgcaaccat
     4021 ggtccagacc ggatgatagc ggcttccgaa caggcgctcg acgatgcgcg cgccgacctg
     4081 cgccgggccg aacagcgtgc cgagcgtgac ggctgccgca aaatccacgc ccctcgcctg
     4141 caagaaaatc agcagatgca cgacaacgat cgagccgacg cccgcggcca tcgacagcac
     4201 ggcggcgagc agcgcgaaaa tcagcgtttc gttgcggatc ggcgacgcct tattgccggc
     4261 ctgcttttcg cttgcggcgg ccgatcccgc gccgggcgtc accggacgca cgaccgtcat
     4321 ctgcagcggc agaacgagca aaagatgcag gcctgcgtaa acaaagcacg ctggacgcca
     4381 gccgatatgc tcgatcatga aggcgctgag cggccagcag attgtgctgg caaagccgcc
     4441 gaacagcgtg agattggtga tcggtgtgcg cgcctcgctg ccgtacatgc ggccgagcgc
     4501 ggcaaagacc gcgtcgtaca ggccggtgcc catgccgagg ccgatcagcg cccacgaacc
     4561 cagatacacc ggcaatgccg gtgaagcgcc gatgccggca agacctgcgg catagaaaag
     4621 agagctggcg agcaagaccg gccggccccc gcgccggtcg atgatgcggc cgacctgcgg
     4681 ggaaatcagg ccggcaacca aaaggccgag cgacgtgccg gcgacgacat agccgagcga
     4741 ccagccggtc tcggcgacga tcggccctgc aaataccgcg ggaaaataga aagaggtgcc
     4801 ccacgccaga atctggccaa ggccgagcgc cgttatgacg atgcggcgat cgagggattg
     4861 ctgcgatggc gatggcaaga ttcgccggtc ctcagctcgc gggcccgcat tcggacgtca
     4921 tcaccgcctc cacgaatcta tgcgcaggca atctacacaa atgcccgcca ttggcacctt
     4981 ttgtgcgctg cagcgcgctt ttcgtcgtgt tgccccggct ttcggaggta aaagatgaat
     5041 tattccttta aaaactgtaa gttaagctat gcatttttca cgtagctgcg gcgcaacaat
     5101 gttgcttttc atgggaccga acgtgcctat gttcctagcg aacaatcgac cggcacagag
     5161 ccggacccaa ggaaagaagg actgtcatga ttgccgcaat cgtccgcttc atccaggagt
     5221 ggaagcgtta caaccggagc ctcaacgagc tgtcgcgcct gggcgaccgc gagctggccg
     5281 atatcggcat cagccgctcg gacattccgc gcgttgcctg gaacgctgcg caccgcagct
     5341 aatcctccaa tacgcgctgt tatccggaaa acgcccgcgg gtccccgcgg gcgtttcgtt
     5401 tgcgcgcagg ccggagctaa tcgagcgcct tccagcgatc caaaggcatg acttcgagcg
     5461 cctggccata gccgacagtc caggagtcga tcgaccactt cttcccggtt gcccgctcca
     5521 ccagcaccgc agttgcatgc ggcggccggc cgtcgaccat gtaaccgcgt gcttccggtt
     5581 catcgacgtc gtgatggcga agcaaattga gctgatcgag caccatcagc aggctcgtgg
     5641 tgttgcggct gctgtcgatg caatcgaact ggtgcgtgtc gaacatgtat ttcatgccgg
     5701 cgcgggcgac gtgattggtc gtgccggcga ccgggccgac ccgcttgtcg aaccaggcgc
     5761 ctgcggccgc gacggcattg cgttcggctg cggccgaagc gcgtcccgcc gccagaaact
     5821 gcgcgagctt ggcgcggtca gccggggtca gatcgatttc cgcgcgatac ttgcacccga
     5881 agccgtggca gacaaaaacg gtatcggggg tcggcggaga aaccgaatac tcggtaaagg
     5941 tcgctgccac cacgccgggc cgtgtcgctc cgcatgcgat cgaagcactc gccacaaccg
     6001 caacacagcc ggcaacgtaa agcgttgcat tcaaacgcat ccggaacccc ttaccgccgt
     6061 tgcggcttgc cgcccatgag cctatgcatc atcgaatgac agcgcgtcaa ataaaagccg
     6121 ttcacgttat cggcggcggc ctcgcgggct cggaggccgc gtggcaggtt gcgcgggcgg
     6181 atgtgcctgt cgtgctgcac gaaatgcggc cgcaacagac cacgccggtg cacaagaccg
     6241 gccgctgtgc cgagctcgtc tgctcgaatt cattccgctc cgatgacgcg gagcacaatg
     6301 cggttggcct gctgcacgag gaaatgcgcc gcctcggctc gctcatcatg cgtgccgccg
     6361 acgcaaacca ggtgccggcc gggggcgccc tggcggttga ccgtgacggg tttgcagaag
     6421 ccgtctcggc ggcgatttcc gctcatcctt tgattgaact gcagcgcgga gaaatcacct
     6481 caccgccgga cgactgggac agcgtcatca tcgcgaccgg accgctgacc tcgcccggcc
     6541 ttgcaacggc aatcggcaca ttgacaggcg agcagcagct cgcgtttttc gacgcgatcg
     6601 cgccgatcgt gcatcgagac agcatcgacc tcgggcaagc ctggttccag tcgcgctacg
     6661 acaaggccgg cccgggcgga tcgggagccg actacatcaa ttgcccgctg tcccgcgaac
     6721 aatacgagac atttatcgcg gccctgcttg gcggggacaa aaccgcgttt catgagtggg
     6781 aggcttcgac accctatttc gacgggtgcc tgccgatcga ggtgatggc
//
LOCUS       DASSRL010000605         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1345629, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000605 DASSRL010000000
VERSION     DASSRL010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345629"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /gene="bcsS"
                     /locus_tag="VFL49_12350"
     CDS             complement(<1..213)
                     /gene="bcsS"
                     /locus_tag="VFL49_12350"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HET7912816.1"
                     /translation="MPPDPAHADDADDAQIMLFSGRDIWRSGAFAYGGFMFAPGGFDQ
                     DGILLKIMLSGGLYRYNAQNLGGAQVT"
     gene            442..1674
                     /locus_tag="VFL49_12355"
     CDS             442..1674
                     /locus_tag="VFL49_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912817.1"
                     /translation="MSAASQARHASDDRARRRSIAFLNWAHTIDHFVLLIYPTVVIGL
                     QAVYQRSYSELIALSSTAFIAFGVFSLPAGWLADRWSRRNMMTAFYIGCGLSLAAAGL
                     APNLTTLAVAMFALGMFASIYHPVGMAMLIEASNARGRTLAFNGVCGNLGVSLAAGIS
                     AALATWLGWRAAFYVPAALCIVTGIVYFWVTPDDLHHVKSRVSSPAVPLTPRLAVMLF
                     GLFIVIALSAGLTFNVLTIALPKIVDERLSRHLPLLLVGSIATAVLVCGALAQLMVGR
                     LVEWVPPHVIFAIVTGVGFLGNLWAANAGGIALLVALAIAVAAIYGQVTVNDMIMARY
                     TADAWRGRVYAVRYFLLFISAGAAIGMISLLHETGGFALVLGVNAVIALLLFITTLAL
                     VAMIISVEGRQRKPQPAE"
     gene            complement(1676..>2006)
                     /locus_tag="VFL49_12360"
     CDS             complement(1676..>2006)
                     /locus_tag="VFL49_12360"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7912818.1"
                     /translation="GFSKRQLASALEHEGIGYVHLQKLGTPAEGRAAARDGDTDALWR
                     IYDKHIGGADAQNELRELIALIKSGKRIALLCYCRDPKTCHRSRIVANVRKKMAVQVT
                     DLIPPLF"
BASE COUNT          360 a          624 c          607 g          415 t
ORIGIN      
        1 ggtgacctgt gcgccgccga gattttgggc gttgtagcgg tagagcccgc ccgacagcat
       61 gatcttcaga agaataccgt cctggtcgaa gccgccaggc gcaaacatga agccgccata
      121 ggcaaaggcg ccgctgcgcc agatgtcgcg gcctgaaaaa agcatgattt gcgcgtcatc
      181 ggcgtcgtcg gcatgcgcag gatcaggcgg cacacgaaga cagcaccaga taaaagcggc
      241 gacgaaacag gccgccgtcc aaacgcgcct ccgacaactc acggaacaaa tcccggatcg
      301 ctacaaaata tcgctgcaat ccaaggctaa ataatcttat cgctacaacc aacagtcgaa
      361 cggaggagag ccgttaggac aaagctctat caatgggtat atgcatgcta ccgaatccct
      421 tgtgccgaat ggaaatccgg aatgagtgcc gcatcccaag cccgccatgc ttctgacgat
      481 cgagcgcgcc ggcggtcgat cgcgttttta aattgggcgc acacgatcga ccatttcgtg
      541 ctgctgatct acccgacggt ggtgatcggg cttcaggcgg tctaccagcg ctcctacagc
      601 gaactgatcg cgctttcgag cacggctttc attgccttcg gcgtgttctc gcttccggcc
      661 ggatggcttg ccgaccgctg gagccgccgc aacatgatga cggccttcta tatcggttgc
      721 ggactctcgc tggcggctgc cggactcgca cccaatctta cgacgctggc ggtcgccatg
      781 tttgcgctcg gcatgtttgc atcgatctat caccctgtcg gcatggcgat gctgatcgaa
      841 gcgtcgaacg cgcgcggacg cacgctggcc ttcaacggcg tatgcggcaa tctcggggtc
      901 tccctcgctg ccggcatcag cgcggcgctc gccacctggc tgggctggcg cgcggcattc
      961 tacgtgcctg ccgcgttgtg tatcgtcacg ggcatcgtct acttctgggt cacgcctgac
     1021 gaccttcacc acgtcaagtc gcgcgtatcc tcgccggcgg ttccgctcac gccgcgcctc
     1081 gcggtgatgc tgttcgggct gttcatcgtg atcgcgctca gcgcgggact gacgttcaac
     1141 gtcctcacca ttgcgctgcc gaaaatcgtc gacgaacgcc tgtccaggca tctgccgctc
     1201 cttctcgtcg gcagcatcgc gacggccgtg ctggtctgcg gggcattggc gcaactgatg
     1261 gtcggccggc tggtcgaatg ggttccaccg catgtgatat ttgcgatcgt caccggtgtc
     1321 ggcttcctcg gcaatctgtg ggccgccaat gccggcggca tcgcgcttct ggtcgcgctc
     1381 gcgatcgcag tggctgcgat ctacggacag gttacggtca acgacatgat catggcgcga
     1441 tacacggccg atgcttggcg cggccgcgtc tacgcggtgc gctattttct gttgttcatc
     1501 agcgccggcg ccgcgatagg aatgatatcg ctgctgcacg agacgggagg ctttgcgctc
     1561 gtattggggg tgaacgccgt catcgctctg ctcttgttca tcacaacgct ggctctcgtt
     1621 gcaatgatca tcagcgtcga aggcaggcag cgcaagccgc agccggccga atagttcaga
     1681 acagcggcgg aatgaggtcg gtgacctgca ccgccatttt cttccgaacg ttcgcaacaa
     1741 tacggctgcg atggcaggtc ttcggatcgc ggcagtagca gagcagcgcg atccgcttgc
     1801 ctgatttgat caatgcaatc agctcgcgca attcgttttg cgcatcggcg cccccgatgt
     1861 gcttgtcgta aatgcgccag agcgcatcgg tgtcgccgtc gcgtgcggcc gcgcgacctt
     1921 ccgccggcgt gccgagcttc tgcaggtgca cgtagccgat gccttcgtgc tccagcgccg
     1981 atgcgagttg acgcttggag aagccg
//
LOCUS       DASSRL010000606         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1275830, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000606 DASSRL010000000
VERSION     DASSRL010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275830"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFL49_12365"
     CDS             complement(<1..423)
                     /locus_tag="VFL49_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7912819.1"
                     /translation="MAKEKFQRNKPHCNIGTIGHVDHGKTTLTAAITKVLAEKGGATF
                     TAYDQIDKAPEEKARGITISTAHVEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVYMNKVDMV"
     gene            complement(463..2538)
                     /gene="fusA"
                     /locus_tag="VFL49_12370"
     CDS             complement(463..2538)
                     /gene="fusA"
                     /locus_tag="VFL49_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7912820.1"
                     /translation="MPRSHPIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVH
                     EGAATMDWMEQEQERGITITSAATTAFWKEKRLNIIDTPGHVDFTIEVERSLRVLDGA
                     VCVLDSNQGVEPQTETVWRQGDKYKVPRIVFANKMDKIGADFFKCLDDIKTRLGAKPV
                     AIQLPIGSEQNFKGLVDLVRMKGVVWDDESLGAKYHDIEIPADMLDEAKKYREQMVEA
                     AVELDDAAMTAYLEGKEPDEATLKKLIRKAVITSAFFPVLCGSAFKNKGVQPLLDAVV
                     DYLPSPLDVPPIKGVNPDSGEEVVREPKDDAPMALLAFKIMDDPFVGTITFCRIYSGI
                     LASSTGVINSTKERKERIGRMLLMHANNREDIKEAYAGDIVALAGLKEVRTGDTLCDP
                     AKPVILEKMEFPDPVIEIAIEPKSKADQEKLGVALAKLAAEDPSFRVSTDQESGQTIL
                     KGMGELHLDIKVDILKRTYKVDANIGAPQVAFREKITRRVEHGYTHKKQTGGSGQFAQ
                     VKIIAEPTPPGTPFEFENEIVGGAVPKEFVPGVEKGLESVLGSGVVAGFPVVDLKVTL
                     IDGKYHDVDSSALAFEICARQCLKEALQMAKPVLLEPIMKVEVVTPEDYTGSVIGDLN
                     SRRGQIQGQDMRGNANVINAMVPLMNMFGYVNNLRSMSQGRATFTMQFDHYAELPANV
                     SAEVQKKFA"
     gene            complement(2569..3039)
                     /gene="rpsG"
                     /locus_tag="VFL49_12375"
     CDS             complement(2569..3039)
                     /gene="rpsG"
                     /locus_tag="VFL49_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7912821.1"
                     /translation="MSRRHAAEKRDIIPDPKFGNIVVSKFMNAIMHDGKKSVAEGIVY
                     GALETIESKTKQNPITVFQQALDNVMPSIEVRSRRVGGATYQVPVEVRTSRRQALGIR
                     WIIAAARERNERTMTERLSAELLDASNNRGNAVKKREDTHKMAEANRAFSHYRW"
     gene            complement(3206..3577)
                     /gene="rpsL"
                     /locus_tag="VFL49_12380"
     CDS             complement(3206..3577)
                     /gene="rpsL"
                     /locus_tag="VFL49_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7912822.1"
                     /translation="MPTINQLIAKPRHPLKARNTVPALQASPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIPGEGHNLQEHSVVMIRGGRVKDLPGVRYHILRGVL
                     DTQGVKNRKQRRSKYGAKRPK"
BASE COUNT          704 a         1313 c         1261 g          960 t
ORIGIN      
        1 gaccatgtcg accttgttca tgtagacgac gagcgccgga acaccgacct gccgggcaag
       61 cagaatgtgc tcgcgggtct gcggcatggg gccgtcggca gcggacacca ccagaatagc
      121 gccgtccatc tgcgcggcgc cggtgatcat gttcttcacg tagtcggcat ggcccggaca
      181 gtcgacgtgc gcgtagtggc gcttgtcggt ctgatactcg acgtgcgcgg tcgagatggt
      241 gatgccgcgc gccttctctt ccggggcctt gtcgatctgg tcgtaggccg tgaacgtagc
      301 gccgcccttc tcggccagga ccttggtgat cgccgccgtc agcgtggtct tgccgtggtc
      361 gacgtgaccg atcgtcccga tattgcaatg gggcttattt cgctggaatt tctctttggc
      421 catggaactc tccgttcaga tccttctgtt atctttttcc cgtcacgcga atttcttctg
      481 cacttccgct gacacgttcg cgggcagctc ggcgtagtga tcaaattgca tcgtgaacgt
      541 ggcgcggccc tgagacatgg accgcagatt gttgacgtag ccgaacatgt tcatcagcgg
      601 caccattgcg ttgatcacgt tggcattgcc gcgcatatcc tggccctgaa tctggccgcg
      661 ccgtgaattg agatcgccaa tgacggagcc ggtatagtct tccggcgtca ccacctcgac
      721 cttcattatc ggctcaagca gaaccggctt ggccatctgc agcgcctcct tcaggcactg
      781 gcgcgcgcag atttcgaagg cgagcgcgga cgagtcgacg tcgtgatatt taccgtcgat
      841 caatgtcacc ttgagatcca ccaccgggaa tcccgccaca acgcccgagc cgagcaccga
      901 ctccaaaccc ttctcgacgc cggggacaaa ttccttgggt accgcgccgc cgacgatctc
      961 gttttcgaat tcgaacggcg tgccgggcgg cgtgggctcg gcaatgatct tgacttgcgc
     1021 gaactgaccg gaaccgccgg tctgcttctt gtgggtgtag ccgtgctcga cgcgccgggt
     1081 gatcttttcg cggaaggcca cctggggcgc gccgatattg gcgtcgacct tgtaggtccg
     1141 cttcaagata tcgaccttga tgtcgaggtg cagttcgccc attcccttga gaatggtctg
     1201 cccgctctcc tggtcggtcg aaacgcggaa tgacggatct tcggccgcga gcttggcaag
     1261 tgcaacgccg agcttttcct ggtccgcttt cgacttgggc tcgatcgcga tctcgatgac
     1321 gggatcggga aattccatct tttccaggat caccggcttc gcggggtcgc acagcgtgtc
     1381 accggtgcgg acctccttca ggcctgccag cgcaacgatg tcgccggcat aggcctcctt
     1441 gatgtcttca cggttgttcg catgcatcag cagcatgcgg ccgatacgct ctttgcgctc
     1501 cttggtcgaa ttgatgacgc cggtactgct cgcaaggatg cccgaataga tgcggcagaa
     1561 ggtgatggtg ccgacgaaag ggtcgtccat gatcttgaag gcaagaagcg ccatcggcgc
     1621 atcgtctttt ggctcgcgta caacttcttc gccgctgtcg ggattgacgc ccttgatcgg
     1681 cggcacgtcg agcgggctcg gcagatagtc gacgaccgca tcgagcagcg gctgaacgcc
     1741 cttgttcttg aacgccgagc cgcacagcac cgggaagaat gcgctggtga taacggcctt
     1801 gcggatcagc tttttgaggg tcgcctcgtc gggctccttg ccctcgaggt aggcggtcat
     1861 ggctgcgtcg tcgagttcaa ccgccgcttc gaccatttgc tcgcgatact tcttggcctc
     1921 gtcgagcatg tcggccggga tctcgatgtc gtgatacttg gcaccgagcg actcgtcgtc
     1981 ccacaccacg cctttcatgc ggaccaggtc gacaaggcct ttgaagttct gttccgaacc
     2041 gatcggaagc tggatagcca ccggcttcgc gccgaggcgg gtcttgatgt catcgaggca
     2101 cttgaagaag tcggcaccga tcttgtccat cttgttggca aagacaatgc gcggaacctt
     2161 gtacttgtcg ccctgacgcc agactgtctc ggtctgcggc tccacgccct ggttggaatc
     2221 cagcacgcat accgcgccgt cgagcacgcg cagcgaacgc tcaacctcaa tagtgaagtc
     2281 gacatgtccg ggtgtgtcga tgatattgag gcgcttttct ttccagaagg ctgtcgttgc
     2341 ggccgacgtg atcgtgatgc cgcgctcctg ctcctgctcc atccaatcca tcgtcgcggc
     2401 gccttcgtgc acctcgccga tcttgtggct cttgccggtg tagtagagga tccgctcggt
     2461 cgtcgtcgtc tttccggcat cgatgtgcgc catgatgccg aagttgcggt agtcctcgat
     2521 tgggtggctg cggggcatag gggatcagtc cttaaatctc ttctgctgtc accagcggta
     2581 atgcgagaag gcccggttgg cttccgccat cttatgggtg tcctcgcgct tcttgactgc
     2641 gtttcccctg ttattggatg cgtcgagcag ctcagcggag agccgctcgg tcatcgtgcg
     2701 ttcgttgcgc tcgcgcgcgg ccgcgataat ccagcggatg ccgagcgcct gccggcgtga
     2761 cgtgcgcact tcgaccggca cttggtacgt ggcgccgccc acgcggcgcg agcgcacctc
     2821 gatcgaaggc atcacgttgt cgagggcctg ctggaagacc gtaatcgggt tctgcttggt
     2881 cttgctctcg attgtctcaa gcgcgccata gacgatgcct tccgccaccg acttcttgcc
     2941 gtcatgcatg atggcattca tgaatttgct gacgacaatg tttccgaact tcggatccgg
     3001 gatgatgtcg cgtttttcgg cagcgtgacg gcgagacatg attttccagt cctaagctct
     3061 cgatctcttt tcttgtcatg gccgggcttg tcccggccat ccacgtctta atctcacttc
     3121 attgccggcg aaccggcatc aacttgcctc tcgcgttccc cgggtctcgc ttcgctcgtc
     3181 cggggatctc cgcaaagagg ccgaatcatt tcggcctctt tgctccgtac ttcgaacggc
     3241 gctgtttgcg gttcttcacg ccttgcgtgt cgagcacacc gcgcagaatg tgatagcgca
     3301 cgcctggcag gtccttcacc cggccgccac ggatcatcac gaccgagtgc tcttgcaggt
     3361 tatgaccctc gcccgggatg tagccgatca cctcaaagcc gttggtgagc cgcaccttcg
     3421 ccactttccg cagagccgag ttcggcttct ttggtgtcgt cgtgtagacg cgcgtgcaga
     3481 ccccgcgctt ctgcggggac gcctgcaggg ccggcaccgt attgcgcgcc ttgagcgggt
     3541 gccgcggctt ggcaatcagc tggttgatcg tcggcatcgt tgccttcact accttcagtt
     3601 tggggatgcc gcgcgaaccg aacgacccgc agcgcccctc gttaatttcg tgcggacatc
     3661 acccgcgtcc gcgccgcgtg gggctcagcg ccccggcatt ggcgtgcgcc gggcaacggc
     3721 gcacgcaggc tcgcgcgact ttcccacgcg caaaacgaaa tcgcgccatc cgccaaccgc
     3781 ggcagggcgc tcttccgttc ggacggacgc gttgcgctta agcaccgcgc ccgttcattc
     3841 ccaagtctga caggtgctgt cagaggcagc gtttgagctt tattcgtcga ggcgtagtgc
     3901 ccaacgccgt tcgagaaaaa acagcattct caaatggtta gtcgggtggc cgttccgagc
     3961 aagcaaagct caccgcctgc cgaaaagtgt gcggtatgta tcggcgcgac tccgtcaagt
     4021 caagcagaaa tggctgaaaa accgggcatt cgggaccttc gagagccgaa tacctaatga
     4081 ttgctcgcct tattgcgatg tgacgccggt ttccttgatg tcattgaggt atttcgcacg
     4141 ctcgccgctt acaaacaccc tgaactccgc gaccgacatt gggcgccacg cccgcgccgg
     4201 tcgcctcgga gacgcgctcc tgcggcgcag tgtgcggg
//
LOCUS       DASSRL010000607         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_208418, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000607 DASSRL010000000
VERSION     DASSRL010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208418"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFL49_12385"
     CDS             complement(<1..717)
                     /locus_tag="VFL49_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7912823.1"
                     /translation="MVAAGSLLAGTGSSQAQNQKPNIILILSDDFGYGDSGPYGGGEN
                     RGMPTPSLDRLAAEGMQFMSFYAQPSCTPGRAAVQTGRIPNRSGMTTVAFQGQGGGLP
                     AAEWTLGSVLKTGGYKTFFTGKWHLGEADYALPNAQGYDEMKYAGLYHLNAYTYGDPT
                     WFPDMDPALRAMFDRVTKGAMSGNAGQPAKEEFKINGQYVNTPLINGKEGVVGIPFFD
                     EYVEKASLDYLDRNARSTQPF"
     gene            1154..2206
                     /locus_tag="VFL49_12390"
     CDS             1154..2206
                     /locus_tag="VFL49_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET7912824.1"
                     /translation="MRKRNDRAKKGVERLPIANGGISRLAYQHALRAGIELDGQLQEA
                     GLTRAQIENPRAPIKVRDQIKFLNLVASALDDDFLGFRLAQACDLREIGLLYYVLASS
                     EILIDALQRAVRYSTIVNEGVSQTCIDSKYVGLSFDYVGVSRHPDRHQIEFWITALIR
                     TCRQLTGFRVVPERVRLVHHRTTKAELAEIFGDNIEFGATVDDVLFPISIRNSRVVSA
                     DPYLNNLLTSYCEEALAHRNRKRGSFQSAVENAIAPLLPHGKATVTDVARQLGLSQRT
                     FARRLSSEGLTFSELLENLRSDLASRYFIEGDMGVSEVAWLLGYREVGSFSHAFRRWT
                     GKTPREVLRAERTRQP"
     gene            2264..2788
                     /locus_tag="VFL49_12395"
     CDS             2264..2788
                     /locus_tag="VFL49_12395"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7912825.1"
                     /translation="MNSQDVRRRRAYQLCQADEYPHKNSFRPVTMQPLPRSEIEGCAA
                     MTDFTSDSMSDQQKTVAVVDDDPEMRCSLATLLDAVGYRAETYDSAETFLICASTCKA
                     SCLIIDINLGDITGVELAQQLSADGLKWPIIFMSGLADAAIRDQASAADPIAFLHKPF
                     RTQDLIEAIQKAIQ"
     gene            complement(2809..3489)
                     /locus_tag="VFL49_12400"
     CDS             complement(2809..3489)
                     /locus_tag="VFL49_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HET7912826.1"
                     /translation="MLAFERHSSGGIDRAELLPEMLIVAGWTGRDEKALHHHIEELAA
                     IGVPRPSSVPVFYRISTANLTQTGRLEVLGPDTSGEAEPVVVALQDGLWLGVGSDHTD
                     RKAETMGIALSKQLCAKVVGKSLWRLDDVARHWDQLVLRAYVTIGGERLKYQEGPLSA
                     MRNPSDLMSRAGGEAKFVAGTVMFCGTLGAIGGIRPAAQFTAVLVDPVLGREMSCEYS
                     INALPVVR"
     gene            3561..>3932
                     /locus_tag="VFL49_12405"
     CDS             3561..>3932
                     /locus_tag="VFL49_12405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7912827.1"
                     /translation="MLDTRRFRDVLGQFPTGVTIITTVTAGGDRLGMTISSFNSLSLD
                     PPLVLFSIWREASSFAAWQQAKRYAINVLSEDQEELSNKFARAKGEKWIGQTILGGKT
                     GVPLLPNTVIAFECEAYDRHDG"
BASE COUNT          808 a         1196 c         1130 g          798 t
ORIGIN      
        1 gaatggctgg gtcgaacgtg cattgcgatc gagatagtcg agggatgcct tttcgacgta
       61 ttcgtcaaag aacggaatgc cgaccacacc ttcctttccg ttgatcagcg gcgtgttgac
      121 gtactgtccg ttgatcttga attcctcttt cgccggctgc cccgcgtttc cggacatcgc
      181 gcccttggtg acgcggtcga acatggcgcg cagcgcaggg tccatatcgg ggaaccaggt
      241 cggatcgccg taggtgtaag cgttgagatg atagagaccc gcgtatttca tctcgtcata
      301 accctgcgca ttggggagcg cgtagtcggc ttcgccgaga tgccatttgc cggtgaagaa
      361 cgtcttgtag cctccggtct tgaggaccga gccgagcgtc cattccgccg ccggcaggcc
      421 gccgccctgg ccctggaagg ccaccgtcgt cattccgctg cggttgggaa tgcggcccgt
      481 ctgcaccgcc gcgcggcccg gcgtgcagct cggctgtgcg taaaacgaca tgaattgcat
      541 gccctcggcc gccagccggt cgagacttgg cgtcggcatg ccccggtttt cgcccccgcc
      601 atagggaccc gagtcgccgt atccgaagtc gtcggataga atgaggatga tattcggctt
      661 ttgattttgg gcctggctcg agcccgttcc ggccagcaaa gaacctgctg cgaccattgc
      721 cgcgcaggcg taaaggccga gtctgcatct aagcgaggat ttgatcaccg tagcctccat
      781 tggcgtcatc cggagtgggc acgcatcgct cgtccaagcc gctatcttcc gcgatgcacg
      841 cgtgccgcgc ctcgcaggat cagtgagtcc aatcttcctc cagcaagtga ggagggcgat
      901 attggacctt cgggaaatcg ccaccttcgc tcgctgttca ggcacgtttc agactctcgc
      961 ctttacgccc gagcaaaatg tcatttccat ttgcattgtt ggccgccgcg ccgcgcaatc
     1021 aattgcccaa aggggaaggt gtcgcaaacc tcgcttttgc aatgccatcg ggcagcgatg
     1081 tatcgccgta tttacgtcga tcatccagtc gactcgttgt aggctcagaa cgaggtggtc
     1141 aagcggggcg accatgcgaa agcgaaatga tcgcgccaaa aaaggcgtcg agcgccttcc
     1201 gattgccaac ggcggcataa gccggttggc gtatcagcac gcgctgcgcg ctggcattga
     1261 gcttgacggg caactgcaag aggccggcct gacgcgcgcg cagatcgaaa atccccgtgc
     1321 accaatcaaa gtgcgggacc agatcaagtt cctgaatctt gtcgcctccg ctttggacga
     1381 tgactttttg ggatttcgcc tggcccaggc ctgcgacctt cgtgagattg gcttgcttta
     1441 ctacgtcctc gcatcctcgg aaatcctgat cgatgccctg caacgcgcgg ttcgctacag
     1501 caccatcgta aatgaaggtg tgtcgcaaac atgcatcgac agcaaatacg tggggctctc
     1561 atttgactac gtgggcgtga gccggcaccc ggatcgccat cagatcgagt tctggatcac
     1621 cgccctgata cgtacctgtc gtcaactgac ggggtttcgc gtcgtccccg aacgtgttcg
     1681 gctggtccac caccgaacga caaaggcgga gctggctgaa attttcggcg acaacattga
     1741 gttcggcgca accgtcgacg acgttctatt tccaataagc atcagaaatt cccgagtcgt
     1801 cagcgccgat ccatatctca ataacctgct tacttcatat tgcgaggaag ccctcgccca
     1861 tcggaacaga aagcgaggct cgttccagtc agccgttgag aacgcgattg cgccattgct
     1921 gccgcatgga aaggcaacgg tgacggacgt agcgcggcag cttggactga gccagcgaac
     1981 atttgcacgg cgcctgtcgt cggaaggact caccttctcc gagttgctgg agaacctgcg
     2041 atctgatctc gcaagtcgat atttcataga aggcgatatg ggcgtctcgg aggttgcctg
     2101 gctgctgggc tatcgtgaag tcggctcctt ttcgcatgcc ttccgacgat ggacaggcaa
     2161 aacgccgcgc gaagtgctcc gcgcagagcg aacacgccag ccttgaacga acgcggctga
     2221 cctcaagcgc gtccgctgtg gcccccgcgg acaaacaatt ggcatgaatt cacaagacgt
     2281 ccgcaggcgt cgtgcgtatc aactttgcca ggcggacgaa tatccgcata aaaactcttt
     2341 cagacctgtg accatgcagc ctcttccccg gagtgagatc gagggttgcg cggccatgac
     2401 cgattttacg agcgactcaa tgtcagatca gcaaaagacc gttgccgtcg tcgatgacga
     2461 cccagaaatg cgctgttcgc ttgcgacctt gctggatgca gttggatatc gcgctgaaac
     2521 ctatgactca gccgagacgt ttctcatctg cgcctcaacc tgcaaagcca gttgcctcat
     2581 catagacatc aatctcggcg acattaccgg tgtcgaattg gcacaacaat tgtcggccga
     2641 tggcttgaaa tggccgatta tttttatgag cggcctcgcc gatgcggcca tcagagacca
     2701 ggcatcggcc gcggacccca tcgcattcct gcacaagccg tttcgaacac aggatttgat
     2761 cgaggccata caaaaagcga tccagtaatc ggccatgacc ggccggccct agcggacaac
     2821 cggcagggca ttaatcgagt attcgcagct catctcgcgc cccaggaccg gatccacgag
     2881 cacggccgta aactgcgcgg caggacgaat gcccccgata gcgccgagcg ttccacagaa
     2941 catgaccgtg cccgccacaa attttgcttc accgccggca cgtgacatca ggtcggaggg
     3001 attgcgcatc gccgacagtg ggccttcctg gtatttcagg cgttcaccgc caatggtgac
     3061 gtaggcgcga aggaccaatt gatcccagtg gcgcgcgacg tcatcgaggc gccaaaggct
     3121 cttgccgacg accttggcgc aaagctgttt cgacagcgcg atacccatcg tctccgcctt
     3181 gcggtcggtg tgatcggagc cgacgccaag ccacaagcca tcctgcagcg cgaccaccac
     3241 cggctccgcc tcgcccgagg tgtcaggccc aaggacctcc aggcgcccgg tttgcgtgag
     3301 atttgccgta ctgatgcggt agaacaccgg caccgatgac gggcggggca cgccgatcgc
     3361 cgccagttcc tcaatgtgat gatgcagcgc cttctcgtcg cggccggtcc accccgcaac
     3421 gatcagcatt tccggcaaaa gctcggcgcg atcgatcccg ccgcttgaat gacgttcgaa
     3481 ggcgagcatg gcttgttcct tgagttggcg atgagcactt cgtaacataa ccgcggacgc
     3541 aatttcaccg gcggaaatcc gtgttggaca ctcgacgttt ccgtgacgtg ctcggccagt
     3601 ttccgaccgg cgtcaccatt atcacaaccg tgacggctgg cggcgaccgg ctcggcatga
     3661 cgatcagttc gttcaattcg ctgtcgctcg acccgcctct cgttttgttc agtatctggc
     3721 gcgaggccag cagtttcgcc gcctggcagc aggccaaacg ctatgccatc aacgtgctga
     3781 gtgaggacca ggaggaattg tccaacaagt tcgcccgcgc taaaggggag aaatggatcg
     3841 ggcaaacgat attgggcggc aaaaccggcg tgccgctgct accgaacacc gtgatcgcgt
     3901 tcgaatgcga agcctacgac cgccacgacg gc
//
LOCUS       DASSRL010000608         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_260813, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000608 DASSRL010000000
VERSION     DASSRL010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260813"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1093
                     /locus_tag="VFL49_12410"
     CDS             215..1093
                     /locus_tag="VFL49_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7912828.1"
                     /translation="MTIRPRRSVLYMPGSNSRALDKAKTLPADGLILDLEDSVAPDAK
                     QAARQQVVEAVKAGGFGPREIFIRINGVETPGHADDLSAVAHAAPDVILVPKVSSAGT
                     LELIGRRLLDMGTDHKTRVWAMIETPLAIFNILSIAAAARDSETRLSGFVMGTNDLAK
                     DTRARLVPGRAPMLSWLSMCVAAARIHGIDILDGVYNDIGNADGFTRECVQGVDLGFD
                     GKTLIHPSQIEPCNAAFSPSSADVEQARKMITAFDLPENKGKGVVAIDGRMVERLHAD
                     MARRTVAIAEAILQSS"
     gene            1242..1469
                     /locus_tag="VFL49_12415"
     CDS             1242..1469
                     /locus_tag="VFL49_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7912829.1"
                     /translation="MIVCSCNVLSDHEVRTAANAAARRSTSHVYGCLGCSAQCGRCAR
                     TIRRIIDETLDGAKIGCPVGCACAMHAADPI"
     gene            1598..2089
                     /gene="bfr"
                     /locus_tag="VFL49_12420"
     CDS             1598..2089
                     /gene="bfr"
                     /locus_tag="VFL49_12420"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET7912830.1"
                     /translation="MKGDTRVVEFLNKALRHELTAVNQYWLHYRLLDNWGYKDLAKQW
                     RKESIEEMEHADKLVERIIFLDGFPNMQVLDPLHIGQNVKEVLDCDLAAEMSARALYQ
                     EAATHCHGVRDYVTRDLFEKLMSDEEHHIDFLETQLDLVAKLGLELYAQHHIGEMGED
                     SKD"
BASE COUNT          459 a          659 c          678 g          416 t
ORIGIN      
        1 atagcggtcc tgctcggcgc gcaagccgcc cgcaagaaac gcccgatagc cgttgaccaa
       61 ttgagccgga agcgacacct gattcaccca aatcgcactg tccggcccaa cacaatcacg
      121 tcgtcgcctg gtctgacaac ccgttgcaac gcggcagtgg cacgtccgcg ccgcgaaggc
      181 taggctttgt gtgttgcttg attggagacc gacgatgacg attcgcccgc ggcgcagcgt
      241 attgtacatg ccgggttcca acagcagggc gctggacaag gccaagacgc tgcccgccga
      301 cggcttgatc ctcgaccttg aggattcggt tgcgcctgac gccaagcagg ccgcgcgcca
      361 gcaggtcgtt gaggccgtaa aagcgggcgg cttcgggccg cgcgaaatct tcatcaggat
      421 caacggtgtt gagacgccag ggcatgccga cgatctttcc gccgtcgcgc acgccgcgcc
      481 cgacgtgatc ctggtgccga aagtatcgag cgccggcacg ctcgagctga tcggccgccg
      541 gctgctcgat atgggcaccg atcacaagac ccgagtctgg gcgatgatcg aaacgccgct
      601 tgcgattttc aacattcttt cgatcgcggc tgccgcgcgc gattccgaga cgcgcctgtc
      661 cggattcgtg atgggaacga acgatctcgc caaggatacg cgcgcgcgtc ttgtgccggg
      721 cagggcgccg atgctgtcct ggctttcgat gtgtgtcgct gcggcacgca tccacggcat
      781 cgatattctt gacggcgtgt ataacgacat cggtaacgcg gatggattta ccagagagtg
      841 cgtacaaggc gtcgaccttg gcttcgacgg caagacgttg atacacccga gccagatcga
      901 gccttgcaat gcggcgtttt caccgtcgtc ggctgatgtc gagcaggcgc gcaagatgat
      961 cacggcattc gacctgcccg aaaacaaggg taagggcgta gtcgcaatcg acggccgcat
     1021 ggtcgagcgg ctgcacgctg acatggcgcg gcggacggtc gcgattgcgg aggcaatttt
     1081 gcagtcgtcc tgaagacgtg atcgccaatt ttgatcaatc tggcggcggc ggaactggca
     1141 aaaagcaatt gaaccacaat agtttagaac gtttttaaaa agcactttta ttttgactca
     1201 gcgccggact gcggttagcg aatacccggc aaaggtgttt gatgatcgtc tgttcctgca
     1261 acgtattgag cgaccacgaa gtgcgaaccg cagccaacgc cgcggcacgt cgctcgacga
     1321 gccatgtcta tgggtgcctt ggctgcagcg cccaatgcgg ccggtgcgcg cgcacgatcc
     1381 gcaggatcat cgacgaaacc ctcgacggcg caaagatcgg ctgcccggtc ggctgcgcct
     1441 gcgcgatgca cgccgcggac ccgatctaag gtccggccga aacaatcccc atcgcgttca
     1501 aagccgctgc cggcactggc agcgcacggc acgtttattt gctagtgtcc ggacatgaag
     1561 gtggcgcttg ctgcgagcgc cacaacagga gcaggttatg aagggtgaca cgagagtcgt
     1621 cgagttcctc aacaaagcgc ttcggcacga gttgacggcg gtcaatcaat actggttgca
     1681 ctaccggctg ctcgacaact ggggctacaa ggacctggcg aagcaatggc gcaaggaatc
     1741 gatcgaggaa atggaacatg ccgacaagct ggtcgagcgc atcatttttc tcgacggttt
     1801 tcccaacatg caggtgctcg accctttgca catcgggcaa aacgtcaaag aagtgctgga
     1861 ctgcgatctg gccgccgaga tgtcggcccg cgccctttat caggaggccg cgacgcactg
     1921 ccacggcgtg cgcgattacg tgacacgcga cctgttcgag aaactgatgt ccgacgagga
     1981 acaccacatc gactttctgg aaactcagct cgacctcgtc gccaagctcg ggcttgaact
     2041 ctacgcgcag catcacattg gcgagatggg cgaggactcg aaagattgaa cccggcggca
     2101 tctctggggt tgtcgcggcg taacattgcg gtatcgatcg cggctgtctt gtccgcgcta
     2161 ccgctcgaca ttgctcgcgc cgagccgtcc gttagcccgg tgaaatcagc cg
//
LOCUS       DASSRL010000609         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1310621, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000609 DASSRL010000000
VERSION     DASSRL010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310621"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..774
                     /gene="lepB"
                     /locus_tag="VFL49_12425"
     CDS             19..774
                     /gene="lepB"
                     /locus_tag="VFL49_12425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7912831.1"
                     /translation="MSVTSGTKRKEGGIAETVRVIIHALIIALVIRTFFFQPFNIPSG
                     SMKDTLLVGDYLFVSKYTYGYSKYSFPLSPPIFSGRIPGDWLPQRGDVVVFRLPKDPS
                     TDYIKRVIGLPGDKIQVIDGVVSINGTPVKHERAPDFIETEEGVREAPVKRWKDTLPN
                     GVSFYTLDLVDNGFADNTQVYTVPPDHYFMMGDNRDNSTDSRFSQVGMVPLENIVGKA
                     QIIFFSVYEGERAWQFWRWPVSVRWSRLFTIVR"
     gene            798..1481
                     /gene="rnc"
                     /locus_tag="VFL49_12430"
                     /pseudo
     CDS             798..1481
                     /gene="rnc"
                     /locus_tag="VFL49_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
     gene            1474..2379
                     /gene="era"
                     /locus_tag="VFL49_12435"
     CDS             1474..2379
                     /gene="era"
                     /locus_tag="VFL49_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7912832.1"
                     /translation="MADVSTRCGFIALIGAPNAGKSTLLNALVGSKVSIVSRKVQTTR
                     ALIRGIAIDGEAQIIFVDTPGIFSPRRRLDRAMVTTAWTGAHDADLVGVLIDARNGIE
                     DEAEDILRRLEDVKTPRVLILNKIDVVSKENLLALAKHANAAAKFEATFMISALTGDG
                     VVDLKAWLALRVPHGPWLYPADQISEAPMRQLAAEITREKLFERLHQELPYHSTVETD
                     VWKELRKGDVRIEQTIYVERESQRKIVLGKGGQAIKAIGEAARKDIAEIAGVKVHLFL
                     FVKVRENWGDDPERYRAMGLEFPIE"
     gene            2409..3143
                     /gene="recO"
                     /locus_tag="VFL49_12440"
     CDS             2409..3143
                     /gene="recO"
                     /locus_tag="VFL49_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7912833.1"
                     /translation="MQWTDEGIVLGVKRHGEGNAILELMTREHGRHLGLVRGGSGSRL
                     KPLLQPGNSLSATWRARLDEHLGNYAVEALNLRAAGFFSSPHAIYGVGHLAALMRLLP
                     ERDPHLSLFESLDAIFGRLGDAAIAAPMIVRFELQLLSDLGFGLDLERCVATGVSGDL
                     VYVSPKSGRAVSRVAGEPWAEKMLRLPAFLRDRETIPTGPDLADGFALTGFFLARYAL
                     EPRGLALSDERAHFIAALNRALPSVA"
BASE COUNT          617 a          928 c         1052 g          661 t
ORIGIN      
        1 tggcgggacg aaaggcctat gagcgtgaca tctgggacaa agcgaaaaga gggcggcatc
       61 gccgaaacgg tgcgcgtaat catccacgcc ctgatcattg cgctggtgat ccgcacgttt
      121 ttctttcaac ccttcaatat cccgtcgggc tcgatgaaag acacgttgct ggttggcgac
      181 tacctgtttg tctcgaaata cacctatggt tacagcaagt attcgtttcc gctgtcgccg
      241 ccgatttttt ccgggcgcat tccgggcgac tggctgccgc agcgtgggga tgtggtcgtc
      301 tttcggctgc ccaaggatcc ctcgacggat tacatcaagc gcgtgatcgg attgccgggt
      361 gacaagatcc aggtcatcga cggtgtcgtc agcatcaacg gtacgccggt gaagcacgag
      421 cgcgcgccgg atttcataga gactgaagag ggggttcgcg aggcgccggt caagcggtgg
      481 aaagacacgc tgccgaacgg cgtgagcttc tacacgctcg atctggtgga taatgggttc
      541 gccgataata cgcaggtcta taccgttcca cccgaccatt atttcatgat gggcgacaac
      601 cgcgacaatt ccaccgacag ccggttcagc caggttggca tggtgccgct cgagaatatc
      661 gtgggcaagg cgcagatcat cttcttttcc gtctatgagg gcgagcgcgc ctggcagttc
      721 tggcgctggc cggtttcggt gcgctggagc aggctgttca cgattgtcag atgagccgca
      781 aggccaagga caggaccgcg ctcgaagaga ggatcggcta caaatttgcc gacaaggaac
      841 tgtttgaacg tgcgctgacg cacatttcgg cgctgtcggg cggcaatcgc gcggccagct
      901 atcagcggct tgagttcctc ggcgatcacg ttcttggtct cgtcatatcc gacatgctgt
      961 accgcgcctt tccgaaggcg aatgaaggcg aactgtcccg gcgtcttgca gatctggtgc
     1021 gcaaggaaac ctgcgccgag gttgcgcggg caatggacct cggtcccgtg ctcaagctcg
     1081 ggaattccga atcccacgcg ggcgggaggc ggcggaccac gatactggct gacgtctgcg
     1141 aggcgcttgt cggcgcggtg ttcatcgatg gcggctacaa tgcagccgat gagttgatcg
     1201 cgcgcttctg gaaggagcgg atgctcaagc cgctgcggcc gctgcgcgat ccaaaaacga
     1261 tcctgcagga gtgggcgcag gcgcgcggcc tcccgacgcc ggcctatcgc gagctggcgc
     1321 gcacgggccc ccatcacgat cccgaattcc gcgtggccgt cgtactgccg gaccgcccgc
     1381 cggcggaagg tctcggcagc tcaaagcgtg ccgccgaaca ggccgccgct gccgagatgc
     1441 tcacgcgcgt cggcgtgccg gcggacaagc ttgatggctg acgtttccac ccgctgcggc
     1501 tttatcgcgc tcatcggcgc acccaatgcc ggtaaatcga cgctgctgaa tgctctggtc
     1561 ggatcgaaag tcagcatcgt ttcacgcaag gttcagacca cgcgcgcatt gatccgcggc
     1621 atcgccatcg acggcgaagc tcagattatt ttcgtcgaca ctccgggaat attttcgccg
     1681 cgccgcaggc tcgatcgcgc gatggtcacg acggcttgga ccggggcgca cgatgccgac
     1741 ctggtgggcg tactgatcga tgcacgcaat ggcattgagg acgaagcgga ggacatcctc
     1801 cgccggctgg aagacgtcaa gacaccacga gttctcattc tcaacaaaat cgatgtggtc
     1861 tcgaaagaga atttacttgc gctggcgaaa catgccaatg cggcggcgaa gtttgaggca
     1921 accttcatga tttcggcgct gaccggcgac ggcgtggtgg atctgaaggc gtggctggcg
     1981 ctgcgtgtgc cgcacggccc ctggctttat ccggccgacc agatttccga agcgccgatg
     2041 cgtcagctcg ccgccgagat tacgcgtgaa aaactgttcg agcgtctgca tcaggagctt
     2101 ccctaccatt cgacggttga aaccgatgtc tggaaggagc ttcgcaaggg tgacgtcagg
     2161 attgagcaga caatctacgt tgagcgcgag agccagcgga aaatcgtgct cgggaagggt
     2221 ggccaggcca tcaaggcgat cggcgaggcc gcgcgcaagg acatcgcgga aatagcgggc
     2281 gtcaaagttc atttgttttt gttcgtcaaa gtgcgtgaga actggggaga tgatcccgag
     2341 cggtatcggg ccatgggcct ggagtttccg atagagtgac gtagaacgag ctggcgagaa
     2401 tctgtgccat gcaatggact gacgaaggca tcgtacttgg cgtcaagcgc cacggagagg
     2461 gcaacgccat cctcgagctg atgacgcgtg agcatggccg ccatcttggc cttgtgcgcg
     2521 gcgggtcagg ttcgcgcctc aagccgctcc tgcagccggg caatagtttg agcgccacct
     2581 ggcgggcgcg gcttgatgag caccttggca actacgccgt tgaggcgctg aatttgagag
     2641 cagccggctt cttttcatcc ccgcatgcga tctacggtgt cggtcacctg gccgcgctga
     2701 tgcgcttgct gcccgaacgc gatccgcatc tgagcctttt tgaatcgttg gacgccattt
     2761 ttggccggct cggtgatgct gcgatcgcgg cgccaatgat cgtgcgattt gaattgcagc
     2821 tgctgtccga ccttggtttt gggctcgatc ttgaacgatg cgtggcgacc ggtgtgtccg
     2881 gtgaccttgt ttacgtgtcg ccgaagtcgg gacgcgcggt gtctcgcgtg gcaggcgagc
     2941 cctgggcgga aaaaatgttg cggctgccgg catttctgcg cgatcgggaa acgataccga
     3001 ccggaccgga ccttgccgat ggatttgcgt tgactggatt tttccttgcc cgctacgcgt
     3061 tggagccgcg cggactggcg ctgagcgacg agcgcgcgca tttcattgct gcgctcaatc
     3121 gcgctttgcc aagcgttgcg taaagctggc cgcccgcgag gggcgaccag gactcttgcg
     3181 tgccgcagca ttaccagcgg tagcggtaac ggtaaccgta gccatatcga tacggacgat
     3241 agtaacgtgg tcccgtcc
//
LOCUS       DASSRL010000610         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1048583, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000610 DASSRL010000000
VERSION     DASSRL010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048583"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1225
                     /gene="rlmN"
                     /locus_tag="VFL49_12445"
     CDS             5..1225
                     /gene="rlmN"
                     /locus_tag="VFL49_12445"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7912834.1"
                     /translation="MTLTLQSADTIEKTPLERYVAPEKPSLVGMTRIELAAALGTVGV
                     PDAQRKMRVQQLWHWIYFRGLTSFEDMTSVSKELRAVLAEQFTLGRPEVVAEQVSVDG
                     TRKWLLRLPGEVQGERPHEVECVYIPDTERGTLCVSSQVGCTLNCTFCHTGTQRLVRN
                     LTAGEIVGQVMAARDRLGDWPGGTRPTDGVVPGGDRCITNIVMMGMGEPLYNFDAVRD
                     ALLVVADGEGIGISKRRITLSTSGVIPNIYRTGAEIGCMLAVSLHAVRDDLRNELVPL
                     NRKYPISELLQACRNYPGVSNAKRITFEYVMLKGVNDSTDDAKALIRLLRGIPAKINL
                     IPFNPWPGTKYECSDWEKIEKFSQVVFDAGYASPVRTPRGRDILAACGQLKSATEKLS
                     ARERMALRAMAMTD"
     gene            1376..1732
                     /locus_tag="VFL49_12450"
     CDS             1376..1732
                     /locus_tag="VFL49_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET7912835.1"
                     /translation="MKRNQAVGDVLNLILAIWLFLSPWIVGFAGLMPAAWTAWLSAIA
                     IAIFAVAALSAFSEWEEWINLILGIWVLISPWVMRVSTDKAPTVVLFLTGLAVAIIAA
                     IELWTLHRVPPRATVA"
     gene            1852..>2075
                     /locus_tag="VFL49_12455"
     CDS             1852..>2075
                     /locus_tag="VFL49_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912836.1"
                     /translation="MSLFGRIIVIIFGLIAAIIVAGITLAIGIVVPELAAMDSDPIER
                     VIFFFAAFFATALVVIYALLPAALVVAIAEV"
BASE COUNT          381 a          622 c          632 g          440 t
ORIGIN      
        1 cgacatgacc ctgacgttgc aaagcgccga caccatcgag aagacgccgc tcgagcgcta
       61 tgtggcgccc gaaaagccgt cactcgtcgg catgacgcgc atcgagctcg ccgctgcgct
      121 tggcaccgtc ggggtgcccg atgcgcagcg caagatgcgc gtgcaacagc tctggcactg
      181 gatctatttc cgcggactca cctcgttcga agatatgacg agcgtgtcaa aggagttgcg
      241 cgcagttttg gccgagcagt ttacgctcgg gcggccggaa gtcgtggccg aacaagtttc
      301 ggtcgatggc acccgcaagt ggctgcttcg cttgccgggc gaagtgcagg gcgagcggcc
      361 gcacgaggtc gaatgcgttt acatccccga cactgagcgc ggcacactct gcgtctccag
      421 ccaggtcggc tgcacgctca actgcacgtt ttgccacacc ggaacgcagc ggctcgtccg
      481 caatctcacg gcgggtgaaa tcgtcggcca ggtgatggcc gcccgtgatc gtctcggcga
      541 ttggccaggc ggcacccgtc caaccgatgg tgtcgtaccg ggcggcgacc gatgcatcac
      601 aaacatcgtg atgatgggta tgggcgagcc gctctacaat ttcgacgctg ttcgcgacgc
      661 cctgctcgtt gtcgccgacg gcgagggcat cggcatttcg aagcgccgca tcacgctatc
      721 cacgtccggc gtgataccca acatctaccg gaccggcgcc gaaatcggct gcatgctggc
      781 ggtatcgctg cacgcggtgc gtgacgactt gcgcaacgaa ctggtgcctc tcaaccgaaa
      841 atatcctatc tcggagctgc tgcaggcgtg ccgcaattat cccggcgttt ccaacgccaa
      901 gcgcattaca tttgaatacg tgatgctcaa gggcgtaaac gattccaccg acgacgcgaa
      961 agcactgata cggctgctca ggggcatccc agccaagatc aatctcatcc ccttcaatcc
     1021 gtggccgggc accaagtacg aatgctctga ctgggagaaa atcgagaaat tctcgcaggt
     1081 cgtgtttgat gctggatatg cctcgccggt gcgcaccccg cgtgggcgcg acattttggc
     1141 cgcatgcggc cagctcaaaa gcgccacaga gaagctctcg gcgcgggaac gcatggcgct
     1201 gcgcgccatg gcgatgaccg actagacgcg gtgaatcgaa cgaaaccgcc ggggcttgat
     1261 ctgcgttact tcggagagcc tccgcctgcc cggacgctgc cttgcgcgcg ggcatgacgg
     1321 gtccctttgg gggcgccaac cgggaaatcg atcccggctc tgaatggagg ttttcatgaa
     1381 aagaaatcaa gctgtgggcg acgtgctcaa cctcattctt gcgatctggt tgttcctgtc
     1441 gccttggatc gtgggctttg cagggttgat gcctgcggca tggaccgcgt ggctcagcgc
     1501 aatagcgatt gccattttcg ccgtcgccgc cctttcggct ttttccgaat gggaagaatg
     1561 gatcaatctc atccttggca tatgggtttt gatctctccc tgggttatgc gcgtatcaac
     1621 cgataaggcg ccgacggtcg tgctgttcct gaccggcctc gcggtcgcga tcatcgcggc
     1681 tatcgagctt tggacgttgc accgcgtgcc gccgcgggcg accgtcgcct aaggcgaacc
     1741 gtcgatggga aggccggcgt atgacgctcg gcctttccga ttgcgcaatt tgcgtggagt
     1801 tctggtattg aatggatgcc ggggggtctg tccggcagaa caggacacct gatgtccctc
     1861 ttcggccgga tcatcgtcat tatttttgga cttatcgccg ccatcattgt tgccggcatc
     1921 acactcgcga tcggaatagt tgtgccagag ttggcggcaa tggactccga tcccattgag
     1981 cgcgtgatct tctttttcgc ggcctttttt gcgacagccc ttgtagtcat ctatgcatta
     2041 cttccggccg cgcttgtggt ggcaatcgcg gaagt
//
LOCUS       DASSRL010000611         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1153237, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000611 DASSRL010000000
VERSION     DASSRL010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153237"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..959
                     /locus_tag="VFL49_12460"
     CDS             126..959
                     /locus_tag="VFL49_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7912837.1"
                     /translation="MFNRRTLIAAVTAAAVLSGAPVSAQDKSIVVASTTSTQDSGLFD
                     YLLPIFTKKTGIAVKVVAQGTGQALDTARRGDADVVFVHAKAQEEKFLTEGQGVKRFP
                     VMYNDFVLIGPKSDPAKIKGMKDVAKAFQTIKEKQASFISRGDRSGTHSAELKLWKDA
                     GIDIEKDKGPWYKAIGQGMGAALNTAGAGNAYVLSDRGTWIHFRNKGDLQILVEGDKR
                     MFNQYGVMLVSPQKHPNVKKEYGQQFIDWLVSPEGQKAIANYKINGEQLFYPNADDHS
                     A"
     gene            986..1747
                     /locus_tag="VFL49_12465"
     CDS             986..1747
                     /locus_tag="VFL49_12465"
                     /inference="COORDINATES: protein motif:HMM:NF002917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7912838.1"
                     /translation="MLAMTGPAAADTIQLFAAGSLKAALTGVAKAYEAASGNKIEARY
                     GPSGLLKNEISKGTKADVFASANMQHPQALHDEKKAGQVARFARNSLCALVKPGLKVD
                     SASLLDRMLDPGIKLGTSTPNADPSGDYAFEVFRKAESIKPGAQAILEKKALQLTGTA
                     SSAAAPAGRTVYGWHIAEARADIFITYCTNALAAQKENPGQQIVRPPPSLSVGADYGL
                     TIVNGAPPSAQQFADFILSPQGEKILASHGFAPGK"
     gene            1765..1971
                     /locus_tag="VFL49_12470"
     CDS             1765..1971
                     /locus_tag="VFL49_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET7912839.1"
                     /translation="MKLSARNQLKGKIVDVKKGTTTAHVRIDIGGQTITAAITNDSVD
                     ELRLEKGKTAYAIIKATSVMVGTD"
     gene            1992..3014
                     /locus_tag="VFL49_12475"
     CDS             1992..3014
                     /locus_tag="VFL49_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HET7912840.1"
                     /translation="MFLSRRTFVAAAASLAVLRPARAATVTDATGRALAVPEKVARVF
                     PAGPPAAILLYTLAPDLLLGWPRANRPEECMYMLPDVCTRPEVGRITGRGNTANLESV
                     IALKPDLILDVGSTSSTFVSLAERVQQQTGIPYALLDGRFDAVAETYRKLGALIGRKS
                     EAEKFARYADDTFKIIADRTASIAPSARPRVYYARGPRGLVTGLGGSINVETIEMLAQ
                     NVAGSSKGGLANVSIEQVLLWNPDVIVTIDQDFAATVRSEPAWASIKAVRDNRVHLSP
                     KMPFGWVDFPPSVNRLIGLRWLAKILYPDKFPEDMRAPTREFYTMFYHRTPDDAQIDH
                     VLAGRD"
     gene            3015..4028
                     /locus_tag="VFL49_12480"
     CDS             3015..4028
                     /locus_tag="VFL49_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7912841.1"
                     /translation="MTRSAMPGLAIATAVLIAGLLLAFTVGRYPVSLAELANVLLSKL
                     TAHRADVSPAVESVIMQVRGPRVLAAALVGAALAVAGTAFQGLFRNPLVSPDILGASS
                     GAALGAVIGIFFSLGVIAIQAFAFVGGLMAVAAVYAIGSAVRSRDPILVLVLTGVVVG
                     ALLGAGVGLVKYLADPYNQLPAMTFWLLGSLAAANVSDLLPLFGPVAIGSIVLIALRW
                     RMNVMSLPEEEARSLGVATGPLRIAIVAAATLTTSASVATAGIIGWVGLVVPHLARSL
                     VGPDFARLLPAAAILGGGYLLLIDTLARTAAEVEIPLGILTAVVGTPFFIWLLASMQR
                     NWS"
     gene            4025..>4313
                     /locus_tag="VFL49_12485"
     CDS             4025..>4313
                     /locus_tag="VFL49_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912842.1"
                     /translation="MNLSGHELTIGYPDHTVGRGLEVTLATGEVLALLGPNGGGKTTL
                     LKTLLGLLKAQAGEVRLNERPLDTYSIRDRARVVAYVPQVHIGTFAFTVETV"
BASE COUNT          821 a         1378 c         1328 g          786 t
ORIGIN      
        1 gcaccgcggg gagatgatcg aaaacacgcc ggcagcgcaa ttcttcactg caccgcgcac
       61 ggaggaagcc cgcaagttcg tcgcgggaga actactcgtc tgaataatga aagaaggagg
      121 atcacatgtt taatcgccgc accctcattg ccgccgttac agccgccgca gttctgtccg
      181 gcgcacccgt ttcggcgcaa gacaaatcaa ttgtggtggc gtcgaccaca tcgacgcagg
      241 attcaggcct gttcgactat ctgctgccga tcttcaccaa gaagaccggc attgctgtga
      301 aagtcgtcgc ccaggggacc ggccaggcgc tcgacactgc gcgacgcggc gacgcggatg
      361 tggtctttgt gcacgccaag gcgcaggaag agaaattcct caccgaaggc cagggcgtga
      421 agcgctttcc ggtcatgtac aacgactttg tgctgatcgg accgaagagc gatcctgcca
      481 agatcaaggg catgaaggat gtcgcaaagg cgtttcagac catcaaggag aagcaagcgt
      541 cctttatctc ccgcggcgac cggtcgggca cgcacagtgc cgagctcaag ctctggaagg
      601 acgccggcat agacatcgag aaggacaaag gcccctggta caaggcgatc ggccagggca
      661 tgggtgcagc cctcaacacc gcaggggccg gcaacgccta cgtcctctcc gaccgcggca
      721 cctggattca cttcaggaac aagggcgatc tgcagatcct ggtggaaggc gacaagcgca
      781 tgttcaacca gtatggcgtg atgctggtga gccctcagaa gcatccgaac gtcaaaaagg
      841 agtacggcca gcagttcatc gattggctgg tgtcgccgga aggccagaag gcgatcgcca
      901 actacaaaat caacggcgaa cagctgttct accctaacgc cgacgatcac agcgcctgat
      961 gcgcgcgggg gcaatgatac cggcaatgct tgccatgacg ggaccggcgg cggccgacac
     1021 catccagctg ttcgccgccg gcagcctcaa ggccgcgttg accggtgtgg ccaaagccta
     1081 tgaggcagcc agcggcaaca agatcgaagc gcggtacgga ccctccggcc tgctgaagaa
     1141 cgaaatttca aagggcacga aggccgacgt ttttgcctcg gcgaacatgc agcacccgca
     1201 ggcgctgcac gatgaaaaga aggccggcca ggtagcgcgg tttgcgcgca attccctgtg
     1261 cgcgttggtg aagccaggcc tgaaggtcga cagcgcgagc ctgctcgacc gcatgcttga
     1321 cccaggcatc aagcttggca cgtcgacacc gaatgccgat ccttccggcg attatgcttt
     1381 cgaggtcttc cgcaaagccg agtcgataaa gcccggcgcc caggccattc tggaaaagaa
     1441 ggctctgcaa ctgaccggca ccgcaagcag cgctgcggcg ccggccgggc ggacagttta
     1501 tggctggcac attgccgaag cgcgcgccga tatcttcatc acgtattgca ccaatgcgct
     1561 cgcggcgcag aaagaaaacc ccgggcagca gatcgtgcgg cctccgccga gcctttcggt
     1621 cggcgccgac tatggcctca ccattgtcaa cggagctccg ccgtcagcgc agcaatttgc
     1681 ggatttcata ttgtcgccgc agggtgagaa gattctggcg agtcacggct ttgcaccggg
     1741 aaagtgacag caaaaggggc gtccatgaaa ctcagcgcgc gcaatcagct caaaggcaag
     1801 atcgttgacg taaagaaggg cacgacgacg gcgcatgtcc gtatcgacat cggcggccag
     1861 accatcaccg cggccatcac caacgactcg gtcgacgagc tcaggctcga aaaaggaaag
     1921 accgcttacg ccatcatcaa ggcaaccagc gtgatggtgg gcacggatta gccgaggcat
     1981 acgcaccgtc gatgtttctg tcccgccgca cgtttgtcgc cgccgccgcg tcgctggcgg
     2041 tgttgcggcc tgcccgcgct gcgaccgtca ccgacgcgac gggtcgcgcg ctcgcggtgc
     2101 cggaaaaggt cgcacgcgtg tttccggcag gtccgccggc cgcgattttg ctctatacgc
     2161 tcgcgcctga tctgctgctc ggctggccgc gcgccaaccg tccggaagag tgcatgtata
     2221 tgctgccgga tgtctgcacg cgcccggagg tgggccgtat taccggccgc ggcaacaccg
     2281 ccaacctgga aagcgtgatc gcgctcaaac ccgatctcat cctcgatgtc ggctcgacta
     2341 gcagcacgtt cgtctctctg gccgagcgcg tgcagcagca gaccggcatt ccctacgccc
     2401 tgctcgacgg ccgcttcgat gcggtcgcgg aaacctaccg caagctcggc gcgctgatcg
     2461 gacggaaaag cgaggctgaa aaattcgcgc gctacgcgga cgatacgttc aagatcattg
     2521 ccgaccgcac cgcatcgatt gcgccgtctg cccggccacg cgtctattac gcgcgtggcc
     2581 cgcgtggtct ggttacaggg ctcggcggct cgatcaacgt cgagaccatc gaaatgctgg
     2641 cgcagaatgt cgccggcagc agcaaaggcg gtctcgccaa tgtctcgatc gagcaggttt
     2701 tgctgtggaa tcccgacgtt atcgtcacca tcgaccagga tttcgccgcg accgtccgca
     2761 gcgaacccgc ctgggcttcg atcaaggcgg tgcgcgacaa ccgcgtgcat ctgtcgccaa
     2821 aaatgccgtt cggctgggtg gattttcccc cctctgtgaa ccggcttatc gggcttcgct
     2881 ggctggcaaa gatcctctac cccgataaat ttccggagga catgcgggcg ccgacccgtg
     2941 aattctatac aatgttctat catcgcacgc cggacgacgc gcaaatcgat catgttctgg
     3001 ccgggagaga ctagttgacc cgatccgcga tgccgggact ggcgatcgca accgccgtgc
     3061 tcatcgccgg attgctgctc gccttcaccg tcggccgcta tccggtctcg ctcgccgagc
     3121 ttgcgaacgt tctgctttcg aaactcaccg ctcaccgcgc cgacgtatcg ccggccgtcg
     3181 aaagcgtgat catgcaggtg cgtggaccgc gcgtgttggc ggcagcattg gtcggcgcgg
     3241 cgctggctgt tgccggcacc gcattccagg ggctgtttcg caaccccctg gtctcgccgg
     3301 atatcctcgg cgcttcctcg ggcgccgccc ttggcgcggt gatcggcata ttcttctcgc
     3361 tcggcgtcat cgcaatccag gctttcgcgt tcgtgggcgg attgatggcc gttgccgccg
     3421 tctacgccat cggctcggcg gtccgctcgc gcgacccgat tctggtcctg gtgctgacgg
     3481 gtgtcgtagt cggcgcactg ctcggtgccg gcgtcgggct cgtgaagtat cttgccgatc
     3541 cctacaacca gctgccggcc atgacgttct ggctgctcgg cagcctggcg gcggcgaatg
     3601 tctccgacct gctgccatta ttcgggcctg ttgccattgg ctcaatcgtg ctgatcgcgc
     3661 tgcgctggcg catgaacgtc atgtcgctgc cggaagaaga ggcgcgctca ctcggtgttg
     3721 cgacaggtcc gctgcgcatc gcaatcgtcg ccgcggcgac gttgacgacc tccgccagcg
     3781 ttgccacggc cgggatcatc ggctgggtcg ggctggtggt gccgcatctt gcccgctcgc
     3841 tggtagggcc agatttcgcg cgtctgctcc ccgcggcggc tatcctcggc ggcgggtacc
     3901 tgctcctgat cgacacgctc gcgcgaaccg ccgcggaagt tgaaatccca cttggcatat
     3961 tgaccgccgt ggtcggcacg ccctttttca tctggctgct ggccagcatg caaagaaact
     4021 ggtcgtgaat ctctccggcc atgagctcac catcggttac cccgaccaca cggtcggccg
     4081 cgggctcgag gtgacactcg caaccggcga ggtgctcgcg ctgcttggcc caaacggcgg
     4141 cgggaagaca acgttgctca agaccttgct tggtctgctc aaggcgcaag cgggcgaggt
     4201 gcgactcaac gaaagaccgc tcgacaccta ttcgatccgc gatcgggcgc gcgtcgtcgc
     4261 ctatgttccc caggtgcaca tcggcacctt tgcgtttacc gttgaaaccg tcg
//
LOCUS       DASSRL010000612         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1625994, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000612 DASSRL010000000
VERSION     DASSRL010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625994"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFL49_12490"
     CDS             <1..778
                     /locus_tag="VFL49_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7912843.1"
                     /translation="VEVALARAYNRWLSERVLAHEPRIRSMLYLPFNDADAAYRTVQE
                     FGQAKGVVGFMVTSARYRPVHHNSYMKTYALLEEMNLPLAFHAGYNWNDQTVGMMNRF
                     ISVHALGFVFYNMVHLANWITNGLPERFPKLKVLWVESGLAWVPFMMQRFDNEYMMRS
                     SEAPALKRKPSEYMRDMYFSSQPMEMTDMAALEQTFRMINAETQLLYSSDYPHWDFDL
                     PSTIYDLPFLTEQAKRNILGGNAMKVFNLKLPAQKSARVA"
     gene            788..1774
                     /locus_tag="VFL49_12495"
     CDS             788..1774
                     /locus_tag="VFL49_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912844.1"
                     /translation="MREDIVRTFIAGVSAALLFASGAFAQDPAADYPNRPIKIIVCVP
                     AGGGVDTVTRIVAEGLHQKLGQPVVVENRAGAAGNIGAEAVFTSDPDGYTLLAAQPAP
                     LTVNPLLYKKMNFDPTQFEPVAIMTSIANVLLVRPDFPAKTAKEFIDYVKASPGKVNY
                     ASQGIGTTSHLTAALFERLVGTKLVHVPYKGTAPALNDIIASHVDFIFMELASAIKLH
                     QAGKARILAVATNKRIPNLPDIPTFDEVGVKGFESGTWNAIAAPPKTPTAIVAKLNKA
                     VNEVLKSPEVQDHFIKINLHAAEGSPAEAKAFIRKETEVWGAVIKDAHVEPH"
     gene            1894..>2678
                     /locus_tag="VFL49_12500"
     CDS             1894..>2678
                     /locus_tag="VFL49_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET7912845.1"
                     /translation="MDERTREPMKITAEDINPRYNWGRHLPVLGTMGVDFEERVDYRR
                     MHRYRLGRAQAALEASDLGALLLFDDNNIRYVTSTKIGEWARDKLSRWVLLTRGGDPI
                     LWDFGSAAVHHKLYSPWLKPENCKAGLVGLRGTVDPAFGLMKRHAEEMASILKSAGVA
                     KMPIGVDIIEPPMAFELEKAGVKIKDGQQIMLSAREVKSADEIALLNRAAAMVDGAYD
                     VVNEMLRPGVRENDIVAEVNKFLYTHGSDDVEAVNAISGERCNP"
BASE COUNT          583 a          811 c          772 g          512 t
ORIGIN      
        1 ggtcgaggtc gcgctggcgc gcgcctacaa tcgctggctc agcgaacgcg tcctggcaca
       61 tgagccgcgc atccgctcga tgctctatct cccgttcaac gacgcggacg ccgcttaccg
      121 caccgtccag gaattcgggc aggccaaagg cgtcgtcggt tttatggtga cctcggcgcg
      181 ctatcgtccg gttcaccaca attcctacat gaagacctat gcccttctcg aagagatgaa
      241 tttgccgctc gccttccacg ccggctacaa ctggaacgac cagaccgtcg gcatgatgaa
      301 ccgtttcatc tcggtccacg cgctcggttt cgtgttctac aacatggtgc atctggccaa
      361 ctggattacc aacggcctgc cggagcggtt tccaaaactg aaggtgctgt gggtcgagag
      421 cggccttgcc tgggtgccgt tcatgatgca gcgcttcgac aacgagtaca tgatgcgctc
      481 gtcagaagcc cctgccctga agaggaagcc aagcgaatac atgcgcgaca tgtatttttc
      541 gtcgcaaccc atggaaatga ccgacatggc tgcacttgag cagacgttcc gcatgatcaa
      601 cgctgaaacg caattgcttt attcgtcgga ctatccgcat tgggacttcg atttaccctc
      661 gacaatctac gatttgccgt tcctgacgga acaggcaaaa cggaatattc ttggcggcaa
      721 tgccatgaag gtgttcaatc tcaagctgcc ggcgcaaaaa tcggctcgtg tcgcataaat
      781 agccggcgtg agggaggata tcgtgcgcac atttatagca ggggtatcgg cggcactgct
      841 gttcgcctcg ggcgcgttcg cgcaggatcc ggccgcggat tatccgaaca gaccgatcaa
      901 gattatcgtc tgtgtcccgg cgggcggcgg cgtcgatacg gtgacccgca ttgtcgccga
      961 gggactgcat cagaagctgg gccagccggt cgtcgtggaa aatcgcgcag gtgctgcagg
     1021 caatatcggc gcggaggcag tgttcacgtc ggatcccgac gggtacacgc tgctcgccgc
     1081 gcagccggca ccgctgacgg tcaaccccct tctttacaag aaaatgaatt tcgacccgac
     1141 gcagttcgag ccggtggcga tcatgacctc gatcgccaac gttttgctgg tgcgcccgga
     1201 ttttccggcg aagacagcca aggaattcat cgattatgtg aaagcaagcc cgggcaaggt
     1261 caattacgcg tcgcaaggaa ttgggacgac gtcacatctg accgcagcct tgttcgagcg
     1321 gctcgtcggc accaagcttg ttcacgttcc ctacaagggg accgcgcccg cgctcaacga
     1381 catcatcgca agccatgtcg attttatttt catggagctg gcttcggcca tcaaactgca
     1441 ccaggccggc aaggcaagaa ttctggcggt ggcgacaaac aagcgaattc ccaacctgcc
     1501 ggacattcct acattcgacg aagtgggcgt gaaaggattc gagtccggca catggaacgc
     1561 tatcgccgct cctccgaaaa caccgaccgc gattgtcgcc aagctcaaca aggctgtgaa
     1621 cgaagttctc aaaagccccg aagtgcagga tcactttatc aagattaacc tgcacgcagc
     1681 cgagggctcg cccgccgaag ccaaggcgtt tattcggaag gaaaccgaag tgtggggcgc
     1741 tgtgatcaag gacgcccacg tggagcccca ttgactgcat tccgccaccg gtccgatggt
     1801 gcgacagagc cgccgactgg gcctataatg caaatgaccc accggccata acgccggcaa
     1861 tcgatatcgc gaataccgcg taaggagacg accatggacg agagaacgcg cgaaccgatg
     1921 aagatcaccg ccgaagacat caacccgcgc tacaactggg gccggcactt gccggtgctc
     1981 ggcaccatgg gcgtcgattt tgaagaacgt gtcgattacc gccgcatgca ccgctatcgc
     2041 ctcggccgcg cgcaggccgc gctggaggcc tccgacctcg gtgcgctgtt gctgtttgac
     2101 gacaacaata tccgctacgt tacctcgacc aagatcggtg aatgggcgcg cgacaagctc
     2161 tcgcgctggg ttctcctcac gcgtggcggc gatcctatcc tgtgggattt cggctcggcc
     2221 gcggtccacc acaagctcta ttccccctgg ctcaagccgg agaactgcaa ggccggcctg
     2281 gtcggcttgc gcggcaccgt tgatccggct ttcggcctga tgaagcgtca cgccgaggaa
     2341 atggcctcaa ttctcaagtc ggcgggcgtc gccaaaatgc cgatcggcgt cgatatcatc
     2401 gaaccgccaa tggcgtttga gctcgagaaa gccggggtca agatcaaaga cggccagcag
     2461 atcatgctga gcgcgcgtga agtcaaatcc gccgacgaaa tcgcgctgct caaccgcgcg
     2521 gccgcgatgg tcgacggcgc ctatgacgtc gtcaatgaaa tgctgcggcc cggtgtgcgc
     2581 gaaaacgaca ttgtcgccga ggtcaacaaa ttcctttaca cgcacggctc cgacgacgtg
     2641 gaagcggtca acgccatttc cggcgagcgc tgcaatcc
//
LOCUS       DASSRL010000613         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_86181, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000613 DASSRL010000000
VERSION     DASSRL010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86181"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFL49_12505"
     CDS             complement(<1..378)
                     /locus_tag="VFL49_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7912846.1"
                     /translation="MRPLALNPLFASLRSLTGVGPRLERLYAQLLDRAAPRVVDLLFH
                     MPSGAIDRRARPKLRDVQPGQVVTVSVTVDKHRPSPPHRSRAPYRIYAHDDTGDIILT
                     YFNARRDYLEKLLPVGEMRYVSGT"
     gene            511..801
                     /locus_tag="VFL49_12510"
     CDS             511..801
                     /locus_tag="VFL49_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HET7912847.1"
                     /translation="MTTGTNLSSEGLDPRRRRLLFRCWHRGIREMDFVLGRFADAHLA
                     ALTESELGVLERWLEVPDQQIFAWVNGMETTPPEFDTALFHRLRDSHRKETA"
     gene            798..>3375
                     /locus_tag="VFL49_12515"
     CDS             798..>3375
                     /locus_tag="VFL49_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7912848.1"
                     /translation="MSTSVKSPAALLKPGKPLVLAQVADGAEGLVLADLARAIAARKD
                     APAVSLAVICRDGQRMAALSRALTFFGPDLEIVEFPAWDCLPYDRVSPNAAVVAQRMT
                     ALSRLVRVKGRDKPSILLTTINAALQRVPARDFVSTHALSVAPGNVIGMQGIVDWLEL
                     NGFARASTVREPGEYAVRGGILDLYPPGMDMPVRLDFFGDALETIKAFDAQTQRSEEQ
                     LRRLDLVPVAEFQLVTETIRRFRTGYIAEFGAATPDDLLYESVSEGRRYPGMEHWLPL
                     FHDRLETLFDFLPGTAVALEHLAEDAAHERFSQITDYHDARRDALKQGVTPVYKPLPS
                     ARLYLGEQEWKSRLDAAALARLTPFAVPEANAIEVGARAGHNFTAERAEPGANVFEAV
                     SAHVLALQAAGKRVAIALWSEGARERMSHVLADHRLHNLTPVADWSAAIALPKTSIAL
                     AVLGIESGFETDDAAIISEQDILGDRLVRPRRAARRAENFIAEATSLAPGDLVVHVDH
                     GIGRFAGLQAIEAAGAPHDCLEIHYAEGAKLYLPVENVDLLSRYGSEEATVDLDRLGG
                     GNWQARKARMKSRIREIAGELIKIAAERQLREAPRLTVGPGAYDEFCAGFPYEETDDQ
                     LAAIDATIKDLGSGRPMDRLICGDVGFGKTEVALRAAFLAAIGGKQVAVVVPTTLLAR
                     QHFKTFSERFRGFPVNVAQASRLVPAAELAKVRAGLADGTIDIVIGTHALLGKNVKFK
                     DLALLVVDEEQHFGVAHKEKLKTLRSEVHVLTLTATPIPRTLQLALTGVRDLSIIASP
                     PIDRLAVRTFVSPFDPITVREALLREKYRGGQAFYVCPRIEDLAGGKDFLDKTVPEVR
                     VAV"
BASE COUNT          579 a         1060 c         1124 g          612 t
ORIGIN      
        1 ggtaccggac acgtatcgca tttctccgac aggcaacagc ttttcgagat agtcccgccg
       61 cgcattgaaa taggtgagga tgatgtcgcc ggtatcgtcg tgtgcgtaga tgcggtaggg
      121 cgcgcgcgaa cggtgcggcg gcgagggccg gtgtttgtcg accgtcaccg agactgtgac
      181 gacctggccc ggctgcacgt cgcgcaattt gggacgcgcg cgcctgtcga tcgcgcccga
      241 cggcatatga aacagcaaat cgacgacgcg cggcgcggcg cggtcgagca actgtgcata
      301 aagcctctca agccttgggc cgacgcccgt caggctcctg agcgaagcaa acagaggatt
      361 aagcgccaaa ggccgcatgg agacatgctg gcttgggggg aatttcggcg caatgcagcc
      421 ctgtgcaaag gaacggctga cattgcagga cgaggcctct atataaaccc cgcccggccc
      481 cgtccgggtt tttggcgttg gagatatgcg gtgaccacag gaacgaacct gtcgagcgag
      541 gggctggatc cgcgccgccg ccgtcttctc ttccggtgct ggcatcgcgg tatccgcgaa
      601 atggactttg tgcttggccg atttgccgat gcgcatctcg cagcgttgac ggaaagcgag
      661 ctcggcgtgc tcgagcgctg gctcgaggtc ccggatcagc agattttcgc ctgggtcaat
      721 ggaatggaaa cgacaccgcc ggaattcgac actgctctct ttcaccgtct ccgcgattcc
      781 caccgcaagg agacggcttg agcacgagcg tcaaatcacc tgccgccttg ctgaagccgg
      841 gcaagccgct tgtcctggcg caggttgcgg acggcgccga gggcctggtg cttgccgatc
      901 tcgcgcgcgc gatcgctgct cgcaaggacg cccctgccgt gtcgctcgcc gtcatatgcc
      961 gcgacggaca gcgtatggct gcgctgtcgc gcgcgctgac cttttttggt cccgatctcg
     1021 agatcgtgga atttcctgcc tgggactgcc tgccctacga ccgcgtttcg ccaaatgcgg
     1081 ctgtcgtcgc gcagcgcatg accgcattgt cacgcctcgt gcgcgtgaaa gggcgtgaca
     1141 agccgtcgat cctgctgacg acgatcaatg ccgcgctcca gcgcgtgccg gcgcgggact
     1201 ttgtgtccac gcatgcgctg tcggttgcgc ccggtaacgt cattggcatg cagggaatcg
     1261 tcgactggct cgagctcaac ggatttgcgc gcgcttcgac tgtgcgagag ccaggcgaat
     1321 atgccgtgcg tggcggcatc cttgaccttt atccgcctgg catggacatg ccggttcgcc
     1381 tcgatttttt cggcgacgcg ctggaaacga tcaaggcttt cgatgcgcag acccaacgca
     1441 gcgaagagca gttgcggcgg ctcgatcttg tgcccgtcgc ggagtttcag ctggtcaccg
     1501 agaccatccg ccgctttcgc accggctaca ttgccgagtt cggcgcagct acgccggatg
     1561 acctgttgta tgaatcagtg agcgaaggcc gccgctatcc cggcatggag cattggctgc
     1621 ctttgtttca tgaccggctc gaaactttat tcgattttct tccggggacg gccgtcgcgc
     1681 ttgagcatct cgccgaggac gcggcgcatg agcgcttttc acagataacc gactatcacg
     1741 atgcgcgtcg cgatgccctc aagcagggcg tgacgccggt ctacaagccg ctgccgtcgg
     1801 cccggctcta tctcggcgag caggagtgga aatcgcgcct cgatgcggcg gcgctggcgc
     1861 ggctgacgcc tttcgcggtg ccggaggcga acgcgatcga ggtgggcgcg cgcgcgggtc
     1921 ataatttcac ggccgagcgc gccgagccgg gcgccaacgt cttcgaggcg gtaagcgccc
     1981 acgtgcttgc attgcaggca gcgggcaagc gcgtggcgat agcattgtgg agcgagggcg
     2041 cccgcgaacg catgagccac gtactcgctg accaccggct tcacaatctt acgccggtcg
     2101 ccgactggag cgcggcaatc gctttgccga agacgtcgat tgcgctcgct gtgctcggca
     2161 tcgaatccgg tttcgagacc gacgacgcgg cgatcatcag cgagcaggac attctcggcg
     2221 accggctggt gcggcccagg cgcgcggcca ggcgcgcgga aaacttcatc gccgaggcaa
     2281 ccagcctcgc ccccggcgat ctggtggtgc atgtcgacca cggcatcggc cggtttgcgg
     2341 ggctgcaggc gatcgaggca gcaggtgcgc cgcatgactg cctggaaatt cactacgcgg
     2401 agggcgccaa gctctatctg ccggtcgaga acgtcgacct gctgtcgcgc tacggctcgg
     2461 aggaggcaac agtcgacctc gaccgcctcg gtggcggcaa ctggcaggcg cgcaaggcgc
     2521 gcatgaagag ccgcatccgc gagatcgcgg gcgagctgat caagatcgcc gccgagcgcc
     2581 agttgcgcga ggcgccgcgc ctgacggtcg gtccgggcgc ctatgatgag ttctgcgccg
     2641 gctttcccta tgaggaaacc gacgaccagc tggcggcgat cgatgcaacg atcaaggacc
     2701 tgggctcggg acgtccaatg gaccgcctca tctgcggcga tgtcggcttc ggcaagaccg
     2761 aagtcgcgct gcgcgcggct ttccttgcag cgatcggcgg caagcaggtc gccgtggtcg
     2821 tgccgacaac gctgctggcc cgccagcact tcaagacgtt ttccgaacgg ttccgcgggt
     2881 ttcctgtgaa cgtcgcgcag gcatcacggc tggtaccggc cgccgagctg gcaaaggtgc
     2941 gggcgggcct ggccgacggc acgatcgaca tcgtgatcgg cacgcacgct ttgctcggca
     3001 agaacgtgaa gttcaaggat ctggcgctgc tggtggtcga cgaggagcaa catttcggcg
     3061 tcgcgcataa ggagaagctc aagacgctgc gctcggaagt gcacgtgctg acgctgacgg
     3121 cgacgccgat cccgcgcacg ctgcaactgg cgctgaccgg ggtacgcgat ctctcgatca
     3181 tcgcatcgcc gccgatcgac cgtctggcag tgcgcacgtt tgtctcgccg ttcgacccga
     3241 tcacggtccg cgaggcgctg ctgcgcgaga aataccgcgg cggccaggcc ttctatgtct
     3301 gcccccgcat cgaggatctg gccggcggca aggatttcct cgacaaaacc gtgccggaag
     3361 tgcgcgtcgc cgtcg
//
LOCUS       DASSRL010000614         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_541119, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000614 DASSRL010000000
VERSION     DASSRL010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541119"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFL49_12520"
     CDS             complement(<1..600)
                     /locus_tag="VFL49_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912849.1"
                     /translation="MITAADVAPPVSPPALHAASLRERLLLIVLFITVLASSVAFIEP
                     SPHDGLMGVLAVVGLIAGVRMERALVLPFLLLLIWNFAGMLALMHVPGEEKTIQYTAT
                     SVYLAIAGMLFACLFAQNTMARLSAMRIAYVTTAVMATFLGIAGYFDLFGTGQAFAPL
                     GRALGAFKDPNVFGPFLIWPALFLLYRLIARGVRFADMLL"
     gene            complement(597..2150)
                     /locus_tag="VFL49_12525"
     CDS             complement(597..2150)
                     /locus_tag="VFL49_12525"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET7912850.1"
                     /translation="MGNSDIRPSLSAAGAAAVADRMIARARKASKARALTPEALEVAA
                     RPAPRPISPIVLAGFVRMAEFTLVIMVGLALYAIYLPPMHDLVWRYTAAIFVIALLST
                     LAFQTADIYQVQAFRGYEKQYMRLASAWSFVFLIVIGASFFAKAGDMFSRVWLGSYYI
                     AGLCALMTSRRLVFLLVRKWTREGRLNRRTVIVGGGDAGAGVIEELRRQRDTGVDIIG
                     LFDDRGDDRSSAECAGERKLGTVDDLVEFGRRTRVDLVIFSLPISAETRILQMLKRLW
                     VLPVDIRLAAHANKLRFRPRSYSYIGNVPVLDVFDRPIADWDVVMKWLFDKLIGGIML
                     LAALPVMAVIALAIKLDSKGPVLFKQRRYGFNNDLIGIYKFRSMYIDAADPAADRLVT
                     KNDPRVTRVGRFIRKTSLDELPQLLNVVLKGNLSLVGPRPHAINAKAEAKLYDEAVDG
                     YFARHRVKPGITGWAQINGWRGETDTHEKIQQRVEHDLYYIENWSVLFDFAIMLRTPL
                     ALLKTENAY"
     gene            complement(2265..>2464)
                     /locus_tag="VFL49_12530"
     CDS             complement(2265..>2464)
                     /locus_tag="VFL49_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET7912851.1"
                     /translation="LVAPQDVGALAEVIQDNLDHPEGTADTARRLRERVSQLFSVKSM
                     VDGVLAAYQAGLDRLRQNARR"
BASE COUNT          508 a          762 c          786 g          408 t
ORIGIN      
        1 gaggagcatg tcggcgaagc ggacgccgcg tgcgatcagg cgatagagca gaaacagcgc
       61 cggccagatc aggaacgggc cgaacacatt ggggtccttg aatgcgccga gcgcgcgacc
      121 aagcggcgca aaagcctgtc cggtgccgaa cagatcgaaa tagccggcga tgcccaggaa
      181 agttgccatc accgcggtcg tcacataggc gatccgcatc gcgctcagcc gcgccatggt
      241 gttctgcgcg aacaggcagg cgaacagcat cccggcgatc gcgaggtaga ccgaggtcgc
      301 cgtgtactga atggtctttt cctcgccggg gacgtgcatc aacgccagca tgccggcgaa
      361 attccagatg agcagcagca gaaacggcag gaccagcgcg cgttccatac gaacgccggc
      421 aatcagcccg accactgcaa gcacacccat gaggccatca tgcggggagg gctcgatgaa
      481 ggcgacagag cttgccagga cggtgatgaa caggacgatc agcagcagcc gttcccggag
      541 cgatgccgcg tgcagcgccg gcggtgaaac tggcggtgcg acatcggcgg ccgtgatcaa
      601 taggcgttct cggtcttgag cagcgcgagc ggtgtgcgca gcatgatcgc gaaatcgaaa
      661 aggaccgacc agttctcgat gtaatagagg tcgtgttcga cacgctgctg gatcttttcg
      721 tgcgtgtcgg tttcgccgcg ccatccgttg atctgcgccc aacccgtaat gccgggcttc
      781 acgcggtggc gagcgaagta gccgtcgacg gcttcgtcat agagcttggc ttctgccttg
      841 gcgttgatgg cgtgcggccg cgggccgacc agagacagat tgcctttcag cacgacgttc
      901 aggagctgcg gcagctcgtc gaggctcgtc ttgcgtatga aacggccgac gcgcgtcact
      961 cgcgggtcgt ttttggtcac gagcctgtcg gcggccgggt cggcggcgtc gatatacatc
     1021 gagcggaatt tatagatccc gatcaggtcg ttattgaagc cgtagcgcct ctgcttgaac
     1081 agcaccgggc ccttgctgtc gagcttgatg gcaagcgcaa tcaccgccat gaccggcaag
     1141 gccgcgagca gcatgatgcc gccgatcagc ttgtcgaaaa gccacttcat cacgacgtcc
     1201 cagtccgcga tcggccggtc gaagacgtcg agcaccggca cgttgccgat ataggagtag
     1261 gagcgaggcc ggaagcgcag cttgttcgca tgcgcggcga gacggatgtc gaccggcaac
     1321 acccaaagcc gcttcagcat ctgaaggatg cgcgtttccg ccgagatcgg cagcgagaag
     1381 atcaccagat cgacccgcgt gcggcgcccg aattcgacga gatcgtcgac cgttccgagc
     1441 ttccgttctc cggcacattc ggcgctgctt cgatcgtcgc cgcgatcgtc gaacagcccg
     1501 ataatgtcca cacccgtgtc tctttgccgg cgcaattctt cgatcacacc cgcaccggca
     1561 tcgccgccgc cgacgataac cgtgcgccgg ttcagccggc cttcgcgcgt ccatttgcgg
     1621 acaagcagaa acaccagccg gcgtgatgtc atgagagcgc agagaccggc gatgtagtat
     1681 gagccgagcc aaacgcgcga gaacatgtcg cccgccttgg cgaagaacga cgccccgatc
     1741 acgatcagga atacgaacga ccaggccgaa gccagccgca tgtattgctt ctcgtatccg
     1801 cggaatgcct gcacctggta gatgtcggcg gtctggaagg cgagcgtgga gagaagtgcg
     1861 atgacgaata ttgcggccgt atagcgccag accagatcgt gcatcggcgg aagatagatc
     1921 gcgtagagcg caagcccgac catgatcacg agggtaaatt cagccatgcg cacgaaaccg
     1981 gcgagcacga tcggtgagat cggccgcggc gcgggcctcg cggcaacctc cagcgcctcc
     2041 ggtgtcagcg cgcgggcctt agaggctttg cgcgcgcgag cgatcatccg gtcggcgacc
     2101 gcggctgcgc ccgctgcgct cagtgaggga cggatgtcag agttacccat gcgcgcgcca
     2161 aaaaagcacc cgcggtgacc aggtgcacgg cgggcgcccc caattccttg aattttctag
     2221 agaagaagtc tcaaaaaaga cttagcgcgg cgtcgacaag tcttctaacg gcgggcgttt
     2281 tgccgcagcc ggtcgagacc cgcctggtag gctgcaagga cgccgtcaac catgctcttc
     2341 accgaaaaca gctgcgacac ccgctcccgc agccgccgtg cggtgtccgc ggttccctcc
     2401 ggatggtcga gattgtcctg gataacctcg gccaacgcgc cgacatcctg cggtgcgacc
     2461 agcc
//
LOCUS       DASSRL010000615         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1608251, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000615 DASSRL010000000
VERSION     DASSRL010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608251"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..630)
                     /locus_tag="VFL49_12535"
     CDS             complement(223..630)
                     /locus_tag="VFL49_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7912852.1"
                     /translation="MKFEGGCYCRKIRYVAEGEPRLKAQCHCRECQYISGGAPNMFML
                     MAPEGFRYTSGEPRKFTRSDLESPVTREFCGECGTHLTTRRPGLPLVILKVGTLDNPD
                     LFGTPQMAIYTVDRQPFHVIPDGLQTFERLPPR"
     gene            complement(670..1725)
                     /locus_tag="VFL49_12540"
     CDS             complement(670..1725)
                     /locus_tag="VFL49_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HET7912853.1"
                     /translation="MSAENLQALRPSVAMAAALAPGILLSALVAAIGYIAAPFVAKVV
                     PIPNMVIALVVGIALNPLAARPAVQPGMNFCVKTLLRWAVALLGLRVGLGDIAALGLQ
                     TAVLIVMAMAATVASGFVFARWYGRSAGFGALVGVGTAVCGASATLATSTVVPEYKGK
                     AADIAFVVVAVNALATLAMLVYPPLCIALGFDAQTTGVMLGGTIHDVAQVVGAGYAVS
                     EPVGNTAVIVKLFRVFLLLPVVLAVGWHFTRMGMRHGEARVPVPVFAIVFLLLCVLNS
                     AMPLVPSWLPVYAPAKSLLVEISTWGLLLAIGALGLGTSVKTIIGLGWRHITTVLGTT
                     AVIFVVVTGGLMLMRFI"
     gene            complement(1735..>2082)
                     /locus_tag="VFL49_12545"
     CDS             complement(1735..>2082)
                     /locus_tag="VFL49_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA synthetase subunit alpha"
                     /protein_id="HET7912854.1"
                     /translation="LGTTTRDALEALDKDGRTDAIVIVGEIGGAMEEDAAACAKTMAK
                     PMVAFIAGAAAPTGKKMGHAGAIVTGNAGSYASKRQALEAAGVTVVDTPSQIAAAVAA
                     GLQSGRSKVAASQ"
BASE COUNT          408 a          684 c          645 g          345 t
ORIGIN      
        1 cggaggatct ccacctccga ctttcaacgc tgctttttcg cttgagggag atgcgacacc
       61 gattcccggg gctctcccgc ttttcgcctc aggcctgggc gcactcggtc tgctcggctg
      121 gcgcaggaag cggaagactg cggcaatggc agcctaatca aaacagactg atcgaaattt
      181 tgagagaccg cggtcgaggc ggtctctctt tttgcttcgg tgctagcgcg ggggcaaacg
      241 ctcaaatgtc tgcaggccat ccggaatgac gtggaaaggt tgcctgtcga cagtatagat
      301 cgccatttga ggcgtgccga atagatccgg attatcaagc gtgccgacct tcaggatgac
      361 cagcggcaga ccaggtcgtc gcgttgtcag atgagtcccg cattcgccac aaaactcacg
      421 cgtcaccggg ctttcgagat cggaacgcgt gaacttgcgc ggttcaccgc ttgtgtaacg
      481 aaagccttcc ggcgccatga gcatgaacat gttcggcgcg ccaccgctga tgtactggca
      541 ttcacggcaa tggcattgcg ccttgagtcg aggttcgcct tccgccacgt agcgaatttt
      601 gcggcaatag cacccgcctt caaatttcat ggataaatcc tccaaacaaa gtcactgcgg
      661 ctgcgcgctt catataaacc gcatcagcat caacccgccg gtcaccacca cgaagatgac
      721 cgcggtggtg ccgagcacgg tagtgatgtg ccgccagccg aggccgatga tcgtcttcac
      781 cgacgtcccc aggccgagcg cgccgatggc gagcagcagt ccccaggtcg aaatttccac
      841 cagcaggctt tttgccggcg catagaccgg cagccaggac gggacaagcg gcatcgcgct
      901 gttcaggacg cacaacagca ggaagacgat ggcgaagacc ggcaccggca cgcgcgcctc
      961 gccgtgccgc atgcccatgc gcgtgaaatg ccagccgacg gcgagcacga ccggcagcag
     1021 caggaatacg cgaaagagtt tcacgatcac ggccgtgttg ccgaccggct ccgataccgc
     1081 gtaaccggcg ccgacgacct gcgcgacgtc atggatcgtg ccgccgagca tcacgccggt
     1141 tgtctgcgcg tcaaaaccga gcgcaatgca gagcggcgga tagaccagca ttgccagcgt
     1201 ggccagcgca ttcaccgcca ccaccacgaa ggcgatgtcg gccgctttgc ccttgtattc
     1261 gggcacgacc gtcgacgtcg ccagcgtcgc cgaggcaccg cacaccgccg tgccgacccc
     1321 gaccagcgcg ccgaagccgg cgctgcggcc gtaccagcgc gcaaagacga atccggacgc
     1381 aaccgttgcc gccatcgcca tgacgatcag cacggcggtc tgcaggccga gcgcggcgat
     1441 atcgccgagc ccgacgcgca ggccgagcag cgcgacggcc cagcgcaaca gcgtcttcac
     1501 gcagaaattc atgcccggct gcaccgccgg ccgcgcggcg agcggattga gcgcgatgcc
     1561 gacgacgagc gcgatcacca tgttgggaat aggaacgacc ttggctacga agggcgcggc
     1621 gatgtaaccg atggccgcca ccagcgccga cagaagaatg ccgggcgcca gggccgccgc
     1681 catggccacc gacggccgca aggcttgcaa attttctgcg ctcacgcggg atccttattg
     1741 gctggcggcg actttggagc ggccggattg aagacctgcc gcaacagcgg cggcgatttg
     1801 cgagggtgta tcgaccacag tgacgcctgc cgcctcgagc gcctggcgct tggaggcata
     1861 gctgccggca ttgccggtca cgatcgcgcc ggcatgaccc attttcttgc ccgtcggcgc
     1921 ggcggcgccg gcgatgaagg cgaccatcgg cttggccatc gtttttgcgc aggccgccgc
     1981 atcttcttcc atcgcaccgc cgatctcgcc gacgatcacg atcgcatcgg tacggccgtc
     2041 tttatccagc gcctcgagtg catcgcgcgt ggtggtgccg ag
//
LOCUS       DASSRL010000616         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1415495, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000616 DASSRL010000000
VERSION     DASSRL010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415495"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..971)
                     /locus_tag="VFL49_12550"
     CDS             complement(240..971)
                     /locus_tag="VFL49_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912855.1"
                     /translation="MASSITIDNVNAAYGAVRVIEDVSMKVSAGETVALLGTNGNGKS
                     TLMKCVMGIVRPSAGSIVAEIDGEKFDLVGRTTEQIVDLGIALVPEGRRLFPKLTVEE
                     NLLLGASRKKARAQINSNRDFCFDAFPRLAERKSQLAGSMSGGEQQMLALARALMLAP
                     KILLVDEPSVGLAPLLVARTIDAIKQLKDHYQLTVLMAEQNFKQAVRIADRGYVIVHG
                     KIAFEGRSADELNNNDLVRKLYMGM"
     gene            complement(961..1695)
                     /locus_tag="VFL49_12555"
     CDS             complement(961..1695)
                     /locus_tag="VFL49_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912856.1"
                     /translation="MSAILHVQGLSKRFGGFVALDGIDLEVQPGERLGLIGPNGSGKS
                     TLVNCICGTLQNETGSVQFDGHAVDGLAAHERTHRGLARSFQLPRPFPSLTLADNLRI
                     PMLYTVHKRAGTPLHAESIEARCTELLQLVGLANKGSQRPRDLTQVEMRKLELARAMA
                     AEPKLLIADESMAGLSHSEIEDILTLLVKLNESGITIILIEHIMTAVMSFSQRLAVLV
                     SGKKIADGRPQDVVRDPEVVRAYLGE"
     gene            complement(1692..2690)
                     /locus_tag="VFL49_12560"
     CDS             complement(1692..2690)
                     /locus_tag="VFL49_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7912857.1"
                     /translation="MLPFTLLASAVATGLFLIIREPNVEYLFVAGYTIVQYIVLATAW
                     NILGGYTGYVNFGSAAFFAMGAYSTVALVTFANQLDDLVPENLAPMFHLIFPLSIPTL
                     IIIGGIVSGIVGLGMGYLTLRLRGAFFAIATLAMAVVLQTLVVNWEFVGGSGGAYIVR
                     PGEFPGLGSYMQYLFALMLALAVLSIVIARAIERSRLGYGFAAIRDDEMAAEASGVPT
                     LRLKLIATTISGALMGMAGAPFPYYIGYLQPQSAFGLEYAVNSIAMPMIGGTMSWVGP
                     LVGTLLLGTLQQVANVTISSAVNLLLVGLLLIGFVIVAPNGIVGLVQDRLRGRRAK"
BASE COUNT          532 a          833 c          830 g          515 t
ORIGIN      
        1 gtgccgagga ggctagcatg cggtgctttg acgttcacga attgacaatc ccacaaatcg
       61 attaccaacg atgccctgaa tgtgggtcgt ctatgcgact cgaatggatc gagccggata
      121 agaaatctgg ctacgacaag cgtacgttcg agtgcacgca ttgtctgcgc gtcgatacta
      181 tcgtggtgaa gtaccgatga agttaacgcc cgcggcagaa gctgaccggg ttctccgcct
      241 cacattccca tgtaaagttt gcggaccagg tcgttgttgt tgagttcgtc ggccgagcgg
      301 ccttcgaagg cgatcttgcc gtgcacgata acgtagccgc ggtcggcgat gcgcaccgcc
      361 tgcttgaagt tctgctccgc catcaggacc gtgagctgat agtgatcctt gagctgcttg
      421 atggcgtcga tagtgcgcgc gacaagcaat ggcgccagcc cgaccgaagg ctcatccacc
      481 aaaagtatct ttggtgcgag catcagcgca cgcgcaaggg ccagcatctg ctgctcgccg
      541 ccgctcatcg agccggcgag ctggcttttg cgctcggcga ggcgcggaaa ggcgtcgaaa
      601 cagaaatcgc gattgctgtt gatctgcgcg cgcgccttct tacgcgatgc gccgagcagc
      661 agattttctt ccaccgtgag ttttggaaac agccggcggc cttccggtac cagcgcaatg
      721 ccaaggtcga cgatctgctc ggtggtgcgg ccgaccagat cgaatttttc gccgtcgatc
      781 tcggcgacga tgctgcccgc gctgggacga acgatgccca tcacgcactt catcagcgtg
      841 cttttgccgt tgccgttggt gccaagcagg gcgacggtct cgcccgcgct gaccttcatc
      901 gagacgtcct cgatcacgcg caccgcgccg taggcggcgt tcacgttgtc tatggtgatg
      961 ctactcgcca aggtaagccc tcaccacttc cgggtcgcgt acgacgtcct gtggtcttcc
     1021 gtcggcgatc ttcttgcccg ataccagcac cgccaggcgc tgcgagaagc tcatcaccgc
     1081 cgtcataatg tgctcgatca ggatgattgt gatgccgctt tcgttcaact tcaccagcag
     1141 cgtgagaata tcctcgatct cggaatgaga caggccggcc attgattcgt cggcgatcag
     1201 cagcttcggc tcggccgcca tggcgcgtgc cagctcgagc ttgcgcattt ccacctgggt
     1261 cagatcgcgc ggccgttggc ttcctttgtt tgcaagcccg accaattgaa ggagctcggt
     1321 gcatcgcgcc tcgatgcttt cggcgtgcag cggcgttcct gcgcgcttgt gcaccgtgta
     1381 gagcatcggg atgcgcaaat tgtcggccag cgtcaggctg gggaaggggc ggggcagctg
     1441 aaagctgcgc gccagtccgc ggtgcgtgcg ctcatgagcg gccaaaccgt caaccgcgtg
     1501 accgtcgaat tggaccgagc ccgtttcgtt ctgcagcgtt ccgcagatgc aattgaccaa
     1561 ggtgcttttg cccgacccgt ttggcccgat caggccgagc cgctcaccgg gctggacttc
     1621 gagatcgatg ccatcgagcg cgacgaagcc gccgaaccgt ttgctcaggc cttgaacgtg
     1681 caggatggcg ctcattttgc cctccgcccg cgcaggcggt cctgcaccag gccgacaatt
     1741 ccgttaggcg ccacgatcac aaagccgatc agcagcaggc cgaccaaaag caaattgacc
     1801 gcggaggaga tcgtcacatt ggcgacctgt tgcagtgtgc ccagcagcag cgttccgacc
     1861 agagggccga cccagctcat ggtgcctccg atcatcggca ttgcgatcga attgaccgcg
     1921 tattcgaggc caaaggccga ttggggctgc aggtagccga tgtaataggg gaacggtgcg
     1981 ccggccatgc ccatcagcgc gcccgatatc gtggtcgcaa tcagcttcag gcgcagtgtc
     2041 ggcacgccgg acgcctctgc ggccatttcg tcatcgcgga ttgccgcaaa tccgtagccc
     2101 agccgcgagc gctcgatggc gcgcgcgatc acgatcgaga gcaccgcaag cgccagcatc
     2161 agcgcgaaga ggtactgcat gtagctgccg agccccggaa attcgcccgg ccgcacgatg
     2221 tacgctccgc ctgagccgcc gacgaattcc cagttgacca ccagcgtctg cagcacgacc
     2281 gccatcgcca gcgtggcgat tgcaaagaat gctccgcgca gccgcagcgt caggtaaccc
     2341 atgccgaggc cgacgatgcc tgacacgatg ccgccgatga tgatcagcgt gggaatcgac
     2401 agcggaaaga tcagatggaa catcggcgcc agattttccg gaaccaggtc atcgagctga
     2461 ttggcgaagg tcacgagcgc caccgtgctg taggcgccca tagcgaagaa cgccgccgag
     2521 ccgaaattca cataccccgt atagccgcca aggatattcc aggcggtcgc cagaacgatg
     2581 tattgcacga tggtgtagcc ggcgacgaac aggtattcga cgttcggctc gcgaatgatc
     2641 aggaaaaggc cggtcgcgac ggcggatgcg agcagggtga agggaagcat ccgcacggtt
     2701 cagcgtccca
//
LOCUS       DASSRL010000617         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1206011, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000617 DASSRL010000000
VERSION     DASSRL010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206011"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFL49_12565"
     CDS             complement(<1..760)
                     /locus_tag="VFL49_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HET7912858.1"
                     /translation="MTGLSRREILQVGAAAAALAAGAGAFTRAVAQQSLTEAELLKFD
                     AVGNVTLLHVADIHGQLVPVYFREPSVNLGVGEARGQPPHVTGKEFLERFGIPDRSAS
                     AYALTDRDFEALAKSYGRIGGLDRLAAVVKRVRAERGNDKVLFLDGGDTWQGSLGANR
                     SKGQDMVDCMGLLKPDAMTGHWEFTYGEARVKELIDRLGFPFLALNIRDTEWNEPAFE
                     PMKFFEKGGVKIAVLGQAFPYTPVANPRWLMPKWS"
     gene            complement(767..2029)
                     /gene="soxA"
                     /locus_tag="VFL49_12570"
     CDS             complement(767..2029)
                     /gene="soxA"
                     /locus_tag="VFL49_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HET7912859.1"
                     /translation="MTRGGVLAGAAVVAMLAAAFYVAGGPVAAQSKREALLDTKDDAS
                     QRPWKRYAGWPTRDESKYNTLGNLATPPAPKEPRKITGPITGDAANGAKLVADRGRGG
                     SCLACHVMGPAGGANLPGNVAPDLSEIGNAGREDEWLFNYVYDARVYNPDTVMPPWGS
                     HGFFNDQEINDMVAFLKTLKSPAVFKTALDDPSKRPPPVEKRDNLDPIENPGMWAVDK
                     AQELWKQRGPAGFSCNTCHSDPQTAFKTWAASMPKWEPRLNKVLGVEEFVTRHARATT
                     GANWLMETDDNLAMSVYLRFLANGTPIDVDTSSAEAKAAIERGKELSQRKVGQLNMAC
                     TDCHGKVANHWIRGQWLGEPKGQYDHFPTWRTSLLKVWDIRQRFQWCQVNIRADELPP
                     DAKEYGDLELYLASQNEGLKLSVPGIRH"
     gene            complement(2087..2410)
                     /gene="soxZ"
                     /locus_tag="VFL49_12575"
     CDS             complement(2087..2410)
                     /gene="soxZ"
                     /locus_tag="VFL49_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HET7912860.1"
                     /translation="MAEKPRLKIPKEAKKGDVIEIKTLLPHVMESGQRKDKDGKPIPR
                     KIINKFACEFNGKPVFTANMEPAIAANPYIQFHAKVDESGTFKFSWTDDDGTVTTAEE
                     KITVS"
     gene            complement(2440..2895)
                     /gene="soxY"
                     /locus_tag="VFL49_12580"
     CDS             complement(2440..2895)
                     /gene="soxY"
                     /locus_tag="VFL49_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HET7912861.1"
                     /translation="MRQLSRRDALALGASGAALTVTGWSTSASAAAKEAAEQIDKFTG
                     GKTAEKAKVSIELPEIAENGNTVPLSVSVDAPMTADNYVSEILVVADGNPNPGVATFH
                     LSPLTGKAEASTRIRLATTQNIIAVAKTSKGEFFTNQKLVKVTIGGCGG"
     gene            complement(3038..3604)
                     /locus_tag="VFL49_12585"
     CDS             complement(3038..3604)
                     /locus_tag="VFL49_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7912862.1"
                     /translation="MIGRRNLLVGAASLAVLAASRARAEQPILTDDGLYKQSWFLESF
                     LDLADDLEGARREGKRFAIMWELRGCPYCKETHLVNFAQPRIADYVKANFEVLQLNII
                     GSRKVTDFDGQELSEKAMAAKYGVRFTPTFQFFGEGALKDLPPAKREVSRASGYMLPD
                     DFLAMFRYVREKAYEGKSLRDYLKSRPS"
     gene            3710..>4084
                     /locus_tag="VFL49_12590"
     CDS             3710..>4084
                     /locus_tag="VFL49_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912863.1"
                     /translation="MPAWVARVACLVAALLGGLAESRATDLAEFNAAVEAISAHNRIA
                     IGYLRTENVDLASLEIDRMRNDWITFTERFAGSRPDAFEGNPHYSNLFTAVSARLVGV
                     DLMLKTGRLDAARHGLNAVRKDL"
BASE COUNT          739 a         1322 c         1172 g          851 t
ORIGIN      
        1 aagaccactt cggcatcagc cagcgcgggt tggccaccgg cgtataggga aaggcctggc
       61 cgagcacggc gattttcacg ccgccttttt cgaagaattt catcggctcg aaggcgggct
      121 cgttccactc ggtgtcgcgg atattgagcg cgagaaacgg aaaaccgaga cggtcgatca
      181 gctccttcac ccgcgcttcg ccgtaggtga attcccagtg accggtcatc gcatccggct
      241 tgagcagccc catgcagtcg accatgtcct ggcccttgct gcggttcgcg cccagcgagc
      301 cctgccaggt atcgccaccg tcgagaaaca ggaccttgtc gttcccgcgc tccgcacgca
      361 cgcgcttcac caccgccgcc agccggtcga ggccgccgat gcggccatag gatttggcaa
      421 gtgcctcgaa gtcccggtcg gtcagcgcat aggcagaagc agacctgtcc gggatgccga
      481 accgctcgag aaactccttg cctgtaacgt gtggcggctg gcccctcgcc tcgccgacac
      541 cgaggttgac cgaaggctcg cggaaataga ccggcacaag ctggccgtga atgtcggcca
      601 catgcagcag cgtcacgttg ccgaccgcgt cgaacttcag cagctcggct tcggtaagac
      661 tctgctgcgc gacggcgcgc gtgaacgcgc cagcgcctgc cgcgagcgca gcggccgcgg
      721 ccccgacctg aagaatttcc cgacggctaa gtccagtcaa acctgctcaa tgccggatgc
      781 ccggcacgct caacttcaga ccctcgttct gcgacgccag atacagctcc aggtcaccat
      841 actccttcgc gtccggcggg agctcgtcgg ctcgaatatt gacctggcac cactggaaac
      901 gctggcggat atcccagacc ttcagcaggc tcgtgcgcca ggtcggaaaa tgatcgtact
      961 ggcctttcgg ctcgccgagc cattgtccgc gtatccaatg gttcgcgact ttgccgtggc
     1021 agtccgtgca ggccatgttg agctgaccga ccttgcgttg cgacaactcc ttgccgcgct
     1081 cgatcgcggc cttggcctcg gcgctgctgg tgtcgacatc gatcggcgtg ccgttggcga
     1141 ggaagcgcag ataaaccgac atcgccagat tgtcgtccgt ctccatcagc cagttggcac
     1201 ctgtcgtcgc cctggcgtgg cgcgtgacga actcttccac acccagcacc ttgttgagac
     1261 gcggctccca cttcggcatc gacgccgccc aggtcttgaa cgccgtctgc gggtcgctgt
     1321 ggcatgtatt gcatgaaaaa cccgccggtc cccgttgctt ccaaagctcc tgcgccttgt
     1381 cgaccgccca catcccggga ttttcgatcg gatcgaggtt gtcgcgcttc tcaaccggag
     1441 gcggccgctt gctcggatca tcgagcgcgg tcttgaaaac ggccggcgac ttcagcgtct
     1501 tcaggaacgc aaccatgtcg ttgatttcct ggtcattgaa gaagccgtgg ctgccccagg
     1561 gcggcatcac ggtgtcggga ttgtagacgc gcgcatcata gacgtagttg aacagccatt
     1621 cgtcctcgcg gcccgcgttg ccgatctccg aaaggtctgg cgcgacgttg ccgggcaaat
     1681 tggcgccgcc cgccggaccc atcacgtggc aggcgagaca cgacccgccg cggccacggt
     1741 cggccaccaa tttggcgccg ttggcggcgt cgcccgttat cggtccagtg atcttgcgtg
     1801 gctctttcgg cgcgggcgga gtggcgaggt tgccgagcgt gttgtacttc gactcatcgc
     1861 gcgtgggcca accggcataa cgcttccacg gacgctggga tgcgtcgtcc ttggtatcga
     1921 gcagcgcttc ccttttggat tgagctgcaa ccgggccgcc ggcaacataa aaagcggcgg
     1981 caagcattgc gacgaccgca gcgccagcca gaacccctcc acgcgtcatc agatcctccc
     2041 caacggtttg ttttcattgt ccagcacgca aattgcgcgc cgggtgtcag ctcacggtga
     2101 ttttttcctc cgcggtggtg acagtgccgt catcatcggt ccagctgaac ttgaacgtcc
     2161 cactctcatc aaccttggca tgaaactgta tgtaaggatt tgcggcgatc gccggctcca
     2221 tattggcggt gaacaccggc tttccgttaa actcgcatgc gaacttgttg atgatcttgc
     2281 gcgggattgg cttgccatcc ttgtccttgc gctgaccgga ctccatcaca tggggcagca
     2341 gcgtcttgat ctcgatgacg tcgccctttt ttgcttcttt cggaattttg agtcgcggct
     2401 tttccgccat gattgtctcc cgattctctc gttgtctgtt cagccgccgc agccgccgat
     2461 cgtgaccttc accagcttct gattggtgaa gaactcgcct ttactggtct ttgcaaccgc
     2521 aatgatgttc tgggtggtcg ccaggcgaat ccgggtcgac gcctcggcct tgcctgtcag
     2581 cggcgacaga tgaaacgtcg cgacgcccgg gttgggattg ccgtcggcaa cgacaaggat
     2641 ttcactgaca tagttatccg cggtcatcgg cgcatcgacc gacaccgaca aaggcaccgt
     2701 gtttccgttc tcggcaatct cgggaagctc gatcgacacc ttggccttct cggccgtctt
     2761 gccgccggtg aacttgtcaa tctgttcggc ggcttccttg gccgcagccg atgcactcgt
     2821 gctccagccg gttacggtaa gcgccgcacc tgaggcaccg agcgccaagg cgtcgcgacg
     2881 ggatagttgc cgcattcctg ttcctccaat gagcgtctgg gggcaagccc cctgccccac
     2941 ccagcccgga ccataacggt ggcggacgcg ctattcaaat catattcata aattagaata
     3001 tagttctcgg cgatcccgat atcggaggat aagacgttca gcttggacgc gatttgaggt
     3061 agtcgcgcaa actcttgcct tcgtacgctt tttccctcac gtagcgaaac atcgccagaa
     3121 aatcgtcggg cagcatatag ccggacgccc ggctgacttc acgcttggcg ggcggcagat
     3181 ctttcagcgc cccctcgccg aagaactgga atgtcggtgt gaaccgcacg ccgtatttgg
     3241 cggccatcgc cttttcggac agctcctgcc cgtcgaaatc ggtgaccttg cgcgaaccga
     3301 tgatgttgag ctgcagcact tcgaaatttg ctttcacgta gtcggcgatt cgcggctgcg
     3361 cgaaattcac gaggtgtgtc tccttgcagt acggacagcc tctcagctcc cacatgattg
     3421 caaaacgctt cccttccctg cgcgcaccct cgaggtcatc cgcaaggtcg agaaagcttt
     3481 cgagaaacca ggactgcttg tagagcccgt catcggtcag gatcggctgc tcggcgcggg
     3541 cgcgtgacgc cgcaagcacc gccagagatg ccgcaccaac cagtaaattt cgccgcccaa
     3601 tcatggtcgc attccgtaaa ccacgacgaa aatggcgtct cctcgcccgc cgtggtcatg
     3661 cttatattca tagtcccaat ctgaggcgcc tcaaaatccg gtgaacgcca tgcctgcctg
     3721 ggttgcccgc gttgcatgtc ttgttgccgc tcttctcggc ggcctggccg aatctcgagc
     3781 cacggacctg gccgaattca atgcggcggt agaggccatc agtgcgcaca accgcatcgc
     3841 catcggctat ctccggaccg aaaacgtcga cctcgcctcg cttgaaatcg accgcatgcg
     3901 caacgactgg atcacattca ccgagcggtt tgccggcagc cggccggatg cattcgaggg
     3961 aaacccacat tacagcaatc tgttcactgc ggtcagcgcg cggctggtgg gcgtcgacct
     4021 catgctcaag accggccgtc tcgatgccgc gcgtcacggg cttaatgccg tccggaagga
     4081 cctc
//
LOCUS       DASSRL010000618         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_751242, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000618 DASSRL010000000
VERSION     DASSRL010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751242"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..912)
                     /locus_tag="VFL49_12595"
     CDS             complement(196..912)
                     /locus_tag="VFL49_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561 domain-containing protein"
                     /protein_id="HET7912864.1"
                     /translation="MIGVQTHIPTEVHFDFLGMTIPVHWDYHAILMVVVWFVLAPICI
                     ITIRFGKPKPTLTGIHPKVSIKNAVWWWFSVHKYGLIFSISLALAGAIVAVTVSGGFS
                     GSLHSMFGLTTVALGCLQIIGGWLRGKHGGKNYYSADPNDPKTWFGDHYNMTPRRRKF
                     EAYHKTAGYFVYFFAAGAVASGLMQYPMRGLAMFVIAMVLIAFVVSIVLEYKGYRYDG
                     YRAAHGYTMDAPFNKEREFL"
     gene            complement(1009..2079)
                     /locus_tag="VFL49_12600"
     CDS             complement(1009..2079)
                     /locus_tag="VFL49_12600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7912865.1"
                     /translation="MANVWNKYANTAARKHGKPGREVRPKSLTLDMHAHVAVPRVTEI
                     VTPHLKQATDPLVAASTPETQALMAKQAADIRTRMSTTDERFAVMDEMGVDMQLICPA
                     PPQIYYYLPVDVGVQAARALNDGIAEYVGKHKDRLVALGGVPMQDGNEAARELERGMT
                     QLGFKGVEILTNVNGKELSDPAFAPFWKKAEELDALVLIHPTGFTQPQRFARFYFNNV
                     IGNPLDTTMALHYLIFDGVFERHPKLRVLAVHGGGFLGAYSGRIDHAWGARSDAHGDL
                     PVPPTTYLRRNVYFDTVVFTPHQLEALVKTYGGDRIVLGTDYPFDMLEYDPIGHVASV
                     ESLDNTTRTAICDGNAKKMLRI"
     gene            complement(2155..>2568)
                     /locus_tag="VFL49_12605"
     CDS             complement(2155..>2568)
                     /locus_tag="VFL49_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7912866.1"
                     /translation="AARKPDSGSKHAPWTIDTVFGMFPRLNERRNQIAAHLSGGEQQM
                     LAIGRALMGNPRVLLMDEPSEGLAPQIVAEVMATIRKLKESGLSIVLVEQNPKLVFDV
                     ADDIVILNTGRVAVVSTPAQIEKDGVDLRQHLGVF"
BASE COUNT          509 a          759 c          776 g          524 t
ORIGIN      
        1 gtatcatcga gcgcgcacgc catctcgggg cttatctaca ggcgacgcag acgcaagaag
       61 agaaagcacg cgcggggatg cactcaaccg acggcgcggt gattgcggag acggcggtgc
      121 tggatttctt gtatgcgtga ctggcaattg tcgatgcgct ggccagttat agcccagccg
      181 ccgccagtta tcgcgttaca gaaattcgcg ttctttgtta aaaggcgcgt ccatcgtgta
      241 gccgtgcgcg gcgcggtagc cgtcgtaacg gtaccctttg tattccagaa caatggatac
      301 aacgaatgcg atgagaacca tggcgatgac gaacatcgcc agaccgcgca tggggtattg
      361 catcagaccg ctggcaaccg ccccggcagc aaagaaatag acgaaataac ctgcggtctt
      421 gtgataggcc tcgaacttac gccggcgcgg cgtcatatta taatgatcgc cgaaccaggt
      481 tttcggatca ttcggatcgg cggagtaata attttttccg ccgtgcttgc ctctgagcca
      541 gccgccgatg atctgcaaac agccgagcgc gactgtcgtc agaccgaaca tcgaatgcag
      601 ggacccgctg aagccaccgc tgaccgtaac cgcgacaatt gctcccgcga gcgccagact
      661 gatcgaaaat atcagcccgt atttgtggac gctgaaccac caccaaacgg cattcttgat
      721 gctgaccttt gggtggatgc cagtgagcgt cggcttcggt ttaccgaaac ggatggtgat
      781 gatgcagatc ggagccagca cgaaccagac tacgaccatc aggatagcgt gataatccca
      841 atgaaccggg atggtcattc ccagaaaatc aaaatgaact tcggtcggaa tgtgcgtttg
      901 gaccccaatc actgccacct ccgccgtttt tctcaacttt gaattttcgc gccttttgag
      961 cgcggcagag tagatattag cgttaaaaga ccgctgccga tttcgccctc agatccgcag
     1021 catcttcttc gcgttgccgt cgcaaatcgc ggtgcgggtc gtattgtcga gactttcgac
     1081 cgacgcgaca tggccgatcg ggtcgtactc cagcatatca aacggatagt ccgtgccgag
     1141 gacgatgcga tcgccgccgt aggtcttcac cagtgcttcg agctggtgcg gggtgaagac
     1201 gacggtatcg aaatagacgt tccgtcgcag gtacgtcgtc ggcgggaccg gcaggtcgcc
     1261 gtgcgcatcc gagcgcgctc cccaggcatg gtcgatgcgg ccggaatagg cgcccaggaa
     1321 gccaccgccg tgcacggcaa ggacgcggag cttgggatgc cgttcgaaca cgccgtcgaa
     1381 gatcaggtag tgaagcgcca tcgtggtatc gagcgggttg ccgatcacgt tgttgaagta
     1441 gaaacgggcg aagcgctgcg gttgcgtgaa gccggtcggg tggatcagta ccagggcgtc
     1501 gagctcctcg gcctttttcc agaatggcgc aaaagcgggg tcggacagtt ccttgccgtt
     1561 gacgttggtg aggatctcca cgcctttgaa accgagctgc gtcatgccgc gctcgagctc
     1621 cctggccgcc tcgttgccgt cctgcatcgg tacgccgccg agcgcgacca gccgatcctt
     1681 gtgcttgccc acatactcgg cgatgccgtc attgagcgcg cgcgcagcct gcacgcccac
     1741 gtcaaccggc aggtaatagt agatctgtgg cggcgccgga cagatgagct gcatgtcgac
     1801 gcccatctcg tccatgacgg caaagcgctc gtccgtcgtg ctcatgcgcg tgcggatgtc
     1861 ggcggcctgt ttcgccatca gcgcctgggt ttccggcgtc gaggcagcaa ccagcggatc
     1921 ggtcgcctgc ttgagatgcg gggtcacgat ctccgtcacg cggggcaccg cgacatgggc
     1981 gtgcatatcg agggtgaggc ttttcggcct gacttcccgc ccgggcttgc cgtgcttgcg
     2041 cgcagccgta ttggcatatt tgttccagac gttcgccatt gccactccct cgcttgtcat
     2101 cgtccgcgac ggcggacggc ccagtaacga tgtaatgttt gatttcaatc cggcctagaa
     2161 caccccgagg tgttgccgaa ggtcgacacc gtctttttcg atctgtgcgg gcgtggagac
     2221 aaccgcaaca cggccggtat taaggatcac gatgtcatct gccacgtcga acaccagctt
     2281 cggattctgt tcgaccagca cgatcgaaag tccgctttcc ttcagcttgc ggatagtcgc
     2341 catcacctca gcgacgatct gcggcgccag gccctctgac ggctcgtcca tcagaaggac
     2401 gcgcggattg cccatgagcg cgcggccaat ggccagcatc tgctgctcgc cgccggacag
     2461 atgcgccgcg atctggttgc ggcgctcgtt caggcgcgga aacatgccga ataccgtatc
     2521 gatggtccat ggcgcatgct tgctgccgga gtccggcttg cgggcagc
//
LOCUS       DASSRL010000619         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_313880, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000619 DASSRL010000000
VERSION     DASSRL010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313880"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFL49_12610"
     CDS             <1..206
                     /locus_tag="VFL49_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7912867.1"
                     /translation="LAFEVGTTLVAPPLDLCTDNGAMIAWAGCERLALGLTDTLDVAP
                     RARWPLDEIAAAEGAQRRTAAYK"
     gene            227..1213
                     /locus_tag="VFL49_12615"
     CDS             227..1213
                     /locus_tag="VFL49_12615"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7912868.1"
                     /translation="MNFQHIAVLGGGAWGTALALTCARAGRDVMLWEFDAKNAEHLSV
                     KRESRFLPGVKLDERIKVTRDLGETALAEAILLVVPAQAIRSVVSALSKSLSAAIPLV
                     VCAKGIEHGTQKFMTEIIAGCAPKAVPAILSGPSFAADVARGLPTAVTIAAADSDVAV
                     ALAHAINGDAFRPYHSTDVRGVELGGATKNVLAIAAGIVAGRGLGASASAALTTRGFA
                     ELVRFGQAHGAKTETMMGLSGLGDLILTCSTPQSRNFSCGVALAKGEKADTAAHGKLA
                     EGIFTAPALMEMARAKNVDMPISGAVAAVLAGKMNVDEAIESLLTRPLKSEM"
     gene            1215..1637
                     /locus_tag="VFL49_12620"
     CDS             1215..1637
                     /locus_tag="VFL49_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET7912869.1"
                     /translation="MAYWLLKTEPDDYSWSDQVKRGAKGEAWTGVRNFTARRHLGEMK
                     KGDKFFFYHTGDEKQVVGIGEVTRESFPDPTAAKGEPWVAVTTVADKPLPQPVTLAAI
                     KAEPKLKGMALVKYGRLSVQPVTEAEWKLVCRMGGLKA"
     gene            1728..2156
                     /locus_tag="VFL49_12625"
     CDS             1728..2156
                     /locus_tag="VFL49_12625"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HET7912870.1"
                     /translation="MSFTHANIVSILVAAVAAWIFGGVYYTSLSKWWMAAQGKTPEQC
                     KAEEAGKSAVATAAPFVLLFLAEIIMAWVLYGILLHLNMFTVRTGLIAGAFCWLGFVV
                     TTIASNNAFANRKAMLTAIDCVAWLGALLIIGAILGGFGP"
     gene            2300..>2639
                     /locus_tag="VFL49_12630"
     CDS             2300..>2639
                     /locus_tag="VFL49_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HET7912871.1"
                     /translation="MSEKIYDVPAEWRKRAFVDDAKYKEMYDRSVKDPNGFWAEHGKR
                     VDWMKPFSKVKNTSYAPNNVSIKWFEDGTLNVCHNCVDRHLAKRGDQIALLWEGDDPK
                     DDKKLTYKQLH"
BASE COUNT          531 a          774 c          836 g          498 t
ORIGIN      
        1 gtctcgcatt cgaggtcggc acgacgctcg tcgctccgcc acttgatctc tgtaccgaca
       61 acggtgcgat gatagcctgg gccggctgtg aacggcttgc gctcggtctt accgacactc
      121 tggatgtcgc gccgcgcgcc cgctggccgc ttgatgaaat tgcagccgcc gagggcgcgc
      181 agcgccgcac ggcggcctat aagtgactgc gtcgtaggcc gttagtatga acttccaaca
      241 catcgcagtg ctgggtggag gggcctgggg aacggcgctg gcgcttacct gcgcgcgcgc
      301 cgggcgcgat gtaatgctgt gggaattcga tgcgaaaaac gccgaacact tgtcggtcaa
      361 acgcgagagc cggttcctgc ccggcgtgaa actggacgag cgcatcaaag tgacgcggga
      421 tctgggcgag accgcgctcg ccgaagcaat tctgctggta gttcccgcgc aggcgatccg
      481 ctcggtcgtg agcgcgcttt caaaatcatt gtctgccgca attcctctcg tcgtctgcgc
      541 caagggcatc gagcacggca cgcaaaaatt catgaccgag atcattgccg ggtgcgcgcc
      601 gaaagccgtg ccggcaattt tatcggggcc aagctttgcc gccgatgttg cccgcggcct
      661 gccgaccgcc gtcacgatcg ccgcggctga ttccgatgtg gcggttgcgc ttgcgcacgc
      721 catcaacggt gacgcctttc gcccctatca ctcgaccgat gtccgcggcg tggagcttgg
      781 cggcgcaacg aagaacgtgc ttgccatcgc cgccggcatc gtcgccggcc gcggactggg
      841 cgccagcgct tcggcagcgc tgacaacgcg cggctttgcc gagctcgtgc gcttcggtca
      901 ggcccacggc gccaagaccg agaccatgat gggtctttcc gggctgggtg atctcattct
      961 tacatgctcg actccgcagt cgcgcaactt ctcctgcgga gtcgcgttag cgaaaggcga
     1021 aaaagcggac acggccgcgc atggcaaact cgccgaaggg atattcaccg caccggcgct
     1081 gatggaaatg gcgcgtgcaa agaatgtcga tatgccaata tcgggcgcgg tcgccgcggt
     1141 gctggcagga aaaatgaacg tcgacgaggc aatcgagtca ctgctgacga gacctctaaa
     1201 atcggaaatg tgacatggcg tattggttgt tgaagaccga accggatgac tattcgtgga
     1261 gtgaccaggt caagcgcgga gccaagggag aggcttggac cggcgtgcgc aatttcactg
     1321 ctcgccgcca tcttggcgag atgaaaaaag gtgacaagtt cttcttctat cacacaggcg
     1381 acgagaagca ggtcgttggc atcggtgagg tgacgcggga gtcgtttccc gatccgaccg
     1441 ccgcgaaggg cgagccgtgg gtggcggtca ccactgtggc ggacaagccg ctgccgcagc
     1501 cagtgacgct cgcggcgatc aaggccgagc cgaaactcaa aggcatggcg ctggtgaagt
     1561 acggacgcct atcggtgcag cctgtgaccg aggccgaatg gaagctggtc tgcagaatgg
     1621 gtgggcttaa ggcctgaacc gccgacgccg ggcttgtgcc ggctggcgac ctcttttcat
     1681 aatgcgtcgg aggccggatt gccgggtacg gcgaaatgga ggccctcatg agctttaccc
     1741 acgccaatat tgtttcgatt ctcgttgccg ccgtggctgc ctggattttc ggcggggtgt
     1801 actacaccag cctcagcaag tggtggatgg ctgcgcaggg caagaccccc gagcagtgca
     1861 aggccgagga ggccggtaaa tccgctgtcg caacggcggc accctttgtc ctgctgtttc
     1921 tcgccgaaat catcatggcc tgggtgctct acggcatcct cttgcatctc aacatgttta
     1981 cagtgcgcac cggcctcatc gccggagcgt tctgctggct cggcttcgtc gtcaccacga
     2041 tcgcttccaa caatgcattt gccaaccgga aggctatgct cacggccatc gactgcgtgg
     2101 cctggctcgg ggcactgctg attatcggag cgatcctcgg cggcttcggc ccttgatagg
     2161 cggaaaatcc ggcccaaatc cgcgaccaaa gtggcaattt gtccgccttg tcgcgatgaa
     2221 aggcgccgtc gcataatggc gctgacagga aaacgaagcg aaagccggcg gcattagcag
     2281 caggcgggag gaagtgtcga tgtccgagaa aatttacgac gtgccggctg aatggcggaa
     2341 acgcgccttt gtcgacgatg caaaatacaa ggaaatgtat gaccgttcgg tcaaggaccc
     2401 gaacgggttc tgggccgagc atggcaagcg cgttgactgg atgaagccgt tcagcaaggt
     2461 caagaacact tcctacgccc cgaacaatgt ctctatcaaa tggttcgagg acggcaccct
     2521 caacgtctgt cacaactgcg tcgaccgtca tctggccaag cgcggcgatc agatcgccct
     2581 tctctgggaa ggcgacgatc cgaaagacga caagaaactt acttacaagc agttgcacg
//
LOCUS       DASSRL010000620         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_33262, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000620 DASSRL010000000
VERSION     DASSRL010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33262"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFL49_12635"
     CDS             <1..309
                     /locus_tag="VFL49_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912872.1"
                     /translation="HAFSTDSAVLALSAEDVEFLERVAVALVKRGMAAPATMFLESMG
                     PMNFLGSQALHVLSPMIECAFDGKDIERLARLLERRDTLSRLIGLIENTASVQRPSAR
                     "
     gene            354..2135
                     /locus_tag="VFL49_12640"
     CDS             354..2135
                     /locus_tag="VFL49_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HET7912873.1"
                     /translation="MIVATDCGSTTTKAILIEKIGDEYRQTYRGEAPTTVEAPFEDVT
                     RGVLNAIAEIEELSKRKILEGDQIITPCRDAQTGVDIYISTSSAGGGLQMMVTGVVQN
                     MTGESAQRAALGAGAIVIDVLASNDGRLPHEKIERIRSMRPDMILMSGGTDGGAVTHV
                     VEMAEYIAAAEPRPRFGVTYKLPLIYAGNKAAQSQVKKILGEKTALEVTDNIRPVLER
                     ENLAPARNKIHDLFLEHVMQQAPGYKKLMDMAGAPIMPTPAAVGLIMETIAKREHLNL
                     IGVDIGGATTDVFSVFEGAFNRTVSANLGMSYSISNVLAEAGLPNIMRWVPFTIDEQT
                     LRNRIKNKMIRPTTIPQTLDELLIEQAIAREALRLALIHHKSLATGLKGVQQERTISD
                     VFEQQTSGRTLIDMRTLDLIVGSGGILSHAPRRIQSMLMMVDAYEPLGFTMLSVDSIF
                     MMPHLGVLSTINEKAATDVFVRDCMIYLGTCVAPIGQGKDGEPCADYAITFADGRTVK
                     DRLRFGDLKQYPLEPGAKAAITIDPAKSVDAGSGLGVSVRREVQGGVVGVLLDGRGRP
                     LQLPVDPSARVAALKKWFDAVDLYPKG"
BASE COUNT          459 a          665 c          706 g          417 t
ORIGIN      
        1 catgcgtttt ccacggattc ggcggttttg gcgctcagcg ccgaggatgt ggaattcctg
       61 gagcgcgtcg ctgttgcgct ggtcaagcgc gggatggcgg ctccagccac gatgttcctc
      121 gaatccatgg ggcccatgaa cttcctggga agccaggcgc tgcacgtgct gagtcccatg
      181 attgaatgcg cgttcgacgg caaggacatt gagcggctcg cccgcctgct cgaacgccgg
      241 gataccctct cgcggttaat cggcctcatt gagaacacgg cgtcggtgca aaggccatcg
      301 gctcgatgac ggctgcctcg tcccttccgc caaacatcga ccgcccattg aatgtgatcg
      361 tcgccaccga ttgcgggagc acgacgacca aggccatttt gattgaaaag atcggggacg
      421 aataccgcca aacgtaccgg ggggaagcgc cgacgaccgt cgaggcgccc ttcgaggacg
      481 tgacgcgcgg cgtgctgaac gcgattgcgg aaatcgaaga gctttccaag cgcaaaattc
      541 tcgaaggcga ccagattatt acgccatgcc gagacgccca gaccggcgtc gacatctaca
      601 tctccaccag cagcgccggc ggcgggctgc agatgatggt gaccggcgtc gtccagaaca
      661 tgaccggcga aagtgcgcag cgcgcggcgc ttggcgccgg cgcgatcgtc atcgacgtgc
      721 tggcgtccaa cgacggccgg ctgcctcacg aaaagatcga gcgcatccgg tccatgcggc
      781 cggacatgat cctgatgtcc ggcggcaccg acggcggcgc cgtgacgcac gtcgtcgaaa
      841 tggcggaata cattgcggcc gcggagccgc gtccgcggtt cggcgtcaca tacaagctgc
      901 ccttgatcta tgcgggcaac aaagccgcgc aatcccaggt caagaaaatc cttggcgaga
      961 aaacggcctt ggaggtcacc gacaacatca ggccggtgtt ggagagagaa aatctggcgc
     1021 ctgcccgcaa caagatccac gatctgtttc tcgagcacgt gatgcagcag gcccccgggt
     1081 acaaaaagct gatggacatg gcgggcgctc cgatcatgcc gactcctgcc gccgtcgggc
     1141 tgatcatgga aaccattgcg aagcgtgaac acctcaacct gatcggcgtc gacatcggcg
     1201 gcgcgacgac ggatgtgttt tcggtattcg agggggcctt caaccggacg gtcagtgcga
     1261 atctcggcat gtcctacagt atctcgaacg tgctggcgga ggcgggattg cccaacatca
     1321 tgcgttgggt gccctttacg atcgatgaac agacgctgcg caatcgcatc aagaacaaaa
     1381 tgattcgacc cacgaccatt ccccagacgc ttgacgagtt gctgatcgaa caggccattg
     1441 cccgcgaggc cttgcggttg gcgctcattc atcataaatc cttggccacc ggcttgaagg
     1501 gagtccagca ggaacgcacg atttcggatg tatttgagca gcagacgtcc ggccgcacgc
     1561 tgatcgatat gcgcacactg gatttgatcg tcggcagcgg aggaattctc tcccatgccc
     1621 cacgccgcat ccagtccatg ctcatgatgg tcgatgccta cgagccgttg gggtttacca
     1681 tgctgtccgt cgacagcatc ttcatgatgc cgcatctcgg ggtgctctcg acgatcaacg
     1741 agaaggccgc gacggacgtc ttcgtgcggg actgcatgat ctatctgggc acctgcgtgg
     1801 ccccgatcgg ccagggaaaa gacggcgaac cctgcgcaga ttatgccatc acattcgcgg
     1861 acggccgaac cgtgaaggac cggctgcgct tcggcgatct caagcagtat cctctggagc
     1921 ccggcgcgaa ggccgcgatc accatcgatc cggccaagtc cgtcgatgcc gggtcggggc
     1981 tcggagtctc ggtcagacgc gaggtgcagg gtggggtggt cggtgtgttg cttgatggcc
     2041 gcggccggcc gcttcaactg ccggtcgacc cctcggcgcg ggttgcagca ttgaagaaat
     2101 ggttcgatgc cgtggatctg tatccgaagg gataggcagg aactactcgt tgatatgtgt
     2161 tcaagttgct caaaaagcac tccagcaagg ccgcaaggag tgaaggaccc gaggcgtgct
     2221 tgtcttgtac gtcgagggtt ctgagcg
//
LOCUS       DASSRL010000621         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_471541, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000621 DASSRL010000000
VERSION     DASSRL010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471541"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFL49_12645"
     CDS             complement(<1..774)
                     /locus_tag="VFL49_12645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET7912874.1"
                     /translation="MRIRDYSLVGLDAAKAVANGLAAAKWYRTDVPRRQMKELMQRSD
                     GPAIRDTLLWIALFALTGTGGWYFWGSWWCIPFFICYGVLYGSSSDSRWHECGHGTAF
                     KTPWLNDVVYHIASFMIMREPTIWRWSHTRHHTNTIIVGLDPEIVATRPPKLFEIALY
                     FLGVTTVPKALQKLVIHAGGRLTDEEKTFVPELEWHKVYVVARIWLAIHASVIAVALW
                     LGSWLPLMYVGVLPTMYGAWLAVYFGLTQHVGMAEDVLDH"
     gene            1133..1927
                     /locus_tag="VFL49_12650"
     CDS             1133..1927
                     /locus_tag="VFL49_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7912875.1"
                     /translation="MLDSSTRNRNTKAIVTGGAQGIGFAIALQLAAEGCASIAIAGRT
                     ADKGARAVDQLRARGVDALFIQADVSKVEDCRRIVSAATERFGSVNGLVNAAATSARG
                     SLIDTSPELFDRIFNTNVRGPFFLMQGVVRRLLETGTPGSIVNILSMSAHAGQSFLNP
                     YSSSKGALATMTKNVAAAYRAKRIRCNAILPGWMDTPAEDEIQRTFHDAPADWLQKAE
                     ASMPMGQLVKTDQLAILATYMLSPHSGVMTGSLVDYDQNVAGVIGE"
BASE COUNT          387 a          637 c          621 g          371 t
ORIGIN      
        1 atggtccagg acgtcctcgg ccatcccgac atgctgcgtc aggccgaaat agaccgccag
       61 ccaggcgccg tacatggtcg gaagcacgcc gacatacatc aacggcagcc acgatccgag
      121 ccagagcgcg accgcgatca cggaggcgtg gatcgcaagc caaatgcgcg cgaccacata
      181 cactttgtgc cattccagct ccggcacgaa cgtcttctcc tcgtcggtca accttccgcc
      241 tgcgtggatg acgagctttt gcagcgcctt cggtaccgtg gtaacgccaa ggaaatacag
      301 cgcgatctcg aacagcttcg gtggccgggt ggcgacgatc tccgggtcga gcccgacgat
      361 gatggtgttg gtatggtgac gcgtgtggct ccagcgccag atcgtcggct cgcgcatgat
      421 catgaatgac gctatgtgat agacaacatc attcagccag ggcgtcttga aggctgtgcc
      481 atgaccgcat tcgtgccatc ggctgtcgct cgatgagcca tagagcacgc cgtagcagat
      541 gaaaaagggg atgcaccacc aggaccccca gaaataccag ccaccagtgc cggtcaacgc
      601 gaacagtgcg atccacagca gcgtgtcgcg gatggccggc ccgtcgcttc gctgcatcag
      661 ttccttcatc tgcctgcggg gaacgtcggt gcgataccac ttcgccgccg ccaatccgtt
      721 ggccacggct ttcgcggcgt cgaggccgac caggctgtaa tcgcgaatcc gcatgacctt
      781 cgccccctga ccaccgatcc cacggccgag gttcgcgggt cgacaacaac atttgatgct
      841 cgtccacgtc cgggcgggat cgatcaaccg atgatataat ctccgaaccg gccccaatcc
      901 aatcactcct cttgatggat tcggatcacc gtgctgcgtc ggcgaccggc gatcgacggt
      961 gtcgaagccg ccaggttccg cttgtcggtc tggaaatatt attctattcc ttgatcagaa
     1021 cggaattact ggcttgcacc ggccagcggg cgcactggtt gcaatgtccc agcggtggga
     1081 gccgcggcga ccatcgcatc ggatttcgcc ccgtccagca atgcaggaaa caatgctcga
     1141 ctccagcact cggaaccgga acaccaaggc gatcgtcacc ggcggggcgc agggcatcgg
     1201 ctttgccatc gcgctgcagc ttgccgcgga aggatgtgcg tccatcgcca tcgccggccg
     1261 cacggccgac aagggggcgc gagccgtgga tcagctcagg gcccgtggcg tggatgccct
     1321 gttcatccag gcggatgtca gcaaggttga ggattgccgg cgaatcgtgt cagcggcgac
     1381 cgagcgcttc ggttcggtca acgggcttgt gaatgctgcg gcgacgtccg cgcgtggctc
     1441 gctgatcgac acgtcgccgg aactgttcga ccggatcttc aacacgaacg tgcgcgggcc
     1501 gttcttcctg atgcagggcg tggtgcggcg gctgttggaa accggtacgc ccggttcgat
     1561 cgtcaatatc ctgtccatgt cggcgcatgc cgggcaatcg ttcctcaatc cctactcgtc
     1621 gtccaaaggc gcgctggcaa cgatgaccaa gaacgtggcc gcggcctatc gcgccaagcg
     1681 catccgctgc aacgcgatcc tgccgggctg gatggacacg cccgcggagg acgagataca
     1741 gcgcacattc catgatgctc cggccgactg gctgcagaag gccgaagcga gcatgccgat
     1801 gggccagctt gtgaagaccg atcagctcgc gatcctcgcg acctatatgc tgagtccgca
     1861 ttcgggcgtc atgaccggct cgctggtgga ttatgaccag aacgtcgccg gcgtgatcgg
     1921 cgaataggac cgcgggaaac gcggctctgc ctaggtgccg tagcggaaga tcttgctgtc
     1981 cttcgcggcg ttctggaaca gcacctcgtc cttggt
//
LOCUS       DASSRL010000622         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_243843, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000622 DASSRL010000000
VERSION     DASSRL010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243843"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..911
                     /locus_tag="VFL49_12655"
     CDS             36..911
                     /locus_tag="VFL49_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7912876.1"
                     /translation="MKPATFEYVVADSVRKAVMALSEAGGEAKILAGGQSLVPMLNFR
                     LVRPAVLVDINRIPDLAFIEETANTIRVGALTRHHRLETSPLVARHLPVLASAMTHVA
                     HLAIRNRGTIGGSLSHADPAAELPMMALLLNAKLHVVSSSGERTVVASDFFRDALTVD
                     LAEDEMVTEIHFPKLPPGTGWGFEEVARRAGDFALAAVAVTVTVSDGKVAEARIAVTG
                     VGPTPVRAQKAEALLRGAAVDTKLIGHAIEAVRALISPDTDLHASSDYRRHLAGVLTG
                     RAVSAAWKRASKIAA"
     gene            908..1384
                     /locus_tag="VFL49_12660"
     CDS             908..1384
                     /locus_tag="VFL49_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7912877.1"
                     /translation="MTKTRDIRVTVNGKRYERAIEPRLLLADFLRQTLGLTGTHVGCE
                     HGVCGACTVLVDGDSVRACLMFAVQADGCTVETVESLGRIGELSPLQESFHAHHALQC
                     GFCTPGMLMTATDLLKKYPLATDDEIREGLSGNLCRCTGYEHIVKAIRAVKAGATS"
     gene            1381..2142
                     /locus_tag="VFL49_12665"
     CDS             1381..2142
                     /locus_tag="VFL49_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7912878.1"
                     /translation="MKMHLLSGGRLRMRKSVYFPDADRSETIEIPVSCALLRHRQGNV
                     LFDTGCHPSVPDNPQARWGELAKLMVPIMPNGDNVVTGLAGIGLQCGDIDIVVCSHLH
                     PDHCGCNTLFTRASFIIHKREIEAARAPNAKNAGYLAGEWEQSTPAEELEGERDLFGD
                     GRIVLIPLPGHTPGTTGALVALDRSGKFLLASDSVSVRATLDRDVIPRNTWNAAALAR
                     SLAEIRRIQKQGATVLCGHDDAQWQSLRKGADAYD"
     gene            2135..>2500
                     /locus_tag="VFL49_12670"
     CDS             2135..>2500
                     /locus_tag="VFL49_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7912879.1"
                     /translation="MTDAAEISVGLKIVGASVKRTEDPRLLTGLGSYTDDRQVARTLH
                     VAFRRSDQSHARICAIDCNAARAAPGVLAVFTAEDLSDIKPLVASSRMANYYATPILP
                     LASGKVRYVGEPVIGVIAES"
BASE COUNT          452 a          797 c          795 g          456 t
ORIGIN      
        1 tcattagtcg ccggacgggg gcgagttttt cgaccatgaa acccgcaacg tttgagtacg
       61 tggttgccga cagcgtgagg aaggccgtca tggccctgtc ggaggcaggt ggcgaggcca
      121 agattctcgc cggcgggcaa agcctcgtgc cgatgctcaa cttccggctg gttcgacccg
      181 cagtcctggt cgatatcaac cgcattcccg atctcgcctt catcgaggag acggccaata
      241 cgatcagagt gggcgcgctc acgcgtcatc atcggcttga gacatcgccg ttggttgcaa
      301 ggcatctgcc ggttcttgcc tcggcgatga cccacgttgc gcatttggcc atccgcaatc
      361 gtggcaccat cggcggaagc ctgtcgcacg ccgacccggc ggccgaactg cccatgatgg
      421 cgcttctgct caatgcaaaa ttgcacgtcg tctcaagcag cggcgaacgc acggtcgtcg
      481 cgtcggattt ctttcgtgat gcgcttaccg tcgaccttgc cgaagacgaa atggtgactg
      541 aaattcattt tccgaagttg ccgcctggca ccggctgggg cttcgaggaa gtggcgcggc
      601 gcgccggcga tttcgccttg gcggcggttg ccgtaaccgt gacggtgtcc gatggcaagg
      661 ttgcggaagc gcgcattgcg gtgactggcg ttggtccgac gcccgtgcgg gcgcaaaaag
      721 ccgaagcgct gcttcgcggt gcggcagttg acacaaagct gatcgggcac gccatcgaag
      781 ccgttcgtgc cctgatctcg ccggacacgg atctgcatgc gtcgtccgac tatcgacggc
      841 atctcgccgg cgtgcttacg ggcagggccg tcagcgcggc gtggaaacgc gcgagcaaga
      901 ttgccgcatg accaaaacac gcgacatccg ggttaccgtc aacggcaagc gctacgagcg
      961 tgcaatcgag ccgcgcctgc tgctcgccga ttttctgcgc cagacgctgg gcctcaccgg
     1021 aacgcatgtc ggctgcgaac acggcgtttg cggcgcctgc acggtgctgg tcgatggcga
     1081 cagcgttcgc gcctgcttga tgttcgcggt gcaggccgac ggttgcacgg tcgagacggt
     1141 cgaaagcctt ggccggatcg gcgaactcag cccgctgcag gaatctttcc acgcgcatca
     1201 cgcgctgcag tgcggctttt gcacgcccgg gatgctgatg acggcgaccg atcttctgaa
     1261 aaaatatccg ctcgccaccg acgatgagat ccgcgagggc ctctcgggca atctctgccg
     1321 ctgcaccggc tatgagcaca ttgtcaaagc tatccgcgcg gtgaaagccg gagccacgtc
     1381 gtgaaaatgc atcttctttc cggcggccgg ctgcgcatgc gcaagagcgt ctatttcccg
     1441 gatgccgacc gcagcgagac cattgaaatc cccgtgtcct gcgcgctcct gcggcaccgc
     1501 cagggcaacg tgctgttcga caccggctgt catccctcgg tgccggacaa tccgcaggca
     1561 cgctggggcg aacttgcgaa gctcatggtg ccgatcatgc cgaacggcga caacgtggtg
     1621 accggtctcg ccgggatcgg tctgcaatgt ggcgacatcg acatcgtggt ctgctcgcat
     1681 ctgcatccgg accactgcgg ctgcaacacg ctgttcacgc gcgcgagttt catcattcac
     1741 aaaagggaaa tcgaggcggc gcgcgcgccg aatgccaaaa acgccggcta tctcgcgggt
     1801 gagtgggagc aatcgacgcc ggccgaggaa ctcgagggcg agcgcgatct gttcggcgac
     1861 gggcgcatcg tgctgatccc gctgcccggt catacgcctg gcacgaccgg cgcgctggtc
     1921 gcactcgacc gttccggcaa atttctgctc gcttcagatt ccgtgagcgt gcgcgccacg
     1981 ctcgaccgcg acgtcatccc gcgcaatacc tggaacgccg ccgctctggc caggtcgctc
     2041 gccgaaatca gacgcattca gaagcaaggc gcgacggtgc tttgcggcca tgacgacgcg
     2101 cagtggcaaa gcctgcgcaa aggtgccgac gcctatgact gatgccgccg agatcagcgt
     2161 agggctaaag atcgtcggcg caagcgtcaa gcgcaccgag gatccgcgtc tgctgaccgg
     2221 gctcggcagc tataccgacg accggcaggt tgcgcgcact ttgcacgttg ccttccgccg
     2281 cagcgaccaa tcgcacgccc gaatttgcgc aattgactgc aacgcggcgc gcgcagcgcc
     2341 aggcgtcctt gcagtgttca cggcggaaga tctgtccgac ataaagcctc tggtcgcgag
     2401 ctcgcgcatg gcgaattact acgcgacccc gattctgccg ctggcgtccg gcaaggtgcg
     2461 ctacgtcggc gagccggtca ttggtgtcat cgccgaaagc
//
LOCUS       DASSRL010000623         5225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1310857, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000623 DASSRL010000000
VERSION     DASSRL010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310857"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..841)
                     /locus_tag="VFL49_12675"
     CDS             complement(101..841)
                     /locus_tag="VFL49_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET7912880.1"
                     /translation="MNGATHQFGVLIAAHGGRHLDRHSNGSIIRLAQNVAARLSGVPV
                     AVGYINGVPTIDAAIAALAAPHVIVYPLFMADGHFGQTVLHRLVETASEMWPGISITV
                     LPPLGLDPALADLVVGKAASAAATLGAPLQDVTLVLLAHGSTRGRASAAAAEWTAKNA
                     HAHQLFADVRVAFLDEAPSLQAVVVEIKGPLVVVGLFSGDGLHGGGDAVRLMAHLDRQ
                     DAIYVGNAGLFSGLEKLISTAVNSALPR"
     gene            complement(838..2403)
                     /locus_tag="VFL49_12680"
     CDS             complement(838..2403)
                     /locus_tag="VFL49_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HET7912881.1"
                     /translation="MTRGLLNKPALAIAALLTLVAEGGAESDGSGLYVEHCASCHGAG
                     RLGGLGPALLPENLGRLTGSRLAAVIAEGRAATQMPPFADKLAKSDIDALAKHVSTPL
                     PSVPVWGKQEIDASRVVYSQPPPIDRPHYDADPLNLFVVVESGDHHVTVLDGDRFAPL
                     ARFATHFALHGGPKFSPDGRFVFFMSRDGWVTKYDLWTLTTIAEIRGGINSRNIAMSS
                     DGKHIAVANYLPHSLVILSSEDLAVERVIEGRDKNGKSSRISAVYQARPRNSFIAALK
                     DVPEVWEISTDPNAPAVYDGMVHSYEKGMVEALPSSQGLFALRRIEVPEPIDDFFFDP
                     PYRNLIGSAREGHALVVNLTVGRDVKRLEMPGLPHLGSGISWVWDGRPVMATPHLKEG
                     RISIFDLTTWSKIKSLDMPGPGFFMRSHENTPFAWTDGMLGSRKDTLVIIDKRTLAIA
                     RTLTPSPGKTAAHVEFDRSGHFAVVSLWEQDGAIVIYDATTFAEIKRIPMSKPSGKYN
                     VWNKITFSDGTSH"
     gene            complement(2381..3589)
                     /gene="nirJ"
                     /locus_tag="VFL49_12685"
     CDS             complement(2381..3589)
                     /gene="nirJ"
                     /locus_tag="VFL49_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HET7912882.1"
                     /translation="MFRLTHYLRELIEPSLAARTRKPPGPVVIWNLVRRCNLTCKHCY
                     SISGDVDFPGELSTEEVFRVMDDLKAFRVPVLILSGGEPLLRKDIFEITARAKALGFY
                     VGLSSNGTLIDGAIADRIDTAGYDYVGISLDGIGGTHDRFRRKTGAYRAALRGLRLLL
                     DRGVKVGVRFTMTSDNAGELPALLDLVETERFPKFYLSHLVYAGRGNKNRGDDASWAA
                     TRAAMDLLIERGWDWVRAGRDIEIVTGNNDADAVYLLKWVAWNMPQRVPHLRATLAQW
                     GGNSSGVNVANIDNLGNVHPDTMWWHYSLGNVKSRPFSQIWTDLSDPIMAGLKRNPRA
                     VGGRCASCHYFDVCGGNTRVRAMRVSGDPWAEDPGCYLTDMEIGLAGPSPRLMTRPYR
                     GLAHDPRPAE"
     gene            complement(3602..4078)
                     /locus_tag="VFL49_12690"
     CDS             complement(3602..4078)
                     /locus_tag="VFL49_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HET7912883.1"
                     /translation="MLDAFDRRLIAATQAGLPLVAEPYRAIARQLAVAESAVRTRLEE
                     LLASGAIRRIGAIPNHYALGVVANGMSVWDVPDASTAAIGGRLGVLDFVTHCYERRRH
                     LPLWPYNLFAMVHGRSRAEVHEKVAEMARLIGPELRAHEVLFSTRILKKTGLRVPA"
     gene            complement(4081..4533)
                     /locus_tag="VFL49_12695"
     CDS             complement(4081..4533)
                     /locus_tag="VFL49_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7912884.1"
                     /translation="MRVFDPTERAIINGLQGGFPLTPHPFREAGRELGLSEGELIDGV
                     RHLVETGRLSRFGPLWNAEGLGGAFCLCAMAVPSERFDVVADQVNAFPEVAHNYERNH
                     ALNMWFVVSSERPQRIAEVLDAISAQTGLDVHQMPKLREFFVGFRVKV"
     gene            complement(4533..>5225)
                     /locus_tag="VFL49_12700"
     CDS             complement(4533..>5225)
                     /locus_tag="VFL49_12700"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HET7912885.1"
                     /translation="SNDYNLWFVIAAPHTRAVATTIASIRKLTGLPVLDLPLQQAYHL
                     DLGFSLRGIRSPRREKPRDVPDFRPDMTDRKLLAAIEDGLAIVRQPYGDVAESVGLEE
                     SDVIARLKRLADSGVVTRFGCIVRHRALGYTANAMAVWDVPDDDVDAVAATLVRDARV
                     TLCYHRKRLPPEWPYNLYCMVHAKSQDSALAVIDDLNATAGITRNAQSVLFSTRCFKQ
                     RGAVFSDQDRGH"
BASE COUNT         1029 a         1634 c         1641 g          921 t
ORIGIN      
        1 caaggagttg tcaaagcact gtgaagacac cgagattttg tgaaagacag gctgctggaa
       61 ccgcaacacg attttgaaac gcacaaagca taactctagc ctagcgcggc agagcgctat
      121 tcactgcggt tgaaatcaac ttttcaagtc ccgaaaacaa gccggcattg ccgacgtata
      181 tggcgtcctg gcgatcgaga tgcgccatta gtcgcaccgc atcgccgcca ccatgcagtc
      241 catcgccaga gaagaggccg accacaacca aaggaccttt gatttcgaca actaccgctt
      301 gcaatgacgg ggcctcatcg agaaaggcaa cccgcacatc ggcaaatagc tgatgggcgt
      361 gcgcgttttt cgccgtccat tcggccgccg cagccgaggc gcggccacgc gtagagccgt
      421 gcgccagcag aaccagcgtc acgtcctgca acggagcgcc taacgtggcc gcggccgaag
      481 ccgctttgcc gacgacaaga tcggccagcg cggggtcaag gcccaagggc ggcagaaccg
      541 tgatcgagat gccaggccac atttcgcttg ccgtttcgac cagccgatgc agcaccgtct
      601 ggccaaagtg accgtccgcc atgaacagtg ggtagacgat gacatgcggc gcggcgagcg
      661 ccgcgatggc agcatcaatc gtcggcacgc cattaatata gccgaccgcg acgggaacgc
      721 cgctaaggcg cgccgcaacg ttctgggcca ggcggatgat gctgccattg ctgtgacggt
      781 ccaaatggcg accgccatgc gcggcgatca gaacgccaaa ttgatgcgtt gcgccgttca
      841 atggctggtc ccatcggaaa acgtgatctt gttccagaca ttgtacttcc ccgacggctt
      901 cgacatcggg atgcgcttga tctcggcaaa tgtcgtggcg tcgtagatca cgatggctcc
      961 gtcctgctcc cacagtgaga cgacggcaaa gtgtccggag cggtcgaact cgacgtgggc
     1021 ggcggtcttg ccgggtgacg gcgtcagcgt gcgggcaatc gcgagcgtgc gtttgtcgat
     1081 gatgaccagc gtgtccttgc ggctcccgag catgccgtct gtccaggcaa acggcgtgtt
     1141 ctcgtggctg cgcatgaaga agccggggcc cggcatgtcg agcgacttga tcttcgacca
     1201 cgtagtcaga tcgaagatgc tgatcctgcc ctccttgaga tgcggggtcg ccattacggg
     1261 ccgtccatcc catacccagg aaatgcccga tccgagatgc ggcaatcccg gcatctcgag
     1321 gcgcttgacg tcgcgtccaa ccgtcaggtt cacgacgagc gcatggcctt ctcgcgcgga
     1381 gccgatgagg ttgcggtagg gaggatcgaa aaagaagtcg tcgataggct caggcacctc
     1441 gatccgccgc aacgcgaaca agccctgtga cgatggcagc gcctcgacca ttcctttttc
     1501 ataggagtgg accattccgt catagaccgc aggcgcattc ggatccgtcg atatctccca
     1561 aacttccggg acatccttga gcgctgcgat gaaggaattg cgcgggcgtg cctgatagac
     1621 ggcggatatg cgtgacgact tgccgttctt gtccctgccc tcgatcacgc gttcgaccgc
     1681 gaggtcttcg ctcgataaaa tgaccaggct gtgcggaaga tagttcgcga ctgcgatgtg
     1741 cttgccgtcg ctggacatcg cgatattgcg cgagttgatg ccgccgcgaa tctccgcaat
     1801 cgtcgtcaat gtccacaggt cgtacttggt cacccagccg tcgcgcgaca tgaagaacac
     1861 gaaacgaccg tccggcgaga attttgggcc gccatgcagc gcgaagtgcg tggcaaaccg
     1921 cgccagcggt gcgaaacggt caccgtcgag gacggtgacg tggtgatcgc cggactcgac
     1981 gaccacaaac agattgagcg ggtcggcgtc gtagtgcggc cgatcgatgg gcggcggctg
     2041 cgaataaacg acacggcttg cgtcgatctc ctgttttccc cacaccggca ctgacggcag
     2101 cggcgtcgac acatgtttgg ccagcgcatc gatgtcggac ttggccaatt tatctgcgaa
     2161 tggcggcatt tgggttgccg cgcgcccctc ggcgatgacg gcggcgaggc gcgatcccgt
     2221 cagccggccg agattctccg gcaacagcgc cgggcccaga ccgccgagcc gcccggcacc
     2281 atggcaactc gcgcaatgct cgacgtaaag cccgctgccg tcggactccg cgcccccctc
     2341 tgccaccagc gtcaggagcg ccgcaattgc gagtgccggc ttattcagca ggccgcgggt
     2401 catgagcaag ccctcgatat ggtcgcgtca tgagccgggg cgacggaccc gccagcccga
     2461 tttccatgtc ggtcagatag cagccgggat cctcggccca cgggtcgccg ctcacgcgca
     2521 ttgcgcgcac gcgcgtgttg ccaccgcaca catcgaaata atggcaggat gcgcaccgcc
     2581 ccccgaccgc gcgcgggttg cgcttcaacc ccgccatgat cggatcggaa aggtcggtcc
     2641 agatttgcga aaacgggcgg cttttcacgt tgccgagcga gtaatgccac cacatcgtgt
     2701 cgggatggac attgccgaga ttatcgatat tggcgacgtt cactccgctg gaattgcccc
     2761 cccattgggc gagcgttgcg cgcagatgcg gcacgcgctg cggcatgttc caggcgaccc
     2821 atttgagcag gtaaacagca tcggcgtcgt tattgccggt gacgatctcg atgtcacgtc
     2881 ctgcgcgcac ccagtcccag ccccgctcga tcaaaagatc catcgctgcg cgcgttgcgg
     2941 cccagcttgc gtcatcgccg cggttcttgt tgccgcggcc ggcgtagaca aggtgcgaca
     3001 gatagaactt tgggaagcgt tccgtttcga cgagatccag caaggccggc agctcgccgg
     3061 cgttgtcgct ggtcatggtg aaccgcacgc caaccttgac gccgcggtcg agcaacagcc
     3121 tcaagcccct gagcgccgcc cgataggcgc cggtcttgcg ccggaagcga tcatgcgtcc
     3181 cgccgatccc gtcgagcgag atgccgacat agtcgtagcc ggccgtgtcg atacggtcgg
     3241 cgattgcgcc gtcgatgagg gtgccgttgc tggacaggcc gacatagaag cccagggcct
     3301 tggctcgcgc cgtgatctcg aagatgtcct tgcgcagcag tggctcaccg cccgacagga
     3361 tcaggaccgg gacgcggaat gccttgaggt cgtccatgac gcgaaagact tcctcggtcg
     3421 acagctcgcc ggggaaatcg acgtcgcctg aaatcgagta acagtgcttg caggtcaggt
     3481 tgcagcgccg caccagattc cagatcacca ccgggccggg cggcttgcgc gttcgtgcgg
     3541 ccaggctggg ctcgatgagc tcccggagat aatgggtcag acggaacacc ggctcattcc
     3601 tctaggctgg gacccgcaat ccggtcttct taaggatgcg ggtagagaac agaacctcat
     3661 gggcgcgcag ctcgggcccg atcagccgcg ccatttcggc gactttttcg tggacttccg
     3721 cacgcgagcg accgtgcacc atggcaaaca aattgtacgg ccagagcggc agatgacgcc
     3781 ggcgctcgta gcaatgcgtg acaaagtcga gcacgccgag gcggccgccg attgcagcgg
     3841 tcgacgcgtc gggcacgtcc cataccgaca ttccgttggc gacgaccccg agcgcatagt
     3901 ggttcggaat cgcgccgata cggcggatgg cgcccgatgc cagcagctct tcgaggcgcg
     3961 tccgcacggc cgactccgcg accgccagtt gccgggcgat ggcgcgatag ggctcggcaa
     4021 ccaggggaag cccggcctgc gtcgcggcga tcaggcggcg gtcgaaggcg tcgagcatga
     4081 tcagaccttc actcgaaacc cgacgaagaa ctcgcgcaat tttggcatct gatgcacgtc
     4141 cagcccggtt tgggcgctga tcgcgtcgag cacctcggca atccgttgcg ggcgttcgga
     4201 agaaacgacg aaccacatgt tgagtgcatg atttcgttcg tagttgtggg cgacttccgg
     4261 aaaggcgttg acttggtctg caacgacgtc gaaccgctct gacggcaccg ccatcgcgca
     4321 caggcaaaag gcaccgccga gcccctcggc attccagagt ggaccgaagc ggctgaggcg
     4381 gccagtttcc actagatgcc ggacgccgtc gatgagttcg ccctcgctca gccccaattc
     4441 cctgcccgcc tcgcggaacg gatgcggtgt cagcggaaag cccccttgca gcccgttgat
     4501 gatcgcccgc tcggtgggat cgaagacacg catcagtgcc cccgatcctg atctgaaaac
     4561 acggcgccgc gttgcttgaa gcaccgcgtc gaaaacagaa cgctctgcgc gttgcgggtg
     4621 atgccggcgg tggcattcaa atcgtcaatg actgcaagag cactatcttg cgatttggcg
     4681 tgcaccatgc agtagagatt gtacggccat tcgggcggca gccgtttgcg gtggtagcac
     4741 agtgtgacgc gcgcatcgcg cacaagcgtg gcggccaccg catcaacgtc atcgtccggc
     4801 acatcccaga ccgccatggc gttcgccgtg tagcccagcg cgcgatgccg cacgatgcag
     4861 ccgaagcggg tcacgacgcc gctatcggcg agccgcttca gccgcgcgat gacatccgat
     4921 tcctcgaggc cgacgctttc cgcgacatcg ccatagggct gccgcacgat ggcgagccca
     4981 tcctcgattg ccgcgaggag cttccgatcg gtcatgtcag ggcgaaaatc cggcacgtca
     5041 cggggtttct cgcgacgcgg ggagcgtatg ccccggagtg agaaaccgag atcgaggtga
     5101 taagcctgtt gcagcggcag atcgagaaca ggaaggccgg tcagcttccg gatcgatgcg
     5161 atggttgtgg ccacggcccg cgtatgcgga gctgcaatca caaaccaaag attgtaatcg
     5221 ttgga
//
LOCUS       DASSRL010000624         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1363224, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000624 DASSRL010000000
VERSION     DASSRL010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363224"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..664
                     /locus_tag="VFL49_12705"
     CDS             260..664
                     /locus_tag="VFL49_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HET7912886.1"
                     /translation="MSVAGTIAVAGLNAATLRLQVAASNIANASTDGPLADAPNPENL
                     PPAYSALQVNQTENAGGGTSATAANRSPATVATFDPSAPYADNRGMVASPNVDLANEL
                     VATLIARLSYGANAKVIRADAQMSAALFDITA"
     gene            complement(684..1778)
                     /locus_tag="VFL49_12710"
     CDS             complement(684..1778)
                     /locus_tag="VFL49_12710"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET7912887.1"
                     /translation="MGAPLELIQQLDNSIAHSTDERRSAMLRHLTDLFLVGSDAYSGD
                     EVDLIDDVFVRLVTTIEESSRALLAIRLGPVPTAPPKILRLLACDDVIEVASPVLTQA
                     ERLDTDTLIECAKTKNQEHLLAISRRKSLPESLTDVLVERGDQQVVLSTAKNGGARFS
                     GRGFATLVRRSDGDDQLAVCVGARPDLPPELFEKLLDVASEKVRAKLELERKYVKTDI
                     ADIVGQVSASIRTAANDQPLSYAAAQVLAESLHRGGLLTVVKLEEFASAERFEEMVAG
                     LSVMSGVPTAIIERNMRETNTQSLLVLAKAIGLSWDTTRSIMALAAKRYRRSMAGIDQ
                     SMSSFNRLRQSTAQQILDFHRMPTRPTRSN"
     gene            complement(1943..2221)
                     /locus_tag="VFL49_12715"
     CDS             complement(1943..2221)
                     /locus_tag="VFL49_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912888.1"
                     /translation="MTQRIDSRDACLLRKSAVLLAISVALLSAAPALAAPMRCSGEEK
                     VCIAACKKSLDRSSISTCVTNCGLRQSACMKSGCWDSGPQRYCGLLKQ"
     gene            2363..>3003
                     /locus_tag="VFL49_12720"
     CDS             2363..>3003
                     /locus_tag="VFL49_12720"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007696069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7912889.1"
                     /translation="MPAKTPFYITTAISYPNGPPHIGHAYEAIATDAIARFMRLDGYD
                     VYFLTGTDEHGQKMQQTATREGLTARQLRDRNVPRFEAMVKLLNCSNDDFIHTTEERH
                     HRASQAIWQAMEKKGDIYLDKYAGWYSVRDEAYYAEDETHLNEHGKRIATKTGTPVEW
                     VEEESYFFKLSAYQQKLLDLYDSVPDFVLPKERLNEVASFVRGGLQDLSISRTT"
BASE COUNT          621 a          913 c          900 g          569 t
ORIGIN      
        1 cacgaaatca ttgagaccca tgcctttggg cgcgactttg acccgcttga ggctcacagc
       61 catgtcgaac tcctcctcgt tgtaatgctt gttggtgctt agtttaccaa gagccggcga
      121 gggcagggag aggcaatccg cgcgaatgtt gcaaatctcc ggctgcccac tcggccttca
      181 aagcagagcc gatcattccg tcaaatttta gggaattgca gaataatctg gtgtcgaaca
      241 aggcttagga cggaacccca tgagcgttgc gggcacaatt gcggttgcgg gattgaatgc
      301 ggcgaccctg cgcctgcagg tcgccgcaag caatatcgcg aacgcgtcaa cggatggccc
      361 gctggcggac gcgccgaacc cggagaacct tccgccggcc tattcggcgc tgcaagtcaa
      421 tcagacggag aatgcagggg gtggaaccag cgccaccgcc gccaatcgtt cgcctgcaac
      481 ggttgcaaca ttcgacccga gcgcgcctta cgccgacaat cgcggcatgg tagcaagccc
      541 gaacgtcgac ctcgccaatg agctcgtcgc aacgctgatc gcccgtttga gctacggcgc
      601 caacgcgaag gtcattcggg ccgacgcgca aatgagcgcg gcattgttcg acattactgc
      661 ctgatcgaaa tcctgcgccg ttgctagttg ctgcgcgtgg gccgcgtcgg catgcggtgg
      721 aagtcgagga tctgctgcgc ggtcgattgc cggagccggt tgaacgacga catcgattga
      781 tcgattcccg ccatcgagcg ccggtatcgt ttggccgcaa gcgccataat gctgcgcgtt
      841 gtgtcccacg aaaggccgat tgccttcgcc aggacgagca gcgactgcgt gttcgtctca
      901 cgcatgttgc gctcgataat cgccgtcggc acgcctgaca tcacggagag ccccgcaacc
      961 atttcctcga accgctcggc gctcgcaaac tcctcgagtt tcacgacggt gagaagcccg
     1021 ccccgatgca gcgattcagc aagcacctgt gccgcggcat agctgagcgg ctgatcattg
     1081 gccgcagtcc tgatgctggc ggaaacctgg ccaacaatgt cggcaatatc ggtcttgacg
     1141 tatttccgtt cgagctcaag ctttgcccgc actttttcgg acgcgacgtc gagcaacttt
     1201 tcaaacagct caggcggaag atccgggcgg gcgccgacgc agacggcaag ctgatcatcg
     1261 ccgtctgagc gcctgaccag cgtggcaaaa cctctgcctg aaaaccgggc gccgccgttt
     1321 tttgcggtgc tcaacaccac ctgctggtcg ccgcgctcga ccagaacgtc ggtgagggat
     1381 tccggcaatg acttacgacg cgaaattgcc agcaggtgtt cctgattttt ggtcttggca
     1441 cattcaatca gggtatcagt gtcgagccgt tccgcctggg tcagtacggg ggaagcgact
     1501 tcgatgacgt catcgcaggc gagcaggcgc aaaattttcg gaggggccgt tggcacgggg
     1561 ccaagccgga tcgccagcag ggcgcgtgat gattcctcga tcgtagtgac aagccggaca
     1621 aaaacgtcat caatcagatc gacctcgtcg ccggaatatg catccgagcc gacgagaaaa
     1681 agatcggtga ggtgacgcag catcgccgag cgacgctcgt ccgtactatg cgcgatcgag
     1741 ttgtcgagct gctggataag ttcgagggga gcgcccatag gtcctgggaa accgttctgg
     1801 ttggccacgg atctggaatc tgcggttggt taccgtccat ttagattgta cgccaggcgg
     1861 gtttaggaat tcgtaaaggc caaacgccgg tcgccaaaaa tgctgacgga ccggctgtgc
     1921 accgccgagg agggtaggcg tcctattgct tgagaaggcc gcagtatcgt tgcgggccgc
     1981 tgtcccagca gccagacttc atgcacgccg actggcgcag cccgcaattg gtcacgcagg
     2041 tcgatatcga cgagcggtcg aggctctttt tgcaggcggc gatacaaacc ttttcctcgc
     2101 cactgcagcg catcggcgcg gccaaagccg gcgccgccga gagcagggca actgaaatcg
     2161 caagcagcac cgcactcttg cgcaggaggc atgcgtcgcg cgaatcgatc cgttgcgtca
     2221 tgccgatgtc ctagggatgt cgcccgccgg cctcaactcc cgggcttccc ttgcttgccc
     2281 cagcggcttt cgattgatac gtactcttca tcgtcatggc cgggcatttc cggccatcca
     2341 tgtctttgaa ttgaatgggc aaatgccggc caagacacct ttctatatca cgaccgcgat
     2401 ctcgtatccg aacggcccgc cgcacatcgg tcacgcctac gaggcaatcg cgaccgacgc
     2461 catcgcgcgc ttcatgcggc ttgacggcta cgacgtctat ttcctcaccg gcaccgacga
     2521 gcacggccag aagatgcagc agaccgccac gcgcgagggt ctgactgcgc ggcaattgcg
     2581 cgaccgcaac gtgccgcgct tcgaggccat ggtgaagctg ctcaactgct cgaacgacga
     2641 tttcatccac acaacggaag aacgtcatca tcgcgcctcg caagccatct ggcaggcgat
     2701 ggagaagaag ggcgacattt atctcgacaa atatgccgga tggtactcgg tacgtgacga
     2761 agcctattac gccgaggacg agacgcatct caacgagcac ggcaagcgca tcgccaccaa
     2821 gacgggcacg ccggtcgaat gggtcgagga ggagagctac ttcttcaagc tctcggccta
     2881 ccagcagaag ctgctcgacc tctacgacag cgtgccggat ttcgtgctgc cgaaggagcg
     2941 gctgaacgag gtggcgagct tcgttcgcgg cggcctgcag gacctgtcga tctcgcgcac
     3001 cac
//
LOCUS       DASSRL010000625         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1224044, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000625 DASSRL010000000
VERSION     DASSRL010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224044"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFL49_12725"
     CDS             complement(<1..651)
                     /locus_tag="VFL49_12725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET7912890.1"
                     /translation="MKCAIKIAGALVALTIASVAQAQNYPAREVKMIVPFPAGGPSDI
                     VARIAADGMSKHLGHNILIENVGGAGGTLGATRASEAAPDGYTLFAASMGTIIAAPTF
                     YPNLKYDSTKDFEPIGMSANAPAAVSINKNIPVKDLKEFVAWAKQKGPEAKQAFGGVG
                     GTSHMACLLFNQVAGIKPTMVAYRGMGPGMNDLMGGHVDIACEQAIVMAAAIQGEKV"
     gene            complement(718..1728)
                     /locus_tag="VFL49_12730"
     CDS             complement(718..1728)
                     /locus_tag="VFL49_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7912891.1"
                     /translation="MARKRGRGMAACWYGIARTAVIDRAAAWAEIDDGGSAKIMTGVT
                     EIGEGILTVLAQIAAHEMGVKPEHVVIGDNDTARAPEASHAGASRQTYMIGNAVALAS
                     REARKRLIDVMAEVWDVDAEAIRTGNGEIWAESTNHRITMGEAVSTAKNRGVVPVGSA
                     TFGTDTTGLHPVDGSGRPWQAYVFGAQVAEVEVDTVTGEVQVLGIWAAHDVGRAVNPQ
                     GVEGQIEGGIVQALGQGLMEDYKLDGGRTSTSGFAKYILPTALDVPRINSIIIEDPDP
                     IGPLGVKGVGEPAMVPTIPAVMNAIYDAVGVRITSLPATPEKVLMAIREKERSEAMQA
                     AE"
     gene            complement(1733..3610)
                     /locus_tag="VFL49_12735"
     CDS             complement(1733..3610)
                     /locus_tag="VFL49_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7912892.1"
                     /translation="MPDLQFTATINGRKIARSARPHQRLLDFIRDDLNLTGNKEGCGA
                     GECGTCSVFVNGVLMKSCLMPLAKAQGATIETVEALAKTGELSVLQKAFHKTGASQCG
                     YCIPGMVMAATAALRTNPFADREEIKERLGGNICRCTGYQKIFDAVELARDVQNGRLP
                     ANALLEDEAEGGDFIGRNVRRIDTPSKVSGRLKYAGDMTMPGMLHVQVLRSPHAHARI
                     VSIDTSAAEAMDGIEGVITCADVPGEDGFGVFVNDQPVMARDKVRYVGEAVAAVAAES
                     ALITRRALSAIKVEYQPLAAVFDPDEAMRPGAPVLHDYAPDNLTKHIPIRVGEVEKGF
                     AESDLVVEETYSTQPIEHAYLEPEAGLAYVDHDGVVTIVSPDQNITHHRHMLARIIAK
                     PISRVRFIMSPVGGGFGGKEDMIYQGMLALLAMKTHRPVRLVFTREESIISTAKRHPS
                     RTILRMGLMRDGRIRALEMKMVCDGGAYGLSTEGVMRKAAILAAGPYSIPNVKVDTYG
                     IYTNNTPSGAFRSFGALQTAFAVESHLDICAERLGLDPFEIRRINAMRDGALTHTKAR
                     LKSVSLTRALNALEEASGWEAGPPNTRGDTRRDLGREDIRPPCALGARLKAVEKQEAA
                     E"
     gene            complement(3603..4523)
                     /locus_tag="VFL49_12740"
     CDS             complement(3603..4523)
                     /locus_tag="VFL49_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET7912893.1"
                     /translation="MTRGAMLTCDEYISPASLADAFAAMKANRGRYRIIAGATDTLPW
                     ARDGRAGDVHIPLLIDVAKIPELCEISVGDKRVRLGAATPVQRFLDDPALVRALPAMP
                     RCAVWFADDQIRAQATVGGNIVNASPAADATPCLIAHDALVELASVRGRRTMPLREFV
                     TGPNKTALAEGELLVALECDALPEHGGAFEKVGHRRSLVISLVCLAVLIRVDRDGRKI
                     EDARLAIAGIGPRPLRLTEVENCLRGPLNAERLDAAADMPVSLVASRTRQEYRRDVVR
                     GFMLRGLINAAQRAGADPGALAPELEAAYA"
BASE COUNT          817 a         1517 c         1424 g          930 t
ORIGIN      
        1 gaccttctcg ccctggatag ctgcggccat gacaatcgcc tgctcgcagg caatgtcgac
       61 atgcccgccc atcagatcgt tcattccggg gcccatgccg cgataggcaa ccatggtcgg
      121 cttgatgccg gcgacctgat tgaacaacag gcacgccatg tgtgaggtcc cgcccacgcc
      181 gccaaaggcc tgtttcgcct cggggccctt ctgctttgcc caggcgacga actctttcag
      241 gtctttcacc gggatgttct tgttgatgga gacggccgcg ggcgcattgg ccgacatgcc
      301 gatcggctcg aaatccttgg tcgagtcgta tttgagattt ggatagaagg tcggcgccgc
      361 gataatggtg cccatgctgg cggcaaacag cgtatagcca tccggcgcgg cttcggaggc
      421 gcgcgtggca ccgagggtgc cgccggcacc gccgacgttc tcgatcagta tattatggcc
      481 aaggtgtttg ctcatgccat ccgcagcgat gcgcgccacg atgtcgctcg ggccgccggc
      541 cgggaacggc acaatcatct tgacttctcg ggccggataa ttttgcgcct gcgccacact
      601 ggcaatggtc agcgcgacga gcgctccggc gattttaatc gcgcatttca tagcgtcctc
      661 cctcggtgct tattaagttg tgtgtttcga acttctccca gacaaaacaa acaacattca
      721 ctccgcagcc tgcatggctt cgctccgctc tttctcacgg atcgccatca gcaccttttc
      781 gggcgttgcc ggcagtgacg taatgcggac gccgaccgca tcgtaaattg cgttcatgac
      841 agccggaatg gtcggtacca ttgccggttc tccgacgcct ttgaccccaa gtggtcctat
      901 cgggtcggga tcctcgataa tgatcgagtt gatccgcggc acgtcgagcg cggtcggcag
      961 tatgtatttg gcaaagcccg acgtgctcgt gcggccgcca tcgagcttgt agtcttccat
     1021 caacccctgt ccgagagcct ggacgatgcc gccctcgatc tggccttcca cgccttgcgg
     1081 attgaccgcg cggccgacgt cgtgcgcggc ccagatgccg agcacctgca cctcgcctgt
     1141 cacagtgtcg acctcgacct ccgccacttg cgcgccaaaa acgtaagcct gccaaggcct
     1201 tccggagcca tcgaccggat gaagaccagt cgtatcagtg ccgaaagtcg ctgagccgac
     1261 cggcacaacg ccgcggtttt ttgccgtact cacggcctca cccatggtga tgcgatgatt
     1321 tgtgctttcg gcccagattt cgccgttgcc ggtgcggatc gcttcggcat cgacgtccca
     1381 tacctccgcc atgacatcga ttagccgctt gcgcgcctcg cgcgaggcga gtgcgacagc
     1441 attgccgatc atgtaggtct gacggctggc gccggcgtgc gaggcttccg gcgcgcgggc
     1501 cgtatcgttg tcgccgatca ccacatgctc gggctttaca cccatctcgt gcgcagcgat
     1561 ctgcgcgagc acggtgagaa taccctcgcc gatctcggtc acgccggtca tgattttggc
     1621 gctgccaccg tcgtcgatct cggcccaggc ggcggcgcga tcgatgaccg cggtgcgggc
     1681 gatcccgtac cagcatgccg ccatccctcg tccgcgtttg cgggccatac gcctactccg
     1741 ctgcttcctg tttttcgacc gctttgagcc gcgcgccgag ggcgcaaggc gggcggatat
     1801 cctcgcggcc aagatcgcgt cgcgtgtcgc cccgcgtgtt cggtggtccg gcttcccagc
     1861 cggacgcctc ttccagcgca ttgagggcgc gcgttaggct taccgacttc agtctcgctt
     1921 tcgtatgcgt cagcgcgccg tcgcgcatcg cgttgatgcg acgaatttca aacggatcga
     1981 gaccgagccg ctcggcgcag atatcgagat gagattcgac ggcaaaggcc gtctgcaacg
     2041 ctccgaagga gcggaaggcg ccggatggcg tgttattcgt ataaatcccg taggtatcga
     2101 ctttcacgtt cggaatggaa tacggcccgg ccgcgaggat cgcagccttg cgcatgacgc
     2161 cttcggtcga caggccgtag gcgccgccgt cgcagaccat cttcatttcc agcgcgcgaa
     2221 tgcgcccgtc gcgcatcaat cccatgcgca gaatcgtgcg cgagggatga cgcttggccg
     2281 tggaaatgat cgattcctcg cgcgtgaaga ccaggcggac cggacgatgc gttttcattg
     2341 cgagcagcgc cagcatgccc tggtagatca tgtcttcctt gccgccgaag ccgccgccga
     2401 cggggctcat gatgaagcgc accctggaga tcggcttcgc gataatgcgg gccagcatat
     2461 ggcggtgatg cgtgatgttc tggtcgggtg agacgatggt cacgacgccg tcatgatcca
     2521 cgtaagcaag gcccgcctcc ggctcgaggt aggcgtgctc gatcggctgg gtagagtagg
     2581 tctcctcgac aacgagatca ctctcggcga agcccttctc gacttcgccg acgcggatcg
     2641 gaatgtgctt ggtgagattg tcgggcgcgt aatcgtgcaa gaccggagcg cccggccgca
     2701 tcgcctcatc cggatcgaaa accgcggcta atggctgata ttcgaccttg atggcggaca
     2761 gcgcgcgcct ggtgatgagg gcgctttcgg cggcaaccgc cgcgaccgcc tccccgacat
     2821 agcgcacctt gtcgcgtgcc atgaccggct gatcgttgac gaaaacgccg aagccgtcct
     2881 cgccgggcac atcagcgcag gtaatgacgc cttcgatgcc gtccatcgct tccgcggccg
     2941 atgtgtcgat cgagacaatc cgcgcgtggg cgtgtggcga gcgcagtacc tgcacgtgca
     3001 gcatgccggg catcgtcatg tcgccggcat atttaagccg gcccgacacc ttgctcggcg
     3061 tgtcgatgcg cctgacgttc ctgccgatga agtcccctcc ctcggcttca tcctcgagca
     3121 gagcattcgc aggcaggcga ccgttctgca catcacgcgc cagctcgacg gcatcgaaga
     3181 tcttctgata gcccgtgcaa cggcagatat tgccgccaag gcgctctttg atttcctcgc
     3241 ggtcggcgaa tggattggtt cgcaatgcgg cggtcgccgc catgaccatg ccgggaatgc
     3301 agtagccgca ctgactcgcg cccgtcttgt gaaaggcctt ctgcaggaca ctcaattcgc
     3361 ctgtcttcgc aagtgcttcc acggtctcga tggtcgcgcc ctgcgctttg gcaaggggca
     3421 tcaggcacga tttcatcagg acgccattca cgaacaccga acaggtgccg cattcgccgg
     3481 cgccgcagcc ttccttgttg cctgtaaggt tgagatcatc gcggatgaaa tcgagcagac
     3541 gctggtgcgg cctggcgcta cgcgcgatct ttcggccgtt gatcgtggcg gtaaactgga
     3601 ggtcaggcat aggcagcctc cagctcgggc gccaatgcgc caggatcggc gcctgcccgc
     3661 tgcgccgcat tgatcaagcc gcgcagcatg aagccgcgca cgacatcgcg ccggtattcc
     3721 tgccgtgtac gcgaggcaac gagcgatacg ggcatatcgg ccgcagcgtc cagccgctct
     3781 gcgtttagcg gaccgcgcag gcaattctcc acctcggtca gccgtaacgg ccttggccca
     3841 atgcccgcga tggcgagccg cgcatcttcg atcttcctgc catcccgatc gacccggatc
     3901 aagacggcaa gacacaccag tgaaatgacg agcgaccgtc gatgaccgac cttttcgaag
     3961 gcgccgccat gctcaggcaa cgcatcgcat tcgagcgcga caagcaattc gccttccgcc
     4021 agcgcggtct tgttcggccc ggtgacgaat tctctgagcg gcatcgtgcg cctgccgcgc
     4081 acagacgcaa gttcgacgag ggcgtcgtgc gcgatcagac agggcgtcgc atcggcagcc
     4141 ggcgatgcat tcacgatatt gccgccgaca gtcgcctgtg cgcggatttg atcatcggcg
     4201 aaccagaccg cgcagcgcgg catcgccggc aaggcgcgga caagcgccgg atcatcaaga
     4261 aagcgctgaa ccggcgttgc ggcgccgagt ctcacccgct tgtcgccgac cgatatttca
     4321 caaagttcgg gaatttttgc gacgtcgatc agcagcggaa tatgcacgtc cccggcccgg
     4381 ccgtcgcgcg cccaaggcag cgtgtcggtc gcgcctgcaa tgatgcgata gcggccgcga
     4441 ttggctttca tcgccgcaaa ggcgtcggcc agcgaggccg ggcttatgta ctcatcgcac
     4501 gtcagcatcg cgcccctcgt catcccttcc gcgtccggaa gggtcgaaag tcattcggcg
     4561 gcttccttct gggcggcgcc catcttcgcc ttcacaacga ccttgatcgc gtcttcgacg
     4621 cgatccttgg catagcggat tgcggtcgga agctcctcga gcggaaacgt gtgggtgtga
     4681 atcttggt
//
LOCUS       DASSRL010000626         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_51470, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000626 DASSRL010000000
VERSION     DASSRL010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51470"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFL49_12745"
     CDS             <1..278
                     /locus_tag="VFL49_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7912894.1"
                     /translation="CVEGDRMIVGLMRMKAGTGAEPHSHPNEQWIYVLEGTFRANIDG
                     KDINVRPGSVVYIPANTVHAGKATADGDVVFFTVKDASHSLHGIKAA"
     gene            335..1321
                     /locus_tag="VFL49_12750"
     CDS             335..1321
                     /locus_tag="VFL49_12750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7912895.1"
                     /translation="MKRLVAVLFAALVLSAGTAMAQDKYPSRPVKVIVPYAPGGATDI
                     VARIVGDAFQKVTGQPVVVLNRPGAFGMLAIDEMVKAAPDGYTLMIGNVSTNAITPIL
                     YASKMNFDYARSVVAVSNLIDVPAFLLVSTANDFPVKSVPELIAYAKQNPGKVRYGTV
                     GIGSYPHYDMAYFAKRAGDLDMVALPNKNGAAGVIQDMLRGDVQAAFLNVASSAGQIQ
                     AGKFRPLAVVNHARLKEYPDIPTMKEVGYPDVGTVAWNGLFAPAATPKPVLEAVFAAM
                     NKALTSPEATEKLTKQNFNVVPSKSLADAKTWLDGEIKHWQTITTAVKIDTQ"
     gene            1357..1932
                     /locus_tag="VFL49_12755"
     CDS             1357..1932
                     /locus_tag="VFL49_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912896.1"
                     /translation="MAQGETSAWVVSQPCPCTFAIPRYRPARQVKLIETIQPCFALGE
                     PGIRPYPKFTVRYQGKDAAMAHVLTFMTNPDSWKGWKKSPSQGVVKRDWVPTGRIDFA
                     TRFNGNVDDSDQPSEFKLLVEERRIVESITGNENLEIQWRLATLNEAKAVVAQYHKYL
                     SENALIRSVVDETVSLPPPKKMQKIHEGAAA"
     gene            complement(2091..2384)
                     /locus_tag="VFL49_12760"
                     /pseudo
     CDS             complement(2091..2384)
                     /locus_tag="VFL49_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          563 a          712 c          719 g          507 t
ORIGIN      
        1 catgcgtgga aggcgaccgt atgattgtcg gcctgatgcg catgaaggcg ggtaccggcg
       61 ccgagccgca ttcacatccg aacgagcaat ggatttacgt gctggaggga actttccgcg
      121 ccaatatcga cgggaaggac atcaatgtaa ggcccggctc ggttgtctat attcccgcca
      181 atacggtaca tgccggcaag gcgacggcgg atggcgatgt cgtcttcttt accgtcaagg
      241 acgcgtcgca cagcctgcac ggcatcaaag ccgcctgagc atgcttcaca gaaaagaaga
      301 aaattttcta cgcctgtaac cctgggagga ttaaatgaaa cgcctggtcg cggttctgtt
      361 cgctgccctc gtattgagcg cagggacggc gatggcgcaa gacaaatacc cgagccgacc
      421 ggtcaaggtg attgttcctt acgcgccggg aggcgccacc gacattgtcg cgcgtattgt
      481 cggcgacgcg tttcagaaag tcaccgggca gcccgtcgta gttctcaaca ggccgggcgc
      541 cttcggcatg ctggcaatcg acgaaatggt gaaggcggca cccgacggct acacgctgat
      601 gatcggcaac gtctcaacca acgcgatcac gccgatcctc tatgccagca agatgaattt
      661 cgactacgcc cgaagtgtcg tggcggtaag caacctgatc gacgtgccgg ccttcctgct
      721 cgtttccacg gccaatgatt ttccggtgaa atcggtcccc gagcttattg cctacgcgaa
      781 gcaaaatccc ggaaaagtcc gctatggcac cgtcggtatc ggttcttatc cgcactacga
      841 catggcttat ttcgccaagc gcgcgggcga tctcgatatg gtggcgctgc cgaacaagaa
      901 cggcgcagcc ggcgtgatcc aggacatgct gcgcggcgac gtgcaggccg catttctcaa
      961 cgtcgcttcg tccgcgggac agatacaagc cggaaaattc cggccattgg ccgtggtcaa
     1021 tcacgcccgg ctcaaggaat atcccgacat tccgaccatg aaggaagtgg gatatcccga
     1081 cgtcggcacg gtggcctgga atggcttgtt cgcgccggcc gcaacgccga agccggtgct
     1141 ggaagcggtg tttgcggcga tgaacaaggc tctgacgtcg cccgaggcga ccgagaagct
     1201 gaccaagcag aacttcaatg tcgtgccgag caagtcgctt gccgatgcga aaacctggct
     1261 cgacggcgaa atcaagcact ggcagaccat caccaccgcc gtgaagatcg acacgcagta
     1321 aattccgcgc gtctttgggg gcgggatgct ttgttggtgg cgcagggcga aacatccgct
     1381 tgggtggttt cgcagccttg cccctgcact tttgccatcc cccgttaccg cccggccagg
     1441 caggttaagc tcattgaaac catacagcct tgcttcgcgt taggagaacc gggaattcgt
     1501 ccgtatccga aattcacggt tcgctatcag ggaaaggacg cagcaatggc tcacgtgctg
     1561 acattcatga ccaatcccga ttcctggaag ggctggaaaa aatcaccgtc gcaaggtgtc
     1621 gtaaaacgag actgggtgcc taccgggcgc atagacttcg cgacacgttt caatggaaac
     1681 gtcgacgatt ctgatcagcc gagtgaattc aagttgctcg tcgaagaacg gcggatcgta
     1741 gagagcatca ccggaaacga aaaccttgag atccagtggc gactcgcgac gctgaatgaa
     1801 gcaaaagccg ttgtggcgca atatcacaag tatctatcgg aaaatgcgct catcagatca
     1861 gtcgtggacg agacggtatc gttgccgccg ccaaagaaga tgcaaaagat tcacgagggt
     1921 gctgccgcct gattgatcgt tcagtgatag acactatgag gcatccatcg acgtaacagg
     1981 gtacgaacgt ccgattttga ccccgctgat ctgcggggtt ccgcaagctg ttttttaagc
     2041 gccgcagcct cggctgtcgc gcaaatcagt tcaaccttca gaccgtcacg ctgggccttg
     2101 agccgattga tgacgtcggc cctcttgccc aattcgatga gttggccggc ggctttgttt
     2161 ctaagctgct cgatggatat ttccatgcgc cgtgtcgaca tcgcaaattc tgcacgcaac
     2221 aaatccttgt ctgcccgaat ttcggtaacc gattgtggga gagccgcctc cagtcgcctg
     2281 acggtcagcc gcacggcgcg ctcatggacg aggggcacga cggccacggc aatcaggcag
     2341 cccgtcaaca ggccgatgcc aatatacatg gccgattcga tcatatcgct gaaacattgg
     2401 actcactgtt tggttccgtc tcaaaacggc aacgccccgc gcccgaatgt ctgctttttt
     2461 acgctactga ttgcgactga gagctgtcgc gagcggatga t
//
LOCUS       DASSRL010000627         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_576533, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000627 DASSRL010000000
VERSION     DASSRL010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576533"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /gene="hrcA"
                     /locus_tag="VFL49_12765"
     CDS             complement(<1..216)
                     /gene="hrcA"
                     /locus_tag="VFL49_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333992.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7912897.1"
                     /translation="MASHDIPIGATQVSLAALNERSREIFRQIVESYLATGEPVGSRN
                     LSRLLPITLSPASVRNVMSDLEQLGLIY"
     gene            321..1034
                     /gene="rph"
                     /locus_tag="VFL49_12770"
     CDS             321..1034
                     /gene="rph"
                     /locus_tag="VFL49_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7912898.1"
                     /translation="MRPSRRQVDELRAVSLERGVVKYAEGSCFVKFGDTHVLVTASLE
                     ERLPPWLKGQGRGWVTAEYGMLPRATSERTRREASAGKQGGRTVEIQRLIGRSLRSVV
                     DLPALGERQITIDCDVIQADGGTRTASITGAWVALADCLNWMGSRDMIKAEVLRDHIA
                     AVSCGVCKGTAVLDLDYAEDSEADTDANFVMTGSGNIVEIQATAEKIPFSEDQFSALL
                     ALARKGIAKLVSLQKLAVA"
     gene            1041..1676
                     /gene="rdgB"
                     /locus_tag="VFL49_12775"
     CDS             1041..1676
                     /gene="rdgB"
                     /locus_tag="VFL49_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET7912899.1"
                     /translation="MARQLTGTVIIATHNPGKLKEMRELLGPYGIATQSAGELGLPEP
                     EEIGTTFAENARIKALAAARASGTPAFADDSGLVVDALGGDPGIHSARWAGPDKDFRA
                     AMNRIQTLLIQRGAKTPERRRAHFIAALCLAWPDGHVENFEGRVDGVAVWPPRGDKGF
                     GYDPLFRPDGFDVTFGQMSAEEKHGLPPRGRGLSHRARAFMLLAEACLSRH"
     gene            1769..2920
                     /gene="hemW"
                     /locus_tag="VFL49_12780"
     CDS             1769..2920
                     /gene="hemW"
                     /locus_tag="VFL49_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET7912900.1"
                     /translation="MSLTIPFGVYVHWPFCLSKCPYCDFNSHVRHGGIDEARFLRAYE
                     TEIAATAVRAPGRTVSTIFFGGGTPSLMQPSSVQAILDCIARHWTVAPDVEVTLEANP
                     TSVEATRFRGFRTAGVNRVSLGVQALDDGALKELGRLHSAQEALDAVAVARSIFGRYS
                     FDLIYARPQQSLDAWASELKRAIAEAAEHLSLYQFTIEPDTPFFGLYKSGKLPVPDQD
                     LSRDLYDLTQVICADAGLPAYEISNHARPGAECRHNLVYWRGHDYAGIGPGAHGRLTV
                     DGRRVATETEKRPEAWLMRVETNGTGVTVSEKLTVGETADEYLLMGLRLAEGIDLERY
                     NTLSGRPLDPKRVSILREEGAVETTTDGRLRVTRSGFPVLDAVVADLAA"
     gene            complement(2923..>3337)
                     /locus_tag="VFL49_12785"
     CDS             complement(2923..>3337)
                     /locus_tag="VFL49_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HET7912901.1"
                     /translation="ANGVDLKRVQLLGTGLWGDDTRISSEKLLDGGWYPSPEQAGFRN
                     FSGRYRARYGQDPVRTATLAYDAVALVAALVKTQGTQRFSEQVLTNPSGFAGIDGVFR
                     FRPEGTNERGLAVLRVTPSGGQVVSPAPKSFVPGT"
BASE COUNT          648 a         1032 c         1023 g          634 t
ORIGIN      
        1 gtagatgaga ccgagctgct ccagatccga catcacgttg cgcaccgagg cgggtgacag
       61 cgtgatcggt agaaggcgcg acagattccg cgatcccacg ggctcgccgg tcgccagata
      121 gctttccacg atctggcgga aaatctcgcg cgatcgctcg ttgagtgccg ccagactgac
      181 ttgcgttgca ccgattggga tgtcgtggga cgccaaaggc ctcgctcctc gaaaacagcc
      241 aacaattgac cattggggcg gtcttgttca agccgggcgt atggttatgg tgagcgacca
      301 atatcagggg agcaagccgg atgcggccaa gccgccgaca ggtcgatgag ctgcgcgctg
      361 tttcgcttga acgcggcgtt gtgaaatacg ctgagggctc gtgtttcgta aagttcggcg
      421 atacccatgt ccttgttacc gccagcctcg aggagcgcct gccgccgtgg ctcaagggcc
      481 agggccgcgg ctgggtaacc gccgaatatg gcatgcttcc gcgcgccacc tcggaacgca
      541 cacggcgcga ggccagcgcc ggcaagcagg gaggccgcac cgttgaaatt caacgactca
      601 tcggacgctc gctgcgatcg gtggtcgatt tgccggcact cggtgagcgt cagatcacga
      661 tcgactgcga cgttatccag gctgatggcg gcacccgcac ggcctcgatc accggcgcct
      721 gggtcgcgct cgcggactgc cttaactgga tggggtcgcg cgacatgatc aaggcggagg
      781 tcctgcgcga tcatatcgcc gctgtttcct gcggtgtgtg caaaggcacc gcggtccttg
      841 acctcgacta tgccgaagat tcggaggccg ataccgacgc caacttcgtc atgaccggct
      901 ccggcaacat tgtcgagatc caggccactg cggagaagat tccgttcagc gaagatcagt
      961 tttcggcgct cctcgcactt gcccgaaagg gcattgcgaa attggtgtcg ctgcagaaat
     1021 tggccgtagc gtaataaaga atggccagac aactgaccgg caccgtcatt atcgccacac
     1081 acaatcccgg gaagctcaag gagatgcggg agttgctcgg gccatatgga atcgcaactc
     1141 agtctgccgg cgaactcggg cttccggagc ctgaggaaat cggcacgaca ttcgccgaga
     1201 acgcgcgcat caaggcgctt gctgcggccc gtgcaagcgg gacgccggcg tttgccgacg
     1261 actccggatt ggtcgtggac gcactcggcg gtgaccccgg tatccactcc gcgcgctggg
     1321 ccggaccgga caaggatttt cgcgcagcta tgaatcgaat ccaaaccttg ttgatccagc
     1381 gcggtgcgaa gactcctgaa cgaaggcgcg cacacttcat tgccgcactc tgtctcgcct
     1441 ggcccgatgg gcatgttgag aatttcgagg gtcgagtcga tggcgtggcg gtgtggcccc
     1501 cgcgcggcga caagggcttc ggctatgatc cgctgtttcg tccggatgga ttcgacgtca
     1561 ctttcggcca gatgagcgca gaggaaaagc acgggctgcc cccgcgcggc cgcggcctct
     1621 cgcatcgtgc ccgcgcattc atgctattgg cggaagcctg tctttcgaga cattaggcgg
     1681 ttccgatcat tacaatttta atcaactctt gtcggcgaga accgccgcga actggccgcc
     1741 gcgggtagct cgaactatga tgcttaacat gtcgttaacg attcccttcg gggtctatgt
     1801 gcattggccc ttctgtctgt cgaagtgccc ttattgcgac ttcaacagcc acgtccggca
     1861 cggcggtata gacgaggcgc ggttccttcg cgcctatgag accgagatcg cagcaaccgc
     1921 agtgcgcgcg ccgggccgga cggtctcgac gatatttttc ggcggcggta cgccgtcgct
     1981 gatgcaaccg tcatcggtac aggcgatact ggactgcatc gccaggcact ggaccgtcgc
     2041 accggacgtt gaggtgacgc tggaagcaaa tccgaccagc gtggaagcaa cgcgcttccg
     2101 cgggtttcgc accgctggcg tcaatcgcgt ctcactcggc gtacaggccc tcgacgatgg
     2161 ggcgctcaag gagctcggcc gactgcatag cgcgcaagaa gcactcgacg ctgtcgctgt
     2221 agctcgctcg attttcggcc gctactcgtt tgatctcatc tacgctcgtc cacagcaaag
     2281 ccttgatgcc tgggcttccg aactcaagcg ggcgatcgcg gaagccgccg agcacctgtc
     2341 gctctaccaa tttacaatcg aaccggacac gccgttcttt ggtttgtaca aaagcggaaa
     2401 attgcccgtc cccgatcagg atctctcgcg cgatctgtac gatctcacgc aagtgatctg
     2461 cgccgatgca ggcctgcctg cctacgaaat ttccaatcac gcgcggccgg gcgccgagtg
     2521 ccgccacaat ctggtgtatt ggcgcgggca cgactacgcc ggcattggcc cgggcgcaca
     2581 cgggcgtctt accgtcgatg gccgtcgcgt tgcgaccgaa acggaaaagc ggcccgaagc
     2641 ctggctgatg cgcgttgaga cgaacgggac cggcgtgacc gtttcagaaa aactcacagt
     2701 gggcgaaacc gccgacgaat atctgctgat gggcctgcgc cttgcggaag gcatcgacct
     2761 tgagcgttac aacaccctct cagggcgtcc cctggatcca aaacgtgttt cgatcctgcg
     2821 cgaggaaggt gcggttgaaa cgaccacaga cggacgactc cgcgtgacac ggagcggatt
     2881 cccggtgctc gatgcggtgg tcgcggatct cgccgcctaa gctcatgttc ccggtacaaa
     2941 cgatttcggc gccggactga ccacctgtcc gccggacgga gtcacgcgca aaaccgcaag
     3001 gccgcgctcg ttcgttcctt ccggccggaa gcggaaaacg ccgtcgatgc ccgcgaagcc
     3061 cgacgggttg gtgaggacct gttcgctgaa gcgttgcgtt ccctgcgttt tgacaagagc
     3121 cgcgaccagt gcgaccgcat catacgcaag cgttgccgta cgcaccggat cctgtccgta
     3181 acgcgcgcgg taccggcccg agaaattccg gaatcccgcc tgctccggtg acggatacca
     3241 gccgccatca agcaatttct ccgacgagat tctggtatcg tcaccccaca gtccggtacc
     3301 gagcaactgc acacgcttga gatcgacgcc atttgct
//
LOCUS       DASSRL010000628         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_961696, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000628 DASSRL010000000
VERSION     DASSRL010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961696"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..828)
                     /locus_tag="VFL49_12790"
     CDS             complement(475..828)
                     /locus_tag="VFL49_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7912902.1"
                     /translation="MKKTYTGGCHCGKVRYEAQADIGEVISCNCSRCRKIGGLLSAVA
                     ASDFKLLSGENEMTDYQFNKRAIHHPFCRTCGIESYAYGKGPGGKDMVMLNVRCLDGI
                     DAEQFKVKKFDGASL"
     gene            982..1704
                     /locus_tag="VFL49_12795"
     CDS             982..1704
                     /locus_tag="VFL49_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET7912903.1"
                     /translation="MADQKTGLVVTAHPGDFVWRAGGAIALHAKRGYRVKIVCMSYGE
                     RGESQFAWKEPGATLEKVKAGRKDEAERAAKLLGAEIEFFDCGDYPLKLNESHFDRMV
                     DIYRELGPSFVLTHALEDPYNFDHPNAAHFAQETRVVAQAMGHKPDAKYKYAAPPMFL
                     FEPHQPEMCNYRPNLILKIDEVWEQKYKAFQILAAQKHLWGYYERVALNRGIQGSRNT
                     GVPMTYGEAYQRLFPTVEEILK"
     gene            1701..2393
                     /locus_tag="VFL49_12800"
     CDS             1701..2393
                     /locus_tag="VFL49_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HET7912904.1"
                     /translation="MKPVVVRNIKRADTNAIGTLGAVGVSTAHEALGRSGLMKPYMRP
                     IWAGAQIAGPAVTVLAQPGDNWMIHVAVEQCQKGDVLVVGCTADNSDGMFGELLATAL
                     RARGVIGLVIDAGCRDVRPISDMRFPVWSKAISAKGTVKATLGAVNVPVVCAGVNVEP
                     GDIVVADDDGVVVIPKKVAVETAQKAQKRKDDEDGKRKQLEAGTLSLDMYKMREPLAK
                     AGLVYIDHPEDN"
     gene            2650..2841
                     /locus_tag="VFL49_12805"
     CDS             2650..2841
                     /locus_tag="VFL49_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET7912905.1"
                     /translation="MSKVLAFLKDESGATAIEYGLIAAGISVVIITAVQSVGSNLNAT
                     FDTIASKLQSANGGGGSGN"
     gene            3440..3610
                     /locus_tag="VFL49_12810"
     CDS             3440..3610
                     /locus_tag="VFL49_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET7912906.1"
                     /translation="MSKVVAFLKDESGATAIEYGLIAAGISVVIIATVNAVGTQLNAK
                     FQIISDKLAGTP"
BASE COUNT          776 a         1089 c         1116 g          794 t
ORIGIN      
        1 accgagggca tgttcgccga catgcacgcg ctctcgcagg atcagctgat gaacgccgag
       61 ctgacgaagc agctcgcgct caagcacgac accgtgatga tcgagcaaag caaggatggc
      121 cgcggcaagg gtacgatttt gggctgaccg gcaccgccat atttcatcct cagaattatc
      181 aacagcgatt cttcgtcctt gccttcgcaa atggcgggcg tacgcttccc cgcaggtcgc
      241 ccatcttcgg ctctgccgga aacggatggc cgccatgacc ggacggacat gggacgtgag
      301 tgctgcttgc agtattcgtg gcttatgact accggccctg gctctcagta actggtacct
      361 gcgctgcagg cttcggccag cgcgccagga atggatccga gagcttgtga cgggcggacg
      421 acggtctgcc cgttatggct tttgggaggt ccgcgcgcga atccccctcc ccgcttacag
      481 gctcgcaccg tcgaatttct tcaccttgaa ttgctcggcg tcgatgccat ccaggcagcg
      541 cacgttgagc atcaccatgt ctttgccgcc cggccccttg ccgtaggcgt aagattcgat
      601 cccgcacgtc ctgcagaagg gatgatgaat cgcgcgcttg ttgaactgat agtcggtcat
      661 ttcgttctcg cccgacagca acttgaagtc ggatgccgcc acggcggaga gcaacccgcc
      721 gatcttgcgg cagcgcgagc agttgcagga aatcacttcg ccgatgtcgg cttgcgcttc
      781 gtaacgcact tttccgcaat ggcagccgcc ggtgtaggtc tttttcatgg tcactgactc
      841 cttttgtgcc tgacgagcgc gacacgcaac gttgtgccgc catttgccat tgtgagatgc
      901 ctccgtttga ccgtcagcga cggcttgacc gcccgctact caacctagtt taacaagccg
      961 gcaagtaaag gagtggcggg catggcggat cagaagaccg ggcttgtggt gacggcccat
     1021 cccggtgatt tcgtttggcg cgcaggcgga gcgatcgcgt tgcatgcgaa gcgaggctac
     1081 cgcgtcaaaa tcgtctgcat gtcatatggc gagcgcggcg aaagccagtt tgcctggaaa
     1141 gagccgggcg ccacgctgga gaaagtaaag gccggccgca aagacgaggc tgaacgcgcc
     1201 gcaaaactgc tcggcgccga gatcgagttt ttcgactgcg gcgactatcc gctcaagctc
     1261 aatgagagtc atttcgaccg catggtcgat atctaccgcg agcttggtcc gtcgtttgtg
     1321 ctgacacacg cgctcgagga tccatacaat ttcgaccatc ccaatgccgc tcatttcgcg
     1381 caggaaaccc gcgtcgtcgc tcaggccatg ggccataagc ccgacgccaa gtacaaatat
     1441 gcagccccgc cgatgttcct tttcgagccg catcaacccg aaatgtgcaa ctacaggccc
     1501 aacctcattc tgaaaatcga cgaggtctgg gagcagaagt acaaggcatt ccagatcctc
     1561 gccgcacaga aacacctgtg gggctactat gaacgggtcg cgctcaaccg gggtatccag
     1621 ggcagccgca ataccggcgt cccgatgacc tatggcgagg cctatcagcg gctgtttccg
     1681 accgtggagg agattctgaa atgaagcccg tcgtcgtccg caatatcaaa cgcgccgaca
     1741 ccaacgccat cgggacattg ggcgccgtcg gcgtgtccac ggcgcacgaa gctctcggac
     1801 gatcaggttt gatgaaaccc tatatgcggc cgatctgggc cggcgcgcag attgccgggc
     1861 cggcggtcac cgtgctggca cagcctggcg acaactggat gatccatgtt gcggtcgaac
     1921 agtgccagaa aggcgatgtc ctggttgtcg gctgcacggc ggacaacagt gacggcatgt
     1981 tcggtgaatt gctggcaacg gcgttgcgcg cgcggggcgt catcgggctt gtcatcgatg
     2041 ccggttgccg cgacgtgcgg ccgatctcgg acatgcgctt tccggtctgg tcgaaagcca
     2101 tttcagccaa gggcacggtg aaggccacgc tcggcgcggt caacgttccg gtcgtttgcg
     2161 ccggcgtcaa tgtcgaaccc ggtgatattg tggttgctga cgacgatgga gtcgtggtca
     2221 ttccaaagaa agtcgctgtc gagaccgcgc agaaggcgca gaaacgcaag gacgatgaag
     2281 acggcaagcg caagcagctc gaggccggca cgctcagtct cgacatgtac aagatgcgcg
     2341 agccacttgc gaaagccggc ctggtctaca tcgaccatcc ggaggataac tgataacggt
     2401 tgtgcacaat ggcccgttaa cgcgcgaggc cgttgaaaaa tattggcgca aaactcgtca
     2461 tccatttgga tgtcgaatct gtctttgaaa aataatctgt tcttggcgtc gtgcgagaaa
     2521 gtttgcaggc agcgactcgc tcttgtctgc aagcggtccg gatttttgga aattgcacgc
     2581 gcgcgatcga agatgccttt aacagcgcgt cgttagtgtg accgcgttgc agtcacacgg
     2641 agtatgcaga tgtccaaagt gcttgcgttt ctgaaagacg aatccggcgc caccgcaatc
     2701 gagtatggat tgatcgcagc cggaatttcg gttgtgatca ttaccgcagt gcagtcggtt
     2761 ggcagtaacc tcaatgcgac gttcgacacg atcgcgagta agctccagtc ggccaacggc
     2821 ggcggtggca gcggcaacta attacgtccc gcctgcactg acattccctg attgtgcaac
     2881 ttagaagccg cctgaatggt atctcaggtc accgagtagc cggtcgcttg cacgcatcct
     2941 cgcggccttc ggcccccgaa gctggcagac ctgggtggaa gaagccgctg gcaacagcgg
     3001 cttctttttc tttccattca tgccgagggt tcggcttgtt tgagagataa ggacaggcgg
     3061 catgtagatg ttttcgcatc ggcgcgcgat cgggccgacg tggttccctc cagccgtccg
     3121 aacgatttga acgatttagc cctcgtggat aatggcccgt taacgaggcc acgtttttcc
     3181 acggccgcaa tatggctggt ggtgacaccc gacgtcggcg gccctgcaat tcctcctgaa
     3241 attcctgagc gaaacagcgc gcctttgtga tcactgcatc cgggtccgga gttacagtga
     3301 ccgagtttcg ctaacaaatt taagcaaatt ctccagtatc aacgaagctt cttcttaacg
     3361 ggccgccggt atcgtcctgc catcatccac tcagatgata tctaggtttc tcagtagcca
     3421 gctacatgga ggatgcgtga tgtctaaggt tgttgcgttt ctgaaggacg agtccggcgc
     3481 gaccgctatc gaatacggtc tgattgccgc gggcatttct gtcgtgatta tcgccacggt
     3541 gaacgccgtc ggcacgcagc tcaatgccaa gttccaaatc atttcggaca agctcgccgg
     3601 tactccgtaa ttcggcgtct agccgaagcg aagaagccgc cggcaacggc ggcttctttc
     3661 tttttggcag ccttttgaca ttgcgcgagc gctagcggtt ggcggcgctc cagggggcga
     3721 tgcggcggcc gacccatttc agcaatgtgg tcagcgacac gccgagcacc atcag
//
LOCUS       DASSRL010000629         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1626613, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000629 DASSRL010000000
VERSION     DASSRL010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626613"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VFL49_12815"
     CDS             <1..146
                     /locus_tag="VFL49_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7912907.1"
                     /translation="PEFPELHRFLAFWKEKLEGPLHSVRVAHCKLIKPAELRAIGSEF
                     RLH"
     gene            506..880
                     /locus_tag="VFL49_12820"
     CDS             506..880
                     /locus_tag="VFL49_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HET7912908.1"
                     /translation="MARIRFDFGTLTLDAELLETPTARAIAAALPITSSALTWGEEVY
                     FDVPVEVKREADARAVVTPGEVAYWPEGPAIALGFGRTPISQGNETRLASPCNIFARA
                     LGDVKVLGRVKAGAKVAVRALE"
     gene            954..2522
                     /locus_tag="VFL49_12825"
     CDS             954..2522
                     /locus_tag="VFL49_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7912909.1"
                     /translation="MADVFRDDKIFVGKSRKPEYLTLKFANRHGLATGATGTGKTVTL
                     QVLAEGFSRLGVPVFAADIKGDLSGVAAPGEAKDFLVKRATDVGFKYQTDEFPVVFWD
                     LFGEQGHPVRTTVFEMGPLLISRMLDLNDVQEGIINIAFRFADDEFGASDAENKGLVD
                     LKDLRELLNYLSKNAKEIGGKYGNVATATVGSVQRQLLVLENQGGARFFGEPALKLED
                     LMRTDRDGRGIINILAADRLMENPRLYATFLLWLMSELFESLPEVGDLDKPKLVFFFD
                     EAHLLFNEAPKTLLDKIEQVVRLIRSKGVGIYFVTQNPLDVPDKVLAQLGNRVQHALR
                     AFTPRDQKAVRAAAETFRPNPKLDTAKVITELGKGEALVSFLEGNGTPSMVERTLICP
                     PAGRLGPVTAAERKSVIARSPIRGKYDQTIDPESAYEILQGRVQGRAGAPEAAPDSGR
                     KSAEPAPPPPSSGGGWTDVLGGILGGGGRSRRMSTGEVVVKQVARSVASQVGTQVGRA
                     LVRGLLGSLGGGRR"
     gene            complement(2636..3175)
                     /locus_tag="VFL49_12830"
     CDS             complement(2636..3175)
                     /locus_tag="VFL49_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912910.1"
                     /translation="MEALHAWHEFYLLVGTAAATVLALLFVAVSLGAGFLSNRDQAPT
                     RTFMSPVVIHFTSVFFIAAVCLIPSHGPRFFAALIAATAALGIVVSVVTSVQVIRREW
                     TQYVPDYFAYGLLPVAAYVTLLLSGVMIYAGMNYAPEVLAGALLALTIINIRNAWDLT
                     LSMVRHHTDERRSTPDQVN"
BASE COUNT          629 a          971 c         1064 g          577 t
ORIGIN      
        1 tcccggaatt tcccgagctc catcgtttcc tggcgttctg gaaagagaag ctggaagggc
       61 cgctgcactc ggtgcgcgtc gcgcactgca agctgatcaa gccagccgaa ctcagggcga
      121 tcggcagcga atttcgcctg cattgagcgc ggaccccgct ttcagaggac ttcgctcgta
      181 gccgacatga gcggagcgta atgcgggaca ttgtccgcgg atttcgcttg cgctcattcc
      241 ggctgcaaga gatcggattc tgttttcaaa cagccaagcg tcgtcgccgg attttgctcg
      301 ggcgctccgg gcaggcccat cttctttttt gtccctcgcg acttgcgagg ggatggagcg
      361 ccgggtggcg caaccatcag ccgacgcctt gcggcccctc gatcgagcag gacaccctat
      421 gactataatc ctatagtcaa gcgagtgccg cgcattgcgc cgataccagc ttttgcgaca
      481 atagcggtca gaagaggaga aagctgtggc gcgaatccgc ttcgatttcg gcacgctgac
      541 tttggacgcc gagttgctcg agaccccgac ggccagggcc attgccgcgg ctctgccgat
      601 cacctcgtcc gcgctgacat ggggcgagga ggtctatttt gacgtgccgg tcgaggtgaa
      661 gcgcgaagcc gacgcgcgcg ccgtggtcac gcccggcgaa gtcgcctatt ggccggaggg
      721 gccggcaatc gcgctcggct tcgggcgcac gccgatcagc cagggaaacg agacgaggct
      781 ggcgagtccc tgcaacatct tcgccagggc actcggtgac gtgaaggtgc tcgggcgggt
      841 gaaagccgga gcgaaggtcg cggtgagggc gcttgaataa gcggcttggc gggccgaata
      901 tcttttccat gcggatgggt taggctaatc cgcgcaaatc gacgggtgaa gtaatggctg
      961 acgtattccg ggatgacaaa atcttcgtcg gcaaaagcag gaagccggaa tatctcacgc
     1021 tcaagttcgc caaccgccac gggcttgcca ccggcgccac cggcacggga aagaccgtga
     1081 cgctgcaggt gctggccgaa ggcttttccc ggctgggcgt gccggtcttt gccgccgaca
     1141 tcaaaggcga cctgtcgggt gtcgctgcgc ccggtgaggc caaggacttc ctggtcaagc
     1201 gcgccacgga tgtcggcttc aaatatcaga ccgacgaatt tcccgttgtg ttctgggacc
     1261 tgttcggcga gcagggccat ccggtgcgca cgaccgtctt tgagatgggc cccttgctga
     1321 tctcacgaat gctcgatctc aacgatgtgc aggaagggat catcaatatc gcgttccggt
     1381 ttgcggacga cgagttcgga gcgagcgacg ccgagaacaa gggactggtc gacctcaagg
     1441 atctgcgcga gctgctgaac tatctgtcca agaatgcgaa ggagatcggc ggaaagtacg
     1501 gcaacgtcgc gacagcgacg gtcggctcgg tgcagcgcca gcttctggtg ctggagaacc
     1561 agggcggggc caggttcttc ggcgagccgg cgctcaagct cgaagacctg atgcggaccg
     1621 accgcgacgg ccgcggcatc atcaatatcc ttgccgccga caggctgatg gagaacccgc
     1681 ggctctacgc cacgttcctg ttgtggctca tgtcggagct gttcgagtcg ttgccggagg
     1741 tgggcgatct cgacaagccc aagctcgtct tcttcttcga cgaggcgcac ctgctgttca
     1801 acgaagcgcc gaaaactttg ctcgacaaga tcgagcaggt ggtgcggctc atccgctcca
     1861 agggcgtcgg catctatttc gtcacccaga acccgcttga cgtgccggac aaggtgctgg
     1921 cgcaacttgg caaccgcgtc cagcacgcgt tgcgcgcctt cacgccgcgc gatcaaaagg
     1981 cggttcgggc ggcggcagaa acgttccggc cgaacccgaa gctcgatacg gcaaaggtga
     2041 tcaccgagct gggcaaaggc gaggcgctgg tgtcgtttct ggaaggcaac gggacgcctt
     2101 cgatggtcga gcgcacgctg atttgccctc cagccggccg tcttggcccg gtgaccgccg
     2161 cggaacgcaa gtccgtcatc gccaggagcc cgatccgcgg caaatacgac cagaccatcg
     2221 atccggaatc cgcttacgaa atattgcagg gccgcgtgca gggacgcgcc ggcgcaccgg
     2281 aagcggcgcc ggacagtggt cgcaagagcg cggagccggc gcctccgccg ccatcgtcag
     2341 gcggcggctg gaccgatgtg ctcggtggaa ttctcggcgg cggcggccgc tcgcgccgca
     2401 tgtcgaccgg tgaggttgtc gtcaagcagg ttgcgcgctc ggtcgcttcc caggtgggca
     2461 cgcaggtcgg cagggcgctc gtgcgcggat tgcttggaag cctgggcggc ggccggcggt
     2521 agatagaaat ccgttagggc tgaagcagtc tctgcttccg ctcattcccg cgaaagcggg
     2581 aatccagatt tctcggctct gggtcgcggg gacgagcgga taataatgga tcgattcagt
     2641 tcacctgatc aggtgtcgat cgccgctcgt cagtatggtg ccgcaccatg gacaaggtga
     2701 gatcccacgc attgcggata ttgatgatcg tgagcgcaag cagcgcgccc gccagcactt
     2761 cgggtgcata attcattccg gcatagatca tgacgccgga caacagcaat gtcacatagg
     2821 ccgccaccgg cagcagcccg taggcgaaat agtcgggcac gtattgcgtc cactcccggc
     2881 gtatcacctg cacggacgtg accacggaca caacgatgcc gagcgccgcc gttgccgcga
     2941 tcagcgccgc aaaaaaccgc gggccgtgcg acgggatcaa gcagacggcg gcgatgaaaa
     3001 acacgctggt aaaatggata accaccgggc tcatgaacgt gcgcgtcgga gcctggtccc
     3061 tgttgctcag gaaaccggcg ccgagcgaga cggcgacaaa cagcagtgcc agcacggttg
     3121 cggcggcggt gccgacgagc agatagaact catgccacgc gtgcagcgct tccatgccgt
     3181 cagcctcttc cgacgaaggg catcgtgttg gccatcaccg tcatgaacag gacgttggtg
     3241 t
//
LOCUS       DASSRL010000630         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1539212, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000630 DASSRL010000000
VERSION     DASSRL010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539212"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1393
                     /locus_tag="VFL49_12835"
     CDS             146..1393
                     /locus_tag="VFL49_12835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7912911.1"
                     /translation="MRIIGKDQLAERPYGWVIVGVATICLALGFGTNSTVSVFMKPFE
                     EEFGWFRADISLAFTLLTVGCAIGGLAWGGLSDLIGARKVGFFGAFAISFGLIALRWQ
                     GDLWLLYLIYFLIGALGFAPLFAPMVALAGLWFDRRKGFAIGIVTAGGAIGQGVVPYL
                     TRLLINEVGWRDAALYLGLGYLLVLLPLLLLLKPAPVAGHSAEGADRTDGNQWGVPHK
                     ISIAWLCVAGFFCCVCMAVPLVHLIPLGIDLGCSPQTAAGLLLSLMIAGVFGRLFFGW
                     LADRTGGLPAYFLASLAQTSVVFWFTQTKDITVLFQLSVLFGFGFAGVMTCLLICARE
                     AAPLRVSGTAIAIVSTMAWIGMGLGSYQAGYFYDLTGAYVLPYGNAVLGGLVNLTVAG
                     MLFWYHRNSRQMPAIGSKAHVQV"
     gene            complement(1390..2082)
                     /gene="radC"
                     /locus_tag="VFL49_12840"
     CDS             complement(1390..2082)
                     /gene="radC"
                     /locus_tag="VFL49_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7912912.1"
                     /translation="MAESPETRLHYHGHRERLRDRFRDAGAEAVSDYELLELVLFRAI
                     PRRDVKSLAKALIASFGSFPEVIAAPATRLAEVKGLGAAAITELKIIHAAASRLARGQ
                     VRKRPVLSSWSNVIDYCRTTMAFAEKEQFRVLFLDKRNQLIVDEVQQTGTVDHTPVYP
                     REVVKRALELSATALILVHNHPSGDPTPSNADIEMTRTIVDVAKPLGIAVHDHIIVGR
                     DGHASLKGLKLI"
     gene            complement(2145..>2571)
                     /locus_tag="VFL49_12845"
     CDS             complement(2145..>2571)
                     /locus_tag="VFL49_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7912913.1"
                     /translation="VTHEAMMRGIAAIRPGATSGDIGHAIQAYVEAQHMSVVRDFCGH
                     GLGRLFHDEPNIVHVGRPGEGIVLKPGMFFTVEPMINLGRPHVKVLSDGWTAVTRDRS
                     LSAQFEHTVGVTKDGVEVFTFSPAGLHKPPYKKAVAARS"
BASE COUNT          428 a          761 c          796 g          586 t
ORIGIN      
        1 ctcgtaaagc agcctccctc cggcggctct ggcccataaa tcacggcaat ctcaagggca
       61 actcaagcaa tccttcaggt atcgcgcccg caattggggc aatttctcca cgcgggtcca
      121 gtcaaggacc gaggagagcg atactttgcg catcattggg aaagaccaac tcgccgaacg
      181 gccctacggt tgggtcattg tcggcgtggc gacgatctgt ctggcgttgg gattcggcac
      241 caatagcacc gtgtcggttt tcatgaagcc gttcgaggaa gagttcggat ggtttcgtgc
      301 cgacatctcg ctggccttta ccctgctgac cgtcggctgc gcgatcggcg gcctcgcttg
      361 gggtggtctt tccgatctta tcggtgcccg caaggtcggc ttcttcggcg cctttgcgat
      421 ctccttcggc ttgattgctt tgcgatggca gggtgacctg tggctgctct atctgattta
      481 ttttctgatc ggtgcacttg gctttgcccc cttgtttgct ccgatggtcg ccctggcggg
      541 cctttggttc gaccggcgaa aggggttcgc gatcggtatc gtgaccgccg gcggcgcgat
      601 cgggcagggc gtcgtgccct atctcactcg ccttttgatc aacgaagtcg gctggcgtga
      661 tgccgctctc tatctcggcc tgggttatct tctggtcttg ttgccgctgt tgcttctgct
      721 caagcctgcg ccagtcgccg gacattcggc tgaaggagct gatcggacgg acggcaatca
      781 atggggcgtt ccgcacaaaa tcagcattgc ctggctctgt gtcgccggat tcttttgctg
      841 cgtctgcatg gctgtcccgc tggtgcacct gataccgctg ggcatcgatc tcggatgcag
      901 tccgcagacc gccgccggtc ttctgttgtc actgatgatc gctggtgttt ttggccgcct
      961 gttctttggc tggctggctg atcgcaccgg aggcctgccc gcgtatttcc tcgcgtcgct
     1021 ggcacagacc tcggtggtgt tctggttcac gcaaaccaag gacatcacgg ttcttttcca
     1081 attgtcggtc cttttcggat tcggctttgc cggcgtgatg acctgcctgc tgatctgcgc
     1141 ccgcgaagcg gcgccgttgc gagtcagcgg caccgccata gcgatcgtgt caacgatggc
     1201 ctggatcggg atgggactcg gcagttatca ggctgggtac ttctacgatc tcacgggagc
     1261 ttatgtactg ccctacggca acgcggtgct cggcggactg gtcaatctta cggtcgccgg
     1321 tatgttgttc tggtaccatc ggaacagccg tcaaatgcct gccattggct cgaaagcgca
     1381 cgtgcaagtc tagatcagct tgaggccctt gagactcgcg tggccgtcgc gtccgacaat
     1441 aatatggtca tgcaccgcaa tgcccaatgg ctttgcgacg tcgactattg tgcgcgtcat
     1501 ttcaatatca gcgtttgacg gcgtcggatc gccggacggg tgattgtgta cgaggatcag
     1561 cgccgtcgcc gataattcga gcgcacgctt gacgacctcg cgcgggtaga cgggcgtgtg
     1621 atcgaccgtg ccggtttgct gcacttcgtc gacgatcagc tgattgcgtt tgtcgagaaa
     1681 caggacgcga aattgctcct tctcggcgaa ggccatggtg gtgcgacaat agtcgatgac
     1741 gttcgaccac gatgagagca ctggccgctt gcgcacctgc ccacgcgcga ggcggctcgc
     1801 cgcggcatga ataattttca gctcggtgat cgcggccgcg cccaggcctt tgacttccgc
     1861 cagccttgtg gccggcgccg cgatcacttc cgggaacgag ccgaagctcg cgatcagagc
     1921 cttggcaagt gatttgacgt cgcgccgtgg aatagcgcga aacaacacga gctcgagcag
     1981 ctcatagtcg ctgacggctt ccgcgccggc gtcccggaag cggtcacgca gccgctcgcg
     2041 atggccgtga taatgcagtc gggtctccgg gctttccgcc atgaaacgaa actagcgcga
     2101 aagcacatgc cagctgtatc agcggaaagg acgagtccgg acgtttaact tctggcggca
     2161 acagccttct tgtacggcgg tttgtgcagc ccggccggtg aaaaggtgaa gacctcgacg
     2221 ccgtccttgg tgacgccgac cgtgtgctcg aactgtgccg aaagcgagcg gtcgcgggtc
     2281 actgcggtcc agccgtcgga cagtactttc acgtgcgggc ggcccaaatt gatcatcggc
     2341 tcgacggtga aaaacatccc gggtttgaga acgattccct caccggggcg cccgacgtga
     2401 acgatattcg gctcgtcgtg gaacaggcgt ccaagtccgt ggccgcagaa atcacggacc
     2461 acgctcatgt gttgtgcctc aacgtaggcc tggatcgcgt ggccgatgtc gccgctcgtc
     2521 gcgcccggcc ggatcgcggc gatgccgcgc atcatcgcct cgtgcgtgac g
//
LOCUS       DASSRL010000631         5387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_664169, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000631 DASSRL010000000
VERSION     DASSRL010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664169"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFL49_12850"
     CDS             <1..231
                     /locus_tag="VFL49_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7912914.1"
                     /translation="VRITEILTDGAKPSKTAAPRPKREPKPQAQAEAAAAESAPARER
                     TKKPAKAAAKKTAKAPTKGKSKAAAATKRKKK"
     gene            285..557
                     /gene="rpmA"
                     /locus_tag="VFL49_12855"
     CDS             285..557
                     /gene="rpmA"
                     /locus_tag="VFL49_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7912915.1"
                     /translation="MAHKKAGGSSRNGRDSAGRRLGLKSTGGEIVLAGNILARQRGTK
                     WHAGRNVGVGKDHTLFALINGKVQFATKAKGRTYVSVVPMTEAAAE"
     gene            683..1282
                     /locus_tag="VFL49_12860"
     CDS             683..1282
                     /locus_tag="VFL49_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7912916.1"
                     /translation="MTLLEEIPTGRFREASIPVLETKRLALRAARLEDAKAVAALAND
                     RRIAENTARIPHPYQLADAEQFIGGANKDGEAAYLITLRDKTIIGACGIMFHYDDTPE
                     LGYWLGVPYWNKGYATEALHALIDYAFTDLTHDSLQAGARVTNPASRRVLEKCGFQWT
                     GVGLYRINSIKSSAPIDRFRLERRIWSALKTWGRVKKIM"
     gene            1459..2001
                     /locus_tag="VFL49_12865"
     CDS             1459..2001
                     /locus_tag="VFL49_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912917.1"
                     /translation="MAYDLDQFIADCRSTLSRDPGPAGREQVRLNLERLLANKDFIEE
                     HCSDSVPRGLKVLYEDPTLGFQILAHINDKARVSPPHDHGDSWAIYGQATKYTDMIEW
                     EREDDGKNPKQAKLKPVKKYRLTPGKAGIYQDGAIHSIDYPDYARFVRVTGTNLDNID
                     RIRIDLKTGEVEKMYAQRAT"
     gene            complement(2230..3207)
                     /locus_tag="VFL49_12870"
     CDS             complement(2230..3207)
                     /locus_tag="VFL49_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET7912918.1"
                     /translation="MDLTAGFLYLAHALNSVFLSLGSVFSVTSLACALCLAIGIVAIQ
                     RRQRSRPIRWRTIVRALLPKRIWSSRSHQTDIGFMCFGVFLYGLLFGWAALSYQFLSN
                     VLIDQLGAAFGPVTPTTLPDWLSRTIMTAVVFLAFELGYWFHHYLSHRIPFMWEFHKV
                     HHTAEVLTPVTVFRVHPLDTMVYYNILAISMGLANGAMCYMLGDSVYQFTITDKNLLL
                     VVFIHAYVHLQHTHLWVSFRGIMGRIFLSPAHHQVHHSTNPAHFNKNLGNCLAIWDWI
                     FGTLYVPAREREKLTFGVLEAAKVDPHSFSEGMVMPVVRAARHIGASGR"
     gene            3728..5293
                     /locus_tag="VFL49_12875"
     CDS             3728..5293
                     /locus_tag="VFL49_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7912919.1"
                     /translation="MILHCVSASVLRAMLTDGEEMALIDVREELIFSQNHLLWARNVP
                     LSRLELRFARLVPRKSTRVVLCDDDDGLAERAAVILADAGYTDVSRLDGGVAAWEKAG
                     YVTFSGVHVPSKAFGEFVEHDSKTPSVSAAELDAMMRSGTDLKVLDSRPFDEYSRISI
                     PAGINVPGAELVLRVRDIVRSPDTTIVVNCAGRTRSIIGAQSLINAGVTNKVIALRNG
                     TMGWHLAGLTCATGKSDRAPDVSNAGLAWARDAAEKVARRFGVTRVDRAAVERWRQDA
                     ARTTYVFDVREPAEYKAGHYHDAISAPGGQLVQATDIYAGVLGARIVLSDDKEVRALM
                     TASWLTQMGWQEVYVLPEAGSETEDPAPTILGSIPSDAAVEPSKILEIDDVSVIDVST
                     SPHYRRGHIPGAWFAIRSRLDKALPKIPLRQGVILTSEDGVTAALALAEARALTTSPV
                     HWLKGGNAGWAAAGFPLSTAEKMADDPIDAWLKPYEQANDHEAAMNAYLSWEVDLLER
                     IQRDGTTHFLHGR"
BASE COUNT         1160 a         1633 c         1677 g          917 t
ORIGIN      
        1 gtgcgcatca ccgagatcct caccgatggc gcaaagccct caaagacggc ggcgccacgt
       61 cccaagcgcg agccgaagcc gcaggcgcag gccgaggcgg ccgcggcaga aagcgcgccg
      121 gcacgcgaaa ggacgaagaa accggccaag gcggccgcaa aaaagaccgc gaaggctcca
      181 acaaagggca agagcaaggc agctgccgct acaaaacgca aaaagaagtg agatgattcc
      241 ttcacggaat cggtgtacat aaaaagagaa ttcgagagac catcatggcg cacaagaaag
      301 caggcggatc ctcacgcaac ggccgcgact cggcaggccg gcggcttggc ctgaaatcaa
      361 caggcggcga gatcgtgctc gccggcaata tccttgcgcg ccagcgcggc acgaagtggc
      421 acgccggccg taacgtcggt gtaggcaagg atcacactct cttcgcgctc atcaatggaa
      481 aggttcagtt cgcaacgaaa gccaaaggcc gcacctacgt ttctgtcgta ccgatgacgg
      541 aggctgcggc cgaataagac ggtggacaaa tccgaggtcc gccggtcatc cagtcaaacc
      601 ggcggggcct ccgagggctc tgagaggggg agacggatgg accggctccc cctctccgct
      661 ttttcaacct ggagcccgtg ccatgacact gctggaagaa ataccgaccg gaaggtttcg
      721 cgaagccagt atccccgtcc tcgagaccaa gcgccttgcc ttgcgcgcgg cgcgcctcga
      781 ggacgctaaa gcggtcgcag cgctcgccaa cgatcgccgc atcgcggaga acaccgcgcg
      841 cattccgcat ccctatcaat tggcggatgc cgagcaattc atcgggggcg cgaacaagga
      901 tggcgaagcc gcgtacctga tcacacttcg cgacaagacc atcatcggtg cctgcgggat
      961 catgttccac tacgatgata cgcccgagct cggctactgg ctcggcgtgc cgtactggaa
     1021 caagggctac gccaccgagg cgttgcacgc gctgatcgac tacgcgttca ccgacctgac
     1081 gcacgattct ttgcaggccg gggcgcgcgt gaccaacccg gcctcgcgcc gggtgctgga
     1141 gaagtgcggc ttccagtgga ccggcgtcgg cctctaccgg atcaactcga tcaaatcatc
     1201 ggcgccgatc gatcgattcc ggctcgagcg gcgaatctgg tcggcgctca agacctgggg
     1261 ccgcgtgaag aaaataatgt agggccaaaa ggctcgcgtc tcgctaccgc tcatgcccgc
     1321 gcatagccgt ctgaaagacg gcgtgcttcc gcacgcctat tgcgggcatc cagcgcgcct
     1381 tcgcaaaggc tgggtccccg ctttcgcggg gacgagcgga tgttaaacta accgcagcct
     1441 atgcccggga gggacgccat ggcttatgac ctcgaccagt tcatcgccga ctgccgttcg
     1501 actctttcgc gcgaccccgg ccctgcgggg cgcgagcagg tgcgtctgaa tctcgaacgt
     1561 ctgctcgcca acaaggattt catcgaagaa cattgcagtg acagcgtgcc gcgcgggctc
     1621 aaagtccttt acgaagaccc gacgctcggt ttccagattc ttgcgcatat caacgacaag
     1681 gcgcgagttt cgccaccaca tgaccatggc gactcatggg cgatctatgg ccaggccacc
     1741 aagtacaccg acatgatcga atgggaacgc gaagacgacg gcaagaaccc caagcaggca
     1801 aagctcaagc ccgtgaaaaa gtaccgtctt acacccggca aggcgggcat ctatcaggac
     1861 ggagccattc actcgattga ttatcccgac tatgcgcgct tcgtgcgcgt gaccggaacc
     1921 aacctcgaca acatcgaccg catcaggatc gacctgaaaa ccggtgaggt cgaaaaaatg
     1981 tacgcgcagc gggcgacgtg actgaacgcg caaatgtttt tctgtcatgg ccggggctca
     2041 ctgagggaag cacagtgccg gcctaagctt gatgcgcgag ccttcgcgcg ccaaaggcgt
     2101 cgtcgggcgc ttaatcacaa caagcgcttc ggcccgcagg cagggcgaac ccattccggt
     2161 gtcgcgcaga tcagcccgcg ttgtcctgac gcagattaag gcgttttgga ttgtcagcgt
     2221 atcgtggtgt caccgaccgg aggcaccgat atgccttgcg gcgcggacga ccggcataac
     2281 catgccttcg ctgaagctgt gtgggtcgac cttcgccgct tcgaggacgc cgaaggtcag
     2341 cttctcgcgc tccctggccg ggacatagag cgtgccgaag atccagtccc agatcgcgag
     2401 gcaattgccg aggttcttgt tgaaatgagc agggttggtg gagtgatgca cctgatgatg
     2461 cgccgggctg aggaagatgc gccccataat gccgcggaag gatacccata ggtgcgtgtg
     2521 ctgcagatgc acataggcat gaatgaagac cacgagcagc aggttcttgt cggtgatggt
     2581 gaactgatag acggagtctc cgagcatgta gcacattgcg ccattggcga gacccatgct
     2641 gatcgcaagg atgttgtagt agaccatcgt gtcgagcgga tgcacgcgga agactgtcac
     2701 cggcgtcagc acctcggcgg tgtgatgcac cttgtggaat tcccacatga agggaatccg
     2761 gtggctcaag taatgatgga accagtagcc gagctcgaag gcgagaaaga cgacggctgt
     2821 catgatcgtg cgtgacagcc aatccggaag cgtcgtcgga gtgacagggc cgaaggccgc
     2881 accgagctga tcgatcagga cattgctgag gaattggtag gaaagcgcgg cccaaccgaa
     2941 taacaaaccg tagagaaaca cgccaaagca catgaaaccg atatcggtct ggtgcgagcg
     3001 gctcgaccag atgcgttttg gaagcagcgc ccggacgatc gtacgccaac ggatcgggcg
     3061 gctgcgttgc cgccgctgga tcgcgacaat cccgattgca aggcagagtg cacaggccag
     3121 cgaggtgacc gaaaagaccg acccaagcga caggaagacg ctgttcagcg cgtgcgcgag
     3181 gtacaagaag cctgccgtaa ggtccaagag cccgctccgt ccgacaaacg cgctcgtgtc
     3241 cttcgaatgc gaagttcgat tgccgaactt gctgccagat cgcgcgggct ttggattcga
     3301 aggacacgag cgcggcgatt tgaagcgata gcctgactgc gtaaatgttc cattatgagg
     3361 ttgctccaga ttcgacggaa acagatgatt tgccccattc aggggcgcat ttgggctccc
     3421 cctagggaga agttgcagtc cttcgccacg gctgggtggt cgcgcttccg ctgaatctcg
     3481 ataagatcga ccggatcagg atcgatctga atgcaggtga ggcaaaaaaa tgtacgcgca
     3541 gcggacgacg tgacttcgtg ccgcgaccgt tgcagcgtca tgcccggcac tcgggtccgg
     3601 ccttcggccg gcccgagcac aggcctcgtg ccgggcatcc acgtcttttc aggtcgccaa
     3661 gtttgtaaga cgtggatggc cgggacgagc ccggccatga cgcggtgaga actctgtgtc
     3721 aacgcgaatg atcctccact gcgtctccgc ctctgtcctc cgggccatgc tcaccgacgg
     3781 cgaggagatg gcgcttatcg atgtgcgcga ggagctgatc ttctcgcaaa accatctctt
     3841 gtgggcgcgc aatgtgccgc tcagccggct cgaattgcgt ttcgcgcggc tggtgccgcg
     3901 caaatcaacc cgcgttgtcc tgtgtgacga cgatgacggt ctcgccgaac gcgctgcggt
     3961 cattctcgcc gacgccggct acaccgatgt ttcccgtctt gatggcggcg tggcggcctg
     4021 ggagaaagcc ggctatgtga ctttttcagg cgtgcatgtt ccgagcaagg cgttcggcga
     4081 attcgtcgag cacgacagca aaacaccgag cgtaagtgcg gccgagctcg atgccatgat
     4141 gcgcagcggc accgatctga aagtgctcga cagccggccc ttcgacgaat attcgcgcat
     4201 ttcgattccg gccggcatca atgtgccggg ggccgagctg gtgctgaggg tccgcgatat
     4261 tgtccgctcc cctgacacga cgatcgtcgt caattgcgcc gggcgcaccc gcagcattat
     4321 cggcgcgcaa tccctgatca atgccggcgt gacgaacaag gtcatcgcgc tgcgcaacgg
     4381 caccatgggc tggcacctcg cgggactcac ttgcgcgacc ggaaaatccg accgcgcgcc
     4441 ggacgtatcg aatgcgggtc tcgcatgggc gcgggatgcg gcggaaaaag ttgcgcgcag
     4501 gttcggcgtc acgcgcgtcg atcgcgcagc cgtcgagcgg tggcggcaag acgcggcacg
     4561 cacaacctat gtcttcgacg tgcgcgaacc ggccgaatac aaggcaggcc actatcatga
     4621 cgcgatttca gcgcccggcg gccagctggt gcaggcaacc gacatctacg ccggtgtgct
     4681 cggcgcgcga attgtgctca gcgacgacaa agaagtacgc gcactgatga ccgcgtcgtg
     4741 gcttacgcaa atggggtggc aggaagttta cgtgttgccg gaagcgggca gcgaaacgga
     4801 agaccccgcg ccgacgatcc tcggctcaat tccttcagac gcagccgtcg agccttcgaa
     4861 gattcttgag atcgacgacg tttccgtgat cgacgtctcc accagccctc actaccgccg
     4921 cggccatatc cccggcgcat ggttcgccat ccgcagccgc ctcgacaagg cgttgccgaa
     4981 aatcccgctg cggcagggcg tcatcctcac ctcggaagac ggtgtaacgg ccgccctggc
     5041 gcttgcagag gcccgcgccc tcaccacgtc gcccgtgcat tggctcaagg gcggcaatgc
     5101 ggggtgggcc gcagccggct tcccgctctc cacggcggag aaaatggccg acgatccgat
     5161 cgatgcgtgg ctcaagccgt acgagcaagc gaacgatcac gaagccgcca tgaatgccta
     5221 cctgtcctgg gaagtcgatt tgctggaacg tatccaaagg gacgggacga cacacttctt
     5281 gcacggacgt taaacaagcc gtcgtggccg ggcaaccggg tccggccgaa ggccggcccg
     5341 gtcgtaaact tgtcaccgca agtcggatac atccgacttg cggcagt
//
LOCUS       DASSRL010000632         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_944074, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000632 DASSRL010000000
VERSION     DASSRL010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944074"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VFL49_12880"
     CDS             complement(<1..1215)
                     /locus_tag="VFL49_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7912920.1"
                     /translation="MPESIRTANERLRSRPWRDGFLATFIFASFSSLLSTPVRAETRL
                     GATLAGAVQRALATLREQEITALTLVLALLGVALLTTVVLFRLRRSADRAEFNARDEA
                     AALRAEVDRLKALLLSEPQVLVTWPVASEQPEIIGDTTMLAPGGTRERVLAFGTWLEG
                     AAARRMEQAVDTLRNDGRGFAMTVTSRGGRPVEVEGRVVAGRAILRLRDVSGIERELL
                     DLAARHDELLGDVEIMRSLLDTLSAPVWARDAAGRLIFVNTAYARAVEAENPTDAVAR
                     ELELLDQSTRTQVARARVAGQTYVGRLPAVTAGVRRVFDVIDASSGAGSAGMAIDRTE
                     AETLRAELMRMTEAHRRVLDQLSTGVAVFNIDRKLTFFNSAFRSLFELDAGFLDQGPT
                     DAAVLDFLRDRRK"
     gene            complement(1389..1844)
                     /gene="dut"
                     /locus_tag="VFL49_12885"
     CDS             complement(1389..1844)
                     /gene="dut"
                     /locus_tag="VFL49_12885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7912921.1"
                     /translation="MSGIDVRVVRLAHAKDLPLPAYQTEQAAGLDLLAAVPPATPVTL
                     SPGERGLIPTGLVIALPSGHEGQVRPRSGLAARYGLTIVNAPGTIDADYRGEIQVLLV
                     NLGGDPVTINRGMRIAQLVIAPIVRAHIVETESLDKTSRGAGGFGSTGR"
     gene            complement(1841..3265)
                     /gene="coaBC"
                     /locus_tag="VFL49_12890"
     CDS             complement(1841..3265)
                     /gene="coaBC"
                     /locus_tag="VFL49_12890"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7912922.1"
                     /translation="MASLTVRQLDENLKKRLRLRAAKNGRSVEDEVRTILREAAEQTA
                     DEAAALLRSTAAANPGTKTSRPPLPDERKKVLLIIGGGIAAYKSLELIRRLKERKIAV
                     RCIMTKAAEHFVTPLSASALVGERVFTDLFDPASEFDVGHIRLARQTDLIVVAPATAD
                     LMAKLAGGHADDLATAVVLATSAKILLAPAMNPRMWANRVTQRNLAQLTADGIALVGP
                     NEGEMAEMHEQGLGRMAEPLEIAAFVEKLLTTRIASSLAGRRVLVTSGPTHEPIDPVR
                     YIANRSSGKQGHAIAVAAAAAGAEVTLVSGPVQIPDPPGVEVVKVESAREMLDGVESA
                     LPADVAIFAAAVADWRVANAAHQKLKKQSGKLTPELPLVENPDILATIAKRKTRRPKL
                     VIGFAAETENIVANARAKLAKKGCDWILANDVSPQSGIMGGDRNTITLVSNSGTETWP
                     PQSKEEVAALLITRISRTLDGATA"
     gene            3411..3761
                     /locus_tag="VFL49_12895"
     CDS             3411..3761
                     /locus_tag="VFL49_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7912923.1"
                     /translation="MPNDRLSATFSALADPTRRAILARLALGETTVSELAKPFKMSGP
                     AVSKHLKVLEHAGLIARGRTAQWRPCRLEPAALRGVDDWLETYRRLWGERLDRLEDYL
                     KTLQAEGKKNGRKK"
     gene            3745..4245
                     /locus_tag="VFL49_12900"
     CDS             3745..4245
                     /locus_tag="VFL49_12900"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7912924.1"
                     /translation="MGEKNSIDLDTDPCSIIGSRLFDAPRELVFSAFTDPKHLAQWWG
                     PNGFTTTTYNFEFRSGGVWRFVMHGPDGRDYQNRITYEEIIRPERIVYRHGGGEGVEP
                     VQFSQTLTFDDLGGGRTRLTWHGRFPSAEERARVIKDYGADKGLFQTMSRLADHVATM
                     ATEKAT"
     gene            complement(4258..4710)
                     /locus_tag="VFL49_12905"
     CDS             complement(4258..4710)
                     /locus_tag="VFL49_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007699434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7912925.1"
                     /translation="MSNPGAKNFELPLAATHLVRDNCLCLHVHRAARALARRFDDVLR
                     PLDLTNGQFSLLMSLNRPEPPTLGSVASLLAMDRTTLTANLKPLERRRLLKISVDKED
                     RRSRRLALTPAGRALLTKAYPIWKETHAEIDRLVGNADGLRKALRALS"
BASE COUNT          939 a         1456 c         1488 g          937 t
ORIGIN      
        1 ttttctcctg tcgcgcagga aatcgagcac ggctgcatcg gtcgggccct gatcaagaaa
       61 gccagcgtca agttcaaaca aagagcggaa tgcggagttg aaaaaggtca gcttgcggtc
      121 gatgttgaac acggcgacgc cggtcgatag ctggtcaagc acccgtcggt gcgcttcggt
      181 catgcgcatg agctctgcgc gcagagtttc ggcctcggtg cggtcgatgg ccatgccggc
      241 gctgccggct ccgctcgaag cgtcgatcac gtcgaataca cggcgcacgc cggcggtaac
      301 agctggcagg cgcccgacgt aagtctgacc ggcgacacgc gcgcgagcga cctgcgttcg
      361 tgtgctctgg tcgagcagct caagttcgcg ggcaacggcg tcggtgggat tttccgcttc
      421 gaccgcgcgg gcataggccg tattcacgaa gatcagtcgc ccggccgcgt cgcgggccca
      481 gacgggagcc gataacgtat cgagaaggga tcgcatgatc tcgacgtcgc ccagcaactc
      541 gtcatgacga gccgcaaggt ccagcagttc ccgctcgatg ccgctgacgt cccgcaggcg
      601 caggatggcg cggccggcaa caacacgtcc ttcgacttcg acgggacggc cgccgcggct
      661 ggtcacggtc atcgcaaatc cacggccatc gttgcgcaga gtgtcgacgg cctgctccat
      721 gcggcgtgcc gcggcccctt ccagccaggt gccgaatgcg agaacgcgct cgcgcgtgcc
      781 gcccggtgcg agcattgtgg tgtcgccgat gatctccggc tgctccgacg cgacaggcca
      841 ggtcacaagg acctgcggct cggacagcaa cagtgccttg agccggtcaa cctcggcacg
      901 gagtgccgcc gcctcgtcgc gcgcgttgaa ctccgcacga tccgcgcttc tgcgcaggcg
      961 aaacaggaca acagttgtga gcaatgcaac cccgagcagg gcgaggacaa gcgtcaatgc
     1021 cgtgatctcc tgctcccgca acgtggcgag cgcgcgctgc accgcacctg caagcgttgc
     1081 accgagccgc gtctcggcac gaaccggcgt gctgagcaag ctcgaaaaac ttgcgaagat
     1141 gaaggtggcg aggaatccat ctcgccacgg acgagaacgc agccgctcgt tcgccgtccg
     1201 gattgactcc ggcattcagg cctgccccag agatgcggag ggcgtccgcc acaccgttcc
     1261 gcgaatcgtt tgactttacc cccaacgcga atcgcgccga aagagtccag accgtgaaca
     1321 aggttttgga agagcctgat gtcagggcct aacgcctgca tccagcccga agtattcacc
     1381 aactctgctc aacgtccggt cgagccaaat ccgccggccc cgcgcgacgt cttatccaaa
     1441 gattcggttt cgacgatatg agcgcgtaca attggtgcga tgaccagttg ggcgatccgc
     1501 atgccgcgat tgatggtgac cggatcgccg ccaagattga ccagcagcac ttggatctcg
     1561 ccgcggtaat cggcgtcgat tgtgccgggc gcgttgacaa tggtgaggcc gtaccgggca
     1621 gccagccccg agcggggccg cacctgacct tcatggcccg aaggaagggc aatcaccaga
     1681 ccagttggaa taaggccgcg ctcgccggga ctgagcgtta ccggagtcgc aggtgggacc
     1741 gctgccaaaa ggtcgagtcc tgcggcttgc tcggtctggt aggccggtag cggcaagtcc
     1801 ttggcatggg caagacgcac tacgcgaaca tcgatgccac tcacgccgtc gctccatcga
     1861 gtgtccgcga tattcgcgtg atgagcaggg ctgccacctc ctctttgctc tgcggtggcc
     1921 aggtctccgt tcctgagttg ctgacgagcg tgatcgtgtt ccggtcgcct cccatgatac
     1981 cggactgcgg cgagacatcg tttgcaagaa tccagtcgca gcctttcttg gcgagtttcg
     2041 ccctggcatt tgccacaata ttctccgtct cggctgcaaa gccgatgacc agtttcggcc
     2101 gtcgcgtctt tcttttggcg atggttgcaa gaatgtccgg attttcaacc agcggcagtt
     2161 cgggcgtcaa tttgccggat tgttttttga gtttctggtg ggccgcattc gctacacgcc
     2221 aatctgcgac cgctgcagca aaaatagcga cgtccgcggg gagcgcgctt tcgacaccat
     2281 caagcatttc ccgggcgctt tcgactttga ctacctcgac gccaggcgga tcgggaatct
     2341 gcacaggccc gctcaccagc gtaacttcgg cgccggcggc agcggcggcg acggcgatcg
     2401 catggccttg cttgcccgac gagcggttgg cgatatagcg gaccggatcg atcggttcat
     2461 gtgtaggccc ggacgtcacc agcacgcgcc ggcccgccaa tgagctcgca attcgtgtcg
     2521 tcagcagctt ttcgacgaac gctgcgattt ccaacggttc agccatacgg cccaggcctt
     2581 gctcgtgcat ttccgccatt tcaccttcgt tcggaccgac gagtgcgata ccgtcggctg
     2641 tcagttgcgc cagattgcgt tgcgtcaccc tgtttgccca catgcgtggg ttcattgcgg
     2701 gcgccagcag gatttttgcc gaggttgcca ggactacagc ggtggctaaa tcgtcggcat
     2761 ggccgccggc gagctttgcc atcagatcgg cagtcgcagg cgcgactaca atcaaatcgg
     2821 tttggcgggc caggcgaata tgtccgacat cgaattcgct ggccggatcg aacaggtcgg
     2881 tgaacacgcg ttcgccgaca agcgcgctcg ccgagagcgg cgtcacgaaa tgctccgcag
     2941 cttttgtcat gatgcaacgt acggcgatct tgcgttcctt gagccggcgg atgagttcga
     3001 gagacttgta ggcggcgatt ccgccaccga taatcagaag cacttttttt ctttcgtcgg
     3061 gtagcggtgg acggctggtt ttcgtacccg ggttcgcggc tgctgtggag cgcagcagcg
     3121 ccgctgcttc atcagcggtc tgctcggccg cctcgcgcag gatcgtccgc acctcgtctt
     3181 cgaccgagcg gccgtttttg gcggcgcgca ggcgcaaccg ctttttgagg ttttcgtcga
     3241 gctggcgaac cgtcagcgag gccataaaat cctctgatat gaaaagctta atcgatgttt
     3301 gattgcaatg caatcattgc aatcaaatgg cttgcgttct ggtcttgacg ctgcgggacc
     3361 gcttctatat ttaaccgata cgttatataa ctatttagtt agatataatc atgccgaacg
     3421 atcgcctcag tgccaccttt tccgcgcttg cagaccccac gcgccgcgcc atccttgcgc
     3481 gtctcgcgct gggcgaaacc acggtcagcg agcttgcaaa gccgttcaag atgagcgggc
     3541 ccgccgtatc caagcacctg aaggtactgg agcacgcggg cctgattgcg cgcggacgca
     3601 cggcgcaatg gcgcccctgt cggctcgaac cggctgcgct caggggcgtt gacgattggc
     3661 tcgaaaccta tcgccgcctg tggggggaac gcctcgatcg tctcgaagac tatctcaaaa
     3721 ctctgcaagc ggaggggaag aaaaatgggc gaaaaaaata gcatcgatct cgataccgat
     3781 ccgtgctcga tcatcggtag ccggctgttc gatgcaccgc gcgaactggt attttcggcc
     3841 tttaccgacc cgaagcacct ggcgcaatgg tggggcccaa acggttttac gacaacaacg
     3901 tacaacttcg aattccggtc aggcggagtg tggcgtttcg tgatgcatgg tccggacggc
     3961 cgtgattacc agaaccgcat cacgtacgaa gaaattattc gacccgaacg tatcgtctac
     4021 cgccatggcg gaggagaagg cgtcgagccg gtccagttca gccagacctt gacctttgac
     4081 gatctcggcg gggggcgcac gcggcttaca tggcacgggc gattcccgtc ggcggaagaa
     4141 cgcgcccggg tgatcaagga ttacggcgcc gacaaaggcc tttttcagac catgtcgcgg
     4201 ctcgccgacc atgtcgcgac gatggcaaca gagaaggcga cataagtggc cacgcggtta
     4261 cgacagcgca cgcagtgctt tacgaagacc gtccgcgttg ccgaccaagc gatcaatttc
     4321 ggcatgagtc tctttccaga tcggataggc cttggtcaac agcgcgcgtc cggccggcgt
     4381 caaagcgaga cgccggctgc ggcggtcttc cttgtcgacc gaaattttca ggagacggcg
     4441 ccgttcaagc ggcttgaggt tggcggtaag cgtcgtgcgg tccatggcga gaagggacgc
     4501 aacactgcca agtgtcggag gctccggccg gttgagcgac atcagcagcg aaaattgacc
     4561 gttggtcaga tcgagcggcc gcagcacatc gtcgaagcgc cgcgccaatg cgcgcgcagc
     4621 gcgatgcacg tgcagacaga ggcaattgtc gcgcaccaga tgagtggcgg cgagcggcag
     4681 ctcaaaattc ttcgcgccgg ggtttgacac gaggtcatta tgttgatatc aacgtatttg
     4741 tcaatgctcg ctgggactgc aataccgttg tccccggatt ttcgaaacgg ccgcaaaatc
     4801 ggccacaaat aaggaggatc
//
LOCUS       DASSRL010000633         7000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_593817, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000633 DASSRL010000000
VERSION     DASSRL010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593817"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFL49_12910"
     CDS             <1..222
                     /locus_tag="VFL49_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912926.1"
                     /translation="MERAVVEGALEAARKDNSRLQSEVAALRSTLRRGAPLDDGPVPP
                     VEAANDETPHKGKRMRDTDGPQAEAGGKG"
     gene            326..562
                     /locus_tag="VFL49_12915"
     CDS             326..562
                     /locus_tag="VFL49_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912927.1"
                     /translation="MRVLYVAVPLLALLGFAVWFAATAWQRLGGEPIPAYGYAAIIGG
                     VLFSLLIGGGLMALVFYSNRHGYDDLSGGDNDRR"
     gene            654..1409
                     /locus_tag="VFL49_12920"
     CDS             654..1409
                     /locus_tag="VFL49_12920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7912928.1"
                     /translation="MNKSLVQEQFGKTAASYLTSAPHALGKSLERLVALTSPQKDWHV
                     LDVATGGGHVAYAFAPHVARVWATDITQEMLDMVKAEAQRRALPNVRTAYAKAEAMPF
                     DDASLDLVTCRIAPHHFESIPDFLGEVRRVLKPDGLFALVDNVVPEGSVGDYINAFER
                     FRDPSHLRAWTMQEWRDALKGAGLSVGHEEQIYKTMEFKNWASRHDATMQALLRSMLA
                     EVTPAVKTALEPQGAGGNLTFRLCEGLFIAKRA"
     gene            1638..3071
                     /locus_tag="VFL49_12925"
     CDS             1638..3071
                     /locus_tag="VFL49_12925"
                     /inference="COORDINATES: protein motif:HMM:NF039892.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912929.1"
                     /translation="MSKFGGFFARKAGLYDKPAEPTPSAISPPNDNPLELDEELFTAL
                     GAQLGGENESLRNLLLDANAKIGELDNIKTAVGKLVDPVSKALRAFEAEKSEKISLQT
                     VLNNTRTAYGKLRNEVAELEKKASLSEKECQALRHELTTTQNLLRTLEATKAEIAIDI
                     AARRAQIVDLETRLAQETGESKALREENRRLDERFSTADKRVMALESELNGARQRLLM
                     LEDEKRAQQVSLDKISADSARLSRKLAETEASLTATQGRLRHVEGNVAELNTERTRLV
                     AALDEANERHGHELTTQRMRFDALQARASASEKLLGEAREHLLARADEVRTYDRRAGE
                     LALERDALQTRVADLEAERIQRESEFREVDQARATLMERSAALARAFTAKEAALTRAE
                     DTIATLNEQIAAMEAARGAEKQAADQRIEELSTSLRREQMQCSVVEGALETARKDFAR
                     LMREAVALQREQAGPGDPALLRPANAA"
     gene            complement(3213..4388)
                     /locus_tag="VFL49_12930"
     CDS             complement(3213..4388)
                     /locus_tag="VFL49_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7912930.1"
                     /translation="MFISFFQELKQAGVPVTLREYLTLLEAMEVDLASRRVDDFYYLS
                     RAALVKDERNIDKFDRVFGHVFKGLETIDGVIEADIPAEWLKKLAEKYLTEEEKKQIE
                     AMGGLDKLLETLRKRLAEQKKRHQGGSKWIGTAGTSPYGAYGYNPEGVRIGQDENRNF
                     RAVKVWDKREFKDLDDEVEIGTRNIKLALRRLRKFARTGAPEELDLDGTIKGTARKGY
                     LDILMRPERHNAVKVLLFFDIGGSMDWHIKATEELFSAARSEFKHLQYFYFHNCLYER
                     VWKENRRRFQETTPTWDVLHTYPHDYKVIFVGDASMSPYEIVAPGGSVEHFNDEAGAL
                     WLERVIRTYPACVWLNPVAEREWDFTQSISMVRQLIGGRMFPLTLEGLDRAMRELVR"
     gene            4496..5857
                     /locus_tag="VFL49_12935"
     CDS             4496..5857
                     /locus_tag="VFL49_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912931.1"
                     /translation="MPMRDRARWAFAILFSVALLSAPAIWNRFPLLQYDTGGYLARWY
                     EPYLVPSRAVVYGLILNAGAALSFWPVVLAQSALTVWVIALILRVHGLGGRPRLLAGI
                     VAALSIFSTLPWLTSILLTDIFAGLAVLALYLLLLRADELSRREQFGLIALIAVSVAT
                     HSATFAVLLMLLSGAALLSLVDQHRLPRGRLFSGALAIGLGAALVFAANFTVTKRLVW
                     TPGGFALSFGRMLQDGIVKKYLDEHCPRARLVLCAYKDELPRDADEWFWGSPLFDKLG
                     RFAGLDHEMEQIALASLAEYPLLQIKTAAVATARQLIDVRTGEGVVNSIWHTYAIIER
                     YRPALLPEMRAARQQRGEIDFTIINRVQYPVALACLLLLPLIGGFMLRRKDMNDIGEL
                     AAFCLLALLANAFICGTLSNPHDRYGARLIWLAVAVTALAVLRLYEARERLRSHGIVA
                     SPR"
     gene            6011..6478
                     /locus_tag="VFL49_12940"
     CDS             6011..6478
                     /locus_tag="VFL49_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7912932.1"
                     /translation="MAKKALTVNGKRVAVTYDDPQMPLLYALRDDLGLHGPRFGCGLG
                     QCGACTVHIDGAAVRSCITPVSAVDVTKKIVTLEGLGSPEKPHPVQKAFIDEQAAQCG
                     YCINGMIMESAAFLARNKKPTDAAIKNALANNLCRCGTHARIVRAVKRASARV"
     gene            6490..>7000
                     /locus_tag="VFL49_12945"
     CDS             6490..>7000
                     /locus_tag="VFL49_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7912933.1"
                     /translation="MNIQISRREALLGSGALVVSFSLSGSSTGARAQNTAKPLALTEV
                     DSFLAIDKTGKVTVYSGKVDLGTGITTALRQIVAEELDVPMKRIELIQGDTLLTPDQG
                     KTWASVTIQVGGMQLRQAAAAARQALLAEAAKKLGTDNLTVADGIVMAGGRKKISYAE
                     LIGDKRFSIT"
BASE COUNT         1398 a         2181 c         2091 g         1330 t
ORIGIN      
        1 atggagcggg ctgtcgtcga gggcgcgctg gaagccgccc gcaaagacaa ttcccgcctg
       61 cagagcgaag tcgccgcgct acggtcgacc ctgcgccgcg gtgcgccgct tgatgacggt
      121 ccggtaccgc ccgtcgaggc cgccaacgat gaaacaccgc acaaaggcaa gcgcatgagg
      181 gataccgacg ggccgcaggc tgaggccgga ggcaagggct gaaccaggcg aacacaagat
      241 ttcaggcgcg ggcaaggtcc cgcgcttttt attttgatgc gggcggcgaa ctgacaaacg
      301 cccggaccgc gcctatatga cggatatgcg tgtcctttac gtcgccgtgc cactgctcgc
      361 actgctgggc tttgcagtct ggttcgccgc caccgcctgg caacggttgg gcggagagcc
      421 gatcccagcc tatggatacg cggccattat cgggggcgta ctgttttcgc tgctgatcgg
      481 aggcggcctg atggcgctgg tcttttacag taatcgccac ggctatgatg acctgagcgg
      541 cggcgacaac gatcgacgct gacccgtcat tctgaatatt cataggtgac gacgtatcgc
      601 ctgcgcgtga gacaggctat gtatgccgcg cgccggaagc gatcggggaa gttatgaaca
      661 agagcctcgt ccaggaacaa ttcggcaaga ccgcagcaag ctatctgacc agcgcgccgc
      721 atgcgctcgg caagagcctc gagcggctgg ttgccctcac ctcaccgcaa aaagactggc
      781 atgtcctcga tgtcgccacc ggcggcggcc acgtcgccta cgcattcgcg ccccatgtcg
      841 cgcgcgtatg ggcgaccgac atcacccagg aaatgcttga tatggtgaag gccgaggctc
      901 agaggcgtgc cttgccgaac gtccggaccg cttatgcaaa ggcggaagcg atgccgttcg
      961 acgatgcgag ccttgatctg gtgacctgcc gcattgcgcc acaccatttt gagtccatcc
     1021 cggacttcct tggcgaagtt cgccgcgtgc ttaaaccgga tggcttgttt gcgctggtag
     1081 acaatgttgt gcccgaaggc agcgtcggcg actacatcaa cgcgttcgag cgtttccgcg
     1141 atccgagcca cctgcgcgca tggacgatgc aggaatggcg cgacgcattg aagggcgccg
     1201 gcttgtcggt cggacacgag gagcagattt acaagacaat ggaattcaag aactgggcca
     1261 gccgccacga tgcaacgatg caggcgctgc tgcgctctat gctggccgag gtgaccccgg
     1321 cggtgaaaac ggcgctggag ccgcagggcg cgggcggaaa ccttaccttc cgcctttgcg
     1381 aaggcctgtt catcgccaaa cgcgcctgaa cgaaacgcaa ctgcgcgatc ccgcaagtgc
     1441 gatcggcggc ggctgaaatt ccgcattttg ccggctaaac ggccgaaaag gtgcctcccg
     1501 ccaagtgatt gccaggtcgt tgtattttgc cattaacctt tcatttaccc acagaaattt
     1561 agattccgtt aggatcggtt aacaatgtga ttcggcgtcg cttcggccgg gcgaatcggc
     1621 gtttagggga tcacccaatg agcaaattcg ggggcttttt tgcccgcaaa gccggactgt
     1681 acgacaagcc ggccgagcca acgccgagcg ccatctctcc tccgaacgac aatccgctcg
     1741 aactcgacga agaactattc acggcccttg gcgcccagct tggcggcgag aatgaatcgc
     1801 tgcgcaatct gctgctcgac gccaacgcca aaatcggcga gctcgacaac atcaagaccg
     1861 ccgtcggaaa gcttgtcgat ccggtgagca aggcactgcg cgcctttgaa gccgaaaaat
     1921 ccgaaaagat cagtttgcag acggtgctta acaacacgcg caccgcttac ggcaagctgc
     1981 gcaatgaggt cgccgagctc gaaaaaaagg ccagcctttc ggaaaaagaa tgccaggcgc
     2041 ttcgccacga gcttacgacg acgcagaacc ttcttcgtac gcttgaagcc acgaaggcag
     2101 agatcgccat cgatattgcc gcgagacgcg cgcagatcgt tgatctcgaa acgaggcttg
     2161 cccaggagac gggtgaaagc aaggcgttgc gtgaagaaaa ccgcaggctt gatgagcgct
     2221 tttcgacggc agacaagcgc gtgatggcgc ttgagtccga actcaacgga gcgcgccagc
     2281 gcctgctgat gcttgaagac gaaaaacgtg cacaacaggt ttcactcgac aagatcagcg
     2341 ccgattcggc gcgcctgtcc cgcaagctcg ccgaaaccga agccagcctg acggcgaccc
     2401 aaggacgtct gcgccacgtg gaaggcaatg ttgccgaatt gaatactgaa cggacgcggc
     2461 ttgttgctgc gctggacgag gcgaacgagc gccacggaca cgaactcaca acccaacgca
     2521 tgcgcttcga cgcactgcag gcgcgcgctt ccgcgagcga aaaactgctt ggcgaggccc
     2581 gcgaacatct gctcgcgcgt gcagatgagg tcaggaccta cgatcgccgc gccggcgaac
     2641 ttgcactcga gcgcgatgca cttcagacgc gcgtggccga tctcgaggcc gagcgaatcc
     2701 agcgagaatc cgaattcagg gaagtcgatc aggcgcgcgc aacgctcatg gaacgcagcg
     2761 ccgcgcttgc ccgcgccttt acggcaaagg aggcagccct gacgcgcgcc gaggacacga
     2821 ttgccacgct caacgagcag atcgccgcta tggaggccgc ccgcggcgcg gaaaaacagg
     2881 ccgccgatca aaggatcgag gagctcagca catcgctgcg gcgcgagcag atgcaatgct
     2941 ctgtcgtcga gggcgcgctc gaaaccgccc gcaaggactt tgcgcggctc atgcgcgagg
     3001 cggtggctct ccagcgcgag caggcggggc cgggcgatcc tgccctgctg cgcccggcaa
     3061 acgcggccta gagcctgatc cattcgggtg aacccgactc gtcatggccg ggcttgtccc
     3121 ggccatctac gtcttgcgtc gctcagtgcc gataaagcgt ggatgcccgc gacaagcgcg
     3181 ggcatgacga gtccacttga aagcattcgg ccctaacgca caagttcgcg catcgcgcgg
     3241 tcgagacctt ccagcgttag cgggaacatt cgcccgccaa tgagctggcg gaccatgcta
     3301 atggattgcg taaagtccca ctccctctcc gccaccggat tgagccagac gcaagctgga
     3361 tacgtgcgta tcacacgctc gagccagaga gcacctgcct cgtcattgaa gtgctccacc
     3421 gatccccccg gcgcgacgat ctcgtaaggc gacatcgacg catcgccgac aaagatcacc
     3481 ttatagtcgt gcggataggt atgcagcacg tcccacgtcg gcgtggtctc ctggaagcgg
     3541 cgacgatttt ccttccacac ccgctcatac aggcaattgt ggaaatagaa atattgaagg
     3601 tgcttgaact cgctgcgggc cgccgagaac aattcctcgg tcgccttgat atgccagtcc
     3661 atcgatccgc cgatatcgaa gaacaggagc actttcacgg cattgtgccg ctcgggccgc
     3721 atcaggatgt cgagatagcc cttgcgcgcg gtccccttga ttgtgccgtc gagatcgagc
     3781 tcttccggtg cgccggtacg cgcgaatttc cgcaggcggc gcagcgccag tttaatgttg
     3841 cgcgtgccga tctcgacttc atcgtcgaga tccttgaact cacgcttgtc ccacactttg
     3901 accgcgcgga aattgcggtt ctcgtcctgc cctatgcgca cgccctcagg gttgtaacca
     3961 taggcgccat agggtgacgt gccggccgtg ccgatccact tcgaaccgcc ctggtggcgc
     4021 ttcttctgct cggcgagacg cttgcgcaac gtctccagca gtttgtcgag accgcccatg
     4081 gcctcgatct gcttcttttc ttcctcggtc agatatttct cggcgagttt tttcagccat
     4141 tcggccggaa tatccgcttc aatcacgccg tcaatcgtct ccagcccttt gaacacgtgg
     4201 ccgaacacgc gatcgaattt gtcgatattg cgctcatcct tcaccagcgc ggcgcgcgac
     4261 aggtagtaga agtcgtcgac gcggcgcgac gccagatcga cctccatggc ctcgagcagt
     4321 gtcaggtatt cgcgcaaggt caccggcacg ccggcctgtt tgagctcctg aaagaaactg
     4381 atgaacatcg gcgcgcctta tatatgggcg gtattagcga gcattgtgcc ctagtcacga
     4441 aaccgtcaag gcggtatcaa atcaccaaca aaaagaacgt tctgggagga ttcagatgcc
     4501 aatgcgcgac cgcgcccggt gggcgtttgc catccttttc tcggtggcac tgctgagcgc
     4561 gccggcaatc tggaaccgtt ttcccctctt gcaatatgat accggcggct atctcgcccg
     4621 ctggtacgag ccctacctcg tgccaagccg tgccgtcgtc tacgggctga tcctcaacgc
     4681 cggggccgcg ttgtcgttct ggcccgtggt gctcgcgcaa agcgcgctca ccgtatgggt
     4741 gatcgcactg attttgcgcg tgcatggcct cggcggacgg ccaagattgc tcgccggtat
     4801 cgtcgcagcg ttgtcgatct tctccacatt gccctggctc acctcgatat tgctcacgga
     4861 tattttcgcc gggctcgcgg tgctcgcgct ttatcttctc ctgcttcgcg ccgacgagct
     4921 cagccgacgc gagcaattcg gtctgatcgc gttgatcgct gtttcggttg caacgcatag
     4981 cgcgacattt gccgtgctgc tgatgctcct gtctggtgcc gctctgcttt cactggtcga
     5041 tcagcaccgg ctgccgcgag ggcgtctttt cagtggcgcg ctcgcgatcg gtctcggtgc
     5101 agccctggtg tttgccgcca atttcaccgt caccaagcgg ctggtctgga cgccgggcgg
     5161 ctttgcgctt tccttcggac gcatgttgca ggatggcatc gtcaagaaat acctcgacga
     5221 acactgcccc cgtgcgcgcc tcgttctctg cgcttataag gacgaattgc cgcgcgatgc
     5281 cgacgaatgg ttctggggca gcccgctgtt cgacaagctc ggccgcttcg ccggcctcga
     5341 ccatgagatg gagcagatcg cgttggcgag cctcgccgag tatccgctgc tgcagatcaa
     5401 aaccgccgcc gtggcgaccg ccaggcaatt gatcgacgtc cgcaccgggg aaggcgtggt
     5461 gaattcgatc tggcacacct acgcaatcat cgaacgttat cgtccggccc ttttacccga
     5521 gatgcgggca gcacgtcagc aacgcggcga aatcgacttc acgatcatca atcgcgtgca
     5581 atatcccgtg gcgcttgcat gcctgctgct gctgccgctg atcggcggct tcatgctcag
     5641 gcgaaaagat atgaacgata tcggcgagct cgccgcattc tgcctgcttg cactgctggc
     5701 gaatgctttc atctgcggca cgctgtcgaa cccgcatgac cgttacggcg cgcgtctgat
     5761 ctggctcgcg gtcgccgtga cggcgctcgc tgttttgcgc ctgtatgaag cacgcgaacg
     5821 gctgcgctcc cacggtattg tggcctcccc gcgataaaag accgccttgg gccctccccg
     5881 gatttggcct agacttgaac tcttacaggt aatttctgga ctacggcccg gctggccggt
     5941 gtgagttcgt tgagacgcgg atcgcaagtc cgcgtccaag agtccgccgc gagcggaggg
     6001 ggagacatca atggcaaaaa aagcacttac cgtgaatggc aaacgtgtgg ccgtcactta
     6061 cgacgacccg cagatgccgc tgctctacgc actacgcgac gatctcggtc tgcacgggcc
     6121 acgctttggg tgcggcctcg gccaatgtgg cgcctgcacg gtgcatattg acggcgcagc
     6181 cgtgcgttcg tgcatcacgc cggtttcggc ggttgatgtg accaaaaaaa tcgtcacgct
     6241 ggaaggtctt ggctcgccgg aaaagccgca ccccgtgcag aaagcattca tcgacgagca
     6301 ggccgcgcag tgcggctatt gcatcaacgg aatgatcatg gaatcggcgg cgtttctcgc
     6361 caggaacaag aagccgaccg atgctgccat caagaatgcg cttgccaaca acctttgccg
     6421 ctgcggcacg catgcccgca tcgtgcgcgc ggtcaagcgc gcgtcagcac gcgtgtaagg
     6481 gaggcgcaca tgaacatcca gatttcccgg cgcgaggccc tgctcggttc cggtgcactc
     6541 gtcgtcagct tctccctgtc cggctcatcg accggagcgc gtgcgcaaaa tacggccaag
     6601 ccactcgccc ttacagaggt tgattccttc cttgccatcg acaagaccgg caaggtcacc
     6661 gtctattccg gcaaagtcga tctcggcacc ggcatcacga cggcgttgcg ccagatcgtg
     6721 gcggaagaac tcgacgtgcc aatgaaacgt atcgagttga tacagggtga caccctgctc
     6781 acgcccgacc agggaaagac gtgggcgagc gttactatac aggttggcgg gatgcagttg
     6841 cgccaggccg cagccgctgc acgccaggca ctgcttgcgg aagccgccaa gaagctcggc
     6901 acggacaacc tcacggttgc cgacggcatc gtcatggccg gcggccgcaa aaagatcagc
     6961 tacgccgagc tgatcggcga caagcgcttc tcgatcacgc
//
LOCUS       DASSRL010000634         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_173880, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000634 DASSRL010000000
VERSION     DASSRL010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173880"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFL49_12950"
     CDS             <1..705
                     /locus_tag="VFL49_12950"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912934.1"
                     /translation="LLGPSGCGKSTLVQIIGGLEAATEGNVRIVDPARPDVARPLGAD
                     SVMVWQTLNLFPWRNVIDNVAFGLEMLGVSKAQRYERAKSLITSVGLAGFEQHLPGQL
                     SGGMRQRVALARALIMERPIMLMDEPFGALDAQTKIVMQEELLRVFDQTQKTIIFVTH
                     AIEEAILLADEIIVMTARPGRIKEVLHVDLPRPRSLEMVNSKAFGALFDRAFHLIREE
                     VMKSMAQQAMALEDSR"
     gene            702..1496
                     /locus_tag="VFL49_12955"
     CDS             702..1496
                     /locus_tag="VFL49_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7912935.1"
                     /translation="MKKRKSSLMRIAEAATPTAVILAFLLAWELFVRWRGIAPIYLPP
                     PSTILVYLFRMIGNGSIEYNLGVTLLRIFAGFAIAAFAGVTLGVLMGMSRLIARIVDP
                     WIAALYPLPKIALIPLLVIWLGTGELYKIVISAVTAFFPIVISTYAGIRQTDRGLIKA
                     AEDLGASRRQIQLKVVIPAAIPSIFAGLHLGMGVTVILIVAAEMIGSSSQSGMGFLLI
                     QSGQVMETEKVFASLVVLAIVGALIIKLQQWIDRKVAPWTAYRDGH"
     gene            1517..2572
                     /locus_tag="VFL49_12960"
     CDS             1517..2572
                     /locus_tag="VFL49_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912936.1"
                     /translation="MWSTSIRTAAAAGLIGWLAAAAVQPAAAADVVRVGEGPFITGGG
                     FYVANERGYFKKMGLDIQVKKFNDGALAVPAILAGELDITLMTANASFFNSVAKGAPL
                     VIVLDRGHNKPGYGYLAVNVTQELYDQGVRSLADFAKLKGKKMGVGAVGSINQYDTAR
                     ALQKAGLDPRKDVNWIVGVSQPDLMKMLGQKQVDLTDLAYQFGYFAQNNKWGPIVANG
                     DQIEPNSSIGVYAANKNFVKEKRDVLVRFAMAYLQGVKEFDAAATAPDSNQEILEILA
                     RNTFLNKPELIKAIAPYWSYTSEDGIPPIDSIMAMQDYWADYFTYVEKKATREQLFDL
                     SVAQEAKKRLDAEKPFN"
     gene            2695..>3316
                     /locus_tag="VFL49_12965"
     CDS             2695..>3316
                     /locus_tag="VFL49_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7912937.1"
                     /translation="MRNWLRLGACLIAAGLTLLAPGLAPAQEPIRIGVLLPLTGPFAK
                     NGIENWEAMQIARDMINERGGVNGRKVEFVNGDATSPAAAISETERLITKEGIKITTG
                     SFASPLAIAVSQAAERHGVFHWETTGAAEVITRRGFKHTFQVGAPARRYGQVAVDFVI
                     EDLSKQLKKPIADLRIALLWENRAFGKSVGDGIRAYTESKKIKLVYD"
BASE COUNT          688 a          935 c         1042 g          651 t
ORIGIN      
        1 ctgctgggac cgagcggatg cggcaagagc accctggtcc agatcatcgg cggacttgaa
       61 gcggccacgg aagggaatgt gcgaatcgtc gatccggcgc ggcccgatgt agcgcgcccg
      121 ctcggggccg actcggtgat ggtctggcag acgctcaatc tttttccctg gcgcaacgtc
      181 atcgacaatg tcgccttcgg cctggagatg ctgggcgtat cgaaggcgca gcgttacgag
      241 cgtgcgaagt cgttgattac ctcggtgggg cttgccggat tcgagcagca tcttccaggg
      301 caactctcgg gcggcatgcg ccagcgcgtg gcgctcgcgc gcgcgttgat catggagcgt
      361 ccgatcatgc tcatggacga gccattcggc gctctcgacg cgcagacgaa aatcgtgatg
      421 caggaagaac tcctgcgcgt cttcgaccag acgcagaaga cgatcatctt tgtcacgcac
      481 gcaattgagg aggcgatcct gcttgccgat gagatcatcg tgatgacggc caggcccggt
      541 cgcataaagg aggtgcttca tgtcgacctt ccgcgcccgc gctcgctcga aatggtcaac
      601 tcgaaggctt tcggcgcatt gttcgaccgc gcattccacc tgatccggga agaagtgatg
      661 aagtcgatgg cgcagcaggc catggcgctg gaggactcgc ggtgaaaaag cgaaaaagca
      721 gcctgatgcg aatcgccgaa gctgcgacgc cgaccgcggt cattcttgcc ttcctgctgg
      781 catgggagct gttcgtgcga tggcgcggga ttgcgccgat ctatctcccg ccgccgagca
      841 cgatccttgt ctatctgttt cgcatgatcg gaaacggatc gatcgagtac aatcttgggg
      901 tcacgttgct gcggatcttt gccggctttg cgatcgccgc atttgccggc gtcacgcttg
      961 gcgtgctgat gggcatgtcg cggttgattg cccgcatcgt ggatccgtgg atcgccgcac
     1021 tttatccttt gccgaagatc gcgctcattc ctttgctcgt aatttggctc ggcaccggcg
     1081 aactctacaa gatcgtgatc agcgcagtga ccgctttctt tccgatcgtg atcagcacct
     1141 atgccggcat ccggcaaacc gatcgcggat tgatcaaggc ggcggaggat cttggcgcta
     1201 gtcgacgtca gattcaactg aaggtggtta ttcccgccgc aatcccgagc attttcgcgg
     1261 ggcttcacct cggcatggga gttactgtta ttcttatcgt ggcggccgaa atgatcggca
     1321 gctccagcca gagcgggatg ggctttctgc tgatccagtc agggcaggtg atggaaacgg
     1381 aaaaagtctt tgcgagcctc gtcgtgctcg cgatcgtcgg tgcgctgatc ataaagttgc
     1441 agcaatggat cgaccggaaa gtggcgccat ggacagccta cagggacgga cactgaaatc
     1501 acgcagggag gcgaatatgt ggagcacttc aatacgaacg gcagccgcag ccggattgat
     1561 cggctggctg gccgctgcag ccgtgcaacc ggcggccgcc gcagatgtgg tccgcgtcgg
     1621 cgagggcccc tttatcaccg gcggcggatt ctacgtcgcc aacgagcgcg gctatttcaa
     1681 gaaaatgggc ctggatatcc aggtcaaaaa gttcaacgac ggcgcgctcg cggtgccggc
     1741 tattctggcg ggcgagctgg acatcacttt gatgacggca aatgcgagct tcttcaacag
     1801 cgtcgccaaa ggcgcgccgc tggtcatcgt tctcgaccgc ggccacaaca agccaggcta
     1861 cggctatctc gccgtgaacg tgacgcagga attgtacgac cagggcgtcc gttcgcttgc
     1921 cgatttcgcc aagctgaaag gcaagaagat gggagtcggc gcggtcggca gcatcaatca
     1981 gtacgacacg gcgcgcgcgc ttcagaaggc cggccttgat ccccgaaaag acgtgaactg
     2041 gattgtcggc gtgtcgcagc ccgatctgat gaagatgctc ggtcaaaagc aggtcgacct
     2101 gaccgacctc gcctatcagt tcggatattt cgcgcagaac aacaaatggg gaccaatcgt
     2161 cgcgaacggc gatcagatcg agccaaacag ctcgatcggc gtctatgccg ccaacaagaa
     2221 cttcgtgaag gaaaagcgcg acgtccttgt gcggtttgcg atggcctatc tgcagggcgt
     2281 caaggagttt gacgcggcgg cgaccgcgcc cgactccaac caggaaatcc tggagatact
     2341 ggcgaggaat acgtttctca acaagcccga gctgatcaag gccatcgcgc cgtactggtc
     2401 gtacacgagc gaggatggca ttccgccgat cgattccatc atggcgatgc aggattattg
     2461 ggcggattat ttcacttacg tcgaaaagaa agcgacgcgc gagcagctgt tcgatctttc
     2521 cgtggctcag gaggccaaga agcggctcga cgcggaaaag cctttcaact gacgggcgtg
     2581 tcagccgagg tctttgacag cgtcaattgt gtaggaacaa tgcgattgct gcatggcgcc
     2641 gccattgcta gcgttgacga ccgaaagaag aagttaaagg agggaggaac ggatatgcgt
     2701 aactggctgc gtctcggggc atgtctcatt gcagcgggtt tgacgttgct cgcgcccggt
     2761 cttgcgccgg cccaggaacc cattcgcatc ggcgtgctgc tgccgctcac cggtccgttc
     2821 gccaagaacg gcatcgagaa ttgggaagcg atgcagatcg cgcgcgacat gatcaacgag
     2881 cgcggcgggg tcaacggccg gaaggttgaa ttcgtgaacg gcgacgcgac cagtccggcc
     2941 gcggctatca gcgagaccga gcgcctgatc acgaaggagg gcatcaagat cacgaccggt
     3001 tcgttcgctt caccgcttgc tatcgccgtg agccaggctg ccgagcgtca tggcgtgttt
     3061 cattgggaaa ccacaggtgc ggcggaagtc atcacacgcc gcggcttcaa gcatacgttt
     3121 caggtaggcg ctccggcccg ccgttacggc caggtcgcgg tggacttcgt catagaggat
     3181 ctctcgaaac agctgaaaaa gcccatcgcc gatctacgca tcgcgctgct gtgggagaac
     3241 cgcgcattcg gcaaatcggt cggtgacggg attcgcgcct ataccgaaag caagaagatc
     3301 aagctggtct acgacg
//
LOCUS       DASSRL010000635         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_768958, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000635 DASSRL010000000
VERSION     DASSRL010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768958"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFL49_12970"
     CDS             <1..488
                     /locus_tag="VFL49_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HET7912938.1"
                     /translation="KDQAGKPIAGAEVDIWHSSPEGFYEQQDPKQAPMNLRGKFTTDL
                     QGHFSFRSVKPAGYPIPVDGPVGDLVRATGRHNYRPAHLHFLIFREGFKTLISQIYVN
                     DDEKLETDVQFGVTRALVGNYVRHGEAAPAADVKGEWYSLDQVFVMEPGKSRLPKPPI
                     R"
     gene            556..1164
                     /gene="pgsA"
                     /locus_tag="VFL49_12975"
     CDS             556..1164
                     /gene="pgsA"
                     /locus_tag="VFL49_12975"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7912939.1"
                     /translation="MNAISSRPLPARPLALPNLLTYARIAAVPVVVALIYWQSIFDGP
                     LWLRWVALAFFVAAGVTDILDGYLARTWGQQSSLGRMLDPIADKLLVASCLLMLAAEE
                     TIRGWTLLAAVVILCREILVSGLREYLAELRVGVPVTRLAKWKTTGQMVAVGFLIAGE
                     AGDRIVPVVTQIGIMLLWLSALLTLYTGWDYMRAGLRYMIDE"
     gene            1161..1418
                     /gene="moaD"
                     /locus_tag="VFL49_12980"
     CDS             1161..1418
                     /gene="moaD"
                     /locus_tag="VFL49_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HET7912940.1"
                     /translation="MNLKLRYFAWVRERVGKPEETVEIPPGIATVADLMTWLASRGEE
                     YAYAFENRNVIRAAINQSHVRPDTAIKGAGEIAFFPPMTGG"
     gene            1454..1933
                     /locus_tag="VFL49_12985"
     CDS             1454..1933
                     /locus_tag="VFL49_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET7912941.1"
                     /translation="MTPTVRLQREPFDAGAETAKLVRGRDDIGAVVTFTGICRGTENG
                     QPISALVLEHYPGMAEGEIERHVVEAAARWPLLGVTVIHRVGRIEPGEDIVLVVTTSS
                     RREAAFAAAEFLMDYLKTRAPFWKQVETAGGKAWVAAKAADDAAAERWTVRRRQAAE"
     gene            1954..2907
                     /locus_tag="VFL49_12990"
     CDS             1954..2907
                     /locus_tag="VFL49_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HET7912942.1"
                     /translation="MIPVVFAAVAALALAVNPASSNEVTYFAVPRGAHPHDVAPAPDG
                     NVWYTAQFQGALGILNPKSGEATQVSLGPDSSPHGVIVGPDRAAWITDGGQNAIVRFD
                     PATRAVKPFPLPKEFPGANLNTATFDRKGILWFTGQSGVYGRVDPSSGKVDAWKAPKG
                     TGPYGITTTPNGDVWYASLAGDHIAKIDTVSGDAFVVPPPKPGVGPRRIWSDSKGLLW
                     VSFWNTGEIGRYDPMNKTWKVWSLPKSKGGCYAIYVDNKDKVWVTDFVANAIIRFDPA
                     TEAFDSFPSDKRGASVRQLLGRPGEVWGAESGVDRLVVVKD"
BASE COUNT          538 a          851 c          956 g          600 t
ORIGIN      
        1 tcaaggacca ggccggaaag ccgattgcgg gtgccgaagt cgacatctgg cattcgtcgc
       61 ccgagggctt ctacgagcag caggatccca agcaggcgcc gatgaacctg cgcgggaagt
      121 tcacgaccga tctccagggc catttcagtt tccggagcgt gaagccggcc ggctatccga
      181 ttcctgtcga tggtccggtt ggcgacctcg tgcgtgcgac aggtcgtcac aactaccggc
      241 cggcgcattt gcactttctg atcttcaggg aagggttcaa gactctgatt tcgcaaattt
      301 acgtcaacga tgacgaaaag cttgagaccg acgtgcagtt cggcgtcacg cgcgcattgg
      361 tcggcaacta cgtgcgtcac ggcgaagccg cgccggcagc ggacgtgaaa ggcgaatggt
      421 attcccttga tcaggtattc gtgatggagc cgggcaagag caggctgccg aaacctccaa
      481 tccggtagaa cgaacggggg attgagtggc ggtgcccggt tgacctgacc gctttgcggg
      541 tgttcctttc cggccatgaa tgccatcagt tcccggccgt tgccggcgcg cccgctggcg
      601 ctgccgaacc tcctgaccta tgcgcgcatt gcggccgtgc cggtcgtcgt cgctctgatt
      661 tattggcaga gcattttcga cggacctttg tggttgcgct gggttgcttt ggcgtttttc
      721 gtcgcagccg gcgtcacgga catcctcgac gggtatctcg cacgcacgtg gggtcagcag
      781 tcttcgcttg gccgcatgct tgatcccatc gccgacaaat tgctggtcgc atcttgtctc
      841 ttgatgttgg cggcggagga gacaattcgc ggatggacat tgctcgcggc cgtcgtcatc
      901 ctctgccgcg agatcctggt gtccggcctg cgcgaatatc tcgccgagct gagggttggc
      961 gtgccggtga cgcgcctcgc caaatggaag acaaccgggc agatggtcgc cgtcggcttt
     1021 ttgatcgcgg gcgaagccgg cgatcggatc gtgcccgtcg tcacgcaaat cgggatcatg
     1081 ctgttgtggt tgtcggcgct gctgacgctc tataccgggt gggactatat gcgcgccggc
     1141 ttgcgttata tgattgacga atgaacttaa aattgcgcta tttcgcgtgg gtccgcgagc
     1201 gcgtcggcaa gccggaggaa accgttgaaa ttccgccggg catcgccacg gttgccgact
     1261 tgatgacctg gttggcttcg cgcggtgaag aatatgcgta cgcgtttgaa aaccggaatg
     1321 tgatccgcgc cgccatcaat cagagtcacg tgcggcccga cacggcaatc aaaggcgccg
     1381 gagaaatcgc gtttttcccg cctatgacgg gcggatagcg cgctcgccgt tggcgggtta
     1441 cattagtagt accatgacgc cgacagtgcg cctccagcgc gagccttttg acgccggtgc
     1501 ggaaaccgca aaactcgtgc gcggccgcga tgacatcggc gcggtcgtga cgttcaccgg
     1561 catttgccgt ggcactgaaa acggccagcc gatttcagcg cttgtgctcg agcactatcc
     1621 gggcatggcg gaaggcgaaa ttgagcggca cgtcgtcgag gcggcagcca gatggccctt
     1681 gctcggagtg acggttattc atcgggtcgg ccgcatcgag ccgggagaag acattgtgct
     1741 cgttgtgacg acgtcgtcac gtcgtgaggc agcttttgcg gccgccgaat tcctgatgga
     1801 ttatctgaag acccgcgcgc cgttctggaa gcaggttgaa acggcgggcg gaaaagcctg
     1861 ggttgctgcc aaagctgccg atgatgcggc ggcggagcgt tggacggtgc ggcgccgcca
     1921 agccgcggaa tagctggctg ctggaggggc actatgattc ccgtcgtgtt tgcggcagtt
     1981 gctgcgcttg cgctcgccgt caacccggca tcttccaatg aggtgacgta ttttgcggtt
     2041 cctcgcggag cgcatccgca cgatgtcgct ccggcgccgg acggcaatgt ctggtacacg
     2101 gcgcaatttc aaggcgcgct cggcattctc aatccgaaaa gcggtgaagc aacgcaggtt
     2161 tcactggggc cggattcgtc cccgcatggt gtcattgttg gacccgaccg cgcggcatgg
     2221 atcaccgatg gcgggcagaa cgccatcgtg agattcgatc ccgccaccag ggcggtgaag
     2281 ccttttccgc tgccgaaaga atttccaggc gccaacctca ataccgcgac tttcgatcgt
     2341 aaaggcattc tttggttcac cgggcagagc ggcgtctatg gccgtgtcga tccctcgagc
     2401 ggaaaagtcg acgcctggaa agcgccgaaa ggcaccgggc cctacggcat cacgacgacc
     2461 ccgaatggcg acgtctggta cgcctcgctc gcaggcgatc atatcgccaa gatcgatacc
     2521 gtgagcggcg acgcctttgt ggtgccgccg ccgaagcccg gcgtcggtcc gcgccgtatc
     2581 tggtccgatt caaaagggct gttatgggtg agcttctgga ataccggaga gatcggccgc
     2641 tacgatccga tgaacaagac ctggaaggtc tggagcctgc cgaaaagtaa gggcgggtgt
     2701 tacgcgatct atgtcgacaa caaagacaag gtttgggtca ccgacttcgt cgccaatgcg
     2761 atcatccgct ttgatccggc gaccgaagcc tttgacagct ttccgagcga caagcgcggc
     2821 gcttcggtcc gtcagttgct ggggcggccc ggcgaggtct ggggcgccga atcgggcgtc
     2881 gaccggctgg tcgtcgtgaa ggactgatgg caacgcgcaa cctgatgccg cccagtctgc
     2941 catcc
//
LOCUS       DASSRL010000636         6726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_296763, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000636 DASSRL010000000
VERSION     DASSRL010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296763"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..268
                     /locus_tag="VFL49_12995"
     CDS             29..268
                     /locus_tag="VFL49_12995"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HET7912943.1"
                     /translation="MRILTVAALFVLGFTTASIAANDGPWCYRDFGGPQYTNCPYHSI
                     RECLTAAGILGGVCERNHGPAPEAQKKDKRKAQSR"
     gene            complement(265..582)
                     /locus_tag="VFL49_13000"
     CDS             complement(265..582)
                     /locus_tag="VFL49_13000"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7912944.1"
                     /translation="MIVTVFRSRLRPGAREDYIALVDRMVELAKTMPGYISHKGFFAD
                     DGERVTIVEFEHKEGMRAWRMNPEHRAAQKKAKEIYYSEYSVQVCNVVRESKFRLDER
                     APA"
     gene            complement(683..1096)
                     /locus_tag="VFL49_13005"
     CDS             complement(683..1096)
                     /locus_tag="VFL49_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET7912945.1"
                     /translation="MKSQFDDWRGSAAPSLSDIEGLASEAYVRLPEHFRNLCQNLVIR
                     VEDFPTDEVLENMHAESEFDLLGLFQGVGLPFQSDSVPEVMPNMIWLYRRPILDYWAE
                     HEDTLGEIVTHVLVHEIGHHFGLSDEDMEAIEASV"
     gene            complement(1894..3297)
                     /gene="leuC"
                     /locus_tag="VFL49_13010"
     CDS             complement(1894..3297)
                     /gene="leuC"
                     /locus_tag="VFL49_13010"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET7912946.1"
                     /translation="MKPRTLYDKIWEDHLVDEQPDGTCLIYVDRHLVHEVTSPQAFEG
                     LRTAGRKVHAPDKTLAVVDHNVPTTDRSQPNPDPESAEQIAALAKNAKDFGIEYFNEF
                     DKRQGIVHIIGPEQGFTLPGTTIVCGDSHTATHGAFGALAYGIGTSEVEHVLATQTLI
                     QKKSKNMRAVVDGKLPPGVTAKDIILAIIGEIGTAGGTGYALEYAGEAIRATSIEGRM
                     TICNMSVEGGAKAGFIAPDEIAYTYLKDKPKAPKGRAWDEAMRYWETLRSDDGAHFDR
                     EIRLDAAKLPPLVTWGTSPEQVTSITGKVPVASEIVDEKKRIAAERSLEYMGLKGGEK
                     ITDLKLDRVFIGSCTNARIEDLRAVARVVEGKAVNANVNAMIVPGSGLVKMQAEAEGL
                     DKIFKAAGFEWREPGCSMCLAMNPDKLQPGERCASTSNRNFEGRQGYKGRTHLVSPAM
                     AAAAAIAGHFVDVREWT"
     gene            complement(3549..3947)
                     /gene="rplS"
                     /locus_tag="VFL49_13015"
     CDS             complement(3549..3947)
                     /gene="rplS"
                     /locus_tag="VFL49_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7912947.1"
                     /translation="MNIIQQLEKEQVEKLSAGKTIPDFQPGDTVLVNVKVVEGDKARV
                     QAYEGVCIGRSGRGINENFTVRKISYGEGVERVFPLYSPMIDSIQVVRRGKVRRAKLY
                     YLRGLRGKKARISEAQGAGAAEGAEAAANE"
     gene            complement(4173..4868)
                     /gene="trmD"
                     /locus_tag="VFL49_13020"
     CDS             complement(4173..4868)
                     /gene="trmD"
                     /locus_tag="VFL49_13020"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7912948.1"
                     /translation="MWRASVLTIFPEMFPGPLAQSLAGKALTSGIWSLDTVDIRAHAN
                     DKHRTVDDTPAGGGPGMVMKADVLAHALDSLPHDTRPRLLMSPRGKPLRQNRAAKLAH
                     GLGVVLVCARFEGVDERLIKGRGLEEVSLGDFILSGGEIAALALLDACVRLLPGVMGK
                     EASAAEESFADGLLEYPQYTRPPVFEGEPIPEVLTSGDHAKVKAWRRTEAERLTAERR
                     PDLWSAYQARKKP"
     gene            complement(4871..5389)
                     /gene="rimM"
                     /locus_tag="VFL49_13025"
     CDS             complement(4871..5389)
                     /gene="rimM"
                     /locus_tag="VFL49_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663539.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7912949.1"
                     /translation="MAERICVARIGAAHGVRGEVKLWSFTEDPASVAAYGPLETEDGM
                     QRFEIEAMRPAKDHFVARIAGVNDRDAAERLRNLELYIPRARLPRIDEQDTYYHADLI
                     GLDVVTPEGVQVGTVHALHNFGAGDIIEIMPVGSGEPLMLPFNDATVPKVDLEAKQIV
                     LVHPIEIEARDE"
     gene            complement(5408..5830)
                     /gene="rpsP"
                     /locus_tag="VFL49_13030"
     CDS             complement(5408..5830)
                     /gene="rpsP"
                     /locus_tag="VFL49_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7912950.1"
                     /translation="MSLVIRLARAGTKKRPVYHVVVADNRAPRDGRFIERLGYFNPLL
                     PKEKTERLKLDLEKVQAWMKKGAQPSDRVMRFLDTAGIMKRPKRNNPEKAIPRKERKA
                     KEEEAAKASGGAPAADGAKPAEKPADKPAEAAPAPAAS"
     gene            complement(5867..>6726)
                     /locus_tag="VFL49_13035"
     CDS             complement(5867..>6726)
                     /locus_tag="VFL49_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7912951.1"
                     /translation="LTGQDAVTTARAFDERLDVTGIVLTRVDGDGRGGAALSMRAVTG
                     KPIKLIGTGEKLDALEEFDPARIAGRILGMGDIVALVEKAAANIDAEKAMRAAEKMRK
                     GAFDLADLREQLTQMQAMGGLSGLMGMLPGIAKMKNQLAAANLDENVIKRQIAIIDSM
                     TPKERRSPDVLKASRKRRIAAGSGTTPEAINKLLKMHRGMADMMKMMGGAKRGPLAGL
                     AQAMGFGSGMPSPEQMAEMAKSVPGGQLPPAMPKLPPNMPGLPGLPGLGGKFPGLPGG
                     LPPNLGKKK"
BASE COUNT         1160 a         2087 c         2082 g         1397 t
ORIGIN      
        1 atagcggatc gagaagagga ggacagatat gcgtattttg acggtcgcag cgctttttgt
       61 tcttggcttc acaactgcgt caatcgcggc aaatgacggt ccctggtgtt atcgcgactt
      121 cggtgggccg caatacacca actgccctta tcactcgatc agggaatgcc tgacggcggc
      181 cggaatttta ggcggcgtgt gcgagcgcaa tcacggtccg gcccccgagg cacagaaaaa
      241 ggacaaacgt aaagcacagt cgcgctaggc cggcgcgcgc tcatcgagcc tgaatttgga
      301 ctcgcgcacc acgttgcaca cctggacgct gtattcggag tagtagattt ccttcgcctt
      361 cttctgcgcg gcccgatgct cggggttcat gcgccaggcg cgcatccctt ctttatgctc
      421 gaactcgaca atggtaaccc gctcgccatc gtcggcgaaa aagcccttat gcgaaatgta
      481 gccgggcatc gtcttcgcca attcgaccat gcggtcgaca agggcaatat agtcctcgcg
      541 tgcgcccgga cgcaggcggg aacggaacac ggtcacgatc atgacgaatc tccttgtcgc
      601 gtgatgctcg cgccaccggt tgcgcccggc caattccaat tttgaatgat cgtcatcgaa
      661 atttcagatc gatgccagtc cattagacgc ttgcttcgat tgcctccata tcctcgtccg
      721 acaatccgaa atggtggccg atctcgtgca caaggacgtg ggttacaatc tcgcccagcg
      781 tgtcctcatg ttcggcccaa tagtcgagga ttggccggcg atagagccag atcatgttgg
      841 gcatgacctc cggcaccgaa tccgactgga acggcaaccc gacaccctgg aacagcccca
      901 gcaggtcgaa ctcactctcg gcatgcatgt tttcgaggac ttcatcggtc ggaaagtcct
      961 caacgcggat aaccaggttc tggcacagat tgcggaagtg ttccggcagg cggacatagg
     1021 cttccgaggc caggccttcg atatcggaca aagacggcgc cgccgagccg cgccaatcgt
     1081 cgaactgact tttcatagcc gtgttttagt ggaggtccgg acgcggacca agcggttgac
     1141 ggctcggccc cggatgcgca acggttggcg accctgacac gagacgcaaa ttgccgatgc
     1201 cgaagtttgc aacgcgattg gccctgatcg gctttatttt tgttctgagc gccggcccac
     1261 agaggccggc gtgggcgggg ccgtctgctt gtaatagcga aacctcggcg aattgtccgg
     1321 ctgagcttgc ggcgcagaaa aagcgcccgc acgtcacaat ttatccgcgc cgcgtctacc
     1381 ctgggccgaa tgccaagcgg cagtgccgcg cgtggctcgc gcaggaattc cgtgtcagcg
     1441 gtccggtgat cgtgccgcaa atgcgttgtt ggtgggaata gagaacgccg cagcgctgag
     1501 atatattgct ttcgtgacgg ggtggcatcg gaggcgaagc gatgtctagg ttcacgaaaa
     1561 ccattgcgag tgcgaccttg attctaagca tccttgcgtt cgtgcctggc agcgcggaag
     1621 cgcgttggca tggaggctgg cgcggcgggt ggggttgggg acctgctgtc ggatttggcg
     1681 ttgggcttgg ctggggtaga ggatggggct ggggctatcc gtatgcctac tacccggggc
     1741 catattatta cgcggtgcca ccggcatgtg gctgggcacg cgtgcgcatc tggcgccacg
     1801 ggcattgggt gctccgccgc gcttggcgct gctggtaaga aaaagcccgc cggtaaagtt
     1861 ttggcgggct ctcttgatcg agctcttccc gggctacgtc cactcgcgca cgtcgacgaa
     1921 gtggccggcg attgccgcag ccgccgccat tgccggtgac acgagatgcg tgcggccctt
     1981 gtagccctgg cgcccttcga agttgcggtt cgacgtcgaa gcacagcgtt cgcccggctg
     2041 cagcttgtcc ggattcattg caaggcacat cgagcagccc ggctcgcgcc attcgaagcc
     2101 ggcagccttg aagatcttgt caagaccttc cgcttccgcc tgcattttaa cgaggccgga
     2161 cccgggaacg atcatcgcgt tgacattggc attgacggct ttgccttcca ccacgcgcgc
     2221 caccgcgcgc aggtcttcaa tgcgcgcatt ggtgcatgag ccgatgaaca cgcgatcgag
     2281 cttgaggtcg gtgatcttct cgccgccttt gagccccatg tattcgagcg accgttcggc
     2341 cgcgatgcgt ttcttttcgt cgacgatttc cgaggccacc ggcaccttgc cggtgatcga
     2401 cgtcacctgc tcggggctgg tgccccaggt gaccagcggc ggcaatttcg ccgcatcaag
     2461 acgaatctcg cggtcgaaat gcgcgccatc gtcggaccgc agggtttccc aatagcgcat
     2521 ggcctcgtcc caggccctgc ccttcggcgc tttcggcttg tccttgagat aggtataggc
     2581 gatctcatcc ggcgcgatga atccggcttt ggcgccgccc tccaccgaca tgttgcagat
     2641 cgtcatgcgg ccttcgatgg aggtcgctcg gattgcttcg ccggcatatt ccagcgcata
     2701 accggtgccg ccggctgtgc cgatctcgcc gatgatcgca agaatgatgt ccttggcggt
     2761 gacgccggga ggcaatttac catccaccac cgcgcgcatg tttttcgatt tcttctggat
     2821 cagcgtttgt gtcgccagca cgtgctcgac ctcggacgtt ccgatgccgt aggcgagcgc
     2881 gccgaaagcg ccgtgcgtcg cggtgtggct gtcgccgcac acgatcgtgg tgccgggcag
     2941 ggtgaagccc tgctcgggtc cgatgatgtg gacgatgccc tgacgcttgt cgaactcgtt
     3001 gaaatactcg atgccgaagt ccttggcgtt tttcgccagc gcagcgatct gctcggcgct
     3061 ttcaggatcg ggattgggct ggcttcggtc ggtggtcgga acgttatgat cgaccacggc
     3121 cagtgtcttg tcgggcgcgt gcaccttgcg gccggcggtg cgcaagcctt caaaggcctg
     3181 cggcgacgta acttcgtgca cgaggtgacg gtcaacatag atgaggcacg ttccgtccgg
     3241 ctgctcgtca accagatggt cttcccagat tttgtcgtag agcgtgcgtg gtttcatggc
     3301 gtcatcgttg ctcagaaaga attgtaaacg gctggcagtc gttcagcagg gtcaaaggct
     3361 tgcctgcacc gctgccgtca gccggccgaa gaaccgccac ggcaatcgcg cgtggtcggc
     3421 gagtacaatc cgctgagctt tggaaaccat gacggttata tagccaatca ttccgctgcc
     3481 gacaacgaat gagcgggcca agaaaaaggg ccgccgcagc ggccccttca ccccgaatag
     3541 ccgaaatatt attcgttcgc cgcagcctcg gcgccttccg ctgcgccggc accctgggct
     3601 tcggagatgc gcgctttctt gccgcgcagc ccgcgcaggt aatagagctt ggcgcggcgg
     3661 accttgccgc ggcggaccac ctggattgag tcgatcatcg gcgaatagag cgggaacacg
     3721 cgctcgacgc cctcaccgta agaaatcttg cgcacggtga agttctcgtt gatgccgcgt
     3781 cccgagcgac cgatgcagac gccttcgtaa gcctgcacgc gcgccttgtc gccttcgacg
     3841 accttgacgt taaccaacac tgtgtcaccc ggctggaagt cgggaatggt cttgcccgcc
     3901 gagagcttct cgacctgctc tttttccagc tgctggataa tgttcatcgc ggattctccg
     3961 tcggcagcgc cagcaaccgg cgcgcggtcc tgatctcgtc tgaatgggtt ggcgagcttg
     4021 tattacaaag ctttcggctt gtcacctctt cacaacctgt cagtcagtca catgtccggg
     4081 ccgggcccga agggtccgag acccggccat cccgctcagg caggcacttc tgcaatcgga
     4141 tcgccggaca agcccggcga tgactgatcc aattacggct tctttcgggc ctggtatgcc
     4201 gaccacaggt cgggccggcg ttcggcggtg agacgctcgg cttccgtccg ccgccaggcc
     4261 ttgaccttgg cgtggtcgcc ggaggtgagc acctccggga ttggctcgcc ttcgaaaacg
     4321 ggcggtcgcg tgtattgcgg atattccagc agcccatcgg caaaactttc ctcggcggcg
     4381 gaggcctcct tgcccatgac gccgggcagc aggcgcacgc aggcatccaa aagcgccagc
     4441 gcggcaatct ccccgccgga taaaatgaaa tcgccgagcg agacttcttc gagtccgcgc
     4501 cccttgatca gccgctcatc gacgccttcg aaccgtgcgc agacgaggac aacgcccagc
     4561 ccgtgcgcga gctttgccgc ccggttttgc cggagtggtt tgcctcgcgg actcatcagt
     4621 aagcgaggcc gcgtgtcgtg cggaaggctg tcgagcgcat gcgcaagcac atcggctttc
     4681 atcaccatgc caggaccgcc gccggcgggc gtgtcgtcga cggtgcgatg tttgtcattc
     4741 gcatgcgcgc gaatgtcgac ggtatcgagg gaccagatgc cggatgtcag cgcttttccg
     4801 gcaaggctct gcgccagcgg tcccgggaac atctccggaa agatggtcaa tacacttgcg
     4861 cgccacatgc tcattcgtcc cgcgcctcga tctcgatcgg gtgtacgagc acgatctgct
     4921 tcgcctccag atcgactttt ggcaccgtcg cgtcgttgaa cggcagcatc aaaggttcac
     4981 cgctgccgac cggcatgatc tcgatgatgt cgccggcgcc gaaattatgc agcgcgtgca
     5041 cggttccgac ctgaacaccc tcgggcgtaa cgacatcgag accgatcaga tcggcgtgat
     5101 agtaggtgtc ctgttcgtcg atcctgggca gccgtgcgcg cggaatgtaa agctcaagat
     5161 tacgcaatcg ttccgccgcg tcgcgatcat tgaccccggc aatacgcgcg acgaagtgat
     5221 cttttgccgg acgcatggcc tcgatctcga agcgctgcat gccatcttcg gtttccagcg
     5281 gaccgtaggc ggcgaccgac gcggggtcct ccgtgaacga ccagagtttc acctcgccgc
     5341 gcacgccgtg tgcggcaccg atgcgagcaa cgcaaattcg ctcggccacg gcgcgagggg
     5401 gcgagcctta gctcgccgca ggagcaggag cggcttcggc tggcttgtct gccggctttt
     5461 ccgcaggctt ggcaccgtcg gctgcaggcg cacctccgga agctttcgcc gcttcttctt
     5521 cctttgcctt gcgctccttg cgggggatcg ccttttccgg gttgttgcgc tttggccgct
     5581 tcatgatgcc ggcagtgtcg agaaagcgca tcacgcggtc cgacggctgc gcgcctttct
     5641 tcatccatgc ctggactttc tccaggtcga gcttcagccg ctcggtcttt tccttcggca
     5701 gcagcggatt gaaatagccg agccgctcga tgaagcgacc gtcgcgcggg gcacggttgt
     5761 cggcgacgac gacgtgatag accgggcgct tcttggtgcc ggcccgggcg agacgaataa
     5821 cgagagacat ccttttagtc ctttctcatt ctattgattg cgacgatcac tttttctttc
     5881 caaggttggg cggcaggccg cccggcaatc cgggaaactt tccgcccagt ccgggcaggc
     5941 caggtagtcc aggcatattc ggaggtagct tcggcatagc cggcggcagc tgaccgccgg
     6001 ggacgctctt cgccatctcc gccatctgct cgggcgacgg catgccggag ccgaaaccca
     6061 tcgcctgggc gaggccggcg agcgggccgc gtttggcgcc gcccatcatc ttcatcatgt
     6121 ccgccatgcc gcgatgcatc ttcagcagct tgttgatcgc ctccggcgtc gtgcccgagc
     6181 cggcggcaat gcgcctcttg cggctcgctt tgagcacgtc cgggctgcgg cgctccttcg
     6241 gggtcatcga gtcgatgatc gcgatctgac gcttgatgac attttcatcg agattggcgg
     6301 cggcgagctg gttcttcatc ttggcgatgc ccggcagcat gcccatcaat ccgctgagtc
     6361 cgcccatggc ctgcatctgc gtgagctgct cgcggaggtc ggcgagatcg aaggcgccct
     6421 tgcgcatctt ctcggccgcg cgcatggcct tttcggcgtc gatgttggcg gcagccttct
     6481 ccaccagtgc gacgatgtcg cccatgccga ggatgcggcc ggcgatgcgg gcgggatcga
     6541 attcttcaag cgcatccagc ttttcgccgg tgccgatcaa cttgatcggt ttccccgtga
     6601 ccgcgcgcat tgaaagcgca gcaccgccac gcccgtcgcc gtcgacacgg gtgagcacga
     6661 tgccggtgac atcgagccgc tcgtcgaagg cgcgcgcggt cgtcaccgcg tcctgaccgg
     6721 tgaggc
//
LOCUS       DASSRL010000637         5960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_296783, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000637 DASSRL010000000
VERSION     DASSRL010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296783"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="VFL49_13040"
     CDS             complement(<1..1752)
                     /locus_tag="VFL49_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7912952.1"
                     /translation="MRAADRSLDIIAFASEHFREIIRRRLSELAGLALLTLALLGALA
                     LATWSVQDPSLSHATQKPIHNLLGYPGAIFSDLAMQLLGLAAILLLVPEALLGWRLLS
                     HKTFGEKWRSLFWILTTFLGAAFASTLPRIGSWPLPTGLGGVAGDAMLRLPALIFGTP
                     LGGFTRLILCIVFGAATVTTLLIAAGGRTQARKTVTRDLEDEGLEEDEEDNGRDRGSA
                     LLGMLVHALLSWKARIGRMLRGEGRQRTPLPVVRGPRLEPRFDGDGHSDDYEDDYEEE
                     ESEPAPRRRAAPRATARRSGSGYMLPSLNLLAAPRASERTTLSREIIDGNAVALESVL
                     QDFGVRGEIINARPGPVVTLYELEPAPGIKSSRVIGLADDIARSMSAVSARVAVVSGR
                     NAIGIELPNPTREKVYLRELLTGLDYNETTAKLPLCLGKTIGGESVIVDLSRMPHLLI
                     AGTTGSGKSVAINTMILSLVYRLRPDQCRLIMVDPKMLELSVYDGIPHLLTPVVTDPK
                     KAVVALKWAVREMEGRYKKMSKLGVRNIEGYNARVAEAKAKGETLTRTVHTGYDKETG
                     EAIYEKEQLELEPLPFLV"
     gene            complement(1764..2981)
                     /locus_tag="VFL49_13045"
     CDS             complement(1764..2981)
                     /locus_tag="VFL49_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7912953.1"
                     /translation="MSMMTAKSEPVSFTAPAVDSRSPFVRLTELIAGIAPGKPVINLS
                     VGEPQHPVPSFVGPALAAHTADFGRYPANKGIEPFRHAVAAWLSSRYTLARPADPESE
                     VLVLNGTREGLFLAAIAAKRWVMRRAGKPAILVPNPFYAAYSAGALAADCEPVYLPTT
                     RATGFLPELGALDDALLARTVAFYIASPSNPQGAVADRKYLDRLVTLARRFGFLVFAD
                     ECYCEIYGKDAPPGMLEAAAPDFANVVVFHSLSKRSNLPGMRVGFAAGDRRFLASYLE
                     LRNVAAPQVPTPAQYAAIAAYQDEAHVKENRKLYSEKFDLADQIIGDRYGYERPAGGF
                     FLWLDVSKQGGSEIATKRLWAEAGVRVVPGRYLAREQNDGSNPGADYIRVAMVQEKTT
                     TAEALRRLVAALG"
     gene            complement(3245..4456)
                     /locus_tag="VFL49_13050"
     CDS             complement(3245..4456)
                     /locus_tag="VFL49_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7912954.1"
                     /translation="MLTSVALVLMMTIPGLGLFYGGMVRKKNVGDTVMTSFAVTCLVT
                     ILYAVITYSLAFTPGSAFIGGTTRAFLQGILSDISNGTGTPNGLAVTIPETVYLCFQM
                     TFAIITPALIAGAFAERMKFSAMLWFVGLWAILVYAPIAHWVWGSDGFMSNGNSDAMF
                     KVLDFAGGTVVHINAGVAGLMCAICLGKRKDMGPPHNMVLTFIGASMLWVGWFGFNAG
                     SAVTAGMQAGMAMTVTQIATAVAGFTWMLVEWICRGKPTVIGICSGAVAGLVAITPAS
                     GFVGPAGSMAIGVAAGVLCYWGVTGLKRILGADDALDCFGVHGVGGIVGALLTGVFAI
                     SEYGGTAGLIEGNAAQMINQFQGVAVVIIYDAIVSLVLLKIIDAVIGLRVSEEVEREG
                     LDLALHGEVVQ"
     gene            complement(4722..5060)
                     /locus_tag="VFL49_13055"
     CDS             complement(4722..5060)
                     /locus_tag="VFL49_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7912955.1"
                     /translation="MKIVMAIIKPFKLDEVRDALTSIGVQGLTVSEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKIKIEVAVDTKQVDKVIGAITGAAKTGQIGDGKIFVFNLENAVRI
                     RTGETDAAAL"
     gene            complement(5370..>5960)
                     /locus_tag="VFL49_13060"
     CDS             complement(5370..>5960)
                     /locus_tag="VFL49_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HET7912956.1"
                     /translation="FNGGSALGANSRAAMAIVSTHLAASAGAFTWMALEWWTRGKPSV
                     LGMISGAVAGLGTITPASGFVLPMQGVLIGIIAGAICFWACTWLKLKVGYDDSLDVFG
                     VHGIGGFTGTLLAGVFAVGALSATPETPGGIPGLLEGNPHQVVAQLYGIIVTLVWSGA
                     VTFVILKVISVLVPLRVKQEDEVMGLDVSQHGEAIQ"
BASE COUNT         1173 a         1844 c         1895 g         1048 t
ORIGIN      
        1 cacgaggaac ggcagcggct caagctcgag ctgctccttt tcgtagatcg cctcgccggt
       61 ttccttgtcg taaccggtgt gaaccgtgcg tgtgagcgtc tctcctttgg ccttcgcctc
      121 agcgacgcgc gcattgtaac cctcgatgtt gcgcaccccg agcttcgaca ttttcttgta
      181 gcggccttcc atctcgcgca ccgcccattt gagcgcgaca accgcttttt tcggatcagt
      241 gacgaccggt gtaagaagat gcggaatgcc gtcatagacc gagagctcga gcatcttggg
      301 atccaccatg atcaggcgac attgatcggg ccgtagccga tagaccagcg acaggatcat
      361 ggtgttgatg gcgaccgact tgcccgatcc ggtcgtgccg gcgatcagca gatgcggcat
      421 gcgcgagaga tcgacaatga cggattcgcc gccgatggtc ttgccgaggc agagtggcaa
      481 tttcgcggtg gtctcgttat agtcgaggcc ggtgagcagc tcgcgcaggt agaccttctc
      541 gcgtgtcggg ttcggaagct cgattccgat ggcgttgcgt ccagacacaa ccgcgacgcg
      601 ggccgagacc gcgctcatcg agcgggcgat gtcgtcggca agtccgatca cgcgcgaaga
      661 cttgatgccg ggtgccggct caagttcgta gagcgtgacc accgggccgg ggcgcgcatt
      721 gatgatttca ccgcgaacgc cgaaatcctg cagcacgctt tcgagggcaa ccgcgtttcc
      781 gtctatgatt tcgcgcgaca atgtcgtgcg ctcgctggcg cgcggcgcgg ccagaagatt
      841 gagcgagggc agcatgtagc cgctgcccga acgtcgcgcg gtcgcgcgcg gtgccgcgcg
      901 cctgcgaggc gccggctcgg attcctcttc ctcgtagtcg tcctcgtagt cgtcgctatg
      961 tccgtcacca tcgaaccgag gttcgagccg cggcccgcgc acgaccggca acggcgtacg
     1021 ctggcgtccc tcgccgcgca gcatccggcc gatgcgcgcc ttccagctca acagcgcatg
     1081 caccagcatg ccgagcagtg cggaaccgcg gtctcgaccg ttgtcctcct cgtcctcctc
     1141 aagcccttcg tcctcgagat cgcgggtgac ggtctttctg gcctgggtgc ggccgccggc
     1201 ggcgatcaat aacgtcgtca ccgtcgccgc accgaacaca atgcagagga tcaaccgggt
     1261 aaagccaccg agcggtgtgc caaagatcag ggcgggcagc cgcaacatcg cgtcgccggc
     1321 gacgccgccg agcccggtcg gcagcggcca agacccaatg cgcggcagcg tggaagcaaa
     1381 tgccgcccca aggaacgtgg tgaggatcca gaacaggctg cgccatttct cgccgaaggt
     1441 cttgtgcgac agcaaccgcc atccgagcag cgcctcgggc acgagaagca ggatcgccgc
     1501 gagaccgagc agctgcatcg ccagatcaga aaaaatcgcg cccggatagc ccagcagatt
     1561 gtggatcggc ttctgcgtcg cgtggctgag cgaggggtcc tgcacagacc aggtggcgag
     1621 cgccagggcg ccgagcaatg ccagggtgag tagcgcgagg ccggcgagct cgctcagccg
     1681 gcgccggatg atctcgcgga aatgctccga agcgaaggcg atgatgtcga ggctgcgatc
     1741 tgctgcccgc atgcgttaca tccttatccg agcgcggcca cgaggcgccg cagcgcctct
     1801 gccgtggtcg tcttttcctg caccatagcg acacggatat agtccgcgcc gggattgctg
     1861 ccgtcgtttt gctcgcgcgc gaggtaacga cccggaacga cccgaacgcc ggcttcagcc
     1921 cataaccttt ttgtcgcgat ctcgctgccg ccctgtttgg acacatcgag ccacaggaag
     1981 aaaccgccag ccggacgctc gtagccgtag cggtcgccga taatctgatc cgcgagatcg
     2041 aatttttccg aataaagttt tcggttttct ttcacatgcg cctcatcttg ataggcggcg
     2101 atcgctgcat attgcgcggg tgtcggcacc tgcggtgctg cgacgttgcg cagctcgaga
     2161 taggaagcga gaaatcgcct atcgccggcg gcaaatccga cgcgcatgcc gggcaggttc
     2221 gagcgcttgg acagcgagtg aaacaccaca acattcgcga aatcgggagc cgcagcttcg
     2281 agcatgccgg gcggtgcatc tttgccgtag atttcgcagt agcattcgtc cgcaaaaaca
     2341 agaaagccga agcggcgcgc aagtgttacc agcctgtcca gatacttgcg atcggcgacc
     2401 gcgccctgcg ggttcgaggg tgacgcgatg taaaaggcga cggtgcgtgc cagcagtgcg
     2461 tcgtcgagcg cgccgagctc gggcaggaag ccggtcgcac gagtcgtcgg cagatagacc
     2521 ggctcgcaat cggcggcgag cgccccggcg gaataggcgg cgtaaaacgg attgggaacg
     2581 aggatggcag gtttaccggc gcggcgcatc acccagcgct tggcggcaat ggccgcgaga
     2641 aacagtccct cacgcgtgcc gttgaggacc aggacttcgc tttccggatc ggcggggcgg
     2701 gcgagcgtgt agcggctact cagccatgca gcgactgcgt gccggaacgg ctcgatacct
     2761 ttgttggccg gatagcggcc gaagtcggcg gtatgcgcgg cgagcgccgg gccgacaaaa
     2821 gacggcaccg gatgctgcgg ctcccccacc gacaggttga tgacaggctt gcccggcgcg
     2881 atacccgcga tcagctcggt cagccgcacg aatggggagc gggaatcgac cgccggagcg
     2941 gtaaaggaaa cgggctcgct cttggcggtc atcatggaca tgcggttaca gggcgggtgc
     3001 cggcccgcca taaaaggctt ccacacaata aagacggcca ggttaagacc cgattaacca
     3061 tcggaaattc cggcgatttt tgaccgggtg gggcctgaaa acgagaccag aagagaggcc
     3121 ccgatccttg cgaaccgggg cctcgacgga ggggacggtg ccgggttccg tcccgcccgc
     3181 tagccttact tcactggcgc gatctttttc cgaaaaccgg gaccccgctt ttcgggatca
     3241 cgcgctactg aaccacctcg ccgtgcaaag cgagatcgag accttcgcgc tccacctctt
     3301 cggagacgcg caggccaatc acggcatcga tgatcttgag caggaccagc gagacgatcg
     3361 cgtcgtagat gataacgaca gcgacaccct ggaactggtt gatcatctgg gcagcgttgc
     3421 cctcgatcag tcctgccgtg ccgccgtact cggagatcgc gaacacgccg gtgagcagcg
     3481 cgccgacgat tccgccgacg ccgtgcacgc cgaagcagtc gagcgcgtcg tcggcaccga
     3541 ggatgcgttt cagtccggtc acaccccaat agcagagcac gccggcggcg acgccgatcg
     3601 ccatcgagcc ggcaggaccg acgaatcccg aagccggagt gatggcgacg agaccggcga
     3661 ctgcacccga gcagatgccg atgacggtcg gtttgccgcg acaaatccat tcgacgagca
     3721 tccaggtgaa acccgcgacc gcagtagcga tctgcgtcac ggtcatcgcc atgcccgctt
     3781 gcatgcccgc agtgacggcg gaaccggcgt tgaagccgaa ccagccgacc cagagcatcg
     3841 aggccccgat gaaagtgagc accatgttgt gcggcgggcc catgtccttg cgcttgccga
     3901 ggcagatcgc gcacatcagg ccggcgacgc ccgcattgat atgcacgacg gttccgccgg
     3961 cgaagtcgag gaccttgaac attgcgtcgc tgttgccgtt ggacatgaag ccgtcggagc
     4021 cccagaccca atgcgcgatc ggtgcgtaga cgaggattgc ccacaggccg acaaaccaca
     4081 gcatcgcgga gaacttcatc cgctcggcaa aagccccggc aatcagcgcc ggggtgatga
     4141 tggcgaaggt catttggaag cagaggtaga cagtctccgg aatcgtcact gccaggccgt
     4201 ttggcgtgcc ggtgccgttc gagatgtcac tcagaattcc ctgcaggaac gcgcgcgttg
     4261 ttccgccgat gaacgctgag ccgggcgtga aggcaaggct gtaggtgatg accgcgtaga
     4321 gaattgtgac aagacacgtc accgcgaaac ttgtcattac ggtgtcgcca acattcttct
     4381 tgcgcaccat gccgccgtag aagagaccga gccccggtat cgtcatcata agtacgagcg
     4441 caaccgaagt gagcatccac gcggtgtcgc cagaattcgg cgtgcacttc tcgagcacgg
     4501 ctttcttcgg atcggggtcg ttggcgcaag ctggaggcgg cgccaccggt gcggcgggtg
     4561 cagccgccgg cgcctgcgtc tgagcgtatg agacgtcata ggacggcgcg ctcaagaaaa
     4621 ggccgagcag aatccctaaa gcgagagcga agagtcccgc acgggaaaat ttcatcaacg
     4681 tcatggtcta aactcctgtc cgtttgtttg cggtgcgggt cctagagggc cgcggcatcg
     4741 gtctcgccgg tgcggatgcg cacggcgttt tccagattga agacgaagat ttttccgtcg
     4801 ccgatttgtc cggtcttcgc ggcacctgtg atggcgccga tgaccttgtc gacctgcttg
     4861 gtgtccaccg ccacttcgat cttgatcttc ggcaggaagc tcaccgcata ctcagccccg
     4921 cgatagattt ccgtgtgccc cttctgacgc ccgtatccct tgacctcgga aacggtcagc
     4981 ccttgcacgc cgatggaggt cagagcgtca cggacctcgt cgagcttgaa gggtttgata
     5041 atggccatga cgattttcat ggatttgatc cccgcttgag cccgtttgtt tcgttacgca
     5101 gtgcgggcgg gttctctggg cctccgcccg cgaagcttgc ttcgcaggca tggccgcagg
     5161 gatagaatca aacgccgtgc cagatggccg gcctgccttt aggccattga tcgcacttgt
     5221 aaaaaagtgc gtgaattgtt tgcggaggac agaggctcaa cgcggccgct caaagtcgcg
     5281 gcagggccgg aggtgcctgc ttaaaaatta gacagaaaaa tggccggttc cgcgccggtt
     5341 ccagtgtccg gcgcgggagc aaacgatcgc tactggattg cctcgccatg ctggctgaca
     5401 tcgaggccca tgacctcgtc ttcctgcttc acgcgcagcg gcacgagtac actgattacc
     5461 ttcaggatca cgaaggtcac ggcacccgac caaaccagcg tgacgataat tccgtagagc
     5521 tgggcgacca cctgatgtgg attgccttcc agtaagcccg gaattccgcc gggagtttca
     5581 ggagttgcag acagcgcccc gaccgcgaac acgccggcga gcagcgtgcc ggtgaagccg
     5641 ccgatgccgt gcacgccgaa cacgtcgagc gagtcatcgt atccgacctt aagcttgagc
     5701 caggtgcagg cccagaagca gatggcgccg gcaatgatgc cgatcagaac gccttgcatg
     5761 ggcagcacga aaccggatgc cggagtgatc gtgccgagac cggcgacggc ccccgagatc
     5821 attccgagca ccgacggctt gccccgcgtc caccattcaa gcgccatcca tgtgaaggca
     5881 ccggcacttg cggcaagatg cgtcgatacg atcgccatgg cagcgcgtga attggcgcca
     5941 agcgcggagc caccgttgaa
//
LOCUS       DASSRL010000638         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_331260, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000638 DASSRL010000000
VERSION     DASSRL010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331260"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFL49_13065"
     CDS             complement(<1..728)
                     /locus_tag="VFL49_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET7912957.1"
                     /translation="MSLPMVEGEFKDTGRPRAQVALTIKDFKGKAEPDWCPGCGDFGV
                     LSALKQALAELSIRPHEAMVISGIGCSSNLPGYISTYGMHTLHGRALSVATGAQLANH
                     EIKIIVTGGDGDGYGIGGNHFIHSMRRNVDLTYIVMNNQIYGLTTGQISPTSTKGMAT
                     KSTPFGSVENPLNPIPLAIAAGATYVARGYTGQVKHLVELIKGGIEHKGFALIDAFSP
                     CVTFNHDNSHEFFKQRTFKLEDRK"
     gene            complement(814..>2032)
                     /locus_tag="VFL49_13070"
     CDS             complement(814..>2032)
                     /locus_tag="VFL49_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7912958.1"
                     /translation="DTGKRLAFSEGNQALAMGGAAAGVRFYCAYPMSPSSGVLHWMAR
                     HARQLGIMVRQVEDEIGVINMAIGAAHAGCRAMCATSGGGFALMTEGLGAAAMMEIPV
                     VCINVQRAGPATGVPTKTEQGDLWQVMGAGQGDYPRIIVAPTNIADCFKTVPQLFNLV
                     DKFQCPGIIISDLLLSEGRSSIDPAELDFNVPIDRGAVIGLNGAPPLNGGYKRYQLTD
                     SGISARALPGTPGFEHVVATDDHDEEGVLISDEYTNPHKRQAMHEKRMRKMESVLSLL
                     EAPKPWGAADAQVTLLGWGSTEGVVREAIQKLADEDGIAANQLQIKWIVPLHSAAIAS
                     ALSKSRKVIIVENNYSGQFARYLRSETGIDADGHIRKYDGEPFMPHHIVDGVKAILAG
                     STKRYVPVHEIMV"
BASE COUNT          361 a          630 c          599 g          442 t
ORIGIN      
        1 tgttttctgt cttccagctt gaacgtccgt tgcttgaaga actcgtggct gttgtcgtga
       61 ttgaaggtca cgcagggact gaaggcatcg atgagcgcga aacctttgtg ctcgatgcca
      121 cccttgatca attcgaccag atgcttcact tgcccggtgt aaccgcgcgc cacgtaggtc
      181 gcgccggccg cgatggcgag cggaatcgga ttgagcggat tttccacgct accgaacggc
      241 gtgctcttcg tcgccatgcc tttggtgctg gtcggcgata tttgtccggt ggtgaggccg
      301 tagatctggt tgttcatgac gatgtaagtc agatcgacgt tgcggcgcat gctgtgaatg
      361 aaatgattgc cgccgatgcc gtagccgtcg ccgtcgccgc ccgtgacgat aatcttgatc
      421 tcatgattgg ccagctgagc gcccgtcgcc acggacagag cgcggccatg cagtgtgtgc
      481 atgccgtagg tgctgatgta acccggcagg tttgatgagc agccgatgcc gctgatcacc
      541 atcgcctcgt gcggccgaat gcttaactcc gcaagcgcct gcttcaatgc gctgagcacg
      601 ccgaagtcgc cgcagccggg acaccaatcg ggctcggcct tgcctttgaa gtctttgatc
      661 gtgagcgcta cctgcgcgcg cggcctgccg gtatctttaa attcgccttc gaccataggt
      721 aaactcatca ctcactcctt ttatcaactg cgagtctcgc gtcccgagtc tcgagtttcg
      781 ggttaaaccc aaaacccgta acctgaaacc tttctagacc atgatctcgt gcacgggcac
      841 gtatcgcttt gtcgatccgg ctaaaatcgc cttgacgcca tcgacgatat ggtgcggcat
      901 gaacggctcg ccgtcgtact tgcggatgtg accgtcggcg tcgatgccgg tttcggatcg
      961 tagatagcgg gcgaactggc cgctgtaatt gttttcgaca ataataactt tccggctctt
     1021 cgacagcgct gatgcgatcg ccgccgagtg cagcggaacg atccatttga tctgcagttg
     1081 attggcggcg atgccgtcct cgtccgccag cttttggatc gcctcgcgca ccacgccctc
     1141 cgtggagccc cagccgagca aggtaacctg agcgtctgcg gcgccccaag gcttcggcgc
     1201 ttcgagcagg gataagaccg attccatctt gcgcatgcgc ttctcatgca tggcctggcg
     1261 tttgtgcggg ttggtgtatt catcgctgat caagacaccc tcctcgtcgt gatcgtcggt
     1321 ggcaacgacg tgctcgaagc ctggcgttcc gggcagcgcg cgcgctgaga tgccgctgtc
     1381 ggtgagctgg tagcgtttgt agccgccgtt aagcggcgga gcaccgttca gaccgatcac
     1441 cgcgccgcgg tcgatgggca cattgaaatc caactccgcg gggtcgatgc tcgatctgcc
     1501 ttcagagagc aacaaatcgg agatgataat gccggggcat tgaaacttgt cgaccaggtt
     1561 gaatagctgc ggcacggtct tgaagcaatc cgcaatattc gtcggcgcga cgatgatgcg
     1621 cggataatct ccctgccccg cgcccatcac ttgccagaga tcgccctgtt cggtttttgt
     1681 cggcacgccc gtcgccggac cggcgcgctg cacgttgata caaaccaccg ggatctccat
     1741 catcgcagcc gcgccgagcc cctcggtcat caacgcaaag cctcctcccg acgtcgcgca
     1801 catcgcgcgg cagccggcgt gagcagcgcc gatcgccatg ttgatgacgc cgatctcgtc
     1861 ttccacctgg cgcaccatga ttcccagttg ccgggcgtgg cgcgccatcc agtggagcac
     1921 gccggacgac gggctcatcg ggtaggcgca atagaaccgc acgcccgccg ccgcgccgcc
     1981 catcgccagc gcctggttgc cttcggagaa cgcgagcctc ttgccggtgt cc
//
LOCUS       DASSRL010000639         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1696737, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000639 DASSRL010000000
VERSION     DASSRL010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696737"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFL49_13075"
     CDS             <1..201
                     /locus_tag="VFL49_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7912959.1"
                     /translation="DKPGFIGKFSSTLGEAGINIATFHVGRDAPGGNAVALIEIDGKL
                     PEVVLAKVRALPQVQQAKPLHF"
     gene            360..1655
                     /locus_tag="VFL49_13080"
     CDS             360..1655
                     /locus_tag="VFL49_13080"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7912960.1"
                     /translation="MANVVVVGSQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLV
                     IDGSTYKLSLLPSGVVREGKLSVIGNGVVIDPQALIDEIAQLKKQGIVVTPDSLRIAE
                     NAILILPLHRELEALREAEDAATRIGTTKRGIGPAYEDKVGRRAIRFMDLADLPALRG
                     KIERLLAHHNALRRGHGLAEVATETVYDHLAAIAPKVLPFMDSVWSLLDGRRREGKRI
                     LFEGAQGALLDIDHGTYPYVTSSNTVAAQAATGSGLGPNAIDYVLGICKAYTTRVGLG
                     PFPTDQMDNSVGKTLGERGREFGTVTGRARRCGWFDAVLVRQAVLTCGIDGLALTKLD
                     ILDGFDEIRVCVRYQLDGREIDYLPAGEQAQARAKPVYETIDGWKEPTARARSWAQLP
                     AQAIKYVRRVEELVGCPVALLSTSPEREDTILMKNPFEL"
     gene            1749..>3014
                     /locus_tag="VFL49_13085"
     CDS             1749..>3014
                     /locus_tag="VFL49_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912961.1"
                     /translation="MADYYPLIARAVGGLDKNTGENRRALYDRARVALVNQLRGVQPA
                     LEESDITRERRALEEAIRKVEAETAKRVRVETSEPEAAEPSLRDRGLRDFRETVAEAE
                     GLGTAAAEAKRAAHATFDAMPDELSAPRQFERQAPIAPVTEPTLADETPPAPPPVPPA
                     SRPAYDDDIYTRPARSYVGLIKIAVIVLVLAGLAGGVYWQRHTIGTMVAQFRSQPAPK
                     PKDTSQPAVRPKIADRIGQSGTTAPDVANAPAAAVAQKVVLYDEDPSDPQGKRYVGSA
                     LWRTETVSPGPGLAPELAIRADIEIPERKLRMTWSLRRNTDKALPASHTIEIMFTLPA
                     DFPEGGIGNVPGVLMKQNEQARGVPLAGLAVKVTNGYFLIGLSAVEVDVQRNIQLLKE
                     RDWFDIPLVYTSGKRAILAMEKGTPGSRAF"
BASE COUNT          563 a          935 c          998 g          518 t
ORIGIN      
        1 gacaagccgg gcttcatcgg caagttctcc tcgaccctcg gcgaagccgg catcaacatc
       61 gccacgtttc acgtcgggcg cgacgcgccg ggcggcaatg ctgtcgccct gatcgagatt
      121 gacggaaaac tgccggaggt cgtgcttgcg aaggtacgcg cgctgccgca ggtgcagcag
      181 gcgaaaccgc tgcatttctg aaagccgcgt aagatcaggc atttgttgct gctgacgggc
      241 ggcgggctcg tcgcggactt tggcttgcct cccacagagc cttcctttat ccgagggtgg
      301 acctggccgg tggcacccgg cttcaagtgt ccgggcgctg gcgcagggaa gggatcctga
      361 tggcgaatgt cgtggttgtc ggatcccagt ggggcgacga aggcaaaggc aagatcgtcg
      421 actggctgtc ggagcaggcc gatatcgtcg ttcgtttcca gggtggtcac aatgccggcc
      481 acacgctggt gattgacggc tcaacctaca agttgtcgct gctgccatcc ggtgtcgtcc
      541 gtgaaggcaa gctttcggtc atcggcaacg gcgtagtgat cgacccgcag gcacttatcg
      601 acgagattgc ccagctcaag aagcaaggca tcgtggtgac gcccgacagc ttgcgcatcg
      661 ccgagaatgc gatcctgatt ctgccgctgc atcgggagct cgaggccctt cgcgaggccg
      721 aggacgccgc gacgcggatc ggcacgacca aacggggaat cggtcctgcc tatgaagaca
      781 aggtcggtcg gcgcgcgatt cgcttcatgg acctcgccga cttgccggcg ctgcgcggca
      841 agatcgaacg gctgctcgcc caccacaatg cgctgcgacg cggtcatggt ctcgccgagg
      901 ttgcgacgga aaccgtgtac gaccatcttg ccgcgattgc gcccaaggtc ttgccgttca
      961 tggactcggt atggtctctc ctcgacggca ggcggcgcga gggcaagcgc attctgttcg
     1021 agggcgcgca gggcgcgctg ctcgacatcg accacggcac gtatccgtac gtgacgtcgt
     1081 cgaacacggt cgcggcacag gcagcgaccg gatccgggct tgggccgaac gcgatcgact
     1141 acgtgctcgg tatctgcaag gcctacacca cgcgcgtcgg tctcggtccg tttccgaccg
     1201 atcagatgga caattccgtg ggcaagacgc ttggcgagcg gggccgcgag tttggcaccg
     1261 tgaccgggcg tgcccgccgt tgcggctggt tcgacgccgt gctggtgcgg caggccgtcc
     1321 tgacctgcgg cattgacggc ctggcgctca ccaagctcga cattctcgac ggtttcgatg
     1381 aaatcagggt ctgcgtgcgc tatcaattgg atggccgcga aatcgactat cttcccgccg
     1441 gtgaacaggc gcaggcgcgg gccaagccgg tctacgaaac gatcgacggc tggaaggaac
     1501 cgaccgcacg agcacgttcc tgggcacagc tgccggcgca ggccataaaa tacgtgcggc
     1561 gtgtggaaga actggtcggc tgcccggtcg cgctgctatc gacaagcccg gagcgtgagg
     1621 acaccatcct gatgaagaat ccgtttgagt tatgattgcc gaaaaaagac acgaaccggg
     1681 gccaagcatg atcatcctag ggagagatcg cgattcggat gccatcatgc accggcgaac
     1741 aaggcgcaat ggctgattat taccccctga ttgcccgggc cgtcggcggc cttgacaaaa
     1801 acacgggcga gaaccgccgc gcgctgtacg atcgcgcgcg ggtggcgctc gtcaatcagc
     1861 tgcgcggtgt ccagccggct ctcgaggaga gcgacatcac ccgtgagcgg cgggcgcttg
     1921 aagaggcgat ccgcaaagtc gaagcggaaa cggcaaagcg cgtgcgcgtc gagacttcgg
     1981 agccggaagc cgccgaaccc tcgctgcgtg atcgcggact gcgcgacttc cgcgagacgg
     2041 tcgccgaggc cgaaggactc ggcaccgccg cggcggaggc aaagcgtgcc gcgcatgcca
     2101 ccttcgatgc gatgccggat gagctgtccg ctccgcgcca attcgagcga caggccccta
     2161 ttgcgccggt caccgaacca acgctcgccg acgagacgcc tcccgcgcca ccgcctgtgc
     2221 cgccggcatc gcgtccggcg tacgatgacg atatctacac acgtccggca cggtcctatg
     2281 tcggcttgat caagatcgct gtcattgtgc tcgtgctggc gggtcttgcc ggcggcgtct
     2341 actggcagcg tcacacaata gggacgatgg tggcgcaatt ccgttcacag cctgcgccga
     2401 agccgaagga tacgagccag ccggcggttc gcccgaaaat tgccgatcgt atcggccagt
     2461 cgggtacgac ggcgcccgac gtggcgaatg cgccggccgc ggcggtggcg cagaaagtgg
     2521 ttctctacga cgaggacccg agtgatccgc aaggcaagcg ttacgtgggt tcggcgctct
     2581 ggcgtacgga gaccgtctca ccggggccgg gccttgctcc ggaacttgcc atccgcgccg
     2641 acatcgagat accggagcgc aagctgcgca tgacgtggtc gcttcggcgc aacacggata
     2701 aggcgctgcc cgccagtcat acgatcgaga tcatgttcac gctgccggcc gattttccgg
     2761 aaggcggcat cggtaatgtg cccggtgtcc tgatgaaaca aaacgagcag gcgcgcggtg
     2821 tgccgctcgc cggcctcgca gtgaaggtca ccaacggata tttcctgatc ggtctctcgg
     2881 cagtcgaagt tgacgtgcag cgcaatatac agctgctgaa ggagcgcgat tggttcgaca
     2941 tcccgctcgt ctacaccagc ggcaagcgcg cgatcctggc gatggaaaag ggcacgcccg
     3001 gcagtcgcgc cttc
//
LOCUS       DASSRL010000640         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_419529, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000640 DASSRL010000000
VERSION     DASSRL010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419529"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VFL49_13090"
     CDS             complement(<1..1392)
                     /locus_tag="VFL49_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7912962.1"
                     /translation="MQLHEQIIDRIAAGELTAGARLPPVRRLAQQLGINHMTVAKAYK
                     DLAEAGFVEGRAGGGTHVRAPYGTGSRRAHGSEPAVAGPLLSERLYELSHAPGVIGFT
                     TNYPAPEPECTREFETCLQSVIAGGLSPYFAYDPPNGRISFRRAIVDYVGRQGLMAAP
                     EDVIVTSGAQQAIELVVRLLVPHGSPVIVERPSYYGVINALRCVGARILEVPVESDGM
                     DIEKLEAHLARHRPRLIYTNPTFQNPTGVTTSLEKRRCILALARKHGVAILEDDHNSE
                     LRFSGSPIPSIRSLSEPGDNVFFVRGFGKVFLPGTRLGYVVTPAHARRPLLALKAHSD
                     LHTNGIMQEAMAGFLARKNYPTFLERMKRVYSAKQRKLIASLSAGMPPGTLIGHPDGG
                     LSLWVTLPEGTEVSELYYRAVRRGVAFVAGEVFYASHVNPRTLRVSFGLLGDDVLDDG
                     VARLCSVAKDLMRP"
     gene            1722..>2546
                     /locus_tag="VFL49_13095"
     CDS             1722..>2546
                     /locus_tag="VFL49_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7912963.1"
                     /translation="MAVAKGLKVVGYLDCAGGGQVVVRNNVAYVGHVKPPNGTTIIDV
                     ADPAKPRELAQIKVPEGTLSHKVRVENGVMLVNREDLPIGRIDPEFKGGLEVFDVSNP
                     SKPTRITTWASRGMHRFTFDGRYVYGSPEFDGYLGNVVSIIDFQNPEKPQEVGRWWMP
                     GQWAAGGEKPTWEGTAHRCHHPMRVGNRLYTSYWHGGFVILDIDDITKPKFVSGLDWS
                     PPFPWPTHTALPVPFPLRGRRVMLVADEDVVRLPGPTPPAFLWLVDITDETRPIPFA"
BASE COUNT          496 a          809 c          782 g          459 t
ORIGIN      
        1 cggccgcatc aggtctttcg ccaccgagca aagcctcgcc acgccgtcgt cgagcacatc
       61 gtctcccagc aatccgaagc tcacgcgcag cgtgcgcgga ttgacgtgcg aggcgtaaaa
      121 aacctctccc gcgacgaagg cgacaccacg gcggacggcg cgataataca gctcggagac
      181 ctcggtccct tccggcagcg ttacccacag gctcaggccg ccgtccggat gcccgatcag
      241 ggtgccgggc ggcatgccgg cggacaggct ggcaatcagc ttgcgctgct tggcggagta
      301 gacccgtttc atccgttcaa gaaacgtcgg gtagttcttg cgagccagga agcccgccat
      361 cgcttcctgc atgatgccgt ttgtgtgcag gtcactgtgt gccttgagtg caagcaaagg
      421 ccgccgcgcg tgcgcgggcg tgacgacata accgagacgc gtccccggca ggaacacctt
      481 tccaaagccg cggacaaaga aaacgttgtc tcccggttcc gacaacgaac gaatggaagg
      541 gatcggcgac ccggaaaagc gcagttctga attatgatcg tcttcgagaa tcgccacgcc
      601 gtgcttgcgc gcgagcgcga gaatgcagcg acgcttttcg aggctcgtcg tcacgcccgt
      661 cggattctgg aaggtcggat tggtatagat caggcgcggc ctgtgccgtg ccaaatgggc
      721 ctcgagtttt tctatgtcca taccatcgga ttcgaccggt acttcgagga tccgcgcgcc
      781 gacgcaccgc agagcgttga tcaccccgta atacgaaggt ctttcgacga tgaccggcga
      841 tccatgcggc accagcagcc ggacgacgag ttcgatcgcc tgctgggcgc ccgaggtcac
      901 gatcacgtct tccggcgcgg ccatcaaccc ttgccggccg acatagtcga cgatcgcgcg
      961 ccggaaactg atgcggccgt tcggcggatc gtaggcaaag taaggcgaga ggccgcccgc
     1021 aattaccgac tgcaggcacg tctcgaactc ccgggtgcat tccggctccg gcgccggata
     1081 gttggtggtg aacccgatga cgccgggcgc atgcgagagc tcataaagac gttcggacaa
     1141 cagcggcccc gcgacggcgg gttccgatcc atgtgcgcgc cgggaaccgg ttccgtatgg
     1201 cgcccgcacg tgcgtaccac cgcccgcgcg gccttcgaca aagccggcct ccgcgaggtc
     1261 cttgtaggcc ttcgccaccg tcatatgatt gatgccgagt tgctgggcga ggcgccgcac
     1321 cggcggcaag cgcgcgccgg ccgtcagttc gcccgcggcg atgcggtcga taatctgctc
     1381 gtgcagttgc atgaaaagcg gccggccatt gcctttcgaa agcgaaatcc caaacgcgct
     1441 cggctcgatt gcccgccgcg gatgcctttg gccgtttgcc attcgacgcc actcaagaca
     1501 aaataccttg tatgattttt tgtattactg cgagcagacg ggagcaagag aaattttgca
     1561 cgaaatgctg gagaaaatac gacgctctct ctcgctctca gaaattcgaa acacgaacag
     1621 attgaccgcc atacaaaccg cattgtagcg ttttgatcgc agatgagcgg atgttgttcg
     1681 cgctctgcgt ggtgtgtgga cgttgacgcg gggtgtggcc gatggccgtt gcgaaagggt
     1741 tgaaggtcgt cggctatctc gactgtgcgg gcggcgggca ggtggttgtt cgcaacaacg
     1801 tcgcctatgt cggacacgtc aagccgccaa acggcacaac catcatcgac gtcgccgatc
     1861 ccgcgaagcc gcgcgaactt gcgcagatca aggtgcccga aggaacgctg tcgcacaaag
     1921 tgcgcgtcga gaacggcgtc atgctggtca accgcgaaga cttaccgatc ggccgcatcg
     1981 atccggaatt caagggcggc ctcgaggtct tcgacgtcag caatccgtca aagccgacgc
     2041 gcatcaccac atgggcgtcg cgcggcatgc accgcttcac cttcgacggc cgttacgtct
     2101 acggctcgcc ggagttcgac ggttatctcg gcaatgtcgt ctcgatcatc gatttccaga
     2161 atccggaaaa accgcaggaa gtcggccgct ggtggatgcc gggacaatgg gccgccggcg
     2221 gcgaaaagcc gacgtgggaa ggcaccgcgc accgctgcca tcatccgatg cgcgtcggca
     2281 accggctcta cacgagctac tggcacggcg gcttcgtcat tctcgacatc gacgacatca
     2341 ccaagccgaa atttgtctcg ggcctcgact ggagcccgcc cttcccgtgg ccgacgcata
     2401 ccgcgctgcc ggtgccgttt ccgctgcgcg gacggcgcgt catgctggtc gcggacgaag
     2461 atgtcgtgcg cctccccggc cccaccccgc cggccttcct gtggctggtc gatattaccg
     2521 acgagacaag gcctatcccc tttgcc
//
LOCUS       DASSRL010000641         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1626827, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000641 DASSRL010000000
VERSION     DASSRL010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626827"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="zapE"
                     /locus_tag="VFL49_13100"
     CDS             complement(<1..940)
                     /gene="zapE"
                     /locus_tag="VFL49_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HET7912964.1"
                     /translation="MSETFSTRYAALVAAAKIEADPGQAVLGRRLAALEQRIDQRRLA
                     RKSSSLGWLFGKREQAGAPLKGLYVYGEVGRGKTMLMDLFFETSAVVRKRRVHFHEFM
                     ADVHERVHVYRQEIKNGEASEHDPIRRAAAAIAEETWLLCFDEFHVTDIADAMILGRL
                     FTRLFELGVVMVATSNLAPGELYKSGLNRALFLPFIALLERHCEVVRLEARIDFRLEK
                     LTGVPTWHVPDDAKADAALDQAWRRLAGGHAGMPQELTVKGHTVRIPKAAMGVARISF
                     DDLCAQPLAASDYLKIAHEFHTVIVDHIPVMDYAQRN"
     gene            1193..1768
                     /locus_tag="VFL49_13105"
     CDS             1193..1768
                     /locus_tag="VFL49_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7912965.1"
                     /translation="MDEPLPDGGVIRKLWIGETDAYRDHLLRLDRESRNRRFSGAVSD
                     EFIAQHAASAGGFGAVLHGFFVDGVLRGAAELRSMGSMLARDGEAAFSVELPWQSHGV
                     GTVLLERTLLSARNRGIKLLRMHCLADNRRMQQLASKFEADLKFDFGSVVGEVDPPRF
                     TLLSLTREVATDAHSVATALLDVQSRLFNPA"
     gene            complement(1769..2305)
                     /gene="thpR"
                     /locus_tag="VFL49_13110"
     CDS             complement(1769..2305)
                     /gene="thpR"
                     /locus_tag="VFL49_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7912966.1"
                     /translation="MPRLFTGVEIPADVGQALATLRGGLPGARWIDPENYHLTLRFIG
                     DVDDDIAHEVASLLGRVKRGPFELHMDGLTFFGGRKPRAVVASVAPAQPLLDVQAEHE
                     RLMQRIGLEPEGRKFTPHVTLARLRESSSRQVAEYLSARGYFRTSPFRVSRFVLFSSR
                     ASVGGGPYVVEAGYPLYG"
     gene            complement(2319..2921)
                     /locus_tag="VFL49_13115"
     CDS             complement(2319..2921)
                     /locus_tag="VFL49_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HET7912967.1"
                     /translation="MLVGVASAADKSVHVVVLGDSLSAGFGLPADATFPAKLAKALSE
                     KGVAVKITNAGVSGDTASAGLARVGWSVPEGTDAVILELGANDALRGLDPKATKASLD
                     AILRKLADRRIPVLLAGMQAPRNLGPDYVRDFDAIYPALASTHPVVFYQFFLEGVAAD
                     PKLNQADGMHPNAAGVDVIVGRILPWVEELIVRARAARAS"
     gene            3019..3717
                     /locus_tag="VFL49_13120"
     CDS             3019..3717
                     /locus_tag="VFL49_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7912968.1"
                     /translation="MTNSSQAGPAIALAGVNLSLGQGAARVHILKDIDLHIGSGEAIG
                     LVGPSGSGKSTLLMVMAGLERADAGTVTVAGENLGELDEDGLARFRGRNVGIVFQSFH
                     LIPTMTAIENVAVPLELAGMADAHAQARDELTAVGLSDRLDHYPAQLSGGEQQRVAVA
                     RALAPNPAIIVADEPTGNLDEDTGKQIIELLFAGHARRKSTLVLVTHDPALAARCARV
                     VRLRSGRVDGAGVS"
BASE COUNT          765 a         1149 c         1203 g          721 t
ORIGIN      
        1 cgttcctctg cgcgtagtcc atcaccggaa tatggtcgac gatcacagta tggaactcgt
       61 gggcgatctt gaggtaatcg ctcgccgcga gcggctgcgc acatagatcg tcaaacgaaa
      121 tccgcgccac gcccatcgca gctttcggta tacgtaccgt gtgtcctttg accgtcagtt
      181 cctgtggcat gcccgcgtgg ccgccggcaa gccgccgcca ggcctggtca agcgcggcgt
      241 cggctttcgc gtcgtcgggt acatgccatg tcgggacgcc ggtgagcttc tccagccgga
      301 aatcaattcg cgcctcaagg cggacaacct cacagtggcg ctcgagcaat gcaatgaaag
      361 gcaggaaaag ggcacggttg agcccgcttt tgtaaagctc gcctggcgcg aggttcgacg
      421 tcgcgaccat gaccacgccg agctcgaaca gccgcgtgaa caagcggccg agaatcatgg
      481 cgtcggcgat gtctgtgaca tggaattcat cgaaacaaag aagccaggtt tcctcagcga
      541 tggcggccgc cgcgcgtcga atgggatcgt gttcgctggc ctccccgttc ttgatttcct
      601 gccggtagac gtgaacgcgt tcatgcacgt cggccataaa ctcatgaaaa tgcacacggc
      661 gtttgcgcac cactgcgctg gtctcgaaga agaggtccat gagcatcgtc ttgccacggc
      721 cgacctctcc gtagacgtaa agtcctttaa gtggagcgcc ggcctgctca cgctttccga
      781 agagccagcc aagcgacgaa gacttgcgcg caagccggcg ctgatcgatg cgctgttcga
      841 gcgcggccaa tcgccggccc agtaccgcct gaccgggatc agcctcgatc tttgcggccg
      901 cgacgagtgc cgcgtagcgt gtgctgaagg tttcggacat gaggccaagc gaagatgtga
      961 agaatgggaa gaataggaag gcgaggggtc cgacacctat ggcgaacgag gaactctgta
     1021 aatcagagct ggggtcacgt tcctcttatt gcgcacaaag cctgcggtaa tcctctggta
     1081 gctatgcgtt ttttgcgggc aagggcttcc gcagacggcg tgcagtcgaa tttttgctgc
     1141 aatgcaccat atgttctgaa acgctgcgga tcgatcgccg aggagaactg atatggatga
     1201 gcctttgccg gacggcggcg tgatccgcaa gttgtggatc ggtgagaccg acgcatatcg
     1261 tgatcatctg ctccgcctcg accgcgaaag ccgcaatcgg cggttttccg gcgcagtctc
     1321 ggatgaattc atcgcacaac atgcggcctc ggcgggcggc ttcggcgcgg tgctgcatgg
     1381 cttctttgtc gacggcgtgc tccggggtgc ggccgaactc cgttcgatgg gctccatgct
     1441 tgcgcgcgac ggtgaagccg ctttcagcgt cgagttgccg tggcagagcc acggcgtcgg
     1501 cactgtattg ctggaacgta cgcttttgag cgcacgcaat cgcggcatca aattgctgcg
     1561 gatgcattgt ctcgctgata accggcgaat gcagcagctg gccagtaaat tcgaagctga
     1621 cctcaaattc gatttcggca gcgttgtcgg ggaagttgat cctccccgtt tcaccttgct
     1681 gtcactcacg cgcgaagtcg ccactgatgc ccacagcgtc gctaccgccc ttctcgacgt
     1741 gcagtccagg ctgttcaatc cggcatgatc agccgtaaag cggatagccc gcctcgacta
     1801 catagggtcc gccgccgaca gacgcccgcg acgagaacag cacaaagcgc gaaactcgga
     1861 acggcgaggt ccggaaatag ccgcgggcgg aaagatattc ggcgacctga cggctggacg
     1921 attcgcgcaa gcgcgccaac gtgacgtgcg gcgtgaattt gcggccttcc ggctcgagcc
     1981 cgatgcgctg catcagacgt tcatgctccg cctgaacgtc gagcaagggc tgcgccgggg
     2041 caacgctggc gacgaccgcg cgtggcttac gtccgccgaa gaaagtcagt ccgtccatgt
     2101 gcagttcgaa ggggccgcgt ttcacgcgtc cgagcaagga agcaacctca tgggcgatgt
     2161 cgtcatcgac atcgccgatg aaccgcaagg tcaggtgata attttcggga tcaatccacc
     2221 gggcgccagg cagtccgccg cgcaaggttg ccaaagcctg tccgacatca gcaggaattt
     2281 caacacctgt gaagagacgc ggcatgacca gatttccatc acgaggcgcg cgcggcgcgc
     2341 gctctgacaa tcagctcttc aacccatggc aggatgcgcc ccacgatcac gtcgacgccg
     2401 gcggcattag gatgcatgcc gtctgcctga ttcaatttgg gatctgcagc cactccctca
     2461 aggaagaatt gataaaagac gaccgggtga gtcgaggcaa gcgcaggata gattgcgtcg
     2521 aaatcacgca cgtaatccgg cccgagattg cgtggcgcct gcatgccggc gagcaacacg
     2581 ggtatgcggc gatcggcaag cttgcgcagg atcgcatcga gtgaagcctt cgtggccttc
     2641 ggatcaaggc cacgcaaggc atcattggcc ccaagctcca ggatgacggc atcagtccct
     2701 tccggaacgg accatccgac gcgggcgagc cctgcggacg ctgtatcgcc ggacacgccg
     2761 gcatttgtaa ttttgacggc gacacctttt tcgctgagtg cctttgccag cttggccggg
     2821 aatgtcgcgt cggccggcaa gccgaagccc gcgctcagcg aatcgccgag aacgacgaca
     2881 tgaaccgatt tgtccgccgc actcgcgacg ccgacaagca cgcatacaca aaacaaagtg
     2941 agcagcagac ggcatgcaag gcgctggacc cggatggggt tcctcccata tgagcgaggc
     3001 tgcggaagat gaggtcccat gacgaatagc tcccaggcgg gcccggccat tgcgcttgcc
     3061 ggtgttaatc tctcgctcgg gcagggggcg gcccgtgttc atatcctcaa agatatcgac
     3121 ctgcatatag ggagcggcga ggcaattggc ctggttgggc catccggttc cggcaaatcg
     3181 acgttgctga tggtgatggc cggcctcgaa cgcgccgacg cagggacagt gacggtcgcc
     3241 ggggaaaacc tgggcgagct cgatgaggat ggtcttgccc gttttcgtgg ccggaatgtg
     3301 ggaatcgttt tccagtcgtt ccatctgatc ccgacgatga cggcgatcga aaacgttgcc
     3361 gtgcctttgg aattggccgg catggccgac gcgcatgccc aggcgcgcga cgaactcacg
     3421 gccgtcggat tgagcgaccg gctcgaccac tatccggcgc aactgtcggg cggcgagcag
     3481 cagcgggttg cagtggcgcg ggcgctcgcg ccaaaccccg cgatcattgt tgcagacgaa
     3541 ccgaccggaa acctggatga ggacacgggc aagcagatta tcgaacttct gtttgccggg
     3601 catgccagac gtaagtccac gctggtgctc gtcacgcacg acccagcact cgccgcccgg
     3661 tgcgcccgcg tcgtgcgtct gcggtcgggc cgcgtcgacg gtgcgggcgt atcgtgatca
     3721 agacggcaca atccaacagt cgatggctgc ccctgcgctt tgcgttgcgc gaattgcgcg
     3781 ccggtgttcg tggcttctac gtcttcatcg cctgcatcgc gctgggcagc atggcgat
//
LOCUS       DASSRL010000642         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_839172, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000642 DASSRL010000000
VERSION     DASSRL010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839172"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFL49_13125"
     CDS             <1..357
                     /locus_tag="VFL49_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7912969.1"
                     /translation="AAMGSDFNQLSGEDITALGFMAHGGHGCISVTSNVAPRLCAEFQ
                     AACLKGDFAAALKLQDELAPLHINLFIETSPAPIKYAMSLIGKCASTVRLPMVPASEK
                     AQTAVRQAMVHAGLIN"
     gene            complement(447..1337)
                     /gene="dapA"
                     /locus_tag="VFL49_13130"
     CDS             complement(447..1337)
                     /gene="dapA"
                     /locus_tag="VFL49_13130"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7912970.1"
                     /translation="MTARTSFRGSFTALVTPFKNGSLDEKAFRDIVEWQIAEGTNGLV
                     PVGTTGESPTLSHDEHKQVVEWCVDQADGRVPVIAGSGSNSTAEAIELSRHAEKAGAD
                     AVLIVTPYYNKPTQDGLYEHYKAINDAIGIPIIIYNIPGRSIVDMSVETMARLFELKN
                     VAGVKDATANMTRVSQQRAVMGSDFNQLSGEDITALGFMAHGGHGCISVTSNVAPRLC
                     AEFQAACLKGDFASALKLQDKLAPLHINLFVETSPAPIKYAMSLIGKCANTVRLPMVP
                     ASEKAQIAVRQAMVHAGLIN"
     gene            1763..3868
                     /locus_tag="VFL49_13135"
     CDS             1763..3868
                     /locus_tag="VFL49_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7912971.1"
                     /translation="MRQAAPLPQKRPEPDGNGLTAFAQANVGLHGALFESRAEFKPLA
                     RPASGPFAIAPHVATSASDVAAVKRVIEAARKGKDADADTAQASISDPVARKLAEWVI
                     LRSDNTNPSFQRYAAFVQNNPAWPHVPLFRRRAENALWNDGVDASAVRAFFANKSPST
                     AKGRYSLARVLLAQGDREGAAALVRHAWRYDDCSAEVEKKVLEMFGGMLTAADHKVRM
                     ETRFYAGDAAAGLRAAERLGGNDLVIARARAAVLQKARNAKALLDAVPAAARDEPGYI
                     FARVQWLRQNNKPEEAGKLILTAPKDPQALVNLDQWWLERRLLVRKLLDEHNPQAAYN
                     IAREAAPPMRGVYRVDAHFTAGWVALRYLHNPKAAAQHFAHIVEGTHNPHALSRGGYW
                     QGRAAEAMGQNAQARVFYEMAAQYVATYYGQLARARLGLKDLGLRQPPSFTPQELNVL
                     GNLEVVRAAAILYELGERDMLASIYAELGESATDVAGMAMLGELAARHGDGRAMLLLG
                     EAAYRRGLPLEHYAYPTVGLPDYSPIAPPVEPAVTYSIARQESHFNQKVVSIAHAMGF
                     MQVTPAAGIDTAAKYKVKYSRERLLKDPVYNMQMGAAELANLFGGYNGSYILTFAGYN
                     AGRGRVKQWIAAYGDPRDPNVDPVDWVERIPISETRNYVERIMENLQVYRARFGGGTK
                     LLIEADIRRGGPAHMTTSR"
     gene            3916..>4684
                     /locus_tag="VFL49_13140"
     CDS             3916..>4684
                     /locus_tag="VFL49_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7912972.1"
                     /translation="MLASTAISTFITAPDGLRLHARCYGRISAPGIPVVCLPGLARTA
                     ADFEVLAAALSRDGPHPRRVIALDYRGRGLSQYDRNPANYSFPIELADVLAVVTALES
                     LPAVFIGTSRGGILTMLLAAVRPSAIAGVVLNDIGPVIEPQGLMRIKGYVGKLPQPRT
                     FEEGAEILRRLFHSQFPRLGPEDWLAGARRTFKEENGRLVTTYDPKLAATMAGIGPEQ
                     PLPALWKEFDALANVPVMVIRGANSDILSPATVGQMRA"
BASE COUNT          847 a         1500 c         1453 g          884 t
ORIGIN      
        1 gccgccatgg gctccgactt caatcagttg tcgggcgaag acataacggc gcttggcttc
       61 atggcacatg gcgggcacgg atgcatatca gtgacatcga acgtggcgcc gcggttgtgc
      121 gctgaatttc aggcggcctg cctgaagggg gatttcgctg ctgcactcaa attgcaggac
      181 gagctggcgc cgctgcacat caatctgttc atcgaaacca gcccggcgcc gatcaagtat
      241 gcgatgtcgc tcatcggcaa gtgcgccagc acggttcggt tgccgatggt gcctgccagc
      301 gagaaagcac agaccgccgt gcgccaggcg atggtgcatg ccgggctgat caattagagc
      361 ccgtcatggc cgggcttgtc ccggccatcc acgccttctg ctgacgaaga cgtggatgcc
      421 cggcacaagg ccgggcatga cagcctctaa ttgatcagcc ccgcgtgcac catcgcctgt
      481 cgcacggcga tttgcgcctt ttcgctggcc ggcaccattg gcagccgtac cgtgttggcg
      541 cacttgccga tgagcgacat cgcatacttg atcggagccg ggctggtttc cacgaacagg
      601 ttaatgtgca gcggcgcgag cttgtcctgc agtttaaggg cactggcaaa atcgcccttg
      661 aggcaggccg cttgaaattc agcgcacaac cgcggcgcta cgttcgacgt caccgagatg
      721 catccgtgcc cgccatgcgc catgaagcca agtgccgtta tgtcttcgcc cgacaactga
      781 ttgaagtcgg agcccatgac agcgcgctgc tgggaaacgc gtgtcatgtt ggcggtcgca
      841 tccttcacgc ccgcaacgtt tttcaactcg aaaagacgcg ccatcgtctc gaccgacatg
      901 tcgacgatcg accgtccggg gatgttgtag atgatgatgg ggatcccgat tgcgtcgttg
      961 atcgccttgt agtgctcgta caagccgtct tgcgttggct tgttgtagta gggcgtcacg
     1021 atcagcaccg cgtcggcgcc ggccttctcg gcatggcgcg aaagttcgat cgcctctgcg
     1081 gtggaattgg agccggaacc cgcgatgacc ggcacgcggc cgtcggcctg gtcgacgcac
     1141 cactcgacca cctgcttatg ttcgtcgtgc gagagcgtcg ggctttcccc ggtggtcccc
     1201 accgggacca ggccattcgt gccttcggca atctgccatt cgacgatgtc gcggaacgcc
     1261 ttttcgtcga gcgagccatt tttgaacggt gtgaccagcg cggtaaaaga gccccgaaag
     1321 ctcgtcctgg cggtcatggc gcgacttctc cacaaaaggc gttcaagcac cattgatatc
     1381 gggtcggagg ggccgacgaa agccctccaa ccctgccgga atggtggaac tcagcctgcg
     1441 acatcgtgtt tttgccgtct tatcggccaa agtgccggcg gatgaaattt ttcggtatca
     1501 ttcatcaata gatttggcct aatggactgg ccaatctgct gtttcaggac tgagtcgggc
     1561 ggatcgaagg acaatcgaag gacacgtgcg gggtttgatg ggcgcgactg gccgcagccg
     1621 ctggggcttg tttgtggcaa cgattgcagc ctgcgcattg tgcgcggagg cggcgctgcc
     1681 ggccacccaa aaagtgcctc tgccgcgtcc gcggcctcct gtgccaccgt gcaagcaagc
     1741 cgccgcgccc aagccagcgc ctgtgcggca agcggcgccc ttgccgcaga agcggccgga
     1801 gcccgacggt aacgggctca cggcctttgc gcaggccaat gttggcctgc acggcgcatt
     1861 gttcgagagc cgggctgaat tcaagccgct tgcgcgcccg gcttcgggtc cctttgcgat
     1921 tgcgcctcac gtcgccacgt cggcgagcga cgtcgccgcg gtaaagcgtg tgattgaagc
     1981 cgcacgcaaa gggaaggatg ccgacgccga cacggcacag gcgagcatct ccgacccggt
     2041 tgcccgcaag ctggccgaat gggtcatcct gcggtccgat aacaccaatc cctcattcca
     2101 gcgctacgcc gcgttcgtcc agaacaatcc ggcctggccg catgtgcccc tgttccgccg
     2161 ccgcgccgag aacgcgctct ggaacgacgg cgtcgatgcg tcggccgtgc gcgctttctt
     2221 tgccaacaag tcaccgtcaa cggccaaggg gcgctactcg ctcgcgcgag tcctgctcgc
     2281 tcaaggcgat cgcgagggag cggcggcgct ggtcaggcat gcctggcgct acgacgattg
     2341 cagcgccgag gttgagaaga aagtgctcga gatgttcggc ggcatgctca ccgccgccga
     2401 tcacaaggtg cgcatggaga cccgcttcta cgccggcgac gccgccgccg gcctgcgcgc
     2461 ggccgaacgc ctcggcggca acgatcttgt gatcgcccgg gcacgtgccg cggtcctgca
     2521 aaaagcgcgc aatgcaaaag ctttgctcga cgccgtgccg gcggccgcgc gtgatgagcc
     2581 gggatatatc tttgcccgcg tgcagtggct gcgccagaac aacaagccgg aagaagccgg
     2641 aaagctcatt ctgacggcgc cgaaagatcc gcaggccctc gtcaatctcg accagtggtg
     2701 gctcgagcgc cgtttactgg tgcgcaagct gctcgatgag cataatccgc aggcggcgta
     2761 caacatcgcg cgcgaagcgg cgcctccgat gcgcggagtt tatcgcgtcg acgcccactt
     2821 cacggccggc tgggtggcgc tgcgctatct gcacaatcca aaggctgctg cacagcattt
     2881 cgcgcacatc gtcgaaggca cgcacaatcc gcacgcactt tcgcgcggcg gttattggca
     2941 gggccgcgcg gccgaggcga tgggtcaaaa tgcgcaggcc agagtatttt acgagatggc
     3001 tgcgcagtat gttgcaactt attacgggca gctcgcgcgc gcccgcctcg ggctcaagga
     3061 tctcgggctg cgccagccgc cttcctttac cccgcaggaa ctgaatgtgc tgggtaacct
     3121 tgaggtcgtg cgcgcagcgg caatcctcta cgagctcggc gagcgggaca tgctggcgtc
     3181 gatctatgcg gagcttggcg agagcgcgac ggatgttgca ggaatggcca tgctgggcga
     3241 actcgcggcc aggcacggag acgggcgggc aatgctgctg ctcggcgagg ctgcctaccg
     3301 tcgcgggctt ccgctcgagc actacgcata tccaaccgtg ggcctgcccg attacagccc
     3361 gatcgctcct cctgtcgagc cggccgtcac ctattcgatc gcgcgtcagg agagtcactt
     3421 caatcaaaag gttgtctcaa tcgcccatgc gatgggtttc atgcaggtaa cgcctgctgc
     3481 cggcatcgac accgccgcca aatacaaggt gaaatacagc cgcgagcgtc tcctcaagga
     3541 cccggtctat aacatgcaga tgggagcggc cgagctcgcc aatctgttcg gtggctataa
     3601 cggttcttac atcctcacct ttgccggcta caacgccggg cgcggcaggg tgaaacaatg
     3661 gatcgctgcc tatggcgacc cgcgtgaccc gaacgtcgat ccggttgact gggtcgagcg
     3721 cattccaatc tccgaaacac gcaactacgt cgagcggatc atggagaatc tgcaggttta
     3781 ccgggcgcgg ttcggcggcg gcaccaagct cctgatcgaa gccgacatca ggcgcggcgg
     3841 accggcgcat atgacgacgt cgcgttagcc gcacctgcca tccgcgcccg cagacacatt
     3901 ctcgctcgca caccaatgct agcttccacg gcaatctcca ctttcatcac cgcccccgat
     3961 ggcctgcgcc ttcacgcgcg ctgctacggc cgcatcagtg ctccgggcat tcctgtcgtg
     4021 tgcttgcccg ggctcgcccg cactgcggcc gattttgagg ttctggcggc cgcgctttca
     4081 cgtgatggtc cgcacccgcg ccgcgtcatc gcgctcgact accgtggccg tggcctgtcg
     4141 caatacgatc gcaacccggc gaactattcg tttcctatcg aactcgccga cgtcctggcc
     4201 gtcgtcacgg cactggagtc acttccggcc gtttttatcg gcacgtcccg cggcggtatt
     4261 ctgacgatgc tgctggctgc cgtccggccg tcggcgatcg ccggcgtcgt gctcaacgac
     4321 atcggtccgg tcatcgagcc gcaagggctc atgcgcatca aaggctacgt cggcaaattg
     4381 cctcagccgc gaacctttga ggaaggcgcc gagatcctgc gccgcctgtt ccactcgcaa
     4441 tttccccgcc tcggtccgga ggattggctg gccggggcgc gccgcacatt caaggaggaa
     4501 aatggccgcc tggtgacgac ctacgatccg aaacttgcgg ccaccatggc ggggattggg
     4561 cccgaacagc cgctacccgc gctgtggaaa gaattcgacg cacttgcgaa cgtaccggtt
     4621 atggtcatac ggggcgcgaa ttctgacatt ttatcacctg ccacagttgg acagatgcgc
     4681 gctc
//
LOCUS       DASSRL010000643         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_576894, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000643 DASSRL010000000
VERSION     DASSRL010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576894"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /gene="erpA"
                     /locus_tag="VFL49_13145"
     CDS             complement(<1..272)
                     /gene="erpA"
                     /locus_tag="VFL49_13145"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET7912973.1"
                     /translation="MNAVTEQMPDPLVFTDAAAAKVKSLIDEEGNPNLKLRVFVQGGG
                     CSGFQYGFTFDEEAGDDDTRMEKGGVTLLVDPMSLQYLSGAEIDYQ"
     gene            complement(310..720)
                     /locus_tag="VFL49_13150"
     CDS             complement(310..720)
                     /locus_tag="VFL49_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7912974.1"
                     /translation="MFGGQGGNKPKNSIECLIGAGTTIEGNITFAGGLRVDGRVRGDV
                     ISADGKPGTLVLSEQAQIEGEIRVSHVVINGTVVGPVHAAEYVELQAKANVTGDVYYK
                     TLEIQLGAVVQGRLVYQNDSKSDKVVQFKPATAD"
     gene            complement(731..1423)
                     /locus_tag="VFL49_13155"
     CDS             complement(731..1423)
                     /locus_tag="VFL49_13155"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HET7912975.1"
                     /translation="MRAHVPWYWRWLGYVTIGAVVVGVGWTTYDYGMELAGFRQSEAE
                     RAHAKLMEQIKQRESENAELRTQVTAAERQMQIERATYGDLAKQVKSLSEENAALRED
                     LAFFQSLMTADAKEGGALSVNRFRVQSESLPGEYRYRLLLVQTGQRVKEFQGKLQFVL
                     NLQQGDRKFVLTLPAESEKDAKEYQLSFKFFQRVEGTFKVAPEAVVKSLQLRVFENGS
                     VAPKLLQTVSIS"
     gene            complement(1487..2527)
                     /gene="argC"
                     /locus_tag="VFL49_13160"
     CDS             complement(1487..2527)
                     /gene="argC"
                     /locus_tag="VFL49_13160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7912976.1"
                     /translation="MAKNRIKVGIVGGTGYTGVELLRLLALHPHCELTAITSRKEAGM
                     GVAEMFPNLRGHVKLKFSDPAKAGLKDCDLVFFATPNGVAMGEARALVDAGVRIIDIA
                     ADFRIRDVAVWEQWYGVKHACPELVAEAVYGLPEVNRDKLREARVVANPGCYPTAVQL
                     GFLPLIEAGLADLDHLIADAKSGVSGAGRRAEVHTLLAEASDNFKAYGVTGHRHHPEI
                     AQGLERAAGRPVNLVFVPHLVPMIRGIHATLYIRLTKKADLQALYEKRYAKEGFVDVM
                     PPGSHPDTRSVRGANMCRIAVHPPKSGDTAVVLSVIDNLVKGASGQAVQNMNVMFGFP
                     ETAGIEQIALLP"
     gene            complement(2703..3098)
                     /gene="rpsI"
                     /locus_tag="VFL49_13165"
     CDS             complement(2703..3098)
                     /gene="rpsI"
                     /locus_tag="VFL49_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7912977.1"
                     /translation="MQRQQHHYGTGRRKTAVARVYIKAGKGDIIVNGKPVDQFFSRET
                     GRMIVRQPLTVTNTLTTFDIQVNVDGGGESGQAGAVRHGITRALIQHDATLKTALKKA
                     GLVTRDAREVERKKVGLHKARRRGQYSKR"
     gene            complement(3114..3542)
                     /gene="rplM"
                     /locus_tag="VFL49_13170"
     CDS             complement(3114..3542)
                     /gene="rplM"
                     /locus_tag="VFL49_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7912978.1"
                     /translation="MKTFTAKPETVQHGWYVVDASGKVLGRLASEIARRLRGKHKPEY
                     TPHVDTGDYIVVVNVDKLRVTGRKAQNKIYHRHTGYPGGIYSSSFAKLQARHPDRVLK
                     LAVKGMLPKGPLGYAMIKKMKTYVGADHPHTAQQPKQLEI"
BASE COUNT          653 a         1252 c         1067 g          807 t
ORIGIN      
        1 tgctggtagt cgatctccgc tccgctcaaa tactgcaggc tcatgggatc gacgagcagc
       61 gttactccgc ctttttccat gcgagtgtca tcgtcgccgg cttcctcgtc gaaggtaaag
      121 ccgtactgaa accccgagca gccgccgcct tgcacgaaga cgcgcagctt gaggttgggg
      181 ttgccttcct cgtcgatcaa actcttcacc ttcgccgcgg cggcgtccgt gaacaccagc
      241 gggtcgggca tctgttcggt gaccgcattc atgtcagtct cctgctacgt cgataattcc
      301 cggattgggc tagtcggcag tggccggctt gaactgcacg accttgtccg atttgctgtc
      361 attctggtag accagccgcc cctgcaccac cgcgccgagc tgtatctcca gagtcttgta
      421 atacacatct cctgtgacat tggccttggc ctgcagttca acgtactccg ctgcatggac
      481 cgggccgacg acagtgccgt taatcaccac gtgcgatacc cgaatttcgc cctcgatctg
      541 ggcctgctcg gacaacacca gcgtacccgg cttgccgtcc gccgagatca catcaccccg
      601 tacccgcccg tccacgcgca ggcctccggc aaacgtgatg ttgccctcga tggtcgtgcc
      661 ggcaccgatc aggcactcta tggaattctt gggcttgttg ccgccttgtc cgccgaacat
      721 aaagtctccc ctacgaaatg ctcacggttt gcaatagttt aggcgcaacc gagccgtttt
      781 caaagacccg caattggagg ctttttacca cggcttccgg ggccaccttg aatgttcctt
      841 cgacgcgctg gaagaacttg aagctcaact ggtactcctt cgcgtccttc tcgctctcgg
      901 ccggaagcgt tagcacgaac ttgcggtcgc cctgctggag attcagcacg aactgcagct
      961 tgccctggaa ttccttaacg cgctggccgg tctgcaccag caataggcgg tagcggtact
     1021 ccccaggcag ggattcggac tggacacgga agcgattgac gctcagcgcg ccgccttcct
     1081 tggcatcagc ggtcatcaac gactggaaga aggccagatc ctcgcgcaag gcggcgttct
     1141 cctcgctgag cgatttcact tgtttggcca gatcgccgta tgtcgcgcgc tcgatctgca
     1201 tctgccgctc ggcggctgtg acctgcgtgc ggagctcggc attctcgctc tcgcgctgct
     1261 tgatctgctc catcagcttg gcgtgcgcac gctcagcctc gctctgccgg aagcccgcga
     1321 gttccatgcc gtagtcgtaa gtcgtccagc caacaccgac caccactgca ccaatcgtga
     1381 cgtagccgag ccagcgccag taccaaggca cgtgcgcgcg caccgcgact ttaggcgccg
     1441 agatgccaaa tttgcgctgc agcgctcgaa gatactgggc catgcgctag ggcaacaacg
     1501 cgatctgctc tatcccggcg gtctcgggaa atccgaacat gacgttcata ttttgcaccg
     1561 cctgccccga cgcgccttta accaggttgt cgatgacgga cagcacgacc gccgtgtccc
     1621 cgctcttcgg tggatgaacc gcgatacggc acatgttggc cccgcgcacc gaccgcgtat
     1681 ccggatgact gcccggcggc atcacgtcga cgaagccctc ttttgcgtag cgcttctcgt
     1741 acagcgcctg caaatccgcc tttttcgtca ggcgaatgta gagcgtggca tggatgccgc
     1801 gaatcatggg caccaggtgc ggcacgaaca cgagattcac gggccggccg gccgcccgct
     1861 ccagcccttg cgcgatctcg ggatgatgcc ggtgcccggt tacgccgtat gccttgaagt
     1921 tgtccgaggc ctctgcaaga agcgtatgca cttccgccct gcgccccgcg cccgacacgc
     1981 ccgacttcgc gtcggcaatc agatgatcga gatcggcaag gccggcttcg ataagcggca
     2041 ggaaaccgag ttgaacggcc gtcggatagc acccaggatt cgcaaccacc cgcgcttccc
     2101 gcagcttgtc gcgattcact tcgggtaatc cgtacacggc ttccgcgacg agctcggggc
     2161 aggcgtgttt caccccatac cattgctccc acacggcgac gtcgcgaatg cggaaatcgg
     2221 cggcaatatc gatgatccgg acgccggcat ccaccagcgc acgcgcctcc cccatcgcga
     2281 cgccgttggg cgtggcaaaa aacaccagat cgcagtcctt gagccccgct ttggcgggat
     2341 cggagaactt gagctttacg tgcccgcgca aattgggaaa catttccgcg acgcccatgc
     2401 ccgcttcctt gcgggaagta atggcagtga gctcgcagtg cgggtgaaga gcaagcaggc
     2461 gcagcagctc gactccggtg taaccggtgc cgcctacgat cccgaccttg atcctgttct
     2521 tcgccatcgc ttgctcccta tcctgcgcca ttgatgccgg cagcaccagg gcgcatgccc
     2581 tcgttccttc acacctctgc cgaaccagta ggggtgcacg aggggacttc ccctcgttcc
     2641 gaattcttgc tccacagaca acaaagccgc ccgcaggcgg ctttgttgcg ggtgaataca
     2701 gtttaccgct tcgagtactg tcctcgacgt cgggccttgt gcaagccgac cttcttgcgc
     2761 tcgacttcgc gcgcgtcacg cgtcaccagt ccggccttct tcagcgcggt cttgagcgtc
     2821 gcgtcgtgct ggatcagcgc gcgcgtgatg ccgtgacgca ccgcacccgc ctggccagac
     2881 tccccgccgc cgtcgacgtt gacctggatg tcgaacgtgg tcagcgtatt ggtcaccgtg
     2941 agcggctggc gtacgatcat gcgcccggtc tcacgcgaga agaactgatc taccggcttg
     3001 ccgttgacga tgatgtcgcc cttgcctgcc ttgatgtaga cccgtgcgac cgcggtcttg
     3061 cggcggccgg tcccgtagtg atgctgctga cgttgcatgg ccatggcggt ttcttatatc
     3121 tccagttgtt tcggctgttg cgcggtatgc ggatgatccg cgccgacgta agtcttcatc
     3181 ttcttgatca tcgcatagcc gagcggcccc ttcggcagca tgccttttac ggccagcttc
     3241 agcacccggt ccggatggcg tgcttgcagc ttggcgaagc tcgagctgta gatgccgccc
     3301 gggtacccgg tatgacgatg gtaaatcttg ttctgcgcct tgcggcccgt cacgcgcagc
     3361 ttatcgacgt tgaccacgac gatataatcg ccggtgtcga catgcggcgt gtactccggc
     3421 ttgtgcttgc cgcgcagccg ccgcgcgatt tcggatgcca gcctgccgag cactttgccg
     3481 gaggcgtcca cgacatacca gccgtgctgg accgtctcgg gctttgcggt aaacgttttc
     3541 atgacccttg cctggattgg cggaaagggc cgaattctat tgcaaatcag atgcctgtgt
     3601 caaatccccc accctccttt tgaacccgct gcgggggccc ctgacgcgct atttaaccct
     3661 ctccgaaaca ataccttact gtggaatggc tgatcactaa tgccgtggcc gcctggctcc
     3721 tgccgccagg cttcattctt gccgccctgt tcctcgcctg gctggcctgg aatcgacgt
//
LOCUS       DASSRL010000644         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_751943, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000644 DASSRL010000000
VERSION     DASSRL010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751943"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFL49_13175"
     CDS             <1..219
                     /locus_tag="VFL49_13175"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET7912979.1"
                     /translation="ASRRDATWRRLGENAHCFKRQSVHSASHQEIYEAIAARNSERAF
                     DCMYRHLSDVQRHVYTYAFPAEQKRVAE"
     gene            311..880
                     /locus_tag="VFL49_13180"
     CDS             311..880
                     /locus_tag="VFL49_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET7912980.1"
                     /translation="MFGVGASTLLTGLALRRGQAQQTLLAATPHCRDDEDPTPAQTAG
                     PYFTPNSPERRNLVEEGMPGARISLIGFVLDRRCSPVPHALLDLWHCNAAGEYDNRGY
                     RLRGHQFSDAQGRFAFETIVPGLYPGRTRHFHVKVQAPGQDILTTQLYFPDEPQNARD
                     GIFESALLMDVTEDAAVKIGRYDFVLDAG"
     gene            complement(937..2727)
                     /locus_tag="VFL49_13185"
     CDS             complement(937..2727)
                     /locus_tag="VFL49_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7912981.1"
                     /translation="MGSMERRLAAVLIGDVVGYGRLSQIDEEGTRARFDADLHELFEP
                     KIAAHHGRLIKTMGDGLLVEFHSVVDAVRCAIDIQRDKAERNASEAKETCLVYRMGIN
                     LGDVIVEGGDIHGDGVNMAHRLQELADQGGIAISGAVYDQVKTKIGIGYAFLGERSLK
                     NITDPVRLYRVLMDPGAASDKSISVAPNIHDSRPDKPAIAVLPFTNLGGDQDQEYFSE
                     GITDDIITELSRFRNLVVIARNSSFAYKGRSVDIGTIARELGVEYVLEGSIRRAGQRV
                     RISAQLINAASGNHVWAERYERELIDIFSVQDDVSLSIVAALAVELEDEALERARRKP
                     LENLQAYEYWLRGKRLVFLMGKNILEARSHFERAAALDPNFSSAHSGLALTYQMEALD
                     FPRPADFHAAYEKSFQLARRALLLDETNHQAHLALAYGYLYRRDCGQAKKHIERAIKL
                     NPSDGDTLSHAAWLLAMIGEPEAAIRFGEMALKLNPHHPDWYLAFLSTALFAAHRYSE
                     AHVLKSGVPEAFIDSPFFGAATLAHMGRLDEARQWAARAITKLAATPGGELAVAEGRV
                     VGILLDNNPFCRQEDRDHFANGMRKAGVPG"
     gene            complement(2926..>3238)
                     /locus_tag="VFL49_13190"
     CDS             complement(2926..>3238)
                     /locus_tag="VFL49_13190"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HET7912982.1"
                     /translation="IALPAGVSANVPVEAGHNAFAYVFEGTAKIGAGDAAQVVERGQL
                     AVLSLGDAVTVEGGAKGSRLILVAGRPLREPVAKYGPFVMNTQDEIRQAIADYQAGKF
                     V"
BASE COUNT          615 a         1033 c          958 g          632 t
ORIGIN      
        1 gccagccggc gcgatgccac ctggcggcgg ctcggcgaga acgcccattg cttcaagcgc
       61 cagtcggtgc attccgcgtc gcaccaggag atctacgagg cgatcgccgc gcgcaacagc
      121 gagcgcgcct tcgactgcat gtaccggcac ctgagcgacg tgcagcgtca cgtctacacc
      181 tacgccttcc ccgcggagca gaagcgcgtc gcggagtgat cgcaagattg tgactgcccg
      241 ctcgttgcag gcggacatcg actgcgagta cggatagatc atggctttgg tttgtctatc
      301 gcgccgcgcc ttgttcggcg tcggcgcttc gaccttgctc accggcctcg ccctgcgccg
      361 cggccaggcg cagcagacct tgctggccgc aaccccgcac tgccgcgacg acgaagaccc
      421 aaccccggca cagaccgcgg gcccctattt cacgcccaac tcgcccgagc gcagaaacct
      481 ggtcgaggag ggaatgccgg gcgctcggat cagcctaatc gggttcgtcc tggacaggcg
      541 atgcagtccg gtcccgcatg cgctgctcga tctatggcat tgcaatgcgg ccggcgaata
      601 cgacaaccgg ggctaccgcc tgcgcggcca ccagttcagc gatgcgcagg ggcgcttcgc
      661 gttcgagacc atcgtccccg gtctctatcc cggccgcacc cggcatttcc atgtgaaggt
      721 ccaggcgccg gggcaggata tcctgacgac ccagctctat ttcccagacg agccgcagaa
      781 cgcgcgcgac ggcatcttcg agtccgcgct gctgatggat gtgactgaag atgccgcagt
      841 gaagatcggg cggtatgatt tcgtgttgga cgcgggctga gcactacgtc cgccgtctat
      901 gccgcggcgg actgcggctt gaacctgccc gggtagttag ccaggcacac cagccttgcg
      961 catcccgttc gcgaaatgat cacgatcctc ttgacggcag aaggggttgt tgtcgagcag
     1021 aatcccaaca acacggcctt ccgccactgc caattcgccg ccgggcgtgg ccgccagctt
     1081 ggtaatcgcc ctggcggccc actgccgtgc ttcatcgagg cgtcccatat gagcaagggt
     1141 cgcggccccg aagaacggcg aatcgatgaa ggcttcgggt acacccgact tgagaacgtg
     1201 tgcctccgaa tagcggtgcg cagcgaacag ggcggtgctg agaaatgcca gataccagtc
     1261 aggatggtgc gggttcagct tcagagccat ctcgccgaat ctgatggccg cttccggctc
     1321 gccaatcatg gccaggagcc atgctgcatg agacagggta tcgccatcgc tgggattgag
     1381 tttgatggcc cgctcaatgt gcttcttggc ctgaccacag tcacgccgat agaggtagcc
     1441 gtaagccagc gcaaggtgtg cctgatgatt ggtctcgtcg agcagcagtg ctcgccgagc
     1501 gagttgaaac gacttttcgt acgcagcatg gaaatcggcc ggccgcggaa agtccaaagc
     1561 ctccatctgg taggtcagcg ccaatcctga atgagcgctc gaaaagtttg gatccagtgc
     1621 tgccgcccgc tcgaaatggc tgcgggcctc gagaatgttc ttccccatca agaatacgag
     1681 ccgtttgccc cgcagccagt actcgtaggc ttgcagattt tccagcggtt tgcgccttgc
     1741 tctctccaac gcttcgtctt ccagctccac cgccagggcc gccacaatgc tgaggctgac
     1801 gtcatcctgc accgagaaga tgtcgataag ctcacgctcg tagcgttcgg cccagacatg
     1861 gttgccgctc gcggcgttga tgagctgagc ggagatgcgc acccgctgtc cggctcggcg
     1921 gatactgcct tccagcacgt actcgactcc cagctcgcgg gcgatcgttc caatgtcgac
     1981 cgacctgcct ttgtaagcaa aggaagagtt gcgagcgatc acaaccagat tccgaaagcg
     2041 cgacagctcc gtgatgatat cgtcggtgat tccctcggag aaatactcct ggtcctgatc
     2101 gccacccaga ttggtaaagg gcaagacggc gatggcgggc ttgtcaggac gagagtcgtg
     2161 gatattcggc gcgacactga tcgatttgtc cgacgccgca ccaggatcca tcagaactcg
     2221 gtagagccgc actggatcag tgatgttctt cagcgaccgt tcgccaagaa acgcatatcc
     2281 aatacctatc ttggttttca cctgatcgta gaccgcacct gaaatcgcga tgccgccttg
     2341 atcggccagc tcttgcaggc ggtgtgccat attcaccccg tcgccatgaa tgtcgccacc
     2401 ttcaacgatt acatcgccaa gattgatgcc catccgatag acgaggcacg tttcctttgc
     2461 ctcactggca tttcgttcag ctttgtctct ctggatgtct atcgcgcagc gaaccgcgtc
     2521 gactacacta tggaactcga cgagcaagcc gtcgcccatc gtcttgatga gacggccatg
     2581 atgtgccgcg atcttcggct caaagagctc gtggagatct gcgtcgaagc gagcgcgcgt
     2641 accttcttcg tcgatctggc tgagacggcc atagccgacg acatcaccga tcaggacggc
     2701 agcgagtcga cgctccatgc ttcccattca agaggggtca acacagacgc gatcctgcgg
     2761 aattatggtg cataccgtcg gttttggaaa tcggcttgtc ttcctcagcc gatgtcactt
     2821 acgagcgaca tcggcggatt ctgacctgca gataatcggt cttgcaatgg gcttggcagc
     2881 ggatttgcca gcagttctag ggccgttaca ttagtgtgga gcggactaaa cgaacttccc
     2941 cgcctggtag tccgcgatcg cctgcctgat ttcgtcctgc gtgttcatca cgaacggacc
     3001 gtatttggcg accggctcgc gcagcggccg gccggcgacc aggatgaggc ggctgccctt
     3061 ggcgccgccc tcgaccgtca ccgcatcgcc aaggctgagc accgcgagct ggccgcgctc
     3121 caccacctgc gccgcatcgc ccgcgccgat cttcgccgtg ccttcgaaca cgtaggcgaa
     3181 ggcgttgtgc ccggcctcga ccggcacgtt cgccgagacg cccgccggca gcgcgatg
//
LOCUS       DASSRL010000645         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1574380, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000645 DASSRL010000000
VERSION     DASSRL010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574380"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFL49_13195"
     CDS             complement(<1..243)
                     /locus_tag="VFL49_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7912983.1"
                     /translation="MVNGAIQKPAENGPDSTVVRALGGRSIVLVGMMGAGKSSIGRRL
                     AGRLGIPFVDADSEIESAAGMTIPEIFEKHGEPYFRA"
     gene            370..519
                     /locus_tag="VFL49_13200"
     CDS             370..519
                     /locus_tag="VFL49_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7912984.1"
                     /translation="MPSLFRFLVLFGLLGGLAYGAVFSLATFVKYKPREIIVTIPPDK
                     FVKNH"
     gene            541..1488
                     /gene="xerD"
                     /locus_tag="VFL49_13205"
     CDS             541..1488
                     /gene="xerD"
                     /locus_tag="VFL49_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7912985.1"
                     /translation="MSRRPGASDEALIELFLDMLAAERGASENTLTAYRNDLEDLSAH
                     LRASRSTIANATTDNLRKFIASLAERGFKPSSLARRLSAVRQLYRFLYAEGKRGDDPA
                     AVLEGPKRGRSLPKVLSIADVDTLLKTARTSGDNVGQPLLQRLRAARLLCLLEVVYAT
                     GLRVSELVALPAAAARRDQRMLVVRGKGGKERLVPLNKQAKRAMAEYLELRSKAKRDA
                     ESKWLFPSFGEQGHLTRQHFARELKTLGAACGIQPARLSPHVLRHAFASHLLHNGADL
                     RVVQTLLGHADISTTQIYTHVLEERLKKLVRDLHPLADG"
     gene            1584..2540
                     /locus_tag="VFL49_13210"
     CDS             1584..2540
                     /locus_tag="VFL49_13210"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET7912986.1"
                     /translation="MRSYLDFEKPVAELEAKVEELRAMGESGDAVAISEEISRLETKA
                     ALALKELYADLTPWQKTQVARHPQRPHCADYVSGLITGFIPFAGDRKFGEDSAIVGGF
                     GRFRGESICVIGHEKGADTETRIKHNFGMARPEGYRKAVRLMEMADRFGIPVISLVDT
                     AGAYPGIGAEERGQAEAIARSTDACLSLGVPNIAVIVGEGGSGGAIAIATANRVLMLE
                     HAIYGVISPEGAASILWRDTTKAQEAATSMKITAQDMVRFGVIDSVVPEPTGGAHRDP
                     QGAIAVTGEAIAGALSELQGLDRDAVRKQRRDKFLAMGRAAV"
BASE COUNT          509 a          834 c          797 g          476 t
ORIGIN      
        1 ggcccggaaa taaggctcgc catgtttctc gaaaatttcc ggaatggtca tgcccgccgc
       61 actttcaatt tcgctatcgg catcgacgaa gggaattccc agacggccgg caagtctgcg
      121 gccgatcgac gatttgccgg cccccatcat acccacaagc acgattgagc ggccgccaag
      181 cgcgcggacc actgtcgaat cgggcccgtt ttccgccggt ttttggatgg cgccgttaac
      241 catgctagtc tttcgaacga atcctcggtt ccatatatca gcaaccgcgt ggccggtctt
      301 gccagcggtt cgggaacgtg gtcgtaactc gccgcagatc gccgcggtcc ggcctcgacg
      361 gaaacagtca tgccgagtct gttcagattt ctggtgcttt ttggcctgct tggcggcctg
      421 gcttacggcg cggttttctc gctcgcgacc ttcgtcaagt acaagccgcg tgagatcatc
      481 gtgaccattc cgcccgacaa gttcgtcaag aaccactgac gccggaaacg aggcaaggcg
      541 atgagccgcc ggccaggggc atccgacgaa gcgctgatcg aactgtttct cgacatgctc
      601 gccgccgaac gaggcgcaag cgagaacacg ctgaccgcgt atcgcaacga tctcgaggat
      661 ctgtccgcac atctgcgcgc ttcgcggagc acgatagcaa acgcgaccac ggacaatttg
      721 cgcaagttca ttgcaagcct cgccgagcgc ggcttcaagc cgtcgtcgct ggcgcggcgg
      781 ctttcggccg tgcgccagct ttatcgtttc ctctatgcgg aggggaagcg cggcgacgat
      841 cccgcggccg ttctcgaagg gccaaagcgc gggcgttcgt tgccgaaggt gctttcgatc
      901 gccgatgtcg acacgctgct caagaccgcg cggaccagcg gcgacaatgt cggacagccg
      961 ctccttcagc gtctgcgtgc cgcgcgcctt ctgtgccttc tggaagtcgt ctacgcgacc
     1021 ggcttgcgcg tgtccgaact ggtggcgctt ccggccgccg ccgcacgccg cgatcagcgc
     1081 atgctggtgg tgcgcggcaa aggcggcaaa gaacgtctgg tgccactcaa caagcaggcc
     1141 aaacgcgcca tggccgaata tcttgagctg cggagcaaag ccaagcgaga tgcggagtca
     1201 aaatggctat ttccgtcttt cggcgagcaa ggacacctga cgcgccagca cttcgcacgc
     1261 gagctcaaga cgctgggagc ggcctgcgga attcaaccgg cgcggctcag tcctcatgtc
     1321 ctccgacacg cttttgccag tcaccttctg cacaacggcg cggacctgcg cgtggtccag
     1381 accctgctcg gtcacgccga catttcgacg acccaaattt acactcacgt gctggaagag
     1441 cggctgaaaa aactggtgcg cgatcttcat ccgttggcgg acggctagcc aaaaacgtcc
     1501 gtaaaagcga aattaacccg cttgcttgac taagaggcat tccccgcgtc agttcgcccg
     1561 aaaagcacat ttgcccggac ccaatgcgca gttacctcga ttttgaaaag ccggtggccg
     1621 aactggaggc caaggtcgag gaattgcgcg ccatggggga gagcggcgac gcggtcgcca
     1681 tcagcgagga gatcagccgg ctcgaaacaa aggccgcgct cgctttgaag gagttgtacg
     1741 ccgacctcac gccctggcag aaaacgcagg tggcccgcca tccgcagcgt ccgcattgcg
     1801 ccgactacgt cagcgggctc attaccgggt tcatcccctt cgcgggcgac cgcaaattcg
     1861 gcgaggactc cgcgattgtt ggtggcttcg gacggttccg cggcgaaagt atctgtgtca
     1921 tcggtcacga aaaaggcgcc gacaccgaaa cccggatcaa gcacaatttc ggcatggcac
     1981 gtccggaagg ttatcgaaaa gccgtacgtc tgatggaaat ggccgatcgc ttcggcattc
     2041 cggtcatctc gctcgtcgac accgccggcg cctaccccgg tatcggtgcc gaggaacgtg
     2101 gccaggcgga agcgatcgca cgctcgaccg atgcctgtct gtcactcggc gtcccgaaca
     2161 tagcggtgat tgtcggcgaa ggcggctccg gcggtgccat cgccatcgcc acggccaatc
     2221 gcgtgctgat gctcgagcat gccatctacg gcgtgatctc gcccgaaggc gccgcttcga
     2281 tcctctggcg cgacacaacc aaggcgcagg aggcggcgac cagcatgaag atcaccgccc
     2341 aggacatggt gcgattcggc gtcatcgaca gcgtcgtccc cgagccaacc ggtggcgccc
     2401 accgggatcc acagggcgcc atcgcggtca ccggcgaggc catcgccggt gccctttcgg
     2461 aactgcaggg actcgaccgc gatgcagtgc gaaaacagcg gcgcgacaag tttctcgcta
     2521 tgggccgtgc cgccgtgtag gacggcgctg tttacttttg ctttaccatg gggttagcgg
     2581 tagcccggga cggcgcagat cgccttgcag atgcca
//
LOCUS       DASSRL010000646         5588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_51835, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000646 DASSRL010000000
VERSION     DASSRL010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51835"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /locus_tag="VFL49_13215"
     CDS             <1..1700
                     /locus_tag="VFL49_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596579.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HET7912987.1"
                     /translation="ATFIVECSEETWRRAGFDRMNTEETIAACEKIFARNLDGHKLMS
                     NARHLRGSAWLNFNRVLCERWSHKNIVLLGDAAATAHFSVGSGSKLALESAIALADYL
                     HSEPTMARAFRRYEDERRLEVLRLQSAARNSTEWFEDVERYLHLDPVQFNYSLLTRSQ
                     RISHENLRERDENWLGGAERWFERQATGREPQIQRPPMFVPFRVRGIELKNRVVVSPM
                     AQYRAVDGTPADWHLVHYAERAKGGAGLVFTEMTCVSPEGRITPGCTGLYRPEHEAAW
                     KRIVDFVHAESDAKFAIQLGHSGPKGSTQLGWETMDAPLPAGNWTVMAPSAVAWSPDN
                     QAPRAMTRADMDKVRDEFVRSAQMADRAGFDWLELHYAHGYLMSAFITPLTNKRTDEY
                     GGNLENRMRFPLEVFRAVRAVWPDDKPMSVRISANDWVPDGILPRDAVEVARILTDAG
                     ADMIDVSAGQTSRQAKPVYGRMFQTPFSDRIRNEAGIATLAVGNIYDPDHVNSILMAG
                     RADLVCLARPHLANPYWTLHAAADLGYEGLTWPKPYWAGRDQLHRLKSRPDVMTGKV"
     gene            1700..2467
                     /locus_tag="VFL49_13220"
     CDS             1700..2467
                     /locus_tag="VFL49_13220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7912988.1"
                     /translation="MGALDGLHALITGGGTGIGAAIARALAKEGAAVTLVGRRKAPLD
                     GIAKNLPKAVAIAADVTNATDCAAMVDAARAAHGPIDIIVANAGAAESAPAARISAEH
                     WQRMIGVNLTGAFLTVQAALSDVTRKSRGIRRIVFTASTAGLKGYPYVAAYCAAKHGV
                     VGLARALAAELKGDGLTVNAVCPGYTDTPLLDAAAAAVASKTGRSVDDARAALARDNA
                     DGRLISPDDVAAKVVWLCSPAANAVNGEAIVIAGGAA"
     gene            2464..2955
                     /locus_tag="VFL49_13225"
     CDS             2464..2955
                     /locus_tag="VFL49_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7912989.1"
                     /translation="MTIAVRSKARQTTPPLSKQRLRLWIRLLRAARAIEAEVRERLRV
                     QFDVTLPQFDVMAALERKPDGMTMTELSRFLMVSNGNVTGIIDRLVSDKLVVRQAPAD
                     DRRAIIVRLTPRGLKEFTAIAKAHESWVDALLSEFGAAEAGTLIDHLDGLSGRIRNGG
                     SRA"
     gene            2958..3761
                     /locus_tag="VFL49_13230"
     CDS             2958..3761
                     /locus_tag="VFL49_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET7912990.1"
                     /translation="MSDFRPRHFNWAAKDGVAVITLKGAERKNPITFESYAELGRTFR
                     ELSRAGDIKVVVFGSSGGNFCSGGDVHDIIGPLTSRDMQGLLEFTRMTGELVKAMRAC
                     PQPIIGAIDGVCVGAGAMIALFCDIRLGTPRAKTAFLFTRVGLAGCDMGACALLPRVV
                     GYGRASELLYTGRVMTAEEGERWGFFNRIVAADKLEADAMALAQQLAAGPTFAHGMTK
                     TMLHQEFAMPLDEAIEAEALAQAACMQTKDFRRAYEAFVAKQKPQFAGN"
     gene            3764..4927
                     /locus_tag="VFL49_13235"
     CDS             3764..4927
                     /locus_tag="VFL49_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7912991.1"
                     /translation="MLDRSFLRWPFFEERHRTLSDRLETWAAQHCTHVDHRDVDAACR
                     DLVRKLGRDGWLKHTAPGDDGPEVIDVRTLALIRETLARHSGLADFAFAMQGLGAGPV
                     SLFGNPSQKGRWLPKTRGGAAIAAFALSEPASGSDVANISTTARRDGDGFILDGEKTW
                     ISNGGIADLYVVFARTGEAAGAKGLSAFIVRGDNPGLSVAERIEVMAPHPLARLAFKD
                     CRVGADDLIGTPGDGFKIAMATLDVFRTSVGAAALGFARRALEETLKRAKTRELFGAP
                     LSELQMVQGHIADMAVEIDAAALLVYRAAWTKDTGAARVTREAAMAKLYATEAAQRVI
                     DAAVQIHGGDGVRSGHPVETLYREIRALRIYEGASDVQKIIIARSALSSAGGH"
     gene            4929..>5588
                     /locus_tag="VFL49_13240"
     CDS             4929..>5588
                     /locus_tag="VFL49_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7912992.1"
                     /translation="MTRSIKLGPSAHVDTFTRDNLPPFDQWPDIPLDRPEFQYPERLN
                     AAVELTDRMVERGFGDHIALIGNGRRRTYKELADWSNRLAHALVENYGVKPGNRVLIR
                     SGNNPALVAAWLAATKAGAVVVNTMPMLRAGELSKIIDKAEIGLALTDSRIADELVAC
                     AKASKFLKQVVNFDGTSNHDAELDRVALDKPVKFDAVATGRDDVALLGFTSGTTGEPK
                     AT"
BASE COUNT         1024 a         1690 c         1827 g         1047 t
ORIGIN      
        1 ccgccacctt catcgttgag tgttcggaag agacctggcg ccgggccggc ttcgaccgga
       61 tgaataccga ggagacgatt gcggcctgcg aaaagatctt tgctcgcaat ctcgacggcc
      121 acaaattgat gtcgaacgcg cgccatctgc gaggctcggc gtggctcaac ttcaaccgtg
      181 tcctgtgcga gcgctggagc cacaagaata tcgtcttgct cggagatgcc gcagcgaccg
      241 cacatttctc ggtcggctca ggttccaaac tcgcactgga aagcgccatt gctcttgccg
      301 attatctgca cagcgagccg accatggcgc gtgctttccg tcgctacgaa gacgaacggc
      361 ggctcgaagt cctgcgtttg cagagcgccg cgcgcaattc caccgaatgg tttgaggacg
      421 tcgaacgcta tctccacctc gatccggtcc agttcaacta ttcgctgctg acgcgttcgc
      481 agcgcatcag ccacgagaac ctgcgggagc gcgatgagaa ctggctcggc ggcgcggagc
      541 gctggttcga acgccaggcg acagggcggg agccgcagat ccagcgacct ccgatgttcg
      601 tgccgttccg cgtgcgcggc atcgagctga agaaccgggt cgtggtgtcg ccgatggcgc
      661 aatatcgtgc ggtcgacggc acgccggccg actggcatct ggtccattac gccgagcgcg
      721 ccaaaggcgg ggccgggctg gtttttaccg aaatgacctg tgtttccccg gagggccgta
      781 ttacgcccgg ctgcacgggc ctttatcgcc cggagcatga ggctgcgtgg aagaggatcg
      841 tcgatttcgt ccatgccgaa agcgacgcca agtttgccat acagctcggg cattccggtc
      901 cgaaaggctc gacccagctc ggctgggaaa cgatggatgc gccgctgccg gccggcaact
      961 ggacggtgat ggctccgtcg gcggtggcct ggagtccgga caatcaggcg ccgcgcgcga
     1021 tgacgcgtgc cgacatggac aaggtgcgcg acgaattcgt ccgctcggcg cagatggcgg
     1081 atcgcgccgg gttcgactgg ctcgaattgc actacgcgca cggctacctg atgtctgctt
     1141 tcatcacccc gctcaccaac aagcgcacgg atgaatacgg cggcaatctc gagaaccgca
     1201 tgcggtttcc gcttgaggtg tttcgtgcgg tgcgcgcggt gtggccggac gacaagccga
     1261 tgtcggtgcg catttccgcc aacgattggg tgccggacgg tatcttgccg cgggacgcag
     1321 tcgaggtcgc acgcatttta accgacgccg gcgccgacat gatcgacgtt tctgccggac
     1381 agacctccag gcaggccaag cccgtctatg gacggatgtt tcagacgccg ttctctgacc
     1441 gcatccgcaa tgaggcgggc atcgcgacac tcgcggtcgg gaacatctac gaccccgatc
     1501 acgtcaattc gatcctgatg gcagggcgcg ccgatctcgt atgtctggcg cgaccgcatc
     1561 tcgccaatcc atactggaca ctgcatgcgg cggccgacct cggctacgaa ggtctgacct
     1621 ggccgaagcc ctattgggct ggccgtgatc agctgcatcg cctgaagtcg cgccccgacg
     1681 tgatgacggg gaaagtgtga tgggcgcact cgacggtttg cacgcgttga ttacgggtgg
     1741 cggcaccggc atcggggcgg cgattgcgcg ggccctggcg aaagaaggtg ccgccgtgac
     1801 gcttgtcgga cggcggaagg cgcccctcga cgggattgca aagaatttgc cgaaagccgt
     1861 cgcgattgca gccgacgtga cgaatgcgac ggattgtgcg gcgatggtcg acgccgcgcg
     1921 tgccgcgcat ggaccaatcg atattattgt cgccaatgcg ggcgcagccg aaagtgcacc
     1981 ggcggcgagg atttcggccg aacattggca gcgcatgatc ggggtcaacc tgaccggcgc
     2041 gtttttgacg gttcaggcgg ctttgagtga cgtgacgcgc aagagcaggg gtatacggcg
     2101 gatcgtgttc accgcgtcga ccgctggatt aaagggctat ccctacgtgg cggcatactg
     2161 tgcggccaag cacggagttg tcggcctcgc gcgtgctctg gcggcggagc tcaagggcga
     2221 cggactcact gtgaacgccg tttgcccggg atacacggat acgccgcttc tggatgctgc
     2281 cgccgccgcg gttgccagca agacggggcg ctctgtcgac gatgcgcgcg ccgcgcttgc
     2341 cagggataac gcggatggcc gactgatttc accggacgat gtcgcggcca aggtcgtgtg
     2401 gttgtgttcg ccggccgcga atgcggtcaa cggcgaagcc attgtcattg ccggaggtgc
     2461 ggcatgacga tcgccgtgag atcgaaagca cgacagacaa cgccgccgct gagcaaacaa
     2521 agactgcggt tatggatccg cctgttgcgc gccgcacgcg cgatcgaagc cgaggtgcgc
     2581 gagcggctcc gagtccagtt cgacgtgacg cttccgcaat tcgatgtgat ggccgcactc
     2641 gaaaggaagc cagacggcat gactatgacg gagctgtcgc gcttcctgat ggtatcgaac
     2701 ggcaacgtca ctggcatcat cgaccggctg gtgtcggaca agcttgtcgt gcgccaggcc
     2761 ccggccgatg atcgccgggc tatcattgtg cggctgacgc cgcgcggctt aaaggaattc
     2821 accgcaatag ccaaagcgca cgagtcctgg gttgatgcct tgctatcgga attcggcgcg
     2881 gcggaagcag gcacattgat cgatcatctc gatggattgt ccggccgcat tcgcaacgga
     2941 ggctcgcgcg catgaatatg agcgacttca ggccgaggca ttttaactgg gcagcgaaag
     3001 atggcgttgc ggtgattacg ctcaagggcg ccgagcgtaa aaatccgatc accttcgaaa
     3061 gctacgccga actggggcgg actttccgcg agctttcccg ggccggcgat atcaaggtcg
     3121 tcgtgtttgg ctcgagcggc ggcaatttct gctcaggcgg cgatgtccac gacattatcg
     3181 ggccgctgac gagccgcgac atgcagggcc tgcttgaatt tacacggatg acgggcgagc
     3241 tcgtgaaggc gatgcgcgcc tgcccgcaac cgatcatcgg ggcaattgac ggcgtgtgtg
     3301 tcggggccgg cgcaatgatc gccttgttct gcgatatccg gctcggcacg ccgcgggcca
     3361 aaaccgcgtt cttgtttacc cgcgtcggcc ttgccggctg cgacatgggc gcgtgcgctt
     3421 tgctgccgcg tgtcgtcggc tatggccgcg cgtcggagct gctttatacc gggcgcgtca
     3481 tgacggcaga ggagggcgag cgttggggtt ttttcaatcg catcgttgcc gccgacaagc
     3541 tcgaagccga tgcgatggcg ctcgcgcaac aactggcggc gggcccgact ttcgcgcacg
     3601 gcatgaccaa gaccatgctg caccaggaat tcgcgatgcc gctcgatgaa gcgatcgagg
     3661 cggaggcgct cgcgcaggcg gcctgcatgc agacgaagga cttccgccga gcctacgagg
     3721 cttttgttgc caagcagaag ccgcagttcg ccggaaattg acgatgctcg accgtagttt
     3781 cctgcgttgg ccgtttttcg aagaacgtca tcgcacgctt tccgacaggc tggaaacatg
     3841 ggccgcgcag cattgtacgc atgtcgatca ccgcgatgtt gacgccgcct gccgtgatct
     3901 ggtccgaaag cttggtcgag acggctggct caagcacact gccccgggtg atgatggtcc
     3961 ggaggttatc gacgtccgaa cactggcttt gatccgtgag acgctggcac gtcattccgg
     4021 cctcgccgat tttgcgtttg cgatgcaagg ccttggcgcc ggcccagtct cgttgttcgg
     4081 taacccctct caaaagggcc ggtggctgcc caagactcgc ggcggtgctg ccatcgcggc
     4141 atttgcactt tccgagccgg catccggttc ggacgtcgcc aacatttcaa cgacggcgcg
     4201 gcgggacggc gacggcttca ttctcgatgg cgagaagacc tggatatcga acggggggat
     4261 cgccgacctc tatgtcgttt ttgcccgtac cggcgaggcg gcaggcgcga agggcctgtc
     4321 ggctttcatc gtgcgcggcg acaatcccgg cctgtcggtc gcagagcgca tcgaagtaat
     4381 ggcgccgcat ccgctcgcgc gccttgcttt caaggattgc cgtgtcggtg ctgacgatct
     4441 catcggaacg cccggcgatg gcttcaagat cgcgatggct accctcgacg tcttccgcac
     4501 atcggtgggt gccgccgcac tcggcttcgc gcgtcgtgca cttgaggaga cgctcaagcg
     4561 cgccaaaacc cgtgaactct tcggcgcccc attgtcggaa ttgcagatgg tacaaggcca
     4621 catcgccgac atggcggttg aaatcgacgc tgccgcgctg ctcgtctatc gtgccgcgtg
     4681 gaccaaggat acaggggcgg cgcgcgtaac gcgcgaagcg gcgatggcca agctttacgc
     4741 cactgaggcg gcgcagcgcg tgatcgacgc cgcggtgcag atccacggcg gcgatggcgt
     4801 gcgctccggg catccggtcg agacgctgta tcgtgaaatc cgcgccttgc gaatttacga
     4861 aggcgcgtcc gacgttcaga aaattatcat tgcacgatct gcgctttcgt cggccggagg
     4921 ccactgacat gactcgaagc atcaaacttg ggccgtccgc gcacgtcgat acctttaccc
     4981 gcgacaatct tccgccgttc gatcagtggc ccgatatccc gctcgaccgg ccggaatttc
     5041 agtaccccga gcgattgaac gctgcggtcg aactgacgga ccgcatggtt gagcgtgggt
     5101 ttggagacca cattgcgctg atcggcaatg gccgccggcg gacctacaag gaactggccg
     5161 actggtcgaa tcgactggcg catgcgctcg ttgagaacta cggggtcaag ccgggcaatc
     5221 gcgttttgat ccggtccggc aacaaccccg cgctcgtcgc ggcctggctc gccgccacca
     5281 aggccggagc ggtggtcgtc aatacgatgc cgatgcttcg cgccggagaa ctgagcaaga
     5341 taattgacaa agccgagatc gggctcgctt tgaccgacag ccgcattgcg gatgagctgg
     5401 tcgcctgcgc caaggccagc aaatttctca agcaagtcgt caattttgac ggcacatcca
     5461 atcacgacgc cgagctcgat cgcgttgcgc tcgataagcc ggtgaagttt gacgcggtag
     5521 ccacgggacg cgacgacgtc gcattgctcg gcttcacgtc cggtaccacc ggcgagccca
     5581 aggcgacg
//
LOCUS       DASSRL010000647         6588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_577035, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000647 DASSRL010000000
VERSION     DASSRL010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577035"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFL49_13245"
     CDS             complement(<1..185)
                     /locus_tag="VFL49_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET7912993.1"
                     /translation="MTERVTIARLGHRGDGIADTPGGMAYVPYTLPGENVTVERVTGH
                     PDRRHLLRVDKPSHERV"
     gene            complement(228..1646)
                     /locus_tag="VFL49_13250"
     CDS             complement(228..1646)
                     /locus_tag="VFL49_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7912994.1"
                     /translation="MKNPTNEATTAALPPNLWRGEIAETIRLALPIAFTQLGQVAMMT
                     SDLVLVGRLGDKAVAASALAQIILYSGFMLGMGLVSAVAPLAAQAFGAREPRMVRRAL
                     RVGLWVSLMLGVPLLAVQLHGERILLALGQEPGTARLAGQYLAGLAWSVVPGWWFIAV
                     RGFMGAVNRPEPALWITLAAIPINTLAAYALIFGAFGLPRLEMIGAGIATTLVYIGMC
                     VAAFWIAYTRPPFRKYHVLGRFWRADWSLLWRLVVVGWPISVSFLLEYGLFATAALMV
                     GWLGTTQLAAHQIALQVAAIVFMVPLGVSMAATVRVGHAVGRNDPASARRAGFSAVGL
                     GAIFMAAMVVLIIALRHVIPLAFLDRGTGDADATIALTENLLVIGATFFIVDGIQTVA
                     AGALRGLNDTRVPMIFAALSFWAVGFTACYLLGFPAQLGVIGVWIGFTICLLLYAFLL
                     TWRFDTLTRRGYMPVAAHTSQG"
     gene            complement(1739..2542)
                     /locus_tag="VFL49_13255"
     CDS             complement(1739..2542)
                     /locus_tag="VFL49_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET7912995.1"
                     /translation="MVPLSRIRKAQAATSQRQRADRLLVERGLFESRAKAQAAIEAGH
                     VFADDQPVRKASEELAADAVIRASAAHPYVSRGGVKLAAALDRFGLDPKGRVCLDVGS
                     STGGFTQVLLDRGARRIYAVDVGHGQLHESLRSQPPITSLEDTDIRALSPRLLDEAPD
                     VITVDVSFISLKLVLPAALALAKAPAWLVALIKPQFEAGPTVVKKGIVRDPAMRAEIC
                     DSLATFTAGLGWRVIGIMPSPIKGGEGNTEFLLAAERPPNVSSPQAMNR"
     gene            2670..2810
                     /locus_tag="VFL49_13260"
     CDS             2670..2810
                     /locus_tag="VFL49_13260"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET7912996.1"
                     /translation="MKKRKKGKLRSGRSGKKVKSRKQAIAIGLSEARKKGKKVPRRKK
                     KL"
     gene            complement(2814..4736)
                     /gene="dxs"
                     /locus_tag="VFL49_13265"
     CDS             complement(2814..4736)
                     /gene="dxs"
                     /locus_tag="VFL49_13265"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET7912997.1"
                     /translation="MSEVSKTPLLDRVNLPSDLKNLSPAQLRQLADELRHEMIDAVAR
                     TGGHLGAGLGVVELTVALHYVFDTPADRLIWDVGHQAYPHKILTGRRDRIRTLRQGGG
                     LSGFTKRAESEYDPFGAAHSSTSISAALGMAVARDFQGKKNNVIAVIGDGAMSAGMAY
                     EAMNNAGARNERLIVILNDNDMSIAPPVGAMSSYLSRLLSGRTYLKLRDVGKQLTAHL
                     PKRLDEFITRGVEHARGFWTGGTLFEELGFYYVGPIDGHNLDHLLPVLRNVRDAEAGP
                     ILVHVVTQKGKGYAPAESSADKYHGVVKFDVATGAQAKSKPGAPQYTKVFGESLIKEA
                     RKDDKIVAITAAMPSGTGLDLFAKEFPKRCFDVGIAEQHAVTFAGGLAAEGLKPFAAI
                     YSTFLQRAYDQVVHDIAIQRLPVRFAIDRAGLVGADGPTHAGSFDVAYLGCLPGFVLM
                     AAGDEAELVHMVATAAAIDDRPSAVRYPRGEGLGVPMPEEGRPLEIGKGRIVQEGHKV
                     ALLSYGGRLGECLAAARELKALGLSATVADARFAKPLDIELLTRLVRDHEVLVTVEEG
                     AIGGFGSYVLQALAEHGLIERGLRVRCMVLPDQFIDQDTPNAMYAQAGLDAKGIVAKV
                     FEALGKDVAAETVRLA"
     gene            complement(4853..5101)
                     /locus_tag="VFL49_13270"
     CDS             complement(4853..5101)
                     /locus_tag="VFL49_13270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7912998.1"
                     /translation="MAENNVDVKKMPFERAIEELESIVKRLEEGKVPLEESVAIYERG
                     EALKTRCEELLRQAEARVEKITLNTSGKPTGSEPLDVD"
     gene            complement(5156..6085)
                     /locus_tag="VFL49_13275"
     CDS             complement(5156..6085)
                     /locus_tag="VFL49_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET7912999.1"
                     /translation="MSTLLLSHPACLNHLTPAGHPERPDRLRAIEQVLEDEKFQLLAR
                     VEAPRGPLDIVALCHPAEYIEAIRDASPAEGLVRLDADTAMSPGSFEAALRAVGGAKL
                     AVDEVIAGKASNAFVATRPPGHHTETARPMGFCLFNNAAIAARYAQKTHGIERAAIVD
                     FDVHHGNGSQDIFWADKSVMYCSTHEMPLYPGTGAVGERGEFNTIVNAPLSAGDGGGA
                     FKEAFETVILPRLREFKPDILIISAGFDAHTRDPLANLNLVEEDYTWVTKKLMDVADA
                     SAKGRVVSLLEGGYDLQGLSRSVAAHVTALMHG"
     gene            complement(6125..>6588)
                     /locus_tag="VFL49_13280"
     CDS             complement(6125..>6588)
                     /locus_tag="VFL49_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HET7913000.1"
                     /translation="LVRAANPHILMLSPPLDMSEKWFAPHTAYSMAKFGMSLVVLGLA
                     GELRGKVAVNALWPRTTIATAAIRNLLGGDTMMRRSRKPEILAEAACRIFEKPKTFTG
                     NFLIDDTFLAGEGVTDFDRYRVDPSQPLQIDFFVPDSSRPPSGVSLDAKSR"
BASE COUNT         1296 a         2031 c         2105 g         1156 t
ORIGIN      
        1 tgcacgcgct cgtggctggg cttgtcgaca cgcagcagat gtcgccggtc gggatggccg
       61 gtaacgcgtt cgacagtgac gttttccccg ggaagcgtat agggcacata ggccatgccg
      121 ccgggagtgt cagcgatacc gtcaccgcgg tggccaagcc gggcaatggt gacgcgttcg
      181 gtcatgccct gccttagccg accggcggcc ttgttcgcaa cgcgccttca gccctgcgac
      241 gtgtgcgcgg cgaccggcat gtaaccgcgg cgcgtaagag tatcgaagcg ccaggtcagc
      301 aggaaagcgt acagcaggag acaaatggta aatccgatcc atacgccgat cacgccgagt
      361 tgcgccggga agcccagcaa atagcaagcc gtaaacccga ctgcccagaa actcagcgcc
      421 gcaaagatca tgggcacgcg ggtatcgttg agaccacgca atgcgccggc cgcgacggtc
      481 tggatgccgt ccacaataaa gaacgttgca ccgatgacga gcagattttc cgtcagcgcg
      541 atggtcgcgt cggcgtcacc ggttccgcga tcgagaaacg cgagcggaat gacgtgtcgt
      601 agcgcgatga tgagaacaac cattgccgcc ataaaaatgg caccaagacc gaccgccgaa
      661 aatccggcgc gtcgcgccga cgccgggtcg ttgcgcccga ccgcatggcc gacgcgcaca
      721 gtcgccgcca tggaaacgcc aagcggcacc atgaagacga tggcggcgac ctgcagtgcg
      781 atctgatggg cggccagttg tgtcgtgccg agccatccga ccatgagagc ggcggtcgcg
      841 aaaagaccgt attcaagcag aaaggaaacc gagattggcc atcctacgac cacgagccgc
      901 cagagcagag accagtctgc gcgccaaaac cggccgagca cgtggtattt cctgaacggc
      961 ggccgggtgt aggcgatcca gaatgccgcg acgcacattc cgatatagac cagcgtcgtc
     1021 gcgatccctg cgccgatcat ctcaagacgg ggtaatccga acgcgccgaa aatgagcgca
     1081 taagccgcca gggtattgat cgggatagcg gcaagtgtga tccacagcgc gggctctggc
     1141 cggttcaccg cgcccatgaa gccgcgcacg gcgatgaacc accatcccgg cacgacactc
     1201 caggcgagtc cggcgagata ttgcccggcg aggcgcgcag tgccgggctc ctgtccgagc
     1261 gcgagcaaaa tgcgctcgcc gtggagctga acggcgagaa gcggcacgcc cagcatcagc
     1321 gaaacccaga gtccgacgcg cagcgcgcgg cgcaccatgc gcggctcgcg cgcaccgaag
     1381 gcctgcgcgg ccagcggggc gacagcggaa accaggccca tgcccagcat gaagccgctg
     1441 taaaggatga tttgcgccaa ggccgacgca gcgacggctt tatcgccgag ccggcccacc
     1501 agcaccagat cgcttgtcat cattgcgacc tggccgagct gcgtgaatgc gattggcagc
     1561 gcgagacgga ttgtttccgc gatctcgccg cgccagaggt tcgggggcag cgcggcagtc
     1621 gtcgcttcgt ttgttggatt tttcaccgcg cgcaccgtac agcgcggtgt ggcgagacta
     1681 aggcttgcaa atcaccgatt tcttcaacag acggcgcggc gagacgaatc aaaatcgttc
     1741 atctgttcat cgcctgcggt gatgaaacgt tcggcggccg ctcggccgcc agcagaaatt
     1801 ccgtattccc ctcgccacct ttgatgggcg agggcataat tccgatcacg cgccagccga
     1861 gccctgccgt aaaggtggcg aggctgtcac aaatctcggc gcgcatggcc ggatcacgga
     1921 caatcccttt cttcacgaca gtggggccgg cctcgaactg tggcttgatc agcgcgacaa
     1981 gccaggcggg cgctttggcg agcgcgagcg ccgccggcag gacgagcttg agcgatatga
     2041 agctgacgtc gacggtgatg acatccggcg cttcatcgag caggcgaggg gagagcgcgc
     2101 ggatgtcggt gtcttcaagc gacgtaatcg ggggctgcga tcgcagcgac tcatgcaatt
     2161 gaccatggcc gacgtcgacg gcgtaaatgc gcctcgcacc gcgatcgagc agcacctgcg
     2221 taaatccgcc ggtggacgag ccgacgtcga gacagacacg ccccttcgga tcaaggccga
     2281 aacggtcgag cgcggctgca agcttgacgc cgcctcgcga gacatacggg tgggcggcgc
     2341 tcgcgcgaat gacggcatcg gccgccagtt cttctgacgc cttacgcacc ggctgatcgt
     2401 cagcgaacac atgcccggct tcgatcgccg cctgggcctt ggcgcggctt tcaaacagtc
     2461 cgcgctcgac aagaaggcga tcggcgcgtt ggcgttgtga ggtcgcggcc tgcgcctttc
     2521 gaatccgcga gagaggaacc atgttctatc cacggcggtt agcaattttg ggccgcgagc
     2581 gtttgtcgcg gccaccatgg gttagctcaa ggagaaacag tatggcacgt aaatattcaa
     2641 gaagcgcttc aagggatgtc gaacgcgcga tgaagaaacg caagaaaggc aagctgagaa
     2701 gtggccgcag cggcaaaaag gtcaagagcc gaaaacaggc aatagcgatc ggcctgtccg
     2761 aggcgcggaa gaaaggaaag aaggtgccgc gaaggaagaa gaaactctaa agcctacgca
     2821 agtcgcaccg tttccgccgc aacatccttg ccgagcgctt cgaacacttt cgcaacgata
     2881 ccttttgcat cgagcccggc ctgggcatac atcgcgttgg gcgtgtcctg gtcgatgaac
     2941 tgatcgggca gcaccatgca acggacgcgc aggccgcgtt cgatcaagcc gtgctcggcc
     3001 agggcctgca ggacgtagga gccgaagccg ccgatggcgc cttcctcgac cgtcaccagc
     3061 acctcgtggt cgcgaaccag gcgagtcagc agctcgatgt cgagcggctt ggcaaagcgg
     3121 gcatccgcga ccgtggccga caggccgaga gctttgagct ccctggccgc ggccaggcat
     3181 tcgccgagcc ggccgccata cgacagaagc gccactttgt ggccttcctg cacaatgcgg
     3241 cctttgccga tctcgagcgg cctcccttct tcgggcatcg gtacgccgag accttcgccg
     3301 cgaggatagc ggactgcgga aggacgatcg tcgatcgccg ctgcggtcgc gaccatgtgc
     3361 acgagctcag cctcgtcgcc ggctgccatc agcacgaagc cgggtaggca gccgagatag
     3421 gcgacatcga aagaaccggc gtgggtcggc ccgtccgctc cgacgagccc ggcgcgatcg
     3481 atcgcaaatc gcacgggcaa tctctgaatc gcgatatcgt gcacgacctg atcgtaggcg
     3541 cgctgcagga aggtcgagta gatcgcggcg aagggcttga gcccttccgc ggcaaggcca
     3601 cccgcgaaag tgaccgcatg ctgttccgca atgccgacgt cgaagcagcg cttcggaaat
     3661 tctttcgcaa aaaggtcaag gcccgtgccc gaaggcatcg cggccgtgat cgcgacgatc
     3721 ttgtcgtctt tgcgggcttc tttgatcagg ctctcaccga aaactttcgt gtactgcggt
     3781 gcgccgggct ttgacttcgc ctgcgccccc gtcgcgacgt cgaacttcac cacgccgtga
     3841 tacttgtcgg cggacgattc cgccggcgca taacccttgc ccttctgcgt cacgacgtgc
     3901 acgaggattg ggcctgcttc ggcatcgcgc acgttgcgca gcacagggag cagatggtcg
     3961 agattatggc cgtcgatcgg gcccacatag tagaagccga gttcctcgaa cagcgttccg
     4021 ccagtccaga atccgcgcgc atgctcgacg ccgcgcgtga tgaattcatc gagccgtttc
     4081 ggcaggtggg cggtgagctg cttgccgacg tcgcgcaatt tcaaatacgt gcgtccggac
     4141 aggagccgcg aaagatagga cgacatcgcg cccaccggcg gcgcaatcga catgtcgttg
     4201 tcattgagga tgacgatcag ccgctcgttg cgcgcgccgg cattgttcat cgcctcgtag
     4261 gccatgccgg cggacattgc gccgtcgccg atcacggcga tgacgttgtt tttcttgccc
     4321 tggaagtcgc gggcaaccgc catgccaagc gcggccgaaa tcgacgtcga ggaatgcgcg
     4381 gcgccgaacg ggtcgtattc gctttcggcg cgcttggtga aacccgacag gccgccgcct
     4441 tgccgcagcg tgcggatgcg gtcgcgcctt ccggtgagga tcttgtgcgg ataggcctgg
     4501 tggccgacgt cccagatcag cctgtcggcg ggcgtatcaa agacataatg cagggcgacc
     4561 gtcagctcga cgacgccaag ccccgcgccg agatggccgc cggtcctggc aacggcgtcg
     4621 atcatctcgt ggcgcagttc gtcggcgagc tgcctgagct gcgcgggaga caaattcttg
     4681 agatcgctgg gcaggttgac gcgatcgagc aaaggcgtct tggacacttc ggacacgcac
     4741 aactccagtt gattggtcga cggcggaaaa gcggtaaaat cccccagatt cagccgcctg
     4801 acttacacta tttctgcctt gaggggcaat tcggcctctg gcccacgccg cttcagtcga
     4861 cgtcgagcgg ctcgctgccg gtcggcttcc cgctggtgtt taaggtaatt ttttcgaccc
     4921 gcgcctcggc ctggcgcagt aattcctcgc agcgcgtctt caatgcctcg ccgcgctcat
     4981 aaatggcaac ggattcctcg agcggcacct tcccttcttc caggcgcttg acgatcgatt
     5041 ccagctcctc gatcgcgcgc tcgaagggca ttttcttaac gtcgacgttg ttctcggcca
     5101 tatctctgtc cggtgcttct ttagcgtgca atgtagtgac gatagcgagg attctctatc
     5161 cgtgcatcaa tgcggtgacg tgggcggcaa ccgagcgcga aagcccctgc aaatcatagc
     5221 cgccctctaa taaagacacg acgcggcctt tggcgctcgc atcggcgacg tccatgagct
     5281 tcttggtgac ccacgtatag tcctcttcca ccaggttgag gttggcgagt ggatcgcgcg
     5341 tgtgcgcgtc gaatcccgcc gagatgatca ggatgtccgg cttgaattcg cgcaggcgcg
     5401 ggagaatgac ggtttcgaac gcttccttga aggcgccgcc gccgtcaccg gccgacaacg
     5461 gcgcgttgac gatggtattg aattcgccgc gttcgccgac ggcgccggtg ccggggtaaa
     5521 gcggcatctc gtgggtggag caatacatca cgcttttgtc ggcccagaag atgtcctgcg
     5581 agccgttgcc gtgatggacg tcgaaatcga caatcgccgc tcgctcgata ccgtgcgttt
     5641 tttgcgcata gcgcgccgcg atcgccgcat tgttgaagag gcagaagccc atcggacgtg
     5701 ccgtctcggt gtggtgtccg ggcgggcgcg ttgcaacgaa ggcgtttgac gctttgccgg
     5761 caatcacttc atcgaccgca agcttcgccc cgccgacggc gcgcaaggcc gcttcgaagc
     5821 tgcccggcga catcgcggta tcggcgtcca ggcgcacaag gccctcggcc ggcgaagcgt
     5881 cgcggattgc ttcgatgtat tcggccgggt ggcagagcgc gacaatatcg agcggcccgc
     5941 gcggcgcctc cacgcgcgcc agcaactgaa atttttcatc ctccaatacc tgctcgatcg
     6001 cgcgcagccg gtccggtcgc tccgggtggc cggcgggcgt gaggtggttc aggcaggcag
     6061 gatgactgag aagaagtgtc gacattgcct agacttaaac gctcatatgc ccgggtggaa
     6121 gggtttatcg cgattttgcg tccaggctga caccgctggg aggccggctg ctgtcgggca
     6181 caaaaaaatc gatctgcaga ggctggctcg gatcgacgcg ataacggtcg aaatcggtca
     6241 cgccttcccc ggcaagaaaa gtgtcgtcga tgagaaagtt cccggtgaaa gtcttcggct
     6301 tttcgaagat gcggcacgcg gcttcggcga ggatctccgg tttgcgcgag cggcgcatca
     6361 tggtgtctcc gccaagcaga ttccggatcg cggcggtcgc gatcgttgta cgcggccaga
     6421 gcgcgttgac cgcgaccttg ccgcgcaatt cgccggcgag cccaagcacc accaggctca
     6481 tcccgaactt tgccatcgaa taggcggtgt gcggcgcgaa ccatttttcg gacatgtcga
     6541 gcggcggcga cagcatcagg atgtgcgggt tcgcagcgcg cacgagat
//
LOCUS       DASSRL010000648         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1294244, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000648 DASSRL010000000
VERSION     DASSRL010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294244"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="flgA"
                     /locus_tag="VFL49_13285"
     CDS             complement(<1..538)
                     /gene="flgA"
                     /locus_tag="VFL49_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="required for the assembly of the flagellar basal
                     body P-ring; Bradyrhizobium has one thick flagellum and
                     several thin flagella; the Bradyrhizobium protein in this
                     cluster is associated with the thick flagellum; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HET7913001.1"
                     /translation="MTRLFLALFVAFGAFATAAGAQTPKELAPTRPTLKSEAVINGDI
                     VRIGDLIDNAGVVASVPIFRAPDLGHTGTVSADAVLEAVRDHALIGINTAGLREVVVT
                     RASRAIPEKDVEEVIARTLSTRFDLGAAKDIVVNFARDMHTIYVEPSALGQLKVTHID
                     YDMRGGRFDATLEIPVSAG"
     gene            complement(548..1336)
                     /gene="flgG"
                     /locus_tag="VFL49_13290"
     CDS             complement(548..1336)
                     /gene="flgG"
                     /locus_tag="VFL49_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HET7913002.1"
                     /translation="MRALHTAATGMMAQELNVQVISNNIANMRTTGYKRQRAEFQDLL
                     YEYVSRVGTQTSAQGNILPAGIELGSGVKTVGTPRLMSQGELTQTGKDFDLAIRGEGF
                     FKILLPDGRTSYTRDGSLELDAQGRLVTAQGNLVQPGITIPQNAMSVSVNAQGQVFVT
                     QPGSNTPSQVGQIELAIFVNKAGLQSIGDNMYLETPASGTPQNGNPNTEGFGNLQQGF
                     LEQANVEAVSEISDLIAAQRAYEMNAKVISAADQMLSSAANMMR"
     gene            complement(1354..2124)
                     /gene="flgF"
                     /locus_tag="VFL49_13295"
     CDS             complement(1354..2124)
                     /gene="flgF"
                     /locus_tag="VFL49_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HET7913003.1"
                     /translation="MQNALLVGLSRQVALGRELDVIANNIANLNTSGFKSDGAVFEEF
                     VSPTARADNFPAADRPISFVHDRATWIDLSQGPLERTGNSLDVAINGKGFLVVQTPRG
                     ERYTRNGAMQINSSGELVTAEGYQVVGESGPIVFQPKDRNITISEDGTISVREGNNAQ
                     TESQRGQLRVVSFDQPGRLQKDGAGVFLAPAGLTPQADKLSRVMQGTVEKSNVRSVME
                     MTRMIEVTRSYTQIANILQQQSELRRTAIEKLAEMPAN"
     gene            2486..3028
                     /locus_tag="VFL49_13300"
     CDS             2486..3028
                     /locus_tag="VFL49_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HET7913004.1"
                     /translation="MAAKAKEAEVEEDEKPEEENEQPASKSKLFKFIPKFKLPSLKWM
                     IIGGAGLLVFIGGGGGAYYFLKNSGHKEPQVAAKPATFVDLPDVLVNLSTSGGDRTQY
                     LKVKIVLELPDSSLVPQIQPLMPRVMDAFQTYLRELRPTDLDGSAGLYRLKEELTRRV
                     NAAVAPNHITAVLFKEIVVQ"
     gene            3067..>3347
                     /gene="fliM"
                     /locus_tag="VFL49_13305"
     CDS             3067..>3347
                     /gene="fliM"
                     /locus_tag="VFL49_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with FliG and FliN makes up the switch complex
                     which is involved in switching the direction of the
                     flagella rotation; Bradyrhizobium has one thick flagellum
                     and several thin flagella; the protein in this cluster is
                     associated with the thick flagellum; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HET7913005.1"
                     /translation="MATKDQIDQDALAAEWGLALESDTGAAPAGADPATAPPADDAAA
                     QWAAMADDPGGLQSAKGGAERILNQEEIDSLLGFSLADVSLNDNSGIRA"
BASE COUNT          650 a          980 c         1025 g          692 t
ORIGIN      
        1 ggccggcgct cacgggaatc tcgagtgtcg cgtcaaagcg cccaccgcgc atgtcgtagt
       61 cgatatgcgt gaccttcaac tgacctagcg cggaaggctc gacatagatt gtgtgcatgt
      121 cgcgcgcaaa attgacgacg atgtccttgg ccgctccgag gtcaaatctc gtggacaagg
      181 tccgggcgat cacctcttcg acgtcttttt cgggaatggc gcggctcgcg cgtgtgacca
      241 cgacttcgcg caggcccgcc gtattgatgc cgatgagtgc atgatcgcgt acggcttcga
      301 gtacggcgtc cgccgaaacc gttccggtat ggccgaggtc cggtgcgcga aagattggca
      361 ccgaggcaac gacgcccgca ttgtcgatca agtcgccgat gcgcacgata tctccgttta
      421 tgacggcttc cgacttcagc gtcggccggg ttggggcaag ctctttcgga gtttgcgcgc
      481 cggcagcggt tgcgaaagcg ccgaacgcga caaatagggc aaggaataga cgcgtcatgg
      541 cgacacctca gcgcatcatg ttggcggccg acgacagcat ctggtcggct gcggagatca
      601 cttttgcatt catttcgtag gcacgttgcg ccgcgatcag atcggaaatt tcgctgaccg
      661 cttcgacatt tgcctgctcg aggaagccct gctgcagatt gccgaacccc tcggtattcg
      721 gattgccgtt ctgcggcgtg cccgaggccg gagtctcgag atacatattg tcgccgatac
      781 tctggaggcc ggctttgttg acaaagatcg caagctcgat ctgtccgacc tggctcggcg
      841 tattggagcc cggctgcgtc acgaacacct ggccctgcgc attaacggat acagacatcg
      901 cgttttgtgg aatcgtgatg cctggctgca ccaggttgcc ttgcgcggtt accagtcggc
      961 cctgagcgtc aagttcgagc gaaccgtcgc gcgtatagga agtccggccg tcgggcagca
     1021 aaatcttgaa gaagccttcg ccgcgaatgg caaggtcgaa gtccttgccc gtctgggtca
     1081 gttcgccttg gctcatcaag cgtggcgtgc cgaccgtctt gacaccggat ccaagctcga
     1141 tgcctgcggg taagatgttg ccttgcgccg aggtctgcgt accgacgcgg ctgacgtatt
     1201 cgtagagcag atcctggaat tcggcgcgct ggcgtttgta gccggtggtc cgcatgttgg
     1261 cgatgttgtt ggagatgacc tggacgttta attcctgggc catcatcccg gtcgccgcgg
     1321 tgtggagcgc tcgcatagac tattctccct cggttagttg gccggcattt cggcgagctt
     1381 ctcaatcgcc gtacgtcgca attcggattg ctgctgaagg atgttggcaa tctgcgtata
     1441 gctgcgcgtg acttcgatca tgcgggtcat ttccatcaca gagcgtacgt tcgatttttc
     1501 gaccgttccc tgcatcacgc gcgacagctt gtcggcctgc ggcgtcagtc ccgccggggc
     1561 taggaagaca ccggccccgt ctttctgcag cctgccgggc tggtcgaaac tgacgacgcg
     1621 caattggccg cgctgcgatt ctgtttgggc gttattgcct tcacggacgc tgatggtgcc
     1681 gtcttcgctg atcgtgatgt tgcgatcctt gggctggaag acgatcgggc cggactcgcc
     1741 aacgacctga tagccctcgg ccgtcaccaa ctcgccgctg ctgttgatct gcatcgcccc
     1801 attgcgagtg taacgttcgc cgcgcggcgt ttgcacgacg aggaagcctt tgccattgat
     1861 ggcaacgtca agcgaattgc cggtacgctc gagcgggccc tggctcagat caatccaggt
     1921 cgcgcggtcg tgcacgaaac ttatagggcg gtcggcggcc ggaaagttat cggcacgcgc
     1981 agtcgggctg acgaactcct cgaacaccgc tccgtcgctt ttaaagcccg aggtgttgag
     2041 gttcgcgata ttattggcaa tgacgtcaag ttcgcggcca agcgcgacct ggcgtgacaa
     2101 cccgaccaga agtgcattct gcatcggcaa ctccccttgt aggtcggtcg ggtcggccct
     2161 cccgaggccg gcatcgaccg ctttcgatgc tgcaagctct cccaagcctg cagctgaccg
     2221 atgtcttgca gccgccatgc caacagaaaa atcattgtta gatcagaggc ttggcttaag
     2281 gataaagttg ttctggcgcg gcaaaaaccg accgtttgag gggtctaccg ggcaaaagtt
     2341 tcccgctggt caggtggagg aaagtatctg ttaaccatac ggacctacct tcgatcgcaa
     2401 ggggcgcgag cgcgcgcagc cgcggtgttt cgccaaaaac aacgtcgctt gccgtcccgc
     2461 tcaaactcgc gatccggggt cattcatggc agccaaggcg aaagaggcag aggtcgaaga
     2521 agacgagaag ccggaagaag aaaacgagca gccggcatca aaatcaaaac tcttcaagtt
     2581 catcccgaaa ttcaagctgc catcgctgaa atggatgatc atcggcgggg ccgggctttt
     2641 ggtctttatc ggaggcggag gcggcgccta ctatttcttg aagaactccg gccataaaga
     2701 gccgcaggtc gcagcaaagc cggcgacctt cgttgatctg cccgacgtcc tggtgaacct
     2761 ttccacttcc ggcggcgatc gcacgcagta tctcaaggtt aagatcgtgc ttgagcttcc
     2821 ggactcgagc ctggtgccgc agatccagcc gctgatgccg cgggtcatgg acgcattcca
     2881 gacctatttg cgggagttgc ggccgaccga tcttgatggc tcggcaggac tgtatcgtct
     2941 caaggaagaa ctgacacgcc gcgtcaatgc ggcagtcgcg ccgaaccaca tcacggccgt
     3001 gctgttcaag gaaattgtcg tccagtgaac gtgcgaagcg aataaacgtc agacgggctc
     3061 gggaccatgg cgacaaaaga ccagatcgac caagacgcgc tcgcggcgga atgggggctc
     3121 gcgctggaat ccgatacggg agccgctccg gcaggcgccg accccgcgac tgcgccgccg
     3181 gccgatgatg cggcggcgca atgggccgcg atggccgacg accccggcgg acttcaatcc
     3241 gccaaaggcg gtgctgagcg cattctcaat caggaggaaa tcgacagcct gctcggtttc
     3301 agtctcgccg acgtttcgct caacgacaac tccggtatcc gcgccat
//
LOCUS       DASSRL010000649         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1084537, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000649 DASSRL010000000
VERSION     DASSRL010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084537"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1511)
                     /gene="gltX"
                     /locus_tag="VFL49_13310"
     CDS             complement(87..1511)
                     /gene="gltX"
                     /locus_tag="VFL49_13310"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7913006.1"
                     /translation="MSEPVVTRFAPSPTGFLHIGGGRTALFNWLYARRHGGKMLLRIE
                     DTDRERSTAAAINAIIDGLTWLGLAWDGDIIFQFPRAARHRQVAEQLLASGHAYRCYC
                     SHDELAAMRERARAEGRTRLYDGRCRDRDAKDAPSGVSPVIRLKAPLTGETVVEDQVQ
                     GRVVWQNENLDDFVLLRSDGNPTYMLAVVVDDHDMGVTHIIRGDDHLNNAARQTQIYQ
                     ALGWKVPVMAHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAAMRNYLVRLGWAHGD
                     QEIFTTEEMIGAFDLPQIGRSPARFDFTKLESLNGHYIRATDDAALMAALEQLLPHIA
                     GGQELASKMTPALRAKFLAAMPALKERAKTLVELFEASRFLWTNRPIDINDQAKALLT
                     DEAKTLIKALLPELEAVSEWNAGSAEAVVRPYAERAGLKLGAIAQPLRAALTGRTTSP
                     PIFDVLAVLGRDESLARLRDQAAS"
     gene            1621..>2381
                     /locus_tag="VFL49_13315"
     CDS             1621..>2381
                     /locus_tag="VFL49_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET7913007.1"
                     /translation="MATDAKARGEPDLTAPGRDFIRDIVKGDLDAGRVEQVITRFPPE
                     PNGYLHIGHAKSICLNFGIAEEFGGHCHLRFDDTNPAKEEQEYIDAIEHDVRWLGFDW
                     GKHLYHASDYFEQLYAWAEDLIRAGKAYVDDQTPEQMRINRGTLTEPGKNSPFREREV
                     DENLDLFRRMRAGEFPNGARVLRAKIDMSSGNINLRDPVLYRILHAPHPRTGTAWKIY
                     PSYDFAHGQSDAIESITHSICTLEFEDHRPLYDWF"
BASE COUNT          485 a          716 c          724 g          456 t
ORIGIN      
        1 cttattcaag tgtgcactgc aagatagccc ggcgctgccg acatgccagg catataggcg
       61 ctcagaagcc gtttgcccgg tatgaactat gacgcagcct gatcacgtag ccgggcaaga
      121 ctttcgtcgc ggccgagaac cgccagtacg tcgaagatcg gcggagatgt ggtgcgcccg
      181 gtgagggccg cgcgcagcgg ctgcgcgatt gcaccgagct tgagccctgc gcgctcggcg
      241 tagggccgta caacagcctc ggcagagcct gcgttccact ccgaaacggc ttccagctcg
      301 ggaaggaggg ccttgatcaa cgtctttgcc tcgtcggtca ggagtgcttt cgcctgatca
      361 ttaatatcga tagggcggtt cgtccaaagg aaccgcgacg cttcgaacaa ttcgaccaaa
      421 gtcttggcgc gctccttcaa tgctggcatg gcagccagaa atttggcgcg tagcgccggt
      481 gtcattttgc tcgcgagttc ctgcccgccc gcaatgtgcg gcagaagctg ctcaagggca
      541 gccataaggg ccgcatcgtc ggtcgctcgg atataatggc cgttgagact ttcgagtttc
      601 gtgaagtcaa agcgcgcggg cgaccgccct atctgcggaa gatcgaaagc gccaatcatc
      661 tcttctgtgg tgaaaatttc ctgatcgccg tgcgcccagc ccagccgtac aagatagttt
      721 ctcatcgcgg ccggcagata ccccatggcc cgatatgcat cgacgccaag ggcgccatgt
      781 cttttcgaaa gcttggaacc gtccggtcca tgaatcaggg gaatgtgtgc catgaccggc
      841 accttccaac cgagcgcctg gtagatttgc gtctgccgcg ctgcgttgtt gaggtggtcg
      901 tcaccgcgga tgatgtgagt gacgcccata tcgtgatcat cgaccacgac cgcgagcatg
      961 taggttggat tgccgtcgga tcgcaggaga acaaagtcgt cgaggttctc attctgccag
     1021 acaacgcggc cctgaacctg gtcttcgaca accgtctctc ccgtcaacgg cgccttgagg
     1081 cggatgaccg gactgacacc ggacggcgcg tcctttgcat cgcggtcacg gcaacgtccg
     1141 tcgtagaggc gggtacggcc ttcggcgcgc gcccgctcgc gcatcgcggc aagctcgtcg
     1201 tggctgcaat aacaacgata ggcatggccc gacgcaagca gttgctccgc cacctggcga
     1261 tggcgcgccg cacgaggaaa ctggaagatg atgtccccgt cccaggccag tccgagccag
     1321 gtcaggccgt cgatgatggc atttatcgcg gccgccgtgg agcgctcccg gtctgtatcc
     1381 tcgatccgca acagcatttt gccgccgtgc cggcgggcat agagccagtt gaacaaagcc
     1441 gtgcgcccgc caccgatatg gagaaaaccg gtaggcgaag gggcaaagcg ggtgacgaca
     1501 ggctcgctca tcgcgagccc tgtaccatag ttagcgggac gagtgtcagc gcgattgaca
     1561 tcgaggcgag cgccacgtca tacgggccgc agggtacggc ttcaagctgt ttgaggaagc
     1621 atggcaaccg atgcaaaggc aagaggtgaa ccggacctga ccgcgccggg tcgtgacttt
     1681 atccgcgaca tcgtcaaggg cgatctcgat gcgggccgag tcgagcaggt tattacgcgc
     1741 ttcccgccgg agcccaatgg ttatctgcac atcgggcacg caaaatcaat ttgcctgaat
     1801 ttcggcattg ccgaggagtt tggcggacat tgccacttgc ggttcgatga cacgaacccc
     1861 gcgaaggagg agcaggaata tatcgacgcg atcgagcacg atgtgcgatg gctcggtttt
     1921 gactggggca agcacctcta ccatgcctcc gattatttcg agcagctcta cgcctgggcc
     1981 gaggatctca tccgcgccgg caaagcctat gtcgacgacc agaccccgga gcagatgcgg
     2041 atcaaccgtg gcaccctgac cgagccgggc aaaaacagcc cgttccggga gcgcgaagtt
     2101 gatgagaacc tggatttgtt ccgccgtatg cgggcgggcg aatttcccaa cggcgctcgc
     2161 gtgctgcgcg ccaagatcga tatgtcgtcg ggtaatatca accttcgcga tccggtcctg
     2221 taccgaattc tgcacgcgcc gcatccacgc accggcaccg cctggaagat ctatcccagc
     2281 tacgactttg cgcacggcca gtcagacgca atcgaaagca ttactcattc gatttgcacg
     2341 ctggagtttg aggaccaccg cccgctctac gactggttca t
//
LOCUS       DASSRL010000650         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1172225, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000650 DASSRL010000000
VERSION     DASSRL010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172225"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VFL49_13320"
     CDS             <1..167
                     /locus_tag="VFL49_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7913008.1"
                     /translation="RKTREKSEAIRRARKLARKKAQREGLIPPDARKKKLLDAKKTGQ
                     RPALRPFPSF"
     gene            complement(630..803)
                     /locus_tag="VFL49_13325"
     CDS             complement(630..803)
                     /locus_tag="VFL49_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913009.1"
                     /translation="MVEYRIFRLETEWHWQVMEVMDHFTTFLASGDASSGHTARIAAL
                     SFCRRYQENYPAV"
     gene            1090..1794
                     /locus_tag="VFL49_13330"
     CDS             1090..1794
                     /locus_tag="VFL49_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7913010.1"
                     /translation="MNPKTNLMLCSLTADARQALKPHLAQVELKQHEIIFDVREAVSK
                     VHFPLDAVISLVLPLSTGEVVETAMVGRDGIVGAAAALNGRVSLNRAVVEIGGQSLAC
                     KIEPLKAVLANQPGMQARLGAHEQVLFAQAQQSAACNITHDIENRLARWLLRAADLVG
                     SDELALTQEYIAEMLGVRRTSVSMVAHMLQQAGMIEYRRGRIKILNLDGLRDTACECY
                     ETVKLNYDALHHQSND"
     gene            complement(1856..2059)
                     /locus_tag="VFL49_13335"
     CDS             complement(1856..2059)
                     /locus_tag="VFL49_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913011.1"
                     /translation="MARLASGGALAQRETQRMVIEKGLTFAEAQLAAAAKMMTGVGIS
                     GATKSASDIYRRKVRANRRRLVR"
     gene            complement(2309..>2580)
                     /gene="purH"
                     /locus_tag="VFL49_13340"
     CDS             complement(2309..>2580)
                     /gene="purH"
                     /locus_tag="VFL49_13340"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438890.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7913012.1"
                     /translation="MSRVDAARIAARKAEDAAKELKLPAPLTKGSVVASDAFFPFPDG
                     LLVAIEAGATAVIQPGGSVNDAKVIEAADAHNIAMAFTGVRHFRH"
BASE COUNT          587 a          748 c          712 g          533 t
ORIGIN      
        1 agcgcaaaac ccgagaaaaa tctgaggcga tccgccgcgc gcgcaaactc gcgcgcaaga
       61 aggcacagcg cgaggggctc attcctcccg acgcacgcaa gaagaagctg ctggatgcaa
      121 agaagaccgg acaacgtccg gcgttgcggc catttccctc attctaaatg gtagttctcc
      181 gagaggccga tgacagatca tcccgatttt tccgttgtcg taaaaagcag gggtcgccct
      241 ccaagcccct ggaaatggga aatttatcgc tccggcagga aaagcccggt taaacaatca
      301 gcggtctttt tcagcacagt aactgaggca aatcgagccg gtagaaaagc actcgacctg
      361 tttctgagcg aattccagga tgaaaagccc atgggatcgg tgccccacgg ctctagtgac
      421 tgatcaaagg ttcttcatgg atgatcttgc cgccagcgtc cgttatcaca atgcgttcat
      481 ccttcggaac cctgctgctt tgtgatagct cgcgcgcggc aagcttcgcc tcttcgcgag
      541 ccgcttcatc gtcgggaaag tcctgtccct caatgtcgcg agcgccggaa ccagatagat
      601 ggaagaaata tcgaggcatg agctggcact tacaccgccg gataattctc ctggtagcgg
      661 cggcagaatg agagagcagc gatccgcgct gtgtgtccgc tggaagcgtc gcccgaagca
      721 agaaatgtcg tgaagtgatc catcacttcc atcacttgcc aatgccactc cgtctccaga
      781 cgaaaaatcc ggtattccac catcggttct gccttccaag ccgcccgcga cgggaatttg
      841 tcgccgataa ggtcaaggca cgcattccgt tgcgatatgg ttactaatgt cgcctggaca
      901 tcatagttgc agaacgcttc gaatttttag gacaaccaac ctcccctaaa aataagctcc
      961 gaaaaagttg gaactatcag cgtgtcgggt tattggctta catcatcggc aagcggccct
     1021 gagcctgcag gcgccgatgg ctgagagacc gttgcgctcc cgcctgtttc ggcaagggga
     1081 gcgctctcaa tgaatcccaa aaccaatctc atgctttgtt cactgacggc tgacgcacgt
     1141 caggcactga aaccgcacct tgcgcaagtc gagcttaaac agcacgagat catctttgac
     1201 gtgcgtgaag cagtttccaa agtacacttt ccgctcgatg ccgtaatttc actcgttttg
     1261 ccgctctcaa ctggagaggt cgtcgaaacc gctatggtgg ggcgtgatgg aatagtcggg
     1321 gcagctgccg cgctcaatgg cagggtgtcg cttaatcgcg cggtggtgga aatcggcgga
     1381 cagtctcttg cctgcaagat cgagccgtta aaggcggtgc ttgcaaacca acctggcatg
     1441 caggcccggc tcggagcaca cgagcaggtc ttattcgcac aggcgcagca atcagccgcc
     1501 tgcaacatta cgcatgatat cgaaaacagg ctcgcccggt ggcttttgcg cgccgccgat
     1561 ttggtcggca gcgatgaact tgcgctaacc caagagtaca tcgcggaaat gctgggagtt
     1621 cggcgtacaa gcgtttcgat ggtcgcgcac atgctgcaac aagccggaat gatcgagtat
     1681 agacgcgggc gcatcaagat tctaaatctc gatggtctgc gggacacggc gtgcgaatgc
     1741 tacgagacgg tcaaacttaa ctacgacgcc ttacaccatc aatcgaatga ctaacaatcg
     1801 gcaaccacat gcgttcttcc gcttgtgacc cgaggcggaa gaacgatgag aacaactagc
     1861 gcaccaggcg acgccggttg gctctcactt ttcgccgata gatgtccgac gctgatttcg
     1921 ttgcgccaga aattccaaca ccggtcatca tcttagccgc agcagccagt tgggcttctg
     1981 cgaacgtaag gcccttttct atgaccattc tctgcgtctc gcgctgcgcc aaagcaccac
     2041 cggaggcgag tcgcgccatt cgcattgcga caacgacgtt ggcatcccat gcggcttgcg
     2101 cgagttgaaa ataagcaggc caaggattga acacgatcta tctccaagtc gggcgttcca
     2161 ttatcgaaaa agtggcgccc aaagtacacg gcaatgtccg ccatcgaccc gtcgcgtcaa
     2221 ttcgttgccg cgcagccatt cggtcgcttt tcgagtgaag caggcatgcg ctgcgctgtt
     2281 cgcagaaccg gctttatgag cacacgcctt agtgcctgaa gtgcctgacg cctgtgaacg
     2341 ccatcgcgat gttgtgggcg tcggctgcct cgatcacttt cgcgtcgttc accgagccgc
     2401 cgggctggat caccgccgtt gcgccggctt cgattgcgac cagcaggcca tcgggaaacg
     2461 gaaagaacgc atcggatgcg acaaccgagc ctttggtgag cggcgccgga agcttgagct
     2521 ccttcgccgc atcctccgcc ttgcgcgcgg cgatgcgggc ggcgtcgacg cggctcatct
//
LOCUS       DASSRL010000651         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1416387, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000651 DASSRL010000000
VERSION     DASSRL010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416387"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..405
                     /locus_tag="VFL49_13345"
     CDS             43..405
                     /locus_tag="VFL49_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913013.1"
                     /translation="MIKRIFFAFFAFIGLIIPAAFGFAVLAAPPANPAPIQLPGCDRN
                     LAQATSSVAAVQARVKGTATKEGPDTCTATRLYFLEVVKARAVTALCRSGPERERELG
                     RLDADVEHINEAIAAHCN"
     gene            542..1390
                     /locus_tag="VFL49_13350"
     CDS             542..1390
                     /locus_tag="VFL49_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913014.1"
                     /translation="MRASARALRTIGALWVMAAPAPAVMPASAQEPTEAAPLSAEDSA
                     LLANALVFDPGALATSPKRPLRIPGYTSSDYNITRTQKFDGSTTVVVKQPVQTEWSNS
                     VGADLAPSQPTPYPLPLPTERNNGMPAGAAWASVGVPNLASVDARVDPANEQGKVGTT
                     LKHSIPFGSRFAMIVQDTYSVTEMLGQPSPGPSDLPLMTLPAPGGPPTQQVFGNEKAV
                     KFNILPTGTTLGAGFTTASNDPVTHNTLSAEQKLYGPLHVTTAVTDLGQSTSNKSITA
                     GFKLNW"
     gene            1573..2034
                     /locus_tag="VFL49_13355"
     CDS             1573..2034
                     /locus_tag="VFL49_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913015.1"
                     /translation="MTAIRPERLGADRTIPEKTGPEAYAPNRKSSERTESEQEAVTAF
                     EGEIREFVRRDVAHLRRQRNDVEQPSEPVMDNLNVLIRRVSGASIDEIDRVILELQGV
                     REMLRSEGERVGREIAGYASLSHAAMTAMKVIGDSLTQWKSAPNNYSRPAE"
     gene            complement(2085..2771)
                     /locus_tag="VFL49_13360"
     CDS             complement(2085..2771)
                     /locus_tag="VFL49_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913016.1"
                     /translation="MKNSHRFLITAGLVAGVTLSSLGVAAFAQKDSINPGLNPGTGQI
                     NTGTEPHPPSQSAEVSKIPTPEEARAAMMQPISQQPSAGNAANGTTGSSGQQPPMQNA
                     SGAANEPPPSGPIGSFGQTIPAKFSKRNDILDNVPIMAMPLRLTDEQRKQIYDAVMAE
                     KSQPVVGADALKPASELSPDQALNGMRPLPESVRGIKNVSRLYYIKAKDKVLLIEPNV
                     RTVVDQITAS"
     gene            complement(2919..3188)
                     /locus_tag="VFL49_13365"
     CDS             complement(2919..3188)
                     /locus_tag="VFL49_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913017.1"
                     /translation="MSMINYRAPSELFPSRSRKSRRPIGYKRFPTAAEAIRFAIEELP
                     PELLLGAYLEVEEKRFDGNGIRALYDSADYPLPRKAVKTKVAAAS"
BASE COUNT          669 a         1063 c         1069 g          602 t
ORIGIN      
        1 ggccggccgc gaccaacggg tggcagcgca ggagggtcgc acatgatcaa gcgcattttc
       61 ttcgcatttt ttgcatttat cggcctgatc attccggccg ccttcggctt cgcggtgctg
      121 gcggcgccgc ccgcaaaccc ggcgccaatc cagctcccag gctgcgaccg caatctcgcc
      181 caggccacga gcagtgttgc ggctgtgcag gcgagagtta agggcaccgc caccaaagag
      241 ggcccggaca cgtgtaccgc gacgcggctc tatttcctcg aggtcgtcaa ggcgcgcgcc
      301 gtcaccgcgc tgtgcaggag cgggccggaa cgggagcgcg aactcggccg cctggatgcc
      361 gacgtggaac acatcaacga ggcgatcgcc gcgcactgta actgactgga aataatccgg
      421 attgcttcac agcttcgcca aagttccatg gcataaggca accaatgctt aaccggtttg
      481 cgcagaacat cgcgcaataa aaaaacgtca ccggacggca ctctcggggg agatttcggg
      541 aatgcgagca agcgctcgtg cgttgcggac gattggcgcg ttgtgggtaa tggcggcgcc
      601 ggcgccggcc gtgatgccgg catcggcgca ggagccgacg gaagccgccc cgctctcagc
      661 ggaagacagc gccctgctgg caaatgccct ggtcttcgat ccgggcgcgc ttgcgacatc
      721 gccgaaaagg ccgctgcgca ttccgggcta cacaagcagc gactacaaca tcacgcgcac
      781 acagaaattc gacggctcga ccacggtcgt cgtgaagcaa cccgtgcaga ccgaatggtc
      841 gaattcggtg ggcgccgatc tcgcaccgtc gcagccgacg ccgtatccgc tgcctttgcc
      901 gacggaacgc aacaacggca tgcccgccgg cgcagcctgg gcgtcggtcg gcgtgccgaa
      961 tcttgccagc gtggatgcgc gcgtcgatcc ggccaacgag cagggcaaag tcggcacgac
     1021 gctgaagcat tcaattccct tcggcagccg tttcgccatg atagtgcagg acacttactc
     1081 agtgacggaa atgctcggcc agccgtcgcc cggcccgagc gatctgccgc tcatgactct
     1141 gcccgcaccc ggcggcccgc cgacccagca ggttttcggc aatgagaagg cggtcaaatt
     1201 caacattttg ccgaccggga ccacgttggg tgccggcttt accaccgcca gcaacgatcc
     1261 ggtcacgcac aacaccctca gcgccgagca gaagctctac ggcccgctgc atgtgacgac
     1321 tgcggtgacc gatctcgggc agagcaccag caacaaaagc atcaccgccg gcttcaagct
     1381 gaactggtag ccggttgcca aataccacgg cgcggtttgg aaaagccgtg cataactggg
     1441 cgcgcggcca cgtttggccg gaatgtatca ccactctgcc gatcccgccc cacggccgcc
     1501 gcagtcgcgt gcggcaatcg gctgctattg ggagcattcg cgcaaattct tgcgcttctc
     1561 gaggagtgga ccatgacggc catcagacct gagcgacttg gtgcggacag gacgattccg
     1621 gaaaagaccg gtccggaggc ttatgcgccg aaccggaaat cttcggaacg gaccgagtcc
     1681 gagcaggagg cggtgaccgc tttcgagggt gagatccgcg agttcgtccg gcgtgacgtg
     1741 gcgcatctgc gccgccagcg caatgacgtc gagcaaccgt ccgaaccggt tatggacaac
     1801 ctcaatgtgc tgatccgccg cgtttccggg gcgtcgatag acgaaattga tcgcgtcatt
     1861 cttgaactgc agggcgtgcg cgaaatgctt cgcagcgaag gcgagcgcgt cggccgggaa
     1921 atcgccggct atgccagcct gagccacgcg gcgatgaccg ccatgaaggt catcggtgac
     1981 agcctgacgc aatggaagag tgcgcccaac aattacagcc ggccagctga atagcgcgcg
     2041 gaatggaatt gccaccgccg gacgttccgg cgatggctcg ccaactagga cgccgtgatc
     2101 tgatcgacaa ctgtgcgtac gttgggctcg atcagcagga ccttgtcttt cgccttgatg
     2161 taataaaggc gcgagacatt tttgattccg cgcacgctct cgggcaatgg acgcatgccg
     2221 ttgagcgcct gatccggcga cagctcgctt gcaggcttga gcgcgtcggc gccgacgacc
     2281 ggctgcgatt tctccgccat caccgcatcg taaatctgct tgcgctgttc gtcggtcaac
     2341 cgcaatggca tcgccatgat cggcacgttg tcgaggatgt cgttgcgctt ggaaaatttt
     2401 gccggaatgg tctgcccgaa tgaaccaatc ggtccggacg gcggtggctc gttcgctgcg
     2461 ccggacgcgt tctgcatcgg cggctgctga ccgctgctgc ctgtcgttcc gttggcggcg
     2521 ttgccggcgg acggctgttg cgagatcggc tgcatcatcg cggcgcgtgc ctcttccggc
     2581 gtcgggattt tactgacttc ggccgactgc gacggcgggt gcggttcggt gccggtattg
     2641 atctggcccg tgcctggatt cagtccgggg ttgatcgagt ctttctgggc gaaagccgca
     2701 acgcccaagg acgacagtgt gacaccggcg acaagaccgg ccgtgattaa aaaacggtgc
     2761 gaatttttca tggggaagcc tccatcggct gctcctggac aacggccgag aggccgtgcc
     2821 ggttgcatgc cgtgtcggcc gcacccggac gggcaggtgc aaaaatatcc aacgccgccc
     2881 gcgcaggcgg acggcggtca gaagcgacag aaagtcagtt agctggcggc cgcgaccttg
     2941 gttttcacag ccttgcgcgg cagcggataa tcggcgctgt cgtacagcgc gcggatgcca
     3001 ttgccgtcga aacgcttctc ctcgacctcg agataggcgc cgagaagcag ctcaggcggc
     3061 aattcctcga tcgcgaagcg gattgcctcc gcggcggtcg gaaagcgctt atacccgatc
     3121 ggacggcgcg acttccggct acggctcgga aacaattcgg acggggcacg gtagttaatc
     3181 atcgacataa tcggtagctc ctcggcccct aatatggccc ttggagcgcc gaaaatccag
     3241 cacttagcga cccgtgcata tcaggccggc aggctcaagg taaatgcgcg gtaccgaaaa
     3301 gcgaaacccg gcgcgcctga cgcgccgggt ccaattctca gcagttggga cgcatttaat
     3361 ccacgaattc gtccacaacc tcgctcgttg ccggatcaac caa
//
LOCUS       DASSRL010000652         5444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1452107, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000652 DASSRL010000000
VERSION     DASSRL010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452107"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFL49_13370"
     CDS             complement(<1..261)
                     /locus_tag="VFL49_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7913018.1"
                     /translation="MKRLATLTALVLAVTAQGVSAQTLKAVKDRGMLNCGANGTLAGF
                     GLPDAQGKWAGLDVDFCRAIAAAVLNDANKVKYVPLSAKDRFT"
     gene            519..1709
                     /gene="metC"
                     /locus_tag="VFL49_13375"
     CDS             519..1709
                     /gene="metC"
                     /locus_tag="VFL49_13375"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET7913019.1"
                     /translation="MAKKTKSPIKPATRLVTAGRDPLANHGFVNPPVYHASTVLYPTA
                     DDQVSHRARYSYGRRGTPTSEALEDALRELDGEGCAGVALLPSGLAAISAALLSVARA
                     GDHVLVTDSVYRPTRNFCNSVFARFGVETTYYDPLIGSDIVKLFKPNTRAVFVEAPGS
                     QSFEMQDIPAIAGIAHDKRATVLMDNTWATPLYFRAFEKGVDLEIQAGTKYIGGHSDI
                     MFGCVSANAATLPALKDTVYAMGICVGPDDMYLALRGLRTLGVRLARHFDSGLRIARW
                     LAQRPEVLRVMHPALERDPGHAIWKRDFTGACGLFSIVLKPASEKAVHAFLNELTLFG
                     LGYSWGGFESLAILFDCSEYRTATKWMPGGPAIRFHIGLEDPDDLTADLERGFAALAK
                     TGAD"
     gene            complement(1785..2114)
                     /locus_tag="VFL49_13380"
     CDS             complement(1785..2114)
                     /locus_tag="VFL49_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HET7913020.1"
                     /translation="MLIDIAQAVGGYLHDVFIGNADWGILIGYIAQMMFAMRFVVQWI
                     ASERAGRSVVPMAFWIFSIGGGLMLLGYALYRKDPVFIIGQAFGVFVYLRNLQFVLRS
                     GGTAAAA"
     gene            complement(2115..2846)
                     /locus_tag="VFL49_13385"
     CDS             complement(2115..2846)
                     /locus_tag="VFL49_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7913021.1"
                     /translation="MTTTDPAVSIVVPVRNEAGNIAPLVEEIAAAVDGHWPFEVVYVN
                     DGSSDTTDDELGSLMRRYDWLRRVRHRQSCGQSAAVRSGVAAARAPLVVTLDGDGQND
                     PVFIPALIRTLEAGAPRTGLVAGQRVARKASGFKKFQSRIANGVRGAVLRDGTRDTGC
                     GLKAFQRDVFLRLPYFDGLHRFLPALVRREGCAIAYVDVVDRPRAHGVSNYGMWDRLW
                     IGILDLAGVWWLIRRKKKIPEISED"
     gene            complement(2858..3712)
                     /locus_tag="VFL49_13390"
     CDS             complement(2858..3712)
                     /locus_tag="VFL49_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7913022.1"
                     /translation="MSLANGFNRMEKETGTVLRRCATNVVAALNVLARPPRIRRAHSK
                     PYSLQMLAIAAGVAIAVFLLLAWTLDAAASRGASRLPHWIVVLFDDITDFGKSGWFLW
                     PLGLLFIALAALPPVLTPFSQRVLAAVMVRVGFLFTAIAVPGLFVSIVKRIIGRARPN
                     VGGSLDPFLFSPLKWVPAYAGMPSGHATTAFAVLVAFGTLWPRARTVLLIYALLIAIS
                     RVVVTAHYLTDVLAGAVVGIAGAMMVRRYFALRRLGFSIGPDGDIHQLPGPSLRRMKA
                     VARNLLAP"
     gene            complement(3709..5325)
                     /locus_tag="VFL49_13395"
     CDS             complement(3709..5325)
                     /locus_tag="VFL49_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7913023.1"
                     /translation="MLFSLLAFLPGFFQIPPVDRDEARFAQATKQMLETGEYVDIRFQ
                     NEVRYKKPVGIYWLQAAAVKGGEALGVPKARTTIWLYRLPSLFGAVGAVLLTYWAALA
                     FVSRRAALIAALMMASSVLLGVEARLAKTDAMLLFASVAAMGAMSRIYLGHRRGPDIG
                     LGWKYPAILWTALAGGILLKGPLILMFVVLAAVTLSIAERSVRWVWSLRPLAGCAWMI
                     LLVLPWFIAIIAKSGGDFFVQAVGHDMLGKVTSGQEAHGAPPGYYFVLFWVTFWPGSV
                     LAGLAAPAIWRSRREAGTQFLLAWLVPSWLVFEAVMTKLPHYVLPLYPAIAVLIAGIL
                     DSGSLSKSRWLVRGAIGWFLFPGAIAIAVLVAFVMFGGDLGLIAWPFSAAAVIFGLFA
                     WWLYDADGAERALLRGMVASVLVAITVYAVALRMLPSLFPSAMIAEDIAMSDCRFAHV
                     ASTFAYQEPSLVFLSGTDTRFTDGAGAADFLRGGSCRFAVVDPRSERAFAQRAESIGL
                     HYSLRQRFEGYNISIGKPVSLTVFRSQESQ"
BASE COUNT         1124 a         1781 c         1663 g          876 t
ORIGIN      
        1 ggtgaagcgg tcttttgccg agagcggcac atatttgacc ttgttggcat cattgagtac
       61 cgcggcggca atcgcgcggc agaaatcgac atcgaggccc gcccattttc cctgcgcgtc
      121 gggcaaaccg aaccctgcga gcgtgccgtt ggcgccgcag ttgagcatgc cgcgatcttt
      181 caccgccttg agcgtctgag cggatacgcc ttgcgcggtg acggcgagga cgagcgctgt
      241 gagtgtggca agtcgcttca tgtggcgttc cttcaaaacc tgttcgtgca aaggcctttt
      301 tacaattctc aaactcatcc gccgccggac gtccgtccgg cggtaacagg cgcttccttt
      361 ggccttgtct gtctcggcag ccccttcttg cgcggcatca cagaacgatc ggcctgccgg
      421 gtcaaggggt tgacgctcgc gcgttgcgcg gacgtatatg tgtttgcgac gggaacatgc
      481 gcggctactg tccggccaac tcattgaata acaatcgtat ggcgaaaaaa acgaaatccc
      541 ccatcaagcc cgctaccagg ctggtcaccg cgggccgcga tcccctggcc aatcacggct
      601 ttgtcaatcc cccggtctat cacgcgtcga cggtcctcta tcccacggca gacgatcagg
      661 tctcccaccg ggcacgctat agctacggcc ggcgcggcac acccacctcg gaagcgctcg
      721 aggatgcgct gcgcgagctc gacggtgaag gctgcgccgg cgtcgccctg ctgccgtcag
      781 gactggcggc aatatcggcg gctctgctct cggtcgcgcg cgccggcgat catgtccttg
      841 ttaccgacag cgtctatcgt ccaacgcgga atttctgcaa cagcgtgttc gcgcggtttg
      901 gagtggagac gacgtattac gatccgctga tcggcagcga tattgtgaag ctgttcaagc
      961 cgaacacgcg tgccgtgttc gtcgaggcac ccggttcgca aagcttcgag atgcaggaca
     1021 ttccggccat cgccggtatt gcgcatgaca aacgcgcgac cgtgttgatg gacaatacct
     1081 gggcgacgcc gctttatttc cgcgccttcg aaaaaggcgt cgatctcgaa atccaggcag
     1141 gcaccaaata tattggcggt cattccgaca ttatgttcgg ttgcgtgtcg gcgaatgcgg
     1201 caacgttgcc ggcgctgaag gacactgttt atgcgatggg gatttgcgtc ggtccggacg
     1261 acatgtacct cgcgctacgc ggcctgcgca cgctgggtgt gcggctcgcg cgccattttg
     1321 actccggcct gcgtatcgcg cgctggctcg cgcaacggcc ggaagtcctg cgcgtgatgc
     1381 atcctgcgct ggaacgcgac ccaggccacg ccatctggaa acgggatttc accggcgcct
     1441 gcggactctt cagcattgtc ctcaagccgg cgagcgagaa ggccgtgcat gccttcctca
     1501 acgaactcac tttgttcgga ctcggctatt cgtggggcgg cttcgaaagc ctcgcgattc
     1561 tgttcgactg cagcgagtac cggaccgcaa ccaaatggat gccgggtggc ccggcgatcc
     1621 gtttccatat cggcctcgaa gacccggacg atctgactgc cgacctcgag cgaggctttg
     1681 cggcgctcgc gaaaactggt gctgactaga tcagaatccg ttgagattga aactatacgc
     1741 cgtcatggcc ggactagttc cggccatgac gaaacgatcc tgccctacgc cgccgccgca
     1801 gtgccgccac tccgcaacac gaattgcaga ttgcgcagat agacaaagac gccgaacgcc
     1861 tgcccgataa tgaagaccgg gtccttgcgg taaagcgcgt agccgagcag catcaatccg
     1921 ccgccgatcg agaaaatcca gaatgccatc ggcacaacgc tgcggcccgc tcgctcggag
     1981 gcaatccatt ggaccacaaa gcgcatggca aacatcatct gcgcgatata gccgatgagg
     2041 atcccccagt cggcattgcc gatgaacacg tcgtgcagat agccgccgac ggcttgcgcg
     2101 atatcgatca gcatctagtc ctccgaaatt tccggaatct ttttcttgcg gcggatgagc
     2161 caccacacgc cggccagatc gagaatgccg atccagagcc ggtcccacat tccatagttc
     2221 gacacgccat gcgcgcgcgg ccgatcgacg acgtcgacat aagcgatggc acacccttcc
     2281 cgccgcacca gcgccggcag aaagcgatgc agaccgtcga aatacggcag gcgtaaaaag
     2341 acatcacgct ggaacgcctt caggccgcag ccggtgtcgc gcgtgccgtc gcgcagcacg
     2401 gcgccgcgca cgccgttggc aatccgcgac tggaatttct tgaagccgct ggccttgcgc
     2461 gccacacgct gcccggccac gagcccggtg cgtggcgcgc ccgcttcgag cgtgcggatg
     2521 agcgccggaa taaacaccgg atcgttctgc ccgtcgccgt cgagcgtcac caccagcggc
     2581 gcacgcgccg ccgcaacgcc gctgcgcacc gcagccgact gcccgcagga ttgccggtgg
     2641 cggacgcggc gcagccagtc gtagcgccgc ataaggcttc caagctcgtc gtccgtcgta
     2701 tcgcttgagc cgtcattgac gtagacgacc tcgaaaggcc aatggccatc gaccgccgcc
     2761 gcgatttcct cgaccaaagg cgcgatgttg cccgcttcat tgcggaccgg caccacaatg
     2821 gacaccgccg gatcggttgt cgtcatggcg cgatgtctta tggggcgagc aggttgcggg
     2881 caaccgcttt catacgccta agcgatggcc ccggaagctg atggatgtca ccgtccggcc
     2941 cgatcgaaaa gccgagtcgg cgcagcgcga aatagcgccg caccatcatt gcaccggcaa
     3001 tcccgaccac tgcccccgcg agcacgtcgg tcaggtagtg cgcggtcacc accacacggc
     3061 tgatggcaat taaaagggca tagatcagca ggaccgtgcg tgcgcgcggc catagcgtgc
     3121 cgaaagcgac cagcaccgcg aaagccgtcg tggcgtgccc ggatggcatg ccggcgtaag
     3181 ccggcaccca tttgagcgga ctgaagagga acggatcgag gctgccgccg acgtttggcc
     3241 gggcgcggcc gatgatgcgc ttgacgatac tgacaaacag gccaggaacc gcgatcgcag
     3301 tgaacagaaa gccgacccgc accatgaccg ccgcgagcac gcgctgcgag aacggcgtga
     3361 gcaccggcgg caaggcggcg agcgcgataa acagcagacc gagcggccac aggaaccatc
     3421 cggacttgcc gaagtcggtg atgtcatcga acaacacgac gatccaatgc ggcaggcggc
     3481 tcgcgccacg gctcgccgcc gcgtcgagcg tccacgccag aagcaggaag acggcaatgg
     3541 cgacgccggc cgcaatcgcc agcatctgta aggaatacgg cttggagtgc gcccgccgga
     3601 ttcgcggcgg gcgcgccaac acattgagcg ccgcaacgac attagtcgcg catcggcgga
     3661 gcactgtgcc ggtttccttt tccattcggt tgaaaccgtt ggccagactc attggctctc
     3721 ctgcgaccgg aagacggtca acgagaccgg cttgccgatg ctgatattgt atccctcaaa
     3781 gcgctgcctg agcgaatagt gcaacccgat ggattccgcc cgttgcgcga aagcgcgctc
     3841 actgcgcgga tccacgaccg cgaaacggca ggacccgccc cgcaggaagt cggcggcccc
     3901 tgcaccgtcg gtgaaacgcg tgtcggttcc cgagagaaac accaggctcg gctcctgata
     3961 ggcgaatgtc gaggcgacgt gggcgaaacg gcagtcggac atggcgatgt cttcggcgat
     4021 catcgcgctc ggaaacagcg acggcagcat gcgcaatgcc accgcgtaga ccgtgatcgc
     4081 gaccagcacc gaagcaacca tcccacgcaa cagcgcgcgt tccgccccgt cggcgtcgta
     4141 tagccaccag gcgaacagtc cgaatatcac ggccgccgcg gaaaacggcc acgcgatgag
     4201 cccgaggtcg ccgccgaaca tgacgaaggc gacgagcacc gcaatcgcga tagcgccggg
     4261 aaacaggaac cagccgatgg cgccgcgcac cagccagcgc gatttcgaca ggctgccgga
     4321 atcaaggatg ccggcgatca gcaccgcgat cgccgggtaa agcggcagga cgtagtgcgg
     4381 caacttggtc atcaccgcct cgaataccag ccaggacggc acgagccagg ccagaagaaa
     4441 ctgcgtgccg gcttcgcggc gcgatcgcca gatcgccggc gccgccagcc ccgcgagcac
     4501 cgaacccggc cagaacgtga cccagaacag cacaaaataa tatcctggcg gtgcaccgtg
     4561 cgcttcctgc ccgctggtca ccttgccaag catgtcgtgg ccgaccgcct gtacgaaaaa
     4621 gtcgccgccc gacttggcaa tgatcgcgat gaaccatggc aggaccaaca ggatcatcca
     4681 ggcacatccg gcaagcggac gcagcgacca cacccaacgc accgagcgct cggcaatcga
     4741 taatgtgaca gcggcgagca cgacgaacat caggatcagg gggcctttaa gaagaatccc
     4801 gccggccagc gctgtccaga gaatggccgg gtatttccag ccgagcccaa tgtcgggccc
     4861 tcgcctgtgc ccaagataga tgcgtgacat ggcgcccatg gccgcgaccg atgcaaacaa
     4921 cagcatcgcg tctgtcttgg cgagccgtgc ctcgacgccg agcaggaccg agcttgccat
     4981 catcagcgcc gcgatcagcg ccgcgcgccg cgaaacaaaa gcgagcgcgg cccagtaggt
     5041 cagcagcacc gcgccgaccg caccaaagag tgacggcaga cgatagagcc agatggtcgt
     5101 ccgcgctttc ggcaccccga gcgcttcgcc gccttttacc gcagctgcct gcagccagta
     5161 gatgccgacc ggcttcttgt agcggacctc gttctggaag cggatgtcga catattcacc
     5221 ggtttccagc atctgcttgg tcgcctgggc aaagcgggcc tcatcgcggt ccacaggcgg
     5281 gatctggaaa aagcccggca ggaaggccag caaggaaaac agcacgagca gcgcaactgc
     5341 gcgtccatgc gatgcgaccg cacggtcgag cgccgcggca aggccgcggc ccggaacaaa
     5401 ggatgggccg gccgtatctg tcgtggttct cactctcgaa gccc
//
LOCUS       DASSRL010000653         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_104879, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000653 DASSRL010000000
VERSION     DASSRL010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104879"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VFL49_13400"
     CDS             complement(<1..1159)
                     /locus_tag="VFL49_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7913024.1"
                     /translation="MKLLLRFFGFLFAAGTILFVVGVAAAAGLLWHFSKDLPDYSQLQ
                     DYEPPVMTRVHAADGSLVAEYARERRLYIPIQAVPKMVINAFLAAEDKNFYEHGGLDF
                     TGIARAGINYLQNYGSSRRPQGASTITQQVAKNFLLTNELSISRKLKEALLALKIERT
                     YSKDKILELYLNEIYLGLGAYGIAAASLTYFDKAVNELTVPEAAYLASLPKAPSNYHP
                     FRQRERAIERRNWVLDRMAEAGFVKTADAEKAKRTPLGVTTKSSSAHTFAGEYFAEEV
                     RRELYERYGEKKLYEGGLSVRTSLDPKLQVLARKTMIDGLVRFDQTQGWRGPVTKIDI
                     AGDWGVKLAEVKALSDVAPWRLAGVLEVSDQSARIGLQPGREPGGYVSKERT"
     gene            complement(1279..2538)
                     /locus_tag="VFL49_13405"
     CDS             complement(1279..2538)
                     /locus_tag="VFL49_13405"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7913025.1"
                     /translation="MTARALAIVSALVCALLCDSAGFATESAVTEHVLAAAVEQEVVA
                     TDLRIGGDDKQTRFVVDLNRKIDLAAFTLADPYRVVVDLPQVNFKLPAKTGAQSRGLV
                     KAFRYGLIMQGGSRIVLDTKGPVRIEKAFVLDAAEGQPARLVLDLAAIDRAAFMRNIS
                     LQTRPPQNTGARPSEPPVTADGDTRPLVVVDPGHGGIDNGTKGSGGELEKDIVLAFSQ
                     ALREKLESGGKYRVAMTRSDDTFIPLAERVRFARTRNAALFISIHADALPRKEGYAEG
                     ATVYTLSETASDAEAARLAEAENKADVIAGVDLTAEPGDVANILVDLAQRETKTFSMQ
                     FARTVVDELKTSARLHKHPLKSAGFKVLTAPDVPSVLIELGYMSTKDDLKQLNSPAWR
                     ARTAQAVVKAVEAYFTPRVAGAGEGRR"
     gene            complement(2734..>3160)
                     /locus_tag="VFL49_13410"
     CDS             complement(2734..>3160)
                     /locus_tag="VFL49_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913026.1"
                     /translation="TRVSSGWVASINILSAIGKSHWAANSRGLAHSRGRKSYVRGSGL
                     IGVEGKGRAAKVDTIARRQDGGAWAEDLKIPLLRQWQHETCDPYRCGRALRREPRFLI
                     AHSQVANGCKPRADPGAFTRVSARFTWPSADLLGGRYWA"
BASE COUNT          579 a          987 c          973 g          621 t
ORIGIN      
        1 cggtgcgttc cttgctgaca tagccgcccg gctcccgccc gggctgcagc ccgatgcggg
       61 ccgactgatc actgacctcc agcacccccg cgagccgcca tggcgcaacg tcggacagag
      121 cctttacctc ggcgagcttg acgccccagt cgccggcgat atcgatcttg gtcaccggcc
      181 cgcgccagcc ttgcgtttga tcaaagcgca ccaggccgtc gatcatggtc ttgcgagcca
      241 gaacctgcag cttcggatca agcgatgtgc ggaccgacag tccgccttca tataatttct
      301 tttcgccgta gcgctcatag agctcgcgcc gcacttcctc ggcaaaatat tcgccggcga
      361 aggtatgcgc gctgctcgat ttcgtcgtga cgccgagcgg cgtgcgcttt gccttctccg
      421 cgtcggcggt tttcacaaag ccggcctccg ccatgcgatc aagcacccag ttacggcgct
      481 cgattgcgcg ctcgcgttgc cggaacgggt gataattgct cggcgccttg ggcagcgacg
      541 caagataggc cgcctcgggc acggtcagtt cgttcaccgc tttgtcgaaa taggtcagcg
      601 atgcggcggc aatgccgtat gcgccaaggc cgagatagat ctcgttcagg taaagctcga
      661 ggatcttgtc cttgctgtac gtgcgctcga ttttgagagc gagcagcgct tccttgagct
      721 tgcgggaaat ggaaagctcg ttggtcagca ggaagttctt ggcgacttgc tgcgtgatcg
      781 tcgaagcgcc ctgcggccgc cggctcgagc cgtagttctg cagatagttg atcccggcgc
      841 gcgcgatgcc ggtgaaatcc agcccgccgt gctcgtagaa gttcttgtcc tcggcggcga
      901 ggaaggcgtt gatcaccatc ttggggacgg cctggatcgg aatatagagg cgccgttcgc
      961 gcgcatattc cgcgaccagc gagccgtcag cggcatgcac ccgcgtcatc accggcggct
     1021 cgtaatcctg cagctgcgaa tagtccggca agtccttgga aaaatgccac aggagaccgg
     1081 cggcggccgc cacgcccacc acaaacaaga tggtgccggc cgcaaacagg aagccgaaga
     1141 agcgcagcaa gagcttcatg atcgcatgat cctgtgtcgc gcccggtatt ttatcacgac
     1201 ccacccgact atacccggca agcggccata agcctgtgtt tgctgggttt tatgttgaaa
     1261 ctgaggccgg tcgcacatct agcggcgtcc ttccccggct cccgcgaccc gcggcgtgaa
     1321 gtaagcctca acggccttaa ccacggcctg cgccgtgcgc gcgcgccagg ccggggagtt
     1381 gagctgcttg aggtcgtcct tcgtcgacat gtaaccgagt tcgatgagca ccgaaggtac
     1441 atccggagct gttaacactt taaagccggc cgacttcagc gggtgcttgt gaaggcgcgc
     1501 cgaagttttg agctcatcga caaccgtgcg ggcaaattgc atagagaagg tttttgtctc
     1561 gcgctgcgcc aggtcgacaa gaatgttggc gacgtcaccc ggttcggccg tcaagtcgac
     1621 gccggcaatg acgtcggcct tgttctccgc ctcggcgagc cttgcggcct cggcatcgga
     1681 agcagtttcc gagagcgtat agacagtggc cccctcggcg taaccttctt tgcgcggcaa
     1741 agcgtccgca tgaattgaaa tgaaaagggc ggcattgcgc gtgcgcgcga agcgaacccg
     1801 ctcggccagc ggtataaagg tgtcgtcaga acgggtcatt gcgacgcggt actttccgcc
     1861 gctttcaagc ttctcgcgaa gcgcctgcga gaacgcgagc acgatgtcct tttcgagttc
     1921 tccccccgac cctttcgtgc cgttgtcgat cccgccgtga ccgggatcca cgacgaccag
     1981 cggacgcgta tcgccgtccg ccgtgacggg cggctcgcta ggtctggccc cggtattttg
     2041 cggagggcgc gtttgcagcg agatgtttcg catgaaagcg gcgcgatcga ttgccgcaag
     2101 gtcgagcacc agccgggctg gctggccttc cgcagcgtcc aatacgaaag ctttctcgat
     2161 acgcaccggt cccttggtat cgagcacgat gcgcgagccg ccttgcatga tcagcccgta
     2221 gcggaacgcc ttgaccaggc cgcggctctg ggcgccggtt tttgccggca gtttgaagtt
     2281 gacctgcggc aggtcgacga cgaccctgta gggatcagcc agcgtaaagg cggcgaggtc
     2341 aatcttccgg ttgaggtcga ccacgaaccg ggtctgtttg tcgtcaccgc cgatacgcag
     2401 gtctgtcgcg acgacctcct gttccacggc ggctgcaagc acatgttcag tgacagcgct
     2461 ttcggtggcg aacccagccg aatcgcatag cagtgcgcaa accagggcgc tgacgatggc
     2521 aagagcgcgg gctgtcacga ttccgaacct ttgatagcgc gctttttaac ccattaaggg
     2581 cgggttcggt taacggatat tgatgagagg cgcgattccc cgcgccggtg ttgccttact
     2641 gcaaagcttt gcttgccagt acggccgccg ggccgtatgt aagtcacgct gacggttaga
     2701 gttccggttg tgccatgttc gggccccggc cgcttaggcc cagtaccgcc ccccgaggag
     2761 gtcggcggac ggccaagtga aacgagccga aacccttgtg aaggcacccg gatcagcgcg
     2821 cggcttgcag ccgttcgcaa cctgactgtg agcaatgaga aacctcggtt cgcgcctcaa
     2881 ggcgcggccg cagcgatacg ggtcgcaagt ttcatgctgc cactgacgca gcaaaggtat
     2941 tttgaggtct tcagcccacg cgccgccatc ctgacggcgc gcgatggtat cgaccttggc
     3001 cgcgcggccc ttcccttcaa cacctatcag gccggatccc cggacatacg acttgcgccc
     3061 tctgctatgc gcgagcccgc gggagttcgc ggcccagtga gattttccaa tggccgacaa
     3121 aatgttgatc gacgcgaccc acccggagga gacccgagtc
//
LOCUS       DASSRL010000654         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_402359, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000654 DASSRL010000000
VERSION     DASSRL010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402359"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFL49_13415"
     CDS             complement(<1..844)
                     /locus_tag="VFL49_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET7913027.1"
                     /translation="MTYLAKPKFHHPDLPKNSLGYTHRDYEGTVSTLCAGCGHDSITG
                     AIIQSCFELSIEPHRVAKISGIGCSSKTPTYFLGNSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHCLRRGVNMVYIVENNGVYGLTKGQFSATADRGSKS
                     KRGVINTDNSIDLVAMALQLGASFVARSFSGDKQQLVPIIKAAIAHKGAAFIDVISPC
                     VMFNNHAGSTKSFDFVREHNEAVNRLDFISSRMPIEVKYEPGTVEIVEQHDGSRLALR
                     KLDAD"
     gene            complement(841..2685)
                     /locus_tag="VFL49_13420"
     CDS             complement(841..2685)
                     /locus_tag="VFL49_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET7913028.1"
                     /translation="MPTATPISRVNDFVIRFANVNGSGSASANEMFARAVMRMGVPVA
                     PRNIFPSNIQGLPTWYEVRVSEAGHLGARGGTDLMVAMNPQTFDKDIAGIEPGGYLFY
                     DSTKPLPASKFREDITVIGVPLTAICNREYTDPRQRQLFKNIIYIGALTALLDMDVKV
                     VEQLIGEQYKGKDKLIEPNLKALHIGRDYAILNLQCPLGVKLRRANAVGDRIFIEGNS
                     AAALGAVYGGATVCAWYPITPSSSLADAFTRHCAKLRIDPETKQAKYAIIQAEDELAS
                     IGIVIGAGWNGARAFTATSGPGISLMQEFIGLAYFAEIPAVLFDVQRAGPSTGMPTRT
                     QQCDVTSCAYASHGDTKHVLLLPEDPTESFEFGAQAFDLADRLQTPIFVMLDLDIGMN
                     HHLSKPLAWNDTRKYDRGKVMTAEELEAGREFGRYLDVDGDGIPYRTYPGTHPTKGSF
                     FTRGTSRDRYARYTEEGAPYVDNMQRLIRKFDTAKDLVPRPLIATAGKPTKYGVIYYG
                     STSPAMDEAIHMIEAHGHALDRMRVRAFPFHSSVNSFVADHDFVFVVEQNRDAQLRSL
                     IVNECGIDPVRLVPILHYDGTPITARFIAKSIGDHLDKLTVKPMKVTS"
     gene            complement(2655..>3805)
                     /locus_tag="VFL49_13425"
     CDS             complement(2655..>3805)
                     /locus_tag="VFL49_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7913029.1"
                     /translation="IHIGIDWLSSVSFGHIDKIGKRVVVLGGGNTAMDCCRSARRLGG
                     DQVDVVVRSGFEEMKASPWEKEDAMHEGIPIHNYLVPKEFTHENGKLTGITFEKVKAE
                     YDAKGRRKLVSAGEPDQHFPCDDVLVAVGQENAFPWIERDIGIEFDQWDMPKVDTKTM
                     ASTHPKIFFGGDAAFGPKNIIWAVAHGHEAAVSIDKMLNGEDIADRPAPAVEMMSQKM
                     GIHEWSYDNEIALDKRYKVPHRDKVIALKDIKAEVELGFDDKLAFAEAGRCLNCDVQT
                     VFSAQLCIECDACVDICPMDCITFTPNGEEADLRKRLNAPSHNLAQDLYVQDGLKTGR
                     VMVKDEDVCLHCGLCAERCPTGAWDMQKFLLDMTHAGNSCRPPRRSAA"
BASE COUNT          702 a         1109 c         1225 g          769 t
ORIGIN      
        1 aatcggcatc gagcttgcgc agcgccaacc tggaaccgtc atgctgctcg acaatttcga
       61 cggtgcccgg ctcatatttc acttcgatcg gcatccggct ggagatgaag tcgagccggt
      121 tcacggcctc attgtgctcg cgcacgaaat cgaagctctt ggtcgagccg gcatggttgt
      181 tgaacatgac gcaagggctg atcacgtcga tgaaggccgc gcccttatgc gcgatcgcgg
      241 ccttgatgat cggcaccagc tgctgcttgt cgcccgagaa cgagcgggcg acaaagctcg
      301 cgcccagctg cagcgccatc gcgacgagat cgatggaatt gtcggtgttg atgacgccgc
      361 gcttcgattt cgagccgcgg tcggcggtgg ccgagaactg gcctttggtg aggccgtaaa
      421 cgccattgtt ttcgacgata tagaccatgt tgacgccacg gcgcaggcag tgcgcgaact
      481 gcccgaggcc gatcgaggcg ctgtcgccgt cgccggatac cccgagatag atcaggtcgc
      541 ggttggcaag gttggcgccg gtcagaaccg agggcatgcg gccatgtacg gaattgaagc
      601 cgtgcgaatt gccgaggaaa taagtcggcg tcttcgatga gcagccgatg cccgaaatct
      661 tggcgacgcg atgcggctcg atcgacagct cgaagcagga ctggatgatc gcgccggtga
      721 tcgagtcatg cccgcaaccc gcgcacaacg tcgacaccgt gccctcatag tcgcggtgcg
      781 tatagcccag cgaattcttc ggcagatccg gatgatggaa tttcggcttg gcaagatagg
      841 tcatgacgtc accttcatcg gtttcaccgt cagcttgtcg agatgatcgc cgatcgactt
      901 ggcgataaaa cgggcggtga taggcgtgcc gtcatagtgc aggatcggca ccagccgcac
      961 ggggtcgatg ccgcattcgt tgacgatcaa cgaacgcagc tgcgcgtcgc ggttctgttc
     1021 gaccacgaac acaaagtcgt ggtcggcgac gaaactgttg acgctcgaat ggaacggaaa
     1081 agcgcgcacc cgcatacggt caagcgcgtg cccatgcgcc tcgatcatat ggatcgcttc
     1141 atccatcgca ggcgatgtcg aaccgtaata gatcaccccg tatttggtcg gctttccggc
     1201 cgtcgcgatc agcggtcgcg gcacaaggtc cttggcggtg tcgaacttgc ggatcagccg
     1261 ctgcatgttg tcgacgtaag gcgcgccttc ttccgtgtag cgcgcatagc ggtcgcgcga
     1321 ggtgccgcgc gtgaagaacg accccttggt cggatgtgtg ccggggtaag tgcggtaggg
     1381 aatgccgtcg ccgtcgacgt cgagatagcg cccgaattcc ctgccggctt cgagttcttc
     1441 tgcggtcatg accttgccgc ggtcgtactt gcgggtgtca ttccaggcga gcggcttcga
     1501 caggtgatgg ttcatgccga tgtcgaggtc gagcatgacg aaaatcggcg tctgcagccg
     1561 gtccgcaagg tcgaaggcct gggcgccgaa ttcaaagctt tcggtggggt cttccggcaa
     1621 cagcagcaca tgcttggtgt cgccgtgcga ggcgtaggcg caagaggtga cgtcgcactg
     1681 ctgcgtgcgc gtgggcatgc cggtggacgg ccctgcgcgc tggacgtcga agagaacagc
     1741 aggaatttca gcgaaatagg cgagtccgat gaactcctgc atgagcgaga tgccggggcc
     1801 cgaggtcgcc gtgaaagccc gcgccccgtt ccagccggcg ccgatcacaa tgccgatcga
     1861 ggccagttcg tcttccgcct ggatgatcgc gtatttggcc tgcttggtct ccgggtcgat
     1921 gcgcagcttt gcgcagtgac gcgtgaacgc atcggcgagc gaggacgacg gtgtgatcgg
     1981 ataccaggcg cagacggtcg cgccgccata gacggccccc agtgcggcgg cggagttgcc
     2041 ctcgatgaaa atccgatctc cgacggcatt ggcacgcctg agcttgacgc cgagcgggca
     2101 ctgcagatta aggatcgcgt agtcgcggcc gatatgcaga gccttgaggt tgggctcaat
     2161 cagcttgtcc ttgcctttgt attgctcgcc aatgagttgc tcgacgacct ttacgtccat
     2221 gtcgaggagg gcggtcagcg cgccgatata gatgatgttc ttgaacagct ggcgctggcg
     2281 cggatcggtg tattcgcggt tgcagattgc ggtcagcggc acgccgatga cggtgatgtc
     2341 ctcgcggaat ttcgaggccg ggagcggctt ggtggaatcg tagaacaggt agccgcccgg
     2401 ctcgatgccg gcgatgtcct tgtcgaaggt ctgcgggttc atcgccacca tcaggtcggt
     2461 gccgccgcgg gcaccgaggt gtcccgcttc cgagacgcgg acttcgtacc aggtcggcag
     2521 gccctgaatg ttcgacggga agatattacg cggggcaacc ggaacgccca tgcgcatcac
     2581 cgcgcgggca aacatctcgt tcgcactcgc cgagcccgag ccgttgacgt tggcgaagcg
     2641 tataacgaag tcgtttacgc ggctgatcgg cgtggcggtc ggcatgaatt ccctgcgtga
     2701 gtcatatcga gcaagaattt ctgcatgtcc caggcgccgg tcgggcagcg ttcggcgcac
     2761 aggccgcaat gcaggcagac gtcctcgtcc ttgaccatga cacggccggt tttgaggccg
     2821 tcctggacat agaggtcctg cgccagattg tgcgatggcg cgttcagccg cttgcgcaga
     2881 tcggcttcct cgccattcgg agtgaaggtg atgcagtcca tcggacagat gtcgacgcag
     2941 gcgtcgcatt cgatgcagag ctgcgccgaa aacacggtct gcacgtcgca attcaggcag
     3001 cggccggctt cggcaaaggc aagcttgtca tcgaagccga gttcaacctc ggccttgatg
     3061 tccttgagcg cgatcacctt gtcgcggtgc ggcaccttgt agcgcttatc gagtgcgatt
     3121 tcgttgtcgt agctccactc gtgaatgccc attttctggc tcatcatctc aaccgccgga
     3181 gcagggcgat cggcgatgtc ctcgccattg agcatcttgt cgatcgagac cgcggcctca
     3241 tggccgtgcg cgacggccca gatgatgttc ttcgggccga aagccgcatc gccaccgaag
     3301 aatattttcg gatgcgtcga tgccatggtc ttggtgtcga ccttcggcat gtcccattga
     3361 tcgaactcga tgccgatgtc gcgctcgatc caggggaatg cgttctcctg accgactgcg
     3421 accagcacat cgtcgcatgg aaaatgctga tccggctcgc cggcggaaac gagcttgcgc
     3481 cggcccttgg cgtcgtactc ggccttcacc ttttcgaagg tgatgccggt cagcttgccg
     3541 ttctcgtgcg tgaattcctt cggcacgagg taattgtgga tcgggatgcc ctcgtgcatg
     3601 gcgtcttcct tttcccaagg cgacgccttc atttcttcaa agccggagcg aacgacgaca
     3661 tcgacctgat cgccgccgag ccggcgcgcg gagcggcagc agtccatcgc ggtattgccg
     3721 ccgccaagca cgaccacgcg cttgccgatc ttgtcgatgt ggccgaacga gacggacgaa
     3781 agccagtcga tcccgatatg gatat
//
LOCUS       DASSRL010000655         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_1609532, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000655 DASSRL010000000
VERSION     DASSRL010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609532"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFL49_13430"
     CDS             <1..689
                     /locus_tag="VFL49_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HET7913030.1"
                     /translation="LVGMDLVPMVTSAQRFNWDETPWAWNKGYARQTNPEFHVVAVDY
                     GIKRNILRLLAGTGCKVTVVPAKTSAEDIMALKPDGVFLSNGPGDPAATGEYAVPVIR
                     KLIDSGTPTFGICLGHQMLGIAVGGKTMKMHQGHHGANHPVKDLTTGKVEITSMNHGF
                     AVDKDTLPKNVEQTHVSLFDGSNCGLALKDKPVFSVQYHPEASPGPRDSHYLFTRFVE
                     MMRQAKRKIA"
     gene            841..1359
                     /locus_tag="VFL49_13435"
     CDS             841..1359
                     /locus_tag="VFL49_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913031.1"
                     /translation="MSPTPLTFLFKDDGKVPNNPALPALVYKGAVEFGANDPARMIED
                     LFAQNGWGHGQWRNGIYPFVHYHSMIHEALGIARGSARVQLGGHGGETFDFAAGDVAV
                     LPAGTGHQWLSGSDDLLVIGAYPPEGTYNLCRGDNPADRDKALMTIPKVPVPQNDPVL
                     GKDGGLAKLWRG"
     gene            1542..2237
                     /locus_tag="VFL49_13440"
     CDS             1542..2237
                     /locus_tag="VFL49_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7913032.1"
                     /translation="MSDWVKFYDFKHSVIYVNARHRDVHYRTIAEDIRGYVPSRDAHV
                     LDYGCGEATSADLIADACAQLVLVEAAPNVRASLTMRYAGHPKIAVIAAEQAAALQKA
                     SFDLVVMHSVAQYLTQAELDRLLAVFRTLLKPGGLFILGDIVPPHFAAPAAALSLLRF
                     GAANGFFRAAAFGLVRIFASDYLRLRKTAGLSHYSETAMLERLSKAGFKAERAARNIG
                     HNQRRMTFLCRVA"
     gene            2327..2402
                     /locus_tag="VFL49_13445"
     tRNA            2327..2402
                     /locus_tag="VFL49_13445"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2359..2361,aa:Ala,seq:tgc)
     gene            2513..3496
                     /locus_tag="VFL49_13450"
     CDS             2513..3496
                     /locus_tag="VFL49_13450"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7913033.1"
                     /translation="MRRREFLAFIGGAFVATPLAAHAQGERMRRIGVLLPSGADDPEY
                     EARMKAFTERLAQLGWVEGRNIQIDKRFGVAIAEQTRKFAVELVALAPDVILAPGSSA
                     TGPLLQATGTIPIVFATIPDPVAAGYVESLSRPGGNATGFASFEYSIGGKWLELLKEI
                     APGVTRVAVIRDNTTPAGVGQVSAVQTAASSLHVETSVINFRSPGELEKAVAAFARPS
                     SALLVSQGGGTVVHRKLIIALAAKHRLPAVYPGRHFVAIGGLTSYGPDRADQYRQAAG
                     YVDRILKGEKPADLPVQTPSKYELVINLKTAKALGLGVPPSLIARADEVIE"
     gene            3673..3969
                     /locus_tag="VFL49_13455"
     CDS             3673..3969
                     /locus_tag="VFL49_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7913034.1"
                     /translation="MTAFNAVRFKVRPGREQEFLDAHKKVRADWPGLRHANMIKTGDR
                     TFCIIAEWTDMDALTNARPHMVATLDSFRATLEDLGGGLGVTDPVSGPVVLALK"
     gene            3998..>4242
                     /locus_tag="VFL49_13460"
     CDS             3998..>4242
                     /locus_tag="VFL49_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7913035.1"
                     /translation="MLKPRGLFAIVNWHQLPREKTTVPGEPRAPRSELRLSPDLTVKA
                     VEATGLETKAIVDISPYLRRDVRESGPVLVLRPVRFSP"
BASE COUNT          823 a         1316 c         1303 g          800 t
ORIGIN      
        1 gactggtcgg tatggatctc gtgccaatgg taacaagcgc gcagcgtttc aattgggatg
       61 agacgccgtg ggcctggaac aagggctacg cgcggcagac caatccggaa ttccacgtgg
      121 tcgcggtcga ctacggcatc aagcgcaata ttttgcgtct gctcgcgggc accggctgca
      181 aggtgaccgt tgtgccggcg aagacgtcag cggaagacat catggcgctc aagccggacg
      241 gcgtcttcct ctcgaacggc cccggcgatc cggcggcaac cggcgaatat gccgtgccgg
      301 tgatcaggaa gctgatcgac agcgggacgc cgaccttcgg catctgcctc ggccaccaga
      361 tgctgggaat cgctgtcggc ggcaagacca tgaagatgca ccagggtcac cacggcgcca
      421 atcatccggt gaaggatctc accaccggca aggtcgagat cacctcgatg aaccacggct
      481 tcgcggtcga caaggacacg ctgcccaaga atgtcgagca gacgcacgtg tcgctgttcg
      541 acggctccaa ttgcgggctc gcgctcaagg acaagccggt cttctcggtg cagtaccatc
      601 cggaagcctc gcccggcccg cgcgacagtc attatctctt cacgcgcttc gtcgagatga
      661 tgcggcaggc gaaacgcaag attgcctagg ccgcgccaga gtctcccgct cattcccgcg
      721 aaatcgggaa tccagtgccc gatcgcaagc cactgcgcta ttcgccctgg atccccgctt
      781 cgcggggatg agcggaaata agggcaagca actctgcgtg ccgaagattg ttgtaacaaa
      841 atgtcaccca cacccctcac tttcctgttc aaggacgacg gcaaagtgcc gaacaacccg
      901 gcattgccgg cgctcgtcta caagggcgcg gtcgaattcg gcgccaacga tcccgcgcgg
      961 atgatcgaag acctgttcgc gcagaacggc tggggtcacg gccaatggcg caacggcatc
     1021 tatccattcg tgcactatca ttcgatgatc cacgaggcgc ttggcatcgc gcgtggaagt
     1081 gcgcgcgtgc aactcggggg gcatggcggc gagacgtttg actttgccgc gggcgatgtc
     1141 gcggtgctgc cggccggcac cgggcaccag tggctgtcgg gcagcgacga tctgctggtg
     1201 atcggcgcct atccaccgga aggcacatac aatctctgcc gcggcgataa cccggccgac
     1261 cgtgacaagg cgctcatgac gattccgaag gtgcctgtgc cgcaaaacga tccggtcctc
     1321 ggcaaggatg gcggtctggc gaagttgtgg cgcgggtgat gtgcccattg ctccgctcat
     1381 tcccgcgcac caacatcgga tatatccgat gttggcgtat acgcgatggg aatccagggc
     1441 acactaaatg agccagagcc tgatcgctct ggacccccgc tttcgcgggc gtgagcggaa
     1501 taaaagatgc gttacgtgac tccgcgcgat agaaagtttg catgagcgac tgggtcaagt
     1561 tctacgactt caagcattct gtaatctacg tgaatgcgcg tcaccgcgac gtgcattacc
     1621 gcacgatagc cgaggacatc cgcggctacg tgccctcgcg cgacgcgcat gtactggact
     1681 acggctgcgg cgaggcgacg tcggccgatc tcatagccga tgcctgcgca cagctggtgc
     1741 tggtcgaagc cgcgccgaac gtgcgcgcct cgctcaccat gcgctatgcc gggcacccga
     1801 agatcgccgt gatcgccgcc gagcaggctg cggcgttgca gaaggcctcg ttcgatctgg
     1861 tcgtcatgca ttcggtcgcg cagtacctga cgcaggccga gcttgaccgt ctgcttgcgg
     1921 tcttccgcac gctgctgaag ccgggcggcc tgttcattct cggcgatatc gtgccgccgc
     1981 atttcgcggc gccggccgcc gcgctgtcgc tgctgcgctt cggcgccgcc aacggcttct
     2041 tccgggcggc ggcgttcggg ctggtgcgga ttttcgcgtc ggactatctg cgcctgagga
     2101 aaaccgccgg cctgtcgcat tacagcgaga cggcaatgct ggaacgcctg agcaaagccg
     2161 ggttcaaagc cgagcgcgcc gcgcgcaaca tcggtcacaa ccaacggcgg atgacgtttc
     2221 tgtgccgggt tgcctgacga ggcatctaaa cttcggatta accgaaaccc gcatcagcgg
     2281 cattcctaca ctttacatca aggcgcgcgg gcctattcct gccggcggcc cggtggcgga
     2341 gtggcgacgc agaagccatg caaggtcttc caagcctggt tcgattccag gccgggcctc
     2401 caatcttcaa gttttggcac agcgtccgca ctgaccctca ccggattgca aaataaggcg
     2461 cttcattgtt ccggtctgta caagtacgat gatctgtgac tgggggcggg ctgtgcggcg
     2521 acgtgaattc ctggctttca ttggcggcgc gttcgtcgcg acgccgctag ctgctcatgc
     2581 gcagggcgaa cgcatgcggc gtatcggcgt gctgttgcca agcggggccg atgatcctga
     2641 gtatgaagcc cgcatgaagg ccttcacgga acggcttgct caactcggat gggtcgaagg
     2701 gcgaaatatc cagatcgaca aacgatttgg agttgccatc gccgagcaga cacgcaaatt
     2761 cgcggtcgaa ttggtagcac ttgcaccaga cgttattctg gcgccgggga gttcagcaac
     2821 gggcccgctg ctgcaagcca ccggcactat tccaatcgta ttcgcgacca tccccgatcc
     2881 ggtcgcggcc ggttatgtgg aaagtctgtc gcgaccggga ggcaacgcga cgggatttgc
     2941 cagtttcgaa tattcgatcg gcggcaagtg gcttgaactc ctcaaagaga tcgcgcctgg
     3001 cgtaacgcgt gtcgcggtca ttcgcgacaa cacgacaccg gccggtgtcg gccaggtcag
     3061 tgctgtccaa accgctgcgt cttcgcttca cgtggaaacg tcggtaatca actttcgcag
     3121 tcccggagaa ctcgaaaagg ccgttgcggc cttcgcgcgc ccttcgagtg ccttgctcgt
     3181 ctcgcaaggg ggcggcacgg tggtccatcg caagctgatt atcgctcttg cggcgaaaca
     3241 tcggctgccc gccgtttacc cgggccgtca ctttgtggcg attgggggcc tgacatccta
     3301 cggaccggat cgcgccgatc agtaccggca ggcggccggc tacgtggatc gtattctcaa
     3361 aggagaaaag cccgccgatc tgccggtgca gacgccaagc aaatacgaac tggtcatcaa
     3421 tctcaaaacc gcgaaagcgc ttgggctggg cgtgccgccg tcgttgattg cccgcgccga
     3481 cgaagtgatc gaataagctt cggatcgcct tgggcacccg gtgccgtggc acatcccgtt
     3541 ctcgcatgac actctgcgga agttgctaag cgcttatcaa gctcagggta attgtgctgc
     3601 atcctgccta cagtcggaat aaaataagca tccagcctgg aactgaacgt caaccattcc
     3661 cgagaagccg ccatgaccgc attcaacgct gtccgcttca aagtaaggcc cgggcgcgag
     3721 caggaattcc tcgatgccca caagaaagtc cgcgccgact ggccgggtct caggcacgcc
     3781 aacatgatca agaccggcga ccgaacgttc tgcatcattg cggaatggac cgacatggac
     3841 gcgcttacaa atgcgcggcc tcatatggtt gcgacgctcg attcgttccg cgcgacgctt
     3901 gaagacctcg gtggcggcct cggcgttacc gacccggtct ccggtccggt tgtgcttgcg
     3961 ctgaaataac cggggcacga cgtccgctgc cgtcaacgtg ctgaaacctc gcgggctttt
     4021 cgcgatcgtc aactggcatc agttgccgcg ggaaaaaacc accgtgccgg gggaaccccg
     4081 cgccccccgc tcggagctgc ggctctcgcc ggacctgacc gtcaaggctg tcgaagccac
     4141 cgggctcgag accaaggcga tcgtcgacat ttcgccttat ctacggcgcg acgttcgaga
     4201 aagcggtccg gtcttagttc ttcggccggt gcgattttcc cc
//
LOCUS       DASSRL010000656         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_139726, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000656 DASSRL010000000
VERSION     DASSRL010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139726"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFL49_13465"
     CDS             <1..289
                     /locus_tag="VFL49_13465"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HET7913036.1"
                     /translation="REFDKLLIYMMAEVALKRKNKGLKLNYPEAVAIISAVALDGARA
                     GKTIEEVTKDARSALTKNDVMDGVADLVPMVQIEAVFTDGSRLVTVHTPIQ"
     gene            309..758
                     /locus_tag="VFL49_13470"
     CDS             309..758
                     /locus_tag="VFL49_13470"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HET7913037.1"
                     /translation="MAKAKTFPTLSSKVDPASEEVPLGGYVCGPDPVTFDEKKQVTKL
                     TVRNTGDRPVQVGSHFHFFEVNRALEFDRAAAFGLHLNIPSSTAMRFEPGDERAVALV
                     PFGGKRAVFGFNNLVDGATAGKDAAARKSKAVKLASKRGFRSTAAKS"
     gene            795..2516
                     /locus_tag="VFL49_13475"
     CDS             795..2516
                     /locus_tag="VFL49_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET7913038.1"
                     /translation="MATISRNEYVNLFGPTVGDRIRLGDTGLFVEIERDLRGRLGDEI
                     VFGGGKSLREGMGMDNRLTRKAGAPDLVITNVTIIDAIQGVVKADVGVKDGLICGIGK
                     SGNAQTMDGVTPGLELGLATDAISGSHLILTAAGIDTHIHFISPQQAEAALSNGTTTL
                     IGGGTGPSDGSNATTVTPGPGNIEMMLKSFEGWPVNVGILGKGHGHGKAALVEQIEAG
                     AVGFKCHEDWGTTPAVLRSALTVADEMDVQVCIHTDTLNESGFVEDSIEAFEGRTVHS
                     FHTEGSGGGHAPDIIRISALPNVLPSSTNPTLPYGINSQAELYDMIMVCHHLSPDIPS
                     DVAFAESRIRAETIAAENVLQDLGVISMFSSDSQAMGRIGECWLRAIQTADAMKTGRG
                     KLPEDAPGNDNFRVLRYVAKITINPAISHGISKVLGSVEVGKVADLVLWEPAFFGAKP
                     KLVIKNGQISWGLMGDPNASLPTPQPVYYRPMFGAYGDALARNCISFVSGAAHARGIQ
                     KRLGLKRKVIPVKNIRKVRKRDMVRNSGTPDIKVDPETFAVTVDGVHATVPPLKVISL
                     NQKYFFS"
     gene            2527..3225
                     /gene="ureE"
                     /locus_tag="VFL49_13480"
     CDS             2527..3225
                     /gene="ureE"
                     /locus_tag="VFL49_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973606.1"
                     /note="involved in the assembly of the urease
                     metallocenter; possible nickel donor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HET7913039.1"
                     /translation="MVIIETVLGNVNDREWAKRLSGASIDILELDQWEAQKNRFRKKT
                     AKGTELAVALDRGTAMRDGDILVWDASKATGIAVRIRLREVMVVHLDGLAHVEPGVAM
                     RTCLELGHALGNQHWPALIKGDHVYVPLTVDRKVMASVMNTHRFEDIRYEFVPGSEVV
                     PYLAPHESRRLFGGAEGQAHSHAHDTYAEVDTETGHVHSRAPTRSHTHSHGHSGDHVH
                     FHGSPAGLADDNTK"
     gene            3209..3928
                     /locus_tag="VFL49_13485"
     CDS             3209..3928
                     /locus_tag="VFL49_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HET7913040.1"
                     /translation="MTIQSERGGGPRTAAILARMLQFGDSMFPIGGFSFSCGLESAIQ
                     KGIVVDTATLAAYARTAVEQAARGDGIALIAAHNAATAADFDALVQIDKVVFARKLSE
                     ETRTMTVRMGKKFTEIGLQVIDLPLLRKWCECIEAGTTPGTYPISLAINFAVQNLSAR
                     EAFVVHQYGVSAMILGASLRLMRISHMDTQRILYDLNARVDADYQIAAKARLWDMAGY
                     APLTEILAAVHAKAHVRLFMS"
     gene            3946..>4226
                     /locus_tag="VFL49_13490"
     CDS             3946..>4226
                     /locus_tag="VFL49_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7913041.1"
                     /translation="MSKKKIVRIGVGGPVGSGKTAIVETITPRLLDLGIKVLIITNDV
                     VTTEDAKQVQRTLKGVLIQEKILGVETGACPHTAVREDPSMNLAAVEEM"
BASE COUNT          910 a         1180 c         1283 g          853 t
ORIGIN      
        1 gagagagttc gacaagctgc tcatctacat gatggccgag gttgcattga agcgaaaaaa
       61 taaaggcctg aagctcaatt atccggaagc tgttgccatc atctcggcgg ttgcccttga
      121 cggtgcgcgt gcgggtaaaa ccattgaaga agtcacgaag gatgcgcgct ctgcactgac
      181 caagaacgac gtcatggatg gcgtcgcgga cctggtcccg atggtgcaaa tcgaggcggt
      241 attcaccgac ggcagccgcc tggtaaccgt tcacactccg atacaatagt gcgcgactgg
      301 aggttgctat ggccaaggcc aagacctttc caaccctttc ttcaaaagtc gacccggcgt
      361 cggaggaagt gccgcttggt ggttatgttt gcggcccgga tccggtgact ttcgatgaaa
      421 aaaaacaagt gacgaagctt accgtgcgca ataccggcga ccgccccgtg caggtcggct
      481 cgcattttca tttttttgaa gtcaaccgtg ctctggagtt cgatcgggcg gcggcgtttg
      541 gcctccacct caacattccg tcgtcgactg ccatgcggtt cgagccgggc gacgagcgcg
      601 cggtcgcttt ggtgccgttt ggcggaaagc gggccgtttt cggattcaac aacctcgttg
      661 acggcgcgac ggccggtaag gacgccgctg cccggaagtc caaggccgta aaattggcaa
      721 gcaagcgtgg atttcgttca actgctgcga agtcctgagc tgcctgaagc tgtgtgtgaa
      781 ttgaaggaca tcccatggct acgatttccc gcaatgagta cgtcaatttg tttggtccaa
      841 cggtcggtga tcgcattcga ttgggcgata cagggctgtt cgtagagatc gagcgggatc
      901 tgcgcggccg gctcggcgat gagattgtgt tcgggggcgg caagagcctg cgtgaaggca
      961 tgggcatgga caaccggctg acgcgcaagg ccggggcgcc tgaccttgtc atcaccaacg
     1021 tcacaatcat cgacgcaata caaggcgtgg ttaaagctga cgtcggcgtc aaggacggac
     1081 tcatttgtgg catcggcaag tcgggcaatg cgcagaccat ggatggcgtg acgcccggcc
     1141 tggagcttgg tctcgcaacc gatgcgatct ccggctcgca tttgatcttg accgcagccg
     1201 gtatcgacac gcatatccat ttcatctcgc cgcagcaggc agaggcggct ttatccaacg
     1261 gaacgacgac cttgattggc ggaggaacgg gcccgtcgga tggctcgaac gccacgacgg
     1321 tcacgccggg ccccggcaac atcgaaatga tgctgaaatc gttcgagggc tggccagtca
     1381 atgtcggcat ccttggcaag gggcatggcc acggcaaggc tgcgctcgtt gagcaaatcg
     1441 aggccggcgc ggttggcttc aaatgtcacg aagattgggg gaccactccg gccgtgcttc
     1501 gctccgcgct cacggtggcc gatgagatgg acgtgcaggt ctgcatccac accgacacat
     1561 tgaacgaatc cgggtttgtc gaagactcga tcgaggcttt cgaaggacgt actgttcatt
     1621 cgttccacac tgaaggttcc ggcggcgggc acgcacccga catcatcagg atctccgcgc
     1681 tgccgaatgt tctcccgtcg tcaacaaacc cgacgcttcc ttacggcatc aattcgcagg
     1741 ccgagctcta cgacatgatc atggtctgcc accacctcag tcctgatatt ccgtctgacg
     1801 tcgcgttcgc tgagagccgc atccgggccg agactatcgc cgccgagaat gttctgcagg
     1861 atctgggcgt gatttcgatg ttctcgagcg actcgcaggc tatggggcgg atcggggaat
     1921 gctggctgcg tgccattcag accgccgatg ccatgaagac gggccgcggg aagctgccgg
     1981 aggatgcgcc gggcaacgac aatttccgcg tgctgcgcta cgtggcgaag atcacgataa
     2041 atcctgcgat atcgcacggc atttcgaaag tcctgggctc ggtcgaggtc ggaaaagtcg
     2101 cagacctggt cttatgggag ccggcctttt tcggtgccaa gccaaaactt gtcatcaaga
     2161 atgggcaaat cagctgggga ctcatgggcg acccgaatgc gtcgctgccg acgccgcagc
     2221 cggtctacta ccggccgatg ttcggcgcat atggcgatgc gctggcgagg aactgcattt
     2281 cgtttgtctc tggcgcagcg cacgcccgtg gcatccagaa acgcctgggg ctcaagagga
     2341 aggtgatccc cgtcaagaat atccgtaagg ttcgcaaacg tgacatggtc cgcaattccg
     2401 gcacccccga tataaaggtg gatccggaaa cattcgccgt cactgtcgac ggagtgcatg
     2461 caacggttcc acctttaaag gtgatttcgc tcaatcagaa atatttcttc agctagcaag
     2521 cacgccatgg tcatcattga aacagttctc ggcaacgtca acgatcgcga atgggcgaag
     2581 cgattgtccg gtgccagcat cgatattctt gaactggatc agtgggaggc ccagaaaaat
     2641 cgcttccgga aaaagactgc caaaggaacc gagcttgccg tcgcgcttga tcgtgggaca
     2701 gccatgcgcg acggcgacat attggtctgg gacgcgagca aagcgactgg catcgcggtg
     2761 cggatacgcc tgcgcgaagt catggtcgtc catctggacg gcttggcaca tgtcgaaccc
     2821 ggggttgcga tgcgcacctg cctggaactg ggacacgcgc tcggcaacca gcactggccc
     2881 gcgctgatca aaggggatca tgtctatgtg ccgctgaccg tcgaccgcaa ggtcatggcc
     2941 tccgttatga acacccatcg ctttgaagac attcggtatg agtttgtgcc aggcagcgag
     3001 gtcgtcccct atctcgcgcc acatgaatca cgccggttat tcggtggtgc cgaaggccag
     3061 gcgcattctc atgcgcatga tacctatgcg gaagtcgata ccgaaacggg gcacgtgcac
     3121 agtcgcgccc caacgcgttc gcacacacat agtcatggcc attccggtga ccacgtgcat
     3181 ttccatggat cgccggcggg tctcgcagat gacaatacaa agtgagcgtg gcggcggtcc
     3241 gcgcacggcg gcaattctcg cccggatgct gcagttcggg gactcaatgt ttccgatcgg
     3301 cgggttctcg ttttcgtgtg gcctggaatc cgcgattcaa aaaggcatcg tcgtggatac
     3361 cgcgacgctt gcggcctacg cacgcactgc ggtggaacag gcggcacgtg gtgacggaat
     3421 cgctctgatt gccgcacaca atgccgcgac cgcggctgac ttcgatgcgc tcgttcaaat
     3481 cgacaaggtc gtgttcgcac gcaagctctc tgaagagacg cggacaatga ccgtccgtat
     3541 gggcaaaaag ttcaccgaga tcgggttgca agtcattgat cttccattac tgcgcaagtg
     3601 gtgcgaatgc atcgaagccg gtaccacgcc cggaacttat ccgatatcgc tggcgatcaa
     3661 cttcgcagta cagaatcttt ctgcgaggga agcgtttgtt gttcatcaat acggggtgtc
     3721 ggcgatgatc ctcggcgcat cgctccggct gatgcggatc agtcacatgg acacgcaacg
     3781 catcctctat gacctcaatg cccgcgtgga cgcggactac cagatcgccg ccaaagcgcg
     3841 cctgtgggac atggcgggtt atgcgccact caccgaaatc ctcgcagccg ttcacgccaa
     3901 ggcgcatgtg cggctgttta tgagttgaac ggattggaca ttcgaatgtc gaaaaagaaa
     3961 atcgtacgta ttggcgtggg cgggccggtc ggttcgggaa agacggcaat cgtggagacc
     4021 atcactccgc gactactcga tctgggcata aaggtgctca tcataaccaa tgacgtcgtg
     4081 accacggagg atgcaaaaca ggtgcagcgg acgctcaagg gcgttctgat tcaggaaaag
     4141 atcctcggag ttgagaccgg cgcgtgtccg cacacagcag tacgcgagga cccgagcatg
     4201 aaccttgctg ccgtcgaaga aatgga
//
LOCUS       DASSRL010000657         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudolabrys sp. isolate SMAG_U488 k141_192415, whole
            genome shotgun sequence.
ACCESSION   DASSRL010000657 DASSRL010000000
VERSION     DASSRL010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968516
            Sequence Read Archive: SRR25158411
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudolabrys sp. (soil metagenome)
  ORGANISM  Pseudolabrys sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Xanthobacteraceae; Pseudolabrys.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:04:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,654
            CDSs (with protein)               :: 2,654
            Genes (RNA)                       :: 21
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 4 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Pseudolabrys sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192415"
                     /isolate="SMAG_U488"
                     /isolation_source="soil"
                     /db_xref="taxon:1960880"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.3675 N 113.5805556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFL49_13495"
     CDS             <1..702
                     /locus_tag="VFL49_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET7913042.1"
                     /translation="GAGVTLYEGIQHIRDPHKIVDAHISYIVLACAAVFEGASWLVAL
                     REFSKSKGEAGYYEAMRQSKDPPTFIVLFEDSAALLGLLIAFLGTFAADQLGMPVLDG
                     VASIGISVLLAGTALVLAKESKDLLIGEPASRETRDAILAIGQKLDGIERVQIIFTVH
                     LAPDQVVVALRLEFRDDLTAPAIEKLTAELERRIHDAHPDVMAIFVKPQPVGSQARSF
                     GRASPSIEANAASAL"
     gene            complement(718..2043)
                     /locus_tag="VFL49_13500"
     CDS             complement(718..2043)
                     /locus_tag="VFL49_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7913043.1"
                     /translation="MPFNELLALLMLVSFFVLLMAGIPVALTLATAGLLFGYIGFGAT
                     LFHLLPNRLFGVVTNYTLIAMPLFVFMGVMLEKSRLAEDLMEVIAHAAGNLRGGLGIG
                     IIVVGVLMGATTGIVGATVVTLGLLTLPTLLRRGYDRGLACGTICASGTLGQIIPPSL
                     ILILLADILNESVGTLFAAAMIPGLMLAGIYVVYILGLGIVRPKMVPAVPKAERDLLS
                     RSELLGKLFRVVLPPIALVGAVLGSIIGGIAAPTEAASMGALGAIVVTAIGRRMSWAV
                     LRDTTYSTTRITAMMMFILMCAQAFSLAFRGLQGERLVQDFFAFVPGGTSGAIWFLML
                     IIFILGFFIEWIEISYIAVPLFLPIFIAANVDMVWLATLICVNLQTSFLTPPFGWALF
                     FLRGVAPPEISTGDIYRGIVPFVGMQLVALVLCFCFPQLALWLPRTIGW"
     gene            complement(2043..2549)
                     /locus_tag="VFL49_13505"
     CDS             complement(2043..2549)
                     /locus_tag="VFL49_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HET7913044.1"
                     /translation="MTPAERLAGGIDTFIDAVGRITAWSSFALALVMGTNVLLRYGFS
                     TGSVWAQELEWHLMSPICLFGMSYALRHGEHVRVDVLFASFSERNKLLVEFITALISM
                     AVCAIVIDLSWSYVAYSWNIGEGSANPGGVPARYLLKALIPLGFALLFLQSLAQAIQY
                     FHAWRRAD"
     gene            complement(2551..>2757)
                     /locus_tag="VFL49_13510"
     CDS             complement(2551..>2757)
                     /locus_tag="VFL49_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7913045.1"
                     /translation="KNQGVIAAPLPDAVVEALRAANVKILDEAVAKDPVTKKVHDSYM
                     AYMAKFKQWSAYSESVYHGKILKG"
BASE COUNT          641 a          836 c          819 g          461 t
ORIGIN      
        1 ggggccggcg tgaccctcta tgaaggcatt cagcacatca gagatccgca caagattgtc
       61 gacgcacaca tcagctatat cgtgctcgcc tgtgccgctg tgtttgaagg cgcatcgtgg
      121 ctggtcgcct tgcgcgagtt ctcaaaatcc aaaggcgagg ccggttacta cgaggcgatg
      181 cgccagagca aggatccgcc gacattcatt gtcctgtttg aggacagtgc cgcgctgctc
      241 ggccttctca ttgcatttct cggaaccttt gcagccgacc agctgggaat gccggtcctc
      301 gatggcgtgg cctcgatcgg gattagcgtc ctgcttgcgg gcacggcatt ggtgctggcg
      361 aaagaaagca aggatctgct gatcggcgaa cctgcaagcc gtgagacgcg cgacgcgatc
      421 ctcgcgattg gtcagaagct tgacggtatc gagcgcgtgc agatcatttt caccgtgcat
      481 cttgcgccag accaggtcgt cgtcgctctc aggctcgagt tccgtgatga tctgacagcg
      541 cctgcaattg aaaaactgac tgctgagctt gagcgtcgga tccatgacgc gcatccggac
      601 gtcatggcga ttttcgtcaa gccgcagccc gtgggctcgc aagcgaggtc cttcggccgc
      661 gcgtcgcctt caattgaagc gaacgccgcc tctgcgctct agaccagtcc aggagttcta
      721 ccaaccgatc gttcgcggca gccagagcgc aagctgcgga aagcaaaagc acagcacgag
      781 cgcaacgagc tgcatgccta caaacggcac aattccgcga taaatatcgc cggtagagat
      841 ttcgggcggc gccacgccac gcaggaagaa cagggcccat ccgaagggcg gcgtaagaaa
      901 tgacgtttgg agattcacgc agatcaatgt cgccaaccac accatgtcca cattggccgc
      961 gatgaagatt ggcagaaaca gcggcactgc gatgtaggag atctcgatcc attcgatgaa
     1021 gaacccaaga atgaagatga tcagcatcag gaaccagatc gcaccactgg tcccgcccgg
     1081 cacaaaggca aagaagtcct gcaccaaccg ctccccctgg agaccacgga aggcgagcga
     1141 aaacgcctga gcgcacatca ggatgaacat catcatcgcc gttatgcgcg tggttgaata
     1201 cgtcgtgtcg cgcagcaccg cccaagacat tcggcggcca attgccgtaa cgacaatggc
     1261 gccaagcgcg cccatggacg cggcctcggt cggcgcggcg atgccgccga tgatcgaccc
     1321 aagcaccgca ccgacgagtg cgatcggcgg caatacgacc cggaacagtt ttccgagcag
     1381 ctcgctgcga ctgagaaggt cgcgctccgc tttcggcacc gccggcacca tcttgggccg
     1441 cacaatgccc agccccagga tgtagacgac ataaattccg gcaagcatca ggccgggaat
     1501 catcgcggcg gcaaacaaag taccgaccga ctcgttcaga atatccgcca gcaggatcag
     1561 gatcaggctc ggcgggataa tctggccgag cgtgccggag gcgcaaatgg tgccgcaggc
     1621 cagccccctg tcataacctc gccgcagcaa tgtcgggagt gtcagcaacc caagcgtcac
     1681 aacggtcgca ccgacaatgc cggtggttgc acccatcaga acgccaacga cgatgatgcc
     1741 gatgcccaga ccgccgcgca aattgccagc cgcgtgcgcg atcacctcca tcaggtcttc
     1801 cgcgagccgc gacttttcca gcatcacgcc catgaacacg aacagcggca tggcgatgag
     1861 tgtgtagttc gtgaccacgc cgaacagccg gttcggcagc agatgaaaca gagtggcccc
     1921 gaagccgata tagccgaaca aaagccctgc cgtcgcaagc gtgagggcga ccggaatgcc
     1981 cgccatgagg agcacgaaga aagacaccag catcaacagg gcgagcaact cgttgaaagg
     2041 catcagtccg ctctccgcca ggcgtgaaaa tactgaatgg cctgcgccag cgattgcagg
     2101 aacagcagcg cgaagccgag aggaatgagc gctttaagca ggtagcgtgc ggggacgccg
     2161 ccgggattgg ccgagccttc gccaatattc caggaatagg cgacatagct ccacgaaaga
     2221 tcgatgacga tcgcacacac cgccatggaa atcagcgcgg tgatgaactc gaccagcagc
     2281 ttgttgcgtt ccgagaagga cgcgaacagt acgtccacgc gcacgtgttc cccgtgtcgc
     2341 agcgcgtagg acatgccgaa cagacagatg ggagacatca gatgccattc gagctcctgc
     2401 gcccagacgg aaccggtgct gaagccatag cgcagcagca cgttggtccc catgaccaac
     2461 gcaagagcga atgacgacca ggcggtgatg cggccgacgg cgtcgatgaa cgtatcgatg
     2521 ccgccggcca gacgttcggc gggggtcacg tcagcccttg aggatcttgc cgtgatagac
     2581 ggattcgctg taggccgacc actgcttgaa cttggccatg tacgccatgt aggagtcgtg
     2641 caccttcttg gtgacgggat ccttggcgac ggcttcgtcg agaatcttga cgttggctgc
     2701 acgaagagct tccaccactg catcgggcaa tggcgccgcg atcacgccct ggttctt
//
